LOCUS       DACCQI010000001        58275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCQI010000001 DACCQI010000000
VERSION     DACCQI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.001"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..447)
                     /locus_tag="IVO76_000001"
     CDS             complement(<1..447)
                     /locus_tag="IVO76_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HAP7428000.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATG"
     gene            complement(558..1613)
                     /locus_tag="IVO76_000002"
     CDS             complement(558..1613)
                     /locus_tag="IVO76_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7428001.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(1623..3053)
                     /gene="gatB"
                     /locus_tag="IVO76_000003"
     CDS             complement(1623..3053)
                     /gene="gatB"
                     /locus_tag="IVO76_000003"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7428002.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(3050..4522)
                     /gene="gatA"
                     /locus_tag="IVO76_000004"
     CDS             complement(3050..4522)
                     /gene="gatA"
                     /locus_tag="IVO76_000004"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7428003.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(4522..4827)
                     /gene="gatC"
                     /locus_tag="IVO76_000005"
     CDS             complement(4522..4827)
                     /gene="gatC"
                     /locus_tag="IVO76_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7428004.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(4933..6969)
                     /gene="ligA"
                     /locus_tag="IVO76_000006"
     CDS             complement(4933..6969)
                     /gene="ligA"
                     /locus_tag="IVO76_000006"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7428005.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(6986..9220)
                     /gene="pcrA"
                     /locus_tag="IVO76_000007"
     CDS             complement(6986..9220)
                     /gene="pcrA"
                     /locus_tag="IVO76_000007"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7428006.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            9375..10934
                     /locus_tag="IVO76_000008"
     CDS             9375..10934
                     /locus_tag="IVO76_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7428007.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(11045..12976)
                     /locus_tag="IVO76_000009"
     CDS             complement(11045..12976)
                     /locus_tag="IVO76_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7428008.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(12994..13908)
                     /gene="pfkB"
                     /locus_tag="IVO76_000010"
     CDS             complement(12994..13908)
                     /gene="pfkB"
                     /locus_tag="IVO76_000010"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7428009.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(13905..14657)
                     /locus_tag="IVO76_000011"
     CDS             complement(13905..14657)
                     /locus_tag="IVO76_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7428010.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(14816..15025)
                     /locus_tag="IVO76_000012"
     CDS             complement(14816..15025)
                     /locus_tag="IVO76_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428011.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(15036..16364)
                     /locus_tag="IVO76_000013"
     CDS             complement(15036..16364)
                     /locus_tag="IVO76_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428012.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(16729..17316)
                     /locus_tag="IVO76_000014"
     CDS             complement(16729..17316)
                     /locus_tag="IVO76_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7428013.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17399..18649)
                     /gene="clpX"
                     /locus_tag="IVO76_000015"
     CDS             complement(17399..18649)
                     /gene="clpX"
                     /locus_tag="IVO76_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7428014.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(18896..20185)
                     /locus_tag="IVO76_000016"
     CDS             complement(18896..20185)
                     /locus_tag="IVO76_000016"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7428015.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(20339..21271)
                     /locus_tag="IVO76_000017"
     CDS             complement(20339..21271)
                     /locus_tag="IVO76_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428016.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(21428..22087)
                     /locus_tag="IVO76_000018"
     CDS             complement(21428..22087)
                     /locus_tag="IVO76_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7428017.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(22090..23115)
                     /gene="sppA"
                     /locus_tag="IVO76_000019"
     CDS             complement(22090..23115)
                     /gene="sppA"
                     /locus_tag="IVO76_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7428018.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(23128..23418)
                     /locus_tag="IVO76_000020"
     CDS             complement(23128..23418)
                     /locus_tag="IVO76_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428019.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            23828..25633
                     /gene="glmS"
                     /locus_tag="IVO76_000021"
     CDS             23828..25633
                     /gene="glmS"
                     /locus_tag="IVO76_000021"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7428020.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(25705..27060)
                     /locus_tag="IVO76_000022"
     CDS             complement(25705..27060)
                     /locus_tag="IVO76_000022"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7428021.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(27084..28253)
                     /locus_tag="IVO76_000023"
     CDS             complement(27084..28253)
                     /locus_tag="IVO76_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428022.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(28250..29137)
                     /locus_tag="IVO76_000024"
     CDS             complement(28250..29137)
                     /locus_tag="IVO76_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7428023.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(29326..33006)
                     /gene="nifJ"
                     /locus_tag="IVO76_000025"
     CDS             complement(29326..33006)
                     /gene="nifJ"
                     /locus_tag="IVO76_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7428024.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            33204..33725
                     /locus_tag="IVO76_000026"
     CDS             33204..33725
                     /locus_tag="IVO76_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7428025.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(34424..34989)
                     /locus_tag="IVO76_000027"
                     /pseudo
     CDS             complement(34424..34989)
                     /locus_tag="IVO76_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35168..35824)
                     /locus_tag="IVO76_000028"
     CDS             complement(35168..35824)
                     /locus_tag="IVO76_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428026.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(36392..37327)
                     /gene="whiA"
                     /locus_tag="IVO76_000029"
     CDS             complement(36392..37327)
                     /gene="whiA"
                     /locus_tag="IVO76_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7428027.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(37361..38359)
                     /locus_tag="IVO76_000030"
     CDS             complement(37361..38359)
                     /locus_tag="IVO76_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7428028.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(38356..39240)
                     /gene="rapZ"
                     /locus_tag="IVO76_000031"
     CDS             complement(38356..39240)
                     /gene="rapZ"
                     /locus_tag="IVO76_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7428029.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(39375..40106)
                     /locus_tag="IVO76_000032"
     CDS             complement(39375..40106)
                     /locus_tag="IVO76_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7428030.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(40110..41375)
                     /locus_tag="IVO76_000033"
     CDS             complement(40110..41375)
                     /locus_tag="IVO76_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7428031.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(41638..44454)
                     /gene="uvrA"
                     /locus_tag="IVO76_000034"
     CDS             complement(41638..44454)
                     /gene="uvrA"
                     /locus_tag="IVO76_000034"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7428032.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(44464..46458)
                     /gene="uvrB"
                     /locus_tag="IVO76_000035"
     CDS             complement(44464..46458)
                     /gene="uvrB"
                     /locus_tag="IVO76_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7428033.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            46717..48219
                     /locus_tag="IVO76_000036"
     CDS             46717..48219
                     /locus_tag="IVO76_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7428034.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            48221..48958
                     /locus_tag="IVO76_000037"
     CDS             48221..48958
                     /locus_tag="IVO76_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7428035.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(49326..50492)
                     /gene="dnaJ"
                     /locus_tag="IVO76_000038"
     CDS             complement(49326..50492)
                     /gene="dnaJ"
                     /locus_tag="IVO76_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7428036.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(50643..52472)
                     /gene="dnaK"
                     /locus_tag="IVO76_000039"
     CDS             complement(50643..52472)
                     /gene="dnaK"
                     /locus_tag="IVO76_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7428037.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(52523..53086)
                     /gene="grpE"
                     /locus_tag="IVO76_000040"
     CDS             complement(52523..53086)
                     /gene="grpE"
                     /locus_tag="IVO76_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7428038.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(53180..54223)
                     /gene="hrcA"
                     /locus_tag="IVO76_000041"
     CDS             complement(53180..54223)
                     /gene="hrcA"
                     /locus_tag="IVO76_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7428039.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(54458..55624)
                     /locus_tag="IVO76_000042"
     CDS             complement(54458..55624)
                     /locus_tag="IVO76_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP7428040.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(55640..55867)
                     /locus_tag="IVO76_000043"
     CDS             complement(55640..55867)
                     /locus_tag="IVO76_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7428041.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(56254..56510)
                     /locus_tag="IVO76_000044"
                     /pseudo
     CDS             complement(56254..56510)
                     /locus_tag="IVO76_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(56853..57876)
                     /locus_tag="IVO76_000045"
                     /pseudo
     CDS             complement(56853..57876)
                     /locus_tag="IVO76_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT        16108 a        12631 c        10135 g        19401 t
ORIGIN      
        1 accggtggct aactgtccat cgatacggcg aacagggatc tgtgctttat tacggtagcc
       61 gaatggttct gccattccga ccgttggaag tacaggaact tctggcatct tagcaatctt
      121 cgccattaca tgctctactt gtttttgttt gaagacaagc tgttgttcat acttcaaatg
      181 ggctaaaggt gcgataccta tgcgaagtaa tgcattgttt tctacaggct gacgatcagg
      241 tgattgtgta ataaatgttt cgacttttcc atatccaaac ttattgcctg cttttactac
      301 tagtatttct acttcttctc cagggagtgc attttcgata aataatggat aaccatcgat
      361 ttttgctacc cccattcctt cataactcaa gtcgacgatt tcgactgtat atcgttcgtt
      421 tttctttact atttgctgtt ctgtcatatt ttgctccttt aagcctattt ttcaatccta
      481 ctgaactcta ctataaatca gctaattaca caactaaaac cggcactgac cttactacgt
      541 tcagcaccgg cttttgatta attcttttga ttaggtgttt cgtctgctat ttttccgtca
      601 ccgtcgatat cttcatccgt taaacgttcg acttctttta cgaattcttt gctgacaacc
      661 tctaattctt cttcatctgt acctgtgatt gcttcatcag gaatctgatc ggtattggca
      721 aagaactcaa tgtgttgatg aagattttgg aaatgcatcg gtgcttcccc gccgtattca
      781 ccatctaagt tgatcattac tggtcgattg atatcatctt ctgtttcaac gaacaaactg
      841 cttgttttgg tatagattat tcttggatca tcgacatgtc gaccgccatt cagcatgagt
      901 gctgcaatat gcaagatctc aaagatattt gctgtcttca caatgattaa agaaaacttc
      961 ccatcatcca atttggcatc tggcgcgatc tgttcgaatc ctccaacaga gttcgtcagc
     1021 cctaggaaaa acatcgaagc atttccgata tattcccctt catcatactt caagcgcatt
     1081 ttgattggct ttactcgagg gagcatctct gctcctttcg caagataagc aagataacca
     1141 aaaatactct ttagttctga tggaacttca taagtcaatt ctgtcaaata gccacctgca
     1201 gcgatattga tgaagtaact gttatcagaa aggccgatgt ccatttttac ggtttggtgt
     1261 ttttttatca cttctgctgc tgctttaata ttatctctag gaatttttaa tgctcgagca
     1321 tagtcgttcg ttgtaccagc tgggatgatg gccattttag gacggtgttc aagcggagcg
     1381 attccattaa ccacttcgtt gattgtgcca tcgccaccag ctgccactac tagatcaaaa
     1441 cctagttctg cgactctaca tgcttcattc ttggctgaat ctggttctgg tgtagttgca
     1501 taggcgctgg cttcaaatcc tgcttcttct aatgcctgta ggatatccgc caaatttttt
     1561 ttcaataatt cttttcccga ggtggggtta taaattacgc gtgctttttt cattttcagc
     1621 ctctatcgtt tcgctaattc atctttaagt agcttattca cgacaccagg atttgcttgt
     1681 cctttagttg ctttcatgat ttgaccgact aggaagccaa ccgcacgatc ttttccattt
     1741 ttgaaatcat cgacagattg ttggttgtta tccaaaattt cattaatgat tggcagtagt
     1801 ttgttcggat cagataattg cacccaacca tttttttcaa ctacttcttg ggcttctccg
     1861 ccatttgtga tcaattcacg gaagactttt ttggcgattt ttgagctgat cgttccatcg
     1921 gcgatcaaac gaatcattcc tgctaagttc tcaggtgtca attttgtctc agctaattcc
     1981 agtttctcac tgttcaagta agcagaaact tcacccatca accagttaga tgcttgcttg
     2041 gcatctgcac cattttccaa aacagcttca aaaaaatcag acatttcttt tgtaagtgtc
     2101 aataccattg cgtcatactc tggaagtcca aactcacgga tatagcgttc gcgacgagaa
     2161 gctggcatct caggcaaact agcttttact ttttgaatcc attcgtcatc gatcacgaat
     2221 ttcgggatat caggctctgg gaagtaacga taatcactag acccttcttt gacacgcatc
     2281 aaaactgttt tattcgttgt ttcgtcaaaa cggcgtgttt cttgttggat ttctcctcct
     2341 gaaagaagaa cttttgcttg acgtttttct tcaaaagcta aacctttttt cacgaaagat
     2401 aaagagttca agtttttcaa ttctgctttc gtaccgaact cttcttgtcc ataagggcga
     2461 agagaaatat tggcatcaca gcgcatagag ccttcttcca ttttcacatc gctgacttct
     2521 gtaaattgga taattgatcg caatgcttct agataagcgt atgcttcttc tggtgaacgc
     2581 atatcagctt cagaaacgat ttcgatcaaa ggcgtgcctt gtcggttcaa atcaacgtaa
     2641 gaataaccgc caataccatg catgtttttt ccagcatctt cttctaaatg gacacgttcg
     2701 atccggattt ttttcttttt accttctact tcgatttcga tccatccgtc atgaccgatt
     2761 ggttgatcaa attgggaaat ttgatatgct tttggattat ctgggtaaaa gtaatttttc
     2821 cggtcaaaat gagtatcttt agagatttca cagtttaatg ccaaagctgc tttcatcccg
     2881 aattccagcg cagctttatt cattactggc agcacgcctg gataacccca gtcgatcacg
     2941 tttgtattgc tgtttggttc tgcaccaaaa tgagctggcg ctggagagaa aatttttgag
     3001 ttggttttta actcgacgtg gacttctagt ccaatgactg tttcaaaatt cattcgtctt
     3061 tccctcctaa aatagctggt tgttgtttat ggaattctgt tgcttgttca aacgcatatg
     3121 ccgcttgata catcgttttt tcatcaaaat aattaccgat gatctgcaat ccaactggta
     3181 atccttctga taaacctgcc ggtacagaca tacctggaag tccagccaag ttgacaggga
     3241 tcgttaagat gtcactcatg tacattgtga ttggatcgtt aatgttctca ccgattccga
     3301 atgcaactgt tggtgaagat ggcccgatga tcaagtcata atctgcaaag actttttcaa
     3361 aatctcgttt gatcaatgta cggacttgtc ccgctttttt gaagtaagcg tcataataac
     3421 cagcacttaa ggagaatgta cctaacatga tacggcgttt tacttcttca ccgaatcctt
     3481 ctgaacgtga gttgacatat acatcatcta atgatttcac attttctgag cgataaccgt
     3541 agcggatacc atcgaatcgt tgcaagttgg aacttgcttc tgatgaggca atgatataat
     3601 aaacagccac accgtatttt gaatgaggca agctgacttc ttccacaaca gcacctaatg
     3661 aacggaatgt ttctgctgct ttcaatacag cttcttttac gccaggggcc acaccttcgc
     3721 ctaagtattc tttaggtaaa gcaatcttca ttcctttgat gtcgcctgtc agtccctctg
     3781 caaaatcagg aactgaaacg cctgaagaag taccgtcttt ttcgtcataa ccgctgatag
     3841 ctgtcaatgc taatgcatta tctttcaccg tacgggtcat tggtccgatt tggtcaagag
     3901 atgaagcaaa ggcaatcaaa ccaaaacgtg agacacggcc ataagtaggt ttcatcccta
     3961 cgatgccgtt aaaagctgct ggttggcgga tacttccccc agtatcacta cctaaagaaa
     4021 ctggaatttg tccagctgca actgctgctg cagaaccacc agaagagcca ccaggtactt
     4081 ttgttgggtc ccaagcattt tttgttttct tgaaataaga agtttctgta cttccaccca
     4141 tagcaaattc atccatgttt agcttaccaa cagggatcat atctgcttga tagattttat
     4201 ccatgaccgt tgcatcataa attggttcaa aattgtaaag gatcttagat gcagcggttg
     4261 tcaagatatc ttttgttacg atattatcct tgataccaat tggaatacca gcaaggacgt
     4321 tgctttcatc aatccctttt tcatctaaag cttttgcctg ttccaatgct ttttcttcgt
     4381 tcaatgtaat gaatgcttcg acctcaggct ctgtttcttt gattcgttca aatgttgctt
     4441 ttgtcaaatc ttgtacagtg atttctttcg atacaagcat atgatgcagt tcttctagag
     4501 aacgattgtg taattctgtc attatgcgcc agcctcccca ttatcgatga ttgcgggtac
     4561 tttgataaag ccgtcttctg tttccggcac atttttcaat aattcatcgc ggttccatcc
     4621 agcttgcgcc cgatcttgtc gcatcacgtt tactgtttca accacattcg acgtaaatgg
     4681 aacaccttct gtatctactt cttccagcat ttcaaccata tcgatgattt tacctaattg
     4741 gtcggtaaaa cttgcaagtt cttcatctgc gaacgcaagt tttgctaatt tggcaacgtg
     4801 tttcacttgt tcttcactaa tcgccatact accccttctt tctaaacgag ttaaaaactt
     4861 tgctctctat tttaacacaa gagtgccgtg tctgaaattg tttattgaaa tttgtctgaa
     4921 aaaactcatt tttcattctt ctactgagcg acttttagcc aatgcatctg ccatttgatc
     4981 ttctgtccaa acttcgattc ctaattcttg tgctttggtc agcttgctgc cagcatcttc
     5041 tcctgctacc acgatatccg tttttttcga tacgctacca gtcactttcc cgccaagatt
     5101 ttcgatcgtc tcttttgctt cttcacgagt aaatcgtgtt agtttaccag taaggactac
     5161 tgttttttct ttaaaaggag actcgacttc ttgcaattgg gcatttcgaa gccctttgta
     5221 ttcgaaattg acgcctgctt gttttaattc attcatcaat tcatggactt cttcgttact
     5281 gaaataagtg actacactgt cggcaatgat atcaccgatc gtatcaagcg caataatcga
     5341 ctcatagtca cttttactca atgtttctag atcaccgaaa tgttctgcta aaatttttgc
     5401 tgctttcgct ccgacatgtc ggattcctaa cccgaaaatc aaccgctcga ccgaattatc
     5461 tttgctgttg tcgattgctg tcaaaatatt atttgctgat ttttctttta ttttatccaa
     5521 cgtcaataac tcttcttctg tcaatttgta aagatcagca acatctgcta ctagtttttt
     5581 atcatacatc tgttctagta cgcgcggccc caacccatcg atattcatcg catttcttga
     5641 aacaaagtgg ttcaaacctt ctttcatttg agcagggcat ttaggattga tgcaccgtaa
     5701 ggctacttct tcatccaaat ggacaagttc acttccacaa actggacaat gtgtaggcaa
     5761 ttggtattct tcgctgtctt taggtcgttt atctaacacg acttgcgaaa cttctgggat
     5821 gatgtctccc gctttataaa tcagaactgt atctttcaag cggatatcct tcaacttaat
     5881 ataatcaccg ttatgcaggc tggcacggct tactgtcgtt cctgctactc ggactggtgt
     5941 catgatagct gttggcgtta ctacccctgt tcgaccgact gtccattcga tgttttcgat
     6001 tagcgtttca acttcttctg gcggaaactt gtaagctgct gcccatctcg gtgctttgac
     6061 agtaaaacca agttgatctt gtagcgagaa ttcattcact ttgatgacga ttccatcgat
     6121 ttcatagggc aaatccacac gtttgtcatg atattcttca atgtatgacc acacttcatc
     6181 aatcgaatga catagacgtt tttcccgatt cgtgtggaat ccaatttttt ctagctcttc
     6241 tagcgcatca tactgggttt ttgcttgcat aggaccgaaa tcagctactg tgtataaaaa
     6301 agtgtccaga tttcttttcg ctgtgatttt agaatctaac tgacgcaaag aaccagctgc
     6361 ggcgttccga ggattcgcaa agatatcttt tccctctgct tctctgtctt ggttcaattg
     6421 gacaaatgac cgttttggca tgaaacattc gcctcttact tcgatgttca ttggttcttt
     6481 taatttgatt gggaccgacc ggacagtttt taggttttca gtgatgtttt ctcctacagt
     6541 tccatccccg cgagtcgcac ctctcacgaa atttccatct tcataacgca aagaaacgga
     6601 aagtccatct atttttagtt cacagcaata gtcaactaca cgtcccactg ctttttgtac
     6661 acgttgatca aaagctatca gctcttcttt gctgaaaacg tcgttcaaac tgtaaagcgg
     6721 aatatcatga gtgacttttt caaaaccttc taatactttt ccgcctactc gttgagtggg
     6781 agaatcagac gtgatcaaat caggatattg ttcctcaatt gctactaatt ctgcgtattc
     6841 tttatcataa acatagtctt ccactgttgg tttatcttct acgtagtatt cgcgagacca
     6901 ttggttcagt cttgtacgaa gttcttctgc tcgtttagca gcttcgtcaa tcgtcatttc
     6961 atgttccatc atttaccctc cactactaag ctttttcaat tggcgcaaag gcagccaaaa
     7021 gacgttttac tccttgactt gggaaagcca catctagttc tagatccttc gacgtcccgc
     7081 ttactcggac gactgttcct agaccccatt ttttgtgttt gactttatct ccaggtttcc
     7141 agctgttatt ttcccctcca gtagcacttt tagagggaac aaccgctgtc tctggctgtt
     7201 tatagctata tgctgttttt ttagcgaata caggtgtttt tttaggcgta ggacgcatgc
     7261 cttcgatttc cagtaactcc tgatcgattt cttctacaaa acgagaaggt cggttatatt
     7321 gcgtccgtcc atataatgtt ctggagaaag catttgttaa atacaacgct tcttctgctc
     7381 gcgtgatccc cacatatgcg agacggcgtt cttcttccag ttcgctttct tccatcaatg
     7441 ctcgagataa aggaaaaacc ccctcttcta gaccaatcag gaatacaact gggaattcta
     7501 atcctttggc tgcatgcaaa gtcatcaatg tcacttggga agcatcttct tctaaattgt
     7561 caatatcgga aacaagtgcc agatcattta agaagaccgt tagtttttct tctggtgcat
     7621 cagcgtcttc ttcattttgc tgttcgaatt gtttgtcgaa ttcttgtgtg accgttaaaa
     7681 attcctccaa gttttcgatg cgtgcttgtg cttctaatgt attttggatt tttaagtctt
     7741 ctagatatcc gctacgatcc aaaacctctt tcgtcaattc tgtcactgtc aagtatggga
     7801 tcatctgagt gacttcttgg atcatctctc caaatgcacc taattgctgt cctgcttttc
     7861 caccgatatt tgccagatcc acattttgcg ctgcttcaag cagtggccat tcatgaagag
     7921 aagcaaaact gcgtagtttt tcgatagatc ctggtccgat gcctcgtttg ggcgaattaa
     7981 cgatacgctc aaaactgatt gaatcttgag gatttgccag cacattcaaa tacgctaaaa
     8041 tatctttgat ttctttacga tcgtagaact tgtgccctcc aaccatttta taaggaatat
     8101 ttgctttcaa cagagtttct tccattacac gagattgtgc gttcgtccgg tacaagatgg
     8161 caaagtcgcc gtaattccga tgattgcttc gaatctcttc ttgcatccga tctacgataa
     8221 aacgggtttc gtctcgttca ttatctgcac ggtagtaagt gatcttgttt ccttctttgt
     8281 tttctgtcca caagtttttt ggttttcgat tgctattgtt ttcaatgact tggtttgctg
     8341 cgctaaggat gtttttcgtg gagcgatagt tttgttctag caaaattact gcagcatctg
     8401 gataatcttt ttcaaaatcc aagatgttct gcatatctgc accgcgccag ccgtaaatac
     8461 tttgatctgc atctcccacg acgcacaggt tcctaaatct acctgccaat aaattgacta
     8521 gcgtatactg tgcgtgattt gtatcttggt actcatctac atgaatataa tggaatttgt
     8581 tttgataata tgtgagtgaa tcaggatgtt cttcaaataa tcgaatcgta ttcatgatca
     8641 ggtcgtcaaa gtccatacat tgattatatt gaagctcttt ttggtaagca gcataacatt
     8701 ttgccgcaat ctcttcgtaa taactgcctt gcatttttgc aaaatcctga ggggtctgca
     8761 ggctgttttt tgcttgagag attgttccta atatacttcg aggatcatat tttttcggat
     8821 caatgttcag ttcttttagt atccgtttca tcaaggtcag ctgttcagaa gaatcgatga
     8881 ttgtaaagtt ccggttatac ccgataaaat caacatctct tcttaaaata cgcacacaca
     8941 tcgagtgaaa agtcgatacc cagacatctt cgcctccaga tgcgagaatc gcattgacac
     9001 gttctttcat ttcttttgca gccttattgg taaaagtaat cgctaggatg ttccaaggat
     9061 tgacttcttt ttcctcaatc aaataggcaa tgcggtgtgt cagcactctc gtctttccac
     9121 ttcctgcacc tgccattagc agtaatggtc cgtcagtgtg aagaacagct tctttctgac
     9181 gggggttcat cccgtttaat aattcagctt tggatgacat taacaacatc ctttctttta
     9241 tcaatcttgt taattatagc attttcttgg ctttcattaa actagctagt tcaaaatcat
     9301 ttgtttcttt ttatggattt ttccttgtca attcgttgat ttcgcgctaa attaaaagaa
     9361 agcgaggcgt ttgaatggaa aaaaaaggaa aaatcaagaa attagataag actcagatat
     9421 attttattct aaaaggaata tttgttggac tgctgacagg aatcgtcgtt agcctgtttc
     9481 gattatctat cgaaaagcta agcgatttag ttcgaagtat ctatgaaatg agtcgggaac
     9541 agccgattta cttaattggc ctagctattt gctgcatcct tgcagcattt ttcgtgggtt
     9601 atctggtcaa aaatgaaccg gatatcaagg gaagcgggat tccacaagta gagggacagc
     9661 ttcgcgggga attatcaatg aactggttct ctgttttatg gaaaaagttc attggcggtg
     9721 tactttcagt tggtgcgggc ctttttctag gtagagaagg accttctata cagcttggcg
     9781 catccattgg acaaggagct ggacaattgt ttcggagtcc atcttcagaa gaaaagattt
     9841 taatttctag cggtgcaagt agcggacttg cagcagcatt caatgctcct atcgcaggat
     9901 tactctttgt tttagaagaa atccatcact ccttttctcc acttgtttgg ctgacatcat
     9961 ttgcttcagc aatcactgct aacttcgtct ctctttactt tttcggcttg agacctgttt
    10021 tatatctagg tgaccttcct tctttgccgt tgaaatatta tggttcctta gtgatcttag
    10081 gagtacttct aggcatctta ggttttattt atcagaaggt cttgctggct ttgccaaaat
    10141 tttacaaccg attgccttta ccagcttatt ttcacgggtt cgttcctttc ttgctgattt
    10201 tgccaattgg ttatttatgg cctagtactc taggcggagg caatcaaatc gttttagctt
    10261 ttggacatgc ttccttgcca atcattatga ttatcggttt gtttgttctt cgattcgttt
    10321 tttccatgat ttcttacggt ggtggtcttc caggggggat tttccttccg atattgacat
    10381 tgggggcttt aatcggtagc ttttatggaa acattatggt tgatttcggg atggatccaa
    10441 tctacatcaa ggattttgtc gttattgcca tggctggtta tttcacagcg attggaaaag
    10501 caccgctgac agcaattatt ttagtgacag aaatggttgg ctctcttaat catctgatgc
    10561 ctttaggatt ggctgctctt gttgcttata tcgtcaatga ccttctcggc ggtaatccaa
    10621 tttacgaatc attattggaa cgtctacttg gaaatactca gaccgcctca ctaaaaggtg
    10681 caaggacaac ttttgatttt ccagtaacgg cagagagttc tttagacgga ttaatggtac
    10741 gagacttcaa ttggcctaaa aatatgctgc taatctctat acgcagagga aatcaggaaa
    10801 tcttgactca cggtgatacg atcatgaatg taggagatat tttggttatc ttgactgatg
    10861 aagcaaactt gccaagaatc aagcaggaaa tcgaggctaa atcttctatc acaaatttga
    10921 agcaatcatt ttaacgaaca tggaaaaact attttacaac aactattcat ctacactact
    10981 tgattctata tgattctata aaaaaagaag agactgggaa aaaccagcct cttctttttt
    11041 atttctatgc ttctgctgct gcaggagctt ttctcaataa accataaatc acaccagata
    11101 cgactgctcc gatagcaata aacaccaaat acatgaccgg gtgactcagt aagaatacaa
    11161 cgaaaatccc gccatgagga gccagtaatt tgatcccgaa agctccgact aatccgccag
    11221 ttaaagcaga accaataata aaacttggaa tggcacgaat cgggtcagct gctgcaaatg
    11281 gaatcgatcc ttccgtgaca aacgatagtc ccatcacgat gttcgtcaaa ccagcatctt
    11341 gatctgtttt gctgaattta ttcttaaaca aacgtgtagc aacgaaaatt gccaatggag
    11401 gtaccattcc tccagccata acagaagcca tcacgacact tccgccagtt gcaacagtcg
    11461 tagctaaagt tgctgtacca aacacataag cggctttgtt gattggaccg cctaaatctg
    11521 ccgccatcat tccagctaat aatgcaccaa gtaaagcagc attcgaacca ctcatacttg
    11581 tcaagaaacc gttcaatgca tcattgattg ctttcatagg gacattgacc agcaacatca
    11641 agaaaccagt aatcagtaag ccaaacactg gataaaagag gatcgtttta attccatcca
    11701 atgattttgg taagttcttg aataatttac gtaaaaatac gatgacataa ccagctaaga
    11761 atccaccaat cagtgcacct aagaatcctg caccgcctgt attagctaat gcaccagcag
    11821 caaatccaac gatcaaacca gggcggtcac cgatacttga agcaataaat cctgctaata
    11881 ccggcagcat gaaaccaaat gcagcttgtc cgatttggtt gaaccagcta gcagcttgat
    11941 tgtaattccc tagatttgat aattgatcat gtggcacgcc aataaattgg tcgatcatga
    12001 aagaaagcgc aatagcgatt cctccaccaa taacgaaagg taacatgtgt gatacgccgt
    12061 tcatcaaatc tttgtaaatt ctttgtccga tagtaccatc cgcatttcct tcttctgaac
    12121 tgctttcttt tccatcacca tggaatactg gtgcagtacc actaagggct agattgatca
    12181 gctcttctgg tttacggata ccatcactga caggacgatt gatcagttct tttccgtcaa
    12241 agcgattcat ctctactttt ttgtctgctg caacgattac accttctgca cgggcgatgt
    12301 cttcagcagt caatcggttt ttgattcctt ctgaaccgtt cgtttcgact ttgatctcca
    12361 cacccatttc tttcgctttt ttcttcagag catcttctgc catgtaagtg tgagcgatcc
    12421 ctgttggaca agctgtaacg gcaacaacga attttctttc tggatgagca gaagcttctg
    12481 cagcttgtgt ttcttgttct gctttttctt ctgcttcttt ttgctcttca gctgtttgga
    12541 acaagtcttg gacttcttct ggtgtatttg cttcttttaa ttttcctacg aaatctgcat
    12601 caatcagcaa tcgagatagt gctgctaatg cttgcaaatg tgtgtcgttt gcgccttctg
    12661 gagctgcgat catgaaaaac agataagttg gctgtccgtc taaggcttca taatctactc
    12721 ctttttggct ttttgcgaat aaaactgttg cttctttaac tgcactgttt ttcgcgtgag
    12781 gcatggcgat tccatcacca agacctgtag aagtttgtgc ttcgcgggct agaattcctt
    12841 ctttatatgt ttcgatatcg gaaatccggc cggcatcaaa cattttttgg accatttcat
    12901 cgattgcgcc ttttttatca gtcgcttgca aatccatgat catcgcttct ttgaccatta
    12961 aatccttgat attcatgatt atcctccttg aaactaatca atttttgtga tttcaacttc
    13021 tggcagcaac tcatcaatca attcgcgagc tgccaagtca tcagaaaaag ctgttgcgct
    13081 tccgcaagca actccccatt taaaggcttc gattggatct ttcgtacggc tgtaagcccc
    13141 aataaagccg gcaatcattg aatcccctgc acctactgaa tttttcaatg ggcgaactaa
    13201 tacattcgtt ttataagttc cttgttccgt gaagagcaaa gcaccgtctc cagccattga
    13261 gatcagcaca ttctgtgccc cttcagctag caatttttta ccatacggaa tgatttcttc
    13321 tacagattgg aaggttgtat tgtaaagctc agctaattcg tgattgtttg gtttgatcaa
    13381 caatggtttc ttaacaagtg cattttttag gtcatcacct gttgtatcaa tcacgaaatc
    13441 tgcacctttt tctcgaatga tatcaatcag ttcttgataa aatccttttc gcaggctagc
    13501 cggcgcgctt cctgacaata cgacgatatc tccttgtcct actgttccta atgcttgctt
    13561 caactcatcg atttcttcat ctgaaataga tgggcccaaa ccgttaattt cagtttcagt
    13621 atctgatttt agtttgatat tgattcttgt atcttctttt acggttgtga acaaagactg
    13681 gatttgttct ttttcaagcc acttcgaaat aaattcgcca gtgaaccctc ctaaaaaccc
    13741 tagtgctgta gattgagaat cgatccgttt caaaatacgg gaaacattga tcccttttcc
    13801 tccaggtaac ttgaagtctt ccttcattcg gttcaaatcg cctagtttta acccatctac
    13861 gcgtacgata taatcaattg atggattaag tgttactgtg taaatcattg tattgcctcc
    13921 ttaattgttg tcttttcacg gaattcatta acgaattcta atggacaacg ctcggttatg
    13981 atcgtggctg cttccaaagg tgcaacagag gtgaaggaca actggttgaa tttggtatga
    14041 tcaatcaata caaatgtctc ttcggaattt ttcatggcta aacgtttaac agctgcctct
    14101 tctgggtccg gagtcgtgaa tccttgttca agatgggcac cgttcattcc cataaatgct
    14161 ttattaaaat gaaaattttt tagttgttct atcgcattag atcctaaaac agcattagtt
    14221 gccagcttta tcgttccacc taaaataatg gtagcaagct ccaaatcaac taaatttgcc
    14281 gcatgttgaa cggaatttgt cactactgtg atctgttttc cttgcagaaa aggaatcatt
    14341 tctaacgtag tagagccagc gtctaaataa atgacatctt cgtcattaat ggtttcagct
    14401 gccgatttgg cgatttgctg tttttcgtga acgtttttga gtgatttttc actcatattc
    14461 tgctcaaaat tcaataattg ctctttctta gctccgccat gaactctttc tagaaaccca
    14521 ctattctcca gctcttgtaa atcacgtctt atcgttgatt cagaagcatt aaaaaggttt
    14581 acaagatctt gtgatttcac gattttctgc tgttcaagca agtctaggat tttttgtcta
    14641 cgttcctctg taagcatttt cacgatcacc tcaatacaaa taatagcata ttttttttct
    14701 actcgcaata taaatcagtc aaaaacgttc ataaaaatta taaatcagtc aaaaaaaaat
    14761 aaaaaaccag tcaaaaagac tggtttttta tttttttgtc tatcatcaag ccgttttatc
    14821 gttttttatt ttcgcttctt gtttttttca tataaacaga agcaggtttt ctttccttat
    14881 gaagaagata ataataaagt cccatcccgc caaacatgac caaaaaaccg atgactgatc
    14941 gaataggaaa attaggaaca aaaaagagac agacggataa taatgatgcc ggaaccatta
    15001 ttcgataata gagttgttta tccattttct tcacctcata ttttttgtaa agtaatcaat
    15061 tgttctaatt ctcggtaatc taaaaattgc acacgcagat gctgctcacc tttcttgaca
    15121 tcgaattgga tacttcgccg ctttttcttt attcgtttga aggtaacatc agagagtcta
    15181 tgtttgattt cctttaacgg taattctgac agggattctg aaggaacata gattcttaag
    15241 gataaaagat actcttgata tttttcatct gttacctcta cttctagata ttccctaata
    15301 taataacaat aagcttcgat tccattatcc aaagcaacag tcggatcgat aactttatag
    15361 cacatcatgc gcgagcgatt ctcgtcaacc caaggaaaat acgcctgagc atttagtgtt
    15421 ccgcgaacac gcctttcttt ataatctcct tgttgaatat cataactttt tgttacaaaa
    15481 aatgaagcat tctttttagg aacaggttgc atttgtgcat tctgatactg gacagattgg
    15541 atcgtatggt cagtgtaaat gtattcaacg aatggcggtt tcaataaacc gacaccattg
    15601 tcccaagcaa gatgatcaaa cccaagctgg tccctaaaat aggattgaaa catttgattg
    15661 tttggctttt gccagttaaa ggggtctcga taaagctgga tacatatgac ttcgttttct
    15721 aattccttca ctttttctac ttgttggatg tcgagaaaaa tcggtttagg gatgatttga
    15781 tgataatagc gactataata catttcccaa cacgaagtga aacataaacc ttggtaaaaa
    15841 agatcgtatc ctggaaattg gttgcaatcg actactactt ccccgttttg ttgtttcatt
    15901 ttaggtaaat gttcgatttc atcctttttc tcaaacaata gccgttgaga gatctgtttt
    15961 gtattgtgat agataaactc gctataagct cgcatatagg cgaacacccc atgagcaatc
    16021 atttttttct gaatgaagtc tcgaatcaaa aattgctttt ctttgaaaac agagatagac
    16081 aatacatttt ttatcaataa ttgattatct tccaagcgaa aactacattc gttctcgtca
    16141 tttcctacaa ctaaccaact ttcgtctctt accgatgcta gcattatttt ttcttgaagc
    16201 tttcgaagat catacggatc cgcttgttgt gtgaaagagc ggataaatgt tggctggatc
    16261 aacagacgtg tcaaaatagc ccaatcgctt atttcctgtt ttttttcttc ttcatttaag
    16321 tctgtgagct ggatagtcat caataattta tcttcgccaa tcattttctc acctctttaa
    16381 aatcttttta taatttaatt ataaccaacg ataaagaaaa actaaaactt tatgtcttag
    16441 aaaccaattt ggacgattac atgatagact aagtaaaaag aaacaaagag gtgtttacaa
    16501 tggggttgaa atttgaacgt acagataatt tagataaact tttcgaatca tttgctgtcg
    16561 atccggaaaa aacagttgca ccaaaagaag agatcgatcg atttctaaaa ccacagccac
    16621 aaaatgaaaa gaaaaaagac aagaaacaaa agtaagttaa gctcaaaaaa agacttacca
    16681 gccaaacttt tatgaaagag tttgactggt aagtcttttt tattcttttt atgattcaat
    16741 tgcttgttcg attgctgccc acgcttcttc ttttccctgt tttgtaacgg aagaaaagag
    16801 aataaacgtg tcattgcgat caaaatccaa cgcttttttg atttcgctct catgcttgtt
    16861 ccatttacca cgagggatct tatctgcctt agtggcaaca acaattacag ggatatcata
    16921 atacttcaaa aattgataca tctggatatc ttcttgagaa ggcttatgac gcaaatctac
    16981 taaagaaacg accgcacgta gctggtctct agtcgttaaa tatgtctcga tcatctttcc
    17041 ccattttgct cgctcagttt tggatacttt tgcataccca tatcccggaa catcgacaaa
    17101 atgtaatgcg ttctcgatca aataaaaatt caatgtctgg gtctttcctg gcttaccgga
    17161 agtacgggct aaattctttc gatcaatcaa tgtgttgata aaagaagatt tccctacatt
    17221 tgatcgtcct gccagagcta tttctggcaa ttgtgtatct ggatattgtt ttggtgccac
    17281 tgcacttatg ataatttcag cttgatggac tttcatggtc tctctcctta acaaggtgat
    17341 gaataaagca agcggctgtc gcaaaagcca aaatgacttt tacggcagcc tcttttcatc
    17401 aacccgcttt tttctctgaa tctttataga ttactgttgg ctcaccagac aattcagctg
    17461 cttcttttgt gataatcact ttttcgatgt tttcatttga agggatatca aacatcacat
    17521 ccatcatgat ttcttcaatg atggaacgaa gtcctcgagc accggtattg cgttcgattg
    17581 cttttttcgc aatggctctc aacgcttcag gttcgaattc aagttcggta ttatctagag
    17641 aaagaagttt ttgatattgt ttgactagcg catttttcgg ctcagtcaag atccgtacca
    17701 gatcatctgt tgtcaatttc tctaaagctg ccataacagg taatcgtccg ataaactcag
    17761 ggatcaatcc gaattttagt aaatcttcag gaatgatatg ctgcatgaca ctttcgtctt
    17821 cagaaagttt ttggttttgt gtaccaaatc caatcgtctt ttcgcccata cgattcttca
    17881 caatcgtttc gatgccatca aatgcaccac ctacgatgaa caatatgttc gtcgtatcaa
    17941 tctgaataaa ttcttgatgt ggatgtttac gtcctccctg aggtggcacg cttgcaacag
    18001 ttccttctag aattttcagc aaggcttgtt ggacaccttc gccggaaacg tcacgagtaa
    18061 tagatacatt ttcgctttta cgcgcaattt tatcgatctc atcaatatag atgatccctt
    18121 tttctgcacg ttccacattg taatcagcag attgaagcaa tttaagcaaa atgttttcta
    18181 catcttcccc cacataacca gcttctgtca aacttgtcgc atctgcgatt gcaaaaggta
    18241 cattcaatgt ttttgctaat gtttgtgcta aaaatgtttt accagaccct gtaggtccga
    18301 tcaagcagat attgcttttt tgcagttcaa catcatctgt gttttcttgt gcttgctgat
    18361 tgacacgttt atagtgattg tatacggcaa cagctaacga gcgttttgcg cgatcttgtc
    18421 caatcacgta gttattcaat acttcaagaa tttctagtgg tttagggact tcagtgaatt
    18481 cgcgtacagc ttcttcataa aattcttcat caatgatttc tttacatagg tcgatacatt
    18541 cattacagat gtagacccct ggaccagcta cgatcttttt cacttcttct tgggttttcc
    18601 cgcaaaaaga acagcgtacg gtctcattgc tacttggatt gtcatacatg gtcttcaccc
    18661 cttaacttgc aaaaagtatg caagaaacta tcaaagactt tacagtcttt tactatcata
    18721 acatagttta gtaaaaagaa aaagtgttta acaaaggatc aactgaccta tacctttgca
    18781 ttttttactc ttaacaaaaa ataaggaact gtgacaaaat cgtgtcacag tcccttattt
    18841 cgaataaaca attttctaga agtagcttct tcagaaatga tttattactt gatttctatt
    18901 ctttcgcttc tgtcgctgta cctgtgatca attcgattgc ttttttcatt gtcacatcat
    18961 gttccagcat atcttctgtc aacacgcgtt tgatttgttc gattggcatg ttgtatgttt
    19021 ctgataattc ttggatttct gcattgattt cttcttctgt tgcttgaaga ttttcagcag
    19081 ctgcaattgc ttcgatcaca agatttgttc ttgtgcgcat ttctgcttca ccttcgaatt
    19141 gtttatgcaa gtcttcttct gtagaaccag ttaattgata gtacatttca ggagagatac
    19201 cttgacgttg catgttgttc aagaattcgt ccattgaacg atgtacttca tcatgaacca
    19261 tcacgtgtgg caattcaacg atttctgcgt tttcaacagc catacggatc gctgcttcat
    19321 cttttgcatc gtccgctgct ttttcttttg tttctactaa ttctttgcga tatttttctt
    19381 tcaattcgtc taatgttgat acttcgtcat caacatcttt tgcgaattcg tcatctaatt
    19441 caggcacttc ttttgctttt acttcatgga ttgtcacttt gaagactgct tcttttccag
    19501 caagatcttc tgcttgataa tcttctggga aagttacatt tacatctaga ctttcacctg
    19561 atttatgacc aactaattga tcttcaaaac ctggaatgaa tgagcctgaa cctaattcaa
    19621 gtgagtaatt ttcgcctttt ccgccatcaa acgcttcgcc atctacaaag ccttcgaagt
    19681 cgattactac tgtatcgcca ttttcagctg cagcatcttc tttgataact aattcagctt
    19741 gtgcttcttg ttcacgtttg atacgtgcat ccacgtcagc atctgttact tcacggtctt
    19801 gtttttcaac aactagatct ttatattgtc ctaattttac ttctggtttc actgttactt
    19861 cagcagtgat tacccagtct tgaccttttt ccatgctttc aacatcgatt tttggttgag
    19921 aaacaggatc gatgccagct tctttgacag cagcttcata agcttctggt aatacagcat
    19981 tcaatgcatc ttcatataaa gcttcttcac cgtacatacg gttgaatact tgacgagaaa
    20041 ctttcccttt acggaatcca ggtacgttca agcttttttt cactttatta aacgcagttg
    20101 ttaatccttt ttggatttcg tcttgcgcaa tcgtaaattt taaaacacca tcattcgtgc
    20161 cttttttttc aaatgttgca gtcatttaat tccctccgag tttagaaatt cattttatac
    20221 atcaaaaacg tttcatgcat acctttaaat agtacactaa ccctttcaaa ttgtaaacat
    20281 attgaatgga tagaatcctt gtttttcata acatttttat ctttttcact gataaaatct
    20341 aatggaagaa gatcatttgc tgcaaaattt tttctctttt ttttaaaaaa tcttgttttt
    20401 catcttctgt accttcatac atcgtttttc ctgtacacgc ctctacagtc agttgaaacc
    20461 agcgcttcgg gtcttcgatg tatgtatcat agacaggata aacaattgca gattgcaacg
    20521 tgaattcttc atttaacacg gaactaagca gcgcatcttc attaccgtaa cgatctgaaa
    20581 atacacataa ttgctggtaa cttgcctgct gctgcggctt tggcagatct tttggtatga
    20641 ctttgactaa cttttcttca atcgtcaaat agaaaacagg atggcattcg ttcaattgcc
    20701 taagtgtctc cagtaatttt gcccttgcta aaatatggac ctctggcgat acaataagtt
    20761 tactcgctgt ttggataaac tcatgataag gaagctgttc gatctcatca atagacgcaa
    20821 gctgacgtgc aggttccaac gaaggaagtt cctccgattt attgtggagt gccttgatct
    20881 tctgcctctc catttgtccg gataattctt ctgcaaattg gattttttca atgacccgct
    20941 gctcgaaagc ttcataaaaa tctgtacttg ctatcaattt tctagcatat aaaaaatctt
    21001 gtgtactgat taacaagtca aaataaaatt cagcaaactc ctcattttga agatactgtt
    21061 tttcatgcaa gagtgcttgc ttcaatgctt cttttttctc gtccaactcg taaagacaaa
    21121 aaacaagcag gctattagcc tcaaagcttt ctttcaactc gtaagctcgt tgaaataatt
    21181 cttttgcctg ctgatattgc tggtttgcta gtgcttgttg tgctactcgc attaaattct
    21241 catatttttc tggaaaatca atcggcttca cgcagtgtcc tcctcttgct catgttcagc
    21301 tcatgttcaa tagtagcaaa acaaaaaaag catgacaatt aaaaataact gaagcaccct
    21361 ctaaaagcta gattttagat ctgactttta gaaggtgctt tcaactgtta ctgcttttta
    21421 ttgctcatca gctgttgact tcctggttga atgctttgat cagattgatc gtaacaacac
    21481 ttgtattagt gaaaaaatcg cctagatgtt gctttttctg attaaagatt gctggaagat
    21541 accctaacat cagcaaagga aattttagaa tgaaccggca agcaccttct ctaactaaaa
    21601 cggttgacca gctaagttct tcttcatcaa agctgatgac tcgaatacca aagatcattt
    21661 tcccaatcgt ttgaccatga ttgaatttcg tcaataaaat aaaatacgct agataaataa
    21721 gtaaggctgt gaatccgtag atactcagat aagaatctga aaaattaagc caacctagat
    21781 ttttggcaat accaagtgtt aaacgcgtaa tagctccgat acacaaaaga tcgaccaaga
    21841 aagcaaacat tcggataaaa aaacctgcat agaaatactt aggaaaatca tttgcttttg
    21901 gcggttcatt tcttgtgtcc gtatactttt tccatttacg ttgtttcttt ttgatttctt
    21961 cgggcgtcaa aggctctctt tcaaaagagc tcagtacctt ttgggaaaag ttggagtcag
    22021 gtcctacagc aggctgctgt tgcttatctt tttcttgttc cttattttca tttgaaagct
    22081 ctgacatgct tactcacctc cgtaataata cattgctttt ggtgattccg ctgtccctag
    22141 attctcaaga acggataaga tctgactagt ctcagaaggc ttcaatccct ggaattctgc
    22201 tagtttactt cctaaccatg tactagcaaa cccagatggc gtggaagaat attctactag
    22261 ttccgcatct tcaagctgtt gttcctttcg catagctttt aaagcatctt caggaaatcc
    22321 caattcatcg actaaaccta cttcttttgc ttggacacca tcataaatcc ttccatcagc
    22381 aatttttttg acttcttctt cagatttatt ccgtccctcg ctgacgattg ctacaaaacg
    22441 actatatgcg ctgtcgatat atgcttgaag caccgcttct tcttcttttg tctctggtcg
    22501 cgttcctgaa cccatatctt tcaacgctcc gcttttaact gttgtatctt ccacacccag
    22561 tttttctaga agtcctgaat aattcaatcc tgacatgatc acaccaattg agccggtcac
    22621 tgtttcttct gttgcaaaga ttttatctgc ttgagcagaa atatagtagc caccgctagc
    22681 tgccatgttt ttcatgctga catacattgg gatatcatgt tctttacgaa tcgtatccaa
    22741 tttcttagcg atttccgcac tttcgtagac accgccacca ggagaattta cttctaaaaa
    22801 gatacctttg actgttggat cttcttcaat cgccttcagt tgttccataa aatttttatg
    22861 attgtaacct tcagaagcaa ataaacctga agaccctccc gcagcaatcg ttccatctac
    22921 tgataatttt acgatttttt gcgtactgtc tccctcttcc aacacatttg aagctagttc
    22981 ttcatcccca tataaccaac tgttcaatcc ctcaatttga tcgttttcct gaggcttagt
    23041 caaactagct gaaactaacg aaaccacaaa taatcctgca gcaatcagaa ctgctgccca
    23101 acgtctctta ttcattcttt ctcctttcta atacaagttt tttatgacga tcacagtttc
    23161 tgactgctca gaagtgtcga accgctctgc tttttcttcc cgcgcatacc cggaaaacga
    23221 ctcttttgtc aattcatctt ctaccgtaaa ctcacgagct tcagcaactt ccaaggcatc
    23281 aggtgcgatt tcttggatgc tatcccagcc agtatctaaa aatattttcg tatcatctga
    23341 tacaccagct gatgcttcga atttggacaa ttgttttttt aacattccca gtgtcatgat
    23401 ctcaattttt gaatccattt ttactcctcc tctatccgat tactttctat tgtaccgaac
    23461 tcctgaaaaa gatgatagat aaaaacccat tattttttga cataaaaaga gcatttgaca
    23521 aagcatttga tatataccaa tttatttttt attctttttt tgttgacaag aacattcgca
    23581 aaaggttatg attttaaatg tttccagata aaaaacaaaa gaggaggata gttatacctt
    23641 ttaggatagc gccaggtctg tgtaaacaga tgacgaggag agggtttatc gaaagattcg
    23701 gcggatggcc ctagggactg cactctacag aaaatccaca aaaaatatct gcaaagataa
    23761 aacaaaagga ttttcctgca gctggaagca ttcatacgaa ttcattatag aaagtaggat
    23821 gttttttatg tgcggaattg ttggaatgat tggattaaaa aatgtaacac caggattgat
    23881 tgacggtcta gaaaaattag aataccgtgg ttatgattca gctggtatct ttgtctcaga
    23941 tggcacaact gattatctgg tcaaagcaca aggcagaatt caaaatttga aaaacaaaat
    24001 aaatattgat actacaggaa atataggtat tggtcataca cgctgggcaa ctcacgggca
    24061 accttctgaa gaaaacgccc atccacatac ttcccaaagt ggacgcttcg tgctcgttca
    24121 caatggcgta atcgaaaact ttgaagaact taaaattgct tatttagcaa atgaccactt
    24181 tattggtgag accgatactg agattatcgc tcatctaatt gaaactttcg cgaaggatac
    24241 tacaacgcaa gaggcgtttt taaaagcttt gcgggtcatc aaaggttctt acgctttcgc
    24301 tttgattgat cggacagcac ctgatgttat ttacgtcgct aaaaataaaa gtccactatt
    24361 aatcggactc ggcgatggat tcaatgtgat tgccagcgat gcaatggcta tgcttgctca
    24421 tacgaaggaa ttcgttgaga ttgaagatga agagatggtt accgtcacat cagaaaaagt
    24481 tattattcaa aattttgcag gtgacatcgt agaacgttct tcattcgaag cacaagtaga
    24541 ttcttcagat atcgagaaag gtacctatcc tttttatatg ctgaaagaaa tcgacgaaca
    24601 gcctattatc atgcgtcgta tcgctcaaaa atacgtaaca gaagacaatc gtgtctctct
    24661 tgatcaaaag ctggtcgata cattgtctga tagcgatcga atctatatcg ttgcttgcgg
    24721 aactagttac catgcggggt tagcaggtaa acagactctg gaaaaattga cacagatccc
    24781 tgttgaggtt catttagcca gtgaatttgg ctataacaca cctcttcttt caaaaaaacc
    24841 attctttatt ttcttgagtc aaagtggcga aacagcagat agccgccaag ttctggtaaa
    24901 aatcaaccgt ctcggctacc cttcacttac gattacaaat gttgctggtt ctactctttc
    24961 gcgggaagca tcctttacac tgctattgca tgctggaccc gaaatcgcag tagcatcaac
    25021 gaaagcttat actgcacaaa tagctgtact aactttatta gccaaagcta tcggtgataa
    25081 aaaagaattt gaaacttctc ttgtattcga tatcactcat gaattgagtt tagttgctaa
    25141 tgcgatggaa tcggtcatcg cccaaaaaga ttatttagaa gagttggcag aagtatatct
    25201 cagcgataca agaaatgcct tttacattgg acgtggcgct gactacaacg tttctcttga
    25261 agctgctttg aaattgaaag aaatcagtta tatccaggca gaaggttttg ccgctggcga
    25321 attgaaacat ggaacgattg ctttgatcga agaaggaaca ccagtgattg gaatcatcag
    25381 tgaagaagtg actggtgcgc atactcgagg aaacctgaaa gaagtcgaaa gccgtggcgc
    25441 taaaacacta gttattgtct cagagggatt agaaaaggaa agtgaccagc tggtgctacc
    25501 aactgtccac ccttatctaa ctactctaac cacagtcgta ccgactcaat tacttgcata
    25561 ttacgcaacg ctattacggg gattcgacgt tgacaaacca cgtaatttag cgaaatcagt
    25621 gacagtagaa taataaaaga acaaggacca gtgtcagaca tctgacactg gtccttgttc
    25681 ttttattttt tatgaaaata tctcttaatc cagtccgatt tcttctttga ctactgccgc
    25741 gattttatcg acatagtaat taactttctc atcagtaggc gcttctgcca tcactcgaag
    25801 taatggttct gtaccggaag gacgaactag gattcttcct tctgatccca tttcagcttc
    25861 tgcctcttca atcacttttt tgattgccgg aacatccatc gcaccgtttt tatcacttac
    25921 tcggatattc actaattttt gcggatagat tgttacctct gctgcaagct ctgataattt
    25981 cttacctgtc tgtttcatga tattcagtaa ctgaattcct gaaagcatcc catctccagt
    26041 tgtgttaaaa tctaagaaaa tcatgtgccc agactgctcg ccgccaaagt tataatcgtt
    26101 tttgcgcatt tcttctacaa cgtaacgatc accaacttgt gtaacgacat ctttcaaacc
    26161 gattccttcc accgctttgt ggaatcctaa gttactcatg acagtcgtaa cgatcgtatc
    26221 ttttttcaat cgattttttt ctgccaagta tttagcacag ataaacatga ttttatcacc
    26281 gtcgacaatg tttccaagtt catccaccgc aataatacga tcgccatcgc catcgaaagc
    26341 tagtccagca tctgctcctt tttcaacgac catctcagct aaacgttctg gatgggtaga
    26401 gcctacgcca tcattgatat ttaacccgtc tggatttgtg cccatggtat aaaaatctgt
    26461 ttcaagatcg gcaaacaaac gattaacact tgttgaagtt gctccgtttg ctgcatcgac
    26521 acaaatggtc aaaccggaaa ggtcaccgtt gatcgtttgt acaaggaatt gagaatattt
    26581 caataagcct tctggaaatt cttctacagt tcccaatcct tcagctgatg gacgaggcaa
    26641 ttcatccact tcagcatcta ataatgcttc aatttctagc tcttggtcat ctactagttt
    26701 aaaaccatct gcaccgaaaa atttgattcc attatcttgt gctggattat gagaagcaga
    26761 aatcatgacc cctgcgcttg ctttttgcaa acgtgtcaga taagcaacac ctggtgtaga
    26821 gatcacacct agctggaaca cttcgatacc caccgataaa agtcctgaaa tcagcgcttc
    26881 ttctaacatt tgacctgaga ttcttgtatc ccgccctact aacacgcgtg ggcgttttcc
    26941 ttcttcttca tgctggctta ggacataacc tccgcaccgt cccagcttaa atgcaagttc
    27001 tggcgtaagt tccttgttgg cttcaccgcg tacgccgtct gttccaaaat atttacccat
    27061 agttgtaaaa ttactccttt aacttaattt tctgaggaat ttgttctgct catttgtgtt
    27121 gattcgcttg tttctgatgt ttcttctgta cttgtggtcg agctgcttgt cccagattcc
    27181 gaactggaag aaatggttgt tgtcgatggt tccgttcctg aatcagcaga tgaaatactt
    27241 ctactcgtac ttgtattttc ttgtgtccga ctgctcgtcg aagcagagct actgcttgac
    27301 gtttgcaaca cgggtgtaat ctccacacta actgattcgg gcttgatact taacccctct
    27361 tctactggta tttcaatttt acgagttgtt cgatgattga tacctgagac attgatcggt
    27421 acagcaatac tgtcaccgat cgatgcaagc aaatcgctag agccgcttac ttctgcttcc
    27481 agttttgaaa aactgaaatc atagctttct accccggcag ctggtgttcc ttgctgtatc
    27541 ccataaagtc gtaccgactt gcttggcttg atcgaatcag ctctcactgt tacttgcact
    27601 ggatcagaga cgatacttaa tacttcccct gctgcattca gcgcctgtat tgggacctca
    27661 ctattaatcc catcatctgt gaccatcgaa gcgtccacat cagctactac tcgtttgatt
    27721 tctgccaatg ttttgtcacc tgtagtgata gagacttttt taggactaac ggaagtatca
    27781 tcaagttcat atccagatgg tgtgaacgtt gaaggaatca acgtttccac atcgaaatct
    27841 tttattacct ttttctcaac cgttactgta atagtcgatg gttcgatctt tgctgtgaca
    27901 gcactgctca aattttgaat ttttagcgga acctcatgtg ttccttcgcc cagcttagtg
    27961 atatctgctg tgacgcggaa catccgtgta tcttcgtttg cctctgcatt caactgaact
    28021 cggtttgcac tggaaagttt gacagtcact gcattttcat atccatggat atagtagcgc
    28081 tttgaatcat aagaaatctt gattggcacc ccagtaactg tttgattata cgcttcattt
    28141 ccagaaagcg tatttggaac ggactggcca tttgcattga agtacagcgc aatactgaac
    28201 agcaatgcaa taaaactgta aagaatgctt gtccgtttct cttttgagat catttgcgat
    28261 cccctccctt agtcataccg tctaggaaag attgaatcac gtttttcttg ttttttgttt
    28321 tctcttcagc aatcagttct ctacgcaaaa ttgccaaata ttcttgctgg gataagccag
    28381 ccatcagttc attatctaat gtaatgctga catccccagt ttcttcagag acaacgatgg
    28441 taatcgcgtc gctgacttcg cttataccga cagctgcccg gtgtcgagtc ccgaattctt
    28501 ttggaatcaa catactttct gatagaggaa gataggcgct tgctactgcg atctttcctt
    28561 cttttacaat aactgcaccg tcatgcagcg gtgtattagg aataaagata ttgatcagca
    28621 gctctccagt gatatctgca tctaatgcaa ttcctgtctc aatatactca tctaatccag
    28681 tatgccgttc gatagtaatt agcgcaccga tttttcgctt ggacatatat tggatggctt
    28741 tgtcaaaaga caagatcatc ttctcatctt cctgctgttc gctttttgat tgtttgaaaa
    28801 gcgaactgcg tccaagatgt tccagtcctc tccggatttc cggctgaaaa atcacgatag
    28861 cagctatcac cccgtacgta atgacttggt tcatcagcca agacaaggtg tgcagaccaa
    28921 tgatttccgc tacgattcgg ataacaataa aaatagctac acctttcaga agctgaatcg
    28981 cttttgtgcc tcgcagcagt tggatcagtt tgtatacaag ataccagaca accagaatat
    29041 caagtatgtt caccaaaaaa ttcagagaga acaagtcacc ggaaaacatt tgctgccaat
    29101 agtccaaatt aaaaagttcc ccgatttgaa atgacataaa aagcctctct tctcttttga
    29161 gatttcctct ctagtatatc atatgtttta attttagagg ctgatctgct cgtatttttc
    29221 ttgagaaaaa cgcataattt tcaggaaaat tgcaagtatg cgttttattt ttctctgcaa
    29281 taaatatttt taaacttcag agaattattc attatatttt ttctgttatt tttccttttt
    29341 ttcttttgta gcttgttcca tttttttacg tcgttctgct ctggcagctc ttctttcttc
    29401 tgcttcaagg tccgtcgttt tttcttttct agtcttaacg ggtacatcga tagattcctc
    29461 tggttcaagt cgtttcttga ttttttctaa ttctcccgtc agtgcagcat agttgtaaaa
    29521 acgtgtttta gcgtcttgga tagtcttttc aaataaagct tttgcaagat ttggatttgc
    29581 gttctctagc gaggaaaagc gaacttgttt cctcatgaaa tcaggcatag cagagaaatc
    29641 aggttttttg tagtccaaag tcattgggtt cttgtttttt tcgaccattg cagggttata
    29701 tcgatataat gaccaatagc cagaagaaac agcttctttt gcttctttaa gcgattcttt
    29761 cattccacca agcaaaccat gtgtgataca aggcgtatag gcaatgatta aagaaggtcc
    29821 aggatacttt tctgcttctt caaaagcctt tatcgtctgc atttgattgg cacctgaagc
    29881 aatttgcgcc acataaacat ttccatatgt catcgccatc attcctagat ctttctttga
    29941 agcatacttt ccactagccg aaaatttagc gattgccgaa gctggtgttg cttttgaggt
    30001 ttgaccgcca gtattggaat agacttcgtt atccaaaaca agaatattca cgtctgctcc
    30061 gctggcaagt acatgatcga tcccgccaaa accaatgtcg taagcccatc cgtctccacc
    30121 aatcatccat tggctgtttt ttacgaatag atcctttctc ttgtatatat cctccagttc
    30181 agggacaatt gatcgttcgc tttcgatcgc agcttgcagt tttgctgctc tttgctgcgt
    30241 tccttcgctc tcaaacatgt gtaccaacca gtcttccatc aattctcgta gttcttctga
    30301 tacttttggc aatacttctt tgattgaact tgccaaagcc tcgcgacgag tctgtgccgc
    30361 tagaagcata ccataaccaa attcagcatt atcttccaat aaagaatttg accatgcagg
    30421 cccttgacca cattcattgg tcgtataagg agaagcagga gaagcaccgc cccaaataga
    30481 agaacagcct gtcgcattcg cgatcatcat tcgatcaccg aacatttgtg tcaataattt
    30541 tacataaggt gtttcgccac agcctgagca agcgcccgag aattcaagaa gcggttgatt
    30601 gaattgtgta gacaagacag tattctttcc tttgactgga tttgcttttt gtcttaatgt
    30661 cattgcaaaa gcccaattca cagcttgttc tttctgttct tcataaggct tcatcactaa
    30721 agctttttct tttgcgggac aagcctctac acacagacca caacctgtac agtcttccac
    30781 tgaaacttgg atacggtatt ttacgccgtc tgcgcctcgc atttctctca cgataaatcc
    30841 ttctggcgct tcttccattt cctcttcatc tgctaaaaat ggacggatgg cagcatgagg
    30901 acaaacaaag gcacattcat tacacatcgt acagcgatca ctaatccact caggtacttc
    30961 taaagcgacg ccccgctttt ccatagaagc cgttcctaaa ggcatttcgc cagttgtcat
    31021 tccatttgct acaagtgtac caacagagag ttgatttcct tcctgtgcat tcactggttc
    31081 aagtatttct tgtatataac cagtgttcct tttattgatt ttttcaggca tctccaacgt
    31141 tctccaatgg tctgggatcg cgacttgatg aagcagttcg gtcgtccgat cgattgcttg
    31201 tatgtttttt tctacgatag ccattgattt atgtccatag cttttcaggg cttcttcttt
    31261 tagcagcgga agcacctcat caaaaggaat gatgtcagct aatttaaaaa aagccgtttc
    31321 cattgccgta ttgatccggc gtcccaaacc gacttcttgg gccaatctca tggcattgat
    31381 cgtatagaat ctaatgttat tttcagccaa gtaacgtttt aattttaagg gcagatgcgt
    31441 ttctaattgt tcgtcgctcc aaagagtatt caataaaaaa atgccacctg gcttcaatcc
    31501 tttgaccaag tcatagctat gcaggtaagc tggtgtgtga caggcaacaa ggtccgcatg
    31561 ctcgacaaga tacgctgaac gtatcggcgt atctccaaaa cgaagatgag agacagtcag
    31621 accgccagat tttttcgaat cataataaaa atagccttgc gcatatttat cggtgtgatc
    31681 tccaataatc ttgatagctg atttatttgc tccaactgtt ccgtcagatc caaatcccca
    31741 aaacttggcc tggaatgttt gcggttctgt caaatccaga ctttcctttg gttccaaaga
    31801 ttgataagtc acatcatcca cgatccctat ggtaaaccgt tttttgcgta tgcttgggtc
    31861 ttttttcaat tcatcaaaaa ctgctgagat ttggtcggga gtcacatctt tagaccccaa
    31921 tccgtagcga ccaccaataa cagccgggcg aagttcagaa tcatataacg cactttgaac
    31981 gtctaataag agaggttctc cacctgcccc tggttcttta cttcgatcaa gaacagcaat
    32041 tgtttttact gtatgaggca acttttgcac gaatgtttcc actggaaaag gacgataaag
    32101 atggatattc aaaaaacctg ttttccgtcc gtttgcattc aagtaatcaa tcgtttgttc
    32161 aatcgtctgt gcaactgatc ccatagaaac aatgacttct tccgcatcgt ctgctccgta
    32221 ataagtcacc aagtcataag aagttcctcg taatcggttg atctctttca tatatttttg
    32281 aacgatttca ggtaatttat cataataggg gttgatcgtt tcccgttgtt gaaaatggat
    32341 gtctggattc tggttcgtac cactaacagt cggatgattg ggattcatcc ctcgtttacg
    32401 aaatgaagct aatttctcct gattgatcag aggagctaat tcttcatatt ccaaaacttc
    32461 gattttttga atctcgtgac tcgtacggaa tccatcaaag aaattcaaaa aaggtacttc
    32521 tccttcaata gaagcaagat gggcaacagg tgctaaatcc atcacttctt gtacactgct
    32581 ttcagctaac atagcaaatc ctgtttgtct tgcagccatc acgtctccgt gatctccgaa
    32641 aatattcaac gcatttgtcg tcactgcccg actagcaaca tggaaaacac ttggcagtag
    32701 ttccccagca atcttgtaca tattcggtat catcaaaagc agcccttgag aagcggtata
    32761 tgtagttgtc agtgcaccag ttttcaatga tccatggacc acaccagctg ctcctgcttc
    32821 ggattgcatt tctactactt ttactggttg accgaataca ttttcttttc ctgctgctga
    32881 ccattgatct actaattcag ccatggtcga actgggtgtt attggatata ttgcagcaat
    32941 ctcggtaaat gcataagaaa tatatgcggc tgccgtattg ccatccattg ttttcatttt
    33001 tcgcatgatt catcatcctt gttcatattc tctcttatta tctgttccat tataacgtaa
    33061 aacattccat tttttctttt atttataaaa atatttcaca tatgtctatt tgaattggtc
    33121 atctaatttt tagacaaaca gtctaaatcg ttgccttttt ttgtcagaca tgtataaact
    33181 ataagcgaaa gaggtgtttt aacatggata tatcagtgat tgatgcaacg aaaacaaata
    33241 cacaaaaagg aatcctttat ggttcatcga atgctcctaa aaaaatggtc gaattcatca
    33301 acctatcttg tccgtattgc cgacaatggt ttgaagaatc ttatgagcta ttagaagaag
    33361 cagtccaaag cgggcaattg cagcgagtga tcaaattgtt cgataaagaa aaagaaagct
    33421 tgcagcgagg aaacgtgatg caccggtatc tcacaatcag cgatggtcaa aaagcgatca
    33481 aagagatcaa acaaatattc gatactcaag acgagtggaa acatctttct ctccaagaag
    33541 tagcggattt tgcagtagat aaactaaagc tgacagaaca aaaagacgaa cagctgagcc
    33601 aagctgtcat caacgaagct gagcaagcac atattcgatt tgttccgact gtcatcttgg
    33661 gtaaagaaat ctttgatgaa tctatttcaa tagaggaatt aaaagaattg atccaaacaa
    33721 aataaaaaaa taaggagcgt aggacaaaag taaagaacac ttttgtctca cgctctaaac
    33781 acctataaac ggcggaagca gaagcaactc cttcttattt cttggagtta aacgcttctg
    33841 tcccagcctc cttattccga ataaatggtg tccaaaagct tgcatctgcc agattatctc
    33901 atttcttatg acaagctgag caaattatct ccaccaaata atttattcat atttttgact
    33961 tgcgcatact aactaattct taaattttca tacaaaattt ctcaaattcc cttaacgagt
    34021 aatatttatg tacggtgatt cacactagta tcccaatcac tttttaagtt atcccgattt
    34081 caattctact tcaaaaatag ttgcttttat gtatgctgtt agttgattag atggtcgttt
    34141 gttgagctag gtcgtttatt ttaaacaagt tgtttcttta gcttatacct atctcgtcta
    34201 ccaaacgcaa gatggcaaac acaaatgcat acgcatctgt atttgccact ttgattcgta
    34261 taaagctaac aactcctaac tcctctcatt taatagttgc tctctttttt attcagatac
    34321 atttttgctg atcacagcct agtatttcaa tggttttgaa cactaaacca aattgttcca
    34381 ttacaacatt cactggctga tgaaatggat taatagattt cgattattca aagcgatttt
    34441 ctattggtgg attgtgttcc caactaacaa gacggattcc aaagtcatta tataactctt
    34501 caagagttaa ccctttcctg ttcccaatag atgtatctct cccacgctcg atataaaata
    34561 tggagtttcg aatgccaaga cctggcaaat ctttccttgt tacagttaca ctgctactaa
    34621 cttccccata taaaatccag ctcctccaaa atgataatat atttttcgac catcatctaa
    34681 ctcatgtaac aataattgtt gtgccctctt gtcactcgcc aaatgcaaac gccacgtgcg
    34741 ataccatgcc atgctacctg catctgcaga aaatccatca aataatgcaa caattgtatc
    34801 ttctatcaca atcctttccc catctacttc atattctaat ctaaaatcaa actcccctcg
    34861 ggtaatcgca ggtcttggtg gcttaggacc caatattatt ctacttaaaa ccgcattcgt
    34921 tcccagatga aacacagcta taacaacaat aatgattaat attttgtttc tatttgataa
    34981 atttctcatt attcgcaaat cacctctaca ttctcacata ctagttcgta atactccttt
    35041 aatatcaaaa tatctactga tcgtaataat attcgctatg aattcctact actcgtataa
    35101 gaaaggccaa atcatcagct tttacttgct gatgatttgg cttgtttcta tttatcgtaa
    35161 ttgttttcta atcaccaaaa ttgttaacaa tcggttcaga tatttcccaa ttcactagcc
    35221 ttatattata ttgatttaac aattcttctt catttattgt attttgatca agcggaccaa
    35281 attgttctaa taattctttg tcatgtaaaa tatcccttct agtaatgcct ctttcccttt
    35341 caatgaaaga agcactagga aaagtatgat tataaccatt caacctatct cccatgtaat
    35401 aattagcagg tcctggaaaa taaaatattt gcttattatc actaacttct aacagcaaca
    35461 ctaaatcttc tccgctggct aaacgacttg tccatctacg atatctccct atgccttcgt
    35521 tcattccaaa tccatcaaat tcaactatta acgtgtcttc tatcacatgt aattcttcat
    35581 tcaattcata ctcaagtcta aaaggaaatt ctccatatgt aatctctggc aatggtggtg
    35641 ctggagttct ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa
    35701 atgttatgag acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg
    35761 ctatatctag aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaaac
    35821 gcatttttgc cctcccttta taaacatcga ttctcaaatt ctaaaccaaa tcttggtagt
    35881 tcaagttcag gtataacgta ttccaaccat acatctcaat tactatttat gaacttatga
    35941 ttatcactga ataaatcact ccatcccctt gttaaaactt ctacattaat tacacctggt
    36001 tgttttcgac tagctaatcc aatgaactta tcaaccatcc ttatccacca accacgagaa
    36061 tcttgaccag aagtaagaag agcagttctc aaggatgatc gagcagttcc ttccagtttt
    36121 ttaattaatc aggccagtta tagataaata cttgatctta tctatatcag gacttctata
    36181 ttaagcaaaa atttatgtac aaaactaaaa tcttcattca gttgtctccc cgctttttat
    36241 acgattgtat taaaaacaat agaagccttt ccaaattatg ttactccagt aaagatggaa
    36301 ttatagtttg acggaagaaa agcaagtata acttttaaaa aaaactgata cattcgcatc
    36361 tcctgcttca tgtatcagtt ttttctttgg attaaactgc ttcttcacgc agtttttccg
    36421 caaattcatt tattttgcga attcggtgat taattcccga ttttgatatc gcacctgagg
    36481 gaatcatttc ccctaattct ttcaagctga cctctggatg ttgaagccga agttctgcga
    36541 tttcctgaag tttttcagga agggcatgta gaccgacctt tgcttcgatc aattcgatgt
    36601 tttcgatttg tttagaagcc gcatcaatcg tcttgttcag atttgctgtc tcacaattga
    36661 ctagacgatt gaccgaattt ctcatatccc gtacgattcg aacatcttca aatcggagca
    36721 ttgagttggt tgcaccaatc aatgttaaga aatcagcaat tttttctgct ccttttaaat
    36781 aagaaatata gccattgcgc cgttcaagcg ttcgagcatg tagaccgtaa taatttaaca
    36841 tatcgcaaat gtcttggtta tgctgttcat aaatagaaaa tatttccaaa tgataacggc
    36901 tcgtttccgg attattgact gaacctgagg ccataaaagc accacgtaag taagaacgca
    36961 ttttttgcga atttcccata atctcgtccg aaacgtgtcc attgaacatc ataccatcca
    37021 tgatatcaag atccatcaac acatcttttg tgccttgctt caaccggacg atatagacat
    37081 tgttcttttt taatttcatt tttttacgaa cgagtagctc acttcgtacc tgataatgat
    37141 ctttcaaaag agaatagata cgtctggcaa ttgcagcatt ttctgtttgg acgttcaaaa
    37201 taaactgctg gttcacaata cctaaagagc cgttcattcg cagcaaagca gctaattccg
    37261 ctttcgcatg ttctcgatga acttccaaac cagttagttc tttcttcaca tctgcagcaa
    37321 atgacatcct attctcctcc tttctccaac aataaattgc ttaatatttt gaaccgaaaa
    37381 ctaaacggaa caattcttct actactttgt ctccatcgtg gaagacacca ccatctttca
    37441 attccaaaaa gtcggtggaa atcactcgac atccttcatt tctcaatccg ttgaagtcgt
    37501 gtgcgacttg tactagatac tcatcataga tctctgggtc catataatct tcaggcactt
    37561 tttccgtatt gaccaatact gtatcgataa aaggctgatt caaatgcttg tgtaaaacac
    37621 gtatatgatc ggcatctgtg aaatgttccg tttccccttt ttgggtcatg atattacaga
    37681 tatagacggt ttcagcagtt gtcttgagca ttgcttcgcc gatttctggt atcactaaat
    37741 ttggaaggat actggtgaat aaacttccgg gtcccaaaac gaccatgtcg gcgtccataa
    37801 ttgctgaaac gacttttcga gccgctttgg cttcactttt atcatttgtg ttgcagacat
    37861 acacacggtc aatggtttta cggtcgatcg cgattttaga ttctcctact gcagtcgttc
    37921 cgtctttaaa gacagcatgc aaggtcaaag gagtttctga agaaggataa acatgtccat
    37981 ctacatgcat catttttgct aataattgaa tcgcctcata agtgcttccc cgcatctcgg
    38041 aaactgcggc aatgatcaga ttaccgatcg tatgattggc taaaaactga tcttcctttt
    38101 tgaaacggta ttggaaaata tcttcataga attgtggcat atccgataac gccactaaga
    38161 catttctcaa gtcaccagga ggagccatag cgatcgattc tctgattgca ccgctgcttc
    38221 ccccatcatc tgcaacagtc actactgctg tgatatctgc tccttgattt cgcaaacttt
    38281 tcaggataac tggcagacca gtgccaccgc cgaccactac gatctttggt tttcttatcc
    38341 ggtaggtttt cattttcacg accggttcac cgtctctttc cttttatcct tgtcccgatg
    38401 tgtcacattg actttatagt ttttccctaa agcatttgcc aaacgttcag tcaatgccac
    38461 agaacggtgt tgaccgcctg tacagccgat tgcaactgtc aagctgcttt ttccttcttt
    38521 aatgtatcca ggcaatacag tttccagaag atccaaaaat ttatggtaga atgattgcgt
    38581 ttcatcaaag gacatcacat aatcatagac tggctgatct tttccggtca atggtcttag
    38641 ttccgggata taatgagggt tgggcaaaaa acgcacatcc atcacaatat ccgaatcgat
    38701 cggcaacccg tatttaaaac caaatgatac aaattcgatc cgaaatcctt ggtctccctt
    38761 tgatttgaat gactcattga ttttttcacg taattgtcgt ggtgtcaatg tcgtcgtatc
    38821 aatgatcacg gaagcttggg ctttcaagtc gtctagtatc gcccgttctt tacgtatccc
    38881 ttcagtgatc aaaccgtcca tcgccattgg atgtgctcgt cttgtttctt tgtagcgagc
    38941 cactagttct tcgtctgaag catctaaaaa taagatagaa gtgtcgatga agttcgtatt
    39001 ttcgatttcg atcagcatgt tttggatttc ttcaaagaag gaacgagaac gtaaatccac
    39061 tcctaaagcc atttttgtga ctttgcctga ttctttgatt aattcccaga attttggaat
    39121 caatcgaggc ggcatattat cgatacaaaa atagcccata tcttcaaaac tttggatagc
    39181 tactgttttc cctgcaccgc tcatacctgt tatgatgact aattgcaaat tatctgtcat
    39241 aattacgaac tcctctcaca ttgtgcatag taaaattata tcattccggg cgtatcatta
    39301 ccagttttct ttttctttta caaaaagatt tggaccaaat atcttttagg atatttgccc
    39361 caaatccaat ttttttactt ttccgatgct gtatccaatg ccttgcttct ttcagctagc
    39421 gctcgttcaa tcgtccgatc tgctaaaata gattgggcat cgatcactgg ataatggtta
    39481 tcttcagcaa attcatgcat caatgacaat tctgtgcaac ctaagataac tttttcacag
    39541 tttaggcgct ctaccgcttc ttctaagatt tcgtaatata gctcttgatt caaataatcc
    39601 gattccttga tctcatgata gatcagataa ttgatttttt cttgaagaac agtatcagga
    39661 atcactgttt cgaatccaag atttttgact tcgcgttcat agattccagc tttcatactt
    39721 ccttctgttc ctagaatcgc tactctccct gtcgtatgct gacggacaag ttcatttgct
    39781 gcttctcttg gcatatgaag gataggaata tccgttgccg cttgtaattc ttcaaaaaag
    39841 tagtgtgcgg tgttacaagt caacacaatg aagttcggct gcaataaatt ttgtttctca
    39901 atatcatcca gcagaaacgg cattggattt tcttctgatc gatctaaaat ataagctgta
    39961 cgatcaggaa ctgttgcatg attgaataag acataattca aatattcttg atcttttgta
    40021 gctttcgttc ggtggttgat caagcgaaca aaactttctg tcgccatcgt gcccattccg
    40081 cctaaaatac tgaaaaaatt ctccatgaac tagccttctt tcacatgaaa atactttttg
    40141 aatcttggaa tatagttatg gaacatatat ttcatcaaca accagcgctt gattgaccgg
    40201 tctttttcat aaaacacagt agtaccgtaa cgattttctt tgatcatttg ctcagctagt
    40261 tttttgtctt cattttcccg agcatattct aaaaagattt ttttgggcac acccatccat
    40321 aatcttgctt tatctgattg attcgttcca taggtcgttt caacaaaggg tttgttaaac
    40381 actcgatctt ctgtgacgaa acgagccaga ttcaagccgt tcaatgtcac aaagaaactg
    40441 ctacgacctt gtctcaaatt gatttcaaat aatttatatt ctccatctct aggatcatac
    40501 ttcatatcga aattggcaaa gcctgtatat tcgatttttt ccaaaaatgc cttaatggtt
    40561 tgatagattt tttcgttata atctggcatg atcacaacgt aattaccaat cgatgctggt
    40621 gtaggatctt ctaacagtgg atgaccaaga cacatcatcc gaacttgatg atcttcatct
    40681 acatacgcat ttagcacacg catattacta tcatcacccg ggataaaatc ttgtacaatc
    40741 atttcacttt tataaccggc ttcgtatatc cggcctaaaa ttaaatcgaa ctcttctcta
    40801 gtctctaaaa taaaagcttt ttttcttcct tcaaattgga cagaaagata ctccacactg
    40861 tttgcaggtt tcaaagcaac tgggaattca aaaggcaatt cctgttccaa atgtccattt
    40921 actaacattt cttctctgac gatcaatgtt ttaggatacg gaagatcata ttcttcacaa
    40981 acttcataaa agcttacttt attgatcagt ctttcaaata aagaataatc aatatatgga
    41041 cagataaaat attctgacag ctcttgttta tgttgggaga tcaattctgc atatccatca
    41101 ccacaagcaa tcaaaagata ttttttactc ttatcagaat atctttcttt tgccaaacga
    41161 agcatcgtct cgataaacac agggtcttta tcaaatcctg gaataacttc tacattgaca
    41221 attttactgt agcgggtagg ggctaactga tccgaagcat atgcttggca gattttgccg
    41281 tatgcttcat taaaagaacg tgccatacca taaacgttca tgtcgcttcc taataatata
    41341 gggataaatt cttcattttc aatactgttc atcattatct ctcacttact cataatctct
    41401 tgtttttctt ttccaactac acaaggaata tcataccata aagaaaaaag caatcaaagg
    41461 aaactcctcc gattgctcaa ttcttttttg aacggattga acttatagga agtttttgtc
    41521 tataccaatc agtgtagaaa gaactaccat gtaatgattc tagaacactg atattaataa
    41581 aacaagcaaa aattcatttt tttatgtttt tttaactatc tcagctatct atcctcttta
    41641 taccattatt tttttcaaat agtgacctgt ataactttct tttacgttaa tgatttcttc
    41701 tggtgttcca gtcgcaagga tcgttcctcc accttctcca ccttctggtc ctaaatcaat
    41761 gatataatct gctgttttga ttacatctag attgtgttca atcactaaaa ctgtgttacc
    41821 agcatctact aatctttgta atacatgcaa caagcgggcg atgtcatcgc tatgaagtcc
    41881 tgtcgttggt tcatctagta tatagaaatt ctttccatta gagattttgt gcaattcact
    41941 ggcaagtttc atccgttgtg cctcaccacc ggacaatgtc gttgctggtt gccccatcgt
    42001 cacataacct aagccaacat caacaatcgt ttgcagtttg cgatgaatct ttggaatgtg
    42061 cttgaagaat tctacagcat cttctactgt catttccaaa atatcagcaa tgctttttcc
    42121 tttgtaatgc acttctaatg tttcagagtt atatcgtttg ccatgacata cttcacaagg
    42181 aacatagaca tcaggcaaaa agtgcatttc gatcttaata attccatccc cgcgacaagc
    42241 ttcacaacga ccgcctttta cgttgaaact aaaacgccct ttcttataac cccgcatttt
    42301 tgcctcgttc gtttgagcaa ataacccacg gatatcatca aatacacttg tataagtcgc
    42361 tggattactt ctcggcgtcc ggccgattgg gctttgatcg atatcgatga tcttttcgat
    42421 actttcgtag ccggaaattg tcttgaattt acctggctta gcagaattct tatttaattt
    42481 ttgcgctaac gatttcttta agatactatt gaccaatgta ctctttccag aaccagacac
    42541 acctgtgact gctacaaatt ctcctaatgg aaattcaaca ctaatatttt tcagattgtt
    42601 ttctgaagca ccagtgatct tgattgcttt cccattacct ttacggcgtt cttttggtac
    42661 agggattgat tttttccctg aaagatattt ccctgtcaaa gaattttcat cattagctac
    42721 ttcttcagga gtgcctgcag ccacgatttg tccgccttgt tctccggccc caggtccgac
    42781 gtcaatcaga taatctgctg cccacatcgt atcttcatca tgttcgacta caacgagggt
    42841 gttgccaaga tcacgcattt tctttaatga gccaatcagt cgatcgttgt cgcgttgatg
    42901 caatccgatc gatggttcat ctaaaatata caaaactcca gaaagattcg atccaatttg
    42961 tgtagccaat ctgattcgct gcgcttctcc cccagataat gttccggaag cgcgactcaa
    43021 agttagatag tctagtccca cattttccaa gaatgaaagt ctgtcattta cttcttttaa
    43081 aatcggtttg gcaattactt tctcttgttc agataacgtt aagtttgaga agaaatctac
    43141 tgctttttga atagacaact catttgtttg cccgatattc gttccattga tttgaacaga
    43201 tagagcttgg ggattcaatc gataaccttt acatgtctga caagttaatt ctgtcatata
    43261 aagacgcatt tgctcacgcg tgaaatcact gttcgtatca tgataacgac gctcgatatt
    43321 cttcaatacc ccttcgaaag tcgtttctac atcgcgaacg ccaccaaaat cattttcata
    43381 atggaaatgg aaaagatcat ctgtcccatt caagatgata tcctgatgct cttccggtaa
    43441 ttcttcgaat ggtgtatcta aatcgatacc gaaactttca caagcctgcg ctaacatttg
    43501 aggataatac tgagagctga ttggattcca tggaacgata gcaccttgac taagtgtctt
    43561 cgttctatct ggaatcacta aatcttgatc gacttctaat ttgacaccta aaccatcaca
    43621 gtctggacaa gctccaaaag gcgcattgaa tgagaacagt cttggttcta gttcgccaac
    43681 agtaaatccg caataaggac aggcatagtg ttcgctaaac aacatttctt cttcaccgat
    43741 cacgtcaacc agagcatagc cgtctgccaa tcgcagagcc gcctctaaag aatcaaatag
    43801 acgagaacga atcccttctt tcacaatgat ccggtcgatg acgatcgcga tatcatgttt
    43861 tttgtttttc tccagctcag gcacttcact taaatcatag atctcaccat cgactcgaac
    43921 gcggacgtat ccctcttttt ggattcgttc gaatattttt ttatgttgtc cttttttctt
    43981 taccacgatc ggtgccaaca cttggatctt tgtgcgctca ggcaaagcca ttacttgatc
    44041 tgccatttga tctactgatt gactggtaat ctcgatatga tcgttgggac aaatcggatg
    44101 tcctacacgt gcaaacaaca agcgtaaata gtcattgatt tccgtcacag tacctactgt
    44161 cgaccgagga tttttacttg ttgttttctg atcaatagaa atcgccggac tcaatccgtc
    44221 gatgctatct acgtccggct tatccatttg tcctaaaaac tgacgagcat aagcagaaag
    44281 gctctctacg tatctccgct gcccttcagc gtacagcgta tcaaatgcta gggaactttt
    44341 tccagaaccg gacaaacctg taacgaccac gaatttatct cgtggaatcg ttacatctat
    44401 atttttcaag ttatgggcac gtgcaccgtg aatcgtaatt ttatcgttag ccatgttgtc
    44461 ctcctacttt gaagctttta attctaaaat catatctcgc acattagcgg ctgtttcaaa
    44521 atccaatgct ttcgccgcat ttttcatttc tttttctaat ttcatcaata acgtatcttt
    44581 ttcttctttc gtcatttctt catacgagac ttgtaccgct tcttttgtat catcttccga
    44641 ttctttcgta atagaaatca aatctcggat ttctttaatg atcgtttttg gaacgatccc
    44701 atgttcttca ttgtacttct gctgaatcgt acggcgtctt gatgtttcat ccatcgctag
    44761 acgcatagaa tctgttactt tgtctgcgta catgattact ttaccttctt cattccgagc
    44821 agctcgtcca atcgtttgga ctaaggaacg ttcactacgt aaaaagcctt ctttatccgc
    44881 atccaagata gcaacaaggg aaacttcagg tacgtctaat ccttcacgaa gcaaattgat
    44941 tccaactaac acatcaaatt cacctaatcg aagatctcga atgatttctg ttcgttccag
    45001 cgttttgata tcactatgca aatatttgac tttgatccct aattctttga aataatctgt
    45061 gaggtcttct gccatttttt tcgtcaatgt cgttacaaac acccgctggt ctttttcgac
    45121 tcgttcattt atttcgccta ctagatcatc gatttgtccc atgattggac gaacttcgat
    45181 cactgggtca agcagccctg tcggacggat gatttgttgg ataacggtat ctgtttgttc
    45241 atgttcatat ggtcctggcg tcgcagagac ataaatgatc tgattgacat gtttttcaaa
    45301 ctcttctaac cgaagtggcc gattgtctaa agcagatggc aaacggaaac cgtaatctac
    45361 tagcatctgt tttctcgctc ggtcaccatt atacattcct ctgacttgag gcatggttac
    45421 gtgcgattcg tccgccacga tcaaaaaatc ttcaggaaag aaatccaata gcgtataagg
    45481 cggttcacct tctttacgtc cgtccatatg ccgagaataa ttttcaattc ccgacgtgta
    45541 gcccatttct aacatcattt caatatcgta attggttcgc tgttctaaac gttgagcttc
    45601 cagaagtttg ttttcatttc gcaagacagt taaacgctgt tctaattccg ctttgatatt
    45661 cgctacggca tgctccatat gttcatcatt cgtcacaaag tgagtcgccg ggaagattgc
    45721 cacgtgttcc gtttctccta aaacttcacc tgtcagtgca ttgacttctc ggatacgttc
    45781 gacctcatct ccaaaaaact ccacacgtaa ggcacgttca tctcttgaag ccggaaaaat
    45841 ctccacgaca tcaccgcgca ctcgaaaacg accgcgctga aaatctatat catttctttc
    45901 gaattggata ctgactaagt cacgaatcaa ctgattgcga tctagttctg ctccctgtcg
    45961 aatcgaaact acttgttttt ggtactcaaa aggcgaacct aatccaaaaa tacatgaaac
    46021 ggatgcaatg acgatcacat catttctttc taataatgaa ctagtagccg aatgtcgtaa
    46081 tttgtcgatc tcatcattta cacttgaatc tttttcgata tacgtatcgc ttgaaggcac
    46141 ataagcttca ggctgataat aatcataata agaaacaaaa tattcaacag cattgttagg
    46201 gaaaaattcc ttgaattccc cgtacagctg accagccaat gttttgttat gggcaataat
    46261 tagtgttggt ttattcactt tctcaataag attggaaatc gtatatgttt tccctgttcc
    46321 agtcgcacct aataagattt gtgctttttt cccgccgact acgccatcca ctaattggtt
    46381 aatcgcttca ggctgatcac cagccggttg gtatttcgag acaagctcaa agtgtcggga
    46441 ggtatctctc tcaatcattc ttcgtcctcc ttttcattca ttccttttca ttttacgacc
    46501 aataaaaaga aaaggaaagc aaaggcattc cttactaaaa aattatacca caccgaacat
    46561 actttcgcca cttttttatc ttattatgca tgttacgttt tataacataa aatgttagaa
    46621 actcgcattt ttttcatttt ttcgagctta cagtcttgtc tgattcagca cttttcgcct
    46681 ataataataa aatattggaa atttaggagg ttgattatga aaagaaagca tttatttcac
    46741 tcatttgttt tcttaatgac aggacttgtg acacttgcgt ttggacatgt tgcacaagct
    46801 gacaacacta caacggcaga gtcaagcaca agcgagacaa tgcaaaaaat agagcccaaa
    46861 aaagacgtat acgtaattgc cagtgactct gcttttgctc catttgaatt tcaaaacgca
    46921 aagggcgagt acgaaggcat cgatgtaaaa ttgatggaaa acatcgccaa actgcaagga
    46981 tttaacatcc gaatggacta ccgtggtttc tctgctgctt tgcaagcttt ggaatcagga
    47041 caagcagacg gaatgattgc cggaatgact gtaacagatg aacgaaagaa aagctatgat
    47101 ttttctgatt cttactttga tagcgggatc caattagctg tcaaaaaagg agatacttcg
    47161 atcaagtctt atgaagattt gaaaggaaaa acagtcggag caaaagttgg taccgaaagt
    47221 gctgaatttt taagcgacca tgaatctgaa tatggctatt ctatcaaata ttttgatgct
    47281 gctgatcaat tgtacgacgg gttaaaagtt ggcgcaatcg atgcaatgat ggatgattat
    47341 ccggtaatcg gttatgcgat caaacaagga caagaattgg aaacaccgat caaaaaagaa
    47401 tctggcggaa aatatgcatt tgccgttaaa aaaggacaaa atcctgagct tcttaatatg
    47461 ttcaatgaag gattgaaaga attaaaaaga acaggcgaat acgataagat tattaaagaa
    47521 tatgtagcgg atggttcaga aacttccagt gagtcttcca ctgctgatga atcaacattt
    47581 cttggactta tccaaaacaa ctatcaagca ttgttgaaag gactttggag aacaattctc
    47641 ctaactttga tctcctttgc tttggctata gtaatcggtg tgatttttgg attattcagt
    47701 gtagcaccaa tacgcgctct tcgcacaatt gctagtattt atgtcgatat catccgagga
    47761 attccaatga tggttcttgc gttctttatt ttctttgggt tgcctggtat cgttggattt
    47821 acgattcctg acttccttgc tggtgttatc accttgactc ttaacgcaag tgcttatatc
    47881 gcagagattg ttcgaggcgg gatcaacgcc gttcctgtcg gacaaatgga agcttccaga
    47941 agtttaggtc tttcttataa tcggacaatg caaaaaatca ttttgccgca agcgatacgc
    48001 atcatgatcc catcattcgt caatcagttc gttatctcat taaaagacac aacgatcatt
    48061 tccgcaatcg gtgtagtcga attgctgcaa actggtaaga tcattgttgc acgtacaaca
    48121 caaagtacgt atgtttactt gatcattgcg atcatgtact tgattttgat cacagcattg
    48181 acaaaattag caaaagtttt agaaaagaag gtgaagtaag atggcagaaa aaatcttagt
    48241 tgagcattta gtcaaaaaat acggcgataa cacagtatta aacgacatca atgtgtccat
    48301 caacgaagga gatgtcgtct gtatcattgg cccttcaggt tcaggaaaaa gtacattttt
    48361 gcgttgcttg aatcgtttag aagaaccttc tgacggtgaa attattattg acggcgccca
    48421 cttgatggat aaaagcacag atatcaactt ggtgagacaa catatcggaa tggtattcca
    48481 gcactttaac ctattccctc atctttctat tttggaaaac atcattttag caccgatgga
    48541 cctgaagaaa gaatctagag aagaagcaga gaaaaaagca atcaaattgc tgcagacagt
    48601 tggtctagaa gataaaaaag atatgtatcc tgaaatgctt tccggtggac aaaagcagcg
    48661 tgtggctatt gctcgtgctc tagcgatgaa ccctgacatc atgctttttg atgagcctac
    48721 atctgcactc gaccccgaaa tggtcggtga tgtcttgaac gtcatgaaaa agttagcaga
    48781 acaaggtatg acgatggtta tcgttacgca cgaaatgggc ttcgctaaag aagtggccaa
    48841 tcgtgtcatg ttcattgacg acggaaactt tttagaagac ggaacgcctc aacaaatctt
    48901 tgaaaatccg caaaatgaac ggacaaaaga tttcttgaat aaagtattaa atatttaata
    48961 aaaaccaaaa atgcccggtc gcttcatgac gaccgggcat ttttagtttc ttgttttttc
    49021 acttaactgt accagccgca tcgcttgttc tcctgcacaa acaagcaatc gctctacctg
    49081 ttccatcgca taagaaagta ccatcggttc attgacgata tcaagtacat agtcgatacc
    49141 accttcagaa actatgatga ttatcttatt ctaaagctgg cttttttgtt ggtacaaaca
    49201 tgacaactac aaaaaaacca ggcgaacgta tgattgttcg tctggttttt aattattaat
    49261 gacttagcgt ttttactaga aatagaaaat ttgggcaagt ctactttata tttttactag
    49321 aaaaactact tccgcttttt cccgccaaaa gcatctttaa acttatcgaa gaaaccgtct
    49381 tcttgctgtt cttcaacttt gatgccgcca gcttcagcga atgcacgtaa tgcttctctt
    49441 tgttgttcat ttagattttt cggtgtgatc agttttactt tgacatgttg gtcgcctgtt
    49501 gcaccaccac gtaacttcgg agcgcctttt ccacgtaaac ggaagttcgt acctgtctgt
    49561 gttccagcag ggatcttcaa tttcacatct ccatggacag ttggtacttg gacttcatcc
    49621 cccaaagctg cttgtacaaa acttaaaggc aattcatagt aaatttcaga accttcacga
    49681 tcaaagatat cactttcttc tacacgaaaa accacataca aatcaccaaa tggtccgccg
    49741 ttttctccag cttctccttg accagctaaa cgcatttgtt gtccttcttc tacaccagct
    49801 ggtacattca ctttaactga atgtgctttc ttctcatgtc ctgagccatg acaggttgga
    49861 caaggttctt tgatttcttt ccctgtgcca tgacaaacat cacaagtttg acggctcatc
    49921 actcgtccaa gtggcgtttg gcgttctaca ttgatcgtac ctgcaccatg acatttatga
    49981 caagtagttg gttgtgtgcc tggttttgca ccgtttccac cacaagtatg acagatttct
    50041 tcccgattat acttgatttc tttttctaca ccaaaaatcg cttcttcaaa agacaagtcg
    50101 accgtatatt gcaaatcagc accttgacga ggcgcatttg gatcgactga ccgaccgcct
    50161 cctccaaaga aagagtcgaa gatatcttca aaaccaccga agccgccacc agagaagccg
    50221 ccgaatccac caaatccgcc accagctcca ccagcaccgt aatttggatc tgtgcctgca
    50281 tgcccgtatt ggtcgtatgc agcgcgtttt tgcgggtcac ttaaaatctc atatgcttct
    50341 gatacttctt tgaatttttc ttccgcatca ggttctttat tgatgtcagg atggtatttt
    50401 ttcgaaagct tacgataagc tttctttatt tcatcttctg atgctccttt tgagagccct
    50461 aagacttcat aataatcacg ttttgttgcc ataggctttc ctcctattgg ttctcatcat
    50521 tttcacgtcg cttgttatca tatcatattt tgtttgaaac aaaaaccgat acttcaaact
    50581 atttttcaaa agaaaaagcc aaagtagcaa ctttggcctt ctctcgaaat tcagtgaact
    50641 gattatttat cgtcgccatc gacttcttca aaatcagcat caacgacatc atctgctccg
    50701 ccttgagctg cttctggatt ttcttgagct tgttgttgtg cagcttgttc gtatagttta
    50761 acagtcaagt tttgaacgat ttcgttcaat gcatcgcgtt tagttttcat ttcctcgatg
    50821 ttgtttgctt caacagcggc ttttaattcg tcacgagctg tttctgcttt tttcacttct
    50881 tcttcgtcca ctttgccttc taattctttc aatgttttgt ccacagagaa tagcaatgcg
    50941 tctacatcat tacgtaggtc aacttcctct ttacgagctt tatctgcttc agcgtttgct
    51001 tctgcatctt tcaccatacg ttcgatttct tcgtctgtta gacctgaaga agatttgatc
    51061 gtgatttttt gttctttttg tgtacctaga tctttcgcag atacatttac aatcccgttt
    51121 ttatcaatat cgaatgttac ttcgatttgt ggaataccac gaggagctgc tggaatatct
    51181 gtcaattgga atcttcctag agttttgttg tcagcagcca ttgggcgttc accttgtaat
    51241 acatggatgt ctactgctgg ttgattatct gccgcagttg agaatacttg tgatttactt
    51301 gttggaatcg ttgtattgcg gtcaatcaat ttagtgaaca ctccacccat tgtttcgata
    51361 cctaatgaca atggtgtaac gtcaagtaaa acaacatctt tcacatcacc agtgatcacg
    51421 ccaccttgga tagcagcacc catagcaact acttcatcag ggttaactga tttgtttggt
    51481 tcttttcctg tttctttacg tactgcttct acaactgcag ggatacgagt agatccaccg
    51541 actaaaataa cttcgtcgat ttctgattgt gacagacctg catcttttaa cgcttgacgt
    51601 actggtactt ttgtacgttc tactaagtca gctgttaatt catcaaattt tgcacgtgtc
    51661 aatgtcattt ccaaatgcaa tggtccagat tctcctgctg tgataaatgg caagctgatt
    51721 tgtgtgcttg atacgccaga taaatctttt ttcgcttttt cagcagcgtc tttcaagcgt
    51781 tgtaaagcca ttttgtcttg agacaagtca atgctgtttt cttttttgaa ttctgctacc
    51841 atgtagtcga tgattttgtt atcaaagtcg tccccaccta gatggttgtc gccagctgtt
    51901 gacaatacat caaagacacc gtctcctagt tcaaggatag atacgtcaaa agtaccgcca
    51961 ccaaggtcga atactaagat tttttcgtcg cgatctgttt tatctaaacc ataggcaaga
    52021 gcagctgcag ttggttcatt gacgatacgt tctacttcta gaccagcgat cttaccagca
    52081 tcttttgttg cttgacgttg tgcatcatta aagtaagctg gcacagtgat aactgctttt
    52141 tcgacttttt cacctaaata gtcttctgcg aatcctttga tatattgaag gatcattgct
    52201 gagatttctt gtggtgtata tgatttacct tctacatcta ctttgtaacc tgcttcaccg
    52261 atatggcgtt tgattgatga aatcgtattt gggtttgtca cagcttgacg ttttgcaact
    52321 tcccctactt ggatttctcc atttttgaat gatacaacag aaggtgttgt acggtttcct
    52381 tctgggttag cgatgatttt tgcttcgttt ccttccaata ctgctactgc agagttagtc
    52441 gtacctaagt caataccgat aattttactc ataattaacg tctccttctt taattgaaag
    52501 tgtaagtttt atatccgcac aattattgtg caacaatgac catagttggt cttaacacgc
    52561 gatcatgtaa tttgtagcct tcttgtagta cttctacaat cgtatcggct ggtgtatctt
    52621 cagtcgctgg aacagtttgt actgcttgat gaagattagg atcaaaggct tctcctttag
    52681 cagggatttt ttcaattcct tcttcttcca gtgcgttacg caaactttcc aagaccattt
    52741 ccacgccttt tttcaaacct gccccttgat catctgacac ttctgtggct aatgcacgtt
    52801 ccaggttgtc aattgatgga agtaattttt tggctaaatc ttgtgaacgg tatttttgta
    52861 attgctcacg ctcatttttt cctcgatttg ccatatttgc gatttcagca cgagcccgca
    52921 agtatttatc ttccatttct tctaactcag cttttaattt cgctgtttcg aattcttccg
    52981 cttcagcttc tgaaataccc gaatcttctg cagcttcgac atcgatttcc ggttctggct
    53041 gagcatctgt catttcttta tctagttctt cttgattttc tttcacgata cagtcttcct
    53101 ttcattattc agtcagctgt ttgattgctc ttactaagaa agagagaacg atcagctgca
    53161 aatagcttcg ttattcacat tacgagtttg acaagtcaag cgagcggtag taatcagcaa
    53221 gtttagctgc tagttctctt cgataaacat cgactagacc gaacatccga gaataaggca
    53281 tactagtagg ccctaacaag gctattgtac ctcttccatg accatctatc tcgtaacttg
    53341 cttgaatcat actcatattc tgcaataaat cattgcctaa ttcagcacca atcttgatat
    53401 gaatattgct atccatcgga gtgatcattt gtgtaagtcc gtctgagtct ttcataaaag
    53461 aaaacatcga tttgaactga tcaagatctt gacggggctc ataatccaag agattcattt
    53521 gtccactgac ataaaccttt tcttcaaaaa catgaccaag aacggtgtca aataactgag
    53581 acatcccttc tgtcgtctgg aaatatttat gaagaatcat cggaatctcc gtacgaagtt
    53641 tatggtacac cgtcatcaga ggatctccga ctagacggtc gtttacaatc tgtaccattt
    53701 tttctagttc ctgacttccc atatattcgg gaagagtgaa cacttggctt tctacattcc
    53761 ccatatctgt tacgacgata gcaataatct gtcgattatt caagggtacg atacgaaagc
    53821 cagtcagtcg gcggtctttt atttcaggtc ccaatgaaaa agtcgtatag cttgtcagct
    53881 cagataatat ctcagcagat tgcttaataa tctcattgat ttcatgaaat tctctgccta
    53941 gagactgacg aatcagcagc aaatcatctt ctgctacgcg agcaggtttt aacaaatgat
    54001 ccacgtaata acgatagcct aaagcagatg gaatccgccc agaagaagag tgagtcttca
    54061 acagcaatcc ttcatcttct aatgctttca tatcattgcg aatcgttgct gagctggcct
    54121 tgactccctc agacattaat gttttggaac caacgggcac gccactactc gtatagtgtt
    54181 ggatgatcag acgtaaaatg tcgttttgtc gctttgttaa catcttcctc acctctcatt
    54241 agcactcata tattctaagt gctaattcac ttataaatat agcaagaagc ccttctcctg
    54301 tcaagagaaa gactccattt tttagcactc taaacacaag agtgctaatt ctaaataatt
    54361 gatgtaaatc cagttatatc aatacttcaa tacctcattg tcaaactttc taaacttttt
    54421 cttttcaagt ttcgataaaa aacaaatatt cttcaaacta tttttcaaat aaaaagcgct
    54481 caaacacatt gttccccaaa aaagtccctc ttggtgtcaa ataaacacgg tcttcttcat
    54541 taacaagtaa cccttcttgc tctaattccc ttagcacatc accgtacacc gattcaattg
    54601 ttttttgata gcgatcatag aaatgttttt tagtaatccc tgtttttttt cttagtccta
    54661 agaacatttc ttcttctatt tgattttttc gtgtcaattt ttcttcttcc aataccgggc
    54721 gttcatttgc tcgtaatggg cgtaaatagt gttggattgg tcctttgttg cggtaacgaa
    54781 tatctccaag ataaccgcta gcaccagctc caaacccaaa atagtggtca ttgttccagt
    54841 acatgaggtt atgttgagat tcatgtccgg agagcccaaa gttacttatt tcatattggt
    54901 gacgtccatg cttttccatt gcttcaatcg tctcttcaaa cattctggtt tccgtttcct
    54961 gatcaggcag ttcgagtctt ccttgtcgta cccagttcat gaacattgtt ttgttttcga
    55021 ggatcaatga gtacaaagaa tagtgaggca gatccaaagc caaagctttt tccaaagtat
    55081 cccgatatcc ttctagtgtt tgtcccggta atgcataaat caaatcgatg cttacattgg
    55141 taaaattttc tgcttctaaa aattgcattg tttgatacac atcttctgca gtatgtttac
    55201 gtccaatttt tttcaataag cgattgtcaa atgtctgcac tcccatggat aaacgattga
    55261 cgccatagtt tttcatcact tgaagttttt cacgggtcaa atcaccagga ttcgcttcta
    55321 cagtaaactc attaccattt tgaaaaggca ggatctctct agcacctgat aatagtcgat
    55381 caagttgttt agcagataaa gaagttggtg ttccgcctcc tacataaagt gtttccaaat
    55441 tcgctgtagg atgactttcc agcattaatc tcatttcatt cagcagcatt tccacatact
    55501 catctactgg ctgcccttct aaaaaaactt tattaaagtc acagtaatag cagatatgct
    55561 cgcaaaatgg gatatgaatg tacgcagaaa ccttgttttc tgcgggattt attgtttcta
    55621 tcattgaaat tctttctcct tacgtcgaaa agtcgtgcct gttttcgacc ttcttaaaat
    55681 cgcgttcaag caccaactca ttgaaataag ccgcatattt agaatcaagg aaacctagct
    55741 ctaaaaatgc ttgcttattg tcatcggagc tacacggtgc ttttacagcc tctcgataaa
    55801 cggtaatcgc aaagctgacc ttcggcatta agtcaaaatc tgcaaaaata tgaaaagcta
    55861 ttttcacaga ttcctcctta agtgctcaga ccacaacgct ttgcttacaa cctctcatat
    55921 acgcatacta aacgtatttg gcgaacaaat ccactaaaaa atccaagaat cgacttcttg
    55981 gatgattttt ttacactcat gattcttcct aaacaatttt gtccttattt tattcataaa
    56041 aaaatctagt caaatctctc acttcaagag ttttaactag attccaatta ttattttttt
    56101 gtaaaaataa acagtctctt aatcatagac atttcttcgg tgccctactt gaatagcgat
    56161 aacaacgaat tgattatctt ctatctctac caatacacgt atttgccaat cagagctgct
    56221 tctgaatttc gggcgtgaat tttaaacggc aggtcattcc agcccaccaa attcattaag
    56281 gattcttcca tgaatactgt tgttttccca cttcttatcc aatgtttatg ggcaatttca
    56341 gccgtttgac gattgtattc atcttctagt tgttccatcg aatagttttt tagaagttca
    56401 gataaagtaa tcccataaaa ttcagccata taagaaagcc attctttttt ggcgtcactt
    56461 acacgaaccg taatcatagc cctataattg cctctcttaa agaatctcat gcatttcctg
    56521 atgtgatttt agaaaaattt acttgctaaa aaactgtgaa aatgtaggag ctagcatcct
    56581 tgtagtaaat gatcgagctc tttattatac attaaaaaag ttcagaaatt ttgatgaagt
    56641 agccaactac tttttcatga gtaaagcaac actagaaaaa gaatcctcca gtacttagaa
    56701 tttactggag gatgtacttt tgaagaagcc tataggttat ttaaaaaata ttactacagg
    56761 aaggaacgag aattacttcg ttacttataa tagaacttag tcttatatag aaggcatagc
    56821 tcacattagc ttatttgtcc tataaaaata aattacggta ttttagaaaa gctattttct
    56881 cttttttcaa cgatcttcag caatttttct ttatcgtcag gtaactcaag ttcatgaatt
    56941 ctagcatgac aattgggaca taacgcgata cagttttcaa tcacatcttt tccaccttgc
    57001 gacaaatatt ctatatgatg acaatgtaaa taaggtacac cgtcttttat aaatggccct
    57061 ttttcttggc aaagttgaca aatacctttt gcaagtcgtt taacatattc agatacgaag
    57121 ggattacgtt tatatatttt tgtttttgag aagcaaaact cctcttcgtt aacatatttt
    57181 gcagccattt ttttattata attactaact tttcttgctt cttcctctaa tttagcatta
    57241 gataaattac gaatatgtct agtttgattt tcttcctggt tatatatctt tatcttcaat
    57301 caattgctct tgatttatta aagctaatgg aaatttatat acttttctgc tgttgcccga
    57361 actatctttt tcattaacag tatatatttg tcctgctaaa tatacttctc ccagatatgt
    57421 gtgttctttt ggtttaaatg tttcaaataa atgaatcttt acaaaattac tttttgaatt
    57481 ggctaaaact ttattttgat tcttatccaa tacttgatcc ccttttagcc ccataccggt
    57541 atattgtaaa ataccattta accacttatc tttataaaca ttacttcctg tttgatcact
    57601 aaacagtaca agagtattag tttgatgaga tcttctcatt ccaccctgtt tggaacattt
    57661 aaacttctct gaaatttctt tgttagtata tactctacca acttctaact cacctaatat
    57721 gtttttagac caattttctc gccatgtaat aatcttatca agattaaaca tttctccctc
    57781 ttttggcatg ccatttctaa ttaaattagc taatacacca gtagaaatat ctaatttttt
    57841 tagtaattct ttcttagtaa tcaatgttgg attcataaca atgcagctcc taatgatatc
    57901 aatataattg cattagataa cttgaaaaat cgaaaaaaac tcattttgtg tcaagttatc
    57961 tgtaatatat aaaaatatca ttattatacc tcatctatgt gaatttttac tattcttacc
    58021 ttagcatatt ctttataggt atatcagagc taatcaaact aaaaaatacc cgaagtcttc
    58081 ttatgtaaaa acaccggtta ttacttaaat atgacttagg taaaacaatg agtgatccaa
    58141 ttataaaatt ttatggcgaa taatttagta agcgctacat attgaatgat ttagaaattt
    58201 tgaaaaagta gctagttcct tttataccag aaaagtaaac ctaataaaaa acacaactaa
    58261 tttttagctg tgttt
//
LOCUS       DACCQI010000002        54287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCQI010000002 DACCQI010000000
VERSION     DACCQI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.002"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IVO76_000046"
     CDS             complement(543..2048)
                     /locus_tag="IVO76_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7428042.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IVO76_000047"
     CDS             complement(2045..2731)
                     /locus_tag="IVO76_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7428043.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVO76_000048"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVO76_000048"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7428044.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IVO76_000049"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IVO76_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7428045.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IVO76_000050"
     CDS             complement(4752..5321)
                     /locus_tag="IVO76_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7428046.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IVO76_000051"
     CDS             5507..6409
                     /locus_tag="IVO76_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7428047.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IVO76_000052"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IVO76_000052"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7428048.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IVO76_000053"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IVO76_000053"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7428049.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IVO76_000054"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IVO76_000054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7428050.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IVO76_000055"
     CDS             12977..13162
                     /locus_tag="IVO76_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7428051.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IVO76_000056"
     CDS             complement(13224..14420)
                     /locus_tag="IVO76_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7428052.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IVO76_000057"
     CDS             complement(14590..16026)
                     /locus_tag="IVO76_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7428053.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IVO76_000058"
     CDS             16319..17128
                     /locus_tag="IVO76_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7428054.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(17314..19467)
                     /locus_tag="IVO76_000059"
     CDS             complement(17314..19467)
                     /locus_tag="IVO76_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7428055.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(19761..20609)
                     /locus_tag="IVO76_000060"
     CDS             complement(19761..20609)
                     /locus_tag="IVO76_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7428056.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(20833..22770)
                     /gene="thrS"
                     /locus_tag="IVO76_000061"
     CDS             complement(20833..22770)
                     /gene="thrS"
                     /locus_tag="IVO76_000061"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7428057.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYGT
                     AFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTIE
                     RYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMNC
                     PHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPDQ
                     IKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAMD
                     ELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGEN
                     THRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIKE
                     RLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETAV
                     EDLNIFIDAMEAEVKNYSREN"
     gene            complement(23107..23286)
                     /locus_tag="IVO76_000062"
     CDS             complement(23107..23286)
                     /locus_tag="IVO76_000062"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7428058.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(23333..25156)
                     /gene="cadA"
                     /locus_tag="IVO76_000063"
     CDS             complement(23333..25156)
                     /gene="cadA"
                     /locus_tag="IVO76_000063"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7428059.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            25434..27713
                     /locus_tag="IVO76_000064"
     CDS             25434..27713
                     /locus_tag="IVO76_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7428060.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(27806..28453)
                     /locus_tag="IVO76_000065"
     CDS             complement(27806..28453)
                     /locus_tag="IVO76_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428061.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(28632..29351)
                     /locus_tag="IVO76_000066"
     CDS             complement(28632..29351)
                     /locus_tag="IVO76_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7428062.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(29482..30633)
                     /locus_tag="IVO76_000067"
     CDS             complement(29482..30633)
                     /locus_tag="IVO76_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7428063.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(30850..31590)
                     /locus_tag="IVO76_000068"
     CDS             complement(30850..31590)
                     /locus_tag="IVO76_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7428064.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(31594..31917)
                     /gene="rsfS"
                     /locus_tag="IVO76_000069"
     CDS             complement(31594..31917)
                     /gene="rsfS"
                     /locus_tag="IVO76_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7428065.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(31974..32567)
                     /locus_tag="IVO76_000070"
     CDS             complement(31974..32567)
                     /locus_tag="IVO76_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7428066.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(32551..33198)
                     /locus_tag="IVO76_000071"
     CDS             complement(32551..33198)
                     /locus_tag="IVO76_000071"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7428067.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(33211..33522)
                     /gene="yhbY"
                     /locus_tag="IVO76_000072"
     CDS             complement(33211..33522)
                     /gene="yhbY"
                     /locus_tag="IVO76_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7428068.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(33538..34647)
                     /gene="yqeH"
                     /locus_tag="IVO76_000073"
     CDS             complement(33538..34647)
                     /gene="yqeH"
                     /locus_tag="IVO76_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7428069.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(34644..35171)
                     /locus_tag="IVO76_000074"
     CDS             complement(34644..35171)
                     /locus_tag="IVO76_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7428070.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(35351..36136)
                     /locus_tag="IVO76_000075"
     CDS             complement(35351..36136)
                     /locus_tag="IVO76_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7428071.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(36129..36998)
                     /locus_tag="IVO76_000076"
     CDS             complement(36129..36998)
                     /locus_tag="IVO76_000076"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7428072.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(36999..38378)
                     /locus_tag="IVO76_000077"
     CDS             complement(36999..38378)
                     /locus_tag="IVO76_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7428073.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(38386..38805)
                     /gene="fabZ"
                     /locus_tag="IVO76_000078"
     CDS             complement(38386..38805)
                     /gene="fabZ"
                     /locus_tag="IVO76_000078"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7428074.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(38830..39306)
                     /gene="accB"
                     /locus_tag="IVO76_000079"
     CDS             complement(38830..39306)
                     /gene="accB"
                     /locus_tag="IVO76_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7428075.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(39309..40544)
                     /gene="fabF"
                     /locus_tag="IVO76_000080"
     CDS             complement(39309..40544)
                     /gene="fabF"
                     /locus_tag="IVO76_000080"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7428076.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAIS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(40563..41300)
                     /gene="fabG"
                     /locus_tag="IVO76_000081"
     CDS             complement(40563..41300)
                     /gene="fabG"
                     /locus_tag="IVO76_000081"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7428077.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(41312..42229)
                     /gene="fabD"
                     /locus_tag="IVO76_000082"
     CDS             complement(41312..42229)
                     /gene="fabD"
                     /locus_tag="IVO76_000082"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7428078.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(42336..42560)
                     /locus_tag="IVO76_000083"
     CDS             complement(42336..42560)
                     /locus_tag="IVO76_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7428079.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(42613..43575)
                     /locus_tag="IVO76_000084"
     CDS             complement(42613..43575)
                     /locus_tag="IVO76_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7428080.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(43585..44025)
                     /locus_tag="IVO76_000085"
     CDS             complement(43585..44025)
                     /locus_tag="IVO76_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7428081.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            44331..45296
                     /locus_tag="IVO76_000086"
     CDS             44331..45296
                     /locus_tag="IVO76_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7428082.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(45353..45583)
                     /locus_tag="IVO76_000087"
     CDS             complement(45353..45583)
                     /locus_tag="IVO76_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7428083.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(45669..46556)
                     /locus_tag="IVO76_000088"
     CDS             complement(45669..46556)
                     /locus_tag="IVO76_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7428084.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(46645..47868)
                     /locus_tag="IVO76_000089"
     CDS             complement(46645..47868)
                     /locus_tag="IVO76_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7428085.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(47885..48964)
                     /locus_tag="IVO76_000090"
     CDS             complement(47885..48964)
                     /locus_tag="IVO76_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7428086.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(49095..50822)
                     /gene="ptsP"
                     /locus_tag="IVO76_000091"
     CDS             complement(49095..50822)
                     /gene="ptsP"
                     /locus_tag="IVO76_000091"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7428087.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(50822..51088)
                     /locus_tag="IVO76_000092"
     CDS             complement(50822..51088)
                     /locus_tag="IVO76_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7428088.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            51567..53801
                     /locus_tag="IVO76_000093"
     CDS             51567..53801
                     /locus_tag="IVO76_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7428089.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            53949..54269
                     /locus_tag="IVO76_000094"
     CDS             53949..54269
                     /locus_tag="IVO76_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7428090.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT        15478 a        11624 c         9451 g        17734 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc agtttctcgg
    17221 tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt ttcttcatac
    17281 tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca tatcataata
    17341 cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt ctaataagtt
    17401 cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg ccacgatatt
    17461 actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag tccctgtttt
    17521 tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag gatcggtacc
    17581 atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag aaatattgac
    17641 cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc cattttcgtc
    17701 attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat tagcgactgt
    17761 tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa atgccaaatc
    17821 ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact tcttcatata
    17881 atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag tcgttgtacc
    17941 aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt cttcatctaa
    18001 actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg ccactttgat
    18061 catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa cttgtccgct
    18121 tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct catcaatcaa
    18181 aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac cagccttgac
    18241 tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg tgatttcttt
    18301 ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct gaggattcat
    18361 agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca atgtttgata
    18421 attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag ataacattaa
    18481 attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc cttcattatc
    18541 aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt gtttttccaa
    18601 ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt tctctgcttc
    18661 atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt tcagtgaact
    18721 tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa gttcagaagc
    18781 gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga tatagacagt
    18841 gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta attgctcctc
    18901 gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt gttcgctgtt
    18961 ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat gtttgggatc
    19021 agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg gaacatcgat
    19081 cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt gtgttcctct
    19141 cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg tggagtcgta
    19201 gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact tcactcgcgc
    19261 ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca acgcaacaaa
    19321 taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg accggcgaaa
    19381 cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag aagtatttaa
    19441 tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac attcatgctt
    19501 cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag aaattcttta
    19561 cctaatttca agataaaatc aactttttgt ttcacagcaa tcacttaaag ttacatatcc
    19621 aactattttt ttcattaaaa aataaactta tttttgaaaa ttatgttttc ttaagtcgtg
    19681 aaacactttg atggatcatt tatttgtgga aaactacgtt ttgttattct aaaaaactta
    19741 tataaaaaat agtaaggttt ttatttcgaa tccataaaat attcttggat tgcttctcgt
    19801 gtttttttca ctttttcgct aagcttttcg tttttagcca tatagaaata aaaaatgata
    19861 tcgatcgtag caaattgggc caaaagtgaa ttagttgccg cacttcgatt cgtgatttct
    19921 tttggtctag atgtttgtac gagtacatcg gctatcttgc taagcgggtt atttccaaat
    19981 tgcgtcaacg caatgatttc gatattcatg cttttcgcca gctcggctaa tgcgactaca
    20041 tctgcacttc tacctgagtt tgaaacaagc caaagcactt tctttttctc attgctgaca
    20101 agttgcggga gcaagacgtg gatatctttt tcaaaaatga ttggcttacc gacacgagac
    20161 catttttgca gaatatcttc tactactaaa gaagaggcgc caaccccata taaaaaaaca
    20221 gtctctgccg attccaaaca ctctacgact ttttggatcg tttcttcttc tagaatatct
    20281 actgtttctt ttatcgctac ttgtgcattg ttaccaagtt tgtttttgat agagtaaaag
    20341 gactcatttg cttctatatc actaaaacct tcatcgattc cagtctgtag ttctgttgaa
    20401 atcgtgattt tgaagtccgt cagactttga aaaccgattt ttttggaaaa acgtacaact
    20461 gtcggcgcac tcaacccgga agcttctgct aattcattag ctgtcattgt agggaccttc
    20521 tcaatattac cgataatgta gttggccaac tttttttctg ctgaagaata atcttccatc
    20581 atactttcaa tacgactaat tacgctcata agttcctcca cccttttaat gatagtctat
    20641 gtaagtatac catatcagac ccttattcac ggtcgtatac tggataaaaa acaaaaaaac
    20701 aatccgccca acagaatatc ttatcccatc aatcccacag attctattga gcatctgtgt
    20761 ctttgaggaa tacctgttat ctattgttcg gattgctttc tatctactac ttttttacag
    20821 gaaatttacc tattagtttt ctctgctgta atttttgact tctgcttcca tagcatcaat
    20881 aaagatattc aaatcttcta cagctgtttc tttacttcca taacgacgta cgttcactgt
    20941 tgcatcttcc aattccttgt ctccaacaac taattggtaa gggatctttt gtgtttgaga
    21001 ggcacgtatc ttatagccca ttttttcatt acgatcatca acttctacac gcaacccctt
    21061 catttgcaaa cgttctttga tttcataagc ataatcgcca tgtgcatcaa cagaaacagg
    21121 gatgattgtt gcttgtatag gtgccaacca tgttggaaaa gcgcctttgt atacttctgt
    21181 taaataagcg acaaaacgtt ccattgtaga aacaattccg cggtggataa ctactggacg
    21241 atgcgtattt tccccgtctt ctccaacata tgtcaaatca aagcgctctg gcaataagaa
    21301 gtctaattgt atagttgata atgtttcttc tgtaccaaga gctgttttga cttgaacatc
    21361 tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca aagtaatcta gttctaattc
    21421 atccatagcg gctttcagca ttgcttgggc tttttcccac attgcgtcat catcaaaata
    21481 tttatctgta ttgtttggat cgcgatagct caagcggaaa cgataatctg taatattgaa
    21541 gtcagcatat actgccacca tcaattcaag cgtacgttta aattcatctt tgatttgatc
    21601 aggacgaaca aatgtatgac catcattcaa cgtcatttct cgtacacgtt gtaaacctga
    21661 caaagcacct gatttttcat agcgatgcat cattcctaat tcagcgatac gaattggtaa
    21721 ttcacgataa gaatggatcg tgtttttgta gaccatcatg tgatgagggc agttcattgg
    21781 gcgaagaaca agcatttcgc catctcccat atccattggc gggaacatgt cttcttgata
    21841 atgatcccag tgtccagatg ttttgtacaa ctcaacatct cccattattg gtgtatatac
    21901 atgttggtaa cctagactaa cttctttgtc tacgatataa cgttcgatcg tgcgtcggat
    21961 tgttgcccct tttggaagcc agaatggcaa accagaacct acttctgggg aaaccatgaa
    22021 caagtcaagt tcttttccta gtttacgatg gtctctttct tttgcttctt cacggagacg
    22081 gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc ccgtatacac gttgcatcat
    22141 ttgattgttt gagtttccac gccaataagc acctgccact gataataatt taaatacttg
    22201 tatacggcca gtggatggaa catgtggtcc acgacacaaa tcgatgaatt cgccttgttg
    22261 ataagcagta atcacttctt ctggcaattc actaatcagt tctactttgt acggatcact
    22321 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
    22381 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
    22441 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
    22501 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
    22561 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
    22621 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
    22681 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
    22741 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
    22801 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
    22861 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
    22921 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
    22981 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
    23041 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
    23101 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
    23161 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
    23221 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
    23281 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
    23341 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
    23401 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
    23461 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
    23521 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
    23581 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
    23641 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
    23701 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
    23761 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
    23821 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
    23881 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
    23941 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
    24001 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
    24061 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
    24121 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
    24181 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
    24241 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
    24301 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
    24361 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
    24421 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
    24481 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
    24541 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
    24601 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
    24661 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
    24721 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
    24781 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
    24841 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
    24901 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
    24961 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
    25021 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
    25081 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
    25141 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
    25201 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
    25261 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
    25321 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
    25381 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
    25441 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
    25501 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
    25561 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
    25621 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
    25681 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
    25741 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
    25801 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
    25861 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
    25921 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
    25981 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
    26041 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
    26101 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
    26161 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
    26221 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
    26281 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
    26341 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
    26401 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
    26461 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
    26521 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
    26581 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
    26641 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
    26701 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
    26761 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
    26821 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
    26881 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
    26941 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
    27001 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
    27061 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
    27121 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    27181 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    27241 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    27301 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    27361 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    27421 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    27481 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    27541 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    27601 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    27661 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    27721 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    27781 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    27841 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    27901 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    27961 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    28021 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    28081 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    28141 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    28201 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    28261 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    28321 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    28381 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    28441 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    28501 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    28561 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    28621 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    28681 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    28741 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    28801 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    28861 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    28921 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    28981 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    29041 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    29101 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    29161 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    29221 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    29281 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    29341 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    29401 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    29461 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    29521 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    29581 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    29641 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    29701 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    29761 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    29821 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    29881 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    29941 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    30001 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    30061 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    30121 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    30181 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    30241 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    30301 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    30361 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    30421 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    30481 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    30541 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    30601 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    30661 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    30721 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    30781 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    30841 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    30901 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    30961 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    31021 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    31081 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    31141 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    31201 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    31261 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    31321 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    31381 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    31441 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    31501 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    31561 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    31621 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    31681 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    31741 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    31801 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    31861 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    31921 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    31981 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    32041 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    32101 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    32161 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    32221 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    32281 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    32341 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    32401 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    32461 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    32521 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    32581 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    32641 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    32701 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    32761 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    32821 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    32881 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    32941 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    33001 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    33061 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    33121 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    33181 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    33241 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    33301 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    33361 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    33421 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    33481 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    33541 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    33601 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    33661 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    33721 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    33781 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    33841 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    33901 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    33961 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    34021 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    34081 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    34141 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    34201 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    34261 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    34321 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    34381 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    34441 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttgga tctcattata
    34501 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    34561 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    34621 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    34681 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    34741 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    34801 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    34861 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    34921 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    34981 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    35041 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    35101 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    35161 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    35221 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    35281 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    35341 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    35401 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    35461 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    35521 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    35581 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    35641 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    35701 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    35761 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    35821 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    35881 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    35941 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    36001 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    36061 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    36121 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    36181 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    36241 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    36301 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    36361 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    36421 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    36481 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    36541 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    36601 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    36661 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    36721 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    36781 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    36841 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    36901 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    36961 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    37021 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    37081 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    37141 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    37201 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    37261 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    37321 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    37381 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    37441 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    37501 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    37561 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    37621 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    37681 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    37741 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    37801 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    37861 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    37921 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    37981 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    38041 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    38101 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    38161 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    38221 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    38281 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    38341 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    38401 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    38461 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    38521 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    38581 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    38641 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    38701 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    38761 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    38821 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    38881 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    38941 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    39001 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    39061 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    39121 ttgttcagat atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    39181 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    39241 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    39301 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    39361 aatgaattac ttattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    39421 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    39481 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    39541 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    39601 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    39661 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    39721 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    39781 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    39841 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    39901 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    39961 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    40021 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    40081 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    40141 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    40201 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    40261 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    40321 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    40381 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    40441 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    40501 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    40561 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    40621 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    40681 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    40741 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    40801 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    40861 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    40921 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    40981 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    41041 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    41101 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    41161 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    41221 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    41281 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    41341 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    41401 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    41461 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    41521 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    41581 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    41641 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    41701 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    41761 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    41821 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggta gcttctgtca
    41881 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    41941 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    42001 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    42061 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    42121 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    42181 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    42241 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    42301 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    42361 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    42421 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    42481 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    42541 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    42601 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    42661 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    42721 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    42781 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    42841 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    42901 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    42961 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    43021 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    43081 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    43141 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    43201 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    43261 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    43321 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    43381 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    43441 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    43501 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    43561 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    43621 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    43681 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    43741 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    43801 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    43861 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    43921 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    43981 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    44041 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    44101 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    44161 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    44221 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    44281 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    44341 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    44401 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    44461 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    44521 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    44581 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    44641 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    44701 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    44761 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    44821 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    44881 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    44941 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    45001 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    45061 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    45121 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    45181 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    45241 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    45301 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    45361 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    45421 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    45481 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    45541 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    45601 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    45661 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    45721 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    45781 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    45841 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    45901 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    45961 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    46021 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    46081 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    46141 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    46201 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    46261 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    46321 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    46381 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    46441 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    46501 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    46561 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    46621 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    46681 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    46741 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    46801 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    46861 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    46921 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    46981 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    47041 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    47101 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    47161 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    47221 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    47281 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    47341 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    47401 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    47461 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    47521 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    47581 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    47641 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    47701 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    47761 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    47821 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    47881 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    47941 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    48001 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    48061 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    48121 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    48181 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    48241 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    48301 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    48361 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    48421 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    48481 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    48541 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    48601 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    48661 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    48721 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    48781 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    48841 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    48901 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    48961 acatagccta caaactgtca ctcaaactta agccctattt ttgtctattc ttactgacaa
    49021 aaacgttttc ttaaaaaaga acaaaaaaac tggcgtagct cttttacaag ccacaccagc
    49081 ttttgatacg ttgtttattt tacatattca tgaacaagtt caacgacttc ttccatcata
    49141 tcgcattctt tcaatgcacg atcagcaagt tcagccattt tcgctgtgtc caagcgtttc
    49201 attaaactac gcgttttaag aatagatgtc gcactcattg agaactcatc tagacccata
    49261 cctactagca atggaacagc tgtttgatct ccagccattt ctccacacat acctgcccat
    49321 tttccttcag cgtgtgctgc atcaattaca tttttgatca aacgtaaaat tgatgggtta
    49381 tatggttggt acaagtaaga aacgcgttcg ttcatacggt ctgctgccat tgtgtattga
    49441 atcaaatcgt tcgtacctac agagaagaaa tctacttctt ttgcaaactt gtctgctaag
    49501 actgctgctg ctggaatctc gatcatgatg ccgacttgga tcgtatcaga tacttccttg
    49561 ccttcactta tcaatttttg tttttcttct tcaaagatcg cttttgctgc gcggaattct
    49621 ttgagtgtag ctaccatagg gaacatgata cgtaaattac catgaacaga tgcacgaagc
    49681 aatgcacgca tttgtgtacg gaacatttcg tcgccttgtt cagataaact gatacgcaat
    49741 gcacgatatc ctaagaatgg attcatttca tgaggtaatt gcagatatgg tagttcttta
    49801 tctccaccga tgtccattgt acgaacaact acaggttttc cttccatacc ttctaataca
    49861 gctttgtaag cttcatattg gtcatcttct gttgggaagt ctggagaatc catatacagg
    49921 aattcagtac ggtacagacc aactgcttct ccaccattat tatggacacc aactagatct
    49981 tttggtgttc cgatgtttgc agctaattca aaatgcttgc cgtcagctgt gacagtttct
    50041 gcatttttta atttttccca ttctgctttt tgtgctgcgt aatctgcacc tgctttttca
    50101 aattcagctt tttgttcatc tgtaggatcg atgattacat cgccttcgat cccgttgaca
    50161 gccaaaatat cgccttcttt gactttagca gtaatttctt ttgtcccaac gattgctgga
    50221 atttctaaag aacgtgccat gatagcagaa tgtgaagtac gtccaccaat gtctgttaca
    50281 aacgctttta caaaattgcg atctaattgt gctgtatcac ttggagtcaa gtcatgtgca
    50341 acaacgacca cttcttcgtt gatcattgat ggatttggta aagtaacgcc taataagtgc
    50401 gctaaaatac gtttagcaac gtcacgaata tctgcagcac gctcttgcat ataagcatta
    50461 tcttccattg cttcgaacat accgatatac atatccgtta cttcttttag cgctgattca
    50521 gcatttacgc tgttgtcttt aatattttgc ttgatttgac ctaccatttc tggatctgaa
    50581 agaaccatca aatgggcatc aaatacttgc gcttctgctt cacctaagct ttgcgctgct
    50641 ttttcacgaa tttgctgcaa ttcttcagta gatttagcta atgcagcatc caaacgagct
    50701 tcttcagccg cagtatcttc tactgttgcc ttactgaatg ttaaatccgg ttgaactagc
    50761 aggtaagctt ttgcaacggc tacaccgtca ctagcggcaa tcccttttag catttcaacc
    50821 attattcaga taagccttct ttcttcattg tttcaacgat tgctgccatt gcatcagctt
    50881 cgtcagcacc ttctgcagta atagtaacat cagaaccttg acctacacct aaagacataa
    50941 cgcccatgat tgatttcaag tttactgatt tacctttata ctctaagttt acatcagagt
    51001 taaatttgct tgctgtttgt actaatagtg tagctggacg agcgtggatc ccagtttctg
    51061 ctactacgtg aaattctttc ttttccatat taatcggtct cctttaagtg agtgttgttt
    51121 ttatgtgagg gttttctttt ttagtaattc agagaatcct aacaaatgcc accctttcat
    51181 ctgtaaagat taacattttt tttcgttaga tacaacagtt ttcattcaat ttcagataaa
    51241 agtattcaaa ttattccgga ttctttttca tccttttctt cacattttat aacaaacgtg
    51301 tttatttcct ttttcttgtc gctttctctt ctcctgacgt ttatcattct ttcctttgac
    51361 cctctttgtt ttcatatgaa acgaattact tgcttccctt tttattttta gtataataag
    51421 agtcaaaggt caggaatggt caatagagat ttcacctttt ctacctcata aatctcttaa
    51481 aaaactaggg gttattcttt tacctcatga aaaacgagat gtaaaatatg gatgtaccca
    51541 tttttgtgaa aggacgtgaa tgattcatgc tttgtcaaaa ctgcggcaag aatgaggcaa
    51601 cgattcactt gtatgccact gtcaatggtc agcggacaca actcgattac tgtcagagct
    51661 gttatcaaaa aatcaaaaat caacaaaatg gaggtctgac taatatggcc caaaatgatc
    51721 catttggatt tggcagtttg gacgatcttt tccgttccat gtctcgccaa atgcaacagc
    51781 aaggaaatta tgagcaaaca ccaccaacac aatttggcgg aaacaacaac ttcaatggtg
    51841 gtcagccacc tcaaggcaat acagaaggtt tattgggaga atacggaatc aatatcacag
    51901 aacaagcacg acaaggtgat atcgatcctg tgatcggacg cgatgacgaa atcaaacgag
    51961 tcattgaaat tttaaatcgc cgaaccaaga acaatcctgt cttgattgga gaaccaggtg
    52021 ttggtaaaac agcagttgtt gaaggtttag cacaaaaaat cgtagatggt gatgtaccgc
    52081 aaaaattaat ggacaaagaa gtcattcgtt tagatgtcgt atctttagtc caaggtactg
    52141 gtatacgagg acaatttgaa gaacgtatgc aaaaattgat tgaagagatc cgtcaagcag
    52201 aaaatgtgat cctattcata gatgaagttc acgaaatcgt cggtgctggt tctgctggtg
    52261 atggaaatat ggatgctggt aatattttaa aaccagcttt agctcgagga gaattgcaaa
    52321 tggttggtgc tactacctta aatgagtacc gcatcatcga aaaagacgct gctttagaac
    52381 gtcgtatgca acctgtacgg gtagatgaac caactgtcga agaaacgatc agtattttga
    52441 aaggtttgca aaaacgttat gaagactatc atcatgtaaa atatactgat gaggccatcg
    52501 aagcagccgc aactttatcc aatcgttacg tccaagatcg cttcttgccg gataaagcca
    52561 tcgatttgct agatgaaaca ggttctaaga aaaacttgac gatccaaatc gttgatccta
    52621 aaacaatcga gaaaaaactg caagaagcag aagaacaaaa agttttagct tctcgagaag
    52681 aagatttcga aaaagcagct tattatcgcg atcagatcaa taaacttcaa aaaatgaaag
    52741 aacgtcagct cactgaagag gaaacacctg tgattaccga aaaagatatg gaaaaaatcg
    52801 ttgaacaacg gacaggtatt ccagtgggtg aattaaaaga aaaagaacag acacaactga
    52861 aaaacttagc agatgacttg aaagctcatg tcatcggaca agataacgct gtagatcgag
    52921 tagcgaaagc gattcgccga aaccgtgttg gattaaacaa acagaatcgt ccaatcggct
    52981 ccttcttatt tgttggacca actggtgtag gtaaaacaga gcttgcgaaa caacttgcct
    53041 acgaactatt cggttcacaa gattcaatga ttcgtttcga tatgtctgaa tatatggaaa
    53101 aacatagtgt ctctaaattg attggttctc ctccaggtta tgtcggatac gaagaagctg
    53161 gtcaattaac agaaaaagta cggagaaatc cttacagtct tgtcttgtta gatgaagtag
    53221 aaaaagctca tccagatgtt ttgcatatgt tcttgcaaat cttagatgat ggtcgattga
    53281 cagatgcaca aggaagaacg gtcagcttta aagatacgat catcattatg acaagtaatg
    53341 ccggaactgg taaagtagaa gcaaacgttg gttttggtgc ggcacgcgaa ggtgttactc
    53401 gttctgtatt gaaccaatta aacaactact ttacaccaga atttctgaac cgttttgacg
    53461 ggatcatcga attcagtgca ttatcaaaag aaaacttgat gacaatcgtc acactaatgc
    53521 tagacgatgt caaccaaatg ctggcagcac agcaacttca catcgaagtt cctacgaatg
    53581 tcaaagaaaa attagttgac ttaggctatg atccatctat gggtgctcgt cctcttcgac
    53641 gcacgatcca agaacaaatc gaagatggta tcgctgaatt ttatcttgat cacccttcta
    53701 tccatgagtt gaaagcaaaa ctggataaag atggaaaaat catcgttact tctaaaccag
    53761 aacgtttggc taaagaatca gccgaagaaa cggctgaata aatccagttt tatagcgtga
    53821 gacggtattt ctttgagaaa tgccgtctca ttcttttttc agaaaagtca aaatactgtt
    53881 cattttataa aaaagaaatt ataataagag aaacaagcaa ttaaaaatac ataaaagagg
    53941 tgagcgccat gaaattggta aatgtaacaa acagccattc ccgtcttgta ttaaaccagc
    54001 ttgaaaacac tgatgcccat ctagtcaagg tctacacagc cggaaatacc actattgtct
    54061 acactgaagc ccctgaacat aatgaaattc tgctgatcaa tgacaaacgt aaaatccagc
    54121 cgaaagaaat cgaagatgct aaaaattatt tcttagataa aatcaaagac gccgtttacc
    54181 acgaggatga aatcaaagta atcgaattgg caggccttgt tgaaatctcg attccaaaac
    54241 atgaattagg cgctacatta gccgtgtaaa caaattttga acataat
//
LOCUS       DACCQI010000003        54191 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCQI010000003 DACCQI010000000
VERSION     DACCQI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.003"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(16..702)
                     /gene="gpmA"
                     /locus_tag="IVO76_000095"
     CDS             complement(16..702)
                     /gene="gpmA"
                     /locus_tag="IVO76_000095"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7428091.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(874..1455)
                     /locus_tag="IVO76_000096"
     CDS             complement(874..1455)
                     /locus_tag="IVO76_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7428092.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1588..2271)
                     /gene="rpiA"
                     /locus_tag="IVO76_000097"
     CDS             complement(1588..2271)
                     /gene="rpiA"
                     /locus_tag="IVO76_000097"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7428093.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2590..3003
                     /gene="rpsL"
                     /locus_tag="IVO76_000098"
     CDS             2590..3003
                     /gene="rpsL"
                     /locus_tag="IVO76_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7428094.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3087..3557
                     /gene="rpsG"
                     /locus_tag="IVO76_000099"
     CDS             3087..3557
                     /gene="rpsG"
                     /locus_tag="IVO76_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7428095.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3651..5738
                     /gene="fusA"
                     /locus_tag="IVO76_000100"
     CDS             3651..5738
                     /gene="fusA"
                     /locus_tag="IVO76_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7428096.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5910..7097
                     /gene="tuf"
                     /locus_tag="IVO76_000101"
     CDS             5910..7097
                     /gene="tuf"
                     /locus_tag="IVO76_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7428097.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7504..7812
                     /gene="rpsJ"
                     /locus_tag="IVO76_000102"
     CDS             7504..7812
                     /gene="rpsJ"
                     /locus_tag="IVO76_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7428098.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7843..8472
                     /gene="rplC"
                     /locus_tag="IVO76_000103"
     CDS             7843..8472
                     /gene="rplC"
                     /locus_tag="IVO76_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7428099.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8500..9123
                     /gene="rplD"
                     /locus_tag="IVO76_000104"
     CDS             8500..9123
                     /gene="rplD"
                     /locus_tag="IVO76_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7428100.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9123..9413
                     /gene="rplW"
                     /locus_tag="IVO76_000105"
     CDS             9123..9413
                     /gene="rplW"
                     /locus_tag="IVO76_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7428101.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9452..10285
                     /gene="rplB"
                     /locus_tag="IVO76_000106"
     CDS             9452..10285
                     /gene="rplB"
                     /locus_tag="IVO76_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7428102.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10328..10606
                     /gene="rpsS"
                     /locus_tag="IVO76_000107"
     CDS             10328..10606
                     /gene="rpsS"
                     /locus_tag="IVO76_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7428103.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10629..10976
                     /gene="rplV"
                     /locus_tag="IVO76_000108"
     CDS             10629..10976
                     /gene="rplV"
                     /locus_tag="IVO76_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7428104.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            10990..11646
                     /gene="rpsC"
                     /locus_tag="IVO76_000109"
     CDS             10990..11646
                     /gene="rpsC"
                     /locus_tag="IVO76_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7428105.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11649..12083
                     /gene="rplP"
                     /locus_tag="IVO76_000110"
     CDS             11649..12083
                     /gene="rplP"
                     /locus_tag="IVO76_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7428106.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12073..12261
                     /gene="rpmC"
                     /locus_tag="IVO76_000111"
     CDS             12073..12261
                     /gene="rpmC"
                     /locus_tag="IVO76_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7428107.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12286..12552
                     /gene="rpsQ"
                     /locus_tag="IVO76_000112"
     CDS             12286..12552
                     /gene="rpsQ"
                     /locus_tag="IVO76_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7428108.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12610..12978
                     /gene="rplN"
                     /locus_tag="IVO76_000113"
     CDS             12610..12978
                     /gene="rplN"
                     /locus_tag="IVO76_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7428109.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13015..13323
                     /gene="rplX"
                     /locus_tag="IVO76_000114"
     CDS             13015..13323
                     /gene="rplX"
                     /locus_tag="IVO76_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7428110.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13351..13890
                     /gene="rplE"
                     /locus_tag="IVO76_000115"
     CDS             13351..13890
                     /gene="rplE"
                     /locus_tag="IVO76_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7428111.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13909..14094
                     /locus_tag="IVO76_000116"
     CDS             13909..14094
                     /locus_tag="IVO76_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7428112.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14130..14528
                     /gene="rpsH"
                     /locus_tag="IVO76_000117"
     CDS             14130..14528
                     /gene="rpsH"
                     /locus_tag="IVO76_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7428113.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14560..15096
                     /gene="rplF"
                     /locus_tag="IVO76_000118"
     CDS             14560..15096
                     /gene="rplF"
                     /locus_tag="IVO76_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7428114.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15257..15613
                     /gene="rplR"
                     /locus_tag="IVO76_000119"
     CDS             15257..15613
                     /gene="rplR"
                     /locus_tag="IVO76_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7428115.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15634..16134
                     /gene="rpsE"
                     /locus_tag="IVO76_000120"
     CDS             15634..16134
                     /gene="rpsE"
                     /locus_tag="IVO76_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7428116.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16149..16328
                     /gene="rpmD"
                     /locus_tag="IVO76_000121"
     CDS             16149..16328
                     /gene="rpmD"
                     /locus_tag="IVO76_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7428117.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16372..16812
                     /gene="rplO"
                     /locus_tag="IVO76_000122"
     CDS             16372..16812
                     /gene="rplO"
                     /locus_tag="IVO76_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7428118.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16812..18107
                     /gene="secY"
                     /locus_tag="IVO76_000123"
     CDS             16812..18107
                     /gene="secY"
                     /locus_tag="IVO76_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7428119.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18169..18816
                     /locus_tag="IVO76_000124"
     CDS             18169..18816
                     /locus_tag="IVO76_000124"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7428120.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19007..19225
                     /gene="infA"
                     /locus_tag="IVO76_000125"
     CDS             19007..19225
                     /gene="infA"
                     /locus_tag="IVO76_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7428121.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19258..19374
                     /gene="rpmJ"
                     /locus_tag="IVO76_000126"
     CDS             19258..19374
                     /gene="rpmJ"
                     /locus_tag="IVO76_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7428122.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19392..19757
                     /gene="rpsM"
                     /locus_tag="IVO76_000127"
     CDS             19392..19757
                     /gene="rpsM"
                     /locus_tag="IVO76_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7428123.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19785..20174
                     /gene="rpsK"
                     /locus_tag="IVO76_000128"
     CDS             19785..20174
                     /gene="rpsK"
                     /locus_tag="IVO76_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7428124.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20254..21192
                     /locus_tag="IVO76_000129"
     CDS             20254..21192
                     /locus_tag="IVO76_000129"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7428125.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21220..21600
                     /gene="rplQ"
                     /locus_tag="IVO76_000130"
     CDS             21220..21600
                     /gene="rplQ"
                     /locus_tag="IVO76_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7428126.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21872..22711
                     /locus_tag="IVO76_000131"
     CDS             21872..22711
                     /locus_tag="IVO76_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7428127.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22687..23556
                     /locus_tag="IVO76_000132"
     CDS             22687..23556
                     /locus_tag="IVO76_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7428128.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23546..24343
                     /locus_tag="IVO76_000133"
     CDS             23546..24343
                     /locus_tag="IVO76_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7428129.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24546..25292
                     /gene="truA"
                     /locus_tag="IVO76_000134"
     CDS             24546..25292
                     /gene="truA"
                     /locus_tag="IVO76_000134"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7428130.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25625..28294)
                     /locus_tag="IVO76_000135"
     CDS             complement(25625..28294)
                     /locus_tag="IVO76_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7428131.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28532..28720)
                     /locus_tag="IVO76_000136"
     CDS             complement(28532..28720)
                     /locus_tag="IVO76_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7428132.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            28990..29352
                     /locus_tag="IVO76_000137"
     CDS             28990..29352
                     /locus_tag="IVO76_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7428133.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29404..31086
                     /locus_tag="IVO76_000138"
     CDS             29404..31086
                     /locus_tag="IVO76_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7428134.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31203..33239
                     /gene="recG"
                     /locus_tag="IVO76_000139"
     CDS             31203..33239
                     /gene="recG"
                     /locus_tag="IVO76_000139"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7428135.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33288..34289
                     /gene="plsX"
                     /locus_tag="IVO76_000140"
     CDS             33288..34289
                     /gene="plsX"
                     /locus_tag="IVO76_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7428136.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34411..34653
                     /gene="acpP"
                     /locus_tag="IVO76_000141"
     CDS             34411..34653
                     /gene="acpP"
                     /locus_tag="IVO76_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7428137.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34921..35925
                     /locus_tag="IVO76_000142"
     CDS             34921..35925
                     /locus_tag="IVO76_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428138.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35926..36870
                     /locus_tag="IVO76_000143"
     CDS             35926..36870
                     /locus_tag="IVO76_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428139.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36870..37832
                     /locus_tag="IVO76_000144"
     CDS             36870..37832
                     /locus_tag="IVO76_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428140.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37859..38773
                     /locus_tag="IVO76_000145"
     CDS             37859..38773
                     /locus_tag="IVO76_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428141.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38799..40580
                     /locus_tag="IVO76_000146"
     CDS             38799..40580
                     /locus_tag="IVO76_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7428142.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40928..41614
                     /locus_tag="IVO76_000147"
     CDS             40928..41614
                     /locus_tag="IVO76_000147"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7428143.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41634..45215
                     /gene="smc"
                     /locus_tag="IVO76_000148"
     CDS             41634..45215
                     /gene="smc"
                     /locus_tag="IVO76_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7428144.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45224..46033
                     /locus_tag="IVO76_000149"
     CDS             45224..46033
                     /locus_tag="IVO76_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7428145.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46044..47042
                     /gene="ftsY"
                     /locus_tag="IVO76_000150"
     CDS             46044..47042
                     /gene="ftsY"
                     /locus_tag="IVO76_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7428146.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47229..49496
                     /gene="gshAB"
                     /locus_tag="IVO76_000151"
     CDS             47229..49496
                     /gene="gshAB"
                     /locus_tag="IVO76_000151"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7428147.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49585..50037)
                     /locus_tag="IVO76_000152"
     CDS             complement(49585..50037)
                     /locus_tag="IVO76_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7428148.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50187..51137
                     /locus_tag="IVO76_000153"
     CDS             50187..51137
                     /locus_tag="IVO76_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7428149.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51130..52089
                     /locus_tag="IVO76_000154"
     CDS             51130..52089
                     /locus_tag="IVO76_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7428150.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52086..52841
                     /locus_tag="IVO76_000155"
     CDS             52086..52841
                     /locus_tag="IVO76_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7428151.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52864..53817
                     /locus_tag="IVO76_000156"
     CDS             52864..53817
                     /locus_tag="IVO76_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7428152.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
BASE COUNT        17781 a         9820 c        11669 g        14921 t
ORIGIN      
        1 tttttatagt gttttttata ggtagtattt tttagcaact gttaagtcat catttaattc
       61 ataaacaagt ggttgacctg ttggaatttc aagatccatg atgtcttcgt cagagatacc
      121 ttcgatatgt ttagctaggg cgcgtaatga gttaccatgt gcagctacta aaacagtttt
      181 gttgtctagt aatgctggag caatttcatc ttgccagaat ggcaaagcgc gttctaatgt
      241 cactttcaag ttttctccac caggaatatc gcgtttgtct aacattgcgt aacgacggtc
      301 atttgctgca gaaccttcgt cagtttcttc cattaatgga ggaagagtat cgtaagaacg
      361 acgccagatg tgcacttgtt cgtcgccgta tttttcagct gtttcttttt tgttcaaacc
      421 ttgtaattta ccatagtgac gttcgtttaa gcgccatgat ttgatttgtg gtacccaaag
      481 ttgatctgaa tattctaaga tcaagttaca tgttttgatt gcacgagtta aaacagaagt
      541 gtaagcaaca tcaaattcga ttccagcttc tttgattttg cgtccgcctt cttttgcttc
      601 ttcgattcct tctggtgcta gatttacatc agcccaacca gtaaattggt tcaatgcatt
      661 ccattcacta agaccgtgac gagaaaaaac taattttggc atgtttagct ctccttttaa
      721 gataggttta tatttctgaa attttcagtt ctgttttatt gtacaatttt ttggaagaga
      781 tttccatcga aaaattagat aagattctat gagaaaacaa gtagagaata taaaaaaacg
      841 aacagtgccc acgcaactgt tcgtttttag gtattagatg cataattgct cgatttcttt
      901 ttcaggtaaa ttgatatatt tataacatgt tccaactgaa acaccgcatt ttgaagaaat
      961 aaattggatc gtttcttttt tttcataata gagacgtcgg atttttttga tcgtttttgc
     1021 atcgattttt ggtcttccgc cgatttttcc ttttttacga gcttcatcta atccaactaa
     1081 tgtacgttct ttgatcaatg cgcactccat gtttgctagg ttttccatta attggaaata
     1141 gatttttccc atttcgcgcg tatcgattcc ttcatcaaga ctaatcaagt gcagcccgcg
     1201 atctttgaat aattgcgtga gttctgttaa ttgccgcgta gatttgccta atcgatgaag
     1261 ttggcaaatg actagtgcat cgccttgttg catgtcagcg atcactgact ccaaatgaac
     1321 agcagtagaa tcattttgtt ccacttcata tgtctcatgg aaaatttctt gacaaccaaa
     1381 gtcagataat tgatcaacct gagttgttaa atcaacgtca tttatggttg tgcgtgcata
     1441 accaataatt ttcatggtga ttcctttcta gcagtgattt tctaactgga tataacagta
     1501 aaaatgtagc ataaagtacg tcaaaaagat agttttttcg caaattttcc gctgtctttt
     1561 tttattggat gatcaagcag tttttcttta tttacgacgg attgcgtcaa gaatctcaac
     1621 gcctttttct gagccgacaa ctactttaga aaccatgttt aggaaaagcc catgttctac
     1681 taccccaaca agttcatcta aataggtagc taagctttct ggctgttcga tgacttctag
     1741 atgcagatca atgatgtaat gaccaccatc tgtggttaag acttctccgt tttcgtctgt
     1801 acgcaagacg ggttggaatc ttttttcttc aaaaatatga agtagctgtt gggagccata
     1861 agggatcact tcaactggga gtgggaactt cccaagttta tcgactaatt tagaagaatc
     1921 aacgatccaa atcgtttctt ttgagtacgt agcgactatt ttctcgaaaa gcaaagcagc
     1981 gccgccgcct ttgatccctt gaaaatcagc actgatttca tcagcaccgt ctattgtcaa
     2041 atcgactacg gggacttcat caatactctt taacggtatt cctaattttt ccgcttgttc
     2101 ttttgtcgca tttgaagtag taactcctgt gatacgtaaa ccttcttcac gcatacggcg
     2161 accaatctct tctaccatat aataggcggt tgaaccggtc ccgagaccta cgatcatccc
     2221 gtcctttacg aatttcgcgg cttcaatgcc agctaattgt tttaaattca tctttgcgtc
     2281 cacctttttt tagtttgagc atcgtgcttt cctcactatt ttagcgtgat ttacggtaaa
     2341 aagataccat tcttttttat tccaataaag ctttttcaaa aaaaattgca tctcttgttg
     2401 acatgttttt tcattcgtga tattctaaca aaggtgcttt atctacaggt ttctgtgaag
     2461 aaacgtgctg actgtttatc aaagcatatt tgataaggat gcagacattg catttatttt
     2521 ttatcacaaa aaaagaacca cctggatgtg tggaactaga cgcaaatcgt gggaaggagg
     2581 aaacaaggaa tgcctacaat taaccaatta gtacgtaaac ctcgtaaatc aaaggtggaa
     2641 aaatctaatt cacctgcttt gaacaaagga tataacagct ttaagaaaac tcaaactaac
     2701 gtaaactcac ctcaaaaacg tggggtagca actcgtgtgg gtacaatgac acctaaaaaa
     2761 ccgaactcag ctttacgtaa atatgcacgt gttcgtttgt caaacttaat tgaagttact
     2821 gcttatatcc cagggattgg tcataactta caagaacaca gtgttgtatt attacgcggt
     2881 ggtcgtgtaa aagacttacc aggggtacgt taccatatcg ttcgcggtgc tttagatact
     2941 gctggtgtta acgaccgtaa acaaagccgt tctaaatacg gaactaaaaa acctaaagct
     3001 taattttaat tattaaacaa atgaaacttg cttctactat atagaagtaa ttactatgaa
     3061 caatatttcg gaaggaggag ttacggatgc cacgtaaagg tcctgttgca aaacgtgatg
     3121 ttttaccaga tcctatttat aactcaaaat tagtaactcg cttgatcaac cgtgtaatgg
     3181 ttgacggaaa acgcgggatt gcagctaaca ttatctataa tgcctttgac ttgatcaaag
     3241 aatctacagg taacgatcca ttagaagtgt ttgaacaagc aatgaaaaac gttcaacctg
     3301 ttcttgaagt taaagcacgt cgtgttgggg gttcaaacta ccaagtacca gttgaagttc
     3361 gccctgaacg ccgcgttact ctaggtcttc gttgggtagt aaactacgct cgcttgcgcg
     3421 gtgaacacac aatggaagaa cgcttagcaa aagaaatcat ggatgctgca aacaacacag
     3481 gtgcttctgt taaaaaacgt gaagacactc ataaaatggc tgatgccaac cgtgcgtttg
     3541 ctcactatcg ctggtaagat tctctctgtt tggtcggctt ttataagtcg acctcagata
     3601 gtgtccttat ttttagcaac tagatagtaa agagaggaga aacaaggaga atgaccagag
     3661 aattttcttt acaaaacact cgtaatatcg gtatcatggc ccacatcgat gctggtaaaa
     3721 cgactgctac tgaacgtatc ttgtactata ctggtaaaat ccataaaatt ggtgaaactc
     3781 atgaaggagc ttcacaaatg gactggatgg aacaagaaca agaacgtggt attacgatca
     3841 cttctgctgc tactacagca caatggaagg gtcaccgaat caacatcatc gacacaccag
     3901 gacacgtaga cttcacagtt gaagttgaac gttcattgcg tgtacttgat ggtgctgtaa
     3961 ctgttcttga tgcacaatca ggtgttgaac ctcaaactga aacagtttgg cgtcaagcaa
     4021 caacttatgg tgtacctcgt atcgtatttg caaacaaaat ggacaagatc ggtgctgact
     4081 tcttatattc agtatcaact ttacatgacc gtttgcaagc aaacgctcac ccaatccaat
     4141 taccaattgg tgcggaagac gacttcacag gaattatcga ccttgtaaca atgaaggctg
     4201 aaatgtatac aaacgatctt ggaacagaga tcgaagaaac agaaattcct gaagaatacc
     4261 gcgaattagc tgaagaatgg cgcgaaaaat tggttgaagc tgtagctgaa actgatgaag
     4321 aacttacttt gaaatactta gaaggtgaag aaatcactga agctgagtta aaagaaggta
     4381 tccgccgcgc aacagttaac gtagaattct acccagtact atgtggttca gcatttaaga
     4441 acaaaggggt tcaattacta ttggacgctg ttcttgatta cttgccatca ccgttagata
     4501 tcccagctat caaagggatt gaccctaaaa ctgatgaaga agtagaacgt cctgcagacg
     4561 acagcgctcc tttctcagct ttagcattta aagttatgac tgacccattc gtaggtcgtt
     4621 taactttctt ccgtgtgtac tctggtgttt tacaatcagg ttcatacgta caaaacgcaa
     4681 caaaaggcaa acgtgaacgt gttggtcgta tcctacagat gcatgccaac tctcgttcag
     4741 aaatctctga agtttacgct ggagatatcg ctgctgctgt tggtttgaaa gacacaacta
     4801 ctggggacac actatgtgat gaaaagagtc tagttattct tgaatccatg gaattcccag
     4861 aaccagttat ccaagtcgct attgaaccta aatcaaaagc tgaccaagat aaaatgggtg
     4921 tggctttaca aaaactatct gaagaagatc ctactttccg tgctgaaaca aacgtggaaa
     4981 caggcgaaac aatcatcgct ggtatgggtg aacttcactt agatatcatc gttgaccgta
     5041 tgcgtcgtga attcaaagtt gaagctaacg taggtgcgcc tcaagtatct taccgtgaaa
     5101 ctttccgtgc tggcactcaa gccgaaggta agtttgtacg tcagtctggt ggtaaaggtc
     5161 aatacggtca cgtatggatt gaatttacac caaatgaaga aggtgctggt ttcgaatttg
     5221 aaaatgcgat cgtcggtggg gttgttcctc gtgaatacat tccagctgtt gaaactggtt
     5281 tgaaagatgc tatggaaaat ggtgtcttag ctggctatcc attagtagat atcaaagcta
     5341 aactttacga tggttcatac catgatgtcg attcaaatga aactgccttc cgtgttgctg
     5401 cttctatggc attacgtgca gcagctaaga aagctaatcc agtaatcctt gaaccaatca
     5461 tggctgtaga agttgttatc cctgaagatt atttaggtga tgttatgggt cacgtaactg
     5521 ctcgtcgtgg acgtgttgaa ggtatggaag ctcgcgctgg cggccaacaa gttgttcgtg
     5581 cgatggttcc acttgctgaa atgtttggtt atgctacaac tcttcgttct gctactcaag
     5641 gtcgtggtac atttacaatg acatttgacc actacgaaga tgtaccaaaa tcagtacaag
     5701 aagaaatcat taagaaaaac ggcggaaaag ctgaataatt tcttaaaacc ggatagtcct
     5761 gcaaagagat tatccaaaga gactatattt ttaatatagt ttcgtgtaaa ataattatcg
     5821 atgatatggg catgtggtgt accttgtgta catcatatac ctatcagaca aataaaaaat
     5881 atattgtatt tttaggagga cattttaaaa tggcaaaaga aaaatttgac cgttctaaac
     5941 cacacgttaa cattggtact atcggacacg ttgaccatgg taaaactaca ttaacagctg
     6001 caatcacaac tgtactatct aagaaaaacg gcggccaagc tatggcttac gatcaaatcg
     6061 atggtgctcc agaagaacgc gaacgcggaa tcacaatctc aacagctcac gttgagtatg
     6121 aaacagatac acgccactac gctcacgttg actgcccagg acacgcggat tacgttaaaa
     6181 acatgatcac tggtgctgct caaatggacg gagctatctt ggtagtttct gctgctgacg
     6241 gcccaatgcc tcaaactcgt gaacacatcc tattgtctcg tcaagttggt gttccttaca
     6301 tcgttgtatt cttgaacaaa gtagacatgg ttgatgacga agaattacta gaattagttg
     6361 aaatggaagt tcgtgaccta ttaacagaat acgaattccc tggtgacgat gttcctgtag
     6421 ttgctggatc agctttgaaa gctctagaag gcgacgcttc atacgaagaa aaaattcttg
     6481 aattaatggc tgcagttgac gaatacatcc caactccaga acgtgacaac gacaaaccat
     6541 tcatgatgcc agttgaagac gtgttctcaa ttactggacg tggtactgtt gctacaggtc
     6601 gtgttgaacg tggacaagtt cgcgttggtg acgaagttga agttgttggt attgctgaag
     6661 aaacttcaaa aacaacagtt actggtgttg aaatgttccg taaattgtta gactacgctg
     6721 aagctggaga caacattggt gctttactac gtggtgttgc acgtgaagac atccaacgtg
     6781 gacaagtttt agctaaacca ggtacaatca cacctcatac aaaattctct gcagaagtat
     6841 acgtgttgac aaaagaagaa ggtggacgtc atactccatt cttcactaac taccgtccac
     6901 aattctactt ccgtacaact gacgtaacag gtgttgttga attaccagaa ggaactgaaa
     6961 tggttatgcc tggcgacaac gtaacaatgg aagttgaatt aatccaccca atcgctatcg
     7021 aaaacggtac taaattctca atccgtgaag gcggacgtac agttggtgcc ggtgtcgtta
     7081 cagaaatcaa agcttaattc acttaagttt taaagtaaag caacatttat tcggacgtaa
     7141 tcgctcggaa cgaaggtctt aggatctttg ttccgagctt ttttatttat tgcaggatca
     7201 ggaaaaaaga caggagtatt ataaatagaa gaaacacgaa ataaaagagt cagtattttc
     7261 taaatgaaac tttatgaaaa caaaatgttt tttacaagaa aaagccttgc tttgtgagtt
     7321 tatatatatt ataatgacaa aggtgcgttt actttagtga attgcacaga cgtaatcaga
     7381 atgatttcgt cggctaagaa acgagaggtt gcgacacgcc cggacgcttt gccacgaacg
     7441 agcgtgacgg aaattttcgt ggagctatgt ctacttttca aaataggcga aggagggaac
     7501 aagatggcaa aacaaaaaat tcgtatccgt ttaaaagcgt atgaacaccg tattttagat
     7561 caatcagcgg ataaaatcgt ggaaactgca aaaagaactg gagctagcgt gtctggtcca
     7621 attccattac caactgaacg cagtctttac acaatcatcc gtgcgactca taaatacaaa
     7681 gattcacgcg aacaattcga aatgcgtaca cacaaacgtc ttatcgacat tgtgaaccca
     7741 acaccaaaaa ctgttgatgc tttgatgaag ctagacttac catctggtgt aaacatcgaa
     7801 atcaaattat aaaactaaaa caaaaataat ggaggtgtac tcatgaccaa aggaatctta
     7861 gggaaaaaag tgggaatgac acaaatcttc actgaatctg gtgaattaat tccagtaact
     7921 gttgtagaag ctactccaaa cgtagtacta caagtgaaaa caatggaaac ggatggctac
     7981 gaagctattc aagttggtta ccaagatatg cgtgaagttt tatcaaacaa acctgcgaaa
     8041 ggtcatgttg caaaagcaaa cacggctcct aagcgcttca ttcgtgaatt caaaaatgtt
     8101 gagctaggag aatatgaagt aggaaaagaa attacagtag atgttttcca agcaggagac
     8161 attattgatg taacaggtac tacgaaaggt aaaggattcc aaggggttat caaacgccac
     8221 ggacaaagcc gcggaccaat ggctcacggt tctcgttacc atcgtcgtcc tgggtcaatg
     8281 ggtccagttg cacctaaccg tgtatttaaa aacaaacgtc ttgctggccg tatgggtggc
     8341 aaccgcgtaa ctatccaaaa cttggaagtt gtacgtgtcg atgcagaaag aaatgttatc
     8401 ttgatcaaag gtaatgttcc tggagctaaa aaatctttaa ttactatcaa atcagctgtg
     8461 aaagctaaat aattgtagga agaaaggagg aactaaggaa tgccgaatgt agcattattc
     8521 aaacaagatg gaagccaaaa cggtgaaatc acacttaacg aagaaatctt cggaattgaa
     8581 cctaatgaaa gtgttgttta cgatgcaatc gtaatgcaac gtgcgtcatt aagacaagga
     8641 acacacgcgg ttaaaaaccg tagcgctgtt cgcggcggcg gacgtaaacc atggcgtcaa
     8701 aaaggaacag gtcgtgcacg tcaaggatct atccgctcac cacaatggcg tggaggtggc
     8761 gtagtctttg gaccaacacc acgttcttac agctacaaac ttcctaaaaa agttcgtcgt
     8821 ttagcaatga aatctgtatt gtcagaaaaa gttgctgaaa acaacttggt agcaatcgaa
     8881 ggcttgaact tcgacgcacc aaaaacaaaa gaattcaaac aagttcttgc taacttatct
     8941 atcgactcta aagtattggt tgttctagaa aaaggtaacg actttgctgc tttatcagca
     9001 cgtaacttac caaacgtttc tgtagtaact tctgataatg tgagtgtgct agacgttgta
     9061 tcaaatacaa aagtgttagc aacacaaact gctcttactc aaattgagga ggtgcttgca
     9121 taatgaactt actagacgta atcaaacgcc cagtgatcac tgaaaaatca atgcttgcca
     9181 tggatgacaa gaaatacact tttgaagtgg acactcgcgc aaacaaaact ttagtaaaac
     9241 aagcggtaga agctgctttt gacgttaaag tcaaaaacgt gaacatcgtg aacgtacgtg
     9301 cgaaattcaa acgtatgggc aaatatgcag gatatacaaa aaaacgtcgc aaagcgatcg
     9361 ttacattgac tgaagattca aaagaaatcc aattattcga agctgctgaa taagcactaa
     9421 tagataactc attaaattag gagggaaaaa cgtggcgatt aaaaagtaca aacctacctc
     9481 aaatggtcgt cgtaacatga caggttctga ttttgctgaa atcacaacat caacacctga
     9541 aaaatcattg ttacaaccat tgaaaaacca tgctggacgt aacaacaacg gccgcatcac
     9601 tgtacgtcat caaggtggcg gacacaaacg ccaatatcgc gtgatcgact tcaaacgtaa
     9661 caaagataac gttgttgcaa ctgttcaaac gatcgaatat gatccaaacc gttcagctaa
     9721 catcgcgtta gtacactatg aagatggagt gaaagcttac atcttagcac ctaaaggact
     9781 agaagtgggt atgaaacttg tttcaggtcc agaagcagat atcaaagtcg gaaatgctct
     9841 tccattagaa aacattccag ttggtactgt tatccacaac attgaaatga aacctggtaa
     9901 aggtggacaa ttgatccgtt ctgctggtac aagtgctcaa gtacttggta aagaaggcaa
     9961 atacgtattg atccgcttga actctggtga agttcgtatg atcttagctg catgccgtgc
    10021 tacaatcggt tctgtaggta acgaacaaca cgaattaatc aacatcggta aagctggacg
    10081 ctctcgttgg atgcgtaaac gcccaactgt acgtggtagc gtaatgaacc ctaatgatca
    10141 cccacacggt ggtggtgaag gtaaagctcc tatcggacgt aaagcaccag tttctccttg
    10201 gggtcaacca gctcttggtt acaaaactcg taacaaaaaa gctaaatcag acaaacttat
    10261 tgttcgtcgt cgcaaaaata aataattact ttgaccatgt gtcgcacatt ttgagaggag
    10321 gttcaccatg ggtcgtagtt taaagaaagg accttttgtc gatgagcatt taatgaaaaa
    10381 agttgaagct caagcagggg ttgaaaagaa aaaagttatc aaaacttggt cacgccgttc
    10441 aacagttttc ccaagttttg ttggatatac catcgcagtt tatgatggac gtaaacatgt
    10501 accagtttac atccaagaag acatggtagg acacaaatta ggtgaatttg cacctacaag
    10561 aacttatcgt ggacatgctg ctgacgataa gaaaacgaaa cgctaatttg aggggaggat
    10621 aaatccaaat ggcagaacaa atcacatcag ctaaagcaac tgcaaaaaca gttcgcactt
    10681 cacctcgaaa agcccgttta gtaattgacc ttatcagagg taaaagtgtc gcagatgcaa
    10741 tttcaatctt gaaattcaca ccaaacaaat ctgctggaat cattgaaaaa gtattgatgt
    10801 cagcagttgc gaatgcagaa aataactttg acttagatgt tgaaaacttg gtagtaagcg
    10861 aagcttttgt taacgaagga cctacaatga aacgtttccg tcctcgtgca aaaggatcag
    10921 cttctccaat caacaaacgt acaagtcata tcacggtagt cgtatcagaa aaataaggag
    10981 ggataatcag tgggtcaaaa agtacatcca attggaatgc gtgtaggcat catccgcgac
    11041 tgggatgcta aatggtatgc tgaaaaagag tatgctgagt tcttacacga agatttaaga
    11101 atccgtaaat ttatttcaac taaacttgct gatgctgctg tatcaacagt tgaaatcgaa
    11161 cgcgcaacag gtcgcgtaaa catttcaatt cacacagcta aaccaggtat ggttattggt
    11221 aaaggcggtt ctgaagtcga aagcctaaga aaagaattaa acaaattaac tggtaaaaga
    11281 atccatatca atatcgtgga aatcaaaaaa ccagatctag atgcaaaatt agtaggtgaa
    11341 ggaatcgcac gtcaattaga aaaccgtgtt gctttccgtc gcgctcaaaa acaagccatc
    11401 caacgctcaa tgcgtgctgg cgctcaaggg atcaaaactc aagtatctgg tcgtctaaac
    11461 ggagcagata tcgctcgttc agaaggatac tcagaaggaa ctgttcctct tcatacttta
    11521 cgtgcggaca ttgattacgc atgggaagaa gcagatacaa catacggaaa actaggagtt
    11581 aaagtgtgga tctatcgtgg agaaattctt ccaactaaaa aaaacactga gaaaggaggg
    11641 aaataatcat gttagtacct aaacgtgtaa aacaccgtcg tgaattccgc ggaaaaatgc
    11701 gcggggaagc taaaggcgga aaagaagtag cattcggtga atacggtttg caagctgttg
    11761 attcacattg gatcacaaac cgccaaatcg aagctgctcg tatcgcaatg actcgttaca
    11821 tgaaacgtgg tgggaaagta tggattaaaa ttttccctca caaatcttat actgccaaag
    11881 caattggggt acgtatgggt tctggtaaag gggcacctga aggatgggtt gcaccagtaa
    11941 aacgtggtaa aatcatgttt gaaatcgcag gcgttcctga agaagtagct cgtgaagcgt
    12001 tacgtctagc ttctcacaaa ttaccaatga aaactaagat cgtaaaacgt gaggaaatgg
    12061 gtggtgaatc gaatgaaggt taaagaaatc agagaattaa ccactgccga aatgcttgat
    12121 caagaaaaac aattaaaaga agaattgttt aatcttagat tccaattagc aacaggtcaa
    12181 ttagaaaaca ctgcacgtat taaagaagta cgtaaatcga ttgcacgcat caaaactgtg
    12241 ttgcgtgaac aagctaagta attatggaag gaggccatta agcgtatgac tgaaggaaga
    12301 aatcaacgca aagtttatca aggtcgcgtg gtttcagaca aaatggataa aacaattacc
    12361 gttgctgtcg aaacaaagaa aaaccaccct atctatggca aacgcatgaa ctattctaag
    12421 aaatacaaag tgcatgatga aaacaacaca gcaaaagttg gcgacatcgt gagaattatg
    12481 gaaactcgtc cattatcagc tacaaaacgt ttccgtttgt tggaagtagt cgaagaagca
    12541 gttattatct aataacacga gattttccta tatagattga atgcaaaatc tgaaaggagg
    12601 atacacaacg tgatccaagc agaaagtcgt ttaaaagtcg ctgataactc aggcgcacgt
    12661 gaaattttga cgatcaaagt ccttggtgga tctggtcgta aaactgctaa catcggtgat
    12721 gtgatcgttg ctacggttaa acaagcaacg ccaggtgggg ttgtcaaaaa aggtgacgtc
    12781 gttaaagccg ttatcgttcg aacaaaatca ggagctcgtc gtacagacgg ttcatatatt
    12841 aaatttgatg aaaatgctgc agtgattatt cgtgatgata aaagccctcg tggaacacgt
    12901 atctttgggc cagttgcacg tgaactacgt gaaaacaact tcatgaagat cgtttcacta
    12961 gcaccagaag ttttataatc ttgagacact tatcaaagga ggtgcgaaac agttatgttt
    13021 gttaaaaaag gcgataaagt taaagttatc actggaaaag acaaaaacaa agaaggcgtt
    13081 gtactagcag cgttcccgaa aaaagataaa gttattgtcg aaggtgtaaa tgtcgttaaa
    13141 aaacaccaaa aaccttctca agctgctcct caaggtggaa ttgttgagat ggaagcgcca
    13201 attcatgttt ctaacgtgat ggtggttgac tctactggtg tagctggccg agttggctac
    13261 aaagaagtag acggcaaaaa agtccgtgtt tctaaaaaaa ccggtgaagt tttagataaa
    13321 taaaagttta aggaaggagg gtcttactga atgaaccgcc taaaagaaaa atatcttaaa
    13381 gaagtaactc catcattgat ggaaaaattt aactatagct ctgttatgca aacacctaaa
    13441 gttgaaaaaa tcgtcatcaa catgggtgtt ggtgatgcgg tatcaaacgc taaaaactta
    13501 gataaagcag ttgaagaatt aactttgatc actggtcaaa aaccaatgat tacaaaagct
    13561 aaaaaatcaa tcgctggatt ccgtttacgt gaaggaatgc caattggtgc aaaagttaca
    13621 ttgcgcggag aaagaatgta cgaattctta gacaaattgg tttctgtttc actaccacgt
    13681 gtacgtgact tccatggtgt aagtaaaaaa gcctttgacg gtcgtggaaa ctatacttta
    13741 ggtattaaag aacaattgat cttcccagaa gtagattatg atttagtaga taaagtacgt
    13801 ggtatggaca tcgttattgt aacaacagca aactctgatg aagaatctcg tgagttgttg
    13861 acacaattag gcatgccatt ccaaaaataa ttaaaggagg cgaactacgt ggctaaaaaa
    13921 tcaatgattg ctaaaaacaa acgtcctgca aaattttcaa cacaagctta tactcgttgc
    13981 gaacgttgcg gacgtccaca ttcagtttat cgtaagttcc atctttgccg tatttgcttc
    14041 cgcgaacttg cctataaagg tcaaattccc ggcgtgaaga aagctagctg gtaaaaagta
    14101 aattcgcata agaaggaggt aaaagatcaa tggtcatgac agatccaatt gcagattttc
    14161 taactcgtat ccgtaacgca aacatggtta aacatgagtc tttagaagta cctgcatcaa
    14221 aaattaaacg tgacatcgct gaaatcttga aacgtgaagg tttcgttcgt gatgttgaat
    14281 acatcgaaga tgacaaacaa ggcgtgatcc gtgttttcct aaaatttggt aaaaacggcg
    14341 aacgtgttat cacaaactta aaacgtatct ctaaaccagg tttgcgtgct tatgtcaaag
    14401 ctgacgaagt accaaaagta ttgaacggtt taggtatcgc aatcatttcg acttctgaag
    14461 gtgttgtcac tgataaagaa gctcgtgaaa aaaatatcgg cggcgaagtt atcgcttacg
    14521 tatggtaatc aagtagaatc tacaaggagg tgtctttaag tgagccgtat cggaaataaa
    14581 gtcgttgtta tccctgaagg agtaacagtt actcaagacg gaaacaacgt tacagttaaa
    14641 ggaccaaaag gtgaattgac tcgtactttt tcagcagata tcaaaatgaa tatcgaagga
    14701 aacgaagtaa ctttcactcg tccaaacgac agtaaagaaa tgaaaacaat tcatggaaca
    14761 actcgtgcga acttcaacaa catggttgta ggtgtcagcg aaggtttcca aaaagcgtta
    14821 gaactaatcg gggttgggta ccgtgcacaa ttacaaggat caaaacttgt attgaacgtt
    14881 ggttactcac atccagtaga aatcacacca ccagctggag taactgttga agtaccagct
    14941 aacacacaag taattgttaa aggtgcgaac aaagaagaag ttggcgaatt agctgcaaac
    15001 attcgtggaa ctcgtcctcc agaaccttat aaaggtaaag gtattcgtta tgttggcgaa
    15061 tatgtacgcc gtaaagaagg taaaactggt aaataatcgt ttagaaaaat tgcatagaag
    15121 ccagcagcag gaaaaaggca tttggctgaa gtttaccttc aaaaaaatac atttttactt
    15181 gtgccgcagc ttctattttt cgaaacgtat ttactgcttg aaatagtagt acaataaaaa
    15241 taaagaggtg acaattgtga ttacaaaacc agataagaac aaaacacgtc aaaagagaca
    15301 tcgtcgtgta cgtaacaata tctctggtac tgctgagcgc ccacgcttga acgtttttcg
    15361 ttctaacaaa aacatctacg ctcaaattat tgatgacgta gcgggtgtaa cgctagcaag
    15421 tgcctctacc ttggataaag aaatttcagg tggaacaaaa acagaagcag cacaagctgt
    15481 cggtaaacta gttgctgaac gtgcagctga aaaaggcatt aaagtagtag tctttgaccg
    15541 tggtggatac ctttaccatg gccgtgtgca agctttagct gaagctgctc gcgaaaatgg
    15601 actagaattt tagaagaagg aggaatacca ttcatggttt atatcgatcc aaaacatttg
    15661 gaattagaag accgcgttgt tgcaattaac cgtgtaacaa aagttgttaa aggtggacgt
    15721 cgtctacgtt ttgctgcttt agttgttgtc ggtgacaaaa acggacacgt agggtttggt
    15781 acaggtaaag ctcaagaagt tcctgaagca atccgtaaag caattgaaga tgcgaagaaa
    15841 aacttagttg aagtacctat ggttggttca acaatcccac acgaagtaat cggtgctttt
    15901 agtggtggta gaatcctaat gaaacctgct gtagaaggtt ctggggttgc tgctggtgga
    15961 ccagttcgtg ccgtattaga attagctggg gtagcagata tcacttctaa atctttaggc
    16021 tcaaatacac caatcaacgt tgttcgcgca acagttgaag gtttgaaaca attgaaacgt
    16081 gcagaagaag ttgcagaact tcgcggcaaa tcagttgaag aattaatcgg ataaggagga
    16141 caacaataat ggctgaatta aaagttactt taaaacgcag tattatcgga cgtcctcaaa
    16201 accaacgcga tacagttaaa gcgttaggtc ttacaaagat taacagttca gtggttaagc
    16261 cttctaatga agcaattaaa ggcatgatca atactgtttc tcacctagtg gacgttgaag
    16321 aaatttaatt tagaaaaatc ataactagaa cattaaggag gtgccaaacc tatgaaactt
    16381 catgaattaa aacctgctga aggttcacgc caagtacgta accgtgttgg tcgtggtact
    16441 tcatctggta acggaaaaac tgccggacgc ggacaaaaag gacaaaaagc tcgttcaggc
    16501 ggcggtgtgc gtttaggatt tgaaggggga caaacaccat tgttccgtcg tttaccaaaa
    16561 cgaggattca caaacgtgaa ccgtaaagac tatgcagtca ttaatcttga tgtattgaac
    16621 cgctttgaag acggagctga agtaactcct gttgctttag ttgaagctgg aatcgtgaaa
    16681 aacgaaaaag ctggaatcaa agtattagct aatggggaac taaacaagaa attaactgtg
    16741 aaagcagcaa aattctctaa agcagctcaa gaagcaattg aagcggctgg tggatcaatc
    16801 gaggtgatct aatgtttaga ctattaaaag atgcttttaa agtaaaagac attagatcga
    16861 aaatcctatt tactgtattt gtgttgtttg tatttcgcct aggtgcacat attactgtac
    16921 caggcgtaaa tgctgcttca ttgcaaaacc tttcttcttt gccatttttc aacatgttga
    16981 acttggtaag tggtagtgcg atgcaaaact tttccatttt ctcaatgggg gtttcaccat
    17041 acattactgc ttctatcgtc attcagcttc ttcaaatgga catcgtgcct cgatttgttg
    17101 aatggtcgaa acaaggggaa gttggtcgta aaaaactgaa tcaagcgact cgatatctga
    17161 cattagtatt ggcatttatc caatctgtcg gtatcactgc tggattcaat caatacgctc
    17221 aattaggttt cgtgaataat cctaacgtag caacttacgt aacgatcggt ttgattctaa
    17281 cagccggtac gatgtttgtt acttggctag gagaacagat cacagaaaaa ggtatcggaa
    17341 atggcgtatc aatgatcatt tttgcaggta tcatttctag attgcctgaa agtatcagag
    17401 aaatttatga agattacttt ataaatattg atccttcaga catatggaaa tctgctattt
    17461 ttgtggccat tttactagta gcggtcttgt tgatcgtgac attcgtaaca tttttccaac
    17521 aagctgaacg caagatcccg atccaatata cgaaacgcgt agctggcgcg cctacaagca
    17581 gctatctgcc gttgaaagta aatgcagctg gagttattcc agttatcttt gctagttcgt
    17641 ttattgctac gccaaatgct gttttacaag cattgagcgg tagattcggt tctgaaaact
    17701 ggtacaaagt gatgatggag atctttaact ataatacagc acctggtgct acgatttata
    17761 cgattctgat cgttgccttc actttcttct acgcctttgt tcaagtaaac cctgagaaat
    17821 tagcggaaaa cttgcaaaag caaggaagtt atataccaag cgtacgccct ggtaagggga
    17881 ctgaagagta tgtctcaaga ttactgatgc gtttaagtac agttggtgca gtcttccttg
    17941 gtttagtggc attgcttcca atcattgctc aaatggtttg ggatcttcca caatcaatcg
    18001 gattaggcgg tacaagctta ctgatcgtta tcggtgtagc tttagaaact gcgaaacagt
    18061 tagaaggctt aatgctgaaa cgtaaatatg ttggattcat tgagtaagaa agcgtgttga
    18121 ggtttcccct caacacacgt tctcacacat ttattaggag ggaaaacaat gaacctcatt
    18181 ttaatggggc tgccaggcgc aggtaaagga acacaagctg aaaaaatcat tgatgcttat
    18241 ggtattccgc acatctcaac aggagatatg ttccgcgcag cgatgcaaaa cgaaacagct
    18301 cttggcctgg aagcaaagtc ttatatggat aaaggtgcat tagttcctga tgaagtaaca
    18361 aatggaatcg tgaaagagcg actagctgaa ccagatacag agaaaggctt cttattagat
    18421 ggctttccac gtacgctaga tcaagcaaaa gctctggacg caatgctaaa agatttgaat
    18481 aaaaaaattg atgctgtcat cgatatccat gttggtgaag aaatcttggt cgaacgtcta
    18541 gccggccgat ttatctgcag caattgtgga gcaacttacc ataaaatttt caaccctaca
    18601 aaagttgaag acacatgtga tcgttgcggt gggcatgaat tctatcaaag agaagatgat
    18661 aaacctgaga cggttaaaaa tcgtctggct atcaacatca aaaatagtga accaatcttg
    18721 gcttattata aagaacaagg tttgctaaac actattgatg gtgctcgtga aatcgatgct
    18781 gtatttgcag atgtacaaaa aatcattgaa aaatagcacg attgcccgta gccgacttgc
    18841 attatgcagt tactcttgtc aagttcaata aattatgata gaatatgtca gtccgacttc
    18901 tagagaatcc tctagatgat ccattttgag aattttgagg tgggaactgc agtttccgcc
    18961 attttatcgt gtgaaaatgc gaaacaagta caaggaggta ctgtgcgtgg caaaagaaga
    19021 tatgattgaa gtcgaaggta cagtcgtcga aactttgccg aatgcaatgt ttaaagttga
    19081 actagaaaac ggacaccaag ttcttgctac tgtttcaggt aaaattcgta tgcattacat
    19141 ccgtatctta cctggtgaca aagtaacggt tgaattgtct ccatacgatc ttaaccgtgg
    19201 ccgtattact tatcgcttta aataattgta ctccgtaaaa ctacaggagg tattatcatg
    19261 aaagtaagac catcagtaaa accaatgtgc gaacattgta aagtaattcg tcgtaaagga
    19321 cgcgttatgg tgatttgccc agcaaatcca aaacataaac aacgtcaagg ataattggag
    19381 gtgctataaa tatggctcgt attgcaggag tagatattcc tcgtgataaa cgtgtagtag
    19441 tttcacttac ttatatctat ggtattggta acactactgc taaaaagatc ttagctgatg
    19501 ctggtgtatc tgaagatgtt cgcgttcgtg aattaacgaa tgaacaaaca gatgctatcc
    19561 gtgcagaagt agataaatta aaagttgaag gggatcttcg tcgtgaagtg aacttgaaca
    19621 tcaaacgttt gatggaaatc ggttcttacc gtggaatccg tcatcgccgt ggattgcctg
    19681 ttcgtggaca aaacacgaaa aacaatgcac gtactcgtaa aggcccagct cgcgctatcg
    19741 ctggcaagaa aaaataattc ttaagtgaag gaggttaaaa cttcatggca gcaaaaaaag
    19801 tgaatcgtaa acgccgtgtg aagaagaata tagaagcagg tattgcacat atccattcta
    19861 cattcaataa cactatcatc atgatcactg atactcacgg aaatgcatta gcgtggtcat
    19921 cagctggttc attaggtttc aaaggaagca aaaaatcaac tccttttgcc gctcaaatgg
    19981 cagcagaaac tgctacaaaa gcagcaatgg aacacggatt aaagactgtt gaagtaacag
    20041 ttaaaggacc tggttctgga cgtgaagcag caattcgttc attacaagct acaggtttag
    20101 aagtgactgc aattcgtgac gtgactccag ttcctcataa tggtgcccgc cctccaaaac
    20161 gccgtcgtgt ttaatgagtg ccgctcattt tgagttttat cagtaacgtc attgaacaag
    20221 acacttttcg ttttgaaagg ggtaaagata agaatgattg agttcgaaaa accaagaatc
    20281 gcaaaaattg atgaagaaaa agattatggc aagttcatcg ttgaacctct tgaaagaggg
    20341 tatgggacta ctttagggaa ttccctacgt cgtattttat tatcttcttt gccaggtgct
    20401 gccatcacta atattcaaat cgatggtgta ctgcatgaat tctctaccat caaaggtgtt
    20461 agagaagacg tcactcaaat catcttgaac attaaaggct tagcgctgaa aatgtatggc
    20521 caagaagaaa aaacccttga aatcgatatt accggacctg ctacagtaac tgctggcgat
    20581 attatcgttg atagtgatgt agaaattctt aacaaagata tgtacatctg tagtgtctct
    20641 gaaggagcta cgttccatgc tcgcttaaca gtgaaacctg gacgtggtta tgttcaagca
    20701 gatgaaaata aaaaagaaga tatgccaatt ggcgttcttc cagtcgattc aatctacaca
    20761 cctgttcgtc gtgtgaacta tcaagttgag aacacacgtg ttggacaccg tgaagatttt
    20821 gataaattga cgatggagat ttggacagat ggttcaattg aaccgttgga agcaatgagt
    20881 ttgtctgcga aaatcatgac tgaacatttg gatatttttg tgaacttaac tgatgaagca
    20941 aaaaatgctg aaatcatggt cgaaaaagaa gaaacacaaa aagaaaaaat gttggaaatg
    21001 acgatcgaag agttagactt gtctgttcgc tcttacaatt gtttgaaacg tgcaggaatc
    21061 aatactgtac aagaacttac aaataaatct gagcctgaaa tgatcaaagt ccgtaactta
    21121 ggacgtaaat cattagaaga agttaaagca aaattgcacg acttaggttt aggtttacgt
    21181 aaagaagatt aaaacctttt acgaaggagg gaaaccaacg tgagttatcg taaactagga
    21241 cgcacatcta gccaacgtaa agcgatgttg cgtgatttaa caactgattt attaatcaac
    21301 gaacgtattg tgactactga agctcgtgcg aaagaagttc gctcaactgc agaaaaaatg
    21361 atcactttag gtaaacgcgg agatttgcat gcacgtcgtc aagctgcggc tttcgtacgt
    21421 aatgaagttg ccagcgtgcg tgaagaaaat gaggacatcg tcattgaatc agctttacaa
    21481 aaactgttca acgatattgc acctcgttac gcagaacgtc aaggcggata cacacgcatt
    21541 ttgaagacag aaccaagacg cggtgatggt gcaccaatgg ttgtcttaga acttgtctaa
    21601 aatcacacta ataacatcac tttatagatg attcttttag agtgttatga tgttggaaga
    21661 tatttttccg agtctagctc agcgctgctc ctcaagcgaa tatcgcttgg ggagtaagca
    21721 ctcatcataa agtgtttttt tataccttca attacctgtc atgacagata tgtttgaagg
    21781 cataaaaaaa cattttaagc gagaaataat tgaaaatttt gtcttttttt gatagtgtta
    21841 gtaggaactt taggatgaag aaggagtaat catggagcca attattgaat tagaaaagat
    21901 aaattataaa taccagccag atgatcttcg ccccgcattg aaagatgtct cctttacaat
    21961 tgataaaggt gagtggatcg ctattattgg acacaatggt tcaggaaaat cgacacttgc
    22021 caaaacgatc aatggattgc ttctgccaga atcagggatc gtgaaagtgg gaaatcaaat
    22081 attggatgaa gaaaatattt ggacgatccg acagatggtt ggaatgattt ttcaaaatcc
    22141 agacaatcag tttgttggtt cgacagtaga agatgacgtt gcttttggtc ttgaaaatca
    22201 agggattccc agagaagaaa tgctagtgcg tgtgaaagat gcacttgaga aagttagaat
    22261 ggctgagttt gcttcacgcg agcctgctcg tttatcagga gggcaaaaac aacgtgttgc
    22321 cattgccggc gtggttgcct tacgacctga tatcattatt ttagatgaag caaccagtat
    22381 gctagatcca gaaggtcgtg aagaagtgat ctctacgatc aagaagatca aagaagaaag
    22441 tcaattgacc gttatatcta tcactcatga tattgatgaa gcagctaatg ccaatcggat
    22501 actagtgatg agacaaggcg aacttgtccg tgaaggtaca ccaaaagaaa ttttttctgc
    22561 tggtcctgaa ctgatcgact taggtctaga tctaccattt cctgaaaaac tgaagagcgc
    22621 actgaaagaa cgtggggttg atgtgcccag tgaatatatg acagaagaaa ggatggtgga
    22681 ttggttatgg acatccgttt tgaacaagta gacttcactt atcagccgaa tacaccattt
    22741 gaacaaagag ccttatttga catcaatatg acgatcaaag aaaacagtta tactgcatta
    22801 gtagggcata ccggaagcgg gaaatcaacc ttacttcagc atttgaatgc gcttgttaag
    22861 ccaacaagcg gtacagtcca tattggagaa cgagatattc agccggatac ggataataag
    22921 aatttaaagc ccatccgaaa aaaagttggc atcgtctttc agtttccaga agcacagcta
    22981 ttcgaagaaa cggtagcaaa agacattgct ttcggtccta aaaactttgg tgtcagcgaa
    23041 gaagaagcac tagtcctagc aaaagaaaca ttagaacaag ttgggctgga tgaaagctat
    23101 ttggaacgtt cgccatttga actttcagga gggcaaatgc gtagagtagc aatcgctggt
    23161 gtgcttgcca tgaggccgga agtacttgta ttggatgaac caacggcagg acttgatccc
    23221 cagggcagaa aagaaatgat ggagatgttt tggcgtcttc ataaggaaca gcagatcact
    23281 attgtattag ttacgcattt aatggatgat gtagccaatt ttgcagatta tgtctatgtg
    23341 ttggaaaagg gacgaatcgt taatagcggg gagccgcaag aggtctttca aaacattgaa
    23401 tggctaaaag aaaaacaact aggcgtacct actgccaccg agtttgcaga agaattaatg
    23461 gctaaaggga tgaactttgc taccttgcct ttgacagctg aagaattagc agacgctatc
    23521 gtccattatg ctggaggaaa tgcccatgat gaataaattg atatttggtc gttatatgcc
    23581 tggagattcc ttgatccatc gattggatcc ccgagctaaa ttattagcca gtttttattt
    23641 tatcggaatc atttttttag caaataattg ggaaacgtac cttttgttgg catttttcac
    23701 attactttct gtcttcttgt caaaagtaga tttgaaattt tttatccgag gcattcgccc
    23761 attgatctgg ctaatcctct tcactgtagc tttgcaggtc ttgtttacaa gtggcggaga
    23821 aacttattgg agttggggtg ttttccatat cactgaattt ggtgtgatga acggcctctt
    23881 tattttctgt cgattcgtgt tgatcatttt tatgtcgact ctactcacgc ttactacacc
    23941 gcctttggaa ttatcggatg cgattgagta cttgcttcgt ccattgaaag ccgtacggtt
    24001 cccagtccac gaaatttcat tgatgctgtc gattgctctt cgttttgtgc ctacattgat
    24061 ggacgaaaca gaaaagatca tgaacgctca gcgcgctcgc ggtgttgatt ttggtgaagg
    24121 aaacctagtt cagaaaatga aagcagtcat tcctttgctg attcctttat ttgtcagcag
    24181 cttcaaccgt gcggaagatc ttgcgactgc tatggaagca agaggctaca aaggtgggga
    24241 aggaagaacg aaatatcgtg tattgcattg gcataataga gacacgatgg ttatcattgc
    24301 ttttgtatta ttaactgtgc tactgatctt tttaagagga taaacgttca gtgggccgct
    24361 atctattctt ttcttgctga tctgtttgca agaaaggatg caagcagtgt atgacaagaa
    24421 atattagggt ctgtggcaga ctaaggtcac aggcctcttt cttttatctg aaaagaaaga
    24481 acgaaaaaat tttatagaaa aatcattatt ccagataacc ttttaggaac aaaggagaga
    24541 aaaacatggt acgttacaaa gcaatcattg cgtacgacgg aacaaatttt aatggattcc
    24601 aaaaacagcc aaatgggcgt actgttcaag aagaagtaga aaaaacatta caaaaaatgg
    24661 caaatggaaa agaaatcacg gtctttggtt cgggaaggac agatgccgga gtacatgcga
    24721 tcgggcaagt aatccatttt gattatccag aggagcgtcc gttagaacga atgcgtttcg
    24781 cgctagatac ccagtcacca gaggatattg ctgtaagaca agtagaaatt gtttcagaag
    24841 acttccacgc aagatatttg gtcaaagaaa agacgtatca atttcgagtg gatataggga
    24901 aaccacgcag tccttttcga cgccattatg caagttattt tccttaccca cttgatttgg
    24961 aaaaaattca gcgggcgctt cctgatttat tagggactca cgatttcacc tctttttgtg
    25021 catcaggaag ctcgatagag gataaaatac gtacgatcta cgaggctaag atggaggtaa
    25081 atgaaacaaa agacgaattg ttgtttacat ttaggggaaa tggcttcttg tataagatga
    25141 tacggatctt agtaggaacg cttttgaaaa ttggcaatgg ccgattgcct gaagatagta
    25201 tcccagatat tattgcgaaa aaagatcgta atgcagcagg acctacagca caccctgaag
    25261 gactttattt gtatgaagtg aaatatgaat aagaggttat gatagaagtg gtcagcttca
    25321 agaaataaga aggtatttac gaaaattact tctcaaaatt ttgcaaaatt tcggcttatt
    25381 ttcgaagaag ctacttctgg aacaccgttt aagaaagagg tcgtgagcct gtcatttaac
    25441 tctgagtcac aaagagcaat ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact
    25501 tgtggacgag gcgttgataa gtgaatcatc tttttgttac ctattttttc tgtaaccaaa
    25561 aaacgatgga acgttgtttt gattgccctt ggtgcggaca gtctgaacag gttccatcgt
    25621 ttctttattt tctacgtctt cttctgtgtg tgtttcgtgg ttgttcttct attgctggat
    25681 caatcgtgcc ataaaagggg cgattgcggt caaggtacca gacataaaga ataaacaatg
    25741 caatacatag gacaaaaagg attgcaccta tcgtaaatcc ttcaggcaag taactgaaag
    25801 tgacagtatg tttacctgct ggaactttta tcatcaagaa tgcgtctttg aaattgctga
    25861 ccttgactgg tttcccatca attttagctt tccatccttt gtcataagga atagttgtca
    25921 ccaacatgga atcttcagcg gtatttacag tcccagaagc ttttctgcca gtggcttcca
    25981 tttcgacacc tttttctttg atctgattca ttgcggcttg ataggctaca acatctagac
    26041 caacgacttt aggctccatg aaacttattt ctttcgtccc ataaaaactt gcagtaaatg
    26101 tcactgttgt ctcttgagga taatagccaa gatcatagta ttgaccagaa atgttgattt
    26161 gcgtttttcg cgatgtacca ttgacagtga tcgtcgcact agaactttct aattgcgcaa
    26221 aattggttgg gaacaaactt aagtaagctt gtgtatttgc aggcacatgg acttcccaag
    26281 tgagctcttt tggctggtta tctgctgttt ctgagtaagt gacgccagtc gttgtctttg
    26341 tgatcgtcgt attcaactga ttcttcaaag taggtgtata gaatgtaaaa tactgttcat
    26401 ttagaccaga caatgtattg atcaagtttg tctgactggt caaattatct gtcactggct
    26461 gctcaacatc attaacggac aacggtgcag taaatgctag tggcaatgca ttgacattct
    26521 gatacagtgt atagtcgccg ctttgaccag cttggcggaa gccatattta ctgaacgtgc
    26581 tttcgttttt agaaatgtta tatttgattc ctgtaaaccc atccatcagc aaagtattat
    26641 tgttataacg gatattcaaa tttgtccctc ttgaacggaa acctaattta tccaggtaag
    26701 aggaagaatt acgatttctt atagaagaaa acaaactgat accactgtaa ccatagttga
    26761 tcgaatcgtt tgcagatact ggatccagat tttccaagcg gtaaaacgag tcggaatctt
    26821 tttttgtttc atttacaaga gctttcaagt caggataagg atcagtatat aaactgcggg
    26881 aagcataatt ccaatcatca agaatgccat ggatcatact gttcgtattg accagtgctt
    26941 cagcagacat gatcaataat aacaatatac tcagatgatt gaatgaaagt tttttatgct
    27001 gataaaatcc cacagcaaaa gcataaatcc ctaagaaaac cactgtcaat acaaaggaag
    27061 tcatcgtcac ataggtgtag cttccttcag gtttgatgcc ccatgccacc gcaaagagag
    27121 cagctacgac caaaatgatt cctaacaaca gcccaccatc gtcttgttct atcttttccc
    27181 atccttttcc tgcaagcagg agcactaaga aagagaacag ataggcataa cggaataaaa
    27241 acatattcgg tgcatgcata ccatgccaga acaaattcaa tggaacaaga taaaaacttg
    27301 cgataagtat ggcgaaaaga ctgccgtaca aaaatttatt tttcaacggt actttccgac
    27361 tggcaaaata aaacagacag aggactaatg gtagcagacc tgcgtaaacg aacggaatcg
    27421 atccgtattt tgtcgtgtca taaacaccaa tcatattttt catgattaga tccagataag
    27481 aagtcgcttc tgttttcaac gtagtgatct gactcagtgc ctcgccattt gaccgaagat
    27541 ccagcactgc tggcaataca atgatcattg aagcgccgcc tgccagtaaa gaagtgatac
    27601 cataaggaac aatcgttttt ttatattctg accagtttgt caatagacgg gcgataaaat
    27661 ataagaaaga aaatacgcca atcataaaac ccatataaaa actggataaa aacaagagta
    27721 aataactgat aaataaaagt ttcggccgtt taagatccat aacacgatga atccccaaaa
    27781 tcactaatgg aagataaaca aatgcatcca gccacatgat gatctccgaa tgtgcagtga
    27841 taaatgagat caatgcgtaa ctgacgctaa gtgcaacatg cccccagcga ggaatcttga
    27901 acgtacgtgc aagaaaccaa aaagccaatc cggcacagcc gaccttaagc aaagtcagaa
    27961 catacagtgc atctggcatc atctggtttg gaaaaaagaa caccagtggc gtgaagatcc
    28021 cgcctagata gtaagaaatt aaagataaat aattcaatcc tagagaagca ttccatgtat
    28081 aaaaaatact ttgctttcca agaagaacat ttcggaagct ggcatgaaaa ttagaaaact
    28141 gtgaaaaggc atcacttgcc aaaatacttc tgctgcttcc aggatagatt ccgatactta
    28201 gataaacaag tgctaaaatc aaaaacggaa tcaggaaact agctgctgcg tagcgtccgt
    28261 tttctctaaa gaatgacttt aaattttttt tcatatttat tttccaccaa atttctatga
    28321 agttgtattc agtttatcat atccttgttt ttctctgctt aaaaagagtc tagttttcac
    28381 aaaaaaataa aatttagaat aaataacttc ataatttgtt cgtataacaa aaataaacgc
    28441 aaaaaaaggg taggaatcaa aagtcccacc cttaaaaatt tttattaaat aaaaaaccgt
    28501 tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt cctgatttca
    28561 aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg cgaacttttt
    28621 gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg ttgtttccac
    28681 tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc tcctttgcct
    28741 tgatttggag caatagaata attcagctca taccagacta acatatcata tagaatggga
    28801 atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt gtaaattaaa
    28861 caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg ccttttattt
    28921 caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac ccgttaagga
    28981 ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat taccaatgat
    29041 gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt aggaatggcc
    29101 agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa ttactcaaga
    29161 ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac gattatcagc
    29221 tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa atacaatcta
    29281 gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg tgttcgtgta
    29341 ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag gaggattatc
    29401 taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca ggcaggtgcg
    29461 aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc tgtgccagat
    29521 ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc agtagtagat
    29581 tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt gctgatgggt
    29641 gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc taagcaaatt
    29701 ccagatgttg tctcattaaa tgcttctgat cttgctgcag ccttcactca tggagttgaa
    29761 actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt agctagagaa
    29821 gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga agtaatgaca
    29881 gctgttgtaa aaggagcaaa gcgtgcttta gctaaaactc cagacttgct tccagtccta
    29941 aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta tgagggcttt
    30001 ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc tccagcagaa
    30061 atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat ggcgacagaa
    30121 gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg acctacagtc
    30181 gatagtacat tcgattacga agaattccgt aactatttag atggtatagg agattcttta
    30241 cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa cccaggagaa
    30301 gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga caacatgcgc
    30361 ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc gaaaaaagaa
    30421 ataccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt ccaagagctg
    30481 tttgaaagtt taggcgctaa ttatgttatc agtggtggac aaacaatgaa tccaagtacg
    30541 gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt cttgccaaac
    30601 aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat cccagtagca
    30661 gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt caatgatcaa
    30721 gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt tgtgagcggt
    30781 caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa agaaggagac
    30841 tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac agcaacagcg
    30901 ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat cttggcaggt
    30961 gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga aaaacatcct
    31021 gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct attttctgct
    31081 gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa aggccttcgg
    31141 ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaaaggagg gaggaaaaat
    31201 ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa aaaagagcag
    31261 ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat tatccttttc
    31321 gctacgaaga tattcaggaa agaaatctaa acgagatcca ggatcaagaa aaggttactt
    31381 tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa aaaagccgat
    31441 tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt aatcaacctt
    31501 atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa tgggatgcaa
    31561 agcgtaaagc cttgaacggg atgaagattt taggttctca aacaatggat gacttttcgc
    31621 cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta atcaaaaaag
    31681 ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt gtggagaaat
    31741 atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa gaccacgaag
    31801 aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt caaatgcgtc
    31861 ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc ctttatgatg
    31921 tccaaaaatt gaaacaattt actcaaaagc taccttttga actgacaaat gcacaaaaac
    31981 gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag cgacttttgc
    32041 agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac gctaccgtta
    32101 cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa caacatatgg
    32161 aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgatt acggggtcaa
    32221 cgaaagcaaa agaacgacgt gagctccttg aaaaactaga gcaaggggaa attaacgtcg
    32281 tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta ggtctagtta
    32341 ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt gaaaaaggct
    32401 ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta gctattacag
    32461 catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt atcccgatcg
    32521 aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg aaaaaagaac
    32581 tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaagaatca gaagcattag
    32641 atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca ccaaaatatg
    32701 aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc atggaagcct
    32761 tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta ggggtaaacg
    32821 tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg gcacagctgc
    32881 accaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc ttagttgcta
    32941 atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc accaatggtt
    33001 ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt ggcttccgtc
    33061 aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat gttttggagg
    33121 tagccagaga agaggctgca gccctttggc aaataaagga ttggcagatg cttcctgagt
    33181 atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat ttcgattagc
    33241 agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg aaaattgccg
    33301 tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg gctttagcgc
    33361 agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa atcaagaaat
    33421 acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc aatagtgatg
    33481 atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt gctgcacaag
    33541 cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc gcgctactag
    33601 cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc ttgatgtcta
    33661 ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt ggcgctaacg
    33721 cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt tatgcacgta
    33781 aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca gaagcaacaa
    33841 aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca agcttgaatt
    33901 ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta gtggtcacag
    33961 atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg aatatgatga
    34021 atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc gctatgcttt
    34081 taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat ggcggtgccg
    34141 ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc cccgatgctg
    34201 ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt ggacaattag
    34261 tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcgat ccgttccatt
    34321 gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg taggtaattg
    34381 acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt taacaaagta
    34441 gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga tgaattgaac
    34501 atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt agagcttgaa
    34561 gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac agtcggtggc
    34621 gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa gaaggataaa
    34681 aagctgcatg cagcttttta tccttctttt tttaatcttt ttgacacgta aaagtcattt
    34741 ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcggt tttctatata
    34801 attattaata attcattaca caaagataag aataaccttt gttataaaac gatttattct
    34861 gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag gggtattcga
    34921 ttgtccgata accaattatt agaagttcag aacctacata caggattccg actcaaagat
    34981 gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga gattttagca
    35041 atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat gggattactt
    35101 gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa tttaatcgac
    35161 ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat tttccaagat
    35221 ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga aggactttcc
    35281 tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt gctagaacaa
    35341 gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact atcaggcggt
    35401 atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat cgtcattgca
    35461 gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga tttactgaaa
    35521 gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt aggagtcgta
    35581 gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga agtagcaggt
    35641 gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact gaactctata
    35701 ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt tccttctctg
    35761 aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat accagcaagc
    35821 gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt acgttgctcc
    35881 tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac agaattaatc
    35941 acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa tcgagtaacg
    36001 gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa gacatatggt
    36061 ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt gggattagaa
    36121 aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg aagcaaccgc
    36181 aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat gtccagtttg
    36241 aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt tgaacggtta
    36301 agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg gatgccggaa
    36361 gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg tctaggagta
    36421 gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt atctgctctg
    36481 gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca agaatacggc
    36541 ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg cgacaatatc
    36601 gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat ttataatgac
    36661 ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga tgtagaaaat
    36721 agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca aaaccaaaaa
    36781 gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac tacacacaaa
    36841 gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga cgtgttttat
    36901 tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc caagcgatgc
    36961 cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg atcgaacaga
    37021 tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt tggatcggaa
    37081 atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt gctgacttga
    37141 tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc cttacttatt
    37201 tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc ttggataaga
    37261 ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc ggcttgatca
    37321 tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca caaacactag
    37381 gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt ctgttaccat
    37441 caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga agtgaagtaa
    37501 tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta ccgacgaata
    37561 aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct caaatggggt
    37621 atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt ggttatccgg
    37681 gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg atcacatcct
    37741 tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat atcatcatga
    37801 gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga atggatcgat
    37861 gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga tacgaatgat
    37921 tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc tagtggtttt
    37981 attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga tgtatgttag
    38041 tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa agttcattct
    38101 gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg ctagaaactc
    38161 tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg gattagggat
    38221 catctctggc tactacggag ggatcattga caacatcttg atgcggatcg ttgattttat
    38281 catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt ccagatacaa
    38341 tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag cccgattatt
    38401 ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa aaacaatggg
    38461 aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt cattaatcat
    38521 tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt tgactttctt
    38581 aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt atgcaagtaa
    38641 cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc ttattttagt
    38701 tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag atgcgcgcca
    38761 acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg atttttggat
    38821 ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt acagatacct
    38881 ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt ccaattgcaa
    38941 caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc gcaacggaca
    39001 gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat gcagctttta
    39061 tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc acagacgacg
    39121 gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa gtaaaagaca
    39181 acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc ccttacgaaa
    39241 tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt aacatcgtag
    39301 gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa aaaatagacg
    39361 acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa tcgggtggtg
    39421 gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc aaagatatgg
    39481 aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac atgagtaata
    39541 tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat ggtacgccta
    39601 aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa gcattgaact
    39661 ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc tataaagaaa
    39721 ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta ggattcaagt
    39781 taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca aaaatggctg
    39841 ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta ggtgaacgtt
    39901 tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc cagtcatacc
    39961 atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa ctattagatg
    40021 atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat ggaaataaac
    40081 taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg gcagattact
    40141 atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt cgtctgatcg
    40201 aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat gtttaccaag
    40261 ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca aatgctgcgt
    40321 tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat atcagttcaa
    40381 gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa caatatgcat
    40441 ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca gtcaacgacc
    40501 gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat gtttctgtta
    40561 cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt cctttttcgg
    40621 ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg atttcatgac
    40681 ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat ttatgcaaaa
    40741 atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac gaatttttca
    40801 caacttctcg tcaatcacta ggattattaa agctttactt accttttgac cgaaccattt
    40861 caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca taaaaggaat
    40921 gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca tcgtttttca
    40981 cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg agcatcgcaa
    41041 tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt tagaactgat
    41101 ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat tgacaaaaac
    41161 acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat gtcactttga
    41221 tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc gaccagcatt
    41281 gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg gattcgacac
    41341 gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg ctttttcaca
    41401 tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg atgtatcaat
    41461 cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt ggatcgaagt
    41521 ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat tagcagaaca
    41581 agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa gctttgtatt
    41641 tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc attgattttg
    41701 aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat attacagagg
    41761 ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga aaaatgccgg
    41821 atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa gtcacagtga
    41881 ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt gtaacccgcc
    41941 gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc agattaaaag
    42001 atatccaaga attgttctta gattctggat taggaaaaga atcgttctca atcatttctc
    42061 aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg atctttgaag
    42121 aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag aagctttttg
    42181 aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa gaacagttaa
    42241 ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa gaaacactga
    42301 ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa gattgggaca
    42361 acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct gagtcgatcc
    42421 aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc gatcgtctga
    42481 tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag acagaaggac
    42541 aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa gaatatcaaa
    42601 cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa gaaagcttga
    42661 tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat ctcataaaaa
    42721 cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca gaattaagag
    42781 atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg aagtatctag
    42841 aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag caatcggaag
    42901 tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa aagcaggcgc
    42961 agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa caaaacggga
    43021 aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta atgaatcaag
    43081 tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac tattttggtt
    43141 tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt attgtcggag
    43201 cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa acagctttag
    43261 gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa gccatcacgt
    43321 atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca atcaaaccaa
    43381 gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt atagggattg
    43441 ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac ctgctaggaa
    43501 cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg atccgctatc
    43561 aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc atgacaggtg
    43621 gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa ttgaaacagt
    43681 tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa aaagttcagg
    43741 agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt acacgaggcg
    43801 aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc ataaatgaac
    43861 ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa ctgcagcagt
    43921 ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca gatcttgaat
    43981 cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc gaccagatgg
    44041 aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat cttgctgtct
    44101 tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc caaaaagcag
    44161 aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca gcagactttt
    44221 ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta tctgctcagc
    44281 gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga aggcaaaagg
    44341 agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa gcacgactgt
    44401 cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta gataatcatt
    44461 tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa gactatcaag
    44521 aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa cagattgaaa
    44581 aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt gaaagacata
    44641 cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg tttgaaacga
    44701 tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa gctatccgtc
    44761 aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa ttagtactga
    44821 cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct cctggcaaaa
    44881 aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc atcgcgttgc
    44941 tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag gtagaagcag
    45001 cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc caagatgaca
    45061 cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat gtactgtacg
    45121 gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg gaagatatca
    45181 atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca aattagtagc
    45241 aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg agaataaaca
    45301 agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag gacgcccctt
    45361 agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg actacagcat
    45421 cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg aaaagaaagt
    45481 gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg atctgccact
    45541 agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga agcaatcctt
    45601 gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg atattacaga
    45661 tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag atcctaaaat
    45721 caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa gaagtatgct
    45781 tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat tggcaaaaga
    45841 tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg atttaccgat
    45901 gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg tcaaagaagc
    45961 agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga tccaaaaatt
    46021 tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt gcttttactg
    46081 gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt ttagaaaaga
    46141 cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga acagtcgatg
    46201 aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt tttgaaacag
    46261 cgatacgaat taccgaagca ttacgtcaag aagtgaaact cagaaatgcg aaaaaatcgg
    46321 cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg gaaggaatca
    46381 atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg tttgtcggtg
    46441 ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc aaaaacgaag
    46501 gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc gaccagttag
    46561 ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga ggcgatccag
    46621 cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat gttttactag
    46681 ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta gaaaaaataa
    46741 aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt gtattagatg
    46801 caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca acagatgtga
    46861 ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta cttgctatcc
    46921 gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc gatgacttgg
    46981 agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta aaagaagaat
    47041 aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt tgaatgattg
    47101 tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta agcggttctc
    47161 aaacaatttc gttctttttc ttagactttt tgattataat tagattcata tgaattggag
    47221 gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc ccttttatcg
    47281 accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca ggaaatctag
    47341 caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat atccaaacag
    47401 attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt cccgaattat
    47461 ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa gaagagatga
    47521 tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc attattgcaa
    47581 aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaagaa tacgggaagc
    47641 gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag ttgctaagaa
    47701 cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca gaactttatt
    47761 tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta ttcggtgctt
    47821 caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa cctgttcgca
    47881 gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt tcctatgctt
    47941 ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa ctttcggagg
    48001 agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca gatttagcaa
    48061 caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat gcaagattgg
    48121 gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg ttatggacag
    48181 aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat aacaaagtag
    48241 cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa attcttgaag
    48301 gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta gtagaggaag
    48361 cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat caagaagcac
    48421 agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat aaagaaagcc
    48481 atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact cagatattta
    48541 tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg gatcaatttt
    48601 tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg acaagtaagg
    48661 acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa gtcttaaaag
    48721 aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa gcggtaaaag
    48781 cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc aattacgggt
    48841 taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca ggtttagcca
    48901 ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga acggaatatc
    48961 gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct gctaatgtta
    49021 ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt gaccctttgc
    49081 gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta gaacaattga
    49141 tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa attgtttatc
    49201 taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact gatgaatttt
    49261 ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct aagattagtg
    49321 gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa aaagcatatg
    49381 ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct tttgcaggaa
    49441 aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta ttttaaaaga
    49501 aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt tttttgcatg
    49561 taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt ttttcttttt
    49621 tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt cgatatcaat
    49681 cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct caggatttgc
    49741 agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta gctggatata
    49801 ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat ctggatgttt
    49861 tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag caacagtcat
    49921 actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt cagggttcac
    49981 taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt gtgccataaa
    50041 aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc aataatacct
    50101 aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa gaatgaaaat
    50161 cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca ttattagtta
    50221 ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca gaactgctcc
    50281 agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga acgatttgtt
    50341 tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat ttgacccaaa
    50401 ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta ggtattttag
    50461 tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca gcattttgct
    50521 ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc cagaaaaatc
    50581 aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca gttgctactt
    50641 tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa gggaattttg
    50701 cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg ttgctcttgg
    50761 tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt gcagtaaatc
    50821 tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca ttggctagtg
    50881 cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt ccgaatcttg
    50941 tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct ttagcaggta
    51001 gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct tatgaaatac
    51061 cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt ctgataaggg
    51121 gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt tcttgtttta
    51181 tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt tgcatgtgca
    51241 ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag cagtgccaca
    51301 atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt gggacttgag
    51361 tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt gacgatgctt
    51421 cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg gctgagtcta
    51481 ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt actgatgtca
    51541 gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt catcatggat
    51601 ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa tatccagagt
    51661 cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt atttcgatat
    51721 cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct cgggatagac
    51781 acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg gacatcaacg
    51841 gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag ttatcgtgtc
    51901 ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc tatgctgttt
    51961 ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac agtgagtgtc
    52021 gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga aaggaagagt
    52081 atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca gtggttaaag
    52141 atgtacagtt aacgattcct gaaaagaaat tgactgcttt tatcggtcca aatggtgctg
    52201 gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca ggtgaaatct
    52261 atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa aaattatcga
    52321 tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta gtagaatttg
    52381 gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc gttcaggaat
    52441 cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact ctttcaggcg
    52501 gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat tatattttac
    52561 tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg cagattttaa
    52621 aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat atcaactttg
    52681 ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt acgcaaggaa
    52741 cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg gacatacgga
    52801 tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat taggagagaa
    52861 gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt agcagcatgt
    52921 ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca aactgaaact
    52981 acattgacga taacagatag taatggagac caaatcgagg tgccaaataa tccagaaaaa
    53041 gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt tggagataag
    53101 gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa aaaagtagaa
    53161 tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca gccagatcta
    53221 atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat tgctccgacc
    53281 attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa tgtcactgct
    53341 ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga attatctagt
    53401 gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact agtcacactt
    53461 gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt catccatgat
    53521 ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg tcaaagtgtt
    53581 tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga cagaacaaaa
    53641 gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt agcacaaacg
    53701 aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta tttaagcggg
    53761 ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt taaatagcat
    53821 aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac attcttaaat
    53881 agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt attgatgatt
    53941 ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca ttatctacat
    54001 catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat tttttcagca
    54061 tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga taaaacttaa
    54121 atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat ctgctgcttc
    54181 tcggcaataa g
//
LOCUS       DACCQI010000004        51626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCQI010000004 DACCQI010000000
VERSION     DACCQI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.004"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(1156..1554)
                     /locus_tag="IVO76_000157"
     CDS             complement(1156..1554)
                     /locus_tag="IVO76_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428153.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKELYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1770..2825
                     /locus_tag="IVO76_000158"
     CDS             1770..2825
                     /locus_tag="IVO76_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP7428154.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVIEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2845..3198
                     /locus_tag="IVO76_000159"
     CDS             2845..3198
                     /locus_tag="IVO76_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428155.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3380..3676)
                     /locus_tag="IVO76_000160"
     CDS             complement(3380..3676)
                     /locus_tag="IVO76_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428156.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3680..4498)
                     /locus_tag="IVO76_000161"
     CDS             complement(3680..4498)
                     /locus_tag="IVO76_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7428157.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4724..4945
                     /locus_tag="IVO76_000162"
     CDS             4724..4945
                     /locus_tag="IVO76_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP7428158.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4947..5213
                     /locus_tag="IVO76_000163"
     CDS             4947..5213
                     /locus_tag="IVO76_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7428159.1"
                     /translation="MSYQVRYEREAQKTLKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5326..5865
                     /locus_tag="IVO76_000164"
     CDS             5326..5865
                     /locus_tag="IVO76_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428160.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            6356..6640
                     /locus_tag="IVO76_000165"
     CDS             6356..6640
                     /locus_tag="IVO76_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428161.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6647..6892
                     /locus_tag="IVO76_000166"
     CDS             6647..6892
                     /locus_tag="IVO76_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428162.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6914..7168
                     /locus_tag="IVO76_000167"
     CDS             6914..7168
                     /locus_tag="IVO76_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428163.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALAYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7250..7435
                     /locus_tag="IVO76_000168"
     CDS             7250..7435
                     /locus_tag="IVO76_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP7428164.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7502..7897
                     /locus_tag="IVO76_000169"
     CDS             7502..7897
                     /locus_tag="IVO76_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7428165.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7887..8264
                     /locus_tag="IVO76_000170"
     CDS             7887..8264
                     /locus_tag="IVO76_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428166.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8265..8660
                     /locus_tag="IVO76_000171"
     CDS             8265..8660
                     /locus_tag="IVO76_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428167.1"
                     /translation="MKQEKRKMKQTQTEPINDTLADQLAGYYKLFEMSKAQRLIHYLW
                     KYKILFLFWEATLTFSVLFYKSDCYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8966..9364)
                     /locus_tag="IVO76_000172"
     CDS             complement(8966..9364)
                     /locus_tag="IVO76_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7428168.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9560..10321)
                     /locus_tag="IVO76_000173"
     CDS             complement(9560..10321)
                     /locus_tag="IVO76_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7428169.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPNKLKKT
                     GEIVLPVKAIIKYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKN"
     gene            complement(10357..11580)
                     /locus_tag="IVO76_000174"
     CDS             complement(10357..11580)
                     /locus_tag="IVO76_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7428170.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKMFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTIKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIAELRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TDIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKFMSRLQNKVYPLYDRLSKDRGFESVVSHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            12522..13325
                     /locus_tag="IVO76_000175"
     CDS             12522..13325
                     /locus_tag="IVO76_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7428171.1"
                     /translation="MSQEVTENVIDNDVDLVSDQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNVFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQQETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFESFDYEKIYAKKGRKVDRLRFTFSEPESNLPSISMHNWLEED"
     gene            13932..14375
                     /locus_tag="IVO76_000176"
     CDS             13932..14375
                     /locus_tag="IVO76_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428172.1"
                     /translation="MDAINWHEFVNYMEKLLDDLKSFSIADAVKHGLPVSTTMKMRGL
                     KVPDEIIKLRTKTIIETAKIVDSMNLDLKGKKTTYEKDFVRFLKNVIGKYLGKKSFTE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIVEYLENKEQKKGR"
     gene            14494..15552
                     /locus_tag="IVO76_000177"
     CDS             14494..15552
                     /locus_tag="IVO76_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP7428173.1"
                     /translation="MEKDVNSASTKDQYNIIGGLLGDKDKRTRHGLRIMLATFLGVFL
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLTFARSGAEITISDVWTDKTRDVTVVKLG
                     YSGNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDYTADDDTKVNVNESDSVTKALSQYSMNDVDNEGVISFGKKAKPVN
                     TPDNINFRINPYSDTTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKTINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15594..17543
                     /locus_tag="IVO76_000178"
     CDS             15594..17543
                     /locus_tag="IVO76_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7428174.1"
                     /translation="MENSTEFFKNIQTEFNHALTLMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAKRQRQEGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TIIPRLKEYERILKSEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPEASLK
                     LSKKIPDELLEEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDIAKLEEELKSIKGQPVAVYTVTMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRKLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVNDTVSLSNTILFATTNAGAE
                     MYDSMNRYSSLSRVANQENSNEYSTELKREYRKVALEIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAHNEILKSAELVRKHLNVDLRLPPQKKHFYGQEFYADDLSMHLV
                     FDRASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSKAGGFEGKGDT
                     DAEIIAKPSIAESKENKFVFSVGGV"
     gene            17546..21235
                     /locus_tag="IVO76_000179"
     CDS             17546..21235
                     /locus_tag="IVO76_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428175.1"
                     /translation="MKFKKSLLQNRKIKRWVVRGLVIVGALAVTLGAPFGMNDPTSDD
                     QGNKQETVANTNVAYAESKPAEKGVWTLYARILMGDKEVNDSATKTGQMNLLGQGGIS
                     ADFPYSDISKQGNAIAGGNAGTQLASTLATYNYYGYIETVSGNAIASQASSFIGGIGR
                     TLGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGKGGSDVSNPVAKALTSLL
                     NNLGLNNQLFKAIASLSMLFFTAILGYQGMKLLSKEGVNSKGLSPVLEKWIVRIFVLF
                     VFLPVAGSLLGVYINGIQSAKEDGKLPTNVVGDYIINTRLWASTQNLAPSSDTGGNVP
                     NGKPTSEIKFVDTDYEPSKKSEMIRMINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NVNTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGNAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTTNNFDATQPWGVANNATFSTQSVALMLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFVRVWEVVLTGSLAAGAGVMAISLGVVTTSLIGLQLPIFLITFVRAFGDTAV
                     GFIPNTLLGDGVANIVRSAMVLGVGYLLVFKKGEKVNNNLINMLIGLPLEVAFGFDER
                     AKLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGRGSVKDMVAGGLGAVGAQGIAD
                     RLNNMGDSNADAKTIADKVVPETPASVPFNGGGVSGTINNTSFKDLVRQDQLRNNSQQ
                     YSDHLGRMVDEKGNFVDENGEVLKDSKFGKDAKGNFIDDKGRLVDEDGNLIDTNGKPL
                     EDNEDGNIGMDSQGRLIDDKGRLVDDEGNLVDENGRKLLNGDFGQDVNGRLVDDKGRL
                     VDEDGNLVDEFGKKLGKHSNGYNVNGDTVDEDGNIIDSRDALVADRNKELEQQQHTPI
                     AVAIPDDESDEVILDQAHQGLREEAKDKVVSDQAQEELKNKSNGEDVSNQYHQTAGNV
                     PVEGQSNELHNNFDNVADRNDTVLKEKESLRQETDKDQSKLKKTTIPTPIGSTVPKVN
                     QANDSETNTGLGMAKDNQSIREPKKTTESSKSNKSKSTSNKTGKSKEPSKAKSAVEYL
                     SKTGKNVYTSADKALAKAPKGSLAGMTYEAKNEAIKLGKGTAKVAKVTAKGATKTGKA
                     VKKSTMSVKDYIAKYSEQKPTDKKS"
     gene            21647..21985
                     /locus_tag="IVO76_000180"
     CDS             21647..21985
                     /locus_tag="IVO76_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428176.1"
                     /translation="MFDSIKNLVSGMTPEMKTLVQAILYLMATFFALKPGFTAMKHFG
                     DAKWGEGLKYVGAVVAIFIIPVITTVMLLTMGTKTGNDLNQKANMISALIPVVGIYLV
                     TKFPKEEQLG"
     gene            22097..22333
                     /locus_tag="IVO76_000181"
     CDS             22097..22333
                     /locus_tag="IVO76_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428177.1"
                     /translation="MDREKMLVHANEVLTSGFKGTDLAKIMNMNVNQFYDYRNGNKKI
                     EKARLETLIKFEKAYLYVLDKQKRTIDRKKGVLQ"
     gene            22333..24498
                     /locus_tag="IVO76_000182"
     CDS             22333..24498
                     /locus_tag="IVO76_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428178.1"
                     /translation="MAKFKDITESINYVAETVPVVVLSLKPQLLEVYQKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYVVIEDVQKLSSEEFPDEEDAILDSEKYVVLLKSSLN
                     YVVDLPEKFKEKLLAGVRFMDSNGQLLVIPDDPNMFEREVEEATDFYSAVDQLEVATK
                     GLERIAETFNSSDAVEFEAVAEAETEAVPDEEEALFEDNDEATEAGNQEASIEETQTE
                     TYQQPKIDEKPESQSDKENYVYEAEKIVSESDVPKNLDADLLEKDFEQIIANKFQPVV
                     LDEVSDPVDLNEEMRSIAELKPLLFLAKESMNARISIANNKINSELNKTKEQLLLKGK
                     QMLADELLRIEEATSLSGQNQFTNTLSVAKESYEAEASKIDDLVMEKSSIIRKDYDMA
                     KQAYLEAELKKLEQRYDAEHLPEVKQQEDEFRKATEESLDQQYQEAIESIKNDALKQN
                     QEAKEVVVERIINSLKPEIEKSLTEVQEKTLEIVEKTKAENEAEFHDTKEKTTDVVVK
                     YFANKEEVEKLVAKDTNELESKLVAQQNIIEDYKNRVKEFKDLADGYKLNSQKLTEQS
                     EALREDLSRARNDLSQMAEKTKSLVVTTENQTESKRDFTSKKMATIIAVSAMACGTIL
                     GSSYFISHNHQEAQAQTETSISRVENSLGSNTASTSTTDNNYEATPTTEFKVGEDVVI
                     SIDGKTTKAEVLSIDGDKAVVKAPDGNQYKIQAHLTEKN"
     gene            24510..24740
                     /locus_tag="IVO76_000183"
     CDS             24510..24740
                     /locus_tag="IVO76_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7428179.1"
                     /translation="MREENIVYPVIENQANTKSSQFLQEVQVVYPVNELPQTNETVSK
                     SISLIGLLIVATALTVGVYRKLKVKGSISDGR"
     gene            24730..25596
                     /locus_tag="IVO76_000184"
     CDS             24730..25596
                     /locus_tag="IVO76_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428180.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVDWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLRFIFIPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNTARVTMGKKKPFRGTEILKVRQDGTIMYRDGSVALMFNLDGATSLTAFP
                     EEIIAQEGKSIQYQNARDRSLAEIKITSSQPQNTERQIRNMKVQKRNAKSNKAVFDIV
                     DQQERFVSEYIDGVKTTVIQYLILKSQSQMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSEIYALK"
     gene            25614..27572
                     /locus_tag="IVO76_000185"
     CDS             25614..27572
                     /locus_tag="IVO76_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428181.1"
                     /translation="MELMSRSKLLNFRKKKHKKEQNADSSKAVNNTYVSDLTSLVGYQ
                     FTPSYIRTGNRWGTIVKLVNTYGMNRNYEFGWMVKAIPEVKVEGVKAYLIEADKPVSQ
                     KDQQEIMHENVGGSTNSYENNLNDKNSDAEINKTVMRIIDLNTASLLDSTANKIIDWK
                     LEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSFGGDQRDLFDRLLSEPVGSVYEY
                     TSMSSDFAGNDHAIRKGLDDADGWAIGTMSTSYASGVAFMAMDRSFKGKVLVASHKDA
                     TIKGYREEISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGETFVAKPSLNSV
                     IEKINLAEEGLNSLELFGDRKDIGDLYRRNLDKLTQIFYLLADRELNPATRVLLKKEL
                     GNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKTGTEKEIDRII
                     VLKDTLRTALDSFSKMFNQRTTLKEIGKSDKLQFYYNLSDLRTEINVMEAQFLNTFDF
                     ITSQAKKGDIVMIHGFDRLSVETVGLIKKRLTMLEEDGVRLAFLFDTIGSGEDKDGIE
                     TADIFNTDGKIYQSFESDFNYTIIGKMTSEELMKYEKKVAPARKGEDDQKLSVDLRQY
                     LTSGRSNKFQIRRPVDLTNNFVIGEFII"
     gene            27711..28046
                     /locus_tag="IVO76_000186"
     CDS             27711..28046
                     /locus_tag="IVO76_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428182.1"
                     /translation="MKQYLNEWKVIEGSLVAQRIKGLPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDEKYFIIQEYEGQLTPFYTTWEMSEDGINNFPCESI
                     EESISQTEC"
     gene            complement(28572..30167)
                     /locus_tag="IVO76_000187"
     CDS             complement(28572..30167)
                     /locus_tag="IVO76_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428183.1"
                     /translation="MFILDDEINKVKNPRIVPLLEEVRSSFYQGNYRSAIAVNYSALV
                     LDLIDKLSDLANIYEDDTSKKILEEIAKLRKSDPNSPRWEMELLEKTYNSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGDTWELREITKETCQDVIRKSYEIVFLKNPILGKKV
                     TNEIIQYASRIHSMSVTPEEFHSALKNSYLKKLSEKAKENLCKTAFKFVFKLPYGNEE
                     VDRDRVSTFKLLDALIFDNKEFYLKILKKEISNYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIASHPELQRDFSEEIIQNLKISIKDFEPKQPITTHIEDYYRLRSLAVLGNT
                     KEELQEHLNRLLDPILQQNMRVSSLATSTLETIYNQYLYFGEKEMFLDFMIEHLTDSP
                     AFRYSDKDMEILPWIYDKLNQDQFNKLLYNLNRNNQFYYNGYFSSFISNLLDFYKQKF
                     HMDLRTHYNGLLYPNAVTMDQKFQLKDKELKKLYELAESKSDTYPEILNDLKLNLVNK
                     SEQYLGEKINSEEKLLDYIKQIK"
     gene            30288..30872
                     /locus_tag="IVO76_000188"
     CDS             30288..30872
                     /locus_tag="IVO76_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428184.1"
                     /translation="MGLKQSVYIWLYPDNPMSIMADIFVGSILLCLLYLIFFHNLIGY
                     MILDWKGMFDIERKPFNNKQIYTTFAGGDLEEKEVDVTHEIGLALSGIQDADVLGSMI
                     TDTGYNAYEVFYLMYFIWRDSQDIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     NTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            30883..31134
                     /locus_tag="IVO76_000189"
     CDS             30883..31134
                     /locus_tag="IVO76_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428185.1"
                     /translation="MKKIIDQPTNRLIVEGVIFVTMYFTVGNGISNTIIQKDPQYLLF
                     SLYAWIVQMLWVSLLMLLFYWIVRVILLVLNKAKKKKDR"
     gene            31141..31863
                     /locus_tag="IVO76_000190"
     CDS             31141..31863
                     /locus_tag="IVO76_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428186.1"
                     /translation="MKATKLKLVLFSTAVLLTLSACGTSKSADNTKDTKSSSTVVTES
                     SSAQTSTTMSSMTENNAEKFQKYKDVNQGKKPEEVYNEFISSVKNDESYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIQEGFSYAPTLMHDITIGKPAYEYNAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTLAVVTPKKIDGTLYWDLMGMTTNTSDEMIESTSNYYM
                     DLGAYKSYEQSEPVMDDQSTIK"
     gene            31993..32736
                     /locus_tag="IVO76_000191"
     CDS             31993..32736
                     /locus_tag="IVO76_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428187.1"
                     /translation="MKEISASEAHISQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQELVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            32742..33107
                     /locus_tag="IVO76_000192"
     CDS             32742..33107
                     /locus_tag="IVO76_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428188.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKQRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            33123..33653
                     /locus_tag="IVO76_000193"
     CDS             33123..33653
                     /locus_tag="IVO76_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428189.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            33815..34180
                     /locus_tag="IVO76_000194"
     CDS             33815..34180
                     /locus_tag="IVO76_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428190.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     RKLAQSYLEEKAGVIKDKE"
     gene            34197..34586
                     /locus_tag="IVO76_000195"
     CDS             34197..34586
                     /locus_tag="IVO76_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428191.1"
                     /translation="MNEETELFLKKVNFLVEKYFLTKHKIMYLIKGLIGGIILWILTF
                     FSLFGGFTNYKKLVDENVKQISASESLRHQAEDLVSNPLFIGGMVEVIIISFVIGYLG
                     MCIFQIIFDLIRGKVFQEFKSEKINLR"
     gene            34616..34840
                     /locus_tag="IVO76_000196"
     CDS             34616..34840
                     /locus_tag="IVO76_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428192.1"
                     /translation="MEQTNHITEETRKFICLESFYSEGRYCNKGETYTAYPIEGGFKL
                     VFENGDMNFTTELFECVLETWSDVLLEVTK"
     gene            34893..35174
                     /locus_tag="IVO76_000197"
     CDS             34893..35174
                     /locus_tag="IVO76_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428193.1"
                     /translation="MAYENLQLVVAGVSGDIYLAKAKDGIMDTNYRRVATNDVLVATT
                     EWFIRNNKKLIQFEADENGRHSLFYTNDQKKADRIIAILKENDDGGQRD"
     gene            35155..35397
                     /locus_tag="IVO76_000198"
     CDS             35155..35397
                     /locus_tag="IVO76_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428194.1"
                     /translation="MVDKEIKGEIRLFQRENDDWGTEISKNLAEDIELRLSFISMIIE
                     SFAFELGIPQEKLAEILIKASNNSPQHFHIDLGTVN"
     gene            35519..35767
                     /locus_tag="IVO76_000199"
     CDS             35519..35767
                     /locus_tag="IVO76_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428195.1"
                     /translation="MSENHLKRLSKGTASMFDANLLIFKSLLEFEKQRVEDIAKYGNY
                     ATVPSVYNDLMKWFKVQTGLEYSQQNLVVKIEEYENHG"
     gene            36042..36254
                     /locus_tag="IVO76_000200"
     CDS             36042..36254
                     /locus_tag="IVO76_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428196.1"
                     /translation="MPVKTNTEWLDICDELFDTKDKKELAMRVAYAIVQKDKYFAVPS
                     TDYDIEVIVDPITGDKKLRVVIECNA"
     gene            36363..36539
                     /locus_tag="IVO76_000201"
     CDS             36363..36539
                     /locus_tag="IVO76_000201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428197.1"
                     /translation="MDIFEVKPDSGKFIVLQEKRVIAIERTEIEAYKKIHELAEQYIE
                     DLNNNFQEFLTQNE"
     gene            36650..36871
                     /locus_tag="IVO76_000202"
     CDS             36650..36871
                     /locus_tag="IVO76_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428198.1"
                     /translation="MDIYEEVICDVEGKEEFLPCIKELETTVAITKVANAVSNETNEL
                     VGIHEAGNLVFSCLNSNKSALLALDGDLF"
     gene            37243..39483
                     /locus_tag="IVO76_000203"
     CDS             37243..39483
                     /locus_tag="IVO76_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428199.1"
                     /translation="MSEFEFLDKSIGSAFLIDPDLFGAWAQSSGYNWAFSDDMVELLS
                     QTMNWEAYKIKQFLDVVAQPIYTSADDHWSGRELTTLKELAEYNKRYKIPFQVSYMSD
                     DFSVIYSIGKESRYLIPNRHLEIKMNSDYTDYTLSELKALRQSDSEEFSALKNEVSAD
                     NFRRSDIEELKKKQKQQLEKQKKLIEDIEYKRVEELAIQEAKIEKMRQELYKKQQSML
                     AEANKMRFELEKKVEDLNTKIFYLETEINAIEGYFGESTTFVQLRSGQKSLPEEPVVM
                     FQKVRYLDEELGRYFSMREVEGTADTMLTFEQALSSRDDLFDLFSPNKKSISMIRISK
                     SGYGYTNGQMESTNMLEKYKKYHGETLAILLRNGDNLWICWLDENKISFKDDLFYQNK
                     VTEQASETKNMVKEIDWTHTRNLEERLKDAKKQKKAVRGEFASRYILFSILQGVLDNG
                     TMMTLPEKVNVIQAINQKSPYFIASVADGWLEDTRLPSFSELIDKYSFYKETGEDRVL
                     EAEYELNPDKFENMRDYPHKQNDDIYVLYRLRGWSPTSANYTYYNSDNRSNSESGRDL
                     AYSIDLKDGIYQINLVKQGKFQKQMFSRTFVGVDSTTGENVYDYYYERETEYYIRGQK
                     AYNKSDYWGDKAKRLTANFRVYESEFINLTFLNSVFIDYVITTKRIGSYSSTYASLVP
                     MLNSMSRFLKEREEKELEYIKEIMPDFEMHEWQIALSDWKLTNKVRRITPFQAKRFLK
                     AMKFVQ"
     gene            39621..40418
                     /locus_tag="IVO76_000204"
     CDS             39621..40418
                     /locus_tag="IVO76_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428200.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYGESQNKPRKKKQAYVLKMVNGE
                     ELIVLGYSKEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYNSNRFDRLEKFCRPYTVLKTKKFNKTKRVS
                     IKLPTGQISLGEKDYLIKVGKELIIKVPERLFKENFEIRETLVWKEE"
     gene            40458..40640
                     /locus_tag="IVO76_000205"
     CDS             40458..40640
                     /locus_tag="IVO76_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428201.1"
                     /translation="MAVNRKLQVLITDDFGGAEWFDVQSIDFEEEVVVIENVYGEEYS
                     YSLEGENVEDLRLIGE"
     gene            40684..40971
                     /locus_tag="IVO76_000206"
     CDS             40684..40971
                     /locus_tag="IVO76_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428202.1"
                     /translation="MINKKTGKRIFSSKDEVIRDLSENFEPIELFFHQLNLEIEKLLK
                     EPTEGRINKVLKLIACKDSHAYMLYPKWYADKNIAEINDYLASIKERNAIN"
     gene            41008..41295
                     /locus_tag="IVO76_000207"
     CDS             41008..41295
                     /locus_tag="IVO76_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428203.1"
                     /translation="MAEVKFGSKEWFEELNSMIQFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            41386..41625
                     /locus_tag="IVO76_000208"
     CDS             41386..41625
                     /locus_tag="IVO76_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428204.1"
                     /translation="MKELEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHFPKVRFYN"
     gene            41753..42082
                     /locus_tag="IVO76_000209"
     CDS             41753..42082
                     /locus_tag="IVO76_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428205.1"
                     /translation="MKLEGSLQNRMMEGRTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELIKTKNGWKVLKEDTYFYIGDRE
                     KYYDPSL"
     gene            42135..42983
                     /locus_tag="IVO76_000210"
     CDS             42135..42983
                     /locus_tag="IVO76_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428206.1"
                     /translation="MFSQEEIISKSQPDKNGTITMKKKIPCYRCSGSGQYKFMSMGKI
                     TYGTCFKCDGDGFLVVTEKSFTPEYRAKLNALNKKRADRKIRITREKESQKNFDRYGF
                     EKGYIYVVIEENSYAIKDELHHQGATYDPLLGWFFKEKQEQYKTQKVFVEEFSVINDE
                     GIIELKLEEYSERQQEILRAKRREEGEFIGTIAQRLELDLKLTNTVTIDTQWGNVYLH
                     LFEDQNHNSLIWKTNKFLYYDEWDVGEIRKVKATIKAHDYYRDTKQTWVKNVKIQKNE
                     SEGIKV"
     gene            42980..43297
                     /locus_tag="IVO76_000211"
     CDS             42980..43297
                     /locus_tag="IVO76_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428207.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            43300..43533
                     /locus_tag="IVO76_000212"
     CDS             43300..43533
                     /locus_tag="IVO76_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428208.1"
                     /translation="MLSDLFILLIYLGVSEIIAYFLFVIANYGISSAIFQRFRLPVSE
                     LKKMMRLSAYLLMFLNVVTLIFVVIGVTFNLME"
     gene            complement(43650..44129)
                     /locus_tag="IVO76_000213"
     CDS             complement(43650..44129)
                     /locus_tag="IVO76_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428209.1"
                     /translation="MTDKTSDLPKLAPKTSRVKRKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            44249..45586
                     /locus_tag="IVO76_000214"
     CDS             44249..45586
                     /locus_tag="IVO76_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7428210.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVSGDVAKNIDDIYKFMNGEYGLSGATIAGILGNWKQESGNDPRTVEG
                     YYGDGQREVEFIKQFTDGLMGIGYGQWTAGRHTALVNWAKKNFGGAWWTTEAQLDFMF
                     KGDGGFVAILKDYALNASDDVVANAVAFHKNWEISADSESAVVAVRGGNAKEIWEYMK
                     SKGMDGAKDVSKINKIAGGTSTGGGSNNSSSSASGTVEDACATTDDANSGSGSMGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            45609..46136
                     /locus_tag="IVO76_000215"
     CDS             45609..46136
                     /locus_tag="IVO76_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428211.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSDKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            46123..46686
                     /locus_tag="IVO76_000216"
     CDS             46123..46686
                     /locus_tag="IVO76_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428212.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLENLDSYVRAE"
     gene            47109..47846
                     /locus_tag="IVO76_000217"
     CDS             47109..47846
                     /locus_tag="IVO76_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428213.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(47863..48279)
                     /locus_tag="IVO76_000218"
     CDS             complement(47863..48279)
                     /locus_tag="IVO76_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428214.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            48671..49918
                     /locus_tag="IVO76_000219"
     CDS             48671..49918
                     /locus_tag="IVO76_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAP7428215.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            50188..>51626
                     /locus_tag="IVO76_000220"
     CDS             50188..>51626
                     /locus_tag="IVO76_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP7428216.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDG"
BASE COUNT        18690 a         7519 c         9812 g        15605 t
ORIGIN      
        1 acttttgttt tgtgttaagt ttctttacat tcctacgccc tttgtgtgta aaatttcttt
       61 attcctccca cgctccgtgt tattttatat ttcctttttc atgttatttt tcgtcagaaa
      121 ccgatctagt ttttacctat tcccaaaatc catttattcg ttatttttta acctgattaa
      181 ggataattat ttgatgctta aattagctaa aaaggcgatt agttaccgat ataatcaagg
      241 tattaaagct gaattaggcg gaaaggcaaa atgttcgcaa aatcaaagta tcaattatat
      301 tgttttttta taaatttgta agtgaaaatc tgaaaaatcc tctttaaatt ggtataggca
      361 ttaaaattac atacaaccac tcttaaaagt atgcgctttc atcgttttat atgatattaa
      421 tatcaataaa taacgatata aaaaccattt atcataatgt tttttaagga tataaattga
      481 tctaatttaa aaaaataatt caaaatttaa aagttttaat attacaagta aaattatgtt
      541 atcgtaaaat ggtataaaat agcttaatat tctatttttg ttattttatt agcaggaaaa
      601 tctaaatatt tttacaagaa atcgtagaaa tatattggtt tcgccttcag attaatactg
      661 gatcatatta atctgaatac aatatatgaa ctaataaata gcttaacctc gctattttgt
      721 tttttctgat cattatatac acagcattaa caaatagaat tttaaaaata aaaaaagtaa
      781 gacaaaaaaa gtatcaacat aaacatatac atatacacgt ttatgttgat acacttaatt
      841 tgtcctttaa aaagcactga tttttaaatt gcgttatttt ataacccgaa tacagtctgc
      901 ttgttaggca ttgtccttaa attacatttt tcataggtta tttaatcatt taatctgttg
      961 attattcttg atctaaaact tagcttaaca aatatataat cagccaaccg cctgataaaa
     1021 atgcgatcat tgctaatata taagcaaaga taaaaagatg ttcgatcagc ttaaaccctg
     1081 atgcgattaa gcagaccaag taaaatagaa attctcttac tgataatggt aattttctaa
     1141 ctagcatact cataattaac accccaaagt aatattgtat tttgaacatt ttacatccag
     1201 tacagcatca atcaaccatt gccaatcaaa aatacaaggc ttcattgaag tatgtttttc
     1261 agatgaaaag atataatata attcctttgg aataatattt tcagttgttg ggatcaagct
     1321 aaatctaatt ccatctaact catagctacc agctttctct ccattaaaaa tttccttagc
     1381 actttcttct aaagaatcaa aatatttttc gacagcaacc tttgaagctt ttactattga
     1441 agcatttccc ttttcgataa aatctgcttt tgttttaccg acaataagta attcttgatc
     1501 agtaaaatct tctaaaataa tggtagtgta attcttattt cttctcaatc gcatcaggat
     1561 cactccttaa tataatttat gtttttatga tatcataata agataaattt gcaagtattt
     1621 gtttacacct ttttatttac actttattat aattaattat acttttatga ttgcattttt
     1681 ttcttataaa cagttgcaac taatagtaaa taggtgtaaa ctattttcgt accgaaatac
     1741 cataaacaca aacagaaaga ggtcatacca tgaaagaacc attattgttt tccgttgact
     1801 tagggaataa atcagtaaaa gaaatgagta cacacgtttc tccactctca cttccctctc
     1861 gtattttgaa caaaaatatc attaattcag gacgtttttc taatataggt tcctcattaa
     1921 aacgatcact aaacgtttct gaatatagcc tagtaaacac tactgaaagc tattattttg
     1981 ggaaggatat acacgagcta ggaaaagatg actacgtgat agaatcacta ggttttggga
     2041 ttagcagata caagcaggaa acctaccaaa atatcctatc tttctcttta gctaacctag
     2101 cacgtttaga agatcaatct gatgacgttt taaccgtcaa tctagtttta ggtctgcctt
     2161 catccgacta taccgaaaca atcgtcaaat acgtgatgca acaagccaca cgacaacatt
     2221 cagttgaagt tgacggtaag gcttataata taaacgtaga taccgtttgg gtactgccac
     2281 agccaatcgg aacgctatat aacctcctac ttgctgatga tggtagcgta caagatgaaa
     2341 ccttgatcag tcagaagatc ggttttgttg atattggtgg tggcacgaaa ctttttgatg
     2401 ttgtccaaaa ttttgaaatt gacgaaaaca atcgtgaaca aaaggatacc ggcattttta
     2461 ctttatataa tcgtatcgta aatatgcttg accttccgat cagtcctaat gctttccaac
     2521 ttgaaaagat tatccgagaa ggcaaagaaa aaggcgtata tctatttaaa ccaagcggaa
     2581 atgaaagttt tgatatttca gaagtaattg aaagagaaat aaaaacatac acaaatatgg
     2641 ttgttgcaga agttttagct acattcaaaa acttcacgac cttagataaa gttgtcttta
     2701 ctggtggtgg agcttctatt atcgacaaac aacttgtaaa agataaaatt ccgcatacct
     2761 tgtttgttga agacagtgaa ttttctaatg taaaaggctt ttacaagttt ggtttaactc
     2821 aataaaacag cgaaggagat caacatgaat aagggaaaat cagagcgtta tgtagttata
     2881 atcaatagag aaaaggttag cgagttacct ttagcagagt ttcttgaatc cgaatatgaa
     2941 acactaggcg ttaaatactc tcagacgatc aaaaaagctt tgaacctata tcggcgtgta
     3001 aagaacaatg aagttacaat tattgaagca ggcaacgata gaaacgatag caaagtagca
     3061 gaagtaaaga ataaaccaac tgatcataac cctattcaac aagagcatcc ttcaacggtt
     3121 acatccgatc attcagcaaa gaaagaaaag ccaatgctaa aagggttcaa tgcaccaagt
     3181 aacaaagact tactttaaaa tgaaaaaggc gaggttacaa tacctagcct ttttattatg
     3241 tacaaatata aacataaaca tatacgtata catgtatata aacatgtata cgtatatatc
     3301 cataaacacg tatatgaaat ttagttaagc tatacctaac cgcttattcc aataaaaaaa
     3361 gtgatcagct ttaaactgat cactcaccaa aaacttcatc atacaagtta tctatcattt
     3421 caccaagtaa agctgaatct gatcgaattc ctaacttttc gctgatttta gatagcttct
     3481 ttctttgcga tggttgaagc gtgaattgat aatttttctt cttttcagaa ctatcacttt
     3541 ttagtttagc taccaaagaa cttgttgttt ctttaggtgt ttgtttaggt tcttcaactg
     3601 cttcaaccac ttcaggaact tcttctactt cagcttcagc gaatttttta aatctatttt
     3661 ctttcacttc aatcactcct tattcttgtg ccgtatcaat tatcttgttg tatagcgtaa
     3721 acgtgctttc aaagaaatcc ttattcttac gatagatatt cttatcctta gcgaactcag
     3781 caatattctt accttctaag ctagttgcat taagcaattc cttctcttgt acatatccaa
     3841 gataattaga taacgttggt accaattcta ggaactcacg acttgaatta gtattatgtt
     3901 tgatcctgct accgatcgta gcgtatttag tttccatcag tgtttcacca gtcttaatat
     3961 caatcaactg ttttgctagt gcatcaatat ctttaccaag cttcagcaaa gcttcaaaac
     4021 catctttaga tggatcagaa atgcctacga caagatcaga tacagcccaa gcattttgag
     4081 ctagtagacc tgcatcatta tgagtatcaa tcagaattag atcatacttc tctttaagct
     4141 gttcatagtt aacaacgatc catctaaaca gtagggttct gttgttaggc atatcacgca
     4201 tttgcatctc aacttgtttt aaatcttcac ctgctactaa aatatccatg ttctcattta
     4261 tagctaaagg ttcagctgat ccatgaagat ataaatgatt gattgtattc tctcttttcg
     4321 ctttactgaa agcatcagga aagcgttttg ataggttttg cgacttatct tcatcaatat
     4381 acagcacctt catacctaaa ttttcgacag catatccacc tagattgtag attgttgttg
     4441 ttttacctgt tccacctttt tcaatgtttc cagtaatgat tgacaacttc ttttccatga
     4501 ttaaacctcc cttattaacc ttttgtaact ccattataca ctataaacac gtttatgtat
     4561 atgtttatat acataaacgt gtttatattt atatttgctt ttatattcta taacataaac
     4621 atatatatgt atatgtttat gtttatgtat atgttcatat atataactat tgcatctgtc
     4681 atacaatcgt agtacaatgt aattgaaaag gagttgataa actatgccag taataagtat
     4741 tcgtgtatct aaagaagaaa aagattttct tgaaaaagtt gctgaattca acggagataa
     4801 catttctgaa tttatgcgta gtaattccct gcaaacagca gaaacgttag tagactttgc
     4861 cacctacaaa gaactaatga aagagcatga ggaaaacgat caaagtattt cccacgaaga
     4921 tatgctaaaa gagttaggct tataagatga gttaccaagt aagatatgaa cgtgaagctc
     4981 aaaaaacgct aaaaaaaatg gataagtttc aggcaaaggt tattctaaac tggatcgaga
     5041 aaaatcttgt aggcactgat gatccaagaa agcatggtaa aggtctaaca gctaataaat
     5101 caggctactg gcgatatagg gtaggtgcat accgtataat agctgatatt agcgagcatg
     5161 aagtgacaat tcttatattg tctattggtc acagaagtga agtttataag taacagacaa
     5221 gctgataagg cttgtctgtt tttttgttaa cccaacaaaa tcactgttta cgatcccact
     5281 ttacctaaaa aagaacaata tcctcttgct attatacaca ccagtgtgta taatataagt
     5341 gtaaacaagc taattaactt tcaaaaggag cgatttttta tgggatggta tggtgcttat
     5401 tcatcagcaa atgaagtaat cgaagaaaag aaagggtacg ccaaaaatgg tttggaaatc
     5461 gtagatacca aagtaacgaa gacctatgga attatccttt tcaaagatgc agaaggaacc
     5521 tacgatattg acagcttcat ctttcgagat gaaatgtaca aaccaatcta cttcttaaat
     5581 gacagtcctg cttactttaa gaaaattcca aaaaaatggt tgaaattctt tgtcggtgat
     5641 caagaaaaaa tggttaagcg acaactagat agaattaaag aagaaagcaa taaaccaaaa
     5701 cttgatgact tattagttgc aggtaaaact tactcaatct gggatggatc gtatgtcgct
     5761 gtttatcagt ataaactcaa acgtagcttt atttttcgac tacaagatgg atcactcact
     5821 cggttcactc gactatcagc aggagatgtt aaggaagtag cctaaaactt aaatacataa
     5881 acataaacgt gtatacgttt atgtttatgt atatgtatat gaatatcaac gtattaggta
     5941 cgaacgcttt taaatgcttt tctgggcttt gattttaggg tagggctaaa acatcaccat
     6001 ctgatccaaa acagctcaga aacgcattta aacgtatctg ataccaatat tacagaagga
     6061 gtgtatcgta tgattaacct atttctttta gtgacagcta gtacattatt ctttactgga
     6121 atatcactaa taatttccta tttgatctat aagaaagccg aaacttacac aagcaaagca
     6181 aacggactag ccgtttcatc ctttttaact ttgctgactt tttttgttac tctatcgttt
     6241 tcttttgttt agcacagctg atccatcagc aaaaaacctc tctttacatt atacacattg
     6301 gtgtgtataa tgtaattata aacaagctaa ttaactttta ggaaggagtg acaatatgct
     6361 aacagctaca ttacagcaac ctactcacgt tgctattgca gaaagctata caattaatgc
     6421 aggggtcgtt aaacagctga tcaaggatcg agaattcaaa aaatttaaca gtcgtcaagc
     6481 gtataacaga cagttaaaga aagctattct aaagcacaaa aattccttca tcgggaaatt
     6541 ggaaagtgtg gatgttaaaa ctgtttcaac ggcattttca gttgttgaag tgactattga
     6601 cggtcgcttg atttctctat ttgcttttgg aattaattga tagggagtgt ggactttgga
     6661 aggtaagact tttgcagaaa tttggactga atttattgag gtaccaactc gcattgatcc
     6721 gataacttct gaaaaggtat tggatgcaga cttctacaat ctcaaagcag gtactagcct
     6781 tgaatgcgtt tggatgtatt tagaagagca tttcaaaggc gtaacgctcg gtcacgtctt
     6841 ataccacaat ggtgccgagt gggatgcaat tggaaatatc catttttctt aatacaagaa
     6901 aggagaacac aatatgaatt ataccgatga agaacaaatc actttggaat acgttttcac
     6961 aaatgaaaaa ggggaggaaa ttgaaaaagt cttgatcatt tcatcaaaag acttggagaa
     7021 cgctttggct tatgaaccat acgaatcaac cgtaactgtc aatgaattct tagataccta
     7081 tacttctaat gatacttggg aaattatgaa tatttgcaac gctaataaca ttcaatttgc
     7141 tgaaaaaacc aataaatcat tttattagtt ttgcaataaa acatcctctt tttctattga
     7201 ttccatacac aacaatgtgt ataatagatt ttgtaaaggg aggggagcta tgcctaagac
     7261 agcaagagaa attgtaaaaa ttctcaaatc taacggcttc aaaaagactg gacaaactgg
     7321 cagtcatgca aaattttata attccaagac taaaagaacg gtcgttgttc cgatccacaa
     7381 tggtgatgta ccactaggaa cagaagctaa tatctggaag caagcagggc tttaacagcc
     7441 ttgccttcct atttggtatt aggcatacta tcctctctta cactagaaaa ggagcgagga
     7501 aatgaagaca aaaaatattt tttatccggc aatcttcgag aaagaaggag aagcttataa
     7561 catcgttttc cctgacttac cggaaatatc tacctttgga aatagtcttg aagaagcata
     7621 tttaaacgct aaggatgctt taggattagc gctatatagt gtaccagatg cagaatttcc
     7681 taaaccatca gcagtggaag aattaacttt gaaagccaat caagtggtag taatcatcca
     7741 actaaacatt aaacttttcc gaagaagctt aaatacaaaa acaattagaa aaaatgttag
     7801 tgttccggaa tggttagttc ttttagggaa ggagaaaaac ataaacttct ctcaactact
     7861 tcaaaaagca ctagaagaag agttgcttga aaagtaaaaa ggagaccgaa cctatgaaag
     7921 ctacacactt aaactatttc tctcgaacgt taatcaataa tctattaaaa gatacaactg
     7981 gcattagcgt tagtaccaaa cactttgcca atgatctaat tatttttgag gataataact
     8041 tgcgaattga gatgtttatt gaacaggcaa cccctaactc aatacctgaa ttctgctata
     8101 cgcacactga aaaaatgaaa catgcaaaat cagttaaaga tgttgaagca ttaagtgaat
     8161 cactaacgaa cgctttaaac ctcgttaaga aggcaaaatt gttatttgaa tttcaaaccg
     8221 actaccacaa tgagttgctt gctcaatctg aaattttaga ttaattgaaa caggagaaga
     8281 gaaagatgaa acaaacacaa acagaaccca ttaacgatac gcttgctgat caattagcag
     8341 gatattataa attatttgag atgagtaaag cacagcgctt gatccactat ctttggaaat
     8401 acaaaatttt gtttcttttt tgggaagcaa cactaacatt ctcagttctt ttttataagt
     8461 cagattgtta taatcaacac ttagttttag ggagtttttt gcttatctac accattttga
     8521 tgcttaattt atatacctac atcacacttg aagaacactt agtaattagc gatgcaggga
     8581 tcgagagaaa catcaaaagg gtatctttca agatcacgtt aaacaagcct accgataaaa
     8641 agctctcaga agcgttctga gagcttttga cattttcttg tataatttat ccaaacacat
     8701 tacaaagcac ctaattcgag cttatatcaa gcaaatttgc tatttattcc tcaatacata
     8761 tctaaaaatt acttgatgga aaactagata catggtaagc atgaaaagaa aatcaatttg
     8821 agcagatgca gtataataca tagcaattta aaagctctca gaacgcttct gagagctttt
     8881 aatatattct ttgataattt gtctttctta aatccatacg tcctaaaaca agctgttttt
     8941 gcttaatatg atcatctgag agacatcaaa tcaacttttc tctcaacaaa tcattcaaag
     9001 ctttcgacag attaatattc tcttcagccg ctaaaacatt taaatagtgt tccacagtca
     9061 ctgattttcg atcgtaattt tgcttgtttc gagctttagc aactttcgta ttagctttta
     9121 cgacaaataa attaacattc tcagatcgct tgattcttgc atttaactct tgaacaggcg
     9181 taggcgatgg aatttctttt tcttgcaact ctaaaagcac caagtatcct tccaatactt
     9241 cttgcgccat ctcaaaagca tcttctaaag tttcaccatg cgtatataag tctggaaaat
     9301 ctgggaattt aactgcatat tcatttcctt tccattcgaa tactgcacaa aaaaagtatt
     9361 tcatcgcatt gcacctctct aattaaaaaa taatttttgc tttttagttt caaaaaaaga
     9421 atcgcctacc aaaaaaattg ataggcgatc acaagctaat taacctctaa aggagattaa
     9481 taccttgctc ccaaggcact acatatctta gcatacgtct gaagcatctt caaacgagta
     9541 agatatattt tgcattaacc taattttttt ctctataagg tttttcatca aactcactag
     9601 aaatattaaa ctggacagca aaattgtaat ttctgccttt tctaacttta gcaaaatcaa
     9661 tcttcagcat tgatttttca ttaatttcct caactgattc ccttaaaaca cgtctatcaa
     9721 aattcgatat tgttttgtac tcattttctg ttttcgtaat ctcacgaatt ttaactattt
     9781 caatcagggg attcttatat atttcagctt tctcaaaatc tcctttattt ttggcgataa
     9841 gatatttttt ataatacatc gccaaatatt tgtataacac aatgctatat tttttttgaa
     9901 agcttgctaa atcttgaaaa acaaaatatg gatatttctt tttgattgaa agaattaggg
     9961 gaattacttc ttgcgagaaa ttgaaagtta taatttgttc ttctaagtcc caatgaattt
    10021 gatcaatagc agatactgta gcataagtct tatattcttt atctaagttc gatatttcat
    10081 aaactgcttg tctctgaagg gtgttaacta tattttttag ttctgtttca agattttgat
    10141 caacatccat gtatttgatt attgctttaa caggtaaaac tatttcccct gttttcttta
    10201 atttgttagg agagatataa ctgaacagca attgataaag ttcatacgac tttaaatcca
    10261 ttttcatgtc aattgttgct aagaactcct cgttgtactt tgtcagttca tcttttttca
    10321 aaagcttttc cttccttcac gcaagttttt cagttgttaa tcataaaggt tcacataagg
    10381 taaataactc gtaattgatt tttcgagata tttaacagta ttttttattg tgtaatcctc
    10441 cttatgagta gcaacgtaag atagatgact gacaactgat tcaaatcctc tatctttgga
    10501 taaacgatca taaagcggat aaactttatt ctgaagccga ctcataaatt tcagatcgca
    10561 taagtcggca gaactgatca aaaagttttt gattagtaat ttagtgtatt tgctattcat
    10621 agctttcaag tacgattgat tcattttctc tgttttatct tctgcacctt ttaaataagc
    10681 agggtctctt tgttcttgct tataaggtac cggtactgac atggtacgtg taagcaagaa
    10741 ctttatatct gtataagtat gacccttctt gataagctca tagtcaattt taaaatcagt
    10801 gtatttattc agttgggcaa tcggttcttt aattacacgt ctttcaaagt ctcgaaaagc
    10861 tttatatttt ttctcaatac tgaatttctt tcgtaactct gcaattgaaa aaacggggtt
    10921 ttttaatcta tcaagtgatt ttgtactcca tgttaagtta tctctatttg cttcatacag
    10981 attaaaatct ttagtgaata attgaaaaaa actgatcgca tatttgcttg tcaagttaac
    11041 tacttgatca aatgtatatt gtgtaaaatg attttctaag ttcgttaaca aaggcataat
    11101 tgaactacta aatttaatag atactgcatc agaaaaatca ttgaattcag taatttctag
    11161 cggtgagatg actttgtatt cagtcttacc gttaggttta gttctttgaa ttttaatgac
    11221 agattgctga tgtaattttt caataacttg ttttaatctt gaatgttttg aagaagatga
    11281 actagaagac atcaacgcaa ataaagcctt tttagatact ttgatggtat tattaaaagg
    11341 cggattttta gggtcaagat gtcctatagc gtaaagataa attttttgtg gcatagcatc
    11401 catttcaaca atgctatcca caagttgatt cgataaagta acagttaaat caaacatttt
    11461 tagattgctt tcggtaggtt caaatttcgg gtttacattc ttttctgtta ttttcatacg
    11521 attgcctctt ctttctaagc ttggtacgag cttaaaaact tggaactggt acgattccat
    11581 tgaataagta tagacctgtt ttcgatacga tacaataaga aatgtatcgt aaatttataa
    11641 cataactcaa tctcagtaaa acgcatttta tttttattaa tcaaatttca ttgataagac
    11701 agcagaaatc gctattttag aacctttaca aaataaaaat ccttttcaaa aaaaatacgt
    11761 aattataaaa attcacaaaa ttaaaaaaca aatatttttt tagtttttag aaataaattc
    11821 acatactttg tctttttttg tatatattcg gaataccaca tcgctttttg tccatgttcg
    11881 catcgcttta tgtccaccct aaaatctatt taagattgat ataaagcctt tttggagatt
    11941 gctaaagttt taaagattaa aatatatatt ataaatataa ataaaagggg aagctgaagc
    12001 ttcctccttt tccttttttt taattattca aaattacaca agcaaaataa actgctagat
    12061 agtgttagtt ttggttttaa ggtttttaat ttttgagatt ttgaaataag agaggaggaa
    12121 tcctccttta ttaaatatct gttaatatat ctgttaaata tctgttaata tatctgttag
    12181 gggggtctta accccttggg agagtaagta ttgaaggggt atgaaggtaa cacttttgga
    12241 cagtaaaggt aacaaaagtg gacagtaagg gtaacatttt tggacagtaa aggtaacaaa
    12301 agtggacatt tgaggtatca aatcaggata agacgttacc ttcggttttc ttgatttgaa
    12361 aatcgaaaaa gtagaaaact agattagtaa caaatctttg ttgactgtta cctttagttg
    12421 tgatttaatt taggtaacaa gcagaaaaaa agcgtttcct ttaatttagt accaaattaa
    12481 catgattaaa gggatgcaac aagaagattg gagagtaatc aatgtctcaa gaagttaccg
    12541 aaaacgttat tgataatgat gtagatttag tatcagatca aacaaatgca attgtgcgtt
    12601 ataacaatgg tttgaacaca acagctttgc gaggatttaa acctgtcgaa atggatgtgt
    12661 tttgggctat tttatcaaaa atgaaacgtc aaggaacagc agaagtaact tttgatttca
    12721 acgtctttcg ttatttggtc aaatacgaca gaagagaaaa ggacagtttt tattctgtgt
    12781 taaaagcttt aagtgacaaa ctaggtactt taacctaccg ctttgaagat tcagatgtct
    12841 atgaacagct gtggcttttt caaaaattcc gaatagataa acagcaagaa acagttacaa
    12901 tcaaggcaaa tgaggagttt gaatttatct taaattcaat tggcaaaaac tttactcgtt
    12961 ttgaacttga taactttaca aaactaagaa gtagctacac aaaagagctg tatcgtcatc
    13021 tcatgcaata tagaaataaa gagtggcgaa atggctattg ggtagtcaaa gttgaagatt
    13081 ttagaagagc gttaagtatt ccttcaagtt acagaatgac aaatattgac aaaagaatat
    13141 tagagcaggc gaaagaagaa tttttagctc ctgatgaaaa tggtatacag gtatttgaat
    13201 cttttgatta tgaaaaaatc tatgcgaaga aaggaagaaa agttgatcgg ctcagattca
    13261 cttttagtga accagaatca aacttgccat ccatttcaat gcataattgg ttagaagaag
    13321 actgaaaaga agcaggttgt aattttaata caacctgctt cttttgaaaa aagtatgggt
    13381 aacacttttg gacaataaat gcaagaagtt tttttgtgac caaactttat attagattcc
    13441 tgtgattttg tgtgttagaa aagccctgat agaaaagcaa attcaaacac tttttaatat
    13501 gtatgggtaa cacttttgga catgtcaata ataaatatat tttctttcgt cttttgccta
    13561 actaaaggta acacttttgg acagtatagt tttgtaaact aattagatcg ggtttaatga
    13621 gatatatctt agcatttatg taagaatgaa ggtaacatta atggacatgt caactgtttg
    13681 aacaaaaaaa tatagactat ttggatctga tttttcattt ttccaaacgt taaaaaatag
    13741 ttagttattt tattaataga tagtcatcct taatgtttga aatagcagaa aataagatat
    13801 tgtttgataa atgttgttgt tataaggaaa acttgaattt gttaattgat ttatttatgt
    13861 tattatgata ctataataac ataaatgtgt tacgcccgaa cctcatttta caaaaatgaa
    13921 ggggtgagac aatggatgct attaattggc atgaatttgt taactatatg gagaaactac
    13981 tagatgatct gaaaagtttt agcattgctg atgccgtaaa acatgggttg ccagttagta
    14041 caacgatgaa aatgcgaggg ttgaaggttc cggatgaaat tatcaagctg aggactaaaa
    14101 caatcattga aactgcaaaa attgttgaca gtatgaacct tgatttgaaa ggcaaaaaaa
    14161 caacttatga gaaagacttt gtacgttttt taaaaaatgt tatcggaaaa tatctaggga
    14221 aaaagtcttt tactgaattg agagacgaag agattgtacc aactccaatg gttagcaaaa
    14281 ttcgcaaaaa caaagccgag ggaaattact atcttggcac aatagcaaaa atcgttgagt
    14341 atttggaaaa taaagagcaa aagaaaggaa gatagttcgt gtgataaata tgaataggga
    14401 agaatgtacc gaaatgataa aaaaacccga acctagaatt accgaaaaac tgactgattg
    14461 cttgctcttt tacgaaaggg ggcaggcaaa ctaatggaaa aagatgtcaa ttcagcatcc
    14521 actaaagatc aatacaatat cattggagga ttacttggtg ataaggataa gagaacacgt
    14581 catggattac gcattatgct tgcgacattt ctaggggtgt ttcttttatt aacaggcttg
    14641 ggtagctaca tttatcaatt aggaaactat aattattatc ttgtcaacaa tacaacacca
    14701 atcggctcat cattgacgtt tgctcgatca ggagcagaga tcactatctc agatgtttgg
    14761 acagataaaa ctagagatgt aacggtggtc aaacttggtt actcaggaaa cgcaagaaaa
    14821 ttgctatcta cacaaggtaa aaattataac ttgtatatgg caacaaaaga aaaagatcgt
    14881 cctaagaagc ttgaaatgag ttatgggatg ttaagtaccg atggtgatgc tttcttgttc
    14941 ctaaaaggta aattagatga aagagcttat caagttttta ttgctaacca agtatcacta
    15001 atttcaggtg attatacagc tgatgatgat accaaagtca atgtgaatga atcagattca
    15061 gtcaccaagg cactttctca gtattctatg aatgatgttg ataatgaagg agtaatttct
    15121 tttgggaaaa aagcaaaacc agttaatact cctgataata tcaatttcag aataaatcca
    15181 tactcagata caactaacgt atatcaagga agcttcctta ccaaagatgg cgatattgat
    15241 tactcaaaag ttgttgctgt gacaagtaag aataatgttg taaataagct agaagaccag
    15301 ttaaatgctt caaatactaa catcaagaaa atcgaagcca caaaagctga gtatcttgaa
    15361 cgtttacaac aaaatcctga cgataaagat tcgcaagaaa gtattcgtca actagacagt
    15421 cgaatggaga ccgaaattaa aacaatcaat gatacacaaa aattgattga tcagtacaaa
    15481 aatgctgact ttaacaaaga ttcttttgga gatatgaaga caacatttaa gtacctaacc
    15541 ctgactgact agaaacaaat tattcttatt ttagaggaag tgaacacaaa cagatggaaa
    15601 atagtaccga gttctttaaa aatattcaaa ccgaattcaa tcatgctctc acactaatga
    15661 aaccaccgaa acataagtta gtggggcgtg atgacgaact ccatgagttg tcactgatct
    15721 taaatagacc ggaaacacca caagctatct tgttaggtga agcaggaaca ggtaaaacag
    15781 cactggcaag tgaatgggct aaaagacagc gacaagaggg gaagaaagta caagtatata
    15841 aattatcaat cggacaattg cgagttggag gagatgacgt cctccaaaca cgtttggaaa
    15901 ctataattcc aagattgaag gaatacgaac gaattttaaa atccgaggac aaagataatg
    15961 aatttgtcct ctttatagat gaatttcata aagttgtcag ttcatttggg agcggtaata
    16021 aaattggggg agattcgctg aaggaacctt tagcaaatga atcacttcag ttgataaccg
    16081 ctacaactag aaaagaatat gaaaactata tcaaacgaga tgatgcttta ggtagacgac
    16141 tacaaccgtt agaactcaac gaattaaaaa gtgatgtcgt tgtcgatata ctaagacaat
    16201 ggttgaatac atcatctgtc aaagatgatc ctgaagcttc cttgaaatta tccaaaaaga
    16261 ttcctgatga attgcttgaa gaaatcgtac aagcaaacaa gctttatcgt gaggatttat
    16321 ccgaaccggc taaatcaatt gccgtaatgg aagatttgat tgcgaaggat cgtgtagatg
    16381 gcaatgcgta taatcgagaa agcctgttac aaatatttgg aaaatataat attcaactag
    16441 attctgattt tgatatagca aaattggaag aggagctaaa gtccattaaa gggcaaccag
    16501 tagcggtata tacggtcacg atgttgttta aacgaatgaa acttggattt gatcgtttta
    16561 aagggcgaaa gcaaccaaaa gcaacggctt tgtttacagg atcaacaggg gtcggtaaaa
    16621 ctgcgatggc taaagctgta gcaagagggc tatacggtga tgaaaataaa gtccttttag
    16681 taaatatgcc agactataaa actaaagatg atgctatgcg gtttagaaag cttatcggaa
    16741 cacatgtccg gcatgatcca tcagcaatca ttgtttgtga tgaatatgaa aaagcaggcg
    16801 tagaaacact tgatgccttt ttatctattc tagatgaagg aagaatcagt tttactgaaa
    16861 gaggatggga tgatcgagaa gttaatgata ccgtctcact ttcaaatacg attttatttg
    16921 cgacaacgaa cgcaggggct gagatgtacg attcgatgaa ccgatacagc tcactatctc
    16981 gtgttgctaa tcaggaaaat tcaaatgagt acagcactga gcttaaaaga gagtatcgaa
    17041 aggtagcatt agagattaaa caagctctaa ttggtgaagg tctaaaaccc gaagtcatca
    17101 accgatttgg acggattgtt ccattttggg ctttatcgga acaaactctt ttggaaattg
    17161 cacacaatga aatattgaaa agtgctgaat tggtaagaaa acacttaaac gtagacttac
    17221 gccttccacc acagaaaaaa catttctatg gtcaagaatt ttatgcagat gatctctcca
    17281 tgcacttagt ttttgataga gcttcacaag aagattctaa atcagggggt gcaagaaata
    17341 tcttgcatct ggtcgaatca gaatttgaag aagcaattac tgaagcaata attacctatc
    17401 ctaacgccaa aatatttgac gttaaattga gcaaagcagg agggtttgaa gggaaaggcg
    17461 atacggatgc agaaattatc gctaaacctt ccatcgctga aagtaaagaa aacaagtttg
    17521 tgttttcggt tggaggggtg tagctatgaa atttaaaaag tctttacttc aaaatcgtaa
    17581 aattaagcga tgggttgtaa gaggtttagt tatcgtgggg gcacttgcag ttactttagg
    17641 cgctccattt ggaatgaatg atccaacgtc tgatgatcaa ggaaataaac aagaaacagt
    17701 tgcaaatacg aatgtggctt atgctgaaag taaaccggca gaaaaagggg tatggacact
    17761 atatgctcgt atcctgatgg gagataaaga ggtcaatgat agtgccacta aaactggtca
    17821 aatgaattta cttggtcaag gaggtattag tgctgacttt ccttattctg atatttccaa
    17881 acaaggtaat gcaattgccg gtggtaacgc aggtacgcaa ttagcatcaa ccttagcgac
    17941 ttataattac tatgggtata tcgaaacagt ttcaggaaat gcgattgcaa gtcaggcatc
    18001 ctcttttatt ggtggtatcg gtagaacgct tggcagtttt atcggatcaa ttagcttgat
    18061 actttctaat attgctgaat ccatcaatga actccttgga aaatttttca tgagtatgaa
    18121 tatattttca cttatgggtt ggggtaaagg tggatcggat gtttctaatc cggtagctaa
    18181 ggcgttaact agcttactta ataatttggg attaaacaat caattattta aagcgatagc
    18241 aagcttatct atgctctttt ttacagcaat actaggctat caaggaatga aactgttgtc
    18301 gaaagagggt gttaactcaa agggattatc accagtactt gaaaaatgga ttgttcgtat
    18361 ttttgtattg tttgtttttc taccagttgc aggatcgtta ttgggagttt atatcaatgg
    18421 tattcagtca gcaaaagaag atggaaaatt accaacaaat gttgttggtg attacatcat
    18481 taatacaaga ctttgggctt ctactcaaaa tcttgctcca tcatccgata ctggtgggaa
    18541 tgtgccaaac ggaaaaccaa catctgaaat taaatttgtt gacactgatt atgaaccatc
    18601 taaaaaatca gaaatgatca gaatgatcaa tactagcagt tacgctagac aaaataatga
    18661 tctagattcc aattcagtcg gcatggcatt gttaaaagaa tggtcaagta atgatacgtt
    18721 taatgtcaat acttatgccg gtgatgtgaa tgtagctgaa aatgatgctt atatgggaag
    18781 agcgcctgct gtttggacag gctataattt aggggatgga aaagaaggaa atgcaattaa
    18841 gaaacctacc aatttaaatg gttatatttg gtcagctaat caaaacgtta cagacgacaa
    18901 tcgcaagcct gatggaaaag gaacaacgaa taactttgat gctactcaac cttggggtgt
    18961 tgctaacaat gcaacgttta gcacacagtc ggtagcgttg atgctccaat cagcactgga
    19021 tacatcaggg gctaagtttt atgcgtataa tatcgctcca tcaggaattc aaggtagtgc
    19081 taagaatatg tcaacagtaa agacagaatg gcgtacagct tctttagttg gtcatggagc
    19141 tatgggtaaa ttcggctcat ggttagcgat gaacgctgaa tcaatcgtat tagcagtgct
    19201 ttatctagct tgtgcttggg cgatgtttaa cgttaatatt gtaaaagcct tcaaacgtga
    19261 gtttgtgcga gtttgggaag tagtcttaac agggtctctt gccgcaggag caggggttat
    19321 ggcaatctca ttaggagtcg taaccacatc tcttatcgga ttacaattac ctatttttct
    19381 gatcaccttt gttcgtgcat ttggagatac ggctgtagga ttcatcccta acacattact
    19441 tggtgatggt gtagcaaata ttgtacgatc cgctatggtc ttaggcgtag gataccttct
    19501 agtctttaag aaaggtgaaa aggttaataa taatttgatt aatatgctta ttggattacc
    19561 acttgaagta gcgtttggtt ttgacgaacg tgctaagtta ttatttggca atagaaatga
    19621 tgctgttcga gttgtggatg gcgtttctaa agcaatcaaa aaaagccaag gggctaaaaa
    19681 tccttattat gatggtggca gaggatcagt aaaagatatg gtcgcaggag gacttggagc
    19741 agtcggagcg caaggaattg ctgatcgttt gaacaatatg ggagacagca atgctgatgc
    19801 taagacaatc gctgataaag ttgttcctga aacgcctgct tcagtaccat ttaatggtgg
    19861 cggagttagt ggaacaatca ataatacctc tttcaaagat ttagttcgtc aagatcaatt
    19921 acgtaataac tcgcaacaat atagcgatca tctaggacgg atggttgatg aaaaaggaaa
    19981 ctttgtcgat gaaaatggtg aagtattaaa agacagtaaa tttggtaaag atgccaaagg
    20041 aaactttatt gatgacaaag ggcgtttggt cgatgaagat ggaaatttga tagatacaaa
    20101 cggtaaacca ttggaagaca atgaagatgg aaacatcggt atggatagcc aaggacgttt
    20161 aattgatgac aaagggcgct tggtcgatga cgaaggaaac cttgttgatg aaaacggacg
    20221 taaattactt aacggtgatt ttgggcagga tgtcaatggt cgtctagttg atgataaagg
    20281 acgattagtt gatgaagacg gaaatctggt cgatgagttt ggtaagaaac ttggaaaaca
    20341 cagtaacggt tataacgtaa atggcgatac agtcgatgaa gatggaaaca tcattgattc
    20401 aagagatgct cttgtagcag atcgaaataa agaactcgaa caacaacagc atacaccaat
    20461 tgcggttgca atccctgatg atgaaagcga tgaagttatt ttagatcaag cacatcaagg
    20521 gttaagagaa gaagcaaagg ataaagttgt ttcagatcaa gcacaagaag agctaaaaaa
    20581 taaatcgaat ggtgaagatg tttcaaacca atatcatcaa acggctggaa atgtacctgt
    20641 tgaaggtcag tctaatgagc ttcacaacaa ctttgataat gttgctgatc ggaatgatac
    20701 agtcttaaaa gaaaaagaat cattaagaca agaaacagat aaagaccaat caaaattgaa
    20761 gaaaacaact attcctactc ctattggttc aacagttccg aaggtcaatc aagctaacga
    20821 ttctgaaaca aacactgggt taggaatggc gaaagacaat caatccatta gagaacctaa
    20881 aaagacgact gaaagtagca agtcgaacaa gtctaaatct acttcaaata agacagggaa
    20941 atctaaggaa ccatctaaag ctaaatcagc agtagaatat ctatctaaaa ctggtaagaa
    21001 cgtgtatact tcagcagata aagctttagc aaaagcgcca aaaggaagct tagcaggaat
    21061 gacctatgaa gctaagaacg aagctatcaa attaggtaaa ggaactgcta aagttgccaa
    21121 agtaactgct aaaggagcaa ctaaaactgg taaagcagtg aagaaatcta cgatgtcggt
    21181 gaaagattat attgcaaaat atagcgaaca aaaaccaacc gataaaaagt cttaagaaga
    21241 ataaaaagag ggagcattaa tatgctcctt cttttttgtt cttctcttca aggggaatgt
    21301 caagaaaaac ttaattgtct tcaaaaattt cttttttatt tggtagtaaa tctatgaaat
    21361 atttgctttt ttagttatcg gagaataaga gggcttatgc tagttagtta aaatcaggta
    21421 actagtcatt aaatgctgat aaatcagcaa tttttacgaa aaaaaaaaga aaaaaatttg
    21481 agggaaaata cctttcaatc tacaaaaatt tatgttatta tgataacata aagatgtaaa
    21541 tatcgaagca tcttttctgt attccgatca ttgacaagtg aacagagaaa agtttgcgat
    21601 gatataatta tcttgtgaaa aataatcgaa aggaaggatt tataatatgt ttgattctat
    21661 taagaatttg gtttccggaa tgacgccaga aatgaaaacg ttggttcaag ctattctata
    21721 tttaatggct acattttttg cattgaaacc aggattcact gctatgaaac atttcggtga
    21781 tgcaaaatgg ggagaaggct taaaatatgt tggagctgtt gttgctattt ttattatccc
    21841 tgtaattaca actgttatgt tgttaacgat gggtactaaa acaggtaatg acttaaacca
    21901 aaaagctaat atgatctcag ctcttattcc tgtggttggt atttatctag ttacaaagtt
    21961 tccaaaagaa gaacaattag gttaaaaggt aaattgagca aatttggatg atatgccgat
    22021 ttgttcaatt tactttttgc taaaaaacta cactcgaagc gtagtttttg aagtgaggga
    22081 agacaataga tgtaagatgg acagagaaaa gatgttggtt cacgcaaacg aagtattgac
    22141 gagcggtttt aaaggtaccg atttggcgaa gataatgaat atgaacgtaa accagttcta
    22201 tgattatcga aacggcaata aaaagattga aaaagccaga cttgaaacgc tcatcaaatt
    22261 tgaaaaagcg tatctatacg tgttagacaa acagaaaaga actattgacc gaaaaaaagg
    22321 agttttacaa taatggcaaa attcaaagat attaccgaat ctattaatta tgtagcagaa
    22381 acagttcccg tagttgtact ctctttgaag ccacaattac tcgaagtgta ccaaaaagaa
    22441 gaaaaagaag cgaaggcaaa aaaagctaaa gcaaaggtat ggaaagaatt cgatgcgttg
    22501 ctgaccaaag atttaccaaa gaagaattac gtggtgattg aggacgtaca aaagctcagc
    22561 agtgaggaat ttccggatga agaagatgcg attctagatt ccgaaaagta cgttgtttta
    22621 ttaaaatcaa gtctgaatta tgttgttgat ttaccggaaa aattcaaaga aaagctatta
    22681 gctggtgtcc gctttatgga tagtaatggt caattattag ttattcctga tgatcctaat
    22741 atgtttgaaa gagaagttga agaagctaca gacttctaca gtgcagtaga tcaattagag
    22801 gtagctacta aaggcttaga aagaattgct gagaccttca actcttctga tgcggttgaa
    22861 tttgaagctg tagcagaagc cgaaacagaa gccgtaccag atgaagaaga agctcttttt
    22921 gaggataatg acgaagcaac tgaagcagga aaccaagaag caagcataga agaaacacaa
    22981 acagaaacct accaacaacc aaaaattgat gaaaaacccg aatcccagag tgataaagaa
    23041 aattatgttt atgaagctga aaaaatcgta tctgaaagtg atgtaccgaa aaatttagat
    23101 gctgatttgt tagaaaaaga ttttgaacag atcatagcca ataaatttca acctgtagtt
    23161 ttagatgagg ttagtgatcc tgttgatttg aacgaagaga tgcgatccat tgcagagtta
    23221 aaaccattgc tgtttttagc taaagagagc atgaacgctc gtatctctat tgctaataat
    23281 aaaatcaaca gtgaattaaa taaaacgaaa gagcaattgc tactcaaagg aaagcaaatg
    23341 ttagcggatg agcttttgcg gatagaggaa gcaacatcac taagtggtca aaatcagttt
    23401 acaaatacgc tatcggtcgc taaagaaagc tacgaagcag aagcatctaa aattgatgat
    23461 ttagtgatgg aaaaatccag tatcattcgt aaagattatg atatggcgaa acaggcttat
    23521 ttggaagcag aactaaagaa actagagcaa cggtacgatg ctgaacatct gccagaagtt
    23581 aaacaacaag aagatgaatt tagaaaagca actgaagaaa gtcttgatca acaataccaa
    23641 gaagcaattg aatctattaa aaacgatgct ttgaaacaaa atcaagaagc aaaagaagtt
    23701 gtggtggaac ggatcatcaa ttcattgaaa ccggaaattg aaaaatcttt aacagaagtg
    23761 caagaaaaaa cgttagagat tgtcgaaaaa acaaaggcag aaaatgaagc tgagttccac
    23821 gatacaaaag agaaaacaac agatgtggtt gtcaaatatt ttgcgaacaa agaagaagtt
    23881 gaaaagctag tagctaaaga tacgaatgag ttagaatcca aacttgttgc acagcaaaac
    23941 attattgaag attataaaaa tcgagtaaaa gagttcaagg atttagcaga tggctataaa
    24001 ttaaatagcc aaaaattgac tgaacaaagt gaagctttac gagaagattt atctcgtgca
    24061 agaaacgacc tatcacaaat ggcggaaaaa acaaagtcac tagtagttac tacagaaaat
    24121 caaactgagt ctaaacgtga ttttacaagc aaaaagatgg cgactatcat cgctgtatca
    24181 gcaatggctt gtggaacgat tttgggctca tcctacttca ttagtcataa tcaccaagaa
    24241 gcccaagctc aaacagaaac ctcaatttca agagtggaaa attcactagg atcaaataca
    24301 gcttctactt caacaacaga caacaactat gaagcaacac caacgacaga atttaaagtc
    24361 ggagaagatg ttgtcatcag tattgatggg aaaactacta aggctgaagt actttccatt
    24421 gacggtgata aggcagttgt gaaagctcca gatgggaatc aatacaaaat acaagctcac
    24481 ttaactgaaa agaattaagg agggctaaaa tgcgagaaga aaatattgtt tatccagtaa
    24541 ttgaaaatca agcaaataca aaatcttctc aatttcttca agaagtgcaa gtcgtttatc
    24601 cggttaatga actaccacaa acgaatgaaa cagtgagcaa gtcaatatca ttaattggct
    24661 tgcttattgt tgcaacagcg ctaactgttg gtgtttatcg taagctgaaa gtgaaaggaa
    24721 gtatttctga tggcagataa ctcaaactat ggatttaaac ataatttgca tcaaaaacct
    24781 tctgaaacaa tagtcagagt tgattggctt gaaaaacatg tcaacagcac ctattctgtt
    24841 acagcaaaaa gactaattgt ttcagctatt gttgttgcag ttgttgttct tggttcaatc
    24901 gtttatttgg tttccgatac gattaaattg agatttattt ttattccaac gctattgatg
    24961 ataattggtt ttcttatact gattagtcag tatatgttta ttgatccatc tagcggatca
    25021 ctgatctcag gctctattag actgtttaaa tcagtagtta acacagcaag agtaacgatg
    25081 gggaaaaaga aaccatttcg aggaacggag attttgaaag ttagacaaga tggaacgatt
    25141 atgtatcgag atggtagtgt agcactaatg tttaatttgg atggtgctac ctcgttaacc
    25201 gccttcccag aagaaatcat agcgcaagaa ggcaaatcaa ttcaatacca aaatgcaagg
    25261 gatcgttcct tggctgaaat caaaattact tcatcacaac ctcagaatac ggaacgtcaa
    25321 atacggaaca tgaaagtaca aaaaagaaat gcaaagagca acaaagctgt gtttgacata
    25381 gtagatcaac aagagaggtt tgtatctgaa tatattgatg gggtcaaaac tacggtgatc
    25441 caatatctta ttttgaagtc acaatctcag atggtattgg atgaaggcgt agaacgttta
    25501 gaaaattaca cgagtagagg gctttactat agcgttactc agttgtcagc taaacaaacc
    25561 aaagaaatat tatcagaaat ttatgcactg aaataataga ggtgagaaat ttaatggaac
    25621 taatgagtag atcgaagcta cttaatttca gaaaaaagaa acacaaaaaa gagcaaaatg
    25681 ctgatagttc taaagcggta aataatactt atgtcagtga tttaacatca ttggttggtt
    25741 atcaatttac accatcttac attaggacag gtaatcggtg gggaacgatt gttaaattag
    25801 tgaatacgta tggaatgaac agaaattatg aatttggatg gatggttaaa gctattcctg
    25861 aagtaaaggt tgaaggagta aaagcttact taatcgaagc ggataagcca gtttcgcaaa
    25921 aagatcagca agaaatcatg cacgaaaatg tcggtggatc aacgaacagt tatgaaaata
    25981 acttgaacga taaaaattca gatgcagaaa tcaataaaac tgttatgcga atcattgatt
    26041 tgaatacagc atctctgctt gacagtacgg caaacaaaat tattgattgg aagttagaaa
    26101 tcctcttagt atccgattct cctaacaaga tcagcgaaca gttgatcaaa ctgcaacgcc
    26161 tgtatgatga aaaaatgtat ggattaaaac ttatgtcttt tggtggcgat caacgtgatc
    26221 tgtttgatag gcttttaagt gagccagtcg gtagtgttta cgaatacaca tctatgagtt
    26281 ctgattttgc aggaaatgat catgctattc gtaaaggctt agatgatgca gatggatggg
    26341 cgatcggtac gatgtctaca agctacgctt caggtgtagc gtttatggca atggatcggt
    26401 cttttaaggg taaagtctta gtagcatccc acaaagatgc aacaatcaaa ggctataggg
    26461 aagaaatctc agcatcatca ctttgggggc aattgattgc taataacgca atggcaaatc
    26521 ataaacggac gtttcacatc gtattaaata aatttgacta ctttggtgat ccgacagggg
    26581 aaacatttgt tgctaagcca tctttaaata gtgtgataga gaagattaat ctagctgaag
    26641 aaggacttaa ttctttagag ctttttggtg atcgaaagga tattggagat ttatatagac
    26701 gtaatcttga taaactaact cagattttct atctgttagc ggacagagaa ttaaatcctg
    26761 caacaagagt gttgctgaag aaagagttgg ggaactttta tattagtcag aagctttggg
    26821 atattaacta tgaagagcgg tcgaaactgt tagtaaacag cagtgataca atgccggttc
    26881 ttggagattt tatcagctat ttaacaaatg ctgagacaaa agcgaaaaaa accggtacag
    26941 aaaaagaaat tgatcggatt attgtattaa aagatacatt gcgaacagca ttggatagct
    27001 tctctaagat gttcaatcag agaactacat tgaaagaaat tggcaagagc gacaaattgc
    27061 agttttacta caatttgagc gatttaagaa cagaaatcaa tgtcatggaa gcccaatttt
    27121 taaatacctt tgacttcatc acgtcacaag ctaaaaaagg cgatatcgtt atgatccatg
    27181 ggtttgatag attatcagtt gaaaccgttg gattaattaa aaagcgccta acaatgcttg
    27241 aagaagatgg agttcggctt gcgtttctct ttgatactat cggatcagga gaagataaag
    27301 acggtatcga aacagctgat attttcaata cagatggaaa aatttatcaa agttttgaaa
    27361 gtgattttaa ctacactatc atcgggaaaa tgaccagtga agaattgatg aagtatgaaa
    27421 agaaggttgc tcctgcaaga aaaggagaag atgatcaaaa actatcagtg gatttgagac
    27481 agtatttgac atcaggtcga agcaataaat ttcaaatcag aagaccagtc gatttgacga
    27541 acaattttgt tattggagag tttattattt aagttctatc cttatggtat cggaaaattg
    27601 tgtctagaag gaagaaatat ccgtcaaaac aaaccatact ttttttggaa aagcggagtt
    27661 ttagaacaat ttcctaggag aaatagataa aacaaaaggg gcgaatggtt atgaaacaat
    27721 atttaaatga atggaaagtg atagaggggt cattagtagc acaaagaata aagggattac
    27781 ctgattgctt agagaaggat catttgtttc aaatacgaga aatgttaaaa aaagaacagt
    27841 ttgatccaga ccaattttta gtagtcgagt atccaactat tggggtgtac tgttgtaacc
    27901 atatcaatga tgaaaaatat tttattattc aggaatatga aggacaatta actcctttct
    27961 atactacatg ggaaatgagt gaggatggga tcaataattt tccatgtgaa agcattgaag
    28021 agtcaatatc tcaaaccgaa tgctaattga ttataactat aagaaaggta gtgagttaat
    28081 ggatacagct atattgtata ttaacataat tccgtctata tcctttatca ttactcttac
    28141 aattttcata cgtagaaaaa aattttttac agagaagaag cttgtatgct ttttgatgaa
    28201 aagtggagta gtatttcttt tttcttggtt attgcttatt ttatcaactt caagtttttt
    28261 aattatatta tttgcgactg tgggtcttat agactttggt gtaatatcag gagcagtaat
    28321 gtggttttgg ttaactagaa aacctaaaaa aaggaacaag aaaaatggtt tggaaaataa
    28381 acgattattt agattaatga caaatcatta aagctatagg catattaaga gataatattt
    28441 accaatttcc tgattgtttt gaaaacgagc atctgtatca aatcaaaaac atgatgaatt
    28501 gattttgtta tattgaaata gatattaaga aaaagcattc cttgttaaaa cacaagggat
    28561 gctttttgtc ttcacttaat ctgtttaata taatctaaca acttttcttc ggaattaatt
    28621 ttttcaccta ggtattgttc agacttatta acaagattca atttaagatc atttaatatt
    28681 tctggatacg tatcactttt cgattcagct aattcataca gtttttttag ttccttatct
    28741 tttaactgaa acttctgatc catagtgaca gcatttggat atagaagtcc attataatgt
    28801 gtgcgtaaat ccatgtggaa tttttgttta taaaagtcta gcagattaga aataaaagaa
    28861 gaaaaataac cattatagta aaactgatta tttctattta agttatacaa caatttatta
    28921 aattgatcct gatttaattt gtcgtaaatc catggcaata tttccatgtc cttatctgag
    28981 tatctaaatg caggagaatc ggtcagatgc tcaatcataa aatctaagaa catctctttt
    29041 tcaccaaaat atagatattg attatatatc gtttctaagg tagaagttgc tagagaagag
    29101 actctcatgt tttgctgaag tattggatca agtagtctat ttaaatgttc ttgtaattct
    29161 tccttggtgt ttcctagaac tgcaagtgaa cgtagacggt agtaatcttc tatatgtgtt
    29221 gttattggct gttttggttc aaaatccttt attgatattt ttaagttttg aattatttct
    29281 tctgaaaaat cacgctgtaa ttctggatga ctagctataa aggtaagcaa aaagaagcct
    29341 tttgtataag taatctgttg attttctcca agtgatttat taatttcctc tctcgattca
    29401 gctgtgaacc tgtagtttga gatttctttt tttagaattt ttaaataaaa ttctttgtta
    29461 tcaaaaatca gggcatctag tagtttgaaa gtacttactc tatctctatc tacttcttca
    29521 ttcccataag gtaatttaaa aacaaactta aaggctgttt tacaaaggtt ttctttggct
    29581 ttctcactta gttttttcag atagctattt tttagtgcag agtggaattc ttcaggtgta
    29641 acagacatag aatgtattcg acttgcatat tgtatgattt cgttcgttac tttttttcct
    29701 aatattggat tttttagaaa tacaatttca taggatttcc gtatcacatc ctgacaagtt
    29761 tcctttgtta tttctcttaa ttcccaagtg tcaccattgt aagtgactac tggatgagct
    29821 gaataatttc tttgatcctt aatatatttt aaatcctcat attcgtagct atcaattagc
    29881 tcagtcgaat tataagtttt ttctagtagc tccatttccc atcttgggga gtttggatca
    29941 ctttttctta atttagctat ttcttctaaa attttcttag aagtatcatc ctcgtatata
    30001 tttgctaaat cagataattt atcaattaag tctaaaacaa gcgcactgta attgacagct
    30061 atcgctgaac gataatttcc ctgataaaaa ctacttctga cttcttctaa taaaggaaca
    30121 attctaggat ttttaacttt gtttatttca tcatctaata taaacatata actctccttt
    30181 tttgttgata tgacaagttt accacaaaaa ataaattaaa aaacgagaca tatttatgtt
    30241 attatggtat cataataaca taaatatatg gaatgagatg atgcaaaatg ggcttgaaac
    30301 aatcagttta catctggtta tatcctgata atcccatgtc aataatggct gatatttttg
    30361 taggtagcat tttattgtgt ttactgtatc taattttctt ccacaatttg atcgggtaca
    30421 tgatccttga ttggaaagga atgttcgata tagaaagaaa accatttaat aataagcaaa
    30481 tttataccac ctttgcaggt ggagatttgg aagaaaaaga agttgatgtg acacatgaaa
    30541 ttggattagc tttatcagga attcaagatg ctgatgtatt aggatcaatg ataacggata
    30601 ccggatataa cgcctatgaa gtattttatc tcatgtattt tatatggcga gatagtcaag
    30661 atattatcaa cggtcgtgct gaaaaaacaa tcatttcaaa tactgaaatt tctttctgta
    30721 tgggtgaagg ggaaatgatt gtatataaag atggatcagc aaacattaat accgcctttg
    30781 gtgatattga aactagtatt aaagagctta gggagtatgt acagaatccc tacaattttc
    30841 aatatttagc aggaagaata aatttagttt aggagtaaga atatgaaaaa aattattgat
    30901 cagccaacaa atcgcttaat agtcgaaggc gttattttcg taacaatgta tttcactgtg
    30961 ggaaatggta tttcaaatac aatcattcaa aaagacccac aatacttgtt gtttagtctt
    31021 tatgcttgga ttgtgcagat gctttgggtg tctttattaa tgctgttgtt ttattggata
    31081 gtacgggtta ttcttttagt gttaaacaaa gcaaaaaaga aaaaggatag gtgagtaaaa
    31141 atgaaagcaa caaaattaaa acttgtacta ttttcaacag cagtattact aactttatca
    31201 gcatgtggta catcaaagag cgctgataat accaaagaca caaaatctag cagtacagtg
    31261 gtgactgaaa gcagttcagc gcagacatct acaactatgt cttcaatgac tgagaacaat
    31321 gctgaaaaat ttcaaaagta taaggacgta aatcaaggta aaaagccgga agaagtttat
    31381 aacgagttta tctcttcagt aaaaaatgat gaaagttatg ggaatgatcc attacaaaat
    31441 aaaaatctga ctattgttct ttacaaacct gattgtcctg attgccaaaa agcagaatcc
    31501 aaaattatgc aagaaataca agaaggattt agttatgctc caacattaat gcacgatatt
    31561 accatcggca aacctgctta cgaatataat gcagttgcgg tagatataac agatggcgta
    31621 cctgaatggg tgcaaaatat ccctggtctg gctgatgaga acaaccaata tcacacgcca
    31681 acattagcag tagtaacacc aaagaaaatt gatggaacac tatactggga tttaatggga
    31741 atgacaacaa atacatccga tgaaatgatt gaaagcacca gtaactacta tatggatttg
    31801 ggagcctata aatcatacga acagtctgaa cctgttatgg atgatcaatc tactattaag
    31861 tagtttttag ggcgtaagcc ctattttttt aaaattatgc gataacaaat taatgatttc
    31921 tgaaacaaac gttcttactc aactactcga aacacacaag ctaattaact agattataaa
    31981 ggagaatgcg taatgaaaga aatcagtgca agtgaagccc atatttctca aaagaatcag
    32041 gttggtatat ataaggtgct tcatggtgaa gggatttata aaatacagtt acttactggt
    32101 tacaataacg gtgcaaatgg gaagtggtta aaggcaacaa gtattcgtga acttagaagt
    32161 gaagtagaaa aaattaggct tggtctcaca agtgaagaaa tagctaaact gaagggaaat
    32221 aattcttttg taattgaaaa agaaaaggaa aaacatctac tttatcgtga gctgaaaatt
    32281 gaagaatact atgaaattga tttacctgaa aaattaagaa ttattgatca acgttcagaa
    32341 gaaaaatgga aaactgaaat atatccaagc ttaaatgact acaagacatc aggaaagccc
    32401 atgtataact acacaggctt tttaacctac acacaataca aatatgcctt tcgcaaatta
    32461 aaaggcatta aacaagctga aattgccctg tatgagatga aaaaagtgga tagattgaaa
    32521 ttgattcaag agttagtgca aaatacatgg aatcaagcga ttgtcgagct agaaaaagaa
    32581 aatgggaaaa ttttaactaa taaatatctg atcgtaaatg aaattgaaga attaattggt
    32641 ttattttcat gtgaaaaagg tgaagtcttc ttgaagtcat caccaaccta ttcaattttg
    32701 gacagtccgt atcagtttat acaggcaagg agatgagtta gatgtctagc ttgaaagatg
    32761 actatatcca agcaaaacaa gagtatgaaa aaatacagat gttgaaatct gagaaagtcg
    32821 aattattttt aacacaaaaa ttagcagagg atcatattgc taacttagat gctaaaactt
    32881 tagaggaata tgatcggcag tttaatcaga tacatcacaa tatcattttg cttgttgagc
    32941 aggcaaagca aaggtttgaa aaagcggaat atgatctatt agatcaagga attgtatgct
    33001 tggataaggg attgcgaaga tattatggtg cagagttaaa aaagcaaaat gtagatagaa
    33061 atgcactaaa acatgaaatc ttagaattga taatgcaacg aatttgaagg gatagaaagc
    33121 aaatgtcaca aaacattagt gctgtaaaac aattaataga agaagattta gagaccacag
    33181 ttcagagatt agcggaacga atccattttg caaattatca aaccatctct aactggtcaa
    33241 gcaggaacag aaatccggaa gaaattccaa tgaaattttt ttatcgaatc ggaaaagaat
    33301 atggtgttga tccgataaaa attttcgaga agaccatgat ttattactat catggaacac
    33361 ctaacgaagt gttagaacat cttttggaag aggcaaaaga aaaatcatca atattagagc
    33421 tatcgcaaaa tgacaaagtt attcaagtga aatgcgttga tccaacagga aagattattg
    33481 tcggttttga tattgaaaga aaatccaaaa tagattttat tactcaatta aaaaagataa
    33541 ataatttaga tattctaacg cttagaccag atcatgaaca gttgtttatg agttttaaaa
    33601 aacaattgaa agaaatacaa aaaagatata acaaggtgtt tcctaatgcg tgaaaagctc
    33661 aaagaaatca gagttttttg gataattaac aaagaattta tcatcttata tactcttgga
    33721 tttctcagca tgatcttgtt ttggtggtta tatttaaaaa agtaattgga gagggtcaag
    33781 aggattttta tcaagataag gatgtggaaa ctgcatgtgg aataatagat taaaaacagg
    33841 attgttatta atagttattt cctgcgctat gatgattggt atgaggatac aaagggaaca
    33901 atcctatttt gaagtttctg ctaacaacgt aatagaaaaa tgctattacg gtcaacatta
    33961 ttggagtgag gaagtcagag aaaatataga tcgtgaatat gttcaaagaa ttgtttggga
    34021 tgcatattct atcaaagatt atcctaaaag tttaacgagt agattatttt acagcgaaaa
    34081 agataatcaa aaactctcag atttgatgat gaaaaaagta aggaaacttg cacaaagcta
    34141 tttggaagaa aaagcaggag taataaaaga taaggaataa atggagaaat ttgctattga
    34201 atgaagaaac ggaattattt ttaaaaaaag tgaactttct tgtagaaaaa tattttttaa
    34261 caaagcacaa aattatgtat ctaataaaag gattgatcgg tggaattatt ttatggatac
    34321 ttactttctt ttctttattt ggtggattta ctaattataa aaagttagta gacgaaaatg
    34381 tgaagcaaat tagtgcatct gaatcattaa gacatcaagc tgaagattta gtaagtaatc
    34441 cattatttat aggaggtatg gtagaagtga taataattag cttcgttatt ggttatttag
    34501 gtatgtgtat cttccagata atctttgatc taataagagg aaaagtcttt caagaattta
    34561 aatctgaaaa aataaattta cgttagagat aataggctat gagaggagaa gaatagtgga
    34621 acaaactaat catattactg aagaaactag gaaattcatt tgtttagaat cgttttattc
    34681 ggaaggtagg tattgtaaca aaggtgaaac ttatacggca tatcctattg aagggggatt
    34741 taaattagtt tttgaaaacg gagacatgaa ttttaccact gagttgtttg agtgtgttct
    34801 tgaaacgtgg tcagatgtgt tgcttgaagt cactaaataa tcaatgttat ctagtagaat
    34861 taagcaacta aaaaagaaag aaggaagaaa atatggcgta tgaaaattta caattagtag
    34921 tagcaggagt tagtggcgat atatatcttg caaaagcaaa agatggaatt atggacacga
    34981 actatagaag agttgctaca aatgacgttt tagtagcaac gacagaatgg ttcattagaa
    35041 ataacaaaaa gttaatccaa tttgaagcag atgaaaatgg tagacatagc ttgttttata
    35101 caaacgatca aaaaaaagct gatcggatca tagcgattct aaaggagaat gatgatggtg
    35161 gacaaagaga ttaagggcga aataagatta tttcaaagag aaaatgacga ttggggtacg
    35221 gaaatatcaa agaatcttgc tgaagatata gagttacgat tgagctttat aagtatgata
    35281 attgaaagtt ttgcttttga gcttggtata ccgcaagaaa agttagcaga aatactgatt
    35341 aaagcaagta acaatagtcc tcagcacttt cacattgatc taggtacagt aaattagtct
    35401 ttaatcatta atattaattt ttttgataag ctgatctgat taatatttct taatgtaatc
    35461 attccaatat tgatcttcag ccgttaacgg aatatataga ggaaagcggt gaaatgaaat
    35521 gtctgaaaat catttgaaac gattatcgaa aggaacggca agtatgtttg atgccaatct
    35581 actaatcttt aaaagcctgc tagaatttga aaaacaacgt gtagaagata ttgctaagta
    35641 tggtaactat gctactgttc cgagtgttta caatgattta atgaaatggt tcaaggtaca
    35701 aacagggttg gaatatagcc aacagaattt agtggtaaaa atagaggagt atgaaaatca
    35761 tggctaaaag tttatcacaa gaattaagag aatttgcttt tagagagtga tgatcctata
    35821 gctgaactag atcaattagc ggaaagagca aaagaattag aaatgatttc tggtgcgtat
    35881 agaattattt ctgaagaatt aggtcaagcg gttgtgtcac aagcacttga aaattatttg
    35941 aaaaaacaag ttgattagtg acagaaataa cggaatagga tataaatgat ggattcaaga
    36001 tgatattagt taataaaaaa tagtatggag ggcaactaat tatgccagta aaaacaaaca
    36061 ctgaatggtt agatatatgt gatgaactat ttgatactaa agacaagaaa gaactggcga
    36121 tgcgtgtagc atatgctata gttcaaaaag ataaatattt tgctgttcca tctacagact
    36181 atgacattga agtgatagtt gaccctatta ctggagataa gaaattacga gtagttattg
    36241 agtgtaacgc ctaatgtatt ctttagaaca ataggaggaa tcttatttct taaaagtgca
    36301 ggaatcatat agaagagtgg ttgctaaccg tcttaagtat attagaaaga gatgcaaatt
    36361 taatggatat ttttgaagtt aaacctgatt caggaaaatt tattgttttg caggaaaaaa
    36421 gagtgattgc aattgaaaga acagaaatag aagcatataa aaagatacat gaactagcag
    36481 aacaatatat tgaagatttg aacaataatt ttcaagaatt tctaacgcaa aatgaatagg
    36541 caggtataga cagagataac aaactaggaa aggaagacca taaatgaaaa tttttgaaat
    36601 tgatgacccg tattttgcac ttatagtagc tgaaaacgaa gaaaaatgta tggatatata
    36661 tgaagaagta atttgtgatg tagaaggcaa agaagaattt ttaccttgta taaaagaact
    36721 tgaaacgaca gttgcgatta caaaggttgc aaatgcagtg agtaatgaaa caaacgagct
    36781 agtaggaatt catgaagctg gtaatcttgt gtttagttgt cttaatagca ataaatctgc
    36841 attattggct cttgatgggg atttatttta atccaacaac caccaaaatc acctaaaatg
    36901 gtcgatttga ccagtttgga taaataattg tgtagatcaa ttttgtaaat gaagatgtta
    36961 gataaactag acatcaatct gtatagctaa agaaaagaga acggtaaatt atatccgctc
    37021 tcttttcttt agctatttat agaatatacc ttctctaaat ccttccatca atgtgagctt
    37081 gcttaattca tttgaaatta aaaaagttat tgctattata cacatcgttg tgtataatat
    37141 aaagtgtaaa tataattgtt cctttgagct attcattaga gttgaagcat agaatagttt
    37201 catagaatcc atagtaaaaa atttgagagt aaaggagaag ttatgagcga atttgaattt
    37261 ttagataaaa gtattggttc tgcttttttg attgatcctg atctgtttgg agcatgggct
    37321 caatcatcag gttataattg ggcttttagt gatgatatgg tcgagctttt aagtcaaacg
    37381 atgaattggg aagcttataa gatcaagcaa tttttagatg ttgttgcaca gccgatttat
    37441 acatctgctg atgatcattg gtcaggtaga gagttgacaa cactcaaaga actagcggag
    37501 tacaataaaa gatacaaaat tccgtttcaa gtttcttaca tgtcagatga tttttccgtt
    37561 atttactcaa taggtaaaga aagcagatat ttaatcccta ataggcactt agagataaag
    37621 atgaatagcg attataccga ctatacgcta agtgagttaa aagctcttag acagagcgac
    37681 agtgaggaat ttagcgcact caaaaatgaa gtgagtgcag ataattttcg cagatcagat
    37741 attgaagaac tgaaaaaaaa gcaaaaacag caactagaaa agcaaaaaaa attaattgaa
    37801 gacattgaat ataaacgagt agaagagctt gcaatacaag aagccaaaat tgaaaaaatg
    37861 cgacaagaac tctataaaaa acagcagtca atgttagcag aagcaaataa aatgcggttt
    37921 gaattagaga aaaaagtaga agatttgaat actaaaattt tttatttgga aactgagatt
    37981 aatgcaatcg aaggctattt tggtgagagt acaacttttg ttcagttgcg ttcaggtcaa
    38041 aagagcttgc cggaagaacc agtggtgatg tttcaaaaag tacgatatct agatgaagaa
    38101 ttaggaagat atttcagtat gcgagaagtt gaaggtacag ctgataccat gctgactttt
    38161 gagcaggcgc tatcctcaag agatgatttg tttgatttat tctcaccgaa caaaaagtca
    38221 atctcaatga tacgtatttc caaatcagga tatggctaca ctaacggtca aatggaaagt
    38281 acaaacatgc tagaaaagta taaaaaatat catggagaaa ctttagcaat tttactgaga
    38341 aacggtgata acttatggat ttgttggttg gatgaaaata agatcagttt caaagatgat
    38401 ttgttttacc aaaacaaagt aacagagcag gcatctgaaa ccaaaaatat ggtaaaggag
    38461 atagattgga cacatactcg taatttagaa gagcgattga aagatgctaa aaagcagaag
    38521 aaggctgtta gaggagagtt tgctagtcgt tatattcttt tttcaatttt gcaaggtgtt
    38581 ctagataatg gaacgatgat gaccttacca gaaaaagtaa atgtgatcca agcaattaat
    38641 cagaaaagcc catattttat tgcaagcgta gcggatggat ggttagaaga cacacgcttg
    38701 ccgtcattta gcgagttgat tgataaatac agtttctata aagaaacagg tgaagacaga
    38761 gtattagaag cggaatatga gttgaatccc gataaatttg aaaatatgag agattatcca
    38821 cacaaacaaa atgatgacat ttatgtgtta taccggttac gtgggtggtc gcctacatca
    38881 gcaaattaca cttattacaa cagtgataac cgttcaaata gtgagagtgg acgtgactta
    38941 gcatattcga ttgacttgaa agacggcatt tatcagatca atttagtaaa acagggaaaa
    39001 ttccaaaaac aaatgttttc ccgaacgttt gttggagttg attctactac aggcgaaaat
    39061 gtctatgatt actattatga aagagaaaca gagtactata ttcgaggaca aaaagcctac
    39121 aacaaaagtg actattgggg tgacaaggcg aagcgattaa ctgcaaattt tagagtgtat
    39181 gaatccgaat ttataaactt aacctttctt aatagtgttt ttattgatta tgtcattact
    39241 acgaaacgta ttggaagtta ttcaagtacc tacgcatcct tagttccaat gttaaactcg
    39301 atgagccgtt tcttaaaaga aagagaagag aaagagctag agtatatcaa agaaattatg
    39361 ccagattttg aaatgcacga atggcaaatt gctttatcag attggaaatt gacgaataag
    39421 gttcgtagaa taacaccttt tcaagccaag cgctttttga aagctatgaa atttgttcaa
    39481 tagaaaaatc caccagacaa gctgatggag actaacccaa ttactaacag ttgtaattgg
    39541 tgttagctta taaattaatt atggtgttta gtcaatccca aattgtgaaa cggcaccaat
    39601 aattggaaag gaaaaatgaa atgtctgaag atttacgaga gctgaagtta cgaaagaagc
    39661 agtatgacca gtatttttcc ggtttgttga ataaacatat tgaagaagga cttgatcatg
    39721 attttggaaa agatgaatat tgtttgtatt gcgagcgtta caatgaaaaa atgtctgaat
    39781 atctacagct gaagaaagag atacagaaat tagagaaaaa agtacatgct tggaagtata
    39841 aagattccat gtccggttat ggtgaatctc aaaataagcc aagaaaaaag aagcaagcgt
    39901 atgttctaaa gatggtaaat ggggaagagt taattgtatt agggtacagt aaagaagagg
    39961 ttcgagaact cgttgaaaca cctgtcagga aaaaggtaga aacaataaat gagatagacg
    40021 acagccaata caatagcatc tatattgaaa caagtcatgg tggatataag aaattagcaa
    40081 ctttccttag aagttctaaa gttgggataa tttctaaaaa agacataagt gctgttaccg
    40141 atagtttttg tactttatct gttcctaaag catattttca atacaacagt aatagatttg
    40201 atcgcttgga gaaattctgt agaccatata ctgtattgaa aactaaaaaa ttcaataaaa
    40261 ctaaaagagt ttcaatcaag ttaccgactg gtcaaatctc gctaggcgaa aaagattatc
    40321 tgataaaagt aggaaaagag ttaataatca aagtgccgga acgcttattt aaagaaaatt
    40381 ttgagatacg tgaaaccttg gtatggaaag aagaataata gtttggaaga catagcaggg
    40441 tataaggagg aattgaaatg gcagtaaata gaaaattgca ggtattgatt acagacgact
    40501 ttggcggagc agagtggttt gatgtgcagt ctatagattt tgaagaagaa gtcgtagtta
    40561 tagaaaatgt atacggcgaa gaatactcat attcgcttga aggagagaat gttgaggatt
    40621 tacggttgat tggtgagtag aaaatagcag aaatatcaaa atggagatga aatcagcgcg
    40681 tatatgatta acaagaaaac aggaaaacgt attttcagca gtaaagacga ggtaattcgg
    40741 gatttgagtg aaaactttga accaattgaa ttatttttcc atcagttaaa tctagaaatt
    40801 gaaaaattac taaaggaacc tacagaagga cgaattaata aagtattgaa attaatagca
    40861 tgtaaagata gtcatgctta catgctttat ccaaaatggt atgcagacaa aaatatagct
    40921 gaaataaacg attatctagc tagtataaag gaacgcaatg caattaatta acaatagacg
    40981 aaaaatgaaa ttaaaggaga taggaaaatg gcagaagtta aatttggatc aaaagaatgg
    41041 tttgaagagt taaactcaat gattcagttt ccagaaaagt tcacttttta ctacaacaaa
    41101 agagacaata tcattcaagt agctttactt gaaactaagg gaagattaga aaatactcga
    41161 tggagtatga taaaaccatc agcactcaga tttcatccta aagacttgac agcggttgta
    41221 gcggtaaaag aggttcaaga ctcacaagaa tactatgaag tagtaaaaaa tctgaaggat
    41281 atctggtgta aataattcta aattgaccag ttatttggtc aaaaatagta tgtgattatt
    41341 tcatccaata tggcatcata attggtatga aaggggataa attatttgaa agaactagaa
    41401 ggtattaaga aaagaaatca ggaaagattg gaactgttaa atgaaaatag aaataggatg
    41461 tttagttact atgaatccat gaggtgttta aataaatcta gaagtgaaat tatagaatca
    41521 tcaaagtata aacaattgat ggaagagaga gtttctctca ttcaagaaat agaaacagag
    41581 tctatttttc cagatcattt tcctaaagtc cgattttata attaaacatt aacaaatgaa
    41641 acgatgctaa ataggcatcg ttttttattt atttgttaaa tttatttatt tacaaaatgt
    41701 gtatcagtgt gtataatata tttgtagata agttaagaaa ggatgatctg aaatgaaatt
    41761 agaaggtagc ttacaaaaca gaatgatgga aggcagaaca acaaataagg agattgtagt
    41821 tggtatggga gcaaccgagc ttctatatac cgacagaaat ccttatacag tgattgaagt
    41881 aaaaagcaaa aacagaattt tggttcaagc ggatggggca atcaataaag ctacttttcc
    41941 agatcaagag tgggaataca gcagaaatcc tgaaggtcaa atattggaat taattaagac
    42001 aaaaaatgga tggaaagtgc ttaaagaaga tacttatttt tatataggtg atagagaaaa
    42061 atactacgat cctagtttat aaacaaattg aagatgagcg aggttttatc ctcgctcaga
    42121 aaggagaatg aaggatgttc agtcaagaag aaatcatcag caaatcgcaa ccggataaaa
    42181 atggaactat aaccatgaag aaaaaaattc cttgctatcg ttgtagcgga tcaggtcagt
    42241 acaaatttat gtctatgggg aaaattacct atggcacatg ctttaagtgc gatggtgacg
    42301 gatttcttgt tgtaactgaa aaatcattta cgccagaata tagagctaag ctaaatgctt
    42361 taaataaaaa aagggctgat cgaaaaataa ggatcacaag agagaaagaa tctcagaaaa
    42421 attttgatcg ttatggtttt gaaaagggtt atatttacgt ggttatagag gaaaatagct
    42481 atgcgatcaa agatgaactg catcatcaag gagctactta tgatccttta ttaggttggt
    42541 ttttcaaaga aaaacaagaa caatacaaaa ctcaaaaagt ttttgtagaa gaattttccg
    42601 taatcaatga tgaaggaatt attgaattga agctagaaga atacagcgaa agacaacagg
    42661 agattttaag ggcgaagcgt agagaagagg gtgagtttat tggtacaatc gctcaacgat
    42721 tggaattgga tttgaagctt accaataccg tcaccataga tacacaatgg ggcaatgttt
    42781 atctgcattt atttgaagat caaaaccata attcattgat ttggaagaca aataaatttc
    42841 tttattatga tgaatgggat gttggagaaa taaggaaagt taaagcgaca ataaaagcgc
    42901 atgattacta cagagatacc aagcagactt gggttaaaaa tgttaaaatt caaaaaaatg
    42961 agtcagaagg gataaaagta tgaaaagaga ggaatacaaa caaagattaa acgaactgct
    43021 tgaagaggac gaaacattaa ctcatggttc gcctgatgaa attctataca tgattgataa
    43081 tatggtgatc tttggtggat atgaacttgg taataggtca gttgatcata atattttaga
    43141 atttgatgat gtatcatggg aagaaattct tgattgggga attttggcgg tacctgaaac
    43201 taaaacctat attagtgaca ctatggttcc tttttttgag gaattggatt ataaaagact
    43261 tcccaaaaat gaaaatcata tactgggagg aaactaacaa tgttaagtga cttatttatt
    43321 ttactgatct atctaggtgt aagtgaaatt attgcgtatt ttttgtttgt cattgctaat
    43381 tatggtattt ccagtgcaat ctttcaaagg tttagattac cagtaagtga actaaaaaaa
    43441 atgatgagat tatcagcata tcttttaatg tttttgaatg tggtcacgtt aatctttgta
    43501 gtaatcggtg tgactttcaa tttaatggaa tagagggaaa tctataatgg aaaaaagaga
    43561 aaagaatgaa catatttttt tgaatcaata ttgttgatgg tttttgcgat cagcgcttac
    43621 cagaaatcta aaaaaagagt aatgattgtt taatcatcac tctttttttg tagggaaatt
    43681 gaaatatgtg gagcttgtat tggtttagtt aggattgctt ggctataagg agctagttct
    43741 ttatcaaaac catcaaagta ggttttcagt agcgatttaa ctgatcgctc atcttgtttg
    43801 atttggataa tttcagcatc caaatcggtt gtgagatcag gagcttttga tgcgattacc
    43861 tgattttttt tatcttccaa ctctcttagg ctgtttaaca agttgtaaaa agaatcattt
    43921 tgattgttca ttccaacgtg agttgcaatt ttaacagaag tttcgtccgt attgtcatca
    43981 aaagaactca ttggaatatc aagtagtaac gaggatgttt cttttactga ctttttcttt
    44041 acagcatttt ttgtttttcc atcttttttt ctctttactc gagatgtttt tggagctagt
    44101 ttaggtaaat cacttgtttt atcagtcatt tattagcttt tcctttctat taatttatgt
    44161 tattatatta tcataataac ataaaaaagt acactttttg ggggcgtagt tattgaaaaa
    44221 atctaagaga aagattgcca cgttgcttat gttaatggtg caatttacag ggattgcaat
    44281 gagtgtaatc acaataaatc cgcaagtgat acatgctgat gggatagcta cttatgatgc
    44341 acaatttaac tgtactactg aaggtagtgg gtcaggaaca gatagcgatg gaaacgatac
    44401 tggaagtggc gctgtatcag gtgatgtagc aaaaaatatt gatgatattt acaaatttat
    44461 gaatggggaa tatgggctat caggtgctac tatcgcaggt attctaggta actggaaaca
    44521 agaatcaggt aatgatcctc gaacggtaga aggttattac ggtgatggac agagagaagt
    44581 tgaatttatc aaacaattca ctgatggtct gatgggtatt ggctatggtc aatggacagc
    44641 aggtcgccat actgcattag taaactgggc gaagaaaaac tttggtggcg cttggtggac
    44701 gacagaagcg caattggatt ttatgttcaa aggtgatggt ggatttgttg ctatattaaa
    44761 agattatgct ttaaacgctt ctgatgatgt tgtggcaaat gccgtagcgt ttcataagaa
    44821 ttgggaaatt tcagcagatt ccgaatcagc tgtagtggct gtacgtggtg gaaatgcaaa
    44881 agaaatttgg gaatacatga aaagcaaagg catggacgga gctaaagatg taagcaaaat
    44941 caataaaatt gccggaggaa caagtaccgg tggtggatca aataattcat caagctcagc
    45001 atcaggaaca gtagaagatg cctgtgcaac tacagatgat gctaattctg gttctggatc
    45061 aatgggtaaa gaaggaaaaa ttaacggtaa aaaaggtcaa gtaatagcga atatgactta
    45121 tgaagaagct ttgaaaaaat atgggaaagc gatcacgcta ccaaaattca aagaaccaga
    45181 ttggtcaact tcacctttta acggaatata tcaaggtcag tgtacagagt tgacgtgggc
    45241 ttatatgagc caattatacg atgggaatca accgactact ggaaatggtg gctacgtatg
    45301 ggaaagctac aaatcggcag gagcgaaaat aacgaacaaa ccaatcgtag gttatgggtt
    45361 tagtgcttca gatggatggt tatgggctgt tggcgatgca gggcatactg gtgtagttat
    45421 agcggtgttt gaagatgggt catttctatg tgctaacttt aatgttccac caacccaagc
    45481 tccaacaagg ggagtttcgg taactttgat tgatggagtg gacggatcag ataatgtgca
    45541 tttctttagc ggtgtaggaa aagctaaatt tgaaatcgaa aaataaattg gaatgaagga
    45601 cgatatagat gaatagtaaa gtgaaagata caattatcat ttctttgctt tctggtatgt
    45661 tattagcact gcttgttgga atcctattct tcaataagca gaatcaggac atagaagctg
    45721 aaaaacacag tgtgcatatt gaagataagc tgacattctt taaagaggtt aaaccaaatg
    45781 aggtggatgg attacggaaa gatttagaaa atgatattca agaaatgttg aatggatcag
    45841 ttcaaaataa gtttgcagat caatcaggtg aagaattttt gagaaaacta tttattgtca
    45901 caggtaaacc accaattact gataagagta gtgataaaga aaaaatcgag tactataagc
    45961 cttttacttt taagctaaca aactttggaa ttaagacatc tagtagtggg ttgtatgaag
    46021 cattgaccga ttttgaagtt caatacaatg gaaaaaaagt gtatgatacc acattaacca
    46081 tcacactaaa taatgataag aaagtgactg ggggtgcttt ttatgcaaaa tcatgaaatc
    46141 actactgcta aaaaggcgat tggaatactt tccataattg tgatcctttg ttgtgtggta
    46201 ctatttcaaa agaaaaatgc ttatgataat aatgtcaaag aattggaaga ccttaaaagt
    46261 aaacaaatcg aagtagtcaa gaataaccag aaagtgaaag ctatgcagga agcccaatac
    46321 aaagaattgg ggaatgctga aatcaaggaa caaagtagga aattctttaa tttattttac
    46381 aactggaatt catgggaaaa atatacgaag aatatgaagg aaatacaaaa agaatttcct
    46441 cagatagtaa agtctagtcc tgttgatatt tctggcacga tggtaggcac tggtaatagt
    46501 ccaatatcta gctatactgc tgattactat acaacaaatc aaaaaggtaa aatggttgaa
    46561 ttgattacac agacaagaaa tactccaaca acagcaacaa caacaatttg gcgtggagta
    46621 acaagcatag gagaaaatca attgttttac ttggagaatt tagatagcta tgtaagagcg
    46681 gagtaaaata aaaaaagagc taacggtacc aacgttagct ctttgtgctg acgatgataa
    46741 caaaacctac cgaaaaagga ttgtttcatc acaaacacaa acagaaaaga attgataaaa
    46801 aacccgaacc tcagaatttt tattaatcaa ttcatttaga ttataataaa ttgagctgaa
    46861 cattacaata cgtatatgtt gtaaaacgta aaaaaagtca aaagtaaata ctttcttaaa
    46921 aagtcaaata aaactattaa agacaatata tatggtttat ttgacataag aaaatggtct
    46981 agatgataat tgtaatatag aattgtaggc ttaaataaag cataaaatac cttttatgat
    47041 aaggtatagc tcttaatttt aagcagtttt tagatataag agtgaaagta aagtggggga
    47101 gaataataat ggcagatcgt aaattagaaa agttattaga agaaacgtgg aatccaaaag
    47161 aatttagtga attttttatg gagaattttg aaactgactt agcagtgatt gttaaagatg
    47221 ctcttagaga gcaagggtat cctgaaactg caaactatat aaacattaat tttactttgt
    47281 atactgaaaa taaaggaact tgggattttt gggctacttt agcaaataag gagttaagtg
    47341 ataaatcaga cacaggaatt cgtaactttt ttgaatcaaa cagagatgat tatatgtatg
    47401 ctaaccatca agataaattg aactttagag tagagtttga tgaaacacct gaagaattta
    47461 tagaaagaca accgcctaaa gaaaatgtag caaaggtatt ggaagatcgt tggaattcag
    47521 atgaaatagt ttcaacaatt agcgaactag gcggacaata tgaaccgcta gtagaagcgg
    47581 ttagagaaga gctaagactg aataagtttc ctgatgttca aaatattgat gtttctcaaa
    47641 ttgaaataaa cgtcaagatc acgaataaac ttgattatgg tagttgggca gatatagcat
    47701 tagaaaaata catctactca acgttaaaag agtttatcga aaatagaatg gatatcatgt
    47761 atctacagca tccacagtat ttaaactttg gtgttgaaat agctacacct cttgaagaat
    47821 ggaaaatgga gcaagggctt gactagcctt tgctccattt ttttatttta caaatgaata
    47881 cagttcgtcg tttttaccaa gaatgattgt ttgcccaact ttgagattgt caggtgatcc
    47941 ttcagcggtt tgtaaggaga acgttccgtt gaacttatcg gataccgtta taggtagctt
    48001 taattctgat ccttcctcat ctttgagaat tgctgttttt cgttcatcaa aataaacagt
    48061 ctttattaaa taatttttcg tagtgatctc agcagatttg tctttttttg tagtagtaac
    48121 agtttcttta gaattagagg tattttctac gactattcca tctttggcat ttgagcttgt
    48181 gtttatttga ttgaatattt tcagcgacaa gataccgcta agaatagtca atatagaaac
    48241 aagtgtaatt aatactgata atttcctttt tttgttcatt ttttcttaac ttcctttttg
    48301 ttttgattag ttagatattc cttatctaac aagccttttt aggctattaa atgaggataa
    48361 accccaaaaa aatattgcaa ttatgacagt gcaatggtac tattattata caataaattt
    48421 tgcgaaaggg gtaggtctaa atcgcaaaca aaaagaaatt gaacctagac aaatacaact
    48481 tgtacaccgc aagggaagca agtttgattt tgggtaaagg tagaaattat gttacaaaca
    48541 taatcaacta ttatccaaat cgtttgccta aaggttcgtt caagaaagtg ggtgacaaaa
    48601 cctatattat aacggaagaa ggaatagaag cattgcgatc acaaacagaa aataaaaaca
    48661 aaaagaagga atgaatatga agaaaaattc gcttggtatg gttttatttg catcagctgt
    48721 acttgcgggg ttatcagtac cagtaacctc acatgctgat gaatggactg caaacacacc
    48781 ggatcaaatc caaattacaa caggacaatc aagttatgcg ttcaaaaaag gagatactat
    48841 ttggtcaact gtagagaagc ttaaaagctt aggttacaag actactgttg ctgagtttgc
    48901 cgaattgaac aatgttgatt tggcaaaagg agaacaacgg actatcccaa ttggacgaac
    48961 tttcaacatc agtaaagata cattaacagt taaggaagca gatggcgaag tagttagtca
    49021 aactgttcaa aaagataaac ctgatcctgt tgaaaatacg gagcaagaga acaaaaaatc
    49081 cgagactgca aaagaatcgg aaccaacagc aaacacttct gataagacag aaacaagtgc
    49141 ttcaagtgtt gatggagcaa gttcatctaa tacaaataat tctacaagtg agaaagaagc
    49201 atcacaggga acaagtgaaa gtagcactac ggaaactgat actggaacat caacaaataa
    49261 ctcacaatca acatcaaatg atgcaaatga atcaaccagt acatcttcta gtgatcaatc
    49321 acaaacaggt tcgagtgatg aaactgacag ttctacttca ccaagtaaac ccgaacaacc
    49381 aactaatgtt gtgctaggcg ttccatttat tagtcaaggt aacacaatgc tttgcgaagg
    49441 aacatctttg ctagaagcgt tacattataa aggtgttgca acaaatcaag atttaatgac
    49501 ttttgttaaa tcaatgccac tatctcctga taataatcca aataatggtt tttctggtga
    49561 atggagacac aatgtggatg gaacttatca aggtatgaat ccggctcctg tagtttcatg
    49621 gggtgttgct aatggcggaa atgttgtaga tatttctggt tcatcggtac aaggattaaa
    49681 agatgagatt gtaaatggaa atcctgtagt agcgtgggta acttatgcct ttgaacctgc
    49741 tcaacataaa tcaatgtttt ggggcgatgc tatttggaat cgacatgtag tagccgttga
    49801 tggatttaaa gacggttttt accatgttgt cgatcctgtg tttggggcaa gttggattaa
    49861 tgcaggatca tttgaaacag catataacgt atctcaatta gcggtttcgg tacgttaata
    49921 aaagagattg tttatatact aagtagttaa gtatagtctt tatttgtcct tgcttaacta
    49981 cttagaaaac ttgttgctca tggtgggtag aactaaaagg ggtgatgcta tgaatactag
    50041 agcgttgaaa cagcttgctt tcaacgaaaa aagtgattaa taggtgtggt gattttaact
    50101 taggaatggg cgcgaaggtc attaccacac cgacatatcg tggctcattt tcttcgccta
    50161 actggtataa agaaggagta acgagagatg aagcatgaaa aaacgataat tttaaaatca
    50221 aaaataattt attgttttgc gactttagta atggtgttat ctacgcttat aatgcctttt
    50281 tcagcaataa gagctgaagc agatgtcaca aaaccgacaa gtgcaaaatt tacagaaact
    50341 ggcgtaaaaa cagacggtta tattagggtt aatatgccga atcatccaaa tgaatggatg
    50401 atttcaagtc aatttaaaga ttctcatgga aatattggat attgtatgga ttcagaattg
    50461 ccatccccaa caggaagtgg agccggaagc ttgaagtaca agggagctgg cagtgatgag
    50521 ttctatcgta tgtttaaagg tggttttcca tcaaaaacag caaaggaact gggtgcagga
    50581 aatgatacag aagcatggta tgcaacacaa ttagtttcat gggttttagc cggaaacttt
    50641 aaagtttcac agattgtatg gtcacatcca aaccataccg ccgcagaaac agctcgtgta
    50701 aaaaaagcat ttgaaaaaat ttatgactat gctaagaatg gaaaagatac gcccaacact
    50761 gagttttcta tcacagcttc taagacagct gacgaaggaa aatatcacac tttcacatat
    50821 aaaacagcat caaataagac tggtaatgcg aagctcactt ttacatcagc aaaaccagct
    50881 ggtatgaaga tttatgatgc agatggtaaa gaaattacta acaacactgt gaaattaaat
    50941 tcaagtttta ctattaaagt acctgtgact acaccaagtg gtactttgtc atttaaaggt
    51001 actgctaatg ttagcacaac caatcctttt acttttgatg gacgaggcgt gtatcaagat
    51061 gccgtagtta tgatcactac ttcagaaaca aaggactcta aatctttgag tgctaaatgg
    51121 acaagagctg ttggtcaggt aacagttaag aaagttgatc aagatggaaa tgctttaaaa
    51181 ggagcggagt tcactctaac tgacaataac aatgctaaat ctgttaagac aaccggagat
    51241 gatgggttag ctacgtttga tttgcaacaa ggcttaacat acagcttaga agaaacaaaa
    51301 gttcctgaag gttatcaagg ttcatttaaa aaggatggta ttactgttaa taccgatgga
    51361 caaaaattta gttacactgc tacgaacaca attaaaaaag gtaaagtaaa agttaaaaag
    51421 gttgaccaag atggaaaacc attagctaat gctgaattta ccttgacgga tgattcatcc
    51481 caaacacaag cagaaaaaac agatgaaaac ggacttgcaa gcttcgatat tgtagcaaac
    51541 aagacctaca gccttgaaga gaccggagta ccaactggtt atacaggcgc attcaagcaa
    51601 gaaggaatta ccgttaaaga tgatgg
//
LOCUS       DACCQI010000005        42165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCQI010000005 DACCQI010000000
VERSION     DACCQI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.005"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            109..1173
                     /locus_tag="IVO76_000221"
     CDS             109..1173
                     /locus_tag="IVO76_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7428217.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1419..1844
                     /locus_tag="IVO76_000222"
     CDS             1419..1844
                     /locus_tag="IVO76_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7428218.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1837..2286
                     /gene="nusB"
                     /locus_tag="IVO76_000223"
     CDS             1837..2286
                     /gene="nusB"
                     /locus_tag="IVO76_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7428219.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2383..3237
                     /locus_tag="IVO76_000224"
     CDS             2383..3237
                     /locus_tag="IVO76_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7428220.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3238..4575
                     /locus_tag="IVO76_000225"
     CDS             3238..4575
                     /locus_tag="IVO76_000225"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7428221.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4580..4804
                     /locus_tag="IVO76_000226"
     CDS             4580..4804
                     /locus_tag="IVO76_000226"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7428222.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4806..5696
                     /locus_tag="IVO76_000227"
     CDS             4806..5696
                     /locus_tag="IVO76_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7428223.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5705..6523
                     /locus_tag="IVO76_000228"
     CDS             5705..6523
                     /locus_tag="IVO76_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7428224.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6630..7079
                     /locus_tag="IVO76_000229"
     CDS             6630..7079
                     /locus_tag="IVO76_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7428225.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7097..8779
                     /gene="recN"
                     /locus_tag="IVO76_000230"
     CDS             7097..8779
                     /gene="recN"
                     /locus_tag="IVO76_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7428226.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8834..8950)
                     /locus_tag="IVO76_000231"
     CDS             complement(8834..8950)
                     /locus_tag="IVO76_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7428227.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9023..9979)
                     /locus_tag="IVO76_000232"
     CDS             complement(9023..9979)
                     /locus_tag="IVO76_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7428228.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10150..10518
                     /locus_tag="IVO76_000233"
     CDS             10150..10518
                     /locus_tag="IVO76_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7428229.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10688..11119
                     /gene="mraZ"
                     /locus_tag="IVO76_000234"
     CDS             10688..11119
                     /gene="mraZ"
                     /locus_tag="IVO76_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7428230.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11140..12099
                     /gene="rsmH"
                     /locus_tag="IVO76_000235"
     CDS             11140..12099
                     /gene="rsmH"
                     /locus_tag="IVO76_000235"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7428231.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12104..12505
                     /gene="ftsL"
                     /locus_tag="IVO76_000236"
     CDS             12104..12505
                     /gene="ftsL"
                     /locus_tag="IVO76_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7428232.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12506..14698
                     /locus_tag="IVO76_000237"
     CDS             12506..14698
                     /locus_tag="IVO76_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7428233.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14724..15686
                     /locus_tag="IVO76_000238"
     CDS             14724..15686
                     /locus_tag="IVO76_000238"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7428234.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15700..17061
                     /locus_tag="IVO76_000239"
     CDS             15700..17061
                     /locus_tag="IVO76_000239"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7428235.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17087..18175
                     /gene="murG"
                     /locus_tag="IVO76_000240"
     CDS             17087..18175
                     /gene="murG"
                     /locus_tag="IVO76_000240"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7428236.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18190..19410
                     /locus_tag="IVO76_000241"
     CDS             18190..19410
                     /locus_tag="IVO76_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7428237.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19566..20891
                     /gene="ftsA"
                     /locus_tag="IVO76_000242"
     CDS             19566..20891
                     /gene="ftsA"
                     /locus_tag="IVO76_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7428238.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20914..22155
                     /gene="ftsZ"
                     /locus_tag="IVO76_000243"
     CDS             20914..22155
                     /gene="ftsZ"
                     /locus_tag="IVO76_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7428239.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22172..22849
                     /locus_tag="IVO76_000244"
     CDS             22172..22849
                     /locus_tag="IVO76_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7428240.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22863..23462
                     /locus_tag="IVO76_000245"
     CDS             22863..23462
                     /locus_tag="IVO76_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7428241.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23477..23764
                     /locus_tag="IVO76_000246"
     CDS             23477..23764
                     /locus_tag="IVO76_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7428242.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23813..24595
                     /locus_tag="IVO76_000247"
     CDS             23813..24595
                     /locus_tag="IVO76_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7428243.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24616..25323
                     /locus_tag="IVO76_000248"
     CDS             24616..25323
                     /locus_tag="IVO76_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7428244.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25585..28383
                     /gene="ileS"
                     /locus_tag="IVO76_000249"
     CDS             25585..28383
                     /gene="ileS"
                     /locus_tag="IVO76_000249"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7428245.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28432..29958)
                     /locus_tag="IVO76_000250"
     CDS             complement(28432..29958)
                     /locus_tag="IVO76_000250"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7428246.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(29973..30620)
                     /locus_tag="IVO76_000251"
     CDS             complement(29973..30620)
                     /locus_tag="IVO76_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7428247.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30804..31133)
                     /locus_tag="IVO76_000252"
     CDS             complement(30804..31133)
                     /locus_tag="IVO76_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7428248.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31310..32038
                     /gene="sfsA"
                     /locus_tag="IVO76_000253"
     CDS             31310..32038
                     /gene="sfsA"
                     /locus_tag="IVO76_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7428249.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32054..33067
                     /locus_tag="IVO76_000254"
     CDS             32054..33067
                     /locus_tag="IVO76_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7428250.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33067..34344
                     /locus_tag="IVO76_000255"
     CDS             33067..34344
                     /locus_tag="IVO76_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7428251.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34407..37109
                     /locus_tag="IVO76_000256"
     CDS             34407..37109
                     /locus_tag="IVO76_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7428252.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37261..37578
                     /locus_tag="IVO76_000257"
     CDS             37261..37578
                     /locus_tag="IVO76_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7428253.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37608..37928
                     /locus_tag="IVO76_000258"
     CDS             37608..37928
                     /locus_tag="IVO76_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7428254.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38036..39496
                     /locus_tag="IVO76_000259"
     CDS             38036..39496
                     /locus_tag="IVO76_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7428255.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39564..39785
                     /locus_tag="IVO76_000260"
     CDS             39564..39785
                     /locus_tag="IVO76_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428256.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39816..39998
                     /locus_tag="IVO76_000261"
     CDS             39816..39998
                     /locus_tag="IVO76_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7428257.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            39998..40411
                     /locus_tag="IVO76_000262"
     CDS             39998..40411
                     /locus_tag="IVO76_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7428258.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40534..41715
                     /locus_tag="IVO76_000263"
     CDS             40534..41715
                     /locus_tag="IVO76_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7428259.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
BASE COUNT        14102 a         7158 c         9075 g        11830 t
ORIGIN      
        1 ttatttaaaa taaaaagaag ttttccggaa atttttattt aactgtcgtg tacgaagaaa
       61 agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga gtttcattat gatgctacgt
      121 gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg attcattttt ggttaccagt
      181 ccgtacaatc ttcgttactt aacgaacttt acaggtacga caggattagc tgttattact
      241 ttggaaaaag cattttttat tactgatttt cgttataccg agcaggcggc agcgcaggct
      301 caagggtttg aaattattaa aaatgtcgga cctatttttg atgaggtagc aaacttagta
      361 cgcaaagaag aactgcactc attagctttt gaggaaacaa cagtctcatt tttagaatat
      421 tcggttttag aagaaatcat cgatgcggaa ttagtacctg tttcacagat gatcgaagaa
      481 ctcagagaag tgaaagatga agaagaaatc gctattatcg aaaaagcatg tcatattgcg
      541 gatatggcat atgatcacat cctgaaaatg atccaaccgg gcatgactga gattgaagtc
      601 gcaaatcaac tagactttta tatgcgttct cttggcgctt caggtgtttc ttttgatacg
      661 atcgttgcta gcggattgcg ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc
      721 gaacaaggtg acttgatcac attggacttt ggttgctatt acgaagggta cgtttccgat
      781 atgacacgta cattcgctat tggtgatcct ggggaaaaat taaaagaaat ttatcaaata
      841 gtcttagatg ctcaattagc agttattgat gctgcaaaac caggaatgac tggtgtccaa
      901 ttagatgcag ttgctagaga ttatatttca aaacatggtt acggggaagc atttggacat
      961 tctacaggac atgggatcgg tttagagatc cacgaaggac caaacgtttc ttctcgtgca
     1021 gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac cagggatcta cttaccagga
     1081 atcggtggag tacgtattga ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg
     1141 actcgttcac caaaagaatt gatcatctta tagtttcatc aatagaagaa gtaagcgtaa
     1201 aaatcagtgt tcacagactt tgttatagaa aatcactcga tttaatgata cactatagaa
     1261 gaacttttaa gctgtaagag ataataaagt tgggtgtttt ctccaattca tagcagtttg
     1321 cagctgatcg gcggcagttt tcttaccacg tgatcactat gccgcatcat agagaattcg
     1381 gatgtatctc gtgcttaggc aaagggagga ataaataaat ggcagatgaa aaaaatttga
     1441 ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa
     1501 ttatcgggat tgcagcttct aaagtagaag gcgtatacgg aatgcgcgga acttttgcca
     1561 gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg
     1621 aggaagatgg cttgaaagta gatctttact gttatctaga atatggtgtt tcagttccta
     1681 aagtagcaat ggaaatgcag gaacgggtca gccagcaagt ccttttcatg acggatatcg
     1741 atttatctga agtcaatatc catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa
     1801 caatcgaaga actttttgaa gacgaagagg aagagaatga ataaagaatt atcaagacac
     1861 gagattcggg aaatggcatt gcaggcttta tttccattag acttcaatgc agacctaaca
     1921 aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc gcgacatgat caacgaagat
     1981 gaatcagagt ttgttcctgt ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa
     2041 gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca attggcacat caatcggatc
     2101 tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg aaatgactta tgtgacagat
     2161 gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg caaaaacatt tagtgatgac
     2221 cgttcacgta aattcgtcaa cggtgtttta tccaatgtat tgaaagaatt agaagctggg
     2281 gcgtaagtcc tagctttttt ctatgaatca tcataaacct gttataaatc gggaaaattc
     2341 tatggtagaa tgaaaacaca aaaaatatct gggagtggtt agatggcaga attgattaac
     2401 ggaaaagaat tagctgaaaa aatgcaggca gagattgctg aaaaaatcca aaaattgaaa
     2461 gataatggta tccatcccgg attagtcgtt ttattggtag gggaaaatcc agccagccaa
     2521 atctatgtaa gaaataaaga acgtgtagca aaagaaatcg gaatccattc attggtagag
     2581 cgttatcctg agacgatttc tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa
     2641 gatcctagtt ttcatggtat attggtccaa ttaccattgc caaagcatat agatgaagaa
     2701 aagatcctgt tagctatccg ccctgaaaaa gacgtagacg gttttcatcc gatgaattta
     2761 ggcagactcc ttgcgggtaa accggacaag attccttgta ctccatacgg catcatgaaa
     2821 atgtttgaag cttattctat cgatccggcg ggtaaacgag cagtagttat cggacgaagc
     2881 aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg cagatgcaac tgttactgtt
     2941 gctcattcta aaaccgaaaa tctggctgaa ctggcacaag aagcagatat tttagtcgta
     3001 gcaataggac gtggacattt tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata
     3061 gatgtaggga tgaatcgtga tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa
     3121 gtggagccta ttgcaggctg gatcactcct gtgccaaagg gtgtagggcc aatgactatc
     3181 acgatgctga tgtatcaaac cgtaaaatct gccgaagctt ttggagcagg tgaatagatg
     3241 gcacaagaat atttaactgt cactacatta acgaagtatt tgaaaagaaa atttgatgcc
     3301 gatccttatt tagaacgcgt atatttaaca ggcgaaatat cgaatttccg tttacgtgcc
     3361 aatgcccatc aatatttcag cttaaaagat gaccatgcaa aaatatctgc tattatgttc
     3421 aaaggagcat tccaaaaatt acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt
     3481 gggcgtattt cattatatga agcgggaggt tcttatcaaa tctatgtaga gcacatggag
     3541 cctgatggaa taggtgcttt atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag
     3601 gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc
     3661 cttaccagtc caagcggagc ggttatccgc gatatcatca cgacggtgaa aagaagatat
     3721 ccaattgtcc aattggtttt gttcccaaca gttgtacaag gaaaccaagc agcggacaat
     3781 gttgtcagga atatccagag agtagaagag atgggcaatt tcgatacaat gatcatcggt
     3841 cgtgggggtg gttcgattga ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg
     3901 atttatcaag ctaatacgcc ggtgatttca tctgtaggac atgaaacaga taccacgatc
     3961 gctgatttag tagcagatgt tcgggctgcc actccaacag cagctgcaga attagctgtt
     4021 cctgttttgt cagaagagtt gatgcgcatt caagaacggc aagctagatt gcagcaggcg
     4081 tttgtacgcc agatccagcg caaacaagaa cggttcgagc gtgctcgacg ttcctatgta
     4141 tttcggcagc ctgaaagatt gtatgaaggt cagaccatca aattagacca gttgaaacag
     4201 cgattatatc aagctacaca gcaaatttat catgcgaaag aaaaacaaac gatcgcatta
     4261 agccaccaat tggaacaagt ggctccgctt tatcgagtta aagcagctcg ccaagaaact
     4321 gcttatctag aaaaacgctt agaagagaaa atccaacaat atatgaaaca acagcagcag
     4381 cgttttgctc aagctgttca atctcttgat ttgttgagtc cattgaaaat catgggaaga
     4441 ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa
     4501 ggggatttgc tgacgatcca ttatcaagat ggaatcgtta aaggacaagt acaagagata
     4561 gacaaaggag aataaaaata tggaaaatcc aacttttgaa gagtcactgc aagaattaga
     4621 aaaaattgtc atgcagttag aacaaggaga cgttccatta gaatcagctc tagattcgtt
     4681 taaacgagga atggaattaa gcaaacattg ccaagataca ttagctaaag cagaagagac
     4741 cttgacgaaa atgatgacag aatcaaatga agaagttgat tttgacggaa acgaggaaag
     4801 ctgagatgag attaactgac ttttcagcta ttcatctacc tgctgtagaa aaagaaatct
     4861 tgtctttttt agatgagcat acaacggata gaggactcta tgatgcgatg agttattcag
     4921 tgaaagcagg cgggaaaaga atacgtccct tattactttt aacggccgtt gcttcctttg
     4981 atgagcctat tgatgttccc gtctatcaag tagcagctgc tttagaaatg gtgcatacgt
     5041 attccttgat ccacgatgat ttgcctgcga tggacaatga tgatcttcgt agaggaaaac
     5101 ctaccaatca taaagtattc ggcgaagcat tagctatatt ggccggagat ggtttgctta
     5161 caggagcatt ccagctaatc agtatggcac acttaggtaa ttctcctaag cttctgctgc
     5221 ttcagcaatt agctgtttgt gcgggatcac aaggtatggt tgctgggcaa gctgcagata
     5281 tcgagggcga atcgaaaaag ctttctttag aagaattagc atttattcat gaaagaaaga
     5341 cagggcattt gattcgttat gccttgctgg ctggtggcat attagcgaag caacctgaag
     5401 aaatcttatt gctacttcaa cgattagccg aacatttagg tttagctttt cagataagag
     5461 atgatctact cgatgtgatt ggtacaacga aaacattagg gaaaacagct ggtaaagatg
     5521 aacgtatgga aaaaaatact tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt
     5581 tggagattga gctgttatcc gccaacaaga tcatagacaa attagaagag gacgtttttt
     5641 cttttgatgg cgggctgatg cgccagatga tcaagcaatt tgatgtggag aaatgaggga
     5701 aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa caaggattat ttgaaacaag
     5761 agaacaagcc aaacgttcag tgatggctgg tttgatttac aacgaaaaaa acgagcggtt
     5821 tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta agagtcaaag gtaaaaaact
     5881 tccttatgtt tctcgagggg gattaaaatt agaaaaagca ctcaaagcat ttgatctatc
     5941 tgttgaagga aaaacattgt tagatattgg tgcatccact ggcggattta ctgatgcagc
     6001 tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt ggctacaatc agctagcttg
     6061 gaaaataaga caagatccta gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa
     6121 acccgaagac tttaccttag gaattccaga agttgctgtt atcgatgttt cttttatttc
     6181 actaaaactg atgctgcctc cattacatgc tattttaaaa gaagagggag aagtgattgc
     6241 tttaatcaaa ccccaattcg aagcaggcag agaagctgta gggaaaaacg gaattgttcg
     6301 tgaccctcag acccatcaaa aagttttaga agacattttg tcatttgcgg cacaaggtgg
     6361 atatgatgtg ctggaattaa gttattctcc gatcacgggg ggagaaggaa atatcgagtt
     6421 cttggctcac ttgaggaaag tacccgaaag cggaacaatc aattcagcta ttaatatggc
     6481 agaagtggtt tcaaatgcac acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat
     6541 caagtgagaa gtatgcggat tcttctttat tataccaagg aaataagcta tcattgatta
     6601 agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta
     6661 ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga atttgttgaa atcctgaaaa
     6721 gcaaaggaat atctgttact caagctacga tttccagaga tataaaggaa ctgaaactga
     6781 tcaaggtacc ggcttcagat ggcggctacc gttatagcat accagctgaa acaaacgaag
     6841 atgtaaatac taaattggaa aaactattaa aagacgcttt cgtggcagtt gaccaaatgg
     6901 aaaaatttgt tattttgaaa acattgccgg gaaatgcttc ggcagcagca aatttgattg
     6961 ataaacgtta caagaaagaa ttattttcga ttatcaatga tgacgataat gttttgatga
     7021 ttacacgaac tgaagaccag gcagttttat tgaagaaaga cttcctgcat tacctttaag
     7081 ttgaggagcg aatgtgatgc tacaagaaat cagtattact aactttgcga ttattccgga
     7141 attgcggctt tcttttcacg aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa
     7201 atccattatt atcgatgctt taggtctgtt agcaggagga agaggttcaa gcgattatat
     7261 acgtcaagga gcagaaaaat gtgtgcttga agggttgttt gaactaccaa aacaagaagg
     7321 attctctgaa ctgatggttg aattagggat cgaaacagat gaggacaatc tgattgttcg
     7381 acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt
     7441 agctaatctg agaagaatcg gcagttattt agtagatatc caaggacaaa acgaacatca
     7501 ggaactgctc cagcctgaat cacatcttgc tttattggat cgttttggag atgtagcttt
     7561 ccaacagaag aaaaagagtt atcagcaaga atatatctct tatcgtgagc tggaaaaaag
     7621 ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag agaatagata tgctccattt
     7681 ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga gaagaagaga aattaaaaga
     7741 agaaagagaa aaacttagca attatcaaaa aattgctgat ggattagctg caggttacgg
     7801 agcactgacg aataatgaac aaaacagtgt agatggtgtt ggcttggccg tttcagaact
     7861 gcaagggatc gcccatctag atgtggagta tgaagcaatt tatgaaaata tccaaagtgc
     7921 ttactatttg ctgcaagatg caatcggcga tatgagtcgt cagattgatt tattggagtt
     7981 agatgagagt cgtttagaag aagttacgca acgtttagaa ttaatccgac agttgaaacg
     8041 taaatacggc gaatccattg agtcaatatt ggcttactat gacgaaatca ccgaagagct
     8101 agcttcttct gatttttcag aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa
     8161 agagcttcta cttcagcaac aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa
     8221 agaattggaa aaatccattc tgcatgaatt gaaaagtctg tatatggaaa acactgagtt
     8281 tgaggtacgt ttcttaaaag aagaaaatag acaattgaat catgatggat ttgaccaaat
     8341 cgaattttac attacgacca atccaggaga gccactgaag cctttggtga aagtcgcttc
     8401 tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact attttttctt ctgagcaagg
     8461 tgtgacaagt attatttttg atgaagtgga tacaggtgtg agcggaagag ttgcacaggc
     8521 catcgcagat aaaatttctc aaatatcgaa atactctcaa gttttgtgca tcactcatct
     8581 gcctcaagtt gctgcagtcg ccgactatca atattatatt gtgaaagcag tgattgatgg
     8641 acgtacgcaa acttctgttt cagagttgaa aacaaaagaa agagaagaag aaattgcacg
     8701 tatgctggct ggaagtgaga ttacaaaact cacactcgaa catgcaaaag aactattgaa
     8761 actcgcaaaa aggacctgag ccacctcgca gtggttcagg tccttttcag cagctgatag
     8821 ctatatttta tttttacatg acactcatta cagcagtcaa tacaacagat ccaatcgtta
     8881 aaatcagcat tgtccaaatg acaaattttg ttactttagc aaaagtactt gtttgttttt
     8941 tttcacgcat tgttttcaac ctcgtcatta attagttgat tcaagtgtac aatctttttt
     9001 ccatttccac aagagggctt ttttataaaa acttcccttt ttttaaataa taggtagtta
     9061 cgatacaaat aaataatgtt aatagaacta gccatataaa ggcatcatcc ctttcagcaa
     9121 taggtaactt aatgttcatt ccatagatac tgccgacgat tgtcggtata gttaaaataa
     9181 tcgttaaaga agttaaaatt ttcataatat tattcagatt attcgatacg attgctgaaa
     9241 aagtctgatt catttgttct actagtttta attgaatgcg agaagtagtc attgcctgct
     9301 ctgtttccac caatacatca cgtaaatgat ttttcaaagc atgattttct tggaaaagtt
     9361 tattttgcgc tagttctttc aatgcatcta aattagcaga agtcgcttct ctgaaataaa
     9421 cgaggctttt ttggatatcc atgatttgat aaagttgttt gttttcagtg gatacttgaa
     9481 gtttggcttc caaagtatcg cattgagatt gaagcgtagc taaataactg ttgtaagaga
     9541 gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt aggcgaaata tcactttttg
     9601 gaaaggtctg gctaaagact ttctttaaaa acagtggttc atgattcgta attgtgataa
     9661 gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt atccacttga aaataaccgc
     9721 taggacttgt tttagcaaaa ggatattgta acaataataa cgctggcttt gtcaactttg
     9781 attgattgaa ttcttctgta cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat
     9841 ctgacggaat ttcaaattga tgtgtcagct gatgaatctc ttcttgatta ggacgttcta
     9901 cacaatacca gatcgtttgg tcagtttcct ttccggtatt acgcagtttc tcattatcta
     9961 atgtaaaata tttgatcatg attttcctcc tgttgcctgt ttcttttatt ataacggata
    10021 gggaattgga atacatggaa tgtgtttgta agatatagaa tataactgca taaatttaaa
    10081 taaaatgaaa cagttgtgta aagtatatac agtatacttg aagcaacgat aaaaaaggag
    10141 tttacgttca tgggcacatt cacaccactt atcttgtttt ggtatattta cccggtaatc
    10201 gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata
    10261 aaagcccctg atttggcgat tccttttttg tggggcggta tgcatgcatt gtctagaaat
    10321 acagggacaa tttctatctt accgtttttc tttatttctg ttttgctaat gggaatttgt
    10381 attgctgttt tccaagcata ttattatgaa gaaattattt atccgcgtta tttcaagatg
    10441 acttggcgtt tggtttttct gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac
    10501 atactttctt acctatgaaa aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt
    10561 gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag ttgttcccgg atttcccaga
    10621 aaaggtggta gaaagtgggg gattgtggta gactaatttt agttagtgga ggaatggggg
    10681 gctttcaatg ttgatgggtg aattccaaca taatattgat gcaaaaggac gtttaatcgt
    10741 gccctcaaaa cttcgtgaag aacttggcga aaagtttgtt ctgacacgcg gattggatgg
    10801 ctgtcttttc ggctatccga tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat
    10861 gccgttggct aaaaaagacg cacgtacttt tgtgcgattc ttctattcgg ctgccactga
    10921 atgcgaactt gataaacaag gacgaatcaa tatacccagc acgttaagaa attatgcagc
    10981 tttgacaaaa ggttgcgtag tcatcggtgt atctaaccga attgaaattt gggatgagac
    11041 acgatggcaa gatttttctg cagcagcaga agagaacttt gacgaaattg ctgaaagtat
    11101 gattgatttc ggactataag aataggaaga ggctgactaa tgactgaaac atttcaacac
    11161 tatacagtca tgttgaaaga aacggtagat ggattgaaca tcaaaccgga tggcgtttat
    11221 gtagactgta cactcggcgg tgccggacat agtgaatatt tattaagcca actaagttct
    11281 aaagggcatc tgtatgcgtt tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt
    11341 ttagcttctt atgtcgaaca aggtatggtc acatttatca aagcgaactt cagagaatta
    11401 gaagaacgct tgagtgagca tgtagataag gtagacggca ttttgtatga tctaggggtt
    11461 tcctctcctc agctagatga agcagaacgt ggatttagtt atcatcaaga tgcaccgttg
    11521 gatatgcgca tggatcagtc agctccttta tccgcttatc acgtaattaa tgaatattcc
    11581 taccatgaat tagtaaagat tttcttccgc tatggcgagg agaaattttc caaacaaatt
    11641 gctcgtgaaa tcgaacgagt acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc
    11701 gaaatcatta aacaagtcat cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa
    11761 agaatttttc aagctgtgcg gattgcagta aatgacgaac tcggtgccga agaggcctct
    11821 ttagaacagg caatccggtt attgaaagtc aatggaagaa tcagcgtaat cacattccat
    11881 tcattggaag acagaattgt caaaagtatg tttaaagaat atagtacggt acaagatcta
    11941 ccaccgggac ttcctgtcat acctgacgaa ttccaaccag aactgaaagt cattactcgt
    12001 aaaccaatcc ttccttcaga ggaagagcta acagaaaaca atcgatcacg cagtgcaaag
    12061 ttacgtattg cagaaaaaat aagaatgaaa gaggagtaga atgatggcag aattaaagaa
    12121 aatgcaagat tacgaatatg atttacctga attggtggat cagccggaac aacaaatgcc
    12181 tgattcttct tcgaatcaag aaatcattct tccacaatca ccggcaaaac gattaaagca
    12241 gatttctgct ttggaaaaaa ttgctgtggc aagcgtggcg attgctattg ttgctttatg
    12301 tgttttaaca gttatgctgc gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat
    12361 acaaacagaa atcaatgata aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga
    12421 actttctcgt actgaacgga ttaaacaaat cgcagaagaa aaaggattgt ctattaatga
    12481 cgacaattta aggaaagtga agtaaatgag tcttaaaaat aaaattcggc ggtttatgga
    12541 aaagaaaaat ctaaatccga tgaacaatcg caaaaaagta ggtatcattt tatttgctac
    12601 gagtattgga ttgttctttt tatttgccgt ccgtttctcg tatatcgtta ttggaggtca
    12661 tgttgctggt acctcattag ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt
    12721 aaaagcaaaa agaggaacga tttatgatcg taacggtgtg gcgctcgcag aagatgcctc
    12781 ctcttattca attaaagcga tactctcaaa aacatatacc tctggggata aaaaactgta
    12841 tgttgaagaa aagaattttg ataaaatagc agaaatcctg cataagaatc tttctataga
    12901 caaaaaagat gcactgaata ttttagagga cggcgcaaaa aaagagctgt atcaagttga
    12961 atttggtagc tatgggaaaa atatctccca agaaaccaag caaaacatcg aagcggacat
    13021 gaaaaaagaa ggtgtcgctg gtctttattt tgtcgaccat caggctcgga tgtatccaaa
    13081 tggcgtcttt tcttctcatt ttattggata cgcagttcca gacaaagatg agaatggctt
    13141 agttggtaaa ctaggtttgg agtctgctta caatgatatc ttgagcggta aagatggtaa
    13201 gattatttat caaaaagata attttcaaaa tcctctgcca ggtacagtgg cagaagaaga
    13261 aaaagctgtc gacggacaag atatctacac gactttagac agcagactac aaagttattt
    13321 agaaacactg atggatcaag taaatgaaga atatcagcca gaagaattga cagcagtcct
    13381 gatgaaagca aaaaccggag aaatcttggc aatgggacaa cgacctactt tcaatcctga
    13441 aacaatggaa ggattgactg gaaaagatgc tatctggcgt aatttcttgg tacaagatag
    13501 ttatgaacca ggatcaacca tgaaagtttt cactactgca gctgctatcg aagaaggaga
    13561 attcaacgag aatgaaacct tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa
    13621 tgaccatgac tttggtgaaa aaggtgtgct gaccatgcgt caagctcttt catggtcaag
    13681 taacgtcggg atggtcatac tggaacagcg attaggcgga cgatggtata actatttaca
    13741 aaaactggga tttggacaaa gtactcattc tggattagat gatgaagtaa acggtgcttt
    13801 acctacatta aatattgtcg accgagcaat gagtgcttat ggacaagcgg taggtgtgac
    13861 gaatttccaa atgatgaaag ctttcacttc tattgcgaat aacggtacaa tgattcaacc
    13921 tcgatatatc agcaaagtgg tggatcccca aactggagaa gagcgaacaa ctcaaacaga
    13981 agttctaggg caaccttttt caaaagaaac aacagaaaaa gtccgtgaat atatgagaga
    14041 tgttgtcgaa agcgaaaact acggtattgc ttatggtgta tatagtgtac ctggatacaa
    14101 tgtatcggca aaaaccggaa cagcccaaat cgcttctgat acagggggat atcaaacagg
    14161 agatacagcc tacctgtatt cgattgtgga gatggttcct tctgaagatc cagactatgt
    14221 cttgtatcta acaatgaaac accctaagac atatgatcga atggcattgg ctaaaatcgc
    14281 aaatccattg atgaaacggg caatggattt taaagaaact gaagaagatt cagatacaga
    14341 aacaaaaaca gaaaaagtat ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc
    14401 agctgatgct caaaaaagtg gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca
    14461 aaaacaatct actgccaacg gtgatcagtt gatctctgga gaaaaactga tcctatatac
    14521 tggtggtgat aagctgatgc ctgacgtaac aggatggtca aaagcagata tcatgaagct
    14581 ggggaaaatc ctcggtattg aagttagttt tgatggagat ggttattgtg tcaaacaaga
    14641 acttgctcca tatgaaaaaa tcactgaaga caaacttagt tttactttag aagaataaga
    14701 agagaaatag gagagaaaag agcatggaat ggacacaagc attgattccc attgtcagca
    14761 gttgtgcaat gacaattgcc gcaatgccgc tatttattgg ttattttcaa atgaaaaagc
    14821 aaggtcaggc gattcgtgag gagggaccaa agtggcacaa tagcaaagcc ggaactccta
    14881 caatgggcgg cttagtgttt ctgatcggct ctattttgac aggaatttgg gttggagctt
    14941 ggcaaaaaca gctaactcct actcttttca tcttgctttt tgtattagcg ttatacggag
    15001 taatcggctt tcttgatgat tttataaaaa tattcaagaa aagaaacatg ggactgaact
    15061 cgaagcagaa attgattgga cagattctag ggggaatcat cttttatctt gtttaccgtt
    15121 ctgaaggtta tccaggtgta ttgaatttct tcggactaga tctaccttta ggaatcattt
    15181 atggtatttt cgctctcttc tggcttgttg gattttcaaa tgcagtaaat ctgacagatg
    15241 gcatcgatgg attagttgct ggcttaggaa gtatctcttt tgcagcttac ggtctaattg
    15301 cttggcatca gcatcagtat gatgtattaa tcatttgctt aagtgtttta ggaggtttac
    15361 tgggtttctt cgtctacaac agaaaacctg ccaagatttt tatgggagat gtcggttcat
    15421 tagcgttggg cggactttta gcggcaattt ctataatgct aaaccaagaa tggacattgc
    15481 ttttgattgg tttgatctat gttatggaaa cagcaagtgt gatgcttcaa gttacttcct
    15541 ttaagcttac aggaaaacga atttttaaaa tgtctccaat tcatcatcac tttgaaatgt
    15601 gcggatggtc ggagtggaag attgatacag ttttttggtt gatcagcatt gtgacttcgt
    15661 tgatcacatt atggatcgtt tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat
    15721 gaaaataaga aagtgcttgt cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta
    15781 ttacatgaat tgggcgcact tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg
    15841 gaagcacaag agctgctttc tttagggata aaagtaataa caggtagtca tccaatcgaa
    15901 ttgttggatg aagaattttc attgatggtc aaaaatccag ggattcccta cagtcatcct
    15961 ttagtagcaa aagcccaaga aatgggtatt ccggtaatta cggaagttga gctagcttac
    16021 gaagtagcgg aatgcccaat tattgggatt acaggaacga acggaaaaac aacgacaaca
    16081 acgatgacag gactactact gaatgcagga gcagatcaag gaattgcacg tttagcgggt
    16141 aatattgggt atccagcaag tggtgtagcg caagaagcaa aatcagaaga taaaattgtg
    16201 atggaattgt ctagcttcca actcatggga atcacagatt tccgccctca tatagcggtg
    16261 attaccaata tctatgaagc acatattgat tatcatggta caagaaaaga atatgtcaaa
    16321 gctaaatgga accttcaaaa aaacatgaca gaaaaagatt atctgatcct taactggaat
    16381 cagtcagaac tacaagaatt agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca
    16441 aaagaagtgt tggaagacgg tgtgtatgct gacgattact caatatacta taaaaaagaa
    16501 aaaataatgg aaatatctga acttggcgta ccaggtaagc ataatgtaga aaatgcacta
    16561 gctgctattt ctgtagcaaa attgtatggt atatcaaatg aagctatacg agaaacgttg
    16621 cattttttcc atggcgtacc acatcgtacg caatatgtag gagaaatcca aggcagaaaa
    16681 ttttataatg attcaaaagc aaccaatatt ttagcgacta aaatggctct aagtggattt
    16741 gaaacgtcta aggtcgtgtt attggctggc ggattagaca gaggaaatac atttgatgaa
    16801 ttaattcctt cattaaaagg aatcaaagca atggtcgtat ttggccaaac aaaagaaaag
    16861 ttgatggatg ctggaaaaaa agcaggcatc gagacaatag taacagccga ttctgtagaa
    16921 caagcagttc ctttagcatt agaaaacagt acagatggtg atgtagtttt attatcacca
    16981 gcgaatgcca gctgggatca atacccaaat tttgaaacta gaggaaacag attcatggaa
    17041 gctgtgaacc gattaaaata gaaaaagtag aagcgagcga taaaaaatga aaattttggt
    17101 tacaggcgga ggaacagggg gacatattta tccagctctt gcctttgtca attatgtaaa
    17161 gactcaagaa ccgaatgctg aattcatgta tgtgggagca aaacgaggtc tagaaaacaa
    17221 aatcgtccct gatacaggaa tgcctttcca taccttggag attcaaggat ttaaacgaaa
    17281 aatttccatg cataacatca agacggtccg attgtttctt aagagtatcc gtcaagcaaa
    17341 aaaaatcctc agtgattttc aaccagatat cgttatcggt actggaggat atgtttccgg
    17401 agcggtagtg tacgctgcag ccaaaatggg tattccaaca gttatccatg aacaaaacag
    17461 tgtaccaggt gttaccaata aatttttaag ccgatatgtg gatcggatcg ctctttcatt
    17521 taaagatgcg gcttcctttt ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc
    17581 acaagaagta gcagatacta aaaaatcaga tattttgaaa acatttgatt tggatccaga
    17641 aaagaaaaca gtcttgattt ttggcggtag tcaaggagca cttaaaatca atcaagcagt
    17701 atcagttttt ttaagtgaat atattccttc ttcttaccaa gttttgtatg cttcaggaga
    17761 tagatattat gaagagatcc taaataaaat aaatcctgaa atacaaaata ttagtgtcca
    17821 gccttatatc aaaaatatgg cagaggtcat ggcttccagt gatctgctgg tggggcgtgc
    17881 tggtgcaact tcgatcgcag aattcacagc tctggggtta ccggcagtgt tggttcccag
    17941 cccatatgtt acgaatgatc atcagactaa aaatgcaatg agtcttgttc acgcaggagc
    18001 agcaaaaatg attgcagata atgaactaac aggtgaatca ctaagtcaga cagtcaacga
    18061 aatcatgggt gatgaagaat tgcaaaagca aatgtgccga gcttctaaag aacaaggaat
    18121 ccctgatgct tcaaaacgtc tttatgactt agtaaaacaa ataatcaaaa aataaaggcg
    18181 gtggtcttag tgagtaagaa aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa
    18241 aacaatttga ctccttggca aaaggctaac cgaaaatatc tggcagaaca agagatgaaa
    18301 aaccaagagc caaaaaaaga agacataaca ttagatacac ccctttcaga ttctgaagaa
    18361 gaaaaaacgg ttcaagagga acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt
    18421 tttgaagaga tcaagcaaaa gaaaaacggt ggaccatata acggttcttt tttgaaccgg
    18481 ctgcccaatt taaaaagtca acggcacaaa gtactatttc gtcggttagc tttgatcatc
    18541 actgtattgg gtataccgct gatttttttg atctattatg tttctcctta tagcaaatta
    18601 caagcagttg cagtatcagg caataagaca gtcaacagcc aagaaatcat ctctgataca
    18661 aaattgtcat taggagaaaa tgtttgggga cagtattttc atcgatctac ttatatcgat
    18721 cgccttaaaa aagcacagcc aagaatcgaa acagcaaata tacatttcaa agggatgaat
    18781 gaattcgatt tggatgtaac agaatataaa gagattgctt tgatcgcaaa aaacaatcaa
    18841 tactacccag tgattgaaaa tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa
    18901 aatctcccta tcttggaaga gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa
    18961 tacaatcagt tatcatcaga attacaaaaa gcaatttctg aaatcaagta cacgccaaga
    19021 gcaagcaata aaaatctgat ccaattgaat atgaatgacg gcaatcaagt tattgtaaat
    19081 atcaataatc ttgcgaacca aatgaaatat tattcgcaag tagccaaaga tatggatgaa
    19141 aaaggtgtga tcgacatgga agttggtatc ttctcgcatc cttacggtag cactcaagaa
    19201 acggaagaat cgggccaatc ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca
    19261 aacgaatcaa gtgaagaggc agaaacgcaa aaagcacaaa cacctggatc gtctagcaca
    19321 aacggatcat ctgattcaaa tgattcaagt gaaacaagtc aaatagaaga taatccaagt
    19381 acaactccgt caagtttaaa tgaaaattga taaaacaact ttaaaaagta agaatgtact
    19441 ttctaaaaac cagtaattta tggtagaatt ggaagcagtg aatatatcta ttaataagta
    19501 tattttaaaa cggacgctag ttgttcgctt gtatatggaa tagtaagtag gagggaatcc
    19561 cattcatggc aaaaacagga atgtatgtag gccttgatat tggaacgaca tctgttaaag
    19621 ttgtcgtggc tgaatatatc gacagccaaa tgaatattat tggagtagga aacgcaaaat
    19681 cagaagggat taaccgaggc attatcgttg atatcgataa aacggtccaa gcgatacaac
    19741 gagcagtacg acaagcagaa gaaaaagcag gtatccagat caaaggcgta agtgtcggtt
    19801 tgccagcaaa catgcttgaa gtagaaaatt gccagggaat gattgcagtt aacggagatt
    19861 caaaggaaat caccgatgaa gatgtcagaa atgtggcttc agctgccctt gttcgttcaa
    19921 tacctcctga acgtcaaatc gtgtcaatat tgccacaaga ttttactgtc gatgggttcg
    19981 agggaatcaa agatcctcgt ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt
    20041 ttacaggacc taaaacgatc atccataaca tacgtaaatg cgtggaaaat gccggattaa
    20101 tcgtcaacga aatggtgatt actcctttag cgctgactga atcaattctt tctgacggag
    20161 aaaaagattt tggtacgatc gtcattgata tgggtggcgg ccaaacaaca acggctgtta
    20221 tgcatgataa gcaattgaag tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta
    20281 aagatatctc tattgtatta aatacgtcat ttaacaatgc agaagcgttg aaaataaact
    20341 acggggatgc atacccagaa cggacatcac cagatgaaga atttccagta gatgtgattg
    20401 gtcagtcaga accagtgaaa gtcgatgaac gctacttgtc agaaattatt tcagctcgaa
    20461 tggaacagat tttcaacaaa gcaaaagatg cattggacca aatcgaagca ttagagttgc
    20521 caggtggcgt cgtattgaca ggcggagctg ctagtcttcc tggtgttgtg gacttggcac
    20581 aagaaatctt cggcgtaaat gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc
    20641 cggtatttac aaatgtaata agtatcgtgg attattcagc aaacttgagt gaagtgtatc
    20701 aattggcaaa aattgctgtt acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac
    20761 aagaaacaac aaacacatat gagagctatg aagcacctga agaaacaatc tacgatgagc
    20821 cggaaccaaa agagtccgga gaaaatgtaa aaaataaaat caagggtttc tttacaaata
    20881 tttttgatta attcatcctg ggaggaataa gatatggaat tctcaattga taataatatc
    20941 aatgacggcg cagtaatcaa agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac
    21001 cgaatgattg aagaaaacgt taaaggtgtt gaattcatta cagccaacac agacgtgcaa
    21061 gcattaaaaa attcaaaagc tgaaacagtt atccaattag gccccaaata tactcgtggt
    21121 ttaggtgctg gttctcagcc agaagttgga caaaaagcgg cagaagaaag tgaacaatcc
    21181 ttacgcgaag ctttagacgg tgcagatatg attttcatca ctgcaggaat gggtggagga
    21241 accggtacag gtgctgcacc aatcgttgca ggtatcgcga aagaattggg cgcattgact
    21301 gttggtgtcg tgacacgtcc attcactttt gaaggaccga aacgtggccg ttttgctgca
    21361 gaaggtatcg ctcgtttgaa agaaaatgtg gatacattat taattatctc aaataaccgt
    21421 ttattagaag ttgtagataa aaagacaccg atgcttgaag ctttccgtga agcagataat
    21481 gttttacgac aaggtgtaca aggtatctct gatttgatca cagcgccagg ttacgttaac
    21541 cttgactttg ctgacgtgaa aacagtgatg gaaaaccaag gtacagcatt gatggggatc
    21601 ggtgtggcta gtggagaaga tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca
    21661 ttgcttgaaa catctattga tggagctgaa caagtacttc ttaacatcac aggcggtttg
    21721 gatatgacat tgttcgaagc acaagatgct tcagatatcg tagcaaatgc agcaactggc
    21781 gatgtaaata tcattctagg tacatctatc aatgaagaaa tgggcgatga aatccgtgtg
    21841 acggtcatcg caacaggtat cgatgaatca aaaaaggaac gcaagtctag ccgaccagct
    21901 agacaagcac aaatgcaatc acctgctcaa aagactgtat tagatatgga tcaagctaaa
    21961 ccaacttctt cagaagaaga aaacagtttt ggcgattggg atatcagacg tgaacaaaat
    22021 gttcgtccta gagttgatga ttccaatttt gacaatatcg aaaaaaaaga atttgataca
    22081 ttcaaccgcg aagaaacaaa atctcatggc gatgatgaat taaatacgcc accgtttttc
    22141 cgtcgtaaaa aataaaggag aggaagagca catgattgct gacaacttgc agaaaatcaa
    22201 gcaagaaatt caggattcgt gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt
    22261 agctgtaaca aaatctgttg aatccgctca agctgaagaa ttggttagag ctggcgttat
    22321 gaattgtgca gaaaaccgag tagataaatt attagaaaag aaacaagatt tatttgattt
    22381 tcctgaaata aagtggcatt taattggtaa tttacagcgc agaaaggtaa aattgattgt
    22441 aaatgaaatt gattactttc atgcgttgga cagtttaaga ttagcagaag aaatccagaa
    22501 gcggttagag aaagaattat cctgttttat tgaagtaaat gtttctggtg aggaatcaaa
    22561 acatggaatt tcaccagatg agcttttacc gtttgtggaa tctttagctg cctgcacaaa
    22621 aatcaaggtt gttgggttga tgactatggc gcctaaagaa gcttctgatg agcaaatcca
    22681 taaagtgttt ggtacgctga aagaattgcg tgatcttgtt caagcaaaag gttatgccca
    22741 tgcaccatgt agtgaattaa gtatggggat gagtcaagac tttcaaattg cgattgaaga
    22801 aggtgcaaca tttatccgtg taggaacagc gctattcaaa gacgaataaa ggaggaacaa
    22861 aaatgccgct tttaaacaga ggagcaattg ctaatttttt cggattagct gatgaggaag
    22921 agtacgaata ccaagattat cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa
    22981 atacaacaca gaatcaatca actcattctg tatctgctgc gaatactcat gaaaaacaaa
    23041 aaacggcgac ttcacgtcct gtaacgcatg aaagtattca gccggctgaa aagaaaaaag
    23101 taattgaact acatcaatca cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa
    23161 cctctacaca acctaaagca aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt
    23221 attctgaagc tatggcgatt gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt
    23281 ttcatttagt agaagaaaaa caagcgagaa gaatcgttga ttttctaaca ggaacagttt
    23341 atgcactaga tggagatatc aaacgtgttg gagatgagat ttttctttgt acaccgactg
    23401 ggatggagat tgacagttca accgctcaat cttttgcaga tgcgaatttg tttgatctgt
    23461 aagaggagga actacgattt atactattgc actattactt catttacttc aacgggcagc
    23521 ctatttctat agcattatct tgatggtata tgcattgctc tcttggtttc caggcgggta
    23581 tcaatcaggg ctaggacgat tattagctaa gatctgcgaa ccgtatatca gtttgttcga
    23641 tcgtctgcct ttgaggattg gacccttgga tttcacaatt ttaggggcaa tcctgatctt
    23701 gaacttagcc gtacaaggtt tagcacagat cgttatatgg ataactgtcc atttagttta
    23761 ttgattgaag caacaaaatc tttattctca gaatagatag gaggggaaaa ttttggatgc
    23821 aaatgtatac cagcactttc gggcagatga acgtacattt atcgattcgg taggcgattg
    23881 gattgaacag gttcaaggac aatatgcacc ctatctgacc gaatttcttg acccaagaca
    23941 atcctatatt ttagaaacac tgattcgaca aagcagtgat ttgcgtttca tgttttatgg
    24001 cggatatgaa caggcagaaa gaaaaagatg tttgattttc cccgattatt acgaacctgt
    24061 agaggaagat tttgaaacag cattgtatga aatccattat cctagcaaat ttgccagcct
    24121 aagtcatggg aaaatccttg gaactttagt gggaacgggg atcaaacgct cttactttgg
    24181 cgatatcatc tcggacggtg atcgctggca agtatttatt gcaaatgagg tggatcattt
    24241 tgttgctgca caagtaacaa agattggaaa ggttgctgta cgcttggaac caatcaaata
    24301 cacagaaatg attatgccga aagatagctg gacacaagaa agaggaacag ttagttccat
    24361 gcgactagac agtgtgatct ctgagattta taatatttcc agacagcggt ccaagcagct
    24421 gatcgaagct ggtaaagtaa aagtgaactg gacagaaaat acaagacctg attttgtact
    24481 tgaactatta gatattattt cagtcagagg ttatggcaga gtccagatcc aagaacttga
    24541 ggggaagacg aaaaaagaaa agtacagggt actgttcggt gtattacgta aataaaataa
    24601 aaaggtggtt tactcatggc tttaactcca ttagatatcc aaaataagac attcccgaca
    24661 aaaatgcggg gatataatca agacgaagta gatgatttct tagatttagt tgttcgtgac
    24721 tatgaggaat tgactcaaag aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa
    24781 aaacttgagt acttcaatga attaaaagat gctttgaacc aatcaatcat cgtagctcaa
    24841 gatacggctg ataaagtaaa aacaagtgca agcaaggaat ccgaagtgat cgttacttct
    24901 gcacaaaaca aagcagatga gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca
    24961 actgatgcag aagaaaaagc aagaaaaatc ttgacagatg caacagaaaa agcaagacag
    25021 cttgcgacag aaactgaaga tcttaagaaa aaaacacgag tattccatca acgtatcagc
    25081 ttgatgcttg agtctcaatt agaacaagtg aaaagtccgg aatgggatga aattttacaa
    25141 ccattctcca gctacgtgac tgacagccat gaagtgatca aagaagtttt agcaaaacag
    25201 cttgacaatg aaaatgatac tgatgtaaac tcagacacag gagtcataga agcacctatg
    25261 acggcatttg atacacaggc tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag
    25321 tagaacagaa aagcactttg ttattcttaa agcgagccga tgagagtgga agttcggtaa
    25381 gacactgcat ggtgaaagat cctctacttg agacctgctt ttccgaaatt agagtaagaa
    25441 gcagcggctg acctcgttat tggtcacaga gagtttgcac tagatagatg caactaaatt
    25501 tgggtggtac cacggcatgt tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa
    25561 acaaataaag gagtgtatgg ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca
    25621 aaattcccta tgcgtggtaa tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa
    25681 gaaaatcgaa tctaccaaaa aagacaagag ttgaacgaag gaaaacctag ctttgttctt
    25741 catgatggac ctccatatgc caatggtaat atccatttag gacattcatt gaataaaatc
    25801 agtaaagaca ttatcattcg ttcaaaatca atgtctggct atcgtgcacc atatgttcca
    25861 ggatgggata cacatggatt gccgattgaa caagtattgg ctaataaagg cgtgaaacgt
    25921 aaagaaatgt ccatggcaga atacttggaa aaatgtcgtg aatatgcact ttcccaagta
    25981 gataagcaac gagcagattt caaacgccta ggagttgcag gagattggga acatccttat
    26041 gttacattag atccttctta tgaagctgca caagtacgcg tatttggtaa aatggctgaa
    26101 aaaagctata tttacaaagg attgaaacca atttactggt ctccgtcaag tgagtcttct
    26161 ttagcggaag ctgaaattga atataaagat gtaaaatctc catctatttt tgtagctttc
    26221 aatgtcaaag acggcaaaga tattcttgat gaagatacag cttttgtcat ttggacgaca
    26281 acaccatgga cattgccagc aaaccaaggt attgctgtaa atccaaaatt tacgtatgta
    26341 ttggtggaag ccgatggccg taagtttgtc gtagcgaaag atttgctaga aacagtccaa
    26401 tcagaaatcg gttgggaaaa tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa
    26461 tatatgactg caagtcatcc attctatgaa cgtgaatcac ttgtgatttt aggcgatcat
    26521 gttactttag acgctggtac tggattagtc catactgctc caggacatgg ggaagatgac
    26581 tatatcgctg gtagcaaata taaactatct gtagtgtcac ctgtagactc aaaaggattg
    26641 tttacagaag aagcacctgg atttgaaggt atcttttatg acaaagcaaa tcctatgatc
    26701 actgatctat taaaagaaaa aggagctttg ctgaagctag atttctttac tcatagttat
    26761 cctcacgatt ggcggacgaa aaagcctgtg atctatcggg caactccaca atggtttgca
    26821 tctatcgata aattccgtca gaatattttg gatgaagtag aaaaagtaga gtgggttatc
    26881 ccatggggta aaacacgttt gtataatatg atccgtgacc gcggcgattg ggtaatttcc
    26941 cgtcagcgtg catggggagt accacttccg attttctatg ctgaaaatgg agaagcaatc
    27001 atcacaccag aaacaatcga acatgtggca aaattatttg ctgaacatgg atcaattatc
    27061 tggtttgaaa aagaagcgaa agaacttctt cctgaaggat ttacccaccc aggttctcct
    27121 aatggcgaat ttacaaaaga aaaagatatc atggatgtct ggtttgattc tggttcttct
    27181 catgaagctg ttttacgtca gcgaccagaa ttatcattcc cagcagatat gtatttagaa
    27241 ggatcagacc aatatcgtgg ctggttcaac tcaagtatca cgactagtgt ggctatcaat
    27301 ggtgtagcac catacaaatc agttatttct caaggatttg ctttagatgg tgaaggccgt
    27361 aagatgagta aatctttagg aaacatcatt gcaccagaaa aagtgatcaa acaatttggt
    27421 gcagatatcc ttcgcttatg ggtatctagt gtagattatg aagcagatgt tcgtgtttct
    27481 atggatatct taagtcaagt ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc
    27541 ttattagcga atacagaaga tttcgatcct aaaaaagata ctgtttcgta caatgattta
    27601 cgttctgtag acaaatatat gaccgttcga ttgaaccaag taatcaaaga aatccgagaa
    27661 gaagggtacg acaaatataa cttcatgcat atttaccgta cagttatgaa cttcttgacg
    27721 gttgatctat cgtcattcta cctggatttt gctaaagatg tagtttatat tgaagcagaa
    27781 gatgattatc aacgtcgttg catgcaaact gttttctatc aaacagctgt agctttgaca
    27841 aaattattga ctcctatcat tccgcataca gcagaagaaa tctggtcatt cctgaaagaa
    27901 gaagaggagt acgttcagtt aagcgagttt cctggttatg aagagtttgc aaaccaagaa
    27961 gaattgatgg atacctggac tgctttcatg gacttccgtg ataatgtctt aaaagcattg
    28021 gaagaagcaa gaaacagcaa attgattggt aaatctttgg aagcaaaact aactgtttat
    28081 ccaaaacaac aagttcgtga aatgttaaaa gctttggatg cagatattgc acaattattg
    28141 atcgtttcac cagactactt tgaagttatg gcagcagatg aacaagtgcc agataacgct
    28201 atggtatttg atgatgttgc tattacagtg gaaaaagcag acggggaaac ttgcgatcgc
    28261 tgtcgccaag ttcgtaaaga tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt
    28321 tgtgcaaaaa tcgttgaaga cttctatcct gaagctgtag ctgaaggatt cgaagaaaag
    28381 taaaagggaa aagggggtgt gacatgttct ttgtcacacc cccttttgtt atcaatcgtt
    28441 taattttcca cgttcacgat accagttatc tggttgccag atccattcga atccgtcttt
    28501 ttcaagtagt tcgaaagctg ctttaggtcc cattgatcca gctttgtagt tgggaaaatc
    28561 cggttgtgtt tggtcccaag cttttcgaat gacatccacg atatgccaag attgagcgac
    28621 ttcttcccaa tgagagaagt tcgttccatc accattcaac gcatctaata gtaatttttc
    28681 gtaagcttct gggctatttt caaccatttc tgcactattt ctaaattcca atttgattgg
    28741 ttccgtttca aatccttgcc ctacttcttt cccattcaag gataaagaaa atccttcagt
    28801 tggctgaata tagatcgtta gaacattagg tggaagtgtc gtatcgtcac atggttcatc
    28861 gacagatgtt ttgaatacat taacaggaac ttgtttgaat acgatgttga tccgagtccc
    28921 tttttcagtc aaacgtttgc ctgtacgtac atagaaagga acacctgacc aacggaagtt
    28981 atcgatagtg aatttgcctg cgacgaatgt ttcagtctct gattgagcat ccactttgtc
    29041 ttcttcgcga tagcccttga attgtacacc atccaattca ccttgagcat attgcccgcg
    29101 aacgaagttt tctaacactt cttcttcgtt ataaatgcgg atagctttca atgctttgat
    29161 cttttctgta cggatttctt tttcggaaaa tgctactggc ggttccattg caagtaatgc
    29221 tacaacctgt aaaatatgat tttgcaccat gtctttaagt gcgccactat gatcgtagta
    29281 gcccccgcga tcttctacac cgaggttttc gccaaaagta atttggatat tgtcgatata
    29341 gcgattgttc cattgagcct caaaaatatt attggcaaaa cggatagctg agatgttttg
    29401 gatcatttct tttcctaaat aatgatcaat cctgaaaata tcttgttctg ggaatacttc
    29461 tctgatttgc tggttcaatt caaaagcaga ctggtaatca gaaccgaacg gtttttctat
    29521 gatcaaacga ttgaagccat tttttgtcat aatattttgt gatttcaaat gagaaacgat
    29581 cgttccaaaa aattgaggtg ccatagccaa ataatagaca cggttaccgt ttaattgata
    29641 gtgtccatct aatcggtcag acaattcttt taatgtctga taatgcgttg aatcatttac
    29701 atcatgggat tgatagtaaa aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt
    29761 caatgcacgg attgtttctt tgacaacttc acggtagtgc tcatcgctcc agggacggcg
    29821 agcggttccg attacagcaa aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa
    29881 agacgggtac aatttacgct gggctaggtc acctgtaccg ccgaatatcg taaacaatac
    29941 atttttttca ttagacatgt ggatacactc ctttatattt tatcgacaaa aatcgtgctg
    30001 gccgccttaa agctgacggg gattttcttt ctatcatttt ggatagtgat cggtccttca
    30061 taagctgcga tgtcaacgat ctcatattct tcgttgatat tcaggtcaat agtgactaag
    30121 taatccaaca attctttttc atcaagcact cgtgcgatac gcacttttgt accgacagga
    30181 taggactcca atgtttgtcg gcgttcttca tggacaactt cctctttttt aggaatcgat
    30241 cctccatgag gacaatgttc aggatgattc aaatagtttt ctaaatgatc tgcaagatgt
    30301 tccgaagtca catgttccaa tacttcagca tcatcatgga cttcattcca gctatagtct
    30361 aaatgttcaa ctaaaaaaac ttcccataag cgatgtttgc gaatcagact gctagctttt
    30421 tgcagtccgc ttgcagtcaa ttgtacacct tgataagggg aatgttctac aagctcttct
    30481 ttcacaagtt tagaaatcat ttcactgaca gatgcagctg agacatctag actagatacg
    30541 atttgtttat tgttgacttt cgttgtatct ccacctagtt ctaaaatgat tttcaaataa
    30601 tcttctcgat ttggtgtcat attcgatcat cctttcatgg taaaaaacca tcattagtaa
    30661 gtgtaacaaa aaaaacgtta attgactatt tttctaaata aaaaacaaag gatttttaga
    30721 cgtttttaca acaaaaaacg aaagatgata gagaagactc attctttcgt ttataaagga
    30781 tatatatata cattgggagg gatctatttt tcagataaca acttatatga agtgtctgca
    30841 gattcgtcat aaagagtttg ttcgggataa gaaaagataa gataaaaacc atactgatca
    30901 cgtacgacta aacgatattc atctatttct accacagatg tgccaagaga ctgaccgttg
    30961 atcaaaaacg tgtaatcaga tgtaatctgt aattgttgtt tttgctggct atcactaaag
    31021 aaccatgtac cttgatactt tgagagtcct tctgactttt ccgcttgttt tttctttttc
    31081 aagtaaataa aacttgagag aacggtaatg agtgaaccaa gaaaatagat catttttttt
    31141 atcaccaaat cactccttta tgagaaaata atcgcataga catattcttt cttctttatt
    31201 tcattgtacc gaataaaagc taaaatttct ctaagacttt cgactggttt ttttactgtt
    31261 tttctacaaa ttatgatagg atacatagta ggaagaaagg tgacataatt tgaagtatca
    31321 gcaagtggaa atagcttttt ttattgaccg cccgaatcgg tttatagcac attgtatgaa
    31381 aaaagacgga gaaataatta ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct
    31441 gataccaggc gctgaagtgg ctattgtatt tgctcctgga cctaaacgga aaaccgccta
    31501 tgatctgatt gctgtaaaaa agaagggtga ctggtttaat atcgatagtc aattgcctaa
    31561 tcggctggca attgatggta ttttagacgg cacgatcctt ttaccaggat taactggtcc
    31621 atttacggta tacaagcgtg aagtaactta tcttcactcc aaatttgata tttatttaga
    31681 gacggtggtc ggacaaaaag catttgttga agtcaaaggt atgacacttg aaaataaagc
    31741 gattggtgct tttcctgatg cgcccacact cagaggattg aaacatatag gagaattaac
    31801 gtatgccgca caagatggtt atgccgctta tgtcttgttt attgctcagt tcgaacatct
    31861 tcatctggca acgatccatg aagaaatgca accagcacta gctgatatgg ttcgacatgc
    31921 gcagcaaagc ggagttcaaa tcctcgccta taattgtcaa gtatcggcag atgaggtaac
    31981 tgtcaagcag gccattccat ttgatgtaga tatccctttt gaagatccta tacaatagaa
    32041 agaaggtaaa ataatgatca acttaggtat tatcggaaca aattggatta ctcatcaatt
    32101 tgtccaagca gctttaagca caaaaaaata tgaattgaca gcggtatatt ctagacattt
    32161 ggaaaaagca caggaatttg gttctcatta tgaaggggat atcgaattcg caacagattt
    32221 gaagactttt ttcaatatta ctcatatgga caccatttat attgcttctc cgaatagtct
    32281 tcactttagt caagctaagc aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc
    32341 agctttttcg acgccaaaag aaatggatga aatcatccat ttggcaaatg aacacaatgt
    32401 gtattttttt gaagcagcaa gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta
    32461 cttaccatta aaagacgaga taatcggtgc aaatttcagc tatatgaaat attcttcgcg
    32521 ctatgaccaa gtattagatg gagaagaacc gaatatcttc tcgactcatt tctcaggtgg
    32581 tgcattgatg gatttaggtg tttaccttgt atatgcagct gttgcttggt ttggaatgcc
    32641 aaaagaagtc cattatttcc caagaaagat ttctactggt gtagatggtc ttggatgggg
    32701 gatcttacgt tatgagaatt ttgatgtatc gatccagcca ggtaaaatcg gtgattctta
    32761 tttaccgtca gagatttatt ttgactcagg aacgctagtg atgaatggag tcaatgcaat
    32821 tgaacaagca caatttcatg atcgcgcgca taaagaaatc attgatttgg cggtaacagc
    32881 tgcagaaaat ccaatggcag atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa
    32941 agatccagct tggggattgt tgtatgaaga gtggatcgag ctagctagaa atgtgaacca
    33001 agtgatttat gatttaagaa aaaatggcgg catcaaattc gatgcagata atgaggaaga
    33061 tttttaatga acgaatttgt acaagtattt cccgaaaatt ggcagaaaaa atatgaggat
    33121 aaagggttta tctcaccttc tcttattcaa cgagcagttt ttcagccact aagtaataaa
    33181 caaagtatag tagcggtttc tcctacaggt tcaggtaaaa cactggctta tctttggcct
    33241 ctactactaa acgtggaacc aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa
    33301 gaactagcta ttcaagtagc agatgttgca cgtgaatggg gaaaagacaa agagttgaaa
    33361 gtccaatctt tggtgggcgg agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa
    33421 ccagaaatct tgattggtac accaggaaga atattggaat tgatgaaagc aaaaaaaata
    33481 aaagcacatc aagtgaaaac aatggtattt gatgaagcag accagctatt tgacgctggg
    33541 aacagccaaa tcattgatca aattcttcat caagctccaa cagagtatca gctagctttt
    33601 ttttcagcga cagcagatcg ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt
    33661 gagacaattg acgtgacagc agaggacgat tctcgaaaag gactgagaca ttacttttta
    33721 cgtgtaccga tacgaaagaa agatgactac ttgagacgtc tcacgcatat agaagatttt
    33781 catggtctga ttttctttaa tcaactgact gaattaggcg taatggaaga aaaattgctg
    33841 tatagaggtg taccggtagg aagtctggcc tctgatcaga acaagctact ccgaaaagca
    33901 gcgctagaac aattcaaaaa gaaaatggtc agagcacttt tgaccacaga tgtggcggct
    33961 agaggtttag atattacagg tcttccttat gtagtgaatg cagaagttcc gttaagtgaa
    34021 gaagcttatt tgcatcgttc gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt
    34081 acactcgtac aagataataa tctaagagat ttaaaacggc ttttacgtcc atcaaatata
    34141 acacttgaag agattttttt atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa
    34201 gatgcagctc ctgcagtaaa taaaaagtat gcgaaaactt tttctggaaa ggagcagaac
    34261 ccagaatata acaaagaaaa aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga
    34321 aataaaggag caaggcgcaa ataagcgcct ttttatgcta tgattatgaa aacgggaaca
    34381 aaattggttg ccagaggagg ggttatgtga aagaacgtca agaagagatc ttgcagctat
    34441 tgacagaaaa ccaacaagga ttgacagcaa gtgatgtagc tgagcgctta actatcgatc
    34501 gaagcaatgc aagccgatat ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg
    34561 ctggccgacc agtcgtttac tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg
    34621 tcgactcttc tacgcaagtg acttttgaaa cactcgttgg agaaaatgat tctttaaaag
    34681 tcagcataca gcaggcaaaa gctgcgattc tttatccacc gcgggggttg catacgatta
    34741 tttttggaga aactggtaca gggaagtcga tgtttgctga atgtatgtat cattttgcaa
    34801 tcgattcaga gatgctttct gcagatgcgc catttgtttc tttcaattgt gcggattatg
    34861 ctcaaaatcc gcaactgctc tttggacaca tctttgggat caaaaaaggg gcttacacag
    34921 gagcagctca agatagtccc ggtctaatag ccaaagcaga cggcggtata ttgtttttag
    34981 atgagatcca ccgtttaccg ccggaagggc aggaaatgct gtttactttc attgataaag
    35041 gtatttaccg tccgcttgga gaaagtgcac aagtacatga agcatctgtt cagatcattg
    35101 gggcgacgac tgaatcttca gaaagtttct tatctacgtt caatcgacgg attcctatgg
    35161 caatcacctt gccgaatctt gcttcacgtt cactggatga gcgttatgaa atcatctctc
    35221 tttttatcaa acaagaagcg aatcgtttga atcaacgtat cgatatcgaa aaagaagcga
    35281 ttcttgcctt catgctttat gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga
    35341 aactagtctg tgcaaaatct tttttacatt accgtactca tcatgagaaa aagctaatca
    35401 ttcgaaaaga agaactttcc cttcaagtac aaaaaggatt actgaaaatc aaggaaatac
    35461 cggaacgtct cgaccgattc atggacagca aaagtcagta tttgacattt gaaccaggat
    35521 ttgctgatgt tgtctggtca caagatccag aacgaaacat gcaggtctat aacgatattg
    35581 aggaaaaagt cacttctctt tccgaaaccg gtgtggagaa cattgatcta gagaccctca
    35641 tttcaaaaga tgtggatgct tatttccaga catatgtcaa agaattgact aaaagttcga
    35701 ttcccaaaga tcttttgcca gatgaaatct ggcggttaac caatcaattg tatgatgtgg
    35761 ctgaaaaacg gttagaccgt atttataatg aaaaagctcg ttttgctttt gctttgcatc
    35821 tgcaaagtac attggatcgt gtgagagaag gtcatatgat cgtccaccca gatttgaata
    35881 acgtacggaa aaattttaag agggaattcc aggtagcaat cgatctttca acgatcattg
    35941 aagaagaaga acaagttgag attccttttg atgaaatcgg ctttattagt atgtttcttt
    36001 ctatcaatct aggagaaagt gagcctgtta aagaaaacaa agtagatgtt ctcatattga
    36061 tgcacggcag acagactgct tcaagcatgt tggagatggc acaagaacta ttaggaacaa
    36121 aagtagggaa agcttttaat atgccactgg ttatggaagt tcaaacaatg tatgaagaag
    36181 tgttacaata tgtcaaaaac tatcaagagc atttgacgaa tggcctactc ttgttaacag
    36241 atatgggatc attaaacacc ttcagtgatt tgatttatga agagactggc attcggacga
    36301 aagctatcac gatgacaagt accatgattg tgatagaagc gatacggatg gcaagtgttg
    36361 gaagaagctt agaagatatt tatcaaaata tccagctttc ctttgaatcg atcgtacgtg
    36421 atcagttcaa agcggaaaag tctgaaactt tacagcgtaa aaaagccgta atcgtgacat
    36481 gctttactgg tgaaggagta gctgccaaat tatatcagcg tattatgcca gtgattaatc
    36541 aaaataaggt agaactgatc cagatgcagt ttttagaacg tgaatcattc aaaaaacaca
    36601 tcgatgcctt attggaagaa tatgagatca aagcaattgc gggaacagtt gatgttgatt
    36661 atcaaaatat cccatttttt tcagcctatg acgtttttga tgatgaaaaa ctaaatattt
    36721 taaagcgtat agctgatgat gagatcccga cagctaaaat tgtcgattca cttgctggat
    36781 cattaagcga tgtcttttct attgatgaac tgatccgttc attgcaaaag acagttcagc
    36841 agatccagac cgatttgtcg attatcgtgg aacctggagt agatactgga atcgttatcc
    36901 atttagcctt catgttagac ggattgatca aacaagaaag ccatcggcaa tttgagaaac
    36961 tgtctatttt ctcaaaaatt tatcgtttgg aaatggatat cgttcgggca aacttgatgg
    37021 cactagaacg aaaatatcag gtaaaaattc cagaagatga tatcgcattt cttacacaaa
    37081 tgtttcttga aaataaaata aaaaaatgaa cacactaatc atactgttta cacactaaac
    37141 atctgtgtgc gcactataaa aaagagctaa aaagcatgaa aacgctattt cagctctttt
    37201 ttttattggc atgcttcttg cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa
    37261 atggcaaaaa aaacaatcat gttagtttgt tcagcaggga tgagtacgag tttattggtc
    37321 acaaaaatgc aaaaagcagc agaagaaaaa gggatggaag ccgatatttt tgcggtctct
    37381 gcctcagatg cagataataa tttagaatct aagaatgtcg atgtattatt attaggtcct
    37441 caagtacgtt tcatgaaagc tcaatttgaa caaaaacttg cgcctaaagg tattccatta
    37501 gatgtcatca atatgcaaga ctatggcatg atgaacggag aaaaagttct tgcacaagca
    37561 gaaaatctaa tgaaataaat caagtatgca gttgggggta taacaacgtg gaagatcaac
    37621 aaaatctaga agccatcatg gggttaatca tgtttggcgg taatgcaaag agcgatgcaa
    37681 tggaagcgat tgcggctgct aaaatcggtg attttgagtt agcggatagg aaaatcgctg
    37741 atgcagaaga atcacttata caagcgcatc atgcacaaac agacatgttg acacaagaag
    37801 cacaaggaaa tcatatgcaa gtgacattat tgactgttca cagtcaagat cacttaatga
    37861 catctattgc gtttactgat ttagcaaaag aaatcatcga actttatcgt cgcgtataca
    37921 gcgaataaat acaacttctt ttttgaacgg aagaagaaaa atttttgaac ggatttttta
    37981 ttttgttata aggagaactc tctttatata aaaaacttat aggaggaagt aaaaaatgaa
    38041 cggactatca agttggatgg agaaatacat cctgccggtt gccgcaaaaa tcggagcgca
    38101 aaaacattta gttgctttgc gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg
    38161 atcagtcgct gtaatgatca atgcaattat tcgtgattta ccgcaacagt ttaacagtac
    38221 ctatgctgac agtttagcag cagacaaagg aatctttgcc gtattgaatg tcatcatcgg
    38281 aatcaatggt tttgtctgga acggtacact agcaatcgcc ggattgatct ttgccttttc
    38341 ttgggggtat aatcttgccc gagcttatgg agtaaatgat ctagctgggg ggatcgttgg
    38401 tttagctacc ttgattcaag gtatttcctt tggtccagca ttatcagcag cattagatgt
    38461 gaatgttcct tcaaatgtcg cagatgcaat caatagttct gagattggtg caacgattgc
    38521 agatggcaca ctgtcagttg gattatgggg ttggctaaaa ctagatcatt tgaatggaaa
    38581 cgcttatttt acagttatga tcatgggagc tatttcagtc attatttttt gtaaattgat
    38641 gcttgccaac attacgatca aaatgcctga ttcagtacca ccagcagtat ctaaagcatt
    38701 tgcagccatt ttaccagcga cgatcgcttt atacgtcatt gctattatca attatatcgt
    38761 aggtcgtgta gctgatgggc aactgatcat tgatttggtg caaaaatata ttgcagaacc
    38821 attcctagga ttatctcaag ggttgggagc agtattgatc gtcacagtat ttgttcaagt
    38881 attctggttc tttggtattc atggatcaaa tgtattggca ccagtgctag agggaatctg
    38941 gggacaagca caactaatca atattgatat tttccaaaaa ggttataacg gaaaaactgg
    39001 tacaccagcc gtgttagatg caatcgaaga tggaaaagct tatatgtggg tccgcggatc
    39061 gttcgatgct tttgcatggt ttggcggatc aggcggaacg atcgtcttac tgatagctat
    39121 cttgattttc tctaaacgtg cagattatct aacagtagct aaattatcca ttgggcctgg
    39181 tatcttcaat atcaacgaac cagtgatgtt tggtctgcca atcgtattga atgcaatcat
    39241 gtttatccca ttcttgattg ctccagtagt agcaacagca attggatgga cagctactta
    39301 tcttggccta gtcgctccgg tatcgcagca agtcacatgg gtagtaccgc cgttcttatt
    39361 gtcattctta gcaacaggag cggattggcg ggcaccgatc gtaacattag tatgtatggt
    39421 cgtgactttc ttgatttggg caccatttgt catctctgca aacaaaatgg accctgccgc
    39481 aggcgaacaa caataagatg aacgtgagga gcgggacgct ttaagtttcg ttcctcataa
    39541 tgaattttcg aggaggaact gaaatgatta caattgccag tgcaatgcaa caagatgaaa
    39601 tcaaagaatt gttagaagga tacaatgaag aaggaatgac ttttacattt aaagaaaaac
    39661 aaggaatcaa gctgttcttt gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag
    39721 caaaggaact aatcaaagca cagccttggg gaacagtcct ttattttcaa tcaacagctg
    39781 tttagaaaat ggtttaaaaa gataggatgg ttgagatgat aaaaaacggt ttatttttat
    39841 gtagtattgg ttctttgatc gtgttgtata gtttgaatcc acattcgcaa aggtatgatt
    39901 tgtatagtat ggtcacaggt ctttttctaa caggattagg aatctatttc ttcatgaagg
    39961 ggaaaaaaag agaagaagca aaggagaaaa ataactaatg gaaatcacga aaaaattaaa
    40021 tattccagca tcgttttttt acgataaagt tatggactct gtccttttcg atgtacgtaa
    40081 agcgaccggc aaatcagtaa ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt
    40141 ctcgcaaaat aaccgggctc gcataaaaat cgaagaagtc attgaaaacc agtcttataa
    40201 gtttcgtaca tcgacaacga agaatgaatt cgtcgttcat tatcaaatca aaccattgga
    40261 cgattcgacc tgtgaggttc attacacaga aaaaatggag tcttatggca tgcttcaaaa
    40321 gttgaatgat atgcttttag gaacaatctt gatgtatttt aaacggagac gtttcaaaaa
    40381 aatgctagat atgatcgaag caacgtatta aatcaatgaa acatggtact atttagttat
    40441 tgaaaaatga agtaagctag aaggaatttt tcataggaag atagatcttt cctgcgaatc
    40501 ctcactagct tttttgtagg aaggggcagc aaaatgacaa acaaagtaag agagctaggt
    40561 gtatcttcga ttgaggaaat gtttgattta gccacttatg catttaatgg ggcagataca
    40621 cctgaaaggc gagaacgttt tcgtacactt gcggagaatt cttggaatta cggatttttt
    40681 gatgaggagg atcggctaac aagtcaagtc atggcgaccc cttttccagt gaatttttac
    40741 ggacaagagt acctaatggc cggcattggc tatgttgctt cttatccaga agcaagggga
    40801 caaggcggga tcaatcagat catggaaaag atccttgaag attgccgtga tcgtaaagta
    40861 agcttatctt atctggcgcc attctcctat cccttctatc ggcgctatgg ctatgaacaa
    40921 tcatttgata aaatcagtta tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa
    40981 tctggaaaaa taaagcgtat ggattttgaa gatgcaaaag ctgcaatcag aagaatttat
    41041 gaacagatgc cggaaaacca acgagctggt ctgaagcgcg aagactggtg gtatacgtat
    41101 aaattcaagc aaaaaggcaa ttaccagttt gctttgcatt ttgatgataa tgaggaagca
    41161 acgggatata tcgtttatca gcttgcaacg tcttccttta ttattgcgga gtggggattt
    41221 ttggatcatg atgctttttg cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt
    41281 gaaacatttg aatattcatg cggctacggc ggtaatgatc aaaattatct attagaaaat
    41341 ccatttgctt ctgtaatgat cacaccttat atgatggcga ggatcgtaga tatttctttg
    41401 tttttggaaa attatccatt taaaaagaga caagctgcat ttgccctaca aatagaagaa
    41461 gatcattatg cgaaatggaa tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat
    41521 catctctcaa aagtagaaca aacaaaactc ccaatcgttc gaggaagtat ccaacatatc
    41581 acgcagctat tattagggta tcgtaaagtg gaagagttgg tattccaaga aagaatacag
    41641 gtgacaaaag aatatgaaga aacgttaagc tcaatcttgc cacagcaaca gccaattctt
    41701 aatgactatt tttgataaaa aacgggtgat gacaatattt ttgtcaccac ccttttgttt
    41761 ttcatttggc atttgaagga aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt
    41821 ttttgttttc ttttgtttta gggttttctt ttttgtggtc ttggattgct tctttcgtaa
    41881 tcttttcccg atttttttta gatgaatcta agcgttctgc gtatactttt tcttttggct
    41941 tatcattcat tggaaaggac ctccctacat tatttcattc tacctctagt ttatcatctg
    42001 ttaaggacta gtggaaacgt tttgcctaac ttataagctg atttttttag cagataagct
    42061 tgcaagtaaa aaacaaacaa gctacaattg atttattgaa attgttcctg tagctcagta
    42121 ggatagagca atcgcctcct aagcgatagg tcgctggttc aattc
//
LOCUS       DACCQI010000006        38378 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCQI010000006 DACCQI010000000
VERSION     DACCQI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.006"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..196)
                     /locus_tag="IVO76_000264"
     rRNA            complement(<1..196)
                     /locus_tag="IVO76_000264"
                     /product="16S ribosomal RNA"
     gene            116..259
                     /locus_tag="IVO76_000265"
     CDS             116..259
                     /locus_tag="IVO76_000265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428260.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(719..2029)
                     /locus_tag="IVO76_000266"
     CDS             complement(719..2029)
                     /locus_tag="IVO76_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7428261.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(2041..3459)
                     /locus_tag="IVO76_000267"
     CDS             complement(2041..3459)
                     /locus_tag="IVO76_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7428262.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3699..5267)
                     /locus_tag="IVO76_000268"
     CDS             complement(3699..5267)
                     /locus_tag="IVO76_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7428263.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5271..5681)
                     /locus_tag="IVO76_000269"
     CDS             complement(5271..5681)
                     /locus_tag="IVO76_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7428264.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5678..6601)
                     /locus_tag="IVO76_000270"
     CDS             complement(5678..6601)
                     /locus_tag="IVO76_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP7428265.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6598..7476)
                     /locus_tag="IVO76_000271"
     CDS             complement(6598..7476)
                     /locus_tag="IVO76_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7428266.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7463..8800)
                     /locus_tag="IVO76_000272"
     CDS             complement(7463..8800)
                     /locus_tag="IVO76_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7428267.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8803..9906)
                     /locus_tag="IVO76_000273"
     CDS             complement(8803..9906)
                     /locus_tag="IVO76_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7428268.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9980..11488)
                     /locus_tag="IVO76_000274"
     CDS             complement(9980..11488)
                     /locus_tag="IVO76_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7428269.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11454..12146)
                     /locus_tag="IVO76_000275"
     CDS             complement(11454..12146)
                     /locus_tag="IVO76_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7428270.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(12168..13142)
                     /locus_tag="IVO76_000276"
     CDS             complement(12168..13142)
                     /locus_tag="IVO76_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7428271.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13442..14314
                     /locus_tag="IVO76_000277"
     CDS             13442..14314
                     /locus_tag="IVO76_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7428272.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14772..16970)
                     /locus_tag="IVO76_000278"
     CDS             complement(14772..16970)
                     /locus_tag="IVO76_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7428273.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            17202..18194
                     /locus_tag="IVO76_000279"
     CDS             17202..18194
                     /locus_tag="IVO76_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7428274.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            18254..19675
                     /locus_tag="IVO76_000280"
     CDS             18254..19675
                     /locus_tag="IVO76_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7428275.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19668..21167
                     /locus_tag="IVO76_000281"
     CDS             19668..21167
                     /locus_tag="IVO76_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7428276.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            21206..22600
                     /gene="uxaC"
                     /locus_tag="IVO76_000282"
     CDS             21206..22600
                     /gene="uxaC"
                     /locus_tag="IVO76_000282"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7428277.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22759..23442
                     /locus_tag="IVO76_000283"
     CDS             22759..23442
                     /locus_tag="IVO76_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7428278.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23444..24280
                     /locus_tag="IVO76_000284"
     CDS             23444..24280
                     /locus_tag="IVO76_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7428279.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24291..26045
                     /locus_tag="IVO76_000285"
     CDS             24291..26045
                     /locus_tag="IVO76_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428280.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26321..26662)
                     /locus_tag="IVO76_000286"
     CDS             complement(26321..26662)
                     /locus_tag="IVO76_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7428281.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26730..28901)
                     /locus_tag="IVO76_000287"
     CDS             complement(26730..28901)
                     /locus_tag="IVO76_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7428282.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            29112..29969
                     /locus_tag="IVO76_000288"
     CDS             29112..29969
                     /locus_tag="IVO76_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7428283.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(30040..30897)
                     /locus_tag="IVO76_000289"
     CDS             complement(30040..30897)
                     /locus_tag="IVO76_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7428284.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30882..33584)
                     /locus_tag="IVO76_000290"
     CDS             complement(30882..33584)
                     /locus_tag="IVO76_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7428285.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33597..34886)
                     /locus_tag="IVO76_000291"
     CDS             complement(33597..34886)
                     /locus_tag="IVO76_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7428286.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            35036..36082
                     /locus_tag="IVO76_000292"
     CDS             35036..36082
                     /locus_tag="IVO76_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7428287.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            36084..38237
                     /locus_tag="IVO76_000293"
     CDS             36084..38237
                     /locus_tag="IVO76_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7428288.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11307 a         7904 c         6828 g        12339 t
ORIGIN      
        1 gattgttata cggtattagc acctgtttcc aagtgttatc cccttctgat gggcaggtta
       61 cccacgtgtt actcacccgt tcgccactcc tctttttccg gtggagcaag ctccggtgga
      121 aaaagaagcg ttcgacttgc atgtattagg cacgccgcca gcgttcgtcc tgagccagga
      181 tcaaactctc ataaaaagtt cgaacaatct tgcgattgtt aagctcaatt tgtttgctag
      241 catattgctt gcttgttaaa aatgtttatt gtcttgaatt gaatcaagac gccctacaca
      301 tttggtttgt cttactttgt tcagttttca aaggtctact tcatcgagag tgtttcccga
      361 ttcatttttg gctgccgtag caacttttat atcatatcag agagacaaac gattgtcaac
      421 tcttttttaa aagttttttt cgatttcgtt tttcgaaatc tttcagcaac tcatttatca
      481 taacttatcg tttgttattt gtcaagaact tttttgaagt ttttttcgat agaatcaagc
      541 ttcgcaagtg gttcttttta acgacttagt tatcttatca cgtctaaaaa catgtgtcaa
      601 ctcttttttg gaattttcat aaaatgtttc aatgaagcgc gacaacgttg tttaatatat
      661 cattaaaaaa aataaaggtc aagctaaaat attatttaag cttgacctta ttataatttc
      721 aacgattgtc aaaagtattg tttgcagctg tgttgccagt ttctatagat aaatcacagc
      781 ttttgaaaca taagttttgt actgcttcga ttcgcccgaa ttcttttgca gttatttggc
      841 aatgagaaaa gcagacatct tctataggct tttcaggaaa tgctactaaa tcaaaagctc
      901 gggaaggaag tgagtaacct ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt
      961 tgactttcgt catttgcttt gattcaggta tttcttcagc cactgcttgc caactttctg
     1021 gcatttgagc aaggtcattg ctattttttc tattgtattc attatgccag tccattatcc
     1081 aagaaaaagg aaattgtaca tcaatcatat tcagattttc taccctgata ttttcgatca
     1141 ctccccctct ttcttttgaa gatttcattc gaataccaca gtctgactga aaaaaatcga
     1201 tatcgtgaat atatacgtca ctaactccag cactaacttc gctaccaata gttacaccgt
     1261 atcctgaacg gattttacat ctagcaacct cgattcttga tgattttcga ttgactcttg
     1321 caccattgcc atctctgcca gacttaatag caatacaatc atcaccacag gccaaatcac
     1381 attcatagac acgcacattt gttgaagaat caatatcaat cccatctgta cttggtccat
     1441 cattgttgcg gatgattact ttttctacta atacatcgtt agaataagtg atttgcaagt
     1501 tccaaaaacc tgatttctga aaaatcaaat cacgaacaac aacatgttct gcatgataca
     1561 gtaaacaagc gcgtggacgt ttaatagcat aatccgcaat ccaacgaagt cccttttgat
     1621 cgtattcagc tcttgtcccg cttttttgat cttttcccca atacagctcc caccaatggt
     1681 ctccttgacc gtcgattatt ccttttcctt ctataagaat atctttcgcc gagagaacat
     1741 tcaaaatcgc tgctggccat ttcatttcta cgccggctac tcgtgtatcg atttctggaa
     1801 atgcttcgat atctgttctc cccttgagga ctgcaccttc ttcaaagata agatgactct
     1861 tatcttttaa aaaaagggct ccgactaaat aaatgccttt tggaataaat acaggtaagt
     1921 gttctttcgc accttgatcg attgcttttt gcagtattat tgtatcatct tgttgagtgt
     1981 cgtttgtttt aaagttcaaa gcattgataa tccctttttt tgtccgcaat acgtttctcc
     2041 ctatctttca ttgatttttt tcaattcttc accaataaat acagtttttg aacgattcag
     2101 tgggtaacct actagcaaaa tgattgcgga tacaaataag cagaatgctg gtaacaagtt
     2161 tgccatcgta taaatccttt cttgtactaa aactgattgg atggctccac ctgttgtaga
     2221 cgattggtaa ccaatgagtg ccagcataaa tccgcctacc gctcctgcac atgcttgtcc
     2281 tacttttctc gcaaaagaat ttactccata tactgttcca tcttctctta agcctgtctt
     2341 atattgatga taatcaatca cgtcagtaat gaatgcccag accatcaaat tgaataaacc
     2401 agatcctaaa gtagcgataa ataaaagaat cagatatacc catgcatttg ttatatgaag
     2461 gaaatacatc agcaagtaca tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt
     2521 tttaccaaat aattttgtca gctttgtagc aaatggtgct aaaactacta ctgttccgta
     2581 agtaaataaa agagcaacag acattgcttg tttattatgg aagtaatcat tgaataaata
     2641 agtcatattc gtcccagcta aaatctgatt gatcacaatg aaaatgtcaa caatgactaa
     2701 aataatcagt gctcgattag ctaacaaacc ttttaccaac tgagaagcag atactttttc
     2761 gtttttgaca actcttactc tttcagtagt caatttacaa gttaacatat atgcgacaaa
     2821 tgctattgca gcacatccga ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg
     2881 tccactcttg ttatgggtat acattaaaat cggaatgaag aatcctgtag ttgatccgcc
     2941 aatcgcagaa ccaatgcttc ggaacgtgga caacgaggtg cggtcattag gatttgagct
     3001 aattgctgct gccattgctc catacgggat attgattgtt gagagaaaaa tcccataaac
     3061 aatgtatgaa acaaaaatat aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg
     3121 caaaaacaaa atgacagtca ctaagcaaaa tggatacttc atttttctga tccaaggaat
     3181 aaatcggcca gtttctgtta actctgagac atcacataat cttccaacca tcacatcagc
     3241 aaacgcatcc aaaattctcg aaatcaagaa taacgtacct acgattgccc ctgaaacgcc
     3301 aagaacattc gtataataaa tcatcaagaa actattgact aaacctaaaa taaaacagtt
     3361 acctagatca ccgaacatgt agcctatctt atcggaaatc ccaaattcct ttttttcttt
     3421 ttttattgta tttgctgtaa cagcattttg tgaaagcatt tgaattcctc ctacctcttc
     3481 tagtaaacgt tttcaaaatt ataactcttt ttttcttgtt tttatattca caatgttggc
     3541 tttttattgc attatcttga cagataaaaa acaaaaaata atctaaaaac attgatgcat
     3601 aagattgata acttttttta cataacaata tatacaaaca aaaaaattca ctaactagtg
     3661 aaaataagtt agtgaacctc atttattaaa gaattaaatt acagatgatc ttttccatca
     3721 tacttgaaaa actcaaaatc agcataactt cctctttttt tatttagatc atgcactgct
     3781 atcccgataa aatttccagt aaaccctcct gagagtatac acatgttttt ttgcggacct
     3841 actttcttcc attcttcagt tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt
     3901 acagtgactt tcaaaaaagt cttcttttct gtgagtggaa tcgtccaatc ttctatttgt
     3961 gctactccgt tttctgattc tatcatacgt aggcattttc ccttgtcctc attccaagta
     4021 acataacaat aaatataatt ggattcattt aagtacagta ccagtccagc catctgatta
     4081 aaattattag gcatgtattc aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga
     4141 atagctaaca aatgatgttc gaaggtagat tgaagagatt caccggaaat cagacgtaaa
     4201 aaacctggtc tacattttgt atcacaccaa tcagcatttg gcaaaatgcg tctggcattc
     4261 cagttcttct ttagctctcc ttcaaatgta tccataaact gatgatcttt ttcttgttgt
     4321 acctcttcat ttgtctcaat cattatttct tccatcggag ttgtctggct atctttaagt
     4381 cttaaccaat cgtcggacgt ccaatacact tcttgaatag cggtttctct tcccaaaata
     4441 gccgcaccgt ttaatggtct ggtacaaaga tatgtcatgt accatcttcc tgacggggtt
     4501 tgtactaagc tcccatgtcc tgtacattgc aatgtagaag caggtttgtc actagctgtc
     4561 agcataggaa aaccaggatc aagctcgtaa ggaccagtaa tattctttgc tcggcaaact
     4621 gtaactgaat gtcctcttcc tgtaccgcct tctgccgtca tcaaataata atacccgtta
     4681 cgaaaataca aatgtggtgc ctccgttttc gctaattctg tacctgcaaa tattttatag
     4741 atgggaccat caagcttctt cttctctgca tcatattctt gtaatacgat accgctggat
     4801 ttattcggtg tgtccagacg ataatcccat agttcattga caagccattt ttttcccgtt
     4861 tcaagatcat ggaacaaaga aggatcaaag ccgttactat ttaaataaat tggttcggac
     4921 caaggacctg tgatctcaga tgcagtgatc aaataattgt ggacatcttt aaacggacgg
     4981 cctgcacttt tgacatctgt gtaaacaaga taaaacaatc catcagcata actgatctgt
     5041 ggcgcccata tacttccatt tgtaggattt ccttgcaaag atacttgagt ggtcaaaata
     5101 tccgttgtat gtgaccagtt gactaaattt ttcgactcat atactcggat tcctggaagc
     5161 cattcaaaag aggagacaat aatgtaatac cgctctttca ctcttaatat attaggatct
     5221 ggattgaatc ctcttaaaat aggatttttc actttgatag tctgcatgat tcaggctcct
     5281 tttctcattc ttactcgata taaactgaat gccccagctg cgaaataaaa aaatgcaaaa
     5341 ggaaacgcaa taaactgagt gccaatcaac aatatgaatc ctgttataaa atacaaacca
     5401 gtggcagttg ttaagtattt tcctttagca gtcaaaaaca atgctactgc taaaaaagca
     5461 agtgaaataa acaacacaac ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta
     5521 ttttctaaag acaaagttga atgatctaca gcaagcgact gataactttt tttaaactct
     5581 gacgcagaca tattacttaa agtaactgct aacccgccaa agaaaatcaa agatagactg
     5641 ccaccgacca ttcctagcaa tagttcccat tttcttttca tttctgaacc ccctgctttt
     5701 ctaaatgtat ccattcaggt cttttgatcg atgtttcata attgttatta cgttcagaga
     5761 atttgacagt ctgttcattt ttcttgtcat cccaattatc ccaaccagtt gggtgaacat
     5821 gcccacctat cttacacgct tcaaataaga catttgcata cggacgccac ggtcttccta
     5881 aatagacgtt tctagtatca ggttctgcag tcaaggtaca gctttcaaaa cgataccctg
     5941 ttttttgatt tttcggcgta gatgctgctg tgatgtatcc aggattttta ctggctaagc
     6001 tatggatatg acactgatga aaaacggcat ctgccccacc aaatatgaaa tccaccgttc
     6061 cttcaatcca gcaatattca taggtctgac gacaatactc aggattggca gaagccggtg
     6121 tacaaaaagg agtaccatct ttttgcaaat caggcaacgg acccgtgcat aaggtatcct
     6181 gataaccaga tagtcgacag ttttttaaag atacgcgatc tcctgaattg aataaagcaa
     6241 cagcctgtcc taccttttct cctggacctg ctgtattttc gatgtgaaga ttttctaata
     6301 cgacattcga tccttctaag aaaaaagtag cacttcggaa agtccctaac tggctaccgt
     6361 caagatactc ctgttttgca aaacgattac cggagataac tactgttcct gctcccacaa
     6421 gtttaaaatt ggataatctg ctttctatat attcatcata tcttccttct tttactatca
     6481 agacctttgg atggctatcc ggcaggcctg ccaaataatc gattccttct tggatagttt
     6541 caaaatccgt cggggtatct atagcattct ttccaattat cagtttgtct ttcatcctca
     6601 taaatgctcc cttatgaatt gtttccaaat atttctcatt tcttgtgttt ccatatgacc
     6661 gcctgttaaa gaaaaagaag aaaagttttc tggactaccc attgattgat actttttgtc
     6721 cagccctttt ctcagcttca gtacgccttc gtccaagcac atcgtatcgt tttttccaac
     6781 taagctaaga cgagggcgcg gagcgatttt ttcttggatt gctaacgttg tataagctgt
     6841 caatagattt ggcacatagt aataaaaccc atgatgatct aggcggcgat gtagcaaaag
     6901 tatttcgatg tccacctgtg ccgctagatc aatacaaact ttgacacgag aatccaatgc
     6961 tgtcacccac cagcttaata agcctcccat agaaagacca atcgttgcga gccgttgagt
     7021 atctgtgtct tcccttgtct ccaaataatc gatcaagctg atcacatcat aaatacgcat
     7081 tccccataac gtttttcctg ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc
     7141 tccgcgttct tcaaatcccc aagcatcaat cgaccaaaca gcatatccca ttcctgtcag
     7201 tacttctgca aaagatggag aagctaagta aggagcacca tggatcaatt cacttttccc
     7261 gactgaaaaa tctccgccgt gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt
     7321 ttctggataa gcatagaggg ctggtactgt ttccaattca tttaagtaca attgatagta
     7381 ttcaagcata aaaccgtttt gtttttctcg gtataatcgt tttccactag gggtatgtct
     7441 gctagatgga gcacctaaaa aatcattcaa ctgcataagc aaagttttta cgaacaacca
     7501 gttcatccat attttctgat tgatttgatt ggaatttctc aatactgatt tctttagtat
     7561 tttctaatag aaatgctggt ccctcgtgat tttcaatcga catctggtta aaggaaacct
     7621 tacccgcaaa gccaatataa aatccttgtc tgctcatttc ctctattcct gtcatcatag
     7681 caggaacacc gggaactgca tcttgggcca tcgagacatc gatattgtca aatgtgattt
     7741 cttgtatgaa ttgttccgct aatccataaa taaaacctgc agaagcatgg acattccttg
     7801 ccgtgatatt cgaaaaatgg atccttctga atgctggtgt ccgttcatct atcggataag
     7861 cctttttttc ccatacatac ggttctttcc ctcgcggacc acagaaataa taaagattca
     7921 gtataaatgg acacatcaca ttatccatga ctatattgga tactcgaata tcttcaacga
     7981 tcccgcctcg acctcgtcta gatttcaatc gtattcctcg atcagtttct tggaagatac
     8041 aatttgaaat ggtaatatta cgaatactac cactcatttc acttcctaat acgacaccgc
     8101 catgtccatg gaccatcgtg caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt
     8161 acgtatcttc agtccctgcc ttgattgcaa tacagtcatc tcccacgtca atatggcaat
     8221 tactaatacg cacatttttg catgattctg gatcaattcc gtctgtattt ggtgaatccg
     8281 ctgggttcaa gatcgtcaag ttatcaaacg ttgcattact gcaaagaatc gggttgatcg
     8341 tccaacttgg tgactggatc aatttaatat ctttcacagt aattcgatga cagttatgga
     8401 aactcatcaa tttaggacga ggataagcga gattatctgg ttcatttcta aaagtatgcc
     8461 accatttttt tccatttcca tctaatgtac cgaaaccagt gacagagatg ttctctacat
     8521 tttgagcata gatacaggaa gcataaactt ttcgatggac tccttcccaa cgtgaatgaa
     8581 caacaggata atcttttgga tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa
     8641 gttcgacatt gctttttaaa aataaagctc ctgttagaaa ttcacctgct ggaacaacta
     8701 cggtcccgcc tccgtcactt gctgcagcat caatggcttg ttgtattgct tcagtattca
     8761 attcatcaat tgatgcgcca aatgtcaaaa tatcatacat atttataatg cctccacttc
     8821 aatcaatgct tgcaagaatg caccaacgcc tttttgatca ttacaaataa ttggttcact
     8881 gatgtagtaa gcatatgaac catctcgctg atctgctccg cctaaccctg ctacttgaca
     8941 gtttttattc aagttcagcc agccttcttt tgtttccaga acaaattcat caagtaatcc
     9001 ttgatgtgat ttcttcactt gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat
     9061 tcctttagcc attgcgtaaa caaacatact actgccggaa gcttccagat aatttccttt
     9121 ccgttttgtt tctgttgtta cttgatacca tgtcccgctg ttttcttctt gatatggaag
     9181 taatgcagcc agtgtctttt ctagaatttc tttcagcaaa gaacaatctg tttcttcatg
     9241 aagtaattca taggtatctg ctgcagccat caaataccag ccaatcgatc ggctccaaaa
     9301 atgatgggat agacctgttt ttggatcagc ccatggttgg accttctttt catcccatgc
     9361 atgaatcagc aaatttgttt ttggatcgaa tagatgttta taactgattt caaactgtct
     9421 caaaacatct tggtaatctt tttcttcact aaaattctgg ataaattcag cataaaaagg
     9481 agaacccata tataagccat ccaaccaaat ttgataagga taaatttctt tatgccaaaa
     9541 agctccttcg gatgtgcgtg gatggtttgc caactgctgg gaaaggagtt ctgctgcttt
     9601 tttatatttt tcttcgcctg tttctttgta caataaaaag aaaagctttc ctgtatttac
     9661 atggtcgata ttgtattctt caacgctgta gccacggatc gttccatctt cttgcacgaa
     9721 atagtcgata ttgtctttta tgaaatcaaa ataaattttt tcttgggttc gttcccaaag
     9781 taattggaac cctttaagta cgacgccata gtcataagac cattttcctt tataccactt
     9841 ttcttcatat aagcgaggtg ttctttccat gatacttttt gctaatttct ttgaccagtt
     9901 ttccattatt tgctctcctt taaaaattaa aataggattt gggacaaata ccaagaagta
     9961 tttgtcccaa tcgtctatat tactgtgccg ctgctttgta tgcttcctcg tactcttgtg
    10021 tgattcgatc gcctccttgt gctttccagt cttctacagc ttttttgaat ccatcttcgt
    10081 caagctgtcc catgatgtat tgcaccgtag catcaatgac gattttttca atttctgatc
    10141 ctacttcatt gttcgtcggt gaatccaatg gcacagaagg atccattaca gcaaattctt
    10201 cattttcttt gatcaattga ttagagaatt ctttttcagg gtctgtatct ttgaaagaga
    10261 acgtcacttc actaggacga gagcttgata acggttgcac ctcttgttgc caaagatcca
    10321 tatcgataaa ttcgatagaa ccatcatcat tcaattcata atgcgttcct tcgataccgc
    10381 cagtcatcaa tttatacaca tcttcttcta aaagatcatc aacaaattga agtaatttct
    10441 tcaattgtgc ttcgtctttt acttgagatt tgggaaatgc tagcaagccg ccaataccat
    10501 ttccttctga ccaaacatga taaccgccgc cttcaccatc tgagatcttg tttacaggca
    10561 ctaattccat atcatcttga ataccttgac ccatcgtctt cagattcttg atatccacca
    10621 ttccagtgta gattccagtt cttccttgtg caaattgttc ttgctgatca gtcttttggg
    10681 taactgcaaa gtcttgtgcc aaatagccat tctcaaatag atctcttgaa aaattcaaag
    10741 ctttcatata ctcttctgta tcaaattctg gagtgaattt gccattatcg tctacagccc
    10801 agccatttgg tgctccaaaa taagaagtga aaagtttaaa gctggtataa cgaatatcac
    10861 taggcgcacg atcgccaaaa ccaactgtgt cgtccacacc atttccatca ggatcgtttt
    10921 ctgtaaattg tttagctact tcataaagct catccatggt agttggaaca tctagtccta
    10981 gattatccaa ccagtctttt cgaatgacta agccagcacg agcgtagtct ttttgtatcg
    11041 gtacgccgta caaggcaccg tcgatactcg cagcttcgat tctatcttca gaaatttgac
    11101 ttaaattttt atagtctttt aaatattctg atacatccca gaatacgcct gattttaacg
    11161 cattgcggac agaagagttt ttcaacatcg caatttgaag tgtaacgata tctcctagtt
    11221 catcagaagc caatgcagtt gttaatcttt cttcttttga agcatcagga atccagttga
    11281 atgtcagttc cgtatcggtt ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg
    11341 aagcatgatg caaaatattt aaccaagtga ttttagcttt tccatcttct gtctctgaag
    11401 aactgttact tgaactgctg ccgcatgcgg caagtgctag aattcctgca aaactaagac
    11461 ctaattttac gagcgttgct tttttcataa attttctcct tcactgtttt aataatattt
    11521 ttgttttctt ctgttttttc tgtatatgag ttcttccatt ccaaatggca aaatatagaa
    11581 tttgctgtcc acgtgactgc ggaaaagaag agactgccac cgataaaggt taaaacacct
    11641 ggcattgttt ggatgatcat cagataaatc cctaccatac ttaatatcaa cactaatgag
    11701 taatgaagat gtgagaatcc gaataaaaat gccattttca ttttgaaaaa atatcctttc
    11761 caatcataat gagccatgat tgggaaaata tagagtgctg agagcacata tatgaacgca
    11821 gcaatataca aagcgatcct aagataccaa ttgtgtatga aagaaagatc cacaagtaaa
    11881 acaatcccga tggcgccata tatccagctc atcagcaagg tttcacgata gttttcttta
    11941 aaatatgtga agtacgtttt cgtcaaagga aacgtttctt ttgagcgcag ccattttcta
    12001 atcactgcaa ccatcgcata cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg
    12061 actaaaccgc cccacagaaa attcaaataa accaactgta aaatacgatt tagccttccg
    12121 tttattttat ctaatgactg cagcatatct ttccctctct ttctatctta gtaaacgccg
    12181 tcttctccta gctttttggc aatcttatta gctaaaatta ccataaacaa tcctacgacc
    12241 cctttaaaca atcccattgc tgtactgaaa cttaactgtc cattcttcaa tccagctgta
    12301 taaatatagg tgtcgaaaat ttctgcaacg cttcgattca atgaattcaa taacaagtac
    12361 atatgctcga accctaagtc taatgtctga ccgattttta gaatcagcaa ggtaatgatt
    12421 actggacgaa ttgccggcaa tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc
    12481 atttctgcag cttcatacaa ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc
    12541 gtagaccagc ccaactcttt ccagatcact tgtccgatat atactgtgcg tgtccattca
    12601 ggggatgtta gaaaactgat tttttcaaat cccattgttg caaggaattc attcaatacc
    12661 cctccgtcaa cattcaaaaa tacataagta atcgaaacaa tgattaccca agacatgaag
    12721 tgagggatgt aaatgatcgt ttgaatcccg tttttcaatc gtttgttcgt tacttcattt
    12781 aatagtaaag acaaaatgat tggcatcgga aaaaacacga agatatttaa tccgaacaag
    12841 atcaacgtat ttcttaataa tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt
    12901 ttgaaaccga cccaaggact tcccattatt cctaaaaatg gttgataatc ttgaaaagca
    12961 ataacgagtc cccccatagg aagataccta aaaatcaaga aataaaggac tcccggcaga
    13021 atcatcacat ataaaaattt attcgttttg attcctgcaa ttattttttt tcgtcttctg
    13081 agcttttctt cttttttcga tagcgtcagt tgaaatggtt cttgtttctt tacactttcc
    13141 ataagcccct ccttagtttt gtgaatgttt gctgcttatg acttcatgat agataactat
    13201 ctgaattacg tatataatca tttttaccgt aagtaaaaac gcttacatag caatgatttt
    13261 tgtgctatac aaaacgataa agtgtatttt ttcacaaata aactacgcat agcaaacctt
    13321 ctattttttt ctatctgaca cttagcatag gaaatgatta tatacgtaat ctttcaccga
    13381 tgataaattg aacatataga aacagaaaca tttaatttat tttgaggagg agcctatcta
    13441 tatgaaaaag aaagtctcta taggagaaag aagttttaca cttttcaaca atacgtttct
    13501 aattttgcta gctttgatct gtatcgttcc ttttttaaat atcatcgcta catcttttgc
    13561 ttctacacaa gaagtcgtcg cgaaaaagtt tatccttttt cctactacct tttctttaga
    13621 cgcgtatcgc tacattcttt ctacgccgac catttttaga gcacttgctg tttcgattgg
    13681 tgttacaggt gtcggaacga tcgtcagtat gtgtgcaact tcactgatgg cctacggtct
    13741 ttccagaaag tatttgtttg gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt
    13801 gttcagcggg gggatgattc caacgttctt agttgttcgt tctctagggc tggtcaattc
    13861 ttactgggcc atgatcctgc ctgtggctgt taatgcaatg aatatgatca ttatgagaaa
    13921 ctttttccaa gccttgccgg atagtttgga agaatcagca aaaatggatg gttgcacaga
    13981 ttttggtgta tttttcaaaa ttatgctgcc attagcgtta ccgtctattg ctactatctc
    14041 gcttttttat gctgttactt actggaacac ttatatgact gctattttgt atatcaacga
    14101 ttcatctaaa tggccgatac agattctttt acgccaaatc gtcattgtat caagtgggat
    14161 gcaagcagaa agtagtgccg ttgatgtgat tccaccagca caaacagtta aaatggctgt
    14221 tatcgttatt gcaacagtgc ctatgctgat tgtttatcct tttgttcaga aatattttgt
    14281 caaaggagca ctcgtaggtt ctgtcaaagg gtgattatgt actgaacgca aaatggtagt
    14341 tgtgtcagaa gtggtcagct tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa
    14401 tttttgtgaa atttcggctt atttttcgaa gaatctactc ctggaacacc gtgtatcaga
    14461 ggttgtgaaa gagacgttca gccttgagcg atgataaaaa ctctcacaaa cattgcgacg
    14521 agtaacccac aggagcaata agaagcgatt ttgaaaaata gcttcccata tttcacaaaa
    14581 ttatgcctta ttgtcgatag cctgtctctt gaacgccatt tattcgtaaa aaagaactgt
    14641 gacaagactt ttgtcacagt tctttttgcg tgcttagcct tgaaatttaa ctatccataa
    14701 atacaaatac tagggttatt ctaacttctt ttattgcttg aatcagtcca caagaaaaac
    14761 agcctcttga ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt
    14821 ttaaagaaac gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt
    14881 acggagaggt tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca
    14941 aaatcaccaa atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta
    15001 taatgcaacc tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg
    15061 atatgcataa tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat
    15121 tctgtgattg gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa
    15181 ttgttcagtg cttttacata aatttgtttc atgttatcaa agttttttga atctactccc
    15241 aataactgtc ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc
    15301 aaaggatttt tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact
    15361 tcctgatttc cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta
    15421 ataagtgttt tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga
    15481 ccagtattta cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca
    15541 ttcagcgttt cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt
    15601 acacgttttt gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct
    15661 ttatccattc gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga
    15721 ttttcctttg gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc
    15781 tccccaccgt gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca
    15841 ttttgagaac tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga
    15901 tttctaaata aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt
    15961 gcacttaacg cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt
    16021 tctttcccac tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga
    16081 cgggcaaatc tgtccgaaaa taagtaggaa agaagaccaa tgagtcctac cagtgttaaa
    16141 gacactgcaa ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca
    16201 acttctaatc tgtaaatcca attattataa tctgatttca gtactatata cgttttatct
    16261 ttttctctta tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt
    16321 tgctctttat tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa
    16381 agtatgtctt ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca
    16441 attcccaatg catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct
    16501 ttgtttttct tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt
    16561 tcaatttcct cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc
    16621 aaactgacta aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct
    16681 ttcctgattt cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc
    16741 gagaaatttt tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat
    16801 tcaacgttag ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg
    16861 atcgattcat aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct
    16921 aataaaaaca tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc
    16981 tcctcctttt aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa
    17041 tgagcattcc ccgctttcaa gaatagcata attaaaaata aaaatccaag taatttttaa
    17101 cgtgtgcaat cttttcatat caaagatcta ttgacttatc gtagttatcg taatatgatg
    17161 aatgcgttaa caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg
    17221 tcgctaaagc agctggcgtt tctcatacga cagtttctcg tgctttgaat aacagcagtt
    17281 taataaaaaa tgaaacaaaa caaaaaatac gacaactagc taaggaaatg aattattcgc
    17341 caaactatag tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga ctatttttct
    17401 caagtttgca taaaggaact tcatcaaatt tcctagcaga ttccattgca ggcatccatt
    17461 cagtattaga cacaaattac agtttatctg ttgaaagtat cgatgttgtt gatttaacag
    17521 aaatcaacct gcaacgctac gatggactga tcgttatgag tcaaagtgat gatgatcagc
    17581 ctttcatcga ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca
    17641 atgatccagc ggttatcaac gtcactgccg atgatgcggc cggtgttatg caggcagtag
    17701 attacggtat tggatgcgga catacgaaga tcgcttatat tggcggaaag aaaaattttc
    17761 gttcgacaaa agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg
    17821 tcaatccagc ctatttttta acaggagatt acagcttaga aagcggcctc caaaacatgg
    17881 aatacctatt atcactttct gacctgccta ctctggtttt ttgcggcaac gacgacatag
    17941 cgattggtgc attgagagct gtacatttag ctggcctccg tgttccccag aacatttctc
    18001 tgatcgggtt cgatgattct ccagtcgtct cctatctcaa tccgccgctt accaccgtac
    18061 gtaagccttt gaaagaaatg tgtcaagaag gaacaaagct tttactagat ttgatcgaag
    18121 gcaggacgat cgaacaatct aaaattgagc tctccacctt gttgatcctt cgtgagtcga
    18181 tcaattactt gtaaactaca agtaatttta tataaaaatg ttaacgtgtg cataacacag
    18241 aaaggaagtt tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg aagcacctgt
    18301 aaaagttctt cagtttggtg aaggcaattt tatgcgtgga tttattgatt ggcagattca
    18361 gcagctgaat aaccaacaat tgtttaaagg taatgttgca atcattcaac ctttagaaca
    18421 aggtcttgga aatatgatga accagcaaga taatttatat acggtcattt tacaaggact
    18481 cagcaatggc gaaatcgttg atacttccga gattatcacc gtagtagaac aaatgatcaa
    18541 tccttatgat gaatgggatg cttatctatc tctagcagaa aatgacgact tggcctatat
    18601 cgtctccaat acaacagagg caggaatcca attcgtggag actgataatt taacagatac
    18661 tcccccacag agcctcccgg gcaaactgac agccttttta taccgacgtt tcaaattaaa
    18721 caaagctggc tttaccatca ttccttgcga actgattgac agaaacggcg aacgtctaaa
    18781 agaaatcatt ttacgctacg cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt
    18841 agaaaaagaa aatgttttct gttgtagttt agttgatcgc atcgtcccag gttatcctcg
    18901 cgaagatgca ggtgttttca atcaacggca taactatgaa gatcaattga tggtcaaagc
    18961 agaaccattc atgctttggg tgatcgaagg gcctaaagaa ttggagaatc aactaccatt
    19021 caaacaggct ggcttgaatg tcatcatcac agatgatatg actccttatc gtgaacgtaa
    19081 ggtgcatttg cttaatggac cgcatacagc gatggtccct cttgctttgt tggcggaatt
    19141 ggaaactgta gaagaagtaa tgaaagatcc tttattctcc gcatatgttg atcagttatt
    19201 caatctagaa ttgattccta tgctttcttt acctaaagat gaattagcca tttatgctga
    19261 ccaaatcaaa gaacgtttct tgaatccttt tgcccatcac aaattagaag cgatttcatt
    19321 aaatagtgtt tcaaagttca gcacccgtct gcttccagtg ttcaagaagt atatcgaaga
    19381 acaaaatcag gtaccaccgt taatcactgt ttcactagct gcgctacttc ttatgtatcg
    19441 aggcgatcaa gtaaagccac atgatgatga aaagacgata agcgaattta ctgatgcttg
    19501 gtctgataat ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga
    19561 tttgtctcaa ataccgaatg tgacagatac agttcaagaa atagctgcaa agatcgatca
    19621 aaaaggtgtc cggtcagtat tacaagaaat gaaaggaacg atggtccatg cttgaaaccc
    19681 tgcaaccaac aatgaaatta aatcgacgag acagtgtggt cgtcgcatta acccctattc
    19741 caaaacaaac acaactgacg attgatcagc aaacgatcac cactcttgaa gatatcccgc
    19801 aaggtcataa gattgcattg ggtgatttga aagctggaga caacgtgatc aaatatggct
    19861 atccaatcgg tcatgtgacg acagacgtaa ctgccggtca atggctgcac actcataatg
    19921 taaaaaccaa tctttctggt gaattggatt atcgatatga aaaagatgtc catccaagtc
    19981 attacctttt tgagaaccga acatttcaag gttacttgcg aaaaaatgga aaagtaggga
    20041 ttcgtaatga tttgttttta gtacctaccg ttgggtgtgt gaatggcatt gctgaactga
    20101 tcgtcaaaca gttcaaggaa aaacatccag atctgggaca gtttgaccat attacgattt
    20161 taaagcatcc ttatggctgt tcgcagcttg gaaaagatca tcaaaatact cgagaaattt
    20221 tagcggacgc agtaaatcat cccaatgccg gaggagtttt agtatttgga ttaggttgtg
    20281 agaacaatac agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga
    20341 aatttctagt ggctcaagat gtttacgatg agatcgaaca aggtgtggct ctattagaag
    20401 agcttttaac agcggcggaa aatgaccagc gcatatcagt cccgctctct aagttgaatg
    20461 tcggtttgaa atgcggcggt tctgatggtc tatcgggaat aactgctaat cctttacttg
    20521 gtgcattttc tgactacttg attgctcaag gaggcagcac gatcttaaca gaagtgccag
    20581 aaatgtttgg cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa gtttttgact
    20641 caatcgttga cttgatcaat gacttcaaac agtatttctt gtcttacggg gaaccagttt
    20701 acgaaaaccc atctcctggt aataaagcag gtgggatcac tacattagaa gataagtctt
    20761 taggctgcac gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa
    20821 aaatcagaaa accagggctt agtttattgc aagctcccgg gaatgatctt gtagctgcct
    20881 ctgctcttgc ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt
    20941 ttggctcata tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag
    21001 gtcattggat ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag
    21061 aatcgtttgt tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa
    21121 atgacgttcg agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca
    21181 gtataaaaag aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga
    21241 ttgggcgaaa aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg
    21301 tcacttagat ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg
    21361 gttaggaggc gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa
    21421 aatcacagga aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa
    21481 aattatcgga aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat
    21541 tgacttgttg ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt
    21601 ggcagctcct gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg
    21661 tacaacagat gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc
    21721 agatttgaag gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg
    21781 atttaatgca tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca
    21841 agaactgata gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt
    21901 ttccgaccac gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga
    21961 gatttttcaa aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatcg
    22021 tacagaaaca ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct
    22081 gcacatccat gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac
    22141 cggctatgat ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg
    22201 tgcagaagaa acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta
    22261 tcctgcgatt ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt
    22321 aggttctgga tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt
    22381 tgctgatggt agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct
    22441 gtcctacact agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt
    22501 catgcgagga gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag
    22561 ttatacaaat gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa
    22621 cgaatcaaaa acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag
    22681 acggaccaaa tttgctccgt cttcttctct tgctgttctg cttcattgcc tataaacctt
    22741 tatgaaagaa gagataacat gcctactatc catattgccg gtgattctac tgcggcagaa
    22801 aaagatgaaa acaagcgtcc cgagacaggt tggggagaaa aaatctctgc ttatttttca
    22861 cccgagatcc agatcaataa ccaagcaaag aatggacgca gcaccaaatc atttatccag
    22921 gaaggaaggt tagcccgtct agcttcacag tttcagccga atgatttctt atttgtacaa
    22981 tttggacata acgaccagaa aatccaagaa gaaagaggta cgcttccctt cggtagctat
    23041 ttaaaaaatc tgcagatatt ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta
    23101 ctgacatctg tgacgcgacg agattactta gaaaacggaa cattgaatcc agatatactt
    23161 ggagactatc caaaagcgat gcgtgcattt gctgagaaac accatattcc tcttttagat
    23221 gtgttctcaa gatcacaaga attgttccag accttctcaa aagaagagac aaaaaaattc
    23281 tacctgcatc ttatacctga tacttgtaaa aattacccag aaggactaaa agacaatacc
    23341 cactttagtc cagaaggcgc tgataaagtt gcccgaataa ttgttgaatt gataaaagaa
    23401 tcccgtctgc ctcttgctaa ttatttacaa aaaggagttt gagatgtttc cattaaattt
    23461 aggcatccgt gcccacgatt tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc
    23521 acagcttcac ttgtcccata tccaatttgc accacaaaaa gcatttcctg aacaacttaa
    23581 attaactgac atgacacttg ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa
    23641 tatcgaactt tccattttag gatgctatat caacctatcc agcaaaaatc aagaagttcg
    23701 tgcacaagca ttggcaactt tcaaacagca aattgctctg tgctctgcct atcaagccaa
    23761 gatgattgct acggaaaccg gtagtgtctc tgtcggttac acaaaagaaa attttactga
    23821 agaagcttat cagatcgcca gaaattctat cattgaatta gtcgctcatg cagaaaattt
    23881 tggcgtaact gttgctgttg aagcagggat caatcatcca atctataact atcagttagc
    23941 taaacgttta atcgatgaag tagcttcacc aaatttaaaa atcattttag attgtgcgaa
    24001 tttgatgcat aaagaaaatc atgctgatca ggaaaaagtg atccgaggtg ccttggacaa
    24061 tttacatggt tatattacag cgcttcatct caaagactat atcatggaaa atggaaagat
    24121 ccgcatggta cctgttggca agggctgttt agatttccgc ccggtactgc attatatcaa
    24181 atatgagcgt cctctaatgt acgccactct tgaagctact cctgagattt atgttcctga
    24241 agcaatagaa cacttgcagt ttatttacca agattgttaa ggagaagatt atggaggaac
    24301 gaatacagaa aagttatcaa aaatatcatg caaatgcttt tcagacattg ctgcatcttt
    24361 atcagggaaa ttattggcgt tttgccatgt caggactctt ttttattatc aaacatcttc
    24421 catcttggtt aatgccgatc gctattgcca atgtaatcaa tgcagcttca tcgaaagatt
    24481 cttctcagtt gcaggccatt tatttcaacg cttttttgat ggcttgtctg attggtcaaa
    24541 atatattgac gaattatttt catgtaaaat accatagcac ttcgatccgt caagtagaag
    24601 ctggactaag agcgaccctt atcaagaaaa tccaagaact atctattcct tatcaaaaag
    24661 aattgcaatc tggacgaatc cagtcaaaga tcatccgtga tgtagaagct atccaaacgt
    24721 tgtcctctca agtatttgtc agcggattaa atatcgctgt caatttattc gtagctttag
    24781 gtatcacgct ttataaaagt cctgttgttt tcatgttttt cttattgact gttcctctcg
    24841 ccgcattcgt tactctcttt ttccgtaaaa ggatcagaca gtccaactca gacttcaggc
    24901 aagaaatgga agagacttct gcaaaagtga tcgaagtgat cgaattagca ccgattgctc
    24961 gagctcatgg gttagaaaaa cacgaaacaa ctcgcttaaa taagcatttt actcaaatct
    25021 atgaaaaagg attccactta gatatcctgc aatctgtttt cggatcagtc agctgggcta
    25081 gttttcagat atttcagctg atttgtttga ttttcactgg gatattagct ttacaagggc
    25141 gcatccaacc cggcgatgtc gtgatgtatc agacgtattt cacgacgatc gtcaattccg
    25201 tttctggaat gatcacgctg attcctacga tttcaaaagg catggaatct atcacttcta
    25261 ttggcgaact gttgattgct ggcgatgtag aaacttatca cggtaagaaa aaaatccaac
    25321 atttggatgg aaattttcgt tttgaggatg tttcttttct ttatccggac tcactcaaac
    25381 cgattatcga tcatttttct ctagaggtga aagctggaga aacaattgct tttgttgggc
    25441 catctggctc tggaaaatct acactcctta acttattgat tggatttatc cagccgactt
    25501 caggaaaaat ttttctagat gatcagcctt tggatgaact ggatatgcga agtgtgcgaa
    25561 attatttagc cgttgttcct caaaccactt tgttgttctc cgcttcgatc aaagagaaca
    25621 tcacttatgg cttgaaaaac gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc
    25681 aactttcttc actgattgac cagcttcctg atggtctgga taccttagta ggcgaacatg
    25741 gcaacaagct gtctggtggc caaaagcaac gtatctccat tgcacgagcg ttgatacgtc
    25801 agccaaaaat cattttgcta gatgaagcaa cctcagcttt agataaccag tcagagaaaa
    25861 aaatccagca agccctgaat tacctgacag aaacacccac tacttttatt gtggctcatc
    25921 ggctttcaac gatcaaagag gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg
    25981 aaataggtac ttacgaaaaa ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg
    26041 aatgacaaaa aataggagct atgacaagac ttttgtcgta gctcctattt ctgaataaac
    26101 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg
    26161 ttttttgaaa ttgttccttg taactcagag ttaaacggta ggctcacgac ctcttggtaa
    26221 atggtgttca agagacagcc tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg
    26281 aacgtctctt tcacaccctc tgttgtttca taaaatagtt ttattcgctg taaaggtcgc
    26341 gaactggtgt catgatgata cggttcaagt cattgatgat tgtactgaag ccttgctctt
    26401 tttgcatcaa gtcattgatt acttcttctg attggatttt cacagccatt tcttgtgctt
    26461 tcattgcatc ttcgtcgctg aactcttgtc cactcatttg tttttcttgt aattcgattt
    26521 gcatcgcttg gaagtctttg aacagtttgt gtgcttcttc gtttgcttgt actttctcgt
    26581 acgcttcttt caacgctttg aattctggta attcacggat ctcacgttcg atttgattcg
    26641 cgctatcgta gatgtttgac atgttatgcc ctcttttctt ttagtagtta ctctctattg
    26701 taacatgggg ttattcgttt tcaaaggggc tactctcctt gatatttccg ccagaggtct
    26761 ttagccgctt caccaattcc ctgaacgcct tctttcacca tatcgccgaa gtttttcagc
    26821 ccttcttttg cttgttcacc atattgattc aagttatctt gccaatttcc agactctcca
    26881 ttagaatttc ctgtgtttcc aacttgatct gcagctacca cttgtcctcc tgtggcatag
    26941 gcatctgcta cactaaatcc tgtttttgaa gtatacggaa ggatgccttc tgccgctgat
    27001 ttgaagactt tcccgacaac atttccactt gtcccttcca aatagtgcag cttgctggat
    27061 tcttcgtaac ccatccatgt cgaaatcact acgtctggcg tgtaaccgat catccattgg
    27121 tcatttgctt ttgtggcatc gaaattagtt tctgtggttc ctgttttccc tgctatcgta
    27181 taacctgcag gattcgcagc agccgctgtt ccatttgaaa atgtaccaag aagcatgctg
    27241 gtcatttcat cggctgtttc ctttgatatg acctgttttg gttttttgtt tgtgttatcg
    27301 acgatcaccg ctccagtcga atcaacgatt ttcgtgataa gatgcggcgt atacatctga
    27361 ccatcattgg caaatgcact gtaagcaccc gccatgatca atggagatac tcctttttcc
    27421 agtccaccaa gggctaagcc ataatactta tcagattttg ctaaaggcag gccgaattct
    27481 tctgctttat catatccttt ttgtaaaccg atctcatgca aaagccatac agtaggcaaa
    27541 ttcaaacttt gtgcaacagc ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa
    27601 ttatgcgcat cgtaataact ttgcggttcg tctttcaaga tactggaagg cttgtatcca
    27661 gcttccagtg ctggtgtata tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc
    27721 atctgtgtag caaagctgaa tcctctaaag acatgatctc ctcttcgtcc tactagtgct
    27781 tgtacaccac ctgtattcgg atctaaagct actgaagcag attggaccat ggcaccatct
    27841 gaagcatttg ctgggaataa tgcactgttt tgataagtag cttctaattg cttttgataa
    27901 tcctgatcta aagctgtata gattttatag cctttattca tgacatcttc ttcatcaaga
    27961 ccatactcgt taactgcttc atcgatcact gcatcaaaat aataaggata acgataacca
    28021 gaatctgaat tatcgtatgt gtcattaaga tacgcgctga tatctgtctg gctttctgct
    28081 tgttcttgtt cttttgtgat ttttccgttt tcttccatta cacttagtac tgtgtttttc
    28141 cgatcattcg cattttttgg gtgatcgatc ggattataaa taccaggacc tttcagcatt
    28201 cctgccaaag tagcagcctc agacagcgta acattcgttg catctactcc aaaatacttt
    28261 ctggaagcgt cttggacacc ccataccccg ttgccaaaat aggcattgtt aagatacatg
    28321 gtcaagattt cttctttgct gtacttcttt tcgatctcga ttgccaaaaa taattccttc
    28381 gcttttcgat caaacgtttg gtctaatgtc agatacgcat ttttggcaag ctgctgagtg
    28441 atcgtacttc ctccgccccc tgatgttccg ccagacaaga ccattcggac tgctgcacgg
    28501 gcaatccctt tgatatcaaa cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt
    28561 actgcatctt ggatataggg agaaatcgcg tttaattcta catatgttcc tttttgggcg
    28621 tacagtgtgc cagcttcttc gtttttctta tcataaatga ctgtcgattg gcttaatcct
    28681 gattttaacg tttcgacatt tgcttgtttt gctaaaataa agaggtagat acttgtcact
    28741 aagacacaga tcaaaccaat cagcaaaatc aatttgttca catgatattt tttccagatg
    28801 cgctttcgcc atcgatggaa ttgtccgaga tatggtctga tccaacgcca aaaactgatc
    28861 aaagcagctt ttatcttttc taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta
    28921 tttgattcaa tcgtcttatt ttaccatagt gaaagagtgt tttcttcctg cttaagttgt
    28981 agttcaatat gtttttttac gttagtgcaa gtagagtaac ataagaatga acagaaaaat
    29041 gtaagttttt tcttaaatat gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa
    29101 ggagtacact tgtgaaaatc acgatcactt tacctatgaa ccatcctgaa acaacaattc
    29161 gcgacttgct tgaaaaggaa tggttagttc ctagaaaggt tcgacacttt ttgcgtatac
    29221 gtaaaaacgt ttggatcaat caacaacctg ctttgttcca tcatgaagta aagggcaatg
    29281 accagatcac attgttattc gaagaaacag attataatta tcaagaagtt caattaggag
    29341 atgcgaagca catccgtgtt ttatatgaag atgagcattt gattatagtg gataagcctg
    29401 caggaatgaa gactcatcca aatgaaccga cagaaaacag taccttactc aatcatttag
    29461 ctgcttatct cgacactaaa cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa
    29521 caagcggtgc cgttttattt gcaaaaaatc catttgtctt gccgatactt ggaagaatgt
    29581 tggaacaaaa aatgatctat cggagatatc aagccatcgt atggggagaa attgccaaag
    29641 atatgaccat caccaataaa attggacgag atcgccatga tcggagaaaa agggtcgtag
    29701 atgataaaaa tggtcagagc gccttgacac atgtcactgt caacaaagta ctttctgaac
    29761 aaacacaagt ctattgtgtt ttggaaaccg gacgtacaca ccagatcaga gttcatttag
    29821 ctcataaggg acatccgatc gttggagacc ctctctacca aaatagaaca gctgaacgac
    29881 tgatgctcca tgcattagaa ctgcacatga tccatccatt tactcaagaa aaaatagtcg
    29941 gtaaagcttt acctggatta tgggaataga aataaaaaag aacagagtct tatcagcaga
    30001 taatgataag actctgttct ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc
    30061 tgctaacagc aaactaccga taatcccact ttcattcggc agtccccaac gaacgatata
    30121 ttcacttact tgaggtgttt ccatataacc acccatcaat tcaacaaatt gctgacggat
    30181 tttttgaagg agatgatcct gattcattac cccaccgcct aaaataatct tttcaggtgc
    30241 taacgtcaac gtatagttga tcagtgcttg agcaatataa taagcctgaa tagcccaaac
    30301 aggatgatct tgcggcagat cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc
    30361 tgctgctaat ccttctaaac aatctttatg ataaggacaa gtaccttcat aatggtcatc
    30421 tggatggcgt ttcacccaaa tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc
    30481 ttcattgatc acgacacctg caccgattcc tgttcccacg gtaaggtaaa tacaactatt
    30541 ttttccttgt gctgctcctt gcttcaattc cccatatgca gcagcattga catccgttgt
    30601 ccaagcagca ggaatattgt agcgttcctt gattgtgcca agaaaatcga attgtgccca
    30661 cccttttttg ggagttgcca aaacgtatcc gtaattatct tttgttttgt cgataccaat
    30721 cgggccgaat gagccaatcc cgattccctt caattcataa cgatcaaaaa attcaaatac
    30781 ctgatccaat gtttcttctg gtgttgtggt agggatactt aatttttcta aaatttctcc
    30841 accatctgaa accgcacaca caaattttgt tccgcctgct tctattccgc catacattcg
    30901 ttttcctcct cgaatccgat tgtccgaatc tcataaggtt ggattattgg gacaaccgct
    30961 tgcttcgttg gttcttcaag cagattcaat atgacagttt ttccattgtc tttagttatt
    31021 tccaattttt ctaaatgact agacatattg aaacctctta ctactacttt atcactaaac
    31081 ttgctgcgtt tcaaagctgt aattgcaaac gtttcacttt ttatcgttaa atattgttgt
    31141 tttgaaatca atgttccttc atgatgtttg atttgttgag tggaaaaagg aatctgtgct
    31201 gcgtatgcat gcaaataagt agagaatttt tcttctggtc catgtagttc aatgctgtaa
    31261 ttgaaccgat gctcacctag acactgtgct tctggtgttg gaaaatatcc ccaatcgcct
    31321 agttctccca cactgcgaag tagcgtcact gcgatttgtc cgtcttctgt tacttcatat
    31381 tcattcaaac caaagtttcc gactgttaca ccgtactctt catcatgaat attgacaaat
    31441 gcttgctgat gttgcggatt cgtggggttt tcccaacttt tagacacatg gtttgggcgc
    31501 ttcaccactt caaaaatact atctgcttcg tgatgttcca catgtaactt tgtaggaaac
    31561 aagacacgta agcgatgatc tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt
    31621 ttactatctt ttctgattgt catgattgtt tttatttgaa ggattcgttt ttctttggat
    31681 cgttctgctt ttcgataacg aaattccaca accatctgct gttctttttc taaacgctcg
    31741 tctgcagaaa cagggatttc catttcctgt agaatactta tttttgctat ttccggtgta
    31801 tctactaata cttttacttc agagttttct ttgttactgg ataggattgg ctgatcacag
    31861 aaaggttgtt taaagatata ctcatttcct atatcgccga tatcttcaaa aatatgcaga
    31921 ttattcaact ttcgtttgtt tttgcgatct tccattgtaa tggtaccatt tttttcaatc
    31981 gtcaaatgta gagggccgtt ttcgatgatt cgtccactct gatgaatcat tgtttccttt
    32041 tcttctgctt tcgcttcccc ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg
    32101 ttttctaatg gtaaagtcac tttgatcatt cttgccatat atggcacacg gaaacggtct
    32161 ttcggcaaat cataaccaaa ttggacagtt tcttcagata aaatagctgg tatagtggcc
    32221 ccactttttg tttctacatg atacttacgt ttaggcaaat tttctaactc ttgatatagt
    32281 tgttcaggta ttccttcagc gaatcttttt ctttctaatt cgatggttat ttctgcttca
    32341 ccagttttat ccattcctgc agtatttaca atgacaaatg gaaagctgtc ttttgggaat
    32401 ccggtcgtat cgattgctgc ggtcagctgt tctaaggaat cttgtgcaag atactttcca
    32461 acttcatctg ctttttcaaa acgaggtatc atttcccgat ggacagaatc gactgaacaa
    32521 ccgcaaatac tgtcgtgagg atgattttgc attaatgtct tccaagcata atctaattga
    32581 tcatggggat agtttccact aacttcataa gccatcgtcg caagtggttc tgtaatattt
    32641 tctagctgtc gctggacctt ggtattccat tgttttaagt aaacccgagc agatgctgta
    32701 ttggctaaag tataccagcc atccgtttct tgacttgtca attctccttc tactgagcct
    32761 aagtcttctg gcacatcttt ttgaactgct tccagatagt cagtaaaatt agaatgaata
    32821 aactcgtaat ctggaaacag ttcatttgcg agatggattg cttttgatat atctttttgt
    32881 accggttgat gatcaacacc attcatcatc aataagtggt tcgtagaggc atattgttct
    32941 gcatccgcta atttttgctt ccaaaaggca agagctgctt ctttttctac tggaatctca
    33001 ttcccgttgc tataccaatt ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct
    33061 ttccaccaca tctctgagta ttgggaagaa tagttttctg cttccaaaac ttgattatca
    33121 aagccaattg gttttacacc acggccaaac gctgctgctg aaataccagc ttgtttcatt
    33181 aattgaggcg tctgtcccat atttccaaat gtatcgggaa aatagcctaa catgacaggg
    33241 gttccccatt tacggctttc ttccatgcca atcagcatat ttctcacgtt cgattccgag
    33301 ctgataagaa aatcatcttg caagatataa aatggtccaa ttctcaattt tccttcattg
    33361 attgcttgtt ggattgcctg tcttttttct ggccttactt gaagatagtc atctaaaata
    33421 atcgtttgcc catccaaatg aaaactgttg aaggacggat caacttcaaa tagctctagt
    33481 agatcgtcta tcaattcaat cagtcgcatg tgatgctgtt cgtaagccat gtaccattca
    33541 cgatcccagt ggctatgaga aatgatatat actttctttt tcatttacga tacctcctac
    33601 ttttctactc taatatcaaa gtaatccatg actaattcgc aaaacatcat atttgcccaa
    33661 gaaaaccatt ctctcgtata attttcagga ttgttcacat cgaatccttc gtgcataagg
    33721 tgtgttcctg cgtcagttgc tacaagtaaa ttcaatatcc gctctttttc agatttgtct
    33781 gacgttgtca ttccttccat agctaatgca atcggccaaa cgtatttttc tggcgtatgg
    33841 gaggatccaa ttccttttgc gtactttcct tcatagaaat atggattttc tttactcaac
    33901 aatgttcttc ttgtttgcag ataatcttcc tcttgttcag aacaatatcc caagtaaggt
    33961 gcggcgatca gattgggcac attactgtca tccatgactg tggcgtttcc cattccatct
    34021 acttcatatg cataaatcgt ttgccctttt tgattaaccg ttcgtccgtg attctgaatc
    34081 ccctcttgga tctctttctt caatttctgt gtttgctgta caatttcttt atcatcgagt
    34141 atcgtttgaa aaatttcttc cacataacct aacacgacca ccgcaaacat attggatggt
    34201 accagatatc cataacggca agtatcatcg ctaggacgga aaccagacca tgtcattcct
    34261 gtaaaagctg tttttgcacc tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg
    34321 tctcgtacaa atgtataagg agagtgttca tgatcttgct ctgttttgaa cacgtccaag
    34381 atttttttga taccttcaat aaaagattga ttgaattgat cagtttttcc tgtatttttg
    34441 taaagaagat aggccaactg tattgggtag cataaagaat caatttcata ttttctttcc
    34501 cagatccaat cgttcatttt tgtatgatct gtttggtggc cagcaccgtt agcttcttcg
    34561 ttgaaggcat tcgcatatgg atcgatattg atataaaaaa attgcttttt gactaacccg
    34621 ctgatcatgt cagctaattc ctcatcttct tttgcaatca ctaaataagg acgaacttgt
    34681 gcagtagagt ctcttaacca catcgctgga atgtctcccg tcaataaaaa ggtggttccg
    34741 tcattttgct ttttgactgt tgtcaacaga gtattggcaa atgctgctgt aaaattcttt
    34801 gcccagtccg catggatatc cccacatttt tgtgtgatat cttccataaa tttctgcacc
    34861 gattttggta tatctttata agccactaat aaaaccttct ttctatttga tatatcttta
    34921 aagattatac ttcatacagc caattatgtc tacgctttca aaaaatatct ttttatttac
    34981 tttttcatcc attatggtat tatttgaata tgatatatca ttaagaggtg tttatatgaa
    35041 tcaaccattg taccagacaa tatttaatga tttgcaaacg gctattttaa ctggtgaatt
    35101 acctgtagac agccaactac caactgaaaa agaactttca cgtacctata aagtaagcag
    35161 aatcacttcc aaacgtgcat tgactgaatt agagcaatta ggacttattt atcgcgttcg
    35221 cgggaaaggc agtttcgtta aatccgcttc tccggaacag attcttgctt ctcctacaaa
    35281 aacacgacgg attctgtttc ttcttccata tgtcgctgat ttgtctgttg gtgattttag
    35341 caagggattg aaccctgtga tgcaagataa acagattgat gtcatgatga cgacgcttga
    35401 ctttctacaa aataaaacag caaaagagct aatcaatgaa tttgacggat tgatttatta
    35461 tgctcttgat acagaacaac atcttgactt actttttgag ctttcattga aagaattccc
    35521 tgtcgtcata ttagataaaa aaatctatga gttacctttt cctaccgttc tttccgataa
    35581 tttccaaggc ggtgcgcttg ctgcctctta tttgatcgaa cagggacatt caaaaatcgc
    35641 ttatttgttt ggtacgcaga ctcatccaca atccgttcga cagcgttacc ttggttacct
    35701 tgaagcattg gacaaagctg gtttgacttt ccacacttca ctggatgaca aaagcgcgac
    35761 attagatgaa ttgctttctt atatcactaa gtatgaagta actgcttttg tttgtgaaaa
    35821 cgatgttgtc gctatccaga ccatgaatct cttgagacag catggttatc atatccctga
    35881 agatttttca attatcggat ttgatgatat ccaagcagca gctcttgttg acccgccact
    35941 gacaaccatc gcacaagatt ttgaacagtt aggtaagctt gcgggagatg ctttggtcac
    36001 ttggttagag acaaaagatc gaccatccga tagtaaattt ccagtttcat tcatcaaaag
    36061 acaatcaaca aaggagaaat aaaatgaata tcggtcaaat agatactaga catgcaacag
    36121 cgaatcagcc aaatttctct aatgggaact gccagcctta tactggcgtt ccttttggga
    36181 tgaattattt tgcaccgcaa actacggatc aaaacggcag ctggtggttt catcctgatg
    36241 accgcgtttt tcaaggctac cgtcttaccc atcaaccaag tccttggatg ggtgatttca
    36301 gtcatatgct gtttacgccg atcaatggaa aactacagga gaacaccctt ttccatgctc
    36361 aaagttctta ccgcccagaa gaaagtatat tttgcccgac tcatctgtcg atctgtcaat
    36421 tacgttatgg catctgttct acattgatac ctagcatgta tggcggaatc ttatctattg
    36481 attacagcaa aaatgacagc ggtttacttt tatcctttcc gggacgccat cagctctttg
    36541 ttatagatcc ttacacagtg gaaggaaaaa tcacacattt tgctggttca gaagatcctg
    36601 atttttcttt ttattttata ttgagatttg aatatccttt aacaattgat gagttagctg
    36661 tttccaacaa aaatagcgac tcgatcccgt tgtattttga acagacaaaa aaacaaacta
    36721 tccgatttgg gacatctttt atcagctcgg aacaagcagc ttataacctg gaactagaac
    36781 aaaaccaaag caaagagact taccttgctc acagtcaaga acaatggcag tcctattttg
    36841 atcgtataca gctcacacac catgatcaac aacaattgcg gacgttttat cataatttat
    36901 atcgggtgtt tttatttcca caaacatttt atgaaattga tcctagtggg caaaaaatcc
    36961 actatgatac gactagtcga tctataaaac ctggtgttct ctatacaaat aatgggtttt
    37021 gggatacgta taaaacggtt tatcctcttt attctttgat tgctgtagaa aaatacgaag
    37081 aaatgctgga aggttttttg aacagctata gagaaagtgg ctatttgcca aaatggctgt
    37141 ctcctgatga acgtggactt atgcctggga cattgatcga tgcggtcatt gcagatgctg
    37201 ctgtaaaaga tatccgacga gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg
    37261 ctacggttca aagcaaacat ccaaattatg gacgccaagg aacagaagat tacttgacat
    37321 atggctatgt tcctaatcac taccacgaat cagtcaatca cacattagac tatgcttata
    37381 gcgatttttg tattagccaa gttgccaagg tactagaaga agaggaaatg acgaagaaat
    37441 atcagcagca atcattgaac tatcaaaata tctttgacta tgagtcagga tttatgcgtt
    37501 caaaagataa agatggcaag ttcagagaac cattttcagc taatcgttgg ggacaagatt
    37561 atgcagaagg aagtgcttgg caaagcagct ttgctgttta tcacgatttt tcaggactga
    37621 tcggaaaata tggtggcagt gagagatata aagaaaaact cattgagctg tgcaatcagc
    37681 ccccaacgtt tgacccagga ggatatggtt ttgagatcca cgaaatgagt gagatggccg
    37741 cgatagaatt tggacaatta gctatttcaa accagccaag ttttcactat ccttacttgt
    37801 tcagttatat aggacaacct gaaatggcac agccactgct taaacagttg atgactcaga
    37861 ccttcgacga ttctccaatg ggttaccctg gagatgagga caatggaagt atggccgctt
    37921 ggtatatctt caatagtctg ggattttatc ctgtcacgcc tggaaccggt gaatatgtta
    37981 tcggtatgcc attaattgac aaggctgttt tgcttttgtc aaatggaaag gaattgacga
    38041 ttgaagcaac gcctaatcgg ccgcagcaac aatttatcca taaaatcaaa cggatggaca
    38101 aagaatatac gaaattatat tttacccatc aagatttgct gaaaggcggc acgatctctt
    38161 accaattagg gatcatccct caccctaaga aatatcaagc aaaagatttg cctttttcct
    38221 tatcaaacta taattaacaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
    38281 agccaagcaa aaatagatcc tcatattttt cgtttggttg gtcttattac cgcagatgca
    38341 agcttttgaa caccgtttat tgagaggttg tgaaatca
//
LOCUS       DACCQI010000007        37500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCQI010000007 DACCQI010000000
VERSION     DACCQI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.007"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..275
                     /locus_tag="IVO76_000294"
     CDS             <1..275
                     /locus_tag="IVO76_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP7428289.1"
                     /translation="LIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERINE
                     LIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            418..639
                     /locus_tag="IVO76_000295"
     CDS             418..639
                     /locus_tag="IVO76_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7428290.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            655..1497
                     /locus_tag="IVO76_000296"
     CDS             655..1497
                     /locus_tag="IVO76_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428291.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1560..1718
                     /locus_tag="IVO76_000297"
     CDS             1560..1718
                     /locus_tag="IVO76_000297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428292.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAENFRLSLKAP
                     EIRAFRRI"
     gene            1914..3113
                     /locus_tag="IVO76_000298"
     CDS             1914..3113
                     /locus_tag="IVO76_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7428293.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            3135..3347
                     /locus_tag="IVO76_000299"
     CDS             3135..3347
                     /locus_tag="IVO76_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7428294.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3422..3898
                     /locus_tag="IVO76_000300"
     CDS             3422..3898
                     /locus_tag="IVO76_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7428295.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            3895..5703
                     /locus_tag="IVO76_000301"
     CDS             3895..5703
                     /locus_tag="IVO76_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7428296.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            5780..5995
                     /locus_tag="IVO76_000302"
     CDS             5780..5995
                     /locus_tag="IVO76_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7428297.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            6087..6950
                     /locus_tag="IVO76_000303"
     CDS             6087..6950
                     /locus_tag="IVO76_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428298.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            6981..7523
                     /locus_tag="IVO76_000304"
     CDS             6981..7523
                     /locus_tag="IVO76_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7428299.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            7537..7959
                     /locus_tag="IVO76_000305"
     CDS             7537..7959
                     /locus_tag="IVO76_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7428300.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            7889..10288
                     /locus_tag="IVO76_000306"
     CDS             7889..10288
                     /locus_tag="IVO76_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7428301.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            10320..12311
                     /locus_tag="IVO76_000307"
     CDS             10320..12311
                     /locus_tag="IVO76_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7428302.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            12334..12585
                     /locus_tag="IVO76_000308"
     CDS             12334..12585
                     /locus_tag="IVO76_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7428303.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            12575..13804
                     /locus_tag="IVO76_000309"
     CDS             12575..13804
                     /locus_tag="IVO76_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7428304.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            13801..15882
                     /gene="topB"
                     /locus_tag="IVO76_000310"
     CDS             13801..15882
                     /gene="topB"
                     /locus_tag="IVO76_000310"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7428305.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            16031..19951
                     /locus_tag="IVO76_000311"
     CDS             16031..19951
                     /locus_tag="IVO76_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7428306.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            19952..20896
                     /locus_tag="IVO76_000312"
     CDS             19952..20896
                     /locus_tag="IVO76_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428307.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            20900..21103
                     /locus_tag="IVO76_000313"
     CDS             20900..21103
                     /locus_tag="IVO76_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428308.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(21403..21639)
                     /locus_tag="IVO76_000314"
     CDS             complement(21403..21639)
                     /locus_tag="IVO76_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7428309.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(21822..22193)
                     /locus_tag="IVO76_000315"
     CDS             complement(21822..22193)
                     /locus_tag="IVO76_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428310.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(22190..23518)
                     /locus_tag="IVO76_000316"
     CDS             complement(22190..23518)
                     /locus_tag="IVO76_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7428311.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(23479..23808)
                     /locus_tag="IVO76_000317"
     CDS             complement(23479..23808)
                     /locus_tag="IVO76_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7428312.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(24066..24437)
                     /locus_tag="IVO76_000318"
     CDS             complement(24066..24437)
                     /locus_tag="IVO76_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7428313.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            24582..24767
                     /locus_tag="IVO76_000319"
     CDS             24582..24767
                     /locus_tag="IVO76_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7428314.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            25710..26369
                     /locus_tag="IVO76_000320"
     CDS             25710..26369
                     /locus_tag="IVO76_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7428315.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            26372..27715
                     /gene="vanS"
                     /locus_tag="IVO76_000321"
     CDS             26372..27715
                     /gene="vanS"
                     /locus_tag="IVO76_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7428316.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RSDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            27886..28695
                     /gene="vanY"
                     /locus_tag="IVO76_000322"
     CDS             27886..28695
                     /gene="vanY"
                     /locus_tag="IVO76_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP7428317.1"
                     /translation="MEKKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGW
                     RLASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIP
                     AQYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKI
                     AEYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSY
                     RFGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            28713..29540
                     /gene="vanW"
                     /locus_tag="IVO76_000323"
     CDS             28713..29540
                     /gene="vanW"
                     /locus_tag="IVO76_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7428318.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            29537..30508
                     /locus_tag="IVO76_000324"
     CDS             29537..30508
                     /locus_tag="IVO76_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7428319.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            30501..31529
                     /gene="vanB"
                     /locus_tag="IVO76_000325"
     CDS             30501..31529
                     /gene="vanB"
                     /locus_tag="IVO76_000325"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7428320.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            31535..32143
                     /locus_tag="IVO76_000326"
     CDS             31535..32143
                     /locus_tag="IVO76_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7428321.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            32731..33162
                     /locus_tag="IVO76_000327"
     CDS             32731..33162
                     /locus_tag="IVO76_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7428322.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            33169..33402
                     /locus_tag="IVO76_000328"
     CDS             33169..33402
                     /locus_tag="IVO76_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7428323.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(33324..33566)
                     /locus_tag="IVO76_000329"
     CDS             complement(33324..33566)
                     /locus_tag="IVO76_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428324.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            33823..34026
                     /locus_tag="IVO76_000330"
     CDS             33823..34026
                     /locus_tag="IVO76_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7428325.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            34110..35303
                     /locus_tag="IVO76_000331"
     CDS             34110..35303
                     /locus_tag="IVO76_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7428326.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            35480..37183
                     /locus_tag="IVO76_000332"
                     /pseudo
     CDS             35480..37183
                     /locus_tag="IVO76_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
     gene            complement(37242..>37500)
                     /locus_tag="IVO76_000333"
     CDS             complement(37242..>37500)
                     /locus_tag="IVO76_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7428327.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         9908 a         9179 c         9961 g         8452 t
ORIGIN      
        1 aactcatcaa gctggctaat aagggtcaag attatgaaca tctagcagat gagattgatg
       61 agctgagaga caagcgacag ctccttttag tagaagatgc ctccctcagt ggcgagaacg
      121 agcgaatcaa tgagctgatt gaatttatcc gcaagaacaa attccgtacc ttagagtacg
      181 atgataagct tgtaaggaag ataatccaga gcgttacagt ctatgaagaa cacttcgtca
      241 tatccttcaa atctggcatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgat
      301 tctacagtag agtcgtgggt tttcttttgc ttgctgatta taataatatt ttaataacta
      361 ttgttattat aaaccaaatg atttataata ttcttagcgt aaacttggag gtgctgtatg
      421 actacggcag aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt
      481 gctagacgta ttggacagac tccacagaat ttcaataaga aattacaacg agaaacggta
      541 accttagatg agttgaaagc gattgctgat gtgctaggtg tcaagtttgt gcaggctttt
      601 atattgcctg atggcaatga aataaagata ggtaattagg aggtgtaatt tgtattgaaa
      661 atcataacaa taaatgatgt tgagtatgct gtatttgctg ctaatgaagg tacttccaaa
      721 ccacaacctc atatcataga aacgaaatct ggtactattc cagaaggaaa acaactgagt
      781 ttattaaaag aatatcttaa gcagaatgat atttccccaa taaaaggagc aacaacatat
      841 tggtgcattg acaaggttct taagcttgat tcaagtaaag aaaaaactat aagtgaaaca
      901 atccataaac aaaaatactt atctttaact gaagaaaata ttgagaaaca gcataagttt
      961 gtaggtgcaa gctctaatta tgggaaagaa ggtctaataa tacatgatgt tttgaatgca
     1021 tttcctcttc ataatgattt aaatactatt gcgatgaaga ttgcggtaat tgatgtaaca
     1081 aattcaacac atctttctca atataagagc aggttgtctc tatatgattt ggctaaagta
     1141 attctggaga ttccaaattt tgatgatcgg ttggcaaaag gagatcctca gttgataaat
     1201 attatcgcaa ggaacattgg tgcagttaat atgttctcat ttgcatccaa gtactgtaca
     1261 tatcataatg tggaagtgtg tgggagagat gattactcca tctttgatgg tatcgttaaa
     1321 aatactcttc cacactatat ccaaggacta actacgaata agattgatac gtggcgaaga
     1381 agttttgatt acgaggcatt taacgaatgt gttggaaaac tacttgatga aaataatata
     1441 catattcctt ttagaagaag aaagttagat cacttcttgt ggtatgcgaa taggtagaat
     1501 ataagaaaaa ggcatcgtaa aagtgattct ggataaaatt aatgggggag gaaataatag
     1561 tgggattatt tgattttcta aagaaaaaac aaggaaaggt tagtaaacac gttgattatc
     1621 aactggcttc tagtctattt cgtactggta aagtttttgc tgaaaatttt aggttatcgc
     1681 tgaaagcccc ggaaatacgg gcatttcgca ggatatgaga actcaattta ctaccaattt
     1741 actactttcg gattgagccg catatatgct gaaccatact gctaataccc gccaccccaa
     1801 atacggcaca tcataaattg aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat
     1861 gggagaacag gacgccgctt ttttatgccc aaatagagaa aggaggcgac gccatgctat
     1921 ttacttccgg ccctgaccgg gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc
     1981 actacgacgg cggcggggcg gacgcgccgg aggattgcgg cacctgccga ttttaccgcc
     2041 cggattggaa atatcaattc tgccagtttg cagagtgtcc ctatcagccg ggcaagctga
     2101 ccgactatgc agcggtcaaa ttcacagtga aaggagcgaa caacgctatg gcagttttcc
     2161 gtgtggaaaa gaacaaaggc tatacggtga tgtcaaatca ccacctgcgg aataagaacc
     2221 tgaccttaaa ggccaagggc ctgctgtcgc agatgttgtc cttgccggag gattgggact
     2281 ataccctaaa aggactgtcc tatatcaacc gggaaagcat cgacgcgatc aggacagcgg
     2341 tatgggagct tgaaaaagct ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca
     2401 aaatggccga tatggtctat accatctacg aacagccggt attggaaaac ccggtattgg
     2461 aaaatccaac atcggataac ccggtgttgg aaaatccgac atcggataat ccggcgtcgg
     2521 aaaatccaac gcaattaaat aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa
     2581 taattacaga tggacaaagt acccattcca ttcctatccc atccccgacc cctgccccta
     2641 tggagggcga ggcagcggct acgccgccgg aacggaaaag gacaggaaag gatgaggctg
     2701 tacggattta tcgggaaatc attttggaga acatcgagta tgcctatctc attcaggaca
     2761 ggagcattga ccgtgaacag cttgacgaaa tcgtagacct gatgctggaa accgtctgca
     2821 ccgcccgaaa gacaatccgt gtcgccgggg acgactaccc tgccgagctg gtgaaatcca
     2881 agtacatgaa gctgaccggc gaacatatcc gctttgtcct tgactgtctg cgggagaaca
     2941 caacgaaagt ccgcaatatc aaacaatacc tgcgggctat gctgttcaac gcccccagca
     3001 ccatttccaa ctactacacg gcccttgtcg cgcacgacat ggcacagccc gattggggaa
     3061 agcctaaatc cggcctgccg gactattcct gcggcgaggg cgaaagcctg taacgagaaa
     3121 aggaggacga ccctatgaag cagggcgcat tgatttttga cgagcgaaca gaccgttacg
     3181 acatccgctt tgatctggcc gactattacg gcggcctgca ttgcggggag tgtttcgacg
     3241 tgatgatcgg cggcaaatgg aggcccaccc gcattgaaat ggccgaaaac tggtatcttg
     3301 tcggcatccg ggccgacgac ctttccggcc tgcgggtgcg gatttgacct atatctacct
     3361 gcggcgtctg gtggttccgc cttagacgcc gcttttgact ttctgacaag gagggatgcg
     3421 attgcaggaa gaagttactc aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac
     3481 ggctcaagtg ctgcaagcgg cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa
     3541 cacgctgcac cacggccagc agagcctaaa gcagcttaaa aagcatgggg ctgatctctc
     3601 caacattgag atcaccgagg ccaacattgg agcgttcaag ccatgcgcca agaaatacgg
     3661 cgtggatttt accttacgaa aagataagac cacccagccg ccccactacg ttgtgatttt
     3721 caaggccaag gatgcagata accttgatct ggcgtttcgg gagtttaccg ccaagacgct
     3781 ttccaaggag cagcggccct ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc
     3841 ggcccagacc aagcagcggg ccaaggagaa aatcaaggaa agggggctgg aactgtgaag
     3901 cctgacgtaa agaaattgct gatcctgaac ctcccgtatc tgatttttgt gtacctgttt
     3961 gcgaaatgcg gggaggcgta ccggctggcc gctggtgctg acctgtcggg gaagctcctg
     4021 cattttgcgg atggttttgc ggcggctttt gccaacccgc tgccaagcct gcacctgttt
     4081 gacctgtgta tcggtatcgc cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc
     4141 aagaacgtga agaaataccg caaaggcatg gagtatggca gcgcccgctg ggggacggcc
     4201 aaagacatag cgccttacat cgaccccaag tttgaaaata atatcctgct cacccaaacg
     4261 gaacggctta ccatgaccgg caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg
     4321 ctggtgatcg gtggcagcgg ttcagggaaa acgagatttt tcgtgaaacc caacctgatg
     4381 caatgctttc cgaccacgga ctatccgact tcgtttgtgg tgacagatcc gaaaggcact
     4441 ttggttctgg aaaccggcag gatgttccag caggcgggct accgcattaa aatcctgaat
     4501 accattaact tttccaagtc catgaagtac aacccctttg tctacatcca ttcggaaaag
     4561 gacgtgctaa agctggtaaa taccctgatt tccaacacca agggcgaggg ggaaaagtca
     4621 gcggaggatt tttgggtgaa gtcggaacgg ttgttttatt cggccctgat cggctatatc
     4681 tggtacgagg ccccagcgga ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc
     4741 agtgaagccc gcgaggacga cccggagttt caaagccccg tcgatctcat gtttgagcgt
     4801 ttggagcaga aagaccccga ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc
     4861 gcaggcaaga cccgttcttc catcctgatt tcatgcggtg ccagacttgc gccctttgac
     4921 atccgggagg tacgcgagct gatggaggac gacgaaatgg agctggacac catcggggac
     4981 gaaaagacgg tgctgtatct cattatgagc gatacggaca cgacctttaa tttcattctg
     5041 gctatgctcc aaagccagct aatcaacctg ctttgtgacc gggctgatga tgtgtacggc
     5101 gggcggctgc ctgtccatgt acgcctgatt ttggatgaat ttgccaacat tggccaaata
     5161 cccaatttcg ataaactcat tgccaccatt cgaagccgtg aaatatcggc ctctattatt
     5221 ttgcagagcc agtcgcagct aaaagcgatt tacaaggacg ccgccgagat catttcggat
     5281 aactgcgata gtgtgctttt ccttagtggg cgcggcaaaa atgccaagga gatttccgat
     5341 gcgctgggga aagaaacgat tgacagtttc aacaccagcg agaaccgggg aagccaaacg
     5401 tcccacggat tgaactatca aaaattagga aaggagttga tgtcccaaga cgaaattgcc
     5461 gtgatggacg gcggcaagtg tatcttgcag ctacggggtg tgcgcccgtt tttatcggag
     5521 aaattcgata ttaccaagca tccccggtac aaataccttg ccgacgccga caagaagaat
     5581 acctttgacg tggacagatt tttgactatc acgcgccgga aacggcagca agtggtaacg
     5641 caggatgaag tattcgacct gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa
     5701 taaacagcgg cttttttcat tccaacaaca aaatcaaaaa atcattatga gccggaaacc
     5761 ggcagaaaga ggtaaattta tggcattttt taattccgca gttactactt tgcagactat
     5821 cgtgatcgga ctgggcggcg ctctgtgcgt atggggcggc atcaatctgt tggagggata
     5881 cgggcaggac aatccggggg caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg
     5941 cgtggccctg atcggtgtca ctctggttcc cctgctgtcc gggctgctgg gctgatcctg
     6001 gcctttgtcc ctgtccaaac ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg
     6061 agggcgcggg aaaggaggtg ctacccgtga ttagtgaaat cattgaagaa tggataaaag
     6121 gcatcctcat ggacggtatc acgggcaacc tgtccgggct gttcgatacg gtaaatgcca
     6181 aagtcggaga aatcgccgcc gacgtgggac gaaccccgca ggcatggaac agcggtattt
     6241 tcaatatgct gcacagcctg tcggaaaccg tgatcgtccc catagccggg gccatactcg
     6301 cccttgtcat gtgctatgag cttatccaga tgataacgga aaagaacaat atgcacgact
     6361 ttgacagctc catgttcttc cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa
     6421 cgaacacatg gaacatcgtc atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa
     6481 gcgccggtgt gattatcggg cagacatcca ttgattttaa caccctgata ccagaccttg
     6541 aaagccaatt ggaggctatg agcgtgggcg ctcttttggg gctgtggttc caaacccttg
     6601 ttgtgggcct caccatgaat atcctttcca tctgcatttt ccttgtgacc tatggccgca
     6661 tggtggaaat ctatgttgtc acgtcgctgg ggcctatccc ccttgccaca atgggcaacg
     6721 cggaatggcg cggcatgggc caagggtatc tgaaatccct gctggcgctg ggcttccaag
     6781 cctttttaat catggttgtg gtgggtatct acgcggtgct gatacagaat atcggttccg
     6841 cttccgacat atccggcgca atctggggct gtatgggcta tacggtgctg ctgtgcttct
     6901 gcctgttcaa gaccggcagt atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc
     6961 caagcataca caggaggaac ttgtttattt caacgtacaa catcatttac gctgacccgc
     7021 cgtggcggta tgactgcaaa aacgtacagg gcgcagcgga aaatcactat tccaccatga
     7081 gcattgacga gctgtgcgcc ctgccggtgg aacggctggc gtccaaagac tgcctgctgt
     7141 ttttgtgggc cacattcccc atgctgccgg aagctctgcg gttaatcaaa gcgtggggct
     7201 tttcattcaa gaccgtggct tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt
     7261 tttacgggct gggccgctgg acaaggggaa acgcggaaat ctgcctgctg gcaacacacg
     7321 gcaagccgaa gcgtcgttct gcctctgtcc atcagtttat tatcagcccg attgaacaac
     7381 acagcaaaaa gccggatgtg acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc
     7441 gcgtggagct gtttgccaga cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg
     7501 aatgtgacct tatcctgacg tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag
     7561 gacttaaccc atgtgaaaag caaggttgtg tttgggctta ccaaacggca actgatctgt
     7621 ttttccgggg cgctggtgat cggcgtcccc ttatattttc tcacccgtga ctatcttacc
     7681 aacagcgcgg cggccctgct catggtattt gccatgctgc cgggcttttt gcttgccatg
     7741 tacgagcgtc acggccagcc ccttgaagtg gtgattatgc agatcatcca gtgctgcttc
     7801 atccagccaa aggagcgtcc ctatcagacc gacaacacct acaccgccct tgtgcggcaa
     7861 taccagatgg aacaggaggt aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga
     7921 aaaaacaaag ctgacccgcg cccagcgaaa agaaattgac gcggtaatcc gtaaatataa
     7981 gggcgacggc aggccccaca cggcgcagca gagcatcccc tacgaggtca tgtacccgga
     8041 cggcgtgtgc cgggtatcgc ccggcgtctt ttccaagtgc attgagtttg cggacatcag
     8101 ctatcagctt gcccagccgg acacgcagac ggctatcttt gaaaaactgt gcgacctgta
     8161 taactatgtg gacgcctcca ttcatatcca gttttctttc ctcaatcgga aagttgaccc
     8221 ggtgcagtac gccaagagct ttgagatcgc gccgcagggg gacgacttcg acgacatccg
     8281 ggccgagtac accggcattc tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa
     8341 gaccaaatac ctgacattca ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt
     8401 gaagcgcatc ggctttgacc tgttaggcta tttcaagagc atgggggccg tagcccatgt
     8461 gatggacggc tgggaacggc tgaacctgct gcatggcgtg taccacccgg acggcgagat
     8521 attcaatttt gactggaagt ggctggcccc gtccggcctc tccaccaagg attttattgc
     8581 cccgtcctcc ctgtgcttcg gcaatgcaaa gacattcggc atgggcggca aatacggcgc
     8641 ggtaagtttc ctgcaaatcc tgtccccgga gctgtcggac gatatgctgg ccgacttcct
     8701 caatacggaa agcggtgttt tagtcaatct ccatgtgcag gccattgaac agaccaaggc
     8761 gatcaagacc atcaagcgca aaattaccga ccttgacgct atgaagattg cggagcagaa
     8821 aaaggccgtc cgcagcggtt acgatatgga cattcttccc agcgaccttg ccacctacgg
     8881 cgaggacgcc aagaagctgc tgacaaagtt gcagacccgc aatgaacggc tgttccagtt
     8941 gacgttcctt gtcctcaacg tggccgacac caagcaaaag ctgaataacg acgtgttcca
     9001 agccgcaggc gtggcccaaa agcataactg tccccttgtc cggctggact accagcagga
     9061 acagggcctt gcaagcagcc tgccgctggg cgtcaatcag atcaagatac agcgcagcct
     9121 tacgacttcc agcgtggccg tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg
     9181 cgcggccatg tattacggca tcaacgccaa gtcccgcaac atgattatgc tggacaggaa
     9241 gcaggcccgt tgccccaatg ccttgaagct cggcaccccc ggcagcggca aatccatgag
     9301 ctgtaaatcc gagatcgtca gtgtgttctt aacgacgcct gacgatattt ttatttcaga
     9361 cccggaggcc gaatattacc cccttgtcaa acggctccac ggacaggtaa tccggctgtc
     9421 gcctaccagc aaggactttg tgaacccgct ggacatcaac ctgaattatt cggaggacga
     9481 taacccgctg gccctgaaat ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg
     9541 caagaacggg ctggaagcca ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta
     9601 ccggccctat ctggcagacc cccgcccgga gaatatgccg atcctgtccg acctccacaa
     9661 agcgttgtta gatcagcatg tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta
     9721 tgtgtccggc tccctgaacg tatttaacca ccgtaccaac gtggacatcg gcaaccggct
     9781 tgtgtccttt gacattaagg agctgggcaa gcagctcaaa aagctgggga tgctcattgt
     9841 ccaagaccag atatgggggc gcgtcacggc caaccgaagc caaggaaagg ccacatggta
     9901 ttttgccgac gagttccatt tgcttttgaa agaggaacag acggcggcct attctgcgga
     9961 gatttggaag cggtttagga aatggggcgg cattccaaca ggtgccaccc agaacgtcaa
    10021 agacctgctt tcctccccgg aaattgaaaa tatccttgaa aacagcgact tcattacgct
    10081 gctcaatcag gcgtccgggg atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga
    10141 acagcagaag tacatcgaca attccgaacc cggcgagggc cttttgatct ttgaaaacgt
    10201 ggtgctgccc tttaccaacc ccatcccgca caacacccag ctttacaaga ttatgacgac
    10261 ccgcttaaac gaggtggcgg gagtatgacc cggcgacgcc acaaaggagg tgaaccacca
    10321 tgaaagagct gaaagccaag gcgaaagtga cgcaggccat gacccgcgac gggctgacgc
    10381 tggaaaatca ggccaccggc gaggtggaga acatttccag ccgggaggcc gaacaggatt
    10441 tgtccgcgcc ggggccgggc ggcaccgccg ataagctgct ggaacgggcc gacgccatcc
    10501 atgacggcca taaggccaag aaagccgcga agcaggccgg ggctgtggcc gccgaggggg
    10561 acgctgccct gcaccggcca tccgcccggc tccaactgtc ggaggaagaa cgggccgacc
    10621 cggatttaca gccctatatc cgaaaatcgg acaaacgggc cgataagctg gataaggccc
    10681 gcgccgccat ccccaagaag cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg
    10741 gcaaagcaaa gaccgtcctg cactttgaca aacaggacaa ggggcctccc tccctgaaac
    10801 ccaatccggc aggccgtcct gtttccgagg tttttctatt cggccacggg aaaatccatg
    10861 aagtcgagca tgaaaacgtg ggtgtggagg gcggccataa gggggaggaa cttgccgagc
    10921 ggcaggccgg aaaagccatc cgcagcacca tccggcacca caggctgaaa ccctactggg
    10981 acgcggaaaa ggccgagcgc aggctattgt ctgccaacgc ggagtatttc taccagaaat
    11041 ctttgcggga taacccggag atcgcacagg cggccagcaa ccccatttcc cggttctggc
    11101 agaaacagca gatcaagcgc cagtacgcca aggccgcccg gacagcgggc cagaccgcag
    11161 gcgcggcggc acagggcgcg gccaagacag ccggggcctc cacgaaagcg gccaaggccg
    11221 cagccaaaga aagtgaacgg gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg
    11281 ccgtccttgt gttggggctt ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg
    11341 ccatgttcgg cagcgccgga agcggcatca cggcgtcctc ttacttttcc gaggatagcg
    11401 atatgttggc cgccgaggac gcctacgcgg agctggaagc cgagctgcaa gactatctgg
    11461 acaactacga ggccacccat gactatgacg aataccattt tgaccttgac gagatcagcc
    11521 atgaccccta tgtgctgatt tccattctgt cggcgctcca tgacgggata tttaccatcg
    11581 gggaggtaca gggtgatttg tccatgctct ttgacaagca atatatcctg acagaaaacg
    11641 tcgtggtaga aacccgttac cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca
    11701 cccatacaga cacctaccaa gtgccgtatg actattacat ctgctacgtc acgctggaaa
    11761 acttcgacct gtcccatgtg ccagtctata tcatggacga ggaaacgcta agtttctatt
    11821 ccctgtatat gtccacgcta ggaaaccggc ctgatctgtt ttccggcagc cagtacccca
    11881 acgcctccac cctgacgccg ccgacctact acgacgtacc cccggaggct ttggaggatg
    11941 ccacctttgc cgccatgctc aaagaggcgg aaaaatatat cggttatccc tatgtgtggg
    12001 gcggcagctc tcccagcacc agctttgatt gcagcggctt catttcgtgg gtaatcaatc
    12061 atagtggatg gaacgtggga cggctggggg cgcagggcct atataacatc tgcacccctg
    12121 tttccagttc gcaggccaag cccggcgacc ttgtattttt caagggaacc tacgacaccc
    12181 ccggcgtgtc ccacgtcggc ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc
    12241 cgatttccta cacgaacctt acttcaaact actggcaatc ccatttttac agttacgggc
    12301 gtttacctta acgccagaaa ggagctttca attatggcaa ccaaacttga ccgcatcgaa
    12361 aaggacatcc agaaaaccaa gtctaagatc gcggagtttc agaaacagct tcgggagctg
    12421 gaaacgcaga aaaccgaaca ggagaatttg cagatcatcc agcttgtgcg cggcatgaac
    12481 atgaagccgg aggaatttgc cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc
    12541 gcaacccctt accatgaaca ggaggacaat gcccatgaag aataagacca gacggatttc
    12601 ccgtattctc cccgcacttc tggccgtgat gctctgcgtc acggcctttc ccgttaccgc
    12661 ccttgcaggc ggcaatgacc cggcaccggc cccgctgccg gaggctacaa ccgaacccgc
    12721 tacgggcggt ttggagcctg aaacagacga aaccggcacc gccacggacg ggacagaaac
    12781 cgaggccgaa aaagataccg gccctctgac cggcaaggac atcaccgacc tgttttccgc
    12841 gctgttcggt tccaaggtga gcattgccgc cacggacgac ggcatccaga tcaccaccgg
    12901 caccgacaaa aaagaacctg aacagaccgg caccgtcacc accaacggcg gcaggctgaa
    12961 tgtccgcacc ggcgcggggc ttgacaagac cgcgttcacc cagcttccca atgggacgac
    13021 ggttgaggta gtcggcacag acggcgactg gattaaaatt ctcttgccgg agcgcatcgg
    13081 ctatgtccat tccgactata tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg
    13141 cggtttttcc ctctccattg acccggagga aattgcttct ctgctggaac tgttcaatgg
    13201 cggcggggcc agcgccgccc tgtccccgga cgggaacctg tcactgattg acgacatcgg
    13261 cagcgccgcg aaaaatggca agcagtttat caccgtggaa accaaaaacg gcaatgtgtt
    13321 ttatctgatt attgaccggg acgacgaggg aaaagaaact gtccatttct taaatcaggt
    13381 ggatgaagcc gatctgctga cgctgatgga ggacggggaa accgtcaccc ccgctgctgt
    13441 ctgctcctgt accacgaaat gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa
    13501 aaccaacctg accgagtgca gcggcccgga gccgcaggaa ccccagccgg aggaacccga
    13561 agccccggag gaagaaccca agggcggcgc gggcggcctg atcgtgttcc tgctggtggc
    13621 gctggcgggc ggcggtgcgg ccctttacta tttcaagttc aaaaagccca aggctgaaac
    13681 caccggcagc gacgatttgg gcgagtatga ttttggggag gacgaggatt tggaggacga
    13741 ggaacccgaa accgagtttg acccggaagc tgattttgtg agtgaacagg aggacgagga
    13801 atgacatata aacttgtaat cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg
    13861 atcggggcca cggagaaaca cgacggctat ttggagggga acggctatct ggtgagctgg
    13921 tgcatcggac atctggtgtc ctttgccgac gccggacgct atgacgagcg tttcaaaaaa
    13981 tggcgctatg aggatttgcc cattctcccg gaaacatggc agtacatcgt ccccgacgac
    14041 aagaaacagc agtttgacgt tctctgttcg ctgatgcagc gccccgacgt gaccggcctt
    14101 gtgtgcgcca ccgacgccgg gcgcgagggg gaattgattt tccgtttcgt ctaccagatg
    14161 gcgggatgcc aaaagccctt tgagcgcctt tggatttcct ctatggagga cagtgctatt
    14221 aaggacggct ttgcccacct gaaaccgggc ggcgactatg atccgctgta tcaatcggcc
    14281 ctgtgccggg ccaaggccga ctggctgatc ggcattaacg ccacccggct tttttccgtc
    14341 ctgtaccaca agaacctgac tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg
    14401 gacagggacg ccaagattac cgatttcaag aaagagaaat atcacatcgt ccatatcacg
    14461 gcgggcggcg cggacgcggt aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc
    14521 aaggcggctt gtgcaaaagc gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg
    14581 gaacagccgc ccaagctcta tgaccttacc accttgcagc gggaggctaa ccggcttttc
    14641 ggctataccg ccaagcagac ccttgactat gcccaggcac tgtatctaaa gaagctgctg
    14701 acctatccga gaacggatag tcaatatctt accgacgata tgctgcccgc cgtggaaagc
    14761 cttgtgtccg gcctgtggga gctggtgccg tttgccaagg gcctgaacct ttccccgcag
    14821 tttgaccgca tcctgaacag caaaaaggtg tccgaccacc acgccattat ccccaccgct
    14881 gaatttgtga agcagggctt tggcactctg gccgagagtg aaaaaaagct gctgtccctg
    14941 atctgctgca agctcttatg tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc
    15001 acatttacct gtgccgggca ggagtttacc gccaagggca agaccgtcct ttcccccggc
    15061 tggaaagaga ttgaacgccg gttccgttct tccttaaaga ccgacgccga cgaggacgcc
    15121 gaggcggtgc gggagctgcc ggagctttgc gagggccaga cctttgcaga cgtggccgcc
    15181 tctgtcaccg agcatttcac cacgccgccc aagccctaca ccgaagatac gcttttgtcc
    15241 gctatggaac gggccggggc cgaggatatg ccggaggatg ccgagcgcaa gggattaggc
    15301 acaccggcca cccgcgccgc cattctggaa aagctggtgc agatgggctt tgtccagaga
    15361 aaaggcaagc agctcattcc caccaaggac ggcatcaatc tggctgtggt gctgccggag
    15421 gcgcttactt ctccccagct tactgccgaa tgggaaaacc ggctgaccga gatcgccaag
    15481 gggcaggccg atcctgacga atttatggac ggcatcgaga cgcaggcgcg ggagctggtg
    15541 cagacctatt cctgtatcag tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct
    15601 atcgggacgt gtccccgctg tggtgaggct gtctatgagg gcaagaaaaa ctactactgc
    15661 ggaaaccggg cctgccagtt cgtcatgtgg aagaatgacc gattttttga ggaacgcaag
    15721 aaagcgttca ccccgaagat cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag
    15781 ggcctctatt ccgtaaagac cggcaaaacc tacgacggga cggtgctttt ggctgatacc
    15841 gggggcaagt acgtcaacta ccgcattgaa cacaggagct aaaggacagt aacacagcaa
    15901 gcatagggag tgtggctaca ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg
    15961 ggatttttgc ttgttgggga agtccccaac cccctatttt ttgaaacaga acagagaaag
    16021 gagtgaccgc gtgaacgaag tattttctat ccaagtccat aaccggcagt ttttggaaca
    16081 gggcaaacaa ggcgtctggc tggatttgcc caccacaacc gaaacgctgc aagccgccct
    16141 gcgggatgtt ggcattactg ccgacaatcc gcaggatttt tttatcaacg ggtattcctg
    16201 cccggaggac agacaccttg ccattcccta tgacatggtg ctggccgccg acgtggacga
    16261 gctgaatttc cttgccgccc ggctgggcca gcttgacgcc gccgagatcg cagagctgaa
    16321 cgccgccctg caaaatcccc gtggcggctt tgagaatatc ggccagatca tcgactacac
    16381 cgaaaatgtg gattactatg tgcatcttcc cgacgttagg ggggatggac agttgggcga
    16441 ctactacctg aaccgttccg gcatggtgga tatgccggag gaatggaaag ccgggattga
    16501 tacggcgcag tttggacgcc acattgccca gcaggaacaa ggcgttttta cccagtacgg
    16561 ctatctggtg agaagcggcg acgaatggca gcgcgtccat gagggccagc cggtgccgga
    16621 ggaataccgg gtgatgcact acccccagcc ggaagctgac cgggaggaac cgcaggccca
    16681 gcagccggag gcatccgaaa gccccgacaa agcgccgccc ccggttatcc cgatcatgct
    16741 ggacggcatg aacagcaaag aacgcatgaa agtgattact gaccgtctgg aaaccggcgt
    16801 acaggagctt tttgaaagta gccgctatat ggcttactta tccgctatgt ccaagatgca
    16861 cgattacagt ctgaacaata cgctgctgat cgccatgcag ggcggttcga ttgtggcggg
    16921 ctataacagg tggcaggatg aatttgaccg ccatgtgaag cggggcgaga aaggcattaa
    16981 gatactggcc ccggccccgt tcaaggttaa gcagcagatg gagaaaatcg acccggccac
    17041 ccagcagccc gtgatcggcg cggacggcaa gcccgtgacc gaagaacagg aagtgacggt
    17101 tccggccttt aaggtggtga cagtctttga catcagccag accgagggca aggattttcc
    17161 catgccctat atcagtgaac ttaccggcga cgtgcagcag tatcaggatt tctttgccgc
    17221 gctggaacgg acatcaccct atcccataag cattgagccg atttcagacg gggccaaggg
    17281 ccgctgcttc tacgcaaaac agcgcattgc catcaacgag ggcatgagcg aattgcagac
    17341 cctaaagacc gccatccatg agattgccca tgccaagctc cacgccattg accgggaggc
    17401 ccccgccgag gaacaggcgg cccggccaga ccgcagcacc cgcgaggtac aggccgaaag
    17461 cgtggcctat accgtctgcc agtattacgg gctggacacg tcggactatt cttttggcta
    17521 tgtggccgat tggagcagcg gcagggagtt atccgagctg aaagcctccc ttgaaaccat
    17581 ccgggccacg gccagcgaat tgataaccga gatcgacggc cattttgccc agcttcagca
    17641 ggagcgcgag gcccggcagg aacaggaggc cgtcacggtt gacgttaccc cggaggcagc
    17701 ggccccggcg caggccgagg aagaacaggc cccggccctt gatccggcag ccgagccgct
    17761 tgtgactgtg ctatggagcg aaagccccca gctaaaggac ggccagcaaa tgccgttgca
    17821 tgaggccgac gcgctgtttg cccgtctgga tgcagcatat ccggcagagg caggctatga
    17881 caaaaccgcg ttccgcattg actgtatctt tcagggcgag ccggacagct acgaggggcg
    17941 gcaggatttt ggagataggg acggttccct gatcgaacat atccagagct accaccaatt
    18001 ttacgcgcag gacgaaaact ggaaaaagca tgtaatcgct acaaccggca tagagggctg
    18061 ggagcaggac aaggcccatc gggatatgct gttaggcgag tttgtcccct atatgaagct
    18121 gcattgcagc cttgcccaaa tggaacagac ggcgcagggc cgcttacagt ccggggaaac
    18181 cctgacgccg gaggaaacgg cctatttggg ggcgcttctc tcttatgtgc aggagtgccg
    18241 ccccatgctc aatcagggcc agtatcagtt gccggagccg ccgaaactgg ctgacttcga
    18301 ccagagcttg caggactaca aagcacaggt acaggccgag atcgcgcagg aggccgccgc
    18361 agccggtatg accgtggagg aatacgcggc ggctggctat gaagcacccc aacaggacac
    18421 cttttccatc taccagcttc gaggcgggga ggaaacccgc gactatcggt ttgagccata
    18481 cgagcgttta caggccgcag ggctggccgt ggacagtgcc aactatgacc ttgtttatac
    18541 ggcaccgctg gataaaagca cgtctttgga ggatatttac cgcacattca acatcgacca
    18601 cccggcagat tttaggggac acagcctttc cgtttccgac gtggtggtgc tgcaccagaa
    18661 tggacaggac accgcccact atgtagacag cttcggctat atggaagtgc cggagttttt
    18721 gcgggaaaat cccctgcgta ccgccgaact gtctacggag cagaacgaaa acatgattga
    18781 cggcattatc aacaatacgc cgcccgaacc gcagccggag gaacccgaac agctatcttt
    18841 tgaggatgtg gcagttgctt ccggccagac acaggaggaa cccccggagg ctgcgccccc
    18901 gcaaacggct gtccgctatt atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc
    18961 catgagcttt tctgactacc ggcccggcag cgccacggcg gaataccggc actatgtcga
    19021 tcaggccgtg gaaattgcgg agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa
    19081 gatagacagc ctccttgaca cctacgcccg caagctggcc gagaacatga ataagggcta
    19141 tgagattacc gcccgcgtcc cctctgtcct gattgccggg ccgtccaatt tcccggtacg
    19201 ggcgaaagaa aagcagaacg ccgccagtga ccgcaacatg gaggaatttc agtacattca
    19261 gggcttgctt gacaaaatcc gcagcaccgg catgggtggt atcagcgccg acgatccgca
    19321 ggccgtttcc aagctggaaa agaaactgga aaagctggaa gcgtcgcagg agttgatgaa
    19381 ggccgtcaat gcctactacc gcaagcatgg gacgctggac ggatgccccc atctgaccga
    19441 aagaggcatt gaaaacctga aagcggatat ggcgtcgggc tggcactatg agaaaaaacc
    19501 gttccagtcc tggcagctct ccaacaataa cgccgagata cgccggttaa agggccgcat
    19561 tgaggaattg acccgccaaa aggaggcggc ctatgtggga tgggagtttg acggcggcac
    19621 tgtggagatc aaccgggtgg ccaaccgcct gcaaatcttc tttgagggga agccggatgc
    19681 tgctgtacgc gacgagctga aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg
    19741 gcagcggcag ttaaacgaca ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc
    19801 cctttccggc gaaaagccca gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc
    19861 ctctatccgg gcgcagctca acgcggccaa gaccgaacag gccaagcagc caccggcgcg
    19921 ggaaaaatca aagggattgg aggtagagta aatgcagaag tttgaaaatg tggacgttct
    19981 ggccgcgctg gaacagatca tgcggcagaa caccgcgttt tatcaaagtg actttggcat
    20041 tgacaaagac attatcaggc gggatgccgc cagcgatcag gccgcagaca agaccctgct
    20101 ctggatgtcc cggccctccg ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga
    20161 cacccgacag tacaacacat ggaaatttta cggggagcag acccgtgaca agatactggc
    20221 ctacgcggtg gagctgaccg gcacacaggg cggcactatc cggggcaatc tctatgagct
    20281 ggactatccg cagcatttcc ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg
    20341 gctcttttat gagcatggca cccgtgacat cccggagagc cagcgttttg acggaagccc
    20401 tgaccgggta ttgggaaact ttttgtacta tgaggcccag ccccatgacc cggctgtgct
    20461 gcaggaggcg ctacggacgg agcagcacag ccgggggcgg gccgtccccg gcgatttcaa
    20521 agtccacgtc accgctttgc atgacagccg gattgaaacc gaggcccggc gtattgtgga
    20581 cagcctgaaa gatctgtccg cgcctaacag ccccaataaa acgcatttca tggtggagat
    20641 ctccccgcac tttgcggcga ttgcttccac caaggataat gaccggctct ttgccatgct
    20701 gccctataaa tccctgtgtt ttaccgggat gaaaggcagg catggcgtgt atgccgtgat
    20761 taaccgggac gagcgccggg atgtgagtat tcgcaggccc cggccctcta tccgcaagca
    20821 gctatccgac agcaaacagg ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa
    20881 tgaattggag gtataaggaa tgatgcactt taccgtggag gaagaaaacc tgatctgtat
    20941 gtaccacaat gcagaccggc gcagaacgat aaacaggctc accgccgtca tgccggttat
    21001 ggatggggat atgcgggcgc aggcccagca gaccctttcc aagctggaac gcatgacgga
    21061 tgccgactat gacggccaga agttccactt cacagacgaa taacgaacag cagacgccgg
    21121 gcgcggcggc catgtggtcg cttgcgtccg gcgtcttttc tttttgccta ttcctacgca
    21181 tttagaaccc ggtattgtcg gggataggat gaagtattcg cttgcccctg caaacatgcg
    21241 ggagaagcct tgcagcgggc ggttttcaat cccttaaatg cgtaggttgc agctataatt
    21301 agacccctga aacagatata gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg
    21361 atagccgcaa agcggcgtaa gagggtgcag ccctcttgcc gtctatctct gttccagcgg
    21421 gaagctcggc aatgcttcca gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat
    21481 gactttcttc tttttgccgt ttgcctgtct gatctccacc gtggaaagct cgttgatgta
    21541 gtcagcataa tgggccaaca ctttttccgt ggcccacctc tcaccggcaa cggcggcttt
    21601 gatcgtttca tagtccagca gcttttcttg tccgtccatc ctgatacccc cgtcagactg
    21661 attttttcta tgataccata ttcggaagca tggaaccaga gaaaacggcg cttttctgct
    21721 ggtaaattct acgcatttag aacagcactt cggcggggat aggataaaac acccacatac
    21781 cccgctgacc atgccggaaa agccttgaaa tataagggct tttacagctc taagtgcgta
    21841 ggtttccagt atcttttccg ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag
    21901 caggccgcgc agtatttggc ccggttagac ttcggcgtga acgtggcccc gcactcacag
    21961 cagcgtttca cttcgtcccg gtctttggtg atctcggcat acagggccgc atccagcggg
    22021 aggacagcgg ccttaaacca gcggcacaga agcgaatagg aaatgctctg tacgcaaata
    22081 cattcttcgc cgtcgtccag caacaggcag ttgccgtcac aatagttgca gcactcatgc
    22141 gtcagcttcc gggccttgcg atattgaggg taattcattc gtggaatact catagctctt
    22201 gcgtccattc ctttcccggc gtcatacgca aaatgccgtc cacattctgc ttgatgatgc
    22261 cgtattcctg catctgcttt ttcgcttcgc cgtacttctc atacagccgt tccttttcct
    22321 cggccaatcg cttgtactcg gctttcaggg cgtacagatc ggggagcttt ttcacaccct
    22381 gtccctttag ggcctttgcc gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc
    22441 ggaattttgc cttatccgct gcattgcggt attccatagc cacgggccag agctgcttgt
    22501 aggtagaaat attctttatc agcaccgcca tatccgcaag ccggtgttcc atagctttga
    22561 gggtagcggc ggcctcgtcg ctggcggcgg tgatctcggc cactttgctt tccaaatccg
    22621 ggtaggtgtc tatcccatgt tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat
    22681 tgtggagctt cgcccacctc tcatagcctg ccgactgctg ggccttaatg ttgttttcca
    22741 gatcaatccg caaagaaatc cccttgcgct ctttgggcgc tctggtgata acgcggcctt
    22801 tgatacgctc tttgatggct tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa
    22861 agcgttcctg tccgggagcg cggaaagaaa taaactttcc cggcttgatc tcatagccct
    22921 gcgcctgcat gagccgcagg aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa
    22981 tggctgcttt gagcttcgct ttccagctcg tccctttccg ctgggcgtcc cactcggcat
    23041 agctcttgcc cttactctgg ccgggcttca ccacggacaa tccgttttcc ttgcacagcc
    23101 tgtcgctggt gcgccggata taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat
    23161 ttaccatatc cacggcacag aaaataatgt gattgtggac gtggccctta tcaatatggg
    23221 tagtcagcac atagggatat ttcccttgca gcacttcctc ggcaagctgc ttcccgatct
    23281 catgggcctg ttccggcgtg gcttcgccgg gggcaaagga ttgtatcagg tggtggccca
    23341 gattattgcc cttttggtat gcctgcgcca gcagcatttc aaattcaatg tccgctgttt
    23401 cataagagca ggcaaaggag gccaccagca gcttgccgtc agttttgtcc gggttctcga
    23461 tgtagtcaag ggcttttttc agcgttgact tgataggatg tatctttgta actgccatat
    23521 ctgcgccagc gcccccttta tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt
    23581 cagcctcttt gcaatctgat tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc
    23641 ccggatgtcg ctggtgtcca tgttgacgat atagccgtca atgagcatct tccgggcgta
    23701 ggctgaaaag ttcttcgtat gcagcagcgc cattttctgt tcaatcatag cccgttcctc
    23761 cggcgtcaca gggacgcgca gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc
    23821 tttccattca ggggtttggg gatttcccca acaagcaaaa cggacgcggc ccggcaggga
    23881 cggggcaggc cgtttttccg ggagtggcta cactccctat gcttgctacg ttactgtttc
    23941 tccactaaca agacgtttcc aaagccccaa actacccgca aaatagccct ctggaaagaa
    24001 aaaagtgcaa aaaaatagaa gctacgctgt gcagctcccg ctaaaagtaa aacagaggat
    24061 gccagttaca catcctccgt ttcattcttc ttcggctttt caattagtat ggcgctgtca
    24121 atcgcgccct cgatgatcgg caggtattct tcggggcaga gtttcagctt gtggctgacc
    24181 ctttgccgca gttcgctttc ctcccgcatg atctccgggt taaagtagcg ttccacagga
    24241 agcccgcagg ctttaataag ctgtatgaca accggcagac tgggaatggc cccgtcgttt
    24301 tcgatgttgg caagatagcg ccagtcaata tttattttct ctgccattgc tttgcgcgaa
    24361 acgcgctttg cttgccgggc cgctttcaca tccgcgccga aagtttcaaa gccgggacaa
    24421 tcttcaatat tcgccatatc gcacctccca cttacattgt atatttcata ctctgcccgt
    24481 ggaatgttgg tatatgcacc tttcttaaag tttatatttc acattttgtt tcttttattc
    24541 ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac tatggactac atgacgttga
    24601 aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat aaactactat tgtgctgcgg
    24661 gacgcatccc cggcgctgta aagatggcga cgatctggct cattcccaaa actgcggaaa
    24721 agccgattga caggcgtaca aagaaaggaa aggcggttac tgaatagtat aatgaaaccg
    24781 gcgcattttg gatatggttt tgaagagaga gaaagcatgg tattttgtca cggaaaaact
    24841 ataagatgat agatggcagg ctgctccaga ccgataaaaa gtattctcat ctgaaacaga
    24901 aagaaaagat ttacacatgg atgtatgaag aagcgaaact ttatcatgac agaaatggaa
    24961 aatgcccgaa acaaagacga ggaagctgct gctgtatcag ccgtatatgg tagaattgaa
    25021 aaggccggca tctggattct ttatggtgaa gtctccagac attggataac tgctggtcta
    25081 atgcgccttt gagtatgggg tgattgattt taataccggg gaggtacggc tttttgagga
    25141 aaatattctg ggatttacat ttggcgatta ttacatttcc tgtaactgca aggacacgga
    25201 tgatgaagat atgaggaata tagacttgct gattttctac tgtcttgaac ggtattagga
    25261 ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg gagtacacac aggccctgcc
    25321 tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag
    25381 gcttggggtg tccccaacaa gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag
    25441 aatatccgta aaggatattc tgatccattg cttgccagta ttatcgggat gttttgacaa
    25501 ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta attattatta tcttgattta
    25561 attcgtttaa accaataata tagatacata caccatgaaa agaagcgagt atcccaatga
    25621 cggatgatta aaatccgaaa agaaatcgct ttgaaactac agggaaacta cagactgtta
    25681 tgttatcttc ttaaaatgga gggattttta tgtcgatacg aattctactt gtcgaggatg
    25741 atgatcatat ctgtaataca gtaagggcgt ttctggctga ggcaggatat caggtggatg
    25801 cctgcacaga tggaaatgag gcatacacca agttttacga aaacacttat caactggtta
    25861 ttcttgatat tatgctgccc ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga
    25921 aaaatgatac tcccattctg atgatgacag ccctgtcgga tgacgaaaac caaatccggg
    25981 cgtttgatgc agaggcagac gactatgtaa caaagccatt caagatgcag attttactaa
    26041 agcgggtgga agccctgctg cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca
    26101 ggctgacact tctgccggag gatttcacgg tactttgtga cggtacagag ctgcccctga
    26161 cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa caaaggcaga accttaacgc
    26221 atgaaatcat tttgtcacgt atatggggat atgactttga gggtgatggc agcacagtcc
    26281 acactcatat caaaaatttg cgggccaagc tgccggaaaa tatcatcaaa accattcgcg
    26341 gtgtaggata ccgattggag gaatcataat aatggaaaga aaagggattt tcattaaggt
    26401 tttttcctat acgatcattg tcctgctact gcttgtcggt gtaacagcaa cactgtttgc
    26461 acagcaattt gtgtcttatt tcagagtgat ggaattacag caaacagtaa aatcctatca
    26521 gccattggtg gaactgattc agaatagcga taggcttgat attcaagagg tggcagggct
    26581 gtttcactac aataaccaat cctttgagtt ttatattgaa gataaagagg gaagcgtact
    26641 ctatgccaca ccaaatgcca atacatcaaa tagttttaga tccgactttc tttatgtggt
    26701 acatagagat gataatattt cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta
    26761 tcaagggctg acaattcggg gaattgttat gattgcgata atggttgtat tcagcctttt
    26821 atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc aaagccttag cggacagtgc
    26881 gaataaaatg gcaaacctga aagatgtacc gccgccgctg gagcgaaagg atgagcttgg
    26941 cgcactggct catgatatgc attccatgta tgtcaggctg aaagaaacca tcgcaaggct
    27001 ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca cagcgatatt tctttgcggc
    27061 agcttctcat gagctaaaaa cgcccatcgc ggctacaagc gttctgttgg agggaatgct
    27121 tgaaaatatc ggtgactaca aagatcattc taagtatctg cgcgaatgca tcaaaatgat
    27181 ggataggcag ggcaaaatca tttccgaaat actggagctt gtcagcctga atgatgggag
    27241 aatcgtaccc atagctgaac cgttggacat agggcgcacg gttgccgagt tgctgcccga
    27301 ttttcaaacc ttggcagagg caaacaacca gcggttcgtc acagatattc cagccgggca
    27361 aattgtcctg tccgatccga ggctgctcca aaaggcacta tccaatgtca tattgaatgc
    27421 agttcagaac acgccgcagg gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa
    27481 atgccgcctt tttgttttga acatgggcgt tcacattgat gatactgcgc ttccaaggct
    27541 gttcacccca ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc gaagcggttt
    27601 aggacttgcc atcgtacaaa aaacgctgga tgccatgagc ctccagtatg cgctggaaaa
    27661 cacctcggat ggcgttttgt tctggctgga tttaccgctc acatcaacat tgtaaatatt
    27721 taaaacttaa atgattttga ccgacaggta taaccctgcc ggtctttttt tgctgctaca
    27781 ggaaaactac agactggcta cagggaaagt acagatgcgc ttgccataat aacaatcgta
    27841 ccagccacaa atcgtagttt tattgcaaag gaggcattcg atcaaatgga aaaaaaaagc
    27901 aactatcatt ccaatgcgga tcatcacaaa cggcatatga aacaatccgt ggaaaagcgg
    27961 gcttttctat gtgcgttcat tatttcgttc acagtctgca cgctgttttt ggggtggaga
    28021 ctggcttccg tattggaggc aacacagata ccgcccatcc ctgcaactca tacaggcagc
    28081 agcactgacg tagtggagaa tttggaggaa aacgctcttg ccaccgccaa agaacaggga
    28141 gatgaacagg aatggagcct gattttagtg aacaggcaga accccatccc cgcacagtac
    28201 gatgtggagc ttgagcaact atcaaatggt gagcggatag atattcggat ttctccctat
    28261 cttcaagatt tgtttgatgc cgcaagaact gatggagttt acccgattgt cgcatccgga
    28321 taccgaacaa cagaaaaaca gcaagaaatt atggatgaaa aaattgccga atataaggcg
    28381 aaaggctaca cctctgcaca ggctaaagcg gaagcagaaa cttgggtggc cgtgccggga
    28441 acgagcgagc atcagcttgg tcttgctgtg gatatcaatg cggacggaat tcattcaaca
    28501 ggcaacgagg tttatagatg gctggatgaa aacagctatc gttttggttt tattcgtcgc
    28561 tacccaccag acaagacaga gataaccggt gtgagcaacg agccgtggca ttaccgatat
    28621 gttggcatcg aagctgccac agagatgtac aaccaagggg tttgccttga ggaatattta
    28681 aaaccagaaa aatgagaaaa ggatgttatg ttatggacag aaaaagattg acacagcgtt
    28741 tcccgttcct gcttccaatg agacgggcgc agagaaaaat gtgcttttat gcgggaatga
    28801 gatttgacgg ctgtcgctat gcgcagacga taggggaaaa atcgctttcc catttgctct
    28861 ttgaaacgga ttgcgcatta tacaaccaca ataccggatt tgacatgata taccaggaaa
    28921 acaaggtgtt caacttaaag ctggcagcaa agaccttaaa cggcttattg ataagaccgg
    28981 gggaaacctt ttctttctgg tggctggtac gccatgcgga caaagatacc ccctataaag
    29041 acggccttac ggtgaccaat ggtaaactca ccaccatgtc gggcggcggt atgtgccaga
    29101 tgagcaattt actattttgg atgttcctgc atacgccatt gacaattatc cagcgcagag
    29161 gtcacgaagt aaaggagttt ccagagccaa acagtgacga gattaaaggg gtggatgcaa
    29221 ccatttcaga gggctggatt gatttaaaag tgcgaaacga taccgactgc acctaccaaa
    29281 tatgggtgac cttagatgat gagaaaatca tcggtcaggt gtccgccgac aaagagcccc
    29341 aagcattata caaaattaca aatggcagta tccagtatgt ccgtgaaagt ggcgggattt
    29401 atgaatatgc ccaggttaaa cggatgcaag ttgccttagg taccggggaa ataatagatt
    29461 gcaagctgct ttatacaaac aaatgcaaaa tctgttatcc cctcccggaa agtgtggata
    29521 ttcaggagga gaaccaatga gaaaaagtgt gggcattact gtttttggat gcgaacagga
    29581 tgaggcaaat gctttccgcg ctttatcgcc ggattttcat attatcccta cgctgattag
    29641 cgatgcgata tcggcagaca acgcaaaatt ggccgctggc aatcaatgcg ttagcgtagg
    29701 ccataagtcc gaggtttccg aggcgacaat tcttgcgctg agaaaggtcg gggtaaaata
    29761 catttctacc cgcagcatcg gctgcgatca cattgatacg actgccgccg agagaatggg
    29821 aatctcggtt ggcacggttg cgtattcgcc ggacagcgtt gcggattatg ctttgatgct
    29881 gatgctgatg gccatacggg gtgcaaaacc caccatgcac gccgtggcgc aacaagattt
    29941 cagattggat cgtatccggg ggaaagaact gggggatatg actgtgggag ttattggaac
    30001 cggccatatc gggcaagcgg tcgtcaaaag gctgcgggga tttggatgcc atgtgctggc
    30061 ctatgataac agccgaaaaa tggatgcaga ttatgtccag cttgatgagc ttctaaaaaa
    30121 cagcgatatt gttacgctcc atgtgccgct ttgtgcggat acccgccatc tgatcggtca
    30181 gaagcaaatt ggagagatga agcaaggcgc atttttaatc aacaccgggc gcggagcgct
    30241 tgtcgatacc ggggcgctgg tggaggcgtt ggaaagcgga aagctgggtg gtgcggcact
    30301 ggatgtgttg gagggcgagg atcagtttgt ttataccgat tgctcgcaga aagtgcttga
    30361 ccatcccttt ttgtcgcagc ttttaaggat gccgaatgtg atcatcacac cccatacggc
    30421 gtactacact gagcgtgtgc tgcaggatac tacagaaaaa acaatcagga attgtctcaa
    30481 ttttgaaagg agtttacagc atgaatagaa taaaagtcgc aatcatcttc ggcggttgct
    30541 cggaggaaca tgatgtgtcg gtaaaatccg caatagaaat tgctgcgaac attgatacgg
    30601 aaaaattcga tccgcactac atcggaatta caaaaaacgg tgtatggaag ctatgcaaga
    30661 agccatgtac ggaatgggaa gccgacagtc tccccgccat actctccccg gataggaaaa
    30721 cgcatgggct gcttgtcatg aaagaaagcg aatacgaaac acggcgtatt gatgtggctt
    30781 tcccggtttt gcatggcaaa tgcggggagg atggtgcgat acaggggctg tttgtattgt
    30841 ctggtatccc ctatgtgggc tgtgatattc aaagctccgc agcttgcatg gacaaatcac
    30901 tggcctacat tcttacaaaa aatgcgggca tcgccgttcc cgaatttcaa atgattgata
    30961 aaggtgacaa gccggaggcg ggtgcgctta cctaccctgt ctttgtgaag ccggcacggt
    31021 caggttcgtc ctttggcgta accaaagtaa acggtacgga agaacttaac gctgcgatag
    31081 aagcggcagg acaatatgat ggaaaaatct taattgagca agcgatttcg ggctgtgagg
    31141 tcgggtgtgc ggtcatgggg aacgaggatg atttgattgt cggcgaagtg gatcaaatcc
    31201 ggctgagcca cggtatcttc cgcatccatc aggaaaacga gccggaaaaa ggctcagaaa
    31261 atgcgatgat tacagttccc gcagacattc cggtcgagga acgaaatcgg gtgcaggaaa
    31321 cggcaaagaa agtatatcgg gtgcttggat gcagagggct tgcccgtgtt gatctttttt
    31381 tgcaggagga tggcggcatc gttctaaatg aggtcaatac catgcccggt tttacatcgt
    31441 acagccgcta cccacgtatg gtggccgccg caggaatcac gcttcctgca ctgattgaca
    31501 gcctgattac attggcgtta aagaggtgat aagtatggaa aatggttttt tgtttttaga
    31561 tgaaatgttg catggcgtcc gttgggatgc caagtacgcc acatgggata acttcacagg
    31621 aaaaccggtg gatgggtatg aggtgaatcg catcatcggc acaaaggccg tggcgtttgc
    31681 tctgcgcgag gcacaaatcc atgcggctgc gcttggctat ggcttgcttt tatgggatgg
    31741 atatcggcca agaactgcgg tggactgctt cctgcgttgg gcagcgcaac cggaggacaa
    31801 gctcacaaaa gaaaaatttt accccaatat agagcgagcc gagttgatta caaagggtta
    31861 tgtggcctca caatccagcc atagccgtgg aagcgcaatt gatcttacgc tctaccacct
    31921 ggatacaggg gaacttgttt caatgggaag taacttcgat tttatggacg aacggtcgca
    31981 tcatacagca aaagggatag gggatgcaga ggcacaaaat cgaagatgct tgcgtaaaat
    32041 catggaaagc agcggatttc agtcttatcg ctttgaatgg tggcactata agttgattga
    32101 tgagccatac cccgatacct attttaattt tgccgtttca taatgaaagt attggttttg
    32161 tatcagattt tggatttgat aaaatctatg ctggtcatgg acaatctttt actggaaagt
    32221 ggttaaagca tcttttgtaa tacattgcaa attatagtat tcaatctgcc cagcggcaga
    32281 aaagaaaaaa accgctgttg ggcagacctt tcccacgccc attttgtatt ttgctggcgg
    32341 cggttttgag aaatcaaggc cgccgttctt ttctggcagc aaaaggtgtg gcggcccata
    32401 ggaggatgcc gctatgctgt tttgctattg ccatgctcta atccacttcc accactcaaa
    32461 aaaagccttg cccctccaca gggctattcc ggttatgagt atgtactata ccatgtaaat
    32521 cagcagcata gcaaagcatt ttctcgcccg gtaggtttgc gacctgccgg gcgatttttg
    32581 tcgtcttttg cggcccggct gtccccaaaa aaattttttg agaaattttc aaaaaggagg
    32641 tatttagcgg gtagctggcg gccttgtcaa agtcttgtta gcggaaaggg agagaaccac
    32701 ctaagtcccc cacagaaagg gggtgagaag atggatgcta tcccccgcga ctatgaagcg
    32761 cggtgcatgt tcgatgcttt ttgcaagacg gtactgcgta acgaggccaa aagctacttg
    32821 gctgaaatga agcgccgccg tgaccgtgaa gtgtcactaa gctctttgtc acaggcagat
    32881 ttggacaagc tctgtacagt agatcactac cccagcgaca gctttacgtt ttcgtcgcat
    32941 ggctatgacc tacatatcaa caatgagctt gttgccgaag cctttgccgc gctgcccagc
    33001 atggagcaga gtattttaat cctgcactgt gttctggata tggcagatgg tgagatcggc
    33061 ggccttgtgg gaatgtcccg gagtgccgtt cagcggcaca ggacaaatac cttatcggag
    33121 ctgcggaaac agctaaaggc gttgatgccg aagggaggtt agacgcggat gaagaacccg
    33181 aactacaagg gcattgagct tttgcccctt tcggtgatcg aggccgcccg cgctggggat
    33241 gccggagccg tagagcgcgt tttgcagtat tacagcggtt acatgaacaa gctgtgtacc
    33301 cggacgctct atgacgaaaa cggctatccg catgtgtgtc tggacgaggc catgaagcgc
    33361 cgtctggaaa tcaagctcat ccacgctatc gtcacgaact gacgaaccgg ccccggcagc
    33421 atgaaacggc ttaccctttc cccgtttcgg tgttttcccg gtggcctgat cgcaccttga
    33481 taaatatgcc cgcaagataa cggcggcgca tcatagggca tacggacaca ttctgtctgt
    33541 gccgagccgg acggctggtg cgccatgata cttttcccta cgggaaaagc ggagcgagga
    33601 acaccacgcc gcaatgtgag cgtggcggct tgcgggcgac gacgtacagg caggactatg
    33661 atactccctt atagccacag tccgagcgtt aaaagcgtcg caggcaatgg gtagggccgc
    33721 gtgagaacca cgcgggggtg aaagtcccgt ggagctgtgc cagcagccgt ccgtctatgc
    33781 ccctaaatca cacaaacctt tgaatttgtg aaagggggct gcattatgaa ccatgatgtg
    33841 cctatttggg aaaaatacac gctgacgatt gaggaagcgt ctaaatattt ccgtatcgga
    33901 gaaaacaaat tgcgtaagct ggccgaggaa aatccggcct ctggctgggt cattatgaat
    33961 ggcaaccgca tccagatcaa acgcaagcag tttgagaaaa taattgacac tttggacaca
    34021 atctaatagt gaaaaagggc cgcagatatg ttataataat ttataagcat atatgcggct
    34081 ctttccgtca tggaaaggag catgacgaga tgtcagaaaa aagacgagac agcaaaaatc
    34141 gcgttttgcg gtctggagag agccagagaa aagacggacg atatgcttac aaatatgttg
    34201 atacctttgg aaaaccgcag tttgtttact catggaagtt agtgcctacg gataagaccc
    34261 cggcaggtaa acgggaggat atttcactcc gggaaaagga gaaagaaatc cagaaagacc
    34321 ttgacgacgg gatagacacc atcggcaaga aaatgaccgt ctgccagctc tacgccaagc
    34381 agatcaggca ccggggcaat gtgaagcaca acaccaaact gggccgggaa cggctgatgc
    34441 gtattctgga acaagatcgg ctggggagct gcccgattga cagtgtgaag ctgtccgacg
    34501 caaaggaatg ggcgcttcgg atgaaagaaa agggcctttc ttataagacc atcaataacg
    34561 acaagcgttc cctgaaagcg gctttttaca ccgccataca ggacgattgc atccgaaaaa
    34621 acccgtttga cttccagctt agtgacgttc tggacgacga tacggaacca aaggtgcctc
    34681 ttacaccagc acaggaagaa agttttctgt cctttattca aggtgataag gtttaccaaa
    34741 agcattatga cgcaatcgtt attctgctgg ggacagggct tcgcatttcg gaactgtgcg
    34801 gactgacgga caaagatttg gattttgaaa accgggtaat catcgtgagc caccagcttt
    34861 tgagaaatac cggcgtcggt tactatattg acgaacccaa gacccaaagc ggcgtcagaa
    34921 aaatccccat gaatgaggaa gtgtatcagg cgttccagcg agtgataaaa aaccgcaaag
    34981 gtgcaaagcc gttcattatt gacgggtatg ccaatttcct gtttctcaaa cagaacggct
    35041 atcccatgac ggcggtagat tatggcggga tgtttggcag gcttgtgaag aagtacaaca
    35101 agagccatga agaagcgttg ccgaaaacga cgacgcccca tgctatgcgt catacattct
    35161 gcacccggtt agccaatgcg ggaatgaacc ccaaagcctt gcagtacatt atgggacatt
    35221 ccaatatcac catgacgctg aactattatg cacacgccac attcgattcc gcaagggcag
    35281 aaatggaacg gctggcagcc tgacacaagg ccggttttac tactccattt actacttttg
    35341 agggggaaat ggtgagaggt aataagaaca tttgtaagta tctaacaata tcaaaaatgc
    35401 cggaaaagcc cggaaacacg ggctataccg acattcaaga acttctaaaa agataatcta
    35461 aaatcccata taattttctg cgcaaaaatt ggaagtggac atatcaaatg tagatgagtt
    35521 tcgtaaaact tttatcgctt ttgatgtgga aacaactggc ctaaattctt cttcagatag
    35581 aattgtagag attggtgcgg tgctatttaa agaaggagtt ccaattcaat cattcggaac
    35641 tcttgtcaat ccaaaagtta gaattgcagc ctcagcttcg gcaataaata atataactaa
    35701 tgaaatgcta aactctgctc caaatgagca ggaagtttac ccccagttgg tagaattttt
    35761 aggagatgca ctagatggaa aaatcactat gtgtgcacac aatgcaagtt tcgattttgg
    35821 atttttaagt aacacatttt ctagacttgg gtatgatgct aaaattaagt atgttgatac
    35881 tctgagtctc tctagaaaac atataaaagg cattgtaaat cataagcaag agactgtagc
    35941 ggaatatttc ggattaataa atgacactgc ccatagagcg gaatcagatg cagatatatg
    36001 cggaaagatt ttttggggtg ttttagatat tattgaggag tcgttggaag aacaaaaaaa
    36061 gcaaatagaa aagatgactc cagacataaa agaacttgaa gcttgtgctt ttattcaaga
    36121 tattattgag aaaaaaggat gcgacaccag ttggatcagg tatcgaaaaa acagtaataa
    36181 ttatgtcgat gtaacttgtc tatatacatt cttaaagttt aagtttgcta agaagggaaa
    36241 ttacatcatt attagaaaag atgctacaaa tggaattgat ttacctatag aaccttgtac
    36301 tgtaagtgaa ggcggtacaa caaatgtgcg agtctatttt cgtagcccct ttgacttaac
    36361 gcctctttca aattatattt tcagtgctta tagcgattgt tataattcta tgaatgaata
    36421 tatgagtgtt agtaattaca ctaaaaaaga agcagagcaa agccttagta tgctgaaatc
    36481 tatttcaaaa tcagcgatga aagagttatt attcgatgca aaaaatcgtg gatatgatga
    36541 gactgaagtt aacctaaata ttgaaaaagt gatatctaga gaagatgtaa ttattaacgg
    36601 gttgcataat cgagtttcat taaacaagat acgaaatata gggaattggg agaaaggatt
    36661 tgatgcaggt tatccatatt gggaacgtgg agaaactgct aggaaaaatg gcaaagtaga
    36721 tgaagctatt gcactttttg ataaagctcg atataacggc tatgatgctc ctgcgctata
    36781 tgattcttat gctaaagctt atcgaaaaat taaggattac gataatgaaa ttctcattct
    36841 tgatgaagga attatgcgta aaacacggca cgatgttggt actttagagg ctcgtaggga
    36901 taaggcaatt aagctattat ttgctaaaca agaggcagag cggatagcta aggaaaaatc
    36961 tgattttcta aaagcgaaca aaaaagagga tgcgattagt gagcctagca agaagcgtgg
    37021 aagacagatt attcaaatga ctgatgatgg aacaataata aaagaattcg atactatagc
    37081 ttctgctgta aaagaagttg gcgttaactc aaaaagtatt agggatgctg caaatggagt
    37141 tcaaaaacat gctggaggat actactggaa atacaaagat tagcagataa tctaagataa
    37201 cctaggatct tcttaataga tacccgtggt gctagttaat tttaatatcc taaagaatat
    37261 ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata gaacactgta agccagtaat
    37321 atgctttcaa atctactctc tagcccttta acagaacgtg agttgaaatt ttgacaacct
    37381 aatttttcta aggaagaaaa aacagtccca actgtttttc tttgttttcc aagtaagctg
    37441 tggtctagtt tttctgattt atccatgttt ttacgtggcg gaatggatat agtaatgccc
//
LOCUS       DACCQI010000008        37246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCQI010000008 DACCQI010000000
VERSION     DACCQI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.008"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            147..1016
                     /locus_tag="IVO76_000334"
     CDS             147..1016
                     /locus_tag="IVO76_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7428328.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="IVO76_000335"
     CDS             1225..2250
                     /locus_tag="IVO76_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7428329.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="IVO76_000336"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="IVO76_000336"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7428330.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3333..5036
                     /locus_tag="IVO76_000337"
     CDS             3333..5036
                     /locus_tag="IVO76_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7428331.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="IVO76_000338"
     CDS             5038..7071
                     /locus_tag="IVO76_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7428332.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="IVO76_000339"
     CDS             7082..7522
                     /locus_tag="IVO76_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7428333.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="IVO76_000340"
     CDS             complement(7722..8849)
                     /locus_tag="IVO76_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428334.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="IVO76_000341"
     CDS             complement(8852..9529)
                     /locus_tag="IVO76_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428335.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="IVO76_000342"
     CDS             9793..11559
                     /locus_tag="IVO76_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7428336.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="IVO76_000343"
     CDS             11574..12011
                     /locus_tag="IVO76_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7428337.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="IVO76_000344"
     CDS             12024..13175
                     /locus_tag="IVO76_000344"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7428338.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13237..13935)
                     /locus_tag="IVO76_000345"
     CDS             complement(13237..13935)
                     /locus_tag="IVO76_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7428339.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13928..15280)
                     /locus_tag="IVO76_000346"
     CDS             complement(13928..15280)
                     /locus_tag="IVO76_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7428340.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15503..16903)
                     /locus_tag="IVO76_000347"
     CDS             complement(15503..16903)
                     /locus_tag="IVO76_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7428341.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17064..17801
                     /locus_tag="IVO76_000348"
     CDS             17064..17801
                     /locus_tag="IVO76_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7428342.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17926..18405
                     /locus_tag="IVO76_000349"
     CDS             17926..18405
                     /locus_tag="IVO76_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7428343.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18402..19169
                     /locus_tag="IVO76_000350"
     CDS             18402..19169
                     /locus_tag="IVO76_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428344.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19399..19968
                     /gene="lepB"
                     /locus_tag="IVO76_000351"
     CDS             19399..19968
                     /gene="lepB"
                     /locus_tag="IVO76_000351"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7428345.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20072..20674)
                     /locus_tag="IVO76_000352"
     CDS             complement(20072..20674)
                     /locus_tag="IVO76_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7428346.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20780..21249
                     /locus_tag="IVO76_000353"
                     /pseudo
     CDS             20780..21249
                     /locus_tag="IVO76_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21355..21939
                     /locus_tag="IVO76_000354"
     CDS             21355..21939
                     /locus_tag="IVO76_000354"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7428347.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21942..23015
                     /gene="prfA"
                     /locus_tag="IVO76_000355"
     CDS             21942..23015
                     /gene="prfA"
                     /locus_tag="IVO76_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7428348.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23008..23847
                     /gene="prmC"
                     /locus_tag="IVO76_000356"
     CDS             23008..23847
                     /gene="prmC"
                     /locus_tag="IVO76_000356"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7428349.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23840..24019
                     /locus_tag="IVO76_000357"
     CDS             23840..24019
                     /locus_tag="IVO76_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428350.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24041..25066
                     /locus_tag="IVO76_000358"
     CDS             24041..25066
                     /locus_tag="IVO76_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7428351.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25241..26485
                     /locus_tag="IVO76_000359"
     CDS             25241..26485
                     /locus_tag="IVO76_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7428352.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26638..27585
                     /locus_tag="IVO76_000360"
     CDS             26638..27585
                     /locus_tag="IVO76_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7428353.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27719..28348
                     /gene="upp"
                     /locus_tag="IVO76_000361"
     CDS             27719..28348
                     /gene="upp"
                     /locus_tag="IVO76_000361"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7428354.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28487..28729)
                     /locus_tag="IVO76_000362"
     CDS             complement(28487..28729)
                     /locus_tag="IVO76_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428355.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28850..28922
                     /locus_tag="IVO76_000363"
     tRNA            28850..28922
                     /locus_tag="IVO76_000363"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28883..28885,aa:Thr,seq:cgt)
     gene            complement(29041..29679)
                     /locus_tag="IVO76_000364"
     CDS             complement(29041..29679)
                     /locus_tag="IVO76_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7428356.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29802..30434)
                     /locus_tag="IVO76_000365"
     CDS             complement(29802..30434)
                     /locus_tag="IVO76_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7428357.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30785..31084
                     /locus_tag="IVO76_000366"
     CDS             30785..31084
                     /locus_tag="IVO76_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7428358.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31579..33744
                     /gene="acm"
                     /locus_tag="IVO76_000367"
     CDS             31579..33744
                     /gene="acm"
                     /locus_tag="IVO76_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP7428359.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(34016..34501)
                     /locus_tag="IVO76_000368"
     CDS             complement(34016..34501)
                     /locus_tag="IVO76_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7428360.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            34981..35622
                     /locus_tag="IVO76_000369"
     CDS             34981..35622
                     /locus_tag="IVO76_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7428361.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            35641..36276
                     /locus_tag="IVO76_000370"
     CDS             35641..36276
                     /locus_tag="IVO76_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7428362.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            36294..37109
                     /locus_tag="IVO76_000371"
     CDS             36294..37109
                     /locus_tag="IVO76_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7428363.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT        12802 a         6386 c         7510 g        10548 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gtctgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt ctggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccatgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaacgtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg acgaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcggaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggcag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaagag
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttcgct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata
    13261 gggcagatcc aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc
    13321 caggttctca tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta
    13381 aatgacacct tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa
    13441 aggtcgttca ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt
    13501 ttcattgaat tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact
    13561 taacgtgtag tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta
    13621 gtagtgacct agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat
    13681 atagttgatc aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa
    13741 gtcttgacca ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat
    13801 actaacgatc tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa
    13861 aaggtttccg ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag
    13921 ttcataatta gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac
    13981 gaagctgcaa tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt
    14041 tgattttttc gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc
    14101 cggcaacttt tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg
    14161 ggatatccat atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc
    14221 cgtctgcgta attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa
    14281 tcatatcgat tacttgattg atacctacag gatttttgac aagtactaat gtacattttt
    14341 tcccattgat attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt
    14401 gtttgatttt atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat
    14461 tgtagacatt atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat
    14521 ctgctgacac attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt
    14581 tgaagtcaca atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat
    14641 gcaagatgtg atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt
    14701 cacgatcttc ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat
    14761 tgaagattgg tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt
    14821 ctacaatcat tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa
    14881 tatttgtaaa taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt
    14941 catctatttc taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag
    15001 tcgatacaat tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt
    15061 gctggcgcaa aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga
    15121 caacgacgac ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg
    15181 ctagtttacc tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg
    15241 tttttccaac tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga
    15301 tttgctgacg aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt
    15361 ttcttattct taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga
    15421 tttaacaaaa aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt
    15481 ccctcttacc tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc
    15541 ctagatctcg tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc
    15601 caataaacat cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc
    15661 ctaaagataa accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg
    15721 tttgttcttc tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca
    15781 taaagacaac gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga
    15841 tgttatcttc acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg
    15901 ttcgaatccc gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa
    15961 tgatcagtgt tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag
    16021 tcgtaatcga ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata
    16081 aatggtcctt ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag
    16141 ctaacacagc taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt
    16201 tgtataatag ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa
    16261 tcaaaaacat gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc
    16321 cagtgacgtc aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa
    16381 cggcatcacg ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc
    16441 caaaaagtaa ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac
    16501 cagacaaacg cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc
    16561 gtcgtttaga aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt
    16621 gaaataatat ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag
    16681 tcgatagtcc agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa
    16741 catggctatc tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca
    16801 tcaatatata ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct
    16861 gtcctcgccg ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac
    16921 aatagtcgtt tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca
    16981 gataacaata cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa
    17041 aaagaaggag gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa
    17101 atcaaaaaat aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag
    17161 aagtaaagcc taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg
    17221 aattcatgtc tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta
    17281 aaagtgagta tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga
    17341 aaactgacca tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa
    17401 tggttttaac aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct
    17461 tcaatatgcg attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg
    17521 gaagtgccct gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg
    17581 atatccaaaa gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct
    17641 atgaacgctc tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt
    17701 gcgtgttcgc tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag
    17761 cttacttaaa aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac
    17821 gtaagaaaaa aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag
    17881 taaagtaagg aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca
    17941 cctattttga agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt
    18001 attgtgaata gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca
    18061 acaccaacta cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc
    18121 actacgacaa gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca
    18181 actgacagct caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc
    18241 aatccaagta caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact
    18301 gtagatgata acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca
    18361 ggaaataccc aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc
    18421 ttatcaacgg ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc
    18481 gtggatatac gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac
    18541 tttggcaaaa ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg
    18601 tttattttac tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg
    18661 tcactcagct ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat
    18721 cgatagataa tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg
    18781 acgaagatta tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga
    18841 tcaaaacaaa tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc
    18901 cttattacta tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct
    18961 actcggacat ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac
    19021 ctgtcggatt gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc
    19081 cttatcgtct gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata
    19141 gtcgtagcca aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg
    19201 ataaaaacgt ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt
    19261 atataacgag aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt
    19321 gtagaataaa gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat
    19381 acaagtagga ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta
    19441 ttagcggcta ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat
    19501 tcgatggacc ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat
    19561 gaacgaatgg atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa
    19621 cggatcatcg gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat
    19681 ggcaaaaagt atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag
    19741 ttacaaggag aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc
    19801 tttacgagtg actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac
    19861 cgcttgattt caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga
    19921 aaagttgttt tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa
    19981 aagaagctgt gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct
    20041 tggagttgct cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa
    20101 gcgaagcgtt tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag
    20161 agacaaagcc cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga
    20221 ctttcggatg actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag
    20281 gagctaaaat ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta
    20341 caagtgatag aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt
    20401 ggatcgttcg gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca
    20461 tagtttctga agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa
    20521 caagcccagt tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat
    20581 aagtagttag ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat
    20641 ggttagcgat ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg
    20701 aaattttaac ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa
    20761 gggagaatct agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa
    20821 gtgtgtccga gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga
    20881 aaaatatatc gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata
    20941 tttaggcttt atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt
    21001 atgggaataa aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca
    21061 gaaggttacg cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac
    21121 tactctatat atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa
    21181 gtttttccaa atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa
    21241 caaaagtgag caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa
    21301 atgctgaaaa tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca
    21361 caactatttt ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc
    21421 gcacataatt atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca
    21481 agagatggtt taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata
    21541 tttgaagaaa cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg
    21601 ctgatcgatg aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg
    21661 gacgaattgg atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta
    21721 tttgaaggat caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc
    21781 tgttggttct gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt
    21841 tatgaaggcg atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa
    21901 cattactttc acccatcaat gattacagaa ggagaataag catgtacgat caattacaag
    21961 caattgaaga ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg
    22021 atacaaagcg ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag
    22081 tttatcgtcg ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg
    22141 aaaaactgga tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag
    22201 aaaaagaagt cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg
    22261 ataaaaatat catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg
    22321 ctggcgatct tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag
    22381 tgatggaagc taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg
    22441 gagacaacgt atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc
    22501 cttcaactga atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag
    22561 aagctgaaga agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg
    22621 cttcaggtgc tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact
    22681 taccaacagg gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa
    22741 aagcaatgaa aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg
    22801 agtatgatgc caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga
    22861 cgtataattt cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat
    22921 tagatcagat tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc
    22981 aaacatcaaa actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca
    23041 cgggcttctt cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt
    23101 ttggaacgaa aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca
    23161 gaagaagaac agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct
    23221 cagtatttgc taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca
    23281 ctgatccctc gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt
    23341 gtaccgttag aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa
    23401 gcagcaagaa aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta
    23461 gcgaaagaaa atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta
    23521 ggaccagtaa tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg
    23581 aatgaatggg aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt
    23641 gctgagaacg atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta
    23701 aaatcaaatg gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa
    23761 atatttcagc aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat
    23821 gaacgaatga tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt
    23881 tattataaaa aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt
    23941 ttatccacta ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag
    24001 acaaaataag aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga
    24061 tgaaacagaa ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc
    24121 taccgaaaca gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt
    24181 gttttctgta aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga
    24241 acaagtaaaa gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt
    24301 ctggcctgga ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt
    24361 gacgggagga ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt
    24421 aatcgaactt tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag
    24481 tccaacgact gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga
    24541 tgatggagct acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc
    24601 catgcctatg attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga
    24661 atctcctgtt gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg
    24721 gatgaaatac aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg
    24781 gtcaactgca gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc
    24841 cgaaattgcg gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc
    24901 agtagaagcg gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact
    24961 tggattagat ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat
    25021 gaatcgcttg aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg
    25081 tcgcattctt ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa
    25141 ctaagcgtaa ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg
    25201 aaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt
    25261 tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga
    25321 gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt
    25381 gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt
    25441 cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc
    25501 gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga
    25561 accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc
    25621 accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac
    25681 agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat
    25741 cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc
    25801 agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc
    25861 aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac
    25921 attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat
    25981 aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa
    26041 agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat
    26101 cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc
    26161 aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa
    26221 agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt
    26281 tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac
    26341 tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca
    26401 agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga
    26461 aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa
    26521 tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct
    26581 ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg
    26641 actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat
    26701 ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa
    26761 tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg
    26821 ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt
    26881 ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc
    26941 agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca
    27001 gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa
    27061 ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt
    27121 ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc
    27181 aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa
    27241 ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg
    27301 acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa
    27361 ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa
    27421 ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc
    27481 caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt
    27541 gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc
    27601 aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa
    27661 tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat
    27721 gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga
    27781 caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc
    27841 atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga
    27901 aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg
    27961 tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg
    28021 tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga
    28081 cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat
    28141 catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt
    28201 agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac
    28261 agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc
    28321 gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt
    28381 gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc
    28441 gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta
    28501 gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg
    28561 cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt
    28621 gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt
    28681 ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct
    28741 ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc
    28801 ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc
    28861 tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg
    28921 cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt
    28981 gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat
    29041 ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca
    29101 acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc
    29161 ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta
    29221 atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact
    29281 agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa
    29341 aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt
    29401 caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata
    29461 tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt
    29521 accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat
    29581 ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc
    29641 ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca
    29701 gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga
    29761 tatttgtatt gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt
    29821 aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt
    29881 tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca
    29941 gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa
    30001 cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt
    30061 gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa
    30121 ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg
    30181 ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc
    30241 attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt
    30301 ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca
    30361 aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact
    30421 ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa
    30481 ccaacttaca ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg
    30541 ctatctcttg aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat
    30601 aaaaatctta gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc
    30661 gttgccataa cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag
    30721 atgtagcgta tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat
    30781 cgtgatgtct aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt
    30841 aaatccagag atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga
    30901 aaaaggtcag tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc
    30961 aatcagggga gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg
    31021 aaaatttgaa aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt
    31081 ataaaaacga gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc
    31141 taggttcatt tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt
    31201 tctttatatt gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg
    31261 ttaattaaaa gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt
    31321 taaaaattgg aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat
    31381 gcgaaaaaat aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt
    31441 ttttataaat atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg
    31501 gggaggaatt gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa
    31561 ataggggaga tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt
    31621 attactaata taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc
    31681 agcagtaatg tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata
    31741 aaagtaaaat tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg
    31801 atcaattggc caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta
    31861 atgattgaaa ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg
    31921 tttaatgaaa atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag
    31981 gccagaaacg taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt
    32041 gataaaacag ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt
    32101 tattataaaa caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat
    32161 attaacaata acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt
    32221 ggacaaagat tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag
    32281 gtttatagag gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt
    32341 aatttttcag taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt
    32401 aggaaaataa tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag
    32461 aatcattcgg aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc
    32521 aatcatacag ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa
    32581 ttgaagattt tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa
    32641 ttaagacgag cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat
    32701 gaacagggag aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa
    32761 gaagcaccca attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata
    32821 aacgaaaatg atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac
    32881 atcacagcaa ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa
    32941 ttattcagag caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta
    33001 gacaatggaa taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac
    33061 gaatatacgt ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt
    33121 tatcaaaaaa ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc
    33181 tcaggacaaa aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc
    33241 acagtaaatc tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat
    33301 gattggcgct atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat
    33361 actgtaacgg aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa
    33421 aacagttata caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg
    33481 aaaaattttc cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg
    33541 caaatatttg ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga
    33601 gctgtaccta aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa
    33661 aaatctagtt ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc
    33721 ttacaaaaat ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa
    33781 tcaaaagaat aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt
    33841 ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt
    33901 tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt
    33961 gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca
    34021 aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat
    34081 ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa
    34141 tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag
    34201 tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata
    34261 agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca
    34321 agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg
    34381 gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt
    34441 gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca
    34501 tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa
    34561 taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt
    34621 aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat
    34681 cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg
    34741 gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg
    34801 gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa
    34861 cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt
    34921 ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag
    34981 atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa
    35041 ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg
    35101 tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga
    35161 acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt
    35221 tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca
    35281 gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt
    35341 ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt
    35401 gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat
    35461 atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact
    35521 ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc
    35581 gtaatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat
    35641 atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg
    35701 aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa
    35761 accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta
    35821 gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta
    35881 ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca
    35941 aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct
    36001 tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa
    36061 cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa
    36121 ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg
    36181 aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc
    36241 ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa
    36301 aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca
    36361 aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt
    36421 tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg
    36481 atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca
    36541 ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg
    36601 gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa
    36661 acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata
    36721 ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag
    36781 aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag
    36841 cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag
    36901 actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg
    36961 agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca
    37021 atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt
    37081 ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca
    37141 cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa
    37201 tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagttt
//
LOCUS       DACCQI010000009        35199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCQI010000009 DACCQI010000000
VERSION     DACCQI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.009"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..962)
                     /locus_tag="IVO76_000372"
     CDS             complement(<1..962)
                     /locus_tag="IVO76_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7428364.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            complement(1078..1713)
                     /locus_tag="IVO76_000373"
     CDS             complement(1078..1713)
                     /locus_tag="IVO76_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP7428365.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLILLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTAKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            complement(1796..2218)
                     /locus_tag="IVO76_000374"
     CDS             complement(1796..2218)
                     /locus_tag="IVO76_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7428366.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(2236..2556)
                     /locus_tag="IVO76_000375"
     CDS             complement(2236..2556)
                     /locus_tag="IVO76_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7428367.1"
                     /translation="MNPYEKIKELTKQNGISVRELEKRLGYSNGYFSKWKSVSPNSEG
                     LAKVSDYFGVSIDYLLGRDKKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2856..3593
                     /locus_tag="IVO76_000376"
     CDS             2856..3593
                     /locus_tag="IVO76_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7428368.1"
                     /translation="MNTPQIFNFEQNEVRTFLENDIPYFVANDVAKTLGYKNPSDATN
                     KHCKKAVKTWGSDSLGRRQSFKVIPESDVYRLIIKSNLPSAEKFEAWVMEEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLE"
     gene            3617..3820
                     /locus_tag="IVO76_000377"
     CDS             3617..3820
                     /locus_tag="IVO76_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7428369.1"
                     /translation="MTDIAEITQRDREKIKAYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            3836..4156
                     /locus_tag="IVO76_000378"
     CDS             3836..4156
                     /locus_tag="IVO76_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7428370.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRR"
     gene            4161..4340
                     /locus_tag="IVO76_000379"
     CDS             4161..4340
                     /locus_tag="IVO76_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428371.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYRAKKASREGLTR"
     gene            complement(4460..5038)
                     /locus_tag="IVO76_000380"
     CDS             complement(4460..5038)
                     /locus_tag="IVO76_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428372.1"
                     /translation="MVVFSMNYILYVLLVVYAIIYIKQIVNAVSEITTINYLLTTIEK
                     VINDLKRLNDHSGSYKSDPYFQNNVTPIREIMVKEMPRINKVLPYSLVTLSLNQSDDI
                     LEENFRIIASQIFDKNNEIHYRKFWFLNPLIALKKFFLLPSEILSWFGINLSTFPGRI
                     FSLVIWVTSLFAQETISQILSFVITHFFKGQK"
     gene            5077..5775
                     /locus_tag="IVO76_000381"
     CDS             5077..5775
                     /locus_tag="IVO76_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7428373.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKNTMRISGQQEFEIKQKGNMKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            5957..6292
                     /locus_tag="IVO76_000382"
     CDS             5957..6292
                     /locus_tag="IVO76_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428374.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            6525..7466
                     /locus_tag="IVO76_000383"
     CDS             6525..7466
                     /locus_tag="IVO76_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7428375.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSEATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            7468..8358
                     /locus_tag="IVO76_000384"
     CDS             7468..8358
                     /locus_tag="IVO76_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAP7428376.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            8401..9201
                     /locus_tag="IVO76_000385"
     CDS             8401..9201
                     /locus_tag="IVO76_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP7428377.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            9216..10067
                     /locus_tag="IVO76_000386"
     CDS             9216..10067
                     /locus_tag="IVO76_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7428378.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            10064..10381
                     /locus_tag="IVO76_000387"
     CDS             10064..10381
                     /locus_tag="IVO76_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428379.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            10374..10556
                     /locus_tag="IVO76_000388"
     CDS             10374..10556
                     /locus_tag="IVO76_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428380.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            10564..10950
                     /locus_tag="IVO76_000389"
     CDS             10564..10950
                     /locus_tag="IVO76_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428381.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVGIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRV
                     FKHKKMDYEYEVIGNIYENSELLEVE"
     gene            10947..11282
                     /locus_tag="IVO76_000390"
     CDS             10947..11282
                     /locus_tag="IVO76_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428382.1"
                     /translation="MIILATDYIDSLKDEDGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSESAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            11282..11512
                     /locus_tag="IVO76_000391"
     CDS             11282..11512
                     /locus_tag="IVO76_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428383.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLSDGTEIVIRQSYLKDMAAP
                     VLIDRFNVTDSAVENHLKEFRSSIDDTFRLGS"
     gene            11519..11716
                     /locus_tag="IVO76_000392"
     CDS             11519..11716
                     /locus_tag="IVO76_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7428384.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            11713..11919
                     /locus_tag="IVO76_000393"
     CDS             11713..11919
                     /locus_tag="IVO76_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428385.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLSIVDILQSAKWFDNLEEAN
                     QVSRRLGGLTQVYELITIDHEEVE"
     gene            11919..12212
                     /locus_tag="IVO76_000394"
     CDS             11919..12212
                     /locus_tag="IVO76_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428386.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDKWLAKSKENLAVVRLVDMEVIKS"
     gene            12242..12682
                     /locus_tag="IVO76_000395"
     CDS             12242..12682
                     /locus_tag="IVO76_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7428387.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            12757..13224
                     /locus_tag="IVO76_000396"
     CDS             12757..13224
                     /locus_tag="IVO76_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAP7428388.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(13637..13951)
                     /locus_tag="IVO76_000397"
     CDS             complement(13637..13951)
                     /locus_tag="IVO76_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428389.1"
                     /translation="MSNGFDDLSKKLNKMAKAAQEMDGEHSVPMSELFTNDFVSANTK
                     FSSIEEFFEVSGFDCSSEESFAAIPDDQMDAFVSDNTNFDTWSDFMSAAAIKYTAKKL
                     GF"
     gene            14943..15287
                     /locus_tag="IVO76_000398"
     CDS             14943..15287
                     /locus_tag="IVO76_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7428390.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            15292..15573
                     /locus_tag="IVO76_000399"
     CDS             15292..15573
                     /locus_tag="IVO76_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428391.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            15675..15989
                     /locus_tag="IVO76_000400"
     CDS             15675..15989
                     /locus_tag="IVO76_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7428392.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            15967..17661
                     /locus_tag="IVO76_000401"
     CDS             15967..17661
                     /locus_tag="IVO76_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7428393.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            17681..18859
                     /locus_tag="IVO76_000402"
     CDS             17681..18859
                     /locus_tag="IVO76_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7428394.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKDFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            18822..19508
                     /locus_tag="IVO76_000403"
     CDS             18822..19508
                     /locus_tag="IVO76_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7428395.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            19508..20668
                     /locus_tag="IVO76_000404"
     CDS             19508..20668
                     /locus_tag="IVO76_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7428396.1"
                     /translation="MTIKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            20678..21553
                     /locus_tag="IVO76_000405"
     CDS             20678..21553
                     /locus_tag="IVO76_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428397.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            21550..21861
                     /locus_tag="IVO76_000406"
     CDS             21550..21861
                     /locus_tag="IVO76_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428398.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            21851..22204
                     /locus_tag="IVO76_000407"
     CDS             21851..22204
                     /locus_tag="IVO76_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7428399.1"
                     /translation="MANNRRLEETFNDGWLKILTQSTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            22194..22595
                     /locus_tag="IVO76_000408"
     CDS             22194..22595
                     /locus_tag="IVO76_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428400.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            22588..22992
                     /locus_tag="IVO76_000409"
     CDS             22588..22992
                     /locus_tag="IVO76_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428401.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            23004..23612
                     /locus_tag="IVO76_000410"
     CDS             23004..23612
                     /locus_tag="IVO76_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7428402.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            23632..23994
                     /locus_tag="IVO76_000411"
     CDS             23632..23994
                     /locus_tag="IVO76_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428403.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMSM
                     GKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDMEVAVAVYSTMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            23997..24179
                     /locus_tag="IVO76_000412"
     CDS             23997..24179
                     /locus_tag="IVO76_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428404.1"
                     /translation="MPNLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            24196..27516
                     /locus_tag="IVO76_000413"
     CDS             24196..27516
                     /locus_tag="IVO76_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7428405.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALDMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEKFGMLPNTIKPVFTQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFKGIWSKF
                     TDWLGGTWESIKEGASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWTSISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGIWN
                     SISSTASNIWSSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNGIT
                     STISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKI
                     SGIFDNLNISIPHIPLPHFKLSGKFNPLKGKIPTLGVDWYAKGSVFNSPNIIGIGEAG
                     PEVALPLKRSVLQEIGDRILSSTSVSSRAQTVQPVNNYEFNFTIDGNADEVTMKQTTQ
                     QIIDSITKVQNDNASAWR"
     gene            27567..28304
                     /locus_tag="IVO76_000414"
     CDS             27567..28304
                     /locus_tag="IVO76_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7428406.1"
                     /translation="MTDCIHSIVDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            28314..30605
                     /locus_tag="IVO76_000415"
     CDS             28314..30605
                     /locus_tag="IVO76_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428407.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIIEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWILVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            30629..32827
                     /locus_tag="IVO76_000416"
     CDS             30629..32827
                     /locus_tag="IVO76_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428408.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVITLDPNHKLEVLKPKSQPALLTGKTYTVSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSELYTRNTLTATKGVWQKLTGTVKITAASDNAESWLIMLQNKDA
                     NNSLSGKLRLRHAKLEEGSTATPFQPNLLAEPYNMCREYPNENIADPTVKFPIESGDH
                     QIYQGYTEEELMIGQTYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTF
                     TPTNVVAMPKLFRVYQYPRSTVGACRIDWLKIEKGNTRTPNISEYKYRGTGMRDSNNP
                     KDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSKINSQLTASINEVDTTAKGA
                     QTKANANATAIDELDNKIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTGYTTTAVG
                     ETHFERQSNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDNLVSYGVD
                     ALQNRFAASGNIVTDLEVNDVLNMRTEIGVANGILQFTSCRTLVLTRLSD"
     gene            32905..33147
                     /locus_tag="IVO76_000417"
     CDS             32905..33147
                     /locus_tag="IVO76_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7428409.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            33163..33360
                     /locus_tag="IVO76_000418"
     CDS             33163..33360
                     /locus_tag="IVO76_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7428410.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASALVAMLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            33371..34390
                     /locus_tag="IVO76_000419"
     CDS             33371..34390
                     /locus_tag="IVO76_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7428411.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGLNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            34580..34653
                     /locus_tag="IVO76_000420"
     tRNA            34580..34653
                     /locus_tag="IVO76_000420"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34614..34616,aa:Ile,seq:cat)
BASE COUNT        12403 a         5463 c         7178 g        10155 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttaac ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca attgaaattt tccgatttga agtctcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagta
      361 gcaccagcaa ctaacacaag cataggagaa gagtactgtg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttaattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgatgc tctaatatgg ttattaaact taataacggt ggattttaca
      601 tggtctttcg catacttatt taaaaagcgt tgatatccaa ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttcgccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ctactgtata
     1021 ggtttgtttt ttcatagaac acgcttgctt tggacggtgg gcgtgttttt atatcaatta
     1081 gttatgaaga gcaattgaaa ctgtctctgt tccaaagtaa cctgtttgtg cttgcaatac
     1141 attattttgt gcatttgctt gggcttcaga gatatcaaaa actattttac cagtttgttg
     1201 catatcagga tttaagcttt ccataaagaa agagttagtg atattaccat tttcatcttg
     1261 atttgccgac atagatgcag ccgaatcagc ttcgaaagtt tttccatctg cttttaattt
     1321 gaagaatgag ctatcaacag tcacagcctt atcacctgca ttttttacag ataagtcaac
     1381 tactaagaaa gtgtcttttg cgttagtagg aagtacagat gggccaactt gttttactac
     1441 ttcaacggag tttactttgt attccatttt ccctacagca acgtcatcac cgattttata
     1501 agtcttttct tcttttgctg tagtttcttt tgtagtttct ttattagtac ttgttgatgt
     1561 tgcagtagct gaatctttgt tttctccgcc acttagagca ccaccaatac caaaaaacaa
     1621 aagtaatatt aatacccaaa accacacttt tttgtaaaaa ggtttactaa ctttatacat
     1681 tttcccatct tgacccataa ctttttttgc catttaaata ttcctcattt cttgttataa
     1741 tatatttgtg atctcagaaa tgaggtatga gtccgtgttg cagcacgggc tttttttact
     1801 gtgcataaga gtattttttc ttgaaataag actggcaaac attaaaacat tctgttctta
     1861 acttattatt gatagaatag aactccatga aattttctaa tttgaactga gattcatcag
     1921 tcagttcgtt ctcaataaag atatttagta aaatcataat tgcgattcta tcagcttcag
     1981 tttcgaattt tgaatgaaaa gttgtagagt tatcgtacag tactgaatat tcaaaatgtg
     2041 aagcaatgaa atgaccgagc tcgtgggcta aatgaaaagc ttcagaactg tcttcgtgta
     2101 gtttttcatt cagaaatact attcttggtt ttggataata aaaacctggt tcttccattt
     2161 ccatatagat taactttaaa ttatactcac tcagcatttc tttcaacttt aaatacatac
     2221 aacccatcac tccaactatt cattttcctc taaagcttta gcaattgcaa tcgctttacg
     2281 cattgtctcc ttagatattt cttttccatc aaaagaaaaa acagtatcgt cttctgataa
     2341 atccacatgt ttaggggttt cttttttatc tcttcctaga agatagtcta tagaaacacc
     2401 aaaataatct gatacttttg ccaaaccttc ggagttaggt gatactgatt tccatttact
     2461 gaaatatcca tttgaatatc ccaatctttt ttctaattcc cttacggata ttccgttttg
     2521 ctttgttaat tcttttattt tttcgtatgg attcattgat atgtcaacct ttctagactg
     2581 acaagaaaaa atatagaata aaactctatt tatctgttga caaattagag ttaaacctct
     2641 atacttgttc ttgtaaacaa atttaacaac taaaaagaca acaaaaaaca cttttgatta
     2701 ttaaacgcca accgccaaaa aagcttttaa aatcaatgtt tatatgtctt atttaactat
     2761 gtacaaagta tagaataaaa ctctatgctt tgtcaattga atttagaaaa aagttgttaa
     2821 atttgtttac gaatataaaa gaaaggaatg aaaaaatgaa cacaccgcaa atttttaatt
     2881 tcgaacaaaa cgaagttcgg acgtttctag aaaatgacat tccgtatttc gtagcaaatg
     2941 atgtcgctaa aacattagga tacaaaaacc cgagcgatgc tactaataaa cattgtaaaa
     3001 aagccgtaaa aacatggggt agcgattcgc taggtcgtcg ccaatctttc aaagttattc
     3061 cagaatcaga tgtttaccgc ttgattatca aatcgaactt accaagcgct gaaaaatttg
     3121 aggcttgggt aatggaagaa gtccttccaa caatcagaaa aacaggtagc tattcaaacg
     3181 tacctcaaag ttttgcacaa gcattgcgtt tagcagcaga tttagaagaa aagaaccaat
     3241 tactcgaaca acaaattgcc gagtacgaac caaagattag ctacttagat acgattcttt
     3301 catcgacaga tacggtagcg acttctcaaa ttgcagctga ttacggaatg tcggcaattg
     3361 ctctaaacaa attgcttaac gagttaggtg ttcaacataa agttagcgga caatggatac
     3421 tttaccgaaa acatatgaac caaggataca caaaatcgca cacaagtgag attccgaaag
     3481 ccgatggcgg cactaaagtt gtaatgaata ccaaatggac acagaaaggg cgagtgttta
     3541 tttacaactt attaatcgca gagggctatt accctcaaat ggatttagaa taaattggtt
     3601 agaaaggagt tttagtatga ctgatattgc agaaatcact caacgagata gagaaaaaat
     3661 caaagcatat gtcgaaagtt cgaagttctt aacttacacc atgcttgctg aaagatttgg
     3721 aattagtaaa agctacttat ctttaatttt aaacggtaaa aagacttctg cagaagcaaa
     3781 cagaattata gattcgatta tcactatgta cgaattgtaa agggaggaac agcaaatgca
     3841 atatctagaa gcgaaaatcc caattccaga aggctatgta attatctccc aagtggatta
     3901 tgaggagttg aaaaaagctg atgatactgg tagatggatg acgttgccag aagtactaga
     3961 acggattaac agaaaatatg attggtttac ttctagagtt ttaaagaacc caagatatag
     4021 aaatattatc gatatcgaaa aaaacaaaaa tggatttgtc tattatccag ttgaaggaag
     4081 agacacatat ctatttttaa gaagtaaaac acttgaattt ttagaaacaa atttttcgga
     4141 aatcttaagg aggtaagcgg atgggaaaat ttaatagagc attagtgttc agcgcacccc
     4201 taatcatcta cgctttagga ctttggggaa gcagacaagc attaatcggc acgatcgttt
     4261 acatggtttg gatttttatg gggcttgatg aagctgagta cagagcaaaa aaagccagtc
     4321 gggagggact gactagatga gaaacaaaaa aatatctcta acgctattaa ctatcatcac
     4381 gattgtcgta tcgcaactat tctttttaac tatattaaac gacatatcga tgtcggaccg
     4441 attgttggtt caatcagctt tatttctgac ctttgaaaaa atgggtaatt acgaaagata
     4501 gaatttgaga aattgtttct tgggcaaata acgatgttac ccaaattaca agactaaaaa
     4561 ttcttcctgg aaaagttgat agattaatgc cgaaccaact aagtatttcg ctgggtaaga
     4621 gaaaaaattt cttaagagca atcaacggat ttaaaaacca gaattttcga taatgaattt
     4681 cgttgttttt gtcaaaaatt tgagaagcga taattctaaa attttcttca agaatatcat
     4741 ctgattgatt caaggaaagc gttacaaggc tataaggcag aactttgtta attctgggca
     4801 tttccttaac cattatttct ctaataggcg tcacattatt ttgaaagtaa ggatcagatt
     4861 tatagctacc actatggtca tttaatcgtt tgaggtcatt tatcactttc tcaattgtag
     4921 taagtaaata gttaatcgtt gttatttcag acactgcatt aacaatttgc ttaatataaa
     4981 ttattgcata aacaactaga agcacataca aaatgtagtt cattgaaaac accaccattt
     5041 ttttacctaa attataccaa aaggagagaa gaaaaaatgc aagaattagt aattttgaaa
     5101 aataaagaag ctgtgactac gagcttacaa gtggcagaaa gctttgagaa aaaacatcaa
     5161 catgttttaa gagatattga tgcactaaaa aaagatgtgt ccaattttgg acagatgttt
     5221 gtagaaggta atgaaccaga ttcatacggc agaaatcgac gagttttttt cattagtaga
     5281 gatggttttt tcttgttggc tatgggtttt acaggaaaga aagctattta cttcaaacaa
     5341 aaatacattg aagcattcaa cgaaatggaa gatgttattc gcaagaatac tgttcctcaa
     5401 acaattgaag acatgatgat ctaccaacta gaagaaatga aagatgttaa aaaagatgtt
     5461 tccatgctta aaaatactat gcgaattagc ggacaacaag agtttgaaat taagcaaaaa
     5521 ggaaatatga aagttatgga agttctagga ggcaaagaaa gccgagctta tgaagaaatc
     5581 agtaaaaaag tattctcaaa attttggtct gaatttaaac gtaccttttc aatcccaaga
     5641 tatggcgagt tacctcgtaa aagattcgat gatgctgttt catttattga aatgtggtta
     5701 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag acttttcggt
     5761 gatgaaaatg aatagagctg aagcgctaag aatagggacg gtaattgcta atcgctggtg
     5821 gagacacaat aaaccaagca tcctaagcca acaacatatt gataagcaaa aagcttggca
     5881 acaaataaaa aagtgactca gccgtccaaa gcaatgagtc acaaaccaaa aattatacct
     5941 aaggagattt tatcacatga ataagaaaat tgaaaatctt atcgaagaat tgaaacgtga
     6001 atgccaaaag caaggtgttt ctattatttg tacagcccaa aaagaagggg aacttaaaag
     6061 tctcgtttat ggtgagacaa cagaaatttt actttgtctt gctatgcaag aagaacattt
     6121 agatgaaaat ctcccattgt cagcacatat tatgcgtaga attgctgtgg atgcttacga
     6181 acaagcgaaa aatgaagaag aaaatcaacc ttcaaatcat acttttgtta ttaataacaa
     6241 ggaagattta gctgatgtga tgactcgtat tctcaagggg gaatttcaat gatgaatgaa
     6301 aaaacaattg atctactcat tattttattt ttcacgttag tgattgtagc gaaaagttat
     6361 gtaatctttg gcttgttatt cgcaattacc atgcttgtgc tttgcgtgtt agcaagcaga
     6421 aaagaggtta aacatgaacg agttagcaaa tcttgacaac tatttaactg atcctgatta
     6481 tgctaaacca ccatatgaag cgccaattga tgaggaggat gaagatgagt aaatctacct
     6541 tagaaatgag ccatcaagaa tggctcgaag accgcaaaag aggtattggt ggctcagatg
     6601 ttgcaaccgt acttggatta aacaaataca aatctcctta tcaattgtgg cttgagaaaa
     6661 cgggtcaaat tgaattgaaa gatttagaaa gcgaaccagc ttattggggt aatgttttag
     6721 aagaagtggt tgctaaagag tttcaggaac gtacaggcaa aaaagtacgc agaagaaacc
     6781 aagtatttga acatccatta catccatttt taagagcaaa tattgatcgg gaagtagtgg
     6841 gagaaaatgc cattctggaa tgtaaaacag ccaatcaatt tctcggaaaa gagtgggaag
     6901 gtgaagaggt accgcttagc tatctctgtc aagttcagca ttacatgaac gttttaaaca
     6961 aagactattg ttatatcgct gtcttgatcg gtggccaaaa attcatctgg aagcggattg
     7021 aacgagatca agagctgatc gatacaatca ctgaacaatt agtagagttt tgggaaacga
     7081 acgttcttgg aggtatcgag cctgtaattg acggaagtga agcgactgct gacttcttaa
     7141 aagaaaaata tgcagatgta gaagaaaatc aaacagctct accatcacgt tttgatgaac
     7201 ttatcgagca aaaaagagaa ctcaagcgga cgaaaaaaga aattgaatca tctattcgtc
     7261 aagtagacaa cgagatcatc agtgagctag gtaaacgtga agccagtatc ggtatcacac
     7321 aaagaaacat tatcagctgg aaacttgttc gtacaagacg gatgaacacg aagaaactag
     7381 cagagaaata tccagatgtc gcaaatgatg aagagattta taacgttact gaatcaagaa
     7441 ggctaaccga aaaggagatc aaataatatg gcaacaaatg aatcgttaaa aaatcaattg
     7501 gcagaaaagc cacagaaaca agttgcacca ggacagttag ggcttaaagc tctaatgaat
     7561 acaccaacaa tgagaaagaa atttgaagaa gtacttcatg acaatgctaa tgcttttatg
     7621 tcgaatgtta tgactcttgt atctaatgac agttatcttg cagatagtga accgatgtct
     7681 atcatgagtg gtgcgttaac tgctgcaaca ttaaatcttg ggctagataa gaatttaggt
     7741 tatgcatatt tagttccatt caatagtaaa aacaagcaaa caggaaaatg ggaaaagaaa
     7801 gctcaattca tgcttggcta taaaggatat atccaattag cccaacgatc aggtaaatac
     7861 aaagcattaa atgtgatcga agtttacgaa ggagaactaa aaagctggaa ccgactgaca
     7921 gaagagtttg agtttgatcc aaatggtaga acgtctgatg aagtcattgg atatgttggc
     7981 tatttcgagt tactgaatgg attcaaaaaa actgtctatt ggaccaaaca agaaatcgaa
     8041 gctcatcgga ttgctaacaa taaagatcgc gataagacaa agttaagtgg tgtgtgggca
     8101 tctgattaca atgcaatggc acgaaaaact gttttgagaa atcttctttc taaatgggga
     8161 atcttgtcca tcgaaatgca agaagccacc acatcggatg agagagtcca aagggttcaa
     8221 gaagacggca gcattattgc tgaaacagaa gttgaagaag atattcctga aagaaaagaa
     8281 gcagaggtta tatctgaaga aaatgaagat gtacaaactg gattatttga tgcatctaat
     8341 ccgccgttaa acaaataatg agggagcttt ctcccttgct tttctagagg agaaatacga
     8401 atgaacacag gatatgtcaa attacatcga aaagtgatga attcattcgt ttggaccaat
     8461 ccttatatgt ataaattatg gagcttgtgt ttgatgaaag cgagtcatga gaatcgcaag
     8521 atacttttta atggaaaaga gatacaagtg aacagcggag aattcgtcac agggcgcgat
     8581 gctatcacat ctgagatgaa cgaaggcgtg aaacgtgaac atcaagtgaa cagcggttct
     8641 gtatggagat ggctaaaaca atttgaaaaa aacggaatgt tgaacatcaa atcaactacg
     8701 aaatacagcg ttatatcaat aaaaaactgg tctttgtacc aaagcagtga acaacaaatg
     8761 aacatcaaac gcacaacaag tgaacaacaa gtgcacacaa acaagaatga aaagaattat
     8821 aagaatgaaa agaataataa aacatcatcg tcgcaaccac gcaaaaagcg tgtttacgac
     8881 accgactcag tttactacat tctcgcggag gagttattca aacagatttg tcagaatcag
     8941 gaaatcaaaa agccgaatct gcaaagttgg gcagatgaca ttcggaaaat gatcgagatc
     9001 gacaagcgga ccgaaaacca ggtacgggga atgattgaat ggagtcagca caatgtgttt
     9061 tgggcatcga atattttatc tgctaaaaaa ttgcgagaaa aatacgacac aatggcagcg
     9121 caagcaaatc gtgattataa aacaaaacaa actaaaacgc ttgaatacga gaaatttagc
     9181 acagatgagt tgcctatttg agaggaggcg taggcatgga aactgttggg gaaatcatgg
     9241 aaaagctgat acagaaagtg cttgtccaac gtggcgaatg tcctgaatgc ggacagcctt
     9301 tgtatggatg gcgtacgaag aaccctgatg gctcagaacg ttgtaagcca acctgtatgc
     9361 aatgtgggta taaagcattg cgagttcagg aagacttaca aacagaacga atttacaacg
     9421 agagcctgaa agcaagagca atcaattttt tcaaaggtgg ttctgttgtg cctaatcaag
     9481 cgttgtttga ttgcacattg cagaattatc aaattgtcga tcaagaaaca agacaagcgg
     9541 ttgaagtaac caaacgcttt gttaattcag tcttgttagg aaatccaagt caccttgttt
     9601 taactggaaa acaaggaaca ggtaaaagtc acctagcgat ggcagcggct tgggaagtct
     9661 tgaagcgatc aaactacgat aaaaaaatct tatttatcgg gttacaggaa atgctggatc
     9721 aaatcaaatt ttcgtacaac aatcctgaac tcagaaaaac gattgaggga tcgttgattg
     9781 cagatatcaa aacagcggat ttagtcatca ttgatgatat cggttcagaa ctaggaaaag
     9841 atgcatcaga tagtcgagcg tttggcataa acacgctaaa ttcgttcttg gatgcacgac
     9901 agaacctagc aacgattatc acaacgaact tgcttggtga agaactgaaa aaagcttacg
     9961 gtacgagaac aatatcaaga atgtttgtca actctgatgg atttacgatg gtattttctc
    10021 aaacagcaga caagcgcata aaaccagtca aaggagcgat cgcatgaatt tgactaaaca
    10081 agaaaaaaga atctgtcgtc agataatgtt gcaaattata ggtgatcgtg acatggacga
    10141 attatctcaa caagagagtc gaatggttcg gaagataagt aagctaatcg gtgcagatac
    10201 gatggatcga tccaagccat tacccaaact gaattttgaa tcatttactt ttgagaaata
    10261 tagccatctg agagaggtag gctatagtgt aaatgctatt cgaaaagcac ttggggtagg
    10321 aagtagtgta tgggcaaaat ggcggaaaga aaatgtaaat gaggtaaatc aaaatgatta
    10381 agctaatagg caaagagacg ctcaaggttt actatgtagc ggaaacaaag gcagaagtca
    10441 gccgttggct attgaaaatg tttaataaga ctagagccaa tgcttcagta agtcagaaag
    10501 caagtgtatc agaaacaggc atgcctgaag caatgtggat ttcaaggagg aactgagatg
    10561 gcaatgatac cgaagtttag agcgtggtac acaccattta aaggtaaaac aattggacaa
    10621 gaaatgaaat atgggcaagc aggaaggttg atcactcatg ctgaaatggc tccagataaa
    10681 tatgtgctta tgcaatccac aggactgaaa gacaagaacg gtgtggggat atttgaaggg
    10741 gatgtagttc ttgtcaatgt aagtaatggt tttgatcatt tagttaatga aaaaacggtt
    10801 gtacaagaat ccgagtttca ttctggatta gtttgtaaat cattaattag tggaatggaa
    10861 tatagagtat tcaaacacaa aaagatggat tatgaatatg aagtcatcgg aaatatttac
    10921 gagaattcag agttattgga ggtagagtga ttatcttagc tactgactac atcgattctt
    10981 taaaagatga agatgggaat gtcccagcat tgctaaattg tggaattaaa agtaagaata
    11041 gaatcatttt gttacggata tcggctgatt tagcaaaata tctttatctg agcaagacag
    11101 cagtatatta cactgcggct acacgttatc agtacaaggg aaaaacaatc agtcaggatt
    11161 attatgatcg tttaatcagt cctgacatgc atggaaaatc agaatcagcg atcaagttat
    11221 ttggcgcaat agagatatat gccgatgatt ttccaaattt atggttgaag gaggaacagt
    11281 aatgaaatac gaaataccac taagcgaagc agatgttcag tcaattatta acggtcggga
    11341 ggttaataaa aaactttcag atggtactga aatagttatc agacaaagtt atttgaaaga
    11401 tatggcagct cctgtattaa ttgatcgttt taacgtgact gattctgcgg tagagaacca
    11461 cttaaaagaa tttcgatcaa gtatagacga cactttcaga ttagggagtt gattgacaat
    11521 gaacaacaga catcgcagaa taacaaaact aagaaaacgg gaactgaatg tactaaagac
    11581 aaagtttgaa aaagaatatg gaatttcagc agaagaaaca tataaagtgg caagtcagtg
    11641 tgttgctgat gcaagtgatg ctattcgtaa gtttggaatt tcgatactag atattaaatg
    11701 ggaggataca gaatgaagat ttatgtagta aagtttggaa ataaatttta cagaagtgat
    11761 gaacgttcta taggggctaa cacattatcc attgtagaca tactccaaag tgcaaaatgg
    11821 tttgataatc tcgaagaagc taaccaagtt tcacgacgac ttggtggatt aacgcaagta
    11881 tatgaactga tcactatcga tcatgaggag gtagagtaat gaaactaaaa gacggatttt
    11941 acgctagcag tcacggtatc ggcggtttaa tgctagatat gccgacaaag aatcctaaaa
    12001 cacgtaagaa accaaaagtc aaagtcggtg acatggttcg ctgcgaagca gaggagttcg
    12061 tttatccctt cagaggatac gttaaaaaga tactgtcaaa ctcagcaatc attcgtattg
    12121 aaaacacgat gaaatgtgac aagtggttag cgaaaagcaa agagaatcta gcagtggtga
    12181 gattggtgga tatggaagtt ataaaatctt aattttgctt ttttacaact atcctacaac
    12241 aatgagcgtt attccacaac tggaaggaga agaggcaatt gtgaacattt tagagaatat
    12301 agatattaag caaacaagga aaaatgctag acgattacta aaaagataca gaaatttaga
    12361 acgtttggtc ggaccagtga aaatagactt ttccgtgatg actgttacca aaaatttgaa
    12421 attcacaatt gacagtcaaa acgaagaaat cattgaagcg ataagtactc gagattcggt
    12481 tatcgaagca ttaacacggc taagtagaat ccatttccag gtgctttatt atagttattg
    12541 ttttcctaat aagatgtcaa tgtatcaaat aggagaaaaa ttaggatatt ccgatagaac
    12601 tatcgagaga atgaaggcag tagctttggt tgaatttgct gaggcgtata aatcaggaga
    12661 actcatttca cgtacaaaat aaaaaaagtc ggattcctcc gactatgaat aatatttccg
    12721 acataagtat tatatcataa ttgggggaat cagaggatgg tacttttcga tgtaaagaaa
    12781 tatgagacac caagcgcgaa agatgttgat atggaacgca caaaacataa tgtcgctgtg
    12841 tttctttcag catatctatc agctagatgt agagtagggc aacctcgaga accaaaagtg
    12901 acagcatctt attccttggt tccaccttct acagctgatc atatatttga agcagaaaga
    12961 atgctgatcg ataaagaaga agcacaggaa gaatttgagt atctgcataa attgtttgtt
    13021 cgaggctatt cagcgataca gcatccgcat aagcctgatg tgactgaaag gcgcaagaag
    13081 atattctatg atcgttatat caatggtctg cccatttatg taactgctca aaggaataat
    13141 actagcgaag aatcggttaa agtagaatca aacagaatta tcatccaatt tgcttcatcg
    13201 ttagaactgg ttgctttcaa gtagccagtt tttacacttt ttataccctt ttattaccaa
    13261 tttggtttcc attttatacc ttttttatac caatcactta cctattcaac gttgtattat
    13321 gatagtgtcg aaagattagg aaacaggatc gacaaaataa aatgtaaggg aggaaatctc
    13381 cctcatcgtt taattaagct tcgataggca gcaacggaaa tattaagaat aaggatgtga
    13441 atttcaactc cttctaaatt gttcttatta tctatcatcc gttgctgtct attaatttat
    13501 gtattggagg aaaaacgaat ggataaagaa atcaaagcaa ctgtcaaatt agatttgact
    13561 gaactaaaag aactgctcaa caaggctagt gaccaagtcg aacagttaca agaagcttta
    13621 gatgaaattg ctaattttaa aatccaagtt tcttagcagt atattttata gcagctgcac
    13681 tcatgaaatc agaccaagta tcaaaatttg tattatcaga tacgaatgca tccatttgat
    13741 catcaggaat cgcggcaaac gattcttcag agctgcagtc aaatccgcta acctcaaaga
    13801 attcctcaat ggagctgaat ttggtattag ctgaaacaaa atcatttgta aataactcgg
    13861 acattggaac gctatgttca ccgtccattt cttgggcagc ttttgccatt ttgttaagtt
    13921 ttttacttaa atcatcaaac ccgttactca tgttatcacc acctataatt tatttcagcg
    13981 gaccactcgc tgataaataa aattatacgc ttagtattta ttttcacaat attaatttat
    14041 cgtaataact tttgagtaaa tagaacaaaa aacctgcact agtttccgct agtacaggca
    14101 gtgactatat cggttgataa tctagcatat attaaaaata attgcaagaa aagaaattta
    14161 tagtggttta taccaaatta tcaaagcaat gttattttgt tgctgtctat tgttttttaa
    14221 ttattcacac gataactaaa ggtgggtgaa gagaaatgat tccattaata atttcaattt
    14281 ttgcgctctg tcttaatgtc tatatgattg gatttaaaaa tgggcaaaat aaaaaatagt
    14341 agcagccaag aataatttta tagtgtcgag tgtcactgtg gcggaaatag aagacacaac
    14401 ggtaaatgac gagtagcctc gtgagagcct agtaagttct cgtgtgtggt gcgattccac
    14461 tccagtgact ttgggtttgc ggtacaagat cccgaaagta aaaccgctag caaccgaggg
    14521 ttggaaatgg gcgctcaaag tacacgagca aggcgaggtc gatagtaatc gatggaatcg
    14581 gtgtaggttg ctattacata gttggttagg ttagattgag ttttgggatt tggtacaaat
    14641 gaatcgtcaa atgactcaag cacaggatcg gaaacgtccc tgcctgtgca ttacatatta
    14701 gatcactctt tgagtggtct ttttattttg aaaaaaggag gtaacaacaa tgtatagacc
    14761 acaatactta gaacagaagc atgaagtaat cactgtgcaa aacggtaacg gtgagatagt
    14821 acgaaagtat agaagaccaa taaagagcga tacatataaa cggaaggaaa gcaatgaagt
    14881 tattccattg tatggcaaaa gaatagctaa gtattaaata agattgcgaa aggagatggg
    14941 acatgaccga ggaattctat agatggttat tacagttgat aagagaagat cgtttggtta
    15001 agttctatca gtctcctaaa tggcgcaggc ttagagagaa agcgatgaaa cgagatcact
    15061 atgaatgcca agagtgtaga agactaggta agtatcatag agtagagaac gttcatcata
    15121 taaaggaagt caaggataga cctgacttag ctttagattt agataatctt atttgtttat
    15181 gtgttgaaca tcataatgaa gttcatggca gatatcttac agcattagat aaacaagaga
    15241 agaagataga aagctttgct aacttcgatg caagtgaaag gtggtaagtg catgatcatc
    15301 aatgacaatg gcagagagta tgatacagag aagattgaag agtattcatc ttatacacaa
    15361 ggattaatta aacgtttgat atacgttcgc tatgtaggta ttagggatct gttatcagat
    15421 aactgttgca gtaaatacaa agtgaatcaa gtaagagaag cgttgaataa agataataac
    15481 gttgaaagaa taaaaaatat ttttggatat agcattgaag agattaatta ttacattgac
    15541 ttcgctgaag ctttcattcc gatggtgaga taacccccct taaaataaat cgcaaatttt
    15601 ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg
    15661 aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac
    15721 gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca ttcttagata
    15781 atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa gatatatccg
    15841 agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg cttgtttctg
    15901 tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa ttggtaggtg
    15961 aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa agctatagaa
    16021 gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct agaagaaaga
    16081 gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc aatcaatata
    16141 ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca atgttttatt
    16201 tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct ttcattaatg
    16261 ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct actaaccgct
    16321 aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag ccaagcaaag
    16381 actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga taaaaaagta
    16441 tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact tcgttataac
    16501 acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt tgatgaaatt
    16561 cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg taaaattcga
    16621 gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc gcttgatgac
    16681 attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa ggatggagca
    16741 gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga agttgatgat
    16801 cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcagattt aaaacggaaa
    16861 atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac gttcatgacc
    16921 aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata tgatgatgtt
    16981 ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg aacagtcgat
    17041 tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga taaagatgtt
    17101 tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa catcaaacga
    17161 gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg aaaaaatata
    17221 gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta tattaaaaaa
    17281 atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt
    17341 actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga tctggttgat
    17401 gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg ttggtattgc
    17461 atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa aatagaacct
    17521 gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt tttagatgat
    17581 atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac aggaactgga
    17641 tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc
    17701 ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa gaatactttt gtaaattgca
    17761 agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga ttgcaaatgc
    17821 gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga atgatcttta
    17881 ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta gaaaaaaact
    17941 gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta ataattctag
    18001 cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag atgatgtgtt
    18061 ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa gtgatgttat
    18121 ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt atcaagcgta
    18181 tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt
    18241 actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa tcaataaaat
    18301 gatgacatca caatttaagg attttatgga agctgataat gcaggtgcgg tatttcaatt
    18361 acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt caagagatat
    18421 aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg ttccgaaaaa
    18481 cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt taatgttcga
    18541 aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct atgaagtaga
    18601 agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga ttactagctt
    18661 caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta caatcaacga
    18721 aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg aaatatttat
    18781 aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat
    18841 ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga tttattggtt
    18901 ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg gatagtctag
    18961 gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta tttcaaggga
    19021 ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg attaacggct
    19081 tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa atctacaaca
    19141 atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc gatgacttcc
    19201 gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca tataaaacac
    19261 gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg tttatggatg
    19321 cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa aatagctcag
    19381 gtactgaagc taaaaaagaa caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa
    19441 gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga caaggagatg
    19501 caaaataatg acaattaaaa atttaaaagg tgtaacagct gcaagcgacc aattgatgaa
    19561 agcttttaaa gatggtaacg aagaatcttt tagcgcagct atggtaagct tatctaagga
    19621 aattcaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat tagtattaat
    19681 gaaccgtggt cagcgtgtat taactacaca agaaacaaaa ttctataacg aagtagtgaa
    19741 aaacgaaggt tttgcagggg tcgaagaatt agtgccagct actgtatttg aacgtgtatt
    19801 tgaagattta gaacaatctc atccactatt gcaaaaaatt acttttgtta acacaactgg
    19861 tgtaacagaa tggattgtgt cacgtggagt caatccagca tggtggggta aactttgcga
    19921 agctgttaaa aaagttttag ataatggctt tgatgtaatt aacatgaagc agttcaagct
    19981 atcaggttat attcctgtat gtaaggcaat gcttgattta ggtccagtat ggttagatcg
    20041 ttatgtccgt actgttttag tagaatcatt gagaattgca ttagaacaag caattgttga
    20101 tggtactggt aaagatatgc cagtcggaat gatgcgtgac atgagcaaac aaactagcgg
    20161 agaatatgct gaaaaaaaag cagaacctat tacagcttta gatgctgtaa ctatgggcgg
    20221 tttgatggcg cgactatcaa aattcaatat cgaaggtgtg aatgatccga tttatcgtaa
    20281 tgtgaatcct tctgatgtgg tcctaattgt gaatccaact gattactggt ctaaagtatt
    20341 cccagctaag actgtactaa ctgctaatgg agaatacgta caagtattgc cagtaccagt
    20401 ttcagatttg cagtcaacgg ctgtgccaga aggaaaagca gttattgggg tagcctcaga
    20461 ttacttcatg ggtgtaggat ctacactaaa aattgaagct tcagatgaat accattttgt
    20521 tgaagacgaa cgcatttatc tagctaaaca atatgcaaac ggtcaaccta aacgtaacga
    20581 tagtttcatt gtattagata ttagcgcttt gggaactact actacaacta caaaaccaac
    20641 aaccacaaca actacaacac aagcgtaggt gatcagaatg aagtatattc tttgtcagcc
    20701 ggcaatcaat cggtttaaat gggagcttga agtttgttta actaatctga agaaactagg
    20761 aatcaaagat atcgtattgc ttttcagcag acacgatgat cagattccta ttttttttga
    20821 gaaggaatat ggtgttgaag ttcatgtgta cgatgatctg cgggacgaca aagagtatat
    20881 tccttcgatt aaaccatatt tatggtggaa atatttagaa gaagatcatt cgcgtgagga
    20941 cgaccgatat ttctatatcg attcggatgt cattttcaat aaaagaatta atttgcgcaa
    21001 attgccttct aaagatgatg tttggtattg tagcgactgc tgtagttatc taagtcttga
    21061 ttatattaga agctgtgaaa acggagaaaa tattctaaaa gatatggcaa acattgtaaa
    21121 tgttacagta gaatctttgg aaactataaa cactaattca ggaggcgcac agtgggttat
    21181 taaccgtcct aaagcgaatt attggaaaaa ggtttatctg gattctaatc ggctatatcg
    21241 ctaccttaga gggcaaaaaa caaatataca aatctggaca gccgagatgt gggcacagct
    21301 ttggaacatg atgtatttca atattggtcc taaagttcac gaggaattag acttttgttt
    21361 tgctactgat ccaatagaaa aagttaaaga agtaaaaatc ttacacaatg ctggagtaac
    21421 aacaaacgat gaagatttat ttttcaaagg gagatacgtg acttctacgc cttttgatga
    21481 agatttatca tttgtaaaca agaaaaaatg ctcttacgca tatgttaaag caattaaggc
    21541 ggtggttaga tgacgcctga acaagtgact gaagaattgc taatagctgt gaaggataat
    21601 atttacgtta cctggaacga agaagatgag tcaattaaaa agatgatagc taaaaatgct
    21661 gtttatcttc aaagtaaagt gagtacaaca ctttcttttt ctcctgaaag cttagaatac
    21721 ggattgctaa tcgaaagatg tagatacgac tggaatcgtg ctttagatga gtttgaacaa
    21781 aatttcgcta gtgagttatt aggtttcatt caacattatg cgctacaaga atatattgca
    21841 ggtgatgtga atggcgaata atcgtagact cgaagaaaca ttcaacgatg gttggttaaa
    21901 gattttgacg caaagtacca aaagaaatga actaggaaaa aagattggtg tagaagatac
    21961 agaaatcact tctttaaaat ttagaaatct ttccatgaga gatagtgata taacagctat
    22021 ggatgcgatg ggatcgaaat taactaagaa agtaaagact ccatttcatc caatcgccaa
    22081 gaaatttaat aaagatcaat attttatcgt aatcgatagt atgcgttaca acgttatcta
    22141 tgccgattac gataattttt atatctattt ttatcttgaa agtgtgggtg aatatggtga
    22201 ttgataattc taaagaaaaa gaacgtttaa ataagcaaat ttctgctatc aaaacttcct
    22261 tagaagagca ttttggcctc aaactctttc aagactccgt aggcgaggat gagctacctg
    22321 atgattttaa ttacttcatt ctcgaaacag gagaaataga aatgatcact gagccaaaat
    22381 atagcgtggg tcaaaatcta tatctaactt tctattcaga aaatagagaa gatttaacag
    22441 gagactcact agatattatt tcattgattc aaaatcgttc gattcgtttt cagagaatgg
    22501 atcccaatca tttaaaacta gagaaccaag atcgctatat cgatcaattg gtatttacgt
    22561 ttagacgatt attgaagagt gattgtcatg gctaaaaata gttgggagct aaaaataaat
    22621 ggacatgatg aacttcttgt gcggatggaa cgctattcaa gcgagagcga acgactgatt
    22681 aacgaagcat tgaaatcaaa aggttcggct attgcagtgg ataggattac agaaaaaatt
    22741 cctgtttctg aagcagattt aagaagaggg caccaacacg caaaaaatag tcgtccactt
    22801 aagactcaat acattaattt gggtttcatc attagaccta caagaaaatt tgagtattta
    22861 aaatatcctg atttggggat aggtacttct aaaagaaatc agccggacga atttatgaga
    22921 agaggattag gtcttgcact tgatccaatt acagaacttc tgattcgtca attcgataaa
    22981 ttaaataaat agggggaaca acaatggcta aaacaaaaac tgtagtaaca acgttcgata
    23041 acgtaagtat caaacgaatt gcttttaatt ttaagaacgc aggaaatgca atcgcaacga
    23101 attgtaacgg acaattagat ggcgaaacag aaatgcaaac ggtggttaaa aaatgtggag
    23161 cgacagaagt aaaatcaaaa tctaaaccaa tcaatatgac ggtaacaatt actgcacatg
    23221 taccgatgga agtttatcga cgtttcaatg ggttgaaaca agatggacgt attaaaccag
    23281 gcatttactc ttacggtcct gattccgtag gcgaagattt ctcacttgct gcagagatcg
    23341 tggatgactt cgaagaaaat agaaagttag ttggtatgtt agcatgcact tcgaatacag
    23401 gattaacatt ctctattgaa aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa
    23461 aagttatgca agatgaattt ggtaaatttt atcatgaagc aattgttgca gaacttgaag
    23521 aagacttaac agatcaatgg atgacgaatc tatctgctga tgtgattaaa aaaacttcag
    23581 ttgtgacaac tacggccact caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa
    23641 gattactcaa aaattgaact aaacgatgga acaattttga atttagaacc taaactgaat
    23701 atcaagaaat tattgatgat caatagagat tttaacacag acgagtttgc aaaaatgtcg
    23761 atgggaaaag gctctatgga cattacagtt attcaaggtg caaaagccgt atatgtagct
    23821 tatcgtcaag cgaatatggt tgattacatt tcattcgatg aatttatcga caaatgggat
    23881 tttgatatgg aggttgcagt agctgtatac agtactatga tgttcaaaca agcacgagat
    23941 gcctatcaaa aagaattcga aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaatgc
    24001 caaatctctt agttgaaacg tgggtcgatg tctattcgat gttgaccgac gttttttcta
    24061 tgccttcaga tttggtttta agcgatatct gtttagatga cattttgcaa atggcttaca
    24121 acaagagtgc ttatgaagga tggaaaaact atgcaataaa ccaatcccag aaaaactaaa
    24181 gaaaggaggt aaaaaatggc taaaaagaga acagaagcag aagtaacatt tatagctaac
    24241 gatgacggat tgaaatctac gttaaaagaa atcagcgctg aattaactaa aaatagagca
    24301 gaattaaaac tagaacaagc tcaattacaa cagactggtt ctgaatcaga caagttagga
    24361 agtaaattat cttctttaga gaagcagtat gaattacaaa gtcaaaaagt tgaagtaact
    24421 agtcaacgtt tagccaatgc gaaaaaatat tatggagaaa attccaccga agttcagaaa
    24481 cttgagagag aactgattaa tcaacaaaca gcgcaacaaa ggttatctaa tgatatcgat
    24541 aaaacgagta aagcattggc tcaatctaaa ggtgaattta aaacctatga gtcaactatg
    24601 aaggatttag ataatgagca acaacaccta aaatcaagtg catcattagt agaatccgaa
    24661 tacaaaaaat ggcaagcaac tgctggtcag tctgctaccg aatctgaaaa attagcaaaa
    24721 gcacaagaat atgtcggcaa acagagtgat attgctgaac aaaaaatcga agtgctaaaa
    24781 aaacaattag atgccacaca aaaagaattt ggagaaacat ctaccgaagc tttagatatg
    24841 aaaactaagc ttaatgatgc ggaaagagaa ttcgaagaat taagtaatgc tgctaaaaac
    24901 gttgatacat ccacagtaga cgatatcggc aaaaaacttg atatgggcaa tttaatggaa
    24961 gctactgatc acttgtcagt gatcggagat aagctgattg atgtaggcag taagtctatt
    25021 gaagcagctg gaaaagcaca agctatgcag gcccaattta aacaagtctt tggctcttta
    25081 gaaggggaag cgcaggacgc cgttgaggga atggctgaaa aatttggaat gttaccaaat
    25141 acgatcaagc ctgtttttac gcaatatacg tcaatgttta aaggacttgg atatgataca
    25201 aaagaagcta tggagttagc tggtgacagt actcagttag cagcagatgc agcagctttt
    25261 tacgataagt ctatggatga tgctagtgaa tctcttaatt cattcataaa agggaactac
    25321 gaaggtggag aacaaatagg tctatttgct aatgatactc aaatggctgc ttatgctgtt
    25381 aagcataact tgataccagc gactgaagga gcaaaaaaag ccagtgaaga gtcattgtta
    25441 gccgttgaaa aagcacaatc taagtatgct gatgctatta agaaacatgg cgaaggatct
    25501 ttagaagcaa gagaggctgc tttaaaactt aaagatgcgc aagataaaat aaatgaagaa
    25561 ttaggcccac aaactcaaaa atggtctgat ttagatgagg ctaccaaaca agcagttcga
    25621 gttcaatatg ctgaagatat gcaaaaatta gccggtgcta caggtcaggc tagtagagag
    25681 tctgatggtt tagaaaacca aatgacaaga gcaaagcaag cattagaaga tttttatgcc
    25741 tcgttgggtg aagatatatt gcctgtgttt atcaaagggt tacaagcagg cgcgaaagct
    25801 ttgcaaggat tagccgaatg gtggagtaaa cttgatggac caatgaaaaa tttcatttta
    25861 gctcttggag gaattctagc attattaagc acattagctc ctgttataac cgcagttgtt
    25921 acgatagttg gcacatttgg ttctacagtt ttgctgccaa taataggaat tatagcgggc
    25981 gttgcagctg taatagcgat tgtaatcaca gcgtttcaga actggggtgc aattactgac
    26041 tggtttaagg gaatatggag taaattcact gattggttgg gtggtacttg ggaaagcata
    26101 aaagaaggtg cctcatcagt ttgggatgga gttaaagaaa cctggtctgg atttgtagat
    26161 tgggttcaag aaatttggca aggagtttct gattggtttg gagagttatg gagcggatta
    26221 gttgaaggag cttccaacat ctggcaagga gtccaagaga cttggcaagc attcgttgat
    26281 tgggtttcaa atatttggaa cggagtcaaa gaagtatggt cgattatttg ggcagacatt
    26341 gtaggaattg ttcaaatacc atggacatta ataacgtcat tgattcaagc tggtattaat
    26401 attatcgtgg gtatctttga tgtagctgga cagttattag gcgcagcttg gcaagctgtt
    26461 tggacaccta tttctgattt ccttaaaaat acttgggata ctatgacaca atggataagc
    26521 atcgcttgga atggaattgt aactacattc catactatat ttgatccagt agtggcatgg
    26581 tggaatggta tatggacatc tattagtact acggcttcaa atatttggaa ctcaattagt
    26641 gcaacagctt ctagtatttg gaatagtatc aagaatacaa tcactagctt ggtacaagca
    26701 gctgctacag taattcaaaa tatttggtca actgtatcta gttggttagg tggaatttgg
    26761 aattcaatca gctctacagc atcaaatatc tggagtagcg tgactagtag tataagcaat
    26821 gctataaacg cagctaaaag tgccattcaa agtgtttgga atagtatatc ttcgtggatc
    26881 agcggaattt ggaacggtat caaaaacact gctttgaatc tttggaatgg aattacaagc
    26941 actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg gtccaatcta
    27001 acaggtattg tttccgacat attcaataat gttaaaagta cgattgctaa catttgggaa
    27061 ggtattaaaa agactgttag cgctccgatt gattggatta gagataaaat cagtggcatc
    27121 tttgataatc tgaatatttc gataccacat attccgttac cacattttaa attgagtggg
    27181 aaattcaatc cattgaaggg aaaaatccca acgttgggtg ttgattggta tgcgaaaggt
    27241 agtgtgttta attctccgaa tattatcggt atcggtgaag caggacctga agtagcttta
    27301 cctttgaaaa gatctgtgct gcaagaaatt ggggatcgta tcttgagtag cacctcagtt
    27361 tcatctaggg cacaaacggt tcaacctgtg aacaattacg aattcaattt cacaattgat
    27421 ggtaacgcag atgaggttac tatgaagcag acaactcaac aaatcattga cagcattaca
    27481 aaagttcaaa atgataatgc ttcggcatgg cgttaaacag gagagtattt ctcctgtttt
    27541 cttagtatta caaaggatgt gaaaaaatga ctgattgtat acattctata gtcgatggat
    27601 ttcctgatta tttgcataaa ttggctttag ctgaaagacc aaccatacct tctccaaaaa
    27661 gacagagagt tgaaacttct gttttaggac ggctaggtgg cttagtacaa gattattcgt
    27721 ttgaagatat gtcgtttact ttgcactata actatttaga ggatgtggaa gaccatcaag
    27781 cgttcaagca atcgttttat atcatgcgtc attggttaaa ttatgcaaag aaattagaat
    27841 tctctgatga tcccaacgtc tattatgtta tccagactat cgatattggg gatgcagaaa
    27901 acgatattgt tgaatgggga gagttcgatg taaatatcac tgcgaaacca ttcgcaagag
    27961 ttcaagaaga tgtacctata accgtagata aaccacagtc atttaacttg ctgaataata
    28021 gtttagaaga aagttttcca aagattatca tcactccttc agctacttca tgccaattca
    28081 tcttaaatga ttatgtgttt agttttgaag gcttagtagt aggaactgac atagtcattg
    28141 atagtgatct aatgctttgc tacgaagagc aatcggacgg agatatttta gatcggtcca
    28201 acaaaatgaa gaccatgcaa tatccgacat tgcaagtgga tatcaatcat tttaattgca
    28261 ctggtttgag caaaatacaa atttatcgta atgggttaag gtaggtgaaa tagatgatcg
    28321 ataatttaat aactatttac gataaaaatg acgcgaataa tttagctgaa catttatatg
    28381 atacgcaagg tttaggcgct ttgtcagact ggttaacagc cactgttagc aataaactaa
    28441 acggagccga gatatttcag ggtacttatc caataagcgg aactaatgca gatttgatta
    28501 tagaaggacg tattattcag tgctatgtag atgaaaatcg agcaaagcaa cgtttacgga
    28561 tttattatgc aaagacttct gtaataggaa atacgataga agtaaaagct gaacctattt
    28621 tcaatgatat aagaaaatcg gtgttgaata aatatgacag tggaacagaa aagatcactg
    28681 ctactcaggc atggcaaaac gcaaaagctt tagcgaaacc agctatccct tcgcagtttt
    28741 ctttctcgtc attagtagat acgcttgcta atgtgaagat agaaaaagcg aattttttag
    28801 aattctttgg tggaaaagag ggatctattc tagatcgatt tcatggggaa tttcttaaag
    28861 ataataacac attacgtcat gaaaaaaggc taggtacgga tcataaaatc aaagcgattt
    28921 atactaaaaa tttaactggt cttgacttag agatagatgc tcaaagcgtt ttagttggag
    28981 tttatccatt cattagcagc tcttcagaag gagaagacga gatcactcta ccagaagaag
    29041 ttattttcac ggattacgtg gatgattatc ctgctggata tgtttctttt gttgatttta
    29101 aagacaaagc gactgatgta gccacattaa gggaagctgc taaagactgg ttgaaaacaa
    29161 acatagataa acaaaaacca caagtgagtg gttcgattga attagtacca ttgaggcatc
    29221 aaagaggcta tgaaaaattt gttgatctag aaaaagtttc gatgggtgac ggagtagatg
    29281 tgtatcatcc acagttaaaa gtgaatatgt cagcgagaat tgtggaatat acgtttaatg
    29341 ttctaaccaa ttcatacgat aaattagttg taggaaacgt caaaacaaac ttcttagaaa
    29401 acacagagaa taatgtcagc aatttgatta atgatgccat tgatcaattg aaaaacggtg
    29461 gcgaaatcag tgatttaatc aatgatattg tagatcatca aactgatatg attactggtc
    29521 aaaatggtgg ttatgtttta ttagatccta aagaagcgcc tagtcgtatt ttgattatgg
    29581 acacaccaga taagaatacc gcaaggaatg ttttacaaat caacaatgct ggtattggtt
    29641 tttctaaaac tggcattaat ggaacatatg aaacggcatg gacgttagat ggcggattca
    29701 atgcctcgtt tattacggct ggtgaaatag taggaattac tattagaggt actacattaa
    29761 ttagtgatgg tgctgattat agaacaagta ttgctaatgg caaaatgact tggtactcaa
    29821 aaaaagttaa caaagatatt atggagctag aagcacgtga ttatgtaagt gctgatgccg
    29881 gtattgtatc atacaccatg aaaactggtg gtggtttcat gattagaaat ccacagggta
    29941 acttggtttt tagtacgtgg gataatggta ataacagacc gtttctatct tttggtgcgc
    30001 ccaatttcag gtatagcaat gctagttatg taacttctgg cgacggtagt tctttaagca
    30061 ttaatggtag tgcgggtaac tcatgggaat ttaaggtagc tggtaggact atgaaattta
    30121 ctagtgatgg tatgctaacg ttaccaggtt gtttttttgg ttcatgggaa gatgggaaac
    30181 ttgctaggtt tgaacaatca acggtacaag tatataaaga ttttactgtt agaggtacta
    30241 aaaactcaac tgtaccaaca gaacattatg gacaacgact attgaacgct tatgaaactc
    30301 cagaatatta tttcgctgat tatggggaag ccgttacagg tgacgatggt aaagttcgtg
    30361 ttgatattga ccccatgttt gctgagacag taaatctaag tcggtatatg acacatgtga
    30421 cacctacaga actagttttg tgtgctgtta ctcatgaaga tattgaccat ttcatcattg
    30481 aaactagtaa gccaaacgta ttagttagat ggattttagt ggcacaccgt ctagggtatg
    30541 aagatattag attaaaagag gatacagcat atgatagcac agtgcttgac caaaaacgtt
    30601 tttaaaacga agacaaggag gtatataaat ggctagcagt ttatataatt tggctttaga
    30661 tttcagcaaa gaattaaact acaccaaagc tattatggct cgtcagggtg ataaagggat
    30721 tacggtgacg gttaaaccat atttaaatgg cttgcagatg gatacgagtg gcggaacatt
    30781 tactttaaaa ggaacaacac catctaaccg ttacgtagat agtgttgcaa ctagcgtaac
    30841 tagtgaagaa gtcacgtttt ctcttgatgg cacatttatg agtgaagcag gatattataa
    30901 acactgctac gtagaatata gaaaagacaa tcaaatttta acaacgcaag atatcatttt
    30961 tttctcacta ggagtgtctg acatttcgca aggtcaagcc gatgaatatg tttcgcaatt
    31021 agaagagttg attcgaaagt ataatgaaac ttttgatgct tttatggctg aaactaaagg
    31081 tagagtggat agcttaaatc aacagattac tgatttaact ggtcaagcta aaacgctaca
    31141 agacaagtta gatgctctga aagaagaaat ttctaagtta ggtaacttac aagtgatgta
    31201 cagtaacagc attgatttcg ggggctatga ttattcgggg aatccgaatt tgatggctaa
    31261 tataaacgct gatagcttct cgcaaggtag tggtgcctta tctgttgtag atgatggtga
    31321 cgaggtagtg attacgcttg atccaaacca taaattagag gtattaaaac cgaaaagtca
    31381 accagcccta ttaacaggca aaacatatac cgtgagtgta gaaattatgt tagaaggtga
    31441 tttcactgga gaccctagca agataggcct aagatatatt aaaatgccta actgggtatc
    31501 agagctatat acgcgtaata cattaactgc tactaagggt gtatggcaaa aactaactgg
    31561 caccgttaaa attactgctg cgagtgataa cgctgaaagc tggcttataa tgctacaaaa
    31621 taaagacgct aataacagcc tatccggcaa actacgtttg agacacgcta aactcgaaga
    31681 aggctcaaca gctacaccat tccaacctaa cttattagca gaaccttaca acatgtgtcg
    31741 cgaatatcct aacgaaaata ttgccgatcc tacagttaag ttcccaatcg aatctggcga
    31801 ccaccaaata tatcaaggtt acacagaaga agagcttatg ataggtcaaa cgtatactat
    31861 cacgcttaaa ggaacaaaac ccgcaagtca aacctttgta gcgtataatc attggactgc
    31921 tcgtttagga gaactaaagc cggttgatgg gttgacagac gtatggtctc taacattcac
    31981 accaacgaat gttgtggcga tgcctaaact tttccgtgtt tatcagtatc cacgatcaac
    32041 agtaggcgca tgccggatcg actggctcaa gatcgaaaaa ggcaacactc gaaccccgaa
    32101 tattagtgag tataaatatc gtggtactgg tatgcgtgat tcaaacaatc caaaagatta
    32161 tgtttgggat ctagcaccag aatatgtcga agataatttg gccacagata ttaaaatttc
    32221 tgaaattact ggtaaagcaa acaattatac cgatgggaaa gtatcgaaga ttaattcgca
    32281 gttgactgct tcaattaatg aagtagacac cacagctaag ggtgctcaaa caaaagcgaa
    32341 tgctaatgcg actgctatag atgaattaga caataagatc gatgaacgca ttaatgatac
    32401 agctactacc gcattttatg gcgcacaagg tgaagtctct aacaaaacga aagtagcgga
    32461 gtatggagta ggaacagggt atactactac agctgtagga gaaacacatt tcgaacgtca
    32521 atctaacgga tacgttaaat gcttaaaagc tggtaagtat tcaattagtg cgcaaatacg
    32581 tgtgcaattg ggcgggaaat atgcttcatg gttatatact gatttatata aagatggaac
    32641 ttctgttgat aatttagtat cttatggggt cgacgccttg caaaatagat ttgcagctag
    32701 cggaaacatt gtaactgatt tagaagtaaa cgatgtacta aatatgcgta ctgaaatcgg
    32761 cgttgcaaat ggaattttac aatttacatc ttgccgtact ttagtcttga ctagactatc
    32821 tgattaatac aatatttata attttgctat caacacgctc aaaggagggt gttttttatt
    32881 ttgcaatgaa aggaggatag ttggttgaaa gacgaagcaa tacaagacgt ggtagaacgc
    32941 ttagtgcgta ttgaaacgaa actggataat tacgaattat tacgcgaaaa agcggaaagt
    33001 gcaaaagata gagcggatca ggcatattct attgcgctta ataatgcgga agatatcaaa
    33061 gaaatgaaag ccaataataa atggtcgtgg ggttacatga tcggtttagg cattacgatc
    33121 attggctatt tcttgactaa attgtaaagg aggtgagaag aaatgatttt acccgataag
    33181 tattatcaag tcattaaatg gacggtttta acagtattac cagctgcatc tgctttagta
    33241 gccatgttag gcaaagccta tggatggaat ggaacagata tgacagttct gactatcaat
    33301 gcagtagcca cgtttttagg cgttatcact ggtgtgtcgg cttataattt gaaaaaatag
    33361 gaggaaacaa atgaaaaaga aaattactat tactgcgatg agcctattaa cggctctttt
    33421 tttattgcca attaatgggt tcgcctatac gattaacaat gaatttaatt tgggcctaaa
    33481 cgaaggtagc tcacaagtag caaataatca gtacatttta ctgcatgaaa cggctaatga
    33541 aacagcaaca ggacgcaatg aagcgcaata tatgcaacgt tcatggacta gtgcttatac
    33601 tgcttatatt gtgggagacg gtggaattgt ttaccaagtt ggacaacctg gttatgtaca
    33661 gtacggtgct ggttcgtatg ctaatgctaa cagtcctgtg cagattgagt tacaacacac
    33721 acataataaa gcaacttttg aaaagaacta taaagcatac gttgaattgg cgagagattc
    33781 cgctaagaaa tacggtattc cgcttacatt ggacactcct tataaccaac caggaatcaa
    33841 atcacattta tgggtaacac aaaatatttg gggcgatcat acagatcctt acggttatct
    33901 ttctgaaatg ggcgtaagta aagaaaaatt agcatatgat ttagctcatg gatttaccga
    33961 tgaaaatcca acaacttctg aaaacaagcc tgtcattgat ccaacacgag ctggtgcagc
    34021 taatcctacg ctgacagatg gaacaaatca cgcccacatt gatcagtttg gggaaatcga
    34081 aaacgcaaac ttgcatgtgg ctggatggca cattgctaac tataaatacg agtatatttt
    34141 cattatggac tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag
    34201 accagatgta aaccaagctt ataatacttc tggaaatgtt ggttatcatg tatctttcaa
    34261 tatgcgtaat tttcctagta agaaagtcta tgtaatgatg cgggcaacga atgatccaga
    34321 gggaaacact aaaggcggtg cgcaagattt ccatgacaaa cgttggtatt taaatattcc
    34381 taaacgataa aaatagctcc tcgttgagga gcagtacata actatattga caactataaa
    34441 aattattcga taaaatagtg atgttatcgc atatcttcac tatcacccat aaatagtcac
    34501 actccaagct atgcgataac aggtttgttg ccacacattc tactggttga ttgtttatgg
    34561 ctttatgtgg caacaaccag tacccttagc tcagttggtc agagcagacg gctcataacc
    34621 gtccggtcgt aggttcgagt cctacagggt acattaacgt agccatttga atcgttctgt
    34681 gttagaattt ttttgaagag tattatacaa gctaaagctt ttcttcattg ccactcaaat
    34741 gagtggcttt tttatgtatc cttttatgga ttaatgaaag gatgtttcac atagttatac
    34801 ttctgtatat ttgaaaagtt ttactttgat ttttaaatag aaagacattt gggttaaatt
    34861 gtgagataat aataaagaag agtttaaagc gcaccccaaa ccacttcccc ataagtgtgt
    34921 tacgctttaa actcttttat atttgaagcc attaaaaagc ataccatatt tttgaaaaaa
    34981 agtgagaaaa aaggcttaca attggagtgg tagttaatta gtgacttatt tttgatttta
    35041 tagcactgat actataaaat atagatatca tcatattaca caatcttaat actaacttaa
    35101 aaaatatctc ctttcacaag tatggtgata aaattcgttc cgggctacct ttttaggtag
    35161 cctactttaa tctttatacc tttctggatc aacgaaagt
//
LOCUS       DACCQI010000010        34660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCQI010000010 DACCQI010000000
VERSION     DACCQI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.010"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(313..2205)
                     /locus_tag="IVO76_000421"
     CDS             complement(313..2205)
                     /locus_tag="IVO76_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428412.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="IVO76_000422"
     CDS             complement(2202..3929)
                     /locus_tag="IVO76_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428413.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="IVO76_000423"
     CDS             complement(3945..4595)
                     /locus_tag="IVO76_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7428414.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="IVO76_000424"
     CDS             4799..5095
                     /locus_tag="IVO76_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7428415.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="IVO76_000425"
     CDS             5287..5907
                     /locus_tag="IVO76_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7428416.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="IVO76_000426"
     CDS             complement(5999..7165)
                     /locus_tag="IVO76_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7428417.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="IVO76_000427"
     CDS             complement(7177..7530)
                     /locus_tag="IVO76_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7428418.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="IVO76_000428"
     CDS             7658..8680
                     /locus_tag="IVO76_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7428419.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9119..9553)
                     /locus_tag="IVO76_000429"
     CDS             complement(9119..9553)
                     /locus_tag="IVO76_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428420.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="IVO76_000430"
     CDS             complement(9663..11522)
                     /locus_tag="IVO76_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7428421.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12799)
                     /locus_tag="IVO76_000431"
                     /pseudo
     CDS             complement(11640..12799)
                     /locus_tag="IVO76_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13106..14530)
                     /gene="araA"
                     /locus_tag="IVO76_000432"
     CDS             complement(13106..14530)
                     /gene="araA"
                     /locus_tag="IVO76_000432"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP7428422.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14546..15244)
                     /locus_tag="IVO76_000433"
     CDS             complement(14546..15244)
                     /locus_tag="IVO76_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7428423.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15201..16862)
                     /locus_tag="IVO76_000434"
     CDS             complement(15201..16862)
                     /locus_tag="IVO76_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP7428424.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17080..18480
                     /locus_tag="IVO76_000435"
     CDS             17080..18480
                     /locus_tag="IVO76_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP7428425.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18732..19811)
                     /locus_tag="IVO76_000436"
     CDS             complement(18732..19811)
                     /locus_tag="IVO76_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7428426.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19897..20856)
                     /locus_tag="IVO76_000437"
     CDS             complement(19897..20856)
                     /locus_tag="IVO76_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7428427.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20868..22391)
                     /locus_tag="IVO76_000438"
     CDS             complement(20868..22391)
                     /locus_tag="IVO76_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7428428.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22401..22964)
                     /locus_tag="IVO76_000439"
     CDS             complement(22401..22964)
                     /locus_tag="IVO76_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7428429.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22970..23833)
                     /locus_tag="IVO76_000440"
     CDS             complement(22970..23833)
                     /locus_tag="IVO76_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7428430.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23847..24743)
                     /locus_tag="IVO76_000441"
     CDS             complement(23847..24743)
                     /locus_tag="IVO76_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7428431.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24918..26189)
                     /locus_tag="IVO76_000442"
     CDS             complement(24918..26189)
                     /locus_tag="IVO76_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7428432.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26179..27696)
                     /locus_tag="IVO76_000443"
     CDS             complement(26179..27696)
                     /locus_tag="IVO76_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7428433.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27894..28784
                     /locus_tag="IVO76_000444"
     CDS             27894..28784
                     /locus_tag="IVO76_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7428434.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="IVO76_000445"
     CDS             complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="IVO76_000445"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7428435.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29693..31165)
                     /locus_tag="IVO76_000446"
     CDS             complement(29693..31165)
                     /locus_tag="IVO76_000446"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7428436.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31802..32164)
                     /locus_tag="IVO76_000447"
     CDS             complement(31802..32164)
                     /locus_tag="IVO76_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7428437.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32387..33139
                     /locus_tag="IVO76_000448"
     CDS             32387..33139
                     /locus_tag="IVO76_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7428438.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33279..34202)
                     /locus_tag="IVO76_000449"
     CDS             complement(33279..34202)
                     /locus_tag="IVO76_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7428439.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34217..34489)
                     /locus_tag="IVO76_000450"
     CDS             complement(34217..34489)
                     /locus_tag="IVO76_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428440.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10230 a         7212 c         5979 g        11239 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    12061 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    12121 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    12181 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12241 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12301 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12361 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12421 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12481 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12541 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12601 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12661 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12721 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12781 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12841 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12901 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12961 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    13021 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    13081 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    13141 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    13201 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13261 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13321 tcagcagttt ataatgcgta ccgaaatctg ccatcgatac gactacccct tctcctgctt
    13381 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13441 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13501 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13561 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13621 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13681 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13741 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13801 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13861 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13921 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13981 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    14041 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    14101 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    14161 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    14221 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14281 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14341 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14401 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14461 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14521 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14581 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14641 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14701 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14761 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14821 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14881 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14941 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    15001 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    15061 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    15121 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    15181 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15241 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15301 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15361 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15421 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15481 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15541 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15601 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15661 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15721 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15781 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15841 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15901 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15961 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    16021 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    16081 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    16141 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    16201 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16261 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16321 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16381 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16441 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16501 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16561 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16621 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16681 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16741 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16801 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16861 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16921 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16981 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    17041 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    17101 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    17161 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    17221 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17281 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17341 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17401 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17461 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17521 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17581 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17641 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17701 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17761 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17821 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17881 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17941 gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg
    18001 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    18061 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    18121 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    18181 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18241 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18301 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18361 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18421 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18481 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt
    18541 cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc
    18601 agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg
    18661 atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa
    18721 aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca
    18781 tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca
    18841 tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg
    18901 cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt
    18961 tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct
    19021 aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc
    19081 gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg
    19141 ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga
    19201 cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct
    19261 aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg
    19321 ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta
    19381 ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc
    19441 aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca
    19501 ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca
    19561 ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat
    19621 gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc
    19681 cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt
    19741 gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga
    19801 tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc
    19861 aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc
    19921 accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt
    19981 aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaaat
    20041 atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt
    20101 tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag
    20161 attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc
    20221 agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt catttactga
    20281 aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa
    20341 cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc
    20401 ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact
    20461 gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc
    20521 tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt
    20581 atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc
    20641 ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc
    20701 atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac
    20761 cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg
    20821 ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt
    20881 attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct
    20941 attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc
    21001 aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt
    21061 tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg
    21121 ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa
    21181 acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc
    21241 atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt
    21301 attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata
    21361 gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg
    21421 ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca
    21481 tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta
    21541 gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta
    21601 tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag
    21661 ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat
    21721 tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca
    21781 tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc
    21841 ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca
    21901 aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa
    21961 ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg
    22021 aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa
    22081 tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct
    22141 acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct
    22201 aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt
    22261 tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag
    22321 ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact
    22381 tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt
    22441 ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca
    22501 aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat
    22561 gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc
    22621 caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg
    22681 attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta
    22741 aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt
    22801 cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg
    22861 atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa
    22921 accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca
    22981 ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta
    23041 atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc
    23101 ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca
    23161 atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag
    23221 taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa
    23281 tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga
    23341 taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa
    23401 gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca
    23461 gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc
    23521 ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat
    23581 ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat
    23641 agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag
    23701 aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg
    23761 ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc
    23821 tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac
    23881 acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat
    23941 gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt
    24001 tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc
    24061 gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta
    24121 atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag
    24181 ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa
    24241 acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc
    24301 ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata
    24361 gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga
    24421 caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata
    24481 atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct
    24541 cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag
    24601 aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat
    24661 aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc
    24721 caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca
    24781 atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa
    24841 cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc
    24901 ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc
    24961 atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg
    25021 ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt
    25081 ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc
    25141 aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa
    25201 tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca
    25261 tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta
    25321 gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt
    25381 tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct
    25441 ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca
    25501 ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac
    25561 atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta
    25621 ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt
    25681 tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt
    25741 acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct
    25801 ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct
    25861 ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt
    25921 ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt
    25981 tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt
    26041 tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca
    26101 gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa
    26161 gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca
    26221 tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt
    26281 tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt
    26341 atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg
    26401 aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc
    26461 atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt
    26521 tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa
    26581 tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat
    26641 aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt
    26701 gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg
    26761 aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt
    26821 ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc
    26881 gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg
    26941 agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc
    27001 gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga
    27061 acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc
    27121 ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat
    27181 ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct
    27241 taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc
    27301 atctactcca cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca
    27361 ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc
    27421 caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac
    27481 aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac
    27541 gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata
    27601 tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac
    27661 acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt aatttataca
    27721 tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt
    27781 ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt taatataact
    27841 tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac
    27901 tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca
    27961 agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg
    28021 gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca
    28081 aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca
    28141 gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc
    28201 actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg
    28261 ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct
    28321 ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat
    28381 tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt
    28441 ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg
    28501 gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc
    28561 aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac
    28621 ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa
    28681 tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg
    28741 gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat
    28801 tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat
    28861 ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac
    28921 catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca
    28981 atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca
    29041 agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat
    29101 tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca
    29161 aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct
    29221 gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct
    29281 ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca
    29341 gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt
    29401 gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt
    29461 tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa
    29521 gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc
    29581 aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt
    29641 ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt
    29701 ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc
    29761 agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt
    29821 cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt
    29881 cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc
    29941 gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc
    30001 gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag
    30061 tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact
    30121 tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac
    30181 caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt
    30241 tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga
    30301 tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg
    30361 ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg
    30421 ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca
    30481 cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt
    30541 ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta
    30601 cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat
    30661 ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt
    30721 tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg
    30781 tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa
    30841 aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat
    30901 actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat
    30961 tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc
    31021 cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac
    31081 ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac
    31141 tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct
    31201 acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca
    31261 catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt
    31321 attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga
    31381 agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat
    31441 cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta caacataatc
    31501 aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag
    31561 aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat
    31621 accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta
    31681 agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt
    31741 gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt
    31801 attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt
    31861 tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt
    31921 ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt
    31981 tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg
    32041 ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca
    32101 ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct
    32161 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
    32221 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
    32281 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
    32341 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
    32401 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
    32461 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
    32521 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
    32581 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
    32641 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
    32701 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
    32761 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
    32821 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
    32881 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
    32941 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
    33001 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
    33061 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
    33121 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
    33181 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
    33241 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
    33301 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
    33361 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
    33421 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
    33481 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
    33541 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
    33601 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
    33661 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
    33721 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
    33781 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
    33841 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
    33901 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
    33961 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
    34021 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
    34081 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
    34141 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
    34201 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
    34261 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
    34321 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
    34381 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
    34441 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
    34501 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
    34561 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
    34621 ttataatgat actgtaaata tatctttatt attagtgaat
//
LOCUS       DACCQI010000011        33817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCQI010000011 DACCQI010000000
VERSION     DACCQI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.011"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            299..1960
                     /locus_tag="IVO76_000451"
     CDS             299..1960
                     /locus_tag="IVO76_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7428441.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IVO76_000452"
     CDS             complement(2030..2377)
                     /locus_tag="IVO76_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7428442.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IVO76_000453"
     CDS             2461..3402
                     /locus_tag="IVO76_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428443.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IVO76_000454"
     CDS             3403..4446
                     /locus_tag="IVO76_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428444.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IVO76_000455"
     CDS             4452..5522
                     /locus_tag="IVO76_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428445.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IVO76_000456"
     CDS             5512..6465
                     /locus_tag="IVO76_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7428446.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IVO76_000457"
     CDS             6477..7487
                     /locus_tag="IVO76_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7428447.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IVO76_000458"
     CDS             complement(7772..8593)
                     /locus_tag="IVO76_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7428448.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IVO76_000459"
     CDS             complement(8690..8968)
                     /locus_tag="IVO76_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428449.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IVO76_000460"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IVO76_000460"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7428450.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IVO76_000461"
     CDS             complement(11724..12311)
                     /locus_tag="IVO76_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428451.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IVO76_000462"
     CDS             12486..13808
                     /locus_tag="IVO76_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7428452.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IVO76_000463"
     CDS             13963..14739
                     /locus_tag="IVO76_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7428453.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IVO76_000464"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IVO76_000464"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7428454.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IVO76_000465"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IVO76_000465"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7428455.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IVO76_000466"
     CDS             16629..17258
                     /locus_tag="IVO76_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428456.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IVO76_000467"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IVO76_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7428457.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IVO76_000468"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IVO76_000468"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7428458.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IVO76_000469"
     CDS             19445..19981
                     /locus_tag="IVO76_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7428459.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IVO76_000470"
     CDS             complement(20021..21784)
                     /locus_tag="IVO76_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428460.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IVO76_000471"
     CDS             complement(21787..23502)
                     /locus_tag="IVO76_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428461.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IVO76_000472"
     CDS             complement(24281..24505)
                     /locus_tag="IVO76_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428462.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24705..27473
                     /locus_tag="IVO76_000473"
     CDS             24705..27473
                     /locus_tag="IVO76_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7428463.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27554..27973
                     /locus_tag="IVO76_000474"
     CDS             27554..27973
                     /locus_tag="IVO76_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7428464.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="IVO76_000475"
     CDS             27985..28455
                     /locus_tag="IVO76_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7428465.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="IVO76_000476"
     CDS             28472..29251
                     /locus_tag="IVO76_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7428466.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="IVO76_000477"
     CDS             29238..30059
                     /locus_tag="IVO76_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7428467.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="IVO76_000478"
     CDS             30076..31125
                     /locus_tag="IVO76_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7428468.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="IVO76_000479"
     CDS             31138..32142
                     /locus_tag="IVO76_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7428469.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="IVO76_000480"
     CDS             32775..33794
                     /locus_tag="IVO76_000480"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7428470.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11042 a         5991 c         6908 g         9876 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DACCQI010000012        31687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCQI010000012 DACCQI010000000
VERSION     DACCQI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.012"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(76..543)
                     /locus_tag="IVO76_000481"
     CDS             complement(76..543)
                     /locus_tag="IVO76_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7428471.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="IVO76_000482"
     CDS             complement(663..2201)
                     /locus_tag="IVO76_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7428472.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="IVO76_000483"
     CDS             2281..2967
                     /locus_tag="IVO76_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7428473.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IVO76_000484"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IVO76_000484"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7428474.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IVO76_000485"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IVO76_000485"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7428475.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="IVO76_000486"
     CDS             complement(4453..5583)
                     /locus_tag="IVO76_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7428476.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="IVO76_000487"
     CDS             complement(5583..7766)
                     /locus_tag="IVO76_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7428477.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="IVO76_000488"
     CDS             complement(7763..8788)
                     /locus_tag="IVO76_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7428478.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="IVO76_000489"
     CDS             complement(8833..9660)
                     /locus_tag="IVO76_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7428479.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="IVO76_000490"
     CDS             complement(9657..10724)
                     /locus_tag="IVO76_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7428480.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="IVO76_000491"
     CDS             complement(10750..11586)
                     /locus_tag="IVO76_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7428481.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="IVO76_000492"
     CDS             complement(11583..12452)
                     /locus_tag="IVO76_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7428482.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="IVO76_000493"
     CDS             complement(12522..13805)
                     /locus_tag="IVO76_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7428483.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="IVO76_000494"
     CDS             complement(13819..15504)
                     /locus_tag="IVO76_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7428484.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="IVO76_000495"
     CDS             15779..16804
                     /locus_tag="IVO76_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7428485.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IVO76_000496"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IVO76_000496"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7428486.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IVO76_000497"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IVO76_000497"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7428487.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="IVO76_000498"
     CDS             25476..26444
                     /locus_tag="IVO76_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7428488.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="IVO76_000499"
     CDS             26462..27367
                     /locus_tag="IVO76_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428489.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="IVO76_000500"
     CDS             27364..28170
                     /locus_tag="IVO76_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7428490.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28327..29295
                     /locus_tag="IVO76_000501"
     CDS             28327..29295
                     /locus_tag="IVO76_000501"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7428491.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29612..30136)
                     /locus_tag="IVO76_000502"
     CDS             complement(29612..30136)
                     /locus_tag="IVO76_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7428492.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30423..>31687
                     /gene="scm"
                     /locus_tag="IVO76_000503"
     CDS             30423..>31687
                     /gene="scm"
                     /locus_tag="IVO76_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7428493.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTST"
BASE COUNT         8903 a         6856 c         5965 g         9963 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctctct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct attaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtgtttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatacaat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcgcgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    28201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    28261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    28321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    28381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    28441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    28501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    28561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    28621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    28681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    28741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    28801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    28861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    28921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    28981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    29041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    29101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    29161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    29221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    29281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    29341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    29401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    29461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    29521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    29581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    29641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    29701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    29761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    29821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    29881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    29941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    30001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    30061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    30121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    30181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    30241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    30301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    30361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    30421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    30481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    30541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    30601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    30661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    30721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    30781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    30841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    30901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    30961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    31021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    31081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    31141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    31201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    31261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    31321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    31381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    31441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca
    31501 gtacagatac tacctctacc acaagtgata gcacggatac atcagcttca agcgacagta
    31561 cagatactac ctctaccaca agtgatagca cggacacatc agcttcaagt gacagtacag
    31621 atactacctc taccacaagt gatagcacgg acacgtcagc ttcaagcgac agtacggata
    31681 catctac
//
LOCUS       DACCQI010000013        31671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCQI010000013 DACCQI010000000
VERSION     DACCQI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.013"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(172..618)
                     /locus_tag="IVO76_000504"
     CDS             complement(172..618)
                     /locus_tag="IVO76_000504"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7428494.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(629..2842)
                     /locus_tag="IVO76_000505"
     CDS             complement(629..2842)
                     /locus_tag="IVO76_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7428495.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(3075..3839)
                     /locus_tag="IVO76_000506"
     CDS             complement(3075..3839)
                     /locus_tag="IVO76_000506"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7428496.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(3840..4787)
                     /gene="prmA"
                     /locus_tag="IVO76_000507"
     CDS             complement(3840..4787)
                     /gene="prmA"
                     /locus_tag="IVO76_000507"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7428497.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(4800..5288)
                     /locus_tag="IVO76_000508"
     CDS             complement(4800..5288)
                     /locus_tag="IVO76_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7428498.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(5254..5925)
                     /locus_tag="IVO76_000509"
     CDS             complement(5254..5925)
                     /locus_tag="IVO76_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7428499.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            6063..7349
                     /locus_tag="IVO76_000510"
     CDS             6063..7349
                     /locus_tag="IVO76_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7428500.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7421..7615
                     /gene="ssrS"
                     /locus_tag="IVO76_000511"
     ncRNA           7421..7615
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVO76_000511"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7693..7977
                     /locus_tag="IVO76_000512"
     CDS             7693..7977
                     /locus_tag="IVO76_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428501.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            8050..8529
                     /locus_tag="IVO76_000513"
     CDS             8050..8529
                     /locus_tag="IVO76_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7428502.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            8752..8895
                     /locus_tag="IVO76_000514"
     CDS             8752..8895
                     /locus_tag="IVO76_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7428503.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            9473..11056
                     /locus_tag="IVO76_000515"
     CDS             9473..11056
                     /locus_tag="IVO76_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7428504.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(11493..12677)
                     /locus_tag="IVO76_000516"
     CDS             complement(11493..12677)
                     /locus_tag="IVO76_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7428505.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12702..13709)
                     /locus_tag="IVO76_000517"
     CDS             complement(12702..13709)
                     /locus_tag="IVO76_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7428506.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(13881..14240)
                     /locus_tag="IVO76_000518"
     CDS             complement(13881..14240)
                     /locus_tag="IVO76_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428507.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(14212..14583)
                     /locus_tag="IVO76_000519"
     CDS             complement(14212..14583)
                     /locus_tag="IVO76_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7428508.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(14600..14902)
                     /locus_tag="IVO76_000520"
     CDS             complement(14600..14902)
                     /locus_tag="IVO76_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428509.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(14919..15389)
                     /locus_tag="IVO76_000521"
     CDS             complement(14919..15389)
                     /locus_tag="IVO76_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7428510.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(15364..15642)
                     /locus_tag="IVO76_000522"
     CDS             complement(15364..15642)
                     /locus_tag="IVO76_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7428511.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(15639..16688)
                     /locus_tag="IVO76_000523"
     CDS             complement(15639..16688)
                     /locus_tag="IVO76_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP7428512.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(16645..17574)
                     /gene="cpaF"
                     /locus_tag="IVO76_000524"
     CDS             complement(16645..17574)
                     /gene="cpaF"
                     /locus_tag="IVO76_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7428513.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17706..19460
                     /gene="ade"
                     /locus_tag="IVO76_000525"
     CDS             17706..19460
                     /gene="ade"
                     /locus_tag="IVO76_000525"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7428514.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(19558..20220)
                     /gene="trmB"
                     /locus_tag="IVO76_000526"
     CDS             complement(19558..20220)
                     /gene="trmB"
                     /locus_tag="IVO76_000526"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7428515.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(20290..21069)
                     /locus_tag="IVO76_000527"
     CDS             complement(20290..21069)
                     /locus_tag="IVO76_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7428516.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(21276..22487)
                     /locus_tag="IVO76_000528"
     CDS             complement(21276..22487)
                     /locus_tag="IVO76_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428517.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(22480..23217)
                     /locus_tag="IVO76_000529"
     CDS             complement(22480..23217)
                     /locus_tag="IVO76_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428518.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            23355..23789
                     /locus_tag="IVO76_000530"
     CDS             23355..23789
                     /locus_tag="IVO76_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7428519.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            23782..24126
                     /locus_tag="IVO76_000531"
     CDS             23782..24126
                     /locus_tag="IVO76_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7428520.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            24306..25316
                     /locus_tag="IVO76_000532"
     CDS             24306..25316
                     /locus_tag="IVO76_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7428521.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(25385..26329)
                     /locus_tag="IVO76_000533"
     CDS             complement(25385..26329)
                     /locus_tag="IVO76_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7428522.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(26313..29033)
                     /locus_tag="IVO76_000534"
     CDS             complement(26313..29033)
                     /locus_tag="IVO76_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7428523.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(29030..30250)
                     /locus_tag="IVO76_000535"
     CDS             complement(29030..30250)
                     /locus_tag="IVO76_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7428524.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(30531..31376)
                     /locus_tag="IVO76_000536"
     CDS             complement(30531..31376)
                     /locus_tag="IVO76_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7428525.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         9445 a         6500 c         5490 g        10236 t
ORIGIN      
        1 tcttcagcaa cctcctataa aaaataactt gtataattta agcttaactt tttttccgac
       61 tgctgacaaa agaagcgctt tttgcagaca aaaaagccac ttaagttaga gtttatcagt
      121 ctaacttaag cgacgtgttt catagttttt actagtgaaa accagattac tttatttgtt
      181 tttagaatca ataatgatcg ttacaggtcc atcattgacg agagaaaccg acatatctgc
      241 tccgaattgt ccggtagcga ctgtgatgcc gcgacttctg attccttcat tgaatgattc
      301 gtataaagga atagctgttt ctggtcgtgc ggcttccaca aagcttggtc gatttccttt
      361 tttcgtatcc gcaaacagtg tgaattgaga gatacttaaa atttgtcctc ccacatcttc
      421 tacactaata ttcatttttc cttgttcatc ttcaaaaata cgcatttgtg caattttttt
      481 taccagatag tccacatctt tttgcgtatc ggtttcatga atgcctaata aaacaagtag
      541 gccgtagcca atttttccaa tttcttgctg atcgactgac acagcagctt tacttacccg
      601 ttgaacaact gctctcatat ctgtcctcct atccattcgt ccggcgtaca ctgtagacat
      661 caggtactgc tttgatttta tccacgatac tctttaggtg ggataaattc tggataccaa
      721 ccgtcaaatg gatcgtcgcc attttatctt tattcgtccg tgcttcaacg ctgttgagat
      781 ttttcgttag cgcattgact gtttgaagta catcattcaa taagcctgaa cgattatagc
      841 cgtaaatttc caaatctgca tcatactctt ttcgtgtatt cgatgtatct tcccattcga
      901 cttcaatcaa acgttctgct acatttggtt tgtcaggctg aacattcgga caatctcgac
      961 gatgaatgga tatccctctg cctttagtga tataaccaac gatatcatca ccaggaatcg
     1021 gattacagca gcgactgata cgaattagta agttttctac cccttgaatg acaacgccac
     1081 cttcatgacg tactttcatt ttgtcaggtt cttttttcgg ctgattcatg atttcttctg
     1141 cttcttgctt ttgctgctcg attttttgtt ctttacgttc tttttcagtc agacgattgg
     1201 ccatcgtcaa ggggctaact tctccatacc ctacagctgc atagagatca tcttctgttt
     1261 gataattaaa acgatcgagt gcttcttgca gtttgttctt cgtcaaaata tctttaggcg
     1321 taaatcctag atctgtaata catttgacca cggattcgtg gcctttgatc acattttctt
     1381 ctcgatcttg ggctttgaag aaacgtttga tcttatttct tgctttgcta gtagcaacta
     1441 atttcaacca gtcgcgactt gggccaaatg aatttggaga agtcatgatc tcaatgatat
     1501 ctccgttttt caatttgtaa tcaagctgca ccattttgcc atttactttt gcaccagtgg
     1561 ttttgttacc gatatctgta tggatactgt atgcaaagtc caatggtccg gatccttttg
     1621 gtaactcagt aacgtctcct tttggtgtga agacgtagac tttatcacta aagatatctc
     1681 cttttacgcc ttccataaat tcagaagcat catagctttc gtcttgaagt tcgagtatct
     1741 catggaacca gcttaattgt ttcgtcatac catctggttc tactttttca ttttttcctt
     1801 ctttataggc ccagtgtgca gcaaccccga attcagcgat ttcatgcatt tcctgtgttc
     1861 ggatttgaat ttctaccgga tttcctgccg gaccgatcac ggtagtatgc aaagattggt
     1921 acatattagc ttttggcatg gcaatataat ccttaaatct gccgggcatt ggtttccatt
     1981 ttgtgtggat tgctcctaat acagcataac aatccttgat tgaatctacg atcactcgaa
     2041 tagctagcaa gtcataaatt tcgttgaact gctttttctg atctttcatt ttacgataga
     2101 ttgaataaat gtgttttggc cgtccataga tttctgcaaa aatccccagc tcttctgtcg
     2161 ctattcgaat atcttcaaca gtaccactta cgtatttttc acgttcttct ctcttcgtct
     2221 gcatcaagtg gacgatacgg taatactgtt ttggatttag ataacgaagt gctgtatctt
     2281 ctaattccca tttgatccgg ctgatcccta gacgatgtgc aagaggcgca tagatttcta
     2341 aagtttcctg agcgatcctt ctttgcttat cttctcgcag gtgcttcaac gtacgcatgt
     2401 tatgtagtct atcagctaat ttgaccatga taactcgtaa atcttgagcc attgctagca
     2461 gcatctttcg gtggttttct gctagctgct cttcgtggga tttatatttt attttcccta
     2521 atttggttac gccgtccact aacatagcaa cgtcatcgcc aaattcttct ctcagatctt
     2581 ctaaagtgat ttctgtatct tcaacaacat catgtaagaa gcctgtagcc actgtatggg
     2641 gatccatttt taaatctgct aatatacctg cgacttggat aggatgaata atgtagggtt
     2701 cgcctgattt tctaaactgt ccgtcatgtg cagcagtcgc gtattcgcat gctttctcaa
     2761 caaatgctac atgttcggga cccatgtatt gactaacgag ctttatgaca ccgggtcccg
     2821 ttaaaatttc ttcttttgtc acaggaatcg atcccccttt cttttttcgg taaaacggga
     2881 atcgggacaa atatctgttt gataattgtc ccaaaatccg tgcagttata cgttttttta
     2941 cggaaatgtt cgctttttaa aagctttttc aaacgatttt tcgtatccat tgtataaata
     3001 attgtttcta ttatacaaat tgttgactat tttttcaatt ctttctgtcg atactctttt
     3061 attaatgaag aacgttagct gaaagaagga ttcattaatt cccattgata gctagcagca
     3121 cttaataaat aaagaggtgc tgtttctgta cgaaggattc ttggtcctaa tccacaaagt
     3181 atccctttgt gttctacaaa ataatcgact tcatcgcggg ataatccacc ttctggtcca
     3241 aagacagcta agattcgact atctggtttt gtttcactaa gaattttggc tgcatttgat
     3301 ttttcgcctt gctttgctga ttcctcataa gcaaccaaga ccacgtcata atcatctagc
     3361 cgctcaaata aatcttgttt cgtttccaat aactctactg atggcacgat ctgccgatgg
     3421 gattgttccg ctgcctcttg agcaatcttt tcaagccgtt gttgtcgttt catacgtttt
     3481 tttgcatccc atttaacgac agaggctgat gccgggaaac caagaaaagc ttgcgctcct
     3541 aattcagtgg atttctgaac gatccaatcc agcttttccc ctttaggata gccactcgct
     3601 atggtaatcg aacttggcag ttccttttga ttttcttctt ttttaatttc tttcagataa
     3661 acagcctctt cagtaatctc cgtgatttct gcaacaatgg ctatttgatc attgaatgcc
     3721 aaaaaaactt catcctcggg ttccatcctc ataacccgta caatgtggtg atagttttcc
     3781 ccttctaaaa caaaatagtc ttttgattca tatgattctt ttaaaaaata ccgctgcatc
     3841 tattccacct ctggtttttt caaaataatt gcataccagt ctttttgctg gaaaatttga
     3901 tccacgataa acccttgttc taacatggca tccagaacca tttgcttctt ctcttcaatg
     3961 attcctgaaa cgatcagcgt gcctgtttgc ttcagtaaac gccaagcatc tgggatcatc
     4021 aataagatga tatctgctaa aatgttcgca acgattacat cactttcaat ctcgattcct
     4081 gtcaaaagat cgttagctga tacatggaca tctttggcta tttcattcat atccatattt
     4141 tctttcgccg aacgaacggc tacttcgtct aaatcaaagg catagacatc ttttgcacca
     4201 agatattttg ctgcaatact tagcacgccc gacccagttc ccacatctag caatgtttca
     4261 ccaccacgca agactgtttc tagtgctgtc aatgtcaagg aagtcgtggg atgagtgcct
     4321 gtgccaaatg ccataccagg atctagtgtg ataatttttt ctagaggatc ttctgtctgg
     4381 tagttttccc agcttggcgc aaccgtcagc attcgcgtaa tacgcaccgg atgatagtat
     4441 ttcttccagg ctgttgccca gttgctttcc tctacttcac taactgttac tttatttttt
     4501 ccaatagcta gaccaaactc aggtaattgt gtgatacgat ctttgataaa aggcaagatt
     4561 tccggtaaga aagtcgtttc cggaaaataa gccatgacta gtgcgccttc tttcagcgat
     4621 gtaaaatcat ttttatctaa aatctcacca tattgatccg actgaaaatg ttcaacatct
     4681 aaagaatctt caatcgcgac accactcgcc cctgcctcca tcaaaatatt agcaactgct
     4741 tcgactgctt cactttcagt ttctactttt acttcattcc atttcatatc tgtcactcct
     4801 taataacttg ggtacggtaa ataagtatct gcttcatttt gctgttttac ttctactaat
     4861 tctgctagtt gctgattatc ccagtgaagc tcgaatacag cttcgtcatc gtcttcatcc
     4921 aaaaaatcaa gcaaatcgct ttgcccttga gctaatactt catttagata ttccactaaa
     4981 gaatcagcta ctgattttgc caaccctttt tttccttcat aagggattac tgctagataa
     5041 ttgcgttcat caaaaacaga tttctgagga ttaaaaagaa gtacgccgtc ttcaaattcg
     5101 ataattggtt cttgcgaaac gatcccttct gcatcatcaa tctcaaattg tgctttattt
     5161 tctgcaaaca agcgaacaac gatctcgatc gtatgatttt ttgtatccca atctaaagct
     5221 acatcatagt ctgtcacttg tttttcaatt atttcatcta aatatgttaa tagatccttc
     5281 tttttcattt tctttcctcc aaccccaatc tttctcgatt tggttttttc tttgtaaaga
     5341 gatataagga tttcccgaca caacaaaacg caacggcttt tctgtccata cacctttgtt
     5401 tgggataccg attctaggca aagcattgat cttttttggt gtcttttttt tctcaaaaac
     5461 taagcgtaac gaactatctg ctatagactg accataaagt tcttgaggaa ttgctaatgc
     5521 ttccactaat tttcccggac cgttgctgat atctggacca gtttttccgc cacgattttt
     5581 actcatttga tcaatcattg cggcgggttc gattgcacgt atcattactc cttgaggaat
     5641 cccttctggc tgagtaatga tgttcaaaat ccgatgagta tgcatcgtgt aaaggtagat
     5701 cgtacctggt ttttcataca ttgcccgcac acgcggtgtt cttcttaaac catagctatg
     5761 ggcggcctcg tcttctggac ctaggtatgc ttccgcgtct acaatatatc cagctaatct
     5821 tccttctggt gtgtcatgct ccaaatacat ccccaataaa tcccgtgcca cttctggcgt
     5881 cgttttattc caaaaaaatt catctatctt ttgtttatcc gtcatttttc tcacctactt
     5941 tatcatacac aatattagtt ggataagcta tgaaaacaaa acaacagaag gctgttcttt
     6001 ctaggcatct tgctaaatat gatataatta agggacgtaa aaacaagaag gaggaaaatc
     6061 ctatgcaaca gccattggct tatcgcatga gacctcgaac gatcgatgaa gtcgttggtc
     6121 agcagcatct agtcggggaa ggaaaaatca tacgccgcat ggtcgatgct cgcatgctct
     6181 cttccatgat tctatatgga cctcctggta ctggaaaaac gagtatcgca agtgcgatcg
     6241 ccggctctac taattatgca tttcgcatgc taaacgccgc tacagattca aaaaaggatc
     6301 tacaagttgt agcggaagaa gcaaaaatga gtggtacagt gatcctctta ttggatgaag
     6361 tacaccgatt agataaaaca aaacaggatt ttctgcttcc tcatttagaa agtggccgaa
     6421 tcatcttgat cggagcgact actgagaatc cttatattac catcaatcca gcgatccgca
     6481 gccgtacaca gatctttgaa gtcaaaccat taacagaaca agacatccag ttggcagtcg
     6541 aacatgcttt aagggataaa gaacgcggcc ttggacaaca agcaatccag ttagacgaag
     6601 atgctcttct tcacctttcg cgtgcaacga acggcgattt gcggagtgca ttgaatgggc
     6661 tggaactggc tacactatcg actccttcag ataaagaagg aaggattcat cttacactgt
     6721 cgatcattga agaatgtgtc caaagaaaag ctcttaccca tgacaaaaat ggagatgctc
     6781 attatgacgt tatttcggct tttcaaaaat cgattcgtgg aagtgatgtg gatgctgcac
     6841 ttcattatct cgcccgttta gttgaggcag gtgatctggc aagtatttgc cggcgtctga
     6901 tggttatcgg ttatgaagat ataggtttag gtaatcctgc tgctgcggct cgtacagtga
     6961 acgctgtatt agctgctgaa aaattgggat tgcctgaggc acgtatccca ctagcagacg
     7021 tcgtcgtcga tctttgcttg tcgccaaaat ccaattctgc ctacatggca ttagatgcag
     7081 cacttgctga tattcgagag ggcaaagctg gagatgttcc tgatcatttg agagatagcc
     7141 attacaaagg tgcgaaatca ctcaaccgtg gcgtgggtta tcagtatcct catcattttg
     7201 atcaggcttg ggtcaatcag caatatttac cagataaact gaagaatgct cagtattatc
     7261 agccaaaaga tacagggaaa tatgaacagg ctctgggtca acagtattac cgtataaaag
     7321 agtggaaaaa gcatcctcca aaaaactaga caagaaaacg gttctgtttc tgcgagctct
     7381 ctcattgctc tttttaaatt catatgctac tatagaggtg ggaaatctgt gatgttcgaa
     7441 ttgtcctctt tgtgtgttga ccgaacattt ttattgatat cttgggaccc gtcttgtgtc
     7501 actgctggta acatgcctta tcatttggta gttcgaagct acgaccatgg gacccacctg
     7561 cttgaattcg cgggatcaat actacaggac aaacaacggc attaacggag attaccatct
     7621 tttaaagaat aaagcaattt gctcttggca aattgctttt tctatttgtt gcttattttg
     7681 gaggatttgt tcatgattag gattttaatg attatcgcca ctttagtatt attattcgtt
     7741 agctattatt tatttaataa acaggatatc ttttttgtat tgatcaacaa aaatgataaa
     7801 aatcaaggat tcctacaatt ttacggagct gcatatgctg ttttaggcgt aatgggtatt
     7861 ttaacagctt ttttcaatca acgctttatt gccttggtct ttttactgat cgtaattatt
     7921 gtttctgcca ccttcagtat ccgatttgca aaaaaaattg ctgaaccaaa gcagtaaata
     7981 attctctttt tccctaaagt gttctacaat aaaagagtac aatacgattg acttgaggag
     8041 tgaatgctta tgttacaaca atacaaaaaa atcatggttg ccgtagatgg ttctgatgaa
     8101 gctgaattag cttttaaaaa ggcggtcaat gtagctatcc gaaataacgg agaacttcta
     8161 ttagcacatg tcattgacac acgttctttc caaaccgttt catcctttga tggaatgctt
     8221 gcagaacaag ctactgaaat ggcaaagcaa acattggcag attatgaaag caatgcgaag
     8281 aaagctggct tgaataatgt aacctctgtc gtagaatatg gctcaccaaa acaaattatt
     8341 gccagagaaa tcccagaaga taatcaagta gatttgatta tgttaggagc cacaggattg
     8401 aatgctgtag aacgtttatt tattggttct gtttctgaat atgttattag aaacgcagct
     8461 tgcgatgtgc ttgttgtccg aactgattta gaaaaccaat tgccagctaa agaccacacc
     8521 aaagcttaac acgtagacga cctgccaact atcggcaggt ttttttgcac aaaaaaccgt
     8581 gataaaaaca aaagacgcgg caagcactca tcggtcttta taatgtttaa aataaaggga
     8641 aaacagtcaa tgagttcaag taaaataaaa aaaagtgaac accagggcga ttagtcgccc
     8701 tggtgtggtt taaaacgacc taagcatgcc aaagcttatt ggttcgtcaa tgtggaagtt
     8761 tccgatgaat ctgatacgaa aaaatatgaa gtatcgatgg cggataccga ttttatcgcc
     8821 aaactaaaat tttatttcgg gttcgggtac cgcgagaagg caagtgctac attcggtaaa
     8881 tcattcaaga attgatgaaa ggctaccgct tgccattgca accacaacga tactcctgaa
     8941 cgcgatgttg gtgagatttc caaagagaag agctgtttaa tgcaacggta ttaagcaaat
     9001 ggcaaaagga agtcatgtag acaaagtttt ttatcaaaca ccatatgatt tcgcctgaac
     9061 gctggtttag ctcagaatac aatgatgtgg tccacgagac attaccctca gtcagataaa
     9121 aaaagctctc tgacagaatt acacatcgat aaatattaag taaatgtcag cgctagttga
     9181 actttctgac actttcaggt acactggaaa agacaactac acgaaggagg gaaaatacta
     9241 tgatgcgcat gactaaaata acaagtaatg aaaattacca acgataccat atcgcattta
     9301 gccatgcaat tggttttaca gttgctgttg acgggacaag tttgtccaaa acagttgaat
     9361 tcacgtaaaa tcaatggcca tctcaaggta tttttctcat gataatctga gtttaaataa
     9421 cacaggccat ggataaccat ggcctttttg agtggtctca aggagagaga tcatgatcat
     9481 acaagcaatt tctattaaaa aaacaatcgg cgggaatcca ttatttgagg atttatcctg
     9541 tcaaattgaa gctggtgaaa aaattgcctt agtcggtatc aacggaactg gcaaatcaac
     9601 ctttttacaa atcttgaccg gcagagaagg tgtcgacagc ggtgttatca gtcgcaaaaa
     9661 aggcttaaag atcgggacgg tcgaacaaga acttacagtt tccgaagcga ccgtcaacca
     9721 ctacttgctc cattctgccg ctgagataca ggacctcaaa cagcaaatga gtcgctacga
     9781 agcattgatg acagaaccag atacaaactt agagaagtgt ctggcccgtt acggtgagct
     9841 gcagcatcgc tttgaagaac taggcggtta cgtattagaa gaccggattg cagcgatctt
     9901 gcaaggctta gggattccac accgtcagga agcacggtta acagaactta gcggaggcga
     9961 acgagttaaa gtcgctttag ccaaaatttt agcagccgat gccgatttac ttttactcga
    10021 tgaaccgacc aatcatttag atcttaacag tattcgctgg ttggaaaact atttacagaa
    10081 tacaaaaaaa tcggttctgg tcgtttctca tgatcggcaa tttctcgatc aagtcaccac
    10141 aaaaacatgg gaactggaag aaggtgcatt gattgagtat cccggaaact acagccgttt
    10201 tcgagtcttg aaagaagctc gcttggctga gctgacaaaa aattacgaac tgcagcaaaa
    10261 agaagtccag cggttgaaag tcatgattcg tcgttttcgt cagtgggcac atgaaggaga
    10321 caatgagtcc ttcttcaaaa aagccaaaga actggaacgc cgcctggcga aactgacact
    10381 cgtcaaaccg ccacctcccc cgaaaaatcg gctgcaaagc ttgtctaatg gtggaaaatc
    10441 tggcaaagaa gttttcatca ttcaaaactt gcaccagcag tacgctgatc aagtactatt
    10501 caaagacagc agttttgcag tctatcgtgg agatcattta gccatcatcg gcgacaatgg
    10561 tgcaggaaaa tcaaccttac tcaaattatt actcggtgag gaaaagcctt acggtggcac
    10621 catcaaatta ggtagtagct tgcaaatcgg ttatttgccg cagcagctgc agtttgccga
    10681 tcccgatagc cgtttgctgg cttacgccat cacattgact ggaaacgaag aagtcgctcg
    10741 acggcaatta gccaaaagtg gcttttatca aagcgatgtg ggtaaacgga tcaaagattt
    10801 atccggtggt gaaaaaatcc gtttggcgtt gctgaaactc tttttagaaa aaatcaacgt
    10861 cctgattttg gatgaaccaa ccaatcacct cgacagctat gctcgagaag aaattgaaga
    10921 aatgctgcaa gactatcaag gaacgctcct agcagtcagc catgaccgct actttttgca
    10981 gcaacacttc caagaagcac tcgttattga tcaggagcag atcagccgcc agccgctcgg
    11041 ccaactgatt gactaggacg ttttttcgca acaaaaaact tgattttcat acctgtgctt
    11101 tcacgattca ggcaaagtac aggtggaaaa tcaagttttt attattcgat caatgaatgt
    11161 ttcggatcgg ttttctagct aaacataaag cgaagaataa agtcgacagc ccacgaggct
    11221 gatctaaaaa tgttttttct ttttacatat gtaatgtccc ttataaagat gaatgaaaat
    11281 aaaatattgg gattttcctg aaaatcaata aataaaatgc cagtcttttc ccttttgacc
    11341 acttttccaa gcaaaattcc ctaagaagat cctgtaaaaa agaagggaaa ttgaagttaa
    11401 attaccattt tgcggtcttc tccgcaaaaa aaacacatgc aagaattccc tcttgcatgc
    11461 gtttcatctt ttatcctaac gaatgctttg gcttagccgc gcagacgctc cacatcacgg
    11521 gcaatcatta gttcctcatc tgttgggatc actaatactc ttacttttga ttcttttgta
    11581 gagatatcta cttcttcacc acgcaattca ttcaacgctg gatcgatttc acaaccaaac
    11641 caagacatac ctttgatgat ttcactacgt acatgtccgt cattctcgcc gataccagca
    11701 gtaaagacaa ttgcatcaac accattcatc gttgtcacat aaccgccgat gtattttcgg
    11761 atgcggtcca cgaaaatgtc ataagctaaa cgaatatgtt ctttttcata gttcttttca
    11821 agatcacgca tatcactaga caatcctgtt aggcctagca gaccagattt cttgttcaaa
    11881 acattgatca tttcatcaat atcaatctct aatttttcca ttaaatatgg gagaacagct
    11941 ggatcgatat cacctgaacg tgtacccatt gtcaccccag ctaatggtgt gaatcccata
    12001 gaagtatcca tggatttacc tccatcgact gctgtgatcg atacaccatt tcctaagtgg
    12061 caagtgatga tttttagttc ttcgatcggt ttgttcaata attttgccgc acgttccgac
    12121 acataacgat gacttgtgcc atggaagcca tattttctta ctttgaaatc ttcataataa
    12181 gacaccggta agctatataa ataattttgc tttggcattg ttgagtggaa agatgtatcg
    12241 aatacagcca cactcaaaat gtcaggtaaa atctttttaa acgctttgat ccccattaag
    12301 tttgcaggat tatgcaaagg agcaaattct gctaattcag caatgcggtc cattactttc
    12361 tcatcgatca ctactgaatc cccataagtt tctcctccgt gaacaacacg gtgccctact
    12421 ccggtgattt catcataaga gcttaaaatt tgcagttcaa tcaactgatc taacagcatt
    12481 tttactgcta cttcgtggtt ttcgatatcc atgatttgtt cgaatttttg attatcgccg
    12541 tacttgatgg taaaaataga atcttgtaaa ccgatacgtt cgacgatccc tttcgcaact
    12601 acggtttctt ctggcatttg atataattgc catttcaaac ttgaactccc agcattgatt
    12661 gcaattgttt tagacatatg actctccttc tcgttgaatg attacagagc agatgatttc
    12721 cagtttttaa actcgttgaa aaattccgtc accttctctg gttcttttaa tgaagcaagt
    12781 ttcacaagca agacttcttt tacttgttgt gcttgtggtc cttttttctg taagatcaaa
    12841 atactttttt gcgattgttt attacggaac aattcatctg gcaattgaat cattccttga
    12901 agataacctt cttctttaaa ccattttttg atttcttcac tttgatccgt ttctaaaaat
    12961 ccactcggca tcaaaaacaa accaaaacca tccggcttca catatttcat tgattgctct
    13021 agcaataaat gatgtgcata actatggcct tcttcggtac ttgttaaaaa ctcttttgct
    13081 ttttcgtcat tcggataata accgattggc aaatcgctga ttgcaaaatc gacaggatct
    13141 attaataatt cctgcaaccc gtcttgatga aagtattgaa catttgcttg tgtcaaatca
    13201 cttgtagaag ctgcgacagc caacaatgta tcatcaatat ccacaccaat tccttctgtt
    13261 tggtaccccg cattcgatag gtttaaaaga actgttaata acaagttgcc cattcctgcc
    13321 gcaatatcca gaatcttaac cggtgctttt ttatttgtgt atagttgttc aataagaaaa
    13381 acaaataaaa aaccgatact atcaggagtg agttgatggt tcgcctgcaa atgttctgtc
    13441 tggctgccct tcaataaaag taattgagac agtctgcgcc attcttctgg ctcgaaagaa
    13501 agtttcttca actcttcata aagagctgtg atccgttgcg ttgtctcttt tgtcggcact
    13561 ccatccacca cacgtacttg ataatcatct attaaatttt ctgcattttc gacataggct
    13621 tctaaaaagg aagttcccaa tgcattctgt aaaagctgaa tagcttccag gttttgatta
    13681 aacccttgtt ccattttttc tggtaacatg gtgcacctca tttctagata tattataaca
    13741 attttttcac ctgtttaatt ctatcgaaca attgacaaaa tttcaatcgt tttcactgtt
    13801 ttttatttgt gaaacggcaa acattttttt attaaaaaca gtgttataac agaaaagagg
    13861 gcgttttcac gaacattcca tcattctgta ctagtatctg tatcattttt tacttcttct
    13921 gtgaaccgaa agataaacgg tgagacttcc acctctaaga atagggactg ttgtacccac
    13981 tgatatgtta cagatccagt tgtgtagtaa aaaactcctt ccgaactttc ggcaagcgta
    14041 gactgttccg ataaagccat ctgcttcatt atttttgcta tataataatt tttgattcgt
    14101 atattaagat ccattgacaa acggtagctt tctaatgctg ccatcaataa aaacgtacat
    14161 aaaagaaata aaagcaagac agaaaataaa atagagccct tataacattt tctactgatc
    14221 gttttttgtc catatggcat atccatcttc tccattttca aagtgtacat aaaaagaaat
    14281 tgtgtgttct ttttccaata attgaagctc agtgatactt gttaacatag gttgatgtcc
    14341 gccggaagtt cttcttttac gtatcattgc ctgatatctt tcgatgatat actgtttatc
    14401 ctcgatcgtg tagtgcagtt gctgattttt cacagagaca ttgtgactgc tcttgagttc
    14461 ttcttctaat tgtgctaaaa atattagcca ctctttttga tctttacgct gcaaaaattc
    14521 acttacttgg atggtttgct gaatcaaaag attgaatgtg agcaatagac tgctcaatat
    14581 catcagtcca attagacatt caatcagcgt aaatgctgga attacttgtt ttttcaatac
    14641 tgatcgcttc tttctccata ttgacaaaca ctttttcgat tactccatta ttatcgacaa
    14701 tttggatatc gtatgggatc atttcttccc ttcgctctgg tcttttgtga tacatccgct
    14761 gcgtcttgat atcttcataa atcattcgaa taaatagcag ttcctcccca cttttctttt
    14821 ctgcctgaag caaaaacagc tggccaaagc tcactaagta gacacagaca gaagcgatca
    14881 taagcgctgt aagggactcc aacaaaacaa atcctgtttt attcaattct ttttgtatag
    14941 cgaccactcc ctatctggaa ttgatacgta atcgtccgct ctttttcttc ccaataaaat
    15001 tgataagctt ttagtgaact ttcattcccg gttctagcag caaaagtaat agaagcatgg
    15061 cgtttcaagg taatctcatc aggaatatct aatacttccc aatttatttt atctggattc
    15121 ggaacatgaa aaatgatttg attctcttca ctattccaat aaaagcctgt ttggatctga
    15181 ttcacaatag cgattttttg cgtagaatag attcgttttt caaattggcc gaaaaattga
    15241 tagactgcca attcattctt ccatgcagaa aatgcgagaa ctggaaaaga aaggatcgtt
    15301 gtaagtaata aaatcagtaa gagtgattca aacaacgtat atcctgatgc taaaacctta
    15361 ccttcaaggc cgctgataga tttctgcatg ttctcgtttg atatagcctt cattcaacaa
    15421 ttcctccaca gtaggaactc ttccattatt ttgaagcgca tagagctctt tttgtgtgtc
    15481 taccaactgg atgatcgctt cacgactttc ttggtctaca tgattcctat aacgcgacaa
    15541 attagggacg aacaacaaca ctaatacact gatgatcagt aagacgatta gcatttcgat
    15601 caatgtaaaa ccgctataat aagatatttt tctctttttc atggcaaaac ctcctctatg
    15661 cttccgtaaa caggtaataa aacagcggca taaactaata aaatcaaagc cgctacgccc
    15721 aaaaaaatga ttggctgaat aaaatgcatc cagtgatgaa gacgtttgaa aagctgtttt
    15781 cttgtccatt cgctgtaaaa aatcagttct ttacctaaac ttccctttgc ctctccctgt
    15841 aaaacaattt tactgaattc ttctgataga aaacgataat tctggaactg ctcagccaat
    15901 gacatacctg cctccaatcc ttgaatcaac ttagaggcaa gatgctgaat cagactttct
    15961 tgtttcgttt cctttaagca gactatgatt tgccttaatt cgaaaccttc atgaaaaagc
    16021 tttccaatct ctagtgacaa ataagccgat tgatacaagg aatatatcgg tccaatcaat
    16081 aattgtctgc ttatccattc actctgatca acaggagaca acttcatcag acgatacctt
    16141 actagtaaag ccaatagagt aaccgctagg atgaatagta gaacatacca atgaaagact
    16201 tgtatcagct gaatacccca atgctcttgc atgaccattc ctgaaccaat aagctgaggc
    16261 aaaacgaaca accgcatact aaacaagata cctagcaaaa aggtgagcaa tagcatcgga
    16321 tatgcaagca gctttttcaa atcattgcga aatccttcaa caagccgaaa ctgtttggca
    16381 atttccaaca aagttgtcgg cagattccca tgacgttcag ctaactcgat ctgtaccagt
    16441 tgctctgctc catatcctat ttgcgaaagt acctcactaa acgcttgccc ttcctttagc
    16501 tgatgttgga tatttgccaa aataacaggt ggaaacgaac ggatcgtgag taataaataa
    16561 agggattctt gcagactaaa accagataaa agcagttctc ccatggtttg tgcaaactgg
    16621 ttttgctggc ttcttgttaa tcctttagaa tagttctgac tgccaaatat tttcctgagc
    16681 ttcttcataa ctttcctccc atgatttttt tactgattct tcctccataa attgataact
    16741 ccataaaaca gaaggatggt gattggcatc ttccaaaagt tcttgataaa tgacaccagc
    16801 taaacattca gatagctctt cttttttctt tgtcagttca tggattctga ccaatgtacc
    16861 ttcaagattg cgtgcatgta cggttgcaaa aacccggtgc cctgttagtg ctgctcttac
    16921 cgcagcatgt gcagttgatt catctcttat ctcgccaatg attagcaagt ctggacgatg
    16981 ccgtaatgcg agtctcaaaa gtgtatcata agtctgtgct attttcggat tgatctgtag
    17041 ctgcagaaaa aacggttctt ctatctctac aggatcttcg atagtaatca cttgcaattt
    17101 ttcttccaaa gccaaccgat acattagaga agtcttgcct gatccgactg gtccgctgaa
    17161 aaggtataat ccacgtctga cggttttctg taaaatagtt gcaaaatctc gagaactaaa
    17221 gcaacgatgg ggtgtttcaa aagtaccgta taatagccga atcactaaac tctctcgttg
    17281 aaggtaatct cctacagaag acaaccgcag acgaacttcc tgtttttcta aagaataagt
    17341 aaatgcacct aactgagctt ttcttttttc tccgatatcc atctgtccca aatacttaaa
    17401 tcttgagatc agttgctgtc catctgccga agagactttc ttccagaatg ttcgtttttt
    17461 tccatagcga aaatagatgt aaaatacttc tgaagctggc aatatatata cgtcttgggc
    17521 atgtttggca aaactttcac ttatcatttg atccgataat cgttttattt ccattattct
    17581 cccctccaca aaaaacatac gtctttttga tctttttgtt tcaaataaaa tgaatttaca
    17641 aaaaaatatt ggcaaaaacc gaatttatgt tatacttata aaaatttatc ttgggaggca
    17701 ctccattgga aacaacatta aggaaatata tcgaagtagc aagtaaaaaa aagcctgctg
    17761 acctagtgat caagcatgct aagatcgtca acgtctttac gaaagaaatc atggaaaaag
    17821 atgtagcaat ctgcgaagga atgatcgtag gaatcggtga atacgaagga aaaagcgtac
    17881 ataatgctaa tgggcaatat ctagtgccag gattcatcga tggccatgtg catatcgaaa
    17941 gctcactttt atcaccaagc gaattttcaa aagtatcctt gcttcatgga gttactactg
    18001 tagtgacaga tccccatgaa ataggaaaca ttgctgggag tacaggtctt gagtttatga
    18061 tagaagatgc aagacaaaca ctcatgaata tcttttttat gctaccttct tgtgtacctg
    18121 taaccccttt tgagaccaat ggtgcaaatt taaatgcaaa aaaattagcc ccttttttac
    18181 aaaaacccga agttttaggc ttagctgaag tcatgaacta tcaagcagta gcaaataatg
    18241 aaacagacat cctagaaaag atccaattga tgcaccagca taagaaaaaa attgacgggc
    18301 atgcagctgg tatcggcatg gaagagttaa atgtttatcc tgcagcaggt attcggacgg
    18361 atcatgaagc tacaactgca aaagaagcta aagaacgttt ggatcttggt atgtatttaa
    18421 tggttcgaga agggaccgtt gcaaaagatc tggcatcgct actaccggcg atcacacctg
    18481 aaaattcaag aagatgcttc tttgtcaccg atgataaact gatcaatgat ttagtaaaag
    18541 aaggatcaat cgatcatatc gtccgtcaag ctattgcact tggaatcgat ccattacaag
    18601 ccattcagat ggcaacattg aatgcagcag aatgttttga tttgaaaaca ctaggtgcga
    18661 ttgcccctgg gtatcaagct gatttcttct taacagacga tcttaccaag ctgccaattc
    18721 atgaagtttt ctataaggga acattagttg ttcaatctgg gcaactccaa gaccatttat
    18781 ttgctgatca ttcaaattca tatacgtgtt gtctgccaaa actaaacgct caaccattaa
    18841 atccacattc gttagagctg cttctttctt cttcacaagc ccatgtgatc gaaattatcc
    18901 cgaatagtct agtgactaaa gatttggtag aaaaagtgga ccgacaaggc gaacgattca
    18961 ttccttcaat cgaaaaagac caattaaaga ttgcggtgat cgagcgtcac catcaaacgg
    19021 gaaacattgg attaggcata gtgaaaggat ttcagctgaa acaaggagcg atagcgacaa
    19081 cgattgccca tgactcgcat aatctagttg tcgttgggac aaacgacgag gatatgctgt
    19141 atgctgctaa tcttttgatt caaaaaggtg gcgggatgac cgttgtcaac aaacagcagg
    19201 aaatagcttg tgtttctctt cctataggcg gaatcatgac acaggaacct ttttcgatcg
    19261 tctatgaaca gttgaatgaa ttaataacta aagcacatca actaggagca gctaaaacct
    19321 ttgatccttt tttgacactt tctttcttaa cattatctgt catacccgaa ttgaagatca
    19381 ccgacaaagg attattttca ttttctcgct tccaattgat tccaccttgt ggaaaaaccg
    19441 atgaaataaa tacattttaa atcaaataaa tcccagaaaa tacattacat ctgtaatgtt
    19501 cattttctgg gatttattta tggatattaa tgtttttatg aaagcagaaa gttagtttca
    19561 cagacttttc tttttggtct tatcttgaaa ttgagcttct actcgataaa tacgttgacc
    19621 ttttgaagaa aatttttctt catactccgt cataatattg ccttcaaatt gactttgatg
    19681 taaatctaac catacctgtt tgaggatcat gccatattga gaaaaacttg ccagagaata
    19741 ctcaaacaat ccttgattat ctgttttgaa atggatttct ccatttggac gaagaatctg
    19801 ttcatctaca gcaagaaaag tcttatatgt caatcgccgt ttttcatgac gtttttttgg
    19861 ccatggatcg gaaaaattca gatagatttg atctacttcg ctgtcagcga aatactctgt
    19921 caacgctgat ccatccacat gtagaagttt caggttagga agcggttgtt cgatcaactt
    19981 gtctagtgca atcgaaacaa cactgacctg catttctact ccaatataat tgatttcagg
    20041 atgggctttt gccattccag tgatgaactg gcctttcccc ataccaatct caatgtggat
    20101 tggatgatcg ttttcaaaca attcattcca ttttcccttc cacaatgttg gatcactaat
    20161 tacaaaattc gggtgagccg ctaacatttc cgctgctccc ggacgatttc ggacacgcat
    20221 tatttatctc cattcctttc ctctaattaa aaaggtctgg ccaaatctcc gcagagtttg
    20281 ggccaaacct cagccactga aaattcgttt taattgtaaa atttcttcgt tcatccgtcg
    20341 atgatcttca cgtttgcaat atctggtgat ttcttggagc aaattcatgg ccgcatacca
    20401 atgaaatttt tcaagcattt cgtcagtcgg gcgtatgcca taactgatca gccactgatt
    20461 ccagctagac agtggcacat aatttcctaa aatcgttgca atatccaccg cagggtctgc
    20521 aaacatgatt gaatcccaat cgactagata taaataattt ttacatacta accaattacg
    20581 atggtttaca tctccgtgta ctactgataa gttttcctta tctattgacg ggatctgctc
    20641 acgaagaaaa cgatggacaa tttgcagaaa ttgatttttc tggagttctt ccggtaattc
    20701 tttttcatat ttttgcagca tcttctccgg cgtcatgaca cgaccaccga tacgggtcag
    20761 catggtttta agagaatttg aatgatgtaa atggtacaaa acatcaatca cgtcgtttct
    20821 ttgaccaatc tcattaggag tcagcaagcg accttccagc cactcttgtg ctgtcaaagt
    20881 atcgccattc cccgttcgtt ttgtccaaac aagttttggt gtaattcctt cccttgaaag
    20941 agcagccaac atcggagtcg tgtttcgttt aataaagact ttgtcctcat cacgcatgcc
    21001 tatgtatgtt tttcctgtgt cgcctttgat gggacgtaac cgccactctt ggtctaactg
    21061 aaactccatc atgcattctc ccgtcctctt actctttgtt ttgcagtact tattattcta
    21121 gtcgcctgaa cactacacgt caagcacata tacgttcttt ttcatttaat tgccattcaa
    21181 aaaaccagct tttctcaaaa aaaatagaac gaacagcatt cttactgtca acgctctatt
    21241 tttcattctc atttttaccc tacacagctt tgttttcagt ctgacatctt cttcaaacga
    21301 gttggtacat atagtttaac aaagagcata gtaacaatca aataagccgc tagcggaacc
    21361 catcgctgct gcccatctaa tacgactgca gcaatcaaac caaaaataac cgacgcgata
    21421 attaataccc atgtgagtag atactgaatc gcctgcttct tctgattctt atggattgga
    21481 taaagctgaa cgagaaccat atagcggaac tggttcgcta aaggtaacag ttggaaaccg
    21541 atcaaatata aaaataagaa accgataatc agactaaaat aaaagtcgac tactccggca
    21601 atcaataacc cacctataat tactaatcgg aaataaagac cactaaaatc agaacctctt
    21661 gccaatcttc ttgcaaacaa gtaaagataa gtgttttttg attctctttt aatatgagtc
    21721 aatataggat ccaaatagcg tcttcttttg atctttgccg taatttcagg aacatctgtg
    21781 aataaattga taaattgata gatctgatgc aacctatgtt cttctacatg caccattttc
    21841 tcccaatcca atcctttttc ttgttgcttc cacaaaaacc agtaatatag acttgcctgt
    21901 actaatgcac ctgctattcc tgcccatatt agccaataaa atgatagaaa caaaaagatc
    21961 agagaagaaa aaaaccagcc taatttccaa taacgaataa cttgttgatc tagtcgaaac
    22021 aattctgctc tcttgatttg cagatgactg aatttcaaac tccataatat gatcagatag
    22081 acgaaaaaca tcgaaaagga ttgctccgta ctaaccacca ctaatggcat ggccaatccg
    22141 catgccaata tcaaaaagat caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa
    22201 taggtttgca tttctttttc tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa
    22261 gtagccatct tacccacgaa taaacagagc atccaaaata cagagattat gagccctcca
    22321 attgcaaacg gtggctgcag ggttttcaac caattagaat aatataatcc cacaccgcca
    22381 atcaagaaaa gacaaactaa aacaaaatga tcgttcaata cataacgcat atatttcatc
    22441 attttctttt gatgtcttgc taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt
    22501 tgttaaagca atatagattt cgtccaaaga agaatccggc agctgaaatt cttttctcaa
    22561 catctcaaga gaaccgtctg ctcgtatctc tccgttatgt aagaccacaa aacgatcaca
    22621 atatttttct gcagtagcta aaatatgcgt agacatcaaa ataccggctc cttctttttt
    22681 catttctacc atcaattcaa gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg
    22741 ttcgtcgatg atatataaag aaggctcgat caaaaaggcg cataagatca tcactttctg
    22801 tttcattccc tttgaaaaat tagctgggaa ccagtcaaga cgattagata accgaaaagt
    22861 ctccaacaac ttttctgctc ttttcattgc tatatccata ggtatatcgt aggccatcgc
    22921 agtgacttcg atatgttctc tcaatgtcaa ttcttcatat aaagaaggag tctctggaat
    22981 atacccaatt tgtttgcgat attcttccgt atttttcttt aaggatagat gattgatttc
    23041 tatcgttcct tttgaagctt ccagcagacc gataatgtgc ttgatggtcg tacttttccc
    23101 tgctccgttc agtccaatca accctactat ctctccaggc tgtacatcga aactgatatc
    23161 ttttaacaca gggagatgtc cgtacccccc tgtgacatga tcgataatta aactcattct
    23221 ttttcctccg ccatttattt ttatttatta taccacgaag aaaatgacgg tattttgttt
    23281 ttttccttac aatagcttta acttatggta aagttaagct gaagtattta ttctttatag
    23341 aaaggatgtt ttatatggaa aactgtattt tttgcaaaat catcaatcaa gaaatcccaa
    23401 gctataaaat atatgaagac gacaaggtct atgctttttt agacatctct caagcaacga
    23461 aaggacacac attagttgtg cctaaacgtc atgtagcaga tatttttgaa tatgatcccg
    23521 aacttgcagg agaggttttt tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt
    23581 ttccagaaat gaaaggtctt aatatcttga ataacaaccg tgaactagcc tatcaatctg
    23641 tttttcattc acatatccat ttagtccctc gctacagcaa agaggatgat ttttctattc
    23701 actttgtcaa tcatcaagat agttatggat cggaagaact aaaagctatt caagaaacaa
    23761 tcgtaaagca ggtgtcctgc gatgattaaa aactttttca aaggactgtt atttggagct
    23821 gctgctggca ctgtcggagg tctgttattg gcgcccagga gcgggaatga aacaagaaag
    23881 aaactgatcg aagaattgga agaagcaaca actctgacaa atgatttaaa taacagcctg
    23941 aatcatttta aaagcgcatt gatcgaaaca aaagaaacag ctgaatctgt gattcctgct
    24001 ttccaagaag caatcaaaaa agatatcgaa gattttaaat tccaagccga accacgtatc
    24061 acacaaatca atgaacaagt agaaaaacta aaagctgatc tgccagagta tcctgaacaa
    24121 atataataat gattattttc tcaacagatt taactatata agtgagaaaa gtttgcgtat
    24181 ttcctaggaa tgtgatgaaa ttttgaattt tgtttgaaac taccacgaca acgtttttaa
    24241 aggaaatggg atatggtata gttaatatat aaaaataata actaattaca ggagtgcaaa
    24301 ttttaatgaa aaaaaaatca attattttgg cagctactag tgcgttggct gttttaactc
    24361 tagcagcttg ttcaggcgat actaataaag atatcgcaac gatgaaaggc ggaacgatca
    24421 ctgtatctga tttctatgat gaagcaaaac ttgaatcatc gaaccaatcc ttagtacaac
    24481 ggatgatcat ttataaagta ttcaataaca aatatggcga taaagtaacc gataagcaag
    24541 tggatgcaga atacgacaaa caagcgaaat cattaggtga tacttttgaa tcacaattag
    24601 aagctgctgg atatacgaaa gatacttata aagaatatat tcgtaacaat ttagcttttg
    24661 aagctggctt gaaagctcac gtagatatta cagatgacga tttgaaaact gcttggaaat
    24721 ctttccatcc agaagtagaa gcacaaatca tcaaattatc aagtgaagat gaagcaaaag
    24781 atgtgaaaaa atcagcggat gatggagatg atttctctaa attagccaaa gacaaatcaa
    24841 ctgatacaga gacaaaagaa gatggcggta aagttaaatt cgactcaacg acaacaacta
    24901 ttcctgcaga agtaaaagaa gcagctttca aattgaaaga cggtgagatc tccgatgtta
    24961 ttactacaac aaatcctact tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa
    25021 accaaaataa aggcaatgac atggataaat acaaagatca actaaaagat atcgctactg
    25081 aaactaaact aagtgacaat gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg
    25141 caaatgtaaa aatcaaagat gatgcctttg aaaatgtgtt gagtgcattc acaacaactt
    25201 catcaagtac gaaagactca agtgaaacta cagcttcaac aaagagttct gacacaaaat
    25261 caactgattc aacaaaagaa tcttcaactg aagaaacaac tgattcatca aaataaaaga
    25321 aaacaaaaaa agcgaagact tatgtctcgc tttttttgtt ttgaatgaaa aaaataaatt
    25381 tttgctataa atcttttggt acatagaaac ttcggttgtc cataccgaaa atccgatcgg
    25441 tgtattttcc tggttctgtt tgacgaatcg aagtcaacat catctgcatc gaagcatcga
    25501 tattgtcgat ttgatgtaat atttctgctt ccattattct tggacgtaca ggtgagccat
    25561 attctaataa gccatggtgt gctaaaacca tatgacgtaa tacgagaaca tcttcgtcag
    25621 cttcgctgat tttcaacgca agacaggctt tagttatttc ttcgtcaatc aataccagat
    25681 gaccgatcaa atttcctgca agcgtatatt ctgtcgacat aggaccggaa agttcaatca
    25741 cctttcctaa atcatgcaaa atgataccag catacaacaa tgctcgattc agctcaggat
    25801 attcatcggc gattgatttc cctaagtgaa gcattgtcaa agtatgataa gctaatccac
    25861 tggcaaatgc atgatggttt ctttttgctg ccggaaactc aaaaaattgt ttttgatatt
    25921 tattaagtag atagcgaaca atacgattcc aatgcgcatt ggtgatctca aacaacaatt
    25981 gattgatttc ttcttccatt tcttctttct tcaaaggggc tctttccatg tataaactag
    26041 gctctcctgg ctcatctacc cttgttaagc gcatatgaag aatctttact tgagggtttc
    26101 cttgataaac ctctctttta ccgtttagaa aaacgactgt gccggctgta aatttattga
    26161 tttcctcctc cgaagcatcc cagtatttac catcgatagt acctgaagta tcttggaaag
    26221 taaaagcaat aaattttttt ccgtttttag cgattcttac atctgcactt ttgatcagga
    26281 caaattgttc aaactgctca tcgattacta aatcacgaat ttttttcatt atttgctcct
    26341 tctaattgtt ttacgatttg gttcatctca tggtaatagc ttaccatttc tttatcagaa
    26401 gaaaaacaaa ttatttgata cttttctcca aatttagcta aaagttcagc taattgatac
    26461 tttcgctgat gatcataatg aagccagcca tcatcaatga tgatcgggca tatcgaacgg
    26521 ttagcttcta atgagaggta agcaaatctc atcgccataa tcagctgatc tttcgtacct
    26581 gtagataact catagatggg aaattctgta tatccgtctg ttaaagtcag tgtatcattt
    26641 ttcagtaata tcttctgata atttcctttt gttaaaatac ctaaataatc acctgctgct
    26701 cttaacaact ggggtaattg tctttcactc atctctgttg aaagatcacc taagaatgtc
    26761 ccggcgattt caagtgctcc ccattcctcc agtaattcct gtaattttgc tttttgccgg
    26821 ctagcttgtt gataaagttc gtccaatgtg ccgtctcttt gcaactcttc tacttgaagt
    26881 tggagtcgtt gccgattttc acttgcttct ttttcttctt gctggagttg ggattgcttt
    26941 tctttcagtt ggcgcagtct tacagataat ttctcttttg tcagctcttc aggaaatagt
    27001 ggagtaagca tttccgataa ttcattgtat cttttcatct gctgttcggc ttgtttggct
    27061 ttttgtaaaa agacaggtac ttccgaagga taagataagc ctgcttgagt caattgttcg
    27121 ctgtattctt gtatccatac tttttgtttc tttttcaatt gattgatttc ttgcttcaat
    27181 aaagtatttt gctgatagct tctggcaaat ttcatctgtt ccatctcttg tataaaacgg
    27241 gaaagcaata ccattttttc tgaaattgtc ttatcttgta tcggtaacca ttctttaaca
    27301 aactcaaatt tttggtcgat ttgtcgtagt ttttcttgta tttcctgttc tcttttatgt
    27361 ttccgctcaa atacgccata taatttctga taatctatga cttcctgtcc atattctttc
    27421 atgagtttga gcgtatccat ctttcctaag tgccgatgat ccgcaaaacg atatacttct
    27481 tgctgaagaa tctgtttttc ttctccagtt ctcttttgtt tttcttctgc ttcagctaat
    27541 tgagctaagg ctagatcaag ttgtcctagt ttttcttgcc acattccttt tatctcagag
    27601 aaatcatttt tagttttaat ttttggttgc cttttgtaga agaaatagcc aatcatgcct
    27661 gcaccaatta gtaaaaggat cactttcaag ggcattggta caaatatacc tgcaatgatt
    27721 attgctccgc cagccatcag catatacggt ttagatgaag agactggtgc tatctctgac
    27781 tgattttgta ataatgctgg ataggtctct tccaagtcat tcacttctgc ttcaatcatc
    27841 tcttgctgtt ctttagctat ttggagacgc acagaaatct ggctttctag atcagcaatc
    27901 cttctttctt cagccagaag atgcttccat tcttcttcat ataaaaattc ttccggtaac
    27961 ggatttttct gccaatgcca cctttgttcc aaaatcttca attctgaatt tgtttctcta
    28021 gtctcaattt gtaatctttg ccattcttcg tttaattgtt ccgctgtaaa ttgaagttgc
    28081 tggagatctt ttagttcctg ctcctgctct aaataaaaat aatatctggc tgactgctga
    28141 tccatcccgc tatgcttttc cagtgttgct tctaattctt ccaaacgctc atttgtttgc
    28201 tgataccgtt tggagaaaga ctccagatct aaaagaagtt gctcatcagg aagacctgta
    28261 gtgctgtttt ttttgagaga ttgccattct tcatataaag aaaaattaag ttcttgctgt
    28321 ctggctaatg aaaattgttt gttcgcctct tttaattgtg ccaataactg ctgctgttgc
    28381 gactcgtatt cctgttcttg ttggatcaac ttgccaaaat aagcttcctg ttcttgtttt
    28441 tgatgaattt tttcttgcag ctctttccat tcatttaatt tttgattgat tagtaatttt
    28501 tggccttttt tcttgaataa ttgttgtgct ttctgataat attcttgccg cttttggaat
    28561 acttgagaac tgcccgtgat gcctaaagaa atcagcgcat catgtagctc tttttctctc
    28621 aaatgatcta gttccatcaa ctgctcttgc tggaaagtaa atacctgctg gaataactct
    28681 ttcgtcaaag gtgcgatcag tttttctagt aactcatcac ttcccacttg atcccccagc
    28741 cacaccttcg attttccgcg attttgctgc ttgtagcgct cgacagcaaa ttcaccatat
    28801 acaggatgtt taagccatat tttgccgcca taggctgagc cgtcttttgg tgtataatcc
    28861 ttttttttac ggccttttga agggaaacca aataatattg cttgaataaa ttgataaatc
    28921 gttgattttc ctgcttcgtt tcccccatag atcagctgat tgcctgaata aaagtcaaaa
    28981 cttttttgac gccattttcc aaaagcagca atctcgatcc ttaaaatctt cattcctgat
    29041 cctcctcaaa taaaaaatct tgattaagca gctgcttggc tttttcaagt gtttcttctt
    29101 gatattccgg cagttcgctc aatattctag ccgcttcttg atgactatat acttcattta
    29161 aaatttgttg gaaatcttga ggtgtctgat agtattgaaa taactgttcc atcaatttag
    29221 cagaagcagc aagtggggtc ttcgtataaa aagtattctc tatgagagaa atacgccaaa
    29281 taaagaaatc atttatccta tctgagaatt cctctaacaa atagtctaat agctctcctg
    29341 attgtatccg ttccaaaaca tctgtattga gatggtcata atcttttaat tttatctcaa
    29401 ttaaactaaa tccatcctct acttttgaaa gctgctgacg aatgtgaaca atgacctctt
    29461 ttgtcgttct tgctttcctc aatgatattt cctttgtctt ccaatagacc tctgctactt
    29521 taatcggatg tatttggcaa cttcctttac ttaattcaac tagtagaata gatgtagaag
    29581 cttgttcctt tttagtatga ccttgaggcg cacctggata aacaattgtt tgcttttcat
    29641 ttaagaccgt tggcacatgg atatgcccta aagcccaata atcgtatccc ttttcttgca
    29701 tctttgaggg ttggaaagga gcatagtttc ctttttgaga gacgccaggt tccccgtggt
    29761 acaacccaat atggtaatct gtgagttctc tagaaggaaa ttccatcact ttgtcttttt
    29821 gaatccacgg ttgacgataa ctaaatccac tgagagagac agtttctcca ctttttatcg
    29881 ttattttttt tgtctctacc gtttcggatg taaataaatg gacattttta ggaaaggaga
    29941 accaataccg ctctttttga tagaaatcat gatttccgaa tattataaat acaggtatat
    30001 ggttcttttc taaccgtttc atctgttctg aaaaatgttt ctgtattttt aatgaaggac
    30061 gattttgatg aaaattatct cctgcaagta aaacaaaatc aacgttattt attattgcct
    30121 gttcaatgat atttgataaa acggtcagat tcgtcttcaa tagtttttcc tgtacagttt
    30181 tatcaagagt tgtcagtcct tcaaatgacc gatccatatg taaatctgcc gcatggataa
    30241 atttcaccat taaaaatttc ctcctttttt tcttttatca taccattttc aatgtttttt
    30301 ttcatcaata accgaaaatt tgttcgtatt atgcaaagaa attacgataa atggccgact
    30361 tcaaaaaata agtttattcg aaaaaaagat tgtgacatga tttttgtcac aatcctaaat
    30421 atctatgtta tattcgcttc gtttgacact gaagaagtac tatttgtact ggaatttatg
    30481 ctccattttt ccgtatttag aattctatgt tctatactac cttaattagt ttatatggta
    30541 agaattcttt caaatgcttg agatactccg tctttatcat ttgaaacagt gacataatca
    30601 gcttttttca tgatttcttg atgactattt cccattgcaa aactaatatc tgcactttca
    30661 aacatagata agtcatttaa actatctccg atcgcgattc tcaaaggttt ctttaaattg
    30721 tagattgatt ccaaacgatc aaacaccaca cctttagata cattttgagg tacgatttca
    30781 atatttacag gtgccgattc agtaacagac acatttagca tctttttcaa atggttaaat
    30841 atattagctt tcaattgacg attaggaata aaaataaaaa atttgtaaat gctgatagca
    30901 tccttttcta taatttcctc aatattatta taagggattg aacaaataga tttttcatac
    30961 tcaagctcat agtttagctc tttgatagaa aattcactcc ctacctcgtg aaaaattttt
    31021 ttcattttat ttttataatt ctgagattca taacttccga gatttgtata tattttggtt
    31081 ggaaatttcg ataggacaga atcaattttt tttacatcat ttttatcgaa ggaagactca
    31141 aatatctttt tttctccatc aaatccaaca gaaccattta accctactgc tggtatcctt
    31201 aaaccagatt ttttgagaat attattaatg tcttctactt ctcttccaga acaaatgaat
    31261 ggaaaacagt cttccggtaa atgtttcatt gttcttgcat ttttttgact aatgctctgg
    31321 tcagaattca aaagagtacc gtctaaatct ataccaatta gtttcatttc atccatgtct
    31381 cctatacgtt gattataaat tcactgctta ttgtaagtct aaatttctca caaaacatat
    31441 actaattata cctaatttaa gtaagtacaa acatcatttt aagaatagga aaggacattt
    31501 atatactatt ctctgtattt ttgatttata aataaatatg aattgaatcc aaatcagcta
    31561 tattactatt ttataataac caactataaa aaacgaagtt aggtgaaaaa ctaacttcga
    31621 tattcataaa agactctatt agttcaactt agcaaaagat ttaaacaaaa a
//
LOCUS       DACCQI010000014        31627 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCQI010000014 DACCQI010000000
VERSION     DACCQI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.014"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(113..556)
                     /locus_tag="IVO76_000537"
     CDS             complement(113..556)
                     /locus_tag="IVO76_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7428526.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(572..1732)
                     /locus_tag="IVO76_000538"
     CDS             complement(572..1732)
                     /locus_tag="IVO76_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7428527.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2239..3939
                     /locus_tag="IVO76_000539"
     CDS             2239..3939
                     /locus_tag="IVO76_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428528.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(4041..4874)
                     /locus_tag="IVO76_000540"
     CDS             complement(4041..4874)
                     /locus_tag="IVO76_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7428529.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5178..7343)
                     /locus_tag="IVO76_000541"
     CDS             complement(5178..7343)
                     /locus_tag="IVO76_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7428530.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7603..9897
                     /locus_tag="IVO76_000542"
     CDS             7603..9897
                     /locus_tag="IVO76_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7428531.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9890..10555
                     /gene="pgmB"
                     /locus_tag="IVO76_000543"
     CDS             9890..10555
                     /gene="pgmB"
                     /locus_tag="IVO76_000543"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7428532.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10548..11555
                     /locus_tag="IVO76_000544"
     CDS             10548..11555
                     /locus_tag="IVO76_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7428533.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11577..12596
                     /locus_tag="IVO76_000545"
     CDS             11577..12596
                     /locus_tag="IVO76_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7428534.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(12877..14244)
                     /gene="nhaC"
                     /locus_tag="IVO76_000546"
     CDS             complement(12877..14244)
                     /gene="nhaC"
                     /locus_tag="IVO76_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7428535.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(14353..15774)
                     /locus_tag="IVO76_000547"
     CDS             complement(14353..15774)
                     /locus_tag="IVO76_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7428536.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(16017..17894)
                     /gene="tdc"
                     /locus_tag="IVO76_000548"
     CDS             complement(16017..17894)
                     /gene="tdc"
                     /locus_tag="IVO76_000548"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7428537.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(18185..19441)
                     /locus_tag="IVO76_000549"
     CDS             complement(18185..19441)
                     /locus_tag="IVO76_000549"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7428538.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            20021..21322
                     /locus_tag="IVO76_000550"
     CDS             20021..21322
                     /locus_tag="IVO76_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7428539.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(21391..21990)
                     /locus_tag="IVO76_000551"
     CDS             complement(21391..21990)
                     /locus_tag="IVO76_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7428540.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            22244..23125
                     /locus_tag="IVO76_000552"
     CDS             22244..23125
                     /locus_tag="IVO76_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428541.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            23138..24748
                     /locus_tag="IVO76_000553"
     CDS             23138..24748
                     /locus_tag="IVO76_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7428542.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(24825..25475)
                     /locus_tag="IVO76_000554"
     CDS             complement(24825..25475)
                     /locus_tag="IVO76_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7428543.1"
                     /translation="MCFICSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPNGEP
                     QLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTEICI
                     LRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLAKAT
                     GKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLEDL"
     gene            complement(25775..26440)
                     /locus_tag="IVO76_000555"
     CDS             complement(25775..26440)
                     /locus_tag="IVO76_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428544.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(26587..27192)
                     /gene="dhaL"
                     /locus_tag="IVO76_000556"
     CDS             complement(26587..27192)
                     /gene="dhaL"
                     /locus_tag="IVO76_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7428545.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(27220..28203)
                     /gene="dhaK"
                     /locus_tag="IVO76_000557"
     CDS             complement(27220..28203)
                     /gene="dhaK"
                     /locus_tag="IVO76_000557"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7428546.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(28205..28600)
                     /gene="dhaM"
                     /locus_tag="IVO76_000558"
     CDS             complement(28205..28600)
                     /gene="dhaM"
                     /locus_tag="IVO76_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7428547.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            28754..29074
                     /locus_tag="IVO76_000559"
     CDS             28754..29074
                     /locus_tag="IVO76_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7428548.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(29186..30055)
                     /locus_tag="IVO76_000560"
     CDS             complement(29186..30055)
                     /locus_tag="IVO76_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7428549.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(30151..31455)
                     /locus_tag="IVO76_000561"
     CDS             complement(30151..31455)
                     /locus_tag="IVO76_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7428550.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
BASE COUNT         9232 a         6600 c         5730 g        10065 t
ORIGIN      
        1 tatcaaagat tgtaacaact gcatattcac acagttaaat tagtgacttc aaaatgaaat
       61 aatgctccca gcaaaaaatt aggctgggag cattatttcg ttattctgtt catcaaaaat
      121 cagcgttttg attccatttc atcacaagtt cctcctcatt tactgcttca tctttttgct
      181 gtgtttgaat tgtaaaacct tgtgaaagat agaaatgaat agctgattga tttttttgat
      241 agactgtcaa agtcagtgtt ttattggttt cttttatttt tttcattagt tgcgtaccga
      301 ttctttgttg ccggtattcc cttttcacaa agattcctgc gataaactga tcaatcaccc
      361 cagcaaaacc aataatttct tgatctattt ctgctaagta aatcactgca tctggtaaag
      421 ctttcctaac agattctact tgattctccc aataagttga tggaatgaat tcatgagaat
      481 ccaagttagc atgcagccaa atcgatagaa cagattccag ttctttggca gacaattttg
      541 ttgttgtgcg gatcattttt tctctcctta cttattcatt gattttaaaa taaaaaccat
      601 ttttttgaag ctcttttaca agttttcgcc cctcaaaaaa gtatatcttc tcttgtttta
      661 cttttctaat accaaaagaa acagcaactt taggatcatc agctgaaacg attcgataag
      721 ttccaccagg caattgatcc agcttgactt tcccatgatg gtcgctttgt actgaaaacg
      781 gcacaccatt tctagtaagt gtttttttct tatgtttcaa aatgaatgaa ctaccattta
      841 ccggtttccc tgacgaatca ctaataataa atgaaaaatc aagcactttt ttacttaatc
      901 tatactgata gattagtcgt ccgataaaca ctaacagtat gactacagct attatcgttc
      961 ctgcaagaat aaaccattgc cgcatggttc tttcctgatt tccttttcct tcttcttttt
     1021 ttaccgattc gttataagga atccgatgtc ctgtcactag caaacgatgc gtgttgatca
     1081 tatacggcgt acatgtaagc aaggtaacta agtcttctcc ttctgaaatc gttagaacag
     1141 acgtgtcatc tggcagtacc acttgtatat ttttaacttg ataagccaat ttctccccca
     1201 aaacagtcaa aataaacgtg tctccgtttt ctaaacgatc taattcggta aataaccgtc
     1261 gattaggtaa accgctgtga gcagagatca ctgaatgcgt cgaatttcca ccaagcggga
     1321 aagaggttcc ttgcaagacg cctgctccat tttcaaggat cctaccattg agccgatcat
     1381 aaagtgaaat atttaattgg atcttaggta tattgatact tccgatcagc cattcctcta
     1441 attggctgga tgttgtactt tcatttctgt tcttttcatt gaaaggatca gtaccaggat
     1501 tgaatccttg gtcagttaat cgttggtttt tctcctccat tttctttcgc tgcacttcaa
     1561 tttcagctgc ggtccgtttt tgtacttgtt cgatacgttt ttgatcaata aaactattta
     1621 aagaatccac ataaaatgga tataaagcga tcagtaaccc tacgacgata catattaata
     1681 aaaacaaacg aaacaaaagt cgacgagact gtttttgctt actctgcttc acatgtgtcc
     1741 tcccttgcaa gtttttttgt tctttctttt attatatggg ttcaggaaaa aaatagaaaa
     1801 tgaaagccga tgaggttgtg aaaaagctgt cctgcatcaa gtaataagta cagcccaaaa
     1861 aaatagctac tcatattttt tgttgggttg ggcttattac cgcagatgca agcttttgaa
     1921 caccgtttaa ggaagaggtt gtgaaaactg cgttatgctt tgagtaataa gagcgctagt
     1981 cagaaaatag cttctcatat tttctaggct agtgagctta ttaccgagaa gcagctgatt
     2041 gaacaccgtt taccgagagg tcatgaaaaa gctactttgt ttcgagtatt aagaaaaatt
     2101 ctgtttcaaa acaaggagat gcaacaaaac ttatgtcgca tcctctctcc ttagaacttt
     2161 cacttatgtt tttcttgtaa aattcataaa tattttcatg aattttctaa tttcttgcct
     2221 acctctcgca taggatatat gatgaattta tctaaagttt gcaaaggagt tcgacaattg
     2281 aaaaaatatg gcccattttt agcaattttt ttcattgctt tggcatttct catgcctcat
     2341 ttattcacac ataaactaat tttgggtgct gatgccccat tccattacaa tcggttctat
     2401 gatgccgcgc aacaaattaa atatggggat ttccattatt ttccttccct ttatggattc
     2461 cagcaaagtg gcaggatcgt gaatgccgtt tacggcccat ttttcgctta tttacaagga
     2521 ttgatcgtgt tgcttgcagg tagctggttt cattatcaga ttctttcaaa tttcattctt
     2581 tatcttattt caggattttc tatgctgatt ttactgagat atgtcaatgt tcgaaattgg
     2641 gtaagtgtac cagtagccgt tttattcatg agtacgtatt ctattcaata ctggtctatc
     2701 aaccaaggat tcactagctg ggggacttgt ttttatcctt tatgtatgat ccctattgct
     2761 gatttcgtcc ttcataaaaa gttccctgtt atcaaagtcg ctgtctcagt gggacttatg
     2821 acacagatcc atttactgtc ttcgatcatg ttagctttga tgtatatccc tttttatctt
     2881 tattactttt ttaaccagac tgaaaaagga aaagcactgc ttcaactgat taagtcggtc
     2941 ttgctttatt ttgttctgac agcaaatatt tgggcaggat tagctattgt ttacagtggg
     3001 aataagattc ttcctccctt tgttaacaga gaaatgagtc aaaaggcgat agatctggat
     3061 ggcagttatt ggctccatta ccctaaggcc tttcctatat tgatattatc aggttttttg
     3121 ctttttattt tctttcaaca aaagaacacc ttattccaaa aaatcgtgtt gtacacaagt
     3181 cttgttttct tttttctttc tactagcttg attccatgga cgacattgat cgaaaaaaat
     3241 gtgcctttta ttagtttgat ccagtttcca tttcgatttt tcgtgccatt tacgctattg
     3301 cttctacttt accttgccct ttcactgaac cagtggtctg aacaaaagtg gttccgtctt
     3361 ttaagtatat ttggcattct tattttttca gcacagacga tccagaactt ataccagcat
     3421 ctggaaaagt gggaaaatga aacatttgtc agtcgacata cttatctatt cgatacgccg
     3481 gaagaagcaa ggaaaacatt cttcagcaaa gatatgtcta ccagcttgtt cgttttccaa
     3541 aaaaccacac ctgactatct gccaatctat aaggagacaa aagacaataa atacgaccgg
     3601 tataacgaag aaatactaga aaacgaaaca acttttataa aaacacatcc aggcggtctt
     3661 ttgacaatca agtggaaaaa cactggggac aaaactgttc atatccctgt tatcgtctac
     3721 gatcggactg tgctacagca aaatggaaaa acattgactg attatgaagt aacagatatc
     3781 gggaccccaa tcgtgaagca gcaaaaagga atcaacgagc tcacgcttca ttatcaaacg
     3841 ccaatctatt tctatttcat tctttctctt actttgattg gctggtttac cttgctatgt
     3901 ttatttattt atcatagata caagctttta cgtgcttagg caaaagggat aaatacatcc
     3961 gatcaaaaaa ctctccaaac ggtataatcg tttggagagt tttattttaa tcagtttttt
     4021 tatgttatat gttatatttt ttattcgttc acatctaatt cgatttccac cccgtagtga
     4081 tcactgatga ttggactatc gatcccatca aaaatccgtt gatatttttt aacctgcatt
     4141 ccttcaggga cgaaaataaa atctatacgc aatttttctg tattttgttc ccaaccatct
     4201 attttctttt ccacagtagc tgcttttcct ttttcattcg cgacaacata cgaatcttga
     4261 agaagaaggc aactttctct tacagtataa taaccttgag agtctactgg attattgaaa
     4321 tcacctagga aaaaaagtgg tacgcgagtt tccaaaaagt agttttccaa gtttttccac
     4381 tcaaaataaa agccactttt tttatctgtc caccaagaaa agtggcagct cgagacaaaa
     4441 atgtcctgat catctatctt tgttttccca atcaagatct tgcgtgtctg ataatcttct
     4501 ttcttttgaa attgggagac ggtaaggacc tgactttcca atgttttttt cgtaagcagt
     4561 gcatttcctt cttcatattt atcataaccg atgtggctca tttcccaact ccaaaaatac
     4621 tcttttccgt gttcctttaa aagctgaact aaacggtagg caaaattatc tttacggata
     4681 ggtacttgat tctttaccgg acagaacttc attagcttcg caggttctac ttcttcagat
     4741 tccagtaatt ggttgatttc ttgtaaagca atcacttcat aatcttctaa caggatctta
     4801 tctgctaatt cttgcaattt tttctctggt tctggctcaa gccagctatg cgtgttgagt
     4861 gtcatgaatt tcattggttt tctccttgtc ttcatttcga aacaaaaggg ttgtgacaaa
     4921 atttttgtca caacctctcc agcaaatagt gttctcctgc taactcctca gtaataagct
     4981 caatggacca ggagctattt tttgtttggc tgtttttatc acttggtgca gggcagcttt
     5041 ttcacgacct ctccactaaa cagtattcaa actgctgctt ctcggtaata agcccaacca
     5101 aacgaaaaat aggaggagct atttttgttt ggctgttctt atgacttgat gcaggacagc
     5161 tttttcacaa cctctcctta cattttaatg gagccgatca ctttaccagc tgtcactgta
     5221 cctaattgct tgatcactac ttcttctact ttatcgtttg taaatacgat catgatcgtt
     5281 ggatcttttt cttcgttttg gatcacatca agatccatct cagctaattt cgttcctgtt
     5341 cctacttctt ggccttcttt gacagcaatc tcaaaagctg gctgattcat ttggactgta
     5401 tcgattccca tatggatcaa aacttcgata ccatctttcg tttccagccc aattgcatgt
     5461 ttgctaggga aaatacttgt cacttttcca gcgatcggcg caacaacttc tcttgatgtt
     5521 ggaataacag caaagccgtc acccatcatt ttttgtgaga ataccggatc ttttacttca
     5581 gataaaggaa tcacttctcc agaagctgcg gaaactagtt cttgttctat acctaagtat
     5641 tgcttactac ctgcttgtcc atttcctgta gaaaccattt ctggctctgg aatctctgct
     5701 cctgattgca gcagatcttc gatatctgat ttcaagacat ctgcttttgg tccataaact
     5761 gcttggactc cattgtcttt gacgatcaat cccatcgccc ctgctcgttt ccaggcttct
     5821 tcagtcccta ctttttctcg atcatttaca cttacgcgta accgagtcat acaagcatcg
     5881 acatctttga tattttcttt tcctcccagc aaatgaatga tctggaccac ttgctgatct
     5941 gctgttcctg ttccgctgtt agatacaacg gtttcttcac tgtcattgtc gtaatttccg
     6001 ttacgtcccg gtgtagcaaa gttgaatttt ttgatcaaga aatttgctaa gaaatacgta
     6061 acaacaccaa aagcgatcac acacaacacg aagttgataa gatctccacc tagacccgct
     6121 ttgatagcta acggtgtccg agtcagcaat tcgatattcc caaatgaatg gacccgcaat
     6181 ggcaagatat ctgccatcgc aaatgcagcc ccttggatca ctgaataaat gacatataat
     6241 ggcacagcag caaacataaa catgaactcc aatggttctg ttacacctgt tagaaagaca
     6301 gccaacgctg ctgaaaaata cattgattta tatcttgctt tcttatcagg atcgacatta
     6361 cgatacatcg caaaagccat tcccatcaag atacctgaag aaccaatcat ttgtcctact
     6421 ttgaaacgag ccggcgtcca gttatgcaac acaaactcat attgcgacat atcccctgca
     6481 cctttaagat tcactaaatc agtggcccaa gccaaccata acggatcttg cccaaatact
     6541 tgtgtccccg cctgtgcgcc tgataaaatc tcatacgatc cgccaagctg tgtatagttg
     6601 ataggaatcg tcagcatatg atgcaatcca aatggcaata gtaaacgttc taatgttcca
     6661 aataaaaacg gtgctagaat cggtgcgctt tcttgtgatt ctgcaatcca taaaccaaaa
     6721 ttattgattc cagcctgtac gtttggccac accacagcaa gaatcaatgc cacgattgtt
     6781 gaccataaga taacaacaaa cggaacaaag cgctttccat tgaaaaatga caaagcatcc
     6841 ggcaattttc tataattata gtatttatta taagccattg ccccgacaaa tccagcgatg
     6901 atccctacga atactcccat attcaaagct ggtgcttcca atacacttgt aaaaaatcct
     6961 ttaaccatga tcttcgtacc gaacagagtg tgggtaaatg catcttcatt cgcaagcatg
     7021 tcacttgtga caccaaagat agccccagtg atccgattga ttaaaataaa tgagatccca
     7081 gctgcaaatg ctccgcctgc tctttctttt gcccaacttc ctcctattgc taaagcaaat
     7141 aaaatatgaa gattaccaat aattgcccaa ccaatatttt caattactcc gcccgttgtg
     7201 acaagcggcg tccaatcagg attgatcaag ggaattgatt tccctatact gatcatcaac
     7261 ccagctgccg gcataactgc taccacaacc attaaagcct ttccaaattt ttgccaaaat
     7321 tcaaaactaa atatcttttt cattccgttt agctccttta cgcaatcggt ttcgttaaac
     7381 gtattatagc gtgcgttttc ttttttgtaa atgctttatt ttttattttt ctgtctatac
     7441 cattctataa aacctataat acgtgtgttc ccttctataa ttttctccta aaaatttatt
     7501 tttttaagct tttttctatg aaatattttc ttttgtctat tgacttgtgc aatcggttgc
     7561 gtttattatg aacgtataaa acagctcatt aggaggagaa aaatgaaaca gatcaaacga
     7621 ctatttgcta ttgatccatg gaaaatcagc acgacacaat tagataaaaa aaatcttcga
     7681 ctgcaagaat ctttaacaag tattggaaac ggttatatgg ggatgcgcgg gaactttgaa
     7741 gaaacttact caggggacca ccatcaaggt acttatttgg cgggtgtctg gtatcccgat
     7801 aagacacgtg tcggctggtg gaaaaatggt tatccagaat actttggaaa agtgatcaac
     7861 gccttgaact ttatcgcgat agatatttcg atcaacgatc agctgatcga cttggcttct
     7921 ttggaaccag aagactttta ctgggaatta gatatgaaaa atggtgtatt atctcgtaca
     7981 ttcacaatca ctacttctac taataaagta cgcatttcct tcgaacgttt tctgagcatt
     8041 gtcaaaaaag aatcagctta tatctattta actatagaaa tgcttgaagg tagcggaaca
     8101 gtcaaactcg tatcaaaatt ggatggaaat gttcaaaacg aagacagcaa ctatgatgaa
     8161 catttttgga atgagattga tcgtggaact atagaagaaa tcggctattt gacgactaaa
     8221 actattccaa acaactttgg gattgaacaa tttactgtta ctgcagcaat gcgccatttc
     8281 gttaatggaa aatcagtcat ccctgactat cacgaagata ctttagcatt acatggttgc
     8341 ttgtctgcag aattatctca aggtgagaca tggtcagtgg aaaaagaaat tatcgttctt
     8401 actagtagag atattccaga agcgcagcaa gcagaaaaag ctgtctcaga attaacgaac
     8461 cagacaaaca attatatcca agcaaaagaa gagcaaacag cggcttgggc agaacgatgg
     8521 aagcttgccg atgtggtcat cgaaggagac gaagaagcac aacaaggtat tcgattcaat
     8581 ttgttccaac tattctctac ttactacggc gaagatgaac gattgaatat cggccccaaa
     8641 ggattcacag gagaaaaata cggcggagcc acttactggg atacagaagc gtatgctgtc
     8701 cctctttatc ttgctttagc caaacctgaa gtaactaaga atctgctgaa atatcggcat
     8761 aatcaactgc cgcaagcaat ccacaacgct cagcaacaag gattaaaagg cgcgctttat
     8821 cctatggtca ccttcactgg tgtcgaatgc cataacgagt gggaaatcac attcgaggag
     8881 attcaccgaa atggcgcgat tgcctatgca atctacaatt atacgaatta caccggcgat
     8941 acggaatatt tgaaaaatga aggactggaa gttttagcag aaatcgcacg cttctgggca
     9001 gaccgtgtcc atttttcaaa acgagcaaat aaatacatga tccacggtgt gaccggccca
     9061 aacgaatatg agaacaatat caataacaat tggtatacaa atacgatcgc agcttgggtc
     9121 ttgcgttaca cgcgagaaaa ttatttaact tatcaaaaag aagctgctat cactatcagc
     9181 gaagaagaac tagccaaatg gaaagatatc gcagagaaca tgtacttccc agttgacgaa
     9241 gaattaggaa tctttgtcca gcatgacaca ttcttagata aagatctcat gcctgtatcc
     9301 gatattccca caagcgatct tcctctgaat cagcattggt cttgggacaa aatccttaga
     9361 tcttgcttca tcaagcaggc agatgtacta caggggattt atttcttcaa tgatactttt
     9421 acaaaagaag aaaagcaaaa aaactttgat ttctatgaac caatgactgt ccatgagtct
     9481 tccctttcgc caagtatcca ttcgatttta gcagcagaat taggaatgga aagcaaagct
     9541 gtagaaatgt accagcgaac agctcgcttg gatttggata attacaataa cgacacagaa
     9601 gacggcttgc acatcacttc catgactggt agctggttgt cgattgtcca aggatttgct
     9661 caaatgaaaa ctgcccatga tcagttgaga ttcgcaccat ttctgccaaa aacttggaaa
     9721 gcttattctt tccatatcaa ttatcgcgga cgtttattgt tcatctcagt cacagatgaa
     9781 caagttactt tcgagttatt agaaggttct cctctagaaa tttacgtcta tgaacagtct
     9841 tattctttag cggatcgtct agaaataacg ctaagaaagg aggaagcaaa tgtttaaagg
     9901 ggtattgttt gatttagacg gtgtcattac cgatactgct gaattccatt atcgtgcttg
     9961 gcgaaagctt ggtcaggaaa tcggcattac gatcgaccac acattcaacg aacagctaaa
    10021 aggcgtcagc cgagaagact cactctcttt gcttttagct tatggcggaa aagaacactc
    10081 tttttcaaag gaagaatttg cagaattagc gaaaagaaaa aacgattatt atttagagat
    10141 gatccagacg atcgaaccaa aagatgtctt ccccggggtc gtgcctttac tggattcttt
    10201 aaaagaagaa aaaatcaaga ttgctcttgc ttctgcaagt aaaaacgggc cattcctact
    10261 tgaaaaaatg ggtcttacac cttactttga tgctattgcc gacccggcag aagcaacaaa
    10321 cggaaaacct gcgccagata tcttcctcct tgcagcaaaa gctgtcggac taaaagcaga
    10381 agaatgtatc gggatcgaag acgcgcaagc cggtatccaa gccatcctct caagtggcgc
    10441 tcaaccaatt ggcgtgggac gtgctgaaga ccttggagag gagatcccgc ttgtccctga
    10501 tacgactttt cttacgatcg attacttgaa agaaaagtgg catgaccatg gataatccag
    10561 agatctccgt cgtaccagtt ccagagactg aaataaagaa aatcacattg aagaacgact
    10621 acttagaagt cgttcttctc aattacggtg cccgcctgca ccaaatcttc gcaccagaca
    10681 aagaaggaaa atcagaaaat gttttattgt cttacgatcg tttcgaagat gttctaacag
    10741 acaaaagctt ttttggagca actgtcggac ctgtagccgg aaggatccgg gatgcttctt
    10801 ggggtgagca tagattggag aaaaactgcg gatcacacca tatccacggt ggcacgaaag
    10861 gctggtcttt ccaattttgg gaagtagaag ttttcaaaac tccatcatca ataggtgtgg
    10921 tcttttactt aaaagatgaa ttttctacct atccaggacc aatcactgct acgatcactt
    10981 accgtttaac aaaaaacgaa ttagagatga caacctctgc tagtagcctt gttgaaacca
    11041 tctgcaatcc gaccaaccat gcctacttca acctttcagg aaatggaaca agagacatct
    11101 atgaacatca gttaacggtc tttttagatg gtgtgcttga attagatcaa gaaaaactgc
    11161 ctactggaaa ttggacaaaa aaagaaaaac tgccaatcga ctttagaaaa tccccaacac
    11221 tgcaagagat ccttgcatgt tatccagatg gactagatga tgtatttctt ttgcatcatc
    11281 ctaggctttc caaaacttcc ttacaattat tcgagaagca ttctggtcgc caaatgacta
    11341 tagcaacttc taataaaagt atggtattat tttctacgac tggattcgaa gctgatttct
    11401 ctgtcaatgg aaaacagatg cattcaaact acggattagc aatcgaaccg caagaaatcc
    11461 ctgatatcgt tcatttcccc aaatttggat cgatcaacct ccatcctggt caagaacgga
    11521 tcagtcagac gatttatcgg ttctctgcac aataacaaga gaaaggtgtt tttatcatgg
    11581 ctattaccgt aaaagatgtg gcgaaaaaag ccggtgttgc cacctcgacc gtctctcgtg
    11641 tcatcaacga tcatcctagt atttccgaga caacaaagaa gaaagtccga aaaattatgg
    11701 atgaactagg ttatgttcct aacatgactg cccgcaatct aggaaaacgc atttccagtg
    11761 ccatcggcgt aatcttaccc cctcttgatt caaaagagcg gatcggtaac ccattttatc
    11821 ttgagatcat ggaagcaatc aacgaagaag cgcggaatta tgatatgact acagcaatcg
    11881 ctactgctaa aaattttgaa ttgctgttag agaacgtcca gcgtatgcat ttacaaaaac
    11941 aagtagatgg ttttattcta gtgtattcag atagtcaaga tactgtcatt gattatttat
    12001 accaaaacca catccctttc actttgatcg gacagcctta tcatcatgag aacgaaatcg
    12061 tttatgtaga taatgataac cagcttctag gtaaacaagc aacagaattt ttgatccaga
    12121 atggacataa gaatatcctg tttgtgacaa acacgactca tgaaagtttg tatttcgaac
    12181 gatatttcgg ttatcaaaaa gcaatgatga tggctggtct tcctgcgtta tccagtgtag
    12241 tactagagca tccggaggat tatctgtcct ttgagaaaac gttgaaagaa aacgaagcca
    12301 cagcattggt ggtcattgac gatatcttcg cccttcgcac gatgcagttg gcgcaaatgt
    12361 atggctatgt cattccagag acactctccg tcattagctt caacaactcg atcttctcaa
    12421 cattaacaca cccttacctt acaagtatcg atatcgatat ctccgaatta ggacgtgtcg
    12481 ctatgcaaaa gctgcgcgaa ttgatccatg aagaattgag cagtggcgta cgtttggtca
    12541 tccctcatcg cttgatcaaa agagagacag tcgttccttt agaaaaccca tactaaatag
    12601 ctgactcact aaatacgctg accatttatc ctatggtcag ctttttatgt aaaagaagat
    12661 actacttcta acacattgtt tatctgtaaa aaagagcgtg ggacaaaagt aaagaacact
    12721 tttgtctcac gctctaaaca cgtataaacg gcggaagcag aagcaactcc ttcggaaata
    12781 agctgaaatt cacaaaaatt taaaaagcaa ttttcttgag tttcttctta tttcttggag
    12841 ttaaacgctt ctgtcccagc ctcttttttc cttccttcaa ttttttacac gtcgacgatt
    12901 caataaaaaa ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg
    12961 caagtaatgc aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca
    13021 cggtacgaga gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt
    13081 tgataagcca acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag
    13141 atactgttct ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa
    13201 acgagaaggt cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata
    13261 tttgatcagt aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat
    13321 actcatgatc ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac
    13381 ataaccattc atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa
    13441 agaaagaacg agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt
    13501 gattgccccc ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct
    13561 cgctttcatg ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg
    13621 tcctaatatc gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat
    13681 accagcgata tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc
    13741 tgataaaggc gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat
    13801 tgcatcattg aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat
    13861 cgttgtaaat gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg
    13921 taaaaagaaa cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc
    13981 agataaaatc catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat
    14041 ccctggagtg attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa
    14101 tctgccataa aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg
    14161 gaatccgatg atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc
    14221 ttttaaagaa atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca
    14281 tgaaaaaact attcacttgg caggacctcc tactaaatca gcgacaaaac gtcggctgaa
    14341 agacttattt ttttattttt cttctaaact tttttctacc tcaatccccc aagaatcttt
    14401 cttgaagtga ttgatgatca atggaataac gaacatgacc actgtgatag ctgctactaa
    14461 taatgcatca ctcaatgtcg ctgtacctaa tgtagcaaat acgacaacga tgatagagaa
    14521 gatcagcatg cagctgacta cccaagctaa tccgtttccg tttttaccaa ttcggtaagg
    14581 acgttcgaat tctggatgtt tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta
    14641 cacgatacaa taaataacag ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc
    14701 atccattaca ccataaagca aagcaaataa tgaaataacg attgattgtg ttagaacgac
    14761 atttctagat acgccgtatt tgttttcttt atggaatccg aattttggtg gaaggaatcc
    14821 ttcacgagct acttggatga ttgtttttga tggtccagta acccaagctg ataattgtaa
    14881 aagcacacca ataaatacca tgaagctgaa gatatttccg atcacttctg gtaagcctag
    14941 gatttgacag tcaatcaaga taggctgcgt gatatttgct aattccattt ttccatctgg
    15001 tacgacattt gctacaagca tcgcattgat gacattcaat aaaactaatc cgatcaaagc
    15061 gataaatacg ccttttgtat aattttttgt tgcgtctttc aaacgaggca tgtaaacaga
    15121 actcatttcg atcccaacaa aaataaatgt gataccagct aagtatttca atgaatcaat
    15181 atgttccaag tcagggaaag cttttgacca tgaaaagtca cctagatagc cgtttgagac
    15241 tagtccgact ttgaaggctg ccaaaacacc taaaacgaac atgatgacta ctgggatata
    15301 tacccctagc catacaccga tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa
    15361 gatcgtgatg atccagtaga taactaaaat acaacctaaa acgaaccagt gattattgcc
    15421 aagttctaca ttaccaaatg tgtttcctaa caatggtcct aaagtagaag cgaccattac
    15481 catacctggg aacatttgca cccataatag ccatgcaacg acaaatcccc atttgctgcc
    15541 cagtgctgtt tttacccata actgaggacc gccttcttga ggaagcatcg tggaaagctc
    15601 accggaaacc attgatatcg gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat
    15661 caatgtccaa ccgactgcgg ctaacgtagg aatactccga accgttgcac acagtgccat
    15721 tgtcagaccg ataaaggtcc caagacccat tttacttact tcagatttac tcaccgttga
    15781 cactctcctt gattttttgg ataaaataac ggtgtctttg aagtagtttc agcaataaac
    15841 gaaacaattc agcgaatatt tgaggggatt ctttgagttt attgcttgaa gggtttcttc
    15901 aaagacaacc tcaaaacaaa gggatcgaag agcttaggtc tccccttgct cttcagatcc
    15961 aaacatgcgt cagaaacagt tagtattcaa gctgttttct aacatgatta ccttgcttat
    16021 tttgcttcgc ttgccaataa ttggtctgcg tagatttttt ccaatttttc ttgaagagct
    16081 gctttgattt tttcagcata ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt
    16141 acgctggcac gcaatacagt tacttttccg gcacggttcc attcttcatc tgagaatcct
    16201 agttggttca caaattgtaa tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt
    16261 gaagttaaga actcattacc gtaaatgctt cctttcacgt atgaagaata atcatatacg
    16321 tcatggttca atttattcat tgcaactaag tcatcgttgc ctttttcttt gaatacatag
    16381 tctaccatat tgaaatctgg ataagttaat ggatgaactt cgatttcttt gtctcctact
    16441 ttaaatgaca agtcattcaa gaagttgtag aaacgatgtg caccttcgat tgatgcaccc
    16501 attaatttac catatcctgt gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg
    16561 gctgctgttg cccctgcttt tgaaccttcc aagatataag ccccaagtaa tgctgggata
    16621 tctgcgcctt tttcaaatac atatgtcgca aagtaagaga taacgtctct catgcggata
    16681 tctttgatga cgataccacc ggcagaatat ggaacatagc ccattttatg tgggtcaatc
    16741 gttacagatt ctgcttcttc gattgcttta tatgcactgt gtacttcttc taagatataa
    16801 tctttgtttt ctgtaaagac attgtatttg taatgaacat cttttaaatc ttcaaatgga
    16861 atgaagttgt tgtcttcgtc taagaaaatc gcacggccgt atccgccata agcagcatct
    16921 acgtgtaagt agaagtaaat accgtctttt tctaatacgc gacgcaatgc aacgatttta
    16981 tcgataccgt cgattgctcc ttcttctgtt gatcctacaa caccgactac acctaggatt
    17041 ggtgtttttt cagctgctag accacgaacg attttttcaa gttcgttgat gtccatgcga
    17101 tagttgtggt caactggcac tggaatcact tggtctaaac cgataccgat gatgtcggca
    17161 gcttttagcc atgaatagtg tttagtttgt ggaaccaacc attttcctaa tttttctaaa
    17221 tgttttccgc tacgtgctga gtgagctttg atttcgtcga ttttttctgg gacgctgtct
    17281 aacagattca tgatttcttc tgttgacaag ttcattaatt cccaatcgct ttttccagca
    17341 actagttcag gtgtcacttc tttcattgca agcggcaatg atttgatgtt gcgggcatac
    17401 caaagtcctt ctaagtttgc taaagaacca tcagcaacga tgtggcccca gccatcttta
    17461 tagctcatta attttgcaaa ttccattcct acttcttctt ccatttggct tgttgcagga
    17521 gaagattcat aggcaacgtt attaccattc catagcatag caaaattgta cgctagtaaa
    17581 gatggcatca atgtttcaga gttcatatga ccccagtaac gtccggcatt atgccaagga
    17641 acggaatggg tacgcatccg cgctgaaatt tctgatagaa catcctttgt tttattcaca
    17701 gtgtgttcaa aactagcact gcttttttct tctggcgtaa taataggcat gtcttgaggc
    17761 atgtagtttt gacgccagcc taaatgttca tccaccaatt cattcaacaa agctttatag
    17821 atttggccgt tctcagcttt gtccccaatg aatagggcat ttaggtttag atctttcgac
    17881 aatgattcac tcatagtgaa accctcctaa aaaaatattt aaattttgtc gagccctaca
    17941 ttacacctgt tcttctattc tgtaaactaa gttttggcct gatttttaat catattttaa
    18001 tatgtatcaa ttatccgaat tgttcacact tttgaaataa ttaaaaaaaa cgagggaatt
    18061 tacaaatgta aactccctcg caaagattgt attaacgaca accagcgttc tattttagga
    18121 tgggtttcaa aattggaatg ttgtaatagt atttcccttt ttcaaggtaa aaacttacta
    18181 aattctaatc aattactttg gccaaaaagt aatttttctt accttttcga ataaccacaa
    18241 acttaccatc gaattcatcg gatggattta tgacaaaatt taaatcggta acgcggtcac
    18301 cgttaatact tatagctccg tttgaaacat cttcacgtgc ttgacgttta gatggttcaa
    18361 tttttgtgga aacaagcagt tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg
    18421 gcattttacc gaatccttgc gcgatttctt ccgcatttag ttctttaata tttccagaga
    18481 acaatgcttg cgttattttt tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg
    18541 taacttcttc tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc tctacttttt
    18601 tcgctaagtc ttcgatttct tcttgagaca agaaagtaaa gaatttcaag tatttcacta
    18661 catcgcgatc atcttgattc aaccagaatt ggtagaactc aaatggtgaa gttttcttcg
    18721 gatcaagcca aacggcaccg cctgcagttt ttccgaattt cgttccgtca gccttcagca
    18781 tcaatgggat cgttaaaccg aacgcttctg cttctggtcc ttctaatttt cggattaggt
    18841 ctaatccagc tgtgatattt ccccattggt ctgctccacc gatttgcaat tgcacatcgt
    18901 atgttttatg cagatgcaaa aagtcgatag attgcaggat ttggtaagta aactctgtaa
    18961 atgagattcc tacttccaaa cggctggcaa cgatatcttt tgctaacatc gtgttgatgt
    19021 tgaagttttt accgtagtct ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt
    19081 agttgttcac aaatgtgata tttgcatctt taccaaacaa ttttctcatt tgattggaaa
    19141 gtgcgtccac gttatgttgc acttgttcca tcgtttgcaa cacacgttct gttttgcggc
    19201 cgcttgggtc gccgatcgtt cctgttccgc cgccaatcag aatatatgga tgatggcctg
    19261 ccaattggaa tcttttcatc atcataaatg gaatcaaatg tccgatatgc atcgaatcac
    19321 cagttggatc aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc
    19381 tttcttcgtt tgtttgctga ttaatggcgt cgcgccaagt taattcatca ataatattca
    19441 ttttactgac ctccgtgtag tttttttatg aaagcaggaa cctgctagca tgtgtaatta
    19501 attatttctt caggatagga taggaaataa aaaaagtccc ttggcatata agccaaggga
    19561 cgaaatctcg cggtaccacc caatttgtat gtctagatat ccaacatacc tctcgaacat
    19621 ttaacgctgt cgcgtggcaa tcactgccaa agactcctga aatgtaattc gctttaaagc
    19681 atgtactggt ttgcagcgac caccagcttt ctgaaacatt ggctttaaca actacttcgt
    19741 ttcgatcttc gtcttgttat tattaatatg tgtctatcat agagcgcaac tttttctttg
    19801 tcaacagaaa aaggatattt tttttaaatt cctcattttt tcttttttga atccgctcta
    19861 attccgaaca ttttattttc aattattgtt aaaagaaata actttttctg tattttttct
    19921 gcaatttatc gagaagtcac tttactttgc ctcttattct ttcttcgtta caataaggaa
    19981 tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat
    20041 tattttagga gccacttctg ctttaactat cgttatttta attctaagtt tgttttttgt
    20101 catccgatca tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc
    20161 aaaagaaccg acagatgata agcaacaact agttactcaa gcaaaaaatc tggctcaaag
    20221 ctacgattat gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg gatctactga
    20281 aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg actggtctga
    20341 tcctactcag atctctcatg tctttttcca cagcttgatc gtggacccgc aaaaagcctt
    20401 ccactctcaa caggcacaag gatacaaaga ttatatggtg actgttgaag aattcaaccg
    20461 ttcgatcgaa caactttatc aaaatgattt tgttttagtt aatttgaatg atttgatcca
    20521 aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg ctacctgaag gaaaaaaacc
    20581 attggttctt tcacaagatg atgtcagcta ttatgaatat atggataatt ccggttttcc
    20641 tagccgattg gtcgtatcaa ataacaatga aatcaaaaat gtctataaag agaaagataa
    20701 agaagaaatc ggtgactttg acatggttcc tctgatcgat gcctttgtca agaaacatcc
    20761 tgatttttct tatcgaggcg caaaaggtac cttggccctg acaggttaca acggagtttt
    20821 aggttatcga acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa
    20881 agaagctaaa aaagtggcgg aacagttgaa gaaagacggc tggagctttg cttcccatac
    20941 atggggtcac ttgaatatga ctcaagcttc actagaagat gtaaaaaaag acaatgaatt
    21001 gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat attctgattt atccatttgg
    21061 tgcagacatc agcgactggc agccttattc agcagaaaat caaaaatttg cttacttgaa
    21121 gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct acaccagctt ggggacaatt
    21181 aggtacggat ttctatcgaa atgctcggat caatatcgat ggcattcgtt ttcaatctga
    21241 tttagacgga aaaaatccta ttcttgatcc attcatcaat gtccatgatg tctatgacaa
    21301 agaagcacgc gaatctgctt gattttacgt catttatgaa ccaaaaaagc cgaactgaca
    21361 ttttatcgtc agtttcggct tttttatgct ttatttcttc ttcggtgcga ttcccttgat
    21421 caggacttct gtccattctg ctgtatatgg aagcagtttt gcgtatatct ccggcttaga
    21481 aattccttct accattgctt gaaaatagag gatcaagaac tcatccgaat atttcaaatc
    21541 cacttttcct tctttccgcc ctcgatggaa aagatcaagc atgatactga aactttcttg
    21601 cgtatacgca tccatcgcgt cagaaaattc ttttttttct ttttttgtat agtatgtcat
    21661 caaatcgata taaaattgtt tgttcactcg attgagataa cggattttgt tttcactcat
    21721 ggcaatcagt gtttcttcaa atggaagatt ttccgcaatc aacgctttcg cttcttgccc
    21781 taaatcattc ataaatgcaa aaaatacttc ataaatcagc ccttccttgc tgccaaaata
    21841 tttaaaaatc gtcgctttac ttacctgagc gttctcagct accttatcca ttgtcactcc
    21901 agcggttcct atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct ctttcttttt
    21961 ctcttgtgct ctcttttcaa aaccgttcat ttgtagagcc tcccgtaatt ttaaactttt
    22021 ataatatata tagttcaaat ttattctgag gtttattata actatctatt gagaaagtat
    22081 caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa gaatattatg
    22141 tactaaaata ataaaaaaag ttttataaaa aggtataaaa cctttttatt aaactattta
    22201 atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa
    22261 agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg tctctttcac
    22321 cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa aatccacgac
    22381 gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga tttttgggca
    22441 agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc caggcgatat
    22501 ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga agcttcatgg
    22561 aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg atcctaagaa
    22621 aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc
    22681 tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc ctttgatgga
    22741 aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc
    22801 ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca tacgcgaagg
    22861 aacactcgat gaagaaggaa cactcgatga attgcgtcat ttgactcgtt cgaccatcac
    22921 tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc atgactttgt
    22981 gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg tgattttagc
    23041 tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt
    23101 attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat tttcacgctg
    23161 gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga tatggctagt
    23221 cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga cttccggaca
    23281 aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa tggtcggacc
    23341 aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt
    23401 gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga gccaaacgag
    23461 aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg gacgtcaagc
    23521 aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag caatatcgat
    23581 cagctgtacg atgatttttt tccaagcgga ttcgatcact gcagaaggtg cttggctttt
    23641 tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt ttgctgcaca
    23701 actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag gtggacttta
    23761 ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga atcctttagg
    23821 ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc tttatttgtt
    23881 tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc gagatttgaa
    23941 caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc ttttatcctt
    24001 gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta tcacttttgg
    24061 tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt tagaaagtaa
    24121 cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa actttacaag
    24181 cacgatcatg gtcgtcttga tttgtctagc tgctatttta ccaattgcta gcatcaatcg
    24241 tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa aaatttctag
    24301 gaaccaactt tattggacca atatgttact agctattcta tttgctatat tggcaatcct
    24361 gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg cgaaagacat
    24421 ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt tagtgtttat
    24481 cggaattagt ggctttctat tcggatggct tcctcgaatc ggtaaacttg cttatatcta
    24541 cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac cagaatggtt
    24601 ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt ttgatctttc
    24661 cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt ctattggtta
    24721 taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca tgctagcctc
    24781 caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa gtcttctaac
    24841 tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc attctcctct
    24901 aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc agcaagttct
    24961 ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa gtcaagcaga
    25021 tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg ctgctgtact
    25081 tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg ataatgcagt
    25141 aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc tagtgcttcg
    25201 ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata tccgcccggc
    25261 tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac tttcatactg
    25321 ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg ttctcctttt
    25381 tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg tactaagtga
    25441 ttgaacaaag gaacaaagga acaaatgaaa cacataaatg atcatgactc ataatctctc
    25501 ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc aactgataat
    25561 tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag agtggatttc
    25621 tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat tcctatcaat
    25681 tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa gccgagagta
    25741 aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc gcacctagct
    25801 tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca acgtgttcga
    25861 tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc atcgcttttt
    25921 tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag tggtcgtcaa
    25981 gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt ttgctccact
    26041 ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt tcttcgcttt
    26101 tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa actgctagac
    26161 aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat
    26221 tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata tcaggcaaaa
    26281 gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta tattctttct
    26341 tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat gcgtagattg
    26401 gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct ctttccgtta
    26461 tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga aaaagctgtt
    26521 ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac cgcagtttta
    26581 ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg cactagaagc
    26641 agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc gtcctttttt
    26701 agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg ttaaattccc
    26761 tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat cgtcagcttc
    26821 tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc ctaatttttc
    26881 ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga atgcagatcc
    26941 gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga atactttgaa
    27001 tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag ctgaaacccc
    27061 tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat cactgagata
    27121 ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac
    27181 ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa tcgtctgtac
    27241 attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag
    27301 accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta cattgacgcc
    27361 tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca tcaaaggcgt
    27421 cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt aatcatctaa
    27481 aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt ctcgacgata
    27541 gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta gttcgaatcc
    27601 tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg tcttagttgc
    27661 ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc
    27721 atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc cagcagtata
    27781 cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt caatatcgtc
    27841 catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat aattcttaac
    27901 gatcagcaaa actccttttc cactatcggc ttccttaata gccgtctgaa tctgatctgg
    27961 agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt cccctacaaa
    28021 cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa cttgttccgc
    28081 tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt ctggatagct
    28141 ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca tgattttttt
    28201 caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt tgctgagtat
    28261 catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa ggcgcatcca
    28321 ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt tccgagttca
    28381 ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt gattcattga
    28441 ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg ccgagagaaa
    28501 aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata ccatctgtta
    28561 ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc ctttctttta
    28621 catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat ataataaatt
    28681 tctaattttg ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga
    28741 gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc
    28801 ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga
    28861 ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg
    28921 tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc
    28981 gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt
    29041 cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaataacta
    29101 aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta acttcgaggt
    29161 actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg taagctgctg
    29221 cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt tcatttgttt
    29281 ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact gtttctgtcg
    29341 tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt attcgttgga
    29401 aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt tgtgcttcgc
    29461 tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt tgacttagct
    29521 ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt gcttgttgga
    29581 tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg gcaagctttt
    29641 tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa tcttgatcga
    29701 ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct tcgagttctt
    29761 ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta gaactattta
    29821 ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt acattggaag
    29881 tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatggtt gttgaaggtt
    29941 ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag aaaatactgg
    30001 aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt cccatcctta
    30061 ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca taaacacaac
    30121 aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg ggcttttcca
    30181 tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg tcacgatcgt
    30241 tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa ggtatttcaa
    30301 ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc ctgcgaccat
    30361 ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt cgtagtttcc
    30421 ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac aaaagaatat
    30481 tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa tatgtttaaa
    30541 aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac cttttgccca
    30601 acgttttctg tgtcgatata gcatcttcaa cgtcacaggt acttccatga aaaagataat
    30661 ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa tactgatgtc
    30721 ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt ttcgatacat
    30781 cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg ttcgtttaat
    30841 gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt tggtacgatt
    30901 ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc gaatgaaata
    30961 attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat tgcttaaaat
    31021 caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc ctttattttt
    31081 ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta atatttctgg
    31141 tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat aattcaattt
    31201 cgtcatcaaa tactcgattg tatcttcgat cacaatttct tcattatggg ctggcaccat
    31261 gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt gcttatgttt
    31321 gtataaaaac acataacacc agacaccaat aaaccataca aatccgccta aaatcggata
    31381 agaaacgaga aaaaaataca taatagacaa acaagtatct aagaataaat tacctgtatg
    31441 ggtgattgag atcatttcag ttccacccca ttttctttat acaaatctgc aatgaatgtc
    31501 gtatctagat ttttctcttc ggagacagaa taaaaacgga ccgtttctct ctcttctttt
    31561 gttccaaaac gttccgtata cacttcttca agtaattctt gtcttttttt caatttttca
    31621 tcttcat
//
LOCUS       DACCQI010000015        31360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCQI010000015 DACCQI010000000
VERSION     DACCQI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.015"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            4..243
                     /locus_tag="IVO76_000562"
     CDS             4..243
                     /locus_tag="IVO76_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428551.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            310..549
                     /locus_tag="IVO76_000563"
     CDS             310..549
                     /locus_tag="IVO76_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428552.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            635..1306
                     /locus_tag="IVO76_000564"
     CDS             635..1306
                     /locus_tag="IVO76_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7428553.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1359..3335
                     /locus_tag="IVO76_000565"
     CDS             1359..3335
                     /locus_tag="IVO76_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7428554.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3348..4103
                     /locus_tag="IVO76_000566"
     CDS             3348..4103
                     /locus_tag="IVO76_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7428555.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4100..4924
                     /locus_tag="IVO76_000567"
     CDS             4100..4924
                     /locus_tag="IVO76_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428556.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5213..7324
                     /locus_tag="IVO76_000568"
     CDS             5213..7324
                     /locus_tag="IVO76_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7428557.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(7362..9419)
                     /locus_tag="IVO76_000569"
     CDS             complement(7362..9419)
                     /locus_tag="IVO76_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7428558.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(9533..9745)
                     /locus_tag="IVO76_000570"
     CDS             complement(9533..9745)
                     /locus_tag="IVO76_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428559.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            9932..10399
                     /locus_tag="IVO76_000571"
     CDS             9932..10399
                     /locus_tag="IVO76_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428560.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10396..10638
                     /locus_tag="IVO76_000572"
     CDS             10396..10638
                     /locus_tag="IVO76_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428561.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            10656..10958
                     /locus_tag="IVO76_000573"
     CDS             10656..10958
                     /locus_tag="IVO76_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428562.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11030..13084
                     /locus_tag="IVO76_000574"
     CDS             11030..13084
                     /locus_tag="IVO76_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428563.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            13084..15696
                     /locus_tag="IVO76_000575"
     CDS             13084..15696
                     /locus_tag="IVO76_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7428564.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            15693..18224
                     /locus_tag="IVO76_000576"
     CDS             15693..18224
                     /locus_tag="IVO76_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428565.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18276..18608
                     /locus_tag="IVO76_000577"
     CDS             18276..18608
                     /locus_tag="IVO76_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428566.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            18609..19202
                     /locus_tag="IVO76_000578"
     CDS             18609..19202
                     /locus_tag="IVO76_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428567.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19365..21059
                     /locus_tag="IVO76_000579"
     CDS             19365..21059
                     /locus_tag="IVO76_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7428568.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            21747..23624
                     /gene="ltrA"
                     /locus_tag="IVO76_000580"
     CDS             21747..23624
                     /gene="ltrA"
                     /locus_tag="IVO76_000580"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7428569.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            23755..24132
                     /locus_tag="IVO76_000581"
                     /pseudo
     CDS             23755..24132
                     /locus_tag="IVO76_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24132..25337
                     /locus_tag="IVO76_000582"
     CDS             24132..25337
                     /locus_tag="IVO76_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7428570.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25353..25997
                     /locus_tag="IVO76_000583"
     CDS             25353..25997
                     /locus_tag="IVO76_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428571.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            26008..26814
                     /locus_tag="IVO76_000584"
     CDS             26008..26814
                     /locus_tag="IVO76_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428572.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQIESFQATIDLNEELVKKLKDQLP
                     YQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQKLQEKIDRMTKENI"
     gene            26837..27066
                     /locus_tag="IVO76_000585"
                     /pseudo
     CDS             26837..27066
                     /locus_tag="IVO76_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27225..28160
                     /locus_tag="IVO76_000586"
     CDS             27225..28160
                     /locus_tag="IVO76_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP7428573.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCIMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            28204..28473
                     /locus_tag="IVO76_000587"
     CDS             28204..28473
                     /locus_tag="IVO76_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428574.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            28705..29001
                     /locus_tag="IVO76_000588"
     CDS             28705..29001
                     /locus_tag="IVO76_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428575.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            29003..29425
                     /locus_tag="IVO76_000589"
     CDS             29003..29425
                     /locus_tag="IVO76_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428576.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            29444..31036
                     /locus_tag="IVO76_000590"
     CDS             29444..31036
                     /locus_tag="IVO76_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428577.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            31057..>31360
                     /locus_tag="IVO76_000591"
     CDS             31057..>31360
                     /locus_tag="IVO76_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428578.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT        11444 a         4846 c         6403 g         8667 t
ORIGIN      
        1 tagatggcgc gaccagtttt agaggcgatg gatatggata ctttgacaaa aggaaggtat
       61 gtacagctgt tagctcgtgg ttattcacct aaagaagtat tcaaagcctt atctaaagga
      121 acagatatgg aaaaagaaag attgcgaaaa gaattcgatt attggagaac ccataatgga
      181 atcaaggatt tgaaaccagc acggccagta attaaacgaa agaaaaagat aacccaagac
      241 tgaagtgtag agcgtatgtg atcataggct tttttatttt tgcaaaaaac aaggaaggta
      301 ggaaaactca tgaattatga agaaatcgaa aacagaaaaa aagtgtcgaa agaaatggaa
      361 gaaaagttac taaaaacgat gaagcaaaaa caccttaaaa gattatctgt cgcgcaatat
      421 atcaatgata tgcagttaac aggaaaagag aaagcttgtt tgttaggttc aatgaaaaac
      481 tttgaacagt tgagacgaac ttatgtgaaa aaaagttcta attgtcaact attgttagaa
      541 gtctcatgac attcattaat agaattcatt tttaaaactt tttctatgga aatgagaaaa
      601 agattttttg ttttgtctga aaggaggtag ctggatgaga aaatggttat ttggtttttt
      661 aggagtcgct ttaatcgtct tttgcagtgt gtttggctat gttagctacc aaaaacatga
      721 aggagaggtg tttaagcaaa atatagaaaa aaagatgccc gtagatcaaa tcaatgcaca
      781 tgcaaagagt tataaggagg atgcaacgaa cgtcaataat gatatgtctt tggggcaaat
      841 gttgtccata caaaaagaag caatcgaaat gggcgtcaat aaacaagtat ttgcacaaat
      901 tcagattcct gcacttggtt tagcattgcc aatttttaaa ggtgcaaatc aatacacttt
      961 gagtcttgga gcggctactt atttttatga agatgctgaa atgggcaaag gaaactatgt
     1021 cctagcaggt cataacatgg aaatgccagg tgttttattt tctgatattc aaaagttgtc
     1081 attaggggag gtgatggact tagttagtaa cgatggcgtc tatcgttaca aggtaacgcg
     1141 taaatttatc gtacctgggt actttaaatt aatcgatggc gttcctgaag agaactcttt
     1201 cttgtctttg ccaaagaaag gagagaaacc cttattaacg ttgtttactt gtgtttatac
     1261 gtcacaagga aaagaaagat acgtggtaca gggagagcta caataacaag gttttaatca
     1321 acgcgagtcg ttgaaatatg aaatgaggag agtaaaaaat ggcaataaat cggtatgtaa
     1381 aaaaagctgg aatgcttgtt ggaatgttag gaatcattgc aggctgcttt caggggtatc
     1441 aagtacatgc agaagatgtc acgcaaaaaa caccaccaga gaaggtcaat atcacggttc
     1501 ataaattaat gtacgatcaa ggaacgcaat tgaatgttga tattgacggg atcaaaaacg
     1561 atggttatac gcacgatcaa tatcctacag gtgtaacgaa atacaacaag gcagattatg
     1621 gtgatgttga gtttacgtta ggaaatatta cggatcaagt attaccgaga gaagattctg
     1681 atctaaccaa cgcgaaagtg gacgagattg tacaagatgt agaagacaag ggatctaaca
     1741 gtgagtacgt caagaatgca accaacaaaa ttacttcagc tgtagatgaa aatggtgaga
     1801 tcacttttac tgatcaacca gcctatgtca attccaaagg gaatgtatat gtggtttatg
     1861 aaagtaaatc cgctgctgga ttagtcacac aaaaagcaaa accaatggtt gtgattgcgc
     1921 caatgacaga taaaacagga tcaggatttt taaaaaatat tcatttgtat cctaagaaca
     1981 ttgtttctaa gttgagtttt gaattaacga aatttggtga cgatggcgca gaccaaagca
     2041 aacaaaccct tttaaaaggg gcaaaatttg agttatacaa gggcgaacct ggaaaaggaa
     2101 caaaattggg tgatcttgta tcggatgatc aaggaaagct gactgcgact gatttgacgt
     2161 taggaaaata ctattttgtg gaagttcctt cggaagttgt agtgggatca gatcaagaac
     2221 caacagcaga tcaatattta ttaggcgcag acgctcgcaa cgatgcgcac aataaattaa
     2281 cctttgaaat tacgaatgat ggcgtgacaa gtgacttaaa agcttcatac gtaaactata
     2341 aagcaccagt gatcgataaa acagtaacaa atggaacagg acaagaacat tctttccaaa
     2401 ttggtgacgc agtaaattat caaggaacga ttcatattcc aacagacatt gcaggtggcg
     2461 cagacggtat cacagtaaac ggtgtgaaaa gcgaaacttc tccttacagt gtatttaaat
     2521 ggggagatac agcaggtaaa ggactttctt atgttgctga aaaagcagat attaaagtaa
     2581 caaataatga tggttcagtc gtattaaaag aaaatactga ctataaaatc gaaaattctg
     2641 atagtggttt tgtgatcgat tttatcgtaa ataatggaca agtaagtgat acagttgcga
     2701 atttacatgg tcaagatcta aaaatgactt ataacatgtt tgtgaatgat agtgctgaaa
     2761 tcgcaaatcc attaacaaat agcgtggatt tcgtttataa caataatcca tttaatcagg
     2821 aagaacacca cgaaaaaacg aaagctgacg ttgtgactta tggcgcaaaa ttcttaaaag
     2881 tcgatagcgg cctatttgga acaggtatca aagcaacacc gcttgaaggc gcagaatttg
     2941 cagctaagaa tgcagaaggt aagtactacg gtggacttac tgatacagat aaagatggtg
     3001 tcaaagaagc agtttgggta gacgatgtag caaatgcagc aatcctaaaa tcagataaag
     3061 agggtcattt tgaaatcact ggtttaactg aaggagatta ctcactggaa gaaacaaaag
     3121 ctcctgaaaa ctatcaaaaa ttaacaaaag agatctcttt caaagtagac aaagattcat
     3181 acaaagaaga aaaccgaatc acgatcaaaa ataatcaaaa agcttccgta ccaatgactg
     3241 gtagcaacgg tttccaatct tacgtattga tctcttgctt gttgctagga gcagggggac
     3301 tttctgcagt tgtttacttt aagaagaaag cctagaagga tcagctaatg attattcgta
     3361 agcccccaaa aagaagagtc atgcacaaac tgatgatatt tttttggttg ctgtcggcag
     3421 tagcagtcta tttaattcca gttgtggaaa caaatgtagc gaaatttcaa caacagcaaa
     3481 gaattttagc cattgaaaat cttgaaaaaa cagattcttc tttggatgtt gcgaaaatag
     3541 agttaggcga tcctgttggt gttcttacaa ttccaagtat ttctttaaaa ctaccaattt
     3601 atgacggaac ttccgacaaa attttagaga atggtgtagg gattacggaa ggaactggcg
     3661 atattacagg aggaaacggt aaaaatcctt taatcgcggg gcacagtgga ctttataaag
     3721 ataatctttt cgatgatttg ccttcagtaa aaaaagggca aaaattcttt attaaggtaa
     3781 atgaagaaca acatgcctat caaatttatc gaattgaaga agttaaagca gaagaattac
     3841 aaaaaaatta tttagattat cttatgcctg aaaaagacca agataggatc acgttaatga
     3901 catgcacacc aaaaggtgtc aactcacacc gctttttagt gtatggtgaa cgagtttcct
     3961 tcagtaaaga agatttaaaa ctacagaaaa aagcaaaaaa taggaaatca atttcattgg
     4021 attggcgatt agcactgcta ttcgcttttt tttgcgttcc tattattttt atttataaaa
     4081 aaataaggag gagatcggaa tgaataagag aaaatggctg tttgtttgtg tagcagctct
     4141 cttttctact gtagcttatt cgcaactgtc tttcgctgac agcagtgata gtagtaccac
     4201 tcaaacaata gataattcaa ttactgcacc aagcgaaacg gacagttcgg agaatactac
     4261 agaagaaacg gcagattcaa atataagctt tactgaatca acgaatccaa cgtcagaaag
     4321 ttcttcagag acttcagaaa agccgcaaga cgtagaagat acgcaagtga tcaagcaagc
     4381 cattcatatt caaaaaatta tttcttcgaa tcaaatcaac aatcaaggca gtcaagtagc
     4441 ccagcaagaa ggtatcaatg gcgcgaagtt tattgtctac gatatcacga atatcttaga
     4501 agaaatgtca caagatgata atgagaaaaa tcaatctgta gaaataattg agtcaaaatt
     4561 gaaagatcga gcaaaaaaac tttcatacga ccagttgaaa aaagtggctg aaggagaaac
     4621 caaaacgatt gatgatcagg aaggcgtgat tgaattttcc attgaagtac ctgcaaacaa
     4681 aaaacaagca tactatatcg ttaatgaatc aagccctgaa aacatttcaa acagtgaaga
     4741 tattgtttta ttgacacctg tttcaaatga aaaaggtgaa tttttgacgg atgtttggtt
     4801 ataccctaaa tcagagaaat cagagccgaa agaagaaatt aaaaaagttg tgtcaactgg
     4861 tgtaaagaag aacttttttg aaaattgttg ggaatttttt gctcaattat tttttgataa
     4921 ttaggatgaa atgatgaaaa taataagaat attagttttt aatctatcct ttttagccgc
     4981 aacaatattg tgttgcggtt taattttttc tcaattatca atttatccaa gatatcaacg
     5041 attgttcatt cattttctaa acattcaatg tgcttccatg ccattagagg tgattaaaaa
     5101 gctcttgatc aatccaattt ttatggttag tttggttctt cttacaggat tggtaacgtg
     5161 ggtattagga aaaaaagaat gaaaagctta cgatcgttta aaaaaatatt agatggaggg
     5221 cattatggaa aataaaattc attgcactac gggaagagta attactttag tgttgttttt
     5281 gttccttctt ctttttgcga ggggaactgc agtccaggcc ttagaaaaag ttgatccgag
     5341 tcaaatcaaa gaaaataata atctggtgct ttatcatgat agtgattcat tgagaaatat
     5401 cgaagtaact gggatcaatg gatttaagat actagatgag tattcaacta aaaaacaaaa
     5461 agtacttgct tggccaaaag tcaaagattt gaaaagtacg tcaaaaatcg ttgcccatta
     5521 tcaaaaagta ggtacttatc taggaaaaga aattgattgt gacgtgactt tttctaattt
     5581 tgtagcgcac gctggggtta accaagacgg attagctaaa acaattaatg gcaaaaagta
     5641 ttataattat ttgctctttg ataaaaatat ttatcgagga tttttcatgc agtcaatcaa
     5701 tgtcatgaat gcaaaactag aatttaagta tgcaggaaca aaaacaaaag tagctttgag
     5761 tgaaccacag ccagaaattc aatcaaaaat gagatcgatg agagcagctg gtattgattt
     5821 aaacgataca tttctatcca tttacagttt aaatggacag tatcagcaac gtgtgtcttg
     5881 gtataatgac aataatgaat ctgtcggtga acgtaatatt gatatgagag aatttgttgg
     5941 gtatgaaaaa atgggtagct taccttattt tgtcacaaca gatacagcat gtgcagaata
     6001 caaagctcct gcgttatcaa caaacaattt aacttcaaaa gtagtgggag gacgtgcaga
     6061 aaaggcttat agctcgaatg atcatttcac cgatgttgta ggagctgata cttatcacag
     6121 aagtggtgta acgtatacgc ttcaaggcgc ttccccaaca ttcatgattg gcgcaaatac
     6181 gaatagtatg atgtttagct ttgatactgc attgctatgg acaccacaac catcgaagcc
     6241 tacaaaagaa gtgtttaaca aagctaatac tgaagaggca gcacacaata ttgacaaaaa
     6301 agtgattcca caaggatcag atgtttacta tcatattcac caaaagtttg atgcattaac
     6361 agtcaacaca atgaacaaat acaaatcatt taaaatcact gatacctttg acagcaaaaa
     6421 ttttgatatg gtatcggatg ggaaaaacta tgatggcgca ttgtggtatg ttgaagcgaa
     6481 tggtacttgg aaaaaagctg atacaggagg aaagttaact gtatcaggta atacagtcac
     6541 ttatactgct tctgacagct ttttagctaa cgctgcaaat tacggtaggg attatatttt
     6601 gcagctccat atgagagcaa aaaatgatgc aaaaggaatt gcaccaaatg tggctactgt
     6661 aacgtttaac aatgatccga acaaaggaac acaaaaaaca aatacagtag aaaactattt
     6721 ctctcttaaa ccaaataaaa aagtggaaca agatgggaaa gatgttgatg gtcgaaatga
     6781 tggtaaaaaa ggaacgccta cagctccttt aaatgcagga tctgaagtac aatatctagt
     6841 cacacaaaaa tggcatacaa aaggtgtaga cattgcctca gatcattaca agcaatttag
     6901 tattcaagat ccagtggaag atcgtttgac gtataaagaa ggttctgcgc gagtgataga
     6961 tatgagtaca ggaaaagata ttacttcaga aggtacacta acctatgata gcaattcaag
     7021 aacgctgaag tgggaagctt ccgctgattt cttaagtaaa aatcttttag atggacgaga
     7081 aattcagctg atatttacag ctaaaactcc tttgaatgaa gaattggata tcgacaacca
     7141 agcaaaagtt tcagtagaaa gtattgagaa agcaactaat gtagtaacga ttggggtaaa
     7201 acctaactta cctcagatca ttgtgccaaa aactggatcg aatcatttag tgctaactgt
     7261 tattggaagt atattatttc ttactttagc atcattgggc tatgtagttt taaaactcaa
     7321 ataaatataa aaacaaggct gagaatcagc cttgttttct tttatttgaa gattggattc
     7381 gcactaataa atacattatc tacgccaatt ctaccagcag agtcaactac aaaattatga
     7441 cctaatccga gaatactttg gttattataa agtaaatttg cattatgggc agggctattt
     7501 ttccatccta ccatggcatc gatagcacca tcgtctgata aaaggtcatc aatatacagc
     7561 ccagtttgac ttatgttttc tcccttagca ccgtagcagc ctaagctttc caataaatct
     7621 tggaaagtac caccaccagg gcgatagtga ctgaagctga cttgtaattc tttagctcga
     7681 atatctgcag cctgttgaac aatatcattg aattgcattg gggcaacacc attttgttgg
     7741 cgataatcat ttaaccaagc aagttctttt tgacgcataa agttacgtaa ttcgtctaat
     7801 gaaatctttt tatatttgaa tgtgattgtt ttattttgat cgacgttatt aatggatcgt
     7861 tggccgtttt tatccacggt aacggtagga tcgtcatttc tgcacaagaa ataattttga
     7921 tcagtcatgt ttttccaagg agtagcaaag ctatcccctt cgtatactgt ttttgattca
     7981 ctagagagaa cagcgttggt atcagcattt acatgattaa cagtcacatt aaatttacga
     8041 gtgtagttga aaatataatt ttgactttca gtcacattgt ttaaggttac tgaatttgtt
     8101 ggttctaccc gatcgtctaa aacatatcct tgaatagaag gagcagttaa agtgacactg
     8161 tcaccgtgag tgacattgac tatctgttct ttcaaaatat taactcgtcc ttcgtttttg
     8221 tagttgttat tgcacaacaa attgatttga tgcacttgat tataattgaa tgtgattgtt
     8281 gtattacctg tgattgtttt gctttgagta tcattaccaa tcactttgta gctaaaatta
     8341 tctttagcgc cttcgctata tttaaatgct tttgccttag cagtgtaagt agaaccagaa
     8401 gttacttttg cttgatctgt agcaatcact tgacctgttt gattgtcaat atggttaata
     8461 gtaaccgtat attcttttgg tgtttcaggt ttgtgttgca accctttaaa tgcagcatca
     8521 agactatttt tagcattgtt tacttgtgtt tgagaaacct tagaatcgtc tagtactttt
     8581 ttagcattgg ataaagcagt ctgaaaatta ttccaagatt cttctgtaaa atctccttta
     8641 gcagttgatt ttactcggtt gtataaagct tgtaattcag ctttattcac agtaggaact
     8701 ggttttttct ttaatccttt gtaagcagaa tctaatttat ttttggcact atccacttgc
     8761 ttttgagtcg cttttgagtc atttaacact ttcttagcat tgtttaaagc ggtttggaat
     8821 gcgttccagg tattgtcggt atagtcacct tttttcgttc ccttaacgct cgtataaaga
     8881 gcttctagag ccgatttatt cactttgggt ttagaggtgt cagcgccata ccaaccgata
     8941 ccttcgtatt tccagccata gtctttggtt aaccattgta cttctttcca acttgcagtg
     9001 tagtgatgtg agccagattt tacaccagga aggaaaaggc ggtagatcgg tttattattt
     9061 ttttcggcag aacgccaggc aggcccttca taagtccagc cgtaattttt agtcaaccat
     9121 ttcacttcat cccaactagc tgtgtaatgg tgatcacgaa gacctttatt gtacaaacga
     9181 tagacaagat ggccaccgct agtcggtgct tcccaggcgg ggccttcata atttccccat
     9241 tttatcttta ctagatggtc acgttctttt ttgctagagg tgtaaaaatg ttcattatta
     9301 tttggattgt aaaggcgata catgacgtta gttgaagcag cgtcaacaga tccagcaaga
     9361 ctaagtgagg cagcaaatgt tccaatagct aaaacagttt ttggattaaa taatttcatt
     9421 ttttcaactc ctaaatttaa ttaaaaatgt ttacatgatt attatatatc ggctaaccga
     9481 tatagtcaat aaaaaataag aaaagcctta tataattttg gcttttctta ttttatttgt
     9541 tagttgaagt attaatatca acgatttctt cagccaattt atttaatttc atagcattta
     9601 tgaatctcat gttgtctata ctagagcgtt tgttggcaag gtcacttact gtagttaagg
     9661 gtacgccaga ttctatggat atagaacgaa ctgatttacc actatcaaac agcaaccatt
     9721 cgattttctt tttatcagcg tacatttcaa tgctccttct tgttttttct ttattatagc
     9781 tcttttttgt aatgaatcac aatatcaaga gaaaaatttt ctatagaaag gtggattttt
     9841 atgagtattc gttctccgtg tatccaaatg aggtttgttg ctactttcgt tatttgatat
     9901 actaaaaata aacaacgaaa gaaggaataa aatgaagaaa aagcaactat ggtttgtctc
     9961 tatgtttgca atatttttta ttattttaag tggatgttca agtaataaga aatcggaagc
    10021 taaagaagat aatagtttat tgcaaccagg aactgtttgg aaagaagaag taggtggtct
    10081 tattcataat ttaaaaatta tagatgatac tacctgggaa tattctgaaa gtgtatggca
    10141 tccagatcct gtacaaataa cagttaagag gcaaaaagac tatgaaggtt tagaaagata
    10201 caaaatagta gattctgcag gcattaggga atttataaac aaacctgatt cattatttat
    10261 tgttgttccc tatgagaagg atggcgtaaa aaaaataatt tttcttggat ctagcaagga
    10321 tgagaagcga acgaaagaga aattaattca tgatggatca gagttaaata aatacaaatt
    10381 acaaaagact agtgagtgat tgctagtctt tttatttatc ctgaaggagg aatcacagtg
    10441 gcaaaagtta aaacaccatt cgaaaaagcc gcgtctaaaa tcttagaacg ccgtggtgtt
    10501 tctgaaatac agtggaaaaa agaagttctt gaaaaagcac aatttgacct attacgagga
    10561 aaagataagg acttagaaga atatatctat caacgtgaac gtgaaaaact tgtattcgat
    10621 gaaattatta aacattaaaa ggagcgaata atcttatgtt tgaatcaatt acaatgaatt
    10681 ttttaactca accgaaagta gtagctgcag ttgatatttt tagagcatta caaactgccg
    10741 caactagttt gaaaaataat ctgatgattt ttgctgctgt tgcggcagtg gcttccattg
    10801 ctcttggagg tattttcttc ttctttgggc gaagaggagc tgaaaacggg aagacacacg
    10861 taggttatgt attgttgggg gtaggagtta ttttagctgc cgcttctatt gtagtttcaa
    10921 tgttctcaat gtttggacaa accccacaag gactataaaa aaaacaagta ggaggagtaa
    10981 aaagtacgtg gctacttttt actccttttt ttatgaaagg agaatattca tgttttttac
    11041 gatttcagga aatgcaaaat tggataaaga gttaataaga aaagcaacga agaaagtggg
    11101 aaagcgtact tcctttttac cgaaaacaat taaatattct gtcattgaaa cggaagaaca
    11161 gctacatgtg ttaggacaag tgttagaaca agagaatcaa tcttctgtta aattgactct
    11221 cacgttacat gataaagaag gaaaaataga aaaggctggc acagaatacg tttttggccc
    11281 actggattat caaatttcag aaggttttta tcctacattg atagatttaa tccgccttga
    11341 tgtgtttaaa gatacggatg aaactatgag tgtggaagag tcttatcgaa acgaaaaact
    11401 tttacaagtt ttagaagaag agttagagag cgaccaaaga aaagtgaatc aagtgagtga
    11461 agtcgaagta aatgaagaag taccatggga acgaccaatc tttccaaaga aggagtcaaa
    11521 agaaaaagag acagtctatg ttgacgaaca agctgatatt ccgactcaga aatcgatatt
    11581 tgaggaagag aacgatgtga tgggtcaatt ggaggtagat ccctttgaag aaacaatgga
    11641 ggatgaagag acggacgaag tagaagttga tccagttgaa gctaatgaag agtcaacatg
    11701 gactgaaggg gattttcagg aatatattga accacttatc agtgaagaag tagaaaaaaa
    11761 cgaaacagag atacaaaagg caactgtatt tgattatatt ttggatcaat atccgccaga
    11821 gcatgaatgg atgaaagaaa aaaacaaatt atttttaaat gagctttatt cgaaatatca
    11881 attaccccaa acctatcaag catttttaga gagtaaagaa gaattaatct cacagggtaa
    11941 aggcacactt ttagaacagt tagagtctgt ccaatcaaca aattggcatg agcttgcaca
    12001 agaagattta ttagccacct ttgaagaacg tcaacaagaa acagatcaag aaattaatga
    12061 gtatgcgacg gaacagtcgt ctaattggga gaaagtaaaa gaggaactaa ataaagaaga
    12121 acagcgagtg attgaagaag aagtacaaaa agtcaaagag cggtacgaaa agaaacgcga
    12181 caatgcttat tccttgtgtg tagaaaagat caacaatttt actaagacga atcaagaagc
    12241 gttatcgaaa gaaaaagaaa acttgttaaa agaacgtatt aaagaacgaa aagaagagtt
    12301 ctaccgcggt ttacgtacag ataagctaaa gattagcaaa gaattaaatg aacaactgaa
    12361 taagctatat caaacaacga ctgaagttct tatggaaaaa cacgaacaga tccagcaaga
    12421 attacaagta caaattccaa aatggaaaaa agagtatgaa attcaacaac aagaattgaa
    12481 agaacaagaa gaaaaacaac ggcaaaaaga aaaagagcgt gaacaattag aaataagaaa
    12541 acgggaacag gatcttgcgg aacaaaaaat ggcgatagaa aaacaacggt tagagcaaga
    12601 aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa caaatggata cattgcgtca
    12661 agcacagttg aattatctag cacaacctca accagtagct gctgttcaaa cgccagtgaa
    12721 caaccaagca aatttaactc cttcacccca gcgttccttt aacggatgga tggtgggatg
    12781 tattgcaagt ttaagcttgc ttctaggtgg aggaacagta tttgctttta atcatatcaa
    12841 tcaatcttca caggctgatt ctgtagccac aacggcgtca atcaaggaag aagcccagga
    12901 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    12961 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
    13021 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    13081 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
    13141 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
    13201 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
    13261 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
    13321 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
    13381 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
    13441 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
    13501 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    13561 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    13621 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    13681 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    13741 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    13801 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    13861 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    13921 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    13981 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    14041 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    14101 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    14161 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    14221 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    14281 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    14341 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    14401 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    14461 ggctataaag gatttagtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    14521 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    14581 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    14641 ccagaattag aaaaagaagc cgttctaaca ttagatgagg aaaaaagtaa cttattagta
    14701 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    14761 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    14821 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    14881 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    14941 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    15001 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    15061 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    15121 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    15181 gatcgtatct taactcctga aagaatttct acttttttag aaggagaaac gattgtactc
    15241 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    15301 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    15361 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    15421 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    15481 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag
    15541 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    15601 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    15661 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    15721 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    15781 aagtcttaca agattatggg aattatctgc agtatcaaga tgtatttagt tggatttgga
    15841 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    15901 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    15961 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    16021 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    16081 ttatggccat gatcttgatt tttggtggta gttctttaat cgatgaaggg ttaaatatca
    16141 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggatcacctg
    16201 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac gttactagca actgacccga
    16261 ataaagtaga atccctacca acagataaaa gaaatgcttt aacagcgaaa aattttaaaa
    16321 cagcgaatat caatgggatt attactcctg aacaagctaa aggtatggca ggtaatgata
    16381 aaagtgggtt atcagatata caaaaggatc ggttaaaggc attacaatac cgattggaac
    16441 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    16501 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatttta gctggtgaat
    16561 cttctttggc agtagcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    16621 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggtcaac
    16681 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    16741 tagaattacg aatctatacc ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    16801 agatcaatgg attcttgtat gtcgttgctt tgattgtact aaccattgcg ctattcaaag
    16861 gctctcaatc agttacgaaa attttcggcg ttgatacaag cctgaaaaac ggcagtaatt
    16921 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    16981 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    17041 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    17101 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    17161 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    17221 aagatcttac agaaaaagcc aaagacacct tagaagataa attagacggc acgagagcta
    17281 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    17341 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtc aatgcagcag
    17401 tgaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    17461 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    17521 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    17581 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    17641 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    17701 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa
    17761 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    17821 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    17881 atagtcgttt gaatcaattc aaggataatg atcaaagttt tgttcatcaa tcacctgcag
    17941 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    18001 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    18061 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    18121 ttactgcacc aacaaaaata aatgtgacaa gagaaacaaa aaacaggaac aatgcattga
    18181 aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag
    18241 gccttttttc tatgtatgaa aaggagaaat catttatgga taaatatagt aacatgaaaa
    18301 accctccaag atttagtgga gaatggaagt ttttcgcttt tttcttttta gatctattaa
    18361 cgcttggtgg ctgctttttt tgggcgaaaa tggtcaataa tcttgtccac ttaccactct
    18421 tttttgcact cttgaattat ggcttatgga ttgcgatagc gttagtgatt gtttggcgac
    18481 cctataataa cccaggtcaa cgacaaattt ttgcgattat cgagtctatt ttttttgcgg
    18541 acaagaacca ttatcatgcg ttagatccta accgaaatta tcatggtacg tataaggagg
    18601 atgaataaat gcgagtcaaa ggacaaaatt cagttgaaag gaaatccatt ttgcagtttg
    18661 gaaaaccaga agagcgctct atcttggact tatttccata taaatcctgt acagaagagg
    18721 gagtcttaat tactagagaa gatcattttc aacgttatta tcgtgtagtt tctacagacg
    18781 tggaaggatt aaatgaacaa gagaaactag aacgaatgaa tcagttgacc accgtcatgc
    18841 gtacgtatgt gccaacaatt aagttagtta gtttgaccac agagacggat ctaacggaac
    18901 aaatcgtgca aaagcggcaa ttattagata aaaatcgtat ggaacagtcc ttaggaagaa
    18961 atctacaacg attaaaaaaa tatgaaaaga aattagtcga agaaatccag gaattgaaac
    19021 gagcagaaca agaacgtcct gacttaagct tctttttcgt cattgaagga aagacaatca
    19081 aggatttaca aaccagaaac cgtcagttaa tgcgatctag tggcatttta gggttaaaac
    19141 cactctacaa aaaagaattg atcaagattc tgtatcgaat gaacaatatg aatgatgagt
    19201 aggaatggag aagagagaat gaaatatccg ttgatatggt tagaaaaaga tgttccgaag
    19261 gaagagacca ctagaaaatt tcctcacact ttaagcctgg gtaagttggg aaaagggcaa
    19321 tcttttaacc aagtaaagaa tcaaacaaga agaggtgact gtacatgtta gaagcaatta
    19381 aaacagcttt ttcaccacaa aatgatcaaa aagtacttga tccattaaag caactaggaa
    19441 atcgggaaat agaaattgta aaatccaaag ggtatgattt agatttttta aaacaagtga
    19501 tgccacaagg tgggttaaat tttgatgaag aatatgtgac gtatggtaat ggttattcac
    19561 gatgtgtgac agtttatcac tacacaaaaa atcctacgct tttttggttg gccaatctca
    19621 tgaacaatcc tcatacagtg gccacactcg atgttagcac agatgaaaag caaaaagtga
    19681 ttgatcaaat caatcgtgcc ttagatgaat taaatgatcg atcagataat gaacgaaaag
    19741 cgactgaccg aaataaaagc gcggaagaat acctggaact agcggaatat gctagaaaat
    19801 tgacttctaa tggggaaatt tctaagcaag tcaaagtgag agtacatgtt tacggaaaca
    19861 cgattcaaga agtagatgaa cgagcgcagg aactgatcga aaatctcaaa gggatggatt
    19921 atcgtgcggt gacttatctg cataaaacca aacgagattg gcaatcgatg tttaccatgt
    19981 tcgatgaaca agctgaatgg ttcggaaata tcaaagggca atcactacca tcgttaaaca
    20041 ttggcggagg tttcccgttc caccaccaaa cgctaaaaga tccaggaggg acacaatatg
    20101 gggaaaccat gacaggcggc ccatttgtct ggaatgcgac gtataaaaat agtgtacggt
    20161 tgtctactaa taccttgctt ctaggaatga tgggagctgg taaatcgacc gttcttaaaa
    20221 tgatcgcgga agcccattta gctgcaggag actttatttg gggctttgaa aagggaaaag
    20281 actttattcc tttcttaaag gaatataacg gtattatggt gcgtttagac ggatctgacg
    20341 gaatgatcaa tcctttagag atatttgcca cacggacgta tgatgaagca tccagtttat
    20401 atgacgatgg atcagtcaaa gatttgaaga tcaatgaagc agcgtcctat caaacacact
    20461 tagacaaagt ggtctatcag gtacagttag tgagtcctca gctaaaagga acgatgaaag
    20521 cagaatttaa gacctactta aatcgttttt atgaagagta tggaacagta ccacgcggct
    20581 ttacttcctc aaattcgcgt tcaaatactg atacgcaagt cacagggaaa gatccagaag
    20641 cttatccgac gttcaaagaa tttttagact ttttaggaca actggaatta ccaggtgctt
    20701 cccaagagaa aaagaatcga aaagaagaaa tggaatccat cgtagaatcc ttatgtgaga
    20761 cgtatggcat gatttttgat ggtcatagta cgattcgaca ccttgaccaa caacagttgg
    20821 tcttttttga cattgactct attacaggct tagatcgagg ggtacaacaa tgtcaaatgt
    20881 acatggctat gacgttgatt tggaatcagg cattgattca aggaagaaga caacgacgtt
    20941 taattaaaga aaaacaaatg gaagaacaag acttcaaacg cttcttggcg atgattgatg
    21001 agtgccataa tggtgcgacc tgttctgcag tgaaaagctg tgggttgcaa gtagtctaaa
    21061 aactattgta ataactctta taacgagaag tcaaatgagt gggtaacgcc acaaagggct
    21121 tccactgata ctccgacatg caacaagtag tttatgcaga ccaacgttgt gtgaagctcg
    21181 gtgaagtcgg ctgaaggata ccgtaaggta aaagaaatag tgaataccac gaaagttctc
    21241 tagaggtaaa gggtgtatcc gataagtcgg gtgtccaaaa aaacttaata tggtgagtat
    21301 gtacaaacgt actgacgaac tagcgaatgt actgtccgaa aggcatagct cctagaaatg
    21361 ggagagacag tgagcaaaaa atcactcatt gtgctaactt gtggttgagt aaaagtcgtt
    21421 gttatgaaag accatgctgc gttacaggcg taggagaagt tagggggcaa aagctagcat
    21481 ctaagttaag gcatgtggat agatataagg gaacagggga agccatcaac atggagagtg
    21541 catactcgat gaagtggtag taagaaatat gtgaatgaca aataacttac tttagtgatg
    21601 gtgaaagtag agtcacgact gatgaaggtt ctgtaatgga actggaggaa caggctcgat
    21661 tcgtctgtaa aagtcatgaa acccgttcaa ccatgggatt ctcgtatgac taaaaaggtc
    21721 acgagccaat tggagcgtga taaaccatga caaaagaaac aagtccaaaa gataggaagt
    21781 tgcggcatgc agaatattat ggcatgacgg aaatcttcga taagatatac tccaaaagtt
    21841 tgaagggaga taatttcaag aatctaatgc agattatcat ttctgataac aacattgtcc
    21901 tagcatatcg taatatcaag agaaatcacg gtagtgtgac aagaggagaa gatggagtaa
    21961 cgattaaaga tattgaaaat ctcaaccgta atgaattcgt agaaattgtt aggaagcgat
    22021 tcgcacatta taaccctcgg aaagtaaggc gtgtagaaat tcctaaatat aatggtaaat
    22081 taagaccgtt gggtattcca tcaatatggg atagaatcgc gcagcaatgc atcttacaag
    22141 tattagaacc aatatgtgaa gcaaaattca ataatcacag ctatggtttc cgcccaaata
    22201 gatcagcaga acacgcaata gcagattgta tggcccgaat gaatcaaagt cacatggaat
    22261 atgtggtaga tgtagatatc aaaggtttct tcgacgaagt caatcatact aaactcatga
    22321 gacaaatctg gtctatgggt atacgagaca aacagcttct cttaatcatt aggaaaatgt
    22381 taagagcacc cgtagtactt ccaaatggga agttgatata cccaacaaaa ggaacaccac
    22441 aaggcggaat acttagccca ttacttgcga acatcaatct caacgaattt gactggtgga
    22501 tagcaaatca gtgggaggaa aaagatgcca aagagctaaa acatcatttt aggaaaaatg
    22561 gagcttggga taaaggtaat atctatcaca agctcagaaa gagtacaact ctaaaagaaa
    22621 tatacctagt gagatacgca gatgatttta aaattttctg tagaaattat aatgaagcga
    22681 aaaaagtttt ctatgcaact aaaatgtggt tacaggagcg tctaaagtta ccaatatctg
    22741 aagaaaagtc aaaaatcaca aatctttcaa aagaagaaag tgaattttta ggctttacga
    22801 tgaaattagt gaccaaaggt aacaaaaagg ttgttaatac gcatataggt acaaaagcac
    22861 aaagcatgat taaagtgaaa ctaaaaaaac aaatcaaacg aattcaaaaa agtcctaata
    22921 gcaaagatac ctttatctat attggagtct ataacagtat ggttattggg attcataatt
    22981 acttcaaaat tgcgactcat tgtagtgtag caatgcgaaa aattgcaaga gaactcagac
    23041 caagttttga aatacgacta cgagaacaag gaatctcaaa agaaggacaa tacggaaagt
    23101 acgaaggata caagccttac ctacggtcag gacaaatacg atatataaga gggtttccaa
    23161 tattaccagt agggtatatt caacacagac atccgatgtg taaaaaaaga aatattaata
    23221 aatacaccga agaaggtagg gaagccattc ataagaagca acaaaccgta gaagaatgga
    23281 agatatcgtg gttgtgtagc aatccagtgc ttagtaagag agcaacgatt gaatttgcgg
    23341 acaatcgaat ttcaaaattc atcgctcaaa agggaagatg tgcagtaaca ggtgaggagc
    23401 ttatcttatc tgaaatgcat tgtcatcata ttatccctta tcatgaaagc aaaagtgata
    23461 gctatgaaaa cctagtgatt gttactgagg aggttcatcg ggtaatacac gctacacaaa
    23521 gtgaaacgat tgaggaactt ctaaaatatc taaaattaaa tccaaaacaa aaagaaaaac
    23581 tcaatgagtt gagattaaaa gttggtaacg aagaaatatc ttaataaata tttgtaggac
    23641 gggaaaacag aatgtctttt atagacgagg gaacgccgta tgcggtgaaa gtcgcacgta
    23701 cggtgtggac tgggggaaaa gacggagata atatcaaact cttacctatc agtatcgatt
    23761 aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga aatgcgtaaa
    23821 gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc agaaaatatg
    23881 tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac gaagatcttt
    23941 atgcgtatgg atgaatctat tttacgtcat atagaaggat tagtgggtaa atcaatgacg
    24001 aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat caattttggc
    24061 tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa attgtttgat
    24121 ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa gcagtatgtt aagcgtcgtg
    24181 caaagcggcg cttatttttg tggttgtttg gtacttctgc aggtttgatt actattttga
    24241 tcactgtgtt cgtgacccta tttctaatac ttgcggcggg ctcgattgat aattcggatt
    24301 cagattcaag cagtggtggc gaagctttta caggagaata ttcggaaggc ttaccgattt
    24361 ataaagaaat caaaggaaga ggcccttttt ctgatgagat tgcgcaatat gccgtaggtg
    24421 cagcagtgaa atataaatta ttaccaagtg tgatcctttc gcaatacggt tacgaatcag
    24481 cgtttggtac atcggcgtcc gcaagaaatg atctcaacta ttttgggatt acttggtttg
    24541 atggttgtct gtttcctaaa ggaactgcac gaggaattgg tggcattgaa ggtggctggt
    24601 acatgaagtt tcccaactca aaagctgcat tttcctatta cggctttatg gtagccacac
    24661 aatccaattt taatgcttgt gtaggaaata aaagccctgg cgcaagctta ttgatcttag
    24721 gacgaggcgg ttatgccgct gcaggaatta cggaagatag tccgtattat acaggttgta
    24781 tgtcaattat cacttctaat aaattaaccg aatatgatga attcgccatc aaacattggg
    24841 gtgaaggtgg caataacaac ggcacgatta ctggtgaatg gacgaatcca ttccctggca
    24901 gcagtttaga taaaagttcc ttcagcggtg gtcaattatt tggaacgaat ccaggcggtg
    24961 aattccgtcc gaacggcttc catgatggat tagattttgg ttcagttgat catccaggaa
    25021 gtgagattca tgcggttcat ggcggaaaag tcgtgtacgt tggcaatcct ggtatttcag
    25081 gtttgggcgc gtgtgtgatc gtgattaatt acgatggctt gaatatggtg tatcaagaat
    25141 ttgcaaatag tacaggaaat tctcgagtga aagtaggcga tcaagtgaaa gttggtcaag
    25201 taattgggat acgagataca gcacacttgc atttaggatt tactcgaatg gattggcgtc
    25261 aagcacaagg acatgcattc attgatgatg gtacatggat cgatccgtta ccatttttga
    25321 atagcagtaa aaaatagatc aggagaaaaa atatggaaaa taaaacgtta aaaatcgtct
    25381 taacagctgc aattagcgtt agtttaatcg gcaatattgc gttgttttgg cagctgaaag
    25441 aggaacaagc agcaaatacc tataaaattg aagaagtggc taaaaacgcg tctgagaagc
    25501 tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa gttaaatcaa accatcaatg
    25561 agaaaaacca agaaatcgat cagctgaagc aacaagatgg cacaaaagca aaagaggaag
    25621 taactgaagc acaaagaaag acggcagaag agttcggtca attggccatt gataaatcat
    25681 taagcagaaa tgaattaacg gaaaaactaa aagatgtcgc aacccaagaa gtgatcgaca
    25741 aattaagtcc tacagacgat gatcaccaac atgatgatta cggaagttac tcctttacat
    25801 taggtgacgt gcaaacttac gctcaaacaa gcagtgtaaa agaggaacaa aattttgtcg
    25861 tatttgttga ttatacaatc ggcaatccgc aattcaaaga tgtcaaacca caaaaaataa
    25921 aaggtggatt aactgtcact gaaaaacaag tagatggtca atggaaagtc atagacttta
    25981 gttatttcac tcgttaggag gaagaaaatg agtaagaaaa aaggagaaag acaacgtttc
    26041 aaaaaacgaa aaagttatct tagatggctg ggaatcttta tgattctcgg ctttttgatt
    26101 cttgctagtc cgatttatac gaatcagaag gtagatatac cagatgaggt aagcacgaac
    26161 gcctttttaa acacgaaaga gctgacaatg gtttcaaaag aaaaaggagc gaaccagcaa
    26221 ttagtttttg aatttgcgat cgatgatgaa gatcaaagct tgttaaaaga actggctaat
    26281 cttaattatc gcgtagaagt aaagacagct aaaggggatt atcaagcaat acgagccaaa
    26341 gtgattaagg tgtctgatga ttattttgca gtgaagctaa cgcatgtacc agaagaatat
    26401 atcgccatgc gtttaaccat tattcctgaa aaaattgatc caaaagtgga tatgcaagaa
    26461 ccgcaagatc tgatttttta tattcacgaa gataaggtca aagatcacgt gaaagacgaa
    26521 gattatgaac aacatgcgat caattacaaa gtgaaaggat acaagaaaga acaaaaagca
    26581 gcacaaaaac aaattgaatc cttccaagca accattgatt tgaatgaaga attagtgaag
    26641 aaactaaaag atcaattgcc ttatcaagtc gcagaagatc aggaaaatac tgaaaataag
    26701 attaatagtt accagcaaga aattgaaaca tcgaaaacgc aaatgaaaga tcaagaagag
    26761 agcattcaaa aacttcaaga aaaaattgat cgtatgacaa aagaaaatat ttagaagaat
    26821 aggtggagta aaagaaatga agcgagcgaa cttggtcggc aaagatactt tcgctgagca
    26881 gaaagtattg attaaagcct atctcaaagt tgtaaaagct aaagaaatat gtcaagaagt
    26941 gaattatcta acacaacaat atctgagttg aagaatggta gaaagagttt agatacctta
    27001 aaattcagat catttgaagc tctatatata gctgctaaaa aatattttac agctgataat
    27061 aaataaaaaa ttaaggtggg ttctatgtga aaaaaacgta tcaaaagttt ttatggatta
    27121 gctatatcgt cctatttatc gttgggtttc tcgtcagtgt gtccgtgatc gtcggaacgc
    27181 tttttctttt gttctctgac gaagatctgg tacttaaggg gttaatgagt acgttatcgt
    27241 gtgtcatgtt actttattta tgtatacaga tcaacgaaat tatgccccct cattggcaag
    27301 aaaagaagaa agaatttaag gaagaaatcg aaataaaatc caatgactgg attgaataca
    27361 catggcttcc tctgaaaaga aatagcaaac gaaaggaatt ttgggcattg caattatggc
    27421 caataggaaa actttttatt tatatggctt cacttatggt tactttaatc ggttatttgg
    27481 tggtggtcta tggggtggcc tattacttgt taagtgagcc aaaacagatc gcggcgtcaa
    27541 atattttcac ggcagtaatg acattaatgt gtatcatgtg cttttctatt tttagttact
    27601 tactcgtcca atttatcagg caaaatttaa caatgagtaa acttttcaaa agtttattgg
    27661 acttacttat cttaggcagt atgttaacga ctctaacaga tcggttcgaa caatctgaaa
    27721 aaacggtaga ggggataaag aacttcttaa cgacgattga tcaatttata aatggatata
    27781 tgtatttgtt tgaagtaggc tttattctca ctttgtttgg ttgtgtattt ataaaactca
    27841 ttaaaagatc aatcgaaaaa gaaatggtta ttgatctaat tccgacgctg agcggctatg
    27901 agtattacgt tgattttgga aagtatgagg agttgatcca tacggatatc gttcacacga
    27961 gtatccatga agtaaccgtt aagggcatca gatctacttt aaaagatgga caggagacga
    28021 aaacaatcga ccgaagaaaa gaggtctaca atttttataa agttgaagca agtctacgat
    28081 atggcattgg gaaattgagg ttagatggga gtaatattca acaaaacaaa ccatatgttt
    28141 taggaaaagt ggtgatttag aacttcctat gaaagatgaa taaataagga gaatcttttg
    28201 aaaatgattc ttctttacag aattgtgctt atagttggca cggagttaaa aatcttaaag
    28261 aaggagcagt tattcaaagt gaaagaacat cataatcagt tactatgtat taaaacgggt
    28321 acgatgaaca ttcaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg
    28381 tcgattcgag agcaacataa gcaagaaaaa ttaatggcta cttatagcca attgattgat
    28441 gagtgggaac aattcaagga atctaaaaca taaaattcca tttaaacacc ttatttttta
    28501 aagagcataa ggaaacgtga ccccactttt aaaaaaagtg gttggatagg ctgtgcctat
    28561 cacgtttctg aaaaaaataa atgaccccaa aataccaata tataggatag tacaaggata
    28621 gcaaaggata gtaaaaggat agaggtagga tagcgaaaag gataggatcc ttttcactat
    28681 cctatattat attggaggat atttatgaat gttaaaatta gagatttaga tccaaaattc
    28741 atttctgaaa ttgatcgaag atgcgttgaa ctaagtaatc gtacaggaaa taaatggagt
    28801 cgtaatgatt atttaaagct actcgttgaa aatgattttg atcgtccttt gatggaatac
    28861 aaacaagaaa agtttgacca gttattagaa aagttttcag aaattcaaag ttataacaca
    28921 aagatattag aagaatatgt tagccaaaat aatcgtatta tagaaatctt gattgagcaa
    28981 aatagaggaa gtgaactttg atatggcaaa ggttatagac aaacacattt actttcacca
    29041 aacacatatc gatgtattgg atgaaactat ttctactcat gcagaggtta aaaactatag
    29101 tgaagctgtt cgctttttat gtatgaacta tgatccagaa gtggagaaaa aagtaaattc
    29161 caatcaagca aagttgaatg gaatgagtaa agaaatttct attttaaccg aaatggtttc
    29221 agaatttgcg gatcttcatt tgaaagatac aggaatatta attggtaaaa attcaactgt
    29281 ttatcaagaa gcaaaagaac gagtggaagg aaaaattaaa tccaatcaaa caaaaaaata
    29341 ttcccctcag ttgaagaaac ctgtgaatga atctgttcat gccagagtag atccatttga
    29401 tccaacgaag attctaaaaa gataaaaatg aaggaggata ataatgactc aatcgccttc
    29461 agttattctc acttgtcaat ttactttgcc gaataaaaag tcattttcta cttatattga
    29521 ctatattaca agggaacagg ctcttgaaga aaaggaacga cgtacgcccg aagaagaaca
    29581 ggaacttcaa cgaatcaaga atgctttaga ggagttttat attcctgaag gagaaacttt
    29641 ttcggagagt aagacgggta aagaattcag tgaaacgtcg aatgaagctc aatcgttgat
    29701 ggaacatgga atcaattttg acaagttaga ggaacaagat tttaccaatt atctttctta
    29761 tatgacacgt cactatgcgt tagaacaaaa gaaagaactg acgcaaagtg aacaaaaaga
    29821 ataccaacgc ttgaaccaag caatgaaaaa atatcaaaca gaaccccata aaaaagcttc
    29881 aggaaatctt cctggagttt tttctatagc ttcagacgaa gtgaaaggaa gtgatctgaa
    29941 ggagattaag caaatcttcc aaagaggaca acaaaaaggc tcaatcattt atcaagatgt
    30001 tgtttctcat gataattcgt atcttgaaaa aataggattg tacgatccca aaacagatac
    30061 tttagatgaa gaagggttaa aagcagctgg aagaaaaatg atggagacat tattcgagaa
    30121 agaacaattg aatgaaacag gttactggat ggcaacgatt caccgtaaca ctaaacatat
    30181 ccatattcat tttgcagttg ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga
    30241 aaatggtgtg agttatatcg taccgaaagg taagagaaga caagcaacat tagacgcaat
    30301 gaagaccaat tatgtacatg agttagaacg tttttcttat gaaaaggatc gaatgattgg
    30361 tatggagaaa cgaaatttat tgacgcggaa aagtgatttg cgtaacacct taacaaaagt
    30421 ggtaaaagag ccagcgcgtt acgatcgaca cgcgatgaac ttattaaatg aagtatataa
    30481 aaccctacct gacaaacgtt cggaatggaa ttacggggat gaaaaacgaa ccaaactttc
    30541 accatacaca agagagaaac tcgatgagtt aaccaaatat gttttaacaa aagaacctga
    30601 atatcaagaa tatatacaga tttcaaaagc attgaaagaa gaagcaaaaa gtctttatgg
    30661 ggaaagcaag agagaatcaa aggatgcaga gaaaaatgct ctctttgatt taaaaaagcg
    30721 cacaggaaat gccatcctaa cagagctgaa gagacaggat agtcaaataa aaccattaat
    30781 agaaagtgtt gatcagatgc aatctattta caatgaacga tcctttcgaa cgcttaaaca
    30841 attgccaacg aatgaataca aatatagtta cgaagaattc atgaagcgca ggaaacaatt
    30901 ccaacaaaga gttgtcacgc agcggagttt gcgtcaatta aatcgaacga ttaatgccca
    30961 tgaagaaaaa taccacgcaa gtaaagcata cgaagagcaa caacgaagaa ttcaaatgga
    31021 acaagaaaga cagtaaagac agtaggagga atagcaatga aacaatattt agatcaatgg
    31081 aaagttatag aaggatcatt aagagaagaa cgaatcgaac aacttcctga ttgtttagaa
    31141 aaagagcatt tgttccagat aagagaaatg ttgagaaacg aacagtttga tccaaatcaa
    31201 tttttagtcg ttgagtaccc agctactggt gtgtattgtt gtaatcatgt gaatggtgaa
    31261 aaatatttta tcattcaaga atatgagggc aagttagcac cttattatac tacttgggaa
    31321 atgaatgaag agggaatcaa taactttcca tgtaaaagca
//
LOCUS       DACCQI010000016        31308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCQI010000016 DACCQI010000000
VERSION     DACCQI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.016"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IVO76_000592"
     CDS             complement(45..392)
                     /gene="rbfA"
                     /locus_tag="IVO76_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7428579.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(419..2725)
                     /gene="infB"
                     /locus_tag="IVO76_000593"
     CDS             complement(419..2725)
                     /gene="infB"
                     /locus_tag="IVO76_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7428580.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2738..3049)
                     /locus_tag="IVO76_000594"
     CDS             complement(2738..3049)
                     /locus_tag="IVO76_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7428581.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3046..3339)
                     /locus_tag="IVO76_000595"
     CDS             complement(3046..3339)
                     /locus_tag="IVO76_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7428582.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IVO76_000596"
     CDS             complement(3361..4536)
                     /gene="nusA"
                     /locus_tag="IVO76_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7428583.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IVO76_000597"
     CDS             complement(4560..5033)
                     /gene="rimP"
                     /locus_tag="IVO76_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7428584.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5177..9529)
                     /locus_tag="IVO76_000598"
     CDS             complement(5177..9529)
                     /locus_tag="IVO76_000598"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7428585.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9741..11450)
                     /locus_tag="IVO76_000599"
     CDS             complement(9741..11450)
                     /locus_tag="IVO76_000599"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7428586.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IVO76_000600"
     CDS             complement(11518..12786)
                     /gene="rseP"
                     /locus_tag="IVO76_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7428587.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12947..13747)
                     /locus_tag="IVO76_000601"
     CDS             complement(12947..13747)
                     /locus_tag="IVO76_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7428588.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13744..14556)
                     /locus_tag="IVO76_000602"
     CDS             complement(13744..14556)
                     /locus_tag="IVO76_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7428589.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IVO76_000603"
     CDS             complement(14751..15308)
                     /gene="frr"
                     /locus_tag="IVO76_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7428590.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15311..16033)
                     /locus_tag="IVO76_000604"
     CDS             complement(15311..16033)
                     /locus_tag="IVO76_000604"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7428591.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAHMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16169..17050)
                     /locus_tag="IVO76_000605"
     CDS             complement(16169..17050)
                     /locus_tag="IVO76_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7428592.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IVO76_000606"
     CDS             complement(17149..17931)
                     /gene="rpsB"
                     /locus_tag="IVO76_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7428593.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18290..18769
                     /locus_tag="IVO76_000607"
     CDS             18290..18769
                     /locus_tag="IVO76_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7428594.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18986..20077
                     /gene="phnW"
                     /locus_tag="IVO76_000608"
     CDS             18986..20077
                     /gene="phnW"
                     /locus_tag="IVO76_000608"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7428595.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20070..20855
                     /locus_tag="IVO76_000609"
     CDS             20070..20855
                     /locus_tag="IVO76_000609"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7428596.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(21201..22892)
                     /locus_tag="IVO76_000610"
     CDS             complement(21201..22892)
                     /locus_tag="IVO76_000610"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7428597.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IVO76_000611"
     CDS             complement(23314..24261)
                     /gene="arcC"
                     /locus_tag="IVO76_000611"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7428598.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IVO76_000612"
     CDS             complement(24376..25395)
                     /gene="argF"
                     /locus_tag="IVO76_000612"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7428599.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IVO76_000613"
     CDS             complement(25486..26715)
                     /gene="arcA"
                     /locus_tag="IVO76_000613"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7428600.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            27176..27877
                     /locus_tag="IVO76_000614"
     CDS             27176..27877
                     /locus_tag="IVO76_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7428601.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(28449..29465)
                     /gene="tsaD"
                     /locus_tag="IVO76_000615"
     CDS             complement(28449..29465)
                     /gene="tsaD"
                     /locus_tag="IVO76_000615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7428602.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(29462..29926)
                     /gene="rimI"
                     /locus_tag="IVO76_000616"
     CDS             complement(29462..29926)
                     /gene="rimI"
                     /locus_tag="IVO76_000616"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7428603.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(29933..30475)
                     /gene="rimI"
                     /locus_tag="IVO76_000617"
     CDS             complement(29933..30475)
                     /gene="rimI"
                     /locus_tag="IVO76_000617"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7428604.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(30459..31283)
                     /gene="tsaB"
                     /locus_tag="IVO76_000618"
     CDS             complement(30459..31283)
                     /gene="tsaB"
                     /locus_tag="IVO76_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7428605.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
BASE COUNT         8491 a         6716 c         5523 g        10578 t
ORIGIN      
        1 aatcccgcaa cccctgattt cacaggattt gcgggatttt ttgattagtc tttgttcaag
       61 tcgcgaagca attcatcgat atggttccca tagacaactg attcatcaag ttcaaagaac
      121 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
      181 gctttattca atccatcttg tgcttttttc ttatctgatg ctaagtcaga taaaatgctg
      241 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
      301 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
      361 tgaccgactc ggcgatcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
      421 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
      481 tgaatttttc aatcatcgca ccacattcat aacccatttt gacttctttc acgtcatctt
      541 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
      601 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
      661 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
      721 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
      781 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
      841 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
      901 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
      961 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
     1021 cttctttcaa ttcgccttct ttcaggcttt caaataagtt gtccaaagtc acacggcttg
     1081 tggcagcacg gttttccaat aatgcacgtt ttgcgcgttc ttcacccgct gcacgtgctg
     1141 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
     1201 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
     1261 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaacg tgaagtgttc
     1321 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
     1381 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
     1441 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaaa
     1501 tcgtttctcc accccatgct tcaggaatta attcatactc acttaattct tgcataacat
     1561 gctgaggatt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
     1621 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
     1681 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
     1741 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
     1801 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgcaatgtat
     1861 ccaataaggt tgttttcccg tggtctacgt gtcccataat cgttactact ggcggacgtg
     1921 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
     1981 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
     2041 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
     2101 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
     2161 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
     2221 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
     2281 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
     2341 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
     2401 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
     2461 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
     2521 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
     2581 cttgttgcaa tttcttctca tcactgctag taatcgcacc catatggttt ttcacatcga
     2641 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
     2701 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
     2761 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
     2821 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
     2881 cttttatctt ttttcttgta ttctcacttg catcggttgc cacaaacacc aattttgctt
     2941 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
     3001 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
     3061 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
     3121 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
     3181 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
     3241 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
     3301 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
     3361 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
     3421 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
     3481 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
     3541 agtcaattta gcagctaaac gtgcattttg tccgcgttta ccgattgcta gcgataattg
     3601 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
     3661 gacttgtgca ggattcaatg catttgcgat gaatacagct ggatcttcat tccattcgac
     3721 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
     3781 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
     3841 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
     3901 tacttcttgt tcaaataagc gacgcaatag gtctggatgg ctgcggctaa caaatacttg
     3961 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
     4021 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
     4081 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
     4141 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
     4201 ttgtttagcc gtttgcgcag cgatccgtcc aaaatctttt ggtgttactt caaaacggat
     4261 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
     4321 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
     4381 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
     4441 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
     4501 taaagcgtct aacgcattta acatttcttt actcattttt ttctgattcc tctccgtgat
     4561 taaaattgga tagctaagcg agcttttgca atattttttc ggtcaaaagt caattctttt
     4621 tctcgtgttt ttatgcgtat tttcaatgta agctgttcag catcaaatga ttgaagaaat
     4681 ccttcatatt gtttttcgcc atctactggt tggtataagg agacatggac ataatcacca
     4741 agtgcctttt catagtcgct ttcttttttc aatggccgct ctgctcccgg ggaggatacc
     4801 tccaggaaat aagcttgagg aatcggatct ggatctgctg catcaagttt ttcactcagt
     4861 ttctcactga caaaggcgca ttcttcgata tcgataccgc cttctttgtc aataaatact
     4921 cgtaagaacc agctttttcc ttcttttaca aattcgactt cgacaagttc aaaattttgt
     4981 tcatctaaaa tcggtgtcac catttctgtg actgtttcaa cgacgctact caaaagtttc
     5041 gcctccttaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
     5101 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
     5161 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
     5221 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
     5281 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgtttcg
     5341 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
     5401 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
     5461 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
     5521 ctaatccctt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
     5581 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
     5641 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
     5701 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
     5761 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
     5821 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
     5881 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
     5941 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
     6001 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctaa catccctcga acaaaacgag
     6061 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
     6121 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
     6181 gtgggtcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
     6241 gaatatctaa tttcaataca ttatcgtgaa tcgagtggaa atcaaaatga gtcgttttcc
     6301 attctgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
     6361 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
     6421 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
     6481 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
     6541 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
     6601 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
     6661 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
     6721 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
     6781 gacaataata atgaggggct aatggattaa cttcagtgat tcctgtcatc gtcgcaacaa
     6841 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
     6901 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
     6961 ctttttctaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
     7021 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
     7081 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
     7141 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
     7201 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
     7261 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
     7321 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
     7381 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
     7441 taacttcaat ataatcatag aattttgctc ggtttcttgc ctcttctacc cctttttgca
     7501 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
     7561 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
     7621 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
     7681 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
     7741 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
     7801 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctaacacca aactttttag
     7861 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
     7921 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
     7981 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
     8041 acatccgcaa tacttcttct tccgactttg accctcgaac catttcatcc gtgataccag
     8101 tcagatctac cgttgttcgt gacaatggat gtcctggatc gataaattca tcaaagcttt
     8161 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
     8221 cagacaatcc tgtcgtttcc acgtcaaata cgacgtatgt ggcttcactc aatgcttcat
     8281 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
     8341 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
     8401 catggtctgt aatggcgata gctcggtgtc cccattttcc tgcttgtgcc acaagatcag
     8461 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
     8521 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
     8581 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
     8641 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
     8701 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
     8761 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
     8821 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
     8881 tgggtccttc aagagctggc gattgctctt ttctttcctt tttcttttgt tcgtggacaa
     8941 ctagactttc agctgcttgc ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa
     9001 aaagtttcaa tacacgttcc gcttgctgct catccacttc tggttcgata cgaaattttg
     9061 ggaatccgta actgacgaac aactcttcta ctaatggcaa gtattgttgc ttcaaataag
     9121 aaatagctcc tgtactgtcg attggcaaga tgactttacg atcttttatc acaggtacct
     9181 gcgttttcaa tacttgctgt gctagtggtg tatcgcattg ctgattttcc aaagcttggg
     9241 cccagtaatc ttgcaagagc tgttcatcga atgtctggtc atctgctaag atattcagtt
     9301 tgacttgtgc aatatcttta aaagcgatag tcagatgctg agtcaatgac cgatatagca
     9361 taataggcaa aattttggaa aaatgtagcg taaattccca taaacgcgat ttacgatgga
     9421 ccactacact atccatttca ccagcagtaa gcagtggatg atttttttct gcttcctcta
     9481 gttggatttg ttccaacagt ttttcaaata aatctcgttt ttctgacaaa gatatacgcc
     9541 ctttctgatg agaaacaatg caattgatcg catcttatcc tcttcttttt cagtaccttt
     9601 tagtatactc gttttttctg agttttacat taaaaaattg gaactgcgat tcaaatcgca
     9661 gttccaattt taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag ctatttttat
     9721 tttctgtgtt ttaacgctta ttattcgcct tggttcaaga ggattggtaa agtattcgcc
     9781 aattcttctt tacgtacttc aaccatttcg ccagttttct tgattttcac ttctacgatt
     9841 ccatccactg cttttttacc gacagtgatt ctgattggac agccaatcaa atcagaatcc
     9901 gcgaatttca ctcctgcgcg ttcatttctg tcatcaacca gtacttcata gccagctttt
     9961 gtcatcgctt cttctagttc attcgtcagg cttgtttggt attcgtcttt tagattcatt
    10021 tggacgatat gcagatcaaa tggcgcaatt ccttttggcc aattgattcc ttgttcatct
    10081 gaatgttgtt ctacgattgc tgataataaa cgacttacac cgataccata acagcccata
    10141 atcacaaatt tttctcgtcc attttcgtct agtacagttg ctcccataga ttcactgtaa
    10201 cgagtgccga ttttgaatat atgtccgatt tcgattcctc gagtaaaggc aagaacaccg
    10261 ttgttgtctg gtgaaggatc tccttcttgt acaaaacgaa gatcttcata agaaagcaca
    10321 gtgaaatcgc gttttggatt cacattttta tagtggaatc ctgtttcgtt cgctccagta
    10381 atggcattcg ctaaatcttg gacataacgg tctgcatata cttttacgtc atctgaaaca
    10441 ccgactggtc cgacagaacc aaactcagca cctagatatt tttgtgcttc ttcttctgtt
    10501 gcttcttcta aaaagtcagc agataaaaag tttttcagtt ttacgtcatt gacttcatgg
    10561 tctccacgta ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac agacttgatg
    10621 attttttgag gttcaacaga aaagaaatct gctacttcgt caattgattt tacgttaggt
    10681 gtagctactt tctccaattc aagctgtgtt tcatgcgatt tttttgatac atagtagctt
    10741 gtagccatct ccaagtttgc agcataatca ctttcagtcg aataacagat cgtatcctcc
    10801 ccgatttctg agatcgccat gaattccttt gagtctttcc cgcccattgc gccgccgtct
    10861 ccgatgatcg agcgaaattc taagccacaa cgcttgaata cttcggtata ggctttttcg
    10921 taatctttat atgtctcgtc caaactttct tccgttgcat ggaaagaata tgcatctttc
    10981 atgataaact ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc acgatattta
    11041 ggttggattt gataaagatt taaaggtaat ttcttatatg aattgatttc attacgcatc
    11101 aaatcagtaa aagtttcttc gtgagttggt ccaaggatca tttttcgatc attgcggtct
    11161 ttgaaacgat acaagtttgg tccatatgtt tcgtaacgcc cggattcttc ccaaagttct
    11221 gctggcaaga tggcaggcat cagcatttct accgctccga ttttttcaaa ttcttcccgc
    11281 ataatgcgtt tcagtttttc caatacacga tttgctaatg gcagatatga ataaataccc
    11341 gctgatactt ggcggatata tccagctctt agcaagattt gatggcttaa tacttctgca
    11401 tcatttggaa cttcgcgtaa tgttggaatc aacatttttg attgtctcat tctatatact
    11461 cctttatttc ctcaaaatag ttatcgtttg ttattatacc ctatttgtca actttttcta
    11521 gaaaaagaat cgttgaatat cgttccatgt aactagcacc atcaacaaca ttaaaaatcc
    11581 aaagccagct aatgtcaata ttccttcttt ttcttgactt aatggtttcc cgcgaatccc
    11641 ttcaaaaata ttcaagacta atttcccgcc atctaaggca ggaatcggaa gcagattgac
    11701 gattccgaga ttcattgaaa gaagcgccat caaactaata acagttgtta caccttgatt
    11761 tgctgcttct gaagacaatt ggtacatcat gacaggtccg cctaatttat ctaaactgaa
    11821 acctgtaaat agtgatccta aggctttgaa aatttccaat gaaccgctga aagcttgacg
    11881 cgtccctcca atgatcttat ccataaaacc tgtattcatt ggggctttta taccaagttg
    11941 gccaactttt tcgccatttg actctacaga tttcggtgtc acgtcgacag attgcacctg
    12001 accttctcgt tcgatcttaa agtctaatgt tttacctgga tttttcgtaa tgactgttgt
    12061 cagatcattc catgaatgga tttcttttcc gtccacactg actacctcat cattttcttt
    12121 taagcctgct tctgccgcag ctccattagg catgatctct cctacgcgat tagtgttcgt
    12181 cacttgaaca ccaccttgca taaatgccag aatgatgaat aacacgattg ccaaaataaa
    12241 gttattcatg ggtccagcaa agttggtcag catacgctgc cacaatttag ccgattggaa
    12301 ctgtacgtct tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc
    12361 gtgatcaaca gggtaggtta ctacttctgt ttcgtctcca ttcacataac cagtgatagt
    12421 cagctcatct tctaaatcat aacggctcaa ttccatcggg atagcatttg tcaattggat
    12481 tttcttactt aaatttatct tctctacgat gccgtcagaa tttagtaaaa gagaaagagg
    12541 cattcctggt gccatttctg tttcatcatc cccattgcct gccattcgaa cataaccgcc
    12601 aataggtaat aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc cataaatttt
    12661 cggacccata ccgatagcaa attcgcggac gaggattcct gatcgtttcg caaagaagaa
    12721 atgaccaaac tcatgaacaa tcactaatat accaaaaacg atgataaatg tcagaatcgt
    12781 tttcatagct tttttctcct ttacttgttt tttgtgtttt ttataaaaca acgataaaac
    12841 actataattt taccatagta taccaaagac accaatagta tcaatataat gaaaaataaa
    12901 aacctgcgtc ttttatgaaa ttcacaaaga aacgcaggta tacttactaa ataatgccaa
    12961 ataaatgcat gataggaaat acaaacagca aactgtcgaa acggtctaag attccaccat
    13021 ggcctggtaa gatatttccc gagtctttca catcataatg acgtttgatt gcagattcga
    13081 ccaaatcacc aaattgtccg actgctgaaa aaataatcgt catcagaagc atgacaaaca
    13141 taccatgatt gaatagttct tttgctgggt atagcaataa ataaagaaat gcgactacaa
    13201 cagcactacc gataccaccg agcgcacctt caatcgtttt gttgggcgaa atatcaggac
    13261 ttaacttatg ctgaccataa cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa
    13321 caataaatag accaaatatc aaaacagcca agctttctgt tcgtgcattg acaaaattct
    13381 ggaaacctat tcccacatac aagctgacaa gcacaggaaa acctgcttca tcaatcgtat
    13441 acgtattttt agaaataact gatgttccta aaagaatcat aactataaga taaaataaca
    13501 taaatgtact tacattctct ggaagaaaga aaaaccatct ttcttttggc aaaacaagta
    13561 acactgcacc aacggcggat aaaactcctt caaaacttaa gagtgtcaat cctttcatcc
    13621 ggaacagctc gtaaactcct actaccgcta agattgctgc tgtaagctca accgctatgc
    13681 ctccgatcca tataatcgga ataaacaaga ctagcgccac agctgctgtg atcacacgtt
    13741 gtttcatgac tgatcccctt tctcatcttc tgtttttttt acaccgccga atcgacggtc
    13801 tcgattttga taagaggcga ttgcttcttc aagatgcgca ccatcaaaat caggccaaag
    13861 cgcttttgta aaatacagct cactatatgc gatctgccat aacagaaaat tactaattcg
    13921 ctcttctcca cttgtacgga tcatcagctc aggatcttgt aactctttag gcaagaaacc
    13981 tgtcattaaa tgcttagcaa tcaattcatc atcgatttct tctgcgtgta tttctccttt
    14041 tgctacttct tcggcaattt cttggacagc agtcacgatc tctgctctac ttccataatt
    14101 caaagcaaaa ttcaatacca ttccagtatt gttttttgtt tgctcaattg cacgtcttac
    14161 agcatcttgc gtatgttctg gcaatacgtt ttcataaccc atgacatgaa ctttgacatt
    14221 ttctttaatc aattctggta cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa
    14281 acttacttca tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac
    14341 tttcactcca agccttgatg ctttttttgt gactttcttt actgtttcca tgccttcttt
    14401 atggccagca actcgtggca aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat
    14461 gatggcaatg tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc ttttttcttg
    14521 aatatatttg tttttttgcg gaaaaaaacg taacattctt tttcctccta tacttaaaag
    14581 attcccacta tcttaagtat tatagcaata tctaactgtt tttcctatga aatcattgtt
    14641 tttttattta aaaactgaat aattttttct ttttttaatt tttaaataaa agaaatataa
    14701 ataaaaacag aggaagcgga atgatccgcc cctctgtttt tatgatttta ttaaacttcc
    14761 aataattctt tttctttatc agcagcgatc ttatctactt ctttaatgct gttatctgtc
    14821 aatgtttgta cgtctttttc taatccgcgc aaatcatctt cggtaatatc accatttttt
    14881 tgttgttttt tatattcatc gatcgcatca cgacggatgt tacgaatcgc gatttttgca
    14941 ttttctgctt cttttttcac atctttagct aattcttttc tgcgttcttc tgtcagttgc
    15001 gggataacta agcgaatgac atttccatca ttggttggac tgattccgat atcacttgcc
    15061 ataatagctt tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca
    15121 cgtgcttctg gaatttggat tgatgccatt tggttcaatg gtgttggcgc accgtagtag
    15181 ttcacagtaa tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt
    15241 tcacgttgaa gattttgtgc tgcttttttc attttttctt ttgcttctgt cataattgca
    15301 tctgccattt ttatttcccc cttacagttg ttccgatatt ttcgcctaag attgcacggc
    15361 gaatatttcc gtgttcattt aaattgaaga caagcagtgg aatatcatta tccatgctta
    15421 gtgaacttgc agttgaatcc atgacttgta atcctttggc aataacttcc agatgagtca
    15481 gttcttcaaa ttttactgca tttgcatcta ctttcggatc agcagaataa acaccgtcta
    15541 cattgttttt agccatcagg ataacatctg cgccgatttc tgccgctcgt aatgctgctg
    15601 ttgtgtctgt cgagaagtac gggttgccgg taccgcctgc aaagataacg atccgtcctt
    15661 tttctaaatg acgttctgct ttacggcgaa tatatggctc tgcgatttga cgcatttcaa
    15721 tcgaagtttg cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt aaagctaagg
    15781 cattcataac tgtagctaac atacccatgt aatcagcttg agcacgttcc atgcccatat
    15841 gtgctccgat ttgtccgcgc cagatatttc cgccacccac aacgattgcc atttcaacgc
    15901 ccagctcgtg aacttctttg atttcttgga tgatttcttt gattactggt ggtttgattc
    15961 caaatcctgc atccccagct aaagcttctc cactcaattt taagactaca cgctgatatt
    16021 tcggtgtgac catgttcatt cctccaatat tttctattgc cattttacca tacttagatg
    16081 atttcgacac tctaagactg gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc
    16141 gtgcgttccc gtctgttatt caacagaatt attttttcac ttggctcata acttcttcta
    16201 caaagttatc ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg aatgttttaa
    16261 cagttgcgcc ttttgatgca acgtattttt caacagtcat atcaggatct ttaacgaatg
    16321 gttggtccac caaagcgatt tctgctttga atttcttcaa gcgtccttct accatttttt
    16381 caacgatatt cgctggtttg ccttcgttca atgcttgttc tgtaagaaca gttttttcat
    16441 gttctaattc agcttctgga atttgtgatt cgtttacata acgaggattg atagctgcta
    16501 cgtgcatagc tacgtctctt gcaactgttt cgtcagttgt tccatctaga acagtcaata
    16561 cggcaatacg tccgcccata tgcaagtatc caccaaatgc agcattgtcg tctttttcta
    16621 caacttcaaa gcgacggaag ctgatttttt ctccgataac ttgagttgct tcgatcaagt
    16681 cagattcaat tgtaccttta tctgttttga ttttcattgc agcttccatg tctgctggtt
    16741 tgttttcagc aacaagttct gcaatttctt ttactaaatc ttggaacatt tcgtttttag
    16801 aaacaaagtc tgtttctgag tttacttcaa cgattgctgc agtgttacct ttcacagcaa
    16861 ctgaagccaa tccttcagct gcaatgcggt catttttctt cgcagccttt gccattcctt
    16921 tttcacgtaa aagatcaact gctttttcca tgtcgccttc tacttcaact aatgcttttt
    16981 tcgcatccat cattcctacg ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa
    17041 cgtctgccat gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg
    17101 agagtgaggc ttgtcgccca gcctttgaaa cagctccatc attcaaaatt attctgctga
    17161 agaattgtcg ccttcaacaa catctacgat ttcttcgatt gatgttgctg tgttttcttc
    17221 tgtgaaatct tcttcaacta cttgatcttc accttggttt ccttcgatga aagcatcagc
    17281 catttttgaa gtaatcaatt taacggcacg aattgcatcg tcgtttgaag ggattactac
    17341 gtcgatttca tctggatcgc agtttgtatc aaccatagct acgattggga tatttaattt
    17401 gtgtgcttct tgaacagcaa tacgttcttt acgtgggtca acgatgtaca ttacatctgg
    17461 gattcttggc atatcagcga taccgcctaa gaatttttca agacgttcac gttctttgtt
    17521 caaaccagca acttcttttt taggtagaac ttcaaaagtt ccatcttctt ccattgcatt
    17581 gatttgtttc aaacggctga tacgtttttg gatagtatcc cagtttgtca atgttccgcc
    17641 taaccaacga tggttaacga agtattgtcc tgcacgagtt gcttcttctt tgattgcttc
    17701 ttgtgcttgt ttttttgtac ctacgaataa tgctacgccg ccttcttcag caacgctctt
    17761 catgtaatca taagctgcat ctactaattt aactgttttt tgcaagtcaa tgatgtagat
    17821 tccgtttctt tctgtgaaga tatatttctt catttttgga ttccagcgac gagtttggtg
    17881 accaaagtgt acgccggctt ctagtaattg tttcatagaa attactgcca tgtttgtttc
    17941 ctccgatttg gttttatttt tcctcttctt atcttctacc ttgccggcaa actttttcaa
    18001 gcacctaccg acaatccaat aagaatgtgg atttgctgat gcataaagca ccctcagcta
    18061 ttatacagac aaaagatccg tatcgcaaga gcaaatatac ttttctgctc aacattcaat
    18121 agtttacctg aaagataaaa agaattcaag aaaaacagta gatttctctt tgtttcttta
    18181 ttatcctttt tccataaaat aattttaggt ataaaagacg aatattattg ggcaatcctt
    18241 cttttttttg ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc
    18301 tcaaagacat ttgctgatta aacaagtcat tgaagaattc ccgatccgta cgcaagaaga
    18361 actattaaac aaacttgggc attatggcgt taccgccacg caggcaacga tttctcgaga
    18421 tatccgtgat ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt
    18481 gtttcgcgaa aaaggaactc ctgagactag gactgaggaa gaggatcggc tggttcaaat
    18541 gattgaagac attgtattga aagtcgaacg agtccacttt ttgacgatcg tcaatacctt
    18601 gccagacaat gctcatttat ttgcatctgt tttagatgag atcaaacctc cctctattgt
    18661 cagtacaatt gctggattcg atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa
    18721 acgagtagaa gaatttcttc accatccaac tgaagtatca cttttttaat cagagcacgt
    18781 attcctaata tggaatgcgt gtttttttat tttcaatatt ttccttatta aataaacctt
    18841 tacactcttt acaaaaatac atcatttttt attcaatttt ttcaacacct tgattgaatc
    18901 acaataatca tcatttatat tggatgttaa caagagcagc ttcttgctct ttgtgtattt
    18961 caaaaaagaa atgaaaggaa atcatatgta taaattatta actcctggac cattgacgac
    19021 ttctgaaaca gtgaaaaatg ctatgctgat cgatcattgt acttgggatc aagaatacaa
    19081 agaaattaca caatggataa gaaaaaaatt attggatctt gctcaagtat cggaaggaat
    19141 atacacaacg gtcttaatgc aaggtagcgg ctcctttggg gtagaatcgg tattaagttc
    19201 tgtgatcagg ccagaagata ctgtactgat ttgttcaaac ggcgcatatg gacaacgaat
    19261 gatcaacatg tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat cggaacatca
    19321 aatcctccaa gctcatgtga ttacacagct gattgaagag aatccctcta tctccgcact
    19381 agcaatggtc catagcgaga ctacttccgg tattttgaat aatttggaag agattgctgc
    19441 tgtcacacaa gaaaaagatc ttttgtttat agtggacgcg atgtcaagct ttggtgggat
    19501 accgattcgt gtagccgaac tagggattga ttttttgatt tccagctcaa acaaatgtat
    19561 tcaaggtgtt ccaggctttt cttttgtact tgctaataaa gcggaactag aaaagcgaaa
    19621 aaatgtgagt cgaagtttgt cacttgatct atatgaccag taccgcgtga tggaaaacga
    19681 gaatggcaaa tggcgtttca cttcccctac ccatacggtt ttggcttttc aacaagcgct
    19741 gaaggaacta gaggcagaag gaggaatcga aactcgatat tcacgttatc tcgccaataa
    19801 tcatgtacta cgacaaaaaa tgacagagat ggggttccag cctttcattc aagaatatca
    19861 ggggccattc atcacctctt ttctttaccc taaggactgg cgatttgatt ttaatcattt
    19921 ttatcagtat ttaaagcaaa acggatttgt gatctatccc gggaaaatca gcgatcatga
    19981 atgttttcgt atcggaaata ttggcgagat ctatccggaa gatattttgg aactgacagc
    20041 aattatcaaa gactataagg agaacgttaa tgattaaaac tgtaattttt gattgggcag
    20101 gaacaactgt ggactttggc tgtatggctc ctgtccacgc ttttagaaat gcttttctag
    20161 aaaaaggaat ccaactgact gacaaagaaa tccgcgaacc gatgggaaaa ttaaaatggg
    20221 atcatatcca acagcttctt tccctccctt ctgtcaaaaa acaatggata gctatttatg
    20281 gacaactacc tcaggaaaaa gaggtggagg aattgtatca acaatttgaa cattatctgt
    20341 ttaaagattt ggtcaagcat tctaccttga aacccgacac attggaaacg atcgaacggt
    20401 tgagaaacgc tgggatcaat attggttcca cgactggatt tactgctcct atgatgacaa
    20461 tcgtagcaga aaccgcaaaa aaagaagggt attcaccaga cttcatggca acttctgaag
    20521 acgtcgaagg gtatggtcga ccgtttcctt atatgatcca taaaaacatg cagtacttca
    20581 aaaacaagtc ggttgatgaa gtaatcaaag tcggagatac ggtctcagat atccaggaag
    20641 ggaaaaatgc cggagtgttg acagtaggtg taattgaagg tagttcgcta atggggattt
    20701 cttatgatga gtaccaaaaa ttagagatgg ggaaaaaaga tcgtttaaaa cagtctgttg
    20761 ctcttagatt ccaacaagct ggcgctgata tttgtgtaaa caatttaaaa gagttaacag
    20821 aatacatttt gtacgctgac aagaatccag aatgactttt atacaaaatc agaggtcgtg
    20881 aaaaagctgt actgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcatatttt
    20941 tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta ttcgaaaaaa
    21001 aaagggagct gtgacacgac ttttgtcaca gtccccttat ttacaaacaa acagtgttta
    21061 agagacaacc tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa
    21121 acgataaaat ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct
    21181 gatttcacat tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg
    21241 tcttctttca ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca
    21301 tcatcagcta aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt
    21361 gcatgtttcg caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg
    21421 tatttttgaa ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct
    21481 gtttctgaag ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat
    21541 tgcacataag gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat
    21601 gtattcagtc gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta
    21661 gctacagcat ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat
    21721 tggattgctt catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat
    21781 tttttaccac cttgcgtgat caaaccaaat ggaatatggc gcatatcgtc agaccagtca
    21841 aagcccattt ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct
    21901 actacatata gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag
    21961 tcacgtgtaa tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca
    22021 taagcagata ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct
    22081 aaaagtttga cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg
    22141 aaacgtactt ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac
    22201 cattgccata attgaatagc ctcttcatcg ccttcttcca gacgtttgaa ccaagcacga
    22261 gcttcttcat ttaattctgg ttctgtttca gctacttcat ggaattggac atataaacga
    22321 agcaattcat tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg
    22381 acgatcagtt ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga
    22441 taaccgattt tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt
    22501 cccattgaga ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga
    22561 ttcccgatat tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt
    22621 ttgctgataa gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt
    22681 tcaaagttgg cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt
    22741 cggtaaactt ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt
    22801 ggattttcta gtaattgttc gacttgttcc agtgttaaat catctttgac agcgtcatag
    22861 acagcttttg cgacaagatc tttattattc atctttattc ctcctaaagt gctcccttga
    22921 ttgttaaaca aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag
    22981 atttcccgcg gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta
    23041 gacgaccgtc tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg
    23101 gcttccacca cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa
    23161 atcttagtct tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt
    23221 ttctgataaa aaaacaatcc agatggccta aaggagaaga aataccacct ggatagctat
    23281 aaatcatttc gtaaatacga aagacttaac aaattaatca gctacgataa tcgttccttc
    23341 ttcagaagct aaaagattct cgatattttc taaagaagtg atgattgcct tagcgtttgg
    23401 ttttgcttca acaaatgcaa tagctgcctc tactttcgga agcatgcttc ctggagcaaa
    23461 ttgcttttca tcaatgtatt gtttcatttc tgaaacagta acagtttcta atttcttttg
    23521 atctggtttt tggtaattga catacacatt gtctactcct gtcaatacga tcagcaaatc
    23581 cgcatcgatg atttcggcta atttttctga agcaaaatct ttatcgatta cggcttctcg
    23641 tccttctaat ccagtagctg tttctacaac aggaatccca ccgccaccaa cagagactgt
    23701 gataactcct tgatcgacca gcgtttcgat gacacgtgct tctttaatcg agatgggttt
    23761 aggggaagcg actacttttc tccatccacg tccagcatct tctttgaatg tcacagtgga
    23821 gtcagcattc atttgttctt tggcttcttc ttccgtgtag aatggaccga ctggtttact
    23881 tggatttttg aatgaaggat cattttcatc cacaataact tgagtgacta aagaaaccac
    23941 atctttatcg atcccttttt ccttcagtac ttcgcccatg gcattttgta accagtaacc
    24001 gatagatcct tctgtcattg caacacatgt atctaaaggc attgccggtg ttttctcaga
    24061 atcagcagct tgctgctgaa tcaataggtt accgacttgc ggaccatttc cgtgggagat
    24121 gatcaattca tctccttgtt cgataaattt cactaaatac tttgctgttt ccattaacgc
    24181 ttcttgttgg gcttttgcac ttgcatccgt agacaagatt gcattacccc ctaaagcgac
    24241 aactactttt cgtttggcca tttcattcgt cctttcttca gttctaccga attttctaga
    24301 taacaaaacg agccagcgtt ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga
    24361 tgaatgtggt tatgcttaaa cgcgagggat aaacaagtta cctaatgttg ctgccatgat
    24421 cgctttgatt gagtgcatac gattttctgc ttggtcaaat tgacgagcat atttgctgcg
    24481 gaagacttca tctgttactt ccatttcagt gatgccaaag cgttctttca tttgttcacc
    24541 gtaaacagtg tttgtatcat ggaaagctgg caagcagtgc aagaagatca aacgatctgt
    24601 gttgtgtgtt ttttctacca tttccatatt gatttggtaa ggtttcaaca atttgatacg
    24661 ttcttcgaat ttatcttctt cacccattga tacccataca tcagaataaa ggacgtctgc
    24721 tccgtctaca cctttatcaa catcgtcggt aatcatcaat tgtgcaccgg atttttcagc
    24781 aaagccttct gccattttga cgatttcttc ttcaggttgt aattcttttg gtgcaacgat
    24841 acgcatattc gctcctaaaa ttgcacctgt gactaataaa gagttagcca tattgtttcg
    24901 accatctccg cagtaagcaa cagtgatacc ttctactgtt ccaaagtttt cttggatagt
    24961 taaaaagtca gcgatcattt gtgttggatg ccattcatca gtcaagccat tccaaactgg
    25021 aacaccagag aattctgcta gttcctctac cattttttga ctgaatccac ggaattcgat
    25081 tccatcaaac atacgaccta gtactttagc tgtatcttct gtggattctt ttttacctaa
    25141 ttggatatca tttgcaccta gatattcagg atgtgcacct agatcgatcg ctgcagtagt
    25201 aaatgcagaa cgagtacgtg tagatgtttt ttcaaataaa agcgcaatat tttttccttc
    25261 tagatagtga tgtgggatac cacgtttttt caagtctttc aagtgttctg aaaaatcgat
    25321 caaatattgt aattcttctt ttgtaaagtc tttttctgct aataagcttc ttccttggaa
    25381 tactgattct ttcatattaa acattctcct ttagattgtt tttatttaag attaaagagg
    25441 aaatcccgac ggcagctgac agtaagaccg ccgtcaggaa acaaattact tcaagtcttc
    25501 acggacaagt ggttggctca tgcagcgtgg accaccacgg cctcgtgaca attcacttga
    25561 attgatttct aacactttga tgccatagct acgaagcaat tcgttcgata cataatttcg
    25621 gttataagtg actacaacac ctggagcgat tgccaatgtg tttgatccat cattccattg
    25681 ttctcgaggt gcaacgatct catctccatt accagttggg atcagtacaa gatcatctaa
    25741 tccaagagca gctttcatcg tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc
    25801 accatctggt tcgattgtaa agacatccat cttaccgtct ttactttgaa ttgctggatg
    25861 gatagtgaat ttatcgtgat ctaccattgt aaatacggta tccaaatgca tcattgcacg
    25921 gttgtttgga attttgattg ctaacacttt ttcaaatcct gaattttttg cgaataggtg
    25981 gcgtgctaat ttttctaaag ctttcgcatt agttcgttga gaaatcccaa cagctactac
    26041 tttatcgctt aagatcaatt cgtctccgcc ttcgatatga tctggatttt cacgatccaa
    26101 ccatacttcg attcctttat ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa
    26161 catcgattca cgacgacgtg cttcaaaagt catcttattg actgtcatcc catttcccat
    26221 tgatgcggaa gggtcgcgtg taaagtacaa gtttggcatt gggtccatgt aaaatggata
    26281 gtcatcgtct gctgacagat cgtatagact gctagaacgt acttcaatat cttttgtacg
    26341 aaccccagcc atgattttat ctaccatttc ttgcgtttcc attgatagaa ggaactcatg
    26401 aagaccctct cttacagcat tcgatgcaat atgagattca tccaacattt tattcaagaa
    26461 ttgttctttg acattgcctg cgtcaatcgc ttctgccgct aatttttcca agtaaagagt
    26521 ctcgacgcct tcattttgca atgtctttgc aaagttgtca tgttcttctt gagcaatcgg
    26581 aagatatgga atatcatcga acaacagacg atccataata tctggtgtca aattttccac
    26641 ttcttgtcca ggccttttca gtaaaactgt ctttaatttc cctatttcag agaaaacgtg
    26701 aataggttta tccatgttgt gttgcctcct tctttttgat tacatgtata ggatatcctc
    26761 cttttgtaat cactttcata attttgtctc attcaaaatg agacattcct cacattaata
    26821 ttcgataatt aaataattaa acgtttttct atttttaagc gtaatttatt cattttttat
    26881 tctgtataca ctttcaaaaa aatatttatt cattgaaaaa aattttttga ataaattaaa
    26941 aaaataaatt atattagcgt tgttagcgtt tactatatgt attattgttg ttttttatac
    27001 acaaatcatt cgcttcaaaa caaattattc aataccattg tatatttttt acataataac
    27061 aaattatatt tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa aactttacaa
    27121 attctttaaa aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg
    27181 tgttaataaa atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt
    27241 ctcggatgaa gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg
    27301 acagatcctt ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact
    27361 cgttcgttta gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac
    27421 aaataacctt tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca
    27481 agcgatgact gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg
    27541 aaaaaatccc gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca
    27601 tgaattgcgt atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt
    27661 attcattttg aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta
    27721 tccgatcacc ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca
    27781 agtaatcaaa caactacgtg attccggact gttagattat gacaaaaagc gttttacttt
    27841 ctgtaagaca gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac
    27901 cctgatattt atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca
    27961 gttttagatc actgggcaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt
    28021 cactgtttgc aaaaaatact gatagattaa ctagaggaag cgtcttgtcc tcatccgacc
    28081 tagattattc gatattcctc gacaataaac gattaatcag tgagggcaat cagaggaacg
    28141 gataatgatg aataagctgt aaaaaatagg gctttgacaa aagcgtgtca cagcccttat
    28201 ttcgattaaa cggtgtgtac ctgtcaactc ttcggtcaca agtcataatg ttcataaaca
    28261 tgaggagctg ttttttgaaa ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa
    28321 ctcttgatta aacggcgttc aaaagcttgc atacgcggta ataagaacag ccaggtcaaa
    28381 aacatgagaa gccgtttttc ctgggctgtt cttattactt gatgcagggc agatttttca
    28441 caacctcttt attcagataa aaccaagctt ggatcagcat tcaactgata gctagcaaaa
    28501 tttctttttt gcatttccac atgtgctgct gctccaatca ttgcagcatt atctccacaa
    28561 agtcgaagag ggggaatcac tagttctacc tcagggagtt tagttgccaa agcatcctgc
    28621 aatccttcac gcaacccttg attggccgct acacctccag ctacaatcag ttgcttgact
    28681 ggataatttt gacaagctcg aagtgtttta gtaatcaata catcgatcac acttgcttga
    28741 aagctagctg ctaaatcatt tttgtctaga tcttctccac gctgttgtgc attatgcacc
    28801 aaattgataa atgcactttt taatccgcta aaactgaaat cataattatc ttcatggatc
    28861 atcgctcgtg gaaaatggta attatctttt ccctgatgag ctagctgatc gatttctttt
    28921 ccgcttgggt aagaaagacc taatactctt ccaactttgt cataagcttc tcctgctgcg
    28981 tcatctcttg tctccccaat gatttcatat gatccatctt cttgcatata gactaattct
    29041 gtatgtcctc cgctaacaag aagtgccatc aatggaaact ggaatggttt aaccagacgg
    29101 gctgcataga tatgccctgc catatggttt acaggaataa gcggcaattg gtgtgcccaa
    29161 gcaaaagcct tagctgcaga aataccaatc agcaacgagc cgacaagtcc tggtccataa
    29221 gtgacagcga ctgccgataa atcttctgcc gacacaccag cttctaccag tgcatcttcc
    29281 aagcataagg tgatttgttc tacgtgatgg cggctagcta cttcaggaac tacgccacca
    29341 aaacgtttat gactgtttat ttgagaagca acgatgttag ataagatctc tgttccattt
    29401 ctaacgaccg ctacactcgt ttcatcacaa cttgattcaa tcgccagaat caattcttca
    29461 ttcatttcat cgtctccgtt tttaactttc tgctcataat gatcgcatct tccgtcggat
    29521 tgcgataata tgatttccgt ttacctaata tacgaaactt ttctgattca taaagttttc
    29581 ttgctgcttc attagataga cgtacttcta aaaaaacatg cagcgaatct tttatttgtt
    29641 ccgtaatcag taattgtaac aaatgccgtg catgcccttt tccttgttct gaaactgcaa
    29701 cagcaatatt tgtgatctcg acttcatcta ttactttcga gtatccaata aagccacaga
    29761 tctggttatc ttcaactaaa agcagataat cagagtgagg ctgttggatg tctgccaaaa
    29821 actgcatttt cgtccaaggt gaaccatgtg gataagcatt ttcgctaatc atccatacgc
    29881 attctgccaa gtacgcttca tcaaaatctt tttttctgca aatcaactaa tctcactcat
    29941 tttctaaaag ttttatcata tccaacgcat cttcttttgt ttcagtgtaa tatctttttt
    30001 tgatcgttgt tgcttgaaaa cctaacttcc gataaagtct ttgtgcatct tgattactca
    30061 tacgcacttc taatgacatc gtctcacagc gattcattat agcaaagttc tcgatttcgt
    30121 caataagcag cgaaccgatt cgtttacgct gatatgccgg tcgaacggca atattcgtga
    30181 tatggcaatc taaaccgaac gctcttgaac cgataaaacc aatcattctt ccattatcaa
    30241 agatacctag atatagatga ggaagcggtg acttcaattc tgatataaag gcactttttg
    30301 tccatggcaa ttcgccatta tagacttctc gttcgacatc cagcagctct ttgatatctt
    30361 ctgtagtcaa ttctcttagt acgtaacgac gatcgatttg ttcgattgtt ttttctgtat
    30421 aaatcatttt atcttgaaaa aagcttctgg catacgctct aaattttttc aacataactt
    30481 tcgtctccag gtgtgtgcgt ctccaaccat ttttcttctg cttctacccg ttttaaatag
    30541 cgaggaagaa aagcttgttt ctctgcaact ggttcagcta aagcacctaa ttgagccaca
    30601 gttacaccac ttggaatatc ccatgctgga actgtattaa tcatcgcact aggcaatatt
    30661 tcctttattt catagaagaa tttgtgacaa tcactcccga cgaaataaat agcttcattc
    30721 agcttttgta gttgtgtgaa caaatcagaa gcagaaatat gtacatcttc aagtacaatt
    30781 tctaatctat tttctttcca gcggtatgcc ccagcgtata cattttttct tcgagcatca
    30841 aacatgggca caataatttt atccacaccg atgcaattag ccgcgatggt tttcagacta
    30901 gatactccta ctagctcttt atctaatgta taagcaatcg tttttgctgt cgttacacct
    30961 atacgaagcc ctgtataaga accgggacca tcagaaaccg cgattcggtc aatgtctttt
    31021 ggacttattt gaagatctgc aaacaattgg tcgattgcag gcatcagtgt tacactgtgg
    31081 ttgcgtttga tatttgtttg tatttgtcca agaatttgtt cgtcttctac cacaccgatc
    31141 gccaaagttt ggtttgcagt atctattcct aatgtaatca tatatttgat cctttctgct
    31201 atgacattct cgccttccat tgtagcatac ttttttattc tgaggatgaa aataccaaag
    31261 gcaaaactag tacaatgata catgattatc tgctggcggg caaatttt
//
LOCUS       DACCQI010000017        31278 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCQI010000017 DACCQI010000000
VERSION     DACCQI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.017"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(87..464)
                     /locus_tag="IVO76_000619"
     CDS             complement(87..464)
                     /locus_tag="IVO76_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428606.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            720..1448
                     /locus_tag="IVO76_000620"
     CDS             720..1448
                     /locus_tag="IVO76_000620"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7428607.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1448..1702
                     /gene="purS"
                     /locus_tag="IVO76_000621"
     CDS             1448..1702
                     /gene="purS"
                     /locus_tag="IVO76_000621"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7428608.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1704..2375
                     /gene="purQ"
                     /locus_tag="IVO76_000622"
     CDS             1704..2375
                     /gene="purQ"
                     /locus_tag="IVO76_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7428609.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2376..4598
                     /gene="purL"
                     /locus_tag="IVO76_000623"
     CDS             2376..4598
                     /gene="purL"
                     /locus_tag="IVO76_000623"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7428610.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4583..6022
                     /locus_tag="IVO76_000624"
     CDS             4583..6022
                     /locus_tag="IVO76_000624"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7428611.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6054..7097
                     /locus_tag="IVO76_000625"
     CDS             6054..7097
                     /locus_tag="IVO76_000625"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7428612.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7094..7672
                     /gene="purN"
                     /locus_tag="IVO76_000626"
     CDS             7094..7672
                     /gene="purN"
                     /locus_tag="IVO76_000626"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7428613.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7669..9204
                     /gene="purH"
                     /locus_tag="IVO76_000627"
     CDS             7669..9204
                     /gene="purH"
                     /locus_tag="IVO76_000627"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7428614.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9220..10494
                     /gene="purD"
                     /locus_tag="IVO76_000628"
     CDS             9220..10494
                     /gene="purD"
                     /locus_tag="IVO76_000628"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7428615.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10616..11530
                     /locus_tag="IVO76_000629"
     CDS             10616..11530
                     /locus_tag="IVO76_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7428616.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11663..13228)
                     /locus_tag="IVO76_000630"
     CDS             complement(11663..13228)
                     /locus_tag="IVO76_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7428617.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13221..14672)
                     /locus_tag="IVO76_000631"
     CDS             complement(13221..14672)
                     /locus_tag="IVO76_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7428618.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14686..15702)
                     /locus_tag="IVO76_000632"
     CDS             complement(14686..15702)
                     /locus_tag="IVO76_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7428619.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15922..17010
                     /locus_tag="IVO76_000633"
     CDS             15922..17010
                     /locus_tag="IVO76_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7428620.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17064..18590
                     /locus_tag="IVO76_000634"
     CDS             17064..18590
                     /locus_tag="IVO76_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7428621.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18592..19797
                     /locus_tag="IVO76_000635"
     CDS             18592..19797
                     /locus_tag="IVO76_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7428622.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20054..21031
                     /gene="guaC"
                     /locus_tag="IVO76_000636"
     CDS             20054..21031
                     /gene="guaC"
                     /locus_tag="IVO76_000636"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7428623.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21295..22719
                     /locus_tag="IVO76_000637"
     CDS             21295..22719
                     /locus_tag="IVO76_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7428624.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23180..24289
                     /gene="pdhA"
                     /locus_tag="IVO76_000638"
     CDS             23180..24289
                     /gene="pdhA"
                     /locus_tag="IVO76_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7428625.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24292..25269
                     /locus_tag="IVO76_000639"
     CDS             24292..25269
                     /locus_tag="IVO76_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7428626.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25294..26589
                     /locus_tag="IVO76_000640"
     CDS             25294..26589
                     /locus_tag="IVO76_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAP7428627.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26597..28003
                     /gene="lpdA"
                     /locus_tag="IVO76_000641"
     CDS             26597..28003
                     /gene="lpdA"
                     /locus_tag="IVO76_000641"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7428628.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28165..28440
                     /locus_tag="IVO76_000642"
     CDS             28165..28440
                     /locus_tag="IVO76_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7428629.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28446..29213
                     /locus_tag="IVO76_000643"
     CDS             28446..29213
                     /locus_tag="IVO76_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7428630.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29381..31210
                     /gene="typA"
                     /locus_tag="IVO76_000644"
     CDS             29381..31210
                     /gene="typA"
                     /locus_tag="IVO76_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7428631.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT        10230 a         5364 c         7024 g         8660 t
ORIGIN      
        1 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
       61 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
      121 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
      181 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
      241 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
      301 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
      361 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
      421 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
      481 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
      541 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
      601 tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
      661 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
      721 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
      781 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
      841 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
      901 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
      961 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
     1021 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
     1081 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
     1141 aacttgcaaa gccggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
     1201 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
     1261 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
     1321 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
     1381 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
     1441 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
     1501 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
     1561 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
     1621 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
     1681 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
     1741 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
     1801 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
     1861 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
     1921 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
     1981 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
     2041 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
     2101 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
     2161 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
     2221 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
     2281 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
     2341 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
     2401 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
     2461 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
     2521 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
     2581 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
     2641 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
     2701 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
     2761 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
     2821 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
     2881 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
     2941 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
     3001 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
     3061 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
     3121 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
     3181 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
     3241 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
     3301 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
     3361 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
     3421 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
     3481 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
     3541 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
     3601 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
     3661 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
     3721 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
     3781 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
     3841 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
     3901 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
     3961 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
     4021 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
     4081 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
     4141 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
     4201 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
     4261 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
     4321 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
     4381 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
     4441 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
     4501 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
     4561 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
     4621 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
     4681 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
     4741 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
     4801 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
     4861 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
     4921 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
     4981 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
     5041 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
     5101 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
     5161 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
     5221 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
     5281 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
     5341 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
     5401 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
     5461 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
     5521 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
     5581 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
     5641 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
     5701 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
     5761 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
     5821 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
     5881 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
     5941 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
     6001 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
     6061 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
     6121 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
     6181 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
     6241 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
     6301 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
     6361 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
     6421 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
     6481 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
     6541 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
     6601 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
     6661 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
     6721 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
     6781 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
     6841 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
     6901 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
     6961 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
     7021 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
     7081 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
     7141 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
     7201 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
     7261 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
     7321 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
     7381 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
     7441 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
     7501 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
     7561 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
     7621 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
     7681 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
     7741 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
     7801 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
     7861 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
     7921 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
     7981 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
     8041 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
     8101 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
     8161 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
     8221 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
     8281 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
     8341 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
     8401 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
     8461 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
     8521 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
     8581 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
     8641 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
     8701 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
     8761 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
     8821 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
     8881 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
     8941 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
     9001 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
     9061 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
     9121 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
     9181 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
     9241 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
     9301 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
     9361 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
     9421 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
     9481 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
     9541 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
     9601 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
     9661 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
     9721 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
     9781 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
     9841 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
     9901 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
     9961 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    10021 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    10081 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    10141 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    10201 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    10261 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    10321 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    10381 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    10441 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    10501 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    10561 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    10621 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    10681 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    10741 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    10801 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    10861 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    10921 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    10981 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    11041 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    11101 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    11161 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    11221 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    11281 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    11341 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    11401 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    11461 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    11521 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    11581 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    11641 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    11701 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    11761 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    11821 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    11881 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    11941 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    12001 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    12061 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    12121 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    12181 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    12241 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    12301 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    12361 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    12421 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    12481 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    12541 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    12601 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    12661 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    12721 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    12781 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    12841 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    12901 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    12961 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    13021 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    13081 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    13141 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    13201 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    13261 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    13321 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    13381 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    13441 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgtg
    13501 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    13561 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    13621 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    13681 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    13741 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    13801 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    13861 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    13921 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    13981 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    14041 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    14101 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    14161 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    14221 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    14281 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    14341 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    14401 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    14461 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    14521 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    14581 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    14641 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    14701 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    14761 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    14821 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    14881 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    14941 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    15001 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    15061 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    15121 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    15181 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    15241 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    15301 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    15361 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    15421 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    15481 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    15541 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    15601 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    15661 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    15721 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    15781 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    15841 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    15901 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    15961 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    16021 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    16081 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    16141 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    16201 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    16261 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    16321 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    16381 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    16441 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    16501 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    16561 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    16621 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    16681 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    16741 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    16801 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    16861 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    16921 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    16981 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    17041 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    17101 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    17161 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    17221 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    17281 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    17341 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    17401 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    17461 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    17521 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    17581 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    17641 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    17701 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    17761 agcatattat tcgcggaatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    17821 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    17881 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    17941 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    18001 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    18061 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    18121 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    18181 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    18241 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    18301 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    18361 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    18421 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    18481 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    18541 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    18601 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    18661 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    18721 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    18781 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    18841 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    18901 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    18961 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    19021 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    19081 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    19141 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    19201 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    19261 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    19321 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    19381 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    19441 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    19501 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    19561 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    19621 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    19681 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    19741 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    19801 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    19861 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    19921 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    19981 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    20041 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    20101 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    20161 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    20221 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    20281 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    20341 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    20401 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    20461 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    20521 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    20581 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    20641 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    20701 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    20761 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    20821 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    20881 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    20941 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    21001 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    21061 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    21121 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    21181 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    21241 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    21301 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    21361 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    21421 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    21481 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    21541 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    21601 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    21661 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    21721 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    21781 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    21841 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    21901 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    21961 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    22021 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    22081 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    22141 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    22201 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    22261 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    22321 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    22381 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    22441 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    22501 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    22561 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    22621 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    22681 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    22741 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    22801 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    22861 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatagttg
    22921 taagtttgat tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg
    22981 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    23041 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    23101 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    23161 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    23221 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    23281 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    23341 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    23401 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    23461 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    23521 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    23581 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    23641 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    23701 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    23761 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    23821 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    23881 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    23941 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    24001 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    24061 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    24121 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    24181 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    24241 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    24301 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    24361 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    24421 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    24481 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    24541 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    24601 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    24661 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    24721 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    24781 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    24841 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    24901 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    24961 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    25021 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    25081 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    25141 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    25201 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    25261 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa attgccggat
    25321 atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg
    25381 atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga agaaattccg
    25441 tcacctgtca cagggacagt taaaaacgtg attgttccag aaggcactgt agcaaatgtt
    25501 ggggatgtat tagtagaaat cgatgctcct ggacacaata gtgcaccgtc aactagtgcg
    25561 ccatcagctg aagcaccaaa agaaaaagtg gaaacatcag gttcagcaag tgtcgttgaa
    25621 gctgccgatc caaataaacg tgtattggct atgccatctg ttcgtcaatt tgcccgtgaa
    25681 aaagatgtag atatctcaca agtaactgca actggaaaag gtggcagagt aacaaaagag
    25741 gacatcgaaa acttcttggc aggtggtcca agcagtgcac cagcgaaatc agaagcgcca
    25801 gaagcggcag caccgaaaga agcagctcct gcagcagaaa gcaaaccagc tgcaccagca
    25861 aaaccattca agtccaactt aggagacttg gaagaacgtg tagcaatgac gccaacacgt
    25921 aaagctatcg ctaaagcaat ggtcaacagt aagcatacag ctccacacgt gacattgcat
    25981 gatgaagtag aagtgtctaa actatgggat aatcggaaac gatttaaaga agttgctgct
    26041 gcaaatggta cgaaacttac tttcttgcca tatgtcgtga aagctttaac agcgactgtt
    26101 aagaaatacc cagtattgaa tgcatctatc gacgatgcaa accaagaaat cgtttataaa
    26161 cattactaca atatcggtat tgcaactgat acggatcacg gactatatgt accaaatgtg
    26221 aaagatgcag accgcaaagg aatgtttgct attgcagacg aaatcaatga gaaagcaaaa
    26281 ttagctcatg acggcaaact atctgcagaa gatatgcgca atggaacaat tacgatcagc
    26341 aatatcggat ctgtaggtgg cggttggttt acaccagtca tcaactatcc agaagtagct
    26401 attttaggtg taggaacgat tgcacaacaa ccaatcgtaa atgctgaagg cgaaatcgtt
    26461 gtcggtcgtg tgatgaaact gtcattaagt ttcgatcacc gtatcgtaga tggagcaact
    26521 gcgcaacaag caatgaataa tatcaaacgt cttttagctg atccagagct attaatgatg
    26581 gaaggatgat aaacaaatgg tagttggaga ttttgctgta gaattagata cagttgtaat
    26641 tggtgcggga cctggtggct atgtagctgc catccgcgct gctgaaatgg gacaaaaagt
    26701 agcaatcatt gaaagagagt atattggcgg tgtttgtttg aatgtcggct gtattccttc
    26761 aaaagcactg atagctgcag gacaccatta tcaagagtca ctagattctg aaatgtttgg
    26821 agtaactagt gaaaacgtca aactagattt tgcaaaaaca caagaatgga aagaaaacaa
    26881 agttgtccat acgttgactt ctggtgttgg tttcctatta aaaaaacata aggttgaaac
    26941 aatcgaagga gaagcgttct tcgtagatga tcacacacta cgcgtcattc atccagattc
    27001 agctcaaact tattcgttca ataacgcaat catcgcaaca ggttcccgtc caatcgagat
    27061 tccaggattt aaattcggag gacgtgtgtt agattctact ggcggattag cattgaaaga
    27121 agtacctaag aaatttgtga tcatcggtgg cggtgtgatt ggtgccgaat tgggcggagc
    27181 gtacgcaaac ttaggagcag aagtaacgat tcttgaaggc tcaccacaaa ttttaccaac
    27241 ttacgaaaaa gatctagtaa aattagtaga agatgatttc aagaaaaaag gcgtaacagt
    27301 cgtaacgaat gcaatggcga aagaagcagt cgataacggt gacagcgtga cagtgaaata
    27361 tgcagtggac gggaaagaag aatccgttac tgctgattat gtgatggtca ctgtaggtcg
    27421 tcgtccgaat acagatgata tgggcttaga acaagcgggt gtcgaagtag gagaacgagg
    27481 attgatcacg gtggataaac aaggacgtac gaacgtacct aacatctatg ccatcggtga
    27541 tatcgttcca ggggcggcat tagcacacaa agccagttat gaagcaaaaa tcgctgctga
    27601 agcaatctct ggcaaaaaag tagctgtaga ttataaagca atgccggcag tagccttcac
    27661 tgatcctgaa ttggcttctg taggtatgac gatcaaagaa gcaaaagatg ctggtatcga
    27721 agcaaaagca tataaattcc cattttcagg gaacggtcgg gcattatcgc ttggtaaaac
    27781 agaaggattc atccgtctag ttacgacgat cgaagataac gtgcttattg gcgcgcaaat
    27841 cggtggagtc ggcgcaagtg atatggtctc tgaattagct ttagcaatcg aatcaggaat
    27901 gaatgctgaa gatatcgcct tgacgattca tcctcatcca tctcttggcg aaatcgtcat
    27961 ggatgcttcg gaattagcat taggtttacc aatccatatt tgatttgaat aatacaggcg
    28021 aaacgcaact gaaaaatcag ttgcgttttg ttttttagaa ttatcaataa tattattatg
    28081 taaacttaaa aatatgacaa cttcttggtt tattaccact tcctatgcta cactgaagag
    28141 gaatatgaaa taggagagtt gaagatgcaa gaatatcagt atccacttga tttagattgg
    28201 acaacagacg aaatggtcat tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa
    28261 aaagggataa acaatgaagc ttttttgaag acttatcagc agtttaaaac tgttgtcaaa
    28321 tccattggtg aggaaaaacg attgggacgg gaatttgaaa aagcttccgg ttattcttta
    28381 tatcgtacag tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga
    28441 gataaatgga acagatgatt gatcatatca agtcctggct tgaagaagct ggtgagaaaa
    28501 tcaaatcttc tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa
    28561 caaatgtcga tcgaaacatc cagaattttc taatcgaaaa aatccattcc tttgatccag
    28621 aggcgaaaat attggcagaa gaagaagggt gtaatcaatt atcggatctc gatggaagag
    28681 tctttatcat cgatcctatt gatggcacct tgaattttgt gattgaaggg gaaaattttt
    28741 gtattatgct cgctgtttat gaatccggcg ttggaaagct tggtttcatt tatgacgtga
    28801 tgagaaatga acttttttgg ggtggaaaag gaataggcgt gtataaaaat gatgagccat
    28861 tgcctagacc agcaaataaa tccttatcgg aaggcttact tggtgtgaat agttatcttt
    28921 tcggtcataa taaatatgga atccgagaaa ttgcggaaag cagcatgggg acacgtatgt
    28981 acggatgcgc tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt gcttatattt
    29041 caaaccttag cccttgggat tatgcagcgg gaaatgtctt attggaagag tttggtatgg
    29101 taacaagcgg tctttttggt actcctttga catttgaaaa aagggaatac tatttagcag
    29161 ggacttcttg tgcatatgca gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag
    29221 gtagaaatca ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg
    29281 aaaacatttt actatatttc atgaaaaaag tctgctataa ttagcagtcg agtaaaattt
    29341 gccctgtcat aaagaaccaa taaaaaagga gttagttaca ttgaattata gaaacgatat
    29401 ccgtaacgtc gcaattattg cccacgttga ccacggaaaa acaacattag ttgatgaact
    29461 attaaaacaa tcagatacat tagacgcaca tacgcaattg caagaacgtg cgatggactc
    29521 aaatgcgcta gaaaaagaac gtggtatcac gattttagcc aaaaacacag ctgtagacta
    29581 taaaggtatt cgcgtaaaca tcatggatac tccaggacac gcggacttcg gtggcgaagt
    29641 agaacggatc atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac
    29701 aatgcctcaa acacgttttg tattgaaaaa agcactggaa caacatatca caccaatcgt
    29761 tgtcgtaaac aagattgaca aaccttctgc tcgtcctgag catgtcgtag atgaagttct
    29821 tgaactattc atcgaattag gagcagatga tgatcagtta gactttcctg tgatttatgc
    29881 atctgcacta aatggtacgt caagcttgtc agatgatcca gcagatcaag aaccaaccat
    29941 ggcacctatt tttgatacaa ttattgaaaa aatccctgct cctgtcgata acagcgacga
    30001 accattacaa ttccaagttt cattacttga ctacaacgat tatgtaggtc gtatcggaat
    30061 cggacgtgta tttagaggaa caatcaaagt aggagaccaa gtagcattaa ttaaattaga
    30121 cggaacagtt aagaaattcc gtgtaacgaa attatttggt ttctttggac taaaacgtct
    30181 agagattcaa gaagcaaaag ctggagactt gattgctgtc tcaggtatgg aagatatctt
    30241 tgtcggtgaa acagttactc ctgtggatca tcaagatgcg ttacctatcc ttcacattga
    30301 cgaaccaaca ttgcaaatga ctttcttagt taataattca ccatttgccg gtcgagaagg
    30361 taaattcgta accgctcgta agattgaaga acgtttgatg gcagaacttc aaacagatgt
    30421 atccttacga gttgaaccaa caaactctcc agatgcatgg acagtttctg gtcgtggaga
    30481 attgcatttg tctatcttga ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc
    30541 tcgtccagaa gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt
    30601 gcaaatcgat acaccagaag aatatatggg tagtgttatc gagtccttga gcttacgtaa
    30661 aggtgaaatg caagatatgg tccatacagg aaacggacaa atccgtttga cattcttaac
    30721 acctgcccgc ggtttgattg gttattcaac tgaattcctt tcaatgacac gtggttacgg
    30781 aatcatgaac catacttttg accaatatct gccaatgctt cctggtcaaa ttggcggacg
    30841 tcaccaaggg gcgcttgttt cgatcgatac aggaaaagca acgacttatt ctatcatgag
    30901 tatcgaagaa cgcggtacag tcttcgtaga accaggtaca gaagtatatg aaggaatgat
    30961 tatcggggaa aacagccgtg ataatgattt gactgtcaac atcacgaaag caaaacaaat
    31021 gacaaacgta cggtctgcta ctaaagacca aacatctgtc atcaaaaaac caaaacaatt
    31081 gacgttggaa gaatctttgg aattcttgaa tgatgatgaa tattgcgaag taacaccaga
    31141 aagcattcgc ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa
    31201 gaaaaaataa tctgtaaaag gatgaagaga gaaaccagct tgttataaaa aatagctggt
    31261 ttcttttttt gtccaact
//
LOCUS       DACCQI010000018        31190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCQI010000018 DACCQI010000000
VERSION     DACCQI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.018"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..475)
                     /locus_tag="IVO76_000645"
     CDS             complement(<1..475)
                     /locus_tag="IVO76_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HAP7428632.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQG"
     gene            complement(565..1110)
                     /gene="hpt"
                     /locus_tag="IVO76_000646"
     CDS             complement(565..1110)
                     /gene="hpt"
                     /locus_tag="IVO76_000646"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7428633.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(1110..2555)
                     /gene="tilS"
                     /locus_tag="IVO76_000647"
     CDS             complement(1110..2555)
                     /gene="tilS"
                     /locus_tag="IVO76_000647"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7428634.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(2675..3145)
                     /locus_tag="IVO76_000648"
     CDS             complement(2675..3145)
                     /locus_tag="IVO76_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7428635.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(3191..3616)
                     /locus_tag="IVO76_000649"
     CDS             complement(3191..3616)
                     /locus_tag="IVO76_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7428636.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(3683..3952)
                     /locus_tag="IVO76_000650"
     CDS             complement(3683..3952)
                     /locus_tag="IVO76_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7428637.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(3963..5558)
                     /locus_tag="IVO76_000651"
     CDS             complement(3963..5558)
                     /locus_tag="IVO76_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7428638.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(5573..9094)
                     /gene="mfd"
                     /locus_tag="IVO76_000652"
     CDS             complement(5573..9094)
                     /gene="mfd"
                     /locus_tag="IVO76_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7428639.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(9111..9671)
                     /locus_tag="IVO76_000653"
     CDS             complement(9111..9671)
                     /locus_tag="IVO76_000653"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7428640.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            10024..10998
                     /locus_tag="IVO76_000654"
     CDS             10024..10998
                     /locus_tag="IVO76_000654"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7428641.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            11168..12568
                     /locus_tag="IVO76_000655"
     CDS             11168..12568
                     /locus_tag="IVO76_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7428642.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(12646..13368)
                     /locus_tag="IVO76_000656"
     CDS             complement(12646..13368)
                     /locus_tag="IVO76_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7428643.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(13447..13953)
                     /locus_tag="IVO76_000657"
     CDS             complement(13447..13953)
                     /locus_tag="IVO76_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7428644.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            14076..15875
                     /locus_tag="IVO76_000658"
     CDS             14076..15875
                     /locus_tag="IVO76_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7428645.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(15943..17517)
                     /locus_tag="IVO76_000659"
     CDS             complement(15943..17517)
                     /locus_tag="IVO76_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428646.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(17772..18281)
                     /gene="mreD"
                     /locus_tag="IVO76_000660"
     CDS             complement(17772..18281)
                     /gene="mreD"
                     /locus_tag="IVO76_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7428647.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(18285..19139)
                     /gene="mreC"
                     /locus_tag="IVO76_000661"
     CDS             complement(18285..19139)
                     /gene="mreC"
                     /locus_tag="IVO76_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7428648.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(19294..19932)
                     /locus_tag="IVO76_000662"
     CDS             complement(19294..19932)
                     /locus_tag="IVO76_000662"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7428649.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(19933..20583)
                     /locus_tag="IVO76_000663"
     CDS             complement(19933..20583)
                     /locus_tag="IVO76_000663"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7428650.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(20596..21495)
                     /gene="rsgA"
                     /locus_tag="IVO76_000664"
     CDS             complement(20596..21495)
                     /gene="rsgA"
                     /locus_tag="IVO76_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7428651.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(21660..23729)
                     /gene="pknB"
                     /locus_tag="IVO76_000665"
     CDS             complement(21660..23729)
                     /gene="pknB"
                     /locus_tag="IVO76_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7428652.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(23726..24466)
                     /locus_tag="IVO76_000666"
     CDS             complement(23726..24466)
                     /locus_tag="IVO76_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7428653.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(24479..25837)
                     /gene="rsmB"
                     /locus_tag="IVO76_000667"
     CDS             complement(24479..25837)
                     /gene="rsmB"
                     /locus_tag="IVO76_000667"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7428654.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(25837..26775)
                     /locus_tag="IVO76_000668"
     CDS             complement(25837..26775)
                     /locus_tag="IVO76_000668"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7428655.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(26768..27259)
                     /gene="def"
                     /locus_tag="IVO76_000669"
     CDS             complement(26768..27259)
                     /gene="def"
                     /locus_tag="IVO76_000669"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7428656.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(27274..29682)
                     /gene="priA"
                     /locus_tag="IVO76_000670"
     CDS             complement(27274..29682)
                     /gene="priA"
                     /locus_tag="IVO76_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7428657.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(29935..30240)
                     /locus_tag="IVO76_000671"
     CDS             complement(29935..30240)
                     /locus_tag="IVO76_000671"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7428658.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(30245..30859)
                     /gene="gmk"
                     /locus_tag="IVO76_000672"
     CDS             complement(30245..30859)
                     /gene="gmk"
                     /locus_tag="IVO76_000672"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7428659.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         8567 a         6732 c         5752 g        10139 t
ORIGIN      
        1 cgccttgttg tcccatcatc atgtaaaaga aaaagatgat gattacgatt ggcaagaagc
       61 taagcaatag ggaaacccat ataccgctgc ttgattcttc ttttactgtt gtagcaactt
      121 tgttttcttt tgccagttct gttacttggc tcaatgttgt atcatttggc aaaatgattg
      181 ttgtgaaatg ggtagtagtc gtatctgttg aacctaagat agctaaccca ccgctgttag
      241 cgacggtctg ctgttctttg tattcaccag tgatcttata tacaccattt gcaggctgca
      301 cttcaaattc tttgatcttg cctgcttgta gttgttctgt gaattttgag tactcgatat
      361 ctgacgatcg ctggccgtta ttaccaaaaa tgaaataaac gaccattacc atcgctaaga
      421 ttacgaggac ataatacaag gcatttttca tcatgccgtt gttctttttg ttcatagttg
      481 ccctccctaa gcgtcaattt aatttaacag ttttttatca atcctgacca ttctattgta
      541 ccataattta attgagatac agcattaatt tgattgataa acactaggtt tcaatacacc
      601 gatgtacgga aggttacgat atgcttctgc ataatccaag ccataaccga ccacaaattc
      661 atttggtacg ttgaagccaa cataatctgc ttcgatattt actactcttc cttcaggttt
      721 gtcaagtagt gtaacaattt tgactgattt tgctttgcgg tatttgaaaa gatccactaa
      781 ataagcaagt gttcttccgc tgtcaatgat atcttcaacg atcaacagat cgcgaccttc
      841 tacattcgta tctaagtctt tgacgatttt tacttcccct gacgagactg ttgcattccc
      901 gtagcttgaa acatccatga aatccaattc caaatacgta tcgatagaac gaacgatatc
      961 tgccatgaat ggtaccgctc cttttaagac acctactact aggggattgg tgttttggta
     1021 aatttctgtt aactctttgc ctagttcttt gcaacgaact tgtatctctt cttgtgagat
     1081 caaaattctc tcaatatcct tttccaacat caggttctcc ttccaattgc ttctttttga
     1141 tatttgtaaa gaagtatgta gtgtatttta tcagtttctt cacgaatact caaatatgaa
     1201 aaggcaaaag gtatcaagct atatatattt cttttggcat ctgtaatgat ccaagcatgc
     1261 tgcctttgtg aattaggtac tttctgatcg ataaaatatc tagatacttt ttttgtgaga
     1321 ttctctgtta atttaatgcg gtctcctgct tttctttttt cgacatacag ttgcacatca
     1381 gcaggaatcc aaatgtcatg agaaaataga gaccatgaat ctgttgcttt gatgtcccct
     1441 gcattttcag gtttgaccag accaatccat tctgtttcat tcaaatatat tccttgttct
     1501 ggaatcagca acaagcgctg gtcttctgtc tggctagttg cttgtttttc ttttttagag
     1561 agtacaacaa aatgatactc tcttttcagt atccaattat tgccaatact gagttgccat
     1621 tggctttttt tctgttgcca ctggtctaaa atcgactcca tttgttgttc tttcaaaata
     1681 attcctgttt ttgagaatgc tttgtcgaaa aaggagtaaa aaaaatagta tcgttcagca
     1741 tctgtcattt cttcaatcat ttctgcagaa aatcgaaatt gttcctccca ctgttcgact
     1801 tccttttcga tcaactgtcc catatatttt tgaatgactt ggtctgccca ttggatctgt
     1861 tgcgaaaatt gctggaaatg tctcaacgcc tgtgtatttt cttgtttcaa ttgaggaact
     1921 accaaatgac gaagccgatt tctctggata tctaacaatt ggtttgtctg atcctcaaag
     1981 taaacaagct gcgattccgc tgcataagta tataggtctt ctttgctaaa actcagcaac
     2041 ggacgaatca gtctgcccgt agcaaatggt tgtacttctt tgattccaga aaaagtattt
     2101 aactgtcccc cacgaacgat tttcattaac accgtctcga tttgatcatc tccgtgatga
     2161 gcagtcatca acgtgtcata ttgtccttct tccatcacgc gagtgaattc ttgataacga
     2221 aagttacgag cttctgtttc tatggcggat ttggctggat cttcccaaat acttacatat
     2281 aaagggagat tcttctcttc acaatatgag cgaagatagg cagcttcttc tgctgattct
     2341 tctcttaatt ggtgattgat gtggacgata cctaaagaaa aaccgatttg gcgttgcact
     2401 tgtaaaagta aatggagcaa aaccatcgaa tcaacaccac ctgataccgc tgctaaaatt
     2461 ctcgatcctt gttgccagaa tccttgttca ttcccgtgcc gaataaatga ctgctgtaat
     2521 agcggtgctt ttttcccgcc tttttcaacg ttcatccttt ccacaccctt tcttcctaaa
     2581 aaaaagagag cgggaaaacg tctaaaacga caattaacct gccctctgtt gatactatga
     2641 cccaaatagt cgcactatca ttatttgcgg ctttttagct gcgacgtccg ccacggccac
     2701 cgcgctttcc ttctgtgttt cgcttcaatg aagataagcg gtcatcgcta tctttcaaaa
     2761 atgaactcat caatgaatcg aagtcttctt tttgagctga aggttgactg cgagaaaaag
     2821 attttttctg tgctggacga tttcggttgt cacggggatc tttttgttga tattttttag
     2881 gacggctttc ttctccagat tcagcaggtt gttcctgcgc tttccggata gatagtccga
     2941 tttttccatc atcatttact gaaataactt taactgttac ttcatctcct acacttaaaa
     3001 catcatggat gtcttttaca tagccatttg acacttcgct aatatgaact agacccgttt
     3061 ttccagcacc tagatcaata aacgcaccaa aatttgtgat tcctgatact tttccttgca
     3121 gttttgctcc tacctcgatt gacataaaaa aaatgttcct cctaattatt tccctttttt
     3181 atattcctgc ttattttgca gttgtagaac tagttgattg atctgtttca tttgtcgagc
     3241 tgtctgtatt tgattctaat ataggataaa ctttttcacc gtcttttgag taaaagaatt
     3301 tgcttctggc aagtttagct acatactcat catctttcag caatgaaaca tcttggtgca
     3361 gctgatcaac gtctgcgttg acttctttca tttcagtatt tgcttcgact ctcaattcgt
     3421 tcaattgatg catcctttgc atgtcatgga aaatctgcac acctattatc gcgaaaatga
     3481 ccaaagcacc aacaaaaatt gcagccagtc tgcgtcttcg aaaaatcagt tgacgttgtt
     3541 gttgctgaaa ttcagcatac tttttctttg catattccgt atctagcgca gcgattttat
     3601 tgcttttttt cgtcacttgg attcgctcca atctttttga ttgtcttttt cattatacca
     3661 atatcgtgaa agcttgtcta atctatttct ctagtgtttc tgtgcgattt tcagaaataa
     3721 tttcgtacat tttttgtgca tcttcttttt tggttgattc atgtaattcc gtaattttca
     3781 cttccaacgt tttgttgcca aactggactt tcaaaatatc gcctactttg acatcactgg
     3841 aagatttcgc caatttaccg ttgatttgaa ttcttccttt atcagctact tcttttgcta
     3901 ctgatcgtct tttgatgatt cgggaaattt ttaaaaattt atctaatcgc atatcttatt
     3961 cctcactttt taattttttt atttaatcgc aggattttct taccaaaagg caacattagc
     4021 cattctcgta ctgtaaacag acgaagacga atcactgcat aaatgaacac agaagccccg
     4081 atcccaactc ctatcacgct gacaaaaaaa gcagtggaac gatgagttac aggaccaaat
     4141 actatattca atacaccata atacagcagc agtactactg tcatacaaag aaggctgaac
     4201 atcaacttcc gtccaaaatg ccgttctttc caaaaagcat ttagctcttt tgtttcaatt
     4261 cgaacaaaat accaaagcgt cacacctaat cctaaaaggg tagacaggct tgcacctgct
     4321 gttcctaata aaccggtcag aaaagaagta gtcaatcctt ttaccaatag tccccatcct
     4381 gcgcctttta aagaagggcg aaacgagttc ttgctttgag caatactttg ataagcttgg
     4441 atgactgcag tgaaagcaat ggaaaaaaca aataatacaa gcgctgcatt tcccgcataa
     4501 tctttaaaca acgcataatt gatataaggg agcagcaagg ctaatcccaa agacgcagct
     4561 aatgctaaag cggtcgtcaa tctaagatag attttggctg tctgtaaaaa ctgacgatag
     4621 atccggttgg tcaaatgacg agtcaacgca ggcaggaatg tcgcacttaa tgcagtggca
     4681 atcactaaac ctaattgtac taaaggctga cctcgatcat agaccccttt tgctatcttc
     4741 gcaccgtatt ctgacagtcc gtaaacttcc aaagcatttt ttacaaagaa agagtcgatc
     4801 agctggaaaa agataagcaa tccactataa atcgaaacca gtccgccttc taccagaaaa
     4861 cgccggatca agggctttga cggctgtttg aacaatggaa accgtcggaa gcttaacgct
     4921 cctccatgaa tcttttgttc atagtagtga aggatcccat atgcgcataa accgccaaaa
     4981 acagctccac tcattgctgc cgtccctgtt tggtacaccg tccatccaag ctttctaaaa
     5041 gcaaacgcac tacagacaat cacaaaaaca cgaacgaact gctcaactac ttgagaaaca
     5101 gcagttggtt ccatcaagaa tcttccttgg aaattccctc gataaaaaga tagaccaggc
     5161 atcagcaaaa aggtaaagga aactacttgg atcagcggtt tcaattgtat gtctcccatc
     5221 atccaagcaa tccagctgct aaatgcaaag acaaacaacc acaaaaagac acctgtccaa
     5281 aatactaagg gataaagatt ttgcaaagct tgctttcgct cgaaaggatt ctttctttct
     5341 gcaacgtact ttgatatgaa ctgcggaaga ccggataaag ctaaagtcat tgctatccca
     5401 taaattggat aaacttgctg ataaacataa aaaccttcgt cgccgactaa gttttggagg
     5461 ggaattcggt aaagggcact taatactttt gctatgaaag aagcaatcgt caatacaaat
     5521 gctccttgca tgacgcgccg catttcttta tgatccaaat cattgctcct tcctacgctg
     5581 acagatattt ttgctcacgc aacgctttga caaacttttg gatttcctga agccatactg
     5641 cttcttccat ttggttgggg atcgtcaagc gtatcaccat ctgttctttt tcaacgccta
     5701 gactggcttt tagttttgtc gctgtcagtg cttcgaataa ctgttccaca ctatagcgtt
     5761 ttgttccaac tttacttaga gtaaagacta tatgcgcctc ctgtttcctt atcgtttcta
     5821 ggagcgctcg atcgccatcc attttgatct gcccaattgt caagagatgt gctacttctt
     5881 ctggatattc tccaaaacgg tcgagcagat catcttctag ttcatctagc atttcttgcg
     5941 aatcaagttc acgaattcgt ttatagatct cgattttctg ccgttgatct gccacgtatg
     6001 tttcaggtag atacgcatcg acaccgaggt cgatctcaac agtggtcttc tccacttgac
     6061 tattctttcc ttgtttacga gaaacagctt cgcttagcat ttgagaatac atatcgaaac
     6121 cgactgcatc aataaatcca tgctgctgag cacctaagag attacctgca ccgcgaatgg
     6181 aaagatcccg catcgcaatc ttgaatccag atcccagttc tgtaaaatct ttgattgcct
     6241 gaagacgttt ttcactgact tcgttaagga tcttctgctg ctcatacata aagtaagcat
     6301 atgcgacacg gctgcttctt cctacccgcc ctcgcaattg gtataacgtg gataatccca
     6361 tataatcagc gttctcaacg aacaacgtat ttgcgttcgg gatatctact cctgtttcaa
     6421 tgatggttgt agtgacaaga acatcatatt gaccttcaat aaattcaaaa agcgtatttt
     6481 ctaattgcgc ctctgtcatt tgcccatgag cataaccgat ccgtgcttcc ggaacgagtt
     6541 cttggatttc ttctactttt tgttcgatcg tttctacccg attatataaa tagaatactt
     6601 gtccacctcg tcccatttct cgatggattg cttcgcgaat agccccagga tttttttcca
     6661 taacatacgt ttggatcgga taacgattcg caggcggtgt ttcgattact gaaagatccc
     6721 gcacgcctaa catcgacata tgcaaagttc tcggaatcgg tgttgcagtc agagtcaaaa
     6781 catccacttg tgcacgtaac tgtttcaatc gttccttatg ttttacacca aaacgttgtt
     6841 cttcgtcaat gatcagtaga cccaaatcgc taaattcaat atcttttgac aaaattcggt
     6901 gggttcctac aacaatatca acttgacctg tacgcagttg ttcgatggtt tctttttgtt
     6961 gctttcttgt tctgaaacga cttaatagcc cgatatttac ggggaaacct tcaaatcttt
     7021 ccagcatcgt ttcatagtgc tgctgtgcca agatcgttgt tggaactaaa aaggctactt
     7081 gtttgctttc ctttatcgct ttaaaggccg ctcgaagcgc tacttctgtt ttcccataac
     7141 caacatcacc tacgagtagt cgatccattg gtttttcttt ttccatatcc cgtttgatct
     7201 ccgctgcact tctcaactgg tcatcagttt cagagtaggg gaaggcattt tcgaattctt
     7261 tttggtaggc gtcatcaggt ccaaaagcat atcctttttc tgactctctt gcggcatata
     7321 atttgattag atcgtcagcg atatcctcga tttttgaaga gacttttcgc ttggttttcg
     7381 tccattcact tccgcctagc ttattgatgc gaggcgcttt ggcttcagag gcgacatatt
     7441 tttggatcag attcagttga gtaacaggaa taaacagttt gtcgtcgttt tgatacaaaa
     7501 tagtcatata gtcttggtga acgccatcta cttctagcgt ttccatccca atgtatttcc
     7561 cgataccatg attggcatgg acaacgtaat ctcctgcttt taactcatta tagcttttca
     7621 atcgctctgc gtttgttact gtttgacgtc tcgtttgttt tttcgtcgtt ttttggaaaa
     7681 tttctttttc tgtgatagct actatttttt cttgaggaag ttcaaaacca gcctgcaaag
     7741 ctccttcaac taattgtacc tgtcctctga tcaatgcatt tttttctgtt tctactacta
     7801 atatgtcttc atctcgaagc atttgttctg ctttgcgtat tctgtccttt gtcggaataa
     7861 agatcacaac agtctgtttt tgcttgcgcc aacggtccat ttctgttttc agcaatggca
     7921 tttgtccgaa gaactgttgc atcgtccgat attgaaaatt atggattgct tggaaacgaa
     7981 gatttcccat ccctttttgg aagagtgaga aaaaggttgt ggtgaacttt tccttctgca
     8041 cgattttttg tatatctgct gaaaatgtct gctcagaaaa cacccgcaat tcgctgattt
     8101 tttgagtctg ccattctgcc gcttcccttt cgatttcccg atttgtttcc atgattcgct
     8161 gataatcatc aacgactagt aaactatttg aagggaaata atccaagatc gtcacagatt
     8221 cttggtaaag aagatctgag tagaattttg cttcttctgt cgggacacct gcttgccagc
     8281 ttgaagtcag ctgaccgaag taatcttgca aaaactcgcg gtctgcttct tctgtggtcg
     8341 cagcaagccg tttttctagc attttttgca tttgctctat tccatgttct agatcctctg
     8401 gtccgtagac ttgttcactc gttggggaaa cccagatttc ttccgttttt tcgattgagc
     8461 gttgtgtttc tgcttcaaaa taccgcatag aatctatctc tacatcaaat agttctacac
     8521 gaacaggata ggttgtcgtt aacgggtaaa tatcgataat gcttcctcga atactgaatt
     8581 ctcccggttt tgcgaccatc tcttctcgta catagcccat caaagtcaat tggcgaggaa
     8641 gattttccac gtctatctca tcaccaatat gccaattcaa ttgcgcctgt agccaagttt
     8701 tcttattcgg cagtcgtttg tgcaaagatg cgactggcaa caaatagata cctgcttttt
     8761 tttcttgaag tgcgttaagc gttgcaactc tctcgcctcg cgcttctgga gaagaaaagg
     8821 ccatttcagc agaaagcact tcatccactg gaaataaatg gatctgatct tgcggaatca
     8881 catgttgcag atcttctgct aactgattcg cataatacaa atttggcatc agaataatca
     8941 tgtttttatt tgttttctta aacgctcgag caaatagaag tgttttcgct gagcccgcta
     9001 atcctgtgat tagttgtcta ggcatttttt cttctaaatt gctgacccat tcttttactt
     9061 gcggatcttt tccgactaat tgaataatat ccattccttt tcctccttcc tcaattgaat
     9121 cgattcatcg catccacgaa cgaatgccct tctatgagat attcgactgc tttaacagat
     9181 tgacacatgc tttcttctat caaaggctga ttttctttgc tgaaaggact aagcacatgc
     9241 tggactactg tttttttccc ttctggcctg ccgattccca ccttgattcg atcaaagaca
     9301 ttcgtattca aatgggaaat aatacttttg ataccgttat gcccgccggc acttcccttt
     9361 tgacggagcc ggatttttcc cactgccaga tccaaatcat cgtagattac gactaattcc
     9421 tctggataaa tcccaaaata agtcattagt gggccgactg cacgaccaga ttcattcatg
     9481 aatgtttgtg gtttgactaa aaggatcttt tctccattat gaaaaaattc ggcaacttct
     9541 gcttcgaacg gatttttttt gaaagtagct tgatgctcgc gtgcaatttt atccacgacc
     9601 ataaagccga tattgtgttt tgtatattgg tatttggtac ctgggtttcc taacccgacg
     9661 atcattttca tttttgtttt ccacctaatt ctaaaaagca gcacaaaagg ctggactgct
     9721 aattagcggt ccagtccatc gagctttttt cttttttata cttgtcaatg catagtataa
     9781 cacaaaacaa ggcgtttcgg ctaaaagaga atcgttaata aatgataaag aaattaaaaa
     9841 tttcttcaaa gtttaatgaa ataattgata tataaaaata gtgaaataac gcacatgtaa
     9901 gcttttacat ataatacaac cttgttatct gttatatttt atagccaaat agcgtgacat
     9961 ggggcttgag aaatgttagc ataagcatgg gaaaaaacaa atcaatggaa ggattgatac
    10021 taaatgactg cagctgcaga aaaaaaagat catcaaaaag ttattcttgt tggagacggc
    10081 gccgtaggtt ctagctatgc ctttgctcta gttactcaaa atatcgctca agaagtaggt
    10141 attgtggata tcaatactgc taaaactgaa ggagacgcaa ttgacttgtc tcacgctttg
    10201 gcatttactt cacctaaaaa aatctattct gctacttacg cagacgcaca tgatgctgac
    10261 ttagttgtta ttacagctgg cgcaccacaa aaaccaggtg aaactcgttt agacttagta
    10321 aacaaaaacc taaaaatcaa ccgcgatgtt gtcacacaaa tcgttgaatc aggatttaac
    10381 ggtatctttt tagttgccgc taacccagta gatattttga cttactcaac ttggaaattc
    10441 tcaggtttcc ctaaagaacg cgtaatcggt tcaggtacct ctcttgactc agctcgtttc
    10501 cgtcaagcac ttgcggaatt agttgacgtt gatgcacgta acgtccatgc ttacatctta
    10561 ggtgaacacg gagattcaga attcccagtt tggtcacatg caaatgttgc cggcttgcaa
    10621 atctatgaat gggtaaaaaa caacccagaa atcgatgaag aagctatggt taacctattc
    10681 tttggcgtac gtgacgctgc ttacacaatt atcgagaaaa aaggcgctac tttctatggt
    10741 atcgcagtag cattagcacg tatcactcgt gccatcttag atgatgaaaa tgctgttctt
    10801 cctttatcag tattcatgaa tggcgaatat ggattgaacg acatctacat tggtgcacct
    10861 gctgttatca accgtcaagg tatccaaaaa gtaatcgaaa ttcctttaag tgacagcgag
    10921 caagaccgta tggctgcttc tgctaagcaa ttaaaagaaa ttctagatga agcttttgct
    10981 aaactagacg ctgaataatc tttgaacatg acggaaaaca tctattcggt gttttccgtt
    11041 tttttatttt atcaccctta tggtttctaa attttttcat aaagtagaga taaccttcgt
    11101 cttttcctct aaattatgct atcatgagac cgttccaccc taaactttta cttaaggagt
    11161 tgtcgatatg acaagatctt cgcatcgaaa aaaatcgaaa catgttgctt tgctttcagt
    11221 tcttggtgtt ctcttagtgc tgatcagctt ttatacctat cgcagtacat actacacaaa
    11281 gcattttttg ccgaatacag aaataaatgg aataaatgtc agcaatctaa cagtcgaaaa
    11341 agcaaatgaa aaattaaaag aagcttattc tgataaaaaa ctggctatta aagaagatgg
    11401 aaaagtctgg caggaagtac caaaatccga gctcggctac aaagatgatt tcacttcgga
    11461 gctttctccg atcctaaatg aacaaaacgg atggacttgg ggaatgactt atgtctcagc
    11521 ggcagaaaaa caagaaatag atcctgtcag tcaaaaccac caaaaactag acactaccgt
    11581 tcagacattg acaactaagc tgactgattt gaacaaagac cggacaccta ctactgatgc
    11641 aacgatcgaa aaagcaggtg actctttcgt tatcaagcct gaagtcaatg gaaatacgat
    11701 cgacgtcgat gctgcggtca aacaattaaa aagtgcggta aactctggta aagatacaat
    11761 tgaattgact gaattcaaag aaaaaccaaa agtaacatct gaagacagct cactgaaaga
    11821 acaactcgca tccatgaatg ctatcgcaaa tgtaaaagct acttacagta taaacggtga
    11881 gactttccag atcccatcaa gtgatattat gagttggctg acctacaatg atggaaaagt
    11941 cgatttagat acagaacaag tacgtcaata tgtaacagat ttaggcacaa agtataacac
    12001 aagtacgaat gacacaaaat ttaaaagtac aaaaagagga gaagttacgg tcccagttgg
    12061 cacttacagt tggacgatcc aaacagacag tgagacagaa gcattgaaaa aagcgattct
    12121 ggcaggccaa gattttactc gttcacccat tgtgcaaggt ggcacaaccg ctgatcatcc
    12181 attgatcgaa gatacgtata tcgaagttga tctggagaac cagcatatgt ggtattacaa
    12241 agacggaaaa gtggcccttg aaacagatat cgtttccgga aagccaacta cacctacacc
    12301 tgctggagtc ttctatgttt ggaataaaga agaagatgca actttaaaag gaacaaatga
    12361 cgatggaaca ccttacgaga gtccggtaaa ctactggatg ccgattgact ggacaggtgt
    12421 tggtatccac gattcagact ggcaacctga atacggtgga gacttatgga aaacccgcgg
    12481 ttcacacggc tgcatcaaca ccccaccaag tgtaatgaag gaattattcg ggatggtcga
    12541 aaaaggaacg cctgtattgg tcttctaaca aaaataatat ataaaaagaa agcacccgta
    12601 cgattggtac aggtgctttc tttttattat ctaacaatgt gctacttaca tttcagcagc
    12661 agtaaagact tctgaaacat catcatcgtc ttctagctta tctactagac gttccaattg
    12721 ctctttttgt tcctcattta aatcaatcgt cgtttgtgga atcatcgtca attctgcttg
    12781 cgccaaagtg aactcttttt ccaactcatc tcgtacagca gcaaaatctt ctggtgcagt
    12841 ataaatctcg aatacttcag gagaagtttg cagatcttct gcccccgcct ctaatacatc
    12901 ctcaaacatc gtctcttcat caacagtcaa cccatcgcgt tcaatagcga tatatccttt
    12961 gcggtcaaac aagtaactga ctgaacccgt ttctcccagt gatccaccgt ttcgagtaaa
    13021 agctactcgg acatttgtcg ctgtccgatt acggttatct gtcaatgcat ggactaagat
    13081 tcctactcca cccggaccat atccttcata cgtgatttca tcgtaattcg tttcatctac
    13141 ggcactcgta gcctttttaa tcgcccgttc cacattgtcg ttaggcatat ttgcagcttt
    13201 tgctttgtcc acaacaagcc gcaaagctgg attcatagat ggatcaggac cgcctgcttt
    13261 agctgccata taaatttcac gagaaagttt ctgaaagact ttccctcgtt ttgcatcttg
    13321 ggcatttttt cttccttgga tattatgcca tttactatgt cctgacatga tttttcctcc
    13381 cttgttcttt tgagaataca cttctattct agtactattt cacaaaaaaa caaagccaca
    13441 gccaatctat aaacgcttct tgaatgccgc aaaaagcaca cataatatca cagcagtcag
    13501 agcaccgatc gaatccaaca tgacatcttg gaacataggt gttcgtccac cggtcatcat
    13561 ctgatggaat tcgtccaaac ctgcgtaccc tgttgctgaa agccaagcaa ggatcgctgt
    13621 cagccaccaa atttttagct tggggaaaac acctaaaaac aagctgcctc ccaaaataaa
    13681 ataggtaccg aaatgtgcgc ccttccttaa aaagaactct aaaaatttgg cgtagcccag
    13741 atggtcgata ctcaccatac ttccaccata attaaaacgt atacccgcta atgtgtcttt
    13801 aaatggctga ttaggcagcc agctttctaa tcgcgaaact tgtgattgct gtccgtaagt
    13861 ttgagaagaa ctgtaaaata agattataac gactatcagt gcgattacta aataaatatt
    13921 tccgtttttc atttgttttt ttgaagaatt cataagtcac ctctgtattt tatcttagac
    13981 tttttttgaa gaaacaacaa tcattttcat agagacttct tccataaaaa agtgctatac
    14041 ttaattttgt attacaatat gaagggagtt ttttcatgag ttattcatta aattggaatc
    14101 tagattctat tttttccgga ggcagtcatt ctgatgcttt gaaccaacgg atgaagcaat
    14161 tggaagacca aatgaacgag tattatcacc gcgtaacaaa atggtcacct agttctgaca
    14221 atgcagagca gttaaacgct attcttcaat tgcaagaaac gattacgaat ggttttaccc
    14281 aatgcagcag ctatattact gcattgcttt ctgccaatgt caatgattca gatgcaaagg
    14341 tcttatcagg caagctttac gcaatgctgc ctcgtctgca atcagccgaa actgtattgt
    14401 caaagaaatt tgcagaaata tctgacaatg attggaatca gatgctttct caatcttcgt
    14461 ttgaaacaat tgcttttcgt ttaaacgaga ttcgtcgtga tggctcacaa ttattatcag
    14521 aagctgaaga aaacattata aatacgcttt ctttagatgg tctaaatgct tggagcagtc
    14581 attatgatac gattgtagcc agcatttcga ttcccttcga acaagatgga caagtagtcg
    14641 aattatctgc tggacaagcc ttcaataaga tgatgggcga tccagatcca aaagtcagag
    14701 aaacactttt cgcagcatgg gaagaagcct ggaaagaaaa ggcacccctc ttcacggata
    14761 ctttgaatca tttagatggt tttagacttt ctgactatga acttcatggt gtcacagatt
    14821 ttctacaaaa accattagag tacaatcggt tgaagaaaga aacattgtct gtcatgtggg
    14881 atacaatcca gaaaaacaaa caaccccttg ttaattactt gactagaaaa gctaatcttt
    14941 tcggtaaaga aaaaatggaa tggcaagatc aagatgcacc tattatttta ggcgatttga
    15001 aagaaaaaac ctttagtttt gacgaagcag cagcatttat tatcgaaaac tttaacaaat
    15061 tcagtccaaa aatggctaaa tttgcccaat ctgcttttga aaagagctgg atcgaagcag
    15121 aagatcgtcc tgggaaacgt cctggtggtt attgtactga gttaccagaa acaaaagaat
    15181 caaggatctt tatgacctac agtaattctg ttaatgaggt tgcgacactt gctcatgaac
    15241 taggacatgc tttccatagc agcacgatgt gggatttacc ttctttaaat cgcgaatatg
    15301 ccatgaatgt cgcagaaaca gcaagtacat tcgcagaact gatcgtagcc gatgctacat
    15361 taaaagtcgc aaaaacaaaa gaagaaaaaa tcaatttgct agatacaaaa ctgcaaaatg
    15421 ctttagctat gttcatgaat atccattccc gattcatttt tgaaaatcga ttctatgaag
    15481 cacggcaaga aggattagtc tcagaagata agatcacaga gatgatggtt gaggcgcaaa
    15541 aagaaggcta tcaaggagct ttagctactt accatccata tttctgggct gcaaaattgc
    15601 atttctttat tgatgacgta ccgttttata acttcccata cacatttggc tacctcttta
    15661 gtttgggcat ctatgcttac gccaaccaaa agggagcaaa ttttgaagaa gaatatatcc
    15721 atttactacg cgatactgcc tcaatgacga cggaagatct tgcgaagaaa catttgaatg
    15781 tcgatcttac ccaaccagat ttttggcaag caggtatcga catggtgact gaagatatcc
    15841 aaacgttcat ggaattgaca gaagaattta tctgatttct attttatcac aataaaaaaa
    15901 gagttagtcc agaatgggct aactcttttt gattaatcat tcttacatgc tgacagcaaa
    15961 gtcaggggca aaccattgaa ctgaaccaac ttttacgctt tcgcctggtt gaggagcatg
    16021 gatatattgt cctccgccta aagcaatcgc tacgtggtaa gttccgcctt gtgaccccca
    16081 gaataacaag tctccagctt ttgcttgaga aactgaaatt tttgttccag ctgattcttg
    16141 aggaactgtc caaccaccga tgtcacgacc agttacttgc atgtaaacat agcgtgtgaa
    16201 ccctgagcag tcaaagccac ttggatcttt accgccccaa acatatggag taccaatata
    16261 tttgtaagct tctgctacga ttgctgcacc attcacactt ccagaaggag ctggagcagg
    16321 tgttacaggt tttggttgtt caggtgttgg tgttggcgtt ggagccggag tactacttcc
    16381 tgtgccgttt cctgtatcaa cactttgatc tgttgatggt gttgtaggcg caggtgttgt
    16441 tgattcttct gttgttgaag attcaggcac tgttgttgat tcttctgttg ctgaagattc
    16501 aggcactgtt gttgattctt ctgttgctga agattcaggc gctgttgttg attcttctgt
    16561 tgctgaagat tcaggcgctg ttgattcttc tgttgaagat tcaggtgttg ttgattcttc
    16621 tgttgctgaa gattgtgtag ctgatgatga ttcttcagtc attgaagatt gtgtagctga
    16681 tgattcttct gttgctgagc tttgtgcagt agatgaagct tgagtagctt gtgcttcagc
    16741 ctctgcttgt tcgcgtgctt ctttttcagc tttttcttgt gcagcttgtt gacgagcttg
    16801 ttctgctaga cgttgttgtt cacgaatacg agcttgctct gcttcagctt ctgctttttg
    16861 acggttcaag tcagctttct tgtcttcagc agttgcttgt tcagcagcta atgaagtttt
    16921 cagtacgttc aaatcagctt gtttagataa aagatcgcct ttttgagatt ccaaagcagc
    16981 ttggttttca gctaattctt ttagtttagc atcgttttca gcttttttgt cttctacagc
    17041 ttttttatct tgtttttgtt gttccatcaa gtcgttgttt gcttttacca tcgttgtcat
    17101 tgcttgaacg cgtccaatag catctgctaa tgaatcagca ttcaatacag catcgatgta
    17161 gtttgagctt gtgttgctta cttgtgcttc gcgtgcttgt ttttggattg tatcttcacg
    17221 tttttcaata cgttcttgta aatctgcaat gtcttttact aattgtgcag attcttgacg
    17281 caatgtatct tgttttgcta ataggtcttg tgcttgtgtg ttgatttcag aaacttggct
    17341 ttctaaggca tcgatttgtg attgtgcatc tgcttgttga tttttcaagt ctgcaatctt
    17401 ttgatcttgc tgttgtatct gagaatcaaa atcgtccgcc gcggcagcga tcggtgatgc
    17461 tacggcagtc aaggtcattg aacataccat tactgctgat attaaactct ttttcactct
    17521 tcattcctcc gactggctta atttaataaa tatctaaata tatttaagtc tttcttaacg
    17581 atataaaaat tgtaccatag cagtgtgtca acgatataat agttatgtta caaaaatgtt
    17641 tcaaaacaac ttttaaaaat gacaaaactg ttcgtttcag aaaatgatca tgaaaaagcc
    17701 gcgttaagtc tagcatttct taagataaca acgattcttt ttcgctttcg acctaaaaat
    17761 aaaaaaagaa attattcatc actgaataat ttcttaaagg gaaaaatgaa taatacaaat
    17821 ataatcatgt tcaacagcag tgtaggtcct aaaaaccttg taataaaata cgtgttattt
    17881 actactgcta atttaaaaat caactggacc accatcgtaa acaattcata acccgtgaca
    17941 aagatgatca tactgaaaaa ttcagtaaag atgttgacat ggataacgtc tttcattaca
    18001 tacatcaacc atacaattaa agggagagct actgcataga taccgataac gccaatatag
    18061 taagcgtcat agatagcccc gagaacaatc gtggtaatca gtaaataacg tttttcaaac
    18121 gccatactgc aacataataa tgccaatatc agaaagtggg catttgacat ataggtgcct
    18181 ttcgaccact cccctagcat tcttgttaaa tgaccatcta tcaacatcaa aaagaacaaa
    18241 acgattggca gatagtactt cattgtttct ttcttcaaca tggactaatc ctccgctagc
    18301 cgttggacaa tcgtcacaac tgacacgtca tacatttctg cataaggttt cacataaact
    18361 tcccgatcca gcccataact atctggtttc gttttaataa ccgtaccgat cggcaagtta
    18421 gctggagaat tccctccaag accggatgtc tggacaacat ccccttcttt gatatccgta
    18481 tcaccagtta attgggtcac cactaaggca tggagctttt catcatagtt tttaagcaaa
    18541 ccaaacgctt cgccattagc cgaagatacc cgtactggaa aatgattgga gctttcatta
    18601 gaggatgaca gtaattcgat tttagacgaa gccgtattga cctcgatcac tcggccaatc
    18661 aatccttttt gcgacatgac tgccatattt gcttcgatac cgtctttaga acccttgtct
    18721 acgatcagca tgtcctgcca actgtcaggc gaacgagtaa tgacatttgc cgttactttt
    18781 tcgtaactag tcagcgtctg attcaactca agttcttttt tcaacgcttc gatttctctc
    18841 tcgtaattct tgttttgctg tgaaacgtca tcatagctat cgattttttc ttttagccgc
    18901 tcattttcgg cataagttga aaaaagatta tggacagaat ctactccatt tcgtacccac
    18961 cgtactgggg cagagattgt tttatcaacc attgcaacac tgtcattaac gatggattgg
    19021 aagaaattgg gctttccttc attcgctcgc tgcgcagcgg tcacacttag tatcgtcacc
    19081 acgatgataa caataaccaa ggtaataata atatttttat taggattgaa cttcttcaca
    19141 aagacacctc gaattaatta gactcttgtg ttcaatttta tcactaaatg ccgtgaaaca
    19201 aaaggtaagt tgctcctctt aagctttttt ttagcaaata atagattctg tcatttttag
    19261 cctgaaagac aaattttttt atttgtttat ggatcaatct ttgctttgaa taactgcaat
    19321 cataccatct gaaaaagaaa attctgcggt atctgaaaga aattcgttac ttgcataaga
    19381 tgtcggatat tctacttctt tattttccaa tacatacttg ctgcctttta atgtaagatt
    19441 cgagacggga gtcaagcagc aataagctaa atacttcata tctggtatct gtcttactat
    19501 atgctctcca ggcaaataat aagacaaata attttgacaa tctctgataa taagctgatg
    19561 ggcaaagggt ttgaagcgag gttccatacc taaccagaga ttggctaaaa gatgatccaa
    19621 tcttccgccc gtagctccaa tcaaagtgac ttccgcttgc gggtaacgtt caaatgtttt
    19681 taagatacct aactgtgtat ctgtatcgtc tttttctgca ggggaacggg tatactcttt
    19741 ggcttttgca aatacttcct tctgctcttc gttcgacaat gaatcaaaat cgcccactgc
    19801 cagatctact gggtagcctg cttgaagcag gtacagcgca ccacggtcta tcccgacata
    19861 ggcatcgtat ttggacagca gagactgcgg ccagttttgc ggattacctc cagcagctaa
    19921 taaaattttc atttagtcta atgcctcttt taatcgttcg atttgtccga ctggattttc
    19981 ggctccatag atatatgatc ctgcaacgaa gacatccgcc cctgcatctt tacagatttt
    20041 tgctgtttct ggaacgattc cgccatctac ttcgattgca taagtccaat tgttctctct
    20101 acgtagatca gcgagttccg taattttttc tactgtttct tcaataaagc tttgtccgcc
    20161 gaacccagga ttgacagtca tgactagtac ttgatcagcc aaaggcaaaa catgtttgat
    20221 catggagact ggtgttcctg gattgataac tactccagct ttaactccta agctttttgt
    20281 catttgaagt gcccgatgaa tatgaggagt tgcttcgacg tggacagtaa tgatatctgc
    20341 tccagctttt gcaaatgctg gaatgtagtt ttctggatca acgatcatca gatggacgtc
    20401 taacggtaat ttagtaacag gacggattgc ctgaacaata tttggcccta atgtgatatt
    20461 tgggacaaat tggccatcca ttacatctac gtggatgtaa tctgcgcctc ctttttccac
    20521 taactcaatg tctctttgta aattggcaaa atctgcactc aaaatggatg gtgctatctt
    20581 cattcttgtt tcctcctatt ttcgtttacc ttcttttttt gcatacattg gttttctttt
    20641 ttcgatttct tgtaagaatt gcaaataatt gtcgtaacgt gtctgtgcga taccacctgt
    20701 ttctactcta tgttttactt cgcagccagg ttctttagca tgcatgcact ctctaaattt
    20761 gcaatggaca gaagcttcta caaattcagg aaattgtctc ggcagttctc ctgcttcaat
    20821 tgtcaaaaag tcaatcgagc taaatcctgg tgtatctgct actaacccgc catagatagg
    20881 aatcagttcc acatgacgtg ttgtatgccg tcctcttcca agtgcatcag aaatctctcc
    20941 tgttgctaaa ttcagttccg gtgatatttg attcagtagc gtcgattttc ctgctccaga
    21001 ctgacccata aagaccgata gccgttctgg gaagtaacgg atcagttctg tcgtatcacc
    21061 cgtatgattg ggtacaatca ccggatagcc tattttagta taggtttgct tgatctcttc
    21121 gattttttct gggtcgtctg acaagtcggt ctttgtcaaa aaaataatcg gttcgatcga
    21181 ctcgtattcc aaggtaacta gaaagcggtc aagtagatta taagaaaatg caggctctac
    21241 gatacttgtg acaatgattc caagatcaac atttgtgact ggtgggcgaa ctaattgatt
    21301 ttttctcggc aagatttcca gaagatagcc gtctgtttga ttttcactct caaagatcac
    21361 ctgatctccg acaagcggcg tgattttccg attccggaaa tttcctcttc ctcttgtttg
    21421 ataggtctct ccgtttgcgt atacatagta aaaaccgctg attgcttttc tgatttgtcc
    21481 tttgataagt gccacctccg tttttgttta ttttaccata cgaagaggaa agtaggtgtt
    21541 attccatgca aacttctcaa agtatatggc ttgaaaattg atcaaaagag atagaaacac
    21601 aaaaaagaga atgcaacaaa tcaatgtcgc attcccttct gatatatttt attcagcact
    21661 tattttgaac tttctgaggt gctagctgat gaacttgtgg agctggaaga ttcttctttt
    21721 gaagagttgc ttgtgctact ggagctactt gagctgctag aactgtctga agagtctttt
    21781 tcagaactag aagacgctgg atctggtcct ttagagtaaa ttacgttcac aaccattcct
    21841 ttggtaattg tcgttcctgc ccctggtact gtacgaatca ctttatcttt atccaccgtg
    21901 tttgaatatt cggaagtctc atgcccgata tattctgcac cgacactagc taaataactt
    21961 tgtgcttcgc tttttgtata gccggagata tcattcaatt taggctctgt tccttggctt
    22021 acgacaaaat tgatgtaagt ttcttctgct acaacttcac ccgaggctgg atcctgactg
    22081 atgatcgaac ctgatggtac agtgtcgcta tagtcataag tagcactaat attattgata
    22141 ttgaatcctt gtgtaacaag ccaattacga acagtttcgt agttttctcc tacatgacta
    22201 ctcaaagtca ctgtttctgg tcctttgctg actgtgaaag taatcgtgtc tgttttagga
    22261 tcgacttctg tatcttctga tggagattgc gaaatgatca ttccgctttc tacctctgaa
    22321 ctgaattcct cttctttttg aatcaagtca gcagaaaacc ctaattcttt caaacttgct
    22381 tcggcatctt cataactttt accactgtaa tcatctaatt tgattttttt cgtgccgcta
    22441 ctaatataca gagtgacttc gcgtttttgc ttgactgtcg ttcccgctgc cgggtctgtc
    22501 ttcactacat taccttcttc gattttgtca tctgcaattt cttctgtttt tgttgctact
    22561 tctaatttcg cttttgcgag tgtttctcta gcagaagctt cagaaagatc agaaacatca
    22621 ggaacaacaa cttctcctcg cccgccagaa gcaaagaata cagctgttcc gattcctaaa
    22681 acagcaagaa tagctaatag gatcagccac cattttttct gtcctttctt tttaggatca
    22741 ggcagaactt cttcctcctc tggcaaggtt tcgttttctg gcttgtcttt tggcagaggc
    22801 atagatttga atgaatcagg cattggtgca tcttccgtaa ggggtgtgat aacttttgtt
    22861 tcattatcca tcacatgagg ctgccatttt tcttcgtttg cacgagctgg tgataatact
    22921 gtagaaagat catcagacat ttcctcggct gacttataac ggtctgcagg ttctttagcc
    22981 gttgcccgta aaatcacgtt ttccagtgct tgtggcacat ttggatcaag tgcttttata
    23041 gatggaagat catcttgaaa atgttttaaa gcaattgtta cagcagattc accatcaaac
    23101 ggtacgctac ccgtcaacat ctcataaagg atgatcccta aagcataaat atctgactgc
    23161 ttcgttgcca tacttcctct ggcctgctca ggggacagat aatgaacaga tcccagcatc
    23221 gtattcgtct gcgtgatcga tgtttcagat aaagcgatag caatcccgaa atccgtgatt
    23281 tttacaacac cttcattatc gatcagtacg ttttggggtt tcaaatctcg gtggatgatt
    23341 tgatggttat gagctaaaga aatagcagaa aggatctgct gcataatatt tactgctgtt
    23401 tcataaggaa caggataatg tgtttggata tatcgtttca agtccattcc tttgacgtat
    23461 tccatcacca gatactgcat gttgtcttct tcaccaacat cgtaaacact aacaatgttt
    23521 ggatgaacga gttctgttgc tgctaaagct tctctctgaa atcgtcgaat cgcagtctgg
    23581 tcattttgaa agtcaaatcg taagacttta actgctacat cacgatccaa gattaaatcg
    23641 tgcgccaaaa atacatttgc cataccaccg ctaccgatat ttccagtgat ttgataacgg
    23701 ccatttaatt ttttgcccaa ctcgatcata cactgtcctc cttatattcg ataagaagaa
    23761 cggtgatatt atccgctccg cctgcttcat tggcacgatc aatcagctca gttactttat
    23821 gagacaatgg gccttcttga ttgatgatcg ttcctatttc ttcttcagac agcatgttcg
    23881 tcaagccatc tgaacataac agcagtcgat cttttagttg ccaaagatga gtagaaacat
    23941 ctacttcgac tgttccaggc atgccgacag aacgtgttaa aatatttttc cttggatggt
    24001 tcactgccat ttcttcgctg atttctcctg atttgactag ttcattgact aaagaatgat
    24061 cctccgtcaa ttgcatgatt ttatcttcac gaatcaaata agctctgcta tccccaacat
    24121 tggcaatcgt gaaccagtca tctgacagag cggccgctac gattgttgtt cccataccgt
    24181 tatattccgg ctgttcttgt ccacgctgat aaatcagcat attttcttct tgaatcgatt
    24241 gaatgaacca ttgggcgatt ttttcatcgt caacagcttc ttgtttttcc caagcttcgc
    24301 ctagattcgt taccgccatc tgactggcaa tgtctccggc acgatgacca cccatcccgt
    24361 ctgctaatac agcaagcttg agacctgaac ggctggtaaa tacactggca tagtcttgat
    24421 ttgtgttacg tcgtcttcct acatctgatt gatattcaat ctgcattttt tcacctcatt
    24481 actttttgcg caaacaacaa atgaaaaatc catctgtgta aaactgatgc ggatacaaag
    24541 tcaacatttt ctcttcgatt gatgattgta gcataggatt ggtcaaaaca tcaatcctct
    24601 caaaatcagg gtgtctttcc aagaaagcag aaacaacgtc ttggttttct tcttttaaga
    24661 tcgtacaagt gctataagtc agaataccgg aagactttaa agttggtgcg cagctttcta
    24721 aaatttccaa ttggattttt ggtaaattcg ctaattcatc cggctgtttt ttataacgaa
    24781 tatctggttt tcgccgaagc agcccaagac ctgaacaagg cgcgtctact aaaatccgat
    24841 caaactgctc tgcttcgaac tgtttactga cttctctagc atccatcttt tgagcgtaga
    24901 cagcagaaga gacacctaga cgtgctgcat tatcttcaat gagtttgact ttgtgttcgt
    24961 gtacatctaa agcgaccact tctccaccgc atgacggatc taaaaaagaa gcaatatgcg
    25021 tcgtcttacc tccgggtgct gcacaggcat ctaagacatg atgttcaggc tcgatttgca
    25081 tagacggcgc tacaagcatc gaactttcat cttggatcgt catgacgcca tcgtggaaca
    25141 atgagctaga agctaaatgc ccttttttcg ctactatacc gtatggactg acttggcttt
    25201 tttcagctgt gatctcttct tcttgcaaaa cagtgatggc ctgatctctg gaaagtcggc
    25261 gtgtatctac tcgggcacta gcatggctag gctgatatag cgataaaccg agcttttcag
    25321 tttcttcgta tccgatttga tccaccagtc gtttcgtcat ccatagaggc aggctgattt
    25381 tgacagaaag tctttcgata ggatcagcaa tgccttgcag atctggtgct ccttgacgct
    25441 gataattcct caagacacca tttacaaatt tccctgtccc aggatttcct tttacttttg
    25501 cgatttcgac cgcctcgttt aaaatagcat gtgcagggac tttatctaaa aattctaatt
    25561 ggtatacaga aagataaaga agcgtcttga cccagtcatc cacttttttt gctttagcga
    25621 tcaatggtgc aacgtaaaat tccaatagtc gctgatggct aatcgtacca tagatcaatt
    25681 ctgtaaataa acccacatct ttttgattta gctcgctttg tttgatcatc tcattcaaca
    25741 aaagattcga gtaggcgcca cccttgttca ctcgctctaa tgtcagtaat gccatatatc
    25801 gtactgattt tttcattttt ttagggattt ttttcattat ttcacctgct gaccaatctg
    25861 aacttgctga ccgcttccgt taaggaaatc atgtacggcc tgttttcctt taccagctgg
    25921 ctgtaattca ttgatggcta agacggtctg tttaccgcaa gcaatccata gatttttttt
    25981 gtctttccgg atgatcgtac caggtgcttt gtctgtagtt tctgccaata cttcaaccga
    26041 taataatttc cagcgtatgc cctcataggt cgtgaatgca attggccaag gacgcattcc
    26101 gcgtacttgg ttgtcaattt cttctgcagt tttatgccaa tcgattgctt cttgttcccg
    26161 tgtgatgttt ggcgaaaaag ttgcttctga ctcgtcttga ggtactggtt tttgtccatc
    26221 caaaatattg ggcaatgttt ctagcaatag ctttcttcct aaaaggctaa gcttttcaaa
    26281 catcgtccct acatcatctt gtttagtgat tggtatgctt tcttgcgcat agattccgcc
    26341 tgcatccatt tttttgatca tttccatgat cgttacacca gtttctttct ccccattcat
    26401 gatggcataa tgaacaggcg cacctccccg atatttagga agtaaagaag catggacatt
    26461 gattgcccct aattttggta cttgcaataa cttttcaggc aagaattggc caaatgccgc
    26521 agtgacgatc acatcaggca ccaattcaat gatttcttcc atttctggcg aaccggagat
    26581 tttctctggc tgcagcacac ggataccgtg cttcagagcc gcttctttta ccggtgtcgg
    26641 cgtaattact cgtttccgcc caactggacg gtcgggctgt gttaccactg cctggatctc
    26701 gtaacctgat tctatcagcc cttcaagaat cggcacagaa aaagcaggtg tgcccataaa
    26761 tactagctta gtcatcaaga tgttcctcca tatattcttc aagttcttct tccgggatct
    26821 gctgtatcat tttctcaatg aacaagatac cgtctaagtg atcgatttca tgctggaagg
    26881 cacgggctaa atagccaaaa gccgtcactt ctatctcttc tccgtctcga tcataataac
    26941 gcacggtcac ttcatctgct cgcttgaccg ttccatagac atgcggaata ctgagacaac
    27001 cttctacgtc aaggctttca ccttttgctt cgatgatttc tgggttaatc agttcaaatt
    27061 tctcgccttc atctacttcg atcacagcaa ttctcttgtt ttgtcctact tgaggagcag
    27121 cgatccctat tccgtcattc gcaatcattg tctcatataa attgtctaat aaagcgattg
    27181 tctcgtccgt gattacatcg attggctgag cagttctttt taatttatca ttagggtgga
    27241 tcaatatagg atagcgcata atttggccct ttcttaaata aagtttaatg gttcgttgtc
    27301 aattgccaca aaaagacctt gtcgctgttc tttctggctt ccattcaaaa tttcatccaa
    27361 aacatgagga agattcggtt catgtttata tttgatgatt agttgataat aatatcggtt
    27421 tttcactctc aaaatcatgc tgggcgttgg ccctaatagc aagctttgag ggcttaacac
    27481 ttcttttagc tgattggcaa tctgaaagat tttctttgcc gctacttgtt cttcaggatg
    27541 gctggcggtt attttcacag taaagtaata aggaggatag cctccacgat gccgtacatg
    27601 catttcctgc tgataaaact gctcataatt ttgtttttta gctaattcta tggcgtaatg
    27661 ttgaggattg aatgtttgga tcacgacttc gcctgctttt tccgctcgcc ctgctcgtcc
    27721 tgaaacttgg gtaagcaatt ggaatgtatg ctcactagaa cgaaaatccg gtaaattcag
    27781 ggaggtatca gcattcaaca ccccaactaa tgtcacttct ggaaagtcca aacctttagc
    27841 aatcatttgt gtccctaata atatatctgc ttcttttgca ccgaattttt gtaaaatctg
    27901 ttcgtgtgcc ccttttcttc gagtggtatc tacatccata cgcaaaatcc tagcttgcgg
    27961 ataaagttct cggagttcct cttctacttt ttgagtacct gttccatagt agcggatttt
    28021 attgccgcca caattaggac agcgattggg aataggttct tcatgtccgc aataatggca
    28081 gcgcattgaa cgtgtatcca tatgcagtgt aagagagata tcgcaattag gacatggaag
    28141 aacaaatcca cagtccctac acatcacaaa agacgaatat cctcgacgat tgagcagtag
    28201 gacagtttgt tcttttttgt ctaatcgatt ttgtattttt tcctgcaaat tagcagaaaa
    28261 agtagaggtc cgatgattct gaaactcttc acgcatatct acgatctcaa tagctggaag
    28321 ctgtgcagct gctttggctc ggtggtttaa ctgcaataac tgataaacgc ctttttgagc
    28381 tcgtgctctt gattctaaag agggcgtcgc gcttcctaga actattgggc aatgatgata
    28441 tttactgcgc caaattgcta gatctctcgc atgataacgc ggtgtctcat cttgtttgta
    28501 acttgattca tgttcttcat cgataatgat cagcccgata tttttcagcg gggcaaaaat
    28561 agcagatcgt gcgcctacta ctacttgcgc ttcttcccgc tcaatcttgc gccattcatc
    28621 atatttttca ccttgggaaa gtccgctatg taagaccgca actgattctc cgaagcgtcc
    28681 tttgaatcgc tcgaccattt gcggagtcaa tgcgatttca ggcactaaca taatagccgt
    28741 tttattttct gacaaaacat cagcgattgc ctgaagatat acttctgttt tcccgctgcc
    28801 agtgattccc tccagcaaaa agacctgatc tttttgctgc tgacctgctg tgatgatttg
    28861 ttcgacagca tttttttgtt ctgcattcag ttccaatgcc gtcgtttggt caaatactcg
    28921 gtctttataa gggtcgcgat aacgttctga ttctataaac tccagccagc ccttttcttt
    28981 tccttgattc aagacagcag tactgattcc tttatttttg aaataagcaa ttggttcttc
    29041 ttggctcagt tcagataaac acaaaagaag ttctttttgt ttcttggcat tttgccgtaa
    29101 ttttgcccat tcttcttcga tttgcttttc agttaacaaa gaacggacca aacgaatcgt
    29161 ttttacttta tttcgtgtat ggacttcata tttgatatct actaattgct cttggcgcca
    29221 gcgaatcaat tgagaaaggc tgccttcttc ttctgcttcc ttccaggaaa tcacttctcg
    29281 ctgatcaaac aatacctgaa cttctggtgt gtctgccaaa ggatagatca atttatcata
    29341 ttctgctttc atcacactcg gaagcatcgt ttgtagacaa gtgattttga atgcgtaagt
    29401 aatttttttc atataatcag ctaatgaaag taattcttca ttgaccaccg gagcaaggtc
    29461 tagcaaacga ataactgcct tcaaagatgg attagctgat tcttctgatt tttggatagc
    29521 cataacaaat ccttggacat gacgattgcc attaccaaaa gggacttcta ctcgcatccc
    29581 gacttgcaaa gctgtttcca tttcagaggg aaccaaataa gtgaatggct gatcggtctg
    29641 catagtaggt acgtccacaa tgacttcagc tgttaatgtc atggttcgtt tcctcctttt
    29701 tttgtctttt cttatcaagc gacaaaaagt tccttgccta ttttactaga gatcgatgga
    29761 tttgtcttat cttctgccaa atcctaacaa aaaattaaca gcagataaga aaaaaaagaa
    29821 ctgtgtcaaa aagttcagac acagcctttt ctaccagaca ataagtggcg aatgtttgaa
    29881 aaggaaacag ctgggtttgc ttttcttttt agatgttcgc tctatttttc tttattacat
    29941 tttttgctct tcacggatac gtgattccaa ttcgtgttgt tcacgctctt tttgtgcttg
    30001 acgttcctct tcttccatct tcaaacgttc acgttttaat tcaggatgtg gatcattgac
    30061 tacatttcct gcatcgattt cttctaaagc ttgaccgacg ctttttactg aatcaaaaga
    30121 atctagagta ggctgtgcac ttgcatccaa ttcatgcgcg cgtttacttg ctaaaatcac
    30181 tagagaatat tttgaattta cacgatctaa tagtgagtca attgaaggtt ttaacatcat
    30241 aagactacat ctcctttaac attttaatat atttaccgat tacgcgatct actcgaaaat
    30301 gttcactggc aataatgttt ttgattcgtt caactgctag cggcacttca tcatttacaa
    30361 cagcatagtc gtaaagagcc atcatttcga tttcttcttt tgcaactttc attcgttctt
    30421 cgattacttc atctgcatct gttcctcggc ctacgatccg tgattttaat tccgctaaat
    30481 ctggcggagt caaaaagatg aatacaccat cgggcacttt ttctttgact tgttttgctc
    30541 cttggacttc aatctccaag aaaacatctt ttccttcatc caatgtcttg ttgatatatg
    30601 acaaaggagt tccataataa ttcccgacat attctgcgta ttccagcatc tctccagctt
    30661 cgatcattga ttcaaactct tctttgcttc ggaagaaata atcaacgcct tcgacctccc
    30721 cttcacgcat tttacgggta gtcattgaga tcgaatactg aaaatcatta tcctcactct
    30781 caaaaattgc ttttcgtact gtgccttttc ctacaccgga tggaccagac aacacgatta
    30841 ataatccacg ctctgacatg acgacgtcct ttcaactagt ctttttgtac ttatcgttta
    30901 ttttgcactt tttttctgca actttcaagg ttatctttaa ttatagctag aaattggtaa
    30961 aaaagctacc ctgtaccaag aggtcgtaaa aaagctgtcc tgcatcaagt aataagaaca
    31021 gccaaacata aacagcttct catgtttttc gtttggttgg ggttattatc gcagatgcaa
    31081 gcttttgaac gccgtgtata agaggtcgtg aagaagccga ttagtttcga gtatgaagaa
    31141 aaattttgtg aaaatagttt ctcacatttt tgcataaatt tggcttaata
//
LOCUS       DACCQI010000019        30994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCQI010000019 DACCQI010000000
VERSION     DACCQI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.019"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(23..2095)
                     /locus_tag="IVO76_000673"
     CDS             complement(23..2095)
                     /locus_tag="IVO76_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7428660.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IVO76_000674"
     CDS             complement(2028..2405)
                     /locus_tag="IVO76_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7428661.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IVO76_000675"
     CDS             complement(2534..3094)
                     /locus_tag="IVO76_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7428662.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IVO76_000676"
     CDS             3244..4536
                     /locus_tag="IVO76_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7428663.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IVO76_000677"
     CDS             complement(4596..5714)
                     /locus_tag="IVO76_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7428664.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IVO76_000678"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IVO76_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7428665.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IVO76_000679"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IVO76_000679"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7428666.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IVO76_000680"
     CDS             8920..9519
                     /locus_tag="IVO76_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7428667.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IVO76_000681"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IVO76_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP7428668.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IVO76_000682"
     CDS             complement(11214..11558)
                     /locus_tag="IVO76_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7428669.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IVO76_000683"
     CDS             complement(11695..12831)
                     /locus_tag="IVO76_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7428670.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IVO76_000684"
     CDS             complement(13020..13994)
                     /locus_tag="IVO76_000684"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7428671.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IVO76_000685"
     CDS             complement(14074..14949)
                     /locus_tag="IVO76_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7428672.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IVO76_000686"
     CDS             15079..16986
                     /locus_tag="IVO76_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7428673.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IVO76_000687"
     CDS             complement(17527..20805)
                     /locus_tag="IVO76_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7428674.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IVO76_000688"
     CDS             complement(21076..21621)
                     /locus_tag="IVO76_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7428675.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IVO76_000689"
     CDS             complement(21633..22325)
                     /locus_tag="IVO76_000689"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7428676.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IVO76_000690"
     CDS             complement(22344..22628)
                     /locus_tag="IVO76_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428677.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IVO76_000691"
     CDS             complement(22641..23204)
                     /locus_tag="IVO76_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7428678.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IVO76_000692"
     CDS             23432..24094
                     /locus_tag="IVO76_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7428679.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IVO76_000693"
     CDS             24218..25414
                     /locus_tag="IVO76_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7428680.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IVO76_000694"
     CDS             complement(25515..26891)
                     /locus_tag="IVO76_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7428681.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IVO76_000695"
     CDS             complement(26928..27794)
                     /locus_tag="IVO76_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7428682.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IVO76_000696"
     CDS             complement(27883..28908)
                     /locus_tag="IVO76_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7428683.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYRLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IVO76_000697"
     CDS             complement(28926..29600)
                     /locus_tag="IVO76_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7428684.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKP
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29781..30677)
                     /locus_tag="IVO76_000698"
     CDS             complement(29781..30677)
                     /locus_tag="IVO76_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7428685.1"
                     /translation="MKKRTGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     RNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYWKFKRQKQLRKNNRS"
     gene            complement(30751..30876)
                     /locus_tag="IVO76_000699"
     CDS             complement(30751..30876)
                     /locus_tag="IVO76_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7428686.1"
                     /translation="MKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKFKMKSEV"
BASE COUNT         8953 a         6632 c         5548 g         9861 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgcggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg tcaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta ttattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaacg cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtatc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttcctttcct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgtttg cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacggaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggaatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgtgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacaatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gcctatattc ttggatgagg tagtcccgcg aaaaactgtc
    28381 tagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgttcg tccaccgctc aaatagtttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaaggttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgctt ttgtcttttc aatacagctt aaatttatta
    29761 tgttattctt aacaattggt ctatgatctg ttatttttcc ttagttgttt ttgtctttta
    29821 aatttccaat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgccttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctgattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagattgct ccgatcatat gttgttcgta gtattctttt
    30361 cctggatttc ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctgttctct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aatcggtttt
    30721 ataaaggttg ctgcagctta tttttgttgc ttatacttca gacttcattt taaatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataa
//
LOCUS       DACCQI010000020        29358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCQI010000020 DACCQI010000000
VERSION     DACCQI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.020"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            85..972
                     /locus_tag="IVO76_000700"
     CDS             85..972
                     /locus_tag="IVO76_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7428687.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IVO76_000701"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IVO76_000701"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7428688.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IVO76_000702"
     CDS             2274..2720
                     /locus_tag="IVO76_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7428689.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IVO76_000703"
     CDS             2993..4345
                     /locus_tag="IVO76_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP7428690.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IVO76_000704"
     CDS             4516..4803
                     /locus_tag="IVO76_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428691.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            5029..5472
                     /locus_tag="IVO76_000705"
     CDS             5029..5472
                     /locus_tag="IVO76_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7428692.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(5478..6662)
                     /locus_tag="IVO76_000706"
     CDS             complement(5478..6662)
                     /locus_tag="IVO76_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP7428693.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            6930..7628
                     /locus_tag="IVO76_000707"
     CDS             6930..7628
                     /locus_tag="IVO76_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7428694.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            7635..8312
                     /gene="nth"
                     /locus_tag="IVO76_000708"
     CDS             7635..8312
                     /gene="nth"
                     /locus_tag="IVO76_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7428695.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(8438..8971)
                     /locus_tag="IVO76_000709"
     CDS             complement(8438..8971)
                     /locus_tag="IVO76_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428696.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(9268..11649)
                     /locus_tag="IVO76_000710"
     CDS             complement(9268..11649)
                     /locus_tag="IVO76_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7428697.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IVO76_000711"
     CDS             complement(11639..12286)
                     /gene="recU"
                     /locus_tag="IVO76_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7428698.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            12350..12889
                     /locus_tag="IVO76_000712"
     CDS             12350..12889
                     /locus_tag="IVO76_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7428699.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            12979..13398
                     /gene="gpsB"
                     /locus_tag="IVO76_000713"
     CDS             12979..13398
                     /gene="gpsB"
                     /locus_tag="IVO76_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7428700.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            13420..13787
                     /gene="rnpB"
                     /locus_tag="IVO76_000714"
     ncRNA           13420..13787
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVO76_000714"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13996..15162
                     /locus_tag="IVO76_000715"
     CDS             13996..15162
                     /locus_tag="IVO76_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7428701.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            15208..16704
                     /locus_tag="IVO76_000716"
     CDS             15208..16704
                     /locus_tag="IVO76_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7428702.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            16785..17468
                     /locus_tag="IVO76_000717"
     CDS             16785..17468
                     /locus_tag="IVO76_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7428703.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            18113..19303
                     /locus_tag="IVO76_000718"
     CDS             18113..19303
                     /locus_tag="IVO76_000718"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7428704.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            19405..20358
                     /locus_tag="IVO76_000719"
     CDS             19405..20358
                     /locus_tag="IVO76_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7428705.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            20427..21776
                     /locus_tag="IVO76_000720"
     CDS             20427..21776
                     /locus_tag="IVO76_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7428706.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(21959..22282)
                     /locus_tag="IVO76_000721"
     CDS             complement(21959..22282)
                     /locus_tag="IVO76_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428707.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            22411..23574
                     /locus_tag="IVO76_000722"
     CDS             22411..23574
                     /locus_tag="IVO76_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428708.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            23644..24993
                     /locus_tag="IVO76_000723"
     CDS             23644..24993
                     /locus_tag="IVO76_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7428709.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(25038..25469)
                     /locus_tag="IVO76_000724"
     CDS             complement(25038..25469)
                     /locus_tag="IVO76_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7428710.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            25857..28499
                     /gene="alaS"
                     /locus_tag="IVO76_000725"
     CDS             25857..28499
                     /gene="alaS"
                     /locus_tag="IVO76_000725"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7428711.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            28699..29295
                     /locus_tag="IVO76_000726"
     CDS             28699..29295
                     /locus_tag="IVO76_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7428712.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
BASE COUNT         9822 a         5066 c         6139 g         8331 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg
     4981 aataggaaac aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga
     5041 caactgctaa tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca
     5101 gaaatggaaa aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc
     5161 gggaaaatcc ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga
     5221 gccatcagta agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc
     5281 gaagagaata aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat
     5341 agacacgagc aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg
     5401 tcaaaagaag aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga
     5461 agtagcaaat gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa
     5521 aatccgaata ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg
     5581 ccgatgacgc gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg
     5641 ttatctgaga cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg
     5701 aaaccataac agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat
     5761 gtgatgaata gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct
     5821 gaaaattttt tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca
     5881 atcaaagaga agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat
     5941 tgagaaaaga tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat
     6001 aaaggaataa cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg
     6061 gagaaactta aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag
     6121 ccataaacgg catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc
     6181 aactcagcta atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca
     6241 cccatccatc caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca
     6301 tataaggtgg agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc
     6361 catatcgaat ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata
     6421 ataaggatcg gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag
     6481 aaggcagcta gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg
     6541 gagtgagaat aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat
     6601 ccagtgaata agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac
     6661 atgtaaaaac tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt
     6721 ttgtttcctt gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa
     6781 agtgaagata cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc
     6841 tgaccgatca gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac
     6901 gtatactgtg attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg
     6961 gacaaacagt catatctaat cttttactta aaaattatca taggatcggt ctacaatctg
     7021 aagaattttt atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg
     7081 atttgcagat gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg
     7141 atcgtctcgt aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag
     7201 cagatcgata taatctttac cccatctatg atctattagg tgagtattta aaaacacaag
     7261 aaaaaaaata cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg
     7321 aacaagaatt tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag
     7381 aagaagacca ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc
     7441 aggcttatag ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac
     7501 gtacaaaaca acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag
     7561 aagtagaaca gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg
     7621 aggagtagcg gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg
     7681 gaatgtttcc cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg
     7741 cagtgatctt aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt
     7801 ttgcctcttt tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta
     7861 aaataaaaac gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc
     7921 agctgattga acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc
     7981 ctggtgtagg cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa
     8041 tcgcagtaga tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg
     8101 ctacggtcat ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa
     8161 agacacatca tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt
     8221 gcgaggtatg tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag
     8281 aaaaggcgct aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg
     8341 tatgaattat cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa
     8401 gtaaatgtac atactcagta gctaggatgg taacttatta caatttattg cgataaaaat
     8461 aaaagttgtt atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac
     8521 ctataaaata agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag
     8581 gtgtaataaa aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa
     8641 tggatttggg cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata
     8701 aaaaagtgag aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc
     8761 ttaaaagtac agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta
     8821 ataataaaca aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata
     8881 aaaaaagatg gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga
     8941 agccttcttt caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa
     9001 tgggattatt aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta
     9061 taaacaagta aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt
     9121 taagctaaat gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat
     9181 acagcttggt acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc
     9241 acgcttgtag acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga
     9301 tggtgtatgg tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg
     9361 aggagcagat gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga
     9421 agacgctggc tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc
     9481 gctttgacta gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt
     9541 ctggacatag ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt
     9601 atattgatct ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca
     9661 gtctgtattt gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc
     9721 tacgtgtgac gattctgaag tgacaggtgt cattttttta ttgtaacctg tccacacgga
     9781 aatcgaataa tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat
     9841 accaagtttt gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata
     9901 taacccagcg atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc
     9961 tgtgatcata taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc
    10021 ttctgttccg tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc
    10081 attggcgaaa gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc
    10141 atcttgttct tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat
    10201 cccaagattt tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac
    10261 tgctggaaca ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg
    10321 attatcccag tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat
    10381 agtttttcca gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt
    10441 catcgtagaa ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc
    10501 ttctgcgatg tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag
    10561 ggtggaggca acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat
    10621 atcatatagt ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt
    10681 gtagacattc ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc
    10741 gacgatctta gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac
    10801 attaacagct tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa
    10861 aacagtatct cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg
    10921 ggcttgaggc atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt
    10981 accaaagtac atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt
    11041 atttacgtag taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat
    11101 ccacgcttct tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga
    11161 aagtttgatc aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt
    11221 agagagggca gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg
    11281 atcttctacc gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt
    11341 ttcacgcttt tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga
    11401 agatacggta gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca
    11461 gaaaagtcct acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat
    11521 cttgataaat aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt
    11581 cttcttcaca ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc
    11641 atttgccatt cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt
    11701 ccaaataagg aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca
    11761 aagataaagg tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat
    11821 gaacactatt caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga
    11881 ccccattttg ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa
    11941 agggaaaaga tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat
    12001 agacgccatt atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag
    12061 cacttctctt aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa
    12121 ccgccaaccc gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca
    12181 tcccccgatt gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag
    12241 aggcattctt ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc
    12301 ctaggccaca ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc
    12361 aaaagtaatc tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga
    12421 tcctaaaatc caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga
    12481 agggctggaa tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat
    12541 agtagcggaa ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc
    12601 tgattttggt aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt
    12661 agcagattat gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa
    12721 tcacactcgt tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt
    12781 ccctggaaaa acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta
    12841 cgaaattcgg ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg
    12901 tttgatttat caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg
    12961 aaaactagag gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa
    13021 gaattcaaaa caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat
    13081 atcatcaaag attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat
    13141 cgattgagtg caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg
    13201 caacaaacag aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg
    13261 tcaaatcttg aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa
    13321 ccagcacaac ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa
    13381 aaaacacgtc aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct
    13441 gacttgatca ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt
    13501 gcttagcgaa accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg
    13561 cttcggctat gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc
    13621 atccggtgga acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt
    13681 ttgtctggaa gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt
    13741 tgttttttct ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc
    13801 agggaattag gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc
    13861 tatttttcaa tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt
    13921 tattcggaat ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa
    13981 ataaggagat tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc
    14041 gggctagaag cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag
    14101 aacggacgtg cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta
    14161 cgtacagcag atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa
    14221 ttgtttgaaa acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt
    14281 cctgtagctg gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc
    14341 acaaaaaaag caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca
    14401 ctgacggaaa ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg
    14461 gtaactctgg atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt
    14521 ggagcaccat tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa
    14581 gaccgtccat tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta
    14641 ataggccata atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg
    14701 ttcagtcaag aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat
    14761 attgaattag atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc
    14821 aatgcagaag aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac
    14881 ttcaaaacgg ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta
    14941 ggagaagaag aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta
    15001 aaaacatgga gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa
    15061 aaagcaacca aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat
    15121 tggggaactc gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat
    15181 tcaattggat gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa
    15241 gaaatcaatc ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg
    15301 cctgaaaaag ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt
    15361 tcaaaaaaaa tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa
    15421 gaattgtcgg aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa
    15481 catcgcgtac cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga
    15541 caatggcaaa aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa
    15601 aatattgaat taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat
    15661 gatgttttgc tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt
    15721 gatcagctta aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa
    15781 cctgaaacag actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg
    15841 aaagtaatcg aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac
    15901 ccatttatga ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca
    15961 gatttcaaaa tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa
    16021 gacatcgatg ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat
    16081 gaatcacaat cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag
    16141 caatatcctt tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa
    16201 ttttatcgtt cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg
    16261 acttaccctc tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg
    16321 gtagatgttg ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata
    16381 cgaccaacaa atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt
    16441 ggttattttc cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg
    16501 cagcaagaaa tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac
    16561 tggatgtcag agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg
    16621 gatgcaacac atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat
    16681 tttaatgtat atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat
    16741 ctatagatgt tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag
    16801 tgatcgctga ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc
    16861 ataccaaaag agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc
    16921 gctttgtgac gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa
    16981 atgagaacga ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg
    17041 aacagatctc gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg
    17101 aagcggccga actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat
    17161 taggagcaat cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt
    17221 ttgatcctga actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga
    17281 cagtgatcaa tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt
    17341 acggatatga agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt
    17401 ttctggaatg gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca
    17461 aaaaatgatg gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg
    17521 taaaagttac ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga
    17581 gtcacaagga acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga
    17641 ttcggaattg gcaggagaac accgtttatt cggaagaata ggactgtgac aagacttttg
    17701 tcacagtcct tttttcgaat tcaacaagta aaacttttaa gaaatattca gagagtaaaa
    17761 ttaaaaattg ctgataaacg aagatggaaa ttttatgtat ttttattggt ctatgcaata
    17821 aataaattaa atattcagaa tacaattgat tttaacgaaa gggcttctat caatggttgt
    17881 gttgtattta tctgcccagg tagagtaaaa tagatcatta tctaaaaggt aattgttcat
    17941 actatataag tcatttgaaa gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt
    18001 ctcataatca gtgtaagcgt tatcaaaaat atagcatact tgctttgtag atgaatgaca
    18061 cgatatatgt ttggtatgtt ctgcaagaca gatgaatatg gaggtggaga caatgtcttc
    18121 caaagaacta acaatgtatt tagagaaaga attggatcaa ctgaaagaaa aggggttata
    18181 caatacgatc gacatactag aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa
    18241 gatgatcaat ctagcatcca acaattatct tggatttgcc aatcgcgaag aattgaaaaa
    18301 agcatgtatt gaagcaaccg aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa
    18361 tggttcgctg aaaatccatc agcaattaga agaaaaaatc gcagaattca aagggacaga
    18421 agcagcaatc gcctttcaat ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat
    18481 gacaaaagaa gatgctatct tatcggatga attaaaccat gcatctatta ttgacggttg
    18541 ccgcttatcc ggagcaaaaa tcatccgaat taaacatcaa gatatgaaag accttgagaa
    18601 aaaagcaaag gaagcgatag aaagtaagaa atataaaaaa atcatgtata tcacagatgg
    18661 tgttttctcc atggatggag acattgcacg attgccggaa attattccga ttgttgaaaa
    18721 atatggactg atcacctacg tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc
    18781 aggaaccgtc aagcattttg gattaagcga caaaatcgat atgcaaatgg gcacgttatc
    18841 gaaagctata ggtgttgttg ggggttatgt agctggatcg aaaacactaa tcgactggtt
    18901 gaaagctaga agtcggccat tcctattttc tacttcgcta actccaggtg cagctgcagc
    18961 tgcattggct tcgattactt tgatgcaaga acatccggaa cttgtcgaaa aagtttggga
    19021 aaatgctaat tattttaaag aagaattgaa aaaagtaggc tataacatcg gtatgtctga
    19081 aacacctatt actccagtca ttttgggaga cgaaaaagtg actcagactt tctctaagaa
    19141 gctgatcgaa catgggattt atgctaaacc aatcgtttat ccaactgtgc ctcttgggac
    19201 aggcaggatc agaaatatgc cgactgcgga acatacgagg gaaatgcttg atgaagcagt
    19261 agcagtttat caaaaaatag gaaaagaaat ggaaattatt taactaaatt tagttttttc
    19321 gtctatttaa aattagagaa catgttcacg catatattgt taatcatctg aagatagtag
    19381 taaaaaagat cagggaggat catcatgaaa aaagtattgg tgacaggatg tttaggacaa
    19441 attggttcag agttgatcac acggctacgt caagaactag gcagtgaaaa tgtcattgcg
    19501 acagatatac gcatgcctga agtcattgag aaaaacgata tctttgagat cctagatgta
    19561 atggattacg aaaaaatgcg cagcatagca gaacaatatc aagtggacac attgattcat
    19621 ttggctgctc ttttgtcagc tgttgcagaa gcaaaaccac aattagcctg gaatttgaac
    19681 gtgactgggc tggtcaatgc attggaagtc gctagagaat tccagctgaa attttttacg
    19741 ccaagttcaa tcggcgcgtt tggtccaaat actccaaaag atcatacccc acaagatact
    19801 cttcagcgtc ctacaaccat gtatggggta acgaaggtag ctggagaact actatgtgat
    19861 tattattatg tgaaatatgg cgtagatact cgtggtgtcc gtttccctgg attgatttct
    19921 tatgcaacct tgccaggagg cggtacgaca gactacgcag tcgatattta ttattcagca
    19981 atcaaagaag gaaagtacac tagtttcatt gatcaaggta catcaatgga tatgatgtat
    20041 atgccagatg cgatcgatgc cattataaaa ctcatgaatg cagatcctga tcgattggtt
    20101 catcgaaatg ccttcaatat atcggcgatg tcttttgacc cggaagagat caaagcatct
    20161 attcaaaaaa tcataccaga ttttgaaatg gattatgatg tggatcctgt acgtcagtca
    20221 atagcagaat cttggccaaa ttcactagat gtttcttgtg cgcaagagga atggggcttt
    20281 tcgccgaaat acgacttaga tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa
    20341 ttggtagaga cggtataaaa caagaaccac atccaaaaag ttttttatac ttttgggatt
    20401 ggctatggta tgaaagggga caagggatgg aagaatatca agccacgcca gtaaaagaag
    20461 taaaaataga aaatgaatta aaacgtacga tgggattttt tactgcttta tcgactgtta
    20521 tgggcacagt gatcggtgca ggggtatttt ttaaagccgc aagcgtagca gaagttacag
    20581 gatccgccag tttgcatatg ttttcttggt ttctcggtgg tatgatttct gtctgtgcag
    20641 gactaacagg agctgaactg gcagctgcta ttccagaaac aggagggatg attaaatata
    20701 tcgaacggat ttacggaaat acagcagctt ttttattggg ctgggcgcaa gtcgtgattt
    20761 attttccagc aaatgtagca gccttgtcga tcattttcgg tacacagttc gtcaatctat
    20821 ttggtctttc ccagtcaatg attgttcccg tagctgttac agctgctgta tcgatcctgt
    20881 tgatcaattt tttaggttct aaagctggag gggcttttca gtcgattacg cttgtctgca
    20941 agcttattcc tttgtttgtg atcgtcattt tcggtttgtt ccgacaagaa ggagtagact
    21001 tccaactgtt tccgatccaa gcaggagaaa atctttcatt tttctcagcc ttgggagcgg
    21061 gcttgctagc aacgatgttc gcatacgacg gttggataca cgtaggaaat atttcgggag
    21121 aattgaaaaa gccggcgaaa gatttgccaa aagcaatttc tttaggaatc atcggaatca
    21181 tgatcgttta tttattagta aatgcggtgt tcttaagaac cgcatctatc gacggagtcg
    21241 caggaaacag caatgcggca agtgacgttg caaaaatgat ttttggcggc ttcggtggaa
    21301 gactggtcac agttggtatc ttgatttctg tttatggaac catcaatggc tataccctta
    21361 ctggaatgcg tctcccttat gttatggcaa aagaaaacaa cctgcctttt agcaagctct
    21421 ttgctaaact tcatgataaa actaaagttc ctgtagccgc aggtatctta gagctagtca
    21481 tcgctattgg catgatgatg atcggtggtt ttgatacact aacggatatg ctgatctttg
    21541 tcatttggat tttttatacg atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac
    21601 ctgatttgtt tcgaccatac aaagttccaa tgtatccgtt tattccacta gtagcaatca
    21661 ttggagggac ttttatcgta tcaagcacgc ttatcactca gaccttttta gcttcgatgg
    21721 ggatagtatt gacacttgct ggtatcccga tttatctcta tttaaaacgt agatagctaa
    21781 aacattcgaa ttttattaga actgcacttc tctgccaaac aaaatatttt atttaaatga
    21841 ggagtacgtg gaagaaatgt caaaagtact cgttaatttt gatcacgtaa atgtcgtttt
    21901 gtctacgatg aaatagcaaa gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt
    21961 actttgattt gcgtaactga actattcggc gtacgccgcg ataaatattt aataaaacca
    22021 agcaaccaga aatgaaaatg ggaagccaaa tcagatagac agggtagcca agcactttag
    22081 acaaagcatg tggagtcatt ttatccattt caagaagtgc ttgctgccaa gtctgaagat
    22141 tgaagactag catgaaaccc gaaataatca ggagaataaa tcctaaataa tgaattgtag
    22201 ttctttttct tacataagcg atcgtagcat aaaggaacag taaaaaaata ggtacgccaa
    22261 aaacaaagta catattcagc atctcgacac cccctttgtg tttatcttac tttactttga
    22321 aagaaatgag aaataatagc ctcgttactt gcttgaaaca gcaaaggact gctaagataa
    22381 gagagataca aagaatcaag gaggagtcat atggcaagtt atatcgaacc gattcgtttt
    22441 gcgctgattg tttttccatt tttagcctta gcgatctcat ctgttttttt tatttatgaa
    22501 tatcgaaagt atggaacatt tctagtaaca agggcagtta ttttgtattc tttcgttttt
    22561 tatttattgt gtgcgtattt tttggtgatc ttaccacttc cggcaaagga gacggttgcc
    22621 caaatgacag ggccaaagat ggagctgcat ttatttgctt catggcaaca tttcagagac
    22681 aacacggtat tggtcttgtc tgaacctagt acctacctgc cggcaatgaa gcagagtgta
    22741 tttttggaac cagcttttaa tgttttatta ctcataccat ttggcattta tttaagatat
    22801 tatttccggc tttcttttat gaaaacgatg attgctagtt tctgcctttc tctatttttt
    22861 gaattgacgc agctaagtgg actttatttt atctacccaa gaccataccg cttatttgat
    22921 gtgaatgatt tagtgacaaa tacattgggt ggaatcattg gttttgtcgt tacgccgtta
    22981 tttacattta tgcttcctac aagaagacga atggatgaac tttcttatga aaaaggaaag
    23041 actgtctcat tgactcgacg cttggtcgct tatttgatcg actggacatt tttatctctt
    23101 attaatttgg tagcggcgat tttctttaaa attgcgttcg attcaggggc agttacttct
    23161 catatctggt ggcttttatt agaagtaggg gtatacttta tgattgtacc gtttataacc
    23221 aatggatgga cacttggcaa aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa
    23281 cgaatttctt taaaagcatt gatgatccgc tacgggtatc tgtactttct tttttatggt
    23341 ttaagctggt tggggcagtt gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg
    23401 cagatgagtg cagtattcat ttctctcggt gtcttgttac tacaaggtat ttttatttta
    23461 aatatcttgc tatcattttt gagaaaaaaa cggattctgt tttatgaaaa agctagtcac
    23521 acatatactg ttagcacgat tggccaaaca aaggatgatg attctcgaaa ttagctgagc
    23581 aaaaacgctt tgtctgtgtt ataatgccaa agtcgtgata atttaaagag aagaggtgca
    23641 cgaatgaata attttgatat attccgcttc aaaccgttta tcaatgaagc gttaaccgca
    23701 aagggcttca aacagcccac agaggtccag gaacgtttga ttcctttaat tgcaaaagga
    23761 aaaaatatca ttgggcaatc tcaaactggt acaggaaaaa cacatacgtt tttgttgcct
    23821 ttgtttgaaa aaatcaatgc agaaaaagag gaagtacaag ttgttattac agctccaagt
    23881 agggaactag ctgcacagat ctatgaagca gctacacaaa tcgctaaatt ttctacaccg
    23941 gagatccgtg tctcgaattt tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg
    24001 catcaccaac agccacaggt tgtagtaggc acgcctggta gaatattaga catgatgaat
    24061 gaacaagcgc tgaagattca caccgcattt gcatttgttg tcgatgaagc ggatatgaca
    24121 ttggatatgg gttttttaaa tgaagtagac caaattgcaa gtcgattacc agaaaagctg
    24181 cagtttttag ttttttctgc cactattcca gaaaaacttc gtccattctt acgaaaatac
    24241 atggaaaatc caatcattga agaaatcaaa ccaaaagcag ttatttctga agacattgaa
    24301 aattggctga tttcaacaaa aggtaaagat gagaatcaaa tgatttatca actgctgaca
    24361 attggtcatc cttatttagc aatcgtattt gccaatacaa aacagcatgt ggatgaaatt
    24421 gccgattact tgaaaaacca aggcttgaaa gtagcaaaga ttcatggaga catcacccca
    24481 agagagagaa aacgagtgat gcgacaagta cagaatctgg agtatcaata tgttgtagcg
    24541 actgatttag ctgctagagg gatcgatatc gaaggtgtct ctcatgttat taatgcagaa
    24601 attccagaag atctagactt ctttatccat cgtgtcggac gtactggacg taatggctta
    24661 aaaggaacgg ccattacgtt atattcgcca aatgatgagc aagccattga tgcgatagaa
    24721 aaattgggcg tctcatttga accaaaagaa atcaaaaacg gggaaatcgt caaaacatac
    24781 gatcgtaata gacgtgccaa acgagaaaaa tcaaaagatg tattagatcc cacgctgatt
    24841 ggtcttgtga agaagaaaaa gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg
    24901 gcaattgcag aaaaaaataa aaaagaacgc aaaatcgaac gcagacagca aagccgtact
    24961 gctcgtaaaa ataagaaaaa ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt
    25021 gtcacaatct cttgttttta agctagactt tttttcattc cgatacaaag tacttcatct
    25081 tcaataaaag gttctgtgca agtatgatac ccttgttttt cataaaaatc tacagccgtc
    25141 atttcagcta ctacataact agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct
    25201 tcaagcaact gaccgcctaa cccttttcct cggtattctt ttgctacgca aaatcgttcc
    25261 ggcttgaagc aagtaggtgt tttcttttca taacgcaaag tagctactgg tcttttttga
    25321 tcaagaaata gataataggt ttgttgcggg atatcttttc cgtcgaattc tagttctgca
    25381 ggaatccctt gttctaatac aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc
    25441 cattcatccg ctccaactat ttttttcatc atattcctcc ttgtcatgaa tagcagagat
    25501 gtattgattc ctttattctt atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga
    25561 aaataaaaat tgagatattg acagtgatca atagaatgcg tatactaatt aagtacgaaa
    25621 aggatatgat ttgtttttta agactcacag aaaattcttt cttggctgaa aaaagaatag
    25681 tcactaatca aattgctccc tttttctatt ttggtagaaa taccgacgca gctcagcgtt
    25741 atcgagttta agaggtaatg attacacatc attgcaatca aggtggtacc gcgagttttc
    25801 gtccttggct gtgatggtgt ttttttgtat ttaatttata gaaagcaggt aatgacatga
    25861 aacaattaag tagtgcacaa gttcgacaaa tgtttcttga tttcttccaa tctaaaggac
    25921 atacgattga acctagtgcg tctttgatcc ccgtgaacga tccaacctta ttgtggatca
    25981 actctggtgt tgcaacattg aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa
    26041 gaatcacaaa tgcccaaaaa tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag
    26101 cacggcacca tacgatgttt gagatgctgg gtaatttttc gattggtgac tatttcaaaa
    26161 atgaagccat ccactgggca tgggaatttt tgactgatcc aaaatggatg gcattcgatc
    26221 cagaaaaact ttatgtaaca gtttatccaa aagatacaga agcaaaacgc atctggcatg
    26281 aagaagttgg cctgccacta gatcacatta ttgatgtaga agacaacttc tgggatatcg
    26341 gtgcaggccc atgtggtccg gatacggaaa ttttctatga ccgtggagaa gcttataacg
    26401 atttagcaga agacgatcct gaaaactatc caggaggaga aaatgaacgt tatttagaaa
    26461 tctggaactt agtattttca gaattcaacc ataagcctga taacacattt gaaccacttc
    26521 ctcataaaaa cattgatacg gggatgggat tggaacgcat ggtctcaatc gttcaagatg
    26581 cgtcaacgaa ctttgagaca gacttgttta tgcctattat ccacgccgta gaaaagctaa
    26641 gcaaacaagt aaaatatggt gaaaacccag taacagatat ttcttacaaa gtaatcgcag
    26701 atcatattcg cgccctttca tttgctattg gcgacggtgc attgccatcg aatgaaggga
    26761 gagggtatgt cttacgccgt ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta
    26821 ttgaacaagc attcttatat cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt
    26881 atcctgaagt acttcaacaa aaagaattta ttgaaaaagt tatccgtaca gaagaagaac
    26941 gtttcaatga aacaatcaat gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa
    27001 aaaacgaaaa cgggacgact ctagcaggaa aagacatctt caaactatac gacacatatg
    27061 gcttcccggt agaattaacg gaagaggtgg cagaagatga aggactgaaa gttgatcatg
    27121 ctggttttga aaaagaaatg gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg
    27181 aaaaatcaat gggggtacaa tctgctttat tgacagatat caaagtagaa agtaaattca
    27241 ttggatacac agaacaagaa tcagatagtc aattattggt catcgttaaa ggtgagtctc
    27301 ttttgaatga agtcgcagaa ggtgaagctc aaatgatctt tgatcaaaca cctttctatg
    27361 cagaaatggg tgggcaagta gcggatcaag gatggatcat gaatgaagta ggcgaaatcg
    27421 tagcagttgt tacagatgtt caaaaagcgc ctaatggtca atttatgcac accgtagaag
    27481 taaaagcacc aatgagtgaa ggacaaacct atcatctatt gatcgatcaa tcgatgcgca
    27541 atcggatcat caaaaaccat acagcaaccc atctactgca caaggcattg aaagaagtac
    27601 ttggcgagca tgcaaatcaa gctggttctt tagtcgctcc tggacattta cgattcgact
    27661 ttacccactt tggtcaaatc acttcagatg aattagctga aatggaaaga atcgtcaacg
    27721 aaaaaatctg ggaagctatc ccagttgaaa caattgaaac agatatcgat acagccaaaa
    27781 acatgggtgc aatggctttg tttggtgaaa aatacggaca tgacgttcga gtagtaaata
    27841 ttggcggctg gtcaatcgaa ctgtgcggag gcacacatgt agcaaataca gaaaacatcg
    27901 gaatcttcaa aatcgtttct gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg
    27961 taacaagtaa agaagcgtac gaactacttg aagaagaaga aaaacaacta aaacaagtag
    28021 caacaattgt taaatcacct caattgaaag aagttgtttc aaaagcggaa caattgcaac
    28081 agcaagtaag agagctacaa aaggaaaatg aacaattagc tagcaagtta gcgaatcaac
    28141 aagctggtga tgtattcaaa gatccagaaa cgatcaatgg aaaaacaatc attgcagcac
    28201 aagtaaaagt gaaagatatg aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa
    28261 atatttcaga tattcttgtt ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg
    28321 caatgacgaa agaatcaaat gaagctggac taaaagctgg cgacttgatt aaagcaatcg
    28381 caccgaaagt aggcggtggc ggtggtggac gtccagacat ggcacaagct ggcgggaaaa
    28441 atcctgctgg tattccagat gcacttaaag ctgcgaaaga atgggttgcg caacaatagg
    28501 gcgtttacgt ttgacagaaa aacaaaagtt caaacattga acgaaacatg attatggggg
    28561 cgaagacaaa caagtcttgc cctcatactt ttttcaattg gatggcgcat ctaatcaaat
    28621 ggtcggattc ggatggtctt tagttgacga aagtgacaga aatttttacg attagtataa
    28681 ataaaaaggg gtgaaaaaat gaatagtaga aataagacct ttcgactagt cttacgtgcg
    28741 atcttgttag caatcatcat cgtacaagcg atggttcctt ggctggggtt tattccatta
    28801 ggattcatta gtttaacaat tatccatatc acagtaatta ttgctgcagt agtattaggc
    28861 ccaaaagatg gcatggtgat tggcttgttt tggggaatcg ctacaattgt tcgtgcctat
    28921 gccatgccaa caacgccatt tgatacatta gtgttcacga atccgattat ctcggtagtt
    28981 cccagagtac ttgttggctt agtagcaggc ttggttttcc actggattta tcaaaaaaat
    29041 aaatcaataa cgatcagctc agcctttgca ggggtactgg gttcattggt caatacagtc
    29101 ttagtgttag gattcatggg attattttat acaggtgcaa cggcagatgc ctatggtgta
    29161 gactctgctc ttttatttaa aaccttagca ggggttgcag caatcaatgg gataccagaa
    29221 gcaataggtg cgggaatcat aacaccatta ctagcaaaag cgttatttgc agcaacaccg
    29281 ttgaaaccag aataatgacc atcttttaat agtaatagga cgcacgatag atagaaaaag
    29341 gaagagagga gaggaaaa
//
LOCUS       DACCQI010000021        29220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCQI010000021 DACCQI010000000
VERSION     DACCQI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.021"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..600
                     /locus_tag="IVO76_000727"
     CDS             <1..600
                     /locus_tag="IVO76_000727"
                     /inference="COORDINATES: protein motif:HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428713.1"
                     /translation="ADAEFTLTDEKGVKEVKKTDATGLATFNIIANKTYNLEETKVPT
                     GYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQGKALANAEFTLTDDKGV
                     KVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEGITVADDGQTFEYTVINH
                     LVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            649..2484
                     /locus_tag="IVO76_000728"
     CDS             649..2484
                     /locus_tag="IVO76_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7428714.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            2559..3416
                     /locus_tag="IVO76_000729"
     CDS             2559..3416
                     /locus_tag="IVO76_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7428715.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            3406..4515
                     /locus_tag="IVO76_000730"
     CDS             3406..4515
                     /locus_tag="IVO76_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7428716.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            4854..5654
                     /locus_tag="IVO76_000731"
     CDS             4854..5654
                     /locus_tag="IVO76_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428717.1"
                     /translation="MVEISKTTLDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            5742..6548
                     /locus_tag="IVO76_000732"
     CDS             5742..6548
                     /locus_tag="IVO76_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428718.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            6780..7649
                     /locus_tag="IVO76_000733"
     CDS             6780..7649
                     /locus_tag="IVO76_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7428719.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            7669..8016
                     /locus_tag="IVO76_000734"
     CDS             7669..8016
                     /locus_tag="IVO76_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428720.1"
                     /translation="MEIISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            8029..9291
                     /locus_tag="IVO76_000735"
     CDS             8029..9291
                     /locus_tag="IVO76_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428721.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            9371..10747
                     /locus_tag="IVO76_000736"
     CDS             9371..10747
                     /locus_tag="IVO76_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7428722.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            10744..11373
                     /locus_tag="IVO76_000737"
     CDS             10744..11373
                     /locus_tag="IVO76_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428723.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            11392..11871
                     /locus_tag="IVO76_000738"
     CDS             11392..11871
                     /locus_tag="IVO76_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428724.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            12373..12426
                     /locus_tag="IVO76_000739"
                     /pseudo
     CDS             12373..12426
                     /locus_tag="IVO76_000739"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12430..13029
                     /locus_tag="IVO76_000740"
     CDS             12430..13029
                     /locus_tag="IVO76_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7428725.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIHFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            13045..13521
                     /locus_tag="IVO76_000741"
     CDS             13045..13521
                     /locus_tag="IVO76_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428726.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            13538..14440
                     /locus_tag="IVO76_000742"
     CDS             13538..14440
                     /locus_tag="IVO76_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428727.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQANNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            14513..14707
                     /locus_tag="IVO76_000743"
     CDS             14513..14707
                     /locus_tag="IVO76_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428728.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            14812..15240
                     /locus_tag="IVO76_000744"
     CDS             14812..15240
                     /locus_tag="IVO76_000744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428729.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNKETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            15475..16062
                     /locus_tag="IVO76_000745"
     CDS             15475..16062
                     /locus_tag="IVO76_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428730.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            16062..16961
                     /locus_tag="IVO76_000746"
     CDS             16062..16961
                     /locus_tag="IVO76_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP7428731.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            16961..17875
                     /locus_tag="IVO76_000747"
     CDS             16961..17875
                     /locus_tag="IVO76_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428732.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESGMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            17927..18142
                     /locus_tag="IVO76_000748"
     CDS             17927..18142
                     /locus_tag="IVO76_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428733.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANRLY"
     gene            18332..18586
                     /locus_tag="IVO76_000749"
     CDS             18332..18586
                     /locus_tag="IVO76_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAP7428734.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            18765..19814
                     /locus_tag="IVO76_000750"
     CDS             18765..19814
                     /locus_tag="IVO76_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP7428735.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            19993..20223
                     /locus_tag="IVO76_000751"
     CDS             19993..20223
                     /locus_tag="IVO76_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428736.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            20226..20483
                     /locus_tag="IVO76_000752"
     CDS             20226..20483
                     /locus_tag="IVO76_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428737.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            20700..21575
                     /locus_tag="IVO76_000753"
     CDS             20700..21575
                     /locus_tag="IVO76_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428738.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQENLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSEKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKEE
                     ITKMKPVIKILDNKE"
     gene            21585..22379
                     /locus_tag="IVO76_000754"
     CDS             21585..22379
                     /locus_tag="IVO76_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428739.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNNGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            22408..23526
                     /locus_tag="IVO76_000755"
     CDS             22408..23526
                     /locus_tag="IVO76_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428740.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKVKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTVNEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFAKSKDEVKGVVIDDDFEFDFD"
     gene            23559..23879
                     /locus_tag="IVO76_000756"
     CDS             23559..23879
                     /locus_tag="IVO76_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428741.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            24136..24825
                     /locus_tag="IVO76_000757"
                     /pseudo
     CDS             24136..24825
                     /locus_tag="IVO76_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
     gene            24862..25029
                     /locus_tag="IVO76_000758"
     CDS             24862..25029
                     /locus_tag="IVO76_000758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428742.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            25049..26677
                     /locus_tag="IVO76_000759"
     CDS             25049..26677
                     /locus_tag="IVO76_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428743.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            26728..27096
                     /locus_tag="IVO76_000760"
                     /pseudo
     CDS             26728..27096
                     /locus_tag="IVO76_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27196..27396
                     /locus_tag="IVO76_000761"
     CDS             27196..27396
                     /locus_tag="IVO76_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428744.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            27530..28756
                     /locus_tag="IVO76_000762"
     CDS             27530..28756
                     /locus_tag="IVO76_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428745.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
BASE COUNT        11247 a         3828 c         5868 g         8277 t
ORIGIN      
        1 gcagatgcag aattcacctt aacggatgaa aaaggtgtaa aagaagttaa aaagacggat
       61 gcaactggtc tggctacgtt caacattatt gcgaacaaaa cttacaactt agaagaaaca
      121 aaagtaccaa caggttacac aggcgcattc aaacaagaag gaattaccgt caaagatgat
      181 ggacaagtct ttgaatatac agctaaaaac actttgaaaa aaggtgtgat caaagttcat
      241 aaagtcgatg atcaagggaa agcattagca aacgcagaat tcactttgac ggacgacaaa
      301 ggtgtaaaag ttgtacaaaa aacggacaga aatggcttgg caacatttaa tattgttgcc
      361 aacaaaacgt acaaaatcga agaaacacaa aaccctaatg gttatacagg gaaatggtct
      421 aaagaaggca tcaccgttgc tgacgatgga caaacctttg aatatacagt aattaatcac
      481 cttgtacctg aaacagtaag aacaggcgga atcggtacat cagccttcct tcttgtattt
      541 ctaattgcag gattgagtat ggtgctactg tattatcgag aacagcacaa agcaaaataa
      601 aaaagtgtta atggaaaaga agtaaatatg aaaaaagaag gctgacgtat gaaatcaaaa
      661 aaaatttttt caaatttaat gctatttggt actttgctag gagtgggatt gggcgcaagc
      721 tcaatctctt ctagccttgg actagggggg caagtagcat atgcacaaca aaactataat
      781 tatcaaggaa ctggaacgat tcatatccat aaagtaacaa atactaatgg ggatgggaac
      841 cctgtctcag atattgataa caaagggaaa gaaatagctt taccaaatgg tgtaaaacct
      901 cttgaaggcg ttgagttctc ttatgtgaaa cttactaaag atgaagctaa atccttggga
      961 tggacggata gtgatactac aaaaggtcaa catattgatg atgctaaagc acgattatta
     1021 ctaaacaaaa aatacagtga agtaagtaga atgaagactg attcacaagg gaatatagac
     1081 atacctatac aagtaaatac agacgattta acagataact tgtatttgat tgttgaaaca
     1141 ggaacagtca aaggtgtagt tgaagaagcg tatcctatga ttgttaacgt acctgaacta
     1201 aacggatcag atgatcaata ctttaattat gatgtacatg tctatcctaa aaactatttc
     1261 caattaaact cagcagagct tgataaaaaa gacgaagatg gaaaggctct tgccggaact
     1321 gtttttgaat tgcgaaaaga tgatggatct gatcaggttg ttggaactta taaaacagac
     1381 agcacaggta aattaactgt ggatggtttg gaattaggtg actattattt cctagaaacc
     1441 gatttgggaa cagatgctaa tagaacaacc tatttactag atggtaaaaa attcaaattt
     1501 agcgttaaac gtggggagac tgctaaagta gttcttgatg ttaccaacta caaacgacct
     1561 gtaattacta aggtaattga taaagaaacc gttgatactg gcgaagatgt tcaatataca
     1621 ttgaaaccat caattcctgg taatattggt aattacgaaa aatatgttgt caaagatgtt
     1681 ctaggtgcag agttaactta tgattcatca gaagtgatcg aaggaatgac tttagatact
     1741 gattacagta tcgaacgtga cggacaaacg acaaactatg tctttactga atcaggtatg
     1801 gataagcttc agaaacaatt tgatgcaggg gatcgcttct ttacaatttt gatgaacgct
     1861 catactaata gtaaagctaa aatggataca ccacttaaga atgatgtttc aacgatctac
     1921 aacaatggat atgaacccga agacgaaaca ccagttgatc acgcttatac aatcattggt
     1981 gatcaccaat tcattaaaac agatggagat acaagcaaag cattgagtgg agcaaaattt
     2041 gtagtgaaga acaaaattga tccgaataaa gttacttacc taaaacaaga tccaacaacg
     2101 aaagctgtta cttggattgc agataaatct aaagctactg aattcgtttc cggatcagac
     2161 ggtaagttcc aaattgaagg tttgcaatat acctatgatg tagattttga taaacaaggt
     2221 aatccaacaa tcacagataa aaaaatcaat gattatgctc ttgaagaagt tgttgctcct
     2281 tcaggatata caccaactag aaacgatgtt ccatttgtgg tatcgaatga tgatgcttct
     2341 agcgaagtaa cagacatcaa gaaccttaaa acacctgaaa cagttcgtac aggtggaatt
     2401 ggaacagtag tatttctagt ggttgcaggt gtggcaggaa cagggctata tgttgtctac
     2461 aaacgagaac aacacaaagc ttaatgaaat aaaattccct agagtgtttt tggctctagg
     2521 gtttcttttt aaaaagtgtg tgaaaagtag gtatgagcat gaaaaagaag aatatcaagt
     2581 tgatcatctt aaaaattggg atgttgctat gtttaggagt aggtctatat cctgtaatta
     2641 ctcaggctta tacctactac caaactcaac aaacactaaa ctcctataag gaaaaatatg
     2701 atgaagcttc tgaaaaggag aatgaagaag tcttcaaaag atattcagaa gttaatagga
     2761 aacgaaaaga cacagaacaa acctcagtca cgattcctga agataataac aatattcctt
     2821 caaatggaaa tcttcagaag gataaattag gtgaattagt gggtgaaatt cttataccaa
     2881 agattaatgt ggatttgcca atttatgatg gcgtaaacga ttttcaacta tcagcaggag
     2941 caggaatatt aaccggaact gattatccaa ctggtgggat tggaaaccat tctgttatat
     3001 cagctcattc aggcattcct aataaaaaat attttacaga tttagagttg ctcaaacttg
     3061 gtgataaatt ttttattgat atttcaggga aaaaacttgc ttatcaagta gatcggataa
     3121 aaacaattct accaaccgaa tttgaggatt tgcttcctgt ggcaggaaaa gattatgtaa
     3181 cacttatgac atgtacacct gttggagata attctcatag gttactcgtt agaggggtta
     3241 gcatacctta ccaaaaaagt attgttgaga aagaaaagaa gaaagaagat ttaaaggtaa
     3301 aggcgcagat gaactggtat cctatcgtag ttgtaggggt tttattaata atagctgtga
     3361 tagtcattat taaaaaaata gtagcaaaga gacgaggaat gaaaaatgaa gaataaaata
     3421 ctactttttt tgactcttat ttgcactttg gttttaacaa tttatcctgc aaatcagctc
     3481 tatcatcaat tcaaaacaaa agagaatgtg gcggaaatcc aagagaaaat tgagaaaaaa
     3541 caaactcctt caaaagagga agtggcaaaa aaagtcgaac aaataaaatc gaacagcggt
     3601 aaccaaggat tagacaatac tcagaaaaca gataaaggag tagacaatac tcaggaaaca
     3661 gacggtggat catttgatat tcgtgaagct gaaggaaagg tcaatccata cacaggagat
     3721 gttttttcaa aaaatcaaac gaaagatttt gaaccatcta cctattcagg agcggttggt
     3781 tatgtctata ttccaaaaat aaatgtgcta gagcttttgt ttctaggcgc ttcacaggag
     3841 catttatata gaggtgtggc acaaatgagt gctactagtc tacctttatc aggtaaaggt
     3901 catcaaacag taatagcagg tcatcgtggc ggttatacgt caccacaatt tctttatgtt
     3961 gatcaattag tagccggaga ccacatttat atagattatc ttggtgaaaa agcagtttat
     4021 acggttttcc gaacagacgt tattagaaat tacggtcaga attcaacact gactgatatt
     4081 cctgatttag atatgttaac actttatact tgtcatccat atcctcagaa ctacgatcgt
     4141 ttattagtta gaagtctaag agatgaaaaa gcaaaataca tcacgccaca agtaacagtt
     4201 ggcaataccg catacgctga tgccattgat agtgaatccg tttcagataa gccagaaact
     4261 gttacatctt ctaatgattt atttgaggat gttgatcgtg ccaacaagga attagatgga
     4321 aataatgatc ttaatattcc ggaacaaggt agcgtagaga tgcaagacag aacgtttgct
     4381 gaaaaggcaa gttcaatatt tacaggaatt accaataaac atcagaaaat gcttttagtc
     4441 atttggactg tattaggagc attagcaagt ctaaaggttc ttattctttt aatcaaagag
     4501 tttagaaaag cataatgaag attataaaaa ttgatagagt acagtaaaaa gggattgata
     4561 ctattattga tctctttttt attttgaata gagctagatt cacttttaaa taaaatcaaa
     4621 actgatctta attccaaaga aaaagtcaag aaaaaaatac aacaaatttt tttatcagaa
     4681 tatttatcag aatattatta gaaaagataa actcaaaagc ttaatttaat agggttattg
     4741 gttgtattta gaaataatgt atggaaaagc tataaagcaa tagataagat gtagaaaatc
     4801 ttatttgtga atgaaaaaca agtcatcgta aaatattaat aggaggtgta gtaatggtgg
     4861 aaatatcaaa aacaacttta gactcattag ctaaactatt tagtagcagg tttgatggtg
     4921 cagaatttgt tgaatatcta agccaaaacg aaagtggaaa aactctgatc gaaacactga
     4981 aaaaagacta tttgaatgaa aatgactttc cgacatggac gaatattgaa gatgctacat
     5041 gggatgtagt gtgtacgata tattctcctt gggtaacgat tgctgatttt gttttagata
     5101 aaattggagg ggatacatac cctgatgcca aatctcttgc tacaagtgac tttaaaacgt
     5161 tctatggtta tctaatgggt ctaataggca atggaataga tttgaaagtt acgccaaaac
     5221 aaacacctga agaaattgaa aaagaaaaag gagaggttca aatggcttat gcagatgaca
     5281 gagaaaaagt aaaattattg gaaggtaaat tatctgaaga agaacttgga aaaatggtgc
     5341 aagaaatcaa tgaagacagc aactattttc ctgaattgaa ggtatatcaa aacgacgaag
     5401 atttctttaa tgaaatgttt agtaatagtc catgggaagc agtgagtaaa acaaatttag
     5461 gagaatggaa ctttcaagat gatctagttg catttagcaa tgatgggtat ttacgatcat
     5521 attcagaaag tgagtataga gctgaattaa aaacgtatga aacggatatt ctgatgagct
     5581 atttaaatct attagagagt ggtcagggag agttaagaga taaagttgaa gaactattaa
     5641 aagaagagct atagggatta tttcaacata aacacaagca gaaaactacc aaacaaaacg
     5701 taccatgcaa aaagccggtt aaaccggctt tttgctctat tatgagagga gatcaaacca
     5761 tgagtgcaac aaccgatatg gctttagaag cagtaataaa agataataca ggaaatgtcg
     5821 aaaatgtaga agactttagc gatattgcaa tcaatctttc agcatggatt tcagctgtag
     5881 gagaagaaaa tatcgaattt gagatcaaga atatggatga cttaaaagca aaatttcctt
     5941 ttatcgcatc gaaactaagt caatttgccg aggagaaatc gtggaatgaa ttagttagtt
     6001 tttccgaaaa aatttatgat attacgcaag aagatgagtg tgtttcatgg gaaatgaata
     6061 atcaatacaa gaaaattgat gctttcgtca aatcacgtat agaaggaaca gcttttgagt
     6121 ttgaaggtat agataccgca gataattggt taaataaaga atcctttatt aaaagtatag
     6181 cggatgatct tgaaaaacaa tggtctaagg aagactatgc agagtaccta aaaacagaaa
     6241 gagatacaga ggtgaaagaa aaactcactg aggtatgttt ggatgaacat attgatccca
     6301 atatcgtaaa acaagaagac tatgaagtgg ttattgataa agttcctact gattttgagg
     6361 aagttgtaaa gtatatagtt gaagaagcat tatcgcaata ctcttcagat gaggaagtgg
     6421 acgaagaaac tgttcgtacc agagtttggc aactggtttt tgatgacaaa gattataaat
     6481 atttgagtga tcaaaactcg gttaattgga gtgtacgatt attaattgat caaaatgata
     6541 ttgaataaaa tagagctgaa aagctctttt ttttttgata aaaaaactag ctattttttt
     6601 aaaaataatt tggttcaaaa tttatttttt taacttgata attttttgcc atcctttttt
     6661 aaattggcta tatatcaagg tttatagtgg ggataaataa aaatacattt gacaaaattt
     6721 acaatatagt ttattatata tttatcttat tatgataaca caataaaagg aatgatggta
     6781 tgaaaaagaa gaagtttagt cgatcgttgt taggaatttt agttttagtt gcggttggtc
     6841 ttagtggcaa gggttacttt cagaataaca gcactcagga acatgatttt cagatcaaaa
     6901 gtagtgttca ggtaggtaat aaagcagaca gtgaatcaaa agaattttta gggatacgtt
     6961 atgatccaaa caaatatcca aataatatcg taaacattaa tgatgggaaa tatacactga
     7021 caaaagaaga ccatgaagca ttatcagaaa aaggcagtca aaccttttgg gtaaattatg
     7081 cagatttaga tcaacttgga agagcaggag atgttgaagc gttgatcacc tatgatgctg
     7141 taaattctca ctcatctaaa aatgtggaac gtccatcttt caaaagttct gtccatattg
     7201 caggagaata caaggatgga gtatttgatc aagagagaga tacttgggtc ggcaaagatt
     7261 caaacaatga aatcactcag ctagaaggat ataaaggtta tctctataac aaaagtcatt
     7321 cattagcatg gtcgctagga ggagatatgg aggtacacaa tttaacactt ggaacgagag
     7381 cgcaaaatgt tgggaaaaat gatcagaatg gagggatggc ttactcagaa acgattgtac
     7441 gtgatgcaat ctataaaaat aagaatgaaa aagtattgta taaagttgat ccaatttata
     7501 aagggaatga attggttcct cgtggttctc atgtcgaagc tttctcagtt aacgataatg
     7561 gaaaaacggt aaaccttaat gtttatgtat ttaacaatca aaaaggaatc aacatcaact
     7621 atcaaaatgg tacttgggta agagattaga aattgagaag gtgaagaatt ggaaataata
     7681 agcatcaatg gattagataa atcagttgca aatcgaataa aaaaaggaaa tggctactat
     7741 cttctgaaga agggtaaata ttcagttgct agagagttag aaacaatgag tatcgtgtat
     7801 gtagctaaga atcattttga cgtagaacag gctatttttg atccagattc tgaaataggg
     7861 aaatgtgttg aattatcaga gaaaactgtt agtacaaaag gggcgatgat cattgagaga
     7921 gaaatgttag acgaaatcag agtaatcatt cctaaagata cgcagatgtg tgaggacagc
     7981 ataagtgacg atgaaccaaa atcagaaaga aaataaggag atattcagat gagcgaaaaa
     8041 tccattgaat taaaggaata taagcgaagc gatggagaaa aatattatcc tgtagccagt
     8101 ttttctaaat cagtatatct ggttggacgt gcattggtag ttggtgcaat tggcatatat
     8161 attttgcatt gggtaacaaa ggttgtacaa ctgcttgtag ataccttagt aagtattctt
     8221 gtctcgttaa atgatgttaa cggaacagat gatttaggat taaaagaaat gtttaaagag
     8281 ttactcaatg cgttttttcc caattattta agttattcat ataatgtaca aagctttttt
     8341 gcaaagctac acttggtaga catctctatt ttagtattag aagcaggaac ggtattcttt
     8401 gttggatatt tggttacttt gatgttcagg catcactatg gagaacaaag accatttttt
     8461 gatgatatgg aaagtagaag gttgaaaaga gaagctctag aagctatgga tgtgaagtat
     8521 gatccaaaca agattatagg agatgaaacg aacaacaaaa aaagaaaacg aaacaaaacg
     8581 caattagaat ccgatgtgct atcaacgctt aaaaatatca aaatttctgt tcatacaaga
     8641 cgtgatctaa gtgtcaatga agatattcga gaatatatcg taaaaatccc gcaaccaaaa
     8701 gggaagaaag ttagagaaac agttttacga caaattcaag atttagggtc gatcctgaca
     8761 agagcaacaa gcggacgagc caaatttggt gaaaaagaag atgaagtttc aggcgattat
     8821 ttcattttcc gagcatcagt aaatattgaa agagaacgta aggaaagagt aagtaccaaa
     8881 aacaaagaaa cggaaagagc aacatcttca atcgcagttt caaattcaca attttctttt
     8941 ccaatcaatc tctttgccga taattccaaa aatatccaaa accaaaccga aaaagctaga
     9001 aaatatgccg aaactcaaaa gcaatcttta gaactttatc tatcttctgc tgacgtacct
     9061 gcaagtctaa cagatatgca cgttgggaat acatctattg aatatttgtt tactttatct
     9121 cgtaccagaa aaattcaact aagctcaatg aaagaagaaa ttgaaactta cttgcatata
     9181 tcaggtgtaa tcgtgcggat caacgcaggg cgtattcaaa ttactttacc attaggagaa
     9241 aatgatcgta ttccaattga tgtaccatca atgatcaata gcgtgtttta aaagacatca
     9301 caaatagaaa cagaaaatac aaactaccag ttcacaaaca gaaaagtacc gagaaaagtg
     9361 aggaatagaa atggctatga aattattaga gaaagtcaaa aagagagccg aaaaagctga
     9421 atctattttg ctagataaag aacctaatat aaagcttaat ggaaaattca gtgtcaatat
     9481 gttgaacaca aaggctaaag acccgacaga tgctattttt ggaattaagc ctgatggcac
     9541 aattgctcat ttttgcatca gcgaaatgcc acacatgttg gttgcaggaa cgacaggatc
     9601 agggaaatca gtattactga atgaaattct tgttacagca atgtgtcact caacgcctga
     9661 tgaattaaaa ataggaattg tagacccaaa aagggttgaa tttggaagat acaaaaaatt
     9721 gccgttcatg ttggctgatc caattacaga tatggatgaa gcttatgatt tttttgaata
     9781 tcttgtgatc ttgatgcatg aacgttacaa actgatggaa aaagcagggt tacaaaacat
     9841 cacgctatat aacgaatatg ccgaaaaaaa tggattagat agatttccct atgtgatctt
     9901 attagttgat gaatattcac agttggttgg aacacataaa gaagtagaag gtttgattgt
     9961 tcaattagga caaatggcaa gggcagcagg tattcatgtt atcttagcta cacaatctcc
    10021 tcgatcaaca gttgtcactg gtattatcaa agccaatttt ccgtcacgtg tttgccttat
    10081 ggtagcaagt gatttagaaa gtaggatcgt attagatgaa ggcggtggag aaagcctaag
    10141 tccaaaggga gatatgatta ttaagctcgt taatggaagt atggttagag cgcagggagc
    10201 ttacatcagt aacaaagaaa ttgaaacaat tttcaatcac cttagaaata ctatgccaga
    10261 accagaatat gttgattacc gagcaatcgt agctgaatct cgtggtgagg gtggagaaga
    10321 cgaggatttt ggtggaattg gtaaagctcc tgtaagaaaa tctgctaaaa gaaatttgaa
    10381 cacaccttta gcaaattcat catctaaaga gccatcaatt gaaaagaaga gtacgtttgc
    10441 tctaaaactt gaaaaaaaca aagaagaaaa agaaaaacaa ggagctaaaa cactagaaaa
    10501 acttcaagcc gaaaaattag aaaaagtttt aaaagcatta gataaagctg attctgaacc
    10561 gcaaacctca aacaacacga ataatgaaca gccgatttca tctaaaacta aaccgaaaac
    10621 tactgatccg aaagtttcag taaacgtgac catcacaacc gataagtctg agaagaaaca
    10681 agtaaacgga acgacttcac ctatcatgca aaaaatgttg aaagccaaaa aacaggtgca
    10741 taaatgatca ttgtcagttc ttttttgagg tggcttacgc catataaata tacagatgaa
    10801 gtagttgtga aacgaacgaa gaaaagagat aaaaaagcgg tactaactcc taaacagatg
    10861 cttgatcatg gcattttagg ggtgaaggtt ttagaagtga ataaaccgtt tgaatcaaat
    10921 agtgcaagag aaaagttgct tggagctgaa gccgatgtcg catattttta ccatgacaaa
    10981 agcaagcatc aaatagctat taaattcaat ccaaaatcgg catggcggta tcaagcaaga
    11041 gatgacttga aacgtaggaa acttagacca atgctctatc cagaaggaaa agaagcattt
    11101 gatagaaccc acatcgttcc cattggtttt catggtacag aatcagataa ccgcctatta
    11161 gtaggttgga acagcaatca aaatagagtt cagatcaata aatttgagaa gaaagtaagg
    11221 aaattcaacg atcgaaaaac gatcctatgg tttgcagata tttgtaaaca gccggatgga
    11281 acagcaaagt gggaagcggt gatatttgac gaaaatggta aaaaagtaat gtctgaaaca
    11341 tggcatgata aagctaaatt tgtttggcag tagcagtaga ggtgaaaaag attgctagaa
    11401 ctttttgaag caaatagaac aggctttaat caaaaattag aagtgggaga aactatagaa
    11461 tataaagata aaaactatat tattatcgga atctataaca cgagaattaa cagaataggt
    11521 gatccacgta taaccagtga cttggtttgt caatctgtta actattcacc tgatctgaca
    11581 agcaggtata agaaatacgt gttattagaa tatagacaca agattactga tgaaattggt
    11641 gtaaacaaca cacgaaacat atttaagatc ggaacagtga ttccttatag taacaacgaa
    11701 gaaaaaatat tttatcagat ttacggaata gaaaagtttg aatatgaaca tgtcgatctg
    11761 ctagtgacgt accaaatgcg tttaatagaa ccttggtcac aggcagaaat tgataaagca
    11821 gtaaaattga accgattatc aaaatttaac gtattaggaa atgcctttta aaaatcgcaa
    11881 tacacaaaca gaaaaatacc aaaaaattag caatgaacgt aggtaagaac atgacaataa
    11941 ataataataa ccctaaaaaa tataatgctc ttgtccaatg ctatatcgaa aacaaaatta
    12001 caatgcaaga agtcgctgat gaattaggca aatcaaagag cctagtatca cacgatttta
    12061 agcacaatgt ttttccgaac ccgattgagc tgaggaaaaa atttcaaaag agagtttatc
    12121 aagaaatagg taagtggatt gatgaaggga atgcaattga ccctttaatt gaaaaatatc
    12181 cttttttaaa aagcaaaaat aaaattcaaa ggttaattcg tcaaggcgat attcaagcta
    12241 aagaacctgt tttgacaacc aatgatttgg ctaaaataaa tcttcataaa caaattattg
    12301 aagactttga aaaaaatctt tcaacaactg aaattttaaa aaaatacaat attccgagag
    12361 gaaagctaac atcgactctt tttagagaat atggagaatc ggcagttact aaagcaaagg
    12421 aagtagcgaa tgagagaaaa aaacaaaaat agtagtcgat acctagtagc gactgtagtt
    12481 tttgtttgtg tattgatttt cctgtttgat tttttgcaga ttggaaaaag cataattgga
    12541 aatacattga gtgcaaagga aatggttgct ttaggtggtt tgtatgaagg acacttgttc
    12601 ccgaatatat tcatttctat cgtttctcat attaacttgt accatttaat ttctaattta
    12661 ttgttcttag tagttcttga agaagcactg aatgatcagt atgagtggtt aacattattg
    12721 atcgttttaa tcatatcagg atttctagga agcttagttc cttttctgct acacagcaac
    12781 atcgtaagta tggggcttag tggaggtatt tttggatgga ttgctttatt tgcgattgca
    12841 tacatccatt ttgatagcaa gaaacattta ttactaagtg tttttgtatt attgataatc
    12901 ggcattttac tggtatctac ttttgaatca ggcgagacaa acgtcttgtc acatgtaatc
    12961 ggatttgtct gtgggatgat cttgggattt attctaaaga acgtttcatt gatcaaacaa
    13021 aagaattaaa agggagtgct aaaaatgcca aaaaaaaaag aaaagaaaat tccagtgttt
    13081 ttgtctgtct cagaaatgtt atcacttcaa atactgttaa gaaaaaagat tgaaacaata
    13141 gacgaagaag ttagaaataa aagggctatg tacgaacaac gtgaagcgct aagagaacga
    13201 atccactatt ttgatgatat acacactaaa atctatgaag cacgtacaaa tcacgaagac
    13261 gaaagcaaat taggattagc tcaacaacga atatttgaat ggctgaatac gaatactgag
    13321 caagaaccgt taagagcaat tagtaatctt tttgaatttg gaacgaagca ttggtttaac
    13381 gctgtagaaa aggatgctga cttacaagaa gcgttcgata cactaagcga ggatagcaaa
    13441 tccatgcgag acttattgat cacttatcta aatgagcatg aatttgaaca gacgaaagtt
    13501 aaaaagaaaa ataaaaaata gaaagttgtg atgtatcatg ctgtttacct tattaggtta
    13561 tttatgttta gttcttgctt tttttggagc aatagctata tttgtcggtc agtttcaagc
    13621 aaataatccg ataatgcgta acataggcat tggaattgtt atcatatcct tgattgtatt
    13681 tggtgccaca aagatttttg ggagtaatga tcagcagttt ggtatttctg gagttgataa
    13741 tagttatgct ccagattcat tgggaagagt agtgatagat ggaacgtttg agaatgtaac
    13801 gagctacaaa aatgtttcat tgacagtgaa agaagatgac aagaaagcat cagatgacgt
    13861 tctcgctacg atcaagccgt ctcaatcaca gtttgaagtg ttttatacag tcaaagagaa
    13921 taaccctgta cgcaagctaa cattagtttt taacgttgac ggagaagaat actataaaga
    13981 tgtaaaaatc aataaagcat caacgaaaaa gattgtcgaa tcagcgaaaa aagaagtagc
    14041 tacagaagaa agtacaactg aatcaagttc tactgtcgaa gttgaactac cagataaaat
    14101 tgattttgat cctgaaaaag tcaaatcgtt tgaatctgtt tctggttcta tggcagatcg
    14161 gtttaacact gaagttttca aagctatcaa aactgttaaa ttggatactg aaaagcaaag
    14221 gattaatgtt accttattca atgcagttga atctatggat caggataaaa aagttacggt
    14281 tgcaagagaa ctagggaaaa gagtaattgg gttgtatcga ggtacggcaa aaccagattc
    14341 agacggttca gatttgccag ttcacgtagt ttatgaagac ggtaaagttt ttggagactc
    14401 tacagcaaat gaagcactaa cgattaaatt agctaaatag ccggaaaatt ataataaaca
    14461 agtggtgtca caccaccatc gcataggtgt gacaccttgg agatgattac tgatggtagt
    14521 aaataaagaa aaagtaagaa agtatgttac tttaaaaata gcttctgttg aatatttaga
    14581 aaagcgagct aaagaagaag aaaaaacaag aggtaagaag gtaacagttt cagagttgtt
    14641 agaagaatta attgagagtg accaaacaat aagacgtgca tttaccaata ggtctgttaa
    14701 aagttagggt gatactatct ttaatttcaa ccaaatttct aatatatgat gaatagtttc
    14761 cttgtacaaa atatctaatt aagagataaa agttaggagg attcattcaa aatgattatc
    14821 caaaagtttg actggaaaat gaaattagga attagtcttg taatattggg atttattatt
    14881 tttttgggga taaaagcaca aaaaaatcaa tctacttttg aaactatgtc ttctcaaatt
    14941 attgaggaat gttccaaaat ttattattgt gagaacaaaa ctgaagaatc taatggcttt
    15001 tattatatcg atcaaccaca tgatgttgtt ataaaaatgg agtcacatga agtaaacgaa
    15061 tggattcaaa gaagtactaa tgtatcaaaa aagttagaaa gatattctca aacgttttta
    15121 agtaaagtca ttttttcaaa ttgcaaaaat aaagaaacgt ctcaaagagt agctagttct
    15181 ttagaaaatt taaaattttc tgtttcaaaa aaagagaaat ttgtagattt acgaaaataa
    15241 actatcgaaa gtagtatgat tatgatctta aaatatcatc ttaagagaag aattacgatt
    15301 gtttatagga tttacaaaat gaataagttt gaagagtgat ttggtcgcag actagatcac
    15361 tctttttgta taaaaaaact ttacacttat ttttgagtgt gttaagattt tttatataga
    15421 gtgacggttt cttgtcagag tgaatgataa ccatcaatat tggaggaata ataagtggaa
    15481 aacaataatg atattagata ttttagcatt ggggaaagag ttacaacagt aagtgaatta
    15541 tcaaaggatt atagagataa agaaggaacg attatttctc ttaaaaagaa tcgaagaggt
    15601 gtttggcaat atgttgttga tttaggggat ggcgttacag ttgagtgtaa agatagtcaa
    15661 ctgatcagaa atgatggcag tcattccatt gaaaaagcaa ttcttgtatt cccaaaagta
    15721 tcaagcaaga ttatgaatga ctggttgctt ggaaaatttg gtgagtacaa taaaaaagaa
    15781 atcgcagaag tgatcggcaa atcttatcca acagtattag ggtatctaaa aaagataacg
    15841 aacaaagaag cgaaagttga ggatgtttta acagtaagag aacttcagtc attaatcgaa
    15901 tatcggaata aacaaataac aaatcctgag taccttaaaa aagaagatgg tctattgaaa
    15961 caattggaac aggtaaataa aatccggaaa atgcaaaagg tagctccttt aagcctagat
    16021 aactttaaaa aaatctattt gaaagagatc gaaaaagact aatgtggagt gtcactttac
    16081 cgtttcaacc tgtatctgca ccaagaccaa gtgcaagaag gaatgttgct gatgcaacta
    16141 atgacgtagc agagaaaagg atttcaacct attatgataa aaagtatctc gattatttga
    16201 aaaaaataaa aagctttatc gaagatcaag gtttgttaga tgatgaattt tactcgatcg
    16261 taaatgatcc gatgggtgca attatggaag tggactttta ctatcagata ccaaaaagct
    16321 ataaaaaagt ctataacatt atgaagacga cagcgccaga tttagataat ttagtaaaaa
    16381 gtgctatgga tggtattttc aacatctcag ctatacgaga tagccatatc acagggttaa
    16441 ttgcttttaa atacaatgtg gcaaacgaag gaaaaacagt tgtgaggatc aagcgatatt
    16501 cagaatttga gtgggatact tctgaaggat tgaacggaga tatttgggaa gccacatttg
    16561 atttcaaacc agtagcaacg ccaagaccaa agtctaaatt tagaggaaat gggattatca
    16621 cttactatcc aaaagagtat aacgactatt tggaaaatat caagaatacg cttaaagaga
    16681 aagatttggt aaatgatcaa ctgaaaaagg taatgaatgc tcctatgggg gttatagctc
    16741 agatagatta tttttaccaa gtgagaaaag ataagaaaaa gcttgattca ctgatgcgta
    16801 cttcacagcc ggatattgat aacttagtca aaggaacatt ggacggaatt tttaacaagg
    16861 aaattggcat caaagatagc agggtggtag gtttaatagc ttttaaatac aatacttttg
    16921 aaaagtcgaa aacaaacgtg cgactacagg agatgggctg atgaataata acgaaaaaat
    16981 tcttcatgta cttgatagct ttgaaacaat tcaagaggaa ctaaagaaat atcgagatgt
    17041 tttggaacaa agatatgatt ttgtcaatca gcaaaagagt aatcacatgg atttcattct
    17101 caatatgaat gacttgaaga agaaattagt tgaacgaaaa gaacaagaaa agttgatcaa
    17161 agcttacttt gaactcggtg aaaaagaagt taaaaaggca atggaactga atgaagatcg
    17221 cagggtttta gatcagctgt tagaacaatt attggtgatg tttcaaaaag gacgaataga
    17281 tgaggattta attgaagaag ggttgagaaa gtatcctgca aactcaggta tcggaatagt
    17341 gctgaaagcg attgatgaag aagaaattga agatttcata ccggcagagg attttgaatc
    17401 gggaatggaa tatataaaat attacagcca agggatcact gcttttagag agtttgatcc
    17461 tgaagatgtt attcatgact tgaacaacct aaaagaatgg tgtgaaggtt atgaagttga
    17521 tgattccggt ttggactact tgatcagtat tatggaaatc gaagaagaga tgccggataa
    17581 gcctgatcca acagatattt tagaattaat ccatgatgct agaaatccaa ttgcatacat
    17641 ttctagagga tatactgtat tggaatacta taagccttat atatcagcga tgaaccattt
    17701 aagacgtgta ttaagggaaa aacgtgaata ccgctcagtg ttaaatgcat cgaatagatt
    17761 agaaaaagct gtttcagagc tagatgctta ttatcgtgaa cattacctgc aagcaggtgg
    17821 tatgccaaga aatacgaaag caaatatcag taggtatata aaagaagctg agtaggcgga
    17881 gttagcccat aaaatggcaa aaggtaacct gaaaggggcg gtttgaatga aaagggatgt
    17941 ttttgaattg tacgtattag aacgtctaga agacggcatg atggcacaag agataattga
    18001 tgaaattgat gatatgatca gcagatttgg tcaattggaa ttgaatgatg gaaacaaaaa
    18061 ttcggtagtg ctaaaacaat tttggttaaa taatttttcg tataaggatt ttatcaaaga
    18121 aaaggcaaat agattgtact gactaaattc acgaggatca gtgatgagag cttattgcta
    18181 agagcagtta ggctcttttt tatttgtgta aaattatttt attttacaat acacatttat
    18241 acacaaaaaa tgttgacgga tagtacacaa atgtggtagg atatatttgt aagcaagtta
    18301 gattaacaag ttaaaggaag gaagagcagt tatgaaaaaa gaagaactaa tagatatgtt
    18361 tcaaattgtt gaacgagcaa ataatatggg aattatgttt tttgatcgta tttctttaaa
    18421 gatggattta agcgttgccc accaagaatt taatctacga ttaaaagctt tattgatttc
    18481 tgatgatgtt aatttcgctc atgatgttgt aggtatacag aaccatatag atagagaaaa
    18541 caaacgtatg ggtgatggat tcttaccaag atattcaagt ttatgatttg gcgaaagatt
    18601 caagagtatg tgtcattatt gataggtact cttgaattgg aaaggaggaa tgaatttaag
    18661 acaacaacga ttatgatctt aaaacaatcg tttatgtaat tggcaatctt acgaataatt
    18721 ttcatgcaat aatgacaata aaaaatgaaa aggggtaaaa tcaaatgaaa aaatttttag
    18781 taggaattct attcacaatg gggctagtgc tattatcacc ttcagtttca gcagaaagct
    18841 ctatttccat gtatcgtatg tataatccca atagcggaga gcatctatac actagaagcg
    18901 ttggagaaag agacaatctg aaacgttatg gatggcatta cgaaggtgtc gcgtggcaag
    18961 ctccaacaag tggttcccca gtttatcgct tgtacaacag atataacggt gaacattttt
    19021 acaccgttga tgccggagaa agagataaga tttcgcacac aggatggacg tacgaaggtg
    19081 ttgcgttcta ttcctacaac ggagcaaata gagttcctgt gacacgcttg tacaacaaat
    19141 acgtgaattg gcaccattac acgttagatc aaaatgaaaa gaacgtaatt agtaaacaag
    19201 gttggaagat tgaaggtacg ggttggtatg caatgccaaa cagcgcacca aatcctacac
    19261 ctgcacctgc tccaagtaat gaaaaagtgt taaatgttcc tgtcatttgg caaggtaata
    19321 cgatgttgtg tgaaggtgct tccctgttag agggattgca ctacaaagga gtagcgacaa
    19381 atcaaaactt aaccagtttt gtaaactata tgccaagagc taatgacaat aatccatatc
    19441 atggctattc tggcgaatgg cgctacaacg taaatggtac gtatcaaggc atgatgcctg
    19501 atccagtagt taaatgggca aaaaatgttg gtggtaatgc cgttaatatc actggttcag
    19561 gaacgaatgg gatcaagaat gaaattcgca atggtaatcc ggttgtaact tggattactt
    19621 ataactatgc ttcacctcaa ttcaaacaaa tgccttgggg gcgtgcagtc tggaatggac
    19681 atgtggttct cgtggatggc ttcaaaaatg gtgcatacca tgttgttgac cctgtatttg
    19741 gcgcaaaatg gatcaatgca agcacgtatg agagagcatt taatgtgact ggaatggcgg
    19801 ttgctgtacg ttaattgata gaattaataa ataaatgaaa taagagctta attactgaaa
    19861 tggtaaatgg gctcttattt tttagaaaaa aactgttttt ttgtaatacg aatctataac
    19921 aaaaaaacgt tgaatgtagt acacaaaagt ggtatattaa aaccgtagag attatgcgaa
    19981 gaggtgattt tgatgaacca aagaagaaga aaaaggctag aagaaataag cgagcgacta
    20041 tccgagttaa aggatgagtt agaagtaatt caagaagaag aggaagagta caaagaaaat
    20101 atacctgaaa atctacaggg atcagaaaga tatgaaaaag cagaagaatc cgttgatcaa
    20161 cttgaagaag ctgttgatgg tttatccgac atattagaat tgattgaaga tatagctagc
    20221 tagagatgat taatgaagat ttcaggagtg agcaaaaaat gattgatatt tcactgatag
    20281 agaagctact tttttcaaat aagtacacag ccaaagagtt ggctacaaga tggcaaatat
    20341 ccgaatcagc tattaaagcc tatcgtcaaa ataagggaag cgtaggatgg cgtgattgga
    20401 aaggaatcag tattggaaaa gcagaagaca taatgaaacg tgaaggattt caagctgaca
    20461 atgattctaa aaaagttgaa tagtttaata gtcgccaaga aaagtaccag gacgagggga
    20521 aaggtagatt acaaaggaaa acacttttaa ataaacttcg caaaagcatc aggttatgag
    20581 tggctagggc tatacgaatg tgatgaaaac aaagggcttt aatgtcaaag aaaacgcaat
    20641 agggggggaa taaaattttt aaaaaagata tttatgtttg gtttaatgga aaaaaatata
    20701 tgactactaa caaaaggtgg tcagagctag tacaaaatac agttaaaaaa gtcatgccag
    20761 atggatatca tttcaactat atacaatgct cacaaacaac tgaaagtatt tatttttcaa
    20821 tattaaatga gaatagactc tattatttca gggtaagttc acaccatagg aaaaagagag
    20881 aagataatgt tacgacattt ttatcgggaa aatacaatgg cttcaaagag ttaagtgaag
    20941 caattggtga gtatctaaaa agtaatagat ttgtcagaat ttctgacaaa catatattga
    21001 tcttgttact ttatttgaat aatagcttca aattattttg gagagacgac actttctact
    21061 atattgagaa cggcatggat atttattctg gtatcaacgg catattaaaa cattatatta
    21121 aggcgttact tcgatatggg gttctttatc aagaaaattt aagtaaccaa attaagatta
    21181 gtaattctgg aaagtcatta ctggcaaaat ataaaaaatt gctttctcct atttacaaag
    21241 gaatagatat ggaagctgta aattctgaaa ctttatttaa tttagttgtt gatgaaaagt
    21301 ttgcgaatga attttttaaa agtgaaaaaa ttgaaagact aaataacctg tttcctgaaa
    21361 atgcaataaa agcttatgta gataataata aatcacgatt atacaactgg tttattccca
    21421 aacggttgcg aggaaaaatc aaaattggcg atcaagtgaa agtaattaat ctaaatcata
    21481 ctaagatcgt taccgtaacg gaattgtata tagctgaaaa agaagagatt acaaaaatga
    21541 aaccagttat taaaatacta gataacaaag agtgagtgga atatatggta gcaaaaaaga
    21601 atactctaaa agaagcttct gaaaaatatc aagatatgaa aagacgagga ttaataccaa
    21661 aggacaaaaa aggtatatgg agagaatttg ttaaggaagt cagtagtgat cgaagtggcg
    21721 gtgactttgt tggacgatat acagcaaaag gttatagcag gcgcttaagg atcactgttg
    21781 aatatggaac gaatgaatca gctacagagc tgaagcaaag gtacttagat acacgaagag
    21841 aagcttcaaa aaaatatgat gatttcaaga gcagaggtct aattcctaag aagaaaaagg
    21901 ggatttggcg tgagtttttc aaagaagaag atagcaatcg gaataacgga gacttcattg
    21961 gacgatacac agcaaaaggc tacactaaga atttagggat caaggttgaa tacggaactg
    22021 gtgaaagttc atgggaattg agaaatcgct atttaaatac attgttagag aaatataaag
    22081 cagaattatt ggagttgttc gatgttaatg attttatcga gatggctgaa aagtatggtc
    22141 atgaagaagg ttttatgatc aatgacatta tggttattga taattttacg gatttattag
    22201 cacttgcgaa aaaagcaagg gatttaagag aaacgcagat gtatctattc agaaataaaa
    22261 acggaagaaa ccggagaact gaatcgagtg agattgaaga ttactacgat gcctttacac
    22321 acgatctggg agcctttgta gacgagataa gtggtgtaat gaacaaatat agggtctaga
    22381 ataaaaaagc cggagatgag cagataaatg ggaaacaata gcatatacac acctgaaatg
    22441 cagaataaaa ttaaaagact tgtcggatta gggtggatga caagaggaaa attagattct
    22501 tttagtacga tcaaatatct tttgatggac atttaccgtc agaacaagca aacaggaata
    22561 cattttttgg gctatgataa gccggaagat gcacctttct ttgttccaag ttcagcctat
    22621 agagccagta cctccggtag catgtataac ttggatttgt tcgaggaagg agcaaagatg
    22681 atcgaatatg atttcaacga agcttattcg aggatcatga gaaattatcc tttgccgtca
    22741 aacacttatt tgaaagtaaa atataataca gatcaagcta ttttgagagc gataaataga
    22801 ccaaaagtaa gaccgtatga aaatttaaaa acgtatatgt tctttttggt taattttgaa
    22861 ggaacagcca aagaaggaac atatgctaat ccaaaatcac agtcgatgtt tgcggattat
    22921 ggcgaaaatc ccaaaacagt taatgagcct atttggttat cggagattga gctaacactt
    22981 atccttgatt attatgatat taaagagatg gaagtcctag atagccacac atttttgacg
    23041 aaaaaaggat tactcaaaga atattttgaa agaatcgagc cattaaaaac tgatcctgat
    23101 acgttgaagc tttataaaaa tcttagaacg aaagtatttg gtgctatcgg acaaaaatca
    23161 ttaagagaag gagaaacaag caccttcgat aaatacagta tgtataatcg agcgtattcc
    23221 tccatggtag caggcgtatt tagagatcgt atggttagat atgaacagaa atacgttcat
    23281 agcgcttatg ggctattaaa aattcgtact gatggattgt atttcagatc cgaagtacca
    23341 gagtttgaag agctgtataa acttggagta gttaagaaaa aagttcatac aatatccaaa
    23401 gaagaagtcg aaaaagcttt agaatttaaa aataaaggat ttgaaaataa gggctccttt
    23461 gcgaaatcta aagatgaagt taagggtgtt gtgatagacg atgatttcga gtttgatttt
    23521 gactaagtac tctttggaaa agatggtagg aggtaaatat gaagaagaaa attgtggaag
    23581 gaaatgtagg tgttattatt ctaaaagatt caactgacta tgctaaatca atggcactaa
    23641 aagacgaaaa agttgcacgc atggataaag atttagttga atttctgatg gatgatacag
    23701 aaagattaaa aggggatcag tttttgagtt taatgaaaga caaaggctta aacacatatg
    23761 gtttatttga ttataagaaa ttagctatag agtgggtaaa agaaggagaa atatttttta
    23821 ttgaagaatg ggaaggtact gagaacttag ttcttcatca gtatcttttg aaagcttaat
    23881 ctaagtaaaa cagttctgac tagttatgct tgtcagaatt tcaaaggatg ttttccataa
    23941 ccatcattat tcaaaaataa aggatttgaa aataagggtt cctttgggaa atctaaagat
    24001 gaagttaaga gttttgtgat agacgatgat ttcaagtttg attttgacta gatttctttt
    24061 tgcaacgttt aagagtaaac aaggtaagaa ttaagattgt gaagattgag agtaaagaaa
    24121 atagaggacg aatgtatgag aacacactat atttatttag aagataaaat tgtagatgaa
    24181 attccggcaa aaagtcgaga acaagctaga aaattattta ttgaaaaata caaagagatc
    24241 aatggagata gtgaagttcc gaaagtggtc gtaacagtta gaaaaaggca aacaaaaaag
    24301 agaagtacgt ttcatgtcag ccctgatata tataatctta tagagcgaca tagaagtgaa
    24361 gataatattt ctatctctca atatattgct acagttattg atgaattgat ttcaggaaag
    24421 tatgactatg cttttgattt tgaggatgaa aaggattaca aaagtgttgc tgtctttcaa
    24481 tctgattttg agaagctaaa aaactatgct ggcaggagaa atgttaattt tgcaagacta
    24541 ctagaagagt tctactccga agagttggga gtaggaaatg aatacaagag agaaacttat
    24601 gcgaaattgt ctcataagtt atcccaagcg cttaaagaag tgacagaagt taaagcggaa
    24661 ctagatgcta aacttgaaga agtgaaaaaa tatcaaacag agaggaagaa caacatgaag
    24721 aatcaagtgg aatttgacga atagaaagaa tttgaaaaag caagaaaacg ctataacaaa
    24781 attatgaatg aacttagcta tgaacactta acagttcatg aataattgag tgagggtaca
    24841 gaaaaatata cgatcgcaga tatggttaat gaagtggaaa ctcttttagc ttctttcttt
    24901 gaaaatggtc acgtaaacga tgaatggcga aaagaagaac ctaaaagggt taacgcttat
    24961 attgctcgat taaaaaggtt tattgataaa tataaggaga atagtgacaa attttctaaa
    25021 gataaataag gagagagtgt gattacattt gccagaaatc gaaggaatat tttcgccaaa
    25081 aataggtaaa aaacagttag atttcagcag ttttccgagc gtaaatagag attataatct
    25141 aggtgatgag gtacagatac taaatattga tttcaaagaa ctagacttcg ctgttgctgt
    25201 ttccaacaaa cccaaggcat tggaacgagt gaaaaaaata taccaagaga atgatcaaaa
    25261 tatcaaaaat atgatcaaaa agtatagacg agataattca aaagttgaag acgttttatt
    25321 caacggtacg gcaaaacatg atttgaatgt ttatatggca ttaggagtgg ctttgtactg
    25381 taattcaatt aatcagacag atattttggt taatatcgtg aggaatagct atcaaaaaat
    25441 attaaaaata agtagcgaaa caaaccctta ttatgcaatg aaatatcttg gggataattt
    25501 attaaatgat aaaaaattga tgcacaacac tgaagagctg ttatctaatt tggctgcgat
    25561 taactttcta ttcaaaacag gttctgaaga ttattttata gataacgtac taaaaggtgc
    25621 ttacggaaca gcattttatc cgctttacaa tatgaaacag cataaagccg aacaaaagga
    25681 taacaaactt gaaattaaaa agatgaaaga actattaggt ataggatttg taaaacccaa
    25741 ttcaaaagtc actattgctt cgttagcaac tactaacaat gatgggaaaa tagtaaatgg
    25801 gtttgacgat tcagatatag atatattgtc aaaatcatat ttttctttgt atggagattt
    25861 tgcagatttg atgggtattg atagttactt agtcaataat tttgagatca agggtagcga
    25921 gctgaatgat attgtgtatt tcgttttaat ctctctatat gcaattgatc agaataccga
    25981 gtaccgacaa attgactatt tgctagtttt ttatagtttg ttatatcagt tcgttttaac
    26041 tcggttgttt aaaaatgcta aggaagaatt gatcctgaat tctgattatg aaatagcaaa
    26101 acaaattgca agtattagaa aagaaaacaa atctgaagta atcaaattaa gaaaagaatt
    26161 aagtattgcg agtaaagaaa acggtcggtt agaatcttta gtcaaatcgg caaaaaaaga
    26221 agaggaactt ttgaagcaag agattaaaga attaaaagga atcagaaaag aacttgaaca
    26281 gctgaggttt gaaaataaag tattaaagca attgcaggag caagaacata gacaatcatt
    26341 aaatggtaaa actgaagagc cttcactgtc tgaaatgata gcttatctaa aacataaaaa
    26401 cattctttta gtgggaggac atgtaaattt tcaaaataaa ttaactgaac tattaccaaa
    26461 tattaaaatg tttgatgtaa gcgaaaataa tggaaagata cttaaaaatt tgaatacgtg
    26521 tgatgcgatt tttctatata cagattactt gaatcatggt ctatattatc gtgttatgag
    26581 tgaaatttct aataaagata agatatttta tttggataat catgtgaata tgaacttaac
    26641 aattaaacgt atttacgatc ttttatcact tatataaaga gagaagtgat aaataggaca
    26701 caaaatttgg aaagtaggta agtcactatg agagaactaa ctttatcttc taaggaatta
    26761 gaaaagaaaa ctgctttatt aaacgaaata tttagtgctg attgtataat ccaaagttta
    26821 aaagaaagtt cattttcaaa actaacagat caggaaaaaa tttcttatat gctaagttta
    26881 gataatctag gaactaaata tgatgaatca gcaagtagcg ataagctttt gattgaatta
    26941 gtatcaagtc ttagtagttt tggaaaaatg aaaagattta atgtgatatt agaaattttt
    27001 gcttggttaa tcccttcatt tatttggttt ttttatattt tcatagttga atctgagtta
    27061 atagccaagg tctctcttgg actgttaatt ccgatatatg cattttattt aataggttca
    27121 acaatttact ttaataactt gtatacacgt attcaaataa aaaagcagtt attacatgaa
    27181 ggattaagtg gtaatatgtt aaaaagaatt gcatgggata aagataataa aacagatatt
    27241 gagcttaaag aatataaata caattatttc tcgagatgta tagatagaaa gttactgttt
    27301 aaaaaagaag gattagaatt tagttttgat gaagatgaat ttgaaaaatt agtgatggac
    27361 tggcgtaaga atttactgga tacggtacaa ttctaaaagc aattgaggtc gaaattccga
    27421 tacttgaatc atgatctata ttatcgtgtt atgagtgaaa tttctaataa agataagata
    27481 tttcatttgg acagtcatgt gaatatgagc ttaacaatta aacgtattta tgatctttta
    27541 tatgtttata aacattaatg aggtgctaaa attgacaaca ataactatga caaaaaaaat
    27601 agaagtatat gttgatggat cacaagaaga aagggattcc ttttttgatt ttttttggaa
    27661 aaatagtaga gaatatgtaa aagcgttgaa tctggtatat aaacagctaa tgtttgaaga
    27721 gttgataaaa gatgcaatta aagaaatgga tcaaggttat gctgagagag aagaaagtat
    27781 tcaaaatcgg attgaaaagt tagcatatac tgatccaata gatcaaaaaa aacttgataa
    27841 agcaaaagct gaattatcta aattgagaaa tgaaaaaaat aaagaagcga gagaaatatt
    27901 aacgcaagcg atagggttga agagtcaaac gagaacacga gatgtagcaa aaaaattaga
    27961 ctttgacttt ggagattttg ttgattccgc aaatatgaaa gctagtaaag attttggcaa
    28021 tgattttatc ggaattgtga ccggacaaag aacgcctaga atatataaaa atagaaacaa
    28081 atatggatta ttacctttta gaggtcgaga tattaaaatc cataaagatg ataagtttta
    28141 tatttattta cctaaaggct ttaggtttaa agtgaattta ggtagcaagc caagaaaagc
    28201 aagagatgtt gcttcagttt tacaacacat tatggatggc gattacaaat taaatcaaag
    28261 taatcttatt tttgatggaa gaaaattatt ttttaatcta acaatttctt ttgaaaaaag
    28321 ctataatgaa atggaattca agaaaggtaa tgaattgatc gtaaactttg cagatttgtg
    28381 tagcggtgaa tgtttaatta atgataaatc tgtaatgtct tttggtgata gccaatatat
    28441 aagaaacttc aaaagtaaaa ttttagcaat gaaatcaaaa gaacaaagcc gaggagtctt
    28501 ttctaagggc ggtcatggtc gaaaaagaaa attagatcaa gatcgatggg aagcaataaa
    28561 gaaaagagaa agtaattttg ttaaaactta taataatcaa tgttctgcaa aaatcgttaa
    28621 aactgctatt cgcaataaag ctcaaattat tcgtatcatt gaaccagata aggcttatgg
    28681 cgaatgggct tactatcaat tgaaagaaca actagtaaac aaaggaaaaa gatatgggat
    28741 agaagtcaga agttagtaaa ttaaacaatc gttgatttgg caacgaaata gtgagggaaa
    28801 atccactcac tgaagggtaa actccgtcaa gttgtgtgac cagacggaag gacgaatgtt
    28861 gttgaggttt tagaaccata taaaacagca tgtttctcaa atttttagga taagggaaat
    28921 tgctagcagt ttgttttaga accatataaa acagcatgtt tctcaaatga gtactttaaa
    28981 ttaattgatg gcgttcctgc tttagaacca tataaaacaa catggattta aaacaagaag
    29041 taagtcagtt ctccttttac tttagtaagg aaaaataaag tattctcaat aaaacttaaa
    29101 ataagcaaac ctttgtttta atagctaatc gaagtaaaac ccaaagccat atgaacccct
    29161 gaatcagcga aagtttatga tgatttggat tactaatagt ttaggaatta gatggtaaat
//
LOCUS       DACCQI010000022        29103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCQI010000022 DACCQI010000000
VERSION     DACCQI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.022"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1030)
                     /locus_tag="IVO76_000763"
     CDS             complement(<1..1030)
                     /locus_tag="IVO76_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7428746.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IVO76_000764"
     CDS             complement(1112..1801)
                     /locus_tag="IVO76_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7428747.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IVO76_000765"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IVO76_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7428748.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IVO76_000766"
     CDS             complement(2328..2831)
                     /locus_tag="IVO76_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7428749.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IVO76_000767"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IVO76_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7428750.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IVO76_000768"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IVO76_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7428751.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IVO76_000769"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IVO76_000769"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7428752.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IVO76_000770"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IVO76_000770"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7428753.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IVO76_000771"
     CDS             complement(6443..7462)
                     /locus_tag="IVO76_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7428754.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IVO76_000772"
     CDS             complement(7635..8522)
                     /locus_tag="IVO76_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7428755.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IVO76_000773"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IVO76_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7428756.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IVO76_000774"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IVO76_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7428757.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IVO76_000775"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IVO76_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7428758.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IVO76_000776"
     CDS             9753..10397
                     /locus_tag="IVO76_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7428759.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IVO76_000777"
     CDS             complement(10453..10956)
                     /locus_tag="IVO76_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7428760.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IVO76_000778"
     CDS             11553..12518
                     /locus_tag="IVO76_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7428761.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IVO76_000779"
     CDS             complement(12617..13789)
                     /locus_tag="IVO76_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428762.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IVO76_000780"
     CDS             complement(13776..14921)
                     /locus_tag="IVO76_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428763.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IVO76_000781"
     CDS             complement(14935..15867)
                     /locus_tag="IVO76_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428764.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IVO76_000782"
     CDS             15984..17063
                     /locus_tag="IVO76_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7428765.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="IVO76_000783"
     CDS             17060..17689
                     /locus_tag="IVO76_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7428766.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="IVO76_000784"
     CDS             complement(17799..18026)
                     /locus_tag="IVO76_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7428767.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="IVO76_000785"
     CDS             complement(18129..20717)
                     /locus_tag="IVO76_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7428768.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="IVO76_000786"
     CDS             complement(20737..21396)
                     /locus_tag="IVO76_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7428769.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IVO76_000787"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="IVO76_000787"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7428770.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="IVO76_000788"
     CDS             complement(22169..23035)
                     /locus_tag="IVO76_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7428771.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="IVO76_000789"
     CDS             23144..23785
                     /locus_tag="IVO76_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7428772.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IVO76_000790"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="IVO76_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7428773.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="IVO76_000791"
     CDS             complement(25226..26125)
                     /locus_tag="IVO76_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7428774.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="IVO76_000792"
     CDS             complement(26127..26924)
                     /locus_tag="IVO76_000792"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7428775.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="IVO76_000793"
     CDS             complement(26902..27606)
                     /locus_tag="IVO76_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7428776.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="IVO76_000794"
     CDS             27737..28312
                     /locus_tag="IVO76_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP7428777.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..>29103
                     /locus_tag="IVO76_000795"
     CDS             28430..>29103
                     /locus_tag="IVO76_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7428778.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLS"
BASE COUNT         8561 a         6169 c         5232 g         9141 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taa
//
LOCUS       DACCQI010000023        28097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCQI010000023 DACCQI010000000
VERSION     DACCQI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.023"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            579..1286
                     /locus_tag="IVO76_000796"
     CDS             579..1286
                     /locus_tag="IVO76_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7428779.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1345..1989)
                     /locus_tag="IVO76_000797"
     CDS             complement(1345..1989)
                     /locus_tag="IVO76_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7428780.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            2115..2402
                     /locus_tag="IVO76_000798"
     CDS             2115..2402
                     /locus_tag="IVO76_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428781.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2510..2830
                     /locus_tag="IVO76_000799"
     CDS             2510..2830
                     /locus_tag="IVO76_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7428782.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2911..4224
                     /locus_tag="IVO76_000800"
     CDS             2911..4224
                     /locus_tag="IVO76_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7428783.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            4221..4940
                     /locus_tag="IVO76_000801"
     CDS             4221..4940
                     /locus_tag="IVO76_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP7428784.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            5068..5619
                     /gene="raiA"
                     /locus_tag="IVO76_000802"
     CDS             5068..5619
                     /gene="raiA"
                     /locus_tag="IVO76_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7428785.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5841..8375
                     /gene="secA"
                     /locus_tag="IVO76_000803"
     CDS             5841..8375
                     /gene="secA"
                     /locus_tag="IVO76_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7428786.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8501..9602
                     /gene="prfB"
                     /locus_tag="IVO76_000804"
     CDS             join(8501..8563,8565..9602)
                     /gene="prfB"
                     /locus_tag="IVO76_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7428787.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9707..10393
                     /gene="ftsE"
                     /locus_tag="IVO76_000805"
     CDS             9707..10393
                     /gene="ftsE"
                     /locus_tag="IVO76_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7428788.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10377..11270
                     /locus_tag="IVO76_000806"
     CDS             10377..11270
                     /locus_tag="IVO76_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428789.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11422..12870
                     /gene="cls"
                     /locus_tag="IVO76_000807"
     CDS             11422..12870
                     /gene="cls"
                     /locus_tag="IVO76_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7428790.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEIAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYILQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13207..14064
                     /locus_tag="IVO76_000808"
     CDS             13207..14064
                     /locus_tag="IVO76_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7428791.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14179..15099
                     /gene="pstC"
                     /locus_tag="IVO76_000809"
     CDS             14179..15099
                     /gene="pstC"
                     /locus_tag="IVO76_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7428792.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15099..15983
                     /gene="pstA"
                     /locus_tag="IVO76_000810"
     CDS             15099..15983
                     /gene="pstA"
                     /locus_tag="IVO76_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7428793.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15998..16804
                     /gene="pstB"
                     /locus_tag="IVO76_000811"
     CDS             15998..16804
                     /gene="pstB"
                     /locus_tag="IVO76_000811"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7428794.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16820..17578
                     /locus_tag="IVO76_000812"
     CDS             16820..17578
                     /locus_tag="IVO76_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7428795.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17591..18268
                     /gene="phoU"
                     /locus_tag="IVO76_000813"
     CDS             17591..18268
                     /gene="phoU"
                     /locus_tag="IVO76_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7428796.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18505..19536
                     /locus_tag="IVO76_000814"
     CDS             18505..19536
                     /locus_tag="IVO76_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7428797.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTNQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKHGLFALGSLIINIVLLLLLLLIFTKIEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNLWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19533..20300
                     /locus_tag="IVO76_000815"
     CDS             19533..20300
                     /locus_tag="IVO76_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7428798.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTTIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20400..21968
                     /gene="liaX"
                     /locus_tag="IVO76_000816"
     CDS             20400..21968
                     /gene="liaX"
                     /locus_tag="IVO76_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7428799.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22268..22585
                     /locus_tag="IVO76_000817"
     CDS             22268..22585
                     /locus_tag="IVO76_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7428800.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            22585..22935
                     /locus_tag="IVO76_000818"
     CDS             22585..22935
                     /locus_tag="IVO76_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7428801.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23102..24040
                     /locus_tag="IVO76_000819"
     CDS             23102..24040
                     /locus_tag="IVO76_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7428802.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            24055..24885
                     /locus_tag="IVO76_000820"
     CDS             24055..24885
                     /locus_tag="IVO76_000820"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7428803.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24929..25954
                     /locus_tag="IVO76_000821"
     CDS             24929..25954
                     /locus_tag="IVO76_000821"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7428804.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHCLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPVEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25969..26907
                     /gene="galU"
                     /locus_tag="IVO76_000822"
     CDS             25969..26907
                     /gene="galU"
                     /locus_tag="IVO76_000822"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7428805.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            27032..27472
                     /locus_tag="IVO76_000823"
     CDS             27032..27472
                     /locus_tag="IVO76_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7428806.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27475..28077
                     /locus_tag="IVO76_000824"
     CDS             27475..28077
                     /locus_tag="IVO76_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7428807.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIIIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT         9293 a         4839 c         6082 g         7883 t
ORIGIN      
        1 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
       61 ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac
      121 cttgttcctt gaagattctg ctgatttaag atagagttaa gttaactaca agaattggag
      181 ggatccacat gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg
      241 tcaaatcaga aaaatacaat attgaagtga atattccgaa aaaaggcgga aaagcggtcc
      301 gggtaaaatc aaaacgccca acgaatcata caaaaaaatg gatggctaaa aaccgctaat
      361 aataacaaaa gatccataag tcggcatttt catcgatttt agggtctttt gttattttct
      421 aggttggaca tatctaaaaa attgtttctg acaataggag taggaagaac gatgtgtgtc
      481 taaatcctct gttgatggat agaaaccttt atctttttca gttgtgaatc aggtatatac
      541 cagttataat agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat
      601 atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag
      661 accgtcttcc ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt atgacgttga
      721 gacaagcaat tcaaaacttg gcagatgaag gaatcttaga aagaaagatc ggttcgggta
      781 catatgttgc acgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa
      841 ttatggaatc acaaaatcga gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc
      901 caagctcaag tgaaatggaa aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg
      961 aacgtattcg tttcgcagat gatgtcccta tttgttttga agttgccagt gtgccacaaa
     1021 agctgatcga tggctacagc aaagcagaga ttactgaatc gttgtatcgc acgctagaag
     1081 aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat tactgctgtg ttggcttctg
     1141 agaaaattgc tgactattta gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga
     1201 tttcttattt tgaagacggt acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc
     1261 gttttgagtt ttatttggaa aaataaaaaa caggtgatcc ctgattccgt attagaaata
     1321 aaagagaatc agaatcacct gtttttatat atgatcagtt accagttgct cgtgataaga
     1381 aacaggaccc tcttcaaatt ggacttgtcc gttcagcagc tcaatgatgg attctttaaa
     1441 gtggtcagca cttttttcat ctacagcaca gatgacgcta acttgatcag tatattggat
     1501 ttcttttata aaaaaatgtt ggagttcaag ataattctgt aattttccta gttgcggata
     1561 cgtgatcatc acagagatct cctgttgcaa ggtaccttct acgattccaa tgtcagacaa
     1621 tgcttgagag accgcatgag tgtatgctcg tattaatccg cctgtgccga gtttcgttcc
     1681 tccaaaatat cgagtgacta ctgcagctac attgatcagt tgattttttt tgaggacttc
     1741 caacatcggt atgcccgccg ttccacttgg ttctccatca tcgctgcttc gctggatttc
     1801 atttttttct ccaatgatga aggcgctgca gttatggttc gctttccaat gttctttttt
     1861 tatttcttga atgaaacttt ttgcttcgtc ctctgattct attcgtctaa gagaacagat
     1921 aaagcgtgat tttttgatct cgatctcact ttgtccattt tcttttattg tacgatagcg
     1981 ctcgatcatc ttgtcacctc ttttttcatt ttctttattc agtataaata aagttctagg
     2041 ttgtttcaac actgtaacgg ttgcgcaatt gctgtagtat gcgataataa agacaaaggc
     2101 aggtggatct ggggatgcag gaacgaatga aaaagtacga tgcaattacg cattatttga
     2161 agaacaatgg cggctctcaa gtaacgttga cctttaccca atttgacgaa ctgctgtttc
     2221 ccagtaacgg acttcccaaa acagcaaggg aaagtacaga ttggtgggca aatgattata
     2281 aacaccctga aaaaggtgcc tatggctgga taaatgcagg ctatgaagta gttgtcatca
     2341 atttggacaa agaatatgtc gtgttcaata aattagtgaa atctagttgg ctatttgatt
     2401 aaataaaccg cgtttagaaa acgttgacaa atttctttct ttttatataa tagagtaaca
     2461 gacgcgaaga aaacgtttgc gtagtttgtt ttaaaaagga gagaaaagta tggctaaaaa
     2521 aacaatcatg cttgtatgtt cggctggtat gagcacaagt ttactagtaa caaaaatgca
     2581 aaaagctgct gaagcaaaag gaatcgactc tgatattttc gctgtgtccg cttctgacgc
     2641 agataacaat cttgcaaata aagacgtgga tgtgttgtta ttaggcccac aagttcgctt
     2701 tatgaaagct gattttgaga aacgtctgga accaaaaggc attcctttag atgtcatcaa
     2761 tatggctgat tatggtatga tgaatggcga aaaagtgtta gatcaagcaa tcacgctcat
     2821 aggtgaataa atagaacagt ataactatcg aacaagcgat ccaaaaaata gatcgcttgt
     2881 tttttcgtat atttttttag ggcgaataat atgaatcatt tttggggtag aaggcttctg
     2941 acgagagata ttaaagcaat agactgtgag gaggtaaatt taccaaatta tgagaagatc
     3001 gcagcagtgg aacgtttgag caactggatt ctttgtcatc gctgtggaat gagaacgtcc
     3061 gtcattgatg gacagcttgc agattacggt tatttttgta cacattgtct atatttaggg
     3121 cgatgcgaca ctcaacaaga tttatatttg tttgatcagc ctgaagcaaa acctcgagaa
     3181 gtcgtctttt tttggacagg acagcttact gaaaaacaaa cggaaatctc aaagagaata
     3241 ctttgccatt cagaaaaaag acatcatctt atttgggctg taacaggtgc agggaaaaca
     3301 gagatgcttt atcctatttt agtggaaact ttaaaagctg gaggaagagt agcgatttgt
     3361 acaccgcgag tcgatgtttg taatgaattg tttttacgct atcgttatgt ttttccagaa
     3421 gaaaaaatca cgttgctcca tgggaataca gcaacggctt atacctttac ttcttttgtt
     3481 atctgtacga ttcatcagct ataccgtttt tatcggtcct ttgatttaat agtgatcgat
     3541 gagatcgatg cgtttcctta taacggggat gcaggattag ctcatgctgt gcaaacagcg
     3601 attaaaccag acggacgatt catctactta agtgccacac ctgataaaaa gttactaaag
     3661 gagatcaagc agacgtttga catccacaaa cttgctttac gctttcatcg gcgtttgctt
     3721 cctgaacccg tcttggtttt catgaataat tggcgacaaa aatgcaagaa caagaagaag
     3781 ataaaatctt tggtgcggat cattcatcaa ttaattaaag aaaaccatat ccttttattt
     3841 tgtccaagta tcacgatgat gcatcgtttg aaagccacac ttcaagaggc tcttccgaga
     3901 tatgagatca cagatgtttc ttcaagagac aatcagcggg cggaaaaagt ccagaaaatg
     3961 cgagaagcaa aatatgacat actgttaacc acgatgatcc ttgaacgtgg agtcacattt
     4021 gaaagaatat ccgttatcgt tttaggagcg gatcatccgg tttttactaa atcttctttg
     4081 gtacaaatcg caggaagagc cgatcgtaaa gggccttata cgaacagtca agtatatttt
     4141 ttctatgaag aaaaaactgc agctattcga aaagcttgtc aggaaatcaa agaaatgaat
     4201 gaagaaggga agaagatact gtgaggtgca gttgctgcag taaagatatc gtccaaaatt
     4261 taacattcag cgagattatt ggtgtttcga aatacagatt acctcagact tgttcagttt
     4321 gtcgcgcaca atttcattta ttggaaacaa gaggctgttc tggctgtcaa aaaccaatcc
     4381 atttcaatct agagagtacg aaaaagaaag taaattctat ccccgtttac tgcagtgact
     4441 gtcaagaatg gaagaagaaa tatccgacct atgattttca gcaccgtgcc ttttttgtgt
     4501 acgatgaaaa tatgaaggcc tggctcaatc aatacaagtt tatgggagat atccgactag
     4561 caggcacctt tgcatataaa tggacgcaga taaaaaaaga atacccgaca tacatctatt
     4621 gtccaatacc tttatctgaa aaaaggcgaa cagaaagagg attcaatcaa gtaagcgaga
     4681 tgttgaaagc agcaagaatt ccttttcaga tgctactcaa aagagagaag catctgctgg
     4741 ctcaagcaaa aaacacgcga aaggaaagat tggagatgcc tcaacctttt gaacttgcag
     4801 tcaccttttc taagatacaa aataaaaaaa tattgattat tgatgatgtc tatacaacag
     4861 gacagactct tttccacgca gcagattgtt tgcagatcgc acagccgaaa atcatccgaa
     4921 ctatttcttt ggcgagatga attagatgtg gaaatttgat agcgatttaa tttgcaaata
     4981 acctagagtt cgatataata gaattaagaa gagggaaaga gtggtccttc tccttcttga
     5041 ttgtggtaga cgaaaggggt aatttttatg tttagatata atgtacgcgg cgaaaatatc
     5101 gaagtaactg aagcgattcg cgactacgtt gagaaaaaag taggcaaact agaacgctat
     5161 ttcagtgacg cacccgatgc aacggcgcat gtgaacttaa aagtttatac agaaaaaaca
     5221 gcaaaagtgg aagtaacgat cccattacct tatcttgtat tacgtgccga agaaacttct
     5281 ccagatttat acgcaagtat cgatttggtt gttgacaaat tggaacgcca aatccgcaaa
     5341 ttcaaaacaa aaattaatcg taaatcccgt gaaacgggca tgaatactgc aaatgctgct
     5401 gctttattca atgaagagga cgaaaacgaa aatgattctg aattagatat cgtacgtacg
     5461 aaacgccttt cattgaaacc aatggatagt gaagaagcag tcttgcaaat gaacatgctg
     5521 ggacataact tctttatttt tgaagatgca gaaacaaatg gtacaagtat cgtttaccgc
     5581 cgtaaagatg gaaaatacgg actgattgaa acagactgat cagccttact taaataacaa
     5641 cgaaaacttg cagcagctac tgacagctgc cgcaagtttt tttgagtttc ctcctctttt
     5701 agatgaaata tgaataaatt atgaagttct tactttaata cgcttaagat atttcaatta
     5761 tctataacta atgataaaat gaaaagtaaa gtctacatag tctggataat acacagaagg
     5821 aaaaggagat tacacaacgc atggccaatt tgattcgttc acttatcgaa aacgataaga
     5881 aagaactaaa acgtttagat agcattgcaa aaaaagttga agcatacgct gaccaaatgg
     5941 cagcattaac tgatgaacaa ttacaggcaa aaactgctga attcaagggc agataccaaa
     6001 aaggagaaac attagatcaa ttacttccgg aagcttttgc agtcgtccga gaagcagcaa
     6061 aacgtgtatt aggtttatat ccataccacg tacaattgat gggtggaatc gtgcttcacg
     6121 atggaaatat ccctgaaatg cgtactggtg aaggtaaaac cttgaccgca acgatgccag
     6181 tatatctaaa tgctctgact ggcgaagggg tacacgtagt taccgtcaat gaatatttag
     6241 caacccgtga ctcgacggaa atgggcgaac tttataattt cttaggatta acagtggggt
     6301 tgaatatcaa ctcaaaatct tcggatgaaa aacgcgaggc ttataattgt gatatcactt
     6361 atagtacgaa taatgaatta ggatttgact acttgagaga caatatggtc gtttaccgcc
     6421 atcaaatggt acaacgtccg ctgaattatg ccattgtcga tgaagttgac tcaatcttga
     6481 ttgatgaagc gagaacgccg ctgatcattt cagggcaagc agaaaaatcc acagctttat
     6541 atacaagagc agacaacttc gtgaaacgtt tgaaagaaga agaagattat aaaatcgatg
     6601 tacaatcaaa aacaatcagt ttaaccgaag cagggatcga aaaagcagaa gaaacattcg
     6661 gtttggataa cttatacgac attgaaaata ctgcattgac ccatcacttg gatcaagcat
     6721 tgagagccaa ctatatcatg cttttagata tcgattatgt tgtccaagac ggaaaagtac
     6781 tgattgttga ccagttcact ggtcggatca tggatggacg ccgttattca gatggacttc
     6841 accaagcaat cgaagctaaa gaaggcgtag agatcgaaga cgaaacgaaa accatggcga
     6901 cgatcacttt ccagaactat ttccgtatgt acaagaaact tgcgggaatg actggtacag
     6961 ctaaaacaga agaagaagaa ttcagagaaa tttataacat ccaagtatac cagattccga
     7021 caaatcgtcc ggttatccgt gatgatcgtc cagatttgct ttacccaaca ttacaaagca
     7081 agttccacgc tgtcgtgcaa gatatcaaag atcgctaccg caaaggtcaa cctgtgttag
     7141 tcggaactgt tgcggttgaa acatctgagt tattatcaga tatgctgaat caagaacgta
     7201 ttcctcatga agtgctgaat gcgaaaaatc actttaaaga agcagagatc atcatgaatg
     7261 ctggtcaaaa aggtgctgtg acgattgcta ccaacatggc tggtcgggga acagatatca
     7321 agttaggact tggggtaaga gaacttggtg gtctagcagt tatcgggaca gaacgacatg
     7381 aatctcgtcg tatcgataac cagcttcgag gacgtgcagg acgtcaagga gaccctggta
     7441 tgtcacagtt ctacttgtca ttggaagatg atttgatgaa acgattcggt tcagaacgaa
     7501 tcaaagcttt cttagatcgg atgaagatcg gtgaggaaga tgctgttatc caaagcaaga
     7561 tgctgacgaa acaagtagaa tctgcacaaa aacgtgtaga aggaaataac tatgacacac
     7621 gtaaaaacgt cttacaatac gatgatgtga tgcgtgaaca acgtgaagta atctataaac
     7681 agcgacaaga agtaatcatg gaagaaaaaa gcttatccaa gccactaatg aacatggtca
     7741 aacggacaat cagtcgtgta gtagatgcgc atacacaatt agagaaagaa aattggaatt
     7801 tggaaagtat catcgatttt gccggcagta ctcttgtcca tgaagatagt attactcttg
     7861 cagatatcga aggaaaaaca gctcaggaga tcaaagagta tctaaacgaa cgagcacaag
     7921 aagtatttaa taccaaagcg gctcaactaa atggtccaga gcaactgttg gaatttgaaa
     7981 aagtcgtgat cttgcgtgtt gtggactcta aatggacaga ccatattgac gcaatggatc
     8041 aattacgtca gtcaatcggc ttacgtgctt atggacagaa caatccatta gtcgaatatc
     8101 aaacagaagg ctataagatg tttgaagata tggtaggcgc catcgagtat gaagtcactc
     8161 gaatctttat gaaagctgag atccgtcaaa acgtacagcg tgaacaggtt tctcaaggaa
     8221 cagcttctca ctcagaagac ggagatgcca aagaaaatag taatactagc gcgaaaaaac
     8281 agccaatcca tgtgaacaag gtcggaagaa atgatccttg tccttgtgga agcgggaaaa
     8341 aatataagaa ctgtcatgga aaaaatgcaa actaatttct gacagcagga ttggtgagac
     8401 aaattctttt tttaagaatt cgtctcacct tctttctgtt atttgctgat tctgttagac
     8461 tagatataat gaaaaaagta tagtgaaaga aggaaaaaac atggaaatat ccgaaattcg
     8521 aaatctcctg acagaaatgc aggaaaaggt cacaagtttc aggaggtctc tttgacttag
     8581 atcagctgga agaagacatc gcggaagcag aagcaaaaat ggctgaacct ggtttttggg
     8641 atgacagcga agccgcacaa aaagtgatca gtgaaaataa cgctaataaa gaaacctatg
     8701 accaattcca ttctcttgcc gaagaactag aagaaatcga attgatggtg gagatgctgc
     8761 aagaagaacc tgatgcagca atgcaagaag aagcagaaac acgtatcaat gcattagatg
     8821 aaaaaatgaa gacgtatgaa ttatctatgc tgttagacgg accttatgat cgaaacaatg
     8881 cgatcgtcga attgcatcca ggggctggcg gaacggagtc ccaagactgg ggcagcatgc
     8941 ttttacgtat gtatacacgt tgggcagagc agcatggcta tcaagtagaa acactagatt
     9001 atcaagcagg tgatgaagca gggatcaaga gtgtgaccct gctgattaaa ggacataacg
     9061 cttttggtta tctaaaatca gaaaaaggtg ttcatcgcct tgtacggatt tcgccttttg
     9121 actcagcgaa acgacgtcat acttcttttt gttcggtaga tgtgatgcca gagcttgatg
     9181 aaacgatcga tatagaaatc aatccagatg atttgaaaat cgatacgtac cgagcaagtg
     9241 gtgcaggtgg tcagcacatc aataaaacag aatctgctgt acggatcact cacttgccta
     9301 caggaacagt agtagctagt caggcacaac gctcacaatt gaaaaaccgt gagcaagcga
     9361 tggggatgtt aaaagctaag ctttatcaat tagaagtgga aaagaaagaa caagaagcag
     9421 ctgcacttcg aggagaacaa aaagaaatcg gctggggttc acagatccgt tcttatgttt
     9481 tccatcccta ttctatggta aaagaccatc ggacaaacgt agagacagga aatgtccaag
     9541 cagttatgga cggtgacctt gatttattta ttgacggcta tttaaaaatg catctacaat
     9601 aagaacccat tgttaaattg aaataaaatt gtaagatttt gcaagcatgt tgaaacattg
     9661 ccatgctata atagctaaag actgaaaaag atatggagag aacgccatga ttgaaatgaa
     9721 ggatgtaatg aagaagtact caaatggtac gaccgctatc cgcaatctct cgattgtcat
     9781 cgaacaagga gagtttgtct atgtggttgg tccgtctgga gccggtaaat ctacattcat
     9841 caaattgatg tatcgagaag aaaaagcttc aaaaggaact ttgaacgtcg caggtcatga
     9901 tctaatagca atcaaaaacc gcgaagttcc ttatctacgc agagaaatcg gcgtggtgtt
     9961 ccaagactat aaattattac caagaaaaac agtatatgaa aatgttgcgt acgctatgca
    10021 agttatcggc agaaaacctc gagatatcaa aaaacgagta atggaagtgc tggaccttgt
    10081 tggtttgaaa cacaaagttc gagtctttcc tagcgaatta tccggtgggg aacagcaacg
    10141 tgtctctatt gctcgggcaa tcgtgaatac gcctaaagta cttatcgcgg atgaaccgac
    10201 aggaaatctg gatccagaaa actcatggga aatcatgaaa ttattagatc ggatcaatgc
    10261 ccaaggaact actgtcgtga tggctacaca taacagtacg atcgtaaata cgattcggca
    10321 ccgagtaatt gctatcgaaa acggccgcat tattcgagat caagcggaag gggaatatgg
    10381 atacgatgat tagaaccttt ttccgccatt tattagaaag tatcaagagt ttgaaacgta
    10441 acggctggat gacagttgct tcagtcagtg cggtaacgat cacgttgact ttagtcggtg
    10501 tattcatggc agttatcatg aatgcaacta aattagctca ggatatagaa ggaaacgttg
    10561 atgtatctgt attcgtagat atcggtacaa aagacgaaga gatgaaaaag ctagaaaaag
    10621 aattgaatga cttagatcat gttaaaaaag ttgaattttc aagtaaagaa caggaattga
    10681 aaaaaatcca aaacgaaatg ggtgattcct ggaaattgtt tgaaggtgac aataacccgt
    10741 tatatgatgt gtatgtagta agtgctactg atccttctta tacaaaacaa gtagcagaag
    10801 aagcagcaga tttgaagaat gtttctcgtt ctgattacgg tggagcttcc tcagatcgga
    10861 ttttccaaat cgctggtgca gttcggacat ggggattagt tgcagcggca ttgctgctgt
    10921 tcgtagcgat gtttctcatt tcgaacacga ttcggatcac gattttgtct agacagcgtg
    10981 aaattcaaat catgcgttta gtcggtgcaa aaaatggata tattcgctgg ccgtttttcc
    11041 ttgaaggtgg ctggatcggt ctgatcggtg cgattcttcc gattttgatc atcacatttg
    11101 gttatgcatg gtttttccaa ttgatcaatc catcattatt acgttcccat tattcattga
    11161 ttcatccaca aaatatcgtt tggaaaatca atcttttaat ggtcggcatt ggtgtgatca
    11221 ttggttcact tggctcgatc atttctatga gacgtttctt gaaattctaa taccacaata
    11281 taaataaaat gagaaagttg gacagtttcg ttccagcttt ctttttttat tggctaggag
    11341 aatagaagga agcggctttt ttccctttac tttaaaaaac aacttgggta ttcttaagtt
    11401 ggtagaggaa gaggtgaagc cgtgaaaatt atcgttgaca atttttttac gatccttttg
    11461 attatgaata ttttgctttc ctttatcatc gtttttcgag aaagaaagga aactgctcaa
    11521 acatgggcat ggctgctcgt tttgatgttc attccagttg taggatttat tttatatatt
    11581 tttttaggaa gagggatttc caaagagaaa atctttgatt tgaagatcca agggaaaatc
    11641 ggtaagaacc tggaaattga agaagacaaa caggcattga tgcgagggct ttatcctcat
    11701 ccgcctactg gacaagtaga tgtgaaacaa ttgatctata tgctcacggt atttgaaagt
    11761 acgctctata cgactaaaaa tgaaatcact ttatttacag atggtcgtga aaaattcgat
    11821 gcgctgattg aagatatcaa gcaagcagaa gaccatatcc acttccagta ttatatttat
    11881 cgcagtgatg cattaggtga agaagttcga gatgcattga cagaggcggc aagacgaggg
    11941 gtaaaagtca gagtccttct tgatgcatgg ggttccacac aagtttcttc cagctttttt
    12001 cagaatttga aaaaagcagg cggagaaatc gcatttttct ttcctctatt tgttccgtat
    12061 attaatcctc ggatcaatta tcgcaatcat cgtaaaatcg tagttattga tgggaaaatc
    12121 ggatatacag gtgggttcaa tgttggaaat gaatatctag gattggtaaa gaaatttggt
    12181 tattggcgag acaatcattt gcgtatttac ggagaagcgg tgtatatctt gcaaaaccgt
    12241 tttttgatgg actggaattc tcagcataca aaagaagtag gctattctcc caaatttttt
    12301 ccgtccattc actctactgg tgatatagct gttcaaatcg tgacaagcgg accagatacc
    12361 gaacatgaac aaatcaaaat gacttatttg aaaatgattt cgttagccaa acgggagata
    12421 ttgatccaaa cgccttatta cataccggat ggatcgattc atgaagcact gaagttggct
    12481 cttctttcag gcgtaaaagt tcatatccaa atacctaaca aaccagatca tcttctcgtt
    12541 tactgggcaa cgtattcttt cgctgcagag ctgatcgagt atggagcaag gatcgaaaca
    12601 tatgagaatg gatttattca tgcaaagacg atgattatcg atggtggcat tgtttcagtt
    12661 ggctcagcaa atatcgatgt tcgttcattt cgcttggact ttgaggtcaa tacactgatt
    12721 tatgatgaac gaatggcttc acgtgtaaga aaagcatttt ttgaagactc gaaaatttct
    12781 acgcatctca caaaagaaat gtatgaaaac cgaggaataa tcatcaagat gaaagaagga
    12841 ttagcaagat tgatttcgcc gctcctttaa aacacaaaaa ggacatgaga accatcaaca
    12901 gatggagatc tcgtgtcctt ttctaacggg gccatttctg agagtacgtc agaaatggag
    12961 aaagcgtctc gcatgagcga aattaacttt ggggtatctc ttgttttttg gggaaagcaa
    13021 gaaataagct aatatatgct attaaaagag acacatttca aagccagtat agcatagaaa
    13081 cttttaatga gatagttttt tttacaatta tgaggattta ttttgttatt taatgatttt
    13141 gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga ttaaaggaga
    13201 tttctaatga aaaaacttaa attattctta ccaataatag gattagctat tcttttatca
    13261 ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc ttctgcttta
    13321 cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg aaaattcatc
    13381 aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg agcagtcgac
    13441 atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc agcattagtc
    13501 gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat tggcgtaaaa
    13561 gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa ctggagtgaa
    13621 gtcggtggaa aagatcagcc agtagttata ttgaaccgtg cttcaggaag cggaacccgt
    13681 agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca agaacaagat
    13741 tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag ctatgttgct
    13801 ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga accgacagat
    13861 gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta cacaagagat
    13921 catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga agtacaagat
    13981 aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga acgtgattgg
    14041 caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag ctaaactttt
    14101 acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat gttaaacctg
    14161 attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa atcaaaacgt
    14221 tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct gatcgtgttg
    14281 gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt ttttactgat
    14341 aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca aatcggagca
    14401 aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat tgtgaccttt
    14461 ttatctgcta ttgttgccac accatttgcc attggtgcag cagtattcat gacggagatc
    14521 tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt aggtattcct
    14581 tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag aagtattttt
    14641 ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat gatcttgccg
    14701 actgtcacaa cgatgactgt tgatgcatta aaggcagtac cacggcacta tcgggaagct
    14761 tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct acgagcagcc
    14821 gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg tgaagcctta
    14881 gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac tactcctgct
    14941 tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg aacagttgaa
    15001 aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt ctttaatatc
    15061 gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa cgctcagata
    15121 aaattgctac aggtgtttta tatgtagttt cggggatcat cgtattgatt ttagcagctt
    15181 tactacttta tatcttagtt cggggaatac cgcacatttc atggtccttc ttgaccgaac
    15241 cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac tcgatttatt
    15301 tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt atctatcttt
    15361 cagaatatgc tggaaaaaac tggctgacag atgtgattcg cacgtcgatt gaaatcttga
    15421 gctcattgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt attcaattcc
    15481 agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac ttgcctcttt
    15541 tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga gaagcaggat
    15601 tggctttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca gaagcgacac
    15661 cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa gcagctgcat
    15721 tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg aatccattaa
    15781 gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc catatttgga
    15841 agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc gcttctgccg
    15901 tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc aaacgattat
    15961 accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata actggaatga
    16021 aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg atgatctaca
    16081 tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg aaaaaaataa
    16141 gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc gttcgttgaa
    16201 tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt atcaaggcgt
    16261 agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg gcatggtttt
    16321 tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg ctttgaaaca
    16381 gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct tgaaacaagc
    16441 tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt tatctggcgg
    16501 gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata tcttgctgct
    16561 agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag aaacacttgt
    16621 aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac aagcggctcg
    16681 tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg acaaaactaa
    16741 caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg gtcattttgg
    16801 ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc ttcatctgta
    16861 ttatggaaag aaagaagcac taaaagggat cgatctaagt ttcaatcaag gggaaatcac
    16921 tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt tgaaccggat
    16981 gaatgacctg attccgaatg taacgatcac cgggagtgtt ttatataaag acaaagacat
    17041 ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg tattccagca
    17101 gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga aattaaaagg
    17161 ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg cagcgtctgt
    17221 ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg gtgggcagca
    17281 gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct tattggatga
    17341 accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc tgttagaatt
    17401 aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt cacgtatttc
    17461 agataagacg gcattctttt tagatggtca cttgatcgaa tacaatgata cgaagaagat
    17521 attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat ttggataagg
    17581 aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc ataatcagtt
    17641 ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg catacatcaa
    17701 acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca ataacatgga
    17761 aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag ttacaactga
    17821 tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta tggccgatca
    17881 tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa taccggaaat
    17941 cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca acgtcctagt
    18001 tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg atcaaagagt
    18061 caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg aaaatcctga
    18121 gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac ggatcggaga
    18181 ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa tcactgagtt
    18241 aaatacgaac cacaatgaga ttttctaaac taaagcggtg attctgccgt ttgtttagga
    18301 aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta ataagaacag
    18361 tcaaacaaaa atagctcctc atatttttca tttggctgga cttattaccg cagatgcaag
    18421 cttttgaaca ctgtttattc gggaaagggt ttgtgacaaa atatgtcaca aacccttttc
    18481 atcatttcat agaaaggata aagaatgcaa aaaaaaatta gcatcatcac tatatttttt
    18541 atttgtgtgc ttacttcatg ggttattcag catcaagctg ctttatatga agtacctgtc
    18601 atcgagatcg aaaaagtacg aataaaagag gatcatcaat tgattacagg aagactgatg
    18661 aacacgaatc aaaaaggaga atcgatttat ttatcggttc cttatcaaaa aaatcaaata
    18721 gaaacagcaa atataaaagc gagacaaatg tatctagtcc aaatgcatgg agatgaatgg
    18781 cagctagtag caaaaaaaaa tgatggctgg cttttcttct tcacaagtat cttcattggc
    18841 gccatgttgc ttattggcgg aaaacatggt ctatttgctt taggaagtct catcatcaat
    18901 atcgttttac ttttactttt gttgctcatc tttactaaaa tagaagggat ttctctatta
    18961 aatatatcta tcctgttcgt cgttataggg atgattatta gcatattggc tttagatgga
    19021 tggaatagaa gcagtataat caaaatttca gctgcagtta cgagtgtttt tttagcattt
    19081 tttatttgtc ttttgacaat gaatctttgg aaagaccaag gactgcgcta tgaggaactg
    19141 aattatctga cacgaccgta tcgttctgtt tttctttcta gcgtactgat tggaacagct
    19201 ggaggaacac tcgataccgt tatcacagtc attgcgacat tagaagaatt gactaagcga
    19261 caaccagaaa taacagcgaa acagttatgg ctttcgggaa ggcaagtggg tcgagatgtg
    19321 attggctcga tgaccaatat ccttttgttc gtctatatca gtgggtcagt tccatcgctt
    19381 cttttatatt taggaaatca atggtcgttc aaagaaacga tagaacagca tttatcattg
    19441 gaatttttac gcgtcattgc tggcagctta ggaatagtat tatcgatccc tatctcggtc
    19501 ctcttttttc tgactgtaag gaggctgaaa cgatgaatgt acttattata cttgttggga
    19561 ttttagcaat cagcgtgcta cttattggcg gtacgcaagg tatgtatatt cttttaggct
    19621 tattcatcaa tttagggatc tttttcttgt tgttattcgg ttatcatcag aagtggccga
    19681 tactggcatt gtcggtcata ggatttttat tgattgctgt ggttatctta ttttttatca
    19741 acggttataa tctaaaaatg cgtgcagctt ttgtgagtat actgatcttt ttattttgtt
    19801 tcctactatt gcttcctatc actgattttt tagcaatcca aggttttaca acgatagaac
    19861 tagaagaatt atctggctta gataagactt tagcgattga ttttagatta cttacgagat
    19921 cgttgcttct gattagtctt agtggtgctg ttttagatgc gagtgtagca atttctagtg
    19981 ggacattcga agtatatcag gcaaatccgc aattatcttt ttaccaattg cgccattcta
    20041 gtttcgctat tgctaaaaaa atattggcgt caacagtaat gacacttctt tttgcattca
    20101 tgggaagttc tttagcgttg atcctctggt ttattgattt agacattcct tttcagcaag
    20161 tgatcaatga aaaatcattt gttctagaat atacgatggc tattcttacc acattgtccg
    20221 cactacttgt tcttccttta acctgcgtga ctacagcata tctctttaca aaaaagaaag
    20281 aacaactcaa gatggagtaa aagttacaac ttaagtccta tgacattttt actgaatcag
    20341 tgcataatat ggatgtaagg aaaagcttgc aaacaatttt gcttaaggag gcaatttcca
    20401 tgaaagaaag agaacgtatt ttagatttag tgaaaaaagg aatcctttcg actgaagaag
    20461 gcttagattt attagaaagc atggcaactg aaaaagacga aaagcaaatc aaaaaagaag
    20521 cagacaaggt aactgctagt cataaagaaa aagatcaggc aagtcaattg atcgataaac
    20581 ttgaaaacgg cgaagaagaa atttctgagc cagttgatcc aaaggaaaga gaaagacaag
    20641 atcaagaaaa cttagaaaag atcttagatg aactagcaac tgaagcgaat aaaacttcgg
    20701 cgcgtctaga tgaagtaaat gctcaattcg ctgacaataa ggcagcacgc aatgagaaac
    20761 aggaagcatt gatgcagctg aatacaaaag aagaattagg cgagttgaca gaagaagaat
    20821 tagcacagcg tcagacgtta gaagcagaaa tcaaagagtt agaagctagt ggcgatacac
    20881 tgttagaaga acaattgaaa ttagaagcag aattaaaaga tatccgtaag aatcaatggt
    20941 ctgagaaaaa agaaacgttt actgataagt ttgaattgcc agatgattgg aaagatcaag
    21001 caacagatac actgaatcaa gtaggcgaaa aaatgtcaga agctggatca cagttaggaa
    21061 aattcttgaa gaaaacattc caaactgttt cagaaacagt gaatgacaac atggaatgga
    21121 aagatgtcag cttgcgtgtc cctgggattg cgacaacgaa atttgagcat gaattttact
    21181 atgaagctcc tgcagcaagt atccttgata tcaaggcagc aaacggaaac gtgacgttaa
    21241 aaacgtggga cagcaacgat gtgaaagttg aagctaagat caaactttac ggaaaaatgg
    21301 gtgctgaacc atttgaagca ttctctgaac ggagccagat tgaggttaat gaagatcata
    21361 tctccttcca aattccaaat aaacgtgtgc gtgcagacct tgttttctac ttacctaaac
    21421 gagtatacga ccacgcagct attaaacttc taaatggcaa catcatgatt gaaacattgg
    21481 aagctaaaga tatttataca aaatctacga atggtaacat catcgtggac caattaactg
    21541 caacgatgct ggaagtagaa ggtgtaaacg gcaatatcga tattcgaaac gggaacatct
    21601 tagattctat tatcgaaaca gtaaatggta cagtgacatt tggtgcgaca gcagaaaacc
    21661 tttctgtgtc tttggtaaat ggtgatgtac gtcttacgat taaagaagat aatttgaaaa
    21721 aagtagaagc aagttcagtg aacggaaatg tcaaagtcgc tttaccagat ggcatcggtt
    21781 tggaaggaca tgctaaaaca agtcttggaa gtatcaacag ccgtctgtcc aattacgaag
    21841 tagttcgtga gaaaaaagaa cggacaaacc aaatgttgca attccgtcgt gtttcagatg
    21901 gtgaaatcgc gcaagtgcaa ctttcaacga caacaggaag tatctattta aaagatacag
    21961 ataaataaag aggttgtgaa agagatgttc agccttgagc aataagaagc gattttgtaa
    22021 aatagcttcc cctattttac aaaattgtgc cttattgccg ataggctatc tcttgaacac
    22081 cgtttaccaa gagattatga acttgccaat tatcgccgag tcacaaggaa taatttcaaa
    22141 aaaagctcgt cacgttttat gaatatggta atttgtgacc gagaattttg caaaaagaca
    22201 ccgattaaga agaaggttgt cacaacgatt ttcttcattt aaaatagtag aacagaggtg
    22261 agacatgatg aaaaaaaagt tgacaaaatc caataaaaat gttgttttga cagggacttt
    22321 agcaggaata ggagaataca tcgggattga tccgactgtt atccgtgtgc tttatgtatt
    22381 cctgagctta gttgcttttg gaagtccaat cattttatac attttattag ctttgattat
    22441 cccatctgcg cgttctgcac gaacaaacta tggacataac aatgcatatt atgaaaaaaa
    22501 caattatcgt gaacaaactc gtgaacaaac aaaaaaacgc aaagaagcgg aaaaaatcga
    22561 tgatgacgaa tggagtgact tttaatgtcg tactttcaac gtattttagt taatacgtta
    22621 acattcatat cattggctgt tctttttcct gcgatggtcc atgtgagcag tatttggacg
    22681 gccgttcttg ccagcttcgt attgtctgtg ttgaatatgt ttgtcaaacc gattttgacg
    22741 atactgtccc tgccctttac attagtcaca ttgggattgt ttacgtttgt tatcaatgca
    22801 gcgattctta aaatgacctc cttttttgtg ggagcagaac attttagttt ttcttcattc
    22861 ggtgcagcgt tgttagtagc tatcttgatg tcgcttgtca atgtaattgt cagcgaacat
    22921 aacagtagaa aataaaagaa agctgtgccg aactatcggc gcagtttttt tatttagaat
    22981 caggaatgcg ttttctatta cattggtaat taaataaaaa cgtttttttt aattggtagt
    23041 acttttggct agacaatgat aaaatgatag agaacactct aatagaaatg aggtagcatg
    23101 aatgtcagaa gttgtaaaag ttagccaatt agtgaaaaaa ttatctttgg aaatcgtgac
    23161 aggcgacgag aaaagcttag accgtgtcat cgtaactggg gacatctcac gcccaggact
    23221 agagttgact ggttatttta attattattc ccataatcgt ttacagctat ttggcagtaa
    23281 agaaatcagt tttgcggaac ggatgatccc agaagagcgt ttgatcatca tgagaagatt
    23341 atgtagtgaa gatatgcccg catttattat ttcaagagga ttaccagctc cagaagaaat
    23401 gatccaggca gcaaatgaaa atgggatagc tgtactgcgt tctcccatct caacttctcg
    23461 cttattagga gaattatcca gttatttaga tggtcgcctt gcccctcgaa caagtgtcca
    23521 cggtgtttta gtggatgttt acggattagg tgtactgatt caaggtgaca gtgggatagg
    23581 aaaaagtgaa acagcgttgg agttgatcaa acgcggtcat cgattgatcg cagacgatcg
    23641 agtagatgtt tatcagcaag atgaattaac ggtgatcgga gaaccgccta aaatcctgga
    23701 acatctgatt gaaatccgag gaatcgggat cattgatgta atgaatcttt ttggtgcgag
    23761 tgcggtacgt ggctctatgc aagtccagtt ggctgtttat ctggaagcat gggcaaaaga
    23821 taagaaatac gaccgtctgg gctctgaaga cacgaccgtt gaaatcgcgg aagtaggcat
    23881 tccacaaata aaaatccctg tcaaaacagg acgaaacgtt gcaatcatca ttgaagtagc
    23941 cgcgatgaat ttccgtgcac gtacaatggg ctttgacgca acgaaaacat ttgaagaaag
    24001 attaagtcgc ttgattgaag aaaactcagg caacgactag caaggagtga caatatgtta
    24061 gcacaaatca atcgagttgc ttttgagctt gtagggattc ctatctactg gtatgcattg
    24121 atcatcgtat caggtatcat tatcgccatg tggcttagca gcagagaggc tgttcgggta
    24181 ggattaaaag cagatgatgt gacagatttt atgttgtggg gattgcctat ctccattatt
    24241 ggcgctcgct tatattatgt tttatttgat cttccccagt atatagctga tccaatccaa
    24301 atattcaata tccgttcagg aggcttagct atttatggcg gactgattgc aggcgcgatc
    24361 acgctgtatt tttttactaa atatcatttt atttctttgt ggactttttt agatatcgct
    24421 gcaccaagtg tattattagc acaagcaatc ggtcgttggg gaaatttcat gaaccacgaa
    24481 gcatatggac cagacacgac gcgaagcttc ttagaaagtc tgcatttacc gcaattcatc
    24541 attgataata tgtatatcga agggatctat cgtcaaccaa catttcttta tgaatcactt
    24601 tggagtttga ttggatttat tttgcttttg tttttacgaa agaaaccaca ccttttgaaa
    24661 aaaggtgaag tccttttatg ttatctaatg tggtatagtt tcgggcgttt ctttattgaa
    24721 ggattacgta cagacagtct ttacttttta ggtgtgatcc gtatttctca attattatct
    24781 gctgtgttat ttgtcggtac attgattcta tttatttggc gcagaaaaaa aggaaattta
    24841 ccggattatg accgctcatt aggaaaaaat caacaaatta tctgattttt agagcgttaa
    24901 aaatgaaaaa aatttcagga gtggtaaaat gaattataga gtagcggttt taggaccagg
    24961 ctcttggggt actgctttgg cacaagtcct cgccgaaaac ggacatgacg ttcggatttg
    25021 gggacatcgt gcttcacaag tagaagaaat caacacccaa cacacgaatc aacgttattt
    25081 accagatcat tgtctacccg attccatcca agcattcacc aatatggaag atgcagtaaa
    25141 agacgcagat gcggtgctat tcgtcattcc aacgaaagca atacgttctg ttgcaaaaga
    25201 actagtgcct aaattgaata caaagccagt gatcatccat gcaagtaaag gattagaaca
    25261 ggggacacat aaaaggatct ctgacatctt gttagaggaa atcccagtag aaaaaagaaa
    25321 agctgttgtt gttctttctg gaccaagcca tgcagaagaa gtagcgaaac atgacatcac
    25381 tacgatcaca gctgcaagta cagatgcaaa agcagcaaaa tatgtacaag agctgttcat
    25441 gaataattat tttcggatct ataccaatcc tgatgtgatc ggtgtagaga tgggggcagc
    25501 tttgaaaaat attattgcta ttggtgctgg cgctattcac ggcttagggt ttggcgataa
    25561 tgcaaaggca gcaatcatga cgcgcggatt agctgagatc agccgtttag gtgtagcaat
    25621 gggtgccaac ccgctgacct ttatcggatt aagcggagta ggcgatctag tcgttacttg
    25681 cacaagtgtt cattctagga actggcgcgc aggcaacttg ttaggacaag ggcagcccct
    25741 agaagaggtg ctagccaata tgggaatggt cgttgaaggt gtgaatacta cccaagcagc
    25801 gatggaacta gcccaaataa tggaaatcga aatgcccatc actcaaacaa tctatgaagt
    25861 gctgtatgaa caaaaagaaa tcaaaacagc cgcaaaagaa atcatgttac gtgatgggaa
    25921 aaaagaaaat gaattctcat taacaatttt ttgaaggagc gaatgtatat gaaagtcaaa
    25981 aaagcagtta ttccagcagc aggattagga acaagatttt taccagcaac aaaagctatg
    26041 gcaaaggaaa tgttgccaat tgttgataaa ccaacaatcc agttcatcgt tgaagaagcg
    26101 ttgaaatcag gtatcgaaga catcttgatc gttacaggaa aagctaaacg accaatcgaa
    26161 gatcactttg attcaaatat tgaattagaa acaaacctaa gtgaaaaagg aaagaccgaa
    26221 ttattgaaat tagtagaaga aacgacagat gtcaacttgc atttcatccg tcaatctcat
    26281 ccaaagggtt taggacatgc cgttcttcaa gcaagagcct ttattggaaa tgaaccattt
    26341 gtcgttatgc taggcgatga tataatggaa gacgaagtac cattgacaca gcaattaatc
    26401 gatgattacg aacgtactca tgcttctacg atcgccgtta tgcaagttcc tcatgatgaa
    26461 acttcaaaat acggaattat caatccagga gaagtattgg aagatgggtt atacaatgtc
    26521 aaaaactttg ttgaaaaacc agatccaagc aaagcaccaa gtgatttagc tattattgga
    26581 cgttacttat tgactccaga aatttttgat gtattgtccg agcaacaacc aggagccgga
    26641 aatgaaatcc aactaactga cgcaatcgat acattgaata aaacacaacg cgtatttgcc
    26701 cgtaaattca caggtaaacg ttacgatgtc ggtgacaaat ttggttttat gaaaacaagt
    26761 attgaatatg gtctaacaca tccagaagta ggtgcacctt tacgtgagta catcctgtct
    26821 ttaggtaaaa cattagaagc agaaggaagc aagatcgatc ataatccaaa aggaataaca
    26881 gaaatcaaag aagaaaagaa aaaataaccc ttttttctaa cgagcttcag ctgctaattg
    26941 aataaaaagt agttgaagct cgtttttatt tgtttaaaaa acacgaaaat gctatcctta
    27001 tagaaaagag agacaagaag ggagtttgat catgtcagga ggagaaattg caggattgat
    27061 cgcagcaatc gcctttgcga tacttgtcgt ttttatcgtt cgtgtgttgt tgcaagtttc
    27121 taaaacttta gaaaaagtag atcagactgt agcagaagca aatacgacga tcgaaatcgt
    27181 cacaaaagac gtagatcttt tgtctagaca agtagaaggc ttactggtca aaagtaatga
    27241 actgctcttg gatatcaatg gaaaagtcgc aacgatcgat ccattgttca cagcagttgc
    27301 tgatttaagt gaaagtgtat cagaacttaa tcattctagt aaaaacatcg cgacgaaagt
    27361 cggcggaatc ggtaaaacga cagctaaagc gacggttgcc ggaaaagtcg gtgggacagc
    27421 gatgaaattt tttaaaaaca aaaaacatac tgaaacgaca ggaggaaaat aaaaatggct
    27481 aaaaaaggtg gatttttatt aggtgcagta gtcggaggca cagccgcagc aatcgccgcg
    27541 ttattgttag caccaaaatc tggtaaagaa cttcgggatg aattagctgc ccaagcggat
    27601 gatttgatgg atatggcttc agattacaca gatatggcga aagaaaaatc gactgagtta
    27661 acaggtattg caaaagaaaa agcgcaagat ctttctcaac aagcaggtga tctagcaggt
    27721 agtgtcaaac aaaaagtaag tgaatcagca gatgaagcag gaagtgaatc tgacgatatc
    27781 ttgttaaatc ttaaagaaca atcagctgac ttatctaatc gtttcaaaaa aacggcagaa
    27841 gatatgaaaa aacctgcaga tgaattagga agtattgcta aagatacttc agaagatatc
    27901 ataattgatg taaaagacgc tgctggtaaa gcaggagata ctgtcaatga aggaatgtca
    27961 gaagcaaaac cagttgcaga tgactcaaaa gaaatgatga aaatggcaaa agaagatgcg
    28021 aaagaagcta aaaaagaaat aaaagataca atgacagatt catctacaaa tcactaaaat
    28081 cacaaattta tagaata
//
LOCUS       DACCQI010000024        27623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCQI010000024 DACCQI010000000
VERSION     DACCQI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.024"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..903
                     /locus_tag="IVO76_000825"
     CDS             <1..903
                     /locus_tag="IVO76_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7428808.1"
                     /translation="ARKEFPHLQVEERSAVSIARRLQDPLAELVKIDPKAVGVGQYQH
                     DVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHISGLNKTTAQNIVIYREENGEF
                     TARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGIHPESYSIAKEILTSVQLSEK
                     ELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLTQPGRDMRDEMPAPLLRTDVL
                     SMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHISKLSKKFVKHPTDVVSVGDIV
                     TVWIEQVDTKKGRISLTMLSPYEE"
     gene            923..1405
                     /locus_tag="IVO76_000826"
     CDS             923..1405
                     /locus_tag="IVO76_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7428809.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            1421..2182
                     /locus_tag="IVO76_000827"
     CDS             1421..2182
                     /locus_tag="IVO76_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7428810.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            2313..3647
                     /locus_tag="IVO76_000828"
     CDS             2313..3647
                     /locus_tag="IVO76_000828"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7428811.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            3954..4439
                     /locus_tag="IVO76_000829"
     CDS             3954..4439
                     /locus_tag="IVO76_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7428812.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            4432..5115
                     /locus_tag="IVO76_000830"
     CDS             4432..5115
                     /locus_tag="IVO76_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7428813.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            5241..7886
                     /gene="polA"
                     /locus_tag="IVO76_000831"
     CDS             5241..7886
                     /gene="polA"
                     /locus_tag="IVO76_000831"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7428814.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            7931..8767
                     /gene="mutM"
                     /locus_tag="IVO76_000832"
     CDS             7931..8767
                     /gene="mutM"
                     /locus_tag="IVO76_000832"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7428815.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            8731..9360
                     /locus_tag="IVO76_000833"
     CDS             8731..9360
                     /locus_tag="IVO76_000833"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7428816.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            9412..9744
                     /locus_tag="IVO76_000834"
     CDS             9412..9744
                     /locus_tag="IVO76_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7428817.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            10056..10547
                     /gene="nrdR"
                     /locus_tag="IVO76_000835"
     CDS             10056..10547
                     /gene="nrdR"
                     /locus_tag="IVO76_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7428818.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            10570..11943
                     /locus_tag="IVO76_000836"
     CDS             10570..11943
                     /locus_tag="IVO76_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7428819.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            11943..12869
                     /gene="dnaI"
                     /locus_tag="IVO76_000837"
     CDS             11943..12869
                     /gene="dnaI"
                     /locus_tag="IVO76_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7428820.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            12998..13438
                     /locus_tag="IVO76_000838"
     CDS             12998..13438
                     /locus_tag="IVO76_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7428821.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            13582..14283
                     /locus_tag="IVO76_000839"
     CDS             13582..14283
                     /locus_tag="IVO76_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7428822.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            14280..15401
                     /locus_tag="IVO76_000840"
     CDS             14280..15401
                     /locus_tag="IVO76_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7428823.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            15416..16648
                     /gene="pepT"
                     /locus_tag="IVO76_000841"
     CDS             15416..16648
                     /gene="pepT"
                     /locus_tag="IVO76_000841"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7428824.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            16780..17688
                     /locus_tag="IVO76_000842"
     CDS             16780..17688
                     /locus_tag="IVO76_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7428825.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            17723..17995
                     /locus_tag="IVO76_000843"
     CDS             17723..17995
                     /locus_tag="IVO76_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7428826.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            18006..19052
                     /locus_tag="IVO76_000844"
     CDS             18006..19052
                     /locus_tag="IVO76_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7428827.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            19303..21912
                     /gene="clpB"
                     /locus_tag="IVO76_000845"
     CDS             19303..21912
                     /gene="clpB"
                     /locus_tag="IVO76_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7428828.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(22063..22734)
                     /locus_tag="IVO76_000846"
     CDS             complement(22063..22734)
                     /locus_tag="IVO76_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428829.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(22727..23221)
                     /locus_tag="IVO76_000847"
     CDS             complement(22727..23221)
                     /locus_tag="IVO76_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7428830.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(23241..24461)
                     /locus_tag="IVO76_000848"
     CDS             complement(23241..24461)
                     /locus_tag="IVO76_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7428831.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            24608..26443
                     /gene="lepA"
                     /locus_tag="IVO76_000849"
     CDS             24608..26443
                     /gene="lepA"
                     /locus_tag="IVO76_000849"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7428832.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(26537..27619)
                     /locus_tag="IVO76_000850"
     CDS             complement(26537..27619)
                     /locus_tag="IVO76_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7428833.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         9196 a         4964 c         5885 g         7578 t
ORIGIN      
        1 gcacgaaaag aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt
       61 cgcctgcaag atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc
      121 cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa
      181 acagcggtta accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat
      241 atatcaggat tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga
      301 gaattcaccg cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa
      361 caagcaatcg gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc
      421 catcccgaaa gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa
      481 gagcttggta cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct
      541 gaaagcctat cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct
      601 ggacgagata tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg
      661 gaagacttga aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc
      721 gcatttgttg atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa
      781 aaattcgtca agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata
      841 gaacaagtcg atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag
      901 taacaatgag gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat
      961 atcattaaaa agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg
     1021 tacaactggc gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt
     1081 tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca
     1141 tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa
     1201 aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta
     1261 ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa
     1321 atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt
     1381 taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag
     1441 gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt
     1501 tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga
     1561 taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt
     1621 ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga
     1681 tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga
     1741 taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact
     1801 ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac
     1861 actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt
     1921 cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac
     1981 agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga
     2041 aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc
     2101 tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa
     2161 aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt
     2221 ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa
     2281 gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt
     2341 ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc
     2401 taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag
     2461 caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg
     2521 caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg
     2581 aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg
     2641 taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg
     2701 tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat
     2761 tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg
     2821 caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat
     2881 ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg
     2941 atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa
     3001 acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct
     3061 atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct
     3121 taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg
     3181 cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag
     3241 acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg
     3301 gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta
     3361 cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt
     3421 acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc
     3481 ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt
     3541 tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac
     3601 aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca
     3661 gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt
     3721 gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc
     3781 aaaaacatga gaagctgttt tttcttggct gttcttatta tttgatgcag gacagctttt
     3841 tcacgacctc tgtaaactat aggaatctgt taaatcaagg gagcagaact tgccttttac
     3901 gctacttttg gtacaataat gtatgattaa aaataaactg gagggtttct atcttgaaca
     3961 taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat cgaggaaggc gactcgttat
     4021 ccttaacaga atctattgaa gataaccagc ttttgctgta tttgggattg acgaatgatg
     4081 caaatccttt gtatatccaa catgactttg cacaaaaaac agaatatcaa cagccaatcg
     4141 ttccatcagt gatgctgatg gggatcatca caagtgcaat ttcaaagcat cttcctggac
     4201 caggatcaca tgttgtcaat ttttctgtca attttattga accggttttc cattatgaaa
     4261 cactgacttt tcagtttgag gtcataaaag tagataagat gaaggatgtg gtgacgattt
     4321 ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga tgctgtcgtt atggtccaac
     4381 cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga aggtgaattg aatgaataat
     4441 ccaagtacat caatgtcagg tatcaatcgt ttttatgcaa aagtttatgg atttttaggt
     4501 atgggacttg cccttagtgc agtcacttct tacttggctc tttgggtatt tcctttgcaa
     4561 ttagcaactt ttatcaataa ttttccacta ggctttgtgg gtctgtggat cgttgaactg
     4621 attttagtaa tggttctagg cgtaaaagca caaaagaatc cgagcttagc aatcggtgga
     4681 tttattgcct actctctttt gaatggcata acattagcgg ttactcttgc atactacgat
     4741 attggaacag taacgcaagc tttcttgaca gctactttga tgttcatggg gatgtctgtt
     4801 gtcggagtaa caacaaaaag agatcttagc ggggttggaa gagcaggaca ggcagcttta
     4861 atcggagtga tcattgcact agtcttgaac atcttcattt tgagaagtag cgtagttgag
     4921 ctgttcatct ccctactgtt agtcgtcatc tttgcaggga ttacagctta tgataaccaa
     4981 agcatccgta atatctatgt ccagacaaac ggaacagcac atagtggaat tgctgtattc
     5041 atggcattga agctgtattt ggactttatc aacttgttct tggcgtttct gagaatcttt
     5101 ggacggaaca actaaaaaag aaaaaaattc atcagttggg gctataccga attatttggt
     5161 atggtcccgc tttttctttt cgcattccgc aggattcttt gttagaatga ataagaactt
     5221 tacagtaagg agttaagaag atgacaaaaa ataaattatt actcgttgat gggaacagtg
     5281 tggcttttcg cgcattcttt gcgcttcaca attctcttga acgtttcaaa aatagaaacg
     5341 gtcttcatac aaatgcaatc tatgcattca atacgatgtt tgaaaatgtt atgcaaaaag
     5401 agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa aacgactttt cggacagaaa
     5461 tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg cgaattcaaa gaacaaatgc
     5521 catatatacg tgatttgttg acaggactag gggtacagta ttacgaattg cccaattatg
     5581 aagcagatga tattatcggt actttggcag aaaaagtaga caaagatcaa tttgatgtcg
     5641 tcgtgctttc aggtgatcgg gatttgactc aattagctag taaagaagta aaagtggata
     5701 tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc agaacatgta gcggaaaaat
     5761 atgatgggtt gacgccaaaa cagattattg acatgaaggg actagccgga gatgcctctg
     5821 acaatatccc cggtgtgact aaaatcggtg aaaaaacagc tatcaagcta ttgaaacaat
     5881 atggctcagt cgaaggaatt tatgaacata tcgatgaaat gaagcaaagc aaaatgaaag
     5941 aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa aaaattggcg accatcaata
     6001 cttcagcgcc tgtggatgtc tctatcgact cattgaagta tgaagggaaa aatctcgata
     6061 aactcgttcc gttttataaa gaaatggatt tcaatcagtt cctttctaaa ttgaatatcg
     6121 tagaagaaga agtaaaaatg gatgatattt tgtttgaagt cgttcatgaa ttcaaagagg
     6181 aaatgtttac tacggatatg gcgttgtacg tggaaatgat gggcgacaat tatcatacag
     6241 aggaaatcgt cggagtagcg tgggggacag aaaagaaaat ttatgtcacg aacgatctat
     6301 ctgtattcga aaaccgagcg ttccattctt ggatcactga tccgactcgg ctgaaaaaag
     6361 tatatgatgc gaaacgaact tatgttgctt taaaccgcta tactggaaaa acgaaaggaa
     6421 tcgattttga tgtcttgcta ggtgcatacc ttctcgatac gaatgaaaaa agcacagata
     6481 tcgaaggaat agctgctcat tacggataca acgatatcca atcagatgaa gctgtatacg
     6541 gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact cttttttgct catttagcgc
     6601 gaaaagtagc tgcaatcaat gcgttgacac caaaattgag ccaagaactg gcagaaaaaa
     6661 accaagaaga tcttttccgg aaaatggaac tgccattgtc tatcattttg gcagagatgg
     6721 aaataagcgg gatcaaagta gatgcgactc gcttacaaga aatgaagggc gagttctctg
     6781 ctcgcttaag agagatcgaa caaaaaatct acgaagaagc cggcgaagaa ttcaatttga
     6841 attcaccaaa gcaattagga gtgatcttat ttgaaaaaat gggattaccc gttatcaaaa
     6901 aaacaaaaac tggatattcc acagccgtag atgtactgga acagcttcgt gaacaagcac
     6961 caatcgtaga ggatatcctc acctatcgtc aaattgcaaa aattcaatct acctatgtcg
     7021 agggactgct gaaagtgatc caatcagatg gaaaagtgca tacccgttat gtccagacat
     7081 tgacccaaac aggacgatta agctctgttg atcctaattt gcagaacatc ccgattcgat
     7141 tagatgaagg gagaaagata cgtcaggcat ttgttcctag agaaaaagac tggctgattt
     7201 attcttctga ctattcacaa atcgagttac gcgtacttgc gcatatctca aatgatgaac
     7261 atttgaaagc tgccttccta gaaggtcaag atatccatgc cagcacagcg atgcgtgtat
     7321 ttggaatcga aaaggcagag gatgtcacac caaatatgcg taggcaggca aaagcagtga
     7381 attttggtat cgtgtatggc atctctgatt acggactttc acaaaatcta ggaatcagcc
     7441 gaaaagcagc acagcaatac atcgatacgt actttgaaaa atacccaggt gtcaaagaat
     7501 atatggaaag aatcgtacgc gaagcaaaag accaaggcta cgtcgaaacg ctgtatcacc
     7561 gccgtcgata tctgccagac atcaattcaa gaaattacaa tatccgttct tttgcggaac
     7621 gaacagctat caatacacca atccaaggaa gtgctgctga tattttgaaa atcgcgatga
     7681 tcgaattgga taaacgatta aaagaaacag gtcttcaagc tacgatgctg ttacaagttc
     7741 atgatgaact tgtcttcgag gtaccggaaa aagaacttga atcattagat aaattggtga
     7801 aggaagttat ggaacaagct gtttctcttc atgtaccact gatcactgac agcagttggg
     7861 gaaaaacgtg gtacgaagca aaataaacat tgtttaagac agagcaatat aatgaaggtg
     7921 aaaagaaaaa atgccagaat taccagaagt agaaaccgtt cgtaaaggat tggaacggtt
     7981 agtagtagga aaaacgattc aaaaagttca ggtcttgtgg cccaagatca tcgagcaacc
     8041 agagacaccc atctttgagg cttctcttgt tggcgaaacc atccaatcca ttggacgtag
     8101 agggaagttt ttaatttttc atctcgatca ttgtgaactg atctcacact tgcgaatgga
     8161 aggaaaatat caatttacga aagaaaatac accaatcgat aaacatacgc atgtactttt
     8221 cttttttgag gatggaagcc aacttcgcta caacgatgta cgaaaatttg gtcgtatgac
     8281 aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc atgaaattgg gacctgagcc
     8341 tttgccagat agcttcttat tagcagattt cgcaaatgga ctgaaaaagt cccacaaagc
     8401 aatcaagcca ttgctgctag accaacgtct agtgactggt ctaggtaata tttatgtaga
     8461 tgaagcatta tgggaagcga agatccatcc agaacaaccg gcgaacacac tgcgccccaa
     8521 agaggtagaa caattacgtc tatcgatcat tgatgttttg gatcgtgcaa tagaagccgg
     8581 aggtaccacg attcgcagtt atttgaatgc attaggagaa tcaggcggat ttcaagtagc
     8641 actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt tgtggaacgc cgatcgtgaa
     8701 aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat tgtcaaaggc taaaaatcgc
     8761 aaagtaggat ttgttttagg tttgacgggt ggaatagcta ccggtaaaag tacggcagcc
     8821 aaagtctttc agtctcatgg atttcctttg atcgatggcg atgtgattgc cagagaaact
     8881 gttgagcctg gaacgcttgc tctgaggaaa attgtatcag tgtttggcca agaggtattg
     8941 cagcctgatg ggcaactaga tcgagggaaa cttgggatga ttgtttttcc atcaaaggaa
     9001 aaacggcaaa aacttgatca gctattagat ccgtttatcc gaaaagcgat aaaagatcaa
     9061 atcgcctctc taagttccaa tcatccttta gtaattgtag atatcccatt gctctacgaa
     9121 ggtcattatg accactatat ggatgcagta gctgtcgttt atactacgcc agaaactcag
     9181 cttcagcgtt tgatgaaacg taatcaactg acgctagaac aagcgcagca gagaattagt
     9241 agtcaacttc caatcgaaga aaaaaagcag cgtgcggata ttttgttcga caacaatgga
     9301 acaaaagaaa aacttgtggg acaaatcgaa aattggcttt ttgctaatcg ctttctttga
     9361 gtatctgtgt caattttgct atcctgagta tatagaaagt gagggatagc gatgggatca
     9421 ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag aaaaaatcgg cgagatgctc
     9481 aataaagaaa atctaatcga tgaaggtcgt acgcaatcag aaacagcaga agaaaaagaa
     9541 aagcagtacc aagcaagaat cgatgaaata gaagaccagc aggcagaaga agaagcgaag
     9601 cggcaagatt tgactcaaga acgaaaaaat ccgacacctg atcgtagtaa aacaccgctc
     9661 ccagaaagtg accacattcc cgatgagcaa aaaatcgagg aacagcgaat gaaacactat
     9721 acaggaatcg aagagaagtt ataaaatagt aaaaaatcga gatgtgactt gaagcagatg
     9781 agaaggtgtt catctgcttc aagttttttg tgctagatga ctaaatcagc ttttgtgtaa
     9841 gttatttctc agtaatcaag gtgatcagta ttggaaaatt gaaatttagt aaggacaggg
     9901 gacacaatta cgcatgaaag tagaaaaaat tgttcttata tccgtgtttt ttgggtagga
     9961 cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta gttgtaaaga aagacacatc
    10021 ctttatataa aatgcgatgc aagaggtgag acaaaatgag atgtcccaga tgtcaacata
    10081 ataattcacg agtaatcgac agccgtcaag cagatgatgg acgtgcgatc cgtcgccgtc
    10141 gcgaatgtga gaattgttcg ttccgattca ctacatttga acgaattgaa gctgctccgt
    10201 tattagtaat caagaaaaac ggggaacgcg aagagttcaa tcgcgataaa attttaagag
    10261 gactcatccg ttcagcagaa aaaagacctg tagctatgga acaaatggaa caaatcgtca
    10321 accatgtcga aaaccgcgta cgcagtctcg gagaaaacga agtatcgacg aatctaattg
    10381 gtgaatatgt gatggaagat ttagttgatt tagacgaaat cgcttatatt cgttttgcaa
    10441 gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa agagttgcaa gatatcgtgg
    10501 ataaagcaaa agaaaataat aaagagaata ataaagaaaa caattaagaa acaaaaagga
    10561 ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa aaaatattta ccaaggaaga
    10621 aaagcggctc cgctgagaga agacagcaag aacgccttgc tgtatcttta tcagccacta
    10681 atcggcggag aagccttgag tatctatttc accctgttat cagaaatctc tcaagaatct
    10741 ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta gtctaggctg cggtatcccg
    10801 caattttatg atgcaagaaa aaagctagaa ggaatcggct tactggatgt ttattttaaa
    10861 gaagatagtg atctgggacc tcgttttatt tatgagctgt tagaaccact cattcctaaa
    10921 gcatttttca aagactctgt tctggctttt cttcttctgg aaaaagttgg tgaacgacga
    10981 ttccagaagc ttacccaatt ctttgaacct aaaaggcata cagcagaggg atatcaaaaa
    11041 atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag cagtgtacgc tgctaatcaa
    11101 gaaaacatcg aatcgctgaa taaatctttt gaagaatctg aatcagtagc attacttgaa
    11161 gcagattcga cattcgattg gcagtttttg aatgattggc tggcaaaaaa acatattgca
    11221 agtcttgatg atccaatcat caaacaagtc gacatgtatc atcagcttta cggtttcgat
    11281 gaattgacac ttggcgaact gatcagtcaa tcttatgact ttagtgaaca aaaagtatct
    11341 gaaaaagaac tgcaaaaagc cattttggca atgcagagac gtccggtgaa gcaaacaaaa
    11401 gaaacagttg cggtttctga aaacagtaat gaaccctcgt caaacgaaca tttgacaaac
    11461 gcagcgcttc agctgataaa agaagcacag agcattccac cgatgaaata tctggaagca
    11521 atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa actggttact gcaagatctt
    11581 gtttctcaat caggtctttc cagtagcgtg atcaatgttt tgctgaatta tgtactagtg
    11641 atcaagaata atgctagctt aaatgccagt tatgtaaata cagtggcaaa tgaatgggca
    11701 caaaagaaaa tcgccacagc agaagaagcg atccagcaca ttcggcaaat aagtaatcaa
    11761 gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa ctggcaagcg aaatgttcga
    11821 cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg aaaaacagat cagcagcgag
    11881 aaaaaagcag agattgaccg ccgttttaaa gaatacctgg caaaaaagga gggtgatatc
    11941 tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag caaacgcaat tatcaagaaa
    12001 agtatgctga aatgatcgct gaagtcttaa atgatgcgga tgttcaagct tttctaagcg
    12061 cacaccaaga agaattgaca caagcggata ttgaaaaaag ttacgctaaa ttgtatgaat
    12121 tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac gatgatcgct cccggctatg
    12181 agccgaagct gacgttgaat tttcattatg tggatgtcac ttatgtacca acggaagcat
    12241 tgttagcacg gcaaagacaa gaagaaatca gaagccgagt aaaagcaatg gatatgccaa
    12301 aagatattca atcggcttct tttgaaaact ttgaacgtac agatggacga ggatatgcga
    12361 gcttagaagc cctagatttt gtagagaaat atactgctga tccaaaaggg tatcataaag
    12421 gactgtatct agtaggaagt tttggtattg gaaaaacata tttattagga gcaacagcga
    12481 gagaattagc gattgctgga ttcaccacga cgctagtcca ttttccgaca tttgccgttg
    12541 agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa gttagatgca gtgaaacgtt
    12601 cacctatttt gatgatcgat gacattggtg cagatgcgat gagtagttgg atacgagacg
    12661 aagtattcgg tgttatcctt caatatcgga tgcaggaaca gttaccaacc ttcttctcat
    12721 cgaattttac aatggatgaa ttggaacagc atttgtccgt cactcaacgt ggagatgaag
    12781 agccgctaaa agcgaaacgg ataatggaac gcatacgtta tttgacaaaa gaaatcgaaa
    12841 tgactggtcg caaccgacgc aatggttgaa cagttgacaa gtccaactga catttgacct
    12901 agtagtaaaa gcaggtcagc gaatcattat atggacagaa atgaccggag acaatcaccg
    12961 caacaagtaa tgcagaaact taaacggagg attttttatg agggctattt tttctagtat
    13021 atctgtagtt ggtctatttt ttcttttagc aggctgcggt agcgcagata gcaacacagg
    13081 ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca agcagtacga aaacggagag
    13141 acaaagcata tttgtcggaa cgattgaaag tatcactcaa gtagaagcag aagaagaaac
    13201 gcttcagttg aaactcgtcg atgtcatggc caaagaagat ccagcgaata tgggaacgtc
    13261 attcagcaat gacggagtga tcttaaatgc tactgctgaa aaaataagcg tggacataaa
    13321 tgaattgaaa atcggtgaca aagtccaatt tacattagtt gagatgccga tcatgacgat
    13381 gtctatccca ccgcaagttc ctggagattc gattattgat gtttcattga acaaataacc
    13441 aataacattt tttcaaaaat aagaaaatgt tgttttatag aacgaaaagg tatatttttt
    13501 aaggagatat gggaattttt ttattcctag tctccttatt ttatttggaa aatgctataa
    13561 ttatctagaa ggagtgaagc tatgaaccat ttagatttat ctcagcgctt agccgcagtg
    13621 ggtgactttg tccctgaatc cgctcgttta gcagacattg gatcagatca cgcttactta
    13681 cccgttgcat tgatattaag aggaaaaatc gaatatgcag ttgctggtga agtcgttcaa
    13741 ggtccttatg aatcagcacg taagcaagtc aaaaaagatg gattagaaga ccgtatcacc
    13801 gtacgccttg caaatggtct agatgctatc cagccacaag atggtattac agctgtcacg
    13861 attgcgggta tgggaggttc actgatccga gatatcctag aagctggcaa gttgaatcaa
    13921 cgcttaagcg gacaagaacg tttgatcctt cagccaaaca tcggagagcg tacattacga
    13981 tcttggctgc aagaaaatca gtataaaatc caatcagaaa aaatacttga agaaaataaa
    14041 aaaatatatg aaatcattgt agccgaaaaa gaacaaaaac cagttactta caatgaaatg
    14101 gagctgatgt tcgggccatt tctgcttgaa gagaaaagtg agatcttcaa atcaaaatgg
    14161 acaagagagc ttcaacaaag aaaagctatc cgtgaacagc tcaaacaagc agctaatcaa
    14221 gaggcacgtc aaaaacaaat cgagcaggaa atccaaatga ttgaggaggt tttaaagcca
    14281 tgacaatcag cggacgcact ttcatccaaa agtttgaaag ctattgtcct cagtggctag
    14341 ctgaagaagg agacccagtc ggactgcata tcggaacatt ggataagcca atccaacgag
    14401 taatgatgac attagacgtt cgtccagaag tggtcgaaga agcaatcaag aaaaaaatcg
    14461 atttgctgat tgctaaacat cctccaatct ttcgtcctgt aaaacggctg gttactgatc
    14521 agccacaaga aaaaatgtat gcagatttgt tgaaacatga tattgccgtc tatgcagcac
    14581 acacgaacat ggatattatt tgggatggac tgaatgactg gttttgtgaa cttctcggta
    14641 ttgaagtgga gagttattta gtaaagactc atgagattca ttataaaaaa ctggccgttt
    14701 atgtacctgt ggatcatgca cagaaaatgc gtgaagtatt agctgcagct ggtgctggta
    14761 cacaaggtga ttataccggt acaagtttta cctcgattgg acacggtcga tttactccgg
    14821 aagctggtgc acaaccagcg attgggaaag tagggaagac agaacaagtt caagaggcaa
    14881 aagttgaggt tattttgccg gaaactatcg aaaaacaagt gattcaagcc atgcggtcag
    14941 ctcatcctta tgaggaacct gcttatgatc ttttcgctat agatgaacca gtcgaaatgt
    15001 ttgggttagg acgagtaggt gaattgccgc aagaaatttc tatagaggca tttgtagaac
    15061 aagtaaaaga agcgttccaa ctagatggac tgagaatagt ccagccaaaa aatgcaaaat
    15121 cctctgtgaa gcgcattgcg atttgcggtg gaagcggtga aaaattttat ccgcaagcaa
    15181 tcgcccaaag agcagatgtc tatatcacgg gagatatcta ttatcatacg gctcatgata
    15241 tgcaaagcgc tgggttgatt gcaattgatc ccggtcatta tattgaatcg ttatgcaagc
    15301 aacgatttat cgagaaattt gaatcttgga aacaagaaga aaattgggat attaactttt
    15361 tcgtttctga aacaaataca aacccatttc aatttaatta aaaggagaga atactatgta
    15421 cgaaaatctt ttgccacgtt ttttacgata tgtcaaaaca gaaacacgct ccgatgcaac
    15481 aagcaaaacg actccatcga ctcagacaca agttgctttt gcgcaagaat tgaaaaaaga
    15541 attagaagag ttaggcttag ctgatgtttt ctataatgaa gataacggat tcgttatggg
    15601 aacattacca agcaatgttg atcatgaagt gcgttccatt ggatttattt cccacatgga
    15661 tacagcagat tttaatgcag taggagtcaa tccacaaatc attgaaaact atgatggcga
    15721 gtctacgatc gtactagatg cggaagaacg ctatacattg aatacaaaag attttccaaa
    15781 tttgaaaaat tatgaaggcc acacattgat cacaacagat ggcagtacat tactgggagc
    15841 agatgacaaa tcagggattg cggaaatcat gactgcaatg gagatcttga tcaataatcc
    15901 ttctatccct cacggtacca tcaaagtggc ttttggtcct gatgaagaaa tcggtgtcgg
    15961 agcggataaa ttcgatgttg agcaattcga tgtagatttt gcttatacag tcgatggtgg
    16021 tcctttaggt gagttgcagt atgaaacgtt tagtgctgct caagcaaata ttacgatcca
    16081 agggaaaaat gtacatccag ggacggcaaa agatacaatg atcaatgctc tacagttagc
    16141 aatcgatttt cataatcagc ttccagccga tgaagttcca gaaaaaacgg aaggatacga
    16201 aggtttcttc catttgatgg cattggatgg gaatcctgaa gaagcttcta tgagttatat
    16261 cattcgcgat catgatcgtg aaaaattcga agcacgcaaa gcacaaatca cacatatcca
    16321 agaagagttg aatcagcgat ttgatcaaga acgaatcaaa gtagatatgt atgatcaata
    16381 ttacaacatg aaagaaatca ttgaaaaaga tatgagcatc gtggaattag cgaaacaagc
    16441 aatgatcgag cttggtatct ctccagtgat cgaaccagtt cgtggaggaa cagatggttc
    16501 aaaaatctct tacatgggta ttccgacgcc aaatctattt gccggaggag aaaatatgca
    16561 cggacgattc gaatttgttt ctttacaagt aatggaaaaa gcaacagacg tgattgtaaa
    16621 aattgcagaa ttgaacacaa agagttaaaa tcgaaaaaga acagcttatg acaaggcgtt
    16681 cgtcccaatg atggaatgtc atctgtctaa ggctaaaggg caggacaatg gaattggttg
    16741 tcccgccctc ttttttgaag agtaaatagg aggtaaagaa tggaaaagct gctagttttt
    16801 tataacaaaa gttcagggaa agatgagggt gagaaattag cacgctggtt tcaagattat
    16861 gtaaaagaaa agcagccaga attatatgta gaactagctg aaactggccc atcgattgat
    16921 gatgcggaat tagtcaagaa agcagaagcc atagaagctg atactttagt aattataggt
    16981 ggagacggca cgattcatca tatcgtccaa gcgttccaaa aaacattatc tcaataccat
    17041 gtcggattac ttccaggtgg aactgtcaac aaccttgcac gtgttcttga gattcccctc
    17101 aaaaaagaag aagctgctga cgtgatcctg gaaggcaatg tacggaaaat agactatggt
    17161 caagtcaatg atgaagtcat tatctctaca ctgacaatcg gaatcttagc agatacagca
    17221 gtttggatca gtcaagaaga aaaacagcgt tacggctcgt ggatctttat caagaagttt
    17281 tttaagttat tgctgaagaa gaaaaaatat catttggatt tagaaacaga cgaggagcat
    17341 tggaaaggaa aatcccaact gttgacagtc acgatgtcta actccgtggg cggttttaca
    17401 aattttgacg actcagcgac cctagatgat ggaaaatttc atgtaacgat cgtaccttcc
    17461 ttagatattt tccgatttat tttttatttg cctagaatca tgagaggcaa attctatacg
    17521 atccccggca ttacttattt cacagccaaa cgcatgaaaa tccaagcgca ggaaaaatct
    17581 gtgggcactc gaacggatgg agacacgaca gatgatcttc cagttgaatt atctgttgta
    17641 tcaaaaggat taacagttta ttcaccaaag aaagaagaaa aagcttagaa atagtagtta
    17701 acagaaagtt ggaagaagag taatggatgt attgttacac aatcctttgt ggttttacct
    17761 tttcattgtg aatttttact tattttgtct gatggggtat gataaacatc aggctagaac
    17821 tagcggctgg cgtgttcctg agaataattt gatatttgtc agcctgttag gcggtggttt
    17881 gggaggattg atctctcgaa aagttttcca tcataaaact cggaaaaaga agtttactat
    17941 aggattcgct gcaggtgtac ttgttgattt gattctcatt tattttttcc attaaggagc
    18001 aggagatgtc gtctaaagga acgaaaacta aaatcattct gaatctgctt ttgctgattg
    18061 tgatttttgc cattatttat tatttaatca atcaatcatt tgcagatatc tttgccgaat
    18121 tgctgtctac cagtgttgtt gttttggcag cggctgttat attagggaca gtttaccaaa
    18181 tcgctgaagg gcgctcaatc aaagaaattg ctgaaccttt cgcgccgaat tttacaacga
    18241 aagatgggtt cttgacctct agctatattg cgttttaccg aatcgtttcg tttggtacag
    18301 gaacgctgat ctcggagatt tatttttata acaaaaaagg aatgaagcta tcccagggag
    18361 caggggtgac agcactccat atgattatgt acaaactggc agtgatcact tatgcattag
    18421 ctggcttggt catccaattc tctctttttt attctaatgg tccatcaatg atctggttta
    18481 tcatcgctgg tatccttttg acgatcgtga ttgttgcctt tttattggcg atgtccgtca
    18541 gcttgaatgt tcaaatattt tttgtcagct tatcgaataa gttatttaaa agtgagcgtt
    18601 tgcggaaatg ggtggataca tgcaatcagc agatctactc tctaagggaa acggtccaaa
    18661 ccattatcca agacaggact gcgctattgc ggatttatct atggaatatg ttaaaacttg
    18721 tattttggta tgtacttcct tatttgattc tgattgaaaa ccatcctaaa atagattttc
    18781 tactgaccat ttcgtttatc agctttgcag tagtgctttc aggggttatt cctacgcctg
    18841 ctggtattgg ttcctttgaa tttgtctatt tgttattatt cagaccagtt gttggaacag
    18901 ttgacgcagt ttcttcgctt ttactgtatc gtttcagttc ctttatcttg ccatttctct
    18961 atggatttgt gtatgtgatt gctgaacggc gaagagtgat caaagaagaa ataatagaag
    19021 taaaaaaaga gaataaagaa aatcaagggt aggtctttta taagatctac cctttttatc
    19081 aaatcaaaga aaaacgttat ggataagata attttacgtt tgtttggctc tttttttata
    19141 tacatacaat caattttgtt tttttaagat ttatataagt cgagaaaaaa agtcaaaaaa
    19201 ctatgtgtat attttgacca aaattgacca aagcgatata atagtttttg taaagatcaa
    19261 agaaataagt gatcttacct ttaattagga gggtatacct gtatgaatat tgagaaaatg
    19321 actacgacgc ttcaagaagc gattgctgaa gctcaacaga ttgcggtcac tcgaaaacat
    19381 caagatatcg acattgccca tgtatggaaa atctttttac aatctaatca ttttggtcga
    19441 aatttctata cggatgccgg attggatgtc gaatcttttg aacatgaaat tgatcgtcta
    19501 ctggatgagt atccagttgt ttctggtgga aacgtccaat atggacaaaa cctcagccag
    19561 aatttctttc gattattgaa tgaagcagac caaatacgtg aaagcttcgg cgacgagttt
    19621 ctttcaacgg aagtcgttat tttagcgttg atgaaattaa aaaattatcc gttaactgtt
    19681 tatttgaata aaaacggatt atctgaaaaa gaattgcgta aaaacatcga agagatgaga
    19741 ggaggagaaa gagtgacctc acaaaaccaa gaagaacaat acaaagcatt agaaaaatac
    19801 ggcgttgatt tagtacagca agtcaaaagc ggaaaaatgg atccaatcat cggtcgtgat
    19861 gaagaaatcc gtgatgtcat ccgtattctt tcacgaaaaa caaaaaataa tcctgttctg
    19921 ataggagaac ccggtgtagg gaaaacggca atcgttgaag ggcttgctca acgtatcgta
    19981 cgcaaagatg tgcctgaaaa tttaaaagat aaaacgattt tttcattaga tatgggtgca
    20041 ttgatcgctg gagcaaaatt ccgtggtgaa tttgaagaaa gactaaaagc agtcttgaaa
    20101 gaagtcaaaa aaagtgatgg acgtatcctc ttgttcatcg atgaaatcca caatatcgta
    20161 ggggcaggaa aaacagaagg aagtatggat gccggaaatc tgttgaaacc aatgcttgct
    20221 cgcggtgaat tacatttgat cggtgccaca actttagatg agtaccgtca atatatggaa
    20281 aaagacaaag cgttggaacg tcgctttcaa aaggtattag tcaaagaacc aacagtggaa
    20341 gatacgatca gtattctgcg tggattaaaa gaacgttttg agatccacca tggtgttaac
    20401 atccatgaca acgcattagt agcagcagct actttatctg accgttatat tactgatcgg
    20461 ttcttaccgg acaaagcaat cgatttgatc gatgaagcca gtgcaacgat tcgggtagaa
    20521 atgaactcta tgccaacgga attagatcaa gttactagac gtttgatgca gttggaaatc
    20581 gaagaagctg cactgaaaaa agaatcagat gatgcaagta aaaaacgctt gaaaaatttg
    20641 caagaagaat tagctgaact tcgtgaagaa gcaaatgcga tgaagatgca gtgggaaaca
    20701 gaaaaagaag aagtcaattc tgtttcagca aaacgtgctg aaatcgataa agcaaaacac
    20761 gaattagaag atgcagaaaa taactatgat ttagaacgcg ctgctgtatt gcgtcatggt
    20821 acgatacctc aattagaaaa agaattgaag gaattagaag ctaaagcaaa agacagcgag
    20881 attaagatgg ttcaagagtc cgttaccgaa aatgagattg ctcaagtcgt tggcagatta
    20941 acgggtatcc ccgtaacgaa acttgtagaa ggtgaacgag aaaaactaat taaattgaat
    21001 gaaacacttc ataaacgagt gattggtcaa gatgaagcag ttgatgcggt cagtgacgcg
    21061 gtaattcgtt cacgggctgg attgcaagac ccaaatcgtc ctctaggttc tttccttttc
    21121 ctaggaccaa ctggtgtcgg aaaaaccgaa ttagcgaaag cgttagctga gaacttgttt
    21181 gactcagaag atcatatggt acggatcgat atgagtgaat atatggaaaa acatgctgtg
    21241 tctcgtctag tcggagctcc tccaggctat gtaggttacg aagaaggtgg tcaattaacg
    21301 gaagctgtca gacgaaatcc ttatacaatc gtcttgttgg atgaaatcga aaaagcgcat
    21361 cccgatgtct tcaatatctt gttacaagtc ctagatgacg gacgcttgac tgattcaaaa
    21421 gggcgagtag tagatttcaa aaatacggtt ctgatcatga ccagcaatat cggttcacag
    21481 ttactgctgg aaggcgtgac tgctgatgga acgattccag aagcagtggc tgaacaagtc
    21541 aacacgctat tacgtggaaa cttcaaacca gaattcttga accgaatcga cgacacgatt
    21601 ttatttacac cattgagcct agacaacgtg aaaggaatcg ttgacaaaat ggtggcgcaa
    21661 ttggcacaac gtctggaaca tcaagaaatc ctgctgacaa tcagtgatga agccaaaacg
    21721 tggattgctg aaaatgctta tgaaccagca tatggtgcaa gaccattgaa acgatttatc
    21781 actaaggaag tagaaacacc actagctaaa gaaatcgttg caggacatgt tatgcctaaa
    21841 tcaaaagtaa cgattacttt acttgatgga caacttcatt tcaaaacaga agagctagaa
    21901 gaaatcgtct aatcttccat tgagactata aaagaaaaaa ggggtctgac acaactagtg
    21961 tcagacctcc ttttttcaaa taaacagtgt tccagaagta gtttcttcga aaaataagcc
    22021 gaaatttctt gaagctgccc gcttctgtca caacctcttt tattaggtat ttttcacttt
    22081 ttctaattta taactttcaa agtaacgctc atctttttta ctttgagacc atgcaaacaa
    22141 tcccattaag gcagcaccta tcgtatttgt cagcaagtcg cccatcgtat ccatcaatgc
    22201 tgctcgtccg acaaacagtg tgccatcaga agcagcaaaa cgttgaagat tcatgcctaa
    22261 gaactggtcg cataagaatt cccagaactc ccaaaacacg ccgcaaagtc cagcaaacgc
    22321 aaagccgaat aagaggaaca accaaggact ggtttttgat atctcagcat ctttcatcaa
    22381 gtaaccaatc aatccatagc caagagctgt caggaccatt ggactcaccg catgtaagat
    22441 tttgtcccaa aaagagatga tactgatcaa atgcatccct gtgcctaaaa agacagagat
    22501 gaacaagaaa aaccagtaaa acaaaacgat tgctgctggt attttaagtt tgaaaacttt
    22561 caaaatgact tgtggcatga aaatcaatgc aatccctaat aaagattcca cgataaatgt
    22621 gattccttga gaagtcgaat atttttgtgt gataaattca taaaaattat agcagacaac
    22681 gaatacacca aaagcaagta aagctatttg cacctttttg atttctttaa tcactgaaag
    22741 tccctccatc taaaaatctt tttacttctg aaagcaactc aatttctgaa gaaaccactt
    22801 ttccgttcaa tttgatcaaa cctattgtat ataagttcag gtagtgaaat tgattttcag
    22861 ctgtttcttg tagtgcagcg atttttttcg ggtgggttcc gccttgctgt cgagtatcag
    22921 tatataagcc gatcactggg atgcctttcg cataagccac accgatttcc gatgctaccc
    22981 cggcatcgat cgtcagccca tctagtaaag caatcattaa atcactagcc aacacttttt
    23041 ctgtgtcagc aagagcaatc attcgactgt ctgcataagc agttttgtca ttgattgctt
    23101 cattttcttg aggaagatag atcgaaagct cagaagagag ctctctgatt tctttaacta
    23161 actgttggtt atataacaaa tcacttttag caaacattgg tgcggcaaaa taaattttca
    23221 tctaaacatc atccttttta tcaaaaataa tcgctaaagt aagcttccat gtttggaatg
    23281 atacgctcta gtgacttcag tgtttcttca tctgtttcta accgttcgat tcttgccaaa
    23341 taagaagcac ggcgataatt catcatcaag caagtcaatg tttggatatc cagtcgaacg
    23401 gattttccta taggttcatt taagacacgg acttgtccct gttcatccca tgttaaagcg
    23461 aagacaccat tattccaagg agcgacagga tcttcaatga tgaaatgaaa agcatcagct
    23521 gttccaacaa acggaaaccg ttgaaggaat tcttttacat caacgatccg agccataaaa
    23581 tatggttcga tctgctcttt gatctgacta tcttcaatca aaaatgctaa aggctcattt
    23641 ttataaatat ctccatgtac ccagtagatc atggaaaaat gtgcggaaat aaagttccat
    23701 aagccatttc gagcttcttg atcaagatag aacatttctt ttacgtgaaa tatttcttca
    23761 gctacccagt agaacagaac acctcttggt tcatggttgc tatcgtagta gacagcggct
    23821 gtgcgctcat cttcattttc gaaacgccaa tactcttccc aatgaaaatc actgcggaat
    23881 aaagcaccat ggttttgttg ggcaaatttg tcataaacgg aaaatacgtc tggatgatca
    23941 actggtaatc gctcaaccat accacttact gggatttgtt taggtagctg tgtatctctg
    24001 attttaaagg aaagtttatc agacatgatt tcccatcctt tacgccgata gtaggggata
    24061 ctgtaaggat acaaataaga gatccattgc ttattcttgc gcatattatc caatgcaacg
    24121 ataattagat cttgcatcaa tccatggtta gcatattctg gataagttcc aactccagtc
    24181 acaccaccca ttttataacg tgcaccgtga atattgactt cacaaggata gatagcgatt
    24241 tgagagatca gtttattttc gtggaaccat ccgaacactt tcgatacttc caaaatcggt
    24301 tttttggaac ggataaaagc tcgtttattc tcaaacccgc tttcctcgat gtctgcttca
    24361 gtcacttgga acacatagga aagcaattca ttgaattgat cgatatgttc ttgagtgacc
    24421 ggttttaatt caagattttc tctaaaatct ttttgatcca ttttttcatc tccatttatt
    24481 agcttgataa cagtatagca aaattctaca aaaaaaactt ctatagctga ctaattaaaa
    24541 actctttaaa ttactgcttt tcgttggtat aatggaaagt gctgattata tgaaaagagg
    24601 agacgctatg gatataaaga aaatgaaaga acgccaagag cagattcgta atttttcgat
    24661 catcgctcat atcgaccatg ggaaatctac attggccgat cgtattttag aaatgacgca
    24721 cactgtgacg tctcgcgaaa tgcaagatca gctgttagac tcgatggatt tggaacgtga
    24781 acgtggaatc acgattaaat taaatgctgt agaactcaat tacacagcga aaaatggaga
    24841 aacatatatc tttcacttga ttgacacccc agggcacgtc gacttcacct atgaagtctc
    24901 tcgaagcctt gctgcctgtg agggtgccgt tttagtggtc gatgcagcac aaggaatcga
    24961 agcacaaaca ttggccaatg tttatttagc gttagacaat gatttggaga tcctgccagt
    25021 catcaataaa atcgacctac ccgcagcaga tcccgaacgt gtccgtcaag aaatcgaaga
    25081 cgtaatcgga atcgatgcta gtgaagctgt attagccagt gccaaatcag ggattgggat
    25141 tgaagatatt ctcgagcaga tcgtggaata tgttcctgca ccaagtggag acttagatgc
    25201 accgctaaaa gctttgatct ttgactctat ttatgatagt taccgcggtg ttgttctaaa
    25261 tgtccggatc acggacggta tggtcaaacc cggagacaaa atcaaactga tgagtaatgg
    25321 caaaacattt gatgttacag aggtcggggt cttctcgcca aaagcagttg ctagagactt
    25381 tttgatggtc ggtgatgtcg gttatatcac agcaagtatc aaaactgtcc aagatacacg
    25441 agtaggggat acagtaacgt tagcagacaa tcctgccgct gaagctttac caggttaccg
    25501 aaaaatgaat ccgatggttt attgtggtct ttatccaatt gatacttctc gttacaacga
    25561 tttgcgggaa gctttagaaa aactgcaatt gaatgatgct gctttacaat tcgaaccaga
    25621 aacctctcaa gcattaggtt ttgggttccg ctgtggattc ctcggacttc ttcatatgga
    25681 tgtcgtccaa gaacgcttgg aacgggaatt caatctagaa ttgatcacga ctgcaccatc
    25741 cgttatttac catgttaata aaacggatgg tacgactgct acagtagata acccggcaga
    25801 ttttcctgaa cctgtaacga ttcaggatgt agaagaacca ttcgtcaaag cacagatcat
    25861 ggtcccaaat gattttgtcg gtgcagtaat ggaactttct caacgaaaac ggggagaatt
    25921 cattacgatg gattatttag atgattaccg agtaaatgtc gtctataata ttccgctttc
    25981 tgaaattgtc tttgactttt tcgacaaatt gaaatcaagc acgaaaggct atgcctcatt
    26041 ggattatgaa atgtctggtt atcaaaaaag caagttagtg aagatggaca tcttgctgaa
    26101 tggagaaaaa gtcgatgcat taagctttat cgttcaccga gactttgcct atgaacgtgg
    26161 aaaagcaatc gttgaaaaac tgaaaaaact aatcccacgc caacaatttg aggtgccaat
    26221 ccaagcagct attggacaaa aaatcgttgc ccgttcagac atcaaagcct tgcgtaaaaa
    26281 cgtcttggct aaatgttatg gtggagatgt ttctcgtaaa cgtaaactct tagagaaaca
    26341 aaaagaaggg aagaagcgga tgaaacaaat cggctccgtc gaagttccgc aagaagcctt
    26401 catggccgtt ctgaaaatgg acgaggacga accgaagaaa tagcaagggt ttgtttgaaa
    26461 acagtgtaaa aaataaacca tgtttacatg gatttgccac cggtttgcca ccggtggttt
    26521 ttttcggtgg caaaaatcaa gaattctttc taagattttc catgatatcg accgtttcat
    26581 ttctcatctt atctgttaca tgcgagtaag tatccatcgt aattgaaatt ctgctgtggc
    26641 ccaatcgttc agagatttct ttcatttttg cgccattttc gagaagaagt gtagcatgag
    26701 tatgtctgag agaatggaag ttaaaagaga gggagagtgc attcgatatc ctcctagtat
    26761 tccatttcac tacacttggc gtaactagct caccgtcttc cttcgtacat actgcatttg
    26821 aatcaatgta gagctttcca tacttcattc gattttctaa ttgttgtttc ttatgttttt
    26881 tcagaattgc tagcaaggtt tgtccaataa aaatcgttcg attggaactg cttgtctttg
    26941 gcgtaccata tacccatgcg ccgtcattct ttaccatttg tttctctaca gtaattgttc
    27001 catttgaaaa atcgacatca tcccacgtca gaccacaaac ttcgccaacg cgcattcccg
    27061 tataaaatcc aatattcaaa ggaatataga aaggatgtcc ttcaggagta atttctagca
    27121 tatgatcaaa gtcctcaaga gaaatgattt ttagatcttt tttagtcgtt ggtcgtgctt
    27181 catattttgg tatttttaca tacagcatag gattttgctt gattaatccc caaggataaa
    27241 ccgccatatt cagcgcattc ttaaggacag agtgagtaat agtcattgtt ttcttcgagt
    27301 aacccttttt aaattcagca ttgatgaaat tttgtaaaag agcaggggag agatccgtaa
    27361 gttttttctt tcctaaataa ccgtttatat gatttttgat ggtaaatcgg tagttttcat
    27421 aggtattgta ttttagattt agtttaacgt attcctccat ccaaaaatca aggtattgtt
    27481 ttactcgagt atccgtacct aaaaagtatt gtcctgtttc gtcaatatct gataaaactt
    27541 ttcgtaaagc agcttcggcc tctggtcggg tgtctccgcc aactttctcc actttttttc
    27601 ttgagccatc atcattgata tct
//
LOCUS       DACCQI010000025        27585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCQI010000025 DACCQI010000000
VERSION     DACCQI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.025"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IVO76_000851"
     CDS             complement(287..649)
                     /locus_tag="IVO76_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428834.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IVO76_000852"
     CDS             complement(723..1103)
                     /locus_tag="IVO76_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7428835.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IVO76_000853"
     CDS             1185..2360
                     /locus_tag="IVO76_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7428836.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IVO76_000854"
     CDS             complement(2437..3021)
                     /locus_tag="IVO76_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7428837.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IVO76_000855"
     CDS             3222..4220
                     /locus_tag="IVO76_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7428838.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IVO76_000856"
     CDS             4332..4841
                     /locus_tag="IVO76_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7428839.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IVO76_000857"
     CDS             complement(4871..5530)
                     /locus_tag="IVO76_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7428840.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IVO76_000858"
     CDS             complement(5532..6296)
                     /locus_tag="IVO76_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7428841.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IVO76_000859"
     CDS             complement(6304..6981)
                     /locus_tag="IVO76_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7428842.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IVO76_000860"
     CDS             complement(7018..8403)
                     /locus_tag="IVO76_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7428843.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IVO76_000861"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVO76_000861"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVO76_000862"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IVO76_000862"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7428844.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IVO76_000863"
     CDS             9342..10199
                     /locus_tag="IVO76_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7428845.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IVO76_000864"
     CDS             complement(10280..10615)
                     /locus_tag="IVO76_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428846.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IVO76_000865"
     CDS             complement(10682..12052)
                     /locus_tag="IVO76_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7428847.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IVO76_000866"
     CDS             complement(12130..12666)
                     /locus_tag="IVO76_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7428848.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="IVO76_000867"
     CDS             12975..14456
                     /locus_tag="IVO76_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7428849.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(14559..15266)
                     /locus_tag="IVO76_000868"
     CDS             complement(14559..15266)
                     /locus_tag="IVO76_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7428850.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(15345..15683)
                     /locus_tag="IVO76_000869"
     CDS             complement(15345..15683)
                     /locus_tag="IVO76_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7428851.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(15794..16756)
                     /locus_tag="IVO76_000870"
     CDS             complement(15794..16756)
                     /locus_tag="IVO76_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7428852.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(16771..18102)
                     /locus_tag="IVO76_000871"
     CDS             complement(16771..18102)
                     /locus_tag="IVO76_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7428853.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(18110..18790)
                     /locus_tag="IVO76_000872"
     CDS             complement(18110..18790)
                     /locus_tag="IVO76_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7428854.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(19009..19488)
                     /locus_tag="IVO76_000873"
     CDS             complement(19009..19488)
                     /locus_tag="IVO76_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7428855.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(19615..21294)
                     /locus_tag="IVO76_000874"
     CDS             complement(19615..21294)
                     /locus_tag="IVO76_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7428856.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            21842..23344
                     /locus_tag="IVO76_000875"
     CDS             21842..23344
                     /locus_tag="IVO76_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428857.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            23357..24019
                     /locus_tag="IVO76_000876"
     CDS             23357..24019
                     /locus_tag="IVO76_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428858.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(24120..24323)
                     /locus_tag="IVO76_000877"
     CDS             complement(24120..24323)
                     /locus_tag="IVO76_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7428859.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IVO76_000878"
     CDS             complement(24434..25618)
                     /gene="iadA"
                     /locus_tag="IVO76_000878"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7428860.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="IVO76_000879"
     CDS             complement(25775..27514)
                     /gene="yfcC"
                     /locus_tag="IVO76_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7428861.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
BASE COUNT         8209 a         5761 c         4670 g         8945 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 actatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta ttccaaataa atggtattca atcagctaac tctcggtatt
    14521 agctcagatc aaagcgctga tttcacaact tctttatttt aatctcttcg gttgccgccg
    14581 aacaataaga tcaagcgcaa cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc
    14641 agtgctgctg caaataatac gtgtcttgcc atcggtactt cttcttccgt cagtagtccg
    14701 ccttcactca aaatttggat tgctcgccga gacgcgttaa attcgactgg taatgtcacc
    14761 aattggaaaa gtaatgctaa agaaaaagcg aaaatcccga tattgattaa cgtagagttc
    14821 cagctaagca atacacctac cataatcaag gggaacgaaa tcatcgaacc aaagttagct
    14881 gctggaacaa gtgctgcacg aatcttcaat gggacataac ccgaagcatc ttgtaccgca
    14941 tgtccgcatt catgagctgc tacacctatc gcagcaactg aagtagactg tgcagttgct
    15001 tgagacaaac tcaacatctt attacctgaa ttgtagttat ctgtgagatc tccggcaatt
    15061 tgttgaacac cgacattatt tatctgttga ctttgtaata tatattgtgc tgcttgtgta
    15121 ccagtcacat tgtttttgct tctcacttga tcgtattttc ggaaagtact attgacgtac
    15181 gctgacgcaa ttccggaaat aactaatcca agaatgacca aaagaaatgt cggatctaaa
    15241 aaaccataca ttccataacc atacattttc gtttcactcc ttttcgttta ctttatttta
    15301 tcattagtag taggaaaaga atgtgaaaag accttgaata ttctttaatt ctttttcaca
    15361 aattccgatt tcagtttcat cggtccaaat ccttctacct tgcaatcgat attatgatcg
    15421 ccttctacta aacggatgtt cttgactttt gttccttgtt taatggcacc tgacgcacct
    15481 tttactttca agtctttaat cactgtcacg ctatcgccct ctaacagacg gtttccattt
    15541 gcatctaata ccactaatcc gtcttctatg acagtttctt gttcttcttt gttccattcg
    15601 ttcccgcatt ctgggcaaat caacaagcct cgatcttcat atgtgtactc tgagccacat
    15661 tctgggcaat ttggtaatgt catcttcttt ctcctttaaa atctttagtg agcactattg
    15721 taacggaaaa agaagatctt gaaaagatac atttgtgatt tttactacaa aaggcacgtt
    15781 ttcgaacgaa gtatcattct gtttttctgc tcttatacga ttggcatata tcttggatct
    15841 ctttatagat ttcctctact tctgctaaat cttttgcatt cgcaccactt gctaatggat
    15901 ggcctcctcc atgatggcgt ttagccaatt cattgatgat cggcccctta gaacgtaaac
    15961 gtacgcggta ataccctgaa ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa
    16021 tcactccagg aagtgaaacg atcgaagctg tctctgaatc atcgattcca tatttatcaa
    16081 gaatttcttg cggaaggatt attcttcctg caccattttc atccacctgg atattttgat
    16141 aaagataacc ggaaagttta gcgactttca tcggcatttg ttcaatctct ctgttcaaag
    16201 cacttgcatc aaatggataa tttctcaata tcgaagcaat ctctaatgta cgggacgtcg
    16261 tggagggata caaaaagcgc cctgtatctc cgacgattcc tgcataaagt aaccgtgcag
    16321 ccttaacgtt catctttaaa gattcaggaa acatcaaagc aaattctgca atcatctcac
    16381 tacagctact tgcttttgta tttacccaca caagatctcc ataagggtca tcattgggat
    16441 gatggtcgat tttgatcagt ttttctgcca actgatatct ttgatcactg attctgggtg
    16501 aattagctgt atcagtcacg atgactagcg ctccttggta gaagacatct tcaacttctt
    16561 gcatttctgc taaaaagcga agcccttcaa tcgtttcacc tacttgacgt atttcttttt
    16621 ctgggaaact ttcccgtaat agttcggcta atcctacttg tgatcctaat gcatcaggat
    16681 cagggcgcaa atgcctgtgg ataatgattt tatcgtattt ttttattgta tctactattt
    16741 cttgctgtac tgtcatttca ctcctgcttt ctaagaacgt tccattactt gacaaacaac
    16801 gattgctttg gaaacgatgg tgttttcaat aaacacttcg atatccagtt tagcggaacg
    16861 tctgccgatt tccaatacac gtggacgaat gtctaattcg ctttccaatt ggatcagccg
    16921 caaataatgt aggttcactt gttcaatcaa gatatttcgt ttttggttca tcaccattgt
    16981 tttttgtgcg acatcagaaa tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc
    17041 attgaccatt tgtggtgcta ctacgaattt gaacttaggg ttttccaaac gatttccttc
    17101 acggtcagta tcaatcgtat ggatatttcc agatatctga tccgaaatcg tatctgcaat
    17161 ctgcggttgt cgctggacaa gctgcatcgc tttcattaca tcttgccggg taatcaagcc
    17221 ttccaatgtc aagtcatcgg aaacgaccgg catgacttct agcccatccc agatcatttg
    17281 atggctaaca gaggcaatac tcattgtttt ctttacactg attggttctt tcgtcatcac
    17341 ccgatcgatg atctggtttg gcgatttccc taaaacatct tttgccgtaa caatccccat
    17401 caaacgatga tggcggttga cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg
    17461 ctgataatca gaaatcgtat ctgtctgctg caaataatga gtcttttcag ctggcatata
    17521 gatatcactg acaagcatga tatctttttt gatcaattgg tcgcttaacg ctcggttgat
    17581 catggttgcg actgtaaatg tatcataaga ggtgcgtaag atcggcattt caagatcatc
    17641 agctagcttt gcaatctcag cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc
    17701 atcttccaat gctaattttt ggacttccgc ccggttgccg acgatcatta gtgatcctgg
    17761 cgtaatatag cgttccatgg catgtttagt catcgcgccg atgacgaatt tattcaagac
    17821 cttgtctaat cctgccgccc cgcctaaaac gtccccttca atgatcctga cgacctcgcc
    17881 aaatgtcaaa cgttcaatat gtttttttaa tttgcgttcg atacggatgg tccctactcg
    17941 ttgaattgtt gaaactagtc cgacattttc tgcatctttg attgcacggt acgctgttcc
    18001 ttcactgaca ttcaattctt ttgcgatgct tcttacagaa attcgattgc caacaggtaa
    18061 actttcaata tattctaaaa tcaaatcatg ttttgtcgcc attttctgcc tacacttcca
    18121 gcgtttccga gacgctcatt atttttcctt cactatcttc cagtaactct acaaaagatt
    18181 cgggatcttg ttcaatgaca gggaaagtat tgtaatggat tggaactact ttcttggcac
    18241 gaattgtttg cgcagccttt gctgcatctt ctggccccat cgtatagtta tcgccaatcg
    18301 gcaagaaagc gacatcaata tcgaattctt ctgcaagcaa tcctagatca ctgaaataag
    18361 cagtatcccc tgcatgataa atcgtttttc cttctgcttg aataataatt ccgctaggct
    18421 cgcccatata ctgaacgata ccgtctactt catagcttga actatgctgt gcatgtacga
    18481 attttaccac gccgaatgga aacgcatgtt ttccaccgat attcataccg aatgaatcta
    18541 ctcctctgct gttcgcataa tcagcaatct caacaatact gataattgtg gcttgatttt
    18601 cttttgcaat aggaatcata tctcctatgt ggtcactatg accatgagta atcagcaacc
    18661 aatctgtctt tacttcttct gcagttaagt ctgtcaatgg atttcctgta ataaacgggt
    18721 caatcatcac gcgttggcca ttttccattt caattgttaa acaggaatgt ccatgatacg
    18781 taattttcat attttacacc tcgcgttttc tctgtatctt ttttattaaa aacgatttca
    18841 tcacttgcac atcaatgaaa aaatatagct gctaaatata ttttcattgg attgatagta
    18901 cagtatgtac aaagcaattg taacagataa tacaggatgc accaagtacc aagcagatat
    18961 aatctcaagt ttgaggtctc tgctctgctt agcttctctt ctcgtccctt aactaattaa
    19021 atcagcgtaa ataggatgga cgatcttttc taaatcggct acttttatcc gaaggcttct
    19081 cccgatttca ccgcttgaca caattacttc ttctttttct tttaaagaac tgtctgctaa
    19141 aaaacaataa tcaggatgtg cgagttttat tccaataggg gtgtttgctc cgtggggata
    19201 tcctgtatgc attaatacaa aatccattcc aggaaaacca actttacgat ctttcgtcac
    19261 ttgtctcgtt tttttgtaat ctaagtgatc gttcaaagga atcaatgcga tgatcacccc
    19321 agttttgttt cctttcacta cgagagtttt acatatcaaa gctggatcaa tccctctttt
    19381 ttgtagttct tctgtgaaag attcttctac cacatgacta aaatcaagtg gttgatagct
    19441 gatcttttgt tcttctaaat aacgttcgac ttttgtttga ctcatcattg attcactccc
    19501 tttattctat cgtttattat aaagcgaggg atgctgtatt caaaaattat ttgtttttcg
    19561 caaaaaaaga gcaagacgag cactctatag agaggttcat cttactcttt ttgtttattt
    19621 tttatttcga cgttgagtag cttccataat gactggcaag atcactgggc ggcgttttgt
    19681 ttgttcaaat aagtaacggc ctaattgatc tcggatgtct tgctttaatt tgctccactc
    19741 aaaatcattg ctttctaaat gtttctcaac gattgtttca atcatattgc tgctttcttt
    19801 catcagatca cggctagctt ttacgtaaac gaatccccgt gaagtaattt gaggttttga
    19861 aatgattttc ttttctcgcc gactgatagt gacaaccgct acgaaaatcc cgtcttctga
    19921 caagattttt cgatcacgta aaacaatgtt tccgatatca cctatgccta atccatcaat
    19981 cattacattt tctgcacttg tagttcctgc aacactcata cgcttgttct tatattccag
    20041 cacgtctccg cgtccagtaa tatagatatt tttgtatggc atccctactg catgagccaa
    20101 atctgcatga gcagctaatt cacgatactc tccttgaact ggcacaaaat atgttggttt
    20161 catcaaattg atcattaatt gcaagtcatt aggattggca tggccagaaa cgcgcatatt
    20221 ctcagagatc aacttcactg ttccaccagc tcgataaatg atatcttccg ttttcgccac
    20281 gatggtttcc atcgcgatgc ttggcgtagt agtgatataa accaaatctc cttcttcaat
    20341 cttgacatta cggtgtgtac cgtttgccat tttttgcaga gacttgatgg gttcacccat
    20401 tcggccagtt tctaaaatga tcagttcttc tggatcatag tttttcatgt ctttcaacgt
    20461 aacaagcaga tcttcacttg gtaattgaag tttttctaaa cgcatcgctg tgcgaataat
    20521 tcgttcgaaa tcttgaccag tcaagacaac ttttcgttgt gctttttctg ctgcatccaa
    20581 tacttgttgc actcgttgca aattacttgc tacacaagcg acgatgatac gcccttccca
    20641 atatttgatc gtatcaaata cttcatctgc tatttgggct tcagaagcta caggctgagg
    20701 attttctgca tttgccgatt cacttagtaa ggctaagact ccttctttgc cgatttccgc
    20761 caatcgtgca taatctgttt gatacattgg gatagccgtt tggtcaaatt taaaatctcc
    20821 cgtataaacg atactacctt cttttgtttt cagattgatc cccactgaat ctggtatcgt
    20881 atgagtggta cggaaaaagc tcactactgt ttcaccaaag tctatttctg tatgctcatc
    20941 taccacatgg aaatccttaa atcctttggc tgcttcatta cgactgacat tcaattttgc
    21001 taactcgatc gtcaactctg tcccgaatac tggtacagaa atttttgaca acaagtaagg
    21061 aagtgccccg atcgcatctg catgtccatg tgttaaaaag acaccagcta cacgatcaat
    21121 gttttcttct aaatacgtaa aatcagggat tacgacgtcg atccccaaca actcattttc
    21181 aggatatttt agcccacaat ctaaaataaa aatatcctcg cccacttctg cgacatacat
    21241 gttttttcca ttttcacgaa cgccgcctaa tgggataatt ttaatggtac tcacaaactt
    21301 cacctcttct tatgacatcc aagtttaaag atgtcgattt tcttctttat ctaaatatat
    21361 gagccattcc aatgaagcat atgcaaaatc actatttctc aacttatgga gaatcagcag
    21421 gaaaattacc agcacatccg cgttctgaca gttggatggg cattttattt tggatatact
    21481 tcattaacaa aatggatctt ttgcgaaacg ctatagccag aatacttata taagtattca
    21541 gctgttcccc attattatac aacaaataaa ctaaatcgct agtatcagca attgttagaa
    21601 caactatgca cgtaaaaatc cctctgtagc atctaggatt tcttttgcta ttcctctttc
    21661 atttcttctt cgtcggtttg aaacatactt ttttcatttt accacatttt ttgcaataac
    21721 tatagtgact ttttatatat acagaacctc atgttgatgg tgtgcaaaca gcataagatt
    21781 taagctagct ttaagatagg ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa
    21841 tatgaatttt atgaaacgtg catggaaaag tacaaaagta aaatggggac gttcattgtt
    21901 attgtttgcc gtgttcacag ccattcttat ttttgtgcta gctggcctca cgattcgaag
    21961 cgctgcagaa gaagcagcga atcaagcaca aaaagaagtt ggagcaacag tgactttaag
    22021 tgccaatcga gaagcttcat ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac
    22081 tgaagaacgt ccagatccag gcagtttttc tcttacacca gtttccttaa cggacgcgga
    22141 aaaaatagct gaattagata atgtaaagag ttattccttt gaaagttccg cctcagctat
    22201 ggcaaaagat ggaataactg ccatcactag ttctgattca agtgaagaca gttcagaaac
    22261 tactgatgca ggcggtgaac ctgctggagg aatgggcggg aatccgcaaa tgactcaagc
    22321 agactttcaa atcagtggtg tgtcagcaac tgcacaaaat agtaattttt ctgatggaac
    22381 agcaaaaatc acggatggtg aaggaataga tgacagcgac aaaggcacaa acaatgtagt
    22441 cattgattct actctagcag aaacaaatga cctggcagtc ggagatactt tcacaatcac
    22501 gagtactgaa gatgaagata ccacttatga aatgacgatc aaaggaattt atgaatccag
    22561 cgaaacaagc ggcagtatgg gtatgaaatt caacttcatg aatccgtcga atacacttta
    22621 cacttcttac acttttgcaa atgaactaaa tgggacatct gacgataaca cgatagattc
    22681 tgctatttac actctatctg atccagataa aatggatgag tttctcaaag aagctgagaa
    22741 gctgatcgac acagatacat ttagcttaca atcaaatgat tccatgtatc agtcaatgtt
    22801 ggaaccttta aacaacgtgg caagtttttc taaaaatgtt gttttactag tggctgtagc
    22861 cggtattatc atacttactc tgatcattat gattaccatt cgggagcgtc ggcatgagtt
    22921 aggcgtcttg ctatctttag gagaatccag aagtaaaatc attctccaat tgtttaccga
    22981 agtggctatt tgtatgattc ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc
    23041 gaatgccgtg ggacagcagc tacttgacca acagacagaa accacgacac aaaaccagca
    23101 agctcctggc ggtggacaaa tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg
    23161 tcaacaagga accacgaata atccatttgc tgtttctgat caagtcagcg aattggaaat
    23221 caatgtcaaa cctgcacaat tgggactatt agccggagta gcatttggaa tcagtctgtt
    23281 ttctgtcttt ttagcttcta tagggatatt aagattaaac ccaagaaaaa tcctattaaa
    23341 ctaaaggagg aatttgatgt tacggacaga acaattggga ttttggtatc aagatccgca
    23401 agatgcgctt tataaagatg tagaactgaa tttcgaagct ggaaaaatgt acgctatttt
    23461 aggagcaagt ggttctggaa aaacgacttt tctttcgctt atttctggac tagatgcacc
    23521 aaaagaagga aaggtatact atcaagatcg ttcactgaac aaaatcgggt taagaagtta
    23581 tcgtaaaaat gatgtctcga ttattttcca agcttacaac ttgcttcctt atttatcggc
    23641 tctagacaac gtcatagcag caatggaaat cactaagacc aagcaagaga attatcgaca
    23701 agttgcttta gatcgactgg ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt
    23761 gaccaaactt tcgggaggtc agcagcagcg agtcgctatc gttcgtgcta tctgctgtga
    23821 acatcaacta atcgtagcgg atgagcctac tggaaatcta gatgaaacaa cttcacaaga
    23881 tatcgtcaaa cttttccaag aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac
    23941 tcatgagatt gaagtagcaa aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt
    24001 tcaaaaaatc gaagtttaaa aaacaggata atgatcaaat aaaaaatccg aaccatatct
    24061 gtctcgcaga tatggttcgg attttttatt tgcttgattc aattagtaat aagtggtttt
    24121 tatttttttt cgtcttttcg agaaaacttg tctttgactt cgtcgcctaa atttcgagct
    24181 ttttctttga tgtcagcacc tagtgattgt gctttccctt ctgtttcttt gctcttgtcg
    24241 tctgttgctt tgccgtatgc ttcttttcct tcaccgacta ctttatcttt catatcttta
    24301 gaacgtcctt ttaaatctgc cattgtgctt cctcctctta gtttgtttat tgatctacct
    24361 tcagggtaac atcaacaaca gatacagaca aacagaacgc tcgttttcta aaaaagatag
    24421 ttagagctgt tgattaaaaa cttttttcga aagtccccca tactttgact tctttttctt
    24481 ggaccattat ttctccttta gcgataactg tatcaatatc aagcgtttct ttatccaaga
    24541 tacacagatc tgcatcagca ccaatttcga tttttccttt tttatccaat ttcaatatac
    24601 gtgctggatt actggttata gctcgtaagg cgatttccaa agggatactt tctttttgca
    24661 cagcttcttt gatccctact agcaatgcct tggcacttcc tactcctaac cctaggaaat
    24721 gattgttttc atcaaaataa ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat
    24781 ctaaagaaac atcttcctcg atcaatcgtt tcagtgcttt gctgaagcga acttcaccat
    24841 ctgtcttctc ccaaaaatca ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt
    24901 tggcaaatgc cacgcaagct tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa
    24961 attgtgtgat cggaatttct gtttcttcta cgattcgtgt caatagctct agctttcttt
    25021 ttcctccgcc taagtggaca ttgatgatcc cagcttttcc ggataacatg cctcctacac
    25081 gagcatctgc tactgcacga gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg
    25141 atactgcgat ttctccgatt ccgataattt tatcaattgt caaaaagtct ttgatgatag
    25201 aatctgttac tgtttttacc ggcaatcggt acgaaccttc gtatacataa gtggagattc
    25261 cttctgcttc cagacctttc gctttgctga tcaacgcagt catatctcta ccttctccgt
    25321 ctgttcccaa acaaccgaca actgtcgtta caccagctgt agtcaattgg ctcaatgtga
    25381 cttctggcgt tcgattttga taaccgcctt ctcctcctcc acctaaaata tggaaatgac
    25441 agtcaatgaa acccggggtt agtacttttc cctcacctga aagttcttct acttccactc
    25501 cttcgtagcc gccttctagc tgttcttcta cagccaaaat cttcccaccg gcaatcagta
    25561 catccatttt tcctaagtaa tctggtgcaa acacttcaat ttgtcgaatg attttcatac
    25621 atttttcctc tttctttaag tataactgat gataggatct taaaaaataa tccaaacgat
    25681 aggatcttta ctcctttgat tttaccgtaa aatgacggtt cttggagtcc ttatctactg
    25741 tttggattac ttttttattt acaggtctcc agtactactg cagacctatt gcaatggcaa
    25801 tcatgatgaa aatagttcct aaaacaaaca ggaatccttg catcttgatt tggaatcttg
    25861 cccattttgc ccattcgatc cgtgcaacac ctagcacacc aatcaagctg gctgatacgg
    25921 gtgtaaaagc gtccataaaa cctgaaccaa gctgataagc aagaactgct acttgtctgg
    25981 atacacctac taaatctgcc agtggagcca tgattggcat agtcaaagct gcttgtcctg
    26041 aatttgatgt aacaaccaag ttgaacaaac tttggaagat gtacatcgcc caagcaccaa
    26101 tgacagctgg aacaccggtc aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg
    26161 aaggaacaga tgcatcagat cctcccaaga caagaagaat ccctttggcc atacctacta
    26221 cgattgctgt accagctaaa tcagctgcac cagattggaa agaagaagcg atgtcattga
    26281 ctttcattcc atctaatttg aaaagaacag caatgattcc agcaactaat cccatcacaa
    26341 agaattgaga agcgatttct ggaatgtaat agcctttttg agtcacgccc caaacgatcc
    26401 agacaaggac aactaacatc tctaataaga tcaatttatg cccaagcatg aattttttat
    26461 tttcatcaga tgtctttttg atatgagaac ggaaataatc atctgatttg taagtcaacg
    26521 aactttctgg ttttttacgg attttttctg catatatcat catataacca gcggctaatg
    26581 cagtaacaac tacccacata atcaatcgga atgtcgcacc agacaacacg gggattccag
    26641 caatcccttg agccaccgct acgctaaacg ggctcatcca agaggtcgca tttccgactt
    26701 gagatgcgac ataagtcacg gttactgcta cgatcgagtc atatcctaac gcaatgacaa
    26761 atggaaccat caccattgag aatggaatta cctcttctgc catcccgaat gtagcgccac
    26821 cgaatgaaaa agcaaagaat aatagcggta aagctaaacg ctccagtccc ttagttttac
    26881 tgataaacgc atagatacct gcgtcgatcg ctccggtacg cataataata ccaaaagcac
    26941 ctccaacaac aaggatcaat gcagcgatcc ctacagcaga tccatactta tctccagata
    27001 ctagtccttc aaaaacaaaa tttaagaaac caaagccgcc aaaatcatct gttccccata
    27061 ttttggctgt tttatgcaat tttttagaag tatcatagat attttcgccg tactgatcgt
    27121 aaagcacatc atctgtcaag gagatttcat ctagtttttc ctgcgtaaga tttttttctc
    27181 cgtcagcaat gactttttcc aatccttttt ccggaacatc cagtttttca cgttctgctg
    27241 gatgatcttg cagttcttct aattgatcaa acacatacga cttatccagc tcgtaagcat
    27301 aacggaagga atcttgacgt aaaacagtac gagtacttgt ttcgccattt gcatctttgt
    27361 attcaatatc ttgcgtgctg aactttcctg ctggaacgac aaaagtcagt atccatgcaa
    27421 agatgactac accaaaaata atgacatacg tatggggtgt cgataattta gtgaagtcgc
    27481 gtttcggttt tcctgaagaa tttgttttct tcaaatttat ttcttccttt cttttttcag
    27541 taattttgat gaataaaaaa tggataatca aatgaattat acagt
//
LOCUS       DACCQI010000026        27354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCQI010000026 DACCQI010000000
VERSION     DACCQI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.026"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            254..484
                     /locus_tag="IVO76_000880"
     CDS             254..484
                     /locus_tag="IVO76_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7428862.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL"
     gene            484..825
                     /locus_tag="IVO76_000881"
     CDS             484..825
                     /locus_tag="IVO76_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7428863.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSIGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQLKSLDFKERRLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            1520..3601
                     /locus_tag="IVO76_000882"
     CDS             1520..3601
                     /locus_tag="IVO76_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7428864.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAERLAAEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFSLKLENDKQVRLETEIKEVKDGFLNRRERIFDLVLAIAYS
                     RNPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFFHYFPLAEIKKIEEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDYMITEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHIPFHLKEEALFENLYKHMAA
                     LIIRIKHAIQVMNPLKENIRTNYFPIHRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYQSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVDLVFSTFQLNYRNKPLLVIDPILTETNILVIEDFLKRNEELQRLKPNSHESTQLFT
                     QIIGVMEESGGRVDRIIYGKLEKLFEINNLEINKREIQPMLKDILTDSNIMIKEKAAT
                     WEETIERAAKPLVKENIVEERYVEAMIRAVNEYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NVEDFKQLLFQ"
     gene            3637..3939
                     /locus_tag="IVO76_000883"
     CDS             3637..3939
                     /locus_tag="IVO76_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7428865.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFSVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            3965..5254
                     /locus_tag="IVO76_000884"
     CDS             3965..5254
                     /locus_tag="IVO76_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7428866.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEEIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPILKAF"
     gene            5273..5680
                     /locus_tag="IVO76_000885"
     CDS             5273..5680
                     /locus_tag="IVO76_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7428867.1"
                     /translation="MFGFFKKKENEPKKRSIKEEKILSPEVTEKIQSEITQLNQEIST
                     TQEKPKLAELYEQVGLKFSELYENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDVMRQIAKQVTIKGNK"
     gene            5703..6572
                     /locus_tag="IVO76_000886"
     CDS             5703..6572
                     /locus_tag="IVO76_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7428868.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKGTFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            6574..7194
                     /locus_tag="IVO76_000887"
     CDS             6574..7194
                     /locus_tag="IVO76_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7428869.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SVGFNNLEERAKQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKTRLQELAERY
                     GYQVMTIRLTADFEVLWQRRYKRDREPDRHLSYIMDHYHYGDILEDRSKGTNHITKEA
                     FKKIITDRKYDEFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            7391..7996
                     /locus_tag="IVO76_000888"
     CDS             7391..7996
                     /locus_tag="IVO76_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7428870.1"
                     /translation="MNNKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTVIEEGHPTPKVDVRAYIRKDGKILLIEDKRTREWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVESTQLQAVFDTNKQKDIPQLFQYYKLVFACTIHDEDAKFI
                     ENNETSDMGFFSIEELPKLSEKRTTKKQLLILENKNQLYCD"
     gene            8132..8584
                     /locus_tag="IVO76_000889"
     CDS             8132..8584
                     /locus_tag="IVO76_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7428871.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLKKVCKNVSEDWNFVKKGNKRIY"
     gene            8597..9117
                     /locus_tag="IVO76_000890"
                     /pseudo
     CDS             8597..9117
                     /locus_tag="IVO76_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9135..9845
                     /locus_tag="IVO76_000891"
     CDS             9135..9845
                     /locus_tag="IVO76_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428872.1"
                     /translation="MKKVIVDANFWEVFPEATIEILSVSGIDNHVTEENEEMYHQLLN
                     SAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKISGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVSIVRHTLTKDESEAEL"
     gene            10041..10409
                     /locus_tag="IVO76_000892"
     CDS             10041..10409
                     /locus_tag="IVO76_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7428873.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGIFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMDEPGEITDNNGVLT
                     FNFSDPEGNYFVIGKAESSD"
     gene            10429..10653
                     /locus_tag="IVO76_000893"
     CDS             10429..10653
                     /locus_tag="IVO76_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428874.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRARNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKVKSHY"
     gene            10897..12210
                     /locus_tag="IVO76_000894"
     CDS             10897..12210
                     /locus_tag="IVO76_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7428875.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALSGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAMLLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGKLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            12538..14175
                     /locus_tag="IVO76_000895"
     CDS             12538..14175
                     /locus_tag="IVO76_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7428876.1"
                     /translation="MERKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDVIWLSPVYESPNDDNGYDISDYQAIMAEFGTMEEMEELIEEAKKRNIRIVMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGEEPNGLRSIFSGPAWQLDEQTGQYFL
                     HLYSKRQPDLNWKNKQVRQAIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSDPKRKELSMIFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDLYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDISEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEETPEHVFAYSRIYEDQRWLVVCNMSSEKQVFTVPRE
                     ALRIIVSNYPLKQLPDGKVELRPYEAFVMEVNKEKLI"
     gene            14323..14793
                     /locus_tag="IVO76_000896"
     CDS             14323..14793
                     /locus_tag="IVO76_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7428877.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRLLNKLADEAFRKTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FTKEVDGSWEELHQSYKAVLGNEASKELAEIMNEATDKLQKNKNFFKQISCMCN"
     gene            14859..15950
                     /locus_tag="IVO76_000897"
     CDS             14859..15950
                     /locus_tag="IVO76_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7428878.1"
                     /translation="MSQFDQSIILPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIVQIF
                     HGGRMTSRATLSGEQPVSASAVAAERPDAETPRALSENEIKETIQAFGEATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIDELLTTVFSTVEKYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRETKLDYLHVSLNAYDRRARSDKYSDQSI
                     LEAVHDTIQGKIPLIGVGGIRTRKDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            15980..16657
                     /locus_tag="IVO76_000898"
     CDS             15980..16657
                     /locus_tag="IVO76_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7428879.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYKDEDFRNRALGNTLRPDDVDAKDYAAVYYAGGH
                     GTMWDFPEATDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGADYQSGPAFSEVVQIDGRLLTGQNPQSAHQLGEV
                     VVEALKH"
     gene            17002..18297
                     /locus_tag="IVO76_000899"
     CDS             17002..18297
                     /locus_tag="IVO76_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7428880.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLIPTSEIYLIEKQSKLGWIPSGF
                     NLIVKGKVTSEKQLSWITKEELIDRYRIHVYTEKTVIGLEEKKVVLAGGDKLDFDRLI
                     LATGSNQNFRNVPAEARFIHPVKKIQNIAALQQKIMDAKKIAIIGAGQAGIEIAEGLA
                     SGKTQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGISLFLEEQVVSLSENETAVVET
                     ERRRENYDLVLLANHSRPNNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSATKDQGTYRPVGNQWFGYFLGSVGMTEE
                     ESIFYPQKIETGYAEYKLAAINQSKVKVKFLFDSEKKILGAQLQSRKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPNMVWSKVANTAYED"
     gene            18287..19747
                     /locus_tag="IVO76_000900"
     CDS             18287..19747
                     /locus_tag="IVO76_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7428881.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKTSF
                     DQYLEDIELIGRMMGKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLGRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYETLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLEKLQETAMQQLPRKSTEDSALSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLNRELRGVSGIEIESYEEGKNYDLVITF
                     CQRNKQQNEYYLSEFASPYDIIRLKRRIEMLKKEKN"
     gene            19884..21380
                     /gene="glpK"
                     /locus_tag="IVO76_000901"
     CDS             19884..21380
                     /gene="glpK"
                     /locus_tag="IVO76_000901"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7428882.1"
                     /translation="MTEPTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQDILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEAAA
                     KRSTNEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            21394..23217
                     /gene="glpO"
                     /locus_tag="IVO76_000902"
     CDS             21394..23217
                     /gene="glpO"
                     /locus_tag="IVO76_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7428883.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQAAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQGIAPHIPKPDPM
                     LLPIYEDEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYEGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LADIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVRELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMTPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAEYFGWTEEEKVQQTKRLEALIAESDLRELKGEK"
     gene            23218..23934
                     /locus_tag="IVO76_000903"
     CDS             23218..23934
                     /locus_tag="IVO76_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7428884.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            24240..26168
                     /locus_tag="IVO76_000904"
     CDS             24240..26168
                     /locus_tag="IVO76_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7428885.1"
                     /translation="MNNEMISQLQFDQIKKEIQARAIGNYSKKRISKMTTSSNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLVLSPADLIEYADFLRSSRMI
                     TKFFDKNQYQAPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQII
                     EKEIQSKLLKFLRHPKNKEMIQEAIIVQKGECYTIPIKASYKNKVDGTIIDESNKGTT
                     VFIEPTVVSKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEAIDLLIETMTVLDIIF
                     ARAKFSREINGITPKINKSEHIVIKQGRHPFLPDHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            26294..26848
                     /locus_tag="IVO76_000905"
     CDS             26294..26848
                     /locus_tag="IVO76_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7428886.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         9167 a         4613 c         5974 g         7600 t
ORIGIN      
        1 tagatgaact agcaagtttg attgcagact gtagacaaag gtatcccttg cctctttacg
       61 aagagtgtag gttgttactt ttgtctattt ttgtatagaa atagaaaaca tttgtctcca
      121 tttccaagct gctaattttt caaagtgcac catttctcaa aatgcttttg atcatttctc
      181 catctagttt acaaagggta cttttgcgtt acttagtgtt gtgtagtata cttttactga
      241 agaggtgatg atgatgcttg ttacaaaatc tagattacaa ggaagttctg tagtggttac
      301 tttaccttct gataacggaa agaaaccttc agaaaaccaa gaatatatcg ttgtatattc
      361 tgacgatggg acaattacgt tagttccaaa aattgaagat ccatttagtg gtggagaaga
      421 ggccgaatat tatgaaaaag acgaatggga agatttaact cctgaaggga gagaaaggtt
      481 atagtgtcag aagaaagaat ttatattcct aaaaaaggtg atattgtgtg gattgatttt
      541 gatccttcga taggaaaaga aattcaaaaa agacgaccag gactagtcgt ctctcgttat
      601 gagtttaatc gtaaaacaat gttttcggtc atatgtccga ttacttccac gataaagaat
      661 ttaccgacac gttactcatt accagaagag ctagatataa aaggacaagt acttatttct
      721 caattaaaat cacttgattt taaggaacga agactcaaaa aaatagaaaa cctgccattg
      781 caagacatgg ctaaaattga tcagattata caatatatgt tttaagcgtt gtaactaaac
      841 tctacaatat ttttatatgc taaacttaac aaaatttttt gacatttaaa aagaaaagtg
      901 acaataaaaa atagcatcta aatagcaatg acccccaaat ttaactcttg gggggacatt
      961 tcaaaaggga taagaaaaca aacattctcg tcgtatctga ctagtgaaaa agatggcagg
     1021 ccgattttat tcagccgatt atgctggatt gacaggaaat atttatgaat ggagctgtga
     1081 caaaaatttt gtcgcagctc ctttttctga ataaacggtg ttccagagat agcctatcgg
     1141 caataaggca cgattttgta aaatatggga agctattttt caaaatcgct tcttatcgct
     1201 caaggctgaa cctctccgtc acaacctttc cactaaatgg tattcaaaag cttacattta
     1261 cggtaataag ctcaaccaaa cgaaaaatag gaagcactat ttttgtttgg ctgttcttct
     1321 tacttgatgc aggacagctt tttcatgacc tcttttttgt aataataggc gtatgtcttt
     1381 ttcgttgaga agttgaaaaa gcatacggct tattttttgt ttgctatgct tactttcttt
     1441 ccacttcaag tggcagaaat atctactttt tattatgtaa gcggaaccat atacttggaa
     1501 tcagaaggag atcatttcta tgaaaacaag aacaattgaa atacttcaca gaatcatgac
     1561 agcccaatat ccagtaaaag cggaacgtct agcagctgaa ttcaatgtca gtcagcggac
     1621 gatccgtcag gaagttcttg aagcgaatat ctggttgagt agtaaaaagc ttccggaaat
     1681 cagaacaatt cgcaacaaag gtttttcgtt gaaactggag aatgacaagc aagttcgatt
     1741 agaaactgaa ataaaagagg tgaaagacgg ctttttgaat cggcgggaac gtatttttga
     1801 tttagtttta gccattgctt acagccgaaa cccgatccat ctaaacaaaa aagaagaaga
     1861 atttcaaatc tctaaaagta cgttagatga agatatgaga cgattacgag caacactggt
     1921 cagttatgga atcgagatcg tcagttatgg aaagcaggga ctggtttata aaggttcaga
     1981 acgatctatc cgtacgatga tctatgattt tatcaatcag aatctgggga tgaccgactt
     2041 ctttatgaat caagatgcac aactaacacc ggctcaaaaa atctttttcc attattttcc
     2101 tttggcggaa atcaaaaaaa tagaggagat ctattccaat aagttgttaa aacaagagga
     2161 tgatatttat aaaaagcaaa ttctgctttt tacattgatc tggatccaac gaatcaggaa
     2221 acatgaattc atctctgcca ttaattggaa aaatacagaa ataaaagaca gcagcttctc
     2281 tgcatttatt gactacatga tcacggaatt tcaattggac aatgttccac aagtagaaag
     2341 aaattatctt cgtttcacta tcgagacgtt caatgctaga gatatctcca attcgattga
     2401 gtgggtacaa gctcaactac taacgatcca attgatccaa tttgtagaga acgagacaca
     2461 catacctttt catctaaaag aagaagcttt attcgaaaat ctctataaac acatggctgc
     2521 actgatcata agaatcaagc atgctattca agtcatgaat ccattgaaag aaaacatccg
     2581 tacaaattat tttccaatcc accgcgcagt cactcatttt gttcctacga tcgaagaagt
     2641 aatcggcggc aagatactgg aggacgagat agcttttctt gtcatccact tttcgacgat
     2701 tgccagtgtg atcaagcgag acctgaatta tgtttatcaa tcagttgttg tctgtaatca
     2761 cggcatggct accgggaatc tgttagcaga aaatctgaaa gaaaaatttc ctcaaatcga
     2821 gattactgca gttttaagtt caaaagaaat cgaattagtg gataaactgg atgtcgatct
     2881 ggttttcagc acctttcaat tgaattatcg aaataagccg cttttagtta tcgatccaat
     2941 tttgactgag acgaatattt tagttataga agactttttg aaaagaaatg aagaactgca
     3001 gcggttaaaa cctaacagtc atgaatcaac gcagttattc actcaaataa tcggtgtcat
     3061 ggaagaaagt ggtggccgag tagatcgcat catctatggc aagttggaaa aattatttga
     3121 aataaacaat ctagaaatca acaaaaggga gatccagcct atgttaaaag atattttgac
     3181 ggacagcaat atcatgatca aagaaaaagc cgctacatgg gaggaaacga ttgaacgagc
     3241 tgctaaacca ttggtcaaag aaaacattgt tgaagaacgc tatgtcgaag cgatgattcg
     3301 cgcagtgaat gaatacgggc catatatcgt gattggcaaa cacatggcgc tagcccatgc
     3361 tcgaccagaa gatggggtca ataagttagg agtcagcgta gctacgattg agcaaccaat
     3421 cgatttcggt aatcctgaaa tggatccagt aaagattatt ttttgtttag cggcagtaga
     3481 ttcctactcg catctgaaca tcatgaaaga actgatcgaa ctgatcaaca atgaagagaa
     3541 gttggatcga ttgatcgagt gcacaaatgt tgaagatttc aaacaactgc tttttcagta
     3601 gttgagaaat aataaatgat acgggggaga acaaagatga acaaattaaa cattttattc
     3661 gtatgcggcg caggtttagg aagtagtttt gcagcacaga tgtctgctga ggatgttttg
     3721 aacaaattag gagtagaagc caaactggac cattgtgata tttcttctgc agtgtccatg
     3781 aaaccagatg tgatcatcac agcagaaaat tttcgctcgc agttcgaaaa attctcagtc
     3841 gatcctaaaa cagcgatgat ctatttgaaa aatattgtct cgaaaaatga aattgaagaa
     3901 aaaatcaccc cagttctaca agataaggga gttatctaaa taaaaaagaa aagaggataa
     3961 aaacatgggc gttatcaatt ttatcatcga aaatattttg actcaggcat cgatcaccat
     4021 tgcattgatt gctatgttgg gtctactgct gcagaagaaa tcagcaggac aagtcatttc
     4081 tggcacgtta aagacactcc taggattcca agtgttgagt gccggttcga gtatcatcgt
     4141 tggaagttta acttatttcg ggaaaatttt tactgaagga tttcatatgc aaggaatcat
     4201 tccttctatt gagtctatca atggtcaagc aatgaatgat ttaggtttag gtagagatat
     4261 tgcactgacg ttcttagcga tttttgtatt caatattatt ttggctcgtt tcacaaaatg
     4321 gaagtacatt tttctaactg gtcaagccat tttatggatg gcgacgatga cgacggtctt
     4381 tggttatttc gctggtctgc gagggatcgt attgatctta gtcggtggtt ttatcggagc
     4441 ttgtttcgcc attgctatgc cagcagtagc gcaaccaatt atacgtaaga ttacaggatc
     4501 gaatgatatt gctttagggc atttttgtac gattggatac ttatttgaag ctggcgtggc
     4561 taaacttttt ggtgagaaag gtgaaaataa aaaatcgatt gaagagatca aattgcccac
     4621 gcatttcgag tttctgcaag atacgtattt gtctgtcatg gtcgtgatgg tcccactata
     4681 tatcatcact gttttgtttg caggtgaacc attcgcttct gaattatcag gagatcaaaa
     4741 ctatatcatg tttgcctttt tacaagccat ccaattcgta gtgggtgtgt atgtattgct
     4801 agctggcgta cgtctactgt taggtgaaat cgtgccggca tttcgaggga tagctatgaa
     4861 attagttcct gatgcgattc ctgccttaga ttgtccagtt ttctttccct atagcccaaa
     4921 tgctgttatt ttaggtttca ttacgacaac aatcggtacg attattgcca tgttcacttt
     4981 gccgatgttc ggcttagcga tgattttacc aggtatgcta acgaatttct ttgccggagg
     5041 aacagcgggg atatttggta acgcagttgg cggacgtcga ggagcgatca ttggcggtat
     5101 tgctcatggc ttctttatta cattattgcc tgctttgcta gtaacgatct ttaattcgat
     5161 gggattcatc aatgctacag caaccgatgt agatacagta gccgccgcac tcttatacgc
     5221 ttggatctta agcccaattt tgaaagcatt ttaattgaaa gtagggaaaa tgatgttcgg
     5281 attttttaag aaaaaagaaa atgaaccaaa aaaaagatcg ataaaagagg aaaaaatcct
     5341 ctcgccagaa gtgaccgaaa agattcaatc agagattaca caattaaacc aagagatttc
     5401 aacgacccaa gagaagccca aattagcgga actttacgaa caagttggac ttaaattttc
     5461 tgaattgtat gagaacgatc aagccatcca atatttagaa aagagcttgg aaaataaaca
     5521 gacgatcgga gatgggtata aaaaactgat gagcctctat aatcagaagc gtgcagatgc
     5581 tgctcgtgcg ggtgatgatc aagggatcga ttactacatg ggcaaaatgg atgtgatgcg
     5641 acaaattgca aaacaagtaa cgatcaaagg aaacaaataa aaagataagg tggcaataaa
     5701 aaatgtatac aacattgaaa gaagtaacaa aaacagcaga agagttgaat tttacagttg
     5761 gagcttttaa tacgcataac ctagaaatgc ttcctgccat gctaggcgca gcaaaagaaa
     5821 tgggttcgcc aatcattatt caaacaagtg tcgacaccgc aaaatatatc ggtatgcatg
     5881 tgttagtcaa tgtaatgaca gctattgcag aagaagaaat ggttgatgca gtcttgcact
     5941 tagaccacgc ccgagacttt gatgatatca aagaagcaat cgatagcgga tatacttctg
     6001 tcatgtatga cgggtcccat ctgcctttca aagaaaatat cttgaagacg aaagcagtgg
     6061 tagaatatgc ccatgcacac ggtgtttccg tagaaggaga acttgggacg atcggcggta
     6121 cagaagaagg aatccatgtc gctgaaaatg acaaagtata tacaagaccg gaagacgcag
     6181 tgaaatttgt agaagctaca ggtgtggatg cattagctat tgctatcggt acgaatcatg
     6241 gacaattcaa atcaaaaaca gaagtgaaca ttcccttgct gaaagaaatc gatgcagtag
     6301 tcgatgtccc cctcgttatc catggcggaa caggtgtgaa agaagaagat tatcccgaat
     6361 tgatcaataa cgggatccgc aaattcaatg ttggaacgga gcttttagtc aactggacaa
     6421 aagtggctaa aggaaccttt agtgaaacag aaatcaacaa gtctcttcgc cataatgtga
     6481 ttccagcaaa tgaagcagta aaagaaattg tcaagcataa gattggactg tttatgaact
     6541 tggaaagtcc aattaactta ggaaacaaat gagatgaaaa agtatttcat tcttctagct
     6601 ggtagtcctg gtacaggaaa aacatatctt atgaataaac tacgggaacg atttccagat
     6661 atgtatgcca tcacgctcga tgaaatcaag gaatattatg cagaatccgt cggttttaac
     6721 aacttggaag aacgggcgaa acaagaaagg acgaaagttt atcctttcta ttacaaagca
     6781 cttgatctgt atatggaagc aggaaagaaa gtgattgtgt cagagtatcc ttttagtgat
     6841 aagcaaaaaa ctcgactcca agaattagca gaaagatacg gatatcaggt aatgacgatt
     6901 cgcctaacag cagactttga agtgttatgg caacgtcggt acaaacgtga tcgtgaacca
     6961 gatcgtcatt tgagctatat catggatcat tatcactatg gcgacatatt ggaagatcga
     7021 tcgaaaggga cgaatcatat tacgaaagaa gcattcaaaa aaatcatcac tgaccgaaaa
     7081 tatgacgaat ttcagttagg aacactatat gaatttgatg taacagacta tactcaagta
     7141 gattacactc cactattgga taaattagaa aaagaaatca atgacaataa gtaacgaaga
     7201 aatggtttga tagtgacagg taataaaaaa gacatttggt ccccagagga gccaaatgtc
     7261 tttttgttct aaaatgtttt ttcatattct tttatttatt gccgatttcc ttgtgtttat
     7321 ttgccgtaat taggtcagtc ttctatactt gattacatag tcatacattt gctgaaaaga
     7381 agggaagaga atgaataaca agttggccca atacaaaaga ctgctcgcac tttctcaagc
     7441 tggtctgtat tatggaaaag atgtattcga taaagaacga tttgaagaga ttcggacgat
     7501 ctcattggaa ttgatcagtg aaataggaaa agaaactttc gaagaaataa aagcacttac
     7561 agttattgaa gaaggtcatc ccacaccaaa agtcgatgtt cgtgcctata tcagaaagga
     7621 cggcaagatc ctactaatcg aagataaacg gacgagagaa tggtcactgc caggcggatt
     7681 tgccgaaata ggtttgtccc ctgaagaaaa cgtgcggaaa gaagtctatg aagaaacggg
     7741 tttaacagta gaatctactc agctgcaagc ggtttttgat acgaataaac aaaaagatat
     7801 tccgcagctt tttcagtatt ataagcttgt ttttgcttgt acgatccacg acgaagatgc
     7861 aaaatttatt gagaacaatg aaacttccga catgggattt tttagtatag aagagctgcc
     7921 aaagctatca gaaaaacgaa caacaaagaa gcagttgctt attttagaaa acaaaaatca
     7981 gctctattgc gattgagttt tttcgtaaaa gaagaaagct gtttactctg cgagttctct
     8041 aaaaaagaac tcgttctttt attaaaaaat agttgcattt gcaacaaaaa gaggtaaact
     8101 agtcttacaa acaaaataaa ggtggggtga aatggctgaa attcttcgag acattggaat
     8161 gattgcccga gcgttggatt ctatcagcaa tatcgaattc aaagaagttg atttaacaag
     8221 agggcagtat ctttatttag tacgtatttg cgaaaatcca ggaatcatcc aagaaaaact
     8281 agcagaaatg atcaaagtag atcgtacaac gactgcacga gcaatcaaga aattagaatc
     8341 caatgggatg atcgaacgtt tggaagataa agaaaataaa aaaatcaaga aactttatcc
     8401 gactaaaaaa ggagcagaaa tctatccttt catcatccga gaaaacaact actctaatgc
     8461 agtcgcatta aatggtctgt ctgacgagga agcaaaacaa ttggaatatc ttttaaagaa
     8521 agtatgcaag aatgttagcg aagattggaa ttttgtcaaa aaaggtaata aaaggatcta
     8581 ttgaaaggaa gaagccgtga ataaatacga aattcgagaa atgacgcctt cagatgtaga
     8641 cttactacaa caaatcagta tcgaaacatt tacagataca tttggaaaag ataattcgcc
     8701 ggagcattta gctgcttact tgagacaagc ttatgcgcca gaaaaactta cagaagagct
     8761 aaaagagaaa gaaactgatt tttactagta agtatggacc aagagcctgc gggttacctg
     8821 aaactaaata taggaacgag tcaatctgag gaaatggatg atcatttttt agaaatcgag
     8881 cgaatctatg tacgaaaaaa atttaaacat caaggtattg gtacattttt actagataca
     8941 gctatcaaaa aagcaaaaga aaagcaaaaa acagccattt ggctcggtgt gtgggaacac
     9001 aacgatgctg cgctcgcttt ctatcgcaaa agaggattta ccaagtacag tgaacatgtt
     9061 ttttatatgg gagatgatcc ccaaacagac tatctattca tcaagcaatt attctaatca
     9121 agaggtgtga acatatgaaa aaagtaatag tggatgccaa tttttgggaa gtatttcctg
     9181 aagcaaccat cgaaatctta tccgtgagtg gtatcgataa tcacgtaaca gaagaaaatg
     9241 aagaaatgta tcatcaatta ttgaatagcg cggcaaaaga agcaagaaat taccttacgg
     9301 aagaaacatt cagccaaaac gaagtcattg ctcagtggcg tcaggctttt accacattca
     9361 agactaaaaa aggtgcacgt tcttctattg aagctttact gaaacgtgca aatcaagaac
     9421 gggaattttt cccaatcaat ccgcttgtag atatttataa tagtatttcc ttgtcttttg
     9481 gcgtaccttg cggcggagaa gatatcgaaa agatttcagg aaatctgcat ctaggaaaag
     9541 cccaaggtgg cgaatcattc ctgcctttag gtgcagattc cgatgctccg gcacttccgg
     9601 aagaaatcat ctactatgat gaacaaggag caatctgtcg ctgtctcaac tggcgtgaag
     9661 cccaaagaac gatgctgacg gaagaaacgc aagcagcggt gctggtcatt gaatccatca
     9721 atgctgaaca agcggcacgc ggcgcacagg caatcgaagc gttgaaggaa aaaatcgatc
     9781 aaacatttgg cgtgtccatt gtcagacaca cactaaccaa agatgaatca gaagcagaat
     9841 tataaaagaa tcgaaaaaag aagagcggca ttcattgccg ttcttttttt gtaaaaaaag
     9901 tttgtggagg aaagcaagtc tagaaactat tgctttattt ttcttgaata ggatcaaaca
     9961 caaggtgttc gcgctttcgt gatataatgg aatcagttta tagacaggaa agaaaatcaa
    10021 ctagaaagaa gggtcaaacg atgatacaaa aaacacgtgt gatgctttat gtcaatgacg
    10081 tagagatgat ttgccgattc tttgtagaaa aaattggtgc agaaatttca gaaacgatcg
    10141 aattgccaga agaatttaag agtattgtgc tgagtatctc aaaagaatta gaattaggta
    10201 tatttccaaa agtatttgtg cagaagtttt caccagaagt attgggaccg ccaccttctt
    10261 tagtcttttt tacggatgaa tttgaaacta tttacgagaa catggacgaa cctggagaaa
    10321 tcacagacaa caatggcgtc ttgactttca atttttcgga ccctgaaggc aattattttg
    10381 ttataggaaa agctgagtcg tctgattgaa taaaaaggag gaaaaacagt ggatctaatg
    10441 ggaaggctag aaggttattt gaagtatcgg cgagcaaaac atagtgagaa gcttcgagca
    10501 aggaatctca aatcagggaa aaagaagaca aaaggctggg gaaaaatggt cgatacaggt
    10561 tttggcggac taaataacct aaatcctgtc gatacaaaag aattcatcaa agaagagcag
    10621 aaacgaaatg gtaaagtcaa atcacactat tgattttgat actagaatga agaaactttt
    10681 ttaacacaat aaagaaattc ttttgatcca aagtgaatgt tcatggactt tgagataaag
    10741 ggatttgaac agatggaacg ttcggatttt gcttaaaatc cgaatttttt gtttacttat
    10801 gttttgtttt agtatatgat aaaacacgtt gttttattaa tcaatcgaaa ttttaacgga
    10861 ctttatttat aatgttcgga tttaggggga tggaagatga aggaaaaaat actttcctac
    10921 ttcgaaatag aaaaattgaa taccacggta aaacgtgagg tactggcagg atttacgaca
    10981 tttatttcaa tggcctatat cttatttgta aatccaactg tattaggcgc ttctggaatg
    11041 gatgaaggag cggtgttcac tgcgactgca ttagccagtg caatcggctg tattttgatg
    11101 ggggtattag cccgttatcc aattggaacg gcacctgctc ttgggatcaa tgctttcttt
    11161 gcttattctg tttgtttagg gatggggatc ccatgggaaa cagcattgtc cggcgtattc
    11221 gttgcttctt tgatctttat tttgattaca atcttcaaac tgcgggaaat gatcattgat
    11281 gcgatcccag cagatttgaa atatgcgatt tctggtggta tcggtctatt tattgctttc
    11341 cttggtctga gcgaaggtgg gatcatcgtt tcgaatgaat ctacattagt aggattagga
    11401 tcactcaacg ttggttctac ttggttgacg atttttggat tagtcgtcac ggctatgttg
    11461 cttgttcgac gtgtaccagg tggcattttc atcgggatgg cagctacaac gattttaggt
    11521 ctggtcacag gtttgattcc aatgccttct gaaattgttg ctgcagcacc aagtttgaaa
    11581 ccaacatttt tagttgcgtt aaaacatgta ggggacatca attctcttca aatgtgggtc
    11641 gtagtactaa cattcttgct tgtgactttc tttgatacag caggaacttt agttggatta
    11701 gcaaatcaag caggctttat gaaagacaac aagatgcctc gtgtagggaa agctttagct
    11761 tctgattcaa cagctatgct tgcgggctcc ttattaggta cttctcctgt tggtgcattc
    11821 gtagaatcat cagctgggat tgctgttgga ggacgctcag gaatcacagc tattactaca
    11881 gggattttct tcttgttagg tttgttcttt tctccattac tgtcagttgt tacttcacaa
    11941 gtaactgcac cagcattgat catggtaggt gttttgatgg ctcaatcatt acgtcaaatc
    12001 gaatggggaa aattagaaat tgcgatccct tccttcttga tcttgattgg catgccttta
    12061 acttatagta tttcagatgg gattgcatta ggttttattt tctatccaat tacgatgtta
    12121 gcggctaaaa gaggaaaaga agtatctcca atcatgtatg gtctgttctt tgtattcgtg
    12181 ggattcatgt ggatattgaa tgtgaaataa agaggttgtg aaatcagcgt tctggcctga
    12241 gtattagaga aaaaaacgaa aaaatagttc ctcctatttt gccgtttttg gagctaatac
    12301 cgagggacag ctgagtgaac accgtttata aagaggttgt gaaatcagcg ttcttccctg
    12361 catcaatcaa tcctttcaaa aaacagttga ggaaatttcc tcagctgttt ttttgtttta
    12421 ttctgttacc gataacaagg tgcaatgact atttgaaagc gttacaattt ttggtagaag
    12481 aaactaaact agatgattaa gcgaaaaata gaaacatcca tttataggag gaaaatcatg
    12541 gaacgaaaat ggtggcacca ttcagttgtt tatcaaatct atccaagaag ttttcaagat
    12601 tcaaatcaag atgggattgg agacttgcga gggattattt ccagattgga ttatctggca
    12661 tatttgggca ttgatgtgat ttggctttcg ccggtgtacg agtctccaaa tgatgacaat
    12721 ggctatgata tcagcgatta tcaagcaatc atggcagaat tcggtacgat ggaagaaatg
    12781 gaggaactga ttgaagaagc aaagaaaaga aacatccgta tcgttatgga tctggtggtg
    12841 aatcatactt ctgatgaaca cccgtggttc attgaggcac gtaaaaataa agaaaatcct
    12901 tatcgtgact actatatttg gcgtgatcca gtcaatggag aggagcctaa tggattgcgc
    12961 tcgatattca gtggacctgc ttggcagttg gatgagcaaa cagggcaata ttttctgcac
    13021 ttgtatagca agcgtcaacc tgatttgaat tggaaaaaca aacaggttcg ccaagcgatt
    13081 tatcaaatga tgaatttctg gatcgacaaa ggtattggtg gctttcgtat ggacgtgatc
    13141 gatatgattg gcaaagaacc ggataaagaa atcacaagta atggacctaa actgcatgag
    13201 tatcttcagg aaatgaatca agcaactttt ggcgacaaaa atctgatgac cgttggcgaa
    13261 acatggggag caactaccga gattgctaaa ctttattccg atcctaaaag aaaggaatta
    13321 tccatgatct tccaattcga acatatagga ttagatcagc aagaaggcaa agacaaatgg
    13381 gatttgaaac ctttaagtat ctctgaactg aaaaaagtat tatcgaaatg gcaaacttct
    13441 ttgggtgatg aaggatggaa tagtttattt tggaataatc atgatctgcc tcgaatcgtc
    13501 tcacgttggg gaaatgacga tttgtatcga aaagaaagtg ccaagatgtt tgctatcttg
    13561 cttcatatga tgaaaggcac tccttatatt tatcaaggag aagaattagg catgacgaat
    13621 tgcccaattg atgatatctc ggaagcaaaa gatattgaga caatcaatat gtataacgaa
    13681 aggatttctt ctggatttac aaaagaggaa atcttggaat caatcaatgc gaaaggaaga
    13741 gacaatgcta ggacgcctat gcaatggaac gcccgtgaac atgctgggtt tactactgga
    13801 attccatggc ttcgagtcaa cccaaattat aaagaaatca atgcagaaga agcgttagca
    13861 gatcaagatt ctgtttttta tatgtacaag aaattgatcg atttgcgtag aaaacatgac
    13921 atcatcgtct ggggagacta tgaattagta gaagagacgc cagagcatgt ctttgcctat
    13981 tctcgcatat atgaagacca gcgatggctc gttgtttgca atatgtccag tgaaaaacag
    14041 gtgttcactg ttccacgtga agctttaagg attattgtgt caaattatcc tctgaaacag
    14101 ttaccagacg gcaaagtcga gttacgacca tatgaagcat ttgtgatgga agtaaataaa
    14161 gaaaagctta tttgattgaa acgtattacc gagaggttgt gacacatttt ttgtcacaac
    14221 ttctttttct caaaactaaa cagaggaggc ggtcattact ccgtgttttt tgacaaaaaa
    14281 taaaaatcat actatgatat gtacgccaat ggaggggatg aaatggaaaa gccaattgaa
    14341 aaagcgttgg atgaatgtct tttctttagc gtgaaaaagc tagatcgcct attgaataaa
    14401 ttagcggatg aagcgtttag aaaaacaggt ctggcaccaa cttatggatt tattttattg
    14461 atattaaaag aaaaagatgg tataccgcaa aaagatattg cacaaatgct ctattctgcg
    14521 ccttcaacaa tcgctcgttt tgttgaaaaa ttagaatata aaggatacgt caaaacagtt
    14581 agcgaagggc gcttgtcatt agtttatctt acagaacaag gacgccaatt tactaaggaa
    14641 gtcgatggta gctgggaaga actccatcag tcgtacaaag cagtcttggg aaatgaagca
    14701 agtaaagaat tagccgagat aatgaatgaa gcaacagata agttacaaaa aaataaaaat
    14761 ttttttaagc aaattagttg catgtgcaac taaccgagtg gtttaatgat aaatgattct
    14821 cgataggtta atcagagcga attatgggag gaatgacaat gtcacaattt gatcaatcaa
    14881 ttattttacc ttcaggagtt caattgaaga accgattgat gctgtcgcct atgacgacac
    14941 aactcagctt cttcaatgga gtgatcaccg aagatgagat cacttattat aaacaacgtt
    15001 ctaaaggact tggtgctgtt attactggtg ctgcaaatgt ccaagatatc ggaaaaggct
    15061 ggccaggcga actgagcatc gctcacgatg aaatgattcc tcggttaagt gaactagcta
    15121 aagcaatcca aggagaaggt gcaaaagcga ttgttcagat tttccacggt ggacgaatga
    15181 ctagtcgagc tactctatcc ggagaacagc ctgtatctgc aagtgcagtt gcagcggaac
    15241 gacctgatgc agaaacaccg cgagcattaa gtgaaaatga aatcaaagaa acaatccaag
    15301 catttggcga agccacacgc agagcgattg aatcaggatt tgatggtatc gagttgcatg
    15361 gagcaaatac gtatttgatc caacaatttt tctctccgca ttcgaataga cgagaggatc
    15421 aatggggcgg tagcttagaa aatcgctatc gttttattga cgaattatta acaacggttt
    15481 tctcaacggt tgaaaaatat gcaaaaaaac cattcatcgt agggtatcgt ttctcgccag
    15541 aagaatatga aacaccaggt atccgttttg aagacacgat ctggcttctt gaacgattac
    15601 gtgagacaaa attagattat ctgcatgttt cattaaatgc gtatgatcga agagcccgtt
    15661 ctgataaata cagcgatcag tcgattcttg aagctgtcca tgacacgatc caaggaaaga
    15721 tccctttaat tggtgtcgga ggcatccgta ctcgcaaaga cgtagagcaa gtactgaaat
    15781 atgcagaact tgcagcaatt ggacagcaaa tgatcgtcga tcctgactgg gcagagaaat
    15841 tattggaaaa ccgtgaggat gaatttgtaa cgaaaccttt tgaagaagca tataaagagc
    15901 tttatttgcc aagtccatta tataatttct tgaaagcacg ttatcaataa ctcaattttt
    15961 atagaaagag gatggaaaaa tgaaagtatt gatcgtatta acaaatgtag aaaagtacga
    16021 aaagatcgag cgtcctacag gtttgtggtt aagtgaactg actcattttt atgatgtatt
    16081 agtaaaaaat gggatcgatg cagattttgt cagtcctaaa ggcggatatg tacctttaga
    16141 accgcaaagt gttttgaata tggatgaaat cgattgggcc tattataaag atgaagattt
    16201 cagaaatcgg gccttgggaa ataccttacg tcctgatgat gtggatgcaa aagattatgc
    16261 agcagtttat tatgctggtg gccacggaac aatgtgggat tttccagaag caacagatct
    16321 tgggaaagtt gcttcacaaa tctatcgaaa tgaaggactg gtctcagctg tttgtcacgg
    16381 tgttgtcgga ttgttgccag ttacagatga atctggaaag tctattttag ctggacgcac
    16441 agtgactgga ttcagtaatg aagaagagga agccaatggt acaacgaatg aagtaccatt
    16501 cttagcagaa gatgctttga aagaaaaagg agctgactat cagtcaggtc cagctttttc
    16561 agaagtcgtt caaatcgatg gacgtcttct tacagggcaa aaccctcaat ctgctcatca
    16621 attgggcgaa gtagtggttg aagcattaaa gcattaaaaa ttgtcagcat aaaaatgttt
    16681 tgcctattct aactattaca ttacacacgc taaaacacgt cttttcgttc ttttctgttt
    16741 cctatttgga cagaggtacg caaaaggcgg gttttttgcc tttctaagag tacatttttt
    16801 gaaacatctc ttcagaaatg atactcttca attaggtcaa ctaatttatt actgagaagc
    16861 agacctagtc acacggtagg atggacagag tgttttctgc tacgctgcta tggatcatag
    16921 gaatgagtaa acatggttta agaacattgt gatttcagcc catgttggca gtcgcacact
    16981 taataagaaa gaggaataca gatgaacatt gtaattgtcg gcgggtcgtt tgcagggatt
    17041 catgcggcta tttatcttcg ccaattgata ccaacaagtg aaatatattt gattgaaaag
    17101 caatcgaagt tagggtggat tcccagcggg ttcaatctga tagtaaaagg aaaggtcacc
    17161 agtgaaaaac aactttcttg gatcaccaaa gaagagctga tcgaccgcta tcgtatccat
    17221 gtttacacag aaaagacggt aataggattg gaagaaaaga aagtcgtttt agcaggcgga
    17281 gacaaactgg attttgatcg tttgattttg gcgactggtt caaatcaaaa ttttcgaaat
    17341 gttcctgcag aagcaaggtt cattcaccca gttaaaaaga tacaaaacat agctgctctt
    17401 cagcaaaaga tcatggatgc aaaaaaaatt gccatcattg gagcaggtca agcagggatc
    17461 gaaatagctg aaggcttagc atcaggaaaa acacaaatcc atctttatga aagccgaaag
    17521 tcgattcttt ttcgttacct agaccctgaa atgacggagc cgttagtaaa ggaaatgaaa
    17581 aatcaaggga tatcgctttt cttagaagag caagtcgtat ctttgtccga aaatgaaacc
    17641 gctgtagtag agacggaaag aagaagggaa aactatgatc ttgttttgct ggctaaccat
    17701 tctcgcccca acaatcagat gtgggaagaa caattaacat tgaatgatga tggaacgatc
    17761 tgggtagatg actatttgca aacgtctcaa aaagacgtct atgcaattgg cgatgcaatc
    17821 caagtcactt ttcgcccaac aaatgaaaaa atgtacgttt ctctagtaaa caacgctgtc
    17881 cgtacagcgc gaaatgtcag caagacaatt agtggttctg cgacaaagga tcagggaacc
    17941 tatcgaccag tcggcaatca gtggtttggc tatttcttag gaagcgtagg tatgacagaa
    18001 gaagaaagca ttttttatcc ccaaaaaatt gaaacagggt acgcggaata caagcttgca
    18061 gcaataaacc agagcaaagt caaagtcaaa ttcctatttg attctgaaaa aaagattctt
    18121 ggagcacagc tccaaagcag aaaagaaatg ttccatcttc ttgaccaact gacaatcgca
    18181 gtagaagaag gctggacgtt cgaacagtta gaagaacatg agctgttttt ccagccagaa
    18241 tatcgtgtac ctaatatggt gtggtcaaag gtggcgaata ctgcctatga agattgaagc
    18301 attattagaa agaaaagagc agttacagat cctgattttg cgaaaccttg ttctgcaagg
    18361 gggaacagcc tctatcaatg gattgagaga gcatgtccaa ctgtcaaaaa cctcttttga
    18421 tcaatatttg gaagatattg aattgattgg acggatgatg ggaaaaaaag tagaagtaca
    18481 gcgaaacgag tatcaggcgc tgcttgtgtt ggacgaagat gtcagtttgg aaaaaattct
    18541 tttgttttta ttacaggaaa gcttgaagtt taaaatgctt gtctatttat tagaacacca
    18601 gcaagtatcc atcgttcgat tagcgacagc gtttaatatc agtgagtctt ctgtttttcg
    18661 aaagatcaaa gaattgaacc agctcttaga ggaatttgga ttacaaatca aaaacggaca
    18721 attatacgga gaagagctac agatccgata tttttattat gaattattcc aatatatccc
    18781 ggaagatcaa cgaccgcttt ttttgcaaaa tacaccagaa aaaagaccat tcattctagg
    18841 attgggccgc gtattagaaa cgacgtttac ggcatcagca gaagcacaga ttgcttgctg
    18901 gctcgggatc acgaaaaaaa gactattgaa tgaaaaaagt acgtacgaaa ccttgaaaga
    18961 aaagaaatta ctctatcagt cggaccggct ttatcaagca atcgatccaa tcatcgtgat
    19021 gcatcttagt cgaacagcag cagaactgaa tgtatacgaa acaatgatgt tttacagctt
    19081 ttttgtttct ttttcgatag tcgacgaaga aatattttat caatatgatt tgacacggag
    19141 caaaaagtta cccaccgctg ttttggatac ttatattcgg gaaacgatgc tatggcatta
    19201 tcgtcctcgt cgtttgaaaa tcaaagagga aaaagcagta ggctaccaaa tttcacaaat
    19261 caacaatgaa tggtttttct ttgttggaaa gatcgaagtc tacgagcgtg atcggctttt
    19321 ggagcagcag caaaaaatgc ttgggcattc cttaacacag ttattggaga aactacagga
    19381 aacagcgatg cagcaattgc caaggaaaag caccgaagac agtgcactta gttatctgat
    19441 gatccagtat gccaatgttc ttttgatgat cgatttttat attgcgaaac cagtagtgat
    19501 agggatcgat ttagaaagtt tgcctattta ccgtattgct ttccaacagt atctgaacag
    19561 agaactgaga ggagtcagcg gtattgagat tgaaagttat gaagaaggaa aaaactatga
    19621 tttggtcata acgttttgtc agagaaacaa gcaacagaat gaatattatc tatcagaatt
    19681 tgcgtcgcct tatgacatta tccgattgaa aagaagaata gaaatgttga aaaaagaaaa
    19741 gaattgatca aaaactagca tcataataaa aaactgtcaa aaaaaagaaa aaaagaataa
    19801 aattttgaca gtttcttttt ttaaatgagt agtatgataa aagcagttaa agaaagcgtt
    19861 tgcagataat ggagggaatc acaatgacag aaccaacgta tatcatggcg attgaccaag
    19921 gtacaactag ctcacgagca attatctttg acaagaaagg aagacatatc ggcagttctc
    19981 aaaaagagtt cacgcaatat ttcccgcaag aaagctgggt tgaacatgat gcaaatgaaa
    20041 tctggaattc tgtacagtca gttattgccg gagctttcat cgaatcaggg atcaaaccaa
    20101 accagattgc tgggattggc ataacgaacc agcgtgaaac aacagtaatc tgggaaaaag
    20161 atacaggacg tccaatctat catgcagttg tttggcaatc cagacaatca gctggaattg
    20221 cagataactt aaaaaatgaa ggatatcaag aatttttcca cgaaaaaaca ggactagtga
    20281 tcgatgccta tttctcagca acgaagattc gctggattct tgatcatgta gaaggtgcgc
    20341 aagaacgcgc tgaaaaaggc gagttaatgt ttggaacgat tgattcgtgg ttagtatgga
    20401 aactgaccga tggacaagca catgttactg attactcgaa tgccagtcgg acgatgttgt
    20461 tcaatatcca tcagctagat tgggatcagg atattttgga tttattgaat attcctcgag
    20521 tgatgttacc aaaagttact tctaactcag aagtttatgg ttatacgcaa ggttatcatt
    20581 tttatggaag cgaagcacca atttcaggga tggcaggaga ccagcaagct gcattattcg
    20641 gacaaatggc attcgaacca ggtatggtca agaatactta tggtacaggt tcatttattg
    20701 tcatgaatac cggtgaaaaa ccgcaattat ccaaaaataa tttattgacg acaattggtt
    20761 acggtataaa tggaaaggtt tactatgctt tagaaggaag tatttttgtg gctggttctt
    20821 cgattcaatg gctgagagat ggtcttcaaa tgttgcaaaa ggctagtgat tcggaagccg
    20881 cggcaaaacg ctccacaaac gaagatgagg tctatgttgt tcctgcattt gttggactgg
    20941 gtgctcctta ttgggatcag gcagcacgag gggctatgtt tggtttgaca cgtggaacga
    21001 caaaagaaga tatcattaaa gctactttgc agtcgattgc ctatcaagtg agagatatca
    21061 tcgatacgat gcaagatgac actggaatca agattcctgt attgaaagtg gatggcggtg
    21121 cagcaaataa tgaatatctg atgcaattcc aaacagatat cttgaatgta ccgatccaac
    21181 gagcagaaaa cttggaaaca acagcactag gggcagcatt tttagcagga ttagctgtcg
    21241 gctattggaa agatacagac gagattcgtg aattttatga agctggcaag ctatttgaag
    21301 ttcaaatgga ggaagagcgc cgagaaaaac tttacaatgg ctggaaaaaa gcagtaaaag
    21361 ctacccaagc atttgaataa ggaggaaatc catatgtttt caaacaagac aagacaagat
    21421 agcattcaaa aaatgcagca agaagaattg gatctgttga tcatcggtgg cggaatcact
    21481 ggtgccggtg tggcagtcca ggcagcagca tcaggaatca aaacaggatt gatcgaaatg
    21541 caagattttg cagaagggac gtcctctcgc tcgaccaaac ttgtgcatgg cggtattcgt
    21601 tatctgaaaa catttgatgt ggaagtagta gctgacacag ttggtgaacg tgcagtcgta
    21661 caaggtattg ccccacacat cccaaaacca gatccaatgc ttttaccaat ctatgaagat
    21721 gaaggagcaa caaccttcaa tatgttctct gtcaaagtag caatggacct ttacgacaaa
    21781 ctggcaaatg tgacaggaac taaatatgag aactataccc tgacaccaga agaagtattg
    21841 gaaagagaac catttttgaa aaaagaaggg ctaaaaggtg caggtgttta tctggatttc
    21901 cgcaacaatg atgcccgttt agtgatcgat aatatcaaaa aggctgcaga agatggggct
    21961 tatctagtaa gtaaaatgaa agcggttggc tttttatatg agggcgatca aatcgttggt
    22021 gtcaaagctc gtgatctgct gacagatgaa gtgatagaga tcaaagcaaa attagtgatc
    22081 aatacgagtg gtccttgggt agataaagta cggaacttga attttacgcg tccagtctct
    22141 cctaaaatgc gtccaaccaa aggaatccat ttagtcgtag atgcgaaaaa actgcctgta
    22201 ccgcaaccca catacttcga tacaggaaaa caagatgggc ggatggtttt tgctatccca
    22261 agagaaaaca aaacttactt tggtacgaca gatacggatt accaaggaga ctttacggat
    22321 ccaaaagtca cacaagaaga cgtggattat ctattggatg taatcaacca tcgctatcca
    22381 gaagcaaata tcacattggc agatatcgaa gcaagctggg cagggcttcg tccactattg
    22441 attggtaatt ctggttctga ttataatggc ggagataacg gatcgatttc agacaagagc
    22501 ttcaataaag tggttgatac agtaagtgaa tataaggaaa ataaagtttc tcgtgctgaa
    22561 gtagaagatg tgttgaacca tttggaaaac agccgcgacg aaaaagcacc ttctacgatt
    22621 tccagaggta gttctttaga aagagaacca gatggcttgt tgactttatc aggtgggaaa
    22681 atcactgatt accgtaagat ggcagaagga gctttacgat tgattcgtca gctgttaaaa
    22741 gaagaatacg gaatagagac gaaagaaatc gactctaaaa aatatcagat ttcaggtgga
    22801 aacttcgatc caacgaaatt agaagaaaca gtgagggaat tagcaaaaga aggagtagca
    22861 gccggtttag aggaagaaga tgctacttat atcgctgatt tttacgggac taatgctcga
    22921 cgtatctttg aattagcaaa agaaatgaca ccttatcctg gtttgagtct cgctgagtca
    22981 gctcggttac gttatggctt agaagaagaa atggttttag ccccaggtga ttatctcatt
    23041 cgtcggacga atcatctgtt gtttgaacga gaccagctgg atgagatcaa gcaacctgtg
    23101 atcgatgcaa ttgctgagta ttttgggtgg acagaagaag agaaggtgca acagactaaa
    23161 cgtttagaag cattgatcgc agaatcagat ctgcgggaac taaaggggga gaaataaatg
    23221 aacagtgata tgactcaaat catgggggag tttattggga cattgatcct agttttgctt
    23281 ggagatggtg tgtgtgcggc tgtgaatcta aacaaaagta aagcacaagc ttctggctgg
    23341 attgtcattg cgtttggttg gggactcgct gtaacaatgg ctgtctatat ttctggattc
    23401 atgggcccag ctcatttgaa tcctgcagtc tctctagcta tggcgatgac aggggcaatt
    23461 agttggaatc ttgtcgttcc tttcatcatt gcgcaagttt taggagcttt tgcaggggcc
    23521 attcttgttt ggctttctta tttgccacat tggaatgcaa cgaaagatga aagtgcgatt
    23581 ttgggtacgt ttgcaacagg tcctgctata cggaactatc cagcaaatgt aatcacagaa
    23641 ttaattggta cttttgtatt agttcttggg ttgctagctt ttggacaaaa cgaatttgca
    23701 cctggaacga acgtattcgc tgtaggtgga ttgatcttag ccatcggtct ttcattagga
    23761 ggaccaacag gatacgcaat caaccctgct cgggactttg gtcctcgatt agcacatgcg
    23821 gtattgccaa tagctaataa aggaacttca gattgggcat attcatgggt accaatcgca
    23881 ggtccaatga tcggagcaat tatcgcggta ggtgtctatt cattaatggt gtaaatctat
    23941 aaaacatact aaggtttgac aatcaaaagg cgtcttgcta tagttaagtg gaaaataaat
    24001 aagacctaga agaatgggga atgttttgtg agtgtagctg gtatctaatc agtaagtcga
    24061 atagccaata aacaaggttt atttggtgtg cttatgaaac ggtacctgtc aggaaaacaa
    24121 tatctttgta ttctaataag gataggacat gactttttta agctgtgttc tatcctttta
    24181 tttatctcta ggcccttatt tattttggga caggtatctc ttcaagaaaa ggagagagta
    24241 tgaacaacga aatgattagt caattacaat ttgatcagat caaaaaggaa atccaagcaa
    24301 gagcaatcgg aaactatagc aaaaaacgaa tcagtaagat gacgacctcg tcaaacctac
    24361 aaaccgtctt ggatcgtcaa gaagaaacaa gagaagcacg cttgatctta gaaagtagtc
    24421 agcatgttcc atttatggga ttgccgagga ttgatgcact gacagaacaa gtcaaaaaag
    24481 gattggttct gtcgcccgct gatttgatcg aatatgcgga ttttctaaga agcagtcgga
    24541 tgatcacaaa gttttttgat aagaatcaat accaggcacc tttgttattt gcttatagca
    24601 agcatcttcc tgatttgata aatgtagaag agttgattga ccaaaaaata aaaaataata
    24661 aagtcagtga cgatgcttca cgtaatttgc gtaaagtacg caaacaatta caaataatag
    24721 aaaaagaaat ccagtcaaaa ttgctgaaat tcttgcgtca tccgaaaaac aaagaaatga
    24781 tccaagaagc aataatcgtt caaaaaggtg aatgctatac gattcctatc aaagccagct
    24841 ataaaaataa agtagatggg acgatcatcg atgaatcgaa caaaggaacg acggttttta
    24901 ttgaaccgac tgtggtttct aaactgaatg aacactatca gttgttaaaa gctgaagaaa
    24961 tcagtgaaga atatcaagtt ctggctgcat tgactggtgc tattgcagaa aatgaagaag
    25021 caatcgattt attgatcgaa acgatgacgg tattagacat catttttgct cgggcaaaat
    25081 tcagtcgaga aatcaatggg atcacgccta aaatcaataa atcagaacat atcgtcatca
    25141 aacaaggcag acatccattt ttacctgatc atgcggtacc attggatgtt gaaatcggta
    25201 aagactatcg aggcttgatt attaccggag caaatgccgg aggcaaaaca gtcgtgttga
    25261 agacagtcgg tttattgact ttgatggcga tgttcggtat gcaagttccg gcaaaagaag
    25321 ggacagagct tgctgtcttt gatgagattt tcgtcgatgt cggtgaccac caaaatttag
    25381 aaaatgcgtt gagtacattt tctggtcata tgcaaaacat cgctgctatt ttgaaaaaga
    25441 taaaaaggaa tacgttggta cttttagatg agattggcag tggaacagag ccaaatgaag
    25501 gggctgcact agcgatcgca atcatggaat ctatgtatga gcaaggggca ttgatcattg
    25561 ccacgaccca ttatggagaa atcaagaaat tcgccggaga tcatgaagat tttgtgccgg
    25621 ctgctatggc tttcgatcgg gaagcacttc gtcctaaata tcagttgcgg gttggagaaa
    25681 caggagaaag ccaagctttg tggattgctc ataaaatgga tatgtcgatg aaattgatcc
    25741 atcaagcaga gcgttacttg gcggaaaaag cctatcggac acagaaaaaa gaatttcttc
    25801 ctaagacagc agttacaaat aggaaggaga ataaaaaaga acgccagctc tttgcaaaag
    25861 gtgatcggat atttgtcaat gaataccaaa aagaagcgtt agtttatgaa gatataggtg
    25921 aagatacgat tgatgtatat ttagacaaaa aaataatcca cgtgcctcgt cagcgtgttc
    25981 gtctggtgcg ttcagcagaa gatctgtacc cgacaggtta cgatttagac agtctgttta
    26041 tcgattataa aacacgtaaa cggcagcgtg acctagagcg aggatcaaaa aaagctcata
    26101 aagtactagt aaaagaaatg cgcaaacgac aggaagaaag aagagtaaac gatgaaaaca
    26161 gcaagtgatt atctcaaatt agctcataat atcagtggct gtaaagaaaa ataaaaacag
    26221 tgaaatcatt ggttttattc cccgtgtaag ctaaaataaa aagaaaagag tgctgaggag
    26281 gtagggcgat aaaatgaaat taagcacaga ctggcgtaaa gaaatacaaa cgattccgaa
    26341 tcttttatcg atattccgta tttttttgct gccgatttat ctttattttg tgctgagaca
    26401 gtcattttat atagcaggag cggtcattgt tgtctcaggt ttgtctgatt atctagacgg
    26461 ggtcattgct cggagatata atcaggtaac agacttaggg aaggtgcttg atccgtttgc
    26521 agataaattg acacaacttt ttctgatttt gtctatggca tggtatcgac cgtggctttg
    26581 gctgttgttt ggtctattct tgataaaaga aggctttatg tttgtagctg gattaatcgg
    26641 gctatctaaa aatatcaaat taagtggagc gaagtggtat ggcaaagtag cgactgctgt
    26701 catctatgtt ggcatgatcc ttttgctgct tttcccagaa ttaccaacgc tgtgggtgag
    26761 agtcattttt gccgtcatca cttacggtct tttacaatct tttgtcttgt atgctgtcga
    26821 gtaccgaaag atgtttcaaa gaaaataaaa aaggggtgtg acctatgtcg cattcctttt
    26881 tttgataaat agactcccag tagctacttc tcgataataa gccaaccaaa ccaaaaacag
    26941 gagaagttgt ttttgaaatt atttcttgtg attcggagtt aaatggtagg ctcacaacct
    27001 cttggtaaac ggtgttcaat cagctgacct tcggtattag ctcaaaaaac ggcaaaatat
    27061 gagcagctat ttttccgttt gtttctctaa tactcaggtc aaaccgctga ttttacaacc
    27121 tcttccttaa acggtattca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
    27181 tgtagaaatc cattttcaca aaatttttct taatactcga aactaagcga ctttttcaca
    27241 atctcttcct taaacggtgt tcaatcagct gaccttcggt attagctcaa aaaacggcaa
    27301 aatatgagga gctattttcc cgtttttttc tctaatactt aggtcaaacc gctg
//
LOCUS       DACCQI010000027        27147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.027, whole genome shotgun sequence.
ACCESSION   DACCQI010000027 DACCQI010000000
VERSION     DACCQI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.027"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            29..3418
                     /locus_tag="IVO76_000906"
     CDS             29..3418
                     /locus_tag="IVO76_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7428887.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            3421..4842
                     /locus_tag="IVO76_000907"
     CDS             3421..4842
                     /locus_tag="IVO76_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7428888.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            4839..6716
                     /locus_tag="IVO76_000908"
     CDS             4839..6716
                     /locus_tag="IVO76_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7428889.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            6808..7650
                     /locus_tag="IVO76_000909"
     CDS             6808..7650
                     /locus_tag="IVO76_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7428890.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(7802..8677)
                     /locus_tag="IVO76_000910"
     CDS             complement(7802..8677)
                     /locus_tag="IVO76_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7428891.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            8884..9915
                     /locus_tag="IVO76_000911"
     CDS             8884..9915
                     /locus_tag="IVO76_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7428892.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10004..10309
                     /locus_tag="IVO76_000912"
     CDS             10004..10309
                     /locus_tag="IVO76_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7428893.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            10488..12503
                     /locus_tag="IVO76_000913"
     CDS             10488..12503
                     /locus_tag="IVO76_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7428894.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            12505..12789
                     /locus_tag="IVO76_000914"
     CDS             12505..12789
                     /locus_tag="IVO76_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7428895.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            12815..14188
                     /locus_tag="IVO76_000915"
     CDS             12815..14188
                     /locus_tag="IVO76_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7428896.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(14271..16286)
                     /locus_tag="IVO76_000916"
     CDS             complement(14271..16286)
                     /locus_tag="IVO76_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7428897.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16308..17522)
                     /locus_tag="IVO76_000917"
     CDS             complement(16308..17522)
                     /locus_tag="IVO76_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7428898.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            17743..18687
                     /locus_tag="IVO76_000918"
     CDS             17743..18687
                     /locus_tag="IVO76_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7428899.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            18983..19927
                     /locus_tag="IVO76_000919"
     CDS             18983..19927
                     /locus_tag="IVO76_000919"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7428900.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(20594..20992)
                     /locus_tag="IVO76_000920"
     CDS             complement(20594..20992)
                     /locus_tag="IVO76_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7428901.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(20989..21225)
                     /locus_tag="IVO76_000921"
     CDS             complement(20989..21225)
                     /locus_tag="IVO76_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7428902.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            complement(21385..21702)
                     /locus_tag="IVO76_000922"
     CDS             complement(21385..21702)
                     /locus_tag="IVO76_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428903.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            complement(21840..24068)
                     /gene="topB"
                     /locus_tag="IVO76_000923"
     CDS             complement(21840..24068)
                     /gene="topB"
                     /locus_tag="IVO76_000923"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7428904.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            complement(24242..24652)
                     /locus_tag="IVO76_000924"
     CDS             complement(24242..24652)
                     /locus_tag="IVO76_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7428905.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            complement(24769..25932)
                     /locus_tag="IVO76_000925"
     CDS             complement(24769..25932)
                     /locus_tag="IVO76_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7428906.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            complement(26110..26964)
                     /gene="aroE"
                     /locus_tag="IVO76_000926"
     CDS             complement(26110..26964)
                     /gene="aroE"
                     /locus_tag="IVO76_000926"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7428907.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
BASE COUNT         8806 a         5145 c         4977 g         8219 t
ORIGIN      
        1 acagaaaaaa agaaaaagga ggggaaaaat gacgaccaca gggaagaaac tgaaagttat
       61 tttcatgctg ataatattga gtttatcaaa ctttgtgcca ttatctgcaa tagcagacac
      121 tacagatgat ccaacagttt tagaaacaat ttcagctgaa gtcatttcgg atcagtctgg
      181 aaaaaaagca ctgaacatca agctaaatgc gaataacacc agtgctgaaa agatagaaaa
      241 agaaattggt ctagtcgaaa attacttaag tgatgtggaa agaaaagaag gagatggcta
      301 tgcttatcag gtaaatagcg ggaaaattac gttggaaatc tcatcaaaca ctaaacaaac
      361 tatcgatctg agttttccaa tcgatccagc actttaccac agccaggcaa acaagctgat
      421 cgtcgataat aaagaatatg acattattga tgagacagaa aataagaaag atacagatgt
      481 gtcagtacca aagccagacg aaatagaaga agaatcatca aaagaaaacg aaaattctgt
      541 cagcccattt acattgccta cattatcctt gccagctgtg agtgtgccat ctaatcaaac
      601 gattcctaca gaatatacaa cagatgatca gggcacttat cctaaagcca gttggcaacc
      661 tacaggaaat acaaatgttc ttgatcatca aggcaataaa aacggaacaa atcaatggga
      721 tggtataaat tcttggaatg gagatcctaa tgatcggacc cattcgtata tcgaatatgg
      781 aggaaccggt aatcaagcag actatgcgat acgaaagtat gcaaaggaaa caagtacacc
      841 cggattgttt gatgtttatt tgaatgctcg tggaaatgta caaaaagata tcacgcctct
      901 tgatctcgta ttggtcgtag actggtcagg aagtatgaac gacaataatc ggatcggtga
      961 agtaaagatt ggtgtcgatc gttttgtcga tactttagca gatagcggta tcacagacaa
     1021 aatcaatatg ggatatgtcg gctactcaag cgaaggatat agctacagta acggtgcagt
     1081 acagatgggt tcatttgatt cagtgaaaaa tcaagtaaaa tccattacac cttcacggac
     1141 aaatggtggt acttttacac aaaaagcact aagagatgca ggaagcatgc tatccgttcc
     1201 aaatggacat aaaaaagtga tcgttttgct gacggatggt gtaccaacat tttcctataa
     1261 agtacagcgg gtacacgcac aatcaagcag caattattac ggaactcagt tttctaatac
     1321 gcaagatcgg ccgggaaata cttctctaat ctcaagaatc tatgatgcac ctgaccaaaa
     1381 caatctatcc agaagaatcg acagtacgtt tatcgcaacc atcggagaag cgatggcact
     1441 caaagaacga ggaatcgaaa tacatggtct tggcatccaa cttcaaagcg atccggcagc
     1501 tggtctctca aaagcagaag tagagtctcg tatgcgacaa atggtttcat cagatgaaaa
     1561 aggcgatctt tactatgaat cagctgatca tgcaacagat atctctgaat acctagccaa
     1621 aaaagctgta cagatctcag caactgtaag caatggacaa ataaatgatc caatcgcaga
     1681 accattcatt tatcagcctg gtacactttc agtcaagagt gtggggacaa gtcctacaac
     1741 ggtcactcca tctatttcca tagaaggaaa taccatcaag agcaatcaga tctatttagg
     1801 aaaagaccaa gaaatccaaa tccattacca agtgagaatc caaacagaaa atgaggactt
     1861 ccatccaaat ttctggtatc aaatgaacgg caggacaact ttccagccaa acattgatac
     1921 caatgaatta gctgaattcg gtataccatc tgctaaagct cccggagtca gtcttcacat
     1981 caaaaagtta tgggaagaat ttgacaacaa tctagctgat cgtccagatc aagttacttt
     2041 tgagattcaa cgggaacata cgacaaatgc tgcagcttgg aaaaacggat atattcgaat
     2101 cattaaacca gctaaagata caacaaatac gtgggaacgt gcagacattg acaaattatc
     2161 tgcaaatagc ggagaaagtt atcaagagat attatcacta cctcaataca ataatcaagg
     2221 tcaagcattc agttaccaaa caatcaaaga attacctgta ccaggatacg attctcaaca
     2281 aatagatgca atgacatgga aaaatactaa acaattcaca ccgttaaact tgaaaataac
     2341 gaaaaattcc tctacaggtg aaaaggatct tattggcgct gttttcaaat taacaggaga
     2401 ttctattgat actttactaa cagatcatgg cgacggaacc tattctcttc cagaaaatgt
     2461 caaattgcaa aaagaaatga cctatacgct gacagaaaca aaagctccag aagggcatgg
     2521 attaagcaaa aagactactt gggaaatcaa gatcgcttct gatggtacgg taaccattga
     2581 tggaaaaaca gtcactactt ccgatgatac gatccagttg actattgaaa atccttttgt
     2641 tgaagttcct gtagcagtac gtaagtatgc gatgcaaggg acggacaaag agataaatct
     2701 taaaggagca gcattttccc tacagaaaaa agaagcaaat ggtacttatc agccaattga
     2761 cagccaaaca acgaatgaaa aaggtcttgc cagttttgat tcactcacac ctggtaaata
     2821 tcgagtcgtt gaaacagctg gtcctgccgg atatgatact tcgccgggaa attatgaatt
     2881 ccaaatcgat aaatatggaa aaatcattta cacgggaaaa aataccgaga tgacaaataa
     2941 tgtatggacg ctcactcatc aaaatcgact aaaagcgttt gatctaacgg tacacaaaaa
     3001 agaagacaac ggacagacat taaaaggagc aaaattcaga ctgcagggac cagaaatgga
     3061 cttagaatcg ccaaaagatg gacaagaaac agataccttt ctattcgaaa atttaaaacc
     3121 tggaacttat acgctgaccg aaacttttac accagaagga taccaaggtc taaaagagcc
     3181 agttactata gttatacacg aagatgggtc aattcaagtg gatggacaag atcatgaatc
     3241 tgttctgtca ccaggagcca aaaacaacca gatttcttta gacatcacga atcaggcaaa
     3301 agtaccatta cctgaaacgg gaggaattgg ccgtttagga atctatctag tagggatgat
     3361 tggttgtgcg ttttctattt ggtatctttt tttgaaaaaa gaaagagggg gcagctaaat
     3421 atgaaaaaac ttggttggct tagtatgtgt ctcttcttgt tactatttaa accagctttt
     3481 actcaggtag caacagaaac agaaacagaa atggttcaga ttactttaca caaattgctt
     3541 ttcccaaacg ggcaactgcc gaaaaatcat ccaaatgacg gacaagaaaa agctttatta
     3601 caaacgtatc gaggattaaa tggtgtcaca ttccaagttt atgatgtcac agattctttt
     3661 taccatctac gggaaaaggg caaaacggta gaagaagcac aagcagagat cgcaaaaaac
     3721 ggtgcgtctt ccggtatgtt taccgcagaa gcaacaacta caactcttaa caacgaagat
     3781 ggtatcgctt ctttttctct ggccgctaaa gatcaagaaa aaagagataa agcgtatctt
     3841 ttcattgaat ccaaagtacc agaagtcgtc aaagaaaagg cagagaatat ggtagttgtt
     3901 cttcctgtac atggacaaaa caatcaaaaa ctttcaacta tccatttgta tcctaaaaat
     3961 gaagaaaacg actaccctga tccacctttt gagaaggtat tagaagagcc tagaaatgat
     4021 tttacgattg gtgaaaaaat cacttattcc ttgcatacga caattcctgt aaatatcctt
     4081 gactatcaaa agttcgaatt gtcagatagt gcggatgaag cattaacgtt tttacctaat
     4141 agtttaacga tttcatcgaa tggagaaaag ctgacagaag gctttgtcat acacaagaaa
     4201 cctcacggat ttgatgtttt attttcgatc ccttcgttgg aaaaatatgc tggaaaaaaa
     4261 ctgaccattt cttatcagat gcagctaagc agtacagcac aggcgaacaa ggaaatcaac
     4321 aacaacggaa cactggattt tggttttggt gtcagtacaa agaaagtctc tgtatataca
     4381 gggagtaagc aatttgtcaa aatcgagaca aataaaccag ataaacgatt agctggcgca
     4441 gtattcctta ttaaaaacaa agcaggaaat tacctccagc aaacagccaa cggatacaag
     4501 tggacaaaga acgaatcaga tgcgcttcac ctgatttccg ataaaaatgg cgctttttca
     4561 atttccgggt tgaaaacagg aagttatcga ttaaaagaga tcgaagcacc ttctggttat
     4621 attttaagtg aaacagaaat tccgtttacc atttcaactt ttctttctga ggataaagag
     4681 gcggacagta tattgaaagt agtcaataaa aaagaaaata gccgtccatt tcttccaaaa
     4741 acaaacgaaa cgaaaaatac acttttaggc gttgttggta tggtattcgc aagctttgca
     4801 atctggttgt ttatcaaaaa aagaacagga gtgaaaaaat gaaaaatcat aaaaaaataa
     4861 acgttatgtt aggagtcctt ttccttattt taccattact cacaaacagc ttcggcgcaa
     4921 aaaaagtgtt tgcagaggag acagcagctc aagtcatcct tcataaaaag aaaatgactg
     4981 atttacccga tcctttaatc caaaacagcg ggaaagaaat gagcgaattc gatcaatacc
     5041 aaggattagc cgatatttca ttttcagttt ataacgtcac tcaagaattt tatgcgcaac
     5101 gagataaagg agcgtccgtg gatgcagcaa aacaagcagt ccagtctttg actcctggta
     5161 caccagttgc ttcaggaacg acagatgctg atggaaatgt cactttatct ttacctaaaa
     5221 aacaaaatgg gaaagatgca gtctacacga tcaaagaaga accaaaagac ggagtgtcag
     5281 ctgccgcaaa catggtttta gctttccctg tatatgagat gatcaaacaa gcagatggct
     5341 cttataaata cgggacagaa gaactagata ctatccatct ctaccctaaa aatacagtcg
     5401 gtaatgatgg aacgttgaaa gttacaaaaa tcggtactgc cgaaaacgaa gcactaaatg
     5461 gagcagaatt tattatttct aaagaagaag gaacaccaag cgtcaaaaaa tacatccaaa
     5521 gtgtcacaga tggattgtac acttggacaa ctgatcaaac caaagccaaa catttcatta
     5581 ctggtcattc ttatgacatc ggcaacaatg actttgccga ggcatctatt gaaaaaggcc
     5641 agttgatcgt taatcattta gaagttggaa aatataattt agaagaagta aaagctcctg
     5701 ataatgcgga aatgattgaa aagcaaacaa tcacgccttt tgagatcctg gcaaatagcc
     5761 aaacaccagt agaaaagacc atcaaaaatg atacgtctaa agttgataaa acaacacctc
     5821 aattgaatgg aaaagatgtc gcaatcggtg aaaaaattca atatgagatt tctgtcaata
     5881 tcccattagg tatcgctgat aaagaaggaa cgcaaaacaa gtacacaaca ttcaaactta
     5941 tcgatactca tgacgctgct ttaacatttg ataatgattc ttcaggaacg tatgcttatg
     6001 ccttatatga tggaaataaa gaaatcgacc cagtaaatta ttctgtcact gagcaaacag
     6061 acggattcac ggtttcagtt gatccgaatt atattccttc attaactcct ggcggtacat
     6121 tgaaattcgt ttactatatg catttgaacg aaaaagcaga tccaaccaaa ggattttcta
     6181 accaagcaaa tgtcgataac gggcatacaa atgatcaaac accaccgtca gtcgatgtcg
     6241 ttactggggg caaacgattt gttaaagtag atggtgacgt tacatcagac caaacacttg
     6301 ctggagcaga attcgtcgtt cgtgatcaag atagtgacac agcgaaatat ttatcgatcg
     6361 acccatccac aaaagccgtc agctgggtat cggcgaaaga atcagcaacg gtttttacaa
     6421 ccacaagtaa cggtttaatc gatgtgacag gtctaaaata tggcacgtac tatctggaag
     6481 aaacgaaagc gccagaaaaa tatgttccat taacaaaccg tgtagcattt actatcgatg
     6541 aacaatctta tgtaacagca ggacagttga tttctcctga aaaaatacca aataaacaca
     6601 aaggtacact tccttcaaca ggcggtaagg gaatctatgt gtatatcggt gcaggagtag
     6661 tccttctact gattgctgga ctgtactttg ctagacgcaa gcacagtcag atttagtctt
     6721 tttgcaagaa ttggaaaaag gagcagcgga ttacgcaaat gctccttttt cttttattat
     6781 caaacagtca tcacaaggag aacacaaatg aaaccaaaga aaaggaaact tctagatctg
     6841 ctgatgcttt tcttgcttct ctcaggaata gggatactag cttatccatt tgttagcgac
     6901 gcgctgaata attatctgga tcagcagatc atctctcatt atcagcaaca agcggtcaaa
     6961 gaaaatgaag aagtcatggc taaaatccaa gaaaacatga caaagaaaaa ccagcaatta
     7021 gcaaaagaag gtggaaatcc aggtgcagat ccctttacta agaaaaaaga acctgcgaaa
     7081 aaggatagaa cctattttga gaaacatacg atcggtattt tgacgatccc tgagatcaat
     7141 gtccatttgc caatttttga tgaaacgaat actgtgctgc tagaaaaagg tgcctcatta
     7201 ttggaaggaa cgtcttatcc tacgggggga gaaaatacac atgccgttat ttccagccat
     7261 cgaggactgc ctcaagcaaa gttgttcaca gacttaccga aactgacttt aggtgattct
     7321 ttctatatcg aaatcaatgg acggacactc gcttatcaag tcgatcagat caagacggta
     7381 gaaccgactg atacaaaaga tctgagaatc gaaaaagggc aggatttcgt gacactccta
     7441 acttgtacac cttatatggt caacagtcat cgcttacttg tcagagggca ccgggttcct
     7501 tatcatgcaa aagaagttca aaaagaagta cagaaagtat ccgagaacaa acgatttttc
     7561 ctgtatggat tgttagctgc atgtatactg attttcctcc ttcttatata tctttttcgt
     7621 caatcgttca aaataagaaa cagaagttag ctattcctca tttctataat atatctattc
     7681 gaatcgttcc gtaaacaaac aggctgaggt ttgaaaaaga aaacttcagc ctgtttttat
     7741 gatgatatca aagagagcct atcaaacact atctttctta ttactaaatg aggatctttt
     7801 cttaagtttt ccgatattct ttaagaaggc ctttttgcat caattcttgt ttgtttttca
     7861 agttgcggat attctcttca aaattttgag aatatatcac tgagaataac gtatttaata
     7921 cgtaatcgaa ggcaatttga gaggagaaag tcgctacttt agcaaaatca tattcctcct
     7981 gcgatataac aagacacata tcagcctgtt tggccatttc tgaatgctga tttccggtaa
     8041 tcagcaaggt aggggcctct acatgcttca aataggtcat tattcgttca taatgatgga
     8101 tgcgaccgct gtagctaata aaaatggcgc aatcctcgct agtcaaattg gctgcattcc
     8161 aagaagaatc actgtattct tcagccatga tcaaaaactt attcaacttg accattttat
     8221 tctggaattt ccttgccgtg atttgagaat caccttttgc aaaaagaaag attcgctgtg
     8281 ccttgttcaa tactttaccg atttgatcta aatcttcatg ttcaatctgc aattgtgctt
     8341 tttttattga ctggatcgat aaatccgcca ttttctttgc aatttgctga gtgctatctt
     8401 cacttgaaaa aggaaaatta ggatcaatcg tttgattgaa ctgagcaatc aattgatgaa
     8461 cttcccgccc cagctcgaaa cggaattctt taaatccgct gaatcccatt tttttgcata
     8521 aacgaatgat agctgaatgg gaagtatacg tagcttttgc taattcttca atcgtcatgt
     8581 agactgcagc ttcgatattc ctacggatgt aatcgacaat ccgtttctct gtcgtggtaa
     8641 acaactcttg ttttgttagt ttttcttcaa taagcatgat ctgacctcca aattctatct
     8701 tactgataaa cacttggttc cgctactatc tttttgattt ttatccttac tttgaacatt
     8761 aatttcacca aaccgaatga taacaagtgt ttttctgaac ttttttacca gaatagctat
     8821 tataaatact ttctgcgctt ttctaggata tactgaaaac atcaataaaa ggaggcacca
     8881 ctcatgctta caatcgctta tcttggcaat ggaaagagta caaatcgcta tcatttgcct
     8941 ttttcaagaa aacttacgaa caaaatcaat atcaaaaaaa ttttttcccc atctgaacca
     9001 gtgtggaaaa cattcgaaga catcgaatat actgatcagc tgtctgatct ttgggatgat
     9061 cctgagatcc aattagttgt catcactaca cctagtcaat ttcattatga atatggaaaa
     9121 atggcactcg aacatggtaa gaatgtacta gtagagaaac cttttgcaga aacgtcaaag
     9181 gaagcagaag agttatttgc attagcaaaa gaaaaaggtc tatttatcca atgttaccaa
     9241 aacagacggt tcgattctga cttcttgaca gtccaaaaag taatcgaaag cggaaaatta
     9301 ggggatattt tagaagtaga aatgcattat gattatttca gaccggaagt accagaaaat
     9361 acgcatgaat tttctactgc gaacagctat ttatatggac atgcttgtca tacagtagat
     9421 caagttcttt cttatttcgg cgaaccagat gatatcagct atgatgtacg tcaactttta
     9481 gggaaaggac gaatgaatga ttacttcgat ctggattttt tctatggaaa tatgaaaatt
     9541 tcgataaaat ccagttattt ccgcatcaaa gaacgtccaa gtttcgtcgt ttacggaaaa
     9601 aaagggatgt tcgtgaaaca aacaaaagat cgacaagaag aacatctgaa aatgttttac
     9661 atgcctgatc atgaagactt tggtatcgat ttgcccgaac attatggtgt attgacctat
     9721 atagatgaac aagggagtta tcatgaagaa aaagtaattt ccgaggttgg tgattacagc
     9781 cgtgtctacg aagggattta cgagacatta gttaatggcg cagaaaaagt catcaaagat
     9841 gaagaaacaa tcttacaaat gaaaattttg gaagaaggaa tcaaaagcat tcaagaatca
     9901 gaaggaaaaa tatgacatat ttgtaagaat cgggtttgtt ttttgacgca taaactgtct
     9961 tattgtacga tgaaaaagta tagaactact aggaggaacc aacatgacaa attatgacgg
    10021 tgaaaaacac aaacgattaa gaggttcaat ggcacctaaa acagaagaag aagtggcttt
    10081 agcaggaact aattctggtg aaacagaaga caaagaagaa tcattagctg gcacaaactc
    10141 tggtgaaaca gaaaataaag aaacagcttt atctggttca aattctgtag aagaggaaac
    10201 aaagcctaat gagcccaatc actttgaaaa agatatcgaa aaagcggttg aagatgtcaa
    10261 agaaaaattt gaagaactga cctcaaaaaa taaacatgat catcattgat cggacgaaat
    10321 caattcataa aagcaggaga catttggtca cctgcttttt ttctgtatcc attcgataga
    10381 aatcgttcgg tacagtcgta aaaaaaatcc aactgtaagt tgtaccaaac acccctcgat
    10441 ttaaaagcgc taccatacta gaatataagt aggaggtgaa cagcgctgtg aactacaaaa
    10501 ttagttatct cctgaaaaca ccaagagaaa tcagtctaga aaaagcagca gagatcatcg
    10561 aagtaaatac aagcgaggcg atcaaacaat taactccttt tgccgaatat gtggatgata
    10621 cccgtatccg tataaagaaa ttccgagcaa tcgactgggc agaaaaattt ttggatatta
    10681 ctcgatttga agctgtgtat accgaacaag agcgaagaga tcttctttat ttgatggttt
    10741 attcgactgc aacgtctgtt tctgtttttc attttcaaga ttttcttcag gtttctaaag
    10801 ggacagtcct gtcagatatc aaaaagataa gaaatgaatt agaagtggaa gattgtcagc
    10861 tgtcgtatac taggaaaact ggttttaggc tgctagggtc agaagcaata ttaagaagag
    10921 aagcaaaaaa tcgcactgct cgtttgatgc agtcacttgc tggaagattt gggctcacgt
    10981 atttaaccgc aaccctttcc attgattatt acgccaaagt tagagatttg ctagataaaa
    11041 cgatttcact atcagatctt cgattcgcac caaatcgaat ggatgaatta gcttattatt
    11101 tgagtttgac aaccattcga attggaaaaa catccatgga actggtaaaa gatgctgaac
    11161 tgttggcttc tcttgcagtc actgacatta gtcgacaagt attgactggc cttggctatt
    11221 ccgaacatca aaattcggag attatatttt tctctctttg cttagcatcg atcgttgaag
    11281 gagatgtcca ggagcctgca cttgattttt tgctggaatg ttcttcagaa gtcatccatc
    11341 gcatggaatt cctgatggcc gtagaatttg cttcgtttcg tgaattattg atgaatgtct
    11401 tttatcactt ggtccctgcc tattttcgta tttcctattc tttttattta cctaatgtga
    11461 tgatcgatca aattaagcat caatatgctt ccatctacga aatgacaaga aaagcattac
    11521 ttccgttaga aagacgaata gggaagagca tccctgaaga agagattggt tttttcacta
    11581 tactatttgg cggagagatc agaaaagcgg acgcagaaga aagaaataga aaaataagag
    11641 ccgtgatcgt ctgtccaagt ggcattagct cttcattgat cttaaaaagt gaacttcagc
    11701 aattattccc gatgatcttt tttacagaga ctaattcagc tgaccgctta cacgaggttg
    11761 acgaaacttc ttacgatatt attttttcta ctgtacctct cacacttact gataagaaaa
    11821 agtctctcta tgttctgcat ccaatcatga ctgacttgga aaaaaatcag ctgatcaatc
    11881 aagtacaaag agattggctg ataccaggat tttcaatgcc ttctgcccaa gaattgttgt
    11941 cggcgctact gccttatatc gaattaaaag aaggcgtaga cgaagaaaag ctctatcaag
    12001 tattaaatcg taaaatgaac aaacttttag aaaagaagga ggatcaacgt cctatgctat
    12061 ctgaattatt aacagaagaa atgatccaat tatcggatca accaaaaaac tggcaagaag
    12121 caatcacatt tgccgcacag ccgctattat cagaaaataa aatagaagaa agttatatca
    12181 aagctatgat cgagcgcgtg gagcagtatg gtgcgtttat ccatattggc gatcacatcg
    12241 cattacctca tgctcgtcca gaagatggcg taaacgaagt agggatgtct ttgctgaaac
    12301 ttcaacaacc tgtggatctg cttgataatc cagcccatcc cattagtttg ttcatctgtt
    12361 tagctgctat cgataatgag gcacatcttc gggctttggc aaatttaaca aagctacttt
    12421 ctaataaaca aaatcttcaa gatttattac acgcatcaac aaaagaagaa atattagcga
    12481 tcataagaaa aggagacgaa tgatatgaaa atttttgcag tatgtcaaag tggattagga
    12541 acaagtttta tggtagagat gaacatcaaa caagcacttg cagatcttgg agcagatgtg
    12601 gacgagtttg aagtaagtca tacggatgtt ggaagtgtat ctagcgatat ggctgattat
    12661 ttcttcattg agcattcttt gaaagatacg ctaacttcta tacctgatga aaagctcgta
    12721 cctttgcaat cgatcattga cagcgaagaa gtaaaagaaa aagtaacgaa gatcttgtca
    12781 aaagaataaa aatgattcaa gggaggaact atcaatggat aaattgttga atttgtttgt
    12841 gtcaatcgtc agtcagccct ccatcctcgt ttctttgatt gcactgcttg gtctcgttct
    12901 tcagaagaag aaattttcag aagtgatcca aggaactatc aaaacatttg tcggattcct
    12961 agtactgatc ggcggtgctg gtttaatcag taattcacta accccttttg cttcgatgtt
    13021 ccaaacagct ctgcatactc aaggagtagt tcctagcaat gaagcagtag tggcgattgc
    13081 tttgcaacaa tacggtacac ctactgcttt gatcatgcta gtagggatga tcgtgaatat
    13141 tttacttgct agattcacaa gatataaatt catttttctt acaggtcaag caatgcttta
    13201 tgtttcttgt ctaacagcag tcattttagt aagcgcgggg tttactgcaa atttgtggat
    13261 gatcttatta ggcggggtct tcgaaggaac actattaact attacgcctg ctctttgtca
    13321 accgtttatg aagaacatca ctggaaacaa tcaagttgcg ttagggcata ccggaaatat
    13381 cggttatgct tctagtggat ggatcggaaa gttttttgga aacaaagacc gttctacaga
    13441 ggacataaag attcctaaaa gtttcagctt tttgcgagat tctactgtat cgatcatgct
    13501 cttgatgtcg atcgtatatg tcgcacttgc tttgttagct ggtcctggat ttgttgaaag
    13561 agaactaagc aatggagaca acgctgtaat ctttgcactg atccaagctg gaacattcac
    13621 agctgggttt gttatcgtcc ttcaaggggt tcgaatggtg ttggcagaaa tcgttccagc
    13681 attccaaggg attgctaaaa aactcgtacc cgattctaaa ccagcattag atgttcctat
    13741 cgtttttcca tatgcaccta atgctgtatt aattggcttt tttgttagct tctttgtagg
    13801 aacactatcc atgtttgcta tgataggctt gcaaactaca gttatcattc ctggggtagt
    13861 aggacatttc ttctgcggag ctgcttctgg tgtcttcggc aactcgactg gcggtagaag
    13921 aggggcggta ttaggttctg ctttcaatag cttattgatt agttggctgc cattatttat
    13981 ccttccagta ttaggagatc ttaaaatcgc ttcttctaca tttgccgata ctgattatct
    14041 agtaccagga cttgtacttg ggaaactagg agaatacggt caaatagcct tgacagttgg
    14101 aatcatcgct ttctttttac ttgtcgtatt atctagcttt ctttttgccg gaaaaagaac
    14161 aaacattgtt tctaaagaac agaattaagc aaacttcaaa ataaaaattg cttggacaat
    14221 atcagaagaa gatttaaagt cttctgatat gtcgcaagca gttttttgtt ttatgcattt
    14281 atttttgaat agcttgttaa gacaagtggc tgttttgcta gaagaggata acttttacca
    14341 ttcattttca ctgttccgcc tgttctatca gaagtaatca cgatcttatc ttttgttttt
    14401 tcaagcgaaa tctttccatc tttgataggt agcgtacaat tcagtgtttc aaatgaatct
    14461 agattaggtt gtatcataaa ttcttcataa cctggctttg ttggatacaa accgataaaa
    14521 tagcgtccta aaagatagat tgggcttgct ccccatgcat ggcataagct ttttccataa
    14581 ggatctccat acatactgta cttttcattt cctgtttgag tagggtcgta ttcttcccag
    14641 aaagttaccg catcatgaga taacatccct ccccaataat caagaatcgt ttggtagacg
    14701 atattcgtct gtccgagttt gcataaagca tcttgttcat agaatttgaa atatggagta
    14761 gtaatctgtg tgatagtgtc attcaataag acattcttta agatcgattg ctgttggtct
    14821 ttgttagcaa aatcgaataa aacagcgaaa atattcgcat gacgcgtcac atttcttctc
    14881 ccagattcat agcaatcgat aaaagcgcca agttcttcat cccaaaagta gtcaaatagg
    14941 ttttgtttca atagttgata tttttcttga tagagatgag tatgataatt ttcgttctct
    15001 ccatatgttt cagctaagat atctccgcaa gtgatcatgg aatgataggc tttcagtaat
    15061 aagacttgtt ctgcagcaag cgttccttct ttatccattt cagaccaatc aataaatatc
    15121 caatcttttg ctctcccata aataaaccct aattcattcg tttgctccat caagtatctc
    15181 attaatgatt gcatcttagg gtagacgatg cgtaaaaatt catagtcttt cgtcatcata
    15241 tagtggtttt cgataccaat caaccacaag atcgaatagt caacgatagt attcatatgc
    15301 tgcttgattt cattttgtcc tcttaatgct aatatcgttc gtttattgat ttcctcgtca
    15361 aaaaaaagat attgattgat catgaatgat tgatacgcat ctccagacca aatccagcga
    15421 tctcgtttga taccgtcgat aaaaaacaaa tcactacata acttgaaggc ttcttctgat
    15481 actgaccaga tctgattcag taaagaagaa tcgcttgaaa aagcagcttg tttcgaaaga
    15541 gataaaaatt catgaaaaac ttgaagttga accattccag tagaaacctc tggtataaac
    15601 aaatagcgaa aagcgcgttt acgagctttc atttttcttg tcattcctat ttgcttatag
    15661 tagcaataat cgatatccat agcttctaca tcggactctc cataacatag agtgatagat
    15721 tcgatatggt catccagaga tattagttct acacagccat tcactgctcg cccaaaatcg
    15781 aataagattc ctccgccaat ctccgtttca gtgactggat ggacgcattc tttttgataa
    15841 tagatttgat ttggatcttt tgcgatatcg acataaagcg gactccagcc agctggatat
    15901 tcttcaataa aattacttgc tgtccagcca atatctgatt ttacttcgtc tccctcgata
    15961 aacatcgctg gtagccctga tgtatgtcct acaaagatac gaatttttgc tttaccagct
    16021 ggacaagtca gccattttcc acatggatgt ttttgtccat taatttctac atacccaact
    16081 ccttggatcg tcactttaaa catggaaggt tgatcaagaa agtaatatct cttaaacttg
    16141 acgttccgat ggcaatcatc catgtaccag tacgctggcc agtcaaaacc tcgttcttcc
    16201 cgttggaaat tttgtagcag tccatggcgg atctcaaagt ctccaggata ccaaagccat
    16261 ctatctttat gtttttcatt tgtcatttta cacgacctcc atcaaattta ttttgttgtc
    16321 aattgttctt ttatttcttc atctactctt atcactgtct ttctattcgt cctgtgtact
    16381 actagaaggc taataagcgc gctaatcaac agcaagatac tgccattgac gaaaacagca
    16441 agtgttttac tcccaagaga agcaaaagag agaaagagca aaccatatgg tgcaatgaaa
    16501 gtacctaaat tagcgccaac caataagatt gaagtattga aactctccgc gtacttgggc
    16561 gcttggatac ttacttggtt gaataagtaa ggaatgaata acgaaaaaga aattcctgtt
    16621 aggatcgcac caaacccaat cacatacagg tttatcacag tggcaatcaa tacaaagccg
    16681 attcccatca gtagtaaaga aagtgtaagc atatagcgat gtgcttttgt ctgaagccag
    16741 ccaaaacaaa ctccggcagc catacttgcc aattgcatga ttgttaagat attcgctgca
    16801 ttagtttcgt ttcctagaga ataagtcgtc aggactgtgg ctaatttaat cgatacagca
    16861 ttatatacca cgatgaccag caagagaaaa ataccataac taatcatttt aggattcgtt
    16921 ttgattactt gtcttgttgg ggctgtgcca tgcgattgaa gttcagttgt ttcaggtgta
    16981 ggaacaaaca aaacaaacaa gacaagaata ggtaagctga tggcgtagac taaaaaggta
    17041 tattgccaac cgaactgtac tagctgtcct gcaacaaatg tcattaaagc tgcaccaaat
    17101 ccttgaaatg cactttgaaa accaatcata gaggctcgtt catctccgtg ataaaaggca
    17161 gcaatcatgc tgactgccaa agaattgatc aatccaaagc caatacctag tccagctctt
    17221 gaagctaaga caatactgta actttgtgag aagaggggga ggataccaca aatcgatgca
    17281 atcactaatc cgatgatcac agtttgtttt tgtccgattt tcctagcaat aaaactacta
    17341 atcaaaacaa aaatagtgac cataaaagag ggagcagtcg ttaataattc aacagcagat
    17401 aaagaacgcg tggcataagt agctgacata ctgggaattg cagacgtgac tgcaccagca
    17461 gaagtcaaaa taagagagac tgacagcaga gaaaatttca tcatcttttt attcataagc
    17521 atgttccttt catttttgta agcgcttatc aaaaatatag tatgaagtgg ttattctctc
    17581 aatagcggaa tcacaggaag ttcatgtccc aaccatagaa agtctttatg aaaccaaaaa
    17641 tagctttgca tataatagca gtacagcgtg aactaataag cgaaagaaac cgcttacaaa
    17701 aaggagggag tcaattggaa ctagctgaat tagacatttt ttttgcgtaa cgaagaatca
    17761 atcgaaactc tgcagcgtga acaaggaatg aatatcaatg atttaaactt agcttaccat
    17821 cttcagacac cgccaaaaat ccaccgaggg gatctatttc aacagggaaa tattcatatc
    17881 agtaaacacc gtcgatatgc ggaagttccc gagcatacac atgattttgt tgaaatcaat
    17941 tatatgtata gcggtacatg tttacaaatt attaatggag aaaggataga gatggaagaa
    18001 agtgacgtgg tgatcattga taaagaagct actcaaagaa tcgaatacac agggcaagag
    18061 gatattttaa tcaacatctt attaaaagaa gaaaccttat ctacagagat attgaatcat
    18121 ttggcaagca caaataatct actttcttct tttttgataa atgcatcaaa agaaaaaggg
    18181 gaacatacac aattcattca ttttcgcggc ggaaaaacaa gtcgtttgac gcatttgatt
    18241 gaaaatatgc tgttgattta ttttgataca tacagtcata agatccgttc gttaaatctc
    18301 tatacatcac tattactgat cgaattgact catttattag aacaacaaag cttttcatca
    18361 gcagatggat gcgcagaaaa tgagatgctt agagtattag attatatcga taagcatttt
    18421 acttcttgta cgttaaaaga aacagcagct cattttggct acaatccggt ctatcttagt
    18481 aataaattga aaatgtattt tgatgcttcc tttcaagaac tggttctaaa gaaaaaattc
    18541 gactttgcgt tggaactgat tcaagaaacg agtttttcgt tggaagaaat cgctaatcgt
    18601 atcggatata aacaaacgac ttctttattc aaattattca agaagtattc aacatgtact
    18661 cccaatgaat ggagaaataa acattaagaa gaaaatcttt cactaaagca tatgaaggaa
    18721 aattgtttta ggtgcgattg ctgtcttatt atatcgttgc agcgaatttt tttggattta
    18781 ttttcagttc taaacttttg gttgtattat caggggatat aggtgtaaac atttgtcatt
    18841 tatctgttat gcgtattcag gaattcgttt ccatcaaaaa ttataataac aactattata
    18901 attaacatgt tgataaaatg aataaacaag actatacttt tcatgaaagt aacaaatagt
    18961 tatgaaaagg agctgtttat tcatgaaaaa aacaagtcgt aaagtagtaa tcgtcggtac
    19021 aggttttgtt ggaacaagta tcgcttatgc aatgatcaac caaggaatct ctaatgaatt
    19081 agtattgatc gatgtgaatc aagaaaaagc agaaggggaa gcattggacc ttttagatgg
    19141 tatggcttgg ggcgatgaaa atgtggctgt atggtcaggt ggctatgaag aatgtaaaga
    19201 tgctgatatc gttgttatca cagctggtat caaccaaaaa cctggtcaat ctcgtttaga
    19261 tttagttaaa acaaatgcgt caatcatgcg ccaaatcgta aaagaaatca tgggatcagg
    19321 attcgacggt attatcgttg ttgcttcaaa cccagtagat attttgacat atatcgcttg
    19381 gaacgaatct ggtctgccaa catcacgtgt tatcggtact ggaacaacat tggatacaac
    19441 tcgtttccgt aaagaaatcg cattgaaatt aaaagttgac ccacgcagcg tccacggcta
    19501 tatcttaggt gaacatggtg attcagaagt tgctgcatgg tcacatacaa ctgtcggtgg
    19561 taaaccagtg tttgaaattg tagaaaaaga ccatcgtatt gcccaagatg aattagatgt
    19621 gattgctgat aaagtccgta atgctgctta tgagatcatt gatcgtaaaa aagcaactta
    19681 ctatggtatt ggtatgagta ctgcacgtat cgtaaaagct atcttgaata acgaacaagc
    19741 tgtattgcct gtatctgctt atttaactgg tgaatacgac gaaaaagata tctttacagg
    19801 tgtgccatca atcgttgacg aaaatggtgt tcgtgaagtt gttgaattat caatcaacga
    19861 agaagaaaaa gcaatgttca gcaaatcaac aagtgcttta agagaagtat tgaacactgt
    19921 attataagaa acaatgaata tgaactaaaa atcctaaaac tgcttaacag ctgaaatagc
    19981 tgttaagcag ttttttattg atatctaata ggtaagcaaa taaacagaat ggttaaaatt
    20041 aatcggtccg aaaatgtatc cgtacatatg aacatatgaa atttaatctg ttgattacaa
    20101 taagcgaatc gttatcaatg tttatctgtg gttaattaaa aaatccttcc gcaacggcaa
    20161 atacaatgat tcatatctaa ttcgttcata tttgattaac tgaataaaga aactggtttt
    20221 tatcattcaa ttaaacgaca aaaaaagtaa gaaaaactgc aaaatttttt tataattttg
    20281 cataagcaga ttttgatgga aaaatgacga tttttgataa aaaaccgttc gagagaggtt
    20341 tatcattttt taaatctact ttgtataata tatattatgt aaactaaaat atataaaaaa
    20401 tcagcccact agttctaaac cactatttcc tactattcac tcttttgtat tccttttaaa
    20461 tcaattattt caaaattaat ccatattttc catattttta tgaaaaaact attttgtatg
    20521 attaaacata aaaaaatcag catattcctt aatgacatgc tgatctaatt gagaaataaa
    20581 gtttttaatt cgttcatttt attgcggttt gtcgtaaata actggttatc ttggacttta
    20641 gtgaatcgaa atccaatgaa ctagtggtaa tcattatgat aaaatcaaca gcttcttgac
    20701 gagaaaaagc aactggacaa ttattcaatt gaagaaacaa caacatggca acaagagcag
    20761 ttcgtttatt gccattatga aaaggatgtt ttttagctaa attaattgct aaaatcgccg
    20821 ctttttcata gatttcagga tagagttctt gattgaatac tgcttgtgaa ggctgtttga
    20881 cggccatatc taatgcattt gcatctttta caccgatcat ttcgcctggc gagtgacgta
    20941 aaataacttt cgcgttgatt ttgataattt ctttagtcga aagatacctc ataaatcaac
    21001 caaatctttt aacgcgtctt catattgatt catgatcgaa tcaactaatg aatcaatatc
    21061 aggttcttct tgtgctttga tcattcgtac agaaccatca gattgaaaaa cataactgat
    21121 tgcgtcacct gtctgcacac ccatgaattc ttttacttct ggtgggatag ttgttacatc
    21181 gctattgcca gattttctaa ttttatactt tttcattttt ttcatatttc gccaccctct
    21241 ataccattct cattctatag taagtacatt atatccaagt atatactttt aatcaatgta
    21301 tatactttta ttttgctttt tcttacgaaa aaatgacaaa gaaaaaagtt tgtgacatga
    21361 cttttgtcac aaactctcgt attcttaacc aaaatattga tgaaatactt gtgtaataac
    21421 tggtagaaaa taaataatga tcatagccgc taccaccacc aacaattgtt ttcgctgttt
    21481 ctttcgcttt agcatgcttg tcgttttatg aaaaaaataa gtagcgcttt tagctcctgc
    21541 agtcaaagtc gataaaatca acgttggtga atatgctgtt tgattgtgcg tcaatgacaa
    21601 caacttcgtc ccgtaatcag gatactttag aaatgcttgg tacacagcca caatcgtgat
    21661 tgctcccata agataattta aaaatgaatg cacttttttc aaataaaaaa actccttgct
    21721 atctatataa aatcagccta ccccaacgtt tttgcttgat tcactgatat tttttagttt
    21781 tattggtatt tatattctca cttttcggaa taaatattcc gctatttcat tgttttcatt
    21841 tactctactt cgttttcatt attttttctt gatgtttctt tgtttccatc aactgctttt
    21901 gttccactgt tttcgatcca tggaaaagaa aaaccgattg tcatatcttc ttttaatact
    21961 aaaactgcat cgaatttccc ttttttacca gtaaaacctt tgacaacttt acttgtcttt
    22021 ccttttgaaa gcaaatctct gagattcgtt ttagtcagct tctttttagc tactgttggc
    22081 cacaatttga agtcgcattc cggattcaag catgtagcga ttttaggata aagcatgact
    22141 attccctttt tacattttgg acatcttcct aattgatcgt ttttcttctc catcgctttt
    22201 atctgacgtg tcccgctaat ctcatctgaa aagtctacct tgtcgatttg attaggaact
    22261 tctgccaata aatgtaggat gaattttttg atattctcta aaaaaacaga tggtgtcccc
    22321 tcttgtttac caatttttct caagtaccct tcccagcgcg cagtcatttc tgcgctcgtc
    22381 agtaatggtt cattggctac tgccttacaa agagtgattc cttttggcgt aacagttatt
    22441 tcgtttttat tgacttcgat gtaatgcttc tgcttaagat tttcaatgat attggctcgt
    22501 gtagcttctg tccctattcc ttctacttct ttgagaattt cttgcgcttc atcattatcc
    22561 agcgtttttc ccgcagtttt cattgctgtg atcaacgttc cttcggtata gggttttgga
    22621 ggttgtgttt ctttctccat acttttcagt tctacttctg cttcttcatt ttctacgata
    22681 ggaggaagag tctgagcttc tttctttttt gacttgatgc gtaagatttc ctgccaacct
    22741 tgctgtaatg ggatctttcc gatgcttttt agctttaatt gctgtacgcc cgtcattacg
    22801 gtcgtctctt cgtagatata atctgcagca aacatagcca cagtagtgcg caaaacttgc
    22861 aaatagattg cttgctgtaa tttggaaagc ttcgcaaagc gtgcaggagt cggaatcgtc
    22921 tttgtcggaa taattgcatg atgttcttga acttttttcg catcgacata tcgtttcttt
    22981 gctttgatct cagttgtctg tacttcttct attcctaaaa attttttata attttgcaga
    23041 tgtttaacca gatactgatg ctcattttct gtaatgtaaa aggaatccgt tcttggatag
    23101 cttagaaact tgctttcgta caatccttga gcagcatcaa gtgtagcttt ggcacttgct
    23161 ttcattagct gattcatctt tgtctgcaaa ctagatagcg aaaataaacg tggactatgc
    23221 aacctctttt cctttttcgt aacttcaaga atccgtccgt tttgcttacc taaatgcgct
    23281 ccaagttttt ctatttcctt cgttaactct tcttgggttt taaatgcttt ttttggtacg
    23341 atttttccag aaaactgccc tttgtttgtc gtaattaaac cttctccttc aaagtacgtt
    23401 tcttttttga agtgtttgat ttcttcttgc agctgataaa tcatatagag tgtcggtgtt
    23461 tgtacacgac caagagaaaa actgccatca atcccttttc gttgaagtag caagctatat
    23521 aatggacttc cattcattcc aatcagccaa tcagcgttct gccttgcttg cgcttcttta
    23581 tattttggaa aatagttctt tccaggttgg agattcttaa aaccttcata gattgcttct
    23641 ttttcaagag aattgatcca taaacgtaaa tagttctttt ccttagaaaa tgcgtttgct
    23701 tgtcgaataa tcgaccacgc gatggcttca ccttctcgat caccatctgt tgcaataatg
    23761 atggtatcgg ctgactgcaa ttctcgtttt acgatcgaaa attgtgcacg tttgtccatt
    23821 ggaacttcgt aacggaaggt ttctgggaaa ataggcaaat tttctaaaga ccattttgcc
    23881 caacgctctt cataagctcc tggagcaacc atatctacca aatgtccaaa accataagtg
    23941 atggttactt caccagcaaa caatggatcg ctgatttcat aatacccttc atgccgcttt
    24001 tgctgttgga aggtttcggc ataggctttt gcttgtgaag gtttttcagc tagtatcacc
    24061 gtcttcatct tctgacctcc gttacttaat tttttgcgga cttctctatt atggaacata
    24121 ttttcgcatc tggcaataaa aaaaagatac cgtatagtcg aaatagacac aaaaacatca
    24181 agcaaacaaa cgatgatccg tttgctgctt gatgtttcca gaaaatattc atctttagct
    24241 gtcactcttg gttaatctgc cagttgatgc caaatcgatc agttaccatc ccatacaact
    24301 gagcccacgg aacagcatcc agtggcatct caatcttacc accctcagct aagctttcaa
    24361 ataggcgctt cgtttcttct tcatttcttg tctggatgac taaagaaaca ttatttccca
    24421 caactaattc aggaccaatt ccttctggcg tatctgcaaa cataactaat gtatcataaa
    24481 tgttcagccg tgcattcgtg attagatttt ttgcttcttc tggcaagttc atttgttcta
    24541 ttccaggcat ttcaccgtat gtggtgatac ctgatgcttc agtctgaaaa actttttcat
    24601 aaaaagcgat ggcttcacgc gactcatttt tgaagttcag atagatatcc aatgtaaaca
    24661 gctcctctct tggttaaatc taccataaga aagcgttttt tacgagtgaa tcgtttgatt
    24721 gattcatgac aaaagccttg cgcagcttct tcttgatctt ttttatgatc atttattcaa
    24781 aaaatctgta atagcagatt gattcatttg attatcaatt tcaagaatac tgccagcata
    24841 atcactttgc ccataattcc acgaaccgtc tactggcaca cttattcgat caatgccacc
    24901 agctcctcgt gcaatcgaaa agaggttttt cgtcaagaac caagcagtga catctgtgga
    24961 tgtatatcct aaagctttac cagctgcata aggtgcacgg atcagattca atggattctt
    25021 caattggctg aaaatagcgt tcattacttg ctgttggcgt ctgacacggc caaagtctcc
    25081 ttgttcatcc attcggaaac gtgcgtactg caaaagaaca tgtccatcca tcttctggac
    25141 ccctttttta atatctacac catccagatt cagatctttt tctgcatcga tcttcactcc
    25201 gttcatgaat aaggcatcga tcactttttc aaacgatttg aaatcgactt tcgcatagta
    25261 ttgacaatcg attccaaaat tttctactaa agtttgacgc actagatcgg ctcctccgta
    25321 tgcataagct gaattgatct tattttgtcc tacccctggg atcgtaacga agctgtcacg
    25381 cataaaagaa atcagtttcg gcttatggga aggaccatcc aactgtaaga ccatgatcgt
    25441 atcggctcgc ccggcatctt ctccgcgtgt atcacttcca aggatcaaaa tattatgcgc
    25501 accattcgtc gctttttgcc catgaaaggt ttctgaagtc gctgcctcta agcttgtgtc
    25561 atgttcagct acatatttac caataaaaaa tgagatacct gaataaagga tcaaaacaag
    25621 caaaatcgat agaatgattt ttttccacga tcgctttctt cgaggtttta acggtttgtt
    25681 cttcttttgc cgattgaaaa attttttatt tttttgttgt tcttctgctc ttggtgcatc
    25741 tctgtatatc tgttgctcgt attggttcaa atgttcaaat gaatgctgct ctctttcata
    25801 aatacgggta gcatcttcct cattttccat acgcgttcgt tttcgttctc ttctagcaaa
    25861 aatactcccc gtatcccgct tcagaggttt cgccttctta tggatgtcct tatagcgatc
    25921 catccgactc attgtggttc ctcctcgttt cttactgata aataaaggat acgataacca
    25981 atcgacaaaa gcctatcatt ttttgataat ttaataaaaa ataacgaaat aaatgcttcg
    26041 atacataaag gattactttt cttgagaccg tttatgcgag aataaaagat ctttatctat
    26101 tatccttttt taatcaaaca gcttttcaat cacatggatc ggcatctttt cctttgtcca
    26161 taattcaaac gcgattgctc cttggtagat caacatgcca agtccattgt atccttttgc
    26221 tcctactttt tttgcttctt ttaataaacg tgtttctcta ggtgcataaa tcaaatcaaa
    26281 aaccgctaga ttctcatgca tcatgcttat atctaagagc ggtgactgat tgattttctt
    26341 tcccatcccg atatctgttg cattgactag aagacagctt tctttcaagt cttttcttaa
    26401 ttgatgcgta tcggcaaagt cacgaacttt tatcttacaa gaagttcgat agctgagttg
    26461 ttcgaatgtt tcttttactt gagcgaatgt ttggttgttt cgtttataaa ctacgatttc
    26521 ctcagcgccg tctaacgcag cttgaacagt gactgccttt gctgcacctc cagctcccag
    26581 tagagtcatt tttttctttt tgatcgaaat cccagcttct tgcacggatt gcatgaagcc
    26641 cgcaccatcg gtattatagc caatcaaacg acccttttcg tgcacgatcg tattcactgc
    26701 tccgatcaat tgcgcttcct ggcttacctc atccagataa ggaataaccg ctgttttatt
    26761 cggcatcgaa agatttacac ctaacatatc aaaagttcga atactttcga ctgcttgctt
    26821 caaattcatt ggatttactt ggaaagctaa atacaccgca tcgatgttgt ttgcttcaaa
    26881 agcagtattg tgcatcaatg gggataaact gtgagaagcc ggatcagcaa agagcccagc
    26941 taatttggtt tgtccagaaa tcatcttatt cctcctttgt tcttacttca ataaaaaaag
    27001 ccacttagcg tttgatacga aaaagtacga aacgtctagg tggcaaagta gcaaattctt
    27061 ttctcatcgt ttacttgtca gagcaaagaa tattctattt aattgttaaa atcattgact
    27121 acgtgatcct acaaaacacc caaatat
//
LOCUS       DACCQI010000028        27083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.028, whole genome shotgun sequence.
ACCESSION   DACCQI010000028 DACCQI010000000
VERSION     DACCQI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.028"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IVO76_000927"
     CDS             7..624
                     /locus_tag="IVO76_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428908.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="IVO76_000928"
     CDS             1644..2813
                     /locus_tag="IVO76_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7428909.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="IVO76_000929"
     CDS             3286..3840
                     /locus_tag="IVO76_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7428910.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="IVO76_000930"
     CDS             complement(4013..4486)
                     /locus_tag="IVO76_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7428911.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="IVO76_000931"
     CDS             4498..5034
                     /locus_tag="IVO76_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7428912.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="IVO76_000932"
     CDS             5074..5445
                     /locus_tag="IVO76_000932"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7428913.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="IVO76_000933"
     CDS             5729..7000
                     /locus_tag="IVO76_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP7428914.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="IVO76_000934"
     CDS             7245..8270
                     /locus_tag="IVO76_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7428915.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="IVO76_000935"
     CDS             8564..9673
                     /locus_tag="IVO76_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7428916.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="IVO76_000936"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="IVO76_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="IVO76_000937"
     CDS             10668..11492
                     /locus_tag="IVO76_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7428917.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="IVO76_000938"
     CDS             11524..12834
                     /locus_tag="IVO76_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP7428918.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="IVO76_000939"
     CDS             12982..14241
                     /locus_tag="IVO76_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7428919.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="IVO76_000940"
     CDS             14251..15123
                     /locus_tag="IVO76_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7428920.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="IVO76_000941"
     CDS             15135..15965
                     /locus_tag="IVO76_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7428921.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="IVO76_000942"
     CDS             16005..18188
                     /locus_tag="IVO76_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7428922.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="IVO76_000943"
     CDS             18249..19469
                     /locus_tag="IVO76_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP7428923.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="IVO76_000944"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="IVO76_000944"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP7428924.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="IVO76_000945"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="IVO76_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="IVO76_000946"
     CDS             22431..22640
                     /locus_tag="IVO76_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7428925.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="IVO76_000947"
     CDS             22633..22782
                     /locus_tag="IVO76_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP7428926.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22817..23601
                     /locus_tag="IVO76_000948"
                     /pseudo
     CDS             22817..23601
                     /locus_tag="IVO76_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IVO76_000949"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IVO76_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7428927.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="IVO76_000950"
     CDS             25304..25537
                     /locus_tag="IVO76_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP7428928.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="IVO76_000951"
     CDS             25534..25941
                     /locus_tag="IVO76_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7428929.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26392..26901)
                     /locus_tag="IVO76_000952"
     CDS             complement(26392..26901)
                     /locus_tag="IVO76_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428930.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT         9318 a         4024 c         5037 g         8704 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatact tggatatgct ggaatgttta agagaataat tacatttcta
    26101 gaaaatacaa taaaaattac aggataatta gccatatcct acctcctcta gcagttgctc
    26161 aaaagttagc actaagtaac ttcttttttg ttgctggtaa caatttatca atactaaaat
    26221 agcgtttaag tatctaataa atactaattt taaagctctt aattattatg aaacacgata
    26281 taacaatatt gctatatcgt gctattgtaa tattatacat ttgaagacag cgtattcttg
    26341 ttctaatatc aggaattttt tataaatatc agtcatatta agactcaaaa tttattggta
    26401 tgttcctctg tatagagttt gaccgtctac cattatatag caattattat cattgttgtc
    26461 ttgcaccaat ataccgttaa gggtagcact aggagtagta acaatcactg gaaactcatg
    26521 agaaagctcg gtcatttttc ctgttctgct agaatcgtgt tctattgtca agccatttac
    26581 tataaacgtg aatactttac ttcctattgt tatagtagct ttttgagaaa cagtttctgt
    26641 agctggagct gtgcttcttt ccgaatttgt tagggtttca ctggattcat tgctataaac
    26701 aattgtagag gattctacgg cttcttgagt gtccacgttg gtgctgctag cttcatctag
    26761 cttccttttg tattgttcgt tactgattgc taagctttcc cgttggtcat caagcttatt
    26821 catttctgta atggtataaa ataatcctcc tgctagtgct atattaacaa ctaaactaac
    26881 cgcaaataat acctttttca ttcttccttc tcttctttct ctttttgtaa ttccttactt
    26941 ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaacat
    27001 accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca
    27061 acaaatcgtc aatctttact tag
//
LOCUS       DACCQI010000029        27052 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCQI010000029 DACCQI010000000
VERSION     DACCQI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.029"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..507
                     /locus_tag="IVO76_000953"
     CDS             <1..507
                     /locus_tag="IVO76_000953"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="catalyzes the addition and repair of the
                     3'-terminal CCA sequence in tRNA; these proteins belong to
                     the CCA-adding enzyme subfamily 2 which does not have
                     phosphohydrolase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7428931.1"
                     /translation="EGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLKSW
                     KCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDAVG
                     ESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQVLD
                     FLSKEKLI"
     gene            661..1116
                     /locus_tag="IVO76_000954"
     CDS             661..1116
                     /locus_tag="IVO76_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428932.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            1478..1753
                     /locus_tag="IVO76_000955"
     CDS             1478..1753
                     /locus_tag="IVO76_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428933.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            1766..3844
                     /locus_tag="IVO76_000956"
     CDS             1766..3844
                     /locus_tag="IVO76_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7428934.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            4004..5890
                     /locus_tag="IVO76_000957"
     CDS             4004..5890
                     /locus_tag="IVO76_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7428935.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            5902..6849
                     /locus_tag="IVO76_000958"
     CDS             5902..6849
                     /locus_tag="IVO76_000958"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7428936.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            6869..7396
                     /locus_tag="IVO76_000959"
     CDS             6869..7396
                     /locus_tag="IVO76_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7428937.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(7459..8112)
                     /locus_tag="IVO76_000960"
     CDS             complement(7459..8112)
                     /locus_tag="IVO76_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7428938.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            8244..9086
                     /locus_tag="IVO76_000961"
     CDS             8244..9086
                     /locus_tag="IVO76_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7428939.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            9244..10140
                     /locus_tag="IVO76_000962"
     CDS             9244..10140
                     /locus_tag="IVO76_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7428940.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            10143..10856
                     /locus_tag="IVO76_000963"
     CDS             10143..10856
                     /locus_tag="IVO76_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7428941.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            10876..11394
                     /gene="msrA"
                     /locus_tag="IVO76_000964"
     CDS             10876..11394
                     /gene="msrA"
                     /locus_tag="IVO76_000964"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7428942.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            11391..11618
                     /locus_tag="IVO76_000965"
     CDS             11391..11618
                     /locus_tag="IVO76_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7428943.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            11770..12321
                     /gene="lepB"
                     /locus_tag="IVO76_000966"
     CDS             11770..12321
                     /gene="lepB"
                     /locus_tag="IVO76_000966"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7428944.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(12404..12946)
                     /locus_tag="IVO76_000967"
     CDS             complement(12404..12946)
                     /locus_tag="IVO76_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7428945.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(13183..13581)
                     /locus_tag="IVO76_000968"
     CDS             complement(13183..13581)
                     /locus_tag="IVO76_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7428946.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            13841..14701
                     /gene="ylqF"
                     /locus_tag="IVO76_000969"
     CDS             13841..14701
                     /gene="ylqF"
                     /locus_tag="IVO76_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7428947.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            14694..15461
                     /locus_tag="IVO76_000970"
     CDS             14694..15461
                     /locus_tag="IVO76_000970"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7428948.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPCYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            15517..16374
                     /gene="dprA"
                     /locus_tag="IVO76_000971"
     CDS             15517..16374
                     /gene="dprA"
                     /locus_tag="IVO76_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7428949.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            16496..18574
                     /gene="topA"
                     /locus_tag="IVO76_000972"
     CDS             16496..18574
                     /gene="topA"
                     /locus_tag="IVO76_000972"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7428950.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            18543..19919
                     /gene="trmFO"
                     /locus_tag="IVO76_000973"
     CDS             18543..19919
                     /gene="trmFO"
                     /locus_tag="IVO76_000973"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7428951.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            20117..21022
                     /gene="xerC"
                     /locus_tag="IVO76_000974"
     CDS             20117..21022
                     /gene="xerC"
                     /locus_tag="IVO76_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7428952.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYCSFHPRA"
     gene            21059..21607
                     /gene="hslV"
                     /locus_tag="IVO76_000975"
     CDS             21059..21607
                     /gene="hslV"
                     /locus_tag="IVO76_000975"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7428953.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            21621..23021
                     /gene="hslU"
                     /locus_tag="IVO76_000976"
     CDS             21621..23021
                     /gene="hslU"
                     /locus_tag="IVO76_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7428954.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            23039..23830
                     /gene="codY"
                     /locus_tag="IVO76_000977"
     CDS             23039..23830
                     /gene="codY"
                     /locus_tag="IVO76_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7428955.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            23912..24787
                     /locus_tag="IVO76_000978"
     CDS             23912..24787
                     /locus_tag="IVO76_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7428956.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(24919..25557)
                     /gene="plsY"
                     /locus_tag="IVO76_000979"
     CDS             complement(24919..25557)
                     /gene="plsY"
                     /locus_tag="IVO76_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7428957.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            25684..26118
                     /locus_tag="IVO76_000980"
     CDS             25684..26118
                     /locus_tag="IVO76_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7428958.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            26284..>27052
                     /locus_tag="IVO76_000981"
     CDS             26284..>27052
                     /locus_tag="IVO76_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7428959.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEV"
BASE COUNT         9414 a         4637 c         5701 g         7300 t
ORIGIN      
        1 gaaggcgaag cacttttacg ttttgctgaa ttaccatccg tcccattgaa agaagagagc
       61 caagcatggg cattattgat cagccaactg gatctaaaag aaaatgagat tcgttccttt
      121 ttgaaatcgt ggaagtgttc taaccaaatg attaaagacg ttcaagcact tgtccaagga
      181 ctacgacaac gagaaactgg agctttggaa ccattcgaat tgtacttctt aggcaaaaaa
      241 catgcactgc aagtagaaga attgatgatc tattttgacg aaacacctca gatggatgcc
      301 gtaggagaat cctatgatcg tttacctatc aagtcattaa gcgaattagc agtagatggt
      361 aaaatcttgc tagaagaaac tggaaaaaaa cctggtaagt gggttagtga agctttgcaa
      421 ttagctgaaa aagccgtcgt agaacaaaga ataaaaaatg ataaaaaaca ggttttggat
      481 tttttatcga aagaaaaatt gatttaaaaa acactagact gtatctgctc agctttgttg
      541 gctacttaaa gtgggcagat acgtcattag aagaaaaaac ataagatgtg accacttaca
      601 taaatttgtg ctaaactgag gtagtacaag aataaattac atcataaagg agtcttttta
      661 atgagtaaag aaatgacagc gctaaaattt tattttagaa atggagaaac ctggacgatc
      721 aaccgccgtc atatcggcga tctttggatc aaacaaatca caacaagttt tggacgaatc
      781 aatggcagtg aatttgttga aatccatccg tgtgctggat ttaaaatcga aatcttccat
      841 gaaggggacg cagttgcgac acatgatatc aacctaggcg gactggaaat gggcatgttt
      901 aatcgcgcat tgaaatacga agatatcgag cgaatggaaa tcctttaccg aaatggcaca
      961 cctgatcttg tgtacttccc atatctagat aaaggaacag aaggattaga caatcaatat
     1021 caatcaacaa aaatcagcga aaaaacaggt aacttgtata tcgtgatcaa tccagatcaa
     1081 cgtgtagaag atgtatacgg ggaatttttc gaataaatcg atttttacgc aaaaatgagg
     1141 ctgtgaaaga gatgtgtctt attactactg ataggctgtc tcttccacac cgtttattca
     1201 gaataaggga ctgtgacaag atttttgtcg cagccccatt tttttgttgt gataaaagaa
     1261 caagaaaggc aggaatggga aagtaaacat ccttttgtca gaaactttta tctttcttaa
     1321 gttttacttg atatctgata ctatttggaa tagaatgagg cgttgaaaaa tttatggaaa
     1381 tttctaacta gcaataaacg atgaaacaat gaaatatttt tcatccaaaa agaactttgc
     1441 tataatgatg gaagtttgtg tataggagat ctggaaaatg agagagagtt ttgaacaaca
     1501 aaaaaaactg ttgcatgatc gctatggcgc cctttcgatg gatgaccgcc gtcaaatact
     1561 ttgtaagttg cgtaaaagaa atatattgat gtatcgtcag ttggaacgtc tgaaacatga
     1621 tctgcttcgt ttggagtcaa aacgtgtaca gtgtgaatta gaggggaacc aaacacaggt
     1681 tgaagtagtc gaaaccaaaa ttttaaagaa aaaagaacaa tttttaaaaa tgcttacaca
     1741 aaataaaaaa taaaggggat ggacgatgga atttgctgag ttagttattg tatttgcagt
     1801 aacgatcacc ttatcgaaca tagctagccg cattttgcca atgattccag ctccactgat
     1861 ccaaattttc cttggagtgg tattaggtct gacagagtgg ggacaatcaa tcgattttga
     1921 acctgaattg ttcttagtta tgattattgc accattgtta tttagagaag gagaaaaagc
     1981 agatatttct tcgattctca aaaatttcgg gacaattttg ttcttggcat ttggaggagt
     2041 cattttgacc cttgtcgggg taggtgcgac gttatcattc ctgttgccaa gtgtcccact
     2101 tgctgcctgc tttgcttttg gagcagcgtt aggtccgaca gatgcggtag cagtcagttc
     2161 tctttctgga cgagtgaata tccctaaaaa ggcgatgcat attttggaag gagaaggtct
     2221 tttgaatgat gcatctggtg ttactgcctt ccagtttgct cttggtgcgc tgatcaccgg
     2281 atctttttca gcagtaaacg cggggatgag tctagtagtt tctagtattg ggggagcttt
     2341 gatcggcttt ttgcttgtct ggttcaaaca aaaaataatt catttgatcg aaaaagcatc
     2401 agcacaagat gtcacagggt atctgctgat cgaacttttg cttccttttc tagcctatgt
     2461 attggcagag gcatttgatg tatctggaat cattgctgca gtggcagcag gtattctcca
     2521 agcttctggt tttaggaaaa tctctgtgtt tgatgcagaa ttgtcgagct tgtcccacag
     2581 tacatggaca actattgctt ttacactgaa tgcattagta tttatatttt taggaatcga
     2641 attgacgcaa gtgttctcgc cagtatgggg agacggttta tatccaaacg gtcttttatt
     2701 agcgattatc gtattaatct ctgtcatgct ttttgtgatc agattcattt ctatcagtct
     2761 tttttatgta ttcaaagatg gcagtaaaaa gttcaaaaaa cagttgaacg agatcttgat
     2821 cttaacattt ggcggtgtaa agggaacagt cagtttggct acgattttta ttttgccatt
     2881 ttcaatcaat aatatgatgt tttaccagcg gtctttgctt ttgtttctga cggcgggtgt
     2941 catcttagtg actttggtca tagggatcat tgtcttgcct atgctgacgg aaacagagga
     3001 agcgaaaagc acggatttga atgctttgat gattttagaa gaggtcgttg aagttttgcg
     3061 aaaagaaata aaggaaattg atcgtaatac aaaagaattt ttagccactg aagcagtgat
     3121 agaaaattat caagaacgga tacgtgatct gtacttggaa gatttaaccg atgatgaaaa
     3181 gcaagaagta caagaaatac aagcactgat tttgtcgatc gaacgggatg gattggatga
     3241 aagttatcgt tcgggcaagc tttcttctaa tggttatcgt ttttactccc gttttctttc
     3301 tcgattcgaa cattctatca caagccagat tttgtctttt atcggctttt ggttcattgt
     3361 tgtccgtagg ttaatgcgaa tcgttctcca tccgaaaatg ttttggcaaa gaagatatgc
     3421 tgatcgtcag acatttatta gtgatgaaga tatccaagaa atcagagaga catatcaaaa
     3481 aaatactcag ttgattattg aaagtttaga taacttgact gatatttatg atgaaacgat
     3541 ggttcaattt tttatccagc aacgcaaact tgaaggaata aaaatgaact cggggaattt
     3601 gatttcttcg tggatgatcc agcaagatag tctgtttacg aaaaaaatgc taagaggtta
     3661 ttatcttgaa agaaaaatca ttgatgaata cgaagttgct gaaaaaatca cgacgttttc
     3721 tgcgaacagc tatcgcagga atatcaattt gttagaatct tatacgatga acaaaccatc
     3781 tgataacttt tctttccgat ttgcgtttcg agcaaagtca aaaaaaacga gtccaataaa
     3841 ataaaaaggt aaaaattttc aagttttata aaaaacaaaa catatcagaa caggatcgaa
     3901 tcggtcctgt tctttttttg agaacacaaa aagaacaaat accaaaaaaa ttttcctgaa
     3961 gttcactatt tttatgatac aatatccaag ataaggaggg aacatgttga aagaattaaa
     4021 agtaactgac ctcaaaaaaa cttatggtga aaaagatttg tttgaccaga tttctttttt
     4081 ggtccacgaa aaagaccgta tcgggttgat tgggacgaac gggacgggta aaacaagtct
     4141 cttaaatatt attgccggta tcgatagcag cgacggtgat cggcaaacag tgttttatcc
     4201 aactgattac cgtattggtt atctttctca agcagctgaa ttttccgatg aattaactgt
     4261 tctccaagca gtctttcaag gaaacagccc cttgatccaa actgttcgag catatgagga
     4321 agcattgatc gaactagggg aaaacggaga agaccctgat gtgcaaaaac gttatgctaa
     4381 agcagaagaa caaatgaata aagaagatgc ttggactaca gataccaacg caaagattat
     4441 tttgcaaaaa ttaggaatca gtgaattaga taaaaaaatc agtactttat caggtggtca
     4501 gaaaaaacga gttagtctgg cgcaagtgtt gattgatgaa cccgatttac tattactaga
     4561 cgaaccaaca aaccatttag attatgaagc gatcgagtgg ttggaaaact atttgaaaca
     4621 ataccgtggc gcactattga tggttaccca cgatcgttat tttctagata gagtagcaaa
     4681 ccgaatcttc gaactttcat ttggaaaatt atacgagtat aaaggaaatt atgaagctta
     4741 cgtcttagaa aaagcagaac gtgatcgtgt agaagtagaa caagaagaaa agcgcaagcg
     4801 gctttataaa caggaattag cttggatgag ggctggcgta caggcacgtg gcaccaagca
     4861 gcaagcacgg atcaaccgtt tcgaagatct aaaggagaac ttgtaccaag taaaccaaga
     4921 agatgattta gagctgaacc tagcgacaca acgtttaggg aaaaaagtac tggaaatcaa
     4981 agatggcagt taccgtataa atgaccaaac actgctagaa catttagatt tattgattca
     5041 atctcgggaa agactaggta ttactggtaa aaatggtgcg ggaaaatcga cattgcttaa
     5101 tattctagct ggcagaattc ccctggatag cggtacaatg tctatcggtg aaacggttca
     5161 tcttgcgtat tatacacaag aaaatgaaga aatggcacca gataaacgga tgattgctta
     5221 cttacaagaa gctgctgaag aagcaaaaac agcagatggc agtcaaatcg gagtagcaga
     5281 attacttgaa cgatttttat ttccaagatt tatgcatggg acactgatag gcaaactttc
     5341 aggcggagaa aaacgccggt tatttttgtt gaaactgctg attcagcaac ctaatgtttt
     5401 attattggat gaaccgacca atgatttgga catagcaaca ttgacgattt tggaagatta
     5461 ttttagaagt tttccaggtg ctgtgatcac tgtttcccat gaccgttatt tcttggacaa
     5521 ggtagcagat aagttattag tattccaagg aaacggaaaa caagaacttt attacggaaa
     5581 catgtccagt tatttactga agcaaaaaga gacccagcag cctgctgaaa aagcgaaacc
     5641 aaaaatacag agcaaggaac ctgctgaaaa gaagaaatta tcttacatgg aacaaaaaga
     5701 atgggaaacg attgaagatg agattgcaga acttgaagaa aaaatctcat tgctccaaga
     5761 agagatgaat catcaaggag ataatttcac tcgattacaa gaattgcaaa acgacgtatc
     5821 tgaaacagaa gcacaacttg aagaaaaaat ggcacggtgg gaatacttga gtgaatgggt
     5881 agaagattag gaggaaaaag gatggaacaa gcgtatcttg acttaggaag aaaattgttg
     5941 gaagaagggc atcgtaaaga agacagaaca ggcacaggca cattaagctt attcggatac
     6001 caaatgcggt ttgatctgca aaaagggttt cctttgttaa caacgaaacg agtacctttt
     6061 ggattgatca aaagcgaatt gctttggttt ttaaaaggag atacgaatat ccgctatcta
     6121 ttacaaaata aaaaccatat ttgggatgaa tgggcatttg aacgctacat caaaagtcca
     6181 gactataatg gaccagatat gactgatttt ggccatagat gtttgacaga tcctgaattc
     6241 aatgaagaat ataaaaagca gagcaagctt ttttgcgaga agatcctaac cgatgattcc
     6301 tttgctgaaa catacggcga tcttggacac atctacggct atcaatggcg tcactgggaa
     6361 acaaaagacg gtggctttat cgatcaaatc aaacaagtga tcgaagcaat caaaaagact
     6421 cctgattcta gaagattgat cgtttctgca tggaatccgg aagatgttcc tagcatggct
     6481 ttgcctcctt gtcatacgat gtttcaattt tatgttcaag agggacggct aagctgccaa
     6541 ttgtatcaac gcagtgggga tgtgtttcta ggcgttccgt tcaacattgc aagttatgcg
     6601 ttgctgactc acctaattgc acatgaaaca ggtttagcgg ttggcgaatt tgttcacaca
     6661 ttaggagatg ctcatcttta tatcaatcat atcgaacaaa tgaaagaaca attgagccga
     6721 gaaatacgcg catttccgac tttagttttg aatcaggaaa agcagtcagt atttgatttt
     6781 gagatggaag atatccaatt agaaggatat gacccacatc cggcgatcaa agctcctatc
     6841 gctgtttaga atcagaaagg atgagaaaat gttagctgca atttgggcac aggatgaaaa
     6901 cggattgatt ggaaaagaag accgtcttcc ttggcgtttg ccaaatgatc tgaaattctt
     6961 caaacaaatg acagaagcaa atacactagt gatgggacga aaaacattcg aagggatggg
     7021 tagccgtccg ttgccgaatc gtcagacgat tgttttgacg agagattctt cctataaagc
     7081 agaaggcgtg catgtcatgc atagtgtgga agacgtgctt gctctggaaa aagaaacaga
     7141 cggtatcttt ttcatcgctg gagggtcagc tgtttatcaa gaatttctgc cattttgtac
     7201 gatattatac cgtacagtca tccatcatgt attcgatgga gatgcttatt ttccacctgt
     7261 tgactggtct gattggtcac tgatcaatac tagtcaagga gaaatggatg aaaaaaatcg
     7321 ttatccgcat caatttgaaa cgtatcagcg taatgagaat cctgtaaaaa gcgggacagg
     7381 cgaattgacg atataaaaaa gctatggata gactagctgt tatttagagc tgtcccatcc
     7441 atagctttat tttttttatt aagtaaagaa taaaacagag aagtacataa aacctgcacc
     7501 taacataaca aacaaatgcc aaacaacatg catatagcgg atagaattca agctataaaa
     7561 gaatgcgcct atcgtgtaag aaagcccgcc tgctaccatc aaaataacac cagtcattcc
     7621 aagagagtga taaagaggaa cagcagccac gacacagagc caccccataa aaatatagat
     7681 gatcgtagat acttttgata ctctctcccg tttatgcaac gtcaacgctt tatatataat
     7741 gccaagaatc gcaaacaacc agattaatac aaataaagtc caacctagcc atcctcggat
     7801 actcagcaaa caaaaaggag tataacttcc tgcaatcaaa agaaaaatcg aactatgatc
     7861 gaatacttgg aacacctttt ttgcttttgt aaaaatcaat gaatgaaaca aagtagacgt
     7921 taaaaataaa agaatcatca tcgatccata gatagcataa gagacaatat ggagggagga
     7981 atgcaagtgg gcacctttta ctaaaagaac cactagtccc gcgatactca atccagtccc
     8041 aattccatgg gtgactgcat taaacacttc atttaatatt tcatacgttc gattaaattt
     8101 cgttttttcc atgaaaacgg ctcctttact aggtgtaatt aatgaaaaat cattatttgt
     8161 ctgttatact aaaaatagaa attgtcttac atgtagttta acatgaataa ggcatcttgt
     8221 taaagaaaga gagagagaaa ataatgacaa atgtaaaaat cgtaacagat tcttcatgta
     8281 cgatggaaaa aaatgtacgc gatgctttag gcattcgtgt catgccgcta tccatcatga
     8341 tcgacggaac agtatatccc gatgatgacc aactagacgg cgaaagattt atggaaatga
     8401 tggcgaaagc aaaaaatctg cccaaaacaa gccaaccacc aattggagaa ttcatctcga
     8461 tgtatgatga gctaggagaa gacggcagtg aagtgatttc gatccatatg acaaaaggct
     8521 tgagtggaac agtagaagca gcaagacaag ccagtaatct aacaaaaacg aaagtaacgg
     8581 ttatcgacag tgataccact gaccaaggat tatcttttca ggtaattcga gcagctgaat
     8641 tagcaaaaga aggtaaaaca gtagagaaaa tccttgaaga agtcaaaaaa gtacaagaca
     8701 atacaaaatt atacatcgga atatccacac tagataactt ggtaaaaggt ggaagaatca
     8761 gccgaacaac tggtttgctg tctagtgttt tgaacatacg cgtggtgatg aatttcgccc
     8821 attctgaatt aatccctgta actaaggggc gaggaaagaa aacgttcgat aaatggttta
     8881 ataatttgaa aaatgaactg aaagaattgc caaatgtgcg acaaatcggt atttcacatg
     8941 cggatgcaaa agaattggcg caagaattca aagagggttt gcaagaatta ttcccggaca
     9001 tgcatatccc tgtactgcat acgaatccgg ttatcgctac gcatactggt aaagacgcgt
     9061 ttgcaatcat gtattatgta gattagtcga aaagaggata agccctcagc ctgaaaggct
     9121 tgcagcttat tctctttttc tttataaaat taggaaaata attgttttct tttttcataa
     9181 agactacctt tttgataaaa tttttgataa cattagaacc aatcgaatac aagaggtgaa
     9241 aaaatgaaat taacgaaacg acatttgatc ctcgttttgt tcccattatt aattgcgatc
     9301 atcatctatg caggtttagc tgcttctata ccgaaagctg gcccactaat gaaaccagta
     9361 actactgtga gagaagatcc aaataaaaaa gaaaaacttc actacatagc tatcggagat
     9421 tcgctgactg aaggagtagg agatcaaacc aagcaaggag gattcgttcc ccttgtcgca
     9481 aatgatctaa aagaccgtta taatttgacc gcggttgaaa cagaaaacta tggtgtaaat
     9541 ggtgaacgaa gtgaccagat cttaaagcga gtaaaaacaa aagaagaaat tcgaaacaat
     9601 atcaaaacag ctgattttat cacactgact gttggcggta atgatttgat gaaagttatc
     9661 caaaataatt tattcgggtt aacggtcaat tcctttaaaa agccactgaa aaactatcag
     9721 gaaaatgtca cagaactatt ggaagaattt cgatccctca ataaaaccgc acctatttac
     9781 attttaggca tctacaatcc attttatttg aactttcctg agatcactga catgcagaca
     9841 attgttaaca attggaacaa aggaacagaa acggtgacaa aaaaaatgcc gaactgctac
     9901 tttatcccaa tcaatgattt gctttatcaa ggattgaatc aggaagtagg tgtcacagaa
     9961 gaaggaacag atcaaacaac tagcacgaca gaaaatctaa atggcatcaa aaataatgtg
    10021 ctctatgaag aagatcattt ccatccaaat aatttaggtt atcaattgat ggcaaatgca
    10081 gtgagagatc agctgatcga gacacaaaac caatggttag agaaggagga tgatcaataa
    10141 acatgaaaac aagatctgac gtatcttcaa acaaaacaaa gaaaacaaat tactggaaat
    10201 tcgccttcct tatccttctt ggggtagtac ttggttcgac cgtattttta ggcagccgta
    10261 tttttgctaa tcgggaacca gaactcccag aaataccagc tcttacggaa agacaaggag
    10321 atcctgtctt aacgatcaat tcaaataaag aaaaagtcaa tcaaatcatc agctttttcc
    10381 ttagtgagta tcaaaaagat agtgatattg aatataaatt ttatttggaa aatgaagcac
    10441 tactcaatgg tactttcgaa gtactaggtt ttcctatcga tttctattta tattttgatc
    10501 cgtatgtgat ggaaaacgga aatgtccaac taaaagctag aagtctttct atcggaacat
    10561 tgagtttgcc aatcaaagat gtgatgaata tgatcaagag aaactataaa ttaccagatt
    10621 ggatcgaagt caatacagat gatctgacta tcatgcttag gttggatcaa ttccgaatgc
    10681 aaaacgggat gtatattaaa gcagataaaa tcgacttagt aaatgatgac attcggttta
    10741 gtctatattt gccaaaagat acctctgatg cttcaaacaa ctcaaatacc aacaaaacta
    10801 gcaccagcag tactgaaagc aagacaaaag aaaataaaaa atcatcggat gagtagtatt
    10861 agagggggaa tcataatgga acgagcaata tttgcaggag gctgcttctg gtgtatgatc
    10921 cagcctttcg acacgcttcc tggtatccat acagtgatgt caggatatac tggagggcat
    10981 gtaccagatc cgacttatga acaagtaaaa agccaaacga ctggacatac agaagcagtg
    11041 gagattatct atgatccaac attgatttca tatgaacaat tagtagaact ttactggcag
    11101 caaactgatc caactgacgc tttcggacaa ttcgaggatc gcggtgacaa ttatcgcccg
    11161 gtgatctttt atttgacaga agaacagcgg atcattgctg aaaaaagtaa gaaacagtta
    11221 gctgaaagtg gacgtttcga acagccaatc gttacaaaaa tcgaaccagc ccaaccattt
    11281 tatctagcag aagaagacca tcaagagtat tataaaaagc atcctgaaaa ttttaaaaga
    11341 aaccatgcac gccgggctgc ctttatcgaa gaaaattgga ggaacgaagg gtgaatcgaa
    11401 gcttttttca ttatttaatg acactgcgag gcggtggcac acatgatgct ttatctgttt
    11461 tcgctacaga agccggtaaa gacatccaat ttcctaagca cagctctagc tacgaagaaa
    11521 tttctaatta tctggaactg aatgtcgact atttgccgtc catggatatc tttgacgaag
    11581 catgggaaaa atatttagaa aataatcatg acatataaga gatagttttg tactttatga
    11641 tcaaaaaagt tttttgtcca atagcctaat tgaaagaatt atttttattt cagctatcta
    11701 gtcgcatttt tttgatattt ttcttataat aaagaaagaa tgaaaaaaag agggatggat
    11761 tgatagagat tgacaaaaaa acaacgctat attaatcgct tctggcttgt attcaaatac
    11821 ttacttgttt ctgtattcct tgcgttcatg ttgcggggat ttttattcat cccggtacca
    11881 gtggaaggca attcaatgga aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa
    11941 ttttcggaga tcagacgatt tgatattgtt gtttttcaat tagcagatgg cacgatttat
    12001 atcaaacgag taatcggtct tccaggagaa aatgtcagct accaaaatga tcaattgaag
    12061 atcaatggga aagtagttaa ggaaccttat ctaactaaaa acctgaaatc agatcatgca
    12121 aatgcatcct atacgacgga ctttacacta caagagctaa cgggacaatc caagcttccg
    12181 gaagataatt attttgtact tggagataat cgaagagttt caaaagatag tcgttcgttt
    12241 ggcacgatca ataaaactga tattctaggt aaagcaagat ttgtctatta tccacttgat
    12301 gaaataaaat ggatccagtg agaaacaata aagcgtgcag tctgttgaga ctgcacgctt
    12361 tattgtttaa tccaaaatca gtttactacc ttagtacgta aaatcaaaca gctcgtttaa
    12421 acacttttct agcctctaaa gttttgataa atgaataaac aaccattatt tgaataggga
    12481 tttggatcat attcctgatg aatctaccta gaaaaattac tttgaaatct gcaccaaaca
    12541 aaacagacat cagccaagaa ttgataaaaa ggctgactgg gatcataatc aataattggg
    12601 cagcgatgac ggaaggcagg tttatctttt tatgatataa aaataagcca ttaataaatc
    12661 caccaaggaa agccgtgata agaaaagcag cttgaaacgt tcctgaacca aatatcgtga
    12721 aatttaaagt gttcgacaac atttcaagca aaccagccca gatcggtcca tatagataac
    12781 ccataagcgc catacttaaa aatccgaatg tgatttgaaa ataagcagga ccaatcgcta
    12841 tcttgtataa gatgacatca agggctaata aaagagcaga ggtcgacaac aatcttgcat
    12901 tcatttcggg caatctaaaa acgtcttttg caatcaaagt gttcaaaata ttctctcctt
    12961 tgtcagttaa taataaaagg ataccataaa atcaccaatg tgtcatgaca cattggtgat
    13021 ttacatacac tggcatctaa caattaaaaa aagggtgaga agactgcttt tataaactgt
    13081 aaaaaagaga ttgtacgaat aaaaaaaata gaggcggaga atacttatcg tatcatccgc
    13141 ctttgttttt ttgttataaa aaacgtcaga ttagttatct gcttacatct tcatccatac
    13201 gggttctaat gtatttccgt ctagatcttg gacttctaat gaaaacattt cttcttcagg
    13261 cattcccata tctacttcgt ggaaagtacc accattttct tttgcaattt gagcaaattt
    13321 ttttacttca tccgcatttt caacactaaa cgaaatcaaa gcaccacttg tttttttatt
    13381 atcagctatt tctttatctt ttataaattt tttataaaaa tcatgagaga gaagcattat
    13441 ccaaaaagtg tcatcccata ccattgaacc agcttcttca gtagaaaatt cttcattttt
    13501 tttaaaaccc agcttttcat aaaaagcaac ggaacgattg acatcagcaa caggaaaatt
    13561 aacaaaaacc atactattca tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc
    13621 ttatttagga aacgctgtca aaaatttaga ttcatatata gaaaccaaaa taaaaatttg
    13681 catcatcaaa aaagtgaaga tgtgatagaa tgaggactgg ttcactttca cttaactaat
    13741 agatggaatt tctttataat aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa
    13801 agccggggaa tattccggta tgaaaggaat ggagagaaca atgaccatcc aatggtttcc
    13861 gggacatatg gcaaaagccc gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt
    13921 atttgaattg gtagacgcac gattaccttt atcttctcgt aatccaatgc tagatcagat
    13981 cattcagcaa aaacctcgcc ttgttttatt gaataaagcg gatctggcag atccacaaca
    14041 gacaaagctg tggcaagaat attttcgtaa acaaggccat cttgcattag ctatcacggc
    14101 acaagaaagt aaaggaatta aaggtctagt acctgcggct aaagaagcat tgaaagaaaa
    14161 acgggcaaga gatgctgctc gggggatcaa gcctcgcgct attcgggcaa tggtcttagg
    14221 tattccaaat gttgggaaat ctacgttgat gaatcgacta gtaggcaaga aaattgcaca
    14281 gacaggaaat aagcctggcg taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt
    14341 ggaattgtta gacacaccag ggattttatg gcccaaattt gaagatgaag aaatcggcaa
    14401 aaaactagcg ctgacaggcg cgatcaaaga ccagcttgtc catttagatg atttagcgat
    14461 ttacggatta gacttttttg cccgatatta ttcaggaaga atcagcgatc gttatggttt
    14521 atcaatcgag caagaagaaa aattggcacc ggaattactg atggatatta cagcaaaacg
    14581 tgggtataaa gaagactatg atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg
    14641 aaaacttgga cgatacacac tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg
    14701 aatccatcca aagtatcaaa gaacgattga aaagcataca gagaatagat gatcctacac
    14761 tagaatcatt aagaaatgat ccacgcaaag gcgtgcagca agcactggct tcttttgaac
    14821 gacgattgaa aaaggaagca ttaattaagg aaaaatatga agaaatgttt tgctttgaaa
    14881 agcaagggta cgcacaaggt caccaatgga ttgccggcat tgatgaagta ggaagaggac
    14941 cattagctgg acctgtcgtg gcagctgcag ttatcttgcc gaaaaatgag atcatattag
    15001 ggctgaatga ctccaaacaa ttatcagaga aaaagcgaga acttctttat caacaagttc
    15061 aagaaaaagc cctggcaatc gggatcggga tcgttgatca gcagacaatc gatgatgtga
    15121 atatttatga agcaagtaaa ttagcgatga tccaagcagt agaaaaattg tccattgtac
    15181 catgctattt gttgatcgat gcgatgacct tggatttgcc tattcagcag gaaaaaataa
    15241 tcaaagggga tgcacgatct gtgtcgattg ctgctgcaag tatcattgct aaagtatatc
    15301 gagaccaatt gatgaaagaa tatgacgtgc tttatcctgg atatggtttc tcgcataatg
    15361 caggttatgg aacaaaggaa cacttagaag gacttgaacg acaaggaatt acgccaatcc
    15421 atcgtaaaag ttttgcacct gtaaaaaaat atttacaata aaaaacagcc gcttttacgt
    15481 atcttattag aaagagagac gaaaggcggt tttatgatgt atcaaataga agaaaattta
    15541 ttgaaacttg cttatacaaa aggaatcagt agtcaaggaa aatgggcact tgctaactgg
    15601 atgatgcagt atccttataa agaaatcgga tttgaagaca tcattaagat aggtaaaata
    15661 accaaacacc gggagctgtt tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa
    15721 atccaatcga aacagtcgtt tatcacttgc tttgatccca aatatccccc tcagctgctt
    15781 cacttgacct atccgccaat tgtactgttt tataatggtg atctatcttt actttcttgt
    15841 aacatgttaa gcgttgttgg tggaagacac acttctgctt tagccaaaaa aacagtttat
    15901 tatttactaa aaccagtgat agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg
    15961 atagatacat acggtcacca agcagcaatc tctcatggtg gacggacaat cgcagtcatt
    16021 ggaacaggga tagagcaagt ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca
    16081 aatgatcatc ttcttttgag tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt
    16141 ccaatgagaa atcggattat cgcagctttg tctaaaggaa catgtgtctt tgaagccaag
    16201 aaaaaaagcg gctctctgat aacagcacag caagcattgg atcttggaag aacagttttt
    16261 tctgttccag gaaatatttt aacagaacat tctaccggat gtcatcattt gatccaagac
    16321 ggtgcagttt gcacggtttc gggtgaagat atcttggcag aatttaaaga ataacaccat
    16381 acttttcatt tgcttttctt gacaaacgtt ttctgaatgg atatgattag ctacgatttg
    16441 tatgaatcct cttttaatag taggaacacg acaatggaaa ggagtccatt cagtaatggc
    16501 gtataaatat ttagtgattg tagaatcacc tgcaaaagcc aagacaattg agaaatatct
    16561 tggacgaaac tataaagtaa tggccagtgt tggccatata agagatttac cgaaaagtaa
    16621 aatgggtatc gattttgaaa acaattatga accccattat atttctatac gcggaaaagg
    16681 cgatgtcatc aaaagcctga aagccgcagc aaaaaaagct caaaaagttt acttggcaag
    16741 tgacccggat agagaaggag aagcgattgc ttggcattta gcgtacctac ttgggttgga
    16801 tctgaaagaa aaaaatcggg tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc
    16861 ttttaaggaa ccaagaacga tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac
    16921 cttagacaga atcgttggtt attcgatcag tcctattctc tggcgtaagg tcaagaaagg
    16981 gttaagtgca ggacgtgtcc aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga
    17041 gatccgagaa tttgttccag aagaatattg gagcatcgac ggtaatttta aaaaagctcg
    17101 caagaaattc aaagcaaatt tctggggaat cgacggtaag aaaaagaaat taccagatgc
    17161 acaaagtgta aaagaagtca ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt
    17221 agaaaagaaa gagcgcaaaa gaaaccctgc gaatcctttc actacaagta gcctgcaaca
    17281 agaagctgcc cgtaagttaa actttcgaac aagaaaaacc atgatggtag cacagcaatt
    17341 atatgaagga atcactttgg gtaaacaagg aacagtcggc ttgattacat acatgcgtac
    17401 tgattctacc cggattgccg atacagcaaa agcagaggcc gcagcattta ttgaagatac
    17461 gtatggaaaa gaattttcta gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca
    17521 agatgcccat gaagctgtgc gtccaacgag tgtgcttcgt acaccagatg aactcaaaca
    17581 atatttagat aaagatcaat tgaaacttta tacattgatc tggtcacgtt ttgtggctag
    17641 tcagatgaca ccagctattt tagatacaat gaaagtgaca ttagaacaaa atggtgtcat
    17701 cttcattgcg aacggttcaa aaatcaaatt caaaggattc atgcaagtgt atgtggaagg
    17761 tagagatgac ggcaaagaag agaaagaaaa tattcttcca gaacttgaag aaggcgatgt
    17821 agtccaatct gttgatattg aaccaaaaca acattttact caaccaccag cacgttttag
    17881 tgaggctacg ttgattcgtg cactagaaga aaacggggta gggcgacctt ccacttatgc
    17941 accaacactt gatacgatcc aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga
    18001 gccgactgaa ctaggagaaa ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt
    18061 cgatatccat ttcacagcag aaatggaagg agatttggat aaaattgaag aaggcacgga
    18121 agcgtgggtc aaagtggttg atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc
    18181 ggaagaaaaa atcgaaaaaa ttcaaatcaa agatgaacca gcaggattcg attgtgatgt
    18241 ctgtggacat ccgatggtga taaaattagg gaaatatggg aagttttatg cttgcagtaa
    18301 ttttcctgat tgtcggaata cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt
    18361 atgtcatgaa ggacaagtca ttgaacgaaa atctaagaaa aacagactat tctatggttg
    18421 ttcccgtttc ccagaatgtg aattcacttc ttgggataaa ccagtgggca gaaattgtcc
    18481 aaaatgtgac ggatatctgg tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt
    18541 aaatggagat tacgaagaag acatccaaaa ataatagaaa agaggttctt tcattgaaaa
    18601 tggtaaatgt tgttggagca gggcttgccg gaagtgaagc tgcttggcag atcgcacaag
    18661 caggagtccc tgtccgttta tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca
    18721 caagtaattt tgctgaacta gtatgttcca attcattaag aggaaacagt ttggctaatg
    18781 cagtagggtt attgaaagaa gaaatgcgcc gattgaattc ggtagtaatc acttcggcag
    18841 atgaaacagc agtgccagct ggtggtgcct tagctgttga ccgcgattct ttttctgaaa
    18901 caattacccg aaaagtcaaa gagcatccat tagtaacagt gataaacgaa gaaattactg
    18961 aaataccgga aggaatcacc gtagtagcaa caggaccttt gacttctcat ccattagcag
    19021 aaagcatcaa agcattcaat ggttcagatg gtttttattt ctatgatgca gctgcaccaa
    19081 tcgtagacaa agctacgatc gatatggata aagtatattt gaagtcccgc tatgacaaag
    19141 gagaagcagc ttacttgaac tgtccgatga cagaagagga attcaaagct ttccatgagg
    19201 cactaatcaa tgccgaagtg gcagaactta aatcttttga gaaagaaaaa tattttgaag
    19261 gatgtatgcc aattgaagta atggcacagc gaggattcaa aacgatgctt ttcggaccta
    19321 tgaaaccagt tggattggaa gatcctaaaa ctggcaaacg tccttatgct gttatccagc
    19381 tgcgtcaaga caatgctgca gcttctttat acaacatcgt gggctttcag actcacttga
    19441 aatggggcga gcaaaaacgt gttttccgga tgattcctgg gttagaaaat gctgaattcg
    19501 ttcgctatgg tgtgatgcac cgaaacagct ttatgaattc gccagaattg ctgaaaccga
    19561 cttaccaatc aacgaaacgc gaagatctgt tctttgcagg tcagatgact ggtgtagaag
    19621 gctatgttga aagtgcagcg agcggactac ttgctggaat caacgccgca cgcttagcaa
    19681 aagaaatgga accagtcgag tttccacaag aaacagctat tggaagtatg gcttactaca
    19741 ttactcatgc ggaaggaaaa catttccaac cgatgaatgc caactatggt ctgtttcctg
    19801 aactgccaga gcgcattcga gacaaaaaag caagatatga agcaattgca caacgagctt
    19861 taaaagctaa tgaggcaata aaaacagaat tagaaaaaac tgcaatccca aatgagtaag
    19921 gaaactccat gaagagggtg agacggatag tctcgctctt tttcttgcgt ttttcttaag
    19981 aatttctcaa acaaaatgtg aggaattgtg ccaattgtga acaaacttag aaatattggt
    20041 taaattctga caatttattt gtctttttat aaacgctgtg ctaaattatt ctctgggaca
    20101 aaaggaaggt gagaaaatga cagaagccac gtgggaacaa aaatttttgc gttatttgat
    20161 tgtcgaaaga ggatattcag aaaaaacgaa agaagcatat gaagaagaca ttctccattt
    20221 caaacatttt ttggaggaat ctggagacgc tgatctgctg aaggcggaat attttacggt
    20281 tcgtgtctat ttgagtgcat tagctgatca tcgtcctagc tatagcagaa attctatcag
    20341 tcgcaagata gccagcctac gttcgtttta tcagtatctt ctcaaagaag aagtgataaa
    20401 agaaaatcca ttttcttacg ttcatctcaa aaagaaaaat ttgagactgc cacgtttttt
    20461 ttatgaaaat gaaatgcaag cactttttga cagcgtagca ggagatagtc ctttggattt
    20521 gaggaaccgc gctttactgg aagtccttta cgggtcaggc atccgtttaa gtgagtgtag
    20581 cagtttgtcg gtatcagaca ttgattttga ttcagaggtc atgttgatcc atggtaaagg
    20641 gaacaaagag agatatgctc ctctaggttc ttttgctcaa gatgctctgc aagaatattt
    20701 cagggaaggc cgccaggtat tgatgacaaa atatcacgaa gagcatgata tcgtttttgt
    20761 gaatcatcat ggaaaaccaa tcacgcctac tggaattgaa tatgtcttga atcaaatgat
    20821 caaaaaaagc agtttggata gtgatatcca tcctcacatg ctgcgccata catttgctac
    20881 tcatttatta aataatgggg cagatttgcg gacagtccaa gaattgctag gacatgccaa
    20941 tttatcgact acccagatct atgcccatgt aacaaaagaa agtttacaaa aaaactattg
    21001 ttctttccat ccaagagcgt aaaaataagg aaaagaacaa atttaggagg aaacaaacat
    21061 ggtcgaatca ccatttcatt caaccactat ctgtgccgtc gaaaaagacg ggaaatttgc
    21121 tatggctgga gatggccaag taacaatggg tgaatctgtt gtcatgaaag gcactgcaaa
    21181 gaaagtacga cgtatctaca atggagaagt tgttgtaggt ttcgccggaa gtgttgcaga
    21241 tgcgtttact ttagaagaaa aatttgaagg caaactaaac gaatataacg ggaatctaca
    21301 acgcgcagct gtcgaattag ctcaagaatg gcggacacag caatctatgc aaaaacttga
    21361 agcgatgctg atcgtaatga atgataaaga aatgcttctt gtttcaggta caggagaagt
    21421 tatcacacca gatgatggta tcttagctat cggatcagga gggaattttg cgttggctgc
    21481 agcccgagca atgaaaaact atgctcaaaa cgacatgagt gcgaaagaaa ttgcagaaaa
    21541 tgcgttaaat attgccgcag atatctgtat ctttactaat cataatatta tcgtagaaga
    21601 actgtaagag gtgagtagat atgtatgaat taaaacaaac accaagacaa atcgtaagtg
    21661 aattagatga atatatcgta ggacagcacg cagcaaaaaa atctgttgcc gttgctttgc
    21721 gtaaccgcta tcgccgtttg cagttagatg aaaaaatgca acaagatatt acaccaaaaa
    21781 atatgctgat gatcggacct acaggggtcg gtaaaacaga aatcgcacga cgtttggcaa
    21841 aaatcgtcaa tgcaccattt gtcaaagtcg aagcaacaaa atttactgaa gtcggttatg
    21901 tcggaagaga tgtagaatca atggttcggg atttagtaga aaatgcgatc caaatcgtag
    21961 aaaaagagca gtacagccgt gtatacacaa gtgctaaaaa aaatgcagaa aaacgtttgg
    22021 taaaaatctt ggtacctggt atcaaaaaag aacagaaaca atccactaat caatttgaac
    22081 aaatgatgaa tctttttaat gctagtcagc aaaataccgc tgaaccgcaa gaagaagtga
    22141 cagaagatat tcgaatcaat cgccgtacag tcttggaaca actacaaaag ggtttactgg
    22201 atagtcgtga agtaaccatt gaagttgaag accaaaagaa acctgcacca atgatgaaca
    22261 atggattgga gcaaatgggg atcgacctaa atgaaactct aggtgctttg acaccgaaga
    22321 aaaaagtgga acggacggta acagtcaaag aagctttaga gttattgatc aaggaagaat
    22381 cagcaaaatt agtcaaggat gctgatatcc atagtgaggc aatcaaatta gcggaagaaa
    22441 gtggaattat ttttatcgat gaaatcgata agatcacttc taaatcacag caatctggag
    22501 aagtatccag agaaggcgtc caaagagaca tattgccaat tgtcgaaggt tcacaagtaa
    22561 acacaaaata cgggatcatc caaacagatc acgtcctatt cattgcttca ggagctttcc
    22621 atttgtctaa accaagtgat ctaattccag aacttcaagg acgcttcccg atacgggtag
    22681 aattagatga tttgacagct gaagattttg tgaagattct gaccgaacca aataatgctt
    22741 tagtaaaaca atacgtcgcg ttgatcggta cagaaaatgt gtcagttacg tttacaaaag
    22801 aagcaattga acggatcgct caaatcgctt tcgatgtcaa ccgtgacaca gataatatcg
    22861 gcgcacgcag actgcatacc attttggaaa aactactaga agatctatta tttgaagcac
    22921 cagatatgca aatgggagaa ataacgatca cagaagcata tgtggatgac aaacttggct
    22981 caattgcggc aaatgaagat ttaagtcgct atattttata aaaaatcgga ggacgacaat
    23041 gacagacttg ctaacgaaaa cccgcaagat aaacacactt ttgcagcaaa agaatacttt
    23101 tgacatgcaa gcagatttgc cttacgataa aatggctgtc acactaggtg gtattcttga
    23161 cagcaacacc tatatcatag gaagtgacgg agtattacta ggttataatg aacgtcacga
    23221 tgtgaacaat gaacgaatca aaacaatgtt tgttgagaaa caatttcctg aatcttatac
    23281 tgatacagtg gcacagttga tgaaaactga agctaatatc ccaattacta gtgatattac
    23341 tgcatttccg gtagagtcaa gagagaaata tccatttggt ctgacgactg tcgttccgat
    23401 ctttggagct ggcgaacgtt tgggtacgat tatcttatct cgtatggaac aatcgtttgc
    23461 agatgacgat ctagtcttag cagaatatgg agctacggtt gtcggtatgc agatccttta
    23521 tcagaagtct cgaagcatcg aagcagatgt acggagtgca acggctgtcc aaatggcaat
    23581 caacacatta tcttatagcg aattgaaggc tgtacaagct atctttgaag cattggatgg
    23641 agatgaaggt cgtctgactg cttctaatat tgccgatagt atcggcatca ctcgttctgt
    23701 gatcgtcaat gctttacgaa aattggaatc agctggaatc attgaatctc gttcgttagg
    23761 catgaaggga acttatttaa aagtattgaa ttcccgtttt aaagacgagt tagcaaaaca
    23821 cagttattag aagtaagggg actggggcag atagaaatgt ttgttccagc cttcccattt
    23881 taaaaagtaa gtcgaagttg gaggaatggc aatgactgtt attattcaaa atgaccagct
    23941 gatcgccgaa atcgctgaac acggtgcgga attgatcagt ctgaaatcaa aagaacatgg
    24001 tttagagtat atctggcaag gtgatccagc gtattggggc agacatgcac cggttttatt
    24061 cccttttgtt ggacgtttaa aagatgatca gtatgtttat caagggaaaa catatcaaat
    24121 ggggcaacac ggatttgctc gggatatgga ttttgaagtc attgaacatg gacaagaaat
    24181 ggctagtttc atcttaagga gtacgccgga gacattagaa aagtatccat ttgattttga
    24241 attagtgatc agctatgaat taggaggaga cggcatcacg gttcattatc aagtggcaaa
    24301 taccggtgaa aaagaaatgt tcttttctat cggtggacat cctgctttca atgtacctct
    24361 tgaagaagga ttgtcatttg aagatttcta tctggcattt tctccattga aatcaagatt
    24421 acgcctacca ttgaaaggcc cattcatcga tatggatcaa aagacgttag gtcagacaaa
    24481 tacgaatctg gctttgaatc gtgaactttt tgagcaagat gccttggttt ttgaaacaag
    24541 gggtctaaat gcgtacgcca ttcgaagtga aaaatcttcc catagcgtaa ctttaagcta
    24601 taaagatatg ccttatgtag ggatctggtc tccttatcca aaagaagcgc ctttcgtttg
    24661 tatcgaaccg tggtgcggaa tcgctgacac ggtgaacagt agtggaaact tgatggaaaa
    24721 agtagggatc aatcaactag gtgcccatga aatcttcaag acaaaatatt ccatcacagt
    24781 gaagtaagag gttgtgaccg agaagttagc agggaacacc gtttaccaag gtgtgtaaaa
    24841 aagctatcct gtaagtaata agggagtgtg acatcgattt gtcacactcc cgttttatct
    24901 ctttggaatg aaaaagtatc aggatttttt cttgctgcta ttcaaaccaa acgggattcg
    24961 actttcagta tggtttctga tacgcttgat attgtcttta tgccgataaa agataaagga
    25021 agaaactaaa aaggcaatga tcgtcagtaa ccaattgggc ttagccaaaa tcacaggagc
    25081 tacataagga aggacgatcg tagataaagt gatcaatact gcactgatca tactgctcaa
    25141 actcaccata cttgtacaaa ataaactaac gataaagatg atacaagaat aaacaaaaaa
    25201 tacaggatta aatcctaaaa gcattcctgc acttgtcgca acagcttttc ctcctttgaa
    25261 cccagcaaaa atcggaaatg tatggccaag aatggcgcat accccaaacc aaagagggtt
    25321 aatcccagaa aggtggaaga tcatcggcaa acaagtagcc aatgtacctt ttaaaatatc
    25381 cataagcagg acaacaatcc cggctgtttt gcctaataca cgaaatgtat tggtcgttcc
    25441 agtattaccg ctcccgtgtt gtcggatatc ttttttgaaa aacaatttgc caatccagac
    25501 accggaagga atagaaccta aaagatatgc taaaataagt aataagatca atttcatttt
    25561 ttaccctctt tcaagttgaa aactatcctt tattttagca tgaataggta acaggaacaa
    25621 ttgaaaaaat gaaagataca agtataatag atggaataaa aagaaaagca aggggtggca
    25681 gaaatgtctg taaaaaatcc aacacaagaa acgattttca actatttgga agaagcaaaa
    25741 aatattgccg tcgtaggctt gagcaataaa ccagaacgaa caagttacat ggttgcacag
    25801 gtattgcaag acaatggata taaaatcata ccagtaaatc cattattggc tggtgaagaa
    25861 attctaggcg agaaagtcta tgcgaatttg acagaaatcg atgatccaat cgatatcgta
    25921 gatgtattta gacggagtga atttttacct gaaatagcag gtgatttttt gaaaactgat
    25981 gctaaagtgt tctgggcaca attagggatt gaaaacaaag aagcagctga gatgctaaga
    26041 aaagcaggac gtacagatat cgtaatggat cgttgtatta agattgaact tggtaagttg
    26101 aaagaaaacc aaaaataaaa atgaccacaa agaagagatg atcgatagag gggaatgtcg
    26161 aaaaagcggt taggagataa ttaaaattga tttctcgtct accctctttt ttttcacgac
    26221 cttttctagt atgataatcg tggtgcggtt ttgccgcaaa gtattattga aggagcgttt
    26281 gccttggcaa aaaaagtaaa caacgaatac aatgactcgt ccatccaagt attggaagga
    26341 ttagaggcag ttagaaaaag accagggatg tacattggat ccactgacag ccgaggcttg
    26401 catcatttag tctatgaaat cgtcgataat gcagttgacg aagcattatc aggttatggc
    26461 agtgagattg atgtgaccat ccatgaagac aatagtataa ctgttgcaga tagcggacgt
    26521 gggatgcctg taggaatgca tgcttccgga ataccaacag ttgaagtgat ttttactgtg
    26581 ctgcatgctg gcggaaagtt tggtcaagga gggtacaaaa cttctggtgg actgcacggt
    26641 gtaggagcca gtgtggtaaa tgccttatcg aaatggctaa ttgtaacgat cgttagagat
    26701 ggcgtagagt atcagcagaa atttaaaaac ggcggtaaac ctgacggtac tttgaaaaag
    26761 ataggaaaaa caaaaaaagc aaacgggacg actgtccatt ttttaccaga tgatacaata
    26821 ttcagtacaa cgaaattttc atatgaaatt ttagccgaac ggttaagaga atctgccttt
    26881 cttctaaaag gcgttaaaat ctctctttct gatcttcgtg gagaagaacc agtcaaggaa
    26941 atttttcatt acgaagaagg aatcaaagaa tttgttgatt atctgaatga agaaaaagat
    27001 acattgacac cagttgttta tttttcagga gaaaaagaag gtatcgaagt ag
//
LOCUS       DACCQI010000030        26611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCQI010000030 DACCQI010000000
VERSION     DACCQI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.030"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(57..974)
                     /locus_tag="IVO76_000982"
     CDS             complement(57..974)
                     /locus_tag="IVO76_000982"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7428960.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            1176..1613
                     /gene="zapA"
                     /locus_tag="IVO76_000983"
     CDS             1176..1613
                     /gene="zapA"
                     /locus_tag="IVO76_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7428961.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            1671..2219
                     /locus_tag="IVO76_000984"
     CDS             1671..2219
                     /locus_tag="IVO76_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7428962.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            2334..4694
                     /locus_tag="IVO76_000985"
     CDS             2334..4694
                     /locus_tag="IVO76_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7428963.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            4781..5095
                     /gene="trxA"
                     /locus_tag="IVO76_000986"
     CDS             4781..5095
                     /gene="trxA"
                     /locus_tag="IVO76_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7428964.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            5277..7055
                     /gene="uvrC"
                     /locus_tag="IVO76_000987"
     CDS             5277..7055
                     /gene="uvrC"
                     /locus_tag="IVO76_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7428965.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            7200..8099
                     /locus_tag="IVO76_000988"
     CDS             7200..8099
                     /locus_tag="IVO76_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7428966.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            8092..9354
                     /locus_tag="IVO76_000989"
     CDS             8092..9354
                     /locus_tag="IVO76_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7428967.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            9515..11413
                     /locus_tag="IVO76_000990"
     CDS             9515..11413
                     /locus_tag="IVO76_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP7428968.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(11712..12431)
                     /locus_tag="IVO76_000991"
     CDS             complement(11712..12431)
                     /locus_tag="IVO76_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP7428969.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            12768..13100
                     /locus_tag="IVO76_000992"
     CDS             12768..13100
                     /locus_tag="IVO76_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428970.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            13087..15090
                     /locus_tag="IVO76_000993"
     CDS             13087..15090
                     /locus_tag="IVO76_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7428971.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            15109..15579
                     /locus_tag="IVO76_000994"
     CDS             15109..15579
                     /locus_tag="IVO76_000994"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7428972.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            15600..16184
                     /locus_tag="IVO76_000995"
     CDS             15600..16184
                     /locus_tag="IVO76_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7428973.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANKLFE"
     gene            16199..17185
                     /locus_tag="IVO76_000996"
     CDS             16199..17185
                     /locus_tag="IVO76_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7428974.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            17175..17486
                     /locus_tag="IVO76_000997"
     CDS             17175..17486
                     /locus_tag="IVO76_000997"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7428975.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            17508..19289
                     /locus_tag="IVO76_000998"
     CDS             17508..19289
                     /locus_tag="IVO76_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7428976.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            19282..20658
                     /locus_tag="IVO76_000999"
     CDS             19282..20658
                     /locus_tag="IVO76_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7428977.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            20665..21300
                     /locus_tag="IVO76_001000"
     CDS             20665..21300
                     /locus_tag="IVO76_001000"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7428978.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            21330..22685
                     /locus_tag="IVO76_001001"
     CDS             21330..22685
                     /locus_tag="IVO76_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7428979.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKITIHSKIALTVTGTLLLGGFVVFFFTERNGETLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(22877..24271)
                     /locus_tag="IVO76_001002"
     CDS             complement(22877..24271)
                     /locus_tag="IVO76_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7428980.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQKLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQNKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            24363..25334
                     /locus_tag="IVO76_001003"
     CDS             24363..25334
                     /locus_tag="IVO76_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7428981.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKDKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSATLTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            25331..26112
                     /locus_tag="IVO76_001004"
                     /pseudo
     CDS             25331..26112
                     /locus_tag="IVO76_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            complement(26281..>26611)
                     /locus_tag="IVO76_001005"
     CDS             complement(26281..>26611)
                     /locus_tag="IVO76_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7428982.1"
                     /translation="ASIEFYQKAFGFEESRRRDFPENKFTLVYLTLPGDDYELELTYN
                     YDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPGTTPSYYFVIDPDGY
                     KIEVIRG"
BASE COUNT         8730 a         4527 c         5752 g         7602 t
ORIGIN      
        1 gagaacaccg tttattcgga acaagggact gtgacatgac ttttgtcaca gttccattat
       61 ttttttagca gcttcaaagc tttttctgta ttggcaaaat gaagtttggc atatttttca
      121 agcataggca tgccgccttt ctccaatagt tttgccgcta cttggtcaga tttcgttcca
      181 gctcctgatg gaagggtgat tccagcttca gctgatgctt tgtgtaactc ttccaaaaag
      241 ctggcacgac tgatgataga agcagcagca acagctaaat gatattgttc tcctttagta
      301 acgaagtaca gtttttcttg taattgattt ttttctaaac gcgcatattt tttaaaattt
      361 gcttccggcg taaactgatc gatcaaaatc gcttctggtt tttctggctc gatttttttt
      421 agcagcaaat ggatcgcttg attgtgcaaa gcgactttca tccgaacggc attgtatttt
      481 ggttggatct cattgtattt ttctggagat aagacaagca gtttataggg gatggttttt
      541 tttaagatgt cagcaagctg gatgatttgg ctatctttta actcttttga atctctaaca
      601 ccaagtttac gtaattctgc aagctgatca cgtttcacat aagcagcaca tactgttagc
      661 ggaccaaaat agctgccatt tccgacctca tcactaccta aaacggataa ttgactgata
      721 ttttttggca aagaagaggc tgtttttgct ggcttggctt gtttatcctg tgcctccccc
      781 catttatcag cttctattgc tgcattcgta ccttgaaaca tcacttttcc ggatgtataa
      841 gcggtaatgg tcgtcccgtc tttttttgca gcaaagctag tataaggaat ctgtttattt
      901 aacaaataag agcgatagta tgctttcatt tgactaagtt ggtcgtttgt tgctttgata
      961 atgacgttgc tcataaaatt ctcctcgttt ttatacataa aactataccg caactcacga
     1021 cttttcgttt tctaagagat tgcgagacat agcatttttt taaagtttat catataatag
     1081 ttaagtatgt gaaaaatatt tgtggagaac ggtgaaagaa ccaacttcat ggtacactaa
     1141 ataaaacttt catcaaatag gaggaaatga ttcgtatggc gcacgaaaaa acacgctaca
     1201 aagcagtgat tgccaaccaa acatatacta ttatcggacg ggaaaccaag catcatatgg
     1261 atatcgtaac gaagctgatc aatgaacagt tagcagaatt gaaacaatta tcaccacaaa
     1321 tggataatga acaagcagca atcttgatgg ctgtcaatgc gctttcggat caattgaaga
     1381 aacaagaaag aatcttggag ctggaagaag aaacagcaga attaaagaaa aaaatgatca
     1441 aatttacaga gcttgaaaat cgtgtgaaac gcattgaagc aatcgagaac gaagctcggg
     1501 aagtattgaa agaaaatggt caagcagatc ataccattaa aaatcatgtg gaagcacaac
     1561 agattttaaa cgagaaacga aaaggacaaa ttaaacaaaa atcttctaat taagatcctt
     1621 ttatcaaaat aagaaaaatt taaaaaagtt agaagagaaa ggaacgtagc atgctgagtt
     1681 tgctgatttt atttttattg ctaattgcgt ttttttctgg agcacgtcgc gggtttgctt
     1741 tgcaaatcat attcgcgatt ggatatgtat tgtcatttat tgctgcccag catttttaca
     1801 aaccattagc aagtcatttg gaattataca ttccataccc agctgtcacc ccaacatcaa
     1861 agttagcatt ttttgatcag gtatttgctt ttcacctaga tgaagctttt tatgcaggaa
     1921 ctgcttttct gattattttg ctaattggct ggctgcttac ccgatttgtg ggggtttttg
     1981 tccatggatt gacgtatgtg ccgatcttga gacaggtaga ctggattgca ggaggcatcc
     2041 tgtcactaat catggcttac gttactatat ttttgatttt gcagttactt gcctttgttc
     2101 cattagatgt ggtccaaaat caattcggcg agcatagttt cgctcgtttt atcgttgaga
     2161 gaacgccatt tcttaccaac aagatgtatg agctttgggt aacgaatatt ttgaattaag
     2221 gaaaatagtg ctggaaaata tttcgttttt tgtacagagt agtttattta agaaggctgc
     2281 gtagcgtatg tccggcacag ctcttttttc atagtgaaag ataaggtgat aaaatgaata
     2341 accgaatcct tgaaacattg gaatttgaaa aagtcaaaca gatggtcaga caatttgtag
     2401 tgactgcaca aggaaaagaa gaactggcag aattggtacc agtgagtgag aaacaaacga
     2461 tcacaaactg gcttcaagaa actgaggatg gattgaaagt gcaacgtctg cgaggcggga
     2521 tccctatacc taaacttgaa aatatccgtc cccatatgaa acggatcgag atcggagcag
     2581 acttgaatgg agtggagctt gcacaagttt ccagagtatt gtctacgaca agcgaattga
     2641 atcgtttcat tgatgatctt tctgacagtg aaatcgaatt tgcacgtttg tatatgtggg
     2701 cagatcagtt agtcacgatc cctgttttga gccgccgttt aaaggaagca atcgatgaag
     2761 acggacgcgt gactgatgac gcttctcctg agttaaagag cataagacag aacattcggc
     2821 gaagtgaaca agctgttcgt gaacagttag atgggatcgt ccgtgggaaa aatgcaaagt
     2881 atttaagtga tgcgatcatc accatgcgaa atgaccgcta cgttatccca gtcaaacaag
     2941 aataccgcgg tgtatttggc ggtgtagtcc acgatcaaag tgcttcagga cagactttat
     3001 ttattgaacc aaaacaagtg gtggatttga ataatcgttt gcgtcaatac caaatcgctg
     3061 aacgaaatga gattcaacgt attttaagtg aactttctgc tgaacttgtt ccccatcgtc
     3121 aagaaatcat ccacaatgct tatgtgattg gtaaaatgga tctgatgaat gcaaaagcac
     3181 gatttggtaa agaagtaaaa gccatcgtgc cgggaatcag tgaagacaat catgtcgtct
     3241 tgaaacaagc aagacatcca ctgatcgatc aggaaaaagt agtttctaat gacatcacaa
     3301 tcggaaaaga ctatcaagcc atcgtcatta caggacctaa cacaggtggg aaaacgatca
     3361 cgttgaagac tttaggtctg ttacaactga tggggcaagc aggattaccg attcctgcag
     3421 gtgaagaaag tcaaatcggt atttttgagg aagtatttgc ggatatagga gacgaacagt
     3481 cgattgagca aagtctatcg accttttctt ctcatatgac gaacactgta gatattttat
     3541 caaaagtcaa tgaaaaaagt ctggtattat ttgacgagct cggtgcagga acagatccgc
     3601 aagaaggtgc tgctctagca atcgcaatct tagatgatct tggtaaaaag tctgcttatg
     3661 tgatggcaac aacgcattat cctgaattga aagtttatgg atacaatcgg gcaaatacga
     3721 ttaatgccag catggagttc gatgtggata cactaagtcc aacgtatcgt ttgctgattg
     3781 gcgttcctgg acgaagcaat gcgtttgaaa tctctagccg attaggtctt gatacagaag
     3841 ttattgatga agcgaagcag ttgatgaatg atgagagcca agatttaaac gaaatgatca
     3901 cagatttaga gaatcgccgg aaaatggcag agaccgaata tctggaaatg cgtcattttg
     3961 tttctgaggc acaagaactt cacgatgatt tgaaagaagc ttacagctat ttctttgaag
     4021 aacgtgaaaa agaaatggaa aaagcaaaga aaaaagcaaa cgaagtcgtg tcagaggcgg
     4081 aagaaaaagc agaaaaaatc attgctgata ttcgtaaaat gcaacagcag attgggcaag
     4141 gaaatgtgaa agagcatcag ttgatcgatg caaagaccca attagcaaat ctacatcaag
     4201 aagaaacttt gaagaaaaat aaagtattga agaaggcgaa agaacaaaaa acgttgaaac
     4261 cgggagacga agtgttggtt acgacttacg gccaaagagg gacgctttta cgtaaaaacg
     4321 ggaatcagtg gcaagtagaa atcggtatat taaaaatgaa tgtgtccgaa gacgagctga
     4381 cgcctgttgc tcctcaaaaa gagccgacac aacgagttat ccatgctgta cgatctgaat
     4441 ctagtagtca cgtgccgaat caattggacc ttcgaggaaa acgatacgaa gaagcgctaa
     4501 gtgaggtaga tcaatatttg gattctgcca ttctagctgg ttatcctcaa gtgactattg
     4561 tccacggaaa aggaacaggc gcacttcgca agggaattac tgattatctt aaaaaccatc
     4621 ggagcgtcaa aagctttgag tttgcaccgg caaaccaagg cggaaatggt gcgacaattg
     4681 ttaaatttaa gtaaagctta ctaaaggtaa gcagatggtt cgctatccaa gtatagaaat
     4741 gctatactaa tcatagattt taaaaatgga ggtctttgat atgtcacaag taattacaga
     4801 taagacattt aatgaagaaa cagataaagg acttgtattg attgattttt gggcaacttg
     4861 gtgtggtcca tgtcgtatgc aagcaccgat tttggatcag ttggaacaag aatatgatga
     4921 agaagaattc agaattgcta aaatggatgt ggatgaaaat ccagaaactc ctcaacaatt
     4981 cggtatcatg agcattccaa cattaatgtt gaaaaaagat ggtcaagtag tagaaaaagc
     5041 tgttggtgtt cattcaaaag aacaactacg tcaaatgatc gatcaatatt tataaaaact
     5101 tacagcaaaa ataagctgag ggtgggactg aatccagttc cgtcctcggt ttttttgcgg
     5161 ttgagttaag gttattcaaa ttaatatttt ttgagtataa ttacgttgtc aggctggatt
     5221 gtttttttga cagacatgct ataataactt taattgaata tgaaggggag taccagatga
     5281 acgaacgaat caaaaacaaa ttagctttac ttcctgatca gcccggctgt tacttgatga
     5341 aagataaaaa tggaacgatc atttatgtcg gcaaagccaa aatattaaaa aatcgtgtcc
     5401 gctcatattt tactggcagc cataatacga aaacggaacg gcttgttagt gaaatcgaag
     5461 actttgaata catcgttaca gaatcgaata tcgaagcatt gctcttagag atcaatctga
     5521 taaagaaaaa tgatcctaaa tacaacatta tgttaaagga tgataaaacc tatcctttcc
     5581 ttaagataac gaatgaaaaa tatccccgtt tggtgattac tcgtaaagta ctaaaagaca
     5641 aagcactgta ttttggacca tatcccgatg tcggtgcggc gaatgagaca aagaagatcc
     5701 tcgatcgatt atttcctctg agaaaatgca aacctagtca gacgaaggag ccttgtcttt
     5761 actatcattt gggacaatgt ctgtgtcctt attatttcga cgtagatccg aaagtctata
     5821 cgaaaatcgt tgatgaagtg aaaaagtttt taaacggagg acacgaaaaa atcgagtcag
     5881 agatccatca gaaaatggaa aaagctgctg aaaacatgga gtttgaaaaa gctgcagaat
     5941 accgagacca gatccgtgcc attgagacaa tcatgactcg ccaaaaaatg acaaatactg
     6001 atttactgga tcgagatgta tttggttatg cgattgacaa aggatggatg tgtgtccaag
     6061 tgtttttcgt aagacaagga aagctaattg aacgagatgt ttcaatgttt cctttctata
     6121 acgaagcaga agaagatttc ttgacttacc tcggtcagtt ttatcaggaa aatgagcatt
     6181 tcattcctaa agaagtcttg attcctgata cgatcgacaa gcccagtgta gaagctttgt
     6241 tagctacgaa agtgctgcaa ccaaaacgag gagaaaagaa aaaattagtt gaattggcga
     6301 ataagaatgc ctcagttgct ttgaatgaac ggtttgatct gattgccaga aaacaggaac
     6361 ggacgatcgg agcagttgaa cgtttaggaa acgcgatgaa tatcccgacc ccaatacgta
     6421 tcgagtcttt tgataactct aatatcatgg ggacgaatcc tgtggctgcg atggtcgtat
     6481 tcattgatgg aaaaccggca agaaaagaat atcggaaata taaaataaaa acagtcaacg
     6541 gaccggatga ttatgcatca atgagagaag tcatctatcg cagatattca cgagtgattc
     6601 gcgaagggtt gcctttacct gatttgatct tgatcgatgg aggaaaagga caagtagatg
     6661 tggcaaaaga tgtcttagaa aatcaattag gaatcgatat tcctattgct ggcatggcaa
     6721 aaaacgataa acataaaaca aatgagttgc tattcggtat agatttggct gtagtaccgc
     6781 ttgagcgaaa ttcaccagag tttttcttac tccagcgaat ccaagatgaa gtccatcgat
     6841 tcgcaatcac tttccatcgg cagctacgca gtaaaaacag ttttgcttcc cgattagatg
     6901 aaattgaagg gttaggacca aaacgaaaaa aaatgctgct taaagagttc aaatcgttga
     6961 aaaatatcac agcagccacg acagaagaat tacaagaaat cggactgccg aaaaatgtcg
     7021 cacaaaacgt atttgataaa ttacatcagg aatgaggaaa gggcacttta caaagaaagt
     7081 aaagtgtcct atcattttat gtacaacaaa aagagcgttt acgttttgtt ggtaaataat
     7141 aaatcgctta cgtctggtgt ctaaggatat atgtgatttc ttgaagaagg agtgaagaaa
     7201 tgttagaagt caaggatctt gttaaaactt ttgggaacta cacagctgtc gatcatgtct
     7261 ctttccaaat ccccgatgga aaaatcatgg ggctaattgg ccaaaacggg gctgggaaga
     7321 caacgacttt tcgtttgatc ttggattttt tgacacaaga ccaaggagaa gtgttatgga
     7381 atggccatca tcttggggag aaagattatg atgtcatcgg ttatcttccg gaggaacggg
     7441 gactttatcc gaaagtaacg attcaagacc aactgcttta ttttgcagaa cttagaggga
     7501 aatcaagaaa agagattgaa ccaaaaatcg atttttggat ggaaaaattt caggtaaaag
     7561 ggaaaaagac ggataaagta aaatctttgt ctaaaggaaa tcagcaaaaa gtccagctga
     7621 tcgccacgtt aatccatgaa cctaaattga tcattttgga tgagccattc agcgggttag
     7681 atccggtaaa tgctgaattg ctaaaagatg gaatattgga attgaaaaaa catggttcat
     7741 gtgtcatttt ttctagccac aatatggata atgtagaaaa aatctgcgat catttgatca
     7801 tgttgcgaaa cggaaaaatg gttttgaatg gaaaagtaca tgagattcgt gaatcatttg
     7861 gccgtacgaa gttattcttg gaatctggtt taagccaaca ggaagtagaa gaaatcgaag
     7921 gcgtgaaaaa agtgatcctt catgaagaca atacgttaga gattacctta gaagatcctg
     7981 aagctgggaa agaaatcttt actcgtgcga ctcagttcgg ctacattcct atgtttaacc
     8041 agcaaccgcc aactttggaa gagattttca aattgaaagc aggtgaaatc gatgcgtaag
     8101 ttttggatta ttaccaaaga tgtctattta aaaaatgtta agtcgatttc tttcttgatc
     8161 atgatattag tcccttttat catgctgggt atcatttatg tagcaggaaa ctttgctcaa
     8221 caaaatagtg atgtcaataa aatcggcgta ttggctgata atgagcaaat cagtcagcaa
     8281 atgagccaag tcaaaacaga tgattttgag ttcaaagcag ttgattctga agaaaaagca
     8341 caaaaagagc tttcggaaga aaaaatcgat gcgtatttag tagtctcaac tgagaatgat
     8401 aagataaatg ggatgcttta tagcgaaaat tcattaggac aaagtacaca actactgata
     8461 caacaacaac tgactggctt gcaatcgatg cttcgagcaa gtcaattagg catttcacca
     8521 gaagaagtag ctagcttgag ccaacaagca agtttgactc gacaaaaagt caattttgat
     8581 tcaaaaggaa aaatgacagt tggagaagat aactcgacag tccaatatgt cgtaagttat
     8641 gtagcaacga ttgtcttgtt cattattatt ttgacgtatg cgcagatcat tgcacaagag
     8701 atcgcttctg aaaaaggcac gcggatcatg gaagtgatcc tgtccagtac gactgcacaa
     8761 aagcattttt acggaaaact aacaggtgtt ttacttgttg cattaacgca aatggcatta
     8821 tacggtatta tttttggtat aggatacagt caattcaaag atatggaaat ggtcaaaaat
     8881 atattagacg gtatctcatt ggataacttg attggaccat tcttgtggta ttctcttcta
     8941 ttcatgatct ttggcatttt gattttttct gttttagcag ctttatgtgg ctctttggtc
     9001 aacaaagcag aagatacagc aaaagcaatt ttgccagtga cttatctatc tctaggcgga
     9061 tatatgcttg gattgatact aggagcttca gatccaaaca atatcgttat tcggatcact
     9121 tcgtatattc cattcttatc ttcatatatt atgccggttc gcttagcaaa tgagacagtc
     9181 ggtatgggtg gagcattgat ttccttgttg gtacttgtag ccgtaacatt cttattgatg
     9241 atcttgtctg caaacatgta caaatcgaat gtactcgtgt atagcgaagg cggattatgg
     9301 agttcattga aacaatcgat ttcaattatg agaaatgaac gaaaaaaagc ataaaatata
     9361 gaaaaatata gaaggaagct ggacatttat tgttcccagc ttcttttttg tatagaccta
     9421 tttctatttt tttattatat tggatatata ttcattgaaa atagagcata acaactttat
     9481 aatgttgagt ataccaattt ttggaggcgt tttaatggac cgctatttag atttactttc
     9541 agagaagttc cctacaacgg aagatgtcat cacggaaatc atcaatctcg aagcaatttt
     9601 gcagcttcca aaaggaacag agttattttt gagcgatata catggcgaat ttcctgcttt
     9661 tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa aagatcaaag accttttctc
     9721 tgaacaaatg acggaaacgg agatcagcca attcactatt tttacggctt atccagaata
     9781 tgtgttgacg actgattggt acaaaaaaca agataaatcc cagctgatcc atcgtttgat
     9841 cgatctttta cggtttacga cagtcaaata cactcgatca aaagttcgaa aaggattgcc
     9901 gaaagaatat agctatatca ttgaggaact cttgtatatc gatgatcgta tcaacgggaa
     9961 aaaagactat gtaaaaaaaa tcattgaaca acttgtcttg atgaaggaag aagaaaaatt
    10021 tttaacaaaa cttgcgcaga cgatccaaaa atctgtcatc gatcatttgc atatcgtggg
    10081 tgatattttt gatcgaggaa ctcaagcagc caaagtaatg gatcgaatca tggatttttc
    10141 ctctgtagat atccagtggg ggaatcatga tattttgtgg attggtgcgt attgcggctc
    10201 tgaggcatgt ttattgacgc ttttacggat tgccgcacgc tacaactatt tatttgaatt
    10261 agagaaagaa tatggattaa atttacgtcc attattttct tttgcccatc aaacctatca
    10321 gaagaatcca gtatttaccc ctaaaaacag gaaagatcta tctgaaagag agcaagaaga
    10381 attagaaaag atccatcaag cactttctat cctccaattc aaatcagaac atcagttgtt
    10441 gaatcgacga cctgaattta aaatggacga tcgtaaattg ttggaaaaaa tcaactatga
    10501 ggaatcaacg atcgttttag aaggtcagct ctatgagttg aaagggactt gttttcaaac
    10561 gatccagcca gatgatccta aaaaattctt ggaagaggaa caaaaagtga tcgatagttt
    10621 gatgtattca ttccagcact cgttaagaat gcaaaaacac atgacgtttt tgatcgaaaa
    10681 agggggaatg tatctcacga acaatcagca tgtactgttc catggctgta tacctgtaga
    10741 tgaaaagggg cagctcttag catttgaatt agaccaatct tatcgcggaa aagaactgct
    10801 gggatttttt gaaaaacaga taactgaaag cgcaaaagat ttgactgcaa atgaagattt
    10861 ggcgaccgat ttgatttggt atgcgtggag cgggccattt tcgcctttat tcggcaagag
    10921 taaaatggca acttttgagc gctattttct cacagagcct catacgcacg tcgaaaaaag
    10981 caatccctat tatcacttgc gagatgaaga gtggttcagt gaaaaaattt tggcagaatt
    11041 cgatgcaaaa gaagaaggtt cagcaatcat caatggacac acgccagtaa aagtaaaaaa
    11101 aggagagtca ccgatcaaag caaacggaaa agcatttgtt attgacggag gtctatccaa
    11161 agcttatcaa aaaacaaccg ggattgctgg ctactcatta ctatgcaatt cttacggttt
    11221 ccaaatcgtg acacattatc cgttcttgcc tattgaacaa caatttgcaa aaaaatctga
    11281 tcaaacgaat gtaaaaaaag tgatcgagca gcagctccca cgcaaactta atcggaatac
    11341 gacaaaagga atggaattac agcaacagat tactcaactc caaaggctgt tggattatcg
    11401 aaaggacgat tgagaaaaat gctagccaac attgctcaat agactaaaaa gcgactgcaa
    11461 caaaaggcgt gtcgcagtct ccttcggaat tagctcaaaa aacggtaaaa tattagaagc
    11521 tatttttctg tttatttctc taatactcag gtcagggcgc tgatttcacg acctcttcct
    11581 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccaac aaacatgaga
    11641 agctgttttt tgaaattgct ccttgtgact cagagttgaa cggcaggctc acgacctaat
    11701 cagaaaaacg attaaaattc attcacaaag aaatcgaata aacgctgttc agatgaatct
    11761 acacgatgag aaagttccgg atgccattgg acaccaagga ttttgtgcat attatcttta
    11821 ctttggactg cttcaatgag tccatcgttg gaaaaggcga tggcttttaa caaaggagaa
    11881 agatctttga ctgcctgatg atgataggaa ttcacaaagt atgaatcagg gagaaggtca
    11941 cttaataaac tgtccgaaac gattttgact tcgtgagttg caaactgcgg ggcagtgggc
    12001 tgttgttcgt gttttacgga ccattttgga tataatgaaa gatcttgata aagtgttccc
    12061 tccaacacga cattcaatag ttgaagtcct cggcagactg caaaaatcgg ttttctttgt
    12121 ttcaatgctt ccaagatcaa tgcctgttcg aattggtctc gttgaatatt tgtttccgtc
    12181 agttttggat gagggtcttc catgtaaagt tgcggactaa catcctgacc gcctgcaaga
    12241 atgagtttgt cgattttctc aattagttga ggcgctgaat ctggagacat gactggcaga
    12301 ataagaggta atccttgagc atctaaaact gcatccacaa atccctgcgg tgtatagctt
    12361 acttgatttc cttggaaagt atctgtcgct cgaatcagtt gattcccggc aatgccgatg
    12421 attggtttca tttttattcc ccctaaagta gactaattgt aacaaggatt taaaaaaatt
    12481 taaactatgt aagacttgcc cctatactta tctgttcttg ctgtgttact atctgtaaag
    12541 agaattttcg tgtgaaattc atgacgatag ttgaaaatat tgcttaattt gttataataa
    12601 aaagtaagca gaaataaaaa ggttgggcgt aagcttcaag gatctagttc ttcaaggggt
    12661 gagaaggaca ttttattgaa ggggcctgcc tatactaagt tatctttaga ggtacatact
    12721 ttcaatgaaa aaagaaagca tgtacctctt ttttaaatcg aaaggaagtg aagcaagtgt
    12781 tagaagcttt ggaacaaatc cgaaaagcag aagaagaaaa tgagaagcgt gctcgaaccc
    12841 tacggcagca gttgaagacc tatgcagaag aaaaagagca agaattggag ataatccaaa
    12901 cgaacagtcg gcaacagctg aaacagttgg tagaagaaaa agaacaattc gaaaagcaag
    12961 aattagaaaa agaaaaaatg aaattgaaag cagaagcaga caaggtgcaa gcaactttga
    13021 aagaccaata tcaaaagcat cagagtcaag caatcggtgc aatcattgaa agggtgaaag
    13081 aaacttatgg ccgtcactaa gatggaaaaa gtgaccctca tttcagataa aagaaatcag
    13141 gagaggattt tacaagcagt acaaggaatc caaaatgttg aaatccggga tctgtatcaa
    13201 aatagaacaa acaaccagtg ggtagaaaaa tattttccag ataccacgat tattgataaa
    13261 gaagcaaaat taagcgcttt aggcaatcgg ctgaaagaca tccaagaagc tatccaattc
    13321 atacaacacc acggcgatag ccgtcaaaag aaactccatt tgaaacgtca agaactttcc
    13381 ttacaagact tggaagcaaa ttattccgaa gaaaatttca tgaataaatt agaagaggtc
    13441 cttgatttaa aagaaaagtg ggaacagcta actgaaaaac gtgatcgttt agaagaagag
    13501 gaagaatggc ttcttcattg gcaacagctg gatattgctc caagtatgta tgcaagtaag
    13561 acgaccattt ttgagatggg gactgtgagt gcagcaaatt ttgaaagttt caaggaagaa
    13621 ctaagtaagc ttgatgacgt ttatatggaa gaaatagatt atacgccaaa acaagtttat
    13681 ctttcgtatg ttgctttaaa aaaatcagtt gctgctatag aagaaattgc tgggaaatac
    13741 gggttcaata aagaaacgta tccttatgaa caaaatccta aagaaaaatt gcaagaaacc
    13801 aaacagcaat tatccattgt tcacgagcag caaaaaaaga taacgacagc gttaggtcag
    13861 tgcagcggct atatccaaga atttgaatgg gtagaagaag tcacacttgc cgttgcagaa
    13921 cgggaaaaag taaaagaacg gtttatccat gccacttatc tgatcgtttt gcaaggttgg
    13981 gtagatgcag aagaaaaaca gaatttgatg catgtgttaa acgaaactgt atcagaagaa
    14041 gatatatacg ttagtttcga aacaccaaac tcagaagaga ttcagcagga agtccctact
    14101 aaattgaaaa accatccgct tatcgaacct tttgaattgc tgactgaaat gtacagtctg
    14161 ccaaaatatg aagaagtaga tccaacgcct tggatgacac cattttatct tgtcttcttc
    14221 gggatgatgg tcgcagatgt tggttacgga ttgctgatgc tcattgggac aatactagct
    14281 caaaaactat ttgttttacc ccgcgggatg aaacgtttcg taaaattctt tgagattctc
    14341 tcggtaccat cgatcatctg gggattgatc tacagttcat ttttcggaca ggcattacca
    14401 aaagaactat ttggtatccg cttgcctttc ccaatcctat ctacgacgga tgacgtcaat
    14461 acgattttga ttttatccgt gattttcggt ctgatacaga tcttggttgg attattcgtg
    14521 gcagcaaagg aaaatatcag gaggaaagat tacttgagtg ctgtcagtga tggttttgcc
    14581 tggcaaggga tcctgattgg gatcgttatt gcattgattg gtgtcatgtt gataaaaaat
    14641 acagcatttg tttatcttgg cgcggggcta tcgatcattg ctgctttatg tatcttaatc
    14701 gtgccgatta tccaatcgcc gtctaaagtc aagggtgctg ccaaaggagc gtataatctt
    14761 tacggattga ctggttatat cggtgattta gtcagttata cacgtttgat ggcactaggt
    14821 atttcaggcg gaagtattgg cgcggcattc aatatgctag ttgctttcat gccgccagcc
    14881 gctcgattca gtgtagggat ccttttgatc atcgctttac acgcactgaa tatgtttttg
    14941 acactgttaa gtgcctatgt ccatggtgca cgattgcagt atgtcgaatt ttttggaaaa
    15001 ttttatacag gaggcggacg tgcctttgac ccgctgaaaa cggcggaaaa atacgtcaac
    15061 atcaatcata agaaaaaaaa caataaataa ttggaggatt tttatacaat gatggattac
    15121 ttaattagtc aaaatggtgg aatggtattt gcagtattag cgatggcaac agcgacgatt
    15181 ttttcaggga ttggttcagc taaaggtgtt ggaatgacag gtgaggcagc agcagcgtta
    15241 acaacaagtc aacccgaaaa attcggacag gctttgattc tgcaattgct tccaggtacg
    15301 caagggttgt atggattcgt tatcgccttt ttgattttta tcaacttagg aaatgatatg
    15361 tcagttgtac aaggattgaa ctttctaggc gcttctctgc cgattgcttt tacaggtttg
    15421 ttttcaggta tcgcccaagg gaaagttgca gcagcaggta tccaaatttt agcgaaaaaa
    15481 cctgaacatg ctacaaaagg aatcattttt gctgcgatgg ttgaaacata tgcgatttta
    15541 ggtttcgtta tttccttcct attagtattg aatgcctaag cataaaggag gaaaagcaga
    15601 tgaacgccat tgaaaaaatc atctctcaga tgaatgaagc agcagagcaa gaaagagcgg
    15661 cgttagagca agaagagcga atgaagatcg atcaaaactt tgaacaaaaa cgtacgcaaa
    15721 tcgaaacaga acatcaaaaa caaaaagaaa aacaaatcga attacttgaa aaaaaatacc
    15781 gtcagcttag gaatcgccaa caagtagaag tacgacaaga aaatttgaat gcgaagcagg
    15841 aatttttacg acgattgttt gcagatgcaa tagctgaaat ggagaattgg gatgaatctg
    15901 aacaaatcca gtttatcaaa aattcattgt attcattacc tttgacaggc aaagtaacat
    15961 tcattgctgg agaaaaatca gcagcttatc tttctcagac attgctggat gaatggaaca
    16021 atgaacttcc ctttatgatg gttttaagtg atgaaactgt tgctgatcaa gcgggttttc
    16081 tgataaacga tcaaggagtt cagtataatt ttcttttcag cagccttgta caagatatcc
    16141 aaggaacgat gagttttgag attgccaata aattattcga ataaggaggg atcagtgtat
    16201 ggaataccac gaactaaatc ccttgattcg cggaagagag cttgaacttt tgtccaatga
    16261 gacatttgaa cggatgattc aaacaaattc tgtcgaagca cttggtgaaa tattgaaatc
    16321 cacgatttac agtccatata tttatgacgg atatgaatat gacttcgaac agaatctttt
    16381 aaaggagcag agccgtctat ttcgctggtt aaaggaatca gcaccggagc ctgaaatcgt
    16441 ttggatctat acgatgcgct atactttcca taatctgaag gttttgacaa aagccgagat
    16501 gacaaatcaa aatctggacc atttgtatat cgacgatgga ttttattcat tagaaacctt
    16561 gaaggatgcc attcatacgc aagtttcaaa tgaactccct gaaagcatta tggacagtat
    16621 acgagaagta cgtgattatt ttgaagagtc taccattctt cagggaattg acgtgattta
    16681 tgatcgacag tttttgacag agcagcgacg gcttgccgat aagctaggat atcaagaatt
    16741 gctgacagaa gtcattgctt ttattgactt gaccaatatc acaacgatgg ctcgaggaat
    16801 attgcagaaa agaacatatg ggttcatgac gacggtactt tccagctcgg gaagtatcga
    16861 aaaagaagta tttttgaact ttgtagataa agatatggaa gattacacga attttctatt
    16921 agagacagat tatgctgatt ttctgcgccc tgtcatccgt gagggaaatc ttgatctcgt
    16981 aaagcttgaa cagttgaaag atgattatct aagttcattg tatcaagaag cacaaaccca
    17041 ggctttcgga ccactgccac tactagcctt tttgaacgca aaagaggtag aaagcaaaaa
    17101 ccttcgttta ctggttatcg gcaaaagaag ccatttcgaa ccagaaacaa tcagaaagcg
    17161 ggtgagacaa gtctatgacg cataagatcg gtgtagttgg cgacaaagat tctgttttgc
    17221 cattcaaatt gtttggcttt gatgttcgtt atggtacaac caagcaaacc gttcgtcagg
    17281 caattgaaga aatggcaaag aatgagtatg gcgtgattta tgtcacagag caatgtgcag
    17341 cattgagccc agaaacgatc gaacgttaca aggaacaaat gatacctgct atcgtattga
    17401 ttcccaatca tcaaggatca ttagggatag ggctggaaga aattcaaaaa aacgtagaaa
    17461 aagcagtagg tcaaaatatc ttatagtatg aaaaggagat agaaattttg caaattggaa
    17521 aaatcataaa agtctccgga cctcttgtaa tggctgagaa catgtctgat gccagtatcc
    17581 aagacatgtg tctagtggga gatctaggag ttatcggaga aattatcgag atgcgtggcg
    17641 atgtggcgtc tattcaagta tatgaagaga cttctggaat tggtccaggc gaaccggttc
    17701 gctctactgg tgaagcttta tctgttgaat taggaccagg gatcatttca caaatgtttg
    17761 acggaatcca acggccatta gataccttta tggaaatcac acaaagtaat ttcttagggc
    17821 gcggagttca attgcccgca ttggaccatg agaaaaaatg gtggttcgaa ccaacagtag
    17881 aagccggaga aacggtgagt gctggagatg ttatcgggat cgtagaagaa acaaaagtta
    17941 taaaacataa aatcatggtg ccaaatggga taaaaggtac gatcaaaaca atcgaaaaag
    18001 gttcattcac tattgatgaa acagtatgtg tcattgaaac cgaaaatggt gacaaaaatt
    18061 tgacgatgct tcaaaaatgg ccagtaagac gtagtcggcc aatcaaagaa aaattaaacc
    18121 cggatgcacc catgatcact ggacagcgag tgattgatac attcttccca gtgacaaaag
    18181 gaggagcagc agctgtacct ggtccttttg gtgcagggaa aacagtcgtt cagcatcaaa
    18241 ttgcaaaatg ggccgatgtg gacatggttg tctatgtggg atgtggcgag cgtggaaatg
    18301 agatgacgga tgttttaaat gaattccctg aattgattga ccctaataca ggagaatcgt
    18361 tgatggaacg tacggtattg attgctaata catcaaacat gccagtagca gctcgagaag
    18421 cttctatcta tacgggaatc acaattgcgg aatatttccg tgacatgggt tatgacgttg
    18481 ctatcatggc agactccact tcacgatggg cagaagcact tcgtgagatg agcggacgtt
    18541 tggaagaaat gcctggtgat gaaggttatc cagcttatct tggttcacgg cttgcggaat
    18601 actatgaacg atctggaaga gtcattgcat taggatcaga aggtcgcgaa ggaagtataa
    18661 ccgcaatcag tgctgtatcg ccgtctggag gggatatttc ggaaccagtc acacaaaata
    18721 cattgcgggt cgtaaaagta ttctggggtc ttgattccag tttagcccaa aaaagacatt
    18781 ttccttcgat cgattggata caaagctatt ctctttattc aacagaagta gggaagtata
    18841 tggatcatat ccttcaagct gactgggcat ccatggtcac cgagggcatg cgtattttgc
    18901 aggaagaaca gcagctgaat gaaatcgtgc gtttggtagg gattgattcc ctttcagata
    18961 atgaccgtct gacattagaa gtggcaaaat ccattcgtga agattatttg cagcaaaatg
    19021 cttttgatga tgtcgataca ttcacttcaa gagagaaaca attcaaaatg ttgaagatga
    19081 tcttgacctt tggtgaagaa ggacgccgtg cgttgtcgtt aggtgcttat ttaagtgaga
    19141 tcatggacgg taccgtatcg attcgtgaaa ggatcggacg aagcaaatat atcccggaaa
    19201 cagaattaga aaaactagat caaattattg gtgaaatcaa gcaaacgatg caaacgatcg
    19261 tttcagaagg aggcatgagc aatgattaaa gaatatcgta cgatcggtga agtcgtcggt
    19321 ccattgatgg cggttgaaaa agttgctggt gtcaaatatg aagaattaat cgaagtccgt
    19381 atgcaaaatg gcgagattcg tcgtggacaa gtattggaag tccaagaaga taaagcaatg
    19441 gtccaaatat tcgaaggaac aagtggtatc aatttacgtg actcttctgt acgattttta
    19501 ggccatcctt tagaactagg tgtatcggaa gacatgatcg gtcgagtatt cgatggtttg
    19561 ggacgaccga aagataacgg accagagatc ctgcctgaaa agcatttgga tattaacgga
    19621 gaagtaatca atccagtagc tcgggattat ccagatgagt ttatccaaac aggtatctct
    19681 gcaatcgatc atttgaatac attagttcgt gggcaaaaat taccagtatt ttctggctct
    19741 ggtcttcctc ataaagaatt agctgctcag atcgcgcgac aagcaacggt actcgattca
    19801 tcagatgatt tcgccgtagt ctttgctgct attggaatca cttttgaaga agcagaattt
    19861 ttcatggaag attttcgtca aacgggcgca atcgaccgtt ctgtcatgtt tatgaattta
    19921 gcaaacgacc ctgcaatcga gcgtatcgct actcctcgta tggctttgac tgctgcagaa
    19981 tatcttgctt atgaaaaggg aatgcatgtt ttggttatca tgacagacat gaccaactat
    20041 gcagaggcat tgcgagagat ttctgcagca cggcgagaag ttccaggaag aagaggatat
    20101 cctgggtatc tttacacgaa cttagctact ttattcgaac gagcaggaag aatccgcgga
    20161 ctaaaaggat cggttactca aattccgatt ctaacaatgc cagaagacga taaaactcat
    20221 ccaatccctg acttgactgg ttatattaca gaaggacaga tcatcttgac tagagagctt
    20281 tacaagagtg ggattcagcc accaattgat gtactcccgt ctctttcgcg gttaaaagat
    20341 aagggaactg gacccggaaa gacaagagaa gaccatgcag ctacgatgaa tcagcttttt
    20401 gcagcctatg cacaaggaaa acaggcaaaa gaattggccg tagttttagg tgaatctgct
    20461 ttatctgatg tagacaagat ctatgcgaag tttgctgagc ggtttgaaaa agaatatgtc
    20521 aatcaaggat tctacacgaa ccgttcgatt actgaaacac ttgatctagg gtgggaactg
    20581 ctatcgatgt tgccaagaac cgaattaaaa cgaatcaaag acgacatgct ggacaaatat
    20641 ctgccagaag ggaagtgagt tctgatggca cgattgaatg tgaatccgac ccgcatggaa
    20701 ttgactcgat tgaaaaaaca gttgacaact gccacaagag ggcataaatt attaaaagat
    20761 aagcaagatg aactgatgcg tcaattcatc ctgttgattc gaaagaacaa ccagctgcgt
    20821 caagaaatgg aagagtctat gacagaagca atgtctgatt ttgtattggc aaatgcttcg
    20881 atcacggagc ctttcatcga agaattattt gttttgccgg cggaaaacgt tgatttgtct
    20941 atcgtagaca aaaatatcat gagtgtaaaa gtacctgtga tgaattttga atatgatgaa
    21001 cagctgacag acacaccttt ggaatacggg tatttgaatt ccagtgccga actggatcgg
    21061 tcaatcgatc ggtttactac cctgcttcca aaacttttgg aactgacaga aatcgaaaaa
    21121 acttgtcaac tgatggcgag tgaaatcgaa aagacacgtc ggcgagtcaa tgcattagaa
    21181 tatatgacga tccctcaatt agaagaaacg atctattata ttcgtatgaa gctggaagaa
    21241 aacgagcgcg cagaagtaac aaggttgatc aaagtaaaaa atatgggtac cactgactaa
    21301 gtggtatcct tttttaaaag aggaaaacga tgattaaaca aagcaaacaa ataaagaaac
    21361 gcttgccggc tgttcagctt ctcgcattag gattttttct cctgatttta gttggcggcg
    21421 gcctacttac cctaccattt tttagtaata gtgggcatgg gacaaatttt atcgatgcgc
    21481 ttttcactgc aacttctgct gtctgtgtga cgggattgac aacattgaat acagcagagc
    21541 attggaacag tgctggtcaa tttttgatca tgcttttgat tgagatcggc ggtcttggtt
    21601 ttatgatggt tccgattcta tgctttgcat tgataaagaa gaaagtcagt ctcagcacac
    21661 gtattatatt aaaagaagcg ctgaatctag aagaagtatc aggtgtgatg aagctcatgc
    21721 tttacatcct gaaattggcc tttgtgatcc aactgattgg tacggttgct ttatcttttg
    21781 tattcgtgcc tgaatttggc tggttgaaag gtatctggta cagtttgttc catgcaatct
    21841 ctagtttttg taatgcaggg tttgatttgt taggagacag tttagccaat caccagcaaa
    21901 atgtttattt gatcatggtg gtatctgctt tgatcatttc tggtgggtta ggcttcatcg
    21961 tatggcgtga tttactggaa tatcatcaga aaaagaagat aacgattcat tcaaaaatcg
    22021 cattaaccgt tacaggtacc cttttattag gcggattcgt tgtgttcttt ttcacagagc
    22081 gcaatgggga aacgctagtg gaagggaata tgtttcaacg attggcaaac acctttttta
    22141 tgagtgtgac acctagaacg gctggatatt attcagttga ttacctccat atgagccatg
    22201 caggtctgat tttaacgatg tttttgatgt acatcggtgg aacatctggg tcgacggccg
    22261 gcggattgaa aacaactacg ttaggtgtgt tgctgatcca gatgcgttcg atgcttaaag
    22321 gacgtacgag agcggaagca tttggccgta ccatccgaca aggcgcagtg ttgcgggcac
    22381 ttactttatt ttttgttacg ttgacgttgt gtgtagtcgc catcatgatt ttatcggtga
    22441 ctgaaacgat acctaaaact tctggcatcg aatatattgc ttttgaggtc ttctctgcgt
    22501 ttggtacagt aggtcttacg atgggattga cgccagactt gactgcaata gggaaattga
    22561 tcattatagc gcttatgtac attggacgag ttggtgtatt gactgtggtc ttctctctga
    22621 tgacaaaagc acaaagaaca caatcaaaat acaaatatcc agaagaaagt gtcatgattg
    22681 gataatttca tcttctgaca gaaacctagt gatttagtaa aataagaaaa taaaataatt
    22741 tttcttgctt cttggatact ttaagtgcaa aatgttcgtt tattttttga ggcactatcc
    22801 gttgttgaac ctcgggtagt gcctctttct tattgaaagg aatcaataag atttgattcc
    22861 tttatttatc aataggttat aattcatatt tttttatttt ttcttttacg atacttagtc
    22921 cagaagctaa aatttcaagg ttattagagg atagggaaat tcgaacgaat gcatctggtt
    22981 gcgcacgtgt agtaaagcgt ctggaatgat aaagtcggat accgttagca agaaagtcat
    23041 tctcaattat cttaggatca atctcaggat caactggaag aattcgatag taagggacac
    23101 gattcgttgg tttttctact ttgaaaagag aatcaaaaag ctgattggct cttttcgtaa
    23161 gacttaattt agtttctaag atcctatttg cttcatttga atggaggatt tgagtgatga
    23221 cttccacatc taatccagaa gttttcacat ttatattaaa aattgcttgt tctatcttgt
    23281 tttggacatg tactggaact acaagataag caactcgcaa tccagaacaa ataaattttg
    23341 tcattccagc taagtagatt gtttggttag gtaataattt ttggaaagga ggaatgatcg
    23401 tatgttgata gtaagttgtt aaaaatgaat ggatatcatc ttcaataacc catagacctt
    23461 ctttttcaat cacttcggct aattgctctc gtcttttgtg agagatttga aaccctatag
    23521 gattattaca agaaggcata agaaagacac catgaatctt cttttttcta cattcctgaa
    23581 tcaatatatc aggtcgcatt ccttgctcgt caaaatcaat tggaacgatc tctaaatgat
    23641 ataactgagc caattcaatg aaattagcgt atgtatatcg gtctgttgct attctatcac
    23701 ctggagagaa aagtgcagaa agtgtgatag ccaatccatt ttgaacaccg gaaacaatag
    23761 atagagattc taaggacgga atgactcctt gagtttttaa ccaatttgct gcagtagtca
    23821 attgaaaatt tgtgcctttg ggatcttggt aattaagcaa accaacaaga tttgggttat
    23881 tactagttga ttgaataaac gggacaacga atttattaca ttcttcaaaa gaactaacca
    23941 gaccaaaatc aatgatatgt tcggttactt cattggtaga gatggtgatg gactcaaaga
    24001 cattgggaga aataaatgtg ccacttccaa tattcgtata cagcaaacct ttctcaattc
    24061 catatttata agccttgcca acggtagtaa aatttaaatc taaataatca gctagctctc
    24121 tttgggaggg taactgagtg tttttctgta atttattctt taaaatatct tgttctaatt
    24181 gttgaatcaa cgatcgataa taaggacgct tcaatttttg tttgtctggt cgccaagtta
    24241 acgggtaatt gtcataagaa ttaataggca ttttctacac tccttaaaat tgtataccat
    24301 acaattttac cattgattgt attgatttta gaagttaatc ttttgtgagg agggaattgt
    24361 ctatgaaaat tattaatttt gaggaagtag tagcttcttt tagttttgaa gaagcgattc
    24421 aagtaatgaa aaaatgtttt tttgattatg aaagtggtaa aatcagccaa aatcctcgat
    24481 tggtggaaat tttacctgac ggaaaagaca aaaatatgtt tgcgatgatg cctgcttatt
    24541 tgggggagaa cagatatttt ggaacaaaag tcattaccgc ttttccggat aatcatcagt
    24601 atcatttacc ttctcattta ggagagattt tcttgtttga tgctcaaaac ggactaccca
    24661 aagcattgat aaacgcaaat gcagtcacct ggattcgaac agctgctgta tctgcgctgg
    24721 caacagatta cttagcaaat ccgcatgcag aaacacttgc tttaattgga gctggtcaac
    24781 aagcattgtc gcatttgact gcgatttcta ccattcgtcc catcactaca gtgtttgttt
    24841 acgatatttt agaagaaaga cgaaatactt ttatcgctaa agctaaaaaa cagtatccta
    24901 aaataacgtt tataaatgca atatcagtag aagatgcagt acgtccagcg gaaatcattt
    24961 gtacgttgac ctctagtaaa gaggcatttc ttcaaaaaga gtgggtaagg gaagacgcac
    25021 atatcaatgc tgtgggcaca tttactcctg atacaagaga gataagtaca tcgttgatgg
    25081 aaacttctca aatctatgta gatgattact cagctacctt aacagaaagc ggggacctgc
    25141 ttatagccat ggcagaagga gatttagagg aatcttgtat tgttggtaca ttaggcgaat
    25201 tagtttctga gaaaaaaaca ttttataaag gagaatcgag gccgacgatt ttcgacgcag
    25261 ttggtctagc tattgaagat ctttgttgtg cagaatatat ttataacaaa ttagagaaag
    25321 gggctttaaa atgaacttag attattacgt agttgatgca tttgcagatg aggtgttcaa
    25381 aggaaatcca gcagcggttt atgtgctaga agaatggctg ccagaaggta cgatgcaaaa
    25441 aattgccatt gaaaacaatt tatctgaaac agcatttaca gtgaagaaaa accaagaatt
    25501 tgagcttaga tggtttacgc cagatcgaga aatcgatctt tgtggtcacg cgacattagc
    25561 aacagcattt gttttattta actattacaa aatacctgat gaaacgatta aattttctac
    25621 tcaaagcggt aatctatttg tgacaagaat taaagattat tattacatgg attttcctag
    25681 tattatgccg aaaaaagtgc cgatattgac tgaatatgaa gaagcaattg gggctaagat
    25741 taaagaagcc tacttagcac gtgatttatt tttcgtatta gaagatgaaa aaacggtggc
    25801 taaattgcag ccagatttta cagctatcaa aaattttgaa ttaggaatag agtcatcgtg
    25861 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    25921 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    25981 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    26041 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    26101 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcagcctta
    26161 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    26221 ctttttttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    26281 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    26341 cgtagtgcca ggtaatcctt ttaagtcagt aatctcaaga cctgcagctt tatgttgctc
    26401 gtgtaatttt tctaaatcat cagctgcaat agcgatatga ccatagccgt ctcctagatc
    26461 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatctc caggaagtgt
    26521 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    26581 aaatgccttt tgataaaatt cgattgatgc t
//
LOCUS       DACCQI010000031        26427 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCQI010000031 DACCQI010000000
VERSION     DACCQI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.031"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(130..825)
                     /locus_tag="IVO76_001006"
     CDS             complement(130..825)
                     /locus_tag="IVO76_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7428983.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IVO76_001007"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IVO76_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7428984.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IVO76_001008"
     CDS             complement(2024..2215)
                     /locus_tag="IVO76_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7428985.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IVO76_001009"
     CDS             complement(2236..3126)
                     /locus_tag="IVO76_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7428986.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IVO76_001010"
     CDS             complement(3113..3880)
                     /locus_tag="IVO76_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7428987.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IVO76_001011"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IVO76_001011"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7428988.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IVO76_001012"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IVO76_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7428989.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IVO76_001013"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IVO76_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7428990.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IVO76_001014"
     CDS             8496..8834
                     /locus_tag="IVO76_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7428991.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IVO76_001015"
     CDS             complement(9002..9760)
                     /locus_tag="IVO76_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7428992.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IVO76_001016"
     CDS             complement(9778..10596)
                     /locus_tag="IVO76_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7428993.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IVO76_001017"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IVO76_001017"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7428994.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IVO76_001018"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IVO76_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7428995.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11724..13058
                     /gene="dnaA"
                     /locus_tag="IVO76_001019"
     CDS             11724..13058
                     /gene="dnaA"
                     /locus_tag="IVO76_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7428996.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13256..14386
                     /gene="dnaN"
                     /locus_tag="IVO76_001020"
     CDS             13256..14386
                     /gene="dnaN"
                     /locus_tag="IVO76_001020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7428997.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14615..14857
                     /gene="yaaA"
                     /locus_tag="IVO76_001021"
     CDS             14615..14857
                     /gene="yaaA"
                     /locus_tag="IVO76_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7428998.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14844..15968
                     /gene="recF"
                     /locus_tag="IVO76_001022"
     CDS             14844..15968
                     /gene="recF"
                     /locus_tag="IVO76_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7428999.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15965..17911
                     /gene="gyrB"
                     /locus_tag="IVO76_001023"
     CDS             15965..17911
                     /gene="gyrB"
                     /locus_tag="IVO76_001023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7429000.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17931..20402
                     /gene="gyrA"
                     /locus_tag="IVO76_001024"
     CDS             17931..20402
                     /gene="gyrA"
                     /locus_tag="IVO76_001024"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7429001.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20638..20937
                     /locus_tag="IVO76_001025"
     CDS             20638..20937
                     /locus_tag="IVO76_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7429002.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20987..21499
                     /gene="ssb"
                     /locus_tag="IVO76_001026"
     CDS             20987..21499
                     /gene="ssb"
                     /locus_tag="IVO76_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7429003.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21525..21761
                     /gene="rpsR"
                     /locus_tag="IVO76_001027"
     CDS             21525..21761
                     /gene="rpsR"
                     /locus_tag="IVO76_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7429004.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21904..23877
                     /locus_tag="IVO76_001028"
     CDS             21904..23877
                     /locus_tag="IVO76_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP7429005.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23883..24335
                     /gene="rplI"
                     /locus_tag="IVO76_001029"
     CDS             23883..24335
                     /gene="rplI"
                     /locus_tag="IVO76_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7429006.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24609..25976
                     /gene="dnaB"
                     /locus_tag="IVO76_001030"
     CDS             24609..25976
                     /gene="dnaB"
                     /locus_tag="IVO76_001030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7429007.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         8017 a         5215 c         5235 g         7960 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaaca
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggaatccta tgtggaaaaa gttatgcaca gggattatcc acagataata aaaggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctgtat tttttatcca cagacgcaag aggaaatcta
    11521 ttggttttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt tttgacaact
    11581 gtgtttttct cttttttctt tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt
    11641 tcttcggttt ctgtggaaaa ctattgaaaa aaatgctaaa ttagtactag ttattcactt
    11701 tttaatgaag gaggaggcct tatatggtat ccctcgatgc tttatggaat gaattaaaag
    11761 caacatacca aaaggattta tcgcctgcta gctataatac atggatcgaa acagcgaacc
    11821 ctcgaaccct tgatcaaaac cagctcgttg ttgaggttcc tagcaagatt cataaagaat
    11881 attgggaaaa aaacctggcg acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg
    11941 agattatccc tcgctttatt actggcgaag aagcagagca agaagaagtt atagaagaaa
    12001 aaaatccaaa agttgtggca cctagtccgc taaaaaaagc catgctgaac cctaaatata
    12061 cctttgatac gtttgtcatt ggtaaaggaa accagatggc tcatgcagca gcgctcgttg
    12121 tagcagaaga tccaggttct atttataacc cgctcttctt ttatggaggc gtgggacttg
    12181 gtaagactca cttgatgcat gcaattggtc atcaaatgct gcaaagtcag cctaatgcca
    12241 aagtaaaata tgtaagcagt gaaacttttg ctaatgattt catcaattcg atccaaaaca
    12301 aaacagcaga agagttccgt caagaatatc gaaatgttga tttactgcta gtggatgaca
    12361 ttcaattttt tgcagaaaaa gaagctacgc aagaagagtt tttccatact ttcaatgcct
    12421 tgtacaatga agggaaacaa atcgtactaa ccagtgatcg gttgccaaac gagattccga
    12481 aactgcaaga acgccttgtt tctcgctttg catgggggct gtctgttgat attacaccgc
    12541 cggatcttga aacaagaaca gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga
    12601 tcccagatga cacactaagc tacatcgcag gacaaattga ttctaacatt cgtgaattag
    12661 aaggtgcgct tgttcgagtg caagcttttg ctgcgatgaa tagtgaagat atttcgacta
    12721 gtctagctgc agacgcactg aaaacgttaa aatcaggaaa aggacatcct caattgtcaa
    12781 tcctgcagat ccaagaagaa gtggcaaagt attatcacat ccaattgaaa gatctcaaag
    12841 gaaagaaacg cgtcaaatct attgttgttc ctagacaaat cgctatgtat ttggcacgtg
    12901 agctcacaga taattcttta ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa
    12961 cggttatcca tgctcatgaa aaaatccagc agctgatgga ttctagtgtc agtatgcaaa
    13021 atgaagtatc agaaatcaaa aactttttac taaattaatt tgtcgattct atcaactgaa
    13081 aatagcatgt ggataaaaga aaaaaacgtt caaaagttat acacactttt tccacagctg
    13141 ttttttcgtt tctctcttac gttttttgag ttttccacag aatcaacatg ccctattact
    13201 tttattattc tttttaacta ataataaata aacaaacatt caaaggagat acatcatgaa
    13261 agttacttta aaccgagcta gctttatgca ggaattgcaa actgttcaac gagctatttc
    13321 aagcaaaacc acgatcccta ttttgacagg tgtaaaaatc acactgacac aagaaggttt
    13381 gactttgacg gggagcaacg ctgatatatc aattgaaact tttttgtctg ttgaaaacga
    13441 aaaagcaaat atgcaaatcg aatctactgg ttccattgtt ttacaagcac gtttctttag
    13501 cgaaatcatt cggagacttc ctgaagaaac atttacttta gaagttttag aaaataaaca
    13561 agtagcgatc acttctggaa aagcgaattt tatcgtaaat ggattagatg cagataacta
    13621 tcctcatctt cctgttgtcg aaagccataa ccagatgaaa ttacctgtac acgtattgac
    13681 taaactaatc aacgaaacag tttttgctgt ctcacaacat gagagtcgtc caatcttgac
    13741 aggtgtgcat ttcattttat ctgataattc tttattagct gtagctactg attctcaccg
    13801 tctaagtcaa cgcgtgattc cagtagaaca agcggctgat cattttgata ttgttattcc
    13861 tggaaaaagt ttgatcgaat tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat
    13921 cagcattatg gaaaaccaag tgttgttcaa aacagaaacg atgtatttct attcccgttt
    13981 gctagaagga aactatccag ataccaatcg tttgattcct tcaagtttta atacagaggt
    14041 tgaattttct gttcctagct ttttagcggc gatcgaacgg gcttctttac tttctcatga
    14101 aggccgtaac aacatcgttc gtttatctat tcgtccagat gcggttgttt tatacggaaa
    14161 ttcgccagaa atcgggaaag tagaagaaag cttgagttat acagcaagca gtggcgatcc
    14221 attagatatt tctttcaatc cggattatat gaaagcagcg ttgcgtgcat ttggagatat
    14281 gagcatcaaa gtgaaattta tctctgctat tcgtccattt acattggaac caacggaaga
    14341 tggcgttcaa tttattcagc tgattactcc tgtacgtaca aattaacaat gattttcaat
    14401 gttcttgaaa atttaattga aaagaaatag aaaaaataaa attttaaaca gaaggcccta
    14461 aaatctcttt taaggctttc tgtttatttt tgtttaaatc acctttttta gattaaatcg
    14521 tcttatatgg aaaaatatat ggataaattt gttttattgc gaaaaaaaag gtataataga
    14581 gtatactttt aataatgaaa atgagggatg ggcattgaaa caacaagtca ttctaaatac
    14641 ggaattcatg acgttgggcc aaatgttgaa agaagtcaca gttatcggta gtggcggaca
    14701 agcaaaatgg tatttggcag aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg
    14761 tggacgcaaa ctttatcccg gcatgatggt tgaagttcca gaagtaggaa ctttttttat
    14821 ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct gtatttaaaa aattatcgaa
    14881 attacgaaga gctgaacatc gaattttcca agaacctcgt catcttttta ggagaaaatg
    14941 cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt agctatgacg cgaagtcatc
    15001 ggacaagtag tgagcaggaa ttgattggct ggactgacga tcaggcgatg attcaaggag
    15061 agatcacaaa aggttcatcc actcttccat tagaaatctt attatctaaa aaaggccgaa
    15121 aaaccaagat caaccatatc gaacagaaaa agttaagtac ttacgtggga caaatgaatg
    15181 tcattttatt tgcaccagaa gatctttctt tagtaaaagg cagcccgcag accaggcgga
    15241 agtttttaga tatggaatta ggccagatca atccaatcta tctttatgat cttgtccaat
    15301 accaagcaat tttgaaacaa agaaaccagt acttgaagca attgaatgaa aaaaaacaaa
    15361 ctgatttgct ttatttggat attttgtccg aacaactagc agcgtttggc agcaaagtat
    15421 tgaaagcaag agcacagttc gtcaagcgat tggagttttg ggctaattct ttgcatcagc
    15481 aaatcaccca tcaaaaagag cagctggaaa tcgagtatct caccgcagtc gattctttag
    15541 aaacacatac gcaagagcaa atccaagaac aatttctcgc acttttgaac caaaataaaa
    15601 agaaagacct ttttcgggga acaacgacag ttggtcctca tcgtgacgat ctttcctttt
    15661 ttattaatca gaaaaatgtg cagacttatg gatcgcaagg acagcaaaga acaactgcac
    15721 taagtgtcaa attagcagaa atcgatttga ttaaagaaga gacaggggaa tatccaatcc
    15781 ttttgttaga tgatgtgatg agcgagctgg atgataatcg tcaactccat ctgctagaaa
    15841 caatcgaagg aaaagttcaa acgttcttaa caacaacgac acttgaccat gtaaaaaata
    15901 aaatgactgt tgaacctgag attttttatg ttcaacaagg gaaactaaaa ggagaagaaa
    15961 ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa gagtatgatg ccagtcagat
    16021 tcaagtactt gaaggacttg aagccgttcg taaacgacct ggcatgtata tcggttcgac
    16081 tagttctgag ggtctgcatc accttgtttg ggaaattgtc gataattcga tcgatgaagt
    16141 attagcaggt tttgcaacga aaatccatgt gattattgaa aaagacaaca gtatcaccgt
    16201 tattgacgac ggtcggggga tacctgtaga tatccaggca aaaactggac gtcctgcagt
    16261 tgaaacggtc tttactgttt tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt
    16321 ttctggtgga cttcacggag taggatcttc tgttgttaac gcgctttcta cacagcttga
    16381 tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt
    16441 ggacgactta aagattatcg aacaaacgga tcgccatgga acaacggttc actttacgcc
    16501 agaccctgaa atttttacag agacaactga gtttgatttt tcaaaattgg ctacccggat
    16561 ccgtgaactt gctttcttga accgcgggat gcgtatatcg attgaagaca aacgagaaga
    16621 agaaccagtc atcaatgaat accattatga cggcgggatt aaaagttatg tcgaatactt
    16681 gaatgcaaac aaaaccgtga tttttccaga acctgtatat ttggaaggtg aacagcaaga
    16741 tatcgcagtt gaagtctcta tgcaatatac agacggctac cattccaata tcatgagttt
    16801 tgccaacaat atccatacgt atgaaggcgg aacacatgaa tcaggattca agactgcttt
    16861 aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa
    16921 cttgaccggt gaagacgtgc gagaaggatt gacagctgtt atttctatca agcatcctga
    16981 ccctcaattc gaaggacaaa caaaaacaaa attgggcaat tccgaagtac ggacagtcac
    17041 tgatcggtta tttagcgaac atttcatgaa attcttgctg gaaaacccaa gtgtgggccg
    17101 tcaaattgtt gaaaaaggat tactagcttc cagagctcgt ctagcagcga aacgtgcgcg
    17161 tgaagtaacg agacgtaaag gtgctttaga gattagtaat ctgccaggaa aattagcgga
    17221 ctgctctagc aacgatcctg aaaaatgcga attgttcatc gtcgaaggag attcggccgg
    17281 cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa
    17341 aatcttgaat gttgaaaaag caagcatgga caagatcttg gcaaatgaag agatccggtc
    17401 tttatttact gctatgggta cagggtttgg agaagatttt gatgtatcga aagctcgcta
    17461 ccacaaatta gttatcatga ctgatgccga tgtcgacggc gcgcatatcc gtacattgct
    17521 gctgactctt ttttatcgtt acatgcgtcc tattgttgaa gctggatacg tctatattgc
    17581 tcagccacct ttatacgggg taaaacaagg gaagaatatt acgtatgtac aaccaggaaa
    17641 aaatgcagaa gaagagttga aacaagtcgt tgcttcactt cctgcctcac caaaaccaag
    17701 cgttcagcgt tataaaggac ttggagaaat ggatgatcat caactatggg aaacaactat
    17761 ggacccaagc aatcggatga tggcccgcgt cagtgtggac gatgctattg cagcagatca
    17821 gatatttgaa atgttgatgg gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa
    17881 tgcacactat gtgaagaact tagatattta atcatgaagg aggaaaaaga atgagtgaag
    17941 aaatcagaga aaacatccat gatgtcaatc taaccagcga aatgaaagac tccttcatcg
    18001 attatgcgat gagtgttatt gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac
    18061 ccgttcatcg ccgtatttta tacggcatga acgaattagg tgtaacaccg gataaagccc
    18121 ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa ataccatcct catggagact
    18181 atgcgatcta tgagtcaatg gtacggatgg cacagccatt cagctaccgt tatatgctag
    18241 tggatggtca tggaaacttt ggttctgttg acggtgacgg tgccgccgct atgcgttata
    18301 ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg tgatatcaat aagaacactg
    18361 ttgatttcca aagcaactat gatgatacag aaaaagaacc agttgtacta cctgctcgct
    18421 tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc
    18481 cacctcataa cttgtctgag gtagtagcgg ctatcgattt gctgatggat aatcctgatg
    18541 tgacgaccaa tgaattgatg gaagtattac ctggaccaga ttttccaaca ggtggtttgg
    18601 ttatgggaaa atcaggaatc cgaagagcgt atgaaacagg taagggttct attaccgtac
    18661 gtgcaaaagt tgagattact gagatgccaa atggtaaaga acggatcatc gtgacagaat
    18721 tgccttatat ggtcaataaa gcaaaattga tcgaacggat ttctgaactg cacagagaca
    18781 agcgtatcga agggatcaca gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg
    18841 tcattgacat ccgtcgtgac gccagcgcat ctgttatctt gaataactta tataaattga
    18901 catccttgca aaattcattt ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag
    18961 ttttgagctt gaaacaaatt cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa
    19021 gacggacagt ctttgaaaaa gaaaaagcag aagcaagggc acacatttta gaaggtttgc
    19081 gtattgcttt ggatcatatc gatgaaatca ttgcgattat ccgcgggtct aaatcagatg
    19141 acgaagcaaa agcgacgatg atcgaacggt tcgatttatc cgatcgccag tctcaagcta
    19201 ttttagatat gcgtttacgc cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat
    19261 accaagaact cttaaaactg attgaagatt taactgacat tctggcaaga ccagaacgtg
    19321 ttacagaaat catcaagaat gaattagcag acttgaatca acgatttggt gataaacgac
    19381 gcacagaatt actagtcggt gaggtcttaa gtctggaaga tgaagattta atcgaagaag
    19441 aagaaatcgt tattacattg acaaacaatg gttacatcaa acgtttggca aataatgaat
    19501 tcagagctca acgaaggggt ggacgtggtg tccaaggaat gggtatccac aatgacgact
    19561 tcgtcaaaaa tctggtttct tgttctaccc atgatacatt gttattcttt acgaataacg
    19621 gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa
    19681 taccaattat caatttacta ggtatcgatt cgagtgaaag catccaggca atcattgcgg
    19741 tgacgggaga agcagaagaa ggccactatc tcttctttac gacgcgtcaa ggtacagtaa
    19801 aacgtacttc tgtcactgct tttgctaaca tccgaagtaa tggtctgatc gcaattggtt
    19861 tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga tggtcaatct aatatcatta
    19921 ttggaacaca taacggctac tctgtcactt tcaaagaaga agcagtccgc gatatgggac
    19981 gtacagcttc cggggttaga ggaatacgcc tgcgtgagga agattatgtc gttggggcag
    20041 ccttaatgaa ggaagatcaa gaagtattag tgatcacaga aaaaggttac ggaaaacgga
    20101 caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca
    20161 tcactgaaaa aaatggtcca ctagctggat tgaccactgt ttctggagat gaagacatca
    20221 tgctgattac agataaaggt gtcattatcc gtttcaacgt atcgactgtt tctcaaactg
    20281 gacgttcaac attaggcgta cgtttgatga agatggaagc tgatacgaaa gttgtcacga
    20341 tggcagcagt cgaaccagaa gctgcagaac aaacagttga agaacaagga tcagaagaat
    20401 agccatcaag actaattgtg taagctagca agaatgaccg attaaaaaat ctggttattc
    20461 ctgctggctt ttttgattag ttgaatttct cttaaaatat tcgaaactgt tcattctgta
    20521 ttgcttttga ccaaaataga gtgtataatc agagcttgtg agtagtctaa ttaattagat
    20581 aactctcctt gctcttagga acctaagagc cgaaagtcca cagggaggtg aatatcaatg
    20641 gaaaacacga aatatgaaat catgtacatt attcgtccaa acattgatga agaagcaaaa
    20701 actgctttaa tcgaacgttt cgatgcaatc ttgaaagata acggagcaga agttatcgaa
    20761 tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga acggttaccg tgaaggtatc
    20821 tatcacatcg ttaaagtatc ttctccatca tcagcaaatg ctgtcaatga atttgaccgt
    20881 cttgctaaaa tcaacgacga tatcattcgt cacatgattg ttaaagaaga agcttaattg
    20941 ttgtttcatg tgaaacaata gaaaacgatg agaggagatg actattttga ttaataatgt
    21001 tgtattagtt ggacgtctga cgaaagatcc agatttgcgt tacacagcaa gtggtacggc
    21061 agtcgcaaca tttactttgg ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga
    21121 ggctgatttt atcaactgtg tgatttggcg caaatcagca gaaactttag caaactatgc
    21181 tcgtaaagga acacttttag gtgttactgg acgtatccaa actcgttctt atgataatca
    21241 gcaaggacaa cgtgtatacg tgacagaagt agtagctgac aacttccaat tgctggaaag
    21301 tcgttctgca tctgaaaatc gccaacaaag cggcggattc caaagttctg gtcaatcagc
    21361 tggcggattt ggcggaaaca ataactctaa tcaaacatct caatcatcca acggtatgcc
    21421 tgatttcgac cgtgatactt ctgacccatt cggctcaagt tcaacgatcg acatttcaga
    21481 cgatgattta ccattctaaa cgaatttgat aggagggaaa tagaatggca caacaaagaa
    21541 gaggcggacg taaacgtcgt aaagtagact acattgctgc taaccatatt gaatatatcg
    21601 attataaaga tatcgaatta ctaaaaagat ttatttcaga acgcggtaaa atcttgccac
    21661 gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg
    21721 ctcgtatcat gggcttgcta cctttcgtta gcgaagacta ataaatccta ggaattgaat
    21781 gactgatcct tttgatcagc tattcaattc ctttttttct gtaaaaataa cctctattcg
    21841 tttagtcgct cgaatgacga aagttatggt aaaatcaaat tagtcatata tgggaggaaa
    21901 attatggaaa ataaaaacaa tcatttatcc ccgaaacctc tcttccttat cctgtttata
    21961 ttaatcgaag tattggtagt cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt
    22021 ctattgatga atttatgggg tgttcgctat tggttgaaaa tgaaacgttg gcttatttta
    22081 aataaacgtg aaaaaatcca gcaggcttcg actgtagctg aaaccaatct agcttatgtc
    22141 tcagaagtca tgcctgtagg agtgattgct tatcagccat caagccaaaa aatcgaatgg
    22201 ctcaatccat tagcattgaa aatcaaagat caaagtggat taacagagga agaattgcta
    22261 gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag tctatctagc agataaaaca
    22321 tttcagtttg atatggatac agtaaagggt gttgtttatt ttaatgatat tacccaaaac
    22381 cagttgttaa aactccatca gacgaacagt cagccggtga ttggtatttt atctattgat
    22441 aactatgcag atgccattga taaattggat gacaaagaga tttcttattt gaactctttc
    22501 gtcactacac tagtttctga ctggatgaat gaataccatg tctttttcaa aagaatcaac
    22561 gcagaacggt ttttcttcgt agcacagatg atcgatctga aaaagatgat ggataaaaaa
    22621 tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt
    22681 agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat
    22741 aatttagatg ttgctttagt ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa
    22801 ggagcaaaac ctgtttatta tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt
    22861 cgttctcgag cgatgagtac cgctttgcgt cggatttttg atgaaacagg cgatgtgttt
    22921 atcatggggc acaagtttcc ggatatggat gctttaggct ctgcttttgg tgtcagttgt
    22981 ctggcacgct tcaaccagaa aaaagcttac atcgttatca acgagaacga gatcattcca
    23041 gatgtagaac ggtgtcttga agaaatccat aagcatcctg aattagaaat gcaactaatc
    23101 tctccagaac gtgccataga gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac
    23161 cataaacctt ctctatcgat ctctcagggc ctatatgagc aatttgataa gattgtgatc
    23221 attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa
    23281 tcttcagctt cttcagcttc agagctagtt tctgagctga tccaatacca aagcagcaaa
    23341 acaaatcgct tagatcgttt tgaggccaca ttattgcttg cagggatggt tgtagataca
    23401 aatagtttca atgtccgtac aactgcacga acttttgacg ttgccagcta tttaaggtct
    23461 tgtggagcag acgcatcctt agtacaatat ttattgagct cggatttagc gacatacttg
    23521 gagatcagca atttgattgc tggaagcgac tatgtgactt ctgatattgt tgtggcggca
    23581 gggacagacg aaaaagagta taatagtgtt atagcagcaa aaacagcaga tacattgctt
    23641 tcgatgatca atatcaatgc agctttcgtc attacaaagc ggacagacgg cttgattggg
    23701 atcagtgcca gaagtaccgg cacgatcaac gtccaaatca tcatggaatc cttaggtggc
    23761 ggaggacact tcacaaacgc agctactcaa ctggaaaata cgacagttga ggaaacaaaa
    23821 cagaagctat ttgctgcaat caaagaaaat atgaatcaaa tatatgataa ggagtaatcc
    23881 aaatgaaagt aattttttta caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag
    23941 aagttccagc aggttatgca caaaattatc taatcaaaaa taacttggca aaagaagcaa
    24001 cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg
    24061 aaatcttgga agaagctaaa aagttaaaag aattcttgga aaaagaagat actgtagtcg
    24121 aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa
    24181 ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga taaacgaaaa atcgaattag
    24241 aaaatccgat ccgttcacta ggctacacaa acgtacctgt taagcttcat catgaggtga
    24301 cggctacgat caaagttcat gtcgtacaag aataacaaat cttgatagcg atgttcagct
    24361 ggtaagtata accagaagga aaaaagacaa accgaaattt atctgagaaa acagctgcag
    24421 caatgaaagc aaaagctgca aatcgatttc agattgattt cggtttcttt tttgggaaca
    24481 gcaaaatagc ataaagaaaa cgagtataat aaaagagaac tattttttta ttacactttc
    24541 ggtacgaatg atgacttttc tctcattttc ttgtaaaatg aagcaattga agacaaggag
    24601 cgatacgtat gaatgaagtg tggcaggatc gaatcccacc gcaaaatatt gaagctgaac
    24661 aagcggtttt aggctctgtt tttcttgacg cagaaacgat cattgatgcc atggagtaca
    24721 tagagccaaa agacttttat cgtcgtggcc atcaaattat ttttgagaca atgatcgagt
    24781 taaatgatcg taatgaagcg attgacgtgg ttactgtcaa agaccggctg gaacaagcca
    24841 atttgttaga agatgccggt ggtttaagtt atctctcaga cttggcatta gcagttccga
    24901 cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat
    24961 tgatccagac agcaacagga atcgttacga aaggattcga gcaaggagaa gaagttcaaa
    25021 caattttaga tgatgccgaa cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg
    25081 gctttttatc tattgctgaa gtactgaact ccacgattga aaatatcgat cgactagctc
    25141 aaaacaatga agaaatcaca ggtctaccaa ctggctatca aggtctggat aaaatgacag
    25201 caggtttcca gcccgaagaa ctaatcattc ttgctgcacg tccagcggta ggtaaaacag
    25261 cgtttgcatt gaatattgct caaaatgtag gaacaaaaac agatcgttct gttgcgatat
    25321 ttagtttgga aatgggggca gaatctcttg taaatcggat gctttgtgca gaaggttcga
    25381 ttgaggccag ccatttacgt acaggtcagc tttcggaaga agaatggtcg aatctgatcg
    25441 ttgcgatggg gagtctttct cgagcaaata tttttatcga tgatacacca gggatcaaaa
    25501 tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga
    25561 tattgatcga ttatttgcag ttgattgaag gtaacggtaa agagaaccgt caacaagaag
    25621 tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg
    25681 ctctttcaca gttatctcgt ggagtagaac agcgacaaga caaacgacca gtgctaagtg
    25741 atattcgtga atccggatca attgaacaag atgcggacat cgtagccttc ttgtatcgag
    25801 atgattatta tgaaagagaa ggcggagatg acgatgatgg aaacgagcca aaaaatgaca
    25861 acgtcatcga agtcatcatc gagaagaacc gaagcggtgc acgaggaaca gttgaactac
    25921 tgtttatcaa agaatacaat aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa
    25981 gaggttgtga gccttgagca atcccgagag ctctcgtaag aagatgcaag gagtaaccgc
    26041 aggaacgact agagtactta atttaaacag atctccaaag ggattgtgac aaaagtcttg
    26101 tcacagtccc tttttccgaa taaacggtgt tcaatcagct gacccttggt attagctcag
    26161 caaacgtcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
    26221 gctgatctta cgacctcttt tccttaaacg gtgttctcct gccaactcct cggtcacaag
    26281 tcacaatatc atcaaacatg agaagcagtt ttttgaaatt gctccttgtg actcagagtt
    26341 aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat
    26401 acgcccaatc aaacgaaaaa tatgaga
//
LOCUS       DACCQI010000032        26353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCQI010000032 DACCQI010000000
VERSION     DACCQI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.032"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IVO76_001031"
     CDS             complement(99..1229)
                     /locus_tag="IVO76_001031"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7429008.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVO76_001032"
     CDS             complement(1878..3080)
                     /locus_tag="IVO76_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7429009.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVO76_001033"
     CDS             complement(3140..3343)
                     /locus_tag="IVO76_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7429010.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVO76_001034"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVO76_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVO76_001035"
     CDS             4880..5692
                     /locus_tag="IVO76_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7429011.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6913)
                     /locus_tag="IVO76_001036"
     CDS             complement(6716..6913)
                     /locus_tag="IVO76_001036"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429012.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IVO76_001037"
     CDS             complement(7059..7937)
                     /locus_tag="IVO76_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7429013.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IVO76_001038"
     CDS             complement(7976..9094)
                     /locus_tag="IVO76_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7429014.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IVO76_001039"
     CDS             complement(9091..11190)
                     /locus_tag="IVO76_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP7429015.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IVO76_001040"
     CDS             complement(11241..12062)
                     /locus_tag="IVO76_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7429016.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IVO76_001041"
     CDS             complement(12059..12868)
                     /locus_tag="IVO76_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7429017.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IVO76_001042"
     CDS             complement(12889..13383)
                     /locus_tag="IVO76_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7429018.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IVO76_001043"
     CDS             complement(13395..13883)
                     /locus_tag="IVO76_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7429019.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IVO76_001044"
     CDS             complement(13960..16656)
                     /locus_tag="IVO76_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7429020.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IVO76_001045"
     CDS             complement(17084..18157)
                     /locus_tag="IVO76_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7429021.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IVO76_001046"
     CDS             complement(18154..18981)
                     /locus_tag="IVO76_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429022.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IVO76_001047"
     CDS             complement(18982..19788)
                     /locus_tag="IVO76_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429023.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IVO76_001048"
     CDS             complement(19800..20885)
                     /locus_tag="IVO76_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429024.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IVO76_001049"
     CDS             complement(20902..21444)
                     /locus_tag="IVO76_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7429025.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IVO76_001050"
     CDS             21702..22691
                     /locus_tag="IVO76_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7429026.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IVO76_001051"
     CDS             22688..23425
                     /locus_tag="IVO76_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7429027.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IVO76_001052"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IVO76_001052"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7429028.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IVO76_001053"
     CDS             23989..24621
                     /locus_tag="IVO76_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7429029.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IVO76_001054"
     CDS             24706..25353
                     /locus_tag="IVO76_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7429030.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IVO76_001055"
     CDS             25358..26341
                     /locus_tag="IVO76_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7429031.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8061 a         5570 c         4465 g         8257 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gat
//
LOCUS       DACCQI010000033        26177 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCQI010000033 DACCQI010000000
VERSION     DACCQI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.033"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..410
                     /locus_tag="IVO76_001056"
     CDS             <1..410
                     /locus_tag="IVO76_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7429032.1"
                     /translation="NTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSF
                     IAYSQGGIRDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGV
                     WGLIVGIPIFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(536..1677)
                     /locus_tag="IVO76_001057"
                     /pseudo
     CDS             complement(536..1677)
                     /locus_tag="IVO76_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1825..3378
                     /locus_tag="IVO76_001058"
                     /pseudo
     CDS             1825..3378
                     /locus_tag="IVO76_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            complement(3486..4568)
                     /gene="uxuA"
                     /locus_tag="IVO76_001059"
     CDS             complement(3486..4568)
                     /gene="uxuA"
                     /locus_tag="IVO76_001059"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7429033.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(4559..6205)
                     /locus_tag="IVO76_001060"
     CDS             complement(4559..6205)
                     /locus_tag="IVO76_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7429034.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            6389..7051
                     /locus_tag="IVO76_001061"
     CDS             6389..7051
                     /locus_tag="IVO76_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7429035.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            7195..7758
                     /gene="ahpC"
                     /locus_tag="IVO76_001062"
     CDS             7195..7758
                     /gene="ahpC"
                     /locus_tag="IVO76_001062"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7429036.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            7768..9297
                     /gene="ahpF"
                     /locus_tag="IVO76_001063"
     CDS             7768..9297
                     /gene="ahpF"
                     /locus_tag="IVO76_001063"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7429037.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            9454..9804
                     /locus_tag="IVO76_001064"
     CDS             9454..9804
                     /locus_tag="IVO76_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7429038.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            9903..10433
                     /locus_tag="IVO76_001065"
     CDS             9903..10433
                     /locus_tag="IVO76_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429039.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            10587..11738
                     /locus_tag="IVO76_001066"
     CDS             10587..11738
                     /locus_tag="IVO76_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7429040.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(11847..13886)
                     /locus_tag="IVO76_001067"
     CDS             complement(11847..13886)
                     /locus_tag="IVO76_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7429041.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13944..14699)
                     /locus_tag="IVO76_001068"
     CDS             complement(13944..14699)
                     /locus_tag="IVO76_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7429042.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            complement(14791..15921)
                     /locus_tag="IVO76_001069"
     CDS             complement(14791..15921)
                     /locus_tag="IVO76_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7429043.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(16462..16668)
                     /locus_tag="IVO76_001070"
     CDS             complement(16462..16668)
                     /locus_tag="IVO76_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429044.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(16684..17298)
                     /locus_tag="IVO76_001071"
     CDS             complement(16684..17298)
                     /locus_tag="IVO76_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7429045.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(17445..18128)
                     /locus_tag="IVO76_001072"
     CDS             complement(17445..18128)
                     /locus_tag="IVO76_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7429046.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(18237..18695)
                     /locus_tag="IVO76_001073"
     CDS             complement(18237..18695)
                     /locus_tag="IVO76_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7429047.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(18779..20542)
                     /locus_tag="IVO76_001074"
     CDS             complement(18779..20542)
                     /locus_tag="IVO76_001074"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7429048.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(20763..22223)
                     /locus_tag="IVO76_001075"
     CDS             complement(20763..22223)
                     /locus_tag="IVO76_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429049.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(22431..23357)
                     /gene="glsA"
                     /locus_tag="IVO76_001076"
     CDS             complement(22431..23357)
                     /gene="glsA"
                     /locus_tag="IVO76_001076"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7429050.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(23380..24807)
                     /locus_tag="IVO76_001077"
     CDS             complement(23380..24807)
                     /locus_tag="IVO76_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7429051.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(24838..26052)
                     /locus_tag="IVO76_001078"
     CDS             complement(24838..26052)
                     /locus_tag="IVO76_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7429052.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         7705 a         5092 c         4620 g         8760 t
ORIGIN      
        1 taaatacgtt gctcactaca atcgccttgg ctgtatttgg ctttcatcag ctgctgagtt
       61 tagcgatcat gattttcatt ttaagtctga tcccagtagc aggtgttatc atttcttgta
      121 ttcctctatc tttcatagct tattcccaag gaggcatccg agatgttgtt tatatcctga
      181 ttacgatttt gattgtccat ctaattgagt cttatgtgct gaatccgaag ttgatgtcca
      241 gcaaaacaga actgccgatt ttttatacat ttgtggtctt acttgttagc gaacggttct
      301 ttggcgtttg gggcttgatt gtcggcattc cgatctttac cttcttttta gatgtattga
      361 aagtgaaaaa gatcccgaac catcttccaa aagaaacaga tatcccttaa aataaatatg
      421 aagaaagata aagtatgaac gaccccaaat atcagatttc aatctggtat tttggggtca
      481 ttttctggat atttcttttt atttgaaaaa tgcgttcaca gacattattc aaatgttaga
      541 gagatctgtt ttgtttaaat ctttttgcag aaagcattgt tgctatactt aatagaatga
      601 ttccgctcca cagtataact ttgttttgtg actctccgga ttttggtagc tgtttatttt
      661 tcgtttcagg acagcaaagg ttgtatcctt gctacttttt aaggtttcag ctgattgggc
      721 gctatcaaat attcgtgatt ttttattcag ctgctcttca tttattttta gaattgctag
      781 atttgatttt ttactctctt tttttccatc ctcttgaatt gtttttttgt ttattttttc
      841 tggtattcct tggtctcttg cgtccgtatc tggttctgga gtcacatctg gtttttcttc
      901 tggatctacc tctggttttt ctggattttc agtttcatta tctgtgtctg gatttgtttc
      961 cggtactgtt tggtttccag aatccgtatc tggttctgga gttacatctg gtttttctcc
     1021 aggggttatt tcaggttttg atttattgtc tggctctggt tgagtttctg tatcgggttc
     1081 ttctccaggt tctatttccg gtttctgttc tccgtctgga ttttctgtag gcggtacttc
     1141 tggcttattt tcattttctg gtttatctgt tggttcttca acaagatctc ctcctgagtc
     1201 gatggctgaa tcttgctcaa ttgtctgccg ctgactatac ggcgttaaat gatgatcgtt
     1261 cggtagtcca tctgatacct tgacagtaaa aagcgctggt tttaacaagt cctgatttaa
     1321 tgtaacggtg aaggtaaata tcgcttgctt gtgttggata gtcaaagtgt tctcgtcata
     1381 gccaatcgac aaagtgggat caacattctc aaatgtaaca gctgattgta gtataggtgc
     1441 taagtctccc gaatagtcaa tttttgcttt gatggtcgct gtttgtcctt ttcttaattt
     1501 tgcgactcct gaattgaagg atgaaaattc atcctgaaag gtaattcgat tgacgttggc
     1561 ccacttggtt tctgtccatt tggctgtttc tgtctcctct gtgtggactg tctgatttat
     1621 agtaagcgat aagcttaagg ctgcaaatgt tgatattaag atggttgaat atctcataac
     1681 tctctcactc cttgattttt ttgtatgcaa gaaataagat agtagagatt ctacctatat
     1741 gtttactgtt tttttgtcta aatttatgaa aaaaacagta atattttcaa gttttaacaa
     1801 tttaagtaaa aaggagcctc tttcatgcaa actcaagaag cgattaaaac gtttatcttg
     1861 aagaaaaaaa gcaatacaaa attagatatt tttcttttcc tatctgaaca tcgtttcttt
     1921 attactacac aatatttagc agatcatttc catatgtctg aatcaaattt tttactatac
     1981 atcaaagaat tagaacaaga ctttgagcgt ctcaatctaa cagaactgca tattgataag
     2041 caaaaacctt ttttaaagtt gaattttgaa ggaattgatc cggcttactg ctattatcgt
     2101 ctatttggaa gatactgcaa cgaatcagtc agttatcaga tcttgacttc tttgttcagc
     2161 tgtcagacaa attcgattat cagtttcagt cagcaaacaa actatagtgc ctcttatttg
     2221 tatacgaaaa tgaaaaagat taatgcattt ttagcatagt acggtttgtc tatctccttt
     2281 tctaataatg gcagaaagtc tttttctgga aaagaggtac aaattcaata tgcagcatta
     2341 gatatttatt ggaacatctt ttcaagtact gctacagaat tttatgatga agatccgcaa
     2401 gtagtggctt ttatgatgaa tacttttttt aaaaaagaag tacttgaccg actaaatagc
     2461 ggtatgatag ataaattata ttttctatta aaaatatgta aagagaattt tcctgcaact
     2521 tctgtgaaag acgtacaaaa agaattcaca gagtatgaat atattgactt ttttattgat
     2581 ctctctgtag atattttgcg acctaatctg ccaatttgtc gcgaacaacg catcttagtg
     2641 aatattttag ctcgactttc tatcacgaaa atcgaatcgg aagaaacaag cttggagcaa
     2701 tacaaacttt taaaaaaagc aaacgttcct catgttcttt attcaagaga aatggtggag
     2761 tccctttgta ctgtatttga tttgtttata cctgaaaaag aaaagattct ttactcattg
     2821 gcattcgcac gaaataaact ttataatgga tttcttaact tcaatcaacc aaatacgccc
     2881 cttcctactt tcatgcttta tcaagaacat agcgtattga aagaagtcca aaccgccatt
     2941 gaacaatttt atatggattt cagagaacaa aatcaacact tgttaccagt tattttagaa
     3001 aaggaaaatg tgcaatggat ggttgaagac ctagtacatt tgtatgatcg ctacaaaaaa
     3061 cagccgcgaa ttgtgattgg tgtgaactat acgcgtgatt tttatatcag taaagatctg
     3121 atcgttaaga tagaacaaat tttttcaaat gaatcgatca aaatacaaaa tgattttatg
     3181 gagaactgca acatcgttat cagcgactgc cctttgaaac aattgccttc tcatatcaaa
     3241 aaaatctata ttttaaaagg aatcatcaca ccagaagact ggaaaaaagt gattatgagg
     3301 atctcagaat atatctttga attaaaagaa gaagcactca tggatcaatc gaaaaaagaa
     3361 aatcaagtta cttcctagac caaataagat acataaatcc gaatgcttat taacaacctc
     3421 cggatttatg tatcaagaat ttgaacatcc ttcttattta cataataaaa ttacagtttt
     3481 tttcactaat tatctttact tttgtttcct ttttcgattg cttcccataa tccatttaga
     3541 taaacagcac ctaatgctcg atcatataag ccgtatcctg gtcttcctgt ctcattccag
     3601 atcattcttc catgatcagg acggatgtat ccgttaaatc cgttgtcata cattgttttt
     3661 agtatgcggt acatgtctaa tgaaccgtat tcggatttat gtggtgcctc atagaaatct
     3721 cgattagcta aaaatttaat atttcgaaca tgtgcaaatg ggattcgatc tctttggcaa
     3781 aattctgcta aaatcccata tacatcattt tctggatctt ctgcaatact ccctgtacaa
     3841 agagtgattc cgttataagg gctgtctact atgcgacaca accaatctaa atcttctcga
     3901 ttctttacga ttcgagctaa tccaaaaatc gagtatggcg gatcatctgg atgtacagcc
     3961 attttgaccc ctacctcttc acatgttggg atgattgatt ggagaaaata ggcaaaattt
     4021 tctcgaagtt tttcttcgtc tactgatgca taggcttcaa gcaaggattt gataggagct
     4081 aagcgctcag gttcccatcc cggcaatcca aattcattgc tggaatgttc gatgcgcgcg
     4141 acgatttctc ttggatcatc tggaatatca tcagatataa aagctaacgt ggatgagccg
     4201 tcttctaagc ggtagtcaag gtcagatttc acccagtcaa atacaggcat aaaattatag
     4261 cagatgactt ctattccata ttctgccaag tttcggattg tctgcttata gttttcgata
     4321 tactgctcac gggaaggtaa tccaatttta atggcatcat gcacattgac gctttcaatt
     4381 acttttagtg ttaacccacg agactcaatt tcttctttta gttctttgat cctctcccta
     4441 ggccatacct ctcctactgg tatatccatc aatgtgccta caatcccttt gaccgcagga
     4501 atctgtctga tttcatctaa ggtaatcttt tcttcctgtg atccgtacca tctaaaagtc
     4561 atttccatga taaatttcct ccatgttctt gtaagtaatc ctgtaatgtt tttcgtacgg
     4621 catttggtcc tgctaccatc ttgatgaaca tttcttctat ttttctgcca agctctgctt
     4681 ggtaaagatc tacaccaaaa atttctttat ttgacaaaat aggagccatt ttctcatgaa
     4741 tatgtgatgt atcttgcgta cccagttgaa tatcggaaag cttatgctga aggtctgtca
     4801 atagtgggtc tggactcggg atgaatggtt ttccttgatc atcaatgcca ataaggtatc
     4861 gaagccaagc agcgagagtc aatggtatga atacaagtgt ctgcgggtct ttaacttcta
     4921 tttccatata ttttttaatc gtctcaccat aacgaatcgc caccttttga gaggtatccg
     4981 tggcgattcg ctgaggcata ttaggaatat ttggattaga aaagcgcaca gtaattactt
     5041 cttccagaaa gatctttgga tcaatgatac ctggagactc cacaactggg aggccttcat
     5101 tgtacccgat ttcagaaact agcagcgata aatctggatc tttcatctcc tcagctatgc
     5161 tcctataccc cagtaaacaa cccataatag ctaaagacgt atgcaaagga ttaaggcaag
     5221 tcgtcacctt catcgtatct gctttatcta ccgttgcacg atcagtcaag aataccccag
     5281 ctttttcgaa tttcggacgt ccattgggaa aatgatcttc catgattaaa taatggatgt
     5341 cctctgtatt gacaaatgga gcaatattat ttccttttgc ggtttggaaa atcgacatat
     5401 ttgaaaagcc ctcttttgcc aacattttgc taactgacaa ggaaggattc ggggtgatac
     5461 gatcaatcat cgtccatgga aacgcaacaa tttcttcatt cgataagtat tggataaatg
     5521 tattttgtac ataaccttcc ttttgccatt cttttgcgat cgttagcaaa gaagtacgta
     5581 attgactacc gttttgtgaa aaattatctg tagagacaag tgcaatcggc caagctccag
     5641 ataaaaaacg tttataaagt agcacagcaa gaatactcat tgtatgtgat ggcttctttg
     5701 gaccattttt tatatcgact gcaatcggtc ctatatattt attatttgta tctttcaatc
     5761 gataaccttt ttcagtaata gcaaaagtaa ctaattgtaa actaggattt tcaaagtgtc
     5821 gaatcatctt atcaaagtgt tcagaacaat ctggatgaca atacagactg tcagctgtcg
     5881 cagctaatat acgtttgacg aactctccac tgcttggcat gaccacttca agaatcaaat
     5941 catcaaaatc ttggtaagcc ctttcaatta tttcttcatc gaatgtttca caaacaatga
     6001 caccagtgga caattcgcct agatccgcta aacgttgagc gatttctgca tgaaatcctc
     6061 gataaagatt tccgccaccg aaatggatcc agacaggatt tttttgtcca ttttctctta
     6121 atgatgcacg gtcaaaatga ggtatttcta cttctttttg agataattca gggataaagt
     6181 tcctccagtt gtttaaatca atcatttttc ccctcctaaa ctaacatatt agtttttgta
     6241 ttcaagtgaa ttttatgcaa taaaaatcgc tatgtcaacc aaaattttac tatttataca
     6301 aatttactag ttaatgtgac ataccagatt gtttttcaga cctcttctgt tatactacgt
     6361 tcatagaaat gattttggag gaataaagat gaaaagcagc ttgcaatcac aagcttatca
     6421 agcgattcgt aaacaaatta tctatgctga cttagaacct ggaaggaaaa tatctgaaaa
     6481 ggcactggag gaacatcttg ctatcggccg gactcccatt cgcgaagcat tgattcaact
     6541 acgtcagcaa gaactcgtgt atacgatacc tcaaagtggt acgtatgttt ccaaaattaa
     6601 tcttgtttct gcagaaaatg ctcgctttgt ccgagaacag ttggaagagc aaatcatgat
     6661 tgaatgttgt tccaaattgg acacacaaac acgaatagtg ctagaaacga ttttagaaga
     6721 acaagaaaaa gcagtcaaaa taaatgacga acgcgctttt ttccgtgcag ataatttatt
     6781 tcatgaagct tgtttcgaaa ttgcaggaag aaaagaagtt tggaactggt tagatgacca
     6841 taatacccat ttagaacgtt tccgctggtt acgagtgacc attcaggggc ttccatggga
     6901 aacgatcatg gaacaacatt atgatttact caaggcttta gttgataaaa aaccagaaga
     6961 agctcgattt caaacgggat tgcacttaca catgatgctg aaagaacagg aaactgttac
     7021 tcaacgttat cccgagtatt tccagcagta gcattttctt gcgacctctt ctatttttat
     7081 aattaacctt cgtttttgag taatctttat taaataacaa aatatccatt gactttgcaa
     7141 ctccaactaa attacaataa ttataaataa ataattatta ttggaggttt tactatgtca
     7201 ttgattggga aaaaaatcga agaatttaat gcacatgctt atcatcaagg agaattttta
     7261 gaagtcagtg aaaaagatct tatggggaac tggagtattc tttgcttcta tccagctgat
     7321 tttacttttg tttgtcctac tgaattagaa gacttacaag aacaatacag tacattgaaa
     7381 tctttaggtg ttgaagtctt ttcttgctct accgataccc actttactca caaagcatgg
     7441 catgatacat cagatgctat cggtaaaatc gaatacgtga tgatcggtga tccgtctcat
     7501 cagatttctc gtatttttga tgttttggat gaagagcaag gattagctca gcgaggaaca
     7561 tttattattg atccagacgg aatcgttcaa gctatggaaa tcaatgcaga tggaatcggt
     7621 cgtgacgcta gtgcactgat tgataagatt cgcgctgctc aatatgtacg tacccaccca
     7681 ggtgaagttt gtccggcaaa atggaaagaa agtggcgaaa cattgaaacc aagttttgat
     7741 cttgttggaa agatttaggt ggtggatatg ttagacaaag aaactaaaca aaatctcgaa
     7801 caatatttag cactgattga atctcctatt gttttttctg ttagtttgga taatagtgaa
     7861 aatagtcaaa aactagctga atttacgaag gaaatagcag aaatgtctcc taaaatctct
     7921 tgggaaaaaa gagattcgat gcgcacacct agtttttcta tcaatccagt tggcaaagaa
     7981 agtggaatcg tttttgctgg tatccccctc ggtcatgaat tttcctcttt tttactggct
     8041 atgttacaaa ttagtggacg ggcaccgaaa atcagtgaat ctttgagtct taaaatcaaa
     8101 aaaataacac aaacattgca ttttgacagt tatgttagct tgacttgtca taattgcccc
     8161 gacgttgtac aggcattgaa tatattagct gtattaaatc caaatattac tcatacgatg
     8221 attgaaggtg ggatgttcca aaaagaaatc gatgataaaa aaatcatggc agtgccaact
     8281 gtgtttttaa atggagaaga atttagtagc ggacgcatga cgatcgaaca aattgttgaa
     8341 aaaatcactg gccctttgat tgaagaagag ttgtttgaaa aagaacctta tgatgtatta
     8401 gtgattggcg gtggaccagc agctagtagt gcagctatct atgcagcacg taaaggaatc
     8461 cgtacaggac ttgtcgctga ttctttcggc ggacaagtcg ttgaaacact agggattgaa
     8521 aatatgattg gtacctctta cacagaaggt ccaaaattga tgcagcaagt agaaagtcat
     8581 gtgcgtagtt accctatcga cattatgatg aatcaacagg ccgtttcctt aaataaagag
     8641 caacatttta ttaatattgg cttagctaac ggtatatcac ttaaagcaaa aaccgccatc
     8701 atcgcgacag gtgcgcactg gcgttctatc aatgtaccag gcgaaaaaga gtttaaaaat
     8761 aaaggaattg cttattgccc acactgtgac ggccctttat ttaaaggaaa agagattgct
     8821 gttatcggtg gtggtaattc tggtgttgaa gcagcaatcg atttggctgg gttagcaaaa
     8881 catgtatatg ttctagaatt cttaccagaa ttaaaagcag atcaaatctt acaggataaa
     8941 ctagtctctc ttgcgaatgt cactgttatc aaacatgcag cgacgaaaga aatcagcggt
     9001 acgaatcagg tagaatctct ctcctatatc gatcgccgta cgatggagat gcatacacta
     9061 gctgtttcag gagtattcat tctagtcggc ttactcccta atacggattg gctagatgga
     9121 acgatcgaaa tgaatccaag aaatgaaata gtgaccgaga aaaatggcgc aacgaatatc
     9181 cccggcgttt tcgcagctgg tgattgtaca gatagtccat acaaacaaat cattgtttca
     9241 atgggttcag gagctactgc agctttagga gcattcgatt atctgatgcg cacttgagct
     9301 atgaagattg taaaaagtca gagagcggaa aaaaggatgg tgacaagaat tttgtcacaa
     9361 tcctttttgt ttttttgaat aatcgctttt tcttctcttt ttcttatata ctataagtag
     9421 taaataattt gaaaaaaaga aaggtgactg attatggcgc aaaatcgtat ttttacgaca
     9481 agttttgcaa gcgtttatcc tatgtatgta gcaaaagcgg agagaaaaga tcgtataaaa
     9541 gaggaagtag atgaaatcat ttgttggctg acgggttatt cccaaacaga actagaatat
     9601 caactacaaa aacaagtgac tttcgaacaa ttttttgcgg aagcccctgt ccccaatcct
     9661 tcacgttcct tgattacagg tgttgtttgt ggtgtacgag tagaagacat cgaggataac
     9721 ttgatgaaag aaattcgtta tttagataaa atgatcgatg agctagcaaa aggcaaaaaa
     9781 atggaaaaga tcctcagaag ttgatttttc cagagatatt cttctccttc gctcattttg
     9841 taacatttaa tttggtaaaa tgactagttc acgttaaaat aagtcaaagg aggttttctt
     9901 atatgtttga gacgattaaa caaaatattc aaaaatacgc cttattaaga gctgctgttt
     9961 atattatttc tgggattgcg attgtgctaa acccttctgc tgtttttcat tttatcggtt
    10021 atctgatcac cgcctatctt gttttgcttg ggttgttgaa tctttttgag gcttaccgtt
    10081 ttaaaaggaa aacaggttct tggagtatcg gtctgttcag tggtcttttt ttcctgatct
    10141 tagctctgat cgtctatgct tttgcacctg caatcgtttc tatcctgccg attcttcttg
    10201 gattagctgt tatcatcaac agcttgttcc agttgttctt cagcatgaat acaaaagcta
    10261 aaagttggag tgtttatagt agtattctgt tgttgattgg cggattcgtc ttattgttca
    10321 atccattcaa aagtttgatg cttctattcc aaatattcgg ctttattttg attttcatgg
    10381 gcatccagga gattatcaat ttcatcagaa taagaaaaat gactatacgt taatcatttt
    10441 tagttattaa aatagaccgt ataaaaacca acaatccaaa aaaatctttt ggattgttgg
    10501 tttttttgtg agtatatttt cctatttttt caataatgta tgttatgcta agttcgggct
    10561 atattttata caaaggaatg atgagaatgg aatttattgg tattttatgc ctgatattga
    10621 tttcaacaac gattggctca catatctcgc ggagattagg tatcccagca gtgattggac
    10681 aattacttgt tggtgttttg ctaggccaag ctggattgaa ttgggtcaaa cctgatattc
    10741 tagtccatga cttttcggag attggtgtga tcttattaat gtttttagct ggtctggaaa
    10801 gtgatttatc tcttttgaaa aaatatttcc gacctggtat gttcgttgct ttgcttggga
    10861 tcatcttccc ggttgttttt ggctggttga caggcaaagc attccaagtt tcgggaaatg
    10921 aagcgatttt ctttgggatt attcttgcgg caacttctgt cagtatttca gtagaagtat
    10981 taaaagaact aaacgtagtg aatacaaaag aaggatcaac aattttgggt gcttctgtgg
    11041 tagatgacat tttagtcgtt ttagttctaa gtttcagcct atcttttcta acgggggaag
    11101 cttctagcaa tgcttcttta ccgcttgttt tgattgaaca gctaatttat tttgttttta
    11161 ttttcttatt agtcaaatgg attgcgcctt tcctactttc atttgcagaa aaagtgtacg
    11221 caaattcttc tattatcatc gtatcgctag ttatttgttt aggaatgtct tacgtagcgg
    11281 atcttgttgg gcttagctct gtcattggtg ctttttttgc tgggatagct gtcagtcaga
    11341 caaaggttaa agaagaagta taccacaatg tagaggcttt aggatatgca gtatttatac
    11401 cagtattttt tgtcagtgta ggattagaag ttgatttttc tgactttagt gaacaactac
    11461 tctttatttc tatattgacg cttgtagcga ttttgaccaa attgcttgga ggttattttg
    11521 gtgctcagat tgccggtttt tctcaacata gttcattgat ggtcggtgct ggtatgattt
    11581 cgcgtggaga aatggctttg atcattttac aaattggaca gcaaagtcaa ttgatcgaac
    11641 cacagtacta ttcaccttta gttatcgtga ttttattaag tacacttatt tctccacttg
    11701 ttctcaaata ttttacaaaa aaagtttatg aagtgtgagt aaatcaaaaa gaagttgtga
    11761 aagagacatc gtgaaaaccg tttatttgaa aaaaggactg tgacgataac gccacagtcc
    11821 ttttactgta cttaaaaagc aattttttat tgataatttt ggttgaggta ttgcaatagt
    11881 tccgatgctc gtttggttgc tgaatcatca tcttctttat tttccaacat gctgaccatc
    11941 atataacctt ggttatcaag attgaaagca aacaagaaac tgttctcttg cccctttaca
    12001 tcctgttttt ctttgatttc cgctgtacca gttttcgctg ccaacggaat ccctaaagca
    12061 gaaagagaat gtgctgtacc atttacatct tgcacaactt ctctcagatc tggcacgatc
    12121 gtttgtactg ctgtttcgct gatgacattc ttcttatctt ttgtctcttt atctgcaatc
    12181 aatttaggat agacaagtgt gccattgttg gtaaaaacag aatacattgc tgcttgctgg
    12241 ataggagtaa ttagaagttc gccctgtcca tatccagtat cagctagcaa gatatctgag
    12301 ttaaagctat cttcattaga aatttgtgct ggattcatac taattggcaa atcaaggtct
    12361 tcaccaaaaa tgaatttatc taagcctgta cgaaattttt tctcacccat tttcaacgtt
    12421 tcttgtgccg catatatatt atcggaatag atcaaagcag tttttaagtc tacttgtgat
    12481 acatcactac tgacacgcgt tgcttgataa gatccccaag aactgtcttt ttgccattta
    12541 agcccgttga tcgtcaacac ttcatttgga tcgatagtgc cgttgtcgag accgattgct
    12601 gctgtaatca ttttaaacgt agatccagga gcataacctg tcgcaaatcg gctgatgaat
    12661 ggatgttcag gattttcttc ataagctttg taatcttctt gtgagatccc gtttgtcatt
    12721 ttgtttggat catagcttgg agagctagca agcgcaagaa gatcaccggt ttttggcgtt
    12781 gtcgcaacag ttgatccagc ttttcctcct agactgtcaa aagctgtttt ttgtgccttc
    12841 gcatcgattg tcaatttgat atctttgcca ttttgaactt catgttcgat cagaactttt
    12901 ttctctacac cgtctgcatc tgtaatgctt aatttcccac ctgttgtccc acgaagatcc
    12961 ttatcaaaag ccatctccaa acctgagcga ccgatttttc cattactgct taattctgga
    13021 ttcttatcaa tatcttctgc tgtgatatct cccacgtaac caatcagctg agcagctgct
    13081 tcacccaaag gataatatct gccgtctact tcttgaatgg tagctccagc tggaagttct
    13141 ggcgttgctc catcaatgat tttcaatggg acaaagtaat cgggttgtac ccagctttgt
    13201 gaaatcgcct gattgatagc atcttctgtt aagtcgaagg aggaagcaat cgctttgata
    13261 ttggctgttt tttcgcctcc atctccaagt ttacttggaa cgactcctaa ttgttttagt
    13321 ttgcctgttg ttgctaatgg ttcaccattt cgatctataa tattccctct tgctgcttct
    13381 tgtgtggtca ggcttacttt gtcatttcct tccatttctg gaaaaaccaa gttgggttgc
    13441 cagttgatcg tggtttgacc atcatttctg tctaatgttc ctttataaga aagatctttc
    13501 aattcaccta atgaggtatt catctttgct ttatatgaaa aactgtaagt agaatcgtct
    13561 tttttatcta cttttagatt cgatatctga agtcctttga catcagcagc accgtatata
    13621 ttctggtatt tatctaagat ctctttttca gataatgcac ttttatttgc cgcctttttt
    13681 gatgtcattt ctgcagcttt gttataatct cccttgttca aagcttggac aaattgctca
    13741 actgtctttt ctccagcttc tactgcttgg gtttcttgat agtgctggta gtaaaaataa
    13801 ccgccacccg cagctgctat taagatcact gcgccggcaa tataagcgcc tgttcgattt
    13861 ttgccgtgct tgtcacttct tttcattttt tcctccttct cttgcttgtc attcctttat
    13921 ttcactattt atacctgttt attctaacat atgtctttga ggaaatccat accacaatcc
    13981 agaagcacac tttctggaag gttcgtttgg atactaccaa gtaatttccc aaccgctttg
    14041 ggtagcttga atattgaagt tacgtctttc agctgctgag agatagcttt gatcacttgc
    14101 tgctgccttc tgatcctccc gaagtctcct tctttgtctt cccgaaatcg agcatactgg
    14161 agtaaagtat taccatccat cacttgctct cctttgttta tatatacgcc atctaaatct
    14221 agagattttt ctgcatcgat cttgactcca ccaggaaaca gttcattaac gcaatcgata
    14281 aaatcttgaa atgtgatact tgcataatag ggagcttcaa atttgaaatt ttcctttaat
    14341 gtttgattta ataaatcaac tcccccataa gagtaagctg catttatctt attcattccg
    14401 taacctggaa tatcgacata tgaatctctc atgatagaga tcagttttgc ttgttgtgtt
    14461 ttctggttgt attgtgcaac cagaagtgaa tccgagcgac cgttttcgtc tttcgaacga
    14521 gcatctgaac caatcaaagc cacatttagt gtggtaggga tacttttcgg ctctaccttt
    14581 ttcacagttg aactctgttt tgcagacgaa aatccatgga taaactcaat cgaaaaatag
    14641 ccaactacta atgtaagcag taacaaaata gataatatga ttttttgaga tactttcatt
    14701 gcgaattcct ctttctaaaa gaacctctcg cttgaaaaat aaaagattgt actttatgat
    14761 aattcaaact gagagtttct cagtttctct ttattgtttt tcaggtcgat agagcgccaa
    14821 ctcttcggcc tgtatctttg aagaaatatt caacgtgatt ccgattccta aagaaaggat
    14881 cagataactt gttcctccgt agctaacaaa tggcaatggc acaccagtca ttggcatcag
    14941 ccccgcgata cttcccacgt tcattatcgt ctgaacgaat agcaaagttc cgaatcctaa
    15001 aagaaacaat ccagcttgct gatttttaca gcgactgctt aaacaaaaga ttctcatgca
    15061 taatgagaac aataagaaaa gcacacacaa tgcaccgatt agcccaagct cttctgtaat
    15121 aatcgaaaaa ataaaatctg tttctgtctc tggaagatac cctttttttg tgatactatt
    15181 tcctaatccc cgcccaaata tgccaccatt atatagtgca taaaacgaat gggtcatttg
    15241 atagcccgca ccatgactat cgataaaaga atctcttaaa gtcgcgatcc tttcataagc
    15301 atgattaaac atctgtggaa gccaaccttt atttcctaaa tataataccc cgcctgctgc
    15361 tccaatcagc aatgcagaaa aagtaacgat tagatagatc ccttttttaa atggaaccgc
    15421 cgctgcccaa aatatgaccc acgcaatcga aaggatcatc aatgctccag caatctttgg
    15481 ctgaaataaa atcagaaaag ttatacctac tgtaatcagg aatggttttt ttagatcttt
    15541 tgggctacta tttccgtcac gaaaaaacca gcttaaataa aaaatcaaaa aaagatttgc
    15601 cagttcagaa ggctggaatt gaatgccaaa cagagagatc caacgttgtg cgccgttgac
    15661 agtgacaccg aatatcccta ttcttactaa tactaaaaag aaaatcgata aggctaaacc
    15721 gtatcctgct attttagggt gaagtaaata gtgtagtttg actgaacgag ctaagaagat
    15781 cacgctccaa ctcaaaaata tgaaaattag ttgacgcaat aatagacttt ttgtattttc
    15841 atcggcttgc aataaacgat aagaactggc actgtatact tctaaaagac cgatcatgga
    15901 caaagtcaaa taaggaccca aaatccacca atcgatcttt tttcaccgca tctcctcctc
    15961 acacagcaat ccacttgtca ggtgaggagc gagtttgtct catcaaataa gtcagtcaca
    16021 aaaatataaa gaaaaggtca gaaacctttt cgaacaagaa ttctgaccta gcatcatctc
    16081 atcgtccgag ttttagcact atacaacgta aagaaaacat tcttagctat tggaaaaagc
    16141 cttaatccaa cagtttctgt tttacccatt ggcgactttc gacgtttctg ggcaacagcc
    16201 tatgtttgta taggagcctc acctaacaag gttattacta tattctattt acgagttcat
    16261 gatagacttt tcctaatgat taggcaatgg aatttgaaat gaaatagtga aatttaacaa
    16321 tctttaatat tttttagaaa aaaataacaa ataagttgaa agaatcccgt tgcaaattct
    16381 ttcagttgca ccaggattct ttgataattt tttagtttta tgtgtttttt tatatgtttt
    16441 ttcacttttc ggaataaata tttatttgtt aaagtaatta aacaatccgc taatcgtttg
    16501 atctggcatt tctatttcct taccatctaa agtaacagta gtaacgacaa aatggtcttg
    16561 caatggcgga acttctaagt actgcccttg cagaaaatca ttcactgttt catattcttt
    16621 tttcatttct tttgaatctg tcgatggttt atagcttatt tttaacataa ctagtgctcc
    16681 tttttattcg ggacgtgcaa tcgtgaaaat ttctcctatt gctgcataat catttcctgc
    16741 caaacggctt acagcattca attttcttgg atcgatcctt cctgtcttat catcatagac
    16801 tgtttcattt aaatgatatt tctgtacttc tagcaaaaat aaatcagctg ttactttatt
    16861 tttattccgt atctcgacat gttcatataa ggatgtttca aaacgaactt ttgcttctgt
    16921 caatgctggt actgaaatga ttactgaatc agttaaagtc aagtcagtca ggtacagttc
    16981 gctttcctca ttaggcagat tggctgctgt tttatttact tcttctacgt tgtcctcatc
    17041 aaggatatgg ataacagctt ctcttgtctc aagaatattc tttgctgtgt cttttactgc
    17101 atcttgtttt ctctggatcg ccaaggataa gatgggtcga ttcgaactga cgatattgaa
    17161 gtaactgaag ggtgcgatat tcactatatc ttttgctgat ttagtcgtta ctactgctac
    17221 tggtctaggg atgactgaac caatcagcaa cttgtaattt tcacgttctg tcaattctga
    17281 aggactccgt tcaaacatgg tttcgccttc tttctataaa attcgttaaa aagcagatct
    17341 tgaagacaaa atgaaaagta atgctttttt catcttctta gaatctgctt taaactatct
    17401 atcaattttt cttttttgtc aagaatctgt gccgtgcgtg ttttttagtt atttgcttta
    17461 cttgcggctt cgacatatct tttagtgatc aattgcggac gggtgataat agatccaact
    17521 acgactgaaa aagcacctaa ttcatatgct ttctctactt gttcaggaga gtcagtatgt
    17581 ccttccataa ttactgggat atcgacttgt tctatgattt gattcaataa atcaaaatca
    17641 ggtgtctgcg tctttcgtga atattctgta tagcctgcta aagtcgtaga caaaagatca
    17701 aaaccatctt cttgcgcttg aagcgcttcc tcaaaagtcg aaatatctgc catagctaac
    17761 cgaccgtgtt cgtgaatcat ttggacaagt tcagcagttg ttttttctgt cggacgttta
    17821 cgagaagttg catccagtgc aatgatttct gcttgcgtct ccaaaagttc ttccacttct
    17881 ttttctgtcg cagtaataaa cacttctgac ccatcgtaat ctcttttgat aatccctatt
    17941 accggtaaat caacgattcg cttgatttca ttgatatctt ctgtcgtatt tgctcgaata
    18001 ccaactgctc cgccttcttt tgcagccaaa gccattctcc ccataataaa agaactgtgt
    18061 agcggctcgt tttccaaagc ttgacaagaa acgacgactc cgcctttgat ttgttccagt
    18121 gtatccacaa tatctcctcc agtaatgtta tataaaaata cagcttcata taaaaatgta
    18181 acccttttct attcaggaga caataatgag ctatttcttt cagcagacct ttcttactaa
    18241 aagaaaatta cttcaaattc ttcgtatacg accagcttct cttctgtaaa ttctatgttc
    18301 aacgcttctt tataagcatg tttaaaaaac tcagcatgga ctttccgcca ataagctaat
    18361 gtccgatccc cttctccttc tttccaagca tgctctttcg tgacttcact aaaaggcaag
    18421 atggtcacat ttatattttt gatgatacaa actggttcat tttttccatt caggaccacg
    18481 tctaattgac caacttttgg taaaggctca ttctcttgtt tatacagatc gtaaccactt
    18541 gatgtaccag tctttatctt ttttacaact agttcgctca actcgtctgc cattttttct
    18601 gtatccccaa atgaaaagaa acctaatttt tctgattgat aactaggata tttttcttgg
    18661 aactttttga catattcttc tacttgctga tacataacat ccaccccttt gctttttata
    18721 atgaaaaaac catccagata ctagtataaa caattatctg gatggtgagc ataatgaatt
    18781 attgaatatt caagacacgc agactatctt caacgatttg ttttggtgtt aaatgataac
    18841 gttcataaag ctgccctaca ggaacgctat ccgtgaattc tttctctgca ccaaaattta
    18901 atacgttcat tgaagaatca ccataataac gactgatatt ttcgccaaat cctcctgaca
    18961 aactaccatc ttctaatgtg atgactaatt cgtgtgtttc ttttaattta tctaatgttt
    19021 ctacgtcatt ttctgtgatc gttcctggat tgatcaacgt tgcttcgatt cctttttttc
    19081 taaaagcttt tactgtttct tttcctaatt caagaaaacc gcctaaagct aaaattgcta
    19141 cttttcctcc attatttact gtttgatagg ctggaatact ataatcattt gcgatagctg
    19201 taccatgttc tacaccatgt gtaggaattc ggattgctac aggctgcgct gtttgttcga
    19261 acgcccaatc tagcatagag atcatatcct cttttgttgc tggagcaaga tatttgatgt
    19321 tcgggatact tgataagtaa ggaatgtcaa atgttccttg gtgtgttcgg tcactagctg
    19381 agatcgttcc gccaccgact aaaataatca ctggattctc gttgatcgct aaatcatgtg
    19441 ataattgatc atatgcccgt tgcaagaaag tacttgattg gaagacgact ggacgtactc
    19501 cctgagaagc caaagcagaa gcgaaagtga tagagtgctg ttcagcgatc cccacgtcaa
    19561 aataatgatc tggatgtttt tcttggaatg ttttcagtcc aaaggcgcct ggtatcgctg
    19621 cattgactgc ggcaatagga gctgtacttg tttgcatttt cttatccaaa taatccatga
    19681 tgatcatatt aaatgtttct ccagaagtta cttgctttgc ctccccggtg gacagatcaa
    19741 atggtacatg ccaatgatat ttcattttat tatcgattgc cggctgataa ccgcgacctt
    19801 tttccgtatg gacgtgaagt acaatcggat gatcaatatc ttttacttct tcaaataaac
    19861 ggatcaccgt ttccagatcg tttccattat ccacgtaacg ataatcaaat cccattgctt
    19921 taaataggtt attgctgctt ttaccgttgg tttgacgaag ctcagctaag tttttatata
    19981 aactgccatg attctcatca atagacatct cattatcgtt tactaatacg attagattgg
    20041 attgtaaaac accaccattg ttcaatcctt caaaagctaa gccaccactt agggagccgt
    20101 caccgataaa tgctaccaca tttcctgatt ctttcttcag atctctcgct ttagctaaac
    20161 ctgttgctaa gctgatagat gtagatgtgt gccccactgt aaaatagtca tgtttacttt
    20221 catgttggtt ggaatatccg ctcacttcac ggtaatgtcc tttttcaaaa ccattttttc
    20281 gaccagttaa aagcttatga ccataagact gatgggaaac atcccaaacg attttatcct
    20341 ttggtgaatc aaatacataa tgaaacgcaa tcgttaattc tactacacct agattcggac
    20401 caacgtgccc acctacatcg ctgtcctttt gcaagatttt ttggcgcatc tcttctgcca
    20461 actgtttcat ttcctcaaca gataattttt tgacatctgc ggggctattg attgcatcca
    20521 atacatgtaa tactgtactc ataatacatt ctctcctccg tactatcttc ttttaatgaa
    20581 aatagatttg ttacacttta tctattctac tacttagagt tgactctaag tcaatagcat
    20641 tttaaaaaaa tttcagagaa agcttgttat ataagtagat cttcgcacga atcaggactc
    20701 aaataaaaac cttctacaga caattaaaaa gctttgtaga aggtctccta attaaggtct
    20761 catcaagaaa tatgtttttt taataggtga agttcctgag caatatgata caaatggatg
    20821 tttgcacctt ttaatgagat tggatagatc tttgtctttt cagtttctaa tccattttga
    20881 tagctagcag catcgcacaa aaccactctt tttttcgcac atagacattc atcaggcaat
    20941 tcttctaatg aacgaaataa aataggattt atttcagcga tgccttcttc cgcatacaaa
    21001 tgaaattggg ttcgataaaa aaattgtttg actgaacttt ctgaaacaac aaaaatttct
    21061 atttcctgtc tccccgcttg ttcactgttg atcaaagaaa gacttttgac gaattttcga
    21121 atgaaatttt cattccatct tagttggatt tgatttttct ttttccactc ttccaatatt
    21181 tctacaattt ttccgtacag aagttgttct ttttctgata gaagatagat cctttccggt
    21241 aaaaatactt gtccatctgc ccacaacgta cggacaaatt gtaaaaagga ttgcttgaaa
    21301 ataaaatttt ctgcatcgtc gatttcagcg atactaataa tctgtaaaat caattcattc
    21361 agtgataggt cattgagaac aaaattttta taaacaactt cgaaatcttt tcttaacagc
    21421 tccatgttct cattggtata atttctactg ttgaacaaag aaaaaatata atagacatct
    21481 tcttcttgta agtctagaat tcctttttgc tcttccaatc cttgacagac taattgaaat
    21541 aacggcttcg tttctgcgct tttcttatct tcttctttca gtccaagggg aaatgccttt
    21601 cttccgattg cgatctctat tcctcgtatg ataaaggact tttcatcttc cgaatatctt
    21661 cttttaaaaa aatgagtctc tacataatca atcaactcgt tacaagcttc ttctacatac
    21721 ggatttttat tttcatagcc ttcccaattg acataacgta ctaaagctag caagatgttc
    21781 cgcgagtcaa attctgggat aatgatttct ttatttttca aataatcatt ttctttaaaa
    21841 aaatccaata cgattttttt gattgtatat actttgctca aagaaataaa ttcaagatca
    21901 gccagttctg tactcgtgaa tataccatta aaataataac ttaaaaactt taaaaaataa
    21961 gattgaccgt acagaaactg tgtgagctca gaaagagcaa taccatgttt gaatacgagt
    22021 ttatacttat gcttctctct tacttgttct tttatacaat attctagaca ctcggctaga
    22081 ctttctagat cgtgaaggat cgttggtgta gtcaccctta gagaaatagc tgtttgttcg
    22141 atgtctattt cttggaattc aaataattgt tcacacagat acacttttct aaatatattt
    22201 cgctcgatgt aattttccag cattcctgat acacgcctcc agtttttctt atgattttat
    22261 ttaatcatat ctgtaaatcg tatttttgtt tcctttttat ttagaaaagc cctttcaaaa
    22321 accaataaaa aaagaaactg ggacaaaagt caaacaagct cacaatttca gcctcattgt
    22381 aggtagcttt gagccgtttg gacttttacc ccagcctctt tcaagaatgg ttattcaaag
    22441 atgtcagcgt ctagttcttt gactacatct ttcaacaatt ggataccagc tgcgctgtta
    22501 ccaaatggat ctagtgccgg gctgaagaca cctatgccgt aacgatttgg cacagctgcc
    22561 atcaacccgc cgcctacacc gctttttgca ggtacaccaa catggacgga aaactctcct
    22621 gattcatcat acaaaccagc agttgtcata attgatttga cgattgtagc actttcttca
    22681 gtaatgattt gtttgccatt ccaaggagca attcctttat ttgccagcac agcagctaat
    22741 tttgctatgc ctgtagcggt gacattgagg gagcattgtc tgaaataagc atcgagtatc
    22801 tccggtacgt ctccttcgat catttgatta cctttcatat agtaagcaag agagcgattg
    22861 atatcacctg ttcttgattc agaatgatag atttcttcat tcaatgtgat attaggatca
    22921 tcacagatct ctttcataaa tgacaagatt cgctcaaatt tttcatctgc atctttaccg
    22981 gcaatcaacg aggttgttgc aatcgctcct gcattaacaa aaggattcat tggatgtttg
    23041 cgatgattga tttccatatt catgatagaa ttgaaggcaa aaccggttgg ttccgtgttg
    23101 attgtttgaa agactttttc tattccgttg tctttgattg ctaataacaa tacgggaact
    23161 tttgacatgc tttcgatggc aaagcgcaca tcggcgtctc ctccctcaat cagttgtttt
    23221 gtatccaaat cataaatcgc catacctaat tgtttgggat ccacttcagc taatgctgga
    23281 atgtaatccg ccacttgtcc tgtttgataa tagtggaaat tttttccaat caattgttct
    23341 aattctttta cttccatttg aataaccagc tttcattttt tatttttctg catgctggaa
    23401 tttatgacgg catgcgtaga tcgtaaatgg aataactaag acgataagat aagaaacaat
    23461 cagaatcgtt tcataaatcc catgctgtcc agctgcgata ctagcaggtg gaacaaatga
    23521 gatacaaaaa gcaaaaagtg aagtcaaaag acctaaacaa gcaacgatta cttttacaac
    23581 ttttccgcct ggaacctgat aggctctttc aagactgtca tctttaaaaa ccagcttcaa
    23641 atagcccgcg aaaaacataa agtacaacat caggtagatt acaacagtca atgacatcgc
    23701 tgcaagaaat gaaagattat tccccccgcc gcttaatgta aagacggctg cccagattgt
    23761 tgcaacgatc ccatttgcga tcatcaaatg agcaggaaca ccgtgtgtgt tttcttttgc
    23821 aaaatattca ggaagtaatc cttcttctgc tgctacgtga agtgcttttg aaggagaggt
    23881 tacccaagaa gcaatttctc cgatgacacc taaacaaaca agaatcgcaa gaattttcgc
    23941 aatccatgca agatttggat caatgtgtct taataggaat tctaacgatt gaatcacccc
    24001 tgtattaaga gacaaaccat gttgaggtac tgtagtagcg acggtcaatc cgccaaaagt
    24061 atctaaaatg attgccagaa taaccaatac aaacattgct aatggataat cctttttagg
    24121 gttttccatt tcatttgcat gactggcaga tgtttctacg cccatatatg ctaaaataaa
    24181 ggaaacaaaa acaaccagcg taggcaattt cgaaaaatca ggaatcaatg ttttcataga
    24241 cagtatcgtt tgtagtggag cgcctgaagc gacataatgg atccctaagc caaacaagac
    24301 taatgctggc aacaagatac cagctacaaa accgatccga gcaatcaatg aagtgtattt
    24361 ggttcctttt aactgtgaga gtaaaatagc ccagaaaata atcaatagga ttgccaattt
    24421 cagatattta ttgttttgta tagcaccaat acccgttgca taggataatg caccagttaa
    24481 gaaatacagc atggtcaagt agccaactgt tatctcaaac cattggaaaa agattgcggc
    24541 aaaaccgaat cgttttccca atgtgcgact gacccaagta tagacaccgc ctttttccca
    24601 tcctttcacc gttgccatct ctgctgcgca caatgccaca gggataaacc atagcaaacc
    24661 gcctaataac aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg gatattcata
    24721 taatgaaacg accattgaga tggtcatagc caaaaagcca aagaacgtta atttttttgc
    24781 aggaattttc gttttgcttt gttgcatgaa tcttcctctt ttctatttga atttgtttca
    24841 attgagtttg ttgccaaaga agaaaaagtc caatccttct tcttgttctt cttttgtagt
    24901 agtgatcgtt gtaaagtgca tcgccgattt taaaatcact gttgtcagca cataggcata
    24961 cacggcagta atgatgacac caatcacttg aatccctaag aagtaccagc tgcttggatt
    25021 cgttagtaaa tggctgttag atgcaaagat ccctatcaga atcgtacctg tgaatccgcc
    25081 catgccatga acaccccaca catcaagtgc atcatcccat tgtttctttt tacgaaaatc
    25141 aacagcagca tgacagacga tacccgcaat gatcccaatc agcatggcac tttttgcttc
    25201 tacataaccg gcacaaggcg taatggctgc caatccagca acggatcctg ttagtacgtc
    25261 gacaaaatca aagccatttc cttttacttt tgcaatgatc agccaagtaa tcatcgcaaa
    25321 tgcaagagcg atgaatgttg aaccaaatgc attgactgct tgttcatctg ctcgaagtgc
    25381 accaccggaa ttaaaaccga accagccaaa ccaaagcagt cctgtaccga tagcagctgc
    25441 cataagactg ctatgctgac ttctttctcc taaaggaatc tttctttgac caagtgtgag
    25501 gatacatgct aaagagccga atccagctgt cgtgtggatg actgctccac cagcgaaatc
    25561 tctaaatccc atctgatcca aaaatccgcc accccatatc cagtggcaaa cagggaaata
    25621 aatcagcaga ttccaaaaaa taacaagtat aatatagcct tttaaattca atctccctgc
    25681 aaaagctcct gtcatcaaag gaacggtaat cacgataaac atcagttggt aaagaaagaa
    25741 cattaaaaat ggaatcgttg caccatgaaa catgttgggg aagcttgtaa catgtctcat
    25801 caaaaagaaa tctgccgggt ttccaataac accgccgata tcccgtccaa aggccaatcc
    25861 gaatccacca aatacccaca taatcgttgt tatcccaacg gagagaaaag attgagacat
    25921 gatcgttagt atattattct tccgtgctaa tccgccgtaa aaaaaagcta gaccaggagt
    25981 catcaggcaa accattgctg tacataaaat catgaatgct gtatctcctg tattgatagg
    26041 taatgtattc atttttttcg cctcctttct acgattacga gtataaaaga aggtgtagtt
    26101 gtttttttct tctgtttttt tgaattaata cttgcattta taaaagacag acaattaata
    26161 aaagcttgat gtgttgt
//
LOCUS       DACCQI010000034        26032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCQI010000034 DACCQI010000000
VERSION     DACCQI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.034"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..595
                     /locus_tag="IVO76_001079"
     CDS             <1..595
                     /locus_tag="IVO76_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7429053.1"
                     /translation="FFKRMIDIVGSIIGLILASPLMFYVAYRIRKEEPGSPIFFCHTR
                     VGKNGKTFKMYKFRSMCLDAEEKLEELLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSI
                     DELPQLWNVLKGDMSLIGPRPPLEREVAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSF
                     HKMVELDLEYISHRSLMYDLRLILKTFYVIVKPNGAY"
     gene            1822..2502
                     /locus_tag="IVO76_001080"
     CDS             1822..2502
                     /locus_tag="IVO76_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7429054.1"
                     /translation="MIGIEEKKGVQKDVTIDREENLRGHRFYWIGRRAQDIFFSALAL
                     IALSPVMLITALAIVIDDPGGSPIFVQDRVGRNGKLFKFYKFRSMCVDAEAKLEALLA
                     QNEMDGPVFKIKDDPRITRVGKFIRKTSIDELPQLWNILKGDMSIIGPRPALPREVEQ
                     YGDYERQRLFVQPGLSCYWQIAPHRNELSFEEWVELDVKYINERSFITDWKIIFLTLQ
                     AVLFGYGE"
     gene            2531..3844
                     /locus_tag="IVO76_001081"
     CDS             2531..3844
                     /locus_tag="IVO76_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7429055.1"
                     /translation="MNIILLSGGSGRRLWPLSNDIRSKQFIKLFKDENGEYESMIQRV
                     YRQITTVDPDAKVTIATSKSQASAIHNQLGDKASICVEPCRRDTFPAILLAAAYLHDE
                     LGVGDDESVAVCPVDPYVDNSYYEGVEALEKLVQQNTANLTLMGIAPTYPSEKYGYII
                     PESSNEVSTVKEFKEKPDKATAEKYLAQNAVWNAGVFAFKLGYLLEKAHAMVDFEDYR
                     DLYSKYETLTKISFDYAVVEKETSIQVMRYNGDWKDVGTWNMMAEVMADNIKGKAVMD
                     DTCVNTQIVNELNIPILAMGCKDMVIAASGDGILISDKERSGYMKPYVEKISTEVMFA
                     EKSWGTYTVMDVQEGSMTVKVSMQAGQKLSYHYHNSRDEVWTVVAGQGKAIVDGMKQI
                     LRAGDVVTIAAGCKHTIEAVTNLDIIEVQIGETISVADKYKSSLN"
     gene            4650..5417
                     /locus_tag="IVO76_001082"
     CDS             4650..5417
                     /locus_tag="IVO76_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAP7429056.1"
                     /translation="MGIEGGYLMSRMRFMNTEIDNLTMGEVLVEVEKMIQDRRCGYVV
                     TPNVDHIVQLETGKELQDVYKNASLILTDGKPLIWISKFYKTPIKEKISGSDIFSLLC
                     KMAAEKGYTMYFLGAAEGVAAKAADNLMKRFPGLNVVGTYSPPYGFEKDEDEMFKIDS
                     QIKRANPDMLIVGLGCPKQEKFMFYNCDRLGVPVSFGLGASLDFEAGNVKRAPKWMSD
                     YGFEWLFRITQDPKRMAKRYLVDDLKIFKLALKYRKK"
     gene            5444..6445
                     /locus_tag="IVO76_001083"
     CDS             5444..6445
                     /locus_tag="IVO76_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAP7429057.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFRENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSTELSYSRTERVTNIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALKGKYADKKKLVDKAIYLERVLCNEAGGWQDQIAAAYGGFNRI
                     NFNSDGYEVLPIIITPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAVLTDKNRDLDDFGRLLDLTWRLKKATGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQNAVRSAMSNLMYIPFEFENGGTQVIHYTPEMYIPK"
     gene            6468..7754
                     /locus_tag="IVO76_001084"
     CDS             6468..7754
                     /locus_tag="IVO76_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAP7429058.1"
                     /translation="MKVVIMAGGKGTRISSVASDIPKPMIKIENKPVLEHEIECLRDQ
                     GFTDIILTVSHLGDIIMNYFGDGSGISPVTGQPFDVKIEYFLEKEPLGNAGALFRLKD
                     NLTEDFLLLNADAMFDVDFNRFVAYHREKGGLVTLFTHPNSHPYDSGLIIADKDGSVD
                     RWLVKEDSRPTYYRNRVNAGLHVISPAVLDVEINTPKIDLDRQLLKPLAGTGKMFCYD
                     SPEYVKDMGTPDRYYAVCEDFKAGRVKGKNLQNKQKAVFLDRDGTINQYVGFLRHIDE
                     FELIKGTEDAVKKINAAGYLAIVITNQPVIARGEVSFEELEEIHNKMETLLGYKGAYL
                     DAVYYCPHHPHKGYEEERPELKFDCECRKPKPGMLIQAATDFNIDLSQSWMIGDGEND
                     IKAGQAAGCKTALVGESEYGQDMTIATLSEFADKIC"
     gene            7788..8441
                     /locus_tag="IVO76_001085"
     CDS             7788..8441
                     /locus_tag="IVO76_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7429059.1"
                     /translation="MIDNRLQKHIDLLIKRYPTLEVIKQDIVDGYLIMEECYLNDGKL
                     LIAGNGGSAADSEHIAGELMKSFKTPRPVSREFADKLIAVDPVKGSELAKNLEYGLMA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRPGDVFLGISTSGNSKNVINATVVARA
                     LGMKVIGLTGAKGGELARVADVCVKAPETETYMIQEYHLPIYHCWCLMLEDKFFGNR"
     gene            8474..9679
                     /locus_tag="IVO76_001086"
     CDS             8474..9679
                     /locus_tag="IVO76_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7429060.1"
                     /translation="MNVLFITTQFPYPLDNGGKIGAYNGISVLSRDYDVTVLSFTEEP
                     QYVEEGVSFFREKFNSVKFENPVIHDVHIRNNPMKLVEALAIGYCLRQPYIVSKFYDS
                     KMLQLIDEKFSNNAYWDIIFIDYLNMQSYGQYIREKYKTQFGKYILKDHNIEYDLVLQ
                     EAESSRGLKKKLLQLEYKRTRKYEEAAVNRADYCFSVCDANTEVLKKINDNSYTMLPT
                     YEMLTGVKKNIEKNTILYLGNLSWKSNMQGLVWFLESIYPNIQQRIPDVKITIVGSGP
                     VGTDFSKYSGVDYRGYVKDISHIYDDQAVLIVPLLEGSGIRIKILEAFNNEIAVVSTL
                     VGCDTIGAKDGSELFIADNEKDFATAVVRLLEDMNLNEKVRKNAKLFLKEKYSLTARQ
                     DEFSAIINS"
     gene            9809..11134
                     /locus_tag="IVO76_001087"
     CDS             9809..11134
                     /locus_tag="IVO76_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429061.1"
                     /translation="MIVAAMSYHLTGNSNVASLIAVVGVVYAIVKACNNEYLAIWVMF
                     VLGSDIWGIVPYINIGSGVLHWSDFNVIVAIFLCLRFIKVKARSRYGIVYSVMLIFIL
                     IACVQSHSIYGQDVHTTLLTARELLVVISFWPIVKIVYSERITKNEFIDLVCKFTRWT
                     MCIYWFEWILVNMGFNITFLQTGARWGTRIYIDPIFIILYFFINLYAVIYKREGYNST
                     NKSIWNCIFSLLTFVIISQGRSGILYVVITSVIMLLISLKPSKIIRFGILGIALIACL
                     VSVTQIREVVISSFVESQSSDTGNIAYRIAEQDYFENKLEGHELFGVGIPNNHDARAV
                     EYSGKTIDFESKKYNPYYLEDLGAFEIRYRFGIIAYALYIVALAISIIDAGFRRDKSV
                     AAFAGMAFMVFMALNFNLLAYITARPFGFMISLILIEISAFNDEFIYIE"
     gene            11151..12281
                     /locus_tag="IVO76_001088"
     CDS             11151..12281
                     /locus_tag="IVO76_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429062.1"
                     /translation="MNLDRLKKIANMISPIIKVINAPLAYFSGKSDYMEKGILWPTVT
                     TYSNNSRKVIQYTTLPKNVDNMAQMQWAGESFEDICIVLQGPVLEDGDFTLNTVKMYN
                     KYYPKVKLVVSTWIGLSEETECKLREAGADVVLCKKPEKTGAYNINLQLTSAYEGVKM
                     AQKLGCSYALKTRTDQRLYANDVLKYFQTLLNIFPSSDCKVVNQRLIFTTYGKSFRYL
                     PFNLCDFLVYGETSELLKLYGIQRDNRASDFGKIIYTEEKEFNKKIFTLLEGKQGVSP
                     YDVFPDFDALYKKYMFAEYYIVYNYFLENIASNESYGDLMDAYYSYLKKYAVVADSEK
                     LLLYWPKYVQYNVKFENELDFYAKLDSKKWMDIYANYTPFSQ"
     gene            12307..13392
                     /locus_tag="IVO76_001089"
     CDS             12307..13392
                     /locus_tag="IVO76_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HAP7429063.1"
                     /translation="MKIGIVTIVNGASFGNRLQNYATQKVLENNGYTVETINNCFDKE
                     STLSIVIKNAIYTLFKFIPHFKKSKSIWKRMYTFYQWDKQYIDYSHRKIDASKKNSFV
                     SIKNDYDYFLVGSDQIWNPKYAANKGFELLSFVPNEKRVSLSTSLGVPSIPSDLEKLY
                     VTEWNKFKSISVRENQAAKIIEDLTGTTAAVHIDPTLMLERSEWEAFQKKPDNIDENY
                     ILSFFLTNPPKDTVDVLDKVAKDLKIGVLTLDASWEYFQDITPNHFVYLISHARMIYT
                     DSFHCTVFSLLFGKPVVVFDRFDSEKMNSRLSTLLNKVNKAHFWVGKKADEAMIRAIA
                     SEPEYDIDKLMVSERECVKAYLKEYLQ"
     gene            13415..14230
                     /locus_tag="IVO76_001090"
     CDS             13415..14230
                     /locus_tag="IVO76_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429064.1"
                     /translation="MEHLSDCFGKMKIVTTSCAREYFKDDLDKSEYSCFVDSRAAMFF
                     AMGQALAKRRNVVVMLADELTSCYTALTECAFQQVDLIVFAFQTVRHEIDLASLKNCG
                     CNLINCSAQDDIDRVVSNTKGLNQTTVIKINMNCESDKDHICEYIDLPDIPVFFHGSL
                     YSPASKKKYEQYGQVSKYLGYLVGGKDAILIISHNEFLNDISVWINYYSFGEIQPKII
                     IFDSPNDYQYKWLCDLECSVAHSTNRFLSEDLALLQNNGISIVLRGDNYDIVS"
     gene            14214..15932
                     /locus_tag="IVO76_001091"
     CDS             14214..15932
                     /locus_tag="IVO76_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylase"
                     /protein_id="HAP7429065.1"
                     /translation="MISSVKNVQILVSLLKQHNIKDIVISPGSRDFQLVHSVETDSFF
                     RTYTVVDERSAAFFANGLSMAIDKPVCVCCTSSTASSNYMAAAKDAFANGIQLVLLTA
                     DRDYRRLYQMEDQMIDQVDMYGAYVRSNVDIPIVKDSDDEWYATRKINEAILELNHGV
                     KGPVQINYQLMDLSAPFVETMPEYRKITRYQEDDLLSSVEIFKQKLLSKKRIMVLCGQ
                     QEENNLSALLEEFFIKYNTIIIKDNYSNIHGEHFIKTVLVTERMSVNAFIDYCPDLVI
                     TIGKHVWSFVKYTLRDKKLKFEHWRISEDGKIVDPFNKLTSVFQCSAEFFMETVTAGV
                     NSNNNLEYYNLWKTRLSKVCYPELVYSNFYAISELVKKIPDSALVHSSILNAARLYDF
                     VSDDKALLSFANLGADGIDGCLPTFLGQAEADKTRPAYLISGDLSFFYAMNSLCGDIP
                     DNAHIFLINNHSGSEFHTNFGKFYNGSLSVDKHIAAGHISNAKDWALENNIKYITADS
                     KESLKSGLDVFINTFDSPVLFEVFTDPDTDTSVLESFYSINKVITPKMYFRKALDIIK
                     KHLHIK"
     gene            15958..16794
                     /locus_tag="IVO76_001092"
     CDS             15958..16794
                     /locus_tag="IVO76_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7429066.1"
                     /translation="MAIKSYIKRLLLYIRHGDPKINLDVSYLSEGAMLAGKTVIITGG
                     AKGLGFAIAKSFIAQGASVIITGRDMDSLNNAKCQLGDMCSIRAFDITDAKMYRTFFE
                     DAYETFGRIDILLNNAGISLHEDGFMSVTEKTWDKQMDTNLKGVYFFTQEFIKFVKEK
                     NQMQANIIMMSSERGLCGDSIPYGLSKAAINSLVVGLSKKYISEGIRVNGIAPGVTAT
                     AMTGISEKGNLYNPATIGYRYFLPEEVAQVALFLASSLSNCISGEIIACDQGQYLSRN
                     WG"
     gene            16799..18232
                     /locus_tag="IVO76_001093"
     CDS             16799..18232
                     /locus_tag="IVO76_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7429067.1"
                     /translation="MGILLKKYNSLSEPAKASIWFTLCNVLQKGILFLTTPIFTRMLS
                     TAEYGKYTIFISWYSIIAIFTTLNLSAGVYNKGLIKYENKYALTSSFLGLSSFISLCA
                     LMLYLPFRTVINGLLDLSTEFVILILIESIFEPAFLLWSVKERFDYKYQKLVLITVLM
                     AVLNPGLGIILILSFGEYAIWRVRAYVVVNVVVGMFIGIGILAKGKKLFDKEYWTYAL
                     RFNLPLIPHYLSMTLLNQVDRLMINSMVGASEAAIYSIAYTISSMMNIITTAINGSFI
                     PFTYKAIKDGVYEKIKSVANQLLVLIFVLCSFTMLFGPELIWVVGGEKYMDAVWIIPP
                     VAASVFFMFLYPLFGNIEFYYEKTNYVMFASCVGSIAKIISNYVSIPRFGYISAGYTT
                     LICYMLFSFAHYMFYKKILRENGIKEDMYNIKNIIFLSVLMIVLTIGMTILYFSTILR
                     YSIIIGLVIVAFLKRTQIMKILKNIKN"
     gene            18249..19910
                     /locus_tag="IVO76_001094"
     CDS             18249..19910
                     /locus_tag="IVO76_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP7429068.1"
                     /translation="MERRHGEMRLLIPSAKIVPEELHHLGKLPAIIYPINQKIVFDYL
                     YDQYKDVCSAIDIACYEKMDKVARRLDKYIKSKTVNIIQLKELGDLGRTIYDSLIGCD
                     EPVIINFADTIINDNIYSLECDSFFYAEDYYSNTWTFFEEKDGDIISVLDKNELKEDD
                     GKKHKLFSGVFQIMDAQYFRECLRKALMSNVVNVNSFYQALQEYSKRYEFLSIKTNNW
                     FDIGHADKYYNSKLEVKAREFNHISIDKDRSILRKISEDVEKFIGEIKWYLKLPAQVE
                     YVRPRIFEYSTSYINPYVSMEYYSYHTVHELFLYSDLTKKQWIDIFNRIRFVCSDFKR
                     YSVSGDNIQKSLKDMYLDKTFQRFNKLRKDPRFTEFFSSDIQINGVRYKSLDQIEALL
                     SVSVPRELFDITQFNIIHGDLCFANIMVDNTFSFIKVIDPRGKFGDFDIYGDYRYELA
                     KLFHSVDGKYDFIIKDLFTIKYDPKKAIIDYIVQDRKRDYDLYEVFYSVFKDEIGSDL
                     KKIELIEALLFLSMIPLHGESLNHQMAMLATGLEILGRVVPDIYC"
     gene            19998..20396
                     /locus_tag="IVO76_001095"
     CDS             19998..20396
                     /locus_tag="IVO76_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP7429069.1"
                     /translation="MFKDYAFVFDIDGTLCPIKKKDEKYEDLVPYANMVEKLRYYKEN
                     GAKITLFTSRNMNSYHGNLGVINANTAKILLAWLDKWEIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLSCTLDQLDEICKKSKLKSE"
     gene            20421..21137
                     /locus_tag="IVO76_001096"
     CDS             20421..21137
                     /locus_tag="IVO76_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429070.1"
                     /translation="MTVIITMAGLGSRFRKVGYTVPKFMIEAKGKTLFDWSLDSLLDY
                     NSHVEKYVFVVRKEDDATVFIKEHCSLYGIENVEVVEIDYMTDGQATTCMLAIPYCAP
                     DNAIMVYNIDTYVEPGEMKYVDISGEGHIPCFHADGDHWSFAKLNEYGDVVEVREKVR
                     ISNNCTLGAYYFSSAKLYASLYEEYYADERHLEKNEKYIAPLYNYMIEKGMKVTISIV
                     DKSKVHVLGTPEELDVFLNE"
     gene            22369..22948
                     /locus_tag="IVO76_001097"
                     /pseudo
     CDS             22369..22948
                     /locus_tag="IVO76_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
     gene            23008..24660
                     /locus_tag="IVO76_001098"
     CDS             23008..24660
                     /locus_tag="IVO76_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7429071.1"
                     /translation="MDHNANYYHWLTNANAEIVEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLSDYLNKTYETPSVVVGYDSRIKSDVFAKVAADIF
                     AANGIQVHLWPELLPVPTVSFAIRYLKASAGVMITASHNPSKYNGYKVYGAGGCQITT
                     EAAAEILAEIEKLDIFADVKMGGDASRIASIQPEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEINEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKNAEGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PTDPVMVKTIVTMDLGERIASNYGVRTVDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLFDKLNEIYAKYGYCLNTLHSF
                     EFEGAAGFTKMQKIMVEFHKGLDMIGGKKVIETLDYSTGLNGLPKSDVLKYLLEGNCS
                     VVVRPSGTEPKLKTYISVSAESKEAAEVMEAVMAAELAEMFK"
     gene            25004..25369
                     /locus_tag="IVO76_001099"
     CDS             25004..25369
                     /locus_tag="IVO76_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAP7429072.1"
                     /translation="MYYFQQSYLPAILNKAISIGDTGVHIFFFCSGFGLYLSYLKKPI
                     TLGEFLQKRFIKIYIPYIIVITVSVLLPYMTQDLNAAKVYLSHVLLFKIFLPVYEESL
                     GPFGLFRQLFSSISYSYQL"
     gene            25457..25900
                     /locus_tag="IVO76_001100"
     CDS             25457..25900
                     /locus_tag="IVO76_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429073.1"
                     /translation="MEAYEFLGAALRNIFGNLLSACLVAEYLVKRKIVRFNNIFVIMA
                     SIVGIGFAGISKIIGGLMESINDIFAFFGYGAIAILMYQVIPFRKIMPRISRYSYDIY
                     LLHILVFSTIHQIIPNLVLAAFVSIPITLVLSYYYSIMLYRLKLK"
BASE COUNT         8345 a         3642 c         5632 g         8413 t
ORIGIN      
        1 attttttaaa agaatgattg atattgtagg aagcattatt ggtttgattt tagcttctcc
       61 tttgatgttc tatgtagctt atcgaatcag aaaagaagag ccaggaagtc cgatattttt
      121 ttgccataca cgtgttggta aaaatggaaa aacattcaaa atgtataaat tccgttcgat
      181 gtgtttagat gctgaagaaa aattggaaga attacttgat caaaatgaaa tagaaggtgc
      241 aatgttcaag atgaaagaag atccccgtgt cactaaaata ggtaaaatta tccgtgccaa
      301 aagcattgat gaattgccac agctatggaa tgttttaaaa ggcgatatga gtctaattgg
      361 tccacggcca cctttggaga gggaagtagc tgagtacacg agatatgatc ttcaacgctt
      421 gtatgtaaaa cctggttgtt caggtttgtg gcaagttagt gatagaaata atgttagttt
      481 ccataaaatg gttgagttgg atttagagta tattagtcat cgaagtttaa tgtacgactt
      541 gaggttgatt ttaaaaacgt tttatgttat tgtcaagccc aatggagcat attagttagg
      601 gatagaatga tggattgtta ataaaaaaga ttacacatgt tcatattatc gtttcaaaag
      661 taataactgt aattatggag ggtttgaatc acttgtagaa aatctaaaaa aagaaaggtg
      721 aatggggcaa ttcagtatca ttattcttat ataaaagaaa atattcgaaa ttttgggatg
      781 ataccactct taaatataat gatctaaatg ctttaatatt ggtatatccg atataggaac
      841 tgacgagcta tttagtatga tacaaaagta tattgatagc cattaataaa ttttccaaaa
      901 aataatgaca gcacaccaat ttttataatt tagcttgttg aatcggtatg ttcatgggac
      961 ggttcaaaaa aagaatcgaa aaaataaacg gtaaaatttt agtgaatcct atgtaataaa
     1021 atttggatag atggaaaaac agcacataag gaggctgagt ttgttgtcat ttctgctcct
     1081 acaaactatg gtagtgaatg tgttattgaa ttagtgatgt ctgttacttc agagacattg
     1141 gggataatta acttcactat acctgtaggg tatacaaaaa atggaagaaa aaatattatc
     1201 aatagatttt atttagttca gaatttttac gtggaagtaa attattgatg ataatcttta
     1261 tccaattaga attattatcg taggatgtga tgatgatagc cgtgaagttg ttcaagtatt
     1321 taccaatgtc tacaacgcag aaaattagtt tgggtttttc ggattggaaa atttcaatgt
     1381 ataaaatcat aaatttgctg taaatagaca gtttggggta gtaatagccc aataaattgt
     1441 tcgataaact catctttggg gtgagaatag ctcaatcatt gaatttcaaa cctgcctttg
     1501 gggtgggatt agaggtcaat atgtacctac ataatacttt ttggaaaatg atggtgttga
     1561 gaatgtaagt acatttcaat gtgaaattct aatatagccc caaacgaggt caataaaaat
     1621 gacctaaact cgtatttctc ccctaaacga ggaggagata gatatgaaaa ttgaatgtgc
     1681 cggcgttgga gttggctgct tttagtagga atgaacatgg ttcgatgaga actgtttcag
     1741 atagagtggt cgatgatcac aggcctttaa gtcgtttaca gatgatgaga cttgtaaaaa
     1801 tagagggacg aatggaaaaa gatgattggt atagaagaaa agaaaggtgt tcagaaagac
     1861 gtaacaattg atagggaaga gaatcttagg ggacatagat tctattggat tggacgcaga
     1921 gcacaagata tcttcttctc ggctctagct ttgattgcat tgagccctgt gatgctaatt
     1981 acagcgctcg ccattgtgat tgacgatccc ggtggcagcc cgatttttgt gcaggataga
     2041 gttggaagaa atggaaagct gttcaagttt tataaattcc gatctatgtg tgtagatgct
     2101 gaagcaaaat tagaagcctt gttggcacaa aacgagatgg atggtccggt cttcaagatt
     2161 aaagatgacc caagaatcac gagagttggt aaatttattc gaaagacaag tatcgatgag
     2221 ttacctcagc tctggaacat tcttaaaggt gatatgagta ttattggacc cagacctgct
     2281 ttaccgagag aggtggaaca atacggtgac tacgaaaggc agcggttgtt cgtacagcct
     2341 ggtcttagtt gctattggca gattgctcca catcgtaatg aactgtcctt tgaagaatgg
     2401 gtggaacttg atgtgaagta catcaatgaa agaagcttta tcacggactg gaagattatc
     2461 ttcttgactt tacaggctgt attatttgga tatggtgaat gattatatat agtattagga
     2521 gttgaaatat gtgaatatta ttttattatc gggtggtagc ggtaggcggt tatggccgct
     2581 gtccaacgat attcgtagca aacaattcat caaactgttt aaggatgaaa atggcgaata
     2641 tgaatctatg attcaaagag tttatcgcca gattacaact gttgacccag atgcaaaggt
     2701 cactattgca acaagtaagt ctcaggctag tgcaatccat aatcagttgg gagataaggc
     2761 ttcgatttgt gtagaacctt gtcgtagaga tactttccct gcaattttgt tagctgcagc
     2821 atatcttcac gatgaacttg gcgtaggtga tgatgaaagt gttgcggttt gtcctgttga
     2881 tccttatgta gataattcct attacgaagg cgttgaagcc cttgagaagt tggtgcagca
     2941 gaatacggct aatttgactt tgatgggtat tgcacctaca tatcctagtg aaaagtacgg
     3001 ttatatcatc cctgaaagtt ctaacgaagt aagtactgtt aaggagttta aggaaaaacc
     3061 tgacaaagca acggcagaga aatatttagc tcagaatgct gtttggaatg ccggtgtatt
     3121 tgcgttcaaa cttggttacc tgttggaaaa agctcacgca atggtggatt ttgaagatta
     3181 tcgtgatttg tattccaagt atgagacttt aaccaagatc agctttgact atgctgtggt
     3241 cgaaaaagaa acgagcattc aagtgatgcg ttataacggg gattggaaag atgtcggaac
     3301 ttggaacatg atggctgaag ttatggctga taatattaag gggaaagctg taatggatga
     3361 tacctgtgtt aatactcaga ttgtgaatga gttgaatatt cctattcttg ctatgggctg
     3421 caaagatatg gtaattgcag caagtggcga tggaattctg atttctgata aagagcgcag
     3481 tggctatatg aagccgtatg ttgagaaaat cagtacggaa gtaatgtttg cagaaaaatc
     3541 ctggggtaca tataccgtta tggacgttca ggaaggctcc atgactgtta aagtttctat
     3601 gcaggccgga cagaagttga gctaccacta ccacaattct cgcgatgaag tttggactgt
     3661 tgtagcagga cagggtaaag cgattgtaga tggtatgaag cagattcttc gtgcgggtga
     3721 tgtggtgact attgccgcag gatgtaaaca tacaatcgaa gccgtgacaa atctggatat
     3781 tatcgaagtt cagattggtg aaactattag cgtcgctgac aagtataaga gttctttgaa
     3841 ctaaaagttc gcgaggtagt tattatggag atactaaaat ttcaacccgt gggtaaagat
     3901 tatctttggg gtggagaacg gtttaagacg gaatttggaa aaaattgact taacaccatt
     3961 aagcttatac gtgggaatgc ttagttcatc tagacgataa ctacgtacgg aaatatcaaa
     4021 tatgaaggac agaatggtaa aaccgagttg tggtatgtat tggaagctac accggggggt
     4081 gatttatgct tttgagtacg atgtaacaga agaaatgtta cgaaatgctg tgatggacag
     4141 atcacttggg aaacatttat ataaagaaga agtacacaag ggagatcttt tttatgtcag
     4201 ttcatggtat tggcgctgga gtattagtgg ctgaaataca ggaaagctca aatgttacct
     4261 atcgagttta cgattatgat cgagtagata agaatgataa taagtgagaa ttgtactttg
     4321 aaaaagcgat tcaggtaatg aatatggaag ccactttgaa attggagaaa agaaataagc
     4381 tcattcattt ttatcctggt ggctctcgtg aaatactgtg tagatgtaaa tactgtgagc
     4441 ccgaaagaat ccaagtaaag acggggtttt cgttctcggt aaaagaggaa tcttttcaag
     4501 ttcttttatg tatatcgggg gacggacaag ttgagtcggc aaatggtgtt atgaaactca
     4561 ttagatttgt aaaaaagata ttgtatgttt ttgccaacga atattggaag atgttaagtg
     4621 ttgagcgatg cggacttact gaaaattaga tggggataga gggaggatat ctaatgtcac
     4681 gaatgagatt tatgaacaca gaaattgaca atcttacaat gggtgaagta ttggtagagg
     4741 ttgaaaaaat gattcaagat aggagatgcg gatatgttgt aactccgaat gtggatcaca
     4801 ttgttcagtt ggaaacagga aaagagttgc aagatgttta taaaaacgca tctttaattt
     4861 tgacggatgg taaaccattg atttggattt caaagtttta taagacacca ataaaagaaa
     4921 aaatatcggg atccgatata ttttctttac tgtgcaagat ggctgcagaa aagggataca
     4981 caatgtattt tttaggtgcg gcagagggcg ttgcggctaa agcggcagat aacctgatga
     5041 aaagatttcc gggattaaat gttgtaggaa catactctcc tccttatggg ttcgaaaaag
     5101 acgaagatga aatgtttaaa attgattccc aaattaaacg agcaaatcca gatatgctta
     5161 ttgtgggttt gggttgccct aaacaggaaa aatttatgtt ttataactgt gatagattgg
     5221 gagtacctgt gtcttttggt ctaggagcta gtttggactt tgaggcaggg aatgttaaaa
     5281 gagcaccaaa atggatgtca gattatggat ttgagtggct gtttagaatt actcaggacc
     5341 ctaaaagaat ggcaaaacga tatttggttg atgatttaaa aatctttaaa ttagcactaa
     5401 aatatcggaa aaaataaact atattcaatg aggacaatga agtatgatta ttacaaaaac
     5461 accttttcgc atgtcttttt ttggcggcgg aacggatatg gaagattatt ttcgagaaaa
     5521 tggcggtgca gtgctttcca ctacttttga taagtactgt tatgtgaatg tgcgtcattt
     5581 gcctagattt tttgattaca gcacagagtt atcatattct cgtactgaga gagtaacaaa
     5641 tattgaagat atcgagcatc ctgcaattcg taatgcgatg aaaatgctag atatgcatga
     5701 aattcgcctt acatatgaag cagatcttcc tgcacgttct ggtctgggta catctagttc
     5761 ttttgccgta ggtatgttga atgctttcta tgctttgaag ggcaagtacg ctgacaagaa
     5821 gaaactcgtt gataaagcaa tttatcttga aagagtcctt tgtaatgaag caggcggatg
     5881 gcaagatcag attgcagcag cttatggtgg ctttaaccga atcaatttca actcagatgg
     5941 ctatgaagta ttgcctatta ttattacgcc tgagcgtaaa aagcagctta acaacaactt
     6001 gatgatgttc tttactggat tcaccagatt ttcctctgat gttcagaagg caaataatgt
     6061 cagcggcaca gaggacaaaa gagtaagact gaaaaaaatg tatgagcttg tggatgaagc
     6121 agaagcggtt ctgactgata agaacagaga tctagatgat ttcggcagac tgcttgatct
     6181 cacttggaga ttgaaaaagg ctaccggtgg tgcgattagc actggaagta tcgatgaact
     6241 ttatgaaaag ggcatggctg ctggtgcatt gggcggtaaa cttctcggag caggtggcgg
     6301 cggtttcttg gttttttatg ttcagcctga aaaacaaaat gctgtgcgtt cggcaatgag
     6361 taatttgatg tatattccgt ttgaatttga aaacggtgga acacaggtta ttcattacac
     6421 accggaaatg tatattccaa aataaagaaa tcagcgagga taagaatatg aaagttgtta
     6481 ttatggcagg cggtaaggga actcgcatct catcggttgc aagcgatatt ccaaagccca
     6541 tgattaaaat tgaaaacaaa ccggtattgg agcacgaaat cgaatgtctg agagaccagg
     6601 gattcacaga tattattctt accgttagcc atcttggcga tatcattatg aactattttg
     6661 gcgatggttc tggaatttca ccagttactg gtcagccctt tgacgttaaa attgaatact
     6721 ttttggagaa agagccatta ggaaatgccg gtgcattgtt tcgtttaaag gataatttga
     6781 ccgaagactt cctcctgctg aatgcagatg cgatgtttga cgttgatttc aaccgatttg
     6841 tagcatatca ccgtgaaaag ggtggcttgg tgacattgtt tactcaccct aacagtcatc
     6901 catatgacag cggtctaatc attgcagata aggatggctc tgtagacaga tggcttgtaa
     6961 aggaagattc ccgtccaact tattaccgta acagagtaaa tgcgggactc catgtgatta
     7021 gtcctgctgt tcttgatgtc gagataaaca cacctaagat tgacttggac cgtcagttat
     7081 taaagccgtt ggcaggaacc ggaaaaatgt tctgctatga tagtccagaa tacgtaaaag
     7141 acatgggtac ccctgatcgt tattatgctg tctgtgaaga ttttaaggct ggaagagtga
     7201 agggcaaaaa tcttcagaat aagcagaaag ctgttttcct tgatcgtgat ggaacgatta
     7261 atcaatatgt tggctttctg cgtcatattg atgagttcga actaatcaag ggtactgaag
     7321 atgcagtgaa aaagatcaat gccgctggat atctggctat tgtaataacg aatcagccag
     7381 taattgcaag aggtgaagtt tcttttgaag aattggaaga gatccataac aaaatggaga
     7441 cacttcttgg atataagggc gcttatttgg atgcagttta ttattgcccg catcatccac
     7501 ataagggata tgaggaagag cgcccagagc taaaattcga ttgtgaatgc agaaagccaa
     7561 agcccggaat gttaattcag gcagctacgg acttcaacat agatttgagt cagtcttgga
     7621 tgattggcga tggcgagaat gatattaaag cgggccaagc cgccggctgt aagactgcat
     7681 tggttggcga gagtgagtat ggacaggata tgaccatagc aaccctgagt gaatttgcag
     7741 ataagatctg ctaagggatg ttttatcacg aaaggagagt ataaacaatg atagacaata
     7801 gattacagaa acacattgat ttgctgatta aacgctatcc cactcttgaa gtaatcaagc
     7861 aggatattgt tgacggatac ctgattatgg aggaatgcta tttaaatgat ggcaagctgc
     7921 tgatagcagg aaatggcgga tctgctgctg attccgagca tattgcgggt gagctgatga
     7981 aaagctttaa gacgcctcgc cctgttagta gggaatttgc agataaattg attgcggttg
     8041 atcctgtaaa gggttctgaa ttagctaaga atcttgaata tggtttgatg gcgattcctc
     8101 tcgttgctca tgaagcgctt tccactgcgt atattaatga tgttgatggc ctaggtgttt
     8161 tcgcacagca gttgtttgga tttggaagac ctggcgatgt attccttggt atttcgacat
     8221 ccggcaatag caaaaatgtg ataaatgcaa ctgttgttgc tagagctctt ggtatgaaag
     8281 taattggctt aactggagcc aaaggtggag aacttgcaag ggtagcagat gtttgtgtta
     8341 aagctcctga aacagaaaca tatatgattc aggagtacca tttgccaatt taccactgtt
     8401 ggtgtctaat gttggaagat aaattttttg ggaatagata aaatatctaa gcccatattg
     8461 aaaggataaa attatgaatg tactatttat aacaactcaa tttccatatc ctctagataa
     8521 tggcggaaaa attggtgcgt acaacggtat cagtgtttta tctagagact acgatgtgac
     8581 ggtattgtct ttcaccgaag aaccgcagta tgttgaagaa ggtgtcagtt tttttcgaga
     8641 gaaatttaat agtgtaaaat ttgaaaatcc tgtgatacat gatgtacaca taagaaataa
     8701 tccaatgaaa ttggtagaag ctttagcaat agggtattgt ctacgccaac cttatatagt
     8761 ttcaaagttt tatgattcaa aaatgctgca gttaatagat gaaaaatttt ctaacaatgc
     8821 atattgggat atcattttta ttgactatct gaatatgcaa agttatggtc aatacatacg
     8881 ggagaagtat aaaacacaat ttggtaagta tatcttaaaa gaccacaaca tagaatatga
     8941 tttagtccta caggaagctg aatcaagtcg aggtttaaaa aagaagttgc ttcagttaga
     9001 gtataagcgt actcgaaagt atgaagaggc cgcagtaaat agggccgatt attgctttag
     9061 tgtgtgtgat gcaaatacgg aagtactgaa aaagattaat gacaacagtt atacgatgct
     9121 tccaacttat gaaatgctta caggggtgaa aaaaaatata gagaagaaca cgatcctata
     9181 tttgggtaac ctttcttgga aatccaatat gcaaggatta gtttggtttt tagagtcaat
     9241 ttatccaaat attcaacaaa gaattccgga tgtaaaaatc acaattgtcg gtagcgggcc
     9301 agttggaaca gattttagta aatattcagg cgtggattat cgtgggtatg taaaagatat
     9361 ttcacacatt tatgacgatc aagcggtgct aattgtacct ttgttagaag gtagtggtat
     9421 aagaataaaa atattagaag cgttcaataa tgaaattgca gttgtatcta cattagttgg
     9481 gtgtgatacg attggcgcaa aagatggaag tgagttattt atagcagata acgaaaaaga
     9541 ttttgcaacg gctgtagtta gactgcttga agacatgaat ttgaatgaaa aagtaagaaa
     9601 gaatgcaaaa ttattcttaa aagaaaaata ctccttaacg gctagacaag atgagtttag
     9661 tgcgataatt aacagctaat atatatataa gcttatctga tgatgattta taggaggaaa
     9721 tatatggaga tggctgagag aaaggaaaag aaacttggaa ataagaatat catattattt
     9781 tagaaaactc ctgttatttt atgtttgtgt gattgttgcg gctatgagtt accaccttac
     9841 aggcaattcc aatgtagcgt cattaattgc tgtagtagga gtagtttatg ctattgtaaa
     9901 ggcgtgtaat aacgagtatc tggctatatg ggttatgttt gtattaggtt ctgatatatg
     9961 ggggatagtg ccatatatta acattggtag tggtgtgttg cattggtcag attttaatgt
    10021 tatagtagcg atatttctct gcttgagatt catcaaagtt aaagctagga gtagatacgg
    10081 gattgtctat agtgtaatgc ttatatttat tttgattgca tgtgtgcaat cgcattcaat
    10141 ctatggacaa gatgtacata caacgctctt aacagctaga gaattgctag ttgtaatatc
    10201 cttttggccg atagtaaaaa tagtatattc agaaagaata actaaaaatg aattcattga
    10261 tttagtgtgt aaattcacaa gatggacaat gtgtatatat tggtttgaat ggattcttgt
    10321 taatatggga ttcaatataa cctttttaca gacaggtgca cgctggggta ctagaatcta
    10381 tatagatccg atatttatca ttttatattt ctttataaat ctatatgcgg ttatatataa
    10441 aagagagggc tataatagta caaataaaag tatatggaat tgcattttta gtttactgac
    10501 atttgttata atctcgcaag gcagaagtgg tatattatat gtagtaataa cctcggttat
    10561 tatgctttta atttcgctta aaccatccaa gattattaga tttggtatat taggaattgc
    10621 cttaattgca tgtctagtgt ctgtgaccca aattagagag gtcgttatat cttcgtttgt
    10681 agagtctcag agcagtgaca caggtaatat cgcatataga attgctgaac aagattattt
    10741 tgaaaataaa ttagaaggtc atgaactatt tggagttggt ataccaaaca atcatgatgc
    10801 cagagctgta gaatattcag gcaaaacaat agattttgag tctaaaaaat ataatcccta
    10861 ttatcttgag gacttaggcg cttttgaaat tcgttataga tttggtataa ttgcatatgc
    10921 gttatatata gttgctttag ctataagtat tatagatgcc ggatttcgaa gagacaaatc
    10981 ggtcgccgca tttgcgggta tggcattcat ggtgtttatg gcgcttaatt ttaatttgct
    11041 agcgtatatt acagcaagac cgttcggatt tatgatttct ttaattttga tagaaatatc
    11101 ggcattcaat gatgaattca tatatattga ataggtaagg gagagcacag atgaatttag
    11161 atcgattaaa gaaaatagca aatatgattt caccaatcat aaaagtgata aatgcgccgt
    11221 tagcgtattt tagtgggaaa tcagattata tggagaaggg aatattgtgg cctactgtaa
    11281 caacctactc caataacagt agaaaagtga ttcaatatac aactttacct aaaaacgtag
    11341 acaacatggc tcaaatgcaa tgggctgggg aaagttttga ggacatatgt atagttcttc
    11401 aaggtccagt attagaagac ggtgatttta cactaaatac cgttaaaatg tataataaat
    11461 actatcctaa agtaaaactt gtagtgtcta cttggatagg attatctgaa gagacagaat
    11521 gcaagttgcg tgaagctggt gcagatgttg tattatgcaa gaaacctgaa aaaacaggtg
    11581 cgtacaatat taatctacaa cttacttctg catacgaagg cgtaaaaatg gcgcaaaaac
    11641 tggggtgtag ttatgcgctg aaaacgcgaa ctgatcaaag actatatgca aatgatgtac
    11701 taaagtattt ccaaacatta ttgaatattt ttccatcttc ggactgtaaa gtagtgaatc
    11761 agcgattaat atttacgact tatggaaaat catttagata tcttccgttt aatttatgtg
    11821 attttttggt atacggagaa actagtgaat tattaaaatt atatggtatt caaagagata
    11881 accgagcatc agactttggg aaaattattt atactgagga aaaagagttt aataaaaaga
    11941 tatttacact actagaagga aaacaaggtg tatctccata tgatgttttt ccagattttg
    12001 atgcactata taaaaaatat atgtttgcag aatactatat tgtgtataat tatttcctgg
    12061 aaaatattgc aagtaatgaa tcttatggtg acttaatgga tgcctattat agttatttaa
    12121 agaaatatgc agttgttgct gattcagaaa aattgttact atattggccg aaatatgttc
    12181 aatataatgt taagttcgaa aatgaacttg atttctatgc gaagttggat tcaaaaaaat
    12241 ggatggatat ttatgctaat tatacacctt ttagtcagtg attatatgga aaggatgagg
    12301 tgaataatga aaatcggaat agtgactatt gttaatgggg caagttttgg taacagatta
    12361 caaaattatg cgactcaaaa agtgttggaa aataacggct atacagttga gacaattaat
    12421 aattgttttg acaaagaaag taccctatcc attgttatca aaaacgctat ttatacatta
    12481 tttaagttta taccgcattt caaaaagtca aaaagtatct ggaaaagaat gtatacattt
    12541 tatcaatggg acaagcagta tatagattat tctcatcgta aaattgatgc atctaaaaaa
    12601 aacagctttg tgagtataaa gaatgattat gactatttct tggttggtag cgatcaaatt
    12661 tggaatccaa aatatgcagc caataaggga tttgaacttt tatcatttgt tccaaatgaa
    12721 aaaagagttt cattatcaac cagtttggga gttccatcta taccaagtga tttagaaaaa
    12781 ttatatgtga cagagtggaa taagtttaaa tcaatatcgg tacgtgaaaa tcaagcggca
    12841 aaaataattg aggacttgac aggcacaact gcggctgttc acattgaccc aactttaatg
    12901 ttggagcgtt cggaatggga agcctttcaa aaaaaaccag acaatattga tgaaaattac
    12961 atcttgtcgt ttttcttaac taatccgcct aaggatactg ttgatgtatt agataaggtc
    13021 gctaaagatt tgaaaatagg tgttctcacc ttagatgcgt cttgggaata ttttcaagat
    13081 attactccga atcattttgt ttatttgatt tcacatgcaa gaatgattta tacagattca
    13141 ttccactgca cagttttttc attgctattt ggaaaaccgg ttgtggtatt tgaccgtttt
    13201 gatagtgaaa aaatgaattc taggttaagt acattgttaa ataaagtaaa taaagcacat
    13261 ttttgggtag gaaaaaaagc tgatgaagca atgattcgtg caatagcatc tgaacctgaa
    13321 tatgacatag ataagctgat ggtatcagag agggaatgtg taaaagctta tcttaaagag
    13381 tatttacagt aaaattttga aatgggatga aaatatggag catttaagtg attgttttgg
    13441 taaaatgaag attgttacga cttcatgcgc aagagaatat tttaaggatg atttggataa
    13501 atcagagtat tcatgttttg ttgattctcg agcagctatg tttttcgcaa tgggacaagc
    13561 tttagctaaa agaaggaatg tagttgtaat gcttgctgat gaattgacat cgtgttatac
    13621 ggcactaaca gaatgtgcat ttcaacaagt tgatttaatt gtgtttgctt ttcagacagt
    13681 cagacatgaa attgatttag catctttgaa gaattgtggt tgcaatctaa ttaactgtag
    13741 cgcccaagac gatattgata gagttgtgtc caacactaag gggttaaatc agacaacagt
    13801 aattaaaatc aatatgaatt gtgagtcgga taaagatcat atatgtgagt atatagacct
    13861 gccagacata cctgtttttt tccacggtag cttatactca cctgcaagta aaaagaaata
    13921 tgagcagtat ggacaggtat cgaagtattt aggctatctc gtggggggaa aagatgcaat
    13981 actcataatt tctcacaatg agtttttaaa cgatatcagt gtatggatta attattattc
    14041 ttttggagaa attcaaccca aaattattat atttgacagt ccgaatgact atcaatataa
    14101 atggctgtgt gacttagaat gcagtgtagc tcattcaacc aaccgatttt tgtctgagga
    14161 tttagcctta ttgcaaaata acggaatttc cattgtgtta agaggtgata attatgatat
    14221 cgtcagttaa aaatgttcaa attttagtat ctttattaaa acagcataat attaaagata
    14281 tagttatctc gccaggtagt agagattttc aattggtgca tagtgtagaa acagacagtt
    14341 tttttagaac ctatacggtt gtagatgagc gcagtgctgc cttttttgct aatggacttt
    14401 ctatggcgat tgataagccc gtgtgtgtat gttgtacttc ttcaactgcc tcttcaaatt
    14461 atatggcggc agctaaagat gcttttgcta atggcatcca acttgtactt cttactgctg
    14521 atagagatta ccgccgctta tatcagatgg aagatcagat gattgatcag gtagatatgt
    14581 atggggctta tgtgcgttct aacgttgata ttcctattgt taaggattct gatgatgaat
    14641 ggtatgctac cagaaaaata aatgaagcaa tattggagtt aaatcatggc gttaagggtc
    14701 ccgtgcagat taattatcaa ttaatggatt taagtgcgcc gtttgttgag accatgccag
    14761 agtatcgtaa aattacaaga tatcaagagg atgatttact gtcatcagta gagattttta
    14821 aacagaaact attatcaaaa aagagaataa tggttttatg tggacagcag gaagaaaata
    14881 atttaagtgc cttattggaa gagttcttta ttaaatataa tacaattata atcaaggata
    14941 actattctaa tatacacggt gaacacttta ttaagactgt tctagtaaca gaaagaatga
    15001 gtgtaaatgc atttattgat tattgcccag atttggtaat aactattggt aaacatgtat
    15061 ggtcttttgt gaaatatact cttagagata aaaagcttaa atttgagcat tggcggatat
    15121 ctgaggatgg taagattgtt gatccgttca acaagctaac atctgtcttt cagtgctcag
    15181 cggaattttt tatggagact gtcactgctg gtgtgaatag caataataat cttgagtatt
    15241 ataatctatg gaagacaaga ttatcaaaag tttgctatcc agagttagtc tatagcaatt
    15301 tttatgcaat ttcagaacta gtaaaaaaaa ttccagatag tgcattagtt cattctagta
    15361 ttttaaatgc ggctagattg tatgactttg tatctgatga taaagcatta ctcagttttg
    15421 ctaatttagg ggctgatggt attgatggtt gtttaccaac atttttaggt caggcagaag
    15481 cagataaaac tcgacctgcg tatttaattt caggagactt gagtttcttt tatgctatga
    15541 atagtttatg tggtgacatt ccagataatg cacatatttt ccttattaac aatcattctg
    15601 gaagtgaatt ccatacaaat tttggtaagt tttacaacgg atcattaagt gttgataagc
    15661 atatagctgc aggacatatt tcaaacgcaa aggattgggc attagaaaat aatataaaat
    15721 acattactgc ggattctaag gaaagtctca aatcggggtt ggatgtattt attaatacat
    15781 ttgacagtcc tgttttattt gaggtcttta cagatccgga tacggatacg tctgttctgg
    15841 aatcttttta tagtataaat aaggtaatca caccaaaaat gtattttaga aaggcgttag
    15901 atattataaa aaagcattta cacattaagt agatgtttat tgcgaggtga aacaaatatg
    15961 gcaataaaat cttatattaa acggttattg ctttatatcc gtcatggaga tcccaaaata
    16021 aatttagatg tgagttattt atctgaaggt gcaatgcttg ctggtaagac tgtgataatt
    16081 acgggtggtg caaaaggact tggttttgca attgcaaaat catttatagc tcaaggcgca
    16141 agcgttatta ttacaggaag agacatggat tcgttgaata atgctaaatg tcagcttgga
    16201 gatatgtgtt ctatcagggc gtttgatata actgatgcta aaatgtatcg aacgtttttt
    16261 gaagatgcat acgagacctt tggacgtatt gatatactgc ttaataatgc tgggatctcg
    16321 ttacacgaag atggatttat gagtgttact gagaaaactt gggataagca aatggataca
    16381 aatttgaaag gtgtttattt ctttacacag gaatttatta agtttgttaa agaaaagaat
    16441 caaatgcaag caaatattat tatgatgtcc tcagaaagag gcttgtgtgg tgatagtatt
    16501 ccgtatggat tatcaaaggc tgcaattaac tctttagttg taggcttgtc taagaaatat
    16561 atttctgagg gaattagggt taatggtatt gctcctggag taactgcaac cgctatgact
    16621 ggtatttctg aaaagggtaa tttgtataat cctgcaacga tcggttatag atattttctc
    16681 ccagaagaag ttgctcaagt tgctttgttt ttggcaagta gtctttcaaa ctgtatcagt
    16741 ggtgaaatca tagcctgtga ccaggggcaa tatttaagtc gaaattgggg gtaataactt
    16801 gggaatatta ttaaaaaaat ataatagcct tagtgagcct gcaaaagcat ctatttggtt
    16861 tacactttgt aatgtgttgc aaaaaggaat tctatttttg actacaccta tttttacaag
    16921 aatgctttca actgcagaat atggtaagta tacgattttt atatcgtggt attctattat
    16981 agcaattttc acaacgttga atttatctgc aggtgtctat aataaaggac taattaaata
    17041 cgagaataaa tatgcattaa cctcgtcatt tttaggatta tcgtcattta tttccctttg
    17101 tgcattgatg ctataccttc catttagaac ggttataaat gggctactgg atttatcgac
    17161 ggagtttgtc atattgattc ttattgaatc aatatttgag ccggcttttt tgctttggtc
    17221 agttaaggaa cgatttgatt acaagtatca aaaactagta ctcatcacag ttcttatggc
    17281 tgttttaaat ccaggtctcg gtattatatt aatcttatca tttggggagt atgctatttg
    17341 gagagtacga gcttatgtcg tggtaaatgt tgtagtgggg atgtttattg gcataggaat
    17401 actggctaag ggtaagaaat tatttgataa agagtactgg acatatgcgc tcagatttaa
    17461 tttgccatta ataccacatt atttatcaat gacattgctg aatcaggttg atcgccttat
    17521 gataaatagt atggttggag ctagcgaggc tgcgatttat agtatcgctt acactatatc
    17581 tagtatgatg aatatcataa caacagctat aaacggttct tttattcctt ttacatacaa
    17641 agcaattaaa gatggcgtgt atgaaaaaat taaatccgtt gctaatcaac tattagtgtt
    17701 gatttttgtc ttgtgttcgt ttactatgct gttcgggcca gaattgattt gggttgtcgg
    17761 aggagaaaaa tatatggatg ctgtttggat tattcctcca gttgcggcat cagtattttt
    17821 catgttttta tatccgcttt ttggcaatat tgaattttac tatgaaaaga caaactacgt
    17881 aatgtttgca tcttgtgtgg gctctatcgc aaaaattatt tcaaactatg ttagtattcc
    17941 ccgttttgga tatatttcgg ctggttacac gacattgatt tgctatatgt tgttttcctt
    18001 tgcccactac atgttttata aaaaaattct tagagaaaat ggtataaaag aagatatgta
    18061 taatattaaa aatattatat ttctttctgt cttaatgatt gtgttaacta ttggtatgac
    18121 aatattatac ttttctacaa ttctaagata tagtattatt attggattag ttattgtggc
    18181 ttttcttaaa aggacgcaaa taatgaaaat ccttaaaaat ataaaaaatt aacaagcaaa
    18241 tcttgggaat ggaacggagg cacggtgaaa tgagattatt gattccatct gctaagattg
    18301 taccggaaga acttcaccat cttggaaaac ttcctgcaat tatctatccg attaatcaaa
    18361 aaattgtatt tgattattta tatgatcaat ataaagatgt atgttctgct atcgatattg
    18421 cctgttacga aaagatggat aaggtagcaa gacgattaga taagtatatt aaatctaaaa
    18481 cagtaaatat tattcagctt aaagagttag gggatcttgg aagaactata tatgattctc
    18541 taataggatg tgacgagcct gttattatta attttgctga taccattatc aatgataata
    18601 tatattcctt ggaatgtgac agctttttct atgctgaaga ctattatagt aatacttgga
    18661 cattctttga agaaaaagac ggagatatta tttctgtttt agataagaac gagttaaaag
    18721 aagatgatgg aaagaagcac aaactgtttt ctggtgtatt tcaaattatg gatgcccagt
    18781 attttagaga gtgtttgcgt aaagctttga tgtcaaatgt agtaaatgtg aatagttttt
    18841 atcaagcttt acaagagtat agcaaacgat atgaatttct ctctattaaa acaaataatt
    18901 ggtttgacat tggacatgca gataaatatt ataattccaa attggaagtt aaggcaagag
    18961 aatttaacca catttcaatt gataaagatc gtagcatcct gagaaaaatt agtgaagatg
    19021 ttgaaaaatt tatcggagaa attaagtggt atcttaagtt gccagcacag gtagaatatg
    19081 tgagacctag aatattcgaa tattctactt cgtatattaa tccatatgta agtatggaat
    19141 actattcgta tcatactgtt cacgagttat ttctctatag tgacttaaca aaaaaacaat
    19201 ggatagatat ctttaataga atacgatttg tatgctcaga ctttaagaga tactctgttt
    19261 caggggataa tattcaaaag tcattgaaag atatgtatct cgataaaaca tttcagagat
    19321 tcaataagct cagaaaggac ccccggttca cagaattctt ttcttctgat attcaaataa
    19381 atggagtcag atataaatct ttggaccaga tagaagcttt attgagcgta agtgttccaa
    19441 gagaattgtt tgacattacg caatttaata ttattcatgg tgacttatgc tttgccaata
    19501 ttatggtaga taacacattt tcgttcatta aagtcataga tccgcgaggt aagtttggag
    19561 attttgatat ttatggtgat tatagatatg aactggcaaa attgtttcat agtgttgatg
    19621 gaaaatatga ctttataatt aaggacttgt ttacaataaa atatgatcca aagaaggcaa
    19681 tcatagacta tattgttcaa gatcgtaaac gagattatga tttatatgaa gtattttact
    19741 ctgtcttcaa agatgaaata ggttctgatt tgaaaaaaat tgaacttatt gaggctttgc
    19801 tgtttttaag tatgattcct ttgcatggag aaagcttaaa ccatcagatg gctatgttag
    19861 caactggttt ggaaatttta ggacgagttg tcccggatat ttattgttaa tgctttgagc
    19921 tattgttttt ctatttgatc agtgtaaagc cgggcatata aattgcctgg ctaaaataaa
    19981 ggagatgtct tagttatatg tttaaagatt acgcatttgt ttttgatatt gatggaactt
    20041 tgtgccccat caaaaagaaa gatgagaaat atgaagattt agttccgtat gctaatatgg
    20101 tggagaagtt gcgatactat aaggagaacg gagcaaagat tacactgttc acatctcgca
    20161 atatgaattc ttatcatggt aatttaggtg tgatcaatgc aaatactgca aaaatcttgt
    20221 tggcttggct agataagtgg gagattccgt atgatgaaat tctttatgga aagccttggc
    20281 caggccataa agggttttat gttgatgatc gcacagttcg tccggatgaa tttttatcgt
    20341 gtaccctgga tcagctcgat gagatttgca aaaaatccaa attgaaatct gagtaaagga
    20401 gactttataa agtgaataag atgacggtta ttattacaat ggctggtctt ggcagtcgct
    20461 ttcgtaaagt tggctacact gtgcccaaat ttatgattga agcaaaaggt aaaaccttat
    20521 ttgattggtc cttagacagc ttgcttgatt ataattcaca tgtagaaaag tatgtttttg
    20581 ttgtgcgtaa ggaagatgat gctactgttt ttatcaaaga acattgcagt ctgtatggaa
    20641 ttgaaaatgt cgaggtagtt gaaattgact atatgactga tggacaagcc actacatgta
    20701 tgcttgcaat tccttattgc gcacctgata atgcaattat ggtttataac atagatacat
    20761 atgttgagcc aggtgagatg aaatacgttg atatttctgg agaaggacat attccgtgtt
    20821 tccatgcgga cggtgatcat tggagttttg ccaagttgaa cgaatatggt gatgtagtag
    20881 aagtaagaga aaaagttaga atttctaaca attgtacttt aggtgcatat tatttttctt
    20941 ctgcaaaatt atatgcatca ctctacgaag aatattatgc agatgaaagg catctggaaa
    21001 agaatgagaa atatattgcg ccgctatata attatatgat agagaagggc atgaaggtaa
    21061 ccattagtat agtagataaa tcaaaagttc acgtactcgg tactccagaa gagctcgatg
    21121 tgttcttaaa tgaataggga aaatataatc atagaaaatg ttaattataa agctgcgtgt
    21181 gtgggggagt actattcgat agatattcta aaaatactaa tggctatttt agttgttgtt
    21241 cgtcatttag gtcaaaacta tttagctcca gattaagcat ttagtattgg tattgttgca
    21301 ggactgagta cggttgccgt tcctactttt ttcatcatca gttccttttt gttgtgtagt
    21361 ggtgaagtta catccgctaa attatctaaa caaatctaca gagtacttaa aatttacatt
    21421 atttggtcta tattatatat tccattagta ttaaagaatt gggtacagtc agattttagc
    21481 tggaaaacag atgtattatt gtatatcaga gattttctgt ttattggtag ttactatcac
    21541 ctgtggttct tgcctgcact ggcttttgct ctatggttag tacgcaaaag ccaggtttct
    21601 aaaataaagt ttctcagtgg tttgttgcta tgcgtagtag gtttagcatt tgaatcttat
    21661 tggtttatat tatctgggaa tgattccatc gagtatatta gatattgtta taaaagcgtg
    21721 tttgtaacaa ctcgtaatgg attatttata ggtgtaccat gcgtcattat tgggcaacac
    21781 ttagccttcc acaaaaacac tatacttaat aaggtcttta aataccgtaa ttttatctta
    21841 tgtgctgttt tagctatagg atgcttatta gttggtgaaa gtttcatcat aaatcaaata
    21901 gctttgcgag aagtcaataa tttgttaata ttttccattc cattagcact ggtagttgta
    21961 tcagtatcac tgagcctaaa gatagtacat ttataaaaat aacagtacat ctgcgaagaa
    22021 ttagtacgtt attattttgt ttacatccgt atataatagc tgctttaaat tttattttcc
    22081 caaaattggg gattacaaat tcattaatag aaatgacttg tggaatgata ataattttaa
    22141 tttctacagt ttttgtgtat gcgtgtagtg tgaaatttac cttgctgaaa aaattatatt
    22201 agattagtaa ctggtacatg taaatttgtt gaaattatag cagcatagag ataggaattc
    22261 tgttgagttt tattttcgct ggccgaggtt attcatgtta gagtgggtac ctccttttac
    22321 gaaaccttct tgatggtact catttagcgc atgatgaaag gagagtttat gaagaaaatc
    22381 atatcttcta ttttggttaa tgtgttatct gcattgtatc aaatatttgg gtttcgattt
    22441 taattgcagt tttggctatg tttttatact tgtttgcgat ggctgaggag aaatcaggat
    22501 taggtttgaa aaaatctttt cttgtctgga ttcggtattt cagaaaatca agctcattta
    22561 gaaaaatgtt tttgcttgtg ttttttacaa atatgattct ttttagaaca ctgatggata
    22621 gatatgtttg gctaaatcct ttgtctaatg tgtttggtag ttggtggatt tactctgtga
    22681 atgaaacgac aggggagatt agattaacta cagaatgttt tgaaaatgtt attctgttta
    22741 ttccatttat aattctagta atgtggtata aggcttcaga aaaagatttt ggaaaaattt
    22801 atcagagatt ataaaatttg tttttttatg ttcgtgtctt atagagtttt tgcagttatt
    22861 tttacacata gagacattcc aattgtcaga tttattttat aatactttgg gtggcggcat
    22921 tagtggggtt atatattatt tgagctgata tattcatcct aaccttaaaa ataaatgagc
    22981 agaattttaa aagattggag agattctatg gatcataatg caaattatta tcattggctt
    23041 acaaatgcca atgcagaaat tgtagaggaa ctgcggtcat attccgagaa ggacattgag
    23101 gactccttct ataaaaatct gtcctttggt accggtggtc tgcgtggcac cattggtgca
    23161 ggcactaatc gtatgaacgt acacaccgta ggtaaggcaa gccagggact gtcagactat
    23221 ctgaataaga cctatgaaac cccttctgtc gtcgtgggct atgattcccg aatcaagtcc
    23281 gatgtatttg caaaggttgc cgcggacatc tttgctgcaa acggtattca ggtgcatctg
    23341 tggccggagc tgctgcctgt gcctaccgta tcctttgcta tccgttatct gaaggcttct
    23401 gccggtgtta tgatcaccgc atcccataat ccttccaagt ataacggata taaggtctat
    23461 ggcgcaggcg gctgccagat caccaccgag gctgccgctg agattctggc tgaaattgaa
    23521 aagctggata tttttgcaga tgtgaaaatg ggcggcgacg cttccagaat cgcttctatc
    23581 cagccagagg ttctgactgc tttcattgaa gaagtaaagg gccagtcggt tctgtttggc
    23641 gaagaagtgg acaagaatgt tgctatcgtg tattctcctt tgaatggtac cggtctggtg
    23701 cctgttaccc gtactctggc agaaatgggc tacagcaatg tgaccgttgt cgaagagcag
    23761 cgtctgccag acggtaattt cccgacctgt ccttatccga atccggaaat taatgaagct
    23821 atgtctctgg gcatggagta tgccaagaag tataatgctg atctgctgct ggctaccgac
    23881 cctgactgcg accgtgtggg tatcgccgtg aagaatgcag aaggggatta tgtcctgctg
    23941 tccggtaacg aaactggtct gttgcttctg gactacatat gctctcagag aattaagcac
    24001 ggcaagatgc ccactgaccc tgtgatggtc aagaccatcg tgactatgga tctgggcgag
    24061 cggatcgctt ccaactatgg cgtgcgtact gtcgatgtgt tgaccggctt caagttcatc
    24121 ggcgaacaga ttggtatgct ggaaaaggca ggcaaggtgg acagctatat tttcggcttt
    24181 gaggaatcct acggctatct atccggcagc tatgtccgtg acaaggatgg tgtcaacgcc
    24241 gcttatctga tctgcgagat gttctcttac tacaagactc agggtatcag tctgtttgat
    24301 aagctgaacg agatttatgc taagtatggc tactgtttga acaccctgca ttcctttgag
    24361 tttgagggtg cggcaggttt taccaagatg cagaaaatta tggttgaatt ccataaaggt
    24421 cttgatatga ttggaggtaa gaaggtcatt gaaaccttgg attacagcac gggactgaat
    24481 ggcctgccga agtctgatgt tctcaaatat ctgctggagg gcaactgctc tgtagtggtt
    24541 cggccttccg gcactgagcc gaagctgaag acctatatca gcgttagcgc agaaagcaag
    24601 gaagctgccg aagtaatgga agctgttatg gctgctgaac tggctgaaat gtttaagtaa
    24661 tactatgaaa aagatctgag tgtcaatgtc cgaagtagca acatctatgt ttaataaaaa
    24721 tttcctgctt tacggttctg tttacataat tgaggtaaag gggccgactg tgtggatcga
    24781 aaaggcaaag aagtgttaac agagacacag cagcctgatg aagcaccgat caaagcagta
    24841 agggagaaat aagagatatt agagttttaa taatgtattc agcaatagag atgaaaggag
    24901 tagaatatga ataaaactat tgaaataata aaatgagggg gaaagaaata aacgtgatat
    24961 tattagtttt gagaaggcgt tttcaatatt cgctattgta ttgatgtact attttcagca
    25021 atcgtatttg ccagcaatac tgaacaaagc aatatctatt ggtgatacag gagtgcatat
    25081 attcttcttt tgctcaggtt ttgggctata tctgtcatat ttaaaaaagc ctattacatt
    25141 gggtgaattt ttacagaaac gatttatcaa gatttatatt ccgtatatta tcgtaattac
    25201 ggtatcagtg ttactacctt atatgacaca agatttaaat gctgcaaaag tatatttgtc
    25261 tcatgttttg ttatttaaaa tttttctacc agtatatgag gagtcactgg gtccctttgg
    25321 tttatttcga caattattca gttctatttc ttattcatac caattgtaaa actatagaga
    25381 aaaataggaa tgaaatgatt gcttgttgca agctgagtta ttagtatctg ggctattatg
    25441 atgtttcata caggatatgg aagcatacga gttcttggga gctgctttgc gcaatatatt
    25501 tgggaattta ctttcggcat gtttggttgc agagtattta gtgaaaagaa aaatagtaag
    25561 atttaataat atattcgtga ttatggcgtc cattgttgga attggttttg ctggtatatc
    25621 gaaaattatt ggtggactga tggagtctat taatgatatt tttgcattct tcggttacgg
    25681 tgcaattgca attttaatgt atcaagttat tccatttaga aagataatgc ctaggatttc
    25741 aagatactca tatgatatat atctactcca tattcttgta tttagtacga ttcatcaaat
    25801 tatccctaac ttggttttgg cggcatttgt ttccattcct ataacgttgg tgttgtcata
    25861 ttattatagt attatgttat atagacttaa attaaaatga tagcataatt gtcgcctcta
    25921 ccgggcggtt gccatcctga ttaacagcct ccacgcggcg gatggtgggc acttaggtag
    25981 ttcgtaacct agtggtagat ggttggtaaa aaggagataa aactaaatga tt
//
LOCUS       DACCQI010000035        25502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCQI010000035 DACCQI010000000
VERSION     DACCQI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.035"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IVO76_001101"
     CDS             133..3660
                     /locus_tag="IVO76_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7429074.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IVO76_001102"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IVO76_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7429075.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IVO76_001103"
     CDS             7448..7789
                     /locus_tag="IVO76_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7429076.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IVO76_001104"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IVO76_001104"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7429077.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IVO76_001105"
     CDS             9146..11566
                     /locus_tag="IVO76_001105"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7429078.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVO76_001106"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IVO76_001106"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7429079.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELDELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IVO76_001107"
     CDS             13000..13737
                     /locus_tag="IVO76_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7429080.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IVO76_001108"
     CDS             13751..14566
                     /locus_tag="IVO76_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7429081.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IVO76_001109"
     CDS             14579..15226
                     /locus_tag="IVO76_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7429082.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IVO76_001110"
     CDS             15242..15898
                     /locus_tag="IVO76_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7429083.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IVO76_001111"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IVO76_001111"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7429084.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IVO76_001112"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IVO76_001112"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7429085.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IVO76_001113"
     CDS             18240..18758
                     /locus_tag="IVO76_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7429086.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IVO76_001114"
     CDS             18865..19365
                     /locus_tag="IVO76_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7429087.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IVO76_001115"
     CDS             complement(19645..20565)
                     /locus_tag="IVO76_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7429088.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IVO76_001116"
     CDS             20730..21743
                     /locus_tag="IVO76_001116"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7429089.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IVO76_001117"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IVO76_001117"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7429090.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IVO76_001118"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IVO76_001118"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7429091.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IVO76_001119"
     CDS             complement(23383..24144)
                     /locus_tag="IVO76_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7429092.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="IVO76_001120"
     CDS             24333..25427
                     /locus_tag="IVO76_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7429093.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5383 g         7247 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcatctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DACCQI010000036        24818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCQI010000036 DACCQI010000000
VERSION     DACCQI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.036"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..213)
                     /locus_tag="IVO76_001121"
     CDS             complement(<1..213)
                     /locus_tag="IVO76_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429094.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIE"
     gene            complement(398..796)
                     /locus_tag="IVO76_001122"
     CDS             complement(398..796)
                     /locus_tag="IVO76_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP7429095.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(793..1566)
                     /locus_tag="IVO76_001123"
     CDS             complement(793..1566)
                     /locus_tag="IVO76_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7429096.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1563..2246)
                     /locus_tag="IVO76_001124"
     CDS             complement(1563..2246)
                     /locus_tag="IVO76_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7429097.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2413..3300)
                     /locus_tag="IVO76_001125"
     CDS             complement(2413..3300)
                     /locus_tag="IVO76_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7429098.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3297..4166)
                     /locus_tag="IVO76_001126"
     CDS             complement(3297..4166)
                     /locus_tag="IVO76_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7429099.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4303..5589)
                     /locus_tag="IVO76_001127"
     CDS             complement(4303..5589)
                     /locus_tag="IVO76_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7429100.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5862..6440
                     /locus_tag="IVO76_001128"
     CDS             5862..6440
                     /locus_tag="IVO76_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7429101.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6440..7369
                     /locus_tag="IVO76_001129"
     CDS             6440..7369
                     /locus_tag="IVO76_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7429102.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7366..9102
                     /locus_tag="IVO76_001130"
     CDS             7366..9102
                     /locus_tag="IVO76_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7429103.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9165..10001)
                     /gene="pheA"
                     /locus_tag="IVO76_001131"
     CDS             complement(9165..10001)
                     /gene="pheA"
                     /locus_tag="IVO76_001131"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7429104.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9998..10507)
                     /locus_tag="IVO76_001132"
     CDS             complement(9998..10507)
                     /locus_tag="IVO76_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7429105.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10500..11795)
                     /gene="aroA"
                     /locus_tag="IVO76_001133"
     CDS             complement(10500..11795)
                     /gene="aroA"
                     /locus_tag="IVO76_001133"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7429106.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11808..12899)
                     /locus_tag="IVO76_001134"
     CDS             complement(11808..12899)
                     /locus_tag="IVO76_001134"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7429107.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12916..14082)
                     /gene="aroC"
                     /locus_tag="IVO76_001135"
     CDS             complement(12916..14082)
                     /gene="aroC"
                     /locus_tag="IVO76_001135"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7429108.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14084..15148)
                     /gene="aroB"
                     /locus_tag="IVO76_001136"
     CDS             complement(14084..15148)
                     /gene="aroB"
                     /locus_tag="IVO76_001136"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7429109.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15181..16203)
                     /gene="aroF"
                     /locus_tag="IVO76_001137"
     CDS             complement(15181..16203)
                     /gene="aroF"
                     /locus_tag="IVO76_001137"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7429110.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16862..17836)
                     /locus_tag="IVO76_001138"
     CDS             complement(16862..17836)
                     /locus_tag="IVO76_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7429111.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17817..19700)
                     /locus_tag="IVO76_001139"
     CDS             complement(17817..19700)
                     /locus_tag="IVO76_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7429112.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19718..21943)
                     /locus_tag="IVO76_001140"
     CDS             complement(19718..21943)
                     /locus_tag="IVO76_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7429113.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            22058..23092
                     /locus_tag="IVO76_001141"
     CDS             22058..23092
                     /locus_tag="IVO76_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7429114.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(23159..24172)
                     /locus_tag="IVO76_001142"
     CDS             complement(23159..24172)
                     /locus_tag="IVO76_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7429115.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24250..24438)
                     /locus_tag="IVO76_001143"
     CDS             complement(24250..24438)
                     /locus_tag="IVO76_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7429116.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7139 a         5392 c         4427 g         7860 t
ORIGIN      
        1 ctcgatataa tcttttactg gtaattccaa ttctacctta aaaaagtgca aaacagccga
       61 cagcccttct gcttgataga tttctcctaa tgttagcccc tctagatgga actctggtga
      121 aaaatgcagc aatgttttat tttcctgcaa ttgaacatat agaaatgtag atcgctcaat
      181 cagagtatct tttaaaacaa ctaaaaaatt catttttctc cccctttttt catcattttt
      241 aaaatagcat attttgaaaa gaaagtctga tgcttagagt agaatttcat aaaaatttac
      301 tatttcataa gtattcacga gaaaaataca aacaaaaaag cggaaataac gctcatccgc
      361 cttcttgttt gtgtttattt cctcttcaaa cgcttgtcta tgattcagac ttttctctat
      421 cgttattttc atctaaaagt tcttgcattc cgataatttc tcgatattga tgaatcttag
      481 tgataccaat gactttgtct acatcaatac taaaacaaat accattacct ggaatatcga
      541 tgttgatgcc ttgaccaatc gcatccatta ctttattcgt caaactatcg ggtactaatg
      601 tcaacacaat atctttttct ggcgaaatgt ccaaacctaa aaatttccct gaatcatgta
      661 caccagaacc tcttccatgt aagatcgttc cgcctgctgc acctgcttct tttgaaaagt
      721 cgataacatc ttcgcccatt cctccgtcaa cgatcgtcac aatcatgtga agattcaatg
      781 ctacaagatc tttcatttgc tttcccccaa tttttgcaat ctttcttttt cgattctgtc
      841 tttttcatct tttcgtttat aaatatatcc tagtactaaa gtggataata ttggtgtcaa
      901 tgcaatcatt gcaaccacac caaacccgtc aatgatcgga tctcttcctt caatttgtgc
      961 agcgattgcc acgctcattg ataaaataaa tgtcgaacac atcgggcccg ttgctactcc
     1021 accgttatca aaagccattg ctaaaaagat cttgtccact tttcgtccta aaataaatgc
     1081 tagcccgtat cctgggatca agaaataata aagagagaat ccgacgagca aacgccacat
     1141 agacaatcct actgctgcgc cgatcccaat tgaaattacc atcagcatga ttttcgatcg
     1201 gattctcccc tcgctcaatt cttcaacctg tttgatcatt acatgaatcg ccggctcagc
     1261 aaaccctact aagaacccca ttaaaaagcc aaggggaacc agtatataat tgtaaggcaa
     1321 tcccgcaata gcactgccta aatgctgtcc taccggtaca taagccatat tgactccgtg
     1381 caggaaaagg atcaacccaa tagtcgttat caagaaacct ttcataatgt caaagaaacg
     1441 ctttttgcgc atacgaaaac taatgatatt cattaaaaca aataggatta cgataggtgc
     1501 cactgccaaa atgacttcca tggtaacgat ttcgattcca tctatcactt gctgccagaa
     1561 actcattgga agatcacccc caaaatcatc acggcaagaa tcggccctaa cgaagcaata
     1621 ccgaccattc caaaactgtc attttcattt ttcttttgtt tgatcatact agtcatccct
     1681 ccggcaaggg ccaagataaa tggaacagtt actggaccag tagtcactcc ccccgaatca
     1741 aaagcaatcg gcataaattc ggaactggtg aaaaatgagg cgatcaaaac gcctatgtag
     1801 cctatagcca tcaattgata ataggatagc ttgaatacga cccgaagcag cgcaaaggct
     1861 agaaatactc cagtgccgac gctgacgatc ccaatcagca acactctgcc aatctctccg
     1921 tcagatatat catatacttg ttgtcccaaa acttgcacgg aaggttcagc aatcgtgatc
     1981 acgatcccga tagcaaatcc caaactgacc aaaacggtca aacttttctt tttgatcata
     2041 tatttcccta ctagatctcc gacttccatc atagaataat ctgctccaaa caaaaagagc
     2101 gtcattccga tcatcatgat tgccgcacca accagaaatg aagcgaggtc ttctccttct
     2161 aacggtgcaa aaacaaacgt taaaatgaca atcaggacag tcatcggcaa tatagcagca
     2221 atcacttctt taaaattttt tagcatgtat cctcctcctt tactctttaa gtttacctta
     2281 aaatgaaaga gttttcgagc acaaacttaa aacgttatca tttaattttt ctcaacataa
     2341 aataacacac gtcccaaaag tatcttttta cttggaaaaa tgactttgaa ggaacgcgct
     2401 tattgtgttt ttttatttta cagagccgat cattccttga acgaattgct tttgcaaaac
     2461 aaaaaatacg atggcagttg gtaaagtagc gatgactaat ccagccatga tcattccata
     2521 gtctggtgta taggaagcac ccatagcaga taaaacaagc ggaaccgtcc gtttatcagg
     2581 agattgcaaa gctactaagg gccataaata attgttccag ctactcatga atgtgatgat
     2641 tgcagccgcg gcataggtat ttttagccgt cggcatatac actctaaaaa aaatacttag
     2701 ttcttttaga ccgtctaatc gagcggcttc tactagatct tttggaaaag atttaacatt
     2761 ttgtcgaaag aagaagatca aaaaagcagt gctaacagat ggcaggatga caaccaagaa
     2821 actgttgatt cccaaaaccg ttccgttcaa ttgtgaaaac attcgatata aagggatcat
     2881 caatgccgca aaaggaacca tcatcgatag cagtagaata ttgaaaatta ggtcttttcg
     2941 tttactttga taaatctcaa atccatatcc tgccattgac gaaatcagca atgcaaacac
     3001 agtggtcacc aaagcgatca tcgctgaatt ccaaagggaa cgagtaaaat tcagctcatt
     3061 ggaaaacaaa ttttgaaaat tgatgatcaa gttactgcca atctttattt ttcccgatgt
     3121 gatatccacg ggtgtattcg tcattcccaa aaccatccag acaaaaggga aaatggaaat
     3181 aaatgatagg atgcttaatg ctgtatactt taatactgac ccaattgtcg aacgaagttc
     3241 taatgcttgt tttttgacgt tggggcgttc gattgtttct ttttctacca tatttttcat
     3301 cgactttctc ctcctacttt cgtttggatt atcgacaata tcacaataaa ggaaacaata
     3361 acaaaggaaa ctgctgccgc atatccaaaa ttaggcgtga atttataact caaattatag
     3421 atgtattgag atagggtggt cgttgcattt gctggtcccc cgccagtaat attttgtact
     3481 tcatcaaaaa gctgcaatgt cccgatcgtc gaagtaatgg aagtaaacaa aatgattggt
     3541 tttaaatttg ggatcgtcac tttgataaac tgctgccatt tagaagcacc gtcaatagat
     3601 gctgcttcat aaatctccga atcaatattt tgcatgcctg aaaggaaaaa aatcatattg
     3661 tatccagtcc aacgccaagt gatcgaaaga atgatcagaa ctctcgccca aattggatga
     3721 gtcaaccagc caatcggtgt gtcaatcata ccaatgccca acaacaagtt gtttacaagg
     3781 ccagattgag aaaatagact tttcatgatc agagaataac tgaccatcga agtgatacat
     3841 ggaagaaaga tagcggttcg aaacaaccct ttatatttca actttttatc atttaaaagg
     3901 ttggaaataa aaagtgctag aaacaacatg atcggtactt gtacaagtaa atacaggaat
     3961 gtattaaaaa aagctttttt aaaagttgca tctgtcaaca tccgctgata attacctaac
     4021 ccgttaaatg tcatattgac agggggacca ctttgaaaag acgtgatcaa agccaaaacc
     4081 atcggaataa aaaccatgat tgatatcaaa atgactggca agattaaaaa agcatgcccg
     4141 tttttgaata aatctttctg cttcattctt ttccctccaa ttctatcatt gattatctac
     4201 atgaaaagac aagaaagaca atatctgtaa ggcagacgtg atcttttttg tcttttcgtt
     4261 tgtctgatga aaaagcgaag aaattcactt ttttaccacc gatcaattag ccaattgtgc
     4321 ttctacttgt ttttgtgtat ctgctagtac cttgtctgtc tcttctccat tgatgattcg
     4381 atgaagagct tcagctacga ctgcttcaat tgcataggtc tcatgtccgt aattcacttc
     4441 tggtatttca gatgtccatg ttgaaaaatc gtcaaagatc ttttgaccac tgtaaaattc
     4501 agaagcttct tgataagctt cttgttcatt cgcgctaagc atcgtagata ctagtccgat
     4561 ttctttcgca agagtgccca tcagttcttc attcgaagca aacgttgctt ctaggaaatc
     4621 tttcgctgaa tcttctccag ctactccttt gatcacatac cagccagcgc cgccaaggtt
     4681 agaagcttgc gctttttgca gattttctgg aagtgcaggt attggtgcaa tcttccattt
     4741 tccagattgg ttttctgctc cttggatcgt tgaagaatac caagcgccag ttgggctaga
     4801 agctactgct cctgattgta tcgcagttac tccagcattc cagtcagagg tttgttcgac
     4861 tagatcttct ttcaaaagag tggcaaacaa ttcaagacca tactttaaag atgcattgtc
     4921 ttggatcgtg actgtctccc catcttcaga tgtataccat tctcctgcct gttgcataat
     4981 catccttact cgaccaaggt cagaaggatt gacttcagcg attttctttc ccgtcttttc
     5041 tttgacatct cgcgctactt gaatatactc gtcccacttc agattctcca tatcctcttc
     5101 tgtataacct gcttctgcca tcagatccgt acgatagaag tttgctgtta cgcccgaatc
     5161 gaaaggaaca ccgtatattt tatctcctac tttgtttacc gcaaatttat acggtgcaaa
     5221 atgatcttcc tttacaatag cagacaaatt agcgaatgca tctggataag aagtcaaata
     5281 gccttgtatc ctataatctt caatcaagac gatattgggc aatccttctg tattcccgct
     5341 cgctaaagcg gtgttcagtt tctgaacgat atcatcctga gacatcgtaa caacttctat
     5401 ctctgtttct tcgttctcat aaactttttt tgcttcattc actgctttga tattgaatgt
     5461 ctcatcccac gcccagacag tgactttttc ttctttgtct gctgtactac tagcacttcc
     5521 gcatcctgtt aagaaacctg atgctgccgc taaacaaacg actccagcaa ttaactttct
     5581 gatgttcatt tttatccgct ccttttccat ccattagttg tttacgtttg ctacattcat
     5641 cataccaaaa tttgaaaacg gtttctttat tattcagata cttcaaaaat ttctcctaaa
     5701 ataataaaaa aatacaaatc aactatgaca aaaggaataa tcttagcatc tagaccaaag
     5761 atcgaaatgg tgaggtttct ttaaaataaa aatatccttg cattttctcc tagaaataag
     5821 tatttttaaa ttacaagact attcttaagg cgggtaaaaa aatgccaata cgcgtaattc
     5881 ttatcgatga tgaacagacg attttagatg gaatgaaaca gttgatcaat tggtctgagc
     5941 acgggtttca attaataggg actttttcca gtccgctgaa agcgcttgat ttttctttgg
     6001 tacatgcaat cgatatcgtg gtcacagatg taatgatgcc tgaattaaat ggaatcgaat
     6061 tatcgcggct attaaaacag caacaacctg aaacacagat ccttatcctc tctagctatg
     6121 atgaatttga tatggttaaa gattctttta aagaaggcgt cgctgattat cttttaaagc
     6181 ctcgattgag tcctgatttt tttctaaatt ccttaactgc cgtttcacaa aaaatcaaaa
     6241 aaaagcccaa caaaagccca atcaccaatc gttatgagca gatctgcgag aatctttcac
     6301 agcagattgc tggacaaact cgtccattgc ttgaccctct tctttttgaa cacaccttgt
     6361 ttttctgtta tataccgaaa aacggccatt cagttcaaaa caaggctcta aacaaaaaca
     6421 gtcgatcacc cattcattga tggatagcga cgattcgttt actatttatc ctgtttctac
     6481 cttttcggaa gaatccggct atgtaatcaa tactgattcc aaagatacac ttaggaagtt
     6541 tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt tttgttgttt ctgattcttt
     6601 tgcaatccaa gatatcaatg aaatattcca agagatcaga aattgcacca aaggacaacg
     6661 tttctatcat aagggaaaga accttgctta tcagcatgaa cttcttccgt tattaaccaa
     6721 ccacgagcag caagcaagta gttatttaac aaaaatcgtt cataaagact acgctttagc
     6781 tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg atccagctta
     6841 tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg ctgacgagca
     6901 tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat tagccgatgc
     6961 tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac tcacccactt
     7021 ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg agttcgtcca
     7081 ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc atttttcata
     7141 tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa aatatctaaa
     7201 aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta ctttgacgga
     7261 aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt ttaaagaaga
     7321 gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag aaaaaaaatc
     7381 aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct ttttctgcaa
     7441 gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat ccagctgttc
     7501 aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag tttgaatgat
     7561 acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag ctatttttcc
     7621 aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca aaaaatgaat
     7681 gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt tggagaaaat
     7741 ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc tttttttcaa
     7801 tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct ttttgcacac
     7861 cacggattga ctcttcgcga taagaaagca cccagcgtct tctttgtcaa aaaactgaaa
     7921 aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa gcaactagcg
     7981 aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat gaacgaagaa
     8041 cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat gattttcaaa
     8101 aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg tcttcctctg
     8161 taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc taaccaaatg
     8221 ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc tggctcattg
     8281 gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga ttcgctaaaa
     8341 cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca tgaagtccaa
     8401 ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta tgaacagacg
     8461 atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc acagatacag
     8521 ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca gaaaaaaaca
     8581 gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac gattgggaaa
     8641 aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta catcgatctt
     8701 ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga tgaactgcag
     8761 tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta tcttcatgct
     8821 tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa tcagcttctt
     8881 ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa acaaaaaaat
     8941 gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct aatgtatggc
     9001 gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt aacgatttgt
     9061 ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc caagcgctga
     9121 tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct acgcgtctct
     9181 tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag catcttgatt
     9241 tcttggagcg cattctctac taatgtcatt ggacggtcca acaacaaatc gatcacgaaa
     9301 aaatattctc ccaagctagt tttcaatggt cgggattcaa tcttacttaa gttgatttcc
     9361 cgccagccaa aagctgccag cattttatga agcatccctg gccgattagc aggtagtgtc
     9421 aagatgacac tcatttttac tggagctgac tgctggctgg tcatctttcg gtcaccgacg
     9481 atccaaaaac gtgtctggtt caactcatta tcttggatat tctcagctaa gatctccaat
     9541 ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt cggatgttct
     9601 gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac attaggataa
     9661 tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgcgaaag gatttttgta
     9721 atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat ttcactttta
     9781 acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacagc tccttccaat
     9841 gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc gcgcaaacaa
     9901 acagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca agccgcctga
     9961 taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg atcctttcta
    10021 taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga tgtgtcgcac
    10081 tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct cttgtttttc
    10141 caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact agacagtccg
    10201 cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga ttttctgcac
    10261 tttggacaat ccctccacca gtcgcgataa ttttgtcttc atttgacagc tttcttaaaa
    10321 tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat tcggcaatcg
    10381 acaattggat atgttcctca atctttgtat ctaaatctac atactctttt tgtaaacatt
    10441 gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc agcaaaatgc
    10501 tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa tgccggataa
    10561 gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat caaagcagct
    10621 acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc gccatgcaga
    10681 ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc tcccattttc
    10741 tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa ttcttctgcg
    10801 tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat gggcaattca
    10861 tcaatcagac gcggaatgat ttctccacca atcgttgctt tcttcaaatg actcgtcttg
    10921 acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat cgctgcgccc
    10981 atctcctcca acacatctaa gataccagtt cttgttggat tgaccccgac attttttaac
    11041 aacagttggc tttccggaac aagaagtcct gctgctagaa agaaagcagc tgatgaaata
    11101 tcacctggaa cagttatctg ttgaccagtt aatttttgcg gtccggtcac tatgatcgtt
    11161 ttatcttcca ctgtgatcct tccgccaaat tgacgaatca tttcttccgt atggttacgg
    11221 gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc aaacagcaaa
    11281 gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga taaagagcgg
    11341 gttcctgtca cagtgattgg tagatactgg tcattttctt ctccttgcaa gtcagcgccc
    11401 atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga agcatccccg
    11461 aacagagtag tcgtaaaagg ctgtccagcc aaaataccca tcaataaacg tatggtcgta
    11521 ccggagtttc ccatgtcaat tgattcggtt ggttggacaa atgagtggct accgcgacca
    11581 tgaatcttga tcacctcttc ctcttcttcg atctcgacac ctaattgttg gaaggcatgt
    11641 aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt tccttcagca
    11701 attgctccaa acatgatgct gcgatgggaa attgatttat ctgctggtat cctaacagtc
    11761 cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta tttgatccag
    11821 caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc ctgttggttt
    11881 ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat cttcaaatta
    11941 atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac gccaggttca
    12001 tctggcacat cgataaataa atcaaaaaag gcaggaatcg cgccttgctt atggacaggc
    12061 aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga ttggttctca
    12121 gtcatgatcc attctgctat ttgtgtcatt tcttgttgcc aatctgccaa tcgttccaat
    12181 aatatctgct tattgctcaa caaaatatct gtccacatga caggatctga ggaagcgatc
    12241 ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata ctcttgattg
    12301 aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg acttagcatt
    12361 cctgttatac ggtcatgttc tcttgctgta agagttacat attttgcttt ggttccgcaa
    12421 aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt aaagatgtaa
    12481 taggcatttt caaatagttt agcatctgct gcctgtatac ctgatttatg agaaccagcc
    12541 attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac gacttgctgc
    12601 ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct ttcaaggaca
    12661 tcaagataag ctaaggttgt acggacggga acggctaata agataacgtc tgcttgttct
    12721 gcacctgttt caaaatcact tgcaacgtaa tcaacgatct gctgttctac tgcttgctca
    12781 cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt ctgacgaata
    12841 caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg ttgtgccatc
    12901 ttatttgccc cctttttagt aatgtttcaa gtagtcacgg tactccttca ctgcagcttt
    12961 caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta ccacagtggc
    13021 tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg aacgttctac
    13081 acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct tgtacaatgt
    13141 aggaatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca ttccgccttc
    13201 gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc ataggatctc
    13261 atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa attctacgcc
    13321 tttaaaggca ttgatactaa ccacagcttg tgcgattttg gcatctagct tcgtgtccca
    13381 ttgtacgtag cttcctaatc cagctggaac gcctccaacg agcacttcta caattcctcc
    13441 gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt gctcaactgc
    13501 tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt gaatcgagat
    13561 atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg ctacgatgat
    13621 gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca tggtcgtctc
    13681 tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt tcataccgcc
    13741 taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt ggtctttttc
    13801 aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat tttctaccac
    13861 taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga tccgaacacg
    13921 atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc gagcgagttc
    13981 gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa taatggctgt
    14041 taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt cctcctaagt
    14101 aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc ttttctagca
    14161 agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg tggaataatt
    14221 catctggatg acttagatcg tacgtaatcg gcagatggaa tttttgaagt atcctttcta
    14281 aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa gtgatggcta
    14341 ccattccaat agctactcct tctccatgac tgactacccc atatcctgct gtattttcaa
    14401 ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg tcaaagacat
    14461 cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga acagcatgtt
    14521 ccagaagatc tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt gcatctgcta
    14581 tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc ggcaaagttt
    14641 ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg acaaggtttt
    14701 tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact tgtgcaagta
    14761 gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc acgaatgctg
    14821 ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg gtaaaatcat
    14881 tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg gtctcacccg
    14941 ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac atgatttttt
    15001 ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga ggtctccaaa
    15061 tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga atatcgtatt
    15121 tttggttatc aacatgtaca gtaagcattc gtttcacttc tttcttttaa aaagctggaa
    15181 ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt ctttcatcat
    15241 gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat ttgcaggatc
    15301 aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac gagccattgg
    15361 tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag ggaaatgagt
    15421 taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg ctgtttcgta
    15481 agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg caatgtattc
    15541 tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc caacaggttt
    15601 acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg cgccaatctg
    15661 taggatatcg ctgtactcag ccaccatatc gatatgccct tcatccatca cttcagtaat
    15721 cactttcatt ccaaattcat cagccgcttg acggatgtat ttcaatcctt cttcttctaa
    15781 accttggaaa gcatatggag aagtacgtgg tttgaaggca ccacctcgca gaatttttgc
    15841 gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt cgatagaaca
    15901 aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta catccacgac
    15961 tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa tacgcacagc
    16021 attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg tatcaccttt
    16081 tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc cttctttttc
    16141 gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca taattactat
    16201 cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt ctctactttt
    16261 tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc tcattttcgc
    16321 tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct gtaggaaatt
    16381 gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata taaaatagcc
    16441 actatagatg aatttctcta cgtggctgta agtcgtgttt aaaaatcttt agccatcata
    16501 gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat ctaaaatagc
    16561 taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat cgctccaaac
    16621 tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc atttttatga
    16681 aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca attggtttta
    16741 ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct tgttcatgac
    16801 atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa tctaaagatt
    16861 gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg taaggtgact
    16921 caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca aaaatcgtaa
    16981 gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc agttcttctg
    17041 ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat cgcaacgaaa
    17101 aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt tctacccatt
    17161 tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg gggagtcctg
    17221 ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt tcccctgata
    17281 ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa cccaatgcgg
    17341 gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt ttaccctctc
    17401 cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt tccagtgtaa
    17461 aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt actggagcaa
    17521 tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact tttggataca
    17581 atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg tcattatcgg
    17641 tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt ccgtttttat
    17701 tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc cccgatttga
    17761 cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata tgacttttat
    17821 ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc ttgtccatct
    17881 gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc aaagtcaccg
    17941 ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc ctgatcttta
    18001 aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt catgtaagaa
    18061 tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat gaacggtttt
    18121 tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat gcagcttttt
    18181 acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata atggacaagt
    18241 cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc ttccgcataa
    18301 gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt ttcgaaattc
    18361 gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg tacgttgtaa
    18421 gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt cgtttccgcc
    18481 atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt ggggtaatga
    18541 gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt ttccatatct
    18601 tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac cccgttgata
    18661 atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt acgaaaaccg
    18721 aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc caattcaagc
    18781 ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc atcgatgcga
    18841 atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc atcaggatct
    18901 agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa atctacttca
    18961 agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc ttctaaatgg
    19021 ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg gaaaaagtcc
    19081 tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag aatattttct
    19141 tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc ggcatacccg
    19201 ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa atgaatcgag
    19261 atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga accaaccgga
    19321 ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt cgtgaaaggg
    19381 ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg gatcttgtca
    19441 taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca ggcatcaaaa
    19501 tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca aaaacgccat
    19561 gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct tgttgcataa
    19621 caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg atcatctaac
    19681 cattttatat ttgctttcat acttttttcc ttctctctca taactttttg aaatgaagca
    19741 gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc atcaattcgg
    19801 cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca tctaacccca
    19861 tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct aaaaacacaa
    19921 tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct tcaaaaggac
    19981 tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga taggatataa
    20041 tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc agttcataac
    20101 caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat ctgtcggtct
    20161 gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa gcaaggcttg
    20221 ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc catgactgcg
    20281 gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag ccttcccata
    20341 agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg agcatataac
    20401 gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca tctgaaagcg
    20461 aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc tttctcattt
    20521 gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt tgtgatctgg
    20581 aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt cgatacagtt
    20641 cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat cctttgctat
    20701 gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac tcgaaccagt
    20761 ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct aatacgaaca
    20821 tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca ctattgaagt
    20881 caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat tgatgacgac
    20941 ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg ttcaatccct
    21001 gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca ttcaattccc
    21061 acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc tgatcttttt
    21121 caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct tctccagtat
    21181 tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga ctgctcgttc
    21241 ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa atctgtcttt
    21301 cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta tgggcaaagt
    21361 ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca tttcttatct
    21421 ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa tcgaaataaa
    21481 tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac ttgtccatca
    21541 cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa tggctgtcat
    21601 atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg ggcgttcgaa
    21661 agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac agagaccggt
    21721 caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa tttttattgt
    21781 ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg ctttcttcta
    21841 tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc ttttcatcaa
    21901 ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca ctcccaacaa
    21961 aacggaaacc gtttactttt ttttataatt cctgtatact gtttactaaa caattttgtc
    22021 aataaaaatt ttttattttt ttgaggagtg tgtaaaaatg gttggaatta gagatgtcgc
    22081 acgaaaggca aatgtctctc ctgctacagt ttcccgtgtg ttgaataacg atcagacgat
    22141 ccatgtggct cagactactc ggcaacggat agtagaggca gctgctgcgt tggattatga
    22201 catcagtact cgtaaatata tcaaaaagag aatgccttcg attggtgtga tctctacgat
    22261 cggcaaagcg agcgaagaac aggatatcta ttacaaacat ttgcgagtag ggatggaaga
    22321 agaagctcga cgtctgcatt tagggatgaa tcgaatctat aatctatctg ataatcctaa
    22381 acaatgggaa gatctagatc agttaggtgc ggtgatcgtt gttgggactg ttacgaaaga
    22441 atcgattacc gccttgttaa aacaaaacat gcaaatagtc gtcgtggata accctgacat
    22501 ccagcaagag gtcgatatgg tgtatgcaga tcttgaacgg atgactaaaa ccgtactgga
    22561 attgttctta gaaaatggtc ataaaagaat agcctatatc gggggatata aagtcgatat
    22621 ggatgaattc ggtgggaagc agatttctat aaatgaaaag aggatgcgtg cctatcagca
    22681 ttttatgatc gatcatcact tagaaaaata tatgtcttat catctaggtg gctggacaga
    22741 aaaacaaggt gaggtacttg cgactaatct attagaaaat gaatcccctc ttcctactgc
    22801 cattcttgtc gggagcgatc caatggcaat tggtgtctat cgggcattga aacaaaaaaa
    22861 tatccaaatt ggagaagaga ttgttgtagc aagctttgat aatatcgaga caatcgcaaa
    22921 cctctcccct ggcttgacca gtgttcaaat caatgcgaaa tcaattgggc aagcagctgt
    22981 tcgaatggca ttggagcgaa ttgacggtat acgggacgaa ccaatcattt tgacttatcc
    23041 tgcacgattg attgttaggg aaagttttat cccaagaaca aaaaggtatt gaaagagagg
    23101 ttgtgaaaag ccgcttcgtt tcttcatact caaaacgaaa cggctttttc acaacctctt
    23161 acttatactt attcacaaaa tccaatgtct gctggtaaca attttgcgat tcttttagcg
    23221 tgtaatcaaa ttggtattca tgggtaatct ctttttttgt atctgtataa aacaaggatt
    23281 gcacgggtac ctttaattct gtcaaccgat tttctaatgc ttgaccttgc tcctggaatg
    23341 agtatgcatt tccgtcagtt atatacgttg gcggaaattc cttagaaaca tggtcagcca
    23401 cactagcttc tttcaattgc tcactcgttt tccaatcttt tgttccaatc aacgaccacg
    23461 caacggtttt gacgaaaaac ttcataaatc gatcatccga atgctgctgt gctgttagct
    23521 tcaaatcaac aggtccgcaa taagaaatgg ttcctttcag actatctttt ggaagtagct
    23581 gtttgatttt catttgttta gcatacgcat cattcgtttg tatagctgca tattgcaatg
    23641 cgatttgtga acctgcacta tctccaccaa aaaataattg ttccatgtct agcaatttgt
    23701 cgtctttttc ttcagctaat aaatctttga tcagttcatt cacttgaaga acttgattag
    23761 ggtattctga atcaggcgct aattcataat tcatcgcaac cactgcgatg ttggcgtctg
    23821 aaactaattt tgtcgcaaat tctttgacac ccgatttatc tccgccgacg aatcctccac
    23881 catgtaccca aaatagaaca ggaacaggcc ccttgctgtt ttttggatag tagatgtcat
    23941 aagtattctc tttatacttg gactcatacg tcaaatctgt ttttttcgcc acgttttttt
    24001 ctgcttgatc gaacgttttt ttatcagtga tttgaacagt atttgaaaac atatggttga
    24061 tcaagaatgc tccaggtctg ggggaaagct ggaaggcaag aaagattgca aacgcgatcc
    24121 caagaatgat tccgaagaac catccgataa atttaagaat tttctttttc ataaggctcc
    24181 ctcagtagtt agtattaagc attctgatta taccatagtg ttatttccta tcaagctctc
    24241 ctagatgatt taatgatttt ccccaaactt cttctgcaat gcttttgaca aaagaaattt
    24301 tttccattgt tcttcaaaca aaacattttc ttcttctgtt gaagcaaatc cacattgagg
    24361 acttaagcac aaacgatcca aaggcagata tcgtgtcgct tcgtagatcc ggtcaatcac
    24421 ttgctggtgc tcttccattt cgccattttt cgttacagtc aaaatctaag acaactaatt
    24481 tatcttcaga gactttctct aaaagaacaa aactcccagc acgttcacta tcgtattcta
    24541 aataataggc acggacattt tcttgagtaa aataatgggt ctgctacgtc gtcatccccc
    24601 ctgatgccgc caaaatcgaa taataatttc cgcgtcaaac atgtatattt agtgttgaat
    24661 catttgagaa tatgaaaata aaaatctttt tgatcaatca gttaatcaaa ttgaaataac
    24721 gaaaaatctg agacagaatg acgttcattc tgacccagat ttgattttgc acaactcaac
    24781 agtccttatt ggcaaatcgc caaaataatg tagactaa
//
LOCUS       DACCQI010000037        24396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCQI010000037 DACCQI010000000
VERSION     DACCQI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.037"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..642)
                     /locus_tag="IVO76_001144"
     CDS             complement(<1..642)
                     /locus_tag="IVO76_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7429117.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGY"
     gene            complement(948..1895)
                     /locus_tag="IVO76_001145"
     CDS             complement(948..1895)
                     /locus_tag="IVO76_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7429118.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            2085..2354
                     /gene="rpsN"
                     /locus_tag="IVO76_001146"
     CDS             2085..2354
                     /gene="rpsN"
                     /locus_tag="IVO76_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7429119.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(2530..2892)
                     /locus_tag="IVO76_001147"
     CDS             complement(2530..2892)
                     /locus_tag="IVO76_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7429120.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(2903..4024)
                     /locus_tag="IVO76_001148"
     CDS             complement(2903..4024)
                     /locus_tag="IVO76_001148"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7429121.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(4040..4390)
                     /locus_tag="IVO76_001149"
     CDS             complement(4040..4390)
                     /locus_tag="IVO76_001149"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7429122.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(4520..6031)
                     /locus_tag="IVO76_001150"
     CDS             complement(4520..6031)
                     /locus_tag="IVO76_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7429123.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(6353..7705)
                     /locus_tag="IVO76_001151"
     CDS             complement(6353..7705)
                     /locus_tag="IVO76_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7429124.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(7858..8934)
                     /locus_tag="IVO76_001152"
     CDS             complement(7858..8934)
                     /locus_tag="IVO76_001152"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7429125.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            9130..10875
                     /locus_tag="IVO76_001153"
     CDS             9130..10875
                     /locus_tag="IVO76_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7429126.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(11155..11592)
                     /locus_tag="IVO76_001154"
     CDS             complement(11155..11592)
                     /locus_tag="IVO76_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7429127.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(11576..12949)
                     /locus_tag="IVO76_001155"
     CDS             complement(11576..12949)
                     /locus_tag="IVO76_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429128.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(13271..13759)
                     /locus_tag="IVO76_001156"
     CDS             complement(13271..13759)
                     /locus_tag="IVO76_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7429129.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(13781..14383)
                     /locus_tag="IVO76_001157"
     CDS             complement(13781..14383)
                     /locus_tag="IVO76_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7429130.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(14887..15516)
                     /gene="udk"
                     /locus_tag="IVO76_001158"
     CDS             complement(14887..15516)
                     /gene="udk"
                     /locus_tag="IVO76_001158"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7429131.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(15600..16295)
                     /locus_tag="IVO76_001159"
     CDS             complement(15600..16295)
                     /locus_tag="IVO76_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7429132.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(16308..17024)
                     /locus_tag="IVO76_001160"
     CDS             complement(16308..17024)
                     /locus_tag="IVO76_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7429133.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            17354..17959
                     /locus_tag="IVO76_001161"
     CDS             17354..17959
                     /locus_tag="IVO76_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7429134.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            18284..18607
                     /locus_tag="IVO76_001162"
     CDS             18284..18607
                     /locus_tag="IVO76_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7429135.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            18789..20159
                     /locus_tag="IVO76_001163"
     CDS             18789..20159
                     /locus_tag="IVO76_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7429136.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(20289..21590)
                     /locus_tag="IVO76_001164"
     CDS             complement(20289..21590)
                     /locus_tag="IVO76_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7429137.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(21820..23811)
                     /locus_tag="IVO76_001165"
     CDS             complement(21820..23811)
                     /locus_tag="IVO76_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7429138.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(23955..24221)
                     /locus_tag="IVO76_001166"
     CDS             complement(23955..24221)
                     /locus_tag="IVO76_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7429139.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         7033 a         5178 c         4413 g         7772 t
ORIGIN      
        1 gtagccgtcg atcgtgtcca aattttcatc tgttgataaa atcatatctt taactttatt
       61 gatacttact aggttcgtat cgatgtcgta acctatctca ttgaatactt tcaataaagt
      121 cgaactggct gttttgataa attcatttgt gatttgcgat tggatcgaac ttgctccttt
      181 atcggtgatt ttcggtgcaa tcgcattgat tttttcattt accgtatatt cgatttttgg
      241 tttcttgata tcaccactag taaaactcag cagatcttct gagaaatcct ttggcaagta
      301 aatacctgcg taatactttc cggaacggac tccgtcttct aattgttctt ttgaatcaac
      361 gaattgccat cctaattgtt tatttttatg aagcgattcg atgacttgct ccccaatggc
      421 cacttcttta ccttgaaact ctgctggctt gtctgcactg taaacagcaa ttggcagctc
      481 tccagtattt ccatatggat cccataatgc tttgatgtta aaccacgcat atagcgaagg
      541 gatgatcatg atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca
      601 gtcaagcaaa tataatcgaa gcgtattttt gattgactgc atagttctcc cccttcttct
      661 tttttcagct gaattacttt accatgtatt tcatactttt ttcaatcggc ttttttcaaa
      721 aaagtcgaat ttttggacat atcacggcaa ttgtctcatt gtaatctctt acatattgag
      781 aaataattaa ggattacagc ctttacttta tcatgaaata aaagggaaag ataattttat
      841 gccaaagttt ttatctatta tacgtatacg caaaaaagct aaaacatttt ttgtctttgc
      901 ccctacaaaa atgtttcagc ttttttcgtt ttttattcct gtatttctta tttttcgaat
      961 gacaggatga ttttcccagc aggaggatgt ccttcaacta gctgatgtgc ttggatgaca
     1021 tgcttaagtg aagcagacag attcattgaa ataaaaacat gaaaggctcc tttttggtag
     1081 gctttgatta tgccagcaaa ggcttctgca tctagatatt cctttcttgg aacaaacgat
     1141 tcataaaagc cttccttttt ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca
     1201 tacctgccgc ctggtttcat gatctggatg ccggtttctc ctttgatact tcctttcgtc
     1261 gcatcgatta caaggtctgc ctgatcagca aattgcagac ctggatttgt tttatcataa
     1321 gcagcaaatg ctgaagctcc taattttttt accttttcct catttttact ggatgcactg
     1381 gttaagattc ggattccctt ttcatggaga agctggatca gacttgaccc gaccgcacct
     1441 gatgctccta gtatcattac agtatctgtc ggctggattt ccgtcaaatg gttgattaga
     1501 ttataagccg taattcctgg cgtgaccatt ccggctgctt cttcccaact cattttatct
     1561 ggaatttttg ctacttttgc tgatggcaac acgacttctt ctccgtaagt cccgccgact
     1621 gcatggacag ctactcgatc acctacatga acagttgtga catctgaacc aatctctgtc
     1681 acaataccag cgccatcatt acctggtaca taagggaatt tcaatgttcg tatctctttc
     1741 atttctccta aacgcaaggc aacatcataa ggattcacac tgaatgcttt tatttctaca
     1801 cgaacatgtc cagaaccgac ttctctggga tgagcatcga tttcttcaaa aacatcttgt
     1861 gcttctccgt aaccgttaat tgcaaatctt cgcatggtca ccatctccct ttttctttaa
     1921 gtttacgttt atgtctttat tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc
     1981 aaaacaaaat agttgtcttt tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa
     2041 gattacgatt tacgctaata aaaaataatt ggaggaattt gttgatggct aaaaaatcaa
     2101 agattgccaa agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct ttacgccagg
     2161 aattaaaagc gcaaaaagat tacgaaggct tgcgtaaact tcctttagat gctcgtccag
     2221 aacgattgaa aaatcgtgat ttaatcgatg gacgccctcg cggatacatg cgtaaattcg
     2281 ggatgtcccg tataaaattc cgtgaattag cacataaagg tttgattcca ggagtaaaaa
     2341 aagccagctg gtaatccaag tctggctgta atgtaaagga ggagggcgca tggaccactc
     2401 cttggatcat caaaaaagat acgttcgctt cttctagcag aaatgctggt actagaagca
     2461 gacgtatctt tttttacgca cagctggtcc ttttttatat gtaaaggctg catggttact
     2521 tcgctatatt tatttagtaa caggagtcag tgcttcgata cccacactaa ttttcaatgc
     2581 tcggtcaact tctgccataa tgggtcgatc caaatggcag accttttctt tcaatcttga
     2641 tttatcgatc gttctgatct gctccaacag gataacggag tctctttcta ttcctgttcc
     2701 agtagctttg atcccaatgt gagtcggaag cttaggttta gccatttttg ccgttatggc
     2761 cgcaacaatg atcgtcgggc taaaatggtt acctagatta ttttgaataa ccaaaactgg
     2821 gcgtgttccg ccttgctccg atcccacgac tggagaaaga tcagcaaaat aaatatctcc
     2881 tctttttacc ataggatcct ccttattctt gatattctct tggtatccgc tgtcctaatc
     2941 cgcaagccac ttcatagtgg atcgtaccta attgatctgc tatatcctgc atagtgattt
     3001 ctttgtctgc atttttgccg ataagcgtca cttttgttcc gacaggaaat tcttgaggca
     3061 atcggatcat cagttgatcc atacatacac gcccaatcgt ttcacagtag ttcccttcta
     3121 ccaatagaga aaaaccctgc atctttcgtg gccagccatc tgcataaccg atcggtatcg
     3181 ttccgatcca ctctgcttca ggagtgatat atgtttctcc atagccgatt ccttcacctg
     3241 ctgggagttt tttgacttga atcaattcag aaaccagctc aagtgctggc tgcaatggat
     3301 aaacttccgg tagtgcgtgc ccagagggat taagtccgta cattgctact ccataacgaa
     3361 tcatattgcc gattgtttca tcatgccata aagccgttgc actattgctg acatgtacat
     3421 atcgaggaag ttcgggaagt tttttcagca cttctataaa gcgttctttt tgcagattcc
     3481 aatatgtatc atctgcctga tctgctgtag aaaaatgggt aaataaacct tcccattcta
     3541 atacacgact ttctttgatg aatgcagctg cctcttttac ttcttcaggt gtacaaaatc
     3601 cgatacgacc catcccagtg tctgctttta gatgaatcga aagaggcgtc tctagcttac
     3661 ttttctctaa aacagcagct gctgcttgca accattcccg tgtacctgca gttactgata
     3721 aatcatgagc aacagctaca gtcagtgcgt cgtaaggaac catattcaag ataaggatgg
     3781 gctgagtaaa tccagcttct ctcaactcga ctccttcatc taaatttgaa acacaaaagc
     3841 ccgaagctcc acctgcaact gctgcctcag ctgtttcaat agctccatga ccgtatccgt
     3901 tcgcttttac tactgcaaac aattcttttc cttgcggaag tcttgcaact tcatttgcta
     3961 cattttcagt tatagctttt ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa
     4021 ccatcgtttt tccttctttc tagtcttcca aaataatttg tgcaaaagct gtttcatttg
     4081 tatgagtaat actgacccat acattgcctt catgaggtga acgcgtcaca acaggctgac
     4141 cattttcatt ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc ccagttcccc
     4201 aagctttaga gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct acttggcgat
     4261 gaaaaggcaa ggactggaat agtttcattt catcagaggt caagattcgt gcaatgaatt
     4321 ttggtttttc ttcgatcagc cgagtgatcc taggaagttc gactgcatcg atcccaattc
     4381 cttttatcat atgcttttct cttttcaata aatttagaac actctcattc taccaaatta
     4441 taaccatttt gccacaaata agtccacaaa taacaaaaat gaaagtttct cttaaaaaag
     4501 agaaactttc attttatgtt taatcgttat ttgaacggat tacaaatccg cgttttttat
     4561 cattgtgacg tttaccgcca tctttttggc tgttattatt tttgctataa ccgccttttg
     4621 atttgtttcc acgataatta ccgccgtcac gacgattacg gttgtttccg ccaccacgtt
     4681 tgccattttt attgtatcct tttttctgca ttggcaatgg gcgttctggt gtgatcttca
     4741 ctggcactgc atcagctgga tcttttgctg tcgtttttag caaaagtgca actaaatctt
     4801 gtgcagaata ttcttctaat aatttatctg ctgtttgcag gtatttatct agaccatttt
     4861 ctgcaagttt tgtttcgatt tgttcaactg cagcacctaa ttgacctttg aatgcttctt
     4921 tttcggttgg tggacgtaaa gtcgtcatcc gttttttcgt caagttttca atcacatgca
     4981 agtagtccat ttcatttggt gtaacgaaag tcacagacat accgcctttt ccggcacggc
     5041 ctgtacgtcc gatacggtga acgtagcttt ctggatcttg agggatatca tagttgtaca
     5101 catgagtgac acctgaaata tctaatccac gtgcagcaac gtctgtcgct acaaggatat
     5161 ctaaatgacc gctcttaaat gaacgcaata cgctcattcg tttttgttga gacaaatcgc
     5221 catggattcc ttctgcacgg taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac
     5281 ggcgttttgt acgtccgaag acgattgtta attcaggtgt ttgaacatca aacaagcgag
     5341 tcatgatatc gaatttttcg tattcttttg cgcggacata gtattgatcg atcaagtcag
     5401 ctgtcatttc tttcgctttg atcttcacat gatgtggatt tttcatgaat ttaacgccaa
     5461 tattcttgat agctggcggc atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa
     5521 cttgtgaaat gattttttcg atatcttcca agaagcccat attcaacatt tcatctgctt
     5581 catccaaaac taatgtttga acggttccaa gtttcaatgt gtggcggtta atgtggtcaa
     5641 gcatacgtcc tggtgtaccc acaacgatat gtggacgatc tttcaagcca cggatctgac
     5701 ggccaatatc tgctccgccg taaacggctt gtacacgtac ttttttgtcg cgccctaaac
     5761 gatataattc ttcttgtgtt tggatagcta attcacgtgt tggtgcgata actaaacctt
     5821 gtaattcgtg acgatctgga tcgatttttt ctaacattgg taatccaaat gcagctgttt
     5881 tccctgtacc tgtctgtgct tgtccaataa cgtcttttcc agctaatgct agtggaatcg
     5941 tttctgcttg gataggcgtt gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt
     6001 ctggtgataa ttctagttct ttaaatttca aatgaatcct cctatatggt tgttaactga
     6061 ggatgtagct tttttgctac cccgtcgatt tgttcggtca aagtgtattg tttctttttg
     6121 acagtggcca cgcacgtttt tatccattct tagcccctgt ggcaccaccg aaaaatcact
     6181 ttgcgaagtt taggcattag aaaagccaaa cgggtcgcgt tcagctttat cattattatg
     6241 acatctggct tcaacaagtt gtttctatgg caaaaccagt ccaatttctc ttcatctttc
     6301 ggcaataggt tattctaaca tagtttttcg ttttctgcaa gtttacagat ttttatttca
     6361 tttgctgaag ttgttctacc acttcaaata agcccatgct gttgctgcct tttagcataa
     6421 tactatctgt tgctgcaaga ttttgtttga tagcatcaat caatttattc ttttcttttt
     6481 caaaatagaa cacaggtagt tgggggtatt tttggttcaa ttcttctttt agtgcatgca
     6541 tttgttcccc ataaaggaaa atcatggcga atttctcagg atcgatatgt tcactcattt
     6601 gcgcatgcat atttaaagac tctttcccta attccagcat atctgctaac acagcgattc
     6661 tgcgtccttt tgttggcaga tttttaaaag tatctaatac cagtcccatg gctgttggat
     6721 ttgcattgta gacatcgctc aaaatagctg caccatttcc tgccgttagc cattcggttc
     6781 tgttttgagt gatagaaaca tgggcgagcc cttggcgtat ctcaggtatg gacagtccaa
     6841 agaagcgccc gatcgtatac gcaatcaaag cattcgttac attataactt ccaggcagcg
     6901 ggatcgtaaa tgtttctcct tctactacaa aatcggtttg tgcttttcct tttgctgtca
     6961 cttccgcatg aacatcgcca tttccaatac cgaatgttgt gactgtttgt actaatgttg
     7021 ccgttaaagg tgtcagcaac ggttcatctg cgggcacaac gagtaatccg tctgatacaa
     7081 gtccgtcagt gatttccatt ttggctttag cgattccttc tcgcgaccca agattttcta
     7141 tgtgtgcttc accgatcatt gtgattgctg cagcttttgg ttgagccata agtgaaagtt
     7201 ccgtgatttc attggcgtga tccatgccca tttccaaaac gatcttttct gtatcttggg
     7261 gcatgtgcaa aatcgtgtaa ggaagaccaa tattattgtt gtaatttcct tgcgtcttat
     7321 atgttttgta tttttcagac aagacagacg cggttagatc tttcgtggtg gttttaccat
     7381 tgcttcctgt aattgcaacg acttctgcgc ctactttttc caagtaatag gttgccaatt
     7441 gctgcatggc tttcaaagga tctgctacag gaataactgg aaaatttttt ggagcttgtt
     7501 caacaggcaa gctccaaaat gaagcagatg ctccgtttgt gatcgcttgc tggataaatt
     7561 catgtccatc tcgcgtacca gcaagaggaa cgaataaact tccttcggtt atcttccggc
     7621 tgtcaaattc gacattggta atctttgtca gtgcagagca ttcgctgccc acggcttgtg
     7681 caatttcttg tattgttaga ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg
     7741 tttggcctaa acggcgggtt cgcatgactc tcacttccaa gtaaatttct ctattcgtat
     7801 ttagaagcta gaatgaacgc ggttacgcaa tcactccagc ctcttcttta tttctcttta
     7861 ttcatttttt tcaaaaaaag attgacgctg atggtatcga ttcattccta actggatcag
     7921 ttcttcaatc aaatcaccgt atttcaagcc catattttcc cataagagtg ggtacatact
     7981 gaactccgta aatcctggca tagagtttaa ttcattcagg aataattcat ttttatttgt
     8041 caaaaagaaa tcgcaccggc tcaatccgct tccacctaac atcgtgtaag ctaacttcgc
     8101 gtactcttgc gctttttgat aaacttcttc tggcacttcg gctggaatct gcatttcgat
     8161 tttattattg atatattttg cttcataatc atagaatgct acgtctttta cgacttcacc
     8221 aggcaaagtc gtccgaacgt cttcatttcc taatacagca acttcgattt cgcgcgcttc
     8281 aattccttgt tcaacgattg ctcgagaatc atactgatag gctgttgcta aagcattttg
     8341 cagctcttct cggttttctg cttttgtaat gccgacacta gaacccatat tcgccggttt
     8401 gacaaacatc ggataaagca aagaaccttc acattgatca aatacttttt taggattttc
     8461 tttccattga ttcttaagta ctggtacata aggaacttgc ggcacaccag cagcttgtaa
     8521 aatatacttg gtcatgattt tatccatggc acatgcactg gtcaatacgc ctgcgccgac
     8581 ataaggcata ttcaatgtct ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc
     8641 atgtaaaact ggaaaaacga tggctccttc ttctttgatt tcgcccggat tgatcacttt
     8701 tcctgtaaag ccttcctctg tctgtccact tgggtcccat gaaagatgca agacatcttt
     8761 gctggcaggt ttttctgtta gtaatggacc tttgacccat tgtccttctt ttgtaataaa
     8821 tacgagttga acttggtaat aattataata aatggcattt aaaactgaaa atgcggaaag
     8881 aatggacact tcatgctctg cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt
     8941 gtcctcctgt attctaaatc gttcagtgat ttactctcat attttagcac aaaacaaaaa
     9001 taaacgaata acttatttat aaaaaaacga gccagcttct aaaagaacga gcgtcattta
     9061 ttttttgtca gctagattat ttgtcccgtt ttttataaaa agttatgata aagaaaagga
     9121 gggatgatga tggcgcaaac aaatccacgc agccgcttgg cagaaatcat tcgagtgttc
     9181 atcaattaca atgtagtgcc aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa
     9241 gcctttgaag aactaggccc caccttcatc aagataggac agatgctttc tgttcgagaa
     9301 gatttgcttt cttctgcctt cactcaaaca ttcaaaacat tacaagatag tgtacctagc
     9361 gacacatttt caacggtcaa aaagacgatc gaaacagaac tttctctttc attatcagat
     9421 atattcgatg atttttcaaa atcgcctttt gcctctgctt ctatggggca ggctcatcgt
     9481 gccaagctga agaacggtga cgcggtagtc gttaagatcc aacaccctaa catcgccgaa
     9541 gagatccgac ttgatcttca actatttgaa cgagcgatcc ctttgatcaa atacattcct
     9601 gaaactagcg tagttgatct aaaaggggta ctccaagaag taaaacgttc cttgatcaat
     9661 gaaatggact tcttaaaaga aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc
     9721 tggaaagaga tccgcagccc caagatttat gacgcttttt gttcgaaaaa agtgatcgtg
     9781 atggaagaaa tgtctggtaa aaacttgaat catttaatga acgccgaaaa caaaactgaa
     9841 acttttatca ctggaattca aaacaagcag ctgaaacaag aagttgcaaa gcttttagtt
     9901 gagaacttta tgaaacaggt gtttgatgat ggttttttcc atgctgatcc tcatccaggg
     9961 aatctgctat tccatgtact aacaaaagaa gaacagacac aagctagccg aaaaactgaa
    10021 acagtccatg aaaaagagtt cggttccttt gcttttcgag cctccacttc tgcagaagac
    10081 cctgttgcgc catataccat caactacatc gattttggta tgatggggca tctttctgca
    10141 ggacttaggc aaaaactcac acaagccgtt ttagcgctgt atacgaaaga tgcttatcga
    10201 atcgaaaaag cggtccttcg actttgtcag caagaaggct cgtttgatga aagtcggttc
    10261 caccaagaac tgacaagttt tcttgaacag tattatgatt ctcctattga tgaaatcaac
    10321 ttgcaagaag tattcacaca tgtcgtgacg atttgtcatc aaaataatct gcagttcgat
    10381 cgtgatatca cgcttttgct gaaagcattt gggacattag aaggggtgat ccgcgtcctc
    10441 gatcctgaag tttccctcat ggaagtcgct tcgccttttg ctcaacatta ttttctcact
    10501 catctggatg tagaagatac attgaaacaa agcggattag acttattgga agggatgaag
    10561 gctgcgccta agatccctca acagctccac catctattag aaatgtggac ttcgggacaa
    10621 ggaaaagtca atttggaatt gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg
    10681 atcaatcggc ttgttttcgg tatgatttta gctgcattga tcgtagggtc ctccctgctt
    10741 gtccaagcag cacctgtaga aaacgcagag gtcgtttcct tattagggat atttacgtat
    10801 gctattgccg cattcgtcat tatttttttg gcaatcgatg cattgatcca aatgtataag
    10861 aagaggaaaa agtagaaaaa ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg
    10921 gttttacata aattgtttct caaatttttg tgaaaattcg gcttattttt cgaagaggct
    10981 acttctgaaa caccgagaat aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa
    11041 caatcaaaaa gggaacatgg aacacattta tcctgtttcc ctgttccctt tttgatgtat
    11101 tcgagcataa ataaaatacc ttcgttacct actatcttct gtacttggta aaccttagtc
    11161 aattgccttt tttaacgctt gacgaaaaga catatgatct ttctccgtaa agggttttgg
    11221 accctttgtc cttatgccag cttttcttaa ctttccacta tgataacgtt gttctagaag
    11281 cgtgccgata ttcgcttcgt caaattcttt tccagagtga tggaacactc gcacaccctt
    11341 cggcaataca agtgaagcta aaccataatc ttgcgtgatc aaaaagtcat tcttcttaac
    11401 cataccgacg attttataat ccgcactatc agctccgcgg tctacgtaga cgatttggac
    11461 atgattagcg tattctttgg tcgtataatg gtccacactg gtcacgataa ccacgtcaag
    11521 accataggat tctcctaatt ttatgacatc ttcttttact ggagaaccat ctccatcaat
    11581 gaatagcctc attctttttc gccttcttcc ataaatgata aacagttact aatccgctca
    11641 gtaaaagacc aacattgatc cattttgcca agataagcag ttgtacactt tctatcggca
    11701 ctgattcatg gttcagcgtg atcaataata gatggattgg ctctcccatt tcttttgcta
    11761 aaacaaacaa aagcgtcatc aataaaaact ggtggatcac actcccttta ttttgagtag
    11821 tatacttgac aacgatccgg cgatccattg gaatagctgt atcagtttga taatattcct
    11881 tatttagcca gctgcctgtt actacgtgcc agaaactaag cagaaagaca cctatcgcaa
    11941 aaccatttgg aatcaagagg accaataaag aagaactcag tccaagcaat tgcatagcca
    12001 atggagacca tgttaaacgt ttcaaaatcc atggtccgac tatttttgct gcaatcaagc
    12061 ctgctgcata aggcaaatac ataaaaatcg aaagtttttc taatccgtac agtatgccaa
    12121 cataaaaact gctaaataaa aaaaggaaat tccctagcat tccatttaga aaagtaaaca
    12181 gatttagcca gcttggcaac ttccattcct tcttctggtg tcctaaaaca aacacgaaca
    12241 agatgaacag cagcaagaaa ccataaatag caagatcaaa aagcctttca tcgaataaca
    12301 atcgggcatt tctcaaaaga aacaataagc caaagaatat caaaaataaa acaagctctc
    12361 gcgtagctac cagatttctt ttgatgtctt tgaaatccag ttcgtaacgc ggataatgac
    12421 taaccgtatg gtaggccatg acgtaaaaaa gtgtataaat agtaaagctt gctgcaatct
    12481 gagtcggtgc aggcaactca atcgcttgat acaggagaaa tagccagata gctgcaaata
    12541 agtacttttt cccatccata ttgataaaac cttgcgtctt ttcgtgaaaa ttgaccgtgg
    12601 tattagcagg tacaagccat gctgcactca gccctagcaa aacacttgaa aaataaaata
    12661 aaggaaaata aaatacgcct aatattccaa gcaatgaacc agcaccacca atcagcagag
    12721 cgatcatcag caacgtgtaa ctatctagac caaatcgtag actgttgact aaaaatagtc
    12781 cagtcatccg aaaggcataa aacagcgtaa atggcaaaat cgtcagccaa ttatgcgagt
    12841 ctgttagtcc caaaaataaa agataaggaa taaaaataac cgtgttactt aagataaagg
    12901 gcgttgtttg tgtaaaatta cgcccaaatc gtttgagttt ttcgaacatg acaatggctc
    12961 cttaaacagt cttataacta atctgctctt cgattttagc attaaagggg aaataaacca
    13021 attttacaga atatattctt gtaaaaaaaa atacagaaag aagcataagc ttctttctgc
    13081 atttttacgg tacttgatta ctcaagcaga caagcccttc tccacaaaag tcttgcccga
    13141 cacccctgca tccggcaggt gataaagata gaaatcaatt atcctgtgcc tccaaaatca
    13201 attaggagct ctgtaaacca gagtccttct cgacccgtcg cataaattta ctttaacaca
    13261 gttcctatgt ttatgcgaga gcttttttca ttttataatc gaccatttga aatccttgac
    13321 cgatatctga aataagttct tctgtaatct caaatccttc atgcttataa acctcgattg
    13381 cttgatgatt cccctgattc actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa
    13441 gttcttcata ccagcgaaaa actgaagaag tcaatccttg accacgtact ttatttagca
    13501 aatataattt acttaaataa atttttccat ttatctcttc ataagcagtg taaccgacag
    13561 tcttttcttc gctttttaat aaaaagtagg acacaccttc tgtcagttct ttttgaatct
    13621 gtttttttga ctgataagtt ttcagcatgt aatccacttg tttttgacca atgatcggca
    13681 caaaagcttc cggccatact tcttgtatga ctgccatcag ctcatccagt tcattttctg
    13741 ttacttttat tttttccata cacccacgtc cttttttaca ttataaatga ccttgcgctt
    13801 gatggaataa aaagagcatg atcatgctat acacacccac cgaccatggt ttcaagaaaa
    13861 tcacaatctg cataaattcc ctaaaagata atttcgtcaa accagctact agacagatca
    13921 agtcgtctgg cgcaaaggga agcagcaagg taacaactag tactttttta atatctttcg
    13981 tttttcttgt cagcaattca tcaaatttct gatacttttt tggcgataaa ataagctgga
    14041 caaaggctac gccatagtaa cggacaagca aaaatccgat tgcttcccca atcactaggc
    14101 caatacaact atataaaagc ccaaatccat tgccaaacat cagcatgccc gctactgagg
    14161 agatccctcc cggcaaaata ggaatgatca cttggatcac ttgcaacaga acaaatatca
    14221 tcactgcata cttgccaaat tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa
    14281 tcccatgatg ataagcataa agaatcaaca caaagaaaaa aaacagacca ataatgggca
    14341 tgatctgaag acatttttgt aaaactgatc cttttttaga catctatatt ccccacatct
    14401 tttttcattt attaaaactg cctccttagg gggtgcgaca aaagagcttc ttcaaaaaaa
    14461 taagtcgaaa tctcacacaa attcgagaaa taattttcgt aagtttcttc ttatttattg
    14521 catctgacca cttctgttac aacctctcgt taaacggtgt tcaaaagctt gcatctacga
    14581 taataagccc aacccaacga aaaacgtgag gagctgtttt atttggatgc tcttatcgct
    14641 tgatgcaaag acagcttttt tacaacctct acttcacggt gctcaatcag ctgaccttcg
    14701 gtattagctc aaaaaacggt aaaatataaa cagctatttt ccgtttcttc tttttaatac
    14761 ttaggtcaag atgctgtttt cacaacctct tgttataaat aaaaaaacag ttggctggtg
    14821 actgttcaaa aagcagaatc cttgcttttc aaacggtcgc agtccgttca actgtttcgc
    14881 tgcgcactat ttatggttta agaaactagc tacttttgtt gtaatcaaat caatagcaac
    14941 gtggttttct ccgccttctg gaacaatgat atccgcatag cgcttcgttg gttcgataaa
    15001 ttgatgatac atcggtttaa caactgtcaa atattgttcg atcacggagt ccaatgtgcg
    15061 tccccgttct tccatatccc gtttgatacg acgaatgata cgaatgtcat catctgtatc
    15121 tacgtatact ttgatatcca tcaagtcacg cagacgttcg tcttctaaaa tcaggattcc
    15181 ttctagaatg atcacttctt ttggttcctg aatgatcgtt gcctgacttc ttgtatgcgc
    15241 tacatagtca tacacgggtt tttcaatggc tttatagttc agtaagtcgc ctacatgctg
    15301 aatcagcaga tcattatcaa aggcaaatgg gtgatcgtaa tttgtgttca atcgttcttc
    15361 aaaactcaaa tggctctgat ccttgtaata agaatcttgt tcaagcatca tgatggaatg
    15421 gtccgggaag ttgttgaaaa tcgcacggct gacacttgtc tttccgcttc cggatccacc
    15481 cgtcacacca atgataattg gcttgctttt tgtcattgac taaaacctct ttcgtatccg
    15541 ttttttcatt ctaatctatt atagcgaaaa aacacgaaaa tgctatattt ttctgaaaat
    15601 taatcctttt caatttccaa taatgaataa agttcctcta atgaatcgat tcgataagta
    15661 ggctggattt cttcgatttc tggtaattta tttctattga accaaatcgt atcgatccct
    15721 gccatatttc ctcctttgat atcagaagtc aaagaatcac caatgatcac agtttgcttt
    15781 ttatcaaaat taggaatgtg ggaaaagaca tgatcaaaaa attctttcat cggcttttgc
    15841 gctccgacag cttctgagac aaagatatcc ttgaagtaat caagcattcc tgcatctgtt
    15901 aatcgccgat gttgcgtgtg cgacacaccg ttcgtcacaa tataaagatc agcatgcgga
    15961 tggacctttt tcaggatttc tagacttcct ggaacaagat catgaccttg gttcaagtag
    16021 tggcggtagc gtttttccat ttgatcacta tctactgttt tcccaaattg ctggaaaagt
    16081 aacccaaaac gactgcctat tatttcttct cgtgatagtc tgcctttttc aaactcctgc
    16141 cataaatgat gattcaaagc cttatagctg gtttcgattt ctggtgtcaa tgtgattcct
    16201 tgttctgcaa acaaagcact taaagcattg gcttcagtta attgaaaatc taacaatgta
    16261 tcatctacat cgaacaataa agttttatat ttcatctttt tgacctccta tttaaaataa
    16321 atacttaact ttttcatctc tgctaatgtc aacgatcgat actcgcctac tcctagctgt
    16381 tcatctaatt ccaatggtcc catgcttagc cgtttcaatg cggtcacttt ctttccttgg
    16441 gctaagaaca tctttttgac ttgatggaat tttccttctt ggatcgtcaa taaaaccgta
    16501 ctttggtgct tgtctgcttt gagtattttt aaatcagccg gcttacattg atacccatca
    16561 gcgaatacga ttccattttc aaatgctgtt acgtctgccg gtgtcaccct ttcgttcaca
    16621 actacttgat aagtctttga aacctttttt tctggaagta gcatttcata tcccaattgt
    16681 ccattcgaag tgatcagcaa tagtcctgtt gtatctctat ctagtcttcc cactggatat
    16741 aatggttgga aacgatcggc acttgcaatc agatcgatta ctgtcgtctt ttctggatct
    16801 gatacagctg taacagcttt ttcaggttta ttgagaagat aataggactc atttgtttct
    16861 aagtgttcac ctgctacttc gatttgatgc agtaaactat ctacatttcg attttgtcga
    16921 aattcgactg tgccatctat catcacttgc ttcaaagcaa acagccgctt catttccttg
    16981 cgtgtcgttt ctaatttttc ttctattaat ttatctaaac gcatgctgtt tcctttctta
    17041 ttactactaa ttaattatac cttgaatatt cttgcttgca aatacatcat catagttatt
    17101 ccttgactta attaacagtt cacgttacgg ttaaatagta aagtgaattg gatttctcat
    17161 acacagcgta atgacctggt ggagaaaagt tgaatcctga ctttctaagg ggagatgcgc
    17221 gcatctctcc ttttgcgctt ttttaagcgc cctgccttcg atgatgtgca cgagattagc
    17281 ctttagaatt cacttaaata aaatctgtcc gttaccaaag cagacagtca cattttaagg
    17341 aggaattttg gttatgtcag tatcattaga agtaagtaaa agagaagtac gtccccgttc
    17401 attaagaaac aaacttcgtc acgaagggaa agttccagct gttgtttatg ggtataaagt
    17461 agaaagtacc cctatctcat tcgatggttt gacactaagc aaattgttgc gtgaacatgg
    17521 agcgaacaca gtcatcacga tggacatcga tggacaaaaa gtcaatacat tggttcacaa
    17581 gatccaaacg aacactttta ccaatcattt cgaacatgca gaatttctat ctgtaaacat
    17641 gtcggaagaa acagaagtcg aaacagaagt cgttcttgtt ggcgaatcag tgggtgctaa
    17701 atctggtgga gtgttagctc aaaacttgta tacagtagtc gtatctgcta caccagacaa
    17761 attgcctgaa cgaattgaag ttgacgtgac aaacttggaa ttaggtgatt ctattacagt
    17821 agccgatctt ccaaaaaacg acgattacaa attcgtgacc gacagtgaag agcaaatcgc
    17881 tgctgtagtc gctcaaagtg aagaagaaag cacaggtgaa ggtggcgaac cagaagtgat
    17941 cggttcaaca gaagaataat actgaaacaa tcaggtggtg ttagaagcgg cgggtcagca
    18001 gcttttaaca cgcctttttt atttctaatc gtttattttc atatggggct tccacttttg
    18061 taaatggctt tacaaacgaa aaatcctaat ttaccaagct tttcctcgca tacttttatc
    18121 gtacctttat aaaaatttat actttgaaaa aaagtattca attattgttg catttttcct
    18181 gattctttat tagaatgaac cctcgttgaa gtaagtattt ctagtttatc ctaggattaa
    18241 ttataaaaat aaaacattcg ttgattcgaa ggaggttaaa cagatgtttg aaagttttga
    18301 tagcagccgc aaccgttacg cttcagttgg tgtcgtatca agtttgccag atgaattgat
    18361 tgacagtata tggtttatta ttgacctaga tcttaaggga gtcattccat tagaaaatat
    18421 attaaccttt gatttgatcg aaaatcgtgg aaaagtgacg atgcattttt ctcaagcagg
    18481 aagcaatgtg gaaatggata ttgatttgcc gtttgcctat tcgaaccaat ttcctgctca
    18541 agtatacgcc tatgatgacg gtacgagaga aacgatcctt ttaccaagtg agattagaca
    18601 atattaaggt gatagataaa cgtggatgtc ccttatttta cagaccaggc caagttgttg
    18661 attctttgac tcaattcttg gcttcttttt atggagtttc ataaattatc attgatttct
    18721 aattggtatt ctcaggataa acgctatata ctactaacta aattttaaag atataaggag
    18781 tttaaaagat gcgcgattta acaaaaggaa gccccgctaa actgattttg atgttcactg
    18841 ttcctttact gatcggaaat gtttttcagc agttctacaa tatgatcgat atgatcatcg
    18901 tgggtcagac gctaggtaaa aatgcgctgg cagcggttgg tgcaactgga agcctgacat
    18961 ttttgatcat tggctttgcc caaggtttaa cagctggttt agccattatt accgcacaac
    19021 gttacggtgc aaaagattat cggggtctga agaaaagttt tgctgctagt gttgtcatta
    19081 gtcttgtcgt cactgtgatt ttaactgtcc taagtctcct gtttattcgt ccgatgcttc
    19141 aattgatgca aactcctcct gaaatacttg atcaagcaca aacatttatt tcaattattt
    19201 tattagggat ctttgcatca gtcagtttca atttgctttc taatgtcatt cgcgcacttg
    19261 gtgacagccg cacaccacta ttctttctga tcattgcggt catcatcaac gtcgtacttg
    19321 atttgatctt tattatctat tttggcatgg gtgtggaagg tgcagctatt gctacagtaa
    19381 tcgcacaagt ttcttccagc gtactgtgtc tagtgtatat caagaaaaaa atgcctttgt
    19441 tacagttacg caaaaaagat ttcaaatttg aaaaagaaga gtttgctgta cacctaaatg
    19501 ctgctttgcc aatggcgttt caatcttcga tcatcgctgt tggtgctatc gtgttacaag
    19561 cagcactaaa tagtctaggg acagatgtcg tagctgctca agccgctgct ggtcgtatcg
    19621 accaatttgc aaatcagccg atgatgtcat ttggtattgc aatggcaact ttcactgcac
    19681 aaaactatgg tgcgaaagaa tatggccgaa tcttgcaagg ggtacgtcaa actttactga
    19741 tgagtgtcgg atttagtctc ttagcaggtg ctatcgtcat tttcttcggt catttctttg
    19801 ttcaattgtt tgttagccca tcagaaacac gtgtatttga attagcacaa acctacttca
    19861 atatcaatgg gtctctttac tggatcttag cagtcctgtt cattttacgc tacacacttc
    19921 aagggcttgg acaaagcaag atccctacga tcgccggtat gatggaattg cttatgcgtt
    19981 cttttgcagc tatcgttctt acaggaatgt ggggataccc aggagcagca gcagcttctc
    20041 ctttagcttg gatcggttct gttgccgtgt tgctttactc ttatatgcgt tcgatgaaac
    20101 aattgaaaca catgggtgaa gaggaacatt tcacattaga acatgcagaa gcaagatagc
    20161 cttgattaaa agaaatcgtt gcggctaaaa atgaaaactt ttattgaaaa tatcttcttc
    20221 gtttaccgaa aacaggtttt ttagataaca aaaaggctgt gacaaaaaat gtcacagcct
    20281 tttttctttt attctatttt tctttccgtg gactctactt tgaaataatc gatatgtgca
    20341 tgctggtatt ctaataccgt gaaacggaat gatgcaatct caatgattgt tcctgctttt
    20401 atttctttat atcggctaga agcaaagccc gcaagtgtag taaaattcgt ttcttcaaac
    20461 tctggaatct ctacatgaaa ataacgctgg aaatcatcta atgttagttt gccggagatg
    20521 atataagagc catctgactg ttccgctatc aatgcttcgt ccaaatctgt ctcatctcgg
    20581 atttccccga cgatttcttc taaaatatct tcaatcgtca ctaagccgct tgtccctccg
    20641 tattcatctt ttaagatagc aatatgcttt cccttttttc gcatgatcgt caataatttt
    20701 tttataggga tcgtttcagt tgttacgatc ggctcttcaa tcaactgttc tactgtcagc
    20761 tggtcatctt tataagattg cttgatcaaa tctggcaagg tgacataacc taaaacattg
    20821 tcttttgatt ctaagataac cggatagcga gtatgtcctt gttcaatcga agcttttatc
    20881 gcttctttga cggtttctga agcttcgaat acttgcatgg acgtacggtc tacttgtata
    20941 tcttttgcca aggtatcatc aaattcaaac acgttttcca aataatgata ttcttctttg
    21001 gtcaactctc ctttttcata agaattcaat gcgactaatg tcagctcttc ttgagacaat
    21061 gtctcttctc cttcagatac gagacgcaca cctaatagct tccctagccc tgcagctgaa
    21121 tgattcaata cccaaataaa aggaaacatt actttataaa aataatgaag tggtttaact
    21181 acgaacaaga cgaccttctc cgtattgacg atactatagc tcttaggaag cagttcgcca
    21241 ataaccacat cgatatacgt aactaaaata aatcccagcg ccacagagat tgtactgcta
    21301 atagccgaac taaaaggtag tttaccgatc actggttcta gtaaagccgc gacagatcct
    21361 tcaccgaccc acccgatgat caaaccggtt agcgtaattc ctaattgact agctgataaa
    21421 tagttatcta aatggtgtgt aacatgaaga gctagcttgg cgttctttac attttccttt
    21481 tctagttgtt ccaatcttga tcgtctgatt ttcactaaag caaattcact tgctacaaaa
    21541 aatgaagtaa ttgcaatcat caaaaataaa ataatgaaat tgataaccat attctcccgc
    21601 ctttctcttt ttctattgta ccctttttct acaaaaagat tgaaaataat gcgtattcat
    21661 atttaggaat aaaaaaggag atgcggcatt tttcagccac atctccttat cacttcaaaa
    21721 tcattgcgtt acttgttgct aaacagcttt ttcatgacct ctgataaacg gtgttcaagt
    21781 agctgacatt cgacattagt tcaggtcaaa gcgctacttt cacaaccgct ctttcaacca
    21841 tgttttccga gtatttccaa tgataagcgg aacagtttca tgttttactt cgctgtactc
    21901 tagcccaaac catgtttctg gcgaggtagc gatttttttg attgccagtt ttgcctgcat
    21961 gatttgacgt tgccattttc ctagagttgt cacattggaa actacttcat caatcaggac
    22021 aaattggaaa tcgccaactt ctcgccctgg tgtcagagag tatctttgtg gctgttttgg
    22081 caaacggcct tgtttcatca aatcatggat gacttggcga acatatacgt tgatttcctg
    22141 ttggaccctg aaacctaaga acagattcac ttggacaata aaatcagtgt ccatcatatc
    22201 tacttgatat tctttcgtat atggctcatc cgtcacttcc acatgaacaa accaatagac
    22261 tcgtgctctt ttaggccgtt tatctaaaat cgaatagatg aactgtcggc caactttatc
    22321 ttcagccata tcaggaacca agaagaccac atttgtctga tagattggta aagaagtatc
    22381 atcacgtaat tctctaagct gcgggatata atctctcaaa gacacatcct cggttgacga
    22441 ttcgcgaata atgttccctt gttcccaaat aaacatcacg actaagatga tcaaaccaat
    22501 cagaacggct acaaaaccgc ctcggaagaa tttgacaaca cttgagataa agaaaacgac
    22561 ttctatcgca ccgaagaaaa aggtaactaa atatgcaagc caacgagaat gcccattttg
    22621 gataagataa aagtaaagca gtgctgttgt catcaacatc gtcacagtga tcgatagacc
    22681 gtaagcagct tccatatgat gagacgttcg gaatgtgaca accactagag aacaggcgat
    22741 ccataaaatc agattgactg ctgggatata catttgcccg atactgttcc caggatacat
    22801 gatctttaac cgaggcagca gtttcaactt gattgcttct gaaactagcg taaacgaacc
    22861 tgtaatcaat gcttgcgaag caatgactgc agcgactgtt gcaaaagcaa cgccgatcac
    22921 gatccaactt tgaggcatca tttggaagaa tggattcagg ttcttgattt gctgtgcttc
    22981 agggttttga taaacattca acaaccacgc agcctgccca aaataattga taaccaaaca
    23041 aattttgacg taaggccagc tcagacgaat atttcgtttg ccgacatgtc caagatcgga
    23101 ataaagcgcc tctgcacctg tcgtagccag gaaaatattt ccaagaatga acaagcctaa
    23161 tttgttatct gcactaaaaa gcagctggat agcataataa ggattcagtg cacggataac
    23221 tgtccagtct tgactgaagt tcattaatcc cattacgcct aaaaaagtaa accaagcgaa
    23281 catgattggt ccgaatgctt ttccgacaat atctgtacca aagcgttgga ccatgaataa
    23341 aacaaaaata ataactaaag tgataatgac gatcacgttc tggttattac cgaaccggtc
    23401 aaaaaactgc ggaattcctc gcaagccttc aatcgcagtc gttaccgtca cagcaggggt
    23461 caatacccca tctgctagta aggcagctcc tccgatcatt gcaggaatga tcaaatattt
    23521 cccacctttt cggacaagtg tgtagagtga gaaaattcca ccttcaccat ggttatctgc
    23581 attcaatgca atcacgacat atttgattgt tgtcagtaga gtcaatgtcc aaaaaatcaa
    23641 tgagactgat ccgataacaa aatcaggatt gattcctgac aatccgccat tatcttcaac
    23701 aatcgatttc ataacgtaaa gcggacttgt cccgatgtct ccgtagacga ctcccatcgc
    23761 tacaagcagg ccagccattg atatcttttt ggtattgtgc gttgttgcca cgtttttccc
    23821 tcatttcttg ttatatggat atcctttagg taagcttgaa tgatagatga tattcttatc
    23881 catgacaccc gctttcctaa attgcttcta attatccaag taacacactg aagtgtcaac
    23941 ttatttgaat aaaattattc tttctttgga cgttctctga tacgtcttgc cacaaatgaa
    24001 taaagagcaa ggcaaaaaat tgcacccaat aatgctggta tcaaataaaa accgccgagt
    24061 tccggtccca gtggtcctag taaaaattcg ccgagccaac tacctaaaaa accgataatg
    24121 atatttccag atacgcctcc cgggacttcc tttcctaaaa ttgcgccagc tatcaaacct
    24181 aatataccgc cgataaataa cgaccataaa aaattcagca tctcctttcc ctcctttatc
    24241 catatttctc acttttattt tacgctttat tcagaaggac tccaaaaata tgcgccaaga
    24301 aactccagaa aaaaagaagt tgtaacaaaa gtgttcagct tatttccgta gaagctgctt
    24361 ctggaacacc gtgaatcaga ggttgtgaaa tcagcg
//
LOCUS       DACCQI010000038        23949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCQI010000038 DACCQI010000000
VERSION     DACCQI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.038"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..556)
                     /locus_tag="IVO76_001167"
     CDS             complement(<1..556)
                     /locus_tag="IVO76_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP7429140.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYFVGA"
     gene            complement(572..1051)
                     /locus_tag="IVO76_001168"
     CDS             complement(572..1051)
                     /locus_tag="IVO76_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7429141.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="IVO76_001169"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="IVO76_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="IVO76_001170"
     CDS             complement(2098..3204)
                     /locus_tag="IVO76_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7429142.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="IVO76_001171"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="IVO76_001171"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7429143.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="IVO76_001172"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="IVO76_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7429144.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="IVO76_001173"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="IVO76_001173"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7429145.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="IVO76_001174"
     CDS             8175..8873
                     /locus_tag="IVO76_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7429146.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="IVO76_001175"
     CDS             complement(9041..11680)
                     /locus_tag="IVO76_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7429147.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="IVO76_001176"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="IVO76_001176"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7429148.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..14698
                     /locus_tag="IVO76_001177"
     CDS             13523..14698
                     /locus_tag="IVO76_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7429149.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            14702..15343
                     /locus_tag="IVO76_001178"
     CDS             14702..15343
                     /locus_tag="IVO76_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429150.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            15340..16257
                     /locus_tag="IVO76_001179"
     CDS             15340..16257
                     /locus_tag="IVO76_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7429151.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            16254..16925
                     /locus_tag="IVO76_001180"
     CDS             16254..16925
                     /locus_tag="IVO76_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429152.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(17466..17954)
                     /locus_tag="IVO76_001181"
     CDS             complement(17466..17954)
                     /locus_tag="IVO76_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7429153.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(18155..18421)
                     /locus_tag="IVO76_001182"
     CDS             complement(18155..18421)
                     /locus_tag="IVO76_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7429154.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(18421..18684)
                     /locus_tag="IVO76_001183"
     CDS             complement(18421..18684)
                     /locus_tag="IVO76_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7429155.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(18677..18949)
                     /locus_tag="IVO76_001184"
     CDS             complement(18677..18949)
                     /locus_tag="IVO76_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429156.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            19160..19345
                     /locus_tag="IVO76_001185"
     CDS             19160..19345
                     /locus_tag="IVO76_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7429157.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(19487..21310)
                     /locus_tag="IVO76_001186"
     CDS             complement(19487..21310)
                     /locus_tag="IVO76_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7429158.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(21908..23872)
                     /locus_tag="IVO76_001187"
     CDS             complement(21908..23872)
                     /locus_tag="IVO76_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7429159.1"
                     /translation="MGEKVVALNDKMPELKDQAKIILDLQEKIPEIQNAGKQLAEIDG
                     DFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDLANQTKSGASQLKDAI
                     PSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKKNISNVLNGFIDGISQ
                     QQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQNRLSALNTAVQNEDTK
                     AIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTIGTAQGALSKADQIDF
                     DSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNMDTIVNGINEGADLYN
                     NELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVETAVNAAADLINNDWP
                     NIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQPVELQTNALYPIANNG
                     SASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQFVARMLTFLTMAIAQS
                     LIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALFDNVGKGIAIIILVLS
                     ISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWPNATKAIWIMIGLFVV
                     FGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
BASE COUNT         6803 a         5152 c         4423 g         7571 t
ORIGIN      
        1 aagcgcctac gaaataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaatcat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg
    14161 tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga
    14221 agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga
    14281 agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa
    14341 actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa
    14401 aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga
    14461 cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca
    14521 acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt
    14581 aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga
    14641 tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc
    14701 tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct
    14761 ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt
    14821 actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac
    14881 aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc
    14941 tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg
    15001 gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt
    15061 ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg
    15121 tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg
    15181 tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg
    15241 tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca
    15301 ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg
    15361 accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc
    15421 aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta
    15481 gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac
    15541 aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca
    15601 gcggctcgct acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat
    15661 ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg
    15721 ttcccgtcct atggttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa
    15781 aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt
    15841 gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat
    15901 ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa
    15961 gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat
    16021 ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta
    16081 gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat
    16141 accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa
    16201 ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc
    16261 aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct
    16321 atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag
    16381 cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg
    16441 tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa
    16501 tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct
    16561 taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg
    16621 tcggcaaagg catggggatg actgctagac aacgactatt catggtagag ttaccactct
    16681 ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg
    16741 cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg
    16801 caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca
    16861 tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac
    16921 gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt
    16981 ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt atgggcaaag
    17041 agttgctttt cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt
    17101 tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca
    17161 ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt
    17221 tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc
    17281 atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc
    17341 ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa
    17401 catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac
    17461 gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga
    17521 agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa
    17581 aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc
    17641 acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc
    17701 cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac
    17761 catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt
    17821 ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt
    17881 tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg
    17941 attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct
    18001 aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca
    18061 ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt
    18121 tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat
    18181 aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca
    18241 gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca
    18301 tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt
    18361 gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca
    18421 tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg
    18481 gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct
    18541 ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat
    18601 ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct
    18661 aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta
    18721 cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct
    18781 tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta
    18841 ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag
    18901 caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc
    18961 gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta
    19021 acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct
    19081 ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt
    19141 tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga
    19201 ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc
    19261 tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat
    19321 caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc
    19381 atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag
    19441 cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat
    19501 ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca
    19561 aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca
    19621 acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga
    19681 taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga
    19741 aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa
    19801 catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta
    19861 cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg
    19921 catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa
    19981 aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga
    20041 caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt
    20101 tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag
    20161 caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga
    20221 aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta
    20281 gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg
    20341 caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag
    20401 caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca
    20461 aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca
    20521 cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc
    20581 ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag
    20641 aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg
    20701 gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga
    20761 tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt
    20821 ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga
    20881 cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag
    20941 atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat
    21001 tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga
    21061 tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg
    21121 gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg
    21181 taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg
    21241 ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta
    21301 agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa
    21361 tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag
    21421 aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca
    21481 aaaatctaac atttttttat gggctttttc tttgaattct ataataaaag gatagaactt
    21541 gacttagaaa atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag
    21601 gctgctttct acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg
    21661 ataaacggtg tttaaaagct tgcatctgcg gtaataagct caaccaaatt aaaaacataa
    21721 gaagctgttt ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc
    21781 ttggtacacg gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat
    21841 gaggaactat ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc
    21901 tcttttttta atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt
    21961 cttcgatgaa cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca
    22021 tgatccagat tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca
    22081 agtttaccgc atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga
    22141 cttggatcgg atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga
    22201 tccctttacc gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta
    22261 aggcgatcaa gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta
    22321 agaaaatatt ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca
    22381 gcatacgagc aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat
    22441 agtattctgt cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag
    22501 tataaaacgg ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta
    22561 gctcaacagg ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga
    22621 tgacttcgcc tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga
    22681 taccagcttt gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg
    22741 tttcgacttc tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta
    22801 gtgctggcca atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg
    22861 gtaattcatt gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat
    22921 ttccattcag cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt
    22981 gatatttttc taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg
    23041 aatcaaagtc gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga
    23101 tgacttggtc tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa
    23161 tgatgccatt gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct
    23221 tcgtgtcttc attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta
    23281 gcaagggttt taattgattt agctgatcaa taattggctg taatttcgta tttccagcac
    23341 ttgtttgcaa tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata
    23401 ttccgtcaat aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct
    23461 taccatcttc aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt
    23521 gtccaattga cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca
    23581 gctgagaggc acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt
    23641 tcacatctgg caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat
    23701 cgattccttg gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt
    23761 gcttaccggc attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt
    23821 gatctttcaa ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta
    23881 ctttagggat gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta
    23941 gttgacttt
//
LOCUS       DACCQI010000039        23828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCQI010000039 DACCQI010000000
VERSION     DACCQI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.039"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(53..787)
                     /locus_tag="IVO76_001188"
     CDS             complement(53..787)
                     /locus_tag="IVO76_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7429160.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IVO76_001189"
     CDS             1114..1524
                     /locus_tag="IVO76_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7429161.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IVO76_001190"
     CDS             1517..2218
                     /locus_tag="IVO76_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7429162.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IVO76_001191"
     CDS             2218..3804
                     /locus_tag="IVO76_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7429163.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IVO76_001192"
     CDS             3823..4044
                     /locus_tag="IVO76_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7429164.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IVO76_001193"
     CDS             4041..4802
                     /locus_tag="IVO76_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7429165.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IVO76_001194"
     CDS             5182..5691
                     /locus_tag="IVO76_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7429166.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="IVO76_001195"
     CDS             complement(5766..6305)
                     /locus_tag="IVO76_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7429167.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVO76_001196"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="IVO76_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7429168.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7372..8169
                     /locus_tag="IVO76_001197"
     CDS             7372..8169
                     /locus_tag="IVO76_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7429169.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8197..8832
                     /locus_tag="IVO76_001198"
     CDS             8197..8832
                     /locus_tag="IVO76_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7429170.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="IVO76_001199"
     CDS             8852..9625
                     /locus_tag="IVO76_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7429171.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="IVO76_001200"
     CDS             10033..10830
                     /locus_tag="IVO76_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7429172.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="IVO76_001201"
     CDS             10808..11221
                     /locus_tag="IVO76_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429173.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="IVO76_001202"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="IVO76_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7429174.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="IVO76_001203"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="IVO76_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7429175.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="IVO76_001204"
     CDS             15998..16558
                     /locus_tag="IVO76_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7429176.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVO76_001205"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="IVO76_001205"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7429177.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="IVO76_001206"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="IVO76_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7429178.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="IVO76_001207"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="IVO76_001207"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7429179.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="IVO76_001208"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="IVO76_001208"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7429180.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="IVO76_001209"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="IVO76_001209"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7429181.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="IVO76_001210"
     CDS             21905..22483
                     /locus_tag="IVO76_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7429182.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="IVO76_001211"
     CDS             22535..23017
                     /locus_tag="IVO76_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7429183.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="IVO76_001212"
     CDS             23134..23508
                     /locus_tag="IVO76_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429184.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4285 c         5115 g         6647 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaga
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcacccggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaataa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DACCQI010000040        23466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCQI010000040 DACCQI010000000
VERSION     DACCQI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.040"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(273..2458)
                     /locus_tag="IVO76_001213"
                     /pseudo
     CDS             complement(273..2458)
                     /locus_tag="IVO76_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            2676..3359
                     /locus_tag="IVO76_001214"
     CDS             2676..3359
                     /locus_tag="IVO76_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7429185.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(3440..4159)
                     /locus_tag="IVO76_001215"
     CDS             complement(3440..4159)
                     /locus_tag="IVO76_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7429186.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4471..4677)
                     /locus_tag="IVO76_001216"
     CDS             complement(4471..4677)
                     /locus_tag="IVO76_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429187.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            4921..5523
                     /locus_tag="IVO76_001217"
     CDS             4921..5523
                     /locus_tag="IVO76_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7429188.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            5664..6944
                     /locus_tag="IVO76_001218"
     CDS             5664..6944
                     /locus_tag="IVO76_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7429189.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            6957..7685
                     /locus_tag="IVO76_001219"
     CDS             6957..7685
                     /locus_tag="IVO76_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7429190.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(7754..9277)
                     /locus_tag="IVO76_001220"
     CDS             complement(7754..9277)
                     /locus_tag="IVO76_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7429191.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            9529..10710
                     /locus_tag="IVO76_001221"
     CDS             9529..10710
                     /locus_tag="IVO76_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7429192.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(10770..12398)
                     /gene="treC"
                     /locus_tag="IVO76_001222"
     CDS             complement(10770..12398)
                     /gene="treC"
                     /locus_tag="IVO76_001222"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7429193.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12795..15506)
                     /locus_tag="IVO76_001223"
     CDS             complement(12795..15506)
                     /locus_tag="IVO76_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7429194.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(15516..16217)
                     /locus_tag="IVO76_001224"
     CDS             complement(15516..16217)
                     /locus_tag="IVO76_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429195.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            16403..17005
                     /locus_tag="IVO76_001225"
     CDS             16403..17005
                     /locus_tag="IVO76_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7429196.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            17086..18099
                     /locus_tag="IVO76_001226"
     CDS             17086..18099
                     /locus_tag="IVO76_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7429197.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            18208..19125
                     /gene="rbsK"
                     /locus_tag="IVO76_001227"
     CDS             18208..19125
                     /gene="rbsK"
                     /locus_tag="IVO76_001227"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7429198.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            19153..19548
                     /gene="rbsD"
                     /locus_tag="IVO76_001228"
     CDS             19153..19548
                     /gene="rbsD"
                     /locus_tag="IVO76_001228"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7429199.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            19548..20921
                     /locus_tag="IVO76_001229"
     CDS             19548..20921
                     /locus_tag="IVO76_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7429200.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            21278..23422
                     /locus_tag="IVO76_001230"
     CDS             21278..23422
                     /locus_tag="IVO76_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7429201.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         6845 a         4594 c         4211 g         7816 t
ORIGIN      
        1 tataaaaaaa aatcaaaata actgattatt tacgtttgaa taatgagaat ttttctatat
       61 attctctatt ttgataatag tattctcatt ttcaaaataa ataagggagg tgtgacaaaa
      121 ttcttgtcac acctcccttt tccgaataaa ctgtgttcca gaaatagctt cttcgaaaat
      181 aagctgaaat ttcacaaaaa tttgagaagc aattttcgta cattccttct tctttcttga
      241 aactgaccac ttctgtcaca ccctctcttc tatcactttc cttttttcac acgactcccg
      301 gtttctttga taaatctcga tactttcaat tttcttgaat ttttcatttg aggtacacat
      361 agatacagac gatttcttgc tctagtcatc gcaacataaa atagtcttcg ttcttcttct
      421 atcgtcgtca ctggttctgc ttttatttca ttaaagtcga ttactttctt ttctgtcgct
      481 ccgttataag gcaaacttcc ttcgttacaa ccaatcaaaa agacatttcg gtaactaaga
      541 cctttacttt tgtgaatgct ctggatacat agtgcatcaa gttttccttg tctgtaagca
      601 atcaagcggg tttttaattc tttagctgtc gcttgggttt cttgcgataa ggcttggagt
      661 cttctgaaag tagcatgctg ctttacttct tctcgtatcc attcttgatc actctggtcg
      721 atttccttag ctatacgtgg cagatgaaga tagctttgaa caagcgaata gatttcttga
      781 tgctggtctg ttttacgcaa cgcttcctca aactctcgga tttctgcaga tttcgatttt
      841 aaattggggc ttatcgacaa aatataatct gcaacggcta tattttgata tttaggagaa
      901 aagtattgat cgcattcact ttttttcagg tagcagtctg ccatcagctg ccggagcaat
      961 tgctggtagg cctgattttt cttttgcaga ttcagtgctg tttgccaatg aacaattttt
     1021 tgatagaaat gttggaaaga cttgatacca aagatacctt catatggact atctgcttcc
     1081 agtacaaaag gaatattcgt tttcagcaga gattggacaa gctgctgcgt ttgtgaagct
     1141 gatcggtata atatgattga ttccttatat ttcccatttt tttgaataaa tgtctgaatt
     1201 tgtttgatta gccactgact ttctgcttta gtatcagaaa acaaataggc ttccataaca
     1261 cctgcttctt tttgtgctgg aatcatcgcc tttggtatcc gatcattttt attatagctg
     1321 atcaatctat tgctccttgg acaatagctg cccgagaacg ataattagtt aataagaaaa
     1381 gagttttggt aggatattct ttttcaaagt tcttgatgta tgctggctct gatcctctga
     1441 atccatagat actctgatca tcatctccaa caacaatcaa cttgatcctg ttttcctttg
     1501 tcatccgttt cacgatttcc cattgaagtg gattagtatc ttgaaattca tcaatgaata
     1561 gataatcata tttttggccg atataatgct ggatctcctc attctccaaa gcgcttttca
     1621 gattttggag tacatcattg aactctagca attgctggct tcttttttgt tcttggagtt
     1681 ccgtgagcaa tccatacagt cgttctgata tcggctgtcg tgcattctta tcaatggaat
     1741 catatttttc tttaactaac tgaaccattt cttctactga aaggttcata ttctgccaat
     1801 agtctaaccg agaagcaatc gttttttctc catattgatc aaatatataa agattatatt
     1861 tacgtatcag ctgttgcaaa acagcattca ctttttgtgc agtatccttt gaaaagtctc
     1921 cttgtattcc ttgcattgct acttcaggaa attttcgaca aatttcattg tatatcctaa
     1981 aaaagatact atggaatgtt ccgatatgga cagcggtttt tctctggctg aaattatcta
     2041 ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt gatcattagt atgcgttctt
     2101 cgtctacttg tttttgcgtc atcagataaa cagcttttgc acagattgtt tgcgttttgc
     2161 cgctcccagc tcctgctatg attcgaagat gtttcttcat atcatatgtg atagctgctt
     2221 tttgttcttg attgaagcga ttataggaac gtttctccat cattttaaag aaagagctgt
     2281 ttccttcact ttcttttact tgtacttctt cctcaataag tccgggtgct tcgccataag
     2341 ccgcttcata ggtagcaatg atttcttctt gtagccgaaa gacttgttga agctttttat
     2401 acttgttctc ccagcgtttc aattcttttt gtaatcggag aatttctttt tcattcaact
     2461 gattgatttc cttcccatct ttcttctttg aatttgttta tttcaagttg gttatttaaa
     2521 gtttcgttgt ctattttttc tttatactaa cagacctttc taaaaatgaa aatgggaagc
     2581 gatatgattt tagttttctg aaaatttttc ctttttttca atcgtttatg ctaagataag
     2641 gaagacaaaa atattcataa aaggtgatga aaaaaatgca gacttccttt cacgaggggc
     2701 gtacaatagg acttttgctg acatttatcg gaggagccat ggacagttat acttatatcc
     2761 aatatggtgc ttttgcctct gctcaaactg gcaatatcat tctatcgatc atccaagcat
     2821 ttgacggaga atggctaagt gttggtaaaa aagtattgtc tacccttttc tttttcttag
     2881 ggattcttct aacaaagtat ctaattgatt attttagaaa aaaagaaaaa catttctggc
     2941 gcttgtttgt tctatattat gaagcagtga tttttttctt tgttagtttg acacccgtcc
     3001 atgagcatcc cgcattagta acgattttga ttgcttttac tgcatcgatc cagtgggtag
     3061 cttttgataa aatcaacggg cgtgcctaca ctaatttatt tacaactgga aatttaaaag
     3121 gggtagcaac gaacttatat gattattgta tttctaaaga aaaagcagat tttgatcgat
     3181 ttttccacta tctaagtgtc gttcttgcct ttatcgcagg agctattgtc cttgttttct
     3241 gtcatcatct gtttggcgcc cattcgattt tgatcgtttc cattttattt ttgatcatgg
     3301 ccctcactca gacatttcat ttatggcaat tttatcatac gaatcggttc actatttgat
     3361 ctcaatgaat caaaaaaact aagcaagatg ctgctcacag agaaatatct gcgagtgagc
     3421 aatttgctta gtttttatat tatttagttg taaagattcc ctttgctttc gggttatttg
     3481 tagtgtagcg gatcgactgg gcatctgcct cttctgtcat tttctttgcc cgcatcttga
     3541 tttcttgtac gatttgttct ccacatgccg gatcggctac gaaatcatat ttgtccccat
     3601 cgatcactat tttgggacat atatcaaact gttcatacca ctcttcgtat cgtcgattga
     3661 gttctttata gtaggaatag agttcaggat cttgttctag ctgttcatat tcccggcctc
     3721 tttttttgat tctcgctaac atagtttcaa aggatacacg aatataaacc agtagatctg
     3781 gtttcttttg tggcgaaatt tcttctaact ccttcagcat cgtttctaaa agaccttcat
     3841 actgctttac ttctacttca gaaacacgcc ctaaatctgc attcaaatga aacagcatac
     3901 tatcttcgta gatggagcgg tctagtacgt tgtcatcatg gctgaaagca tctttgattg
     3961 ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa agtatacttt tctggattag
     4021 aataaaatag cggcaatact tcattatctt ccacgttctc ataaaaaggt tttgtttcta
     4081 aatcttgggc aagcatttca gttagtgtgc ttttccctgc accgatcgtt cctgcaacaa
     4141 tgatcacaat tatcggcatc cttcctgtaa taaaatctgt tcttcatttt actacttcat
     4201 ttatagaaat aaaagccttg actttatctg ctccttaaaa gctacttctc taaaagtagc
     4261 caaaagtaac tcctattcat gaaaataaag gctgtgacaa aagtcttgtc gcagcccttt
     4321 tttcaaataa acagtattct cctgccaaca cctcggtcac aaggcaatat tcataaaaca
     4381 tgaaaagctg ttttttaaca ttattccttg taactcagag ctaaacggga gactcacaac
     4441 ctctttatgc tctcaacatt ttatctgttt ttattcttct attgtacttg atttgcgtac
     4501 aactaactgc cagtgcagat tatgagcggt atcattgtct tctactgcgc atttcatgac
     4561 acgtatcatc acactatttt catagatttt ttctattttt ccttttattg gtgattcaaa
     4621 taaatcagat ttaaatgcat atgtttcgcc gacttcaaac aattgttcat tttccataag
     4681 aatccctcca tcacctttta taaggtcagt aattttttaa cataaatcac tcgaaaaagc
     4741 acaaacttgt cataaaaagt acttttcgta tcaaaactgt cacttttttt ggtaaggttc
     4801 ttggaaaacg aagatttttt taggagatca tgacggtttt gatgtcaaaa ccacttttta
     4861 tgaaacaaaa gacgtttttt atttttttag cataaaattt gtggtggagg tacgtatgag
     4921 atgaaagata aagttgtgaa caaattatta gaagccttat tcagcggaga caaatcggaa
     4981 gaagatatgg tgtatgtcca agtaaaattt gccttagaag ttttattgaa taatcttgga
     5041 aaactagcgg tcgttctagt cttttctctg gtgacaggct catgggcgga aacaggaatc
     5101 acctttcttt cttatatctg catccgcaga tatgcttatg gattgcattc cgactctgaa
     5161 ttcgtttgtc tgctgtggac actactttat ttatggggcg ttcccttggt gatgaaacat
     5221 ctgcaattga ctatctcctt cccaatgatg gtctttttgt taatcagctg ctttttgtta
     5281 ctgcttcgct atggatcaag aggtacagca atcaatccca ttgaacccga gaaacgtccc
     5341 ccattattaa agaaggcgat cagcatgttt ctaatttttt ctctcattac tttatttttc
     5401 tcagcttctt acttttctac ctatttgcta cttgggattg ttttagaaat agctacgttg
     5461 cttcctatta caaactactt gatgaatttt ggaggaatct ttcatgaaaa aaacaatcat
     5521 taaacaatta actaacttat ctgacttttt tgtatctggc tgctgctttg gtatgagttc
     5581 tgaattcaaa atccctgaaa aattaaaaaa ataatacgct aattgtgcta taatttggta
     5641 aaaatcaatg ggagctgtac tttatgtttt tatggtccat agcgttgcaa tatttgattt
     5701 acacaatttg tatcttattg attgaccatc acatccgata caaatatctg tttgcgggag
     5761 tcattctgat ttcttgtatt ttgattggat gggtctattc gattttaggg atttttactg
     5821 ctctaattgc tataggaatg ctgatgctta cttttttctt cttttcgact tcgcgtcaac
     5881 aattggttta cgcgcttcct tttgggttat tgacgtcgct tttaggagat cacttaacaa
     5941 gcatcggcga tttttttgtt ttgaaatctt cggttattga accaggagat tcattgcagt
     6001 attttcacat tgtcttatcc gcttttttat ctgctttatt agctttttgt ttaaaaaaat
     6061 cgttagagac ttggtttcac actgtggacc gccgtatgat cggtacgatc gggacattga
     6121 tattatttac atactatatc attatttttt atacccgttt ttctggtgaa acacctgatg
     6181 tcttagcttt gaatacgagt tttttcattc tatatctgtg tgttggcctg atcatcttgg
     6241 tttattattg gaaaatctct aataagaaat tagctgatca gttattagag caacaagtca
     6301 ggatgcaaca ggattatatc aaagatctag aaaaaaacta tcaagacttg agagaattca
     6361 aacatgatta ccaaaatctg cttttttctc ttcacagcta tatttcagaa ggcgacttag
     6421 aaggattacg gtcttattat gatcaaacga tattacctac acgccagatg atgaaccttt
     6481 ttccggccaa tctaagtatg ttggataata tgaaagttcc tgagatccgt agtttactgt
     6541 cattaaaact catgatggca caggaaaaag ggttgatcgt ccagcttcat tttcctgagc
     6601 caatcgaact ggatacgaaa catacggtaa accttgtacg tatgcttggt attatcttgg
     6661 acaatgcgat tgaaggagct agtgctgcaa agaagaaaaa aatcgaattg accatactca
     6721 agaagaatca ttccgtgatg attcgtgtga tcaatacaac aacaaataca ttaccgttaa
     6781 accagttgaa acaaaaaggg ttctcaacaa aaacaaatcc aaaaaacgaa ggccttggct
     6841 tgtttatctt ggatgaattg acaaagacca atccttatct tttccttgaa acatcaattg
     6901 aaaaccagcg tttttctcaa acgatttata tacaaataaa ataaaggagt aatgatatga
     6961 ttcccattta tatttgtgaa gacgatccaa aacaattaga agttttatca acggtgatca
     7021 aaaatagaat catgattgaa aacttagaca gttatgtcca tatttctacc tctgatccgg
     7081 aagaacttct caatgcagcc aagatcagaa cttcttcttt tggtatttac tttttagata
     7141 tcgaactaga agacagtgaa atggatggtg tagcgatcgg taaattgatc cgtgaattag
     7201 atcctttagg aaaaataatc tatgtgactt cccatacgga aatgtcgatg aaaatcttaa
     7261 aaagcaacat cgaaccgact gattatatcg tcaaagaaga tctttatgat ttaaaagaaa
     7321 atgtcgagca tatcctgagc aaaattttcg aagattttca aatgtcccaa atccaaaaag
     7381 atatcttcaa aatagagttt aatgatgaga tcaaattttt accgatcaaa gacatccaat
     7441 atttttctac ttcgctagga gcgccacata aactggaagt ccatttgact catgcccaat
     7501 tacaattcta tgagaagatc aaagaaatcg agaaaatgca tcggaatttt atccgttgtc
     7561 ataaatccta tgttatcaat acacaaaatg tgcggtcgat taataaaaaa accagagaag
     7621 tcacgttggc aaacggcgag accatcccag cctctatccg tggattaaag aaattgattt
     7681 cataaaaaga accgaatagt ttggaccgac taaatgacca gccgatccaa acccttcggt
     7741 tcttttttgc ctatcagtgc aaaagcattt cctgtgcaat ttcacgtcca tccattgtat
     7801 ctggataaaa tgttggccaa ttctccattt ctttcagcaa tacttcttga ctttcatttc
     7861 ctgcatataa atgaaaatgt tcggagtcag ccggtgcaat cgtgtgatca ctgaattgaa
     7921 tatatctaaa agcgccactt tccttatctt ctgcttcgaa tagatacctt acgccacggt
     7981 ttcctgcctc gtaggtcaag atttctttcc ctacataacg ataatttgaa cgtttcgttg
     8041 ttccatcctt aaacgtaaat gccatctctt tctcactgat cttgattgaa gcaatatctg
     8101 tttgatagcc cttcgtataa tattctttat attcttgtgc tgtcttatcc tgattcaatt
     8161 tttctttata ttcaaaaaca ggatcaaaag tcccatcttc tacatacgta tctactgttt
     8221 gccaagttcc tgcccaatct gaaagagagc gatcttgtac atcctcgtct tcaaaataac
     8281 cgttgtagac gctcttttct tttttggggg tcaaagtatc ttctagcggg ttttctgtct
     8341 ctgtcgtttt tttcaacgct tcgaggtttg cttccatgat agaaagatag ttttctccat
     8401 tttctatttg ttcattcgtc aatccttcca aaggattcaa tacttctaaa gatacacctg
     8461 cttcgtttgc taacgttctg gcaatactat ctttggcgtt ttcttcaaaa taaatatagt
     8521 tgattccatg ttctttaaca aagtgtttca gttcagcaat tctcgccgca gacggttctt
     8581 cactagaaga aagaccagca atcgatactt gattcaatcc atattctaac gctagatagt
     8641 tgaatgcagc atgttgcgtg acaaaagatc tttgttttgc ttgcttaaag gtttcttgat
     8701 aaagctgatg gagtgcttga agtttctcgg tataggctgc tgcatttttt gtccatactt
     8761 cctgtttttc tgggtaagct tcaatcagct gatcacgtat gtttgctacc tgcttgattg
     8821 ctaaacttgg agctagccat acatgtgggt ctagctcatg tgtatggcct tcttccccgt
     8881 gttcatggtc atgatcatgt ccttcttcac ttcccggaag taaaagcata ccttctgtcg
     8941 cttttatcgt atggacattg ccttcttgta atgttttttg gattgcgggg acccatgttt
     9001 ccatgttttc atcattataa acaaagatat ctgtatcttg gatctttgcc atgtctttag
     9061 cagaaggttc ataatcgtgg gaatcagtcc ctgctggaat caataactct acttctcctt
     9121 catccccgac gattgctttt gtaaaatcat acatgggata aaaggtcgtc atcacgctca
     9181 atttttcttt actgtcctcg gctttcccac ttgccccgca agcagttaac atacctgcta
     9241 aaaccagcat ggccaatata cctatcaatc gtttcattta tgacactcct cttctcatct
     9301 tctttcattt gaatgttccg attcgctttt cgcaaaccgt aatagttacg ttttagcaac
     9361 aggatttaat ttatcatatg gttaaaagaa ctgtcaagcg ttttcttatt ttaattgatg
     9421 agaaagttta tatgaatgat tgacgatatt tttgcaatca acctctatca ttttcctttt
     9481 atacccgctt ttgtctataa tgaagttata gataaaggag gaaaaagagt gaaaaaagaa
     9541 acagatacaa cgaatcgttt gatccgcttt ttaggaggaa aaacctctta ctatgtttta
     9601 ggaatagtta tcttacttgc gctgatcatt tattttttcc atcagatctc gtttatattc
     9661 aagcctgtcg gaatcattat cgctactatc ttgcccccat tattgtttgc actgatcttg
     9721 tattatcttt tggaaccagt catcaaacgt ttgagcaagc acctttcgag gaacttgtca
     9781 gtcattatcg tttatgtgat tattttattt ttattggggc ttggaagcat atggcttatt
     9841 ccttttcttg aagaacaggc aaagacattg atcagtagtt tgccagattt gtttcaagat
     9901 tttcaagaga gtctccgaca atttttggag aaaacacctt ttgccgattc atttaatcaa
     9961 tttttcgctt ctattgacga cgtgacgtct gatatagctg gatttattgg aaattattgg
    10021 gaaagtggcg cacaacggat cagcaacatt ttttcaactg ttacagctat ttttattact
    10081 ttatttactg gtccaattgt cgcttttttc cttttgagag atcctcaaaa attttatcag
    10141 tcagttttag cgattgtccc accagcattt cgtacagatt ttaaaaattt gacaaagatt
    10201 gctaatcaac aattgggttc atttctaaag gggcaaatca ttgcttcatt tatccttgga
    10261 gcagtctact gggtttgttt cttactgatc ggattagaat ttgctagtgt tttagctatt
    10321 tctgctgggt tgttatgtat cattccatat attgggccgt ttatcgcctt ctttccaggt
    10381 ttgatcattg cattccaaga ctctactttt atggctgtca aatttgtcat tgtctggttt
    10441 gccgtacaac ttctccacgg tgacttggtg attcctcgag taatgggtga tcgtttacag
    10501 atccatccga ttacgatctt aatcgtttta cttgtcatgg gtgatctaat gggaattgtg
    10561 ggcgttattt ttggtattcc tatctatacg ttagtcaaat tactcgttac ttttattttc
    10621 cgtaaattca aacaacgcta caataaattt tatggcgata aaggagagta tgaaaactct
    10681 tcattttctg aagaagatta ttttaaataa aacacaaaac gtcgatctta cgagaatagc
    10741 tcgtaggaac gacgttttgt gtttttgttt taatttttca gcaaagcaat tgcctggtat
    10801 ggttttagct ccagactttg atgaagaaca gtatcggaat aattatcgat aagaatattg
    10861 gcttctgtga atccttctgg gatggcgatc cttgtatctt tatcatagaa atttgttaat
    10921 acaagcagct gctgtccttc aaattggcga agaaagccat aaacttccgg atgatttgct
    10981 tcataagcat gataatctcc ttgtgcaacg attggatatg tttttctcag ccgaatcaat
    11041 ttcttgtaat aggacaaaat agaatcttca gaagatagtt ctttttctac attaatctct
    11101 tctagcttgc ctccaacttt taaccaaggt ttccctgtag taaatcctgc ttgttctgtg
    11161 tctgaccatt gcatgggtgt tcgagagtta tcgcgagatt ttgctttgat ccgacgaaac
    11221 gcttcttttt ctgtgaatcc ttgttcaagg agtaacttat aagcattcaa actttctacg
    11281 tcaatatagt cggaaatcga tgaatagtct gggtcgatca tgccgatttc ttctcccata
    11341 taaatatagg gcgtcccacg gttcaaatgg atcatggttg ctagcatctc tgctccttgt
    11401 tttcgatact gatcgatagc tacaaatcga ttgagcgcac gtggctgatc atgattatta
    11461 aaaaatagag cattccagcc gttaccctca ctcatctttt ctccccatgt atgaaatagt
    11521 cgctttaatt ctgcaaaatc aaatggagga gtagaccatt tattcccatt ttcataatcg
    11581 actttcaaat gatggaaatt aaagaccatt gataattctt ctctactagg ttctgtatac
    11641 aagatacaat tttcgatcgt cgtagagctc atttcaccga ctgtgatgat agtggaatcc
    11701 gtcccaaacg ttgcttggtt caattctttt agatacgtat gggtgattgg tttgtccgta
    11761 tattcaggtt tgccatcatt ttctttatta tttttcaatt gttcgtcttt accgataaca
    11821 ttgataacat cgaaacggaa tccttttacc cctttttcca tccaaaaacg aacaactttg
    11881 aatagttcct ctcttactgc ttggttccgc cagttcaagt cagcttgtgt ccgatcatat
    11941 aagtgcaaat aatatttccc cgtatctcca aatggcgacc aagcacttcc accaaatttt
    12001 gatacccaat ccgttggctc ttctcttaaa atgaagaagt cctgataatg ctggtcccct
    12061 gctagtgctt ttttgaacca ttcatgttct gtcgaaacgt gattcagcac catatcgagc
    12121 atgatttcta tccggtattt ctttgcttcg gctattagct gttcgaattc ctccatcgta
    12181 ccaaagatag gatcgatggc tgtgtaatca gagatatcat aaccgttgtc tttttgaggg
    12241 cttggataaa tagggtttag ccaaatcatg tcgatgccta gatcattcag atacgggagt
    12301 ttttctttga ttccttttaa atctccaatt ccatcaccat tactgtcttt gtaagatttg
    12361 ggatagattt ggtaaatgac tttgtcttta aatgtcatgg atatttacct ccattcaact
    12421 gatttattac ctaccacaac ttatatatac aagttatcat ttattattat aactgaaccg
    12481 aaagcgtttg taaaatttat ctttatacat tctaagaggt ctgttgccca ataaaaaaca
    12541 agagatcaga catgtatttg tctatcctct tgtttaaaat agcaaaatat tcttgtttct
    12601 tctataaatg gtgttcaaaa gcttttatct acggcaatca gtcttctcaa atgtaaaaca
    12661 tgaggagcta attttatttg agtattcttt ttgctcgatg caggacggct tttttacaac
    12721 ctctgattca tggtgttcaa ttagcagctg accctcgtta ttagctcaaa acgctgattt
    12781 cacaacctct gtttttattc attcgatttc aacgcatcaa tcatatcgat atgctttaat
    12841 ttgaaatggg taacaatcat cacaataacc gtaaatacga tagttaatcc tgctgataag
    12901 acataagccc accatgtgat cactaatgga aagatgacat tctccataga cgcttgttgc
    12961 aagatgaagt cagtcaatat atatcctaca ccaaatcctc cgatgattcc taaaagagta
    13021 aagatgatat tttctcgaac aatgtacatc gtgacttctt tatcaaaaaa tccaaggact
    13081 tttattgtag ataattcgcg tacacgctcg gaaatattga tattagttag attatacaag
    13141 acgataaaag ccagcagtcc tgataatacg acaaagatta agacaaccga gtctaagttc
    13201 gccattgatt cttcttgcgt ttcgatttgt ttggacatga aagacgtatt tgtcacagcg
    13261 tctgtggcaa gcagtttctc ggaaagcatt tcttcttctt ttgttgtcat cttatctgta
    13321 cgcagcaaaa atgcattatc catcatcgat tgatcagtaa ctgaagcata atattcaggg
    13381 gacatataaa taaagctacc tagatagttt ttcacgattt cttttacttc gactttccat
    13441 tctttttgat cttcatcata aagcgaaata tgatctcctg tagataagtc aaatagttct
    13501 gctacttttt gcgtaatagc gattcctgtt ttaggcaagg agcgtgggtt ctcttcaaga
    13561 tcagttaaat ggataaaacg atccaaatca gctgttttac ttggtacgat cactgtcaga
    13621 ctttgcgaag catgtccttt ttttctcaat tctacggttt gagtatgaat agctaggatc
    13681 tcatttactt tttcttgctt ctgcagcata tccgctgctt tttgacgact atcttccgtg
    13741 gatgaatcca atgtaacgat tgcttgatag tcaatgatcg gtccaaattg tttggctgaa
    13801 acagaactta gagaatcttt caatcccacg cctgcaacca tcagcccggc acatccagca
    13861 attccgataa tcgccataag cattcttgac ttatatcgga agagatttcg gtaactgatt
    13921 ttttgattaa agctaaggcg tgaccacagc ggagtgatgt attcaagaaa aatgcgtttc
    13981 cctggcttcg gtgctttcgg ttgaagcaat gcagcaggcc gttctcttaa atctttgaat
    14041 aaaacgatca ttgcagcgcc aaatgcggca atgaagaatg cggcagctgc ttgtaaaatc
    14101 ggcggccagt cataaaaaat aagcgcactc ccaatatcat accgctcgtt agacagctca
    14161 aaaatgatcc ttggcaaaag attcgttcct aatatcgttc ctaaaacaat acctatccct
    14221 gaggctagac ttgcatagat tgcatatttt tttgcgattt caactttcgt ataacctaac
    14281 gcctttaatg tcccgatttc cctgcggttt tcttctacca tacgtgtcat tgtcgtaaag
    14341 gtgatcaatg cggcaataaa gaaaaagaaa acaggaaaaa cattggcaat cgcagcgata
    14401 cgatcagaca gatcaccaaa ttcttgaaaa ccgacatttt ctgaacgttc attgtatagg
    14461 tatgatggtt tagttaaatt ttcaattttt tctgcatttc gattcaattc ttcctctgca
    14521 gaggccagtt cacgttcttt ctcaactaat tgcctttctt gttcagcaag ttgagcttct
    14581 tgttgagata atgtttgaac agcgattgtt cgttgcgctt caggaagctg ctggatagtt
    14641 gctgactgtt gcgctaactg ttttttagca tcttctaacc gtgttcttgc ttgcgttgtt
    14701 tgtgctttgc catccgctat tttttgctgt gccggttcta attcttcgtt ggccgatttt
    14761 ttcagctctt ctaagcgttc ttcgggtcga tcttgtaaat acttttctgc cttttcttga
    14821 ttttgctcca ttttatcttt gtaagctttg ctgtacgtat tcaaattctt tacgtttgta
    14881 aaatcaagat ataagacaga ataaatatct gttttaaaaa cggattcagg taaatagaca
    14941 aaataatcga cgctaccatt tccgacgttc gcgtatcctc tctccaaatt gttgatgaac
    15001 aatggagaac ggacaaagcc cgtgatggta tactcttttt ctttcaattc atctgaatca
    15061 atctgataaa tatcaccgat tttataccct tcatcttccg ctttttcatc caatacaagc
    15121 tggtttggtt tttccgggag ttttccatca gttaattgta gcttgttctg cttgttattt
    15181 ttgttataag aaaatatttg aatcacttcg tttttgccag tattggaata atagatctgt
    15241 tttcctgttt ctacttttgc atctgggatc ttttctgctt gagcaatatc tttttgtgtc
    15301 aagccaccag tagaaacgat ttgcaaatca gaaagtcctg ctgtaccaac atagtgatcc
    15361 atcgtttttt gcatgacagg tccagcagtt ttgacaccta caaataaaag tgttcctagc
    15421 atgacaataa ggatgattgc aatgaatcgt cctttagatt gccgaatttc gcgaaaacct
    15481 gctttaagat atgttcttgt tttcataaaa tccccctacc atacaatctc atcaatggat
    15541 gaaggatgat catttaattc aacagacctt actttagcat cattaatgtg aatgactcta
    15601 tcagcgatgg gcgctaaagc agaattatgt gtaatgatca aaacggtatt gccgtgttgt
    15661 ctgctagcgt tttgcaataa tttcaatact tgcttgcccg tttcaaaatc cagcgcgcct
    15721 gtcggttcat cacataaaag aagtttggga tttttagcta gtgcacgagc aattgaaacc
    15781 ctctgctgtt cgcctcctga taattgggac ggaaagttat tcaatcgatg agctaggccc
    15841 acttgcctca atacctctac aggatcaagc gcatcaggag aaacttccgt tgccaattcc
    15901 acattttctt ttgctgttag attaggaacg agattgtaga attggaaaac gaaccctaca
    15961 tctgttcgtc ggtaagcagt aagctgttta tctgaaaatt gcgcaatatc tgtgtcatca
    16021 ataatgatct gaccttcatc aggtgtatcc atcccaccta aaatattaag aatcgttgac
    16081 tttcctgctc cacttggacc aagtataatc actaattcgc ctttttcaat tgaaaaagaa
    16141 atatcgtcat ttgctgtaat ggtcgtttct cccattttgt atcgtttgta ttcatttttt
    16201 acttcgacat acttcactct gattcctcca ttttcacgat atttctcgct atatctatgc
    16261 atcgaatgcg ttttctcaca cttatttatt ttaccactga atttctctag actaaagaaa
    16321 ggaatccttt aatttagttg acaaataaac gatttatgat ttacttacat aaaaaacatt
    16381 gttatttgaa agaaggaaca tcatgaatga tttaaaggaa aatatgattt ttgatttatc
    16441 aaaaactgct tttgtcgcga tcgacatgca aaaagggatc gtaaataacg gtacactctc
    16501 tccacacact gcacaatcta ttctgacaaa caatgatttg ttagcaaaaa cattgaaacg
    16561 taccgctgcc ttgaaagttt tagtgaatgt agacatcact actttctctt atctaagtca
    16621 tcagacagat gctggaggaa tatctgcttt gtctgctccg gcagaattca ctgacttgtt
    16681 gttgaaggat tccttgaaag atacaaacaa tatgttgact atcacaaaat acaatccaag
    16741 tgcctttttt ggtacttctc ttgatcttca acttcgccgg cgtggaatcg aaactatcgt
    16801 tttaagcggt gtagcgacaa caaacggtgt atatgctact gcattagatg ccttccaaca
    16861 tgggtatcat gttgtacttg ctgaagatgc ttgcagtgat cgtgacaaag aatcccatca
    16921 attgttcata aaaaagatat ttccgaaaac tgcacgagta cgctcaacga aacaaataat
    16981 cgaagctatc caacaaagca aataagtact ggcacacgtg attttcattt acttcttttt
    17041 gatttcatgg gaaaataacc acaggattta gataggagta tgaacatggt gaaaaaaaag
    17101 atcacaatca aagaagtcgc taaactttcc ggcgtttcaa tcacaactgt ctccttgata
    17161 ttaaatggaa atacggagaa attcagtccg aaaactgttc agaaagtttt agctgccaaa
    17221 gaagaattag gctatcagcc tgactatttt gctagacaaa tgattaccaa ggaaacgaaa
    17281 acaattggtg tactcgttcc tgatatcacg aatccattct tcagtacact gatgcgagga
    17341 attgaggata tcttgtataa gcagaatttc gtcacgatat tatgtaatgc tgattctgat
    17401 catcaaaaag aaatcgaata tctagccgag ctcacacgtc gaggtgtgga tggattcatt
    17461 atcgcgactt ctgctgtctc tacagatgca atcaacgaaa atttgaaaaa acaaggccgg
    17521 cccttcattg ttttggatca gaaaaaatct gaaggattta gtgatgccgt acgaacagac
    17581 gattttcgcg gtggttacct agcgggcatg cacttgctta gtctaggtca tcaaacgatc
    17641 gcccttgtct atcctgaaaa tccgccggaa aatgtccacg ctcggattga ggggtttaaa
    17701 tctgctcttg atgtttacca aatctctcac gaccaactga tcctcttacc tacacagttc
    17761 tcaaaacaag gtggctacca aatcacagca gaacttcttg attctgctgc aactggtgtg
    17821 tttgctttaa atgatgaatt agcttttgga ttatatcgcg gattagagga atctggaaaa
    17881 agcattcctg aagactacag cattattggc tatgataata tagatatgtg cgagtatatc
    17941 aagccgaaac tgacaacgat cgcacaaccg atcttcgaat tgggacaaac atctgccaaa
    18001 ttattgttag atcggatcca attcccagaa aaagaatggg aagaaaaaag attacctgta
    18061 cgattagaaa aaagattttc aactgcaccc ttgaaatagc tttcaaggtg tgctatattt
    18121 ttatcgaact attaaaacgt tttactaaaa cgttttaaaa aataattttt cccatcaaaa
    18181 taaatgagat agttggaggt ttgtaaaatg aacacggtta cagtaattgg aagtattaat
    18241 atggacacta cccttcgttt aaccaacatg cccaagccag gcgaaacaat gcatgcgcat
    18301 gagatttttc acgctggcgg aggtaaaggt gccaatcaag cagtggcagc caaacgttca
    18361 ggcgcacgga catcttttat tggaggcgtt ggggctgaca gcgaaggaca acagttatta
    18421 gatctactga caaaagaaaa catcgataca tctgggatag ctgaaatcca aggcgccaca
    18481 accggacaag caatgattat ggtagacgca gcaggagaaa atagtatctt gatccatgca
    18541 ggtgcaaata atgctttcca tgaacaagaa gtcctaaaaa acaagcaact gattacaaac
    18601 agtgacttta ttattgctca gtttgaaagt agcttagatg cgacgatcct agctttttct
    18661 attgcaaaag acgcaggaaa aacaacgatc ctgaatcctg caccagctcg tgaaacgatc
    18721 cctactgaac tacttgaaaa aacagatatc attataccaa atgaaacaga aacagaaatc
    18781 attacaggaa tcagagtgac agatcataac agtttggtcg cagcagccga aaaactccat
    18841 gaattaggta ttggaacagt aatcattaca ctaggcagtg ccggcgcttt ctatcataca
    18901 gaaaaagaac acggtatcgt ccctgctttt aaagtggatg ccgttgacac aacagccgca
    18961 ggtgatacat ttattggcgc attaagcagc acacttcagc cagaccttag taacttgaaa
    19021 gaagcgatcc tttatggaaa tctagcctct tctgttgctg tgcagtctta tggtgcgcaa
    19081 ccttctatcc cttaccgcga agctttagaa caatcagcag tctaaaatta acaatgagag
    19141 gaagaaacaa acatgaaaaa aggaaaagta atcaattcag atatttcacg agtaatcgca
    19201 caaatggggc actttgataa attaagtatc ggggatgcag gcatgcctgt cccctttggt
    19261 actgaaaaaa tagatttggc tgtctcaaac ggtcttccaa gctttttatc tgttttagaa
    19321 aacgttttag aagaacttga ggtacaatcg attgaattgg cggaagaaat aaagacaatg
    19381 aacccggaaa tattggaaaa aatcaaatta ttgttaccag atacaccgat tagctttatc
    19441 ccacatgaag aaatgaaaaa agagttgaat aactgtcatg catttattcg tacaggcgaa
    19501 atgacacctt acgccaatat tatattagtg agtggagtgg tcttctaatg gaaacgacaa
    19561 tgaatcctga acagatggta ctgtccgagc agattcatat gctgattgaa cagttgggag
    19621 gaaaagacaa tattgattct gtccttccat gtgcgactcg gcttcgtatc aaattgaaaa
    19681 aacagccagt agccaatgca gaaaaattaa aacagcaaat cccttccatt aagtatttca
    19741 ttttactcga tagatatgca gaaataatca tggatcaggg attggctcct gcttacaagg
    19801 aattaacgcg tcaaatcgat cattcagctc ctaaagaaaa gctaaatccg atcgaaaaac
    19861 tatttggttt gatatcggaa atctttacgc cgatcattcc gctgtttgcc ggttctggta
    19921 tattaaaagg tctggttgtt ttagcgacta cgattggctg gcttagtgaa acaagcggaa
    19981 cttatcatat tttgtctgct gcgtcgaatg ccgtctttta ttttctgcca atctttcttg
    20041 ctttttctgc cgccaaaacg tttaaagtga atggatatat tgcggctgtc attgctgcag
    20101 cactgattga accgaatctg acctctttac tttcagacag tggcaaagca gctactgatt
    20161 ttctggggat tcctgtagta ctgatgcagt atacgtctac tgtcatgccg gcgattttag
    20221 gtattttctt ttattctttt gttgaaaaat tcctcaaaaa atatattcgc gaaaatatgc
    20281 aattagtatt tgtaccttta ctttcattag ttatcacggt tcctttgacg ttaatggtat
    20341 tcgggcctgc gggtgtttac cttggcgaag gtttagctgc cgcaattact tggatgatgg
    20401 atattaacgg accgatcagc ggcgcaatca tcgctggtgg ttggaatatt ttggtaatat
    20461 ttggtatcca atgggctgta aaccctgtca tgatcagtaa tatctctgct tatgggttcg
    20521 accgaatcgt tccgttgaca ggagcagcca attttggaat ggctggtgca gctttaggtg
    20581 tatttctacg aagtaaacgt tcaaaaactc gttcgatctc cggttctgct tttgcttcga
    20641 ttttacttgc gggagtgacc gaaccaactg tttacggaat tgcgatccca ttgaaaaaac
    20701 catttgttgc tgcttgcatt ggtgcagctg ctggtggagc tgtaatggga tttgcccaag
    20761 tgaaagcgat cgcttttgta tttggtagtc tgaccacatt gcctgcattc atttccggta
    20821 cttttttctg gtacttagca ggactggctg ttagtctagt cgtagcaatg attactactc
    20881 tcgttagcgg gtttgatgaa gacctaatgt cttatgaata gttaatgaaa aagactgaag
    20941 tatgagatgt ttatcacata tttcagtctt tttcttttta ggatcattat ttttataaat
    21001 ttttgtgata ctttccaaac tttggtataa tttacatatg cataaccatt tttatcccca
    21061 cttgtttatc cccaaacaaa ggtggtctat gcaataaaat gaacgtcaat gaaaaacgaa
    21121 tgaatgctcg ttgcttattt acaaattgtt ttatttagaa tacacagaca gatttatgaa
    21181 aagaaaaagc ttttcgacac gattatctgt tccaaaaaat gtctaatgaa tgctgaataa
    21241 gacagaaaat caatgaaagg aaaaggacta gtttattatg aaaaataaaa aaacgatacg
    21301 tttgctagct gctacttggc tgattgctcc gtctgttttg tcgaccaata ccgtatttgc
    21361 cgtagataat caaacagctg atcaaacagt cacaacagat acgactacat ccagctcagc
    21421 tactagtgaa acaacggatt cttttacaga agacccaact ggaacaacta actcaacaac
    21481 aggatcgacc gagccaactg aaattgatcc ggctgatcca tcagaaaatg aaaagccagc
    21541 cgagacatat tcccttacaa ttgctccttc tttgattggt aagatccaac tgactgcttc
    21601 gacagcggat tcccctgaac agcaaccttt ggatattacg gaaagtggaa cggtagaagg
    21661 tttattagcg gaaacgaagg taacttacac tattacgcca gctgaaggtg aacaaattgc
    21721 ttcagtcgct gttaatggtg ccaaagaagt aaattatgat gcaaagacct ttacagtagg
    21781 tacgaacaat ttatctatta ctgcagaatt tgaacctgta ccggatactt caacagagaa
    21841 accaactgat cctagtgaag aagtccctag tacaccagat ccggaaacgc caaacaaacc
    21901 agatgtgggt aataacaaca ataataatgg ttcaaataat tcaaacaata ataacaataa
    21961 ttcgaacagc gggaattctt cgaatcaaac aaacccatct ggatcgaata acaatggaaa
    22021 tactcagaga gaaccaaacc atccaaacaa tggttcatca aatggcggaa cgaacaatgc
    22081 ttcatctcgt ccaaatacag caaatcaaag cggtatagag aatccatctt ctgcttcttc
    22141 tgattttgtt gtaaaatcac caattgatgc tgtattgcct acaaatacaa cgaatgtcca
    22201 acaagcaatc gttcgagaag cattcaagca tcttggaaaa ccatatgtat ggggagcaaa
    22261 aggtccaaat acttttgact gttctggttt gacttattac gtatatatga aagcaaccgg
    22321 tcattatatc ggtggctgga caggcgaaca gcaatatgca ggtacacaga ttccagtaag
    22381 tcaggcccaa cctggtgatc tcgtcttctg gggaccttct tctggtgtta cccaccacgt
    22441 aggtatctat attgggaacg gacaatttat ccatgcgcct caaccaggag ataaagttcg
    22501 agtaacttct atttcagatt tcacgcctga ttttgctgtc cgagtaaact tagcaggttt
    22561 acctgctgct tcaggttcgc tgacaggcgg ctcatctatt ttagatggat tagatggctc
    22621 gttccatttc agtcaaaacc aaacaactga tcagttcttg aaaaagattg cagatgatgc
    22681 acaggaaatc gggcaaaaag aaggaatcta cgcatcagtc atgatggctc aagctatcct
    22741 tgaaagtgga tctggaaaca gcttattatc aagcgaacca aatcataacc tttttggtat
    22801 caaaggatca tataaaggaa gcagcgtgac cttcaataca cttgaacaag atagttccgg
    22861 acaaagctac caaatccgcg cacaattccg taaatatcct tcttacaagg aaagtttgga
    22921 agactatgcc gacttgatca aaaatggact gactggcaat cctgatttct acaaaccaac
    22981 ttggaagtcg gaaacaaaag actacaaaga agcaaccaaa tatttggaag gacgttatgc
    23041 tacagatcgc caatattcac aaaaattgaa cgcgatcatc gaagcgtacg acctgacaaa
    23101 atatgatgaa ccgaaaaaag aagcagcaac aactgaagaa gcaaacgctg aaatcctcaa
    23161 tatgtctaag cgattcgatg tgccaatcaa atggacaaca gatggcgtgc caatcacagg
    23221 attagaattt tcaacgataa aacgcgtcaa caacgcttct tccatcttcc gtgtcgcttc
    23281 aatctgggat ttgtggaaca attttacggc aagacagata ccagaaacaa ctactcgtac
    23341 agttaaagct gcaactgttc ctgtactaac cttgttaagt ttagacaaat cattctggct
    23401 tgatctgcct ttaaagaaat agaataaatt aaaaagggga tgtgacactg tcacactccc
    23461 ttttta
//
LOCUS       DACCQI010000041        23371 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCQI010000041 DACCQI010000000
VERSION     DACCQI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.041"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1226
                     /locus_tag="IVO76_001231"
     CDS             <1..1226
                     /locus_tag="IVO76_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7429202.1"
                     /translation="NNVRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSD
                     FREGLAAVLSIRVPENLLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQM
                     LVRKAIKAREAREAARKAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGD
                     SAGGSAKQGRDRKFQAILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSI
                     EDCNYDKVIIMTDADTDGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGK
                     KQVIEYAWTDDELAAMIKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRID
                     DAAQAERRVTTLMGDKVEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDE
                     ERADKNENNQLFEVE"
     gene            1238..3688
                     /gene="parC"
                     /locus_tag="IVO76_001232"
     CDS             1238..3688
                     /gene="parC"
                     /locus_tag="IVO76_001232"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7429203.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTNVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            3965..6196
                     /gene="pflB"
                     /locus_tag="IVO76_001233"
     CDS             3965..6196
                     /gene="pflB"
                     /locus_tag="IVO76_001233"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7429204.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            6440..7201
                     /gene="pflA"
                     /locus_tag="IVO76_001234"
     CDS             6440..7201
                     /gene="pflA"
                     /locus_tag="IVO76_001234"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7429205.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            7286..8212
                     /locus_tag="IVO76_001235"
     CDS             7286..8212
                     /locus_tag="IVO76_001235"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7429206.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            8394..9287
                     /locus_tag="IVO76_001236"
     CDS             8394..9287
                     /locus_tag="IVO76_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7429207.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            9421..9546
                     /locus_tag="IVO76_001237"
     CDS             9421..9546
                     /locus_tag="IVO76_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7429208.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(9680..10312)
                     /locus_tag="IVO76_001238"
     CDS             complement(9680..10312)
                     /locus_tag="IVO76_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429209.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(10460..11002)
                     /locus_tag="IVO76_001239"
     CDS             complement(10460..11002)
                     /locus_tag="IVO76_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7429210.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            11137..11526
                     /locus_tag="IVO76_001240"
     CDS             11137..11526
                     /locus_tag="IVO76_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429211.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            11530..12294
                     /locus_tag="IVO76_001241"
     CDS             11530..12294
                     /locus_tag="IVO76_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7429212.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            12423..13370
                     /locus_tag="IVO76_001242"
     CDS             12423..13370
                     /locus_tag="IVO76_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7429213.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            13500..14240
                     /locus_tag="IVO76_001243"
     CDS             13500..14240
                     /locus_tag="IVO76_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429214.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLRGLIDDQGNSLGENSYFK"
     gene            complement(14531..15199)
                     /locus_tag="IVO76_001244"
     CDS             complement(14531..15199)
                     /locus_tag="IVO76_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7429215.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            15333..15620
                     /locus_tag="IVO76_001245"
     CDS             15333..15620
                     /locus_tag="IVO76_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429216.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            16036..17574
                     /locus_tag="IVO76_001246"
     CDS             16036..17574
                     /locus_tag="IVO76_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7429217.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            17571..18536
                     /locus_tag="IVO76_001247"
     CDS             17571..18536
                     /locus_tag="IVO76_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429218.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            18523..19845
                     /locus_tag="IVO76_001248"
     CDS             18523..19845
                     /locus_tag="IVO76_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7429219.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      19908..20012
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            20086..20829
                     /locus_tag="IVO76_001249"
     CDS             20086..20829
                     /locus_tag="IVO76_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429220.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            20844..21836
                     /locus_tag="IVO76_001250"
     CDS             20844..21836
                     /locus_tag="IVO76_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7429221.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            21848..22585
                     /locus_tag="IVO76_001251"
     CDS             21848..22585
                     /locus_tag="IVO76_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429222.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            22575..23297
                     /locus_tag="IVO76_001252"
     CDS             22575..23297
                     /locus_tag="IVO76_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7429223.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
BASE COUNT         8067 a         3972 c         4852 g         6480 t
ORIGIN      
        1 tcaataacgt tcggacaaaa gatgggggaa cccatgaagc tggtatgaaa gcggcgatga
       61 cgaaatctta taatgaatat gcgagaaaag ttggtttgct gaaagaacgg gacaagaatc
      121 tggaaggtag tgatttcaga gaagggttag ctgctgtact ttctatccgt gtacctgaaa
      181 atcttctgca atttgaaggg caaacaaaag aaaaacttgg tacgccagtt gctcgtaccg
      241 tagtagataa cgtcatcagt gaacaaatgg gcttttattt gcaggaaaat agcgaaatga
      301 gccagatgtt ggtccgtaaa gcaatcaaag ccagagaagc gagagaagca gcacgtaaag
      361 ccagagaaga aagccgaaat gggaagaagc gtaaaaaagg cgaatctctt ctttcaggaa
      421 aattgacacc cgcacaatcc cgaaacccta agaaaaatga attgtattta gtcgaagggg
      481 attctgctgg cggatctgct aaacaaggaa gagacagaaa gttccaagca attctacctt
      541 tacgaggaaa agtcatcaat accgaaaaag ctaaaatgca agatatcttg aaaaatgaag
      601 aaatcaatac gatgatctat acgattggtg caggagtcgg tcctgagttt tctatcgaag
      661 actgtaatta cgacaaagta atcatcatga ccgatgcgga tacagacggc gcccatattc
      721 aagtattact gcttactttc ttttatcgtt atatgaaacc gctgattgaa gctgggaaag
      781 tatatatcgc gttaccgcct ttatataaag tatccaaagg acaaggaaag aagcaagtca
      841 ttgaatatgc gtggacagat gacgaattgg cagcaatgat taaaaaagtc ggcaaaggat
      901 atatgctgca gcgatataaa ggacttggag agatgaatgc ggaacaattg tgggaaacaa
      961 cgatggatcc gacttcccgt acgttgatcc gcgtccgtat cgatgatgct gcgcaagctg
     1021 aacgtcgagt aaccacactg atgggtgaca aagtagaacc tcgaagaaaa tggatcgaaa
     1081 atcacgtgca atttacgctc gaagaagatg gcagtatctt agataaaaaa gaggatactg
     1141 aaatctctcc ttcagtttct aatgatttat tagatgaaga acgagcagat aaaaatgaaa
     1201 acaatcagtt atttgaagta gaataggagg tcacctgatg gaaaatcgac aagaagtaca
     1261 agaattaact ttagaagaag tgatgggtga ccgcttcgga cgttattcca aatatattat
     1321 ccaagaacgt gcactgcctg atatccgtga tggattgaag ccagtgcaac gccggatatt
     1381 atttgccatg aacaaagatg gcaatacgta tgacaaagga ttccgtaaat cagcaaaatc
     1441 tgtcggaaat atcatgggga attatcatcc tcatggtgac atcagtattt atgaagcgat
     1501 ggtccgcatg agccaagact ggaagttgcg ggaagtactg atcgaaatgc acgggaataa
     1561 cgggagtatg gacggtgatc cacctgctgc gatgcgttat actgaagcca gattatccaa
     1621 gttaagtgga gagatgctag cagatatcga gaaagaaaca gtcgatctcg tgtggaattt
     1681 tgatgataca gaaaaagaac caacagtcct tccagctcga tacccaaacc ttctcgtcaa
     1741 tggatcgaca ggtatatctg ctggatatgc aacagaaatc ccgactcata acttagctga
     1801 agtgatcgat ggcacaatct atatgatcga tcatccacaa gcgagccttg aaaaattgat
     1861 ggaatatatt cctggacctg actttccaac aggagggatc ttgcaaggaa aagatgaaat
     1921 caaaaaagct tatgaaacag gacgaggaaa agttattctt cgttcgaaaa caaagataga
     1981 ggcaatgaag ggtaataaac agcaaatcgt gatcacagaa atcccatatg aagtaaataa
     2041 agcgaccttg gtgaaaaaaa tggatgagat ccgtttgaat aagaaaatcg atggaatagc
     2101 tgaagtacga gatgaaagtg atagaacagg tctgcaaatt gtcgtcgaat tgaaaaaaga
     2161 cgccaatgct caaggaattt tgaattattt gttcaaaaac actgagctac agatcaatta
     2221 caatttcaat atggtcgcta tcgaccatat gactccgcat caagttggac tgaaagacat
     2281 tttaagaagt tatatcgagc atcgaaagca agtcataaca aaacgtagtc aattcgattt
     2341 agccaaagct caaaaaagac agcatatcgt agaaggattg atgaaagctt tatcgattct
     2401 ggatgaagta atcgcaacga ttcgtgaaag taaagacaaa aaagacgcca aaaagaattt
     2461 agtcgacgtc tttcaattta cagaagaaca agcagaagca attgtcacat tgcaattata
     2521 ccgtttgacg aacacggata tcacagagct tcaaaaggaa tccgaaagct tgattgcgca
     2581 aatcacagag ttgaataaaa tcctttcaaa tgacaaagag cttttctctg ttatgaaaaa
     2641 agaattgcga gaagtgaaaa aaaattattc ttccgctcgt ctaacgacaa tcgaagacga
     2701 aatcgaagaa atcaaaattg atacgcaagt cttagtggca caagaagacg taattgtcag
     2761 tgtgacgcga gaaggctatg tgaagcgaac aagccttcgt tcttatagtg cttccaaacc
     2821 agaagaaatc gggatgcgag aaggcgacta cttgctatac tcaggtgaat tgagtacatt
     2881 ggaccatgtt cttctgatca caaataaagc aaatgtgatc taccgccctg tccatgaact
     2941 acccgatttg aaatggaaag atgctggtga gcacatttct caaacaatct ccaatctgtc
     3001 tgtagacgaa tcgattttag ctgttttccc atatcaaaaa attcaagcag agaaaacatt
     3061 tgtattcatc agcaagaatg gattgatcaa gcaaacacga atgacagagt tcgaaccatg
     3121 gcgcacctat aagagccgtc cgctcagctg tatgaagcta aaggcggaag aagatgaact
     3181 gattgctgtg tatctgaaag aagatcagcc aggcttagat gttttcttag taacgcatca
     3241 aggaatgggt ttgcgctatc ctttaacaaa tgttcccgtt gttggagcaa aggcagctgg
     3301 agtgaagtct atgaatttga aagatggaga taggattatc aacgggctgc ttgtgttagc
     3361 agaaggtgat acgccgatcg taatcgttac tcagcgagga gctgttaaac gcatgcttgc
     3421 tcaagaaatc agccaaactt cacgtgcgaa gcggggagtg acggtcatgc gtgagctgaa
     3481 aaaaaatccg caccgtatta tctatatgtc ggaaggaaga tcaaaagaaa taaccttgat
     3541 gaatcaaaaa gggcagcagt taacgattga cccaactgat ttccctatag gtgatcgaac
     3601 atcgaatgga tcatttgcga tggatgagaa aaaaggtgga gaagtcatcg aagttatcga
     3661 tgctcctgag attttcatcg agtcgtaact gttgtagtat tttaatgaag agacgccatc
     3721 tgtattaata caggtggcgt ctctttttca ttcattttta ataaatcctt atacgtttgc
     3781 aaaaaaagat gaaagtgttc acaatgctta aatgttggta tgtcaacgtt gatttaaata
     3841 tatgtaaata aaacataaaa attgttaaat aaaaaacaag aaaacccttg caaagatagg
     3901 gtgctgtgtt atactaatca cgtaaaaact gttacataaa acagaaattt aggaggtcca
     3961 tacaatggag caatggaatg gatttaaagg taccaaatgg aaagaaggcg tagacactag
     4021 agactttatt caaaataact acactgaata tcgcggtgat gacagctttt tggaaggccc
     4081 aacagaagca acagaaaaat tatgggatga attgcaaaag ttaaatgata tccaattcga
     4141 gcgtaacggt gtctatgata tggacacaga aatcgtatca actattactt cacacgatgc
     4201 aggctatttg gataaagatt tggaaaaaat cgtcggtcta cagacagaca aacctttgaa
     4261 acaagcattc atgccgttcg gcggaatcaa tatggctaac aatgcgctaa aaagcaatgg
     4321 ctatacacca aacgatgaat tgacacatat tttcactgac tggcgcaaaa cacataatca
     4381 aggtgtattt gacgcttata ctgctgaaat gcgtgctgct cgtaaaaaca aaatcattac
     4441 agggcttcca gacgcttacg gtcgcggacg tatcatcggt gactatcgtc gtgtggcatt
     4501 gtatggaatc gactatttga tggaacaaaa gaaaaaagat catgatatga ctggttatca
     4561 tacaatgagt gaagaagtga ttcgtttacg tgaagaaatc actgaacaat atcgtgcatt
     4621 agcgcaaatg aaagaaatgg ctgcgaaata tggctatgat atttctaaac cagctgcaaa
     4681 tgcaaaagaa gctgtccaat ggctatactt cggatattta gcagcaatca aatcacaaaa
     4741 tggtgctgcg atgtcaatcg gacgtatctc agcattcctt gatatctata tccaacgtga
     4801 tttggataat ggagtaatca cagaaaaaga agcacaagaa ttgatcgacc acttagtaat
     4861 gaaattacgt atggtcaaat tcgcgcgtac acctgaatat aaccaattat tctcaggatt
     4921 ccctatctgg gctactttat ccattgcagg tatgggatta gatggtcgtt cattagttac
     4981 aaagaatgat ttccgtctgc ttcatacatt aacaaatatg ggaccttcac cagaaccaaa
     5041 cttgactgtt ttgtattctg aacaccttcc agaaggattc agaacatatg ctagcagaat
     5101 cgctatcgaa agttcttcga ttcaattcga aaatgataat cagcttcgtg aacaatgggg
     5161 ttctgatgat tgtgcgattg cttgctgtgt atcaggaaca gttgtcggaa aagacatgca
     5221 attctttggc gctcgtgcta acttggctaa aacactatta tatgccatca atggcggagt
     5281 cgatgaagtg actggcgccc aagttggacc agaatatcgt ccaatcacat cagaaatact
     5341 agactttgaa gaagtgaaac ataacttcat gaatatgatg gattggttag cagaactata
     5401 tgtcaatacg ttgaacatca ttcattatat gcatgataaa tattcttatg aagcagctca
     5461 gctggctttg atggattctg acttgcaacg cacatttgct acaggtattg ctgggatctc
     5521 tcatgcggca gacagcttgt cagcgatcaa atatgcaaaa gtaaaagcaa tccgtaatga
     5581 aaaaggaata gcagttgatt ttgaagtgga tggcgacttc cctaaatacg gaaatgatga
     5641 tgaccgtgca gatgaattag tttcatggat cttggaatac ttcatgggac aaatcaaacg
     5701 tcataaaact taccgcaact caaaaccaac aacttcatta ttgacgatta cttcaaatgt
     5761 tgtttacggt aaaaatacag gaaatactcc tgatggccgt gaagctggaa aaccacttgc
     5821 gccaggagca aatccaagtt acaatgcgga acaaagtggg ttattagcta gcctgaactc
     5881 aacagctaaa atgaattacg gctgtgcatt ggatggaatt tcaaataccc aaacaatcaa
     5941 tccttcaggt cttggaaaag aacaagatac aagaatcgaa aacttgcgta atgtcttaga
     6001 tggatacttc tcaaaaggag cttatcattt gaatgtcaat gtattcagta atgatttatt
     6061 gcgtgatgca atggaaaatc caatgaaata tccgaacttg acgatccggg tatctggtta
     6121 tgcagttaaa ttccgtgact tgacaagaga acaacaatta gatgtcttga tgagaacttc
     6181 acacgacaga atgtaaaaag ttcgtgaaaa aagtttttcg acttagcgtc gaaaggacgt
     6241 tgaatagcca tcgcatcaaa atcgaataat cagtatgctt atttgaaaga aacgatttac
     6301 aggggcagga cttgtatgtt ccgtccctgt aaattttaca cataacgaag cggcaattgc
     6361 gaaaaatttt gttcagaaag cttcgcttcg acagctaact tgtacttaaa atgaaatcaa
     6421 agaaaagatg tgaacttaaa tggaagaaaa aacaatcggc tatgttcatt ctatcgaaac
     6481 tttcggttca gtagacggac ctggactgcg ctttgtcgtg tttatgcaag gatgccgcat
     6541 gcgttgccaa ttttgtcaca atccagatac ttggaatatt ggcggaggga aagaatacac
     6601 agcagatgaa cttttagata aagcagaacg cttcagacct tactggggag ataaaggtgg
     6661 tattactgtt agtggtggag aacctctatt gcagatcgat tttttgatcg aactatttaa
     6721 aaaagcaaaa gagcggaaga tgcatactac attggataca tgcggaaaac cttttacgta
     6781 tgaagaacct ttcttttcac gttttcaaga attgatgaag tatacagatt tactgttatt
     6841 tgacatcaaa cacatcgata atgaagagca taaaaaacta acgcattggg acaatgaaaa
     6901 tattttagaa atggctcaat atttgtcaaa aatcaacaaa ccggtttgga ttcgccatgt
     6961 actagttcct gagcgaagtg attatgatga atatttgatc cgactagaca actttattca
     7021 aaccttgtca aatgtagaca gagtagagat tcttccttat catacgatgg gaaaatacaa
     7081 gtgggaaaca cttggtttga aatatccatt agaagggatc gaaccaccta caaaagaacg
     7141 tgtcgaaaat gctaaacgat tgcttcatac agaagagtat acaaaatatt taacgcgata
     7201 ggtcgcatcg gaaagaagat aagtattgct tctttccgtt tttttgctat tattaaatag
     7261 gagaaatcac aaaggagcga atgacatgtc aaaaatttta gtttttggtc accaaaatcc
     7321 agatacagat gcaatcgggg cagccattgc gttttcacac ttgcaaaatg aattaggtaa
     7381 agaaacagaa gcagttgctc taggtacacc taatgaagaa acacaatacg cattagacca
     7441 ctttcagcta tctgcaccac gagtagtaga gtcagctaaa gcagaaaatg cagatgtcat
     7501 gcttgtggat cataatgaat tccaacaaag tatttcagat atcagcgaag taaatatttt
     7561 agctgtagta gaccaccatc gtattgcaaa ctttgaaaca gctaatccat tgtactatcg
     7621 tgcagaacca gttggatgta caagtacgat tattttaaaa ctatataaag aaaaccaagt
     7681 aacaattcct aaagaaatcg caggaatcat gctctcagct atcatttcag atacattgtt
     7741 gttcaaatca ccaacatgta cgcctgaaga tgaagcagct gcaaaagaac ttgcagcaat
     7801 cgctgaagtc gatctaaaca cttatggact tgaaatgttg aaagcaggaa caaatctaag
     7861 tgataaatca gctgcggttc ttcttgattt ggatgcaaaa agtttcccaa tgggcagttc
     7921 caacgttcgt atcgctcaag tcaatacagt ggaccttaat gaagtgatgg atcgacaagc
     7981 agagctagaa gcagcaatgg aagatgaaat gaaagcaaat ggctacgatc tgttcgtatt
     8041 agttgtcaca aacatcttag acagtgattc agaattactt gttgctggta aaggaaaaga
     8101 aaaagtagaa gaagctttcc aaacaacttt gaccaacaac cgtgctttgc ttaaaggtgt
     8161 cgtttcacgt aaaaaacaag tcgtacctca attgacggct gtatttagct aatcaaatgg
     8221 tagtatgaag cattcaaaat gaatggataa gcgattgata taaaaagaaa catagaacat
     8281 actaaaggcc gaagaaatgg aggagaataa cagtcaatta tgactcttgt tcttcctcct
     8341 ttttacgtaa aagcctttta agtaaaataa aacggataga aaagagaaca tatatgaact
     8401 attattcaaa aaataaggaa aacttttatc aaaaactgac cgatgatccg cttttttctt
     8461 cattgaccga ttatctatat gaacatcgag aacaagaaac gattctaaga gaattgaaaa
     8521 aagaattttc gcaaaataaa ttttcccatt ttcttgattt attgatcgat gcaggattaa
     8581 tcaagaggga agaacgaaga tatcatttga actttccaat atttgatcct aaagattact
     8641 tgcaacaagc cacttcggct gctgaaacca tagctgaaca attgaagaga ctctctgttg
     8701 atgaacaaaa attagcgatg ggagaagtta tttgggcgta ttgtttcgaa gatgaacgga
     8761 aagaagcgta tttttatgga gtaagaaaca gccgagagac agaattgctg aggacaactg
     8821 caggaaatga gaagtaccgt tttatcacac tttcctctat agaacacttc ccgcttactt
     8881 tggctaatta cttttttgta cagaagaatc agcttcctgt aacgaaggca ttcaaagaat
     8941 tagccgaact tattggagac gtaaatgaat cgtacttttt tgatcaaata gaagttatcg
     9001 ttgacaggat acgtaagaat aaatataaaa atcgccggcc tagtattttc catcagtctc
     9061 tacttgtgac gaatactata aaggaagaag aatcattcac attagaatta cctattgtcg
     9121 aaaaaaacaa ttttgagatt gaattaccaa cattggatcc ttcattgaca atggaggaaa
     9181 tggcctttct taaacgacaa atcttttctg agctgagcaa gaaatttata ccgcatgcat
     9241 tttcttatat caaagaatat ggaacagttt tagtgagtaa aacctaagag agcagtaagc
     9301 aaaaattata ctgactcttg caggaggagc ctttctcttc ctttatggtt aaaagaatta
     9361 tttgagtatt ttgcctatct accacctatt agattcacta catggtctgg aaacaaagga
     9421 atgcaaaaaa atggtgagaa atgtggaatg acaaaggaag tagtgataag aaaagttaga
     9481 tttttaaata atcaatatta tgattcagtc aagtatggca ttttatggga agaactagct
     9541 gattagatta ggaagtccat ttgataatcc agaatcacta agaaacgaat ggcgagtcat
     9601 taataattta tccaatcttg ggttattctt ttagattgaa gtgtttaaaa ctaaaaaagc
     9661 atttatgaag tcgtaatagt caggcttcaa aatgcttttt attttgtttg ttttttcttt
     9721 gccacataaa tacacctaga ctgacgatga agagcatatc caacagattt cctgcgactt
     9781 ggatcgtcga agggttggtc agtggcatta aagcaaggac aaaaaaggaa aacaagctgt
     9841 aaataaaaca ggtgacagga actaatcgaa agaagagcgg acatttcaat tggctaaaaa
     9901 tgagtgaaaa aataaaataa gtaaaaatgg ataaaatcac gactaaacca ttcatgataa
     9961 agaagaaaag cgtattgtga ttaaacgaat aaaaaagaat tgctaaaaaa gaaaacgttt
    10021 taaatgcaat caatacgctt gaagcttcgt ttaacgtacc aggatgtcga tgaaaaaaat
    10081 aggcaccgaa ataaacagaa aacagtaagc cgcaaagaga agacaaaatc aaaaaaaacg
    10141 gattttcttc ggcttctgtg atatcagaat gatccaaaag caaccagcct aatgcactag
    10201 aaggcgcatg tgaggtctta aggtatgaat aacatagaaa aaataagatc gtcaaagttg
    10261 caaaaaaaga gcctaaatgt aaaaaattag tggttctacg ttgaatttcc ataatatctc
    10321 caaattaaat tgataagtat ataaagtata ataacaaatt gatgagtata tgtcgatgtt
    10381 ttttaatcaa aataataaat aaaaactgtc taacatttaa aatttaattt agatattaga
    10441 cagtttttaa gtttgtgagt tattttcctt gtcgttttct cactttttta gtccagaaac
    10501 cacctgaaat atattttggc tcataagaaa tgataaaagc acgctcttcg atttctttga
    10561 tcaggcgata cagttcacgt tcgctttttc taggagaaag tatttcgaga actagccgct
    10621 caccttccag ccccattgca gaagtctgtg tcacaccata accgtgttct ctcagtatac
    10681 ccggcaaact tttatcttct gcgctatttg ctggcaaaat cgttgtgacc atgatgtatc
    10741 ctaatgccaa tttgtcttca atcaaaattc cgacactgat acctactgcg taacctaacg
    10801 catagacaaa taaattcagt ggattatcta aacgatctaa aacaagggat aagccgacga
    10861 tataaatcgt gatctcgacc atactgacaa gtggagcaat cagtcgatag cctttcattg
    10921 tcaacatgaa acgtaacgtg ttcagagtga tataagcaaa attaatgagg aagatcatcc
    10981 ccaacatttt tggatcaatc atttgtaaaa ctcctttcat ttatataaca acaatatcaa
    11041 aaaaaatcgt aagtgaagaa aaattgaaac tttatacata caaaaaaatg ataaaatgat
    11101 tataatagaa ttataaagat aggttaggtg attaaaatga aaacggtcaa tcgtgtgttt
    11161 tgtgccagcc acgtgctaag tctccttctc agtatatact taggtgtcta ttcgattcaa
    11221 cagccaattg aggtagagaa cattgttttt cagatgtcgc tgacagatgg tttaatttta
    11281 agcgtttttt tctttttgat actgtttatt ggtaatattt tcggtagcgt tgccagtttt
    11341 ttgaattttt ctatttatcc actcctttcg ctagcattag gagtggtggg actttgttct
    11401 ctgtgcttat ttcccgagcc attcagtcct gcatttatat tatttggtac actgaacctg
    11461 ttccagacaa ctgttggagc atggttgcta tggcgatcag gaaatttaat gaaaataggt
    11521 gagtaacaat tgaatccaat tatattgaaa cgttctgtcc atcatattcc agtgttagaa
    11581 gttgttctga aagaattaag agaggaacgt attcccgttg tcatctatta tcacggatgg
    11641 cagacaagta aggagttagt tttgacgcaa ggaagaaaaa ttgcgcagca gggcattcgt
    11701 gtgatcctgc cagatgcgat gaatcatggg gagcgcaaac agccggtttc ttctattcca
    11761 tcctatacat tttgggatag tatctacgga aatctgtttg aatttgacac acttatcaag
    11821 catttagaaa aaagaggtct tctacatgat cagcttgcag ttggcggggt ttcgatgggg
    11881 ggaatgacaa catgcgcctt gttggctaaa catcctcata tagtagcggg cgtgtgtttg
    11941 atgggttccc ctgcaccttt ggaatatgga aaagaaatcg taaggcgcgc atcggaatat
    12001 aattatgtcg taccaaaaga ctattttgat ttgatttcat gggttaagag ctatgatttg
    12061 tcattgcagc cggaaaagtt agctggacgt cctctgttct tttggcatgg acaagctgac
    12121 gaaaaaatcc cttatataca aacggaacga tttgtagaaa aaaatcggca taagtcttac
    12181 ggaaaagaaa ttgtttttcg ttcctcaaaa acacagcgac atatggtaca aataccacta
    12241 atggaagaag cttccacttt tttagcaaaa aatttgataa aaaagtataa ataattgaaa
    12301 cggaaaatca ctcatgacga tagaggattt tccgtttttt attttgggct ataataaact
    12361 ctgttaggaa aatttggtat aattgatagt aagaatgaca acatgttaga aaggaagaga
    12421 gcatgcccac tttagcagat gttgcaaaac gtgccaatgt ttcaaaaatg acggtctctc
    12481 gagtcatcaa tcatccggaa caggtgactg acgaattgaa agagttagtt ttctcagcaa
    12541 tggctgaact ggattaccgt ccaaatattg ctgcaaaagc acttgtcacg aatcggtcgc
    12601 agatcatcaa attatttata ctggaagaaa tcgacacaac tgagccctat tacatgaatt
    12661 tgctgatggg aatcgctaaa cggatcggta aagcgcatta ttctctccaa ttagttacaa
    12721 atgatgcctt cgacgtcggt tcttgtgatg gatacatcat cacaggtgtc agaaaaaaag
    12781 acttcgattg gatcgaccgt ttggaaaaac cagttgtctt gtttggggaa aattctgaag
    12841 gatatgatta tgtagacagc gataatgaat atggtacagc aaaagcaacg caatatgcct
    12901 tagaaagaga ttatcaaacg attatttata tcggtatcga agtggacgag ccttttgaaa
    12961 tttcaaggga aactggttat ttatctgtga tgaaggaagt tgatcttcct agtaaaatct
    13021 tgcggtttcc aaatcattcg acagaagtag aaagatatat gacagaaaac tgggcagatt
    13081 ttacagataa aacttgtttt gtatgtagtt cagatcggct agcactagga gcagtaagag
    13141 ggattataaa tgctggagga cacttgcccg aagaatttgg tgtgattgga tttgatgggg
    13201 tatttttaga ccaaatcagt tcacctaaat tgacaacgat caagcaagat attatacgta
    13261 tgggtgaagt atgtggagaa atgttgttaa aaaaaataga agaaaaaggg gcgagtcaag
    13321 gttatcgtcg tttttttcct gaactagtta tcagagagac aacgaattaa gtcttattac
    13381 tatatatata tttatgaaat tcatcgctat tttcttaaaa taatcagcgc attgctactg
    13441 aaaacaatga atctgtgcta ttattgagaa aagttccatt tttgagaagg atggtttgaa
    13501 tgaaagaaat cagacgaaaa catttccgtt ttcctgcgta cgatgatgag ttaggcgtaa
    13561 aattaaccaa cgacaagaag cgagtattat ttgacggaca agaagatctt ttgactgaac
    13621 agaatcagtc cccgtttgaa cagatgaaag aatttcattt cacgaacacg aacaccgaga
    13681 aaatccgtcg caagcgtcgt gcaacaaatg gattgaagtc agctccgcgt aaggaaaatt
    13741 tggaaaagca taaaggaaaa ttaccggatt ataccggcca ttaccaatca gaaatcacat
    13801 cgacaggacg aaaaaaactt tttggtgatt cctctcgttc tgtcagacaa agtgaaacaa
    13861 ggacagagtc caagcatact acacgaaacg catctgttga cacgaaggtg cgggagcgtt
    13921 cttattttgt tcctaaatat gtacccgcat cgattatccc agaccctaga gaaagcaaaa
    13981 tttctgagga tcatttaatc gaatcgatga aaaaaacgga tgactcttat ctgttgttcg
    14041 atacagaacc gacgccgtat caagaaaaaa gaaatggtga gccgtctgta cgaacattca
    14101 atcgaacaaa aacgttagag gaaacaaagc agtcagaaga aaaaaaatcg aaaaaaagcc
    14161 atggtatctt agaacgctca ttacgaggat tgatcgatga tcaaggaaac tctttgggag
    14221 aaaatagcta ttttaaataa gtgatataca aatttatatt cggctacagt tggaaaggaa
    14281 acgctgcggc gtttccttat tttatactct atccgaaagt ttatatcgta tcctcaaatt
    14341 tttttgagtt taagcgaatt ttttctccca ataagtaaga aaataatggc aaaataaaaa
    14401 aacaaactat tttcgcagat cgtttgcaaa aatagtttat ttttttataa agaagatttt
    14461 actgaataat atcattttag tttacataat gatatttctg taaattaaat aaagtagtga
    14521 aaaataagaa tcagtgctct aactgttcaa gaggatcaag tggttcaaat gtttctgtag
    14581 tctgttttgc ttccaattgg ggcaaaacag atccaatttc cagatacttc actttattca
    14641 atgtataaga tagaatagaa cagctaagta atgtatacag aaaatgtgtg agatccaaat
    14701 aaagcaagga gacaactaaa ataccaaaag taatcaagaa aagacttttg ccaatcttaa
    14761 tatgaaaata tttttcaatg gcttgataaa tgatatctgt tccgcctgtg cttccgccga
    14821 atcgaaagac gagaccgcaa ccaatgccag aaagaatacc tgtcatcaaa ctattgatca
    14881 gtagactatc tacagcaaaa tgtatatgaa ggctctccca taaatgtaaa aagaaaacta
    14941 gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt tccaaaaaga aacaatgaga
    15001 taagcaataa aggtaaatta aaaagcagtg aagtgacaga aggagcgaat ccaaaaagat
    15061 tatgcaaaag caaagcaata ccagtaaccc caccatctgc cagcttagat ggaatattga
    15121 ttcctgtcac agctaatccg tagattcctg cgcctaaaat gattccgcac aattctgtaa
    15181 ataaatttaa ttttttcatt tctagcctct tttcttttga aaattttcag caacttcttt
    15241 attatactga cttatactct tacttcaaga ggaaaatgaa aaaaaattat ttttggctgt
    15301 ttctttttat ttatcaaata aggagtggaa aaatgcgagc agcagactta ttgacaattc
    15361 ttacaaaatc acccaaaaaa acagagtatc aaggagtttt tttggctcaa gaacaaaaac
    15421 tggtgcctgt gacaatggtc cagataaata aagcaaatca atttatcttt ttatttgaac
    15481 acggcaaagc accacttgct atgaaagaca tctttctttt tttgatgaag aaccgtgaga
    15541 aagctgttta ttatcagtca aaagaagaac tcattcctct ttatggagtg aaagagatag
    15601 aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga cgtttatgat gtcaaaacaa
    15661 ccatttttta catttttggc aaaaaagtgt catgatacag tataatattc gttgaacagg
    15721 ccaataaagg tgttctcccc cacgagattt ctactttatt ggctgtttct ttttattaaa
    15781 ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa gtgacaattt cgtatagatt
    15841 tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt gatccgactt tgttgcttgg
    15901 attctttttt tgtcagcata tttagaggta tttttttctt ttttttgttc gtgctgtaag
    15961 gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat gtttatactt aaagaataac
    16021 agaaaggaag gtcgcatgaa taattttata caaacattaa gtgaaagaaa aggcgagttg
    16081 ctggaagctg catttcaaca tctgtccatt tctctcattt ctttgctgat tgcagcactg
    16141 attgctattc ctttagcgat ttgggcagcc aatcataaaa aaatggcaga agtcttattg
    16201 cagatcacgg gtgtgctcca aacgattcct tcacttgcct tattaggttt acttatccca
    16261 tttgtaggga tcggaactgt tccagcattg attgcacttg ttatttacgc tttgcttccg
    16321 attttccaaa acacgtatat tggtctagct gaaattgacc cgtctattga agaagcagcg
    16381 gtagcatttg gtatgtccag aatgagacgc ttgctgaaag tagagcttcc catcgcatta
    16441 cctgtcatta tctcaggtat ccgaactgcc ttagttttaa tcatcggtac ggctactttg
    16501 gcagcattga tcggagcagg tggattaggg acatttattt tgttaggtat tgaccgaaat
    16561 acacctgttt tgacattgat tggtgcgatc agttctgcct tgcttgcaat tatattcagc
    16621 ggactgattc gctggttgca gcataagaaa cctcgatatt ccatcattac tttagcagca
    16681 gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg ccttgtttaa aaatgaaacc
    16741 atcacgattg ctggaaaatt aggagcggaa cctgaaatcc tgatccaaat gtataaagaa
    16801 ttgattgaag acaacacaaa aacagaagtt gaattaaaac cgaattttgg caagacgagt
    16861 tttttgttca atgcactgga tcaaggtcaa atcgatatct atcctgagtt cacaggtact
    16921 gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa gtgctgattt gacaaaagaa
    16981 aaaacatatg acgaagcaga agaattattg aataagcaat tcgatatgac attattaaaa
    17041 ccaatggctt accaaaatac gtatgccttg gcagtcaaga gatcttttgc gaacgaacat
    17101 caacttaaaa agatttccga tttggcagct atctctgatc agatcaaagc gggattcaca
    17161 ctggaattta ttgatcgttc agatggatat aaaggaatcc aagaagcata tggcttgaat
    17221 ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc aagcaatcaa taatggagat
    17281 gtcaatattg tggatgccta ttccacagat agccagctca aggagtacga tctagtgact
    17341 ctagaagatg atcgagggtt attcccagct tatcaaggcg caccgctgat gaaaaaagat
    17401 tttgctaaag aacatcctga aatcgttaaa gcactgaata aattaagcgg aaaaataaca
    17461 gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg aaaagaaaaa gccgacagaa
    17521 gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg aggaagatca gtgactaaat
    17581 taatcgagtt caaacatgtg caaaaaaagt atgatggaaa gtatgtcatc gatgacctaa
    17641 atctggcaat caaaaaagga gaaatctttg ttttggttgg tccttccggt agtggaaaaa
    17701 caactacttt gaaaatgatc aatggattat ctaaaccatc agcaggagac atctacttca
    17761 aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg gtggaatatg ggatatgtgc
    17821 tgcagcaaat cgctttgttc ccgaccatga cagtcaagca aaatattgaa gtcatccctg
    17881 aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt agatgaactt cttcaaaaag
    17941 ttggcttatc tcctgatatt tatcgagatc gtatgcctag agagttgtct ggaggagaac
    18001 agcaacggat cgggatcatc cgagctattg ctgcaagccc agatgtgatt ttgatggacg
    18061 agccatttag cgcattagat ccgatttcaa gaaactcatt acaagaattg gttttgtcct
    18121 tacacgaaga attgggcacg accatcgttt tcgttacgca caatatggaa gaagcaatca
    18181 agctaggtga tcgtattgca tttatgaagg atggagaaat tatccaatgt gacacaccag
    18241 agcagttatt gatgaatcct aaaaacgact acgttcggca tttctttgat gaaccgaaac
    18301 agacgaaaga gtggcgagta gaagatttag tcgtcaatgg atatttctta aatgaaatac
    18361 cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat gaaagaagta tattcgcttc
    18421 tttcggtgta tccaagcatt gtgatagtag aaaagcaaag aagtattggg tcgctgacaa
    18481 gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg aaatgaaaac atatgatgca
    18541 atcatcatcg gttcaggtgt gagcggctta agtgctgctt atggattgaa agaagcaggt
    18601 aaaacagtac tagtggtcga agaagatcta tggggcggga cttgtcctaa ccgtggatgt
    18661 gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta atcgggtaaa acaattgagt
    18721 ggaaaaggtt ttaacgagat cccaactgcc aactgggaag aacttcaaaa attcaaacga
    18781 acatttacgg atccagtccc agaaagccga aaaaaacaat tagcagaagc tgaaatcgat
    18841 catctttcag gaaccgctcg ttttttggat gattcatcta ttgaagtcaa cgaagaagtc
    18901 ttccatgcgg attacttagt tttggcaacc gggcaaagac ctacgatctt accagtggag
    18961 ggaaaggaat acttgaaaac tagtgcagac tttttatcat tgcctgtact gcctaaagaa
    19021 atcattttta tcggtggtgg ttatattgct tttgagctag ctacgattgc caatgctgca
    19081 gggagcaaag taaccattgt ccatcataat caacggccat taaaagaatt tgaggcgtct
    19141 ttggtagagg aagccgtcca tcaaatggaa gcatctggca ttcagtttgc ttttggtgtt
    19201 gaaacccaaa agatcatttc agaaggtact cgctatcgtt tagtcggaaa agagacggaa
    19261 ttagtagcag atatgatttt ttgtgcgact gggagacagc cgaatacaga atctttagca
    19321 cttgagcaag cgaatatcgt ctttgataaa cacggaattg cagtcaatga ttatctgcaa
    19381 acgagcaatc caaaaatttt tgcgtgtgga gatattgttt caaggaaaac cccaaaattg
    19441 acaccagtcg ccacttttga aggaaattat gtagctaagc ggataacgga tgcaacaagc
    19501 gaacctatca aatatccgat tatacctacg atcgtctacg ccagtcctaa acttgctgaa
    19561 gtaggagtta caaaaagtca cgcctcttcg tcagatcaag tggtagagat ggatcttacc
    19621 agttggttca cctatcatcg tgtcaatgaa ccagttgcta aggctgaatt gacctttgac
    19681 cagcaaaatt atttgattgg tgctgcagtg attagcgagc aagcagacga attgatcgat
    19741 gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag agttggatag ctatatcatg
    19801 gggtatccga cattggcaag tgatctctcc tatttattga aataatcagc atgataacta
    19861 aagcgcttgt tgctttttcc ttcttgtgct acactttaaa aaatgaatac ctgtttctgg
    19921 ggatgtcgta gacgacatga gagtaagttg tgaacttaga cccattgaac ctgtcagtta
    19981 atactggtga agggagaaac atagcagacg cttgaagcgg gttactatgt cgtgcccatt
    20041 tcaagcgttt tttgttattc atattagaag aaaggaggta acaaaatgaa gaaaaatctt
    20101 tatccagcag tcagtatggc attcatttta attatttggc agattgttgt catagtttgg
    20161 aaaatacctg cctttatttt acccggacct gctgctgtat tttctgcatt agcggcagat
    20221 gcacaagtat tactttccca ttctgtcacg acattaaatg aagcagtgct tggtttggta
    20281 atagctagcg ttctttcttt tattactgcc ttggcaatgg attattggaa gtttttagaa
    20341 atgtcttttt atccgttact agtgatttca caaatgttgc caatcatggt gttagggcca
    20401 ttacttaccc tatggtttgg tttcggtatg cttccaaaag tgatccttgt tgtcttgata
    20461 agctattttc cgatcgtagt ttctttttcg gatacattga ggaaagtatc aaaagagcaa
    20521 atcatctttt tgaaaacgat gggggcagat acagggaaga tttatcgaat atataaaata
    20581 ccagcgggtt tatccggctt cttttccggt ttaaaagtgg ctgctactta ttgtgtcagc
    20641 ggagcagtcg ttggagaatg gctaagcgct caagcaggct tagggtatta catgatacgc
    20701 gtaaaaaaca gctaccagat cgataaagtt tttgctgcga tcatttgtgt cattctattg
    20761 agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag ggtattatcg tatgctttat
    20821 attttttaag agaaggggat caaatgaaaa aattatggct tttattaccg ctactgttgc
    20881 ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa acaagaaata aaggatttga
    20941 aaaaagtgac acttgttttg gattatgtac cgaatactaa tcatactggc atctacctag
    21001 caaaagaaaa aggctattat aaagaggctg gtttaaatgt ccaaatcatc gaaccaggag
    21061 ataattcgac aagtattggt ctagtaggtg cggacaaagc acaatttggt gtaagttatc
    21121 aagaagatgt cacttatgct cacgcagatg gacagaatat accagtcaaa gcaattgcta
    21181 cagtgatcaa acataatact tctggattcg ccaccctatc agacagtaac atccattcac
    21241 caaaagattt tgaaggaaaa acatatgcag gttggcaaag tccaagtgaa gaagctgtct
    21301 taaaagctgt gatggaaaaa gacggcgggg acttttcaaa actgacaatg gtaggaagca
    21361 atggagaagg gccagaaagc ttaggaaaat cctcagatat ccaatggtac tttgaaggtt
    21421 gggatatgat caaagcaaaa gaagcgggta ttgaactaaa ctatatccca ttgaaagaat
    21481 tagatgaacg gttagattac tatactccgg taattattac gaatgatcaa ctgatcaaaa
    21541 gtgatccaga attggtacag tcttttatgg atgccacgaa aaaaggctat caggaagcta
    21601 ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata tgcaaaagaa aatgatcgga
    21661 cgtttttaga agaatcacaa gcattcttgt caaaaaatta tacggatgat cctgagaatt
    21721 ggggattgat ggaagaaaaa gtttggcgca attatacagc ttttatgcag gaaaacggat
    21781 tgatcaagca agatgtacct tcgcaagaac tattcactaa tcagtttata aaataggagt
    21841 gatagatatg tcaatccaaa tcagccactt gaatgtagtc attgaaaacc tgaaaatatt
    21901 agaagacatc catttcacta tccctaaaga tacctttaca tcgattgtcg cgccaagcgg
    21961 agccggtaag tcaacattgc tgaaaacttt aacaggagtc cagcctatga cttctggaac
    22021 gatcaaagta gacgatcaaa aagtaactgg tctccataca gcatttagtt atatgccgca
    22081 agaagatatg ttacttcctt ggctgagcgt ctatcaaaac gttacgttat atcaaaaaat
    22141 caatcatcta acaatcaatg aagaacaagt aagggattat ctggatatct ttgggttaaa
    22201 cggctatgaa cattttttgc cggaacaatt atctggcggg atgaagcagc gaacggcctt
    22261 gttacgaaca atcatgaatc cttcttctta cttgcttctt gatgaaccat ttggagcgtt
    22321 agatgctatg accagagggc agatgcaaga ctggatgttg aagctgccta aaaaagcaaa
    22381 gcggactact ttacttgtga cgcatgatat cgaagaagca atttatttat cagacaggat
    22441 tcttgtgctt tccgcccgtc cggcacatgt gattgctgag attcaagtgc ctgaaaaacg
    22501 acggtcacgt gaatggctac tgcagcaatc agagttgaag caaacgattt atcagttatt
    22561 ggcaggtgaa ttagatgtta agtgatgctc attgtcattt agatgggagt cagagtttag
    22621 cactccttca gcaggaacac acgattttga cgatcatcaa ttgtgattcg ccagaagaat
    22681 ggaaggaaaa cagacagctt gcagcaagta agactcaagc cttaagttac ggtatccatc
    22741 cttggaaagc agatagctat acgtttgaac aagttgagcc ttttttgaaa aaagcaagga
    22801 tcatcggtga aatagggtta gataatattt ggacgaatgt accgatgact acacaaaaaa
    22861 aagtatttga gcgacagctt gcttttgcag ccataaatga gaaacctgtt gtactgcaca
    22921 caaaaggatg cgaaaaagag atcttaaatt atatccaagc ttaccctaat cactatttga
    22981 tccattggta ttcttctttg gactatcaac aagactatat tgatttaggc tgttatttca
    23041 gcattggtct tgatttgaaa aagaatccag cagtctggca actagctaaa gcagtcccaa
    23101 tcgaccactt acttattgaa acggatggac tacatgcagc agagtgggct ttagggggga
    23161 ttttttcaga aaaagattat gtctcactgc ttgaagaaca gttaaagaca atcgctgaga
    23221 tcaaagagct atcagttgaa gaagttaaaa aaaatactta tcaaaacttg gaacgtttca
    23281 ttagattagg taaataaaaa agagatgtga cacaactagt gtcgcacctc tttttggctt
    23341 tctttgtata aaagtaaact gtttgattaa t
//
LOCUS       DACCQI010000042        23274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCQI010000042 DACCQI010000000
VERSION     DACCQI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.042"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IVO76_001253"
     CDS             156..1862
                     /locus_tag="IVO76_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7429224.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IVO76_001254"
     CDS             complement(2020..2922)
                     /locus_tag="IVO76_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7429225.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IVO76_001255"
     CDS             complement(2919..3623)
                     /locus_tag="IVO76_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7429226.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IVO76_001256"
     CDS             complement(3654..4295)
                     /locus_tag="IVO76_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7429227.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="IVO76_001257"
     CDS             complement(4333..4647)
                     /locus_tag="IVO76_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429228.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /locus_tag="IVO76_001258"
     CDS             complement(4635..5264)
                     /locus_tag="IVO76_001258"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7429229.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IVO76_001259"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IVO76_001259"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7429230.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IVO76_001260"
     CDS             complement(5984..6916)
                     /locus_tag="IVO76_001260"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7429231.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IVO76_001261"
     CDS             complement(6913..7689)
                     /locus_tag="IVO76_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7429232.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IVO76_001262"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IVO76_001262"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7429233.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IVO76_001263"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IVO76_001263"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7429234.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="IVO76_001264"
     CDS             complement(12014..13294)
                     /locus_tag="IVO76_001264"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7429235.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="IVO76_001265"
     CDS             complement(13313..14239)
                     /locus_tag="IVO76_001265"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7429236.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="IVO76_001266"
     CDS             complement(14252..15535)
                     /locus_tag="IVO76_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7429237.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IVO76_001267"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="IVO76_001267"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7429238.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="IVO76_001268"
     CDS             complement(16323..17234)
                     /locus_tag="IVO76_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7429239.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="IVO76_001269"
     CDS             complement(17231..17695)
                     /locus_tag="IVO76_001269"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7429240.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="IVO76_001270"
     CDS             complement(17700..18398)
                     /locus_tag="IVO76_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7429241.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="IVO76_001271"
     CDS             complement(18385..18771)
                     /locus_tag="IVO76_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429242.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="IVO76_001272"
     CDS             complement(18918..20588)
                     /locus_tag="IVO76_001272"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7429243.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="IVO76_001273"
     CDS             20771..21004
                     /locus_tag="IVO76_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7429244.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="IVO76_001274"
     CDS             complement(21103..21534)
                     /locus_tag="IVO76_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7429245.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="IVO76_001275"
     CDS             21613..22026
                     /locus_tag="IVO76_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7429246.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="IVO76_001276"
     CDS             complement(22064..23212)
                     /locus_tag="IVO76_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7429247.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
BASE COUNT         6618 a         5162 c         4096 g         7398 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg tttt
//
LOCUS       DACCQI010000043        23163 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCQI010000043 DACCQI010000000
VERSION     DACCQI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.043"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..388
                     /locus_tag="IVO76_001277"
     CDS             <1..388
                     /locus_tag="IVO76_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HAP7429248.1"
                     /translation="EKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDPNTSPVV
                     MDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRKLFSEKR
                     EILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(410..1564)
                     /locus_tag="IVO76_001278"
     CDS             complement(410..1564)
                     /locus_tag="IVO76_001278"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7429249.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            1749..4190
                     /locus_tag="IVO76_001279"
     CDS             1749..4190
                     /locus_tag="IVO76_001279"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7429250.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(4520..5224)
                     /locus_tag="IVO76_001280"
     CDS             complement(4520..5224)
                     /locus_tag="IVO76_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429251.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(5411..5656)
                     /locus_tag="IVO76_001281"
     CDS             complement(5411..5656)
                     /locus_tag="IVO76_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429252.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(5673..6524)
                     /locus_tag="IVO76_001282"
     CDS             complement(5673..6524)
                     /locus_tag="IVO76_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7429253.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            6690..7694
                     /locus_tag="IVO76_001283"
     CDS             6690..7694
                     /locus_tag="IVO76_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7429254.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(7935..8291)
                     /locus_tag="IVO76_001284"
     CDS             complement(7935..8291)
                     /locus_tag="IVO76_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7429255.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            8659..9846
                     /gene="tuf"
                     /locus_tag="IVO76_001285"
     CDS             8659..9846
                     /gene="tuf"
                     /locus_tag="IVO76_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7429256.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(10034..12625)
                     /gene="mprF"
                     /locus_tag="IVO76_001286"
     CDS             complement(10034..12625)
                     /gene="mprF"
                     /locus_tag="IVO76_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7429257.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(12651..13370)
                     /locus_tag="IVO76_001287"
     CDS             complement(12651..13370)
                     /locus_tag="IVO76_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7429258.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            13579..14211
                     /locus_tag="IVO76_001288"
     CDS             13579..14211
                     /locus_tag="IVO76_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP7429259.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            14270..15130
                     /locus_tag="IVO76_001289"
     CDS             14270..15130
                     /locus_tag="IVO76_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7429260.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            15237..17630
                     /locus_tag="IVO76_001290"
     CDS             15237..17630
                     /locus_tag="IVO76_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7429261.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            17812..19653
                     /locus_tag="IVO76_001291"
     CDS             17812..19653
                     /locus_tag="IVO76_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7429262.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            19677..20786
                     /gene="rpoD"
                     /locus_tag="IVO76_001292"
     CDS             19677..20786
                     /gene="rpoD"
                     /locus_tag="IVO76_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7429263.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            21006..23105
                     /locus_tag="IVO76_001293"
     CDS             21006..23105
                     /locus_tag="IVO76_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7429264.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
BASE COUNT         7726 a         4116 c         4802 g         6519 t
ORIGIN      
        1 agaaaaattg atgaaaaagt caagcaaatg gtgcgtgata atcagagtag tggaatcaat
       61 cgatgatgtt ttttatccta tcgaaaagca actaggaatg atagaagaag atcctaatac
      121 atcgccagtt gtaatggatc aatttgaaac atacttaatt gatgaaggat tcaaagtgaa
      181 gaaaaaagat tttacttatt ttacacagga atcaattact gaagaattga tgaagctata
      241 ctatgacgcg tacgcaaaag atcctgttgt gaccaggtac attcgcaaac tattcagtga
      301 aaaacgagaa atccttagta cgactaagct gacctataga ctgctccttt ggtccgcaga
      361 taaaaaaaga acctttcacg actcgtgaag ggttcttttt atgattttat tatattttgt
      421 agtaacgaat atcattttcg atctttgtaa tcgaaaaagg tacatctgaa gtatattctg
      481 cacattctcc gtgttcagga agcgtctcac tgaaaatcgt ttcatattcg tcaacagtga
      541 tccgtgttcg agaatctagc atctcttgat gagattgtgc gaagagatat tcttgatagt
      601 tttcttccag ataacctgta aaaaattcac ttactgcacc agaaccgtag ctgaatagac
      661 cgatgcgatc tcctggctgt agtgatttcg aattttccag tagagaagtt agccccaggt
      721 ataatgaacc agtgtaaaga ttaccgattc gtcggctgta acggatgctt tcttcatagc
      781 gagccataag acgttcctga ttttcttcgt ctgtttggtc taatacgctt tgcaatgcct
      841 tttttcccat cttagtatac ggaatatgga aagcaatcgc ctgataatct tcgagtcctc
      901 gacccgacaa ttctttatgt cgattccaaa ctttttggaa tgattcgata tacgtagaat
      961 tagataaagg accatcaaca acaggaaatt cgctataatc tggacgccag aaatcataga
     1021 tatcttctgt cagaaatacg ctgtcgtctt caatcgataa aatacgcggg ttttgagtga
     1081 tcatcatcgc aacagcaccg acaccttgcg tcacttcacc accgcttgcc aagccgtaac
     1141 gagcaatatc acttgctatg actagtactt ttcgttctgg atgatttttg acatattctt
     1201 tcgccatgtg caaagctgcg gtgccaccgt agcaagcctc ttttacttca aaagaacgag
     1261 caaaagattg gattttcagt aaatggtgga taattaccgc actcgctttt gaatgatcaa
     1321 tcccggattc tgttccgacg atgaccatgt cgattagctc acggtctttt tctgttacga
     1381 ttttgctggc agcatttgct cctagtgtaa tgatgtcttc atttgcacga ttgactgcca
     1441 tttggtcttg accaatccct atatggtatt ttgcaggatc atccccacgg ctttctgcca
     1501 actctgtcat atctaaatat aaattaggaa taaaaaagga aagacgatca atccctattt
     1561 tcatttcaac acctctttac ttatgataaa aaacttactt tactaaggta tcataaaaag
     1621 attaaaagaa atataaaaaa gagaatattt aaggattgtt aaaatttaat cttagaaaaa
     1681 aataactgga acaagagcta tttgttttta cagcaaagaa tggtatattt ttgtaggagg
     1741 tggcttactt gaaagaagtc gttatgatag atgctgcaag aacgccgata ggaaagtatc
     1801 gcggaagttt aagtccattt actgcagttg aattaggcac acttgtgact aaaggattat
     1861 tagataaaac aaaactgaaa aaagataaaa tcgatcaagt aatatttgga aatgtcctgc
     1921 aagctggtaa tggtcaaaat gttgccagac agattgcttt gaacagcggt ctccctgtag
     1981 atgtcccagc aatgacgatc aatgaagttt gcggatctgg tatgaaagca gtgatcttag
     2041 cacgccagct gatccaatta ggtgaagcag aacttgtgat agccggcgga acagagagta
     2101 tgtctcaagc acctatgctg aaacagtatc agtcagaaac aaatgaatat ggtgaaccaa
     2161 tttccagtat ggtcaacgac ggattgactg acgcattttc aaatgcacat atgggattaa
     2221 ccgcagagaa tgttgcaaca caattttctg tgagcagaga agagcaggat cgctatgcct
     2281 tgtcgtccca gttgaaagca gcacatgctg tcgaagccgg tgtattttct gaggagatca
     2341 tcccagtcaa gatttctgat gaagacgtgt tatctgagga tgaagcagtt cgtggaaata
     2401 gtacattgga aaaactgggc acgttacgta cagtattctc agaagaagga actgtaacag
     2461 caggaaatgc ttccccgttg aatgacggtg cctctgtggt gatccttgca tccaaagaat
     2521 acgcagaaaa taataatctg ccttatttag caaccatcaa agaagtagcg gaggtcggta
     2581 ttgacccaag tatcatggga atcgctccaa tcaaagccat tcaaaaattg acagatagat
     2641 caggaatgaa tttatcaaca atcgatcttt ttgagatcaa tgaagcattt gcagcttctt
     2701 ctatcgtagt tagccaagaa ctgcagttag atgaagaaaa ggtcaatata tatggaggag
     2761 caatcgcatt aggccatcca attggtgcaa gcggtgcacg tatcttgact acattagctt
     2821 acggattgct tcgtgaacag aagagatacg gtattgcttc attatgtatt ggtggaggtt
     2881 taggcttggc agtcttgtta gaagcgaata tggagcaaac acataaggat gtgcaaaaaa
     2941 aaaagtttta tcagcttacg ccatctgaac ggcgcagtca attaatagaa aaaaatgtat
     3001 tgacacaaga aactgcgttg atttttcagg aacaaacact ttctgaagag ctctccgatc
     3061 acatgatcga aaaccaagtg agtgaagtgg aaatcccaat ggggatcgcg cagaattttc
     3121 aaatcaatgg caagaaaaaa tggattccga tggcaacaga ggaaccatct gtcattgcag
     3181 ctgcaagtaa tggtgcaaaa atctgtggaa atatctgcgc agaaacacct cagcggctga
     3241 tgcgagggca aatcgttcta tcaggaaaat cggagtatca agcagtgatc aatgcagtta
     3301 atcatcgaaa agaagagctg attctttgtg caaatgaaag ttatccttca atcgtgaaac
     3361 gcgggggcgg tgttcaagat atctctactc gggaattcat ggggagtttc catgcatact
     3421 tatctattga ttttttagtg gatgtaaaag atgcaatggg cgctaacatg atcaactcga
     3481 ttcttgagag tgtggcaaac aagttacggg agtggttccc tgaagaagag attctattca
     3541 gtattttaag taattttgct acagaatcat tggcttctgc ctgttgtgaa atcccattcg
     3601 aacgcttagg gagaaacaaa gaaatcggtg aacaaattgc caaaaaaatc caacaggcag
     3661 gggaatatgc gaagcttgac ccttaccgtg cagcaacgca caacaaaggg atcatgaatg
     3721 gtatagaagc tgtagtggca gctacaggaa atgatacacg tgcggttagc gcttcgatcc
     3781 acgcctacgc ggcaagaaat ggattatacc aaggattaac agactggcag atcaaagggg
     3841 ataaactagt tggaaaacta acggtgcctt tggctgttgc cacagtaggc ggagcttcca
     3901 atatcctgcc aaaagcaaaa gcatctttag ctatgttaga tattgattca gccaaggaac
     3961 tagcacaagt aattgctgca gtagggttag ctcaaaacct ggctgctttg cgtgcattag
     4021 ttacagaagg aatccaaaaa ggccacatgg gacttcaagc tcgttcatta gcgatctcaa
     4081 ttggggctat tggtgaggaa atcgagcaag ttgcgaaaaa attacgtgaa gcagaaaaga
     4141 tgaaccagca gacagctata caaatattgg aaaaaatccg ggaaaaataa aaaagcaata
     4201 tctttagaag aataaagcaa gatttacagt tattttattt actgcagatc ttgctttttt
     4261 ctctatcaga aaatatgttt attattagaa ttttttatag ttaaggttag gcatcttaag
     4321 aaatcaggca actccaagat atcttcaggt agaagagaag tcgcctaaca ttgtaggatc
     4381 ataggtgatt tgttggacag gtcaagctga tatttgagcc tgatgttaga aggaatttct
     4441 ttttacttgc tgtagaaatt attatatgac taaaaaggct gggattttaa atcccagcct
     4501 tttatgacaa atagaatttt tatcgatcag taatcgctgt cgctgcaaat tgttcgatca
     4561 aaggaatcaa agtcaatttg actttgtatt tccgttcgag tgctttttcg ataacgaact
     4621 cattttcagt agtcatagaa atagttgcta atttacgagg ttcatcagca tttttttcat
     4681 taataactac ttcatgttca ttttgttctg tgtaaaattt aggatactca gcaacggctt
     4741 ttgaaaaatc agcaaaactt aaagcggcat tcgtatcagt tgagcgtgta ttttcagttg
     4801 tgtccgcttt tggtttttcc gttttttctt ctggcacact gactgtttct tctgtaactg
     4861 gtgtcgcaac agcttgcgca gttggttcat cgtttttcaa tcgttgatca ttccagtaag
     4921 acacgatttc aataagaaga ttgtcacgga atcgttcgaa aatcccgttt tctcctgaac
     4981 gaattctctt taatacagta ttgaagttgt catcgattgg cacgttgatc cgtgtatctt
     5041 catttgtttt cccagctaat ccgatcgtta agatttgacg gtttttggga tcaagcatga
     5101 cttgtaacgg caaaccataa cgtcgcaagc tctcttcttc tagagaacgg aataattgtc
     5161 ctcttacacc atccaatgaa ggacggaatt gatttaataa tttttgtttc tgttttttct
     5221 tcatatgttc tctccttcgc tcataccctt tcgattataa gaaaaaaaca gccaaatgaa
     5281 aaggagtaat gcaaaaaaac ttgcgtatac gcaaattaca acagaaaaat ttgcgtattt
     5341 tcccgttttt tgaaagaaaa ataaaaaatg gaacggatta tgtatatata cataatccgt
     5401 tccattcttt ctattttttc ggtatcaagg caaaaacagg tagttcaggt gaataaatga
     5461 cttcatgttc gtgccagggt ttgaccatta gcgaatgatc gaaccgtttt ttcagttctt
     5521 cgatgatttg atcatgtgtc cagtttcttg caggaaaatg ttggattttc tctgcatcga
     5581 tcaaaaagac aaaatcggaa tgaaaagtct gggtaacttc ttccgagaga gaattcaagc
     5641 gttcgattgg atccataaaa tcacctttcg acttaaaatt gggtagtatc tgggtctttt
     5701 gctttgccga ctacaccatc taactgatcg atttgtttca tttcttcttc tgttaattca
     5761 aaatcaaata attcagtatt ttctttgatt ctgcttggtg tcactgactt aggaagagga
     5821 aggaattcgt gttgcagcga ccaacgcaaa gcaacttgtg caatcgtctt gtcatgtgct
     5881 tcagcgattt gtttcatttc aggcacatcg aagattttac cagtgcctaa tggactatat
     5941 gcttcgagga tcatatcatg tttttttgca tactcaacta cagttgtctg taattcgcct
     6001 ggtgcaagga aaacttgatt gaccatcggc ataatttttg ctgtttttgc tagtgtatct
     6061 agatggtggg gtaagaaatt gctcacaccg attgctttga ttttacctgc ttcatacagt
     6121 tcttcaaatg ctcgccatgt atctgcattt gcttgttccc aattatcacg aaaagcaaca
     6181 gggtttggcc agtggatcaa aaacagatct aaataatctg tttggagctt cttcaatgat
     6241 tcttcaaaag aagacatcac aagttcatag ctgtggtttg cattccataa ttttgttgtc
     6301 aagaatatct cttctctagg aattccactt tccttaatcg cttggccgac gctttcttca
     6361 ttcttgtatc cttgggcagt atcgatatgg cgataacctg cagccagcgc ttctttaaca
     6421 gaggaaacag cgacatcccc atcaggtgtt tgccatgtgc cgaatcctac taccgggatt
     6481 tcgtaaccat ttgatagacg ataagtgctt gttaatgaat tcatactttc agctcctttt
     6541 ttattcgttc aactataagt gtagcgcatt tttcgaaaaa aagaatccat tttgtttcta
     6601 ctcttgaaat ttttgtttaa aagaaaatga ttttgattct ggtatttttg attttttttc
     6661 cgtaaactaa gacagaaggg aatgagaaaa tggatatcta tttaaaaaaa gcaattctgc
     6721 atattattga cagagaaact ggttctccag tcttttcaca aaaagagctg gatctgacaa
     6781 aagaatatat tcgtgatttt cttcaaaaga agatccaaaa aatctcttct gctcaaacaa
     6841 aaactggaca actagcagaa gaaagcatga tcgttgaatc acttaaaatc gctcaagaaa
     6901 attttgtgga agcaagtgaa gcaatcgttc agcggtggtt cgatatctac caagaaagtg
     6961 aagaagcacc tagtgcagat gtatttgttg tcttgtatga attagataca gtgatgtact
     7021 tatctttatt gaaagtcaat tatcgtgagg cttacactca ctttgttgaa gcagacgaag
     7081 cagggatcga caaccgcttg attttgaatc gagcaattct aggggcgaaa acacaaaaag
     7141 cagatgaagc attcgctgtg aatttgactg attggaccta cgaattaatt gaaaaaaaat
     7201 atgagttttc tggcaaaaaa gagctttatt tttctggaag agtgatcgaa agtgttcctg
     7261 caccttcttt agaagaaaat gtaaaagtga tccaaaaagt agccaagcat cttggaaaaa
     7321 aatttgagac agaagaattt gatatcatgg ctgatttgaa agaagctgtc tatgatacga
     7381 tagaagaaaa aggtcggttg gatcatgaga tgattgcgga aaaagtcttc aaggaaaaca
     7441 tcacggcaaa aatggctttt aaagaagaag tacaagagca aggatttgtc ccagaagcac
     7501 ctcctgtaaa agaagtacaa gaaatttctg agaaaaaatt tggaaaacaa aaattcaagc
     7561 tatcaaacgg gatcgaactg atcgtaccag tagatgtata tcgcaaccca gatttgattg
     7621 aatttgtgaa taatccggat ggtacgatat ccgtgatgat caaaaatgtg gatgaagtat
     7681 tgaatcgttt ataaaaatga ctagaatatt aggtgttcaa gtaccagtga tcagttaaat
     7741 gaattgtgtc aagagaagga tcggaaaaga cgaatataac aaaaaaagag cgtgagacaa
     7801 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa
     7861 ctccttctta tttcttggag ttaaacgctt ctgtcccagc ctctttttat ctaataaaat
     7921 tttaagacca tttactaatt atcgattttt tctgccgcat ttttaggcac ttcagaagcc
     7981 tttatttctt cccagcttaa tacaccttta cgaggattga ctttgagttt aagatacgca
     8041 ttcatcttta atggatgttc tctttcttcg cgaagcgtca cctgtttttt ttcaccatct
     8101 ttattataag ccgcttgctt gtaggtataa gtcgtgaatt tctctccgtt atctgcttgg
     8161 gtagctgaca tttcgccatt cgtcgtgatt tttgtataat aagcttcgcc gccgtaaaaa
     8221 taatgataag ctgcaaaact tccgccgaga aatatgccta aaacgacaag tacgccaatt
     8281 aattttttca tgtacttcac ctctttcctt attattaacg aaatagaaga tttcgtctat
     8341 tgaaagttgt tggcaacagc tgaattttaa acttttcctc attttgttct tattgcttca
     8401 aaagaaagtt aaggactatc ttgaagtttt tctcaaaaaa cacatttttt tatttataaa
     8461 agatactttt ttcgcgtata agacagacga gtgctcttag ttgactttga ccaatattga
     8521 cgtataataa gcttagttcg aatcttacaa aaagtaagtc ggacgtattc tttttgattt
     8581 agtcaaaaaa agactataat aaaaaggtga gagctgaaat ttttcagtga tctatattat
     8641 ttaggaggtt tgtaaattat ggcaaaagaa cattatgaca gaagtaaacc acacgttaac
     8701 attggtacga ttggacacgt tgaccatggg aaaactactt taactgctgc gatcactaca
     8761 gtattaggca aaaaaggcct agcaaatcct caagactatg ctagtattga tgcagctcca
     8821 gaagaacgtg aacgtgggat tacgatcaat accgcacacg tagaatatga aacagaaaaa
     8881 cgccactacg cgcatattga tgcaccagga cacgcggact atgtgaaaaa catgattaca
     8941 ggggcagcac aaatggatgg tgcaatctta gttgtttctg caactgacgg tccgatgcct
     9001 caaacacgtg aacacatttt attgtcacgc caagttggtg taaaatacct gattgttttc
     9061 ttgaacaaag ttgatttagt cgatgatgaa gaattgatcg atttggtaga aatggaagtt
     9121 cgcgagttat tgagcgaata tggtttccca ggcgatgaca ctcctgtgat caaaggttcc
     9181 gcattaaaag cattgcaagg cgatccagat gctgaagctg ctattatgga attgatggat
     9241 acagtagatg aatatatccc aacaccagaa cgtgatacag ataaaccatt actattgcca
     9301 gtggaagacg tcttctcaat tacaggtcga ggaactgttg cctcaggtcg tattgatcgt
     9361 ggtgctgttc gtgtcggtga tgaggtagag atcgtaggga tcaaacctga aacacaaaaa
     9421 gcagttgtaa caggtgtaga aatgttccgt aaaacgttag attacgggga agctggggat
     9481 aacgtaggcg tgttgttacg ggggatccaa cgtgacgata tcgaacgtgg acaagtactt
     9541 gctaaaccag gttccattac tccacataca aaattcaaag cagaagtgta cgtgttgaca
     9601 aaagaagaag gtggacgtca tactccattc ttcaacaact atcgtccaca gttctacttc
     9661 cgcacaactg atgttacagg aacaatcaca ttgccagaag atacagaaat ggtcatgcct
     9721 ggtgacaacg tgacgattga tgttgacttg atccatccaa tcgctgttga aaacggaacg
     9781 acattctcaa ttcgtgaagg cggacggaca gtaggttcag gtatcgttac agagatcgaa
     9841 gcataagaaa cgcgactcga atattttggt aatgaaaaat gtttaaaata aaagagcggt
     9901 ccaaacagga tttctgtttg gaccgctctt tgttcttcaa ttgtataaga agagattgta
     9961 gaagcatgaa ctttcgtttt acttcagtct cttttattca tcatgagagt gttcatctat
    10021 cggtatgcag tttttaatac tttcttcgta tccatttttt caaaccactt attctacctt
    10081 gtgtctgctc aactggcgca ttatcaataa tcagcaaagc aatcataaca tatgcaatcc
    10141 aactatctct agaatataga gtataacgtg agacccagtt tgtagcgtac ttgcttttat
    10201 attctcttaa tccatggaaa gaataaaaat gagaaccaaa ctcgtaaacc aagtatgcaa
    10261 tcctttcctg taaaaaactc tttctggaag ttccgacatt tgaaagaggg gccatcccta
    10321 aattgaacca ttggatatgt tcttctttca tataggcaaa caaatgcaag aacaagaaat
    10381 ccatactgcc agaaggtgct tttttagaat catatcgcat cagatcaatc gttcctactt
    10441 tattgttcgt gtaagtaggc atgatcgttg caaaagcgac aatttcttct tgggtatttt
    10501 tgacaactgc aataggcgct ctttgtagat aatcttcaga gaaaaagcct aaagaaaacc
    10561 ctttttcctt tcgtgaaccc aaccaattat cagagatatt tttgaaagta ctcatttgct
    10621 cagtagaaaa tggtggttgt aagacatcaa atgtaaatcc ttctcgttcg atccgattca
    10681 agacagcacg agtgcccttc atttttttcc ctgaagttgt aaaactattc aggtctacat
    10741 aagcttcttc ccccatctta atgaaatcgt agccgaattc atgcaaaatc ataacaatct
    10801 cttcgcttgt ttcgtagaaa actggtaaat agcatagtcg atctgtttct tcgataaaag
    10861 cttcgattgc ttctggaaaa tcttctttct taccagaagg atcacccatt acaatacatt
    10921 tattattgaa gcaagcgaat tggagaagaa cagttggttc accgtctttt tcataggcaa
    10981 acatccgctt atcttttaag aagatcaatt gactatcgga gtttcctcca taagttgtaa
    11041 ggatcttcag ggccttttct tcattaaagg cttcgccaat ctgtttcttt tctccttgaa
    11101 ggaaatgaac aaatagaacg atcatgaaac taacagcgat gatggccaac aatcccgaaa
    11161 accaaatctt ttcagaagga aataagaaaa aggaaataaa atggtgacgc cgatgaggga
    11221 aatctggcag attgtatacc ccaataacga tgtaaagcaa ggacagtgca ccaataatga
    11281 ttccatcgat cgtacgccac tcccatgaat agatcaattg ttcccgaaat aattcgtttt
    11341 tagaagcaag aatgatcaga agcagaatag acaagaaaac caccggtgtg aaactaaaat
    11401 cacttaataa aacataaagt aaagctagaa caatcaagaa gattgtaggc aaatatgctc
    11461 gtttcacacg tgctgcgatc cctctgccca taatgattaa aagaaatccc aatagtatac
    11521 taggaaattg gataataaaa tgaaatttca atgggttcaa actatgaagc cagcgaaact
    11581 ctgtaaaagc ttgcgggata gtggcagaaa gtactagcat gattccagaa aagtataata
    11641 agacaaccac gattttatgc gcaatttctg ttgctaattg tttagggata ccagaataac
    11701 gctgatcaaa agtggaaccg atatttttga agaaaaagac gattccaatc aaaaacggaa
    11761 tgatgtagta gaacaggcga tagagcaaga tccacacaac gactatttct cttggcacac
    11821 caatagcgga caacccgata atcatcatta catcgaaact cccaatttct cctggaatca
    11881 tagacacgat tcctatcact gaagcagcaa tgaataatgg aattgtttgc cacagatcaa
    11941 tccggatttc cattaaataa ccaacagaaa ggaaactaaa caggacaccg gtccattcca
    12001 acaatgaaac tagcaatagc tgtccttgag ttttaagtga ggtaccacca aggaatcctt
    12061 tctttttgaa agtagtaaat aaaaagacga caggaaaata tagaccgccg ccgatcaacc
    12121 atatccaata ttgcttcaaa aaaggagcaa ctggcgtgaa gactacaagt aaaaaagaaa
    12181 gcagactgta aatagaaagt ccagccatca aaaacaagaa aactttcgat aatgcttgga
    12241 tcacgtttct gctctctttc ttttgtccat atagttcaga gcgaagcccc acactaacaa
    12301 acccgccgaa accggcaata ttgttgatcg tattgatcag ccagcttgtc tcaaacaggt
    12361 aacgaggatt ctgcttttgt tcaagcatcc gattcaaaat aatatcataa ccaaccatag
    12421 gtaacacaga gataagacca atcaatagca ttagtccgct tttccataca ggtatagtgg
    12481 caaacgtttc tcctaattgc tggatagata gagttttacc gatggacatc aattcaccga
    12541 caataatgac aatgacagaa atcaaaaaga ttgttttgaa cagtcctagg tggtttttca
    12601 gccattgtat ggtatttttt aacaaataaa tcaccttcta ttcctaatag ttatgtttca
    12661 gaaagaaatt gaagaacttc tcgattgaag cgttcaggat cttttctagc taattcatgt
    12721 ccttgttctt tcacaagcac aaaggaagct tttggtatag ttttagctat atacaaagaa
    12781 tgttttagct tgattacatc tttttttccc acgatgatca aagtggggga gttgattttt
    12841 tttaaatcat tttctgtaag cccgatgtca tgcagcaaaa gtttgataac taaaagattt
    12901 ttgcgaagac ttgggcgaaa taaagagagt aaccataccc atgcataatg aatattactg
    12961 atgactctgg cagaaaaacg aacgccttta acaagcgtgt ttccagaatt taaaatcaat
    13021 cgatgaactt ttttagggaa gctgctggca aataccagtg ctagatttgc tccatcacta
    13081 aatccaagaa aatctgcctg atcgattttt tccagcaaca tgatcgtatt cagatcctcg
    13141 gccattaact gaaaattcaa catagatgct tcgtttgtcg aacgaccatg tcctcggcta
    13201 tctactagat acacggtata gtagcgttcg agtacaggga cctgttctga aaaaaagcgg
    13261 ccgctgccgt cattgccatg cagtaaaaag agagggaagc cttgaccgct cttttcatag
    13321 taaatttcgc tcccatctgg catttttgca tatttctttt cttttttcac ataatcacct
    13381 actgtgtaaa gtataacgat tatatcagtc attgggcaat tgtttttact gaaaaacaac
    13441 gatcattttc gtaagatgtc aaaaaataca ggaaaattca ttttttctta aggcgtacac
    13501 agaaatcaag gaagaaatag ccaagaagat aaagaaatag taagataaaa aaagatgtca
    13561 aaataactag gtgatgaaat gaagcgaaaa cgattatcta ttatccgtat tcttttttac
    13621 atgatcattc tgacggttat tggtgcggtg ttgtttttag gaggaagaac ttatcgttcg
    13681 gtaaaacgag tggaaagcta tcaaatagaa ataacaacag ctgcaaaaaa ttatcatatg
    13741 gaaaactacg agaatttgat caaggcgata atattgaccg aatcaaaagg aaaagggaca
    13801 gatctcatgc agagctcgga aagtctgcat ggagaagctg agatcattga taatccagct
    13861 gacagtattg atcaaggtgt caaatatttg gcggaaatga tagtagaagc aaagaaacaa
    13921 ggatgcgatt tatggacagc tgtccaagca tataattttg ggaaagatta tattgcctat
    13981 gtaaaaaatc atggcggaga aaacacagta gaattagcag aagcgtattc taaagatgtg
    14041 ctgagcccac ttttaggaaa tgaggaaggg aaacaatacc gatattggcg gatccagtcc
    14101 ttgctttaca atggcggcta tctctatcat aatggaggta acatgtttta tcctgatatt
    14161 gtcaaaatga atgaaaaaat aatagagacc tatgaaaaag tctttaagta aaatagcagg
    14221 aaatgttcaa tcatgaataa aattaaatga aagaaggagt gcgcaaatag tggaaaaaag
    14281 gcaaaaaatg gaaaatcaag aagtggggta cacaaccaaa gactggatgt tacggtttgt
    14341 gaagggaatg tttatcggtt caggatttat cttgccagga gtcagcggtg gtgcattagc
    14401 agctgtcttt ggcatgtatg agcggctgat ttctttttta gcccacctta caaaaaattt
    14461 caaagaaaat gttttgtttt ttctgcctgt tggtctaggc ggagtgacag gcatctttct
    14521 actttctttt ttagtcagtt ttttattagg tgcgtatgaa acaacgatat tatggttttt
    14581 tgtcggatgt atcgctggaa ctgttcctgc gttatggaaa gaagcaggga aaaaaggaag
    14641 agatacttct gatgtgatca tcatggttgt tacttttgtt ctggcttatt tgtttttatt
    14701 atacggcgct caattatttg acgggcaagt cgatcagaat tttggaacat ggctgattgc
    14761 cggcggattg attggtttgg gtatgatcgt cccaggattg agcccttcta attttttggt
    14821 gtatatggga ttgtataaag cgatggctga cggatttaaa gaagtacgac ttgaagtgat
    14881 tgtgccgatc ggaattggtg ggattctttg tgttcttgga ttgtcaaaaa tcatggatta
    14941 cattttctca aaggcatata gcaagctatt ccatttcatt ttaggtgtag tttttgcttc
    15001 aacgattatg atcattccta ccgattacgc gggattggga gttgctgggg tactagcttg
    15061 tcctgttctt tttatagccg gtacagcact aggttggtgg atgagccgtt tggaggaaca
    15121 atataagtaa agatagattg ataataaaaa cagcacacgg gtgattcaaa caaaaaggtt
    15181 ggcatctttt ttgtaaaatc attcgtgtgt ttttctataa attttataaa aacaagttgt
    15241 ttttaatctt ggaacacgct agacttaaac acgtgattat ttttgaaagt tggcggaaaa
    15301 tggagaaaaa gaaaatgaat ggacttacac agcaagaggt agttcagaaa atggcttctg
    15361 gccagcaaaa tgactaccag gaagatgcat caaaaagtac aaaacaaata ttcagcgata
    15421 atatattgac gttattcaat tttctgaact ttggtattgg gatttgtctg ttatttgtag
    15481 gtgcgtattc caacatggcg tatttagcaa ttattgtcgt caatatcgtt attggaattt
    15541 atcaagaaat acatgcaaga aatcttgtac ggaaattatc gattgtttcc caatcagaag
    15601 tactagtgat ccgggacggt aaagaacaat cgatttctac taaagagctg gtattaggag
    15661 atttggtaaa aatcggagca ggagaacaga taccagccga tttacaggtt atatctggac
    15721 gggcagaagc aaatgaagct cttttgacag gcgaatctga tttgattgtc aaagaaactg
    15781 gagctgaatt gctttctgga agctttttga caagcggttc tgtcatggct gaagttatcc
    15841 gagtaggtgc agataattat gctgtccgct tgactagcga ggcaaagact cacaaagcta
    15901 ttcgttctga acttgtagat tcgattcgca aagtatccaa attcactagt ttcgtgatta
    15961 ttcctttggg gatcatacta tttttggaag cactgttcat ccgcgatgct tctatcaaaa
    16021 tttctgttat tgcatctgca gcagctttgt taggcatgct tccaaagggt ttggtattgc
    16081 taataagtat tgcattaacg acaggggtaa ccaaactggc aaaaaaacga atccttgttc
    16141 aagacatgta ttcaattgaa acgctggccc atgtcgatac attatgcttg gataaaacag
    16201 ggaccattac tgaagggaaa atgagtatcc agcgggtaga agcactacat ccgctgtacg
    16261 agaaagccat cccagaaatt atggggacgt atctggcaga aagcagtgat aacaacatca
    16321 ctatgcgggc tatgagagat tattttgagc tttctaatcg gtatgatgtg acaaacaaag
    16381 tcgcattttc ttctgatcgc aaatggggcg cgatggaatt tccagaactg ggagtcgtgt
    16441 acgtaggagc gcctgaacgt ttggtggaag aagatcggct tccggacagt ctaaaaacag
    16501 cccaagcgaa tggctatcgc gtacttatgt tggggatcgc tccaaatcag caattgaacg
    16561 aagaaaaacc agcatttgtt gagccggttg ctctgtttga aatcgatgat ccaatccgaa
    16621 aaaacgctca agaaacacta gcttatcttc atgaggaagg tgtggatctg aaagtgattt
    16681 ctggagacaa tccttttact gtctccaata tcgctagacg tgcaggatta ccgaattatg
    16741 atgcgtatgt cgatctttct caaattactg aagaaatgga agtgcgagaa gctgctcatc
    16801 gctatacggt ttttggacga gtgagtccgc agcaaaagaa attgctggta aatgaattga
    16861 aagaaagcgg cagaacggtt gcaatgacag gcgacggtgt gaatgatgtt ttggcgttaa
    16921 gagaagcaga ttgcagcatt gctatggcag aaggtgatgg agcaacacgt cagattgcga
    16981 atctggtatt gttggactct gattttacta cattgccaga agtcctgttt gaaggacgcc
    17041 gagtggtcaa caatgtgacg aaagtctcag gcatcttttt tatcaaaacg atttattcat
    17101 ttattttatc gattatatgt gccgttacag caattggatt tccgtttatc cctatccaaa
    17161 tcacgctgat tgatttagcg attgaagggt atccttcctt ctttttatct tttgaacaag
    17221 ataaaagaaa aatcacttac cgttatttgc ctacagcact tattaatgct ttaccaaatg
    17281 ccttgcttgt agtagtgaat atcattgcgg tgtatctact tggaaaaaca ttcgcatggg
    17341 gaaatgctga aactactact ttgatgtatt acttgctgat tgggatcagt tgcatggcag
    17401 ttgtaaaagc ttgttatccc ttcaacccat tgcgcatctt cctagcagta acgacgatcg
    17461 tagggatata tgtagctgca atgctgttcc atggaatttt ggaagtaaac ttagggttgg
    17521 gagagagcgt aggatacttc tttgtattca tgctcgtaaa tattgttctt cgagtattgt
    17581 actggaaggc tgatattcaa aatcagttac tgaaaagagt agcaaattga tgcttaatga
    17641 gatagaaatg tgttatactt gaaaaagcaa tgaaatatga tacaatcaaa catgtataat
    17701 tgtgtccttt aaaatcgcac tgatttcagt tagtgcgatt ttttaccggt tattctcctg
    17761 aaatatcttc tgttaaaccc aaaagatatc aatagagcag ggggtgaaaa aatggctcaa
    17821 cggattcctc aagaggttat cgaaacagta cgcagtcaaa cgaatatcgt tgaggtgatt
    17881 ggacaatatg tccagttgaa aaagtctggt aaaaattact tggggctgtg tccttttcat
    17941 gaagaacgta caccgtcttt ttctgttgca gaagacaagc agatttttca ttgctttggt
    18001 tgtggtaaag gcggaaacgt gtttactttt cttcaagaac tagaaggtct aagttttcca
    18061 gaagcagtgg taaaagtggc agacttcgaa cagataccgt tagaagatca atggcgtcag
    18121 cgtactatag ctgtccaaaa tcctgaatct gctacgggga aactgattgc tgctcatgaa
    18181 aaagcagcgg aaatctatca tcacatgcta ttgcatacaa aagctggaca gccagcttta
    18241 gaatatttgc aaagtcgtgg gttgtcttta gaattaattg aagaattcaa cataggtttt
    18301 gcacctaatg agcgttcttt tcttcagcaa gtattcaaga atgaatcaat ttcaaatgaa
    18361 ttgttagaac gctctggatt gtttgtcacg catgatgatg gccgtatgtc tgatcgtttt
    18421 tatcaaagag tgatgtttcc acttcgtaat tctcaaggcc agacgatcgg tttttcagga
    18481 cgctggcttg cacctgaaaa agggcaggaa aaagatcagc ccaaatactt gaatagtcca
    18541 gaaacagaac tgttcaataa aagacaagtt ttatttaatc ttgataaagc aagaagcgat
    18601 atccggaaaa acggagaaat tttgttgttc gaagggttta tggatgtgat tgctgcatgg
    18661 caggctggta tcaaaactgg attagcttct atggggacaa gtctaacagc tcaacagatc
    18721 caacaaatgg aaaagctgac gaaagaattg gtcttttgct atgacggaga caacgcaggt
    18781 tttgaagcaa ccaatcgagg gattgaattg cttcggcaaa acagtcgatt agcactttct
    18841 gtcgtagtcg ttccagaaaa gcttgatcct gatgaatatc tccgtaagta tggagaagca
    18901 tctttccaag aattagtggc tcatgggcgc gaaacagtct ttacttttaa atcacgttat
    18961 ttaaaacaag ggaaaaattt agataatgaa aaagacaaga tcgaatacct cgaagaatta
    19021 ctagttgaac ttagccgagt tgtttcacca atcgaacaag atatgtatct atctcaactg
    19081 tcaacagagt ttcagatcgg cagagagacg atacaaaaac aactgcgcga tcttcgaagg
    19141 acaaatcagc aaaatcgtca agaacctcag gaaagtcaac acactcaggt tacgagacaa
    19201 gaaactgcac ggaaaaagag acctttgacg caagtagaaa aggcagagca gattctatta
    19261 tatcgtgcat ttaaagaagt ttctgtccgt aatattttaa aagaacgaga atttcagttt
    19321 attcatgatg catatgcaga agtttatttt ttattcgatg catatgtctc acaacattat
    19381 gaattcaacc ttgcagagtt tttagacttt ttgcaagatg aaaatttaag gcggttggtg
    19441 gtggaaatcg aatatttacc ggtggcagaa gaaagttcgc cacgagaaat ccaagacttg
    19501 ctagatgtga tcagcaaatc tggcttagct gatgaaataa cattaaaaaa acaaatgcag
    19561 caagaagcca gacgaactgg aaacaaacag cttgagcttg aattgacgat tgaaatcatc
    19621 aatttaacga aacagttaaa acaagccaaa tagctactca aggggggcct tcttttatgg
    19681 caaatggaac agataaaaaa acgtatgaag cagcagtagc agctttcatc aaagaaaaca
    19741 aacccaaacg acaagttgta tatgatgatt tatccaatca gcttgctaca ccattcactt
    19801 tgaatgcaga tgaaatggac aaattgatcc aaaagattga agacgcaggt atcagtgtgg
    19861 tggatgaaaa tggtgatcca tcaattcaca gcttgaagag tgctgagaaa aaagctgaac
    19921 aagcaaaaac agaagatctt tctgcgccaa ccggtgtaaa aatcaatgac ccggttcgta
    19981 tgtatctgaa agaaattggc cgtgtctccc ttttaactgc ggaagaagaa gtagcgttag
    20041 cattgaaaat cgaagaaggc gaccaagaag cgaaacagcg tctggcagaa gcaaaccttc
    20101 gtttagtagt aagtatcgct aaacgttatg taggacgagg catgcagttc cttgacttga
    20161 tccaagaagg aaacatggga ctgatgaagg cagttgaaaa atttgactac cgcaaaggat
    20221 ttaagttttc aacctatgct acatggtgga ttcgtcaagc catcactcga gcaatcgctg
    20281 accaagcacg tacgatccgt attccagttc atatggttga aacaattaat aaattgatac
    20341 gtatccaacg tcaattgctg caagaccttg gaagagaacc tacgccagaa gaaatcggtg
    20401 cggaaatgga tcttccaaca gaaaaagtcc gagaaatctt gaaaatcgcg caagaacctg
    20461 tttcccttga aacgccaatc ggtgaagaag atgactctca cttaggtgat ttcatagaag
    20521 accaggaagc aacaagtcca gcagaacacg cagcttatga gttactaaaa gaacaattgg
    20581 aagatgtact agatacattg actgatcggg aagagaatgt ccttcgctta cgttttggtt
    20641 tagatgatgg ccggacgaga acattggaag aagtagggaa agtatttggt gttactcggg
    20701 aacgtattcg tcagatcgag gctaaagcat taagaaaatt gcgccatcct tctcgttcaa
    20761 aacaattaaa agatttctta gaataagcgt ggactattga ccagaagaga aatcagtaaa
    20821 actgtttggt ttattttaaa agaaaaaaag cttgagacta cgcagtctca agcttttttt
    20881 tctataggaa ggaaattcat tcattatttt taagtaaatc ctaaaaataa gctattggcg
    20941 tgacgtatag aatatgatag attaaatggt agcgtaaacc tagtaaagga gccgatcgct
    21001 aagtgatgaa aaaatgtcca aattgcggat cgaaaaaaat ggagcagaat actatctgtc
    21061 cagactgcgg tcatcaaata actgcagaag atcagcaaat aaaagaatca attgaagaaa
    21121 caaatacaga tgcagctatg caggataaat caactgaaga tgcagtcttt cctgatacag
    21181 aattaaacga tccaatcgaa tggtcggaat tgaaagattt acctctagaa tctgtaatgg
    21241 aattattcga aaaagcagaa ccagaagacg aagaaccacc tgctcacgat caagtaacta
    21301 atatagagaa aaatgaacag aagaaagcaa ctgttcatga gacttcggaa gaatcaggaa
    21361 agaatcaaga agaaacgctt gaatcaaaac aaaaaagaag agtagctgaa ctgaaagaaa
    21421 tcgtcgataa tgaggaagaa aattcgatac tatccgctta tatcaaagca catcgtgaag
    21481 atacaaaaga agagcacgcg aaagagctgc tcaaaatgat cagtgaaaaa attgcacagg
    21541 aaaacaaagt agagccagaa aaggaacaag aaactgaaaa agactctgat agagaaaacg
    21601 ccacaggtaa agaaacagta aaaataaaag acgtaaaaac ggatatcaca gaaaaaactg
    21661 aagtatttac tgaaaaacaa ttagaaaagg aaaaaacgaa taaactgccg gaagcagaga
    21721 aaaaagaaat actcgaaaac acagacaaaa agtcaacaga gcccgatgaa ataaatagtg
    21781 aatccgatgc tgaaaatcaa tctgtaagca aagagggtac tgatcttgaa gcaaaaatag
    21841 aaaaagaaga gaaccagcct aagctgtttg aagcagaagc gaaatcaata acaatcgtgc
    21901 cagatactga aaaaacacat caagaatcaa acttagaaaa aacagatcca gataaagcgc
    21961 aaaagaaaaa atcaaaaaaa gtaccttatc tcgttttagc agcagtactt ctactcggtg
    22021 gaggaagctg ggcttattat gaccatcatc aaaaagtaga agccgaagta gcagcagaag
    22081 caaaaagaca gcaacaaatg gaagaacttc atgagaaact tgctgccttt tatacagatg
    22141 atcaccatca atttattcgt gcagatatga ttcatcaaga tttatcaaaa ctaaaaaatg
    22201 atttatccag tcttaagaat gaaaaaggat attcagagtt agaaacaact taccaagata
    22261 tccaatctaa gatccgagat atccaaaaag tgaatgagtg gttcatagca cctgccatcg
    22321 tcgatgatca tctagcagat gacatcaagt tgaaagcaga taaagagatc caagcaatcg
    22381 aaacggataa cacagatttt ggaaaattga ttcaacaagc acaaacagaa gctaaaaatc
    22441 aatatgctga attgcaaaca gctaaagaaa aaacagctat agtctttaaa gacggaaaag
    22501 tagtggattc agcaacacgc gaccaataca agacggcaaa agaagcagta gaaaaagtga
    22561 aaaatacaga tctatctgct cagttgaatg aacaattgaa acaagtagat aagatgctca
    22621 ctgataaaga gaaaaaagaa gaagaggaaa agaaaaaagc agaagaagcg aaaaaagcag
    22681 ctgaagaaca agcaaaacaa gccgctcaag cagcagctgc agctcaagcc gctcaacaag
    22741 ctgcggtcgc acaagcagca cagcaagaag cggcatcctc gaccccttcc tcttcaaact
    22801 cagcgaatca gccaatcaaa tctgccagag caagtgatat tgccgatgca tctaatccag
    22861 catggacgtg ggcaccaggt gtccaaagca aagtgattgc tacatgtatc caacgtggat
    22921 atatcgttga aggaggttac agattagaaa aagccagaat cgaaaatggg gaaggctatt
    22981 ataatctata tgccacttca acaaaatctg cattgatgaa aggcattgga gaaagtgctt
    23041 tgccatttta catcgtaacc atcaactgta aaactggatg gttcggagga aatgggagca
    23101 attaatttta ggcaaatgaa agaggctgtg agcctgccgt ttaacaccgt ttattcgaaa
    23161 aag
//
LOCUS       DACCQI010000044        22433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCQI010000044 DACCQI010000000
VERSION     DACCQI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.044"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(12..1388)
                     /locus_tag="IVO76_001294"
     CDS             complement(12..1388)
                     /locus_tag="IVO76_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7429265.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(1516..2634)
                     /locus_tag="IVO76_001295"
     CDS             complement(1516..2634)
                     /locus_tag="IVO76_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7429266.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            2776..3042
                     /locus_tag="IVO76_001296"
     CDS             2776..3042
                     /locus_tag="IVO76_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7429267.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGMPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILLKMLACLIFI"
     gene            complement(3134..3667)
                     /locus_tag="IVO76_001297"
     CDS             complement(3134..3667)
                     /locus_tag="IVO76_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7429268.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(3761..4939)
                     /gene="mutY"
                     /locus_tag="IVO76_001298"
     CDS             complement(3761..4939)
                     /gene="mutY"
                     /locus_tag="IVO76_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7429269.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(4932..5729)
                     /gene="recX"
                     /locus_tag="IVO76_001299"
     CDS             complement(4932..5729)
                     /gene="recX"
                     /locus_tag="IVO76_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7429270.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            5836..7218
                     /gene="rlmD"
                     /locus_tag="IVO76_001300"
     CDS             5836..7218
                     /gene="rlmD"
                     /locus_tag="IVO76_001300"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7429271.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(7392..7970)
                     /locus_tag="IVO76_001301"
     CDS             complement(7392..7970)
                     /locus_tag="IVO76_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7429272.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            8292..8762
                     /locus_tag="IVO76_001302"
     CDS             8292..8762
                     /locus_tag="IVO76_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7429273.1"
                     /translation="MKMIKDIRFWVCGIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(8870..10096)
                     /locus_tag="IVO76_001303"
     CDS             complement(8870..10096)
                     /locus_tag="IVO76_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7429274.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYIPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(10850..11050)
                     /locus_tag="IVO76_001304"
     CDS             complement(10850..11050)
                     /locus_tag="IVO76_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7429275.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(11184..11792)
                     /locus_tag="IVO76_001305"
     CDS             complement(11184..11792)
                     /locus_tag="IVO76_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7429276.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(11877..12347)
                     /locus_tag="IVO76_001306"
     CDS             complement(11877..12347)
                     /locus_tag="IVO76_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7429277.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(12395..13309)
                     /locus_tag="IVO76_001307"
     CDS             complement(12395..13309)
                     /locus_tag="IVO76_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7429278.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            13455..14258
                     /locus_tag="IVO76_001308"
     CDS             13455..14258
                     /locus_tag="IVO76_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7429279.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            14436..15383
                     /locus_tag="IVO76_001309"
     CDS             14436..15383
                     /locus_tag="IVO76_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7429280.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(15451..15744)
                     /locus_tag="IVO76_001310"
     CDS             complement(15451..15744)
                     /locus_tag="IVO76_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7429281.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(15772..16437)
                     /locus_tag="IVO76_001311"
     CDS             complement(15772..16437)
                     /locus_tag="IVO76_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7429282.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(16577..17209)
                     /locus_tag="IVO76_001312"
     CDS             complement(16577..17209)
                     /locus_tag="IVO76_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7429283.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(17216..18283)
                     /locus_tag="IVO76_001313"
     CDS             complement(17216..18283)
                     /locus_tag="IVO76_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7429284.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(18280..19011)
                     /locus_tag="IVO76_001314"
     CDS             complement(18280..19011)
                     /locus_tag="IVO76_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7429285.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(19179..19658)
                     /gene="greA"
                     /locus_tag="IVO76_001315"
     CDS             complement(19179..19658)
                     /gene="greA"
                     /locus_tag="IVO76_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7429286.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(19794..20969)
                     /gene="mltG"
                     /locus_tag="IVO76_001316"
     CDS             complement(19794..20969)
                     /gene="mltG"
                     /locus_tag="IVO76_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7429287.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            21134..22294
                     /locus_tag="IVO76_001317"
     CDS             21134..22294
                     /locus_tag="IVO76_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7429288.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         6767 a         4543 c         3603 g         7520 t
ORIGIN      
        1 caagtttcca attatcttct tggctcgtca tcttcgaatt cttttctgaa gtatcttcgt
       61 tcttcttcag gttccgcttc tgtttcttcc ggatcatgaa aaatgacacg gattttaagg
      121 attcgagaac cttccatttc ttcagaaacc agcgtaatat tatcgacatc aaacgataac
      181 ttttcgcctt catccggaat catgcctaat gctgtaatca aataaccagc catcgtatct
      241 acatcactca tatgcagatc actgtcaaaa gcttcattga actcatcaat cagcatacgt
      301 ccttggatga tatattcgtg ttcatcgatt ttttcataaa gattctcaac ttcatctgat
      361 tcatcatcga tttctccgac gatttcttct aaaaggtctt ctagtgttgc taacccaacg
      421 acgccgccat attcatctaa taaaatagcc atctgattct gcgtcttttt catttcatat
      481 aacagatcat caataaagac cgtttcagga acgaataaag gctcctgcat gattttcttg
      541 atatctaaat tatcaaaacc gaatttatgg gcagctttta gtaaattctt tgtatgaagg
      601 attccgacta ctttatcttt gtcttcgtta taaacaggga ttcttgaata gttttcggat
      661 aaaacttcat tcacattttc ttccacagtg tcgttgatat caatcataaa tgcatccgtt
      721 cgtgggacca tgacttctcg ggcaactttc gtatcaagag aaaaaacccc ttggagcatt
      781 tctaattctt cattattcaa tacgccttcg ttttcaagca tataacgcat ttcatctctg
      841 gtcatttttg aatcttcatc atcaaaagtc atcggcgtga tacgcgccaa taaacttgtg
      901 gaagcagaaa gcagccagac gaagggctta gctactacac caatcaagcg tatcagccct
      961 gaagtgaact ttgcgacttc ttctgattta tttaaagcaa tacgtttagg gtacaattct
     1021 ccaaaaacaa tcgatacata ggtcaaaata gccagcacaa tgatatttgc aatatttttt
     1081 gccgctgtgc cgccacctaa taccggagct aaccgttccg ataaagtatt agctagtgaa
     1141 gcaccagaca aaatgttgac caaagtgatt cctacttgga ttgtcgatag gaaattattt
     1201 ggattctgca atatttttaa aagcttttga gatttcacat ctccttcatc agctttttgc
     1261 tcgattcgat tcttattgat cgaaacaaca gcaatttcgg aagctgctaa aaaagcattg
     1321 atcaatgtca aaacgatcaa taataaaatc tgcgctaaca gcgactgact ctcagggtca
     1381 gcattcataa tcgcattctc tccttataaa attaattttt agtttacgat agaaaaaaac
     1441 gtggcactat agccacgtta aaatggtatc acaaagcaat tttctttgca ataaaaacaa
     1501 gtgtactaaa actacctacg aggtcgtagc aattttcttc ttgttttcat tttgcttgtc
     1561 ttctttgacg attttgatta tataggaaat gatcgtcttg cagattgcgt aaaaaggaac
     1621 acctaagaag ataccaagca atccggcaat atttccagct actagaagaa cgataatgat
     1681 cgttaatgga tggattttca gtgatttccc aattacatta ggataaatga tattcccatc
     1741 caactgttgg acgattagca caaccacaca acaaagtaat gcttttaccg gatcattgaa
     1801 gactgttact agaaatgcag gtgctagtcc tagataaggt cctaaatatg gaattaggtt
     1861 tgtcaatccc gcaatgaccc caaataagaa agcataatcc acaccaatta ccatatatcc
     1921 taaaaatgtg aaggtaccaa cgaataaaca ttcgattgct tgtccgctaa tgtaattagc
     1981 caatgtttta ttcagttgcc ccaataactc cacaatttgg tttcttcttt tttctgggaa
     2041 aaactgttgg atgtttggca ctagtttttc tccgtctttc aacatataga acaagatgaa
     2101 tggtgctgta atgattacta cggttgccga agcgatcgtt ccaacgatcg aacctaaact
     2161 attggatact ccactaagga attgctgaat gattgtcccg taagaaatgt ctaattgctc
     2221 aaaatattta ttgatatcta cttccttgaa caaaggcaat tcggctacct cttttaacca
     2281 gctttctacc tgtttgatga aatcaggcat attagatgcc aatgaggaaa tttgctgtac
     2341 tagacttggg atgacactaa gtaaaatcca aataattgct gcaactaaaa ggagcagcac
     2401 aattgcgaca gcccatattc ttttgatttt tgctttcatc aacagcttga caataggatt
     2461 caataaatag taaagaaagc ctgcaatcag tacaggtgca aataacgtgg aaaaaaatgt
     2521 cccgatcggt tcgaaaataa agtttatttc tgatgaaatc atgatcaatg cagctatgat
     2581 caataattca agcgaccaaa acattagttt agactgtcta agtttttcaa acaatagatt
     2641 tcctcctttc tttcttacta gtatatatta tattcgttaa aaattctttt attctttttt
     2701 tatttttgaa aacttgccat actttttatt tgttttattt tccagtatac tatacgaaag
     2761 gaaggtggct agaccatgaa agttgtacaa acaaaagata caatgagcaa tatttactta
     2821 gatgctgtac ggatcaggca tcaagttttt gtagtcgaac agggtatgcc tttgtctcga
     2881 gaaatagaca aagatgaagc ccattgtatc cacttcgtcc tgtactcaga taaaaaagaa
     2941 ccgcaaggaa ctgtacgtct tttgccttta gaaaacggaa aaatgaaatt acagcggatg
     3001 gcgatccttt tgaagatgct ggcatgcctc atatttatat gaaaaagact ttataaaaat
     3061 ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa aatctctcac tggcttactg
     3121 ttcttttttg tgtttactta cgcgacaatt gcttgtaacg gttataccaa atatcaatat
     3181 agccttccga gaacgggcca tctccattat tgatccagtc aaccagaatc ttcacatttt
     3241 cttttaggat gaagtcaata tcgttcggat aattcatcat tttgctgtgc atctcatatt
     3301 catcgacatc caatagtcgt ttctcgccgt caggaaatac cttgatatcc aagtcatagt
     3361 cgatatattt caaggcctca tcgtccagca aaaaaggtga agctagatta caataatagg
     3421 aaaccccctt ttctctgatc attgctatta cattgaacca gtatttttta tgaaaataaa
     3481 caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga ttcagtgact aatgtgtgat
     3541 cattcacgcc gatcatagag tgttcgcttg tttttaatac catggtatct cgccacgttc
     3601 gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac aaactctcct tcttttggaa
     3661 ctcccatagc aacccttcct taagcagcat tttgtgcttt gtgtttgggt gttcattaca
     3721 ccgacatcat aattattata tcaataaaaa tgagctttgc ctagaaagga ttcctattgt
     3781 ttcgattttt ctctaactga tctaccagtt tcatttggac cttaggaaaa acgaccgaat
     3841 caaatgcatc aggcttcaac cacttcgaag ttttgttctc ggtaaattct tgttctgctc
     3901 cctcggttgc ccgtccgtaa aaaaggagaa catgccattt cagatgacta aaaatatgag
     3961 taacttctcc taaatgtcgt gtctgccaaa caaagagttc ctgcttttca aatgggagat
     4021 tttgctcttc ttctgctact aggtcatcaa acagatcgat ttctggcttt gtttcccatt
     4081 cttttttcaa tcgctcatac tcctcttgtg tcacttccat cattggaaat gtccacatat
     4141 tcgccagcaa tttttggcta tctctttctt caaaataata cgctccagaa tgattttgaa
     4201 gagccgcact gatataataa acgtcttttg gctttgcttt ttttgttttt acaggaaaac
     4261 tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg gatcggacaa gtctcacact
     4321 ttggagaagt tggtgtacag atagctgaac ctagatccat cattgcttgg ttgaattcac
     4381 ctggatgctt ttcatcaatg attttccgca tcgcttcatc aaagattttc ctgctagaag
     4441 cttttgcgat gtctgcttca atgcaaaaca atctgctgac tactcgcatc acgttgccgt
     4501 caaccgcggg ttctggaagt ccaaatgcga tacttgcaat cgcgcctgtc gtatatggtc
     4561 ctattccctt caatgagcta atttcttcag gcgtttgcgg catttcccca tcaaattctg
     4621 acatgatctg tttagcagca gcttggatgt tccgggctct tgagtaataa ccaaggccct
     4681 cccatgcttt taaaagcttt tcttctggag catttgctag ctcttcaatt gtaggaaacc
     4741 attccatgaa acgataaaaa taatcgatca ctgtgtctac tctggtttgc tgaagcatga
     4801 tctcagagat ccagatacga taaggatcac gattatagcg ccaaggcagg tttctttttt
     4861 cttgttcata ccattgaatg aactgatctt gaaattcttt tgtttcttta tctgtccatt
     4921 tttcccattc tttattcact ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc
     4981 aaatcaaatc ctttttgata taagctttgc tttactttta gatttctttt tttaggttca
     5041 aaccttaaat ttcttcgcca tagtttatct ccttgtttga ctaaataatc atattctatt
     5101 tcttgatctg cttcttgcgg aagatcagcc atcgcttgtt ggatcacttc ttgagaaaaa
     5161 cctttcgtca tcaatgtttg ttggattttt ctcaataatt ctttttggct ttttccgcga
     5221 ttttttccaa acgctttttc ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta
     5281 tattggctga gcgcttgttc aatgatttca ggtttcaaac ctttttgctg catctgctgc
     5341 gctaatttct ttggtccttt gtcacttaag cgcatattcg tgcgcacata actttcccca
     5401 tagaccaaat catcgacaag gtccaattct tttaagcggg ctacgatttt atttcgatct
     5461 tcttgaagga tttccttttc ttttaagtat gttcggattt ctttttccga tcgcaactga
     5521 taacttaagt aattcattgc taggtgaaaa ccgaaatcag ctccgctact cttcttgatc
     5581 tcttggatag tttcttcgtc taattcccgt ccttttaata atcgatagcg tactaataca
     5641 tcttcagata cttgtaaagc ttctccttca gataaatcta cgcgatagaa agggcctttg
     5701 cctttactga ttctcgtaac ggttaacata aacatcctcc tcgaacatat attctctatc
     5761 ataccacaga aaatccccgt aatcaggtat tatatgttag aataaaccac agaaaaaaga
     5821 aaaagggaga tacttatggc agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa
     5881 atcaagcgtt taggcataaa tggtgaagga atcggttatt tcaaacggtt gatcatattt
     5941 gttccatatg cactaccaaa agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc
     6001 tatgcagaag gagaattaat aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca
     6061 tgtcctgttt actatgaatg cggtggctgt caattacaac acttagctta tcaagctcaa
     6121 ttggatttta aaaaggatct gcttttgcaa gcattggaaa aattcaaacc agcaggcttt
     6181 cgatcttatc aacttttgcc gacgatcggg atggatgagc cttggcatta tagaaacaaa
     6241 gcacaatttc agttacgaaa aaataagcag actcaaaaaa ttgaagctgg tttatacgaa
     6301 gcaaactctc atcagttagt ccctataaca gactgcttgg ttcaagaacc tcgaacacaa
     6361 aaagtaatga atactgttgt tcaactattg aataaatttg atgcacccat ctatgatgaa
     6421 cggacaaaca gtggaatctt ccgcacgatc atggttagaa tcggtgttaa aactggggag
     6481 ttacaagttg tttttattac tcgcagtaaa aaattcccgc aaaaaaacgc aatgatctgt
     6541 gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa
     6601 acgtctctcg tgatggggga tgaaacgatc cacttatggg gaaaagaagc cattgaagaa
     6661 caaatcaatg aagtgacttt cgatctttct cctcgtgcct tctttcaact caatcctcag
     6721 cagaccgaat tgctttatgg cgaaggaatc aaagcattag atgtccagcc taatgaaacg
     6781 atcgtggatg cttattgcgg agttgggacg atcggcttaa gtgtagcaaa acaagccaaa
     6841 gaagtacggg gaatggatac cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa
     6901 agattaggat atcacaacac tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa
     6961 tggttgaatg aaggttttca gcctgatggt atcatcgtag atccaccacg tacgggtttg
     7021 gataacgcat tgatccaatc attattgaaa taccctccaa aaaaacttgt ttatatctcc
     7081 tgtaatgttt ctacacttgc cagagatctg gttaatttaa gtaaaaagtt caagattgaa
     7141 tatttacagt ctgtcgacat gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg
     7201 acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata
     7261 agaacagcca aacaaaaata gctcttcatg ttttatgaat accgtacttg tgaccgaaga
     7321 gttaacagga ggacactatt tatttaaaaa aagggttgtg acatagtttt tgtcacagcc
     7381 ctttttgatt attattggat attcacgtct atcacttgat aaaatgcatt tgtcgtatcg
     7441 ctgatattcc atactgctaa aatcacatta tatccttttc gatcctgcgg aagtgtgatc
     7501 gtttggttga ctgtttttgg tggaacagtt gctttgtcgt ctatttttgt gatcaattca
     7561 aaatcactaa atttcaaagg ttgattagga ttccaagttg gtttggtaat gtagtaatcc
     7621 caagtacttg tccgatgttg tgctgttaaa ttccaagtaa tatttaatgt tcctggatta
     7681 accatgtttt tatgccatct gttagatgtc tgttcatcta aaggctcaaa gccgctgact
     7741 cctgcactag caatttttcc atcaatgaat gtatttttgg tagcttcgat actttgcggt
     7801 tcccattgtg ctcttccgac attttgattt aagtttcctg ctgctgatgt tcctaggtat
     7861 gcacggcttc ctggagaagt aatataacca tgcgcaaaac ctggaatgct aaaaccgaac
     7921 aaccctaaac taactaaccc aataaacaat aacgtactaa tctttttcat gtagaaacct
     7981 ccttcatttg ataaccacat tgtaaacgct ttattgtatt aacacaaaca ttatgctttt
     8041 ataattttat gttgaagtaa aaaaggtgaa aaaagtaaga tgcagattat aacagaagta
     8101 gacgagaaaa gtacaaatag aaaacatgag aaccaaatca tatgatcttt ttcaaaaaca
     8161 tcattcatat aaacaaatgt tcaacttttt aataacaaca atcatcaatt tttttcttac
     8221 ttcacaaact aaatacaggt cttgctgtga gccttcatgg taaactacta ttatagaaag
     8281 gagttagtaa aatgaagatg ataaaagata ttcgtttttg ggtttgtggt ataggaattg
     8341 tcatattagg ctttttatca gggttgctta gcggaaatcc cggagaatat tactattctt
     8401 tgcaacttcc accatttgca ccacctagct ggatatttgg gccgatgtgg accttactgt
     8461 atatattgat gggtatttct ctttatttgt tacttaatca caacaacaaa aaacagcgaa
     8521 acaatcttgt cggtcttttt gtgattcagt tcatttttaa ttttatatgg tctgctctat
     8581 tttttaattt aagaaatatt ttcattgcag caatcgatat tacactttta gttattttct
     8641 tgtcagtgct tatgtatcag ttgtggctac atcatcgcct ggcaatgtgg ctcatgattc
     8701 cttattattt atgggtacta ttcgcaactc tattgaatta ttcaatttat tttttaaact
     8761 aagcgctaaa aagagacgtc ctgtaattta acacatagaa aaagtgccac ctaagttaga
     8821 ttttttgtct aatttaggtg gcactcttta ttaaccaaac tatttttttc tagtacccac
     8881 ttgttgttcc atactcatta ttctggtcat aaccataacc attttgttcc tgtccagcgt
     8941 aaggatcata actttgagta tcttgacttg gtgtatagta ctgacttgta tcttctgttt
     9001 gctgataata ggtattcgga tcttggtact gatcatatgt gttttctgaa taggaagaat
     9061 aatcggtgta cccttcacta ttcgatccat caccgtttgt cgaatcgcca tcttccgaat
     9121 catcaaagaa catcattgaa gcattctcat ttttcaaatt aggtaatgta tcgcttgttt
     9181 ttatatttaa ttgtttcttc agttcatttt ggatcgaaag aagatcattt tttcctaaaa
     9241 tttgatagta gatatcattg atcatttgtc cttcgccttg caactggtct tgtttgattg
     9301 tttcaaacgc agggatgtag ttcgtcgcta catcaagcat gtcatcccaa tctaagtccg
     9361 tcgtcacatt gttctcaact gcttttaaaa tttttctata attgcttacg ccatctaaac
     9421 ttaaaacttt attgacgatt ttcgtcacta cttcccgctg acgacgttgt cgaccgatat
     9481 cgccttctgg atcttcgtgt cgcattcgag aataggctaa acctttttct ccattcaatg
     9541 tttgttcacc tactggtaca tggacaccat ccaaagtaaa gtcaatttta ttattcactt
     9601 tgatgccacc gaccgcattg atcaaatctt ccatgccatc caaattgatc gttacataat
     9661 gatcaatagg aatatcgagc agttgctcaa ttgaatccat ggccatttcg acaccaccaa
     9721 atgcataggc atgatttaat ttatctaccg ttccataacc tacgatattc gtataaatat
     9781 ctcggtccaa actgatgatc gttgattttt tttgtttcgg attaactgtt actaccatca
     9841 tcgtatcaga acgtccttgt tccgttcttc ctaatccacc ggtatctaaa cctaatagca
     9901 aaaccgaaaa aggttctttg tcatcaatgc ttactctctc agaacgtttg gaggatattc
     9961 gattagagtt tttagaaatt ttgtttactg tttggctcgc ttctcccata aatctgattc
    10021 cgtatgctga taatccggca atcagcaaaa caaatacccc taacaaggtt aatagaactt
    10081 tttgccatcg cttcataata cacctctgtt tcacattttc taaaatattg tctcatataa
    10141 ctgttcaatc agcaaattca ctttttaatc ataagaaaac tttaaaaaac atttttgtgt
    10201 aacgattact attttttaaa atttctcaaa attcttaact atcataccat accgtgaaaa
    10261 agatcgaaag accgaataca cgcagttcgc aatcgtttta tcttttttta cgtatttttt
    10321 ccagaaaaag cgaacaacaa ggtattttcc aaataatcgt gcagtaaaat gatcgcacat
    10381 acctttccca tctttttaac agtctttgcc taccgttcag attatcattc gatttcacga
    10441 aaatttcttc ctatgactgg tccttttgtt aaacccatct tctattcgtt ttatttccag
    10501 ctcactgcat ggctcttaaa atgactcgaa ctagtgattt cattaccatc atcttttaat
    10561 ggatagatat gctcctcttc aaagtaaaac gattttcttt taaagtaata gaattgacat
    10621 ggtaatgagt atgtatacta gtggtttcac aaatggtctt ttattcaaca tccaaaaact
    10681 gaaatttctt taaaatccat tactaacgaa gaaaatgttc cttgcttgat aattagttag
    10741 gacatgtcaa gtgaacaaat gcatttactt agccaaaaaa tctaaaaact tcataaatag
    10801 acaaaaagac acgactctct aagaagtcgt gtctttattc aacaaattat tataatttag
    10861 tcacttcagc agcttgaggt ccgcgtgcac cttcaacaat cgtgaattct acttcttgac
    10921 cttcttctag gctcttgaag ccgtctcctt gaattgctga gaaatgaaca aatacgtcat
    10981 ctccgccgtc tactgtaata aacccaaatc ctttttcatt gttgaaccat ttaaccgttc
    11041 cgtgttccat aaatgtatag cctccaaaaa agttcttgca agtaaccttg caagaacatt
    11101 atacccaatt tagaaaacgc tagcaagctt tcgctctttt attttccaaa ttatttttaa
    11161 gactgatatg acggaggaaa tggttatttt gctgcttcga aacgtttgtt tacttcatcc
    11221 caattaataa cattccagaa tgctgcaata taatctggtc ggacattttt atatttcaaa
    11281 taataagcat gttcccaaac gtccaaacct agaattggtg tctttccttc catcaatgga
    11341 gaatcttgat ttgcagtaga ggtaatagct aattttccat tttccattac aagccaagcc
    11401 catccagaac caaatcgtcc agcggctgct tttttgaatt cttctttaaa agaagaaaaa
    11461 tcaccaaaag cttcattaat cgcttctttt atttctcctg taggttcgcc accagcattt
    11521 ggtgccatga tttcccagaa aaatgaatgg ttagcatgtc cgccaccatt gttacgtaca
    11581 gctgtcttga tatctgttgg aatagcgtcc atatcagata ataattcttc tattgttttt
    11641 tcgcctaatt ctgggtattt ctcaattgct gcatttaaat tcgtcacata agtattgtga
    11701 tgcttatcat gatgcagatg catcgtttct tcgtcaatat aaggttccaa tgcgtcatat
    11761 gcataaggta gatctggtaa tgtgtaagtc ataaaaaaat tcctcctcaa tgttttcttt
    11821 ataatgtcta cattacaaag agtaacaaag agaaggaaat tcttcaattg ttatgcctag
    11881 tcgtcttttt cactttgacc tacttcttct tcaaaaacct cagtttcttt aaaaataaac
    11941 aacttgctga aaatatagtt cgctaggata acaataattt gagtgatgat tttcgccaca
    12001 tactcattgc tgttcataac attgatcaac aataccattg cccccatgtc caatccgtaa
    12061 gaaaggatac ggtagaagaa gaacttaccg cattcaataa gtgcatgtgt aaaattcggt
    12121 gattttgaat ggaataccca aagtttgttc gttacaaaag caaacaatac agaacaaaac
    12181 cacgaaatag aattggataa ggccatactt aaatcaaaat attggcgtgc gagagcaaaa
    12241 ctaacaaaat ttacaatcgt agccaaacca ccaaaaaaga gatagactag tatctcccaa
    12301 tatccttttg cttctaaata tccttttaac tgtcgatact gcttcatgag ccaacctacc
    12361 ttattaaaaa ttcttattta tcatactcta tatttcaata actttcatct ttatttaaaa
    12421 aaattaattt tttttgatcc tttgcctttg tctctgcttg gatcgtcaca tgtgtaatac
    12481 catattttaa ttttaataaa gaaattatac gaggatatat cttttcaatc tcgcttagta
    12541 gctgatcttc aaaatcaata tgtccgtcga aaataattat cttttcgcta tactgccaaa
    12601 tatggacatg atgaacattc tttaccccat cgattgcttc aatttcttgt tccatttttt
    12661 catagtcaat agcaggtgca ctttgtatca aaatagcggc agcttttttg atcaccatta
    12721 tcgcttctat caatatatat atcgaaatga tcagtgtaat aactggatcg atccagtaga
    12781 tatcgaaaaa acggatcaaa attgctccga taatcacact taccgaagac agtgtatcac
    12841 ttaacaaatg caaataacta gattttatat tcaaactttc atctgcttct tgtcgtaata
    12901 ataatactga gaacaaattc gcgagcaatc caataactgc tactgttagc atcaaatgac
    12961 tatttatctt ttctggtgaa gtgaaccgct tgaatgcttc aacggttaaa aacaaggata
    13021 atatgatcaa tatgaatgag ttgatgaaag cactcaatat ttctaaacgc tggtagccat
    13081 acgtacgttt tttatttgct tcttttcggg ccattttttg tgccccgtaa gaaaaaataa
    13141 ttgccaatgt atcactaagg ttatggaaag catctgaaac aagagacaaa ctccctgaca
    13201 taatccctcc aataaattct gataaagtga tcaccgcatt caaaaccact actccaaata
    13261 gtttttttga tgatctctgt ggctgtgtag gcactattcc ttcctccatt ttatttatat
    13321 tggatagtct ttagacaaga ataacatttt tttgatattt caccaatcat tcagatgttt
    13381 ccctaaagca cttgtatttc acttgatact tcgctagaat tgcaagtgat gacactacta
    13441 gatgaggagt acttatgaca acaaaacaat taattatcag ttcctttata cgattcaaag
    13501 acctaaagga ggcgagaaat gtcccatttt ggaaagccat tgtctatctt ttagttttga
    13561 gtataattat ggctttgcca atctcttttc aagtttttca agtcttaaat aacattcaat
    13621 tggatgggca aaagattgct actcgcattc ctgattttac aatccaaaac gggcagcttc
    13681 aaaccgaaga aaaagaagga ttcatttacc aaacaaactc cattatcttc acatttgatc
    13741 cagagggaaa acgaacagaa caggacatct ctactgattt gatggggaat tttgttagtg
    13801 tcgggatgtt aaaaaacaaa cttatcatag ctcttcctaa tacaggcacg acttctgctt
    13861 tgttgaacaa taaccaatta gaacttccct acaccaatga atctttaaaa aacttaacag
    13921 gtaagcagct ccgttctttt ttaagtgaag catctatacc actttggata aaagtcctga
    13981 catttatatt ttctatttat cctagttttc tgaatctgct gatcactttg ctatttacca
    14041 acattgctgc ttttctctat gcacgctttc gactaacaaa agctactttt cttgattgtt
    14101 taaaaacatt gatctattca atttcattgc ctactataat tgcaacaatc ttgatggtct
    14161 ttcttccttc ttttgacact agcgccttca ttgcttttgc aggaatcttt atttttgctc
    14221 aagctgtcaa aggatgggca aaaatatcga tcaattaaga aataaagaca actcgttatt
    14281 tctacttcaa tagacccttt tagaggttgt gacatttttt tgtcacagcc ccttttttat
    14341 ttgaaaaata tttctttctc aaaaaacaag caaggcgctt caaagttctt cttgtatgcg
    14401 ctacacttag agtaaagaaa ggcgggaaat taactatgtc agaaagaatc atttatgcag
    14461 atcgtccatt tcgtgatgaa tttatcgaag aaatccatac tatagcgcct ggttatatct
    14521 ttaaaacaga attacaacca gaggatctac aaaatgtaga gatcagtttg ggctggaact
    14581 cagactacca agaaaaacta ttagcctctt ctaccttgaa atgggtccaa tctatctcag
    14641 ctggtgttga tcagctaccc cttgagactt tttcaaagaa acatatttta ctttcaaatg
    14701 gtagtggcat ccacagtgaa tcgattacgg agcatgtctt aggaatcatc cttgggtatt
    14761 ctcgtggtct ttttcaagca caaaaggcac agagtgacat gaaatggtta ggttcatcgg
    14821 tccattacca atctatccaa gggaaaaaaa tactaatcat tggaactggc catattggaa
    14881 aaatgattgc aaaaaaatgc aaagcattag acatgaactg ctatggaatc aatacatccg
    14941 gtcatccagc agaaggaatg gaaaagactt tttctttaaa tcaactaaag gaagttcttc
    15001 ctgaagtaga tttcgtagtc aatatcttac ctctaacgga ggaaacaact gggctctttg
    15061 atcagcatat gtttgaacac tttgatccaa aatctgtatt catcaatgtt ggccgtggtg
    15121 cttctgttaa gacacaagat ctggtgcagg ctttaaataa ccagcaactt tcctttgcag
    15181 cattagacgt ttttgaggaa gagcctcttc cacaagatca tcctctttgg aaaatggaca
    15241 atgtactgat tacttcccac attgcaggtt tgacacctga ttttcaaaag aaattaatgg
    15301 ctattttttt aaccaatctg aaaagctact ttgccaccca tgagcttcaa accaatcaag
    15361 taaaattgtc tgcaggatat taatagctga aaacaatatt ttcaaaagaa aaaagagaag
    15421 gtgacaaaaa ttgtgtcacc ctctcttttt ttattgtttt ttaaagtgat aggttggctc
    15481 ctccaggttg ggatcataat caacaagtaa atcatatcct ttaaagaacc attcatctgc
    15541 ttcctcgata aaaaaaagtg tcccatccat ttccactttc tttacaggat gttctggctg
    15601 ttcaactgac attccaatcg aaaaaccatc atgaacctct gttttgccgt agacttttcc
    15661 aaaaaaacgt attccgtaag tttctggcag atcaatttct gagtcaaacc atttttgtgc
    15721 ttttggcgtt accgttaatt tcatcccaac aacctacttt ctcttttctt tctattgatt
    15781 catcttatcg tctagaatat ccacatcgcc tgtctctcca ataaccagta gattatcatt
    15841 ttcacgaaca tattcatctg ctgcaggaga agcgataacc gtttgatcta ctcttcgtat
    15901 ggcgactact gttaagtcaa aacgttgcct gaaatttaat tcagctaatg ttttgttgaa
    15961 aaatttagga ttggagactc ggatttctgc taaagaatat ttatctgaca gctctaagta
    16021 gtctagaata ttctttgata ctaagttatg tgcaatccgt atccccatgt ctcgctcagg
    16081 gtgtacgaca cgatctgctc cgatcttctc tagtacacgg gcatggtact cattttgggc
    16141 ttttgctaaa atattcggta cgcccatttc ctttaccatc agagtaacta agatgctggc
    16201 ttggatatct tctccaatag cgacaattac atgatcgaaa tttcttatac ctaatgaacg
    16261 aagtgtcatc tcatcctgtg catttgccac aacagcatgg gtggctatat tcatatattc
    16321 attgactcga tcttcattac tgtcaatagc cagtacttct tgtccagctt cgatcagtgt
    16381 acggcagatg cttccgccaa atcgtccaag gcctacaatt gcaaaatttt gtttcaccat
    16441 tttcctcctt tagtatcttc ttttctcagt ataacaaagc ctttccaaaa acaaaagcaa
    16501 gcatcattat tgtaaaaaag ccaagaaaaa taatagttat tttctcagct tttgattatc
    16561 ctctttatca tcgtttttat ttcaccaaac catgtttaaa cgcatagata gctgcttgag
    16621 ttcggtcttc tacctctaac ttagctaata tattcgacac atgcgttttg actgttttca
    16681 gtgtgataaa caattcatcc gcgatttcct gattactttt cccttcagaa atcagcatca
    16741 aaatttcatt ttctcgattc gttaattctt catgcaatac tggatcattt cgccgactca
    16801 ttttttccat catttttgtt gtcacttcag gttctagtac tcgctcgccg cgttgcgtag
    16861 cccttatagc atcagctatt tcatgtgctg tagacgtctt caacaaatat cctgctgctc
    16921 ctgcttcaat tgcaggataa actttttcat catcaataaa acttgtgacg ataatgattt
    16981 tggcttttgg ccaatctttt aagattgctt ttgttgattc gatgccatcc atttcttcca
    17041 tgactagatc cattaaaatg acatccggtc gaagtgccat cgccttttca tagccttgac
    17101 gtccattttc agcttctcca atcacttcaa tgtcttcttg gatagataaa taagaagaga
    17161 cgcctaagcg taccatttca tggtcatcta ctaataaaac ttttatcatc tttatctatg
    17221 cctccttcat caaagggacc ttggtctcca cgctcgtacc ttgcccttta aaactaatga
    17281 tttttactgt tccaccaata ccagctactc gttctttgat attgttcaat ccataacttc
    17341 ccgttttggt ttcattcata ttaaatcctg tcccgtcgtc aatgatacgt aataaaagat
    17401 tgttatctat tttatgcaaa tagacttcta attcattagc cttagcatgt cttaatgtgt
    17461 ttgataacaa ttcttgaacg attcggaaca agtgatcctc aatggaacta gttaatttta
    17521 catcttctac ctcccatttt agcgaaatct ggattttatt ctgcaattcc ttcaataact
    17581 gctcaatgcc ttgttttaaa cttttttctt ctaaattgac tggacgcaga tgaagaagca
    17641 gcgcacgcat ctcagactgg gaagcgttga tgatctctgc caccattttc aactgcttac
    17701 ggaacatttc tggtgtctca cttttttctg cttgctccgt caatgccgac atcatcatca
    17761 tcgctgcaaa taattgctgt gaaactgaat catgaagctc acgagctaat cgatgccttt
    17821 ctaattttaa aatctcttct ttggtttgtc catctacata ttgaggacgg ctagtcataa
    17881 tctgtaattc actggatact tcgatcattt tttcttttat tttagtaatt tccatatcaa
    17941 tatctttgat gtacagatca tccgacgcgc ttgggatcgg ctgatgcaaa agtttggatt
    18001 cataattgcc agaagaaagt gcacgaagct tttcttcgat tttcccgtat tttgcctttt
    18061 gaataagtga taacaataga aatacgatca atcctacacc taatgaaatt gccatcaaat
    18121 ggaagataag cggaacgtat aataaacgcg cacgcagcaa ttctccccac caatgacttt
    18181 ggttactggc ataaaaataa gtgaaaagtg aaaatagaat aataaggaaa gccgcaatcc
    18241 ccgaataaac agctagcatt gctttggata ttcttcccat catattcgta tcacctcgac
    18301 atctccaacc aaagtattgg taatgatctt caatctgcga ggattttcat cataatcact
    18361 gctgcagatt ttgatctgtt cgttttttaa ggaaaactgc tcttcttcaa acaagacatt
    18421 tcctactaat gtcgcatgct ctaaacgaat agccactccc aggggtacta agatccgtgt
    18481 tctgcctatc ccttttctta caatcacaat attatcatct tttggcaaaa gtgtattgcc
    18541 caaatcgatg atagtatcac cagaaatgat ggcaatgttg atatcatccc attcatagac
    18601 gtcgtttcca atacgttggt taccaaataa ttgctgtctt ttacgctcat tattatgtgt
    18661 ttttaattgc tctgtttgga ccatcatgat ctgttttttt cgccaaaaag cattttttgt
    18721 caaatctatt cctgaaatct ctacaccttt cagcccgata aataaaacgg ccaatacaac
    18781 catcatccat aaagcaggac tgttgactaa gctaaaaaaa atgaccaagc tccctaagat
    18841 caattgaaaa ttttgaaatt tagtacgagg atatttgcgc atagctagat atatattgaa
    18901 aataccgaat attactaaaa gcaaaagtcc agtattgttt acgatttgcc aaacagcaaa
    18961 taacagcagc agtgcttcga ctaccacaaa aaaacgccaa gaactattca tcgctttttc
    19021 tccctcctga cacattctca tttcatcata cctatttttt tgactgttgg ctataggact
    19081 ttagatgtaa gatcgttcag tgaaacctgc aatactcaaa gagaaaagat ctagagtgcg
    19141 tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc tactttgacg
    19201 attttaacta acatatcgcc acctggtgta gcaatggtta cttcatcatt tttacgtttg
    19261 ccaatcaatg cttgcgcgat aggagaatca ttggaaatct ttccagaaaa cgggtctgct
    19321 tcagcacttc cgacaatcgt gtactcttct tcatctccat caggcaactc gatgaacgta
    19381 actgttttac cgatcgatac ctcatctgca tctacgccgt cattatcgat gatttgtgca
    19441 aaacggatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg ttcatcttta
    19501 gctgattcgt attcggagtt ttcggaaaga tctccgaaac cgcgagcgat tttgattcgt
    19561 tcaatgattt ctccgcgttt aactgttttt agttcttcta attctttttc taatttttct
    19621 ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc cttttttcga
    19681 taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt accacgcttt
    19741 tcttaaaatg taaatgtttt tctaaaaatg atcaatgact agcccctgtt tttctaagaa
    19801 ttatttacat atttttgcac tagttccata tgttcttcat acgttttagc gaaataaaca
    19861 tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg ttctaatact
    19921 gccttgattg cctcttcgct tggattgtca aaaggaccag gcccatagcc ggtattggta
    19981 tacaaattgt atggtgaatc tacagctgta tcttcatacg tcaccaattc tttatgttct
    20041 cctaatgcat ataaaacaga gatatctgac tgaagtggca tatttgcctt gattcggtta
    20101 aagaagactt gtgcaatatt tttacgatca ttttctttta cgccttcttt ttcaactaga
    20161 gaagccaaag ttaaaacttc ttgtacagtc aaatttttct gtttgatagc tgaataatag
    20221 ttttgcatga ccgtatttgt tttgttcacc atctcaatca caagatcctt aagcgaagta
    20281 ctcgtgtagt aatcataagt agctgggaat aagtagcctt ccaaacggta ttttgtgttc
    20341 tcagctttag aggcactttc caacaattct gggaattttt gatacaactc gttaaagaaa
    20401 gtctcatcat ttactaaatc taaaaattct ttcttgtctt taccagttac cttcgctacc
    20461 cgctcagcaa tctgatcgat gtcgtagcct tctggaatgg caattttccc atctgcaact
    20521 tttgttggct cagaagtgcc gccttcttgc agctgtttgc caatctcgtc aagagtcata
    20581 ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa ttttgtataa
    20641 taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc accaatctgc
    20701 ttgttagaag agccactcgg gatctccact tggactaatt ggtcatctga tttatctaaa
    20761 ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc gccaataatt
    20821 aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc ttttttacgc
    20881 agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc ttttttatct
    20941 ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca tcttcgtaac
    21001 tctgtttatt atacatgaaa tttctactgc cgtgaattat tttaccaaat attatgtcaa
    21061 aattgtaagt ggcaaatact ctttttgagg attacaatga aaaatagtta aaaatggata
    21121 gaaaggaatt aacatgtcaa aaggaaaaca tcgaaaaaaa caaaaaattt ctatttcttt
    21181 tcttgtaatt ttattattgt ctgtcagctt cttttcaaca ggcatcatta taggaggtca
    21241 attaacaaaa atacctcatt cccaaactta taaagcggag ccaacacaac ttacttccaa
    21301 tttaacgccc gatgagcaga ggcttgctag caaaattgat caattactac aaaaatcaga
    21361 ttatatcggt tcgatttatg ttcgcaaaaa taatcgcgta ctattagaaa aaggatatgg
    21421 atatgcaaat ctgcgcgtaa aagaatcgaa ttcaccttct atttactatc aaatcggttc
    21481 gattcaaaaa gcaatgactg ctttactgat actaaaacaa atccaagctg gtaagttaac
    21541 ttttgacaca aagttagcag aattttatcc tcagattcct ggaagtcagc aaatcacaat
    21601 caaaaatatg ctttatatgc gctctggtct tcatcgaact gcttctcccg acaaacctat
    21661 gtcggatagt gaaatcattc agttcgccct tcatcatttg aaacttactg attatgatac
    21721 gtatcattat gaaccattga attatacatt gcttacaggg atattgatca aactaacgaa
    21781 tcagccttat gaaactcttc taaaaaaaga aatcattcgt ccactccatc tagaacatac
    21841 agatttttat gacaatgtaa aaaattcgcc tcaacatgct gtatcttacc agatgtctgc
    21901 aactgacgat tattcaaaag cattgatcga gcctgaaacc gacatccgaa atgaattagg
    21961 tacaggaaac ataagtatgt ctgtttatga cttgaacaaa ttctttacca gtgttctttc
    22021 tggcggtatc ataccaaaag aactactttt ctcattgtgg caaaataatg gtgataaaca
    22081 tccttacagc ggcggtgttt acagtggaaa cgattatatc cttgctcagg ggaatatcaa
    22141 tcgattccat gcagtagtgg cgatgaaaaa agacactaaa gatgcaatcg tcatggaaag
    22201 taatatccaa tcggataaga acatcaaatt gccggccact gatttaagaa atcagattta
    22261 tgaactgatg gaaggtgttc acttatcaaa ttaaattttg acataatatt agttgtcatc
    22321 tctgtatagt caatttagtt agtactctat ctgaaaagta gaaataaatt aacaaatatt
    22381 atctgtcctt attatttgtt aatttatttc ttattttact taaataattt ttg
//
LOCUS       DACCQI010000045        22385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCQI010000045 DACCQI010000000
VERSION     DACCQI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.045"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            107..970
                     /locus_tag="IVO76_001318"
     CDS             107..970
                     /locus_tag="IVO76_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7429289.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IVO76_001319"
     CDS             complement(1099..2721)
                     /locus_tag="IVO76_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7429290.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IVO76_001320"
     CDS             2970..3491
                     /locus_tag="IVO76_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429291.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IVO76_001321"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IVO76_001321"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7429292.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IVO76_001322"
     CDS             5572..6390
                     /locus_tag="IVO76_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7429293.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IVO76_001323"
     CDS             complement(6493..6690)
                     /locus_tag="IVO76_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429294.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IVO76_001324"
     CDS             6859..7437
                     /locus_tag="IVO76_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7429295.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8480..8983)
                     /locus_tag="IVO76_001325"
     CDS             complement(8480..8983)
                     /locus_tag="IVO76_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7429296.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9342..10367
                     /locus_tag="IVO76_001326"
     CDS             9342..10367
                     /locus_tag="IVO76_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7429297.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10714..11592
                     /locus_tag="IVO76_001327"
     CDS             10714..11592
                     /locus_tag="IVO76_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429298.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12068..12184
                     /locus_tag="IVO76_001328"
     CDS             12068..12184
                     /locus_tag="IVO76_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7429299.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13112..13450
                     /locus_tag="IVO76_001329"
     CDS             13112..13450
                     /locus_tag="IVO76_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429300.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13544..13774
                     /locus_tag="IVO76_001330"
     CDS             13544..13774
                     /locus_tag="IVO76_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429301.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14174..14545
                     /locus_tag="IVO76_001331"
     CDS             14174..14545
                     /locus_tag="IVO76_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7429302.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14819..15511)
                     /locus_tag="IVO76_001332"
     CDS             complement(14819..15511)
                     /locus_tag="IVO76_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7429303.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15514..16299)
                     /locus_tag="IVO76_001333"
     CDS             complement(15514..16299)
                     /locus_tag="IVO76_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7429304.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16536..17936
                     /gene="lacG"
                     /locus_tag="IVO76_001334"
     CDS             16536..17936
                     /gene="lacG"
                     /locus_tag="IVO76_001334"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7429305.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17961..18278
                     /locus_tag="IVO76_001335"
     CDS             17961..18278
                     /locus_tag="IVO76_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7429306.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18291..19964
                     /locus_tag="IVO76_001336"
     CDS             18291..19964
                     /locus_tag="IVO76_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7429307.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19978..21609
                     /locus_tag="IVO76_001337"
     CDS             19978..21609
                     /locus_tag="IVO76_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7429308.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         6997 a         3739 c         4506 g         7143 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa accctttttg ttaaataagg ggaatatagc ctgtcagttt
     8341 caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa tagatttttt
     8401 taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg tctattacgg
     8461 tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat aaaaaagcta
     8521 cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct aacttatttg
     8581 tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct cctgttgtat
     8641 tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga atcaattctg
     8701 ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt ctcttgaaat
     8761 tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg gtctcacgta
     8821 ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca ttgctgtaag
     8881 aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac cataatagaa
     8941 agaataaata aatccctaat aaaaattttg atacataccg catagacatc tcctccattt
     9001 aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt ttaaggacca
     9061 acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt ttctttaaca
     9121 aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa aacgactcgg
     9181 aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact aagtttatga
     9241 aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg catttcatct
     9301 tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag acttattttc
     9361 gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta cttatcttta
     9421 ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt tcatcattag
     9481 ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg aaacgtatgt
     9541 ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg acttatgttt
     9601 tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc gtgttgcgat
     9661 tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc atgggaacga
     9721 gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt atgggtgtcg
     9781 tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct gagcagacaa
     9841 ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc attgattctg
     9901 ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg aatctgcata
     9961 atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac ttggatgcga
    10021 tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt gtgtcggcat
    10081 tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact tttctatatg
    10141 gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt cttaatccga
    10201 tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg atcgaatatc
    10261 gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg gtaatgacgg
    10321 ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag gacaaatata
    10381 tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag gtgttcaaaa
    10441 gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac tttcaaaaaa
    10501 attttatatt tattctatca aaaaatttta tatttattct atcaaaaaat tttgtttatg
    10561 atggtaactg aataaacttt cctgagatgg tcaatgcttt ccttgaaagt cttacagata
    10621 ttctttcttc atagaaagtt taagttttat agttagtgta caataaaagt atgtttatac
    10681 catatggtgt ccggataatc agtcatttaa acaatgaggt tcaaagatat gaaaagggga
    10741 aatttgatga agaaaataag caatgaaatc caaattctgg cagaaacttt aactcttccc
    10801 aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca tccttgatga ggtgtttgag
    10861 caatatagtc atgaactaat ggatcctaat aaagcaaagg cgactgcaaa gaaaatagac
    10921 aaagtctatg aagaggaacc ttttgcagca ttagctattt atctatatgc agcttctcaa
    10981 agttggaaaa aaatttatca accattgaag attccaagaa ctatctattt ggcaacaatg
    11041 aatgctttta ctagatttat tcatgaacat ttccaagtaa caggaagcta tcgctttgac
    11101 cgtggttttt ggatttggca ttacactagt ggcttgatct ttcggattgg tgaattggag
    11161 tacgaaatga catattttcc gaaagggtac acggatctaa gattagaagg aaagcagtgc
    11221 ctttctcttc atattccttc cgatgcagat ttatctcaga ataagataag agatagttac
    11281 gaaagtgcca ttcgtttttt tgaactttat tttcctgatt accattacca agtgatgtac
    11341 acagatactt ggttactatc acccaatctt actaaatggt tgaaaaaaga gtcaaaaatc
    11401 tgtctttttg cagccgatta tcgattatta tcagttgatg aacaggacga tagtggagtc
    11461 ccttggatct ttggacgagt agatgcccaa atacatgatt atccagaaag tacgagttta
    11521 cagcgacaag caaaagagca attgcttgca ggcgagcaca ttggctctgg cttaggtatt
    11581 cttacttcat aagtttagta cttcgaaata gtttaaaaga gcgtgagaca aaagtaaaga
    11641 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttcgg
    11701 aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct
    11761 tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt ctatcgaaca cacagtacgt
    11821 tcaataggta aactagctat aacaatagat attaaagtta acggacaaga gggccttttt
    11881 taggtagcta aaacgtaata atgaaaattt atttccagag ataggagtca atcagctagt
    11941 ctgatcgact gttttaacaa gttgactttc attttaactt ttgataccat ttaaaacaca
    12001 aatcgtaatt gtttcgattt gtgtttttta gcatgactac aatcaaagaa gaaagtgggt
    12061 cgtccatatg gaaaaaacag atatctcaag tgcataccgc cgcttgaaaa gcccaaatat
    12121 caaaacacga aaacgtgcgc tgaagatcat caaagatgtg aaacggaata gtgggaaaag
    12181 gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa cgtttttcgg ctattttcaa
    12241 taatcggttt ttgtgtaagg tttccgaaag aactgctgtt gattttccat ttttaacata
    12301 tgataaattg gtagaatctt tcttttcaaa ttgataccat tccctaattc cagttatata
    12361 gttaactaca gaaccacaaa gcacgtacgt ttataaaagt attatctgta gttatttttc
    12421 aattcccttt caacctctta ttttgaccag tagtattgat cactttacct tttaacacct
    12481 ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg taactacata atcaaaggtt
    12541 ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc aatacggttg caaataacca
    12601 ttatcgttta ataagcagtt acgaaagctc ccaatatctc taacgttcag ctcactccat
    12661 ttcttttctt caaaaaaatc agtgattcgt atctctttct atttgtcgaa aaagttaact
    12721 ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa agcagttact attgctagaa
    12781 taacatcaag gaataataag ttttggtttt cagataatag tcgataagtt gtgtattgga
    12841 attttagata cagtaataaa gtatttttca tgaaattttg tgagaatcac tattacaagt
    12901 atctttaata aaattaatgg ttaaggagga acttaaagta gaatgtaaat gaatagttgt
    12961 agtcagtcga ttgtgatact agactcaaag ttgcaggaat aagtttttca atttgtgatt
    13021 ggaagataaa aaatgaaaaa gaagggcttt tttttatact ttgttgattg tagtgtttat
    13081 tttccctctg tttagccaag taacattttc tatgactcaa ccagtttcaa gtgaggaaca
    13141 tgataacctt tttcgtgcta cagaagagtt taagaaatat caggaaaata ttgttccgga
    13201 gactccatta gctagtgagc caattactga tattgatgga aaccaaatag gatggtatat
    13261 acagtacgaa gttatggtgg taaataatga ctcaatagta aaaaatacag taggcttaga
    13321 gctaacaccg attgaaaagg tgagttcatt gttaacgtat atttatactg atgaaagtga
    13381 cgagactgag atcattttaa cggattactc aaaaattgct actgataaaa agtatatatt
    13441 gtggatgtag acactagtga aatactttat gatatagaaa ttgatgaaaa tttactagat
    13501 gtagcacaag aaatcgatca tcaaaagcag gaagctattc gatatgctgg tagaaaaggg
    13561 aaaagaaaat atttattacg tgaatgtagc aaaagttaga gaagatgaaa atgagtggaa
    13621 agaattcaag agtcgctact caattaactc gactccgaca tttacagttt acagagaagg
    13681 atcgatagaa aaaactgttt tttggactaa agaaagtgga atgtctttag ctgaagtaga
    13741 agagtttttg gattatgtta gtatgcaaca gtaagggagt tcttattatg aaaaattttg
    13801 acacatatta tgttattgtt ttttcgatat ctttgctgtt aatattacta actttaatgc
    13861 agtataaaga gtatccagag tattttattt taacttttgt atatttagta agtagctaca
    13921 cacttctata taaattcaga agttcttcta gagtttctaa agtttgtttc ttaattagct
    13981 tattgattct ataatatttt tgatttatat aggaaattaa aaatattata gaatcaacac
    14041 tcagagaacg ggttggtttt taatagattt aataagtact gataatgaaa tttctcctgg
    14101 ttgggaaaac gaagttggtt accctgtaga agaatttttt caagattact ttgcggagta
    14161 tgccgatagg ataatgagag attacgtaac agataaaact caagaagtca tcaatcaatc
    14221 ccagtcatta aatgaattat ctgataaggc gaaagaagaa gcagttgcca ttatttcttc
    14281 agggattagt gactatattg atatgagtgg tgaaatcaat tatggcgata aagacaaaat
    14341 tgtacgcatg ataaaagctt ttatcaaaac ttttgatgtg aaggcagatc ttaagtttaa
    14401 tgatacttac ctagtaggaa aattaattaa tatccttggg acagagaaag atacggaaac
    14461 gtttatttcc tctttgtcta tgttgtttac agataagcat ttttcaactc agcttggtgg
    14521 agaggaacta gtggaaatct tttaaccatt acgcccagca ttaataaaag tatatcaaaa
    14581 aagtactcct gatgattttt aagattggtt tgagaaataa tgtgaaaaca ctatttttaa
    14641 tgttaattat agatagagat gaacgcgtca tatggttaaa agaatgaaaa agtcaaagat
    14701 acactgctat atttcactaa ggatatagca gtgtatcttt catgtcgttt cactggcagt
    14761 tttttataaa aggaggtgtg catttcagtt gcttttcttt ggtagttagt tgaatcgttt
    14821 aagaattaac ggatgtggca gacaggtgct ctgtccaaaa accagtaaaa tctttgatgg
    14881 ttccatcttt atttagctgt gcctgttcaa atactgtttt gtttttaccg aatggatcaa
    14941 ttgcaataat tgttccgatc cccgcacgtt tggcagcaag taatcctgag taagcatctt
    15001 cgataacaag acaatcagcc ggagctaagc tcagtttgtc tgccgcttta agaaaaatat
    15061 ccggttgcgg tttcccaggg aaagagccgt cgtcatagac aatcttgtcc cagtcaaacc
    15121 agcgggctaa atcgaaaata tcaaaataaa aagcaacatt ctctttgaca gtagccgtgg
    15181 caatcgttaa tggaatctgt tggtcagtca gctggtcaag agtcgcttca agcccttttg
    15241 tcaactgaag ctcgttttca ttttgcaagc aaagcatacg ataatagctt tctttttctt
    15301 ctgacaatac ggcgatctca gaggcggtca aatgctctga aatgaaatgt ttcaaaatct
    15361 ccgtattcgt ccgaccatga atatttttta aaatatcctc gtctgataaa ccatcttttg
    15421 tatgatcatg tatcatatgc agccaagcag cttcatgcag atgagagtcc aaaaacattg
    15481 ttccgttgaa atcaaatata atccctttca tagttacgct cctatagtgg tattttcggt
    15541 tgaatcagca atttgcagat cgacatattg tgcatagtgc tcttgaacct cccgcgagat
    15601 ctggcgatcc gtaatgagca gatcgaaatc atgcaaatta taaaacacat aaaaatcatc
    15661 tcgattgaat tttttattat caacgagtag atatttctta cgggaattat ttagagcgat
    15721 ctgctgtgct tctccttctt cggtactgta agtagagatc gcttcgttgt gaacgccatt
    15781 tgaactgata aatgcttttg taaactttag tcttcttaaa ttgtcgttgg tgatcggtcc
    15841 gacaaatgcg ccggtatttt tccggaaatc accgccaatc agtagaattt ctggtccatc
    15901 tgcttgagca agaatatcaa aaactggata attatttgta atgatccgaa ttttcttgtt
    15961 cagtaaatgc gtcgccaata gttcgattgt tgtacctggt cctaagaaaa tcgtttctcc
    16021 ctcgtgaatc aaggaggctg ctgtggcagc aatttctttt ttttcttgaa tctgcagagt
    16081 ggctttttca gtgtgagaaa gttcttgatc taaagtgtag gaaagacttt gagcccctcc
    16141 atggacacgc agcagcttgc ccgatttttc taattcatct aaatctctgc gaacggtcat
    16201 atcagaaaca tctaattgat tgatgatttc attaacggta aggattcctt ttttattgac
    16261 catttctacg atagtaagca atctttcttt tttcaacatt gtcttcacct ctaatttaag
    16321 gataatctat catataaacg gacaacagtc aatctaattc gaacaaaagt aaacatttag
    16381 ggtggttttt atgtttgtta caatagattt tattgctgtt ttctggtttt tatgattatt
    16441 tgtgtttgtt tttgttgaca aatgttttgg atgcgtttac aatgcaatca gttagaaaga
    16501 gcgaaaactg attatagatt tgaggagaga tgtacatgct taaattacca gaagatttta
    16561 tttttggcgg agccacagca gcctatcagg ttgaaggagc cactaaggaa ggtggcaaag
    16621 gcgctgttgc ttgggatgac tttttagagg aacaagggcg attcagtccc gatccggcaa
    16681 gtgattttta tcatcaatat gcaaaggaca ttgaattgtg tgaacgtttt ggtgtaaatg
    16741 gtctaagatt atccattgct tggagccgca tttttcctga tggagctggg aaaccaaatc
    16801 cagaaggaat cgctttttat catcgtgtgt tcgaagaatg taagaaaaga aacgtaacgc
    16861 cgtttgtgac gttgcatcat tttgataccc ctaaacgttt gtttgaccat ggagattttt
    16921 tgaatcgaga aacaatagaa gcatttgttt cctatgccat tttttgtttt catgaattca
    16981 aagaagtcaa ggtgtggagt acctttaatg aaatctatcc agttgcaacc aatcaatatt
    17041 tgttaggtgt tttcccacca ggaatcaaat atgattttac aaagatcgtc gcctgtcttc
    17101 ataatatgat ggttgctcat gcccgtgtag tgaactattt caaagaaaac gaacttccgg
    17161 gtgaaattgg tgtagttcac tcattagaga cgaaatatgc ggcaacagac gcgccggaag
    17221 ataagcatgc tgccttttta gacgatgcat tgtcgatccg tttcttatta gatgctacct
    17281 atttaggcta ttattcaact gaaaccttga ctgctcttga tgagatatgt gaagcaaatc
    17341 aggccagcta tcattttcct gaagaagatt tcgttgaatt gaaaaaagca tccacgcgta
    17401 atgactactt agggatcaat cactaccaat gccattttgt caaagcttac gatggagaaa
    17461 atgcaatcca tcataacggt acgggtgaaa aagggacctc tgtttataaa gtgaagggaa
    17521 tcggagaacg tatttataaa gaaggaattc ctcgaactga ttgggactgg ctgatttatc
    17581 cagaaggtct atatgacttg ttgctgcgaa ttaaaagcga ttacccacat tacaacaaaa
    17641 tctatatcac tgagaacggg atggggtata aagatcagtt tgaagatggc attattatgg
    17701 atcagccgag aatcgactat ttgagagtct atttggaatc cttaagcaag gcgataacag
    17761 ccggggtaaa tgtgaagggg tattttttgt ggtcgcttat ggatcttttc tcatggacaa
    17821 atgggtataa caaaaggtat ggcttgtttt atgttgattt tgaaacccag aagcgttacc
    17881 caaaagaatc agcttactgg tacaagttgg tcagtgaaac caaaacgatt atttaataag
    17941 tgataatgga ggaagaaaag atgaataaag aggaattaca aatgcttggt tttgagatcg
    18001 ttgcatattc aggcgacgct cgcagtacat tattgaaatt attgaaagaa gtaagacaag
    18061 gaaactttga acatgttgat tcggcgttga aagaagccga cgaaaattta acccttgcac
    18121 ataattcgca aacaaaaatt ttagcggaag aagcatctgg gaaagagatg gagatgggat
    18181 tcatttttat ccatgggcag gatcatttaa tgaccacttt attgctgaga gatctgattc
    18241 aagactttat tgtactgtac agacagaatc aaggatagga gaagaacgct atggatggaa
    18301 tcattaaaca aatcgaaaaa gggaaaccat tttttgaaaa aatctcacgt aatagttatt
    18361 taggagccgt tcgtgacgga tttttaacag cgatgccagc tattttattt tcaagcattt
    18421 tcattttgat tgctgcggtt cctgagattt ttggttacca atggcctgaa tctgtctcca
    18481 catggctatg gaaagtctat aattattcga tgggtgttgt agggttactc gtttcagcaa
    18541 ccactgctcg ctgcttggct gattcgatga atcgtcgctt accgaaaaac aaagtaatca
    18601 acaccacctc tgtgatgctg gcctctgtgg taagtttcct gttgttaagc gtctctccga
    18661 ttgaaggtgg attttcaacg gaatatatgg ggacaaaggg actactggct tcctttgtcg
    18721 ctgcattttt aacagttaat ttatataagt tttgtgtgct gaaagatatt accattaaaa
    18781 tgcctaaaga agttcctgga acgatttcgc aaaccttccg agatattttt cctttctctt
    18841 tttcagtatt tgcggccgtt ttaattgatt taggcattcg acaaatcttc caaatctcat
    18901 ttgcagaagc aatcattacg gtgctgcagc cgctatttac agcagctgac gggtacttag
    18961 gaatcgctat tatctgggga gcgatggcgc tgttttggtt tgtcggtgtt catggaccgt
    19021 cgatcgtgga gccggcaatt gcggcaatta tctatgccaa tgtggaaacc aatctgcaat
    19081 tgttcaattc cggtgaacaa gcttctaaag tactgacggt gggtttaggg aattttgtag
    19141 gaactatggg aggaactggg gcaacattgg ttgttccttt tatcttccta ctctttgcta
    19201 agtctaagca gttgaaggca gttgggaaag cttcctttat tccagtaagt ttcgctgtca
    19261 acgaaccatt gctatttgcg gcacccatga ttttgaaccc ttattttttt attccatttt
    19321 tgttaacacc tgttgtaaac gtttgcctgt tcaagttttt tgtcgatgta ttgggcatga
    19381 acagctttat gtacgttttg ccttgggcaa ctccagcacc gatcggtttg gttctaggaa
    19441 cgggcatggg tatcttagct tttgttctag cagcggttct aatcgtcgtt gacttcctga
    19501 tctatctacc attttgcaaa gcatacgatg ctgtcttagt agagaaagaa gcacaaaaag
    19561 cagaggtgat cgcaaccgag gaaggtgctt ctgccgatgt acgtgaggaa gtacctgtgg
    19621 cagcgagtca agttatcagc gatgcaaata aagaaatcaa tgtgttggta ctttgtgctg
    19681 gcgcagggac aagtgcgatg ttagctaatg ctttaaaaaa aggtgcatct gaatcgaaag
    19741 caccgatcag tgcaatggcg ggtgcttatg gttctcatta cgaaatgctg caggattttg
    19801 atatggtggt cttagcaccg caagtcaatt cttactatga tgatatcaaa gaagacaccg
    19861 atcgcttagg aatcaagctg gttgcgacaa aaggagcaga atatatcgga ttaacgagag
    19921 atcctgaaaa agctgttcag tttattctga accaatttca ataaaggagg agccataatg
    19981 aaaatgggaa tttggttgcg taaagctgtt ttatttgcag ctgtgttagg ggctttgggg
    20041 gctgtttcaa aaactgttga agcatctgcc agcgaattta ttcgcggagc agatatttcg
    20101 atcctgtcag atatggaaaa atctggtgca aaatattatg aagacgatat ttcaaaagat
    20161 gccttagaga ttttaaaaaa taatggtgtc aactatgttc gactgcgttt gtggcaggat
    20221 ccttataata gtaatggaga aagttatgga gcagggacaa atgatctgga aacgacgatt
    20281 gaccttgcaa aacgagcaaa aaatctggga cttaaggtgc tgctggattt tcactacagt
    20341 gatttttggg ttgatcctgg gaaacagaat cttcctaaag catggcaagg tctgaccttt
    20401 gaagaaatga atacggcatt gtacgactat acaaagaacg ttctgtcgga aatgaagcag
    20461 ctggacgttt atcctgatat ggttcagatc ggcaatgaac tgaatagcgg aatgctctgg
    20521 ccttatggca agtcttgggg agaaggcggg ggagaatttg atcgattggc tgcttttttg
    20581 aagtctggta tccaagctgt tcgagacact caacctaaaa caactccagt aatgctccac
    20641 ttagctgatg gtggcgatac aggagcattt acttggtggt ttgatgaaat cacgagtcga
    20701 ggtgtgtcat ttgatttaat tggagtgtcg tattatccgt attggcacgg ttcaatgaat
    20761 gaacttcaaa ccaatatgaa tgcgatcagc caacggtatg gtaaagaagt gatcgtagtt
    20821 gaaacagctt atgggcatac aacagcgaat gccgatacta tgccaaatgc ttttggtgaa
    20881 gccgaagcag ctgcgggggg atatgaacca acaccgacag gacaagcaga gtatttgctg
    20941 gatcttgccg atcggattca ggcagtaccg aataatcgcg gggcgggctt cttctattgg
    21001 gaacctcttt ggtacaacgg aaatgtgagt tgggcaacac aagcaggtat gaattatctc
    21061 agtgttcaat ccactatggg aaacgaatgg gacaaccaag cgatgtttga ttttgctgga
    21121 aacgccttgc caagcttgcg tgcgtttaaa caagcagggg cgcaaataaa tctagttaaa
    21181 aatgcaagtt tcgaaagcga tggaccaacg accagtcctt ccaattggca aacctggttt
    21241 caacaaggaa ccgctaaggg tacgatcaaa acggaagcgg gcactttttt tggggactac
    21301 aaactaactt tttggcagga tcaagcatac gaggcatcag cttatcaaaa tgttagtggc
    21361 ataagcaaag gaacttacac attgagtgcc tatgtaatgt ctgggggaga gtttgatact
    21421 aatcagttat atatcaaaaa ttatggtgga gcggaactaa atactgcggt tgttaaaaca
    21481 agtgaatgga caaaaattac aatcaaagac attccaatta caacaggtgt ttgtgaaatt
    21541 ggggtttata gcaaggcttc aggaaatgct tggacgaacg ttgattttgt ttcattggtg
    21601 aaacaataga ctgcggatcg tttcgattca agatcggagg ttcccttcag ccacatcgtt
    21661 agcgtagaag gcaggaatcg aacctgcacc tcgcctttgc caaaaataac cagaaacgat
    21721 ttccagtcat ttttaccaaa acgagtaaag caccagactt catctacact ttgtattatg
    21781 ctaaagagag ttcaacgatt tcgtaagaag caatgaaaaa attgcaaaaa gttgtgcaga
    21841 taacataaag tttgtttaag aaagggtaac cttagaattc cgtcgtgaat atgatttgag
    21901 cacttagatg gatagaaaaa agttctcgga aaacagttcg tttttcgaga acttcttttt
    21961 atgataagaa tgaaacaaat taacttatat tatagtctct tttggtaggg aaccatctta
    22021 tttttaatga aaatcaagca ttttagctat tgaagaatgt tgatttatag ggattccccc
    22081 tccatattat gttggctctt tattcccact ctgttttcaa cgtaaagtgt ataaaacaga
    22141 atgcgatact atcaagggag ttaaaacaat atacagaaca gtaaaacact ataaataaaa
    22201 ataaaagtgc aataaaagtg caatttttgc acctaatcat actctgttta tatatgaaaa
    22261 ctagattctg aatccaatat ttggttaacg agaggcgttc gaatttgaac gtcttttata
    22321 tattactttc aaataaatat gaggtagaat actatctatt ttctttttaa cccaattgtt
    22381 ataat
//
LOCUS       DACCQI010000046        22288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCQI010000046 DACCQI010000000
VERSION     DACCQI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.046"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(34..234)
                     /locus_tag="IVO76_001338"
     CDS             complement(34..234)
                     /locus_tag="IVO76_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7429309.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(539..1771)
                     /locus_tag="IVO76_001339"
     CDS             complement(539..1771)
                     /locus_tag="IVO76_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7429310.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2025..2594
                     /locus_tag="IVO76_001340"
     CDS             2025..2594
                     /locus_tag="IVO76_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7429311.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2772..3212)
                     /locus_tag="IVO76_001341"
     CDS             complement(2772..3212)
                     /locus_tag="IVO76_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7429312.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3370..4134
                     /gene="map"
                     /locus_tag="IVO76_001342"
     CDS             3370..4134
                     /gene="map"
                     /locus_tag="IVO76_001342"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7429313.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4166..5089
                     /locus_tag="IVO76_001343"
     CDS             4166..5089
                     /locus_tag="IVO76_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7429314.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5165..6304
                     /locus_tag="IVO76_001344"
     CDS             5165..6304
                     /locus_tag="IVO76_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7429315.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQLILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6297..7097
                     /locus_tag="IVO76_001345"
     CDS             6297..7097
                     /locus_tag="IVO76_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7429316.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7097..7924
                     /locus_tag="IVO76_001346"
     CDS             7097..7924
                     /locus_tag="IVO76_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7429317.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7902..8636
                     /locus_tag="IVO76_001347"
     CDS             7902..8636
                     /locus_tag="IVO76_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7429318.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8736..9602
                     /gene="rfbA"
                     /locus_tag="IVO76_001348"
     CDS             8736..9602
                     /gene="rfbA"
                     /locus_tag="IVO76_001348"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7429319.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9616..10188
                     /gene="rfbC"
                     /locus_tag="IVO76_001349"
     CDS             9616..10188
                     /gene="rfbC"
                     /locus_tag="IVO76_001349"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7429320.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10210..11238
                     /gene="rfbB"
                     /locus_tag="IVO76_001350"
     CDS             10210..11238
                     /gene="rfbB"
                     /locus_tag="IVO76_001350"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7429321.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11336..12187
                     /gene="rfbD"
                     /locus_tag="IVO76_001351"
     CDS             11336..12187
                     /gene="rfbD"
                     /locus_tag="IVO76_001351"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7429322.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12222..14255
                     /locus_tag="IVO76_001352"
     CDS             12222..14255
                     /locus_tag="IVO76_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429323.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14299..15579
                     /locus_tag="IVO76_001353"
     CDS             14299..15579
                     /locus_tag="IVO76_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7429324.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15789..16595
                     /locus_tag="IVO76_001354"
     CDS             15789..16595
                     /locus_tag="IVO76_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429325.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16607..17827
                     /locus_tag="IVO76_001355"
     CDS             16607..17827
                     /locus_tag="IVO76_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429326.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17817..19403
                     /locus_tag="IVO76_001356"
     CDS             17817..19403
                     /locus_tag="IVO76_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7429327.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19442..21580
                     /locus_tag="IVO76_001357"
     CDS             19442..21580
                     /locus_tag="IVO76_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7429328.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21648..>22288)
                     /locus_tag="IVO76_001358"
     CDS             complement(21648..>22288)
                     /locus_tag="IVO76_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP7429329.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         6997 a         3774 c         4635 g         6882 t
ORIGIN      
        1 aaataacccc tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt
       61 ttctaaactg ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag
      121 taaaatagat gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga
      181 aaaattgatt actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca
      241 atagattcga attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata
      301 tcgttcttaa ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg
      361 gagatgactt tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt
      421 tcaaagtacc atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat
      481 agcttagatc ctttattcta tgctttatat tattttttgc atgtgtgcga aagtatactc
      541 aagcccaatc gccatttcgg aaaatcggta ctgtagagcc atcttcacgg attccgtcga
      601 tatccatctt gtcagatcct accatgaaat ctacgtgggt ttgacttcta ttcaatccag
      661 cttctgctaa ttcttcatcg gacatttctg taccgccttt cacactgaag gcataagcag
      721 aacctagtgc taagtgattc gatgcgtttt catcaaataa tgtattgaaa aagatgatgc
      781 cagattgaga aataggtgaa gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac
      841 cttcatctgt atcaagcagt tttgctaaga catcttctcc ttgttcggca gaaaaatcaa
      901 caacttttcc atctttaaaa gtaaatttca tacccgagat gatggtgcca gcataactca
      961 gtggctttgt acttgaaatg taaccatcta ctcgatgact gtcaggtgct gtgaacactt
     1021 cttccgtcgg catatttgcc atgaattttt ctccacgagc attatagctt ccagcgcctt
     1081 cccaaagatg gtttttaggc aatccgataa tgatatctgt accgggagca gtatagtgaa
     1141 gggcagaaaa ttgttctcga ttcaattctt ctgctttttt tgctaatttt tcatcatgct
     1201 tcttccatgc taagacaggg tcttcttcat agacacgagt cgttttgaaa atctgatccc
     1261 ataatgcctc aacttgtttt tcttctggca gttctgggaa gacttttgct gcccattgtt
     1321 taccagccgc agccacgaca gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat
     1381 tcattagtgc tttgccattc gctgattgat aagacgctac tcgatggcta tctacaccag
     1441 caaatgcatc gggatcggca gaaacaacgc tgatcctgct tgctcctttt tcaagccatt
     1501 catccgcttg atcgatttta gattgtggta cattctcaat acgatccgtt gcagcatgta
     1561 aaagaaattc tctttggatc tggtcgtcag tccattgaac gataacctca gcggctccta
     1621 gtttataagc ttcttgtgtg atcaatcgag caaggggtgc ctgttccacg ctgatctgta
     1681 aaacaactgt gtgtcctttt tctgtggcta cgccagtttc tgcaattaag cgtgcatatt
     1741 tttttagaag ttcattaaaa tcagataaca taataaaaat tcctcctcac cattttttct
     1801 ggtaactatt agcatgatac catttaatta tgattcaaac aatgaggctt taaataaaaa
     1861 aaggaggtta tttttttaag gttaagtttc atgatatatg ttactacttg ctatttcagc
     1921 ttgtgaagaa aatcaaaaac taattttgca tatcaaaaaa acatgttaca atgaaattaa
     1981 tcaatggtgg aagcgttata aaaaaattaa ggagattaaa aaatatggca agaaaaaaga
     2041 cgattactag agaacagatt ttggcagctg cttatgaagt agctgccacc gatggattta
     2101 cacgatttac tgcgagaaac atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc
     2161 ttgagtttaa aaatatggat gacttgagac aagctctttt tgagcagatc tatgatcatt
     2221 tacaatatga agtgttcact gttgagcata cagggaatac gattatcgat cttgctttta
     2281 attatatcca ttttgcaaga agagagaaga agttgtatcg ttcactgtac ttagaagaat
     2341 atggtggagg aaaagagatg cagaatttct cttatactta ctttgtgaat acagtgaaac
     2401 gcgatccagc ttatgtgaat ctttcagaag agcagatcga atctttgtat aatgggacat
     2461 ggatcatagc tactgggatc gcagctttga tgtcttcaag catcatccat ccaactgatg
     2521 aacagattga aaagatgatc caagacagta tagatgctat cttgaaattc aaagaaccaa
     2581 tcgaaatcga ttgaatatac tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc
     2641 aaaataagga aaagttcgtt tttcttacag cgtatgcatt tttaactccc gaagaactct
     2701 tccatgaatg caatgaataa aagagccata cagattgaat aaatctggat ggctctttta
     2761 ttgcaagtat attaaatttt tgcgactagt tctttagcga aagcttctaa attttcgata
     2821 tcttcttctt cagcattcag atccacttta acattttcgg ctccttttga tgcgcctgtt
     2881 tttgcaaaag cagcatcgaa atcatccacc gatttgcaga attcatcgta aaaagtatca
     2941 ccagatccgc agacaccgta aatttttccc aatagatcca attcttgcaa gtcttcataa
     3001 aagtctacga tctcatcagg cagatcgcca tcatcgtatg tataagtagc tactacgcaa
     3061 atatctgcct cctcgaattc atctgcatct acttgggtac attcgttgat ttcgacttcg
     3121 atatccagat tttcaaatgc ttctgcaaca atatcggcga tttcttcagt gtttcctgtc
     3181 atgctagcat atacgatttt tgctaaagcc atgatgtgcc tcctcgttta taattattta
     3241 acaattatga attataccaa aacatcattg aaaagaaaat aaaacctaaa gacacctggg
     3301 gaatgtcatt tggctaaatg tatgccgcta tgttaaaatg agagggattt gtaaaaagga
     3361 gacgaaaata tgattacttt aaaatcagca cgtgaaattg aaatgatggc agaatctgga
     3421 gctttattag cagatgtaca taaaaatctg cgtgatttta taaaaccagg aatcacaagt
     3481 tgggatattg aggtatttgt cagaaactat atcgagagtc atggcggaat tgctgcgcaa
     3541 attggctttg aagggtatga atatgcgact tgtatcagta taaatgatga aatttgtcat
     3601 ggattccctc gtaaaaagcc tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc
     3661 gatttgaaag gcggcatttc tgattcttgt tgggcctatg tagttggaga atctacacct
     3721 gagatcgacc gattgatgga agtaacgaag aaagctttgt acatcggtat tgagcaagca
     3781 caagtaggga atcggattgg tgacatcggt catgctatcc aaacttatgt agaaggcgaa
     3841 aatctagcga tcgttcgtga ttttattggt catggcgttg gtcctacgat tcatgaagaa
     3901 ccagcagttc ctcattacgg agaagctgga aaagggttgc ggttgaaaga aggcatggtt
     3961 atcacgatcg aacctatggt taataccgga acttggaaaa tgaaaatgga tcctaacggc
     4021 tggactgcgt atacaagaga cggcggactt tcttgccaat atgaacatac attggctatt
     4081 actaaagatg gtccaagaat tttgacatca caaggagaag aaggcactta ttgatctatc
     4141 cagtcaatag gtacgggaga ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa
     4201 tctgatgcgc tttattgaga cgactcaaaa gcgcatcact gattcagaaa tgagtacgac
     4261 ctctgtcgtc gttgcttatt atttactctt gtctttattt ccattgatta ttgcttttgg
     4321 taatatttta ccgtttctcc acatcgatca agagactgta ctgacctatt tacgagaagt
     4381 gattcctgag accgtttacc agtttatcgg tcctgcaatc aaagaccttt tgacacaaag
     4441 ttcaggaggc ttgttatccg tttctgcatt agcggcttta tggtctgcaa gtcaaagtat
     4501 caatgctttg caaattgcga tgaataaagc gtatggcgta gaaaatagaa aaaatttttt
     4561 gatcgtccgt ttcttctcac taattgtcat tgtcttgttc atgattgcta ttagtggggt
     4621 aactatcgtt cttgggttgg gacagctgat tttggatgct attcagccaa tcgtacaaat
     4681 acccgtgaac tttatcgatc agttccaaac tttgaagtgg ccaatcacat tggtagcttt
     4741 atttgtcatc atgttcttga tttatttaat tgtccctaat gctcagttga agttgaagac
     4801 gatcattccg ggcacgatct tcgctactgt cggctggatg ctgttatcac aagtgtttag
     4861 tatctatgct aggtattttg caacgaggat cagcggttat cagatcatag gaagttttat
     4921 tgtactcatg ctttggctta attttgctgc tacgatcatc attttgggcg gaatcatcaa
     4981 tgcagttgtt caagaatacg tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg
     5041 aaaatggttc aataaattga aaactaaatt ttctcataaa aaaaagtaga aacaagagtt
     5101 cttattgtaa atttatatca gtaatttata ataattatga ctcttaatta aagtaggtgc
     5161 ccatatgctt gtttctttta gtatggttat aagatttttt ctgacaattt tattatcact
     5221 gatattaaca cctgtattca aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa
     5281 tgaaagaaga ataaataaag ttccaatgcc ttcagcgggc ggactgccta tctttatttc
     5341 atttgttata tcgactttat tcttattccg taaaattatc cctcaatcgt atattgtacc
     5401 gatcttgatt gcttctttag taatcatagt cacaggagtg ctcgatgata aatatgagct
     5461 gacacctaaa caaaaaagta tagggatctt gattgcttct cttattattt attttgtagc
     5521 agatatacgt attgattcct tcacgcttcc ttttattgga tatatccaac taggctggct
     5581 tagttttccc gtaacgattt tttggatctt cggtatcacg aatgcggtca atctgattga
     5641 cggtttggat gggctggcgg cagggatttc attgatcggt ttaattacga taggaatcat
     5701 tggatatttt ttcctccatg cttctacagt gtatattcca gttgttattt tctgtcttgt
     5761 agcaagtatt atcggttttt ttccttacaa tttttatccg gcaaagattt atttaggcga
     5821 tacaggagct ctattcctag ggtttatgat ggccgtcctt tcactacaag ggctgaagaa
     5881 cgtgactttt gtttcatcga tttctttgct gatcgtcatg ggagtgccgg taacagacac
     5941 gttttttgct atcattagga gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa
     6001 acatcttcat caccgactaa tcagtttagg ctttactcat aaaggtgctg tcctgacgat
     6061 ttatgcgatg gctttgatgt tttctttcac agctatggtc atgaattata ctggaagaat
     6121 cggaacgatc atattgatta ttgccatgtt gtttgctgca attttattat ttgaattgat
     6181 tggtctgatc aatgagaaac atcagttaat tttaaaaaca ttgcgatttt tagggaataa
     6241 agagtaccgt acgcaagtaa aacagaaata tttgaagaaa tggaaccgtc atatagatga
     6301 ataacaaatt aaaccagccg atccttatta gtattgtgac ggcaaacagt gataaaattt
     6361 ttcggacact agataatttt atttcaactg taaaagatga aacagcagta aaaatccgaa
     6421 tttttgacaa caattcactt cccgagtaca aaaaaagact aacagagtat aggaaatatc
     6481 cctacatcga tatctatttt catgatgaaa attccggatt tggttatgga cataatcaca
     6541 atctgatagc tagcgattct cgctatggca ttatttgtaa cccagatatt ttagtggatg
     6601 aagagacagt tacagcattg gtcttgttat tgaaagaaca tccggaatgt gcgatgttag
     6661 cacctaagat tcttaatgaa gatggaacaa ctcagcacct catccgtgag cagctggctg
     6721 tttttgatta cgttttgcgc tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac
     6781 gactggctgt gtttgaatgc cgaggattgc ctactgatgc taatagttat gtacggatga
     6841 tctcaggtag ttttatggta gctgatattg ataaatttaa agagataaag gggtttgatg
     6901 agcgtttctt catgtacttt gaagacaacg acttgtgttt gagttttgaa aaagcaggtt
     6961 acaagttatt gtacacaccc ttttatgcag ttatccattt ttacgggaag ggtgcgcatc
     7021 gaaatgcaaa actgtttcgc gtgtttttga cttctatgat gaaatttttc aataagtggg
     7081 ggtggcgttt tttctgatga aaccagaaat attagtagtc gttgtgcttt atcagcaact
     7141 attcagtcaa tctccagctt atggaccatt aagcaaagca cttagcgaaa agcaaattca
     7201 gcttattatt tacgataata gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa
     7261 tatcgtttat catcatgatc cgcaaaatcc tggtttagcg gtggcttata attttgcgtt
     7321 gtctcaagca gatgaaggaa cacgttgtct cgtcactctg gatcaggata cgaggctagt
     7381 cgacgattat tttgaactat tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt
     7441 tccgatgatt ttttctggga gccgacaagt ttctccccta tattcagatc gttatatcaa
     7501 tcgtacagct caagcagtag aagttgaagg cccaacagct gaacgaatca tggcaatcaa
     7561 ttcaggtgtt gcctggtctg tcaaatttct aaaaaagatt ggcgggttta atcctgcttt
     7621 ttctttagat tttttggatc attggctgtt ttggaaagtg gatcagttgg aaaaaactgt
     7681 ggagatcctt cctgtacgaa tggaacatga tctttcggtt ttagactatg aaaaagtttc
     7741 aagtaatcga taccgttcta ttttacaagc agaaaatcga ttttatcatg aatatgatca
     7801 aaggcattta tccagtcacc gacgacaatt attgttaaga acagctaaac agttcttgac
     7861 cgtcaaaaac cggcatatct ggcggatgac tttgcgctca tatgttaaga attggaaggt
     7921 gtgaattcgt gcgttcggtt tgtatagcta cctttaatgg aggcagatat ttaagagagc
     7981 agttagatag tattttatct cagctttcag cagaagatga agtagtcatt tctgatgatg
     8041 ggtcagtaga tgatacatgg gatatattaa ctgaatacgc agaaaaagat aagcgaatcc
     8101 agcttttttc ggggcctcgt caaggattga tcgctaattt tggttatgcg atccagcaga
     8161 cgaaaggaga tctaatcttt ttggcagacc aagatgatgt ttggttggag acaaaagtga
     8221 aaaagatcac tgactatttt gagcagaatc cggatcagct agtagtagtt tctgatttaa
     8281 tcattgtgga cagcgagctg caagagatcc atgattcgta tttttcgttt cgaaattcca
     8341 aagaaggatg gcttaataat gtgataagaa gtcactatat tggtgctggt atggctttcc
     8401 ggtcttcttt aaaacccgat attttgccga tcccgtcaga agtgccgatg catgatatgt
     8461 ggattggttt gctgggagga aagaagactg ggttcctaag agaaccgtta actctgtacc
     8521 gaagacatga tttaaacgcg agtgagattg aaacacattc taaattacgg caaaaaattt
     8581 cttggcgtgt tcatctgatt acggcgttga tcaaacgaaa atggtttaaa cgttgataaa
     8641 agatgtttaa atccttaata tttccatgac aaatataaca attgatgaga taataacaaa
     8701 gtgaaatttg aaatcaaaaa ggagagcaga tatccatgaa gggaatcatt cttgctgggg
     8761 gcagcggaac acgtctgtac cctttaacaa aggcaacatc gaaacaattg atgccgattt
     8821 atgacaaacc aatgatttat tatccaatgt ctactctgat gttggcggga atcaaagaaa
     8881 ttctgattat ttcaacgcca caagatacac cacgtttcaa ggaattattt ggaaacggaa
     8941 atgatttagg actgcagatc gaatatgcag tacaagaaag tccagatgga ttggcacaag
     9001 cgtttatcat tggagaagaa tttattggag atgatagtgt ctgcttggta ttaggggaca
     9061 atatttacta tggtggcggt ctgtctaaaa tgttgcaacg agcagcttca aaagatacag
     9121 gcgcgacagt atttggctat catgtcaatg atccagaacg ctttggtgta gtagaatttg
     9181 atgagaatat gcaagctttg tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg
     9241 ctgtaactgg cctttatttt tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac
     9301 cttcagaacg tggagaacta gagattacgg atgtcaataa agcatatttg gataaaggaa
     9361 aactatccgt agaacttatg ggacgcggat ttgcttggct tgatacagga acacatgaat
     9421 cattgcttga agcatctacc tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg
     9481 cttgtttgga agaaattgct taccgtatgg gctacatcga taaggaacaa ttgctggccc
     9541 ttgcacagcc attgaaaaaa aatcaatacg gtcaatattt attgaatttg gccgctgaat
     9601 aaggagtcct tacagatgaa agtaacagat acaaaattaa cagatgtcaa aatcgtagaa
     9661 atggacgtct ttggcgatca tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt
     9721 gcagaacatg gattgaaata tgactttgtt caagacaacc attcactatc agctgaagct
     9781 ggtgtccttc gggggcttca tttccaaaaa ggagaagctg cacagacaaa attgattcgg
     9841 gtagtcactg gagcagtttt agatgtagta gtggatatcc gcaaaggtag cccgacttat
     9901 ggtcaatggg acggatttat cttatctgaa cataatcatc gacagttgct tgtacctaaa
     9961 ggatatgccc atggattcgt tactttgaca ccaaatgtga acttccttta taagtgtgat
    10021 aattactata atgcagaagc agatgggggc atcgcatttg atgatccaga tttagcgatt
    10081 gattggccaa ttgatccatc aagagcgatt atgtcggaaa aagatcaccg tcatccgaca
    10141 ttaaaagaat ttgaagcaga aaatccattt gtttacggag aaatctaaga attaaggaga
    10201 aattttctta tgaaaaacat cattgttacc ggaggtgccg gattcatcgg ctctaatttc
    10261 gttcattacg tggtaaacaa tcatccagaa gtgcatgtca ctgttttgga taaactgact
    10321 tatgcaggaa acaaagagaa cttggccgga cttcctagcg atcgcgtaga attggtcgta
    10381 ggagatgttg cagatgcaga attagtggac cgtctagtaa aagaagcgga cgcagttgtt
    10441 cattacgcgg cagaatccca taacgataat tcattaaaag atcctttccc atttgtacaa
    10501 acgaatatca ttggtacgta tacactttta gaagcttgcc gcaagtatga tgtacgctat
    10561 catcacgtat ctactgacga ggtttacggc gatcttccat tacgtgaaga cctgccagga
    10621 catggggaag gcgaaggaga aaaattcact gcggaaacac catataatcc ttcaagtcca
    10681 tattcttcta caaaagctgg ttcagatctg ttagtaaaag catgggtgcg ttcttttggc
    10741 ttacgtgcaa ccatttcaaa ctgttcaaac aactatggtc cttatcagca tattgaaaaa
    10801 tttattcctc gccaagtaac gagtatctta agcggtattc gtccaaaatt atatggcgaa
    10861 ggaaagaatg ttcgtgactg gatccatacg aatgatcatt cttcagcagt gtggctgatt
    10921 ttgacgaaag gcagaattgg cgaaacctat ctgatcggtg cagacggtga agaagataat
    10981 aagaccgtga tggaattgat cttagaaatg atgggccaac caaaagacgc atacgaccat
    11041 gtaaacgacc gcgcagggca cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa
    11101 gagctaggtt ggcagcctga gtttacgaac ttccgtgatg ggttggctga cacgatcaaa
    11161 tggtacgaag aacatgaaga ctggtggaaa aaagaaaaag aggccgttga agcagcatat
    11221 gccaaaaatg gacaataata cagaggaaat ttcactgtat taatggaaaa acacatgtga
    11281 ggcaggcgct cgcctgtctc ttttttaaaa taaaaatcaa ggagcggata accatatgtt
    11341 tttattaact ggaggaaatg gacaattagg tacagaattg cgccatttat tagatgaaaa
    11401 aaatctgcca tatgtttcaa cagatgcaaa agacttggat atcacagatg ctgagaaaac
    11461 aatggcttat attacagaat taaaacccga tgttattttc cattgcgcag cttacacagc
    11521 agtagataaa gcagaagaag aagcaaaaga attagatgag aaaatcaatg tcgatggcac
    11581 aagaaatgta gcacttgcag caaaagctgt gaatgcacaa ttcgtctata tcagtacaga
    11641 ctatgttttc gatggaaaaa acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc
    11701 actaaatgaa tatggtcgta cgaaattatt aggcgaaaaa gcagtagaag agattttaga
    11761 agactattat atcatacgaa cttcttgggt tttcgggatc tatggacaca atttcgtctt
    11821 cacgatgcaa cgcctagcgg aaacccgcga caaactgact gtagtaaatg accagtttgg
    11881 gcgaccaact tggacacgta ctttggcaga atttatggta tatgtcatcg aagaaaaagc
    11941 accatttggt atttatcatt tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa
    12001 agaaatattg aaagatacag atgtagaagt tgcacctgtt acgtcagaag aatttccgca
    12061 aaaagcaaca cgtccgcagt attcagtcat gagcctgaaa aagacagaag cgttaggttt
    12121 tgtcatacca acttggcaag aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa
    12181 taagtaattt cctttttatc aaatagaatt tggagcatga tatggaaaaa caaacaagaa
    12241 gtgcacgacg aaaagaaaag ccaaaagcga atcaggcaga ttcgaatttg gtgcttgacc
    12301 tgtttctaaa actccgctat cttattggcg ttttagtcat cgtggtcatc gttactttta
    12361 atttaaacgg aagttcactt ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga
    12421 aaaaaactga tgtgattttt ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc
    12481 agacaccttt ttacctctca caagctgaag aggacttttc gttggtgaac gaggattatt
    12541 cattaaacgg acaaaacatg atcattgcgt ataattctcc tgtaaaggat attacggtta
    12601 tcggaaaacc attcaactgg ggattctttt tcctcggaag agatcgagga ctttcctttt
    12661 attgggcgat gaaattagtc gtcatggtct tgcttggatt cgaagtactg atgatattaa
    12721 cgaagaggaa aaaagctcta tcgctaatcg gtgcatttgc gctgacattt tctccagcag
    12781 tccagtggtg gttcatgcaa catgtgggcg atttgatttt ctttactttg ggactgatga
    12841 ttgcttttta tcattatttc tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc
    12901 ttttggtggt tattttcggt attgggttta ttttagtgat ttacccagcc catcaagtga
    12961 tgctggcgta cttgctgatt ttttattttg ttggcctgct tatttactat agcaagaaag
    13021 tttcatggga tctgttggat gtagtgttga ttggaggggc agttttattt attggcggag
    13081 tcatggttca tttctggcta acgtcaaaag acgcattgtt agcttctttg aataccttat
    13141 atccaggaaa acgggtaagt actggaggaa agtggacgat tggcgaattt ttttctttcc
    13201 ttacaaattg gaaaattcct tttgaagata tcaactattc gaataactct gaagtggccc
    13261 tgttctatca cttttttcca actgtattcc tagcaagtcc atttgtgtta tttggcaaaa
    13321 aagatagcga acaaaagtta ctgggacgta ttttgatgat tttttgtctg ttttctatct
    13381 tttggattac cgttgggttg ccaaaatggc tagcagaaat cactctgctg tcttatgttc
    13441 ctccattaag agccatcctt acttttagtt ttgcggcttg tttattatcg atctggttta
    13501 tcgcttatat ttggagagaa gatgttattc ctatttggta taaattcatt ttgttggcag
    13561 taaatgcatg gtcttatttc tatgcgatta cgcattcgaa aatggataat tatttttcgg
    13621 attttgaaac catcgcagtt gctgtgttgg gaacagtgat tttagctttt gcattatttg
    13681 gctggagacg cttgttttac ctcgcttttg tcgccttagt tatcgtatct ggctttttcg
    13741 tcaatccagt cgtagaaggt acaggagcga tttttgaaaa aacactggca aaagaaatcc
    13801 aaaaaatcga tcaacaagat cctggtaaag tttggttgtc tgaagatgaa ttatataact
    13861 tcaccccaac attgggagtg aaatccttca atacagtacg cttttatcca gatatagctg
    13921 catggaagaa aatcgatccg aaagaagaat acgagaagta ttataatcgg tatgctcata
    13981 caagagcttt tatcgctaag gaagatacaa agttcatttt agatcgtcca gatatgtttt
    14041 cggtcacgtt gaattttgaa gacgcagaaa aattagggat cgactatgtg atcagtaaac
    14101 gaccattaac tgattacaac caattatatg aagcgcagtt cacgcagcta tatggaccgg
    14161 ataaagacgg ttatatgatt tttaaagtag aatatccgga ttatccagat gtaccacaaa
    14221 cacaaactca ggaaaatcag ctagtcgtac agtaataggc tagctgagaa taatcctgaa
    14281 atattagtag aaaggagagt gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa
    14341 atgagtgtcg gtttcttgct tttgttgctg acgctagtct tttttgtttg gacagcaggg
    14401 gtaggttttc cagcaacttt gtttatatat aacaatagtt cattacttat cgcttttagt
    14461 gtggctattt tgctcatttt aaatgtttat aaagcaaaag gcgtcgattt tctttatcta
    14521 ggtgttgcgc ttgccctttt cattttcttt tcttactttc gggaattaag aaatgagtct
    14581 ttatttggag acttactgtt tcctttgatt attttatttt gtttagtaat taaatggatc
    14641 gagtggacaa aatgggatcg cggtatttat ctgattgtct ttacggtctt cctcggaatc
    14701 acgttatatc gtgtatttac agagatccaa gtaccagaag gacaaagcat ctgggctcca
    14761 ggcaattcgc tttctgatat ttggatcaat acgaatacaa tcggaagttc tttgatgact
    14821 cttgctttat tgatcagtgg atttgccagt tcttttgaac gatggtatgt tcgaatcatc
    14881 ggtgggatag cagtcgttgc agccttttta ggtatttggg tctgccaatc tagaggtgca
    14941 ttagtcgcgt tgattgtttt tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc
    15001 attcgtgcgc catttattgc ctacacggtt acgattcttt tagctttgcc gatttcttat
    15061 ctggcggctg tctctgaaaa agtcaatttg tttaccggtc gagaagatat ttggcataaa
    15121 ttctatcaga cgctaggaga aaaatctgaa caagttttac taggaatgaa aacatttatc
    15181 ttccagcgag gaaaccaatt tttaggaaac cacaatagtt ataattctat tttgaatatc
    15241 tatggattga taggatttgg gattgctgct ttgctgctta tcctttttat tggtcgctta
    15301 acgttgaaag cagatttatc caacgggcag atgactttta tctgggcctt ttttgcggtg
    15361 atgatgcagt catttatgga ggatacattg acttcatacc catgggttcc gattatttat
    15421 atcctgttgg ctatggccgt tcatcgatat gatgaaccag aagttacgga ggaattctat
    15481 gttatggaag aggcatatga cgatatgact tatgatgata catcttatga ggaacaacca
    15541 gatagcgctc attcttccag gatccaacgc tatcattgat aggcacaaaa tacgagagag
    15601 gctgtgacaa ccgtcgcagc cttttttggt ttattgcaat tgtctagcat cttctgtaaa
    15661 atagaaacgt tagagaaaat cttgtatcat agaaaattaa gaaagaaata agaacgcagc
    15721 tcggtaccaa aaatatggta aaatggcact tgactgtaat tcactgaaaa tcgcttggag
    15781 gaacacaaat gtttaaagat ttgtatttat ttatgaagga catttatcag aacagaaaat
    15841 tactgctaca gttttcgatc aatgacttca aatcaagata tgcaggatcg tttttaggaa
    15901 tcttatgggc gtttatcaat ccgctattta ctgtattgat ttattggctg gtatttggtg
    15961 ttggactgaa agcacaaatg atcgatgcaa aatacccatt tatcgtatat ttagttaccg
    16021 ggatgattcc ctggttcttc ttctcagatg catttgcatc aactacggtt gtttttcggg
    16081 aatatactta tttagtaaag aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa
    16141 tcctatccaa tctgtacacc catttattct tcattctgat tggcatagct gtcgcgctta
    16201 taaatggtat atatccttca gcaatgtctt tacagctgat ttattattta ttttgcatgt
    16261 gtgctttctt gacaggattg acttggctga cggcatcgat ccaaccattt ttacctgata
    16321 tcatgcaatt aattacgatc gtgctgcagt tgatcatgtg gacattaccg atattatgga
    16381 gccctaatca gtttgattca ggtcttgtaa gcatattgaa attaaatccg ctgtactatg
    16441 ttgttcaagg ttatcgtgag tcatttttaa gtgaagcttg gttctgggag catggaacat
    16501 atactttgta tttctgggtt ttcacgttag taatgttcgt aatcggctct gcagtgttcc
    16561 gtagattgaa accacatttt tcagatgtgc tgtaaggaga atagctatga cagaatatgc
    16621 aattagacta aaagatatca caaaatctta taatatgtac gcaaaacctt cggatcgatt
    16681 tcgtgaagcc ctcaatcctt ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa
    16741 cgtgaatgta gaaataaaaa aaggtgagat gatcggtttc gtgggagaaa acggatcggg
    16801 aaaatctaca atgttgaaaa tcattacggg agtactgaca ccaacatccg gcacgatgga
    16861 aatcgaagga aaaatctcag ctttgctaga actaggatca ggattcaatc ctgagtattc
    16921 tggctatgaa aatatctatt taaatggaat ggttctagga ttttcaagag aagaagtcga
    16981 tgaaatggtc gatgatatca ttgaatttgc tgacatcggc gaccatattt atcaacctgt
    17041 aaaaacctat tcaagtggga tgttcgttcg tctagctttt gctgtagcga tcaatgtgaa
    17101 tcctgatatt ttgattgtgg atgaagcatt ggcagtagga gatcttgaat ttcaactaaa
    17161 atgtatggaa aaatttacag aaatcaagaa ctcgggaaag acgattttat tcgtttctca
    17221 tgatattagt tcgattcgcc gtttttgtga tcgttcctat tggttgaaaa atggaaaagt
    17281 tgtcgaatat ggtgatacga tggaagtaac gacgaattac gaaaactttt tgaagaaaaa
    17341 atcagttaaa acagtagatc gaaataaaaa ccaagtagaa cgcttcgcag ctccagatat
    17401 cgtagacgtg acgaaagccg aattgttgga taaagataaa aatccagtgg atatgctgga
    17461 acaaggagaa aaactatacg ttaaagtaac ctatgaagta aaagacgata caatcaaaaa
    17521 tccagtttta ggaattgctc tgcgtacagt cgacaatttt tatgtgtgcg gactcaatac
    17581 tctactagat ggtatcaaga tcccttggaa aaaaggtgag aatatctttt atctggagta
    17641 tgaaaagtta ggtttgttag ctggtgaata ttattttgac gttgcggtct ttgaagaaaa
    17701 tgcaacggtt cctttggtat acaagacaaa atacatgaac ttgtttgtca gcggttctta
    17761 tatcggagaa ggcatcgtgg tcttagacca taaatgggaa gaagggactc atagcaatga
    17821 aatatgattt tgaaatggac ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga
    17881 tcaaaccagg aagtaaagtt ttagagtttg gtcctggaaa cggccgatta acgaaacatt
    17941 tgatcggagc aaaacaatgt gaagtgagca ttgtcgagct agataaggaa ttgttcgatt
    18001 ttgtcagcga gtttgcccaa gatggatttt atggagatat cgaaagtttt gaatgggcaa
    18061 actattatgc tggacaaaca tttgattacg ttttgtttgc cgatgtttta gaacatttgg
    18121 tagatcctgg aaaaacgttg aagaaagtta gagaattttt gaatgaagaa ggagaaattc
    18181 taattacatt ccctaacctt gcgcataatt ctgtcatgat tgatttgttc aataatcaat
    18241 tgccatgggc ttcttatggt ttgctagatg aaacccataa ttctttttat actcatgatg
    18301 gcttccaaaa agtttttgaa aaagcaggat tatacatcaa tattgaagat tatttgtact
    18361 tagctgtagg tgatacagaa ttaaaatcaa cttatgaaga attgccggaa gctgtacgtt
    18421 atgatttcaa aatgcgacca tttggcgaag tctaccaata cttcttctca ttgatgaaac
    18481 atccagtagc acaaagttcc attgcgcagc cgcaaaattc taattatgtg aaagtattag
    18541 aagtaatcca gcaaacgaaa caagatgaaa taattcaaca aataccattc aataatttca
    18601 caggggaaaa tgagacacta agtttccatg tatcagaatt agcagaacga atggtgtttc
    18661 gcttcgcgca gcaacctagc tttatcgaat tcagcgcaga agctgctgga gaaaaactca
    18721 catttatcga ttcaaatgct gtagtcaaaa cagtaaatga ttgttatttg tttgatggta
    18781 aagatttacc agagttcttt ttaacggata ttgcagggaa agaagtgact atccattgtc
    18841 actatcgttt tatcggagaa ttgacaccaa caatgaaaga attgctggaa acaatccgtc
    18901 caatggctga agttacaaag cagctttctg agaaaaatga tgaattagaa agagaaaatc
    18961 attatcttat tgagaataat acaagattag atcagacgtt gaaaacaacc ataaatcgct
    19021 attgcacgtt gttggaaagt gatgaatgga caatcaaaca tagattaggg cgacgtaaga
    19081 aagaaacatc gaaaaaaatc caagaaaaag agctttctct atgtattgac gaaaaaatct
    19141 gggatgcaga aacaaagata ttgaaaatca tcgggtgggg aatcgctaat tcggatcgcc
    19201 aaccattgtc ctataagtta tcagccgacc aatcaccatt ttttgaagca attcctgttt
    19261 tacgagaaga agtcaatcaa gcaaaacagc tagctgaagg agcaaaagca ggatttgaac
    19321 tgcggatcct ttgcgagaag gaacgttcat tcttagttga agcagtggct caaaatggtc
    19381 aatcatggat cattgaaatg taaataatga ttaacttata aagataagga gtaaacgaat
    19441 cgtgcaaaaa aaagaaattg ccgtatttat cgatcaaatc actcgtgaac gggccactgg
    19501 cgatttgctt attgtgggct gggcaattga tgaagtgacc aaagaaatcc ctacgataaa
    19561 ggtagaaaaa gaaaatgtga ttgctgaagc aacacatgtc gtaagattgg atatcaatca
    19621 tttatataat cttgatgtta agacccaaag cggttttaag gtacgtttat cagggaaaat
    19681 gagaggaaaa gcaatcttag acttccaaac agcaagacat caaaatggca tagctgtgaa
    19741 attaaacggt aaatatcctt atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag
    19801 attactgaaa aaagggataa attatgcaag gactcatggc gtaaaaaaag caatccgccg
    19861 ggtcaaactg gaattgaaac caggatcgat cgactatgca gagtggatca gtcgtcatga
    19921 gaaacctgat ctgaaagagc aaaggaaaga agtacaagga tttgcctacc gcccattgat
    19981 ttccatactt atgcctgtct ataacgttga aatcaaatgg ctcgaaaaat gcattgattc
    20041 ggtacttgat caaacgtatg atcactggga actatgtatc agtgatgatg cttctactga
    20101 tccagctatc cgaaagtgtc tagaatccta tcaagcaaaa gatgatagaa ttaaagtcgt
    20161 tttccgacaa gaaaatggac acatcagtct ggcaactaat tctgcattgg aaatggcaga
    20221 aggagagttt atagcgctct tagataatga tgacgaatta cctccatttg ccttatatga
    20281 agtggcaaaa gtgctaaacg tacatccaga actggatttg atctacagtg acgaagataa
    20341 aatcgatgca gatgggaatc gctttgaccc gcacttcaaa gcagactggt ctcccgatac
    20401 attgatggga aataattata tctctcactt aggcgtatat cggacaagca ttgttaagga
    20461 acttggcggt ttccgtaaag gatatgaagg ctctcaagat tatgatttgg ttcttcgtgt
    20521 gacagaacaa attcctgcag aacacattta ccacattgac cgtgtcttgt atcattggcg
    20581 gacgatcccg ggatcaactg caagcaatgg agaagctaaa agctacatct atgattctgg
    20641 agtgaaagca ttgacagatg ctctatctag aagaaatatc aagggaagcg tacatcctgg
    20701 cagaatttct ggtttttatg aaatcactta tgatgttttg caagaagact tggtcagtgt
    20761 gatcattcca actaagaatg gctatgaaga tctaaaaact tgtgtggatt cgatcatcga
    20821 aaaaacgtct tatccaaatt atgaaatcat tattgcagat aatggaagta cagatcctaa
    20881 aatgcaagaa ttatttgcag aatacaagca tcagctgaaa gaccgattta tcgtcgaatt
    20941 aattgacatt ccattcaatt attcacgtat caacaatctg gcagcagaaa aagcaaacgg
    21001 caaatacttt ttattcttga ataacgatac agaagtgatt gaacctgact ggatgacagc
    21061 gatggtttct tatgcacagt ttgatcgaat cggctgtgta ggagcaaaat tattttatcc
    21121 ggatgataca acgcagcatg caggtgtatt attagggatc ggtggcgttg caggacatgc
    21181 attgaataac tatgatcgaa cacattgtgg ttactttggc cgattggtca ttgacgtcaa
    21241 ttacttagca gtcactgcag cttgtatgat ggtcaaggca gctgacttca atgctgtcaa
    21301 tggatttgat gaaatattag aagtagcatt caacgatgtt gacttgtgct tgaaagttta
    21361 tgaactgggt cgttacaatg tctatgcaca tcaagcagaa ttgtaccatt tcgaatctaa
    21421 atcaagaggt tatgaagata caccagaaaa acaaagacgt tttgctgggg aaatcaaaaa
    21481 aatgcaagat aagtggcctt cttatattgc tcatgaccca ttttataacg ataatctaac
    21541 taaagaagga atcggcgatt tttcgttgag acctgattga ttaaaagaac tgtattttca
    21601 tttttattaa tgaaaataca gttcttttaa tttaattata tgatttgtca aattaagcta
    21661 gaataaaatg cttcttgtac atagctcaaa aatatttcga ttggtgttct gtaattcaat
    21721 gattttcttg gaatatgatt acgttgattg ctgacactgg aaataaatgt ctgattcact
    21781 tttctaaaat ccattgattt cggcagtcca ttacgacgca gaatcccgtt agaattctcg
    21841 tttaatgggc gttgagaagg tgttccaggg tccgcaaaat atatatcaat atcatgttgg
    21901 ttactaatgg ctttccagtt agaaaattct tttccacagt caaacgtaat agatttaaag
    21961 aagtttttag ggaagcgaga aaaccattga ttaagggcag tttcaatatc taatgcctta
    22021 cggccgttgg gtttaatcgt gataatgact ttagataatc tttcaactaa agtaatgacg
    22081 gcacttttat gatgaatgcc aacgatcgta tcaccttcaa ggtgaccaaa ctcagaattg
    22141 aaatcaggat aattatcagg tcgatcatgg attgagcgct gatactgttg ttttccccgt
    22201 ttttcctgat ggccattggg ttttctttta cctttcatcg gtagtgtgtc aatatcaaat
    22261 attcctttag aaaataaacg ataaagtg
//
LOCUS       DACCQI010000047        22284 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCQI010000047 DACCQI010000000
VERSION     DACCQI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.047"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IVO76_001359"
     CDS             complement(218..1015)
                     /locus_tag="IVO76_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7429330.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IVO76_001360"
     CDS             complement(1012..2196)
                     /locus_tag="IVO76_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7429331.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IVO76_001361"
     CDS             complement(2221..3468)
                     /locus_tag="IVO76_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7429332.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IVO76_001362"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IVO76_001362"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7429333.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IVO76_001363"
     CDS             5448..5762
                     /locus_tag="IVO76_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7429334.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IVO76_001364"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IVO76_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7429335.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IVO76_001365"
     CDS             6447..7091
                     /locus_tag="IVO76_001365"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7429336.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IVO76_001366"
     CDS             7106..7435
                     /locus_tag="IVO76_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429337.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IVO76_001367"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IVO76_001367"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7429338.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IVO76_001368"
     CDS             8423..9247
                     /locus_tag="IVO76_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7429339.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IVO76_001369"
     CDS             9240..9587
                     /locus_tag="IVO76_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7429340.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IVO76_001370"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IVO76_001370"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7429341.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IVO76_001371"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IVO76_001371"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7429342.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVO76_001372"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVO76_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7429343.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVO76_001373"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVO76_001373"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7429344.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IVO76_001374"
     CDS             15244..16011
                     /locus_tag="IVO76_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429345.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IVO76_001375"
     CDS             16001..18043
                     /locus_tag="IVO76_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429346.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18315..18464
                     /locus_tag="IVO76_001376"
     CDS             18315..18464
                     /locus_tag="IVO76_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7429347.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18475..19989
                     /gene="dltA"
                     /locus_tag="IVO76_001377"
     CDS             18475..19989
                     /gene="dltA"
                     /locus_tag="IVO76_001377"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7429348.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19986..21191
                     /gene="dltB"
                     /locus_tag="IVO76_001378"
     CDS             19986..21191
                     /gene="dltB"
                     /locus_tag="IVO76_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7429349.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21236..21469
                     /gene="dltC"
                     /locus_tag="IVO76_001379"
     CDS             21236..21469
                     /gene="dltC"
                     /locus_tag="IVO76_001379"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7429350.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21472..>22284
                     /gene="dltD"
                     /locus_tag="IVO76_001380"
     CDS             21472..>22284
                     /gene="dltD"
                     /locus_tag="IVO76_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7429351.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFY"
BASE COUNT         6964 a         4006 c         4618 g         6696 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttaaaa gtacgagaaa ttataaagaa aaaagcagtg
    18181 atttgatgtg ctatttttat aaaagctatg tgacaaaact gttagtaaaa aaacggtttc
    18241 tgtaagctag acacatgttt ctttttgtca aaattagtta cactttgtgt gaggttatat
    18301 gaggagggaa tccaatgaac aaatggcgtt ctaatccaaa attgaattat tggggaaaat
    18361 tcatcattcg aacaattttt tatagtgcag tactgctgtt cctgatttat ctgtatcact
    18421 ataaaaatat tcaaggagga acttttatct ataacgaatt ttaggaggaa gagtatggaa
    18481 atcaaaacga ttattgaagc aatcgatgat tggggaataa atgaacctga tcgagttgct
    18541 tatcaggcgg atgagacgca tacttttggt gaattgaaac aagcatcaga cgcacttgct
    18601 tattatttag aaaaaaaggt agaaggcgat ggaccaattg tcgtttttgg caatttggag
    18661 tttgaaatga ttgtctcatt tctaggggtt gtcaaatctg gccatgccta tttgccgatt
    18721 gaagagcata cacccaagga gcgtatccta tcgatctttc gggtggctaa accatctatg
    18781 gtcatcagta tcggggactg gatagaagat attccttcgg ttcctgtcat aacaaaaaaa
    18841 gaatttcaag aaatcaagaa gataccagta ggattttatc ccagaaatag cgtaaaaggt
    18901 acagaaaatt tttatattat ttttacttct ggtactactg gagaaccaaa aggcgtacaa
    18961 atcagtcatg ataatctagt cagctttgtt caatggacga tggaagactt tgggatcaag
    19021 cctggtatgc atttcttagc ccaagcgcca ttttcttttg atttatcggt attttctatt
    19081 tatcctgcat tagtttctgg cggaatgctg aagccgttaa ataaagcagt tgttcaagac
    19141 tttcgtcaat tattcgcgac attgccagcc ttgaagttga atgtgtgggt ttctactcca
    19201 tcatttatgg atatttgctt gatggaacct acattcaatg gagaaaatgt gccggaattg
    19261 gacatgtttt tattttgcgg agaagaattg acgaaaaaaa ctgcagaaag tctacttgat
    19321 cgattcccaa atgcacggat atataatact tacggtccaa cagaagcaac tgtagctatt
    19381 tcaagtattc agattgacca gcatgtacta gatagttatg accgactacc gattggctac
    19441 gtaaaaaaag acacgaaagt ttctattgtc caagaaggta aacctgtttc aaaaggagaa
    19501 acgggtgaaa tcattattgc aggaccaagc gtatcaaaag gttacctgaa caatcccgaa
    19561 aagacagctg ctgcgttctt ttgtcaaaat gacttagcaa gttaccatac aggggatgca
    19621 ggtagactag atgaatatgg gcttttattt tatgaaggac gtatggatca gcaggtcaaa
    19681 ttacatggat accggattga actcggggat atcgaacatt atctgctcca agacaaccga
    19741 atcaaacaag ctgtcgtcgt accaaaatac caaggaacaa aagtacagca gctggttgct
    19801 tttgtcgtat tagaaaacaa aacgaaacag ccggattttc agttgactaa atctatcaaa
    19861 gaacaattac tagaagttgt aatggattat atgattcctc agaagttcca ttatgtcgat
    19921 ctgctgcctc agacagttaa tggaaaaatc gatcgaaaaa aattaacagc tgaggtgaat
    19981 cctgaatgat gttacctcat atgatccctt atgcagagcc gttttatttt tttcttttag
    20041 ccattgcgct tgtaccgatc gttctatctt tattgatatg ggaaaaaaga ttaccagttt
    20101 atcaatcagc agtgacactt ttctttttat ttgtcagctt tggaggtaat tattggcaac
    20161 agggagcggc gctgattggg tatgttttgt ggcagacgat acttgtatgg ggctattttg
    20221 tttatcggca aaggaaaaat cagacaggat ggttttttgc cgccgttatt ttagcgataa
    20281 ttccactgtt tttaacgaaa gtgactcctt tttttgaaca tggaaaagca tcgttacttg
    20341 gttttttagg aatctcttat ttaaccttta aatctgtaca aatgatcatg gaaataagag
    20401 atggcatgat caaaacatat aagccatttc gttatatcca gtttctgctg ttctttccaa
    20461 caatctcttc tggaccaatc gatcgctata ggagatttga aaaagatcag ctccatccgc
    20521 ctgttcgtga taaatatgtt gatcttttgg gaaaaggcgt ccaaaatatt tttgtcggtt
    20581 ttttatataa attcattatc ggttattatt ttggtcaagt actgcttcct gtagtgggac
    20641 gcattgcctt acagcatggt ggtatttcat gggcattagt aggttatatg tacgtgtaca
    20701 gtatgtattt gttttttgat tttgctggct atagcttatt tgcagtcggt acaagctaca
    20761 tgatgggata tcagacacca atcaatttca ataaaccttt tttatcatgg aatatcaaag
    20821 aattctggaa tcgttggcat atgacgctgt ccttttggtt ccgtgattat gtatatatgc
    20881 gattgatgtt cttcatgatg aagaaaaaat tttttaaaag caagatcgta atgtcaaata
    20941 tcggttattt tgctttgttt cttctaatgg gtatatggca tggattgacg tggtactaca
    21001 tcgtatatgg actgtatcat gcttgtctga tctgtctgac agatgcatgg atccgattta
    21061 agaagaagca taaagagcag ttgccttcta acaaatttac gcatggctta gccgtatttt
    21121 tgacatttca agcagtatgt tttagcttcc tgattttttc aggattttta gataaacttt
    21181 tttttagcta acgaatagtg acatattaat ttatataaca aaggagaaaa ggaaaatgga
    21241 agaacaaatt ttatcaatct tagaagaaat aacaggaaca gatgaagtaa gaaaaaatcg
    21301 tgatattgat ctatttgatg aagggttgct tgattcttta gcatctgttc aattattagt
    21361 cgagctagac ggagagctgg atatccaagt ccctgtatct gaattcgagc gagaggattg
    21421 gagcacaccg aataaaatca ttcaacaagc aactgcattg aagggttgag tatgaaaaag
    21481 aaactttttg ctattttcgg cccggttgtt ttagctgttg gcttactgtt tgtgtttttt
    21541 acctcctcct atcaagtaga tatctctaat ccagaaacga ttagacgtgc ctcttcttcc
    21601 atgtcagtga acgtgttgaa aggagatgcg ataaaaaatt atgcattatc tgaaaaacaa
    21661 tatattcctt tttttggctc aagtgaatta agcagaatca gtccatttca tccgtctgtt
    21721 ctggcagaaa aataccagcg aaactatcgt ccatttttgc tgggagcacc cgggacacaa
    21781 tcgttaagcc aatatatgat gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc
    21841 gtctttatta tttctcctca gtggttcgtt aaaaatggtg taaaaactga ttactttaat
    21901 acgtattatt ctgaacttca aacatacgac tggttgtttt caatgaagaa agtaacgcct
    21961 gctgatcgct atttagctag aagattatta actttctcaa aagtaaaaga gaatgatacg
    22021 ttgacggcga ttctgcaaac gatcaaaaaa gggaagctac ctttacctga atctttgaat
    22081 cagctgcgca gccaatggaa catgctgaag agagaggatg aagtattcag caatattggg
    22141 ttgaaagatc gacagcaaaa aatcgatcat gagtcaaaac gattaccaaa acaataccaa
    22201 gaaacagaat tatccatttt agcaaatcaa attggagaac gagaaacgac gaataatcca
    22261 ttcgggttga aaaacgattt ttat
//
LOCUS       DACCQI010000048        22240 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCQI010000048 DACCQI010000000
VERSION     DACCQI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.048"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            170..514
                     /locus_tag="IVO76_001381"
     CDS             170..514
                     /locus_tag="IVO76_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7429352.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            515..934
                     /locus_tag="IVO76_001382"
     CDS             515..934
                     /locus_tag="IVO76_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7429353.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1026..1376)
                     /locus_tag="IVO76_001383"
     CDS             complement(1026..1376)
                     /locus_tag="IVO76_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429354.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1342..2673)
                     /locus_tag="IVO76_001384"
     CDS             complement(1342..2673)
                     /locus_tag="IVO76_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7429355.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2929..3495
                     /locus_tag="IVO76_001385"
     CDS             2929..3495
                     /locus_tag="IVO76_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7429356.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3640..3727
                     /locus_tag="IVO76_001386"
     tRNA            3640..3727
                     /locus_tag="IVO76_001386"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3672..3674,aa:Ser,seq:cga)
     gene            4061..5035
                     /locus_tag="IVO76_001387"
     CDS             4061..5035
                     /locus_tag="IVO76_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7429357.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            5360..7255
                     /gene="asnB"
                     /locus_tag="IVO76_001388"
     CDS             5360..7255
                     /gene="asnB"
                     /locus_tag="IVO76_001388"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7429358.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(7450..7896)
                     /locus_tag="IVO76_001389"
     CDS             complement(7450..7896)
                     /locus_tag="IVO76_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7429359.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8124..8273
                     /gene="rpmG"
                     /locus_tag="IVO76_001390"
     CDS             8124..8273
                     /gene="rpmG"
                     /locus_tag="IVO76_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7429360.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            8361..8879
                     /locus_tag="IVO76_001391"
     CDS             8361..8879
                     /locus_tag="IVO76_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7429361.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(8956..9912)
                     /locus_tag="IVO76_001392"
     CDS             complement(8956..9912)
                     /locus_tag="IVO76_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7429362.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10078..10884
                     /locus_tag="IVO76_001393"
     CDS             10078..10884
                     /locus_tag="IVO76_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429363.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            10881..11870
                     /locus_tag="IVO76_001394"
     CDS             10881..11870
                     /locus_tag="IVO76_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7429364.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            11867..12871
                     /locus_tag="IVO76_001395"
     CDS             11867..12871
                     /locus_tag="IVO76_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7429365.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13003..13545
                     /locus_tag="IVO76_001396"
     CDS             13003..13545
                     /locus_tag="IVO76_001396"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7429366.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            13565..14263
                     /locus_tag="IVO76_001397"
     CDS             13565..14263
                     /locus_tag="IVO76_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7429367.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            14284..14496
                     /locus_tag="IVO76_001398"
     CDS             14284..14496
                     /locus_tag="IVO76_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7429368.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            14496..15458
                     /locus_tag="IVO76_001399"
     CDS             14496..15458
                     /locus_tag="IVO76_001399"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7429369.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            15476..15871
                     /locus_tag="IVO76_001400"
     CDS             15476..15871
                     /locus_tag="IVO76_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7429370.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16034..16399
                     /locus_tag="IVO76_001401"
     CDS             16034..16399
                     /locus_tag="IVO76_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7429371.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            16396..18207
                     /locus_tag="IVO76_001402"
     CDS             16396..18207
                     /locus_tag="IVO76_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7429372.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(18269..18436)
                     /locus_tag="IVO76_001403"
     CDS             complement(18269..18436)
                     /locus_tag="IVO76_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7429373.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            18684..19673
                     /gene="galE"
                     /locus_tag="IVO76_001404"
     CDS             18684..19673
                     /gene="galE"
                     /locus_tag="IVO76_001404"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7429374.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            19685..21169
                     /gene="galT"
                     /locus_tag="IVO76_001405"
     CDS             19685..21169
                     /gene="galT"
                     /locus_tag="IVO76_001405"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7429375.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            21193..22173
                     /locus_tag="IVO76_001406"
     CDS             21193..22173
                     /locus_tag="IVO76_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7429376.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
BASE COUNT         7213 a         3802 c         4630 g         6595 t
ORIGIN      
        1 aaaaaaagcg ttagcaacta tgcttaaaaa gcatatcgac tgaataaact aagcattgga
       61 caggctgaaa gactacattt aaaatgaaat gcgaagaaac aatagaaaat aaagttgcct
      121 tagaaccata gttgaatcac ctaacagtcg tgaagggaga ataagagaaa tggcagaaaa
      181 acaaaccgca ggacgagatc aattaggaga ttttgcacca aaatttgcag aattgaatga
      241 tgatgtttta tttggacaag tatggtccag agaagaatta tctgctcatc agcgtagtat
      301 catcaccata tctgcattaa tttcaggagg aaattttgaa caattgccag ctcatctgaa
      361 gattggcaag caaaatggca ttactagtga agaaatagca gaaatcatta cccatttagc
      421 tttttatgta ggatggccaa aagcttggtc agcttttagt ttagcaaaag aaatctattt
      481 agaagaaaat agcgaagata agaaagaggc gtaaatgacg acaaaacacg aagaaataca
      541 caatggcgtg atttttccag caggtgaaaa aaacgaagct tacgctcaag tatttaccgg
      601 gcaaagctat ctgaatatgt tagtgaatga tcccgaagtt tctgttggtg taggtaatgt
      661 aacttttgaa ccaggttgtc gcaatcactg gcatgtccat aaagacggct atcaaatctt
      721 acttgtgacc ggaggagaag gctggtatca ggaagaagga aagccagcaa gacgattaat
      781 ggctggagat gtcgtcgtaa cgcacaaagg gattaaacat tggcatggtg cgacaaaaga
      841 cagttggttt gaacatttgg cgatcactgc tggtaattca gagtttttag aacctgtaac
      901 agatgaagtg tatgaaggat tggaaaatca ataatgtcaa gcttgaacag ctcctgaaag
      961 tagattttaa tcgaactttc aggagctgct tttttgattc agtcttttat aaatagaaaa
     1021 tagggttaat aataggaatg agaagcacgt cgaaagatct taatattgat aatcggctgg
     1081 gcatacatga tttccacgtc atcaatcaaa ctacctaaca gatgataatc ttcccgatca
     1141 tgtcggggat tacctaatag ttcttgataa tagatttcca tactagggtc ccgaggattg
     1201 ttcagcaaat cagcaagtac atctaaatct tctggctgag tttctgccgt accctgtatc
     1261 tctatgaaaa gcccttcctc agcaaattct actccaatac gtatttcagt tatttcatgg
     1321 gaatataaat aagtaagaag ctcatcgatg attctttttg ataattcttt ttccaagtgt
     1381 ctttttttcc tttctgacag cggtaaagtt atctagtatc ggtacaagga tacctgcaac
     1441 gaaaccagtt gaaaaaccac tgttatatag atttagtcct ccgtgcaaag tgctgacgtt
     1501 atggacaaga gcaatatgta gtacaccagc tattacaccg taaaaactgc cgtaaaaacc
     1561 gcttatgggc gcgagacctg tcccaaacac agctgcaaca agcatgccag tactggtgat
     1621 ctcatgaaaa gttccaaaca aagaagcaat aaatattccg actagtatcg gaaaactgtt
     1681 ttttaaatga caaccaaaag cactgaaacc caccgcagat aaaatagcac cgatgacagg
     1741 tccatttaac tgaccgccaa ccagcaaaac ataagaagac agcatcagac caactaagcc
     1801 tatgttgatc aaagtagcac caataccaga attagcaatg aaatcagttg tcaattttcc
     1861 agatgaatcg aaaatttctc gaatccccaa aagccgaaag gaattataat agaaaccaat
     1921 tccgactgtc agcgaaaaaa agccaaaaat aatccaaatg agaggaaagt gatattcatt
     1981 gaaaaccaat gtattttcga cgattgaata gctgaataac ctcaaaacag cagtaaaagt
     2041 catcgcaatc aacccagagg tgaatccaat attataaagt gtgaatccac gatggaaaac
     2101 taaagtttga gcagctaagg gcggaagaag tagaccaatt gctatgccag ctaaattccc
     2161 caataaaata cctatcagta aaggaaaacg cataccaaaa gtgatataac taacgaccgg
     2221 tgataatgcg ctaccaaaca gtccgatcat gatgtactga gaaaaaggtt tattgacaaa
     2281 tacagcgtat agatacacac ctaaaatgat tgagatagaa ttataaaggt ttttgccaaa
     2341 aaaagagaaa ccggtaactg ttagaatcga agcaaataag ggaccgttta aaatcacctt
     2401 gtgccgataa gctaaaagca cagaaagtaa ggtcaaaatc cctacattta tgaatgcgct
     2461 tccgaaaccg cctaaagcaa aataatctgt caaaagattg cttggcgagg taagaatagt
     2521 tagcaatcca ctccaattat cagtaggagt attagaaagc aagccaataa aaagaaaaaa
     2581 aacacctgta aaaccaagat aaaaatactt tcttttctgt gaaacgattc gtttttgagg
     2641 atgaatataa ataacgtcag tttcgttttc cattacacac cttctttttc attttcttta
     2701 actataactt gaaatgaaag atttggacaa ctatcattta ggtcaaaact aaaaatgtaa
     2761 gggaacccat tgaatttaac caaatttttt taattttgca aaaaaaagtt aaagaagact
     2821 gaaaaaaaat ttaaaatata acgttttgag taaaatgttt gcaaaaaatg ctaaaaatag
     2881 ggtacaataa aacaacatat actgcagtaa agaagtgggg gagttttaat gagaagaaaa
     2941 gtctatacca aggatcaaat tctaaaagct gcttatgatg ttgttgcaaa ggaagggttt
     3001 aaaggattta ctgcgagaaa tattgctaaa aagatgggga tttcgacaca gccgatttat
     3061 ctggaattca aaaacatgga agatttaaaa aatacgttgc tggattctat ctttgaacaa
     3121 ctaaaagaag atgttttccc tattgaacgt actggtgata aaatcattga tctcggtttg
     3181 aactatatct actttgcaaa aaagcatcat agtctttaca tggcattgta cattgatgaa
     3241 catggggacg gaaagaaaat gcatgatttt tcatacaatt atttccgtga aatagttaaa
     3301 gaagatgaaa gatacaatca attatcggat gaatatgtcg atgctctgca tactggtaca
     3361 tgggtagcag tgactggctt agcgtcattg atgtcatcaa acatcatgca tccaacagaa
     3421 gaacaaatca ttgcactgat caaacggaca attgactcga ttttagcatt agataatcct
     3481 gaaataatca gatagtgttc tatcagtatg aatttgatcg gtaagtccgt tggctgcaaa
     3541 ctgtcaatgg acttttttgc taaaaaaagc gatttctgtt atttatctat tagaaatagg
     3601 cttgacattt cgtgaaaaaa gtttaaaaat agtacttacg gaaggttggc agagtggtaa
     3661 tgcaccggac tcgaaatccg gcgaaccggc taataccggc gcgcaggttc aaatcctgta
     3721 ccttccttat atcaacgatt agaggttgtg aagaagctgt cctgcatcaa gtaataagaa
     3781 cggccaaaca aaaatagctt ttcctatttt ttcgtttgat tgggcttatt accgcagatg
     3841 caagcttttg aacaccgttt gttcggaata aggggctgtg acaagacttt tgtcacagcc
     3901 ccttattgtt gtacttttaa cctacaatat tttttgattc cccctgagta ctatttttta
     3961 taaatgctag taaatcaatc ttctttggcc tttttttgat aaaaaaaata ctagatgtaa
     4021 gataaaagtg taaaaacgat ttgttttagg acgggaaacg atgtgtaccg caatcactta
     4081 tgcaacgaaa gaccattatt ttggaaggaa ttttgattat gagatgtctt ataatgaagt
     4141 ggtcactatt actccaagaa attatcgttt cgattttaga aaggtaaaaa atttagataa
     4201 acattatgca atgataggaa tagcagctgg ggtatccaac tatccactct attatgaggc
     4261 tacaaatgaa aaagggttga gtatggcagg actgaatttt cctggcaatg ctgattataa
     4321 agaattacaa gaaggaaaag ataacgttgc accatttgag tttattccat ggatactagg
     4381 gcaatgctca accatagacg aagctaaaga attattggct actatcaatc tagtcaatat
     4441 cgattttagt gaaaaactac ccttgtcacc tttacactgg ttattagctg ataaagaaaa
     4501 atcgatagta attgaaagta tgaaagatgg acttcattta tatgataatc ctgtcggtgt
     4561 gttgacaaat aatccgccat ttgattatca attatttaat ctgaacaatt atcgctcgct
     4621 atcaaatgga acaccagaaa atcatttttc aaaccagatc agcttgaatg tttatagtcg
     4681 tgggatggga ggattgggct tacctggaga tctctcttct gtttctcgat ttgtaaaagc
     4741 cacatttact aaaatgaatg ctgcatcagg tgattcagaa tctgaaagta tcagtcaatt
     4801 ttttcatata ctaggctcag tcgagcaaca aaaaggagta tgcgatactg gagaaagaaa
     4861 atacgaatat acgatttatt cttcttgctg taacgttgat aaagggattt attattatcg
     4921 tacgtatgaa gacagtcaaa ttactgctgt aaatatgaat aaagaagact tggatcgtca
     4981 tgaattggtc agttatccag tcataaaaga gcaacagata aactatgtca attaattaat
     5041 gaatagatcc gtttggtatc atattttctg atacagtcat aattagacac gatctttagg
     5101 tcgtgtcttt ttttgctggt ttttttggaa atagcatcca ctaatatttt tttaacacga
     5161 agagaataag gtagcctcaa aaaagaaaac atggtaaatt agatattggg actttatttt
     5221 ctgtaaaaac agaaactttc atcttttgtt ttcacgtgac gaaattgtaa gagagaaaat
     5281 attcgtccgc tatttattct atgataagat gcatatgaat gaaataaaca gtaattttat
     5341 attaggatgt gaaagttaaa tgtgcggtat tgtaggtttt gttaacgaca aggacaacaa
     5401 aaaaacaatt attaatacaa tgatggatcg aatcgttcac cgtggaccta atagttcagg
     5461 agaatatatc gataaacatg ttgctttagg attcagaaga ttaagtatta ttgacttaga
     5521 gggcggtact cagcctattt acaatgaaga tcggacaaag atcattattt ttaatggcga
     5581 aatctataat taccagcctt tgagagaaga attgattgca gctggccacg tttttcaaac
     5641 acatgcagat acagaagtcc ttttgcatgg ttacgaagaa tggggaacag aattattgca
     5701 aaagatccgc ggaatgtttg cttttgctat ttgggataat gaaaagaatg agttattcgg
     5761 tgcaagagac cactttggga tcaaaccata ttattatgca gaaatgaacg gaacatttat
     5821 gtttggttca gaaatcaaaa gttttctgcc tcatcctgat tttaacaaag aattgaacaa
     5881 agaagcgttg aaaccttata tgactttcca atattctcca ttaaacggcg aaacattctt
     5941 caaagatgtc tatcgtttgc ctgaaggcca ttattatacg tataaagatg gaaaaataga
     6001 cattcagcaa tattgggatg cagattttga aacaaaagaa acgcattccc gacaagaatg
     6061 gatcgaaaaa atcgacgaaa ccgttcaagc ttctattgaa gcacatacgg tcagtgatgt
     6121 agaagttggt tctttcttat ccagcggggt tgattccagt tatgtaacct ctgtattgaa
     6181 accagatcat tctttctcaa tcgggtttga tgataaaacc tataatgaag cgattgaagc
     6241 cagaaaacta accgaactat tggacttaga taatacagca gcagtgatcg acggagacat
     6301 gtcgttcaaa gcattccctt tgatccagta tcacttagat gaacctgatt caaatccttc
     6361 ttgcgtacct ttgtactttt tagcaaatct tgcttcacaa agtgtgcggg tagtccaatc
     6421 tggagaaggg gcggatgaac tgtttgcggg ataccaaacg tatggattcc atacgaattc
     6481 aaaattcatc cgtgtgattg ctcaaggatt gaaaaaatta ccaaaaggaa cacgctataa
     6541 tttaggacgt aaaatcggaa aaatgaaaaa tttccatgga cggatccatt tatatgaatc
     6601 acttgctcct gcaaaagaat attttatcgg tcatgctcga gtgttcgaag aatcagaagc
     6661 atcagaagtg ttaacaccaa aatatcaaac agcaccatct gtggatgaaa tcatgactgt
     6721 tcattacgag aaaacagaag gaatcaaaga tgaagtcaat aagatgcaat atgtcgatct
     6781 tcatcaatgg atgccaaaag atatcttgtt aaaggcagat aaactttcta tggcaagttc
     6841 tttggaagtc cgagtacctt tattggatat cgaagtcatg aaattggctc aacaaatacc
     6901 aagtaagttt ttattgaatc aaaacaacac aaaagatatt ttcagacaag ctgcgaacaa
     6961 acatcttccg gaagaatggt cgaatcgtgt gaaattagga ttccctgtgc caatcaaagc
     7021 ttggttgaaa gaagaacacg gctacgaaca agtcaaagca ttattcgaag cagattttgc
     7081 aaaagaattt tttgatcaag aaaaaatcat gaagttgcta gatgaccatc acgaaggacg
     7141 taaagaagag caaagaaaaa tctggacaat cttcagtttc ttgacatggt ataaagtcta
     7201 ctttgtagat gaaacgattc cgcaagcaga agcaatcgac tatgtgactg tttgatgaaa
     7261 atcaaatatc aataaaaaag aggttttctc ctaccgttta acactgagtg ataagaaaca
     7321 atttcaaaaa acagctcttc ccgttttata gatattatga cttatggttc ggagttggca
     7381 gaggaacacc attgtttcga acataagggg gttgtgacac gattttcgtc acaacccctt
     7441 tacatatgtt caataatcga tttctttttc ttttgtccca tcttttttat aaatgaacaa
     7501 agttgattct ttgttttgtg ctacttgctt tcctttttcg attgcttctt tttttgtatc
     7561 aaaatggttg cttgcctttt ttgccccttt agatttcacg atccattgtt cttcctcata
     7621 ttctacgatc acatctgaat caagcagttt tccagcacga ggattcgtat cgtgttcatc
     7681 cgtcttgctt ggattttttt ctttttcgaa agttcgtttt tctgattcac tagcatcttc
     7741 ataccatttt tttgcttgat caatggcaat tgggatcgca cggttatcag ggtacccttc
     7801 atctaataat gcatttgcga tgtcgattgc tttttttctt gtcaatttat ctaagttttt
     7861 cattgatgca ggaaagtcct tcatgttcca tggcatatta gtatccccct ttattaaaaa
     7921 acataatcta tataaacaag tgtaagggta tttatacaaa aagaaaaaga ttttgtttga
     7981 gaagtaaaac cgttgtattt atcgtcaagt agttttactt aacaaaatcg tgaaaaacac
     8041 tagcaatcta gaaaaaatca tggtaagata atcaagtctg agagtcatct tagaggattc
     8101 ttgtaaaaat gggagggaaa aacatgcgcg taaacatcac tttagagtgt acttcttgta
     8161 aagaacgtaa ctatctaaca agcaaaaaca aacgtaacaa tcctgatcgt ttggaaaaac
     8221 aaaaatattg cccacgcgaa cgcaaagtta ctttgcaccg tgaaacaaaa taatgttttt
     8281 aaagttctca attccttgct ggtcaaagaa ttgagacttt ttttatctca aaacatctaa
     8341 aatcataagg agcggataat atgtttattt ccatgtgggc acaagataaa aatggtctga
     8401 tcggcaaaaa tggtttgtta ccatggcgat tgccaaatga tatgcgtttc tttcgcgaac
     8461 acacaatgga tcgaatttta gtgatgggaa gaaagaccta tgaaggaatg ggggatcttt
     8521 cacttcctta tcgccatatc atcgtgttga caacgcaacc aaattttcgc acgaaagaaa
     8581 atgcggaagt aatgcattcg atcgatgaac ttttgtcgta tagtcaaacg atctcagaag
     8641 atatctatgt ttcgggtggt agtcggattt ttcaagaact tcttccttac actggtcgga
     8701 tctggcgaac attgatcgat agcacgtttg aaggcgatac gtatattggg aatattgatt
     8761 tttcagactt cgcccttata gaggagtatg aggggataac ggatcaagaa aatctatatg
     8821 cccatcgttt tcaaaaatgg gaaagagtaa aaaatagggt tgcttcaaaa gaagaataaa
     8881 atctatgcgt ctagtttgtt aataagaaaa aaagcatctc acgactaatc gtgaagatgc
     8941 tttttttagt taaaatcaat ttagtttttc tttgatatca ttgaccattt cctgactggc
     9001 aataggtccg ttatatagcc aactagattc aggtccgaat tgcagtacat gattgtctgt
     9061 cactgctttt agattcttcc agcttggttc attgaacatg gcagcatctt tgtcactgtt
     9121 cacaaggatg atgtagtcag catctaattc agctagcttt tcagaggaaa cagcagacca
     9181 gtcagaagtg gcttttttgg agacctcttc cactagatca ggaacaccaa attccagatc
     9241 gtcgtatacg attcttccac ttgaccgatt tttgctgacc ataaaggctg agttgttggt
     9301 cacccacaac actgcagctg tcttgttctc gattttatct gctagttctt ctcgagtttt
     9361 cttgatggat ttttgataat cggaaatgat tttttctgcc tctttttctt ttgccaaagc
     9421 tttgccgaca tcttttagtt gttcttccca tgtgacatct gttccgtttt ttacaacata
     9481 agttggcgcg attttgctat attgttcgta tttcccgcct tcgacagtag cggaagaaga
     9541 aatcaacagc aaatcaggct caaagcttaa cactttctca taaggaaggt cataagaaat
     9601 cgttgggaca tcctttaaat catcttgcag ataatcttga atggaaccac tgccaacagt
     9661 ccactgggcg ataggttttt cccctagtgc aatcagatag tcttctaagt aactgccgat
     9721 gatccgtttg ggcgcgtttg gaatctctac tttatgtcca agtgtatctg tgactgtatg
     9781 agtactattg gatgaagtcg tcgaattggc agttgtagct gtttctttat ctgaagaggg
     9841 ggagcaagcg ctcagtactc cagccagcag caatatgcta gataatgtga atacttttgt
     9901 ttttaatttc atttctttcc tcctaacata attgagaatc tttctcattg gttattattc
     9961 ctgatatttc gaacgctgtc aaccgcttca tttggctata gatatgaaag tagcgcttga
    10021 cgaaatgttg aaacagcgtc ataattgaga atgattttca gcgaaaggat gggtgaaatg
    10081 acgagattaa gagcagctga cattgcagcc ggctacgaaa acaaaaaaat cattgaaaac
    10141 atgtctgtta cgattcctga agggaaaatc actagtttga ttgggccaaa tggaagtgga
    10201 aaatctactt tattgaaaag ttttacccgt atactacctg tgaaatcagg aaagatcatt
    10261 gtcaataact ctgatatctc tacatacagt tcaaaagaac ttgcaaaaaa aattgcatta
    10321 ttagcacaaa ctagtgaaca ccctacaggt atgacagtag aagaagtagt atcttacgga
    10381 cgataccctt atcaaaaact gttcagtggc atggacacgc aagatcatga agcaattgag
    10441 tgggcgttgg atgctacagg tctagctgga tttcgtatgc gggatatcgc aacgctgtct
    10501 ggtggacaaa cccagcgtgt ctggatcgct atggcgcttg ctcaggaaac agatatcttg
    10561 atcttggatg aaccgacaac atttttagat ccggcacacc aattagagat cctacagtta
    10621 ttgactgaaa tcaatcaaat gaaacaaaca acgatcttga tgtcgattca tgacattaat
    10681 catgcttcac gtttttcaga ttatttatta gcaatgaaaa acggtaagtt gatcacagaa
    10741 ggtacacctg aagaagtgat cacaaaagag tgtatgaaaa aaatatttga gattgatgca
    10801 acgattgtca cgcttccaag aacaaataaa ccagtttttc taacctatga tttgttgcca
    10861 aaagagcagg aggatcaatt atgaggacta tgaagaaaaa tagactcctg cttttggtct
    10921 gttttgttct tttgattatt ttttttgttg ctctttcaaa tggcgcagcg actgtgggaa
    10981 aagcagattt ttggcaggcg ttgtttgatt ttgatagatc aaaacaaagt caccaaatca
    11041 tacgaaatat tcgtttgcct cgtgtggcgg gagcatttct tgtagggagt tgttttgcat
    11101 tgagcggtgc attgatgcaa ggagtgacaa gaaatccttt agcagattca ggcttactag
    11161 gaatcaacgc gggtgcttcg ttggcaatgg cccttagttt tgctttttgg cctaaagctt
    11221 ctgatttttc tgtgtttcta atgtcattgg caggttcttt tgctgtaact ttgctcgtat
    11281 ttggtttttt gcagttttcg aaaatgggca tgaatccttt acagctgatt ttagctggcg
    11341 tagctattag ttcttttttt actgctatca gccaagcact tacccaattc tttaatctgc
    11401 agcaggatct tgctttttgg tttgtaggcg gagctgccaa tatcacatgg aaacaattag
    11461 cagtattgtt tcctttatat atacttgctg ttttggtttc tttttcatta ggaaaagcaa
    11521 tatctttgat tcatttgagt gaaacacatg cattagctct tggaagtcat cctaaacgaa
    11581 cgcggttgat tgttttctgt ctagttgcag tgttagcagc cattgccgtt tcgttagtcg
    11641 gacctgtttc atttattggg ctgttggttc cgccagttgt tcgcggaatg gttggaacgg
    11701 actatcgtta tgttctacct gtctcttttt tagttggcgg tggattggtt atgttggcgg
    11761 attttacagc gaggatgatc aatccccctt ttgaaacacc ttttggatta gtcattgcat
    11821 tgattggcgt tccgttttta ctgatacaag taaggaggga acaacggtga aaaaaatata
    11881 ttggacgttc tatttcttgc tttttgtact tgtcatcctg tctataggta gtttaatgat
    11941 tggtacgcct tttatttacc tggatcagct ttttgccacg ttcataggga aagaatcatc
    12001 ttctttggcg ctgattgtta gagaatttcg agttcctcga ttactgatca gtttattagc
    12061 aggagcttgt cttggcgttt ctggttatat cttgcaagga gtaacaagaa atgaattggc
    12121 agattctagt atattgggca ttaatgccgg agcaggattt ttagtgatgc tttatttagg
    12181 tttcttttcg cactattcgc tgccgtttct attgctagtt gtagcctttc taggtggtgt
    12241 tttagctgct tgctgtgtct atcttgcggc ctatgatcga aatggctttt tgggtatgaa
    12301 tcgaatctta ttgtccggta ttgccgttaa tgccggcctt tctgcattaa cattgctatg
    12361 cacgatccaa ttatctaagg aaaattatgg atttgtgaat gcatggttag cgggatcaat
    12421 ctggggtgcg agctggcctt atgtttgggc attgcttcca tgggcggtta tcttaattcc
    12481 gctaggagga ctttatgcac agaggattgt attgctatca tttggtcaag aacgcgcgca
    12541 aggattaggc cttaacgtgg ctaaaagtca aaaaatcctt ttgttgttag ctgttgcttt
    12601 ggcaagcggt agtgtagcgg tgacaggtag tttatctttt gtaggactac tagctcctca
    12661 tgctgcgaag ctagtcgtac gtaaagaaaa taactggacc tttgttctga gcggtttgtt
    12721 cggttccatt tttctgctta gtgcagatat ggttgctaga gtgattctac taagtgggga
    12781 agtacctaca ggaattttga ttgccctttt tggtgcgcca tattttctgt atgcactttt
    12841 tatcagacaa aaacacgcgc tgaacagttg aaattcatta aaaaaggaag gccaatcatt
    12901 tctaggtctt ctttttattc tatttatgca tttatttgaa agttcgattg ctatttttct
    12961 ttttttccta taccatttta tgagagaagg aagggtgagt ttttggacca attgaaagta
    13021 agattacgcc ggaaaggaat cgaaaagctg aaaaatctgg ccaaaagtgg caagaaaaag
    13081 gaaaaagaac agcgaatcta taaacagcta ttcgccagcg agtactggaa gtcagcaaaa
    13141 gtgataggga cgactttatc gaatgaattt gaactggata cacagccggt tatccaacga
    13201 gcagaaaaag aacaaaagaa aatagttgta cctaaaactt tgccgaaacg tcagatggcg
    13261 ttttatgaaa tggatgaaca gtccgttctt gtacgttcga gttttggtgt tcttgagcca
    13321 caatcagaga agctctattt gccggagatg atcgatctcc tcattgtacc aggtattatc
    13381 tacaatagag ccggttatcg aatcggtttt ggcggtggtt attacgaccg gtatttaaat
    13441 aattttcata aaaaaacatg tagccttgtt tttcgtgaac aattgaatga acaatggcaa
    13501 ccggagagct ttgatcagaa gatccaacaa ttgtttattg actaaaatga aatgggaggt
    13561 tttcatgaat tatcaacagc aaataaaatt gcgtgcatgg ttaagacgac cgttatggac
    13621 ctatgctttt ttagggatac agacagcggt cttcctcatg atggaattat ttccgcgact
    13681 tgagatccct tattatgccg gaatgtatgg tccttatctt gttcacttca atgaatggtg
    13741 gcgtttagtg acaccaatct tcatccattt tggactgatg catttcgtaa tgaactcact
    13801 gatactgtat tttatggggc agcaaatcga ggcaatctat ggccattggc gttttttctt
    13861 gatttatatg ttcagcggga taatgggaaa tacagcaagc tttgctttta acgaagcgaa
    13921 tgttctgtct ggtggtgcaa gtacttcgat attcggtttg ttcggtgcgt tgtttatctt
    13981 agggtttcat tttaaacata acccagcgat ccagcagcta gtcagacatt ttcttttatt
    14041 tattgtgttg acatttgttt ttggtcttct ggatacctct gtagatgttt ggggacatgt
    14101 cggtgggcta attggcggat tagtgttagg gaatgttttg ggtctaccga aacaacagac
    14161 aagctattcg atacaccaaa ggattctttc tacactagtt tttgtatttc tttttgtaat
    14221 atgtatttta ctgggtttaa aaaaatatgg tttacttgta taatatgaag gaagtgctta
    14281 aagatggaga gcttatatga tgtacagcag ctgttcaaaa aatttggcat ctttatctat
    14341 gtgggtgcac gcatctatga cattgaatta atgactatcg aattgcgtaa tctgtatgat
    14401 agccgtttga ttgatcatga tacatacatg acagcgtggc gcatattaaa aagagagcac
    14461 cgaattgaag aaagccgaaa gaaaggaaat ttgtaatgga caagaagatt attgggattg
    14521 atttaggtgg aacaacagca aaatttgcaa ttttaacacc agaaggagag attcagcaaa
    14581 aatggagcat cgataccaat attttggatg atggtaagca tatcgttcct gaaatcattg
    14641 aatcaatcaa tcatcgtttg aatttatata atatgaaagc cgaagacttt atcggaatcg
    14701 gcatgggtac cccaggaagc gtggacagcg aagctggaac ggttattggt gcgtataact
    14761 taaactggac agaagttcaa tttgtgaaaa aactgatcga ggcaggaaca ggaatcaaat
    14821 tcgctatcga taatgacgcg aatgttgctg ctttaggtga acgatggaaa ggtgctggag
    14881 aaaacgatcc agacgttgta ttcgttactc taggaacagg tgttggcgga ggtatcgttg
    14941 ctggcggaaa tttgattcac ggcgtgagcg gagctggagg agaaatcgga catatcactg
    15001 tggatcctga agggttcgaa tgtacttgcg gtaaacgcgg atgtttagaa actgtatcta
    15061 gcgctacagg tgtggtacgc ctaggtcgtt tcttggcaga agaatacgca ggggattcaa
    15121 aactgaaagc aatgctagac aacggagaag aagtaacaag taaagatatc tttgaaatgg
    15181 ctcaagaaga cgatccgttt gcattgatgg ttgttgatcg cgtatgcttc tacttaggtt
    15241 tagcttgcgg aaacttgggg aatacattaa atccgtcaag tatcgtgtta ggtggaggag
    15301 tatcggcagc aggagaattt ttacgtagcc gtgtagagaa atattttaaa gaatttactt
    15361 tcccgcaagt aagagaatca acaaaaatca aattagcaga gcttggtaat gaagctggtg
    15421 tcattggtgc tgcctcatta gccttgaaat ttgcttaaaa aaacggaggc attacatggt
    15481 tttatgggta attaatatca ttttgctatt gattgtgctt gcaatcatcc tttgggaagt
    15541 ctatctacga gtaatggcca aacgttcagc aacgactttg acagaagaag aatttcgaga
    15601 aggaatgaga aaagcacaag taatcgatgt tcgcgaaaaa gatgtgtttg atgctgggca
    15661 tattttaggt gcaagaaaca tcccttacac tgttttgaaa gattcattag gatctattcg
    15721 aaaagaccag cctgtctata tttacgatca aaagaaaagt ttgagcatcc gtacagcaaa
    15781 taaattacgt aaagcaggtt atcaagagat ttatttactt aagggtggat atgacggatg
    15841 gtctggaaaa atcaaaagta aaaaagtata aaacagatga ttgtatgaca gctttaaaaa
    15901 ccgaatcaat cattagattt tgaccaagat cacatgaacg gcgcaatgga tagttgtgcc
    15961 gttcattttt tttattttgt gctattatct acatatataa gctaagcgaa aaggtagaaa
    16021 gaaggaaaca actatgatca aattaaaacg tgtttacctt gatgctgaaa aagaggatgg
    16081 ctatagaata ctcgtggata gattctggcc acgaggaatt tcaaaagaaa aagcgcagtt
    16141 agatctgtgg ttgaaagaaa ttgcacctag taatgaagca cgcaaaactt ttgaccatgt
    16201 accagaacga tttgcttcat ttgcaaagaa ataccagcaa gaattgattt tcgaagaaga
    16261 aaagaagcaa gctattttta aactcagtca acttatgaaa gaacatccag tcattacttt
    16321 tgtttatggt gcaaaaaatg aacatcagaa ccatgctgtt ttattgaaaa aatttatcga
    16381 aatggaggca tgcaaatgaa aatagggatc attggtgccg gacctagagg attaagtgta
    16441 gcagaaagga ttctccataa taatcaggac agacattctc ttgaactttt tcttttcgat
    16501 cccaatggtc ctggaggtcg agtttggcgg ttgaatcagc caccggagtt attaatgaat
    16561 tctgtttctc aacaagtgac gctttttaca gatgaaacat tgtccagtgg aggattggta
    16621 acacagggac ccaacttgtt tcaatggagt aaaggacctg ctacagatta tatcgaacag
    16681 acggtaacca aaaataagca actaattata gaagaaatca atcgtctgga aaaagatcag
    16741 caatctactc gatgtttata cggactctat caacgctggt ttttctcaaa gttgcaagaa
    16801 gaatttccag cttctattca gtttgtttat tctttagtaa aaaaagtaag caaaagaaaa
    16861 gatggttatc ttttacagac agatcagcaa agctattcta tcgatcagct tgttttagca
    16921 agcggtcact gggaaaatga tgagacagaa gaagaaagag tgtgggtaga ctacgcaaag
    16981 aaaaatgttt tactgtatca gcttccttct aatcctgcag atgtagatct ttcgaagata
    17041 ccgcctaaag aaaatgtctt tttaagaggt ctaggcttaa gtttttttga ttatgtcgga
    17101 ttgtttacag tatcaagagg agggcgcttc gagaacgaaa agggaaaact ggtctatcat
    17161 ccatcaggca aggaaccgat tgtatacagt ggatcaagaa gggggctccc ttattaccct
    17221 cgaggaagaa atcaaaagca aggcggagca atggcatggc cacggttact aacaaaagaa
    17281 aacctggagc aatggcatca gtcgggattt ttgactggcg aaacattttt tgactatctg
    17341 aaaaaagacg ttgagttgtt ttattacaag aaaatcatcg aagaatatca cttgccgatt
    17401 ccaaagaaaa attttgagca tgattttctc tccctctctt cagaggagtg tctcggaaag
    17461 catccagcac taatccctta tagatgggat tggtcgtatt tagaaactcc tcttgcctat
    17521 cagaatgagc ccttttcaga agctagccga atatttattg aagggcagat aaaagaagcg
    17581 gaaaaaggca attgcacagg agcatttgct tcaacatttg atgcattaaa agactggcgt
    17641 gatccaatcc ggcaggctat tgaatggcac gttttttctg ctaaggaata caaagaaata
    17701 ctatggggat ggtttactca tctaaatagc tttttaacga tcggtccgcc aatcattcgg
    17761 acgaaagaat tagcagcact gatagatgcg ggtatttttc atttgatcga accgccattt
    17821 gatattcagc aaaagcacaa ccgctatgtt atttctctac aagataaaga aatcagcagt
    17881 cgctatttga ttgaagctcg attgccacaa acaagctttt ctcgttcaag taatcctgtc
    17941 ataaaaaatt tgaaggaaaa caaacttgtt cgcccttttt cattttcaga taatacagga
    18001 agttatcatt cagaagctat agatgttgag cgaggtacga atcgcgtgat tgatgcttca
    18061 ggggaaatcc agtctgactt ttattgctat ggaatacctg ttgaaggtgt ggattggcta
    18121 actgcagcag tagctagacc ttatacagat ccatggaatc tgagacaggc agataaaata
    18181 gcacaactta tattgactga caaataaaaa tacgcatgat gacttatctg ttttttagaa
    18241 agtcttcatg cgtattttta aataattatt aacgagaaac gcgtttacgc attgcttttt
    18301 tacggttttc ttcgatcatt gcttcttttt cgtcaattgg atcgatcact gtttttttca
    18361 cagaagcaac caaaccagca acggcagcgg ctgttgccag acttcctact aatattccag
    18421 atacaaattt tttcattgaa tccacctcat ttattatttg ttatatctat tatggaacaa
    18481 aagagaaaaa aaggctagtt ttacgtttgt ttgtaaacct ttgcaaaaaa tccttgaaaa
    18541 tgtttactaa atgttttatt ataaatttac catatgaatt tacgaaattt tactttgttt
    18601 gttttcacca taaaagtaaa cttcgttttt taaattatat agaatcaaca aacacaattt
    18661 tactaatcaa aggagtgttt tttatgacaa ttttagtact aggcggagcc ggttatatcg
    18721 gttcgcacgc tgtagaccag ctagttcaaa aggggtatca agtagctgtg gtagataatt
    18781 tgctcacagg tcacaaacaa gcagtacatc cagatgctca tttttatgaa ggagacatcc
    18841 gggataaaga atttctacgt tccgtatttg aaaaggaacc gattgaagga gtaatccatt
    18901 ttgctgcaag ctctttagta ggagaatctg tcgaaaaacc attgatgtat ttcaacaaca
    18961 acgtatacgg tatgcaaatc ttacttgaag tcatgcacga gttcaacgtg aataaaatcg
    19021 tattctcttc tactgcagca acttatggag agccgaaaga gtctccaatc acagaagata
    19081 caccagctaa tcctaaaaat ccgtatggtg aaagtaaatt gatgatggaa aaaatgatga
    19141 agtggtgtga ccaagcatat ggcatgcgct atgtcgcttt gcgctacttc aatgtagccg
    19201 gagcaaaagc agatgcatct atcggagaag atcatacacc agaaacgcat cttgtcccaa
    19261 tcattttaca agtagctctt ggtcaaagaa aagccttggc tgtttatgga gacgattatg
    19321 atacaccaga tggaacatgt atcagagatt atgttcaagt agaagatttg attgctgcac
    19381 atattttagc tttggaatac ttgaaagaag gaaatgaaag caacttcttc aatttaggca
    19441 gcagcaaagg atactcagtc aaggaaatgc tagaagcagc tagagaagtg acaggaaaag
    19501 aaattccagc agaaatagca ccaagaagag ctggagatcc tagccgatta gtcgcttcaa
    19561 gtgaaaaagc gagagaggtt ctgggttgga aacctgaata cacagatatc aaagcaatta
    19621 tcaaaacagc atgggattgg catgtcagcc atccaaatgg ttatgaagaa taggggaatg
    19681 agatatgtca atcagtcaaa cgatttgtga ttttgccaca cttgcgatcg aagctggcgg
    19741 atggatggaa atggaccgct tatatttaca gaatcgtctt ttagggatgg taggagaaga
    19801 tgctctagaa agcattgaag taagaccagt taaggaagaa tctgtagact tattggatca
    19861 gcttgtcgct aaggcaagag aaaatggtgt tctttcagaa ttgccagcag aacaagagat
    19921 ttttgaagcg caattaatgg attttctcac accgccacct tcagtcgtca atgcattctt
    19981 tgcacagcac tattcgaaaa gcccagagga agcaactgag tatttcttta atttaagtaa
    20041 gaaaaatgac tacatcaaaa caagagccat cgcaaaaaac atcaaatttc cggcagagtc
    20101 ggagtatggt gaactggaaa tcacaatcaa tttatctaag ccagaaaaag atccaaaaca
    20161 aatcgcagca gaacgcgagg cggtaaaagt agactatcct acctgcatgc tttgcatgga
    20221 aaacgaagga tatagaggtc gcttgaacta tccagcccgc accaatcacc ggattattcg
    20281 aatgaatcta gacggtgaaa gctggggatt ccaatattct ccttatgctt attacaatga
    20341 acattcgatc attttatcag aacaacaccg cccgatgaaa atcacaaagg aaacatttgc
    20401 tagactgctt cggattactg aagtcttgcc tcattacttt gttggttcga atgctgactt
    20461 gccgattgtt ggaggatcga ttctttctca tgatcattat caggcaggac gtcatgaatt
    20521 tcctatggca aaagcaccga tagaaaaact tatcaaacta gcagattatc cgcaagtcat
    20581 cgcaggagtc gtgaaatggc cgatgtctgt gatccgtttg caatcaacag agaaaaaatt
    20641 gttgattgaa gcagctaact cagtattgga aaagtggaaa gcatattcgg atgaaacagt
    20701 atctatcaga gctttttcag cagatggtac accacaccat acgattacgc caattgcacg
    20761 aagaagggac aattactttg agttggacct tgtccttcga gacaacaatg tttcagaaga
    20821 acatcctgat gggatttttc atccacataa agatgtccaa catatcaaaa aggagaacat
    20881 tgggttgatc gaagtaatgg ggcttgctgt tttaccacct cgtttgaaac cagaactagt
    20941 agaagtagag catttcttat taggagaaga aaaccagatg gctgaatatc atcgtccgtg
    21001 ggcagaacaa ttaaaaaaag aacatcctga tttgacgaaa gatacggtca ccgatgtcgt
    21061 acaaaaatct gtaggtcagg tttttgcacg tgtactggaa gacgctggag tattcaagcg
    21121 agatgaaaaa ggacaggcag cgtttgaacg atttactgca attttataaa cgtaaaaaag
    21181 gaggagagac aaatggcaac aattaaagac attgcgcagt tggcaggagt ttctcctgct
    21241 actgtttcaa gagttctcaa ctatgatcct gatctttccg ttggcttagc cacaaagcaa
    21301 aaaatttttg aagcagcaga acgcttaaat tatacgaaac atcgtaaaaa tgcagcaaat
    21361 actaagcaaa cgcttcgcct gatccagtgg tacgacgata cggaagagct tgaagatctt
    21421 tattatttat ctattcgtct aggaatcgaa aaaaaggcag aagaagcaaa cgttacttta
    21481 ttgaaagaaa cgtgggattc cattagtgat cttccgacag atggaacgat cgcactagga
    21541 aaattcgacg aagctcagtt attagaaatg aaaggatccc aaaatgcctt gcttttggta
    21601 gattctgacg gcacttctca aggaatcgac tctctagttg tcgactttaa gtcaagtgtt
    21661 caaaaagtca ttgaacattt tgtctcccat aaagcgcaac atatcgctat gcttgctgga
    21721 acagaatata cgaagaaaaa ccatcagcgg ctaaaagatc ccagaattat ggcatttcaa
    21781 caagttttgg cagaaaaagg ctacgacgaa ggtccaatta tcgaagcaga tttttctgtc
    21841 gagtcaggat atcaagcggt gaagcagtat ttaaaaacga atccgcatgt tcctgatgcg
    21901 ctttttgcag ctaatgatgc attagcgatt ggcgcactga aagcgattca agaaaaaggt
    21961 ttagtggtac cagaagatat ttctgtgatg ggtttcaatg atgtcagtgt agcgaaatat
    22021 gtaacgccct ctctttctac tgtaaaagtt tataccgaat ggatgggaga gctagcagtg
    22081 gagacgattc ttcaattaag taattccaaa gcaccggttt caagaaagat tattgtggaa
    22141 acagagttga ttattcgaga atcaacgaaa taaaagatgg aaaagctgtg gggaaatgaa
    22201 gtgataccgc agcttttttg tgtttcagga aggggcaaaa
//
LOCUS       DACCQI010000049        21741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCQI010000049 DACCQI010000000
VERSION     DACCQI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.049"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..682)
                     /locus_tag="IVO76_001407"
     CDS             complement(<1..682)
                     /locus_tag="IVO76_001407"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7429377.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYI"
     gene            complement(794..1126)
                     /locus_tag="IVO76_001408"
     CDS             complement(794..1126)
                     /locus_tag="IVO76_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7429378.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1274..2149
                     /locus_tag="IVO76_001409"
     CDS             1274..2149
                     /locus_tag="IVO76_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7429379.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(2231..2812)
                     /locus_tag="IVO76_001410"
     CDS             complement(2231..2812)
                     /locus_tag="IVO76_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7429380.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2818..4077)
                     /locus_tag="IVO76_001411"
     CDS             complement(2818..4077)
                     /locus_tag="IVO76_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7429381.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(4079..5125)
                     /locus_tag="IVO76_001412"
     CDS             complement(4079..5125)
                     /locus_tag="IVO76_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429382.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(5360..5635)
                     /locus_tag="IVO76_001413"
     CDS             complement(5360..5635)
                     /locus_tag="IVO76_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7429383.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(5878..7188)
                     /locus_tag="IVO76_001414"
     CDS             complement(5878..7188)
                     /locus_tag="IVO76_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7429384.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(7370..8599)
                     /gene="rpsA"
                     /locus_tag="IVO76_001415"
     CDS             complement(7370..8599)
                     /gene="rpsA"
                     /locus_tag="IVO76_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7429385.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(8734..9414)
                     /locus_tag="IVO76_001416"
     CDS             complement(8734..9414)
                     /locus_tag="IVO76_001416"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7429386.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     SITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(9463..10098)
                     /locus_tag="IVO76_001417"
     CDS             complement(9463..10098)
                     /locus_tag="IVO76_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7429387.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(10164..11567)
                     /locus_tag="IVO76_001418"
     CDS             complement(10164..11567)
                     /locus_tag="IVO76_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7429388.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(11554..12585)
                     /locus_tag="IVO76_001419"
     CDS             complement(11554..12585)
                     /locus_tag="IVO76_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429389.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            12616..12837
                     /locus_tag="IVO76_001420"
     CDS             12616..12837
                     /locus_tag="IVO76_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7429390.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(13002..13739)
                     /locus_tag="IVO76_001421"
     CDS             complement(13002..13739)
                     /locus_tag="IVO76_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7429391.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(13892..15232)
                     /locus_tag="IVO76_001422"
     CDS             complement(13892..15232)
                     /locus_tag="IVO76_001422"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7429392.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(15469..16992)
                     /locus_tag="IVO76_001423"
     CDS             complement(15469..16992)
                     /locus_tag="IVO76_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7429393.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(17370..17981)
                     /locus_tag="IVO76_001424"
     CDS             complement(17370..17981)
                     /locus_tag="IVO76_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7429394.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(18104..18229)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(18311..19027)
                     /locus_tag="IVO76_001425"
     CDS             complement(18311..19027)
                     /locus_tag="IVO76_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7429395.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(19033..19602)
                     /gene="scpB"
                     /locus_tag="IVO76_001426"
     CDS             complement(19033..19602)
                     /gene="scpB"
                     /locus_tag="IVO76_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7429396.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(19586..20383)
                     /locus_tag="IVO76_001427"
     CDS             complement(19586..20383)
                     /locus_tag="IVO76_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7429397.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(20353..20724)
                     /locus_tag="IVO76_001428"
     CDS             complement(20353..20724)
                     /locus_tag="IVO76_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7429398.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(20743..21630)
                     /gene="xerD"
                     /locus_tag="IVO76_001429"
     CDS             complement(20743..21630)
                     /gene="xerD"
                     /locus_tag="IVO76_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7429399.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6119 a         4651 c         3691 g         7280 t
ORIGIN      
        1 aaatgtagca ctctgtttca ataaatgcat ggattcccgc ggcccgttca tttcccaaga
       61 gcatcttgat gaattccaca tttatacgtt ctacagatat tttacctaat agctcgtggt
      121 tttctttgat ggcgtagaag gtttcttcct ctaatttgaa ccctagttga ctgacaaaac
      181 gcaacccgcg catcattcgt aaggcatctt catggaaccg ctcatgagga ttgcctacag
      241 ctcgcaagat ctgttcttct agatcttgca gaccatcgaa caaatcgatg atttctccat
      301 cttctttcaa agcaaacgca ttgatcgtaa aatctcttct tttcaaatct tctgccaacg
      361 aacgaacgaa ttcgacatga tctggtctac ggtagtcctg ataagttgat tccgtccgaa
      421 atgtggtgat ctcatattgt tcatcatttt ctaataccaa cacagttcca tgttcgatcc
      481 caatatctat cgtttttgga aaaagctgtt tgatttcttc tggaaatgcg cttgttgcga
      541 tatccacatc atggattggc tggttcagca agatatcacg aacacttcca cctacaaaat
      601 aagcttcata tcctgcattc tctaattttt tcagaactgg aattgccttt tggtattcca
      661 gaggtaattg ttttaatttc atttttccaa cctttcctgc gtgtttcttg tcctgagata
      721 aagactcagt tgcatggtga ctgtttgaca agtccgtttc agagtcttca aggccaggac
      781 cacttcatct tgtttactcg tccttacgct caaagcgatt atggtctctt tgattgaatt
      841 tttccatgtt tttttcaaaa acttcagtta agtcgatatt caatgaatta gccatgatca
      901 tcgtaacgaa taaaacatca ccaagttctt ctgctaccga gttaactggt tcagaagcct
      961 ttttgctttt ttctccataa gtgtggttga cttctcttgc caattcacct acttcttcag
     1021 ttaatcgagc catctgagct aatggagaga aataccccac cttgaattgc tggatatatt
     1081 catccacttc ctgctgcata ctgtataaag aacgcttttt atccatcttt ttccctccaa
     1141 aactatctta ccaaaacttt aacgcaaatc ctagataaga gcaattgaga aaatcgaatt
     1201 tccatgctac atttaggaag gaatttctaa atggaaattc tgtgaaaata cataattgaa
     1261 agctggaatt catatggtca aacgaaaaac agtaaaagaa gttcttttta tcattatagg
     1321 aacaagtatt tatgcatttg gtcttgttta cttgaacatc gctaatcatt tagcagaagg
     1381 gggagtctca gggatcacct tgatcttaag agctttattt ggtattgatc cagcttatac
     1441 gactttatta atcaacatcc cattgatttt actaggagga aagatcctcg gtaaacgttc
     1501 cttggcctat actgttctag gtacactttc tttgtcattc tttttatgga tctggcaaag
     1561 ggttccacta gaaatcaatt tacagcatga tctactgatc gtttctctac ttgcgggttt
     1621 agttgcaggt gtaggaagcg ggatcgtcta ccgcatgggt ggaacaacag gcggcagtga
     1681 tattatcgct cggattttag aacaaaatta cggcatcact atggggcgtt ccctattggc
     1741 atttgatatc atcgtcttac ttgcttcatt aacctatatc gacttgaaac gtatgatgta
     1801 tacgttgatt gtttcgtatg ttttcagtcg tgtgattgat tcgatcttag atggcggata
     1861 ttctgcaaaa ggtctacttg tcgtctccaa caaaagcgaa gagattgcac cactactgat
     1921 gaccggtctg caaagaggtg tgactttcct ttctggagaa ggcgcattct ctggtaattc
     1981 aaaaaaaatc atctatatgg tcgttagctc tagtgaatta actgaagcta agaggatcgt
     2041 ccacgagatt gacgaaaaag ctttcctttc aatcatcaat gtgcatgaag tggaagggca
     2101 aggctttact tatttgaagc cacagacaag attactgaaa aaactataag aaaaaagaca
     2161 aagataggct atgaggaaag attccacatc cagctgacct ttgtcttttt tagtactata
     2221 ctatttttaa ctaattgtta tgttcactta cccggttact tttcttttgt gtctgcaaaa
     2281 ggatctttcg gttaacttca tcagataatt tcaaaaaagc atccctatct ccatcttcta
     2341 gtgcttgatc gatttgagca taaagcattt ttagttttgc ctcttcttct ttttcttgaa
     2401 aatatcgttc aattaattca gtatcttctt cactgactga agaatcccaa gaagaaaatg
     2461 gattgtcttc taagatagat agatattctc ttgtctgcca agcattttcg aatatacatt
     2521 ccacatataa aggctttttc caattcaaac ggatttcatg aaaaatctgg tctgtgtccg
     2581 taaattcttt tccctgcaaa aacaatttca tgggttcccc ttcaacagat agatcactga
     2641 tctgcagtcc tcgatccgca aaattcgccc cttcaacaaa gtggacattg cttaaaatcg
     2701 cttcatgatt ggcaagataa ttcaagatcc aaaaaacttc tcttcggcta aaagatacat
     2761 gattgaccat ccacgttaaa aaattcttct tttcccgtac atcaattagc atttttctca
     2821 cctctctgtt aggtcatcta ataatgacag tgcctccata tctcctggtt caagtgttaa
     2881 ataataagtc aataattctt gcgctctttt cagctgccct tcttctctta agaaaaaggc
     2941 atactctttc aagaaatctg gttcatgtga tagttcatga taagcctgtt catagtgtac
     3001 agctgcaacg gtaaagtctt ctaattcatt ataagcatgg gctaggttcc attctgcata
     3061 aggattagca gtttcttcca tctgattgat cacttgtatc acatcttcat atctttcatc
     3121 atccaagtaa agattactta aagtaagtaa tgtttcatct tgtttgtctc ctacttctaa
     3181 agctttcagt aaaagttctt ctgctcgctg tctatcatgc agacggaaag cattttccga
     3241 ggccaggtga taaagttcga cttggtaagg attttctttg attccttctt ctaacactgt
     3301 ttgtgcttct tcaatcattt cttcttcttg aagagcttgt cctagatata gatataacga
     3361 ttgataatgc ggattaactt ctcgaagttc ttccagatag ttgattgcct gctgattttc
     3421 tttcaattga agattcgtaa aagctagctg gaacagtcga tcatctgtcc tttcttcttt
     3481 cagtgcttct tctaaaggta cgatagctgc ttcgaattct ccttgcatac ttaaggattg
     3541 tcctaaacgt tcctgcatcg agattcctga aatttcattt atcccgcttg ccaacaatgt
     3601 ttcataaacc actgctgcct ctttgaaacg atcaactgaa aaatacaatt cagctagcgc
     3661 aaactggatc aatggttcat caggaagcaa cccagctgct tctttcaatt ttgcttcact
     3721 cacttcaggg attccgataa cttggtacaa atcagctatc gccaataagc tttgcggata
     3781 gtaatcactt tgcttatcca ctttttccag gtattcaaaa gctaagtcga tctcattatc
     3841 ttcgattgca atttctgcta acgggatgtt cagtccgtca ttttctgggt attggttgat
     3901 cagttgttca aaaatctgtt ttgcttcgat caaaaagcca atagccagca attcttcgcc
     3961 tagttcagct aatatatctg ctgggtcttc ttttaatgct ttttctaaaa agatttgtgc
     4021 ttgtgacaag tcttcatctg ataaagcttg caacattttt tcgctatttg tattcatttc
     4081 aattccctct tatctcactg acgatttgtt gaaataccgt cattgttctc aataattctt
     4141 tttcgtttgg atgttggtat ccgtctatta tacctatttt ttctaaaaca agtatttttt
     4201 tgcttttttc tgccaaaaaa agaacttttt ccaaatactc catctttgaa atctgttcca
     4261 gccagtggat tggatagcca agcaagtcca gccacacaaa aaagtttttt agttggaaat
     4321 caaaaggata tgcttggaga ttccacacga aatgaaacat cattcctaaa aattttttca
     4381 tactagcaga aagtagatgg ccattttcgg tcagataaaa agcttgttca aacagtttac
     4441 cataatttga caggttttct gcctctaatt gataaaaata gatcatttct tcgatcatcc
     4501 cggcaaacgg ccgagtcgtt aattgctttt tatttggata gtttttatac agattttgca
     4561 aaaaacgatg atcacaaatc attccgcaac agataaaagt caaaaagtca atctgctttc
     4621 cgtccacctg ccgctcgctg attgtttgat cataaacgat gcgttctggc agattgatgg
     4681 tttgcagcac tacacgattt ggtagctgca aaatcgtccg ctgcggtgtt aatgctcggc
     4741 ttagagaacg gatcgataca gggatcatcc atagtgatga atcaagggtt gtatgttttt
     4801 gaaaaaaacc tgccagatcc atgatccctt catttccaaa tccgatgata aacatggatt
     4861 gattttcggg atagcgtttg acgaaatcaa gcaggttctt taattctgtt aaatgattgc
     4921 attgagtgtt ggcacaaata taccagtcat tgtttgcttg atttgcaaat aacgggttga
     4981 tcttatctgc aaacaaatca taatagcgct gattcgttaa aaataagatt ggctgatcaa
     5041 tggaaagttc tcttagctga ctgactagcg tttctccgta aaacactttc gtcaacgcat
     5101 cttctacttg ataatcaagt tccatacgca tccctcctct tcttattttt attttaccat
     5161 tagtaagcaa attctagaaa agataagagc aaagctcacc tcataacttg caaataaata
     5221 aaaattaaat aaaatacatg tgttttctat gtatgacata cggattcgga atctctgtct
     5281 cttatttatt ccttttctgc taggaaataa aaagaaaagc acttgtagcc ctagagccac
     5341 aagcgctttt tcaactgtct tatttaacag cgtcttttaa tgctttacct ggtttgaatg
     5401 caggtacttt gctagctgga atttcgattt cttcaccagt ttgtgggtta cgacctttgc
     5461 gagctgcgcg ttcgcgaact tcaaagttac caaaaccgat taattgaact ttttcgccgt
     5521 tagctaaagc atcttgaata gttgaaaata cagcatctac tgctgcagtt gcatcttttt
     5581 tagttaaatc agtagctgaa gcaacttttt cgattaattc tgctttgttt gccatggata
     5641 gattcacctc ctccacaaga gatatgatag aaatatcatt tagttaaaac tttctgagtg
     5701 gtccagaagt ttaaaaagaa atattgatta acttcaatat tctgttatca cgatatcata
     5761 tgaaccgctt aatagcaagg tttttaccaa ttttcacatg ctttttttag tcatagaaac
     5821 gtaacaaacg aaactgtctt tttcttttga aaaaaaaacg tcgatatggt aaaatgccta
     5881 ttttctccta cgagggataa ttcggatgga cgtcccttca aaagtaaatg cttttctgat
     5941 ttgattttct aagaaacgtt cataagaaaa atgcatcaat tcctcttcat tgacaaaaac
     6001 gacaaatgta ggtggtttga cagcgacttg tgtagcataa aaaatcttca ggcgttttcc
     6061 tttatctgtt ggtgttggat tgattgctac agcatccata atgatatcat tcaacacagc
     6121 cgaagagata cgaagattct ggttcatact tacttgttcg atcatttcag gcagacgttc
     6181 caaccgttgt ttagtcactg cagaaacaaa aacgattgga gcgtaatcaa gataccggaa
     6241 ttcatcacga atctcttgtt cgaaatcacg cattgtattg gtttctttct ttacaagatc
     6301 ccatttattc acgacgataa taattccacg accagcttca tgggcgtaac ccgctactcg
     6361 cttgtcttgt tcccgaatcc cttcttcagc attcagtacc atcaaaacga tatcagaacg
     6421 gtcaatggca cgcattgcac gcatcacgct gtatttttca gtattttcgt atacttttcc
     6481 gcgtttacgc ataccagctg tatctatcat taagaatttc tgaccacttt cagaaacgaa
     6541 gtgggtgtcg attgcgtctc gggtcgttcc ttcaatatcc gaaacaatta cacgttcttc
     6601 tcctaaaatc gcattgatca aagaggattt accaacattt gggcgaccaa tcaaactaaa
     6661 cttaatcgtg tcgtcgtcct cttcttcagt ctcttcagag aaatacttca ctgcttcatc
     6721 taaaatatca ccaatcccta gtccatggct tcctgaaaca ggataaggat cgcctagccc
     6781 taaggaataa aattcataga tatcgtttct catttctgga ttatctactt tattgactgc
     6841 taaaatcact ggtttattac ttctgtataa aattttcgca accatctcat ctgcgtctgt
     6901 cacgccttcg cgtccgctga ctacacaaat gattacatct gcttcttcaa tcgcgatttc
     6961 tgcttgatgc ttgatctgat ccataaaagg ttcatcgcca agatcaattc cgcctgtgtc
     7021 gatcacgctg aattctcgac ctaaccattc accttttgca taaatacgat cacgagttac
     7081 tccaggagta tcttcgacaa tcgagatacg ctcaccagcg atgcggttaa aaatcgtaga
     7141 cttaccgacg ttcgggcggc caacgattgc aattgttgga tttgccatat tatttcctcc
     7201 tcacaagttt ccaatctcac ttagtttaac gaatgcattg ccaactttca agcatagatt
     7261 gtcaattaaa cgaagtattt ttattcctta ttgaaatact aatcgctttt caaaagaaaa
     7321 actgagacaa aagcagctgc tttcatctca gtcacagata caaaatcaat tatttttcag
     7381 aatcatcggt tgctttttct tctacttctt ctttcaacgc atcacctaaa atgtctccca
     7441 tagtaaatcc agtattttct tctggtaatt cataagactc aacatttttt ggttcttctt
     7501 ctgaagctgg tttttcttct aaagctttga tactcaatgc aatacgatgt tcttctggat
     7561 ggatctctaa tactttcact ttcacttcgt caccttcatg aagcacttca tgtggtgtcg
     7621 caatatgttt atgtgaaatt tgagagatat ggactagccc ttctactcca gggaacactt
     7681 caacaaaggc accgaagctt gtcaaacgtt taactgttcc gtctaatact gtgccaactg
     7741 gagcttttgt atcgatttct gaccaaggtc caggctgtgt gtctttgatt gacaaggaaa
     7801 cacgttcttg ttcaggattg atagacaaca ctttgacatt tacatcatca ttgacagcca
     7861 atacatcact tggttttgct acatgactat gtgaaatctc tgagacatgg actagaccgt
     7921 caattccgcc taggtcgaca aatgcgccaa aatcagtaag acgtgcaaca cgtccactga
     7981 gaacatctcc ctcatggata gttgaaagaa tttctttttt cttttcttct ctttcgtttt
     8041 ccactaccgc tttatgcgat aagatcaaac gattttctga tggttcgatt tcgatgattt
     8101 taaagctcaa tgttttgcct ttatagtctg aaaagtctgc aacaaaatga tcttccacca
     8161 ttgatgcagg gacaaaacca cgtacaccga cgtcaacgac taaaccgcct ttgacaacat
     8221 ttgtaacagg tgcttcgatg accttgccct cttggaaatc tctttcgata tcttcccaaa
     8281 cttttttagc atctaaacgg cgttttgaaa gtagatagct gccgttttct ttatctttgc
     8341 cgatcgtact gataacgact aaatctaata catcaccgac ttttactact tcgctgatat
     8401 cttctaccgg caaagttgat aattcttttg ccggaacgac tccttcaact ccagcccctt
     8461 cgattccgac aatcacttgt ttgtcttcaa tgactaaaac ttcgccctta acaacatcgc
     8521 caacttttac ttcttgtacg cttttcattg cgtcttccat tgactggttg ttctcttgat
     8581 tttgatcaaa ttctgtcata acacttgtcc tcctaaccaa cattctgccg taagtacggt
     8641 actgttttta gtttaattga ttattcaaaa aaaataaagc gatttcgttc gttttccttg
     8701 aaaaaacaat tgtttttcat tttttttctt aatttaaaaa agaagataac cttttttcaa
     8761 aacaacagct tttattgctg caacgacttc tggaatcgat ttgccagtcg tatcgatacg
     8821 aacagcatcc tctgcttgct tcagcggaga gacgtccctt gttgaatcat aatggtctct
     8881 ttcctgtatc tctttcgtca gtgtttcaag atcagtcgga attccttttt cttggttctc
     8941 tttgaaacgt ctttctgcac gctcacttac gcttgctact aaaaagattt tcacttctgc
     9001 attcggtaat acagctgtcc cgatgtctct tccgtccatg acaacgccac cgcgttcccc
     9061 tatttttcgt tggacagcaa ctaattcttc tctgactttt ccatgtgcag agacttcaga
     9121 aacagctttt gtgacatctg gttgacggat ttctaaagtc acatcttttg atgctatgaa
     9181 tacatgctgt ccatcttctt gttgcgaaaa agagatagga tactttttga tcagcgaaac
     9241 tagcccttct tcatctgaaa aagatacatt gttcgtaata gataaataag ttactgcgcg
     9301 atacatggca ccggtatctg tataaatata attcaattct tttgctaata tcttggcaac
     9361 ggtacttttc ccagatgaag caggcccgtc gattgcaata ctgatttgtg tcatgatata
     9421 actccttttt ctcagaaaaa aagccgcaaa gcgcggctat tattatttaa ttcttaattc
     9481 ttgaccaggg taaagcataa agttgtttgg atctaagcca ttcaattcaa acaattgatc
     9541 agtggtgatc cctgcacgct cagccacttg ttttggacct tcgcctgctt gaacagtcgt
     9601 tgttgaaccg gctgcagctg atgaagaagg tgttgtttct gctgcattgc tgcttgaatc
     9661 ccctgcttga taagttgcct cacttgagga agtcggtgtt gtctctgctg caatactgct
     9721 ttctgacgtt acacctgtat cttgagacac agtagattct tccgcgattg tactagattc
     9781 tactgttgaa gtctcagttg actcctcttt tgttgtactt gtagaacttt ctactactga
     9841 tgtcgaagtt tcagaagaag ttgctgctgt tgcattgttg cttgatccat ttctgatcca
     9901 gaaataagca cctgctggaa tagcgataca taagaccagt aaaatcaaaa atagcgaaag
     9961 aaataatgta tttcctttct tttgttgtct ctgagaactt cttgatgaaa cttcatcatc
    10021 tgtatcgtag attggttgtt cccatggttc ttgtgtttcg ttattttctg tagattgatg
    10081 tctatctttt ctgctcactg ttttacctcc taaactctat cgtgtgtatt ataccatagg
    10141 aaaaagtgtc ttgtcctaaa aaatcaatgt tccatgtttt ttttaaataa atttagtaaa
    10201 atcgtttccc aatgaagttg ttcagtctta gaaaaaagct tcctctttgg cagaagttct
    10261 gcttttgctc catcaatatc acaacaattc tgcggctttt cggagagttt ttcgccaaaa
    10321 taagctaaga tgaattttct gcggcagttt tcttcattaa tatagcgaag catctgctgc
    10381 aggcggaatt ctttttcttt ttcctgacgt ttgagccgtt ccacccaatt ttctggtttt
    10441 tcagactgct ggatcagctc catccatttt ttttgcagct catcaaaggg agcttgctct
    10501 gtggcatatt ccaaataaag ctcaaaactt tgtcgctgtt ctctagaaag ttgattaaag
    10561 aaataatgga tacgttcatc cccactttga taaaaaagaa ttgctgcact ttcttcctga
    10621 tctctgcctg ctcgaccgat ttcttgtaca tagttttcta gactgtctgg caaatcataa
    10681 tggaccacat aacggatatc agatttatcg atccccatac caaatgcatt cgtagcaatg
    10741 agaaactgca gctgattttt tacaaactgc tgttgaagtt gtcttctttg agctgcatca
    10801 agaccaccgt gatagtatcc aactgtaaaa cgttccctga ataaatgata caatcgttcg
    10861 acttcttttt ttgttgcaca ataaatgatt gccgctcctt gtgctctttc catgaagtgt
    10921 tcaagttctt gttctttttg ctgcgttttc cgaacaaaaa gcgagatatt ttgccgattg
    10981 actgattgtc tgatctcttt tggtggtcga tcaaatagta catgacgtat atctcttgct
    11041 actaaatctg ttgctgtcgc cgttagtgcc agtgtgaccg ggtttcctaa aagctttcgg
    11101 atttttccta actgttgata ttctggacgg aaatcaactc cccattgaga cacgcaatga
    11161 gcctcatcta ctacatataa cgcaatattt tgttttttca gatgctctaa aagagaagga
    11221 ttagtcaaca tttcgggact caaaaacaaa aatttatatt gcgatagatg acggaggacg
    11281 tatcttcttt cagattgcga aagtaaacta ttgaaagcag ctactcgttt ctcccctcgt
    11341 ttttgtaatt gcgtcacttg atcttccatt agagaaagca gcggtgacac gatgatgacc
    11401 agaccttcca ttaaataccc cgtcaattga tagcaaaggc tttttcccgt tcctgtaggc
    11461 aaaatagcga gagtatcttc tccatcaagc aacgcctgaa taatttcttc ttggccggga
    11521 cgaaatgaat caaatccaaa atattttttt aattcattta ttaacattct gtatcgcttc
    11581 ctttctcaat agatagattt gatacaaacg aaattctcca taatctaatt gtccatctac
    11641 attccattcg ctaaagcgcc actctcgcac ggaatcctta tttggttcca aacgttcgat
    11701 tgtttctgca gagagaatcc actcgtatgg aaaatcatca aaaaacaagg cccactcaat
    11761 aagatgatct gttactgttc ctctttttaa atgacggatt gccatgatct ggtcaattgt
    11821 ctgaccattc ataaacatct gcttggtgat catcatgctt tgattgaaat tttgctgtaa
    11881 taatgaatcg atcaaagcgt gccaaagcga atctggccgt ttttcgattt gtgctaacaa
    11941 aaggtggata catctgcttt gttgcagata aaccgctgac tcatcattgt tttttgccag
    12001 ctgataagga agcagccctg ttcgctcatt tccagataat tggttagcta aaaaatctgc
    12061 tgcttcttct ggtatctccg aaaacagctg caccaaactc tcatacgcag tatcaatcaa
    12121 aatttcacgt ggcaattggc tttcactaag ccattttttt agttggaaag tataaaaagg
    12181 acttgtctct gctggaagat agtcttgaat acccgttgcc agattagaaa ttacttgaac
    12241 agcaaattta atgagccgcc aactagtttc ccctgttctg ccatagcggt caaaacgcaa
    12301 accagtatgt tctatttgat gttcggacaa catatcagcc cctgcaggcg ttagctttgc
    12361 ttcattttca tttatttcga tccaaccttg ctgcgcaatt ttttgcaaaa tttgataaaa
    12421 cttatcctgc ttcaattctg gaaaactgtt atggataaac aaaagttcgt gggtaaatcc
    12481 ataaataagt actgaagtcg ttcgttttcc cacaaggagt tggtaaagag aactaatccg
    12541 cagcttgtgt cctgatgaaa atagcgataa aataaaaaca atcatattat tctccttaag
    12601 ttaggatggt tagaaatgtc actactatgt aaacttgtac ctgaacgctg tattgcatgc
    12661 ggtctttgtc aaatcgaagc gccagatatt ttcgattatg atgaagaagg aatcgtttta
    12721 tttgcccaag aaccagaggc tcatcaacag ttcgtgatgc cggaagaaga aacatatgta
    12781 aaaaaagcgt atcaaaaatg tccagttcga gcgattttat tagaaaataa aatataaata
    12841 taaacgaaac tttaaaaacg tacactaagt tctctaaact tgaaaataag ccgggatctg
    12901 tttctcttgt tccgtgaaat cccgacttat tttgaaagat tcatttgcta tctatagtta
    12961 attgactagt tttttcacgg accctatagg acccatttat attatttcaa ctttttcagc
    13021 cagttttcca atgtattgac gatcacagtt tgctggtctg cgttagaaat ttctgcctct
    13081 ccatctcctt tttgctcacc atagctgcca aaacctccat gatttccgcc attgattgat
    13141 acatactctg ttctttccgg taaatactgc ttagcttctt gataagcttc ctgattcaat
    13201 actttatctt ttgtcgccgt gatcgataaa acaggaacag ctgattctgt caatgttcct
    13261 tttttgtcgg gataacttgc catgaagaat acccctttta aatgtttgtc tgaatgattc
    13321 ttattgacat aacgacttgc cattacgcca cctaatgaat ggccaccgat cacataatcg
    13381 gaatttggaa aatcttcttt gaccttatcc gctttgtttc cgcccatcac tgctagatca
    13441 agtggcattt tgacaatagc gactgaaaag ccgttttttg ctaaatcttg cgcccaaata
    13501 ctataacttt ctggctctac gagagcacca ggataaaaaa taagtaatgg agactttcca
    13561 tgattctttt tctctccttc aaaaaataag taatcttctt tgttttttcc ttcttttacc
    13621 aacacttgcg cttctgacga tggttcgtac gtattttgct gtatatagta ataaccggct
    13681 ccaccgagta atcctaaaat gatgactaag ccaattagta ttcgtctaaa tattttcact
    13741 ttctcccacc ttttctatct gagtgacccc tttttcttaa tcaagtatga ttatacctta
    13801 aaaaaataag agagaaatgc aaactttcca gcatcaaata aaaaaagacg gaatatccat
    13861 ttgaatatcc gccctttttg tatgtttatt tttatttagc acataaacga tacacagctt
    13921 ctgcataaat ttccattgct ttatacatac tacttaattc ccatcgttca ttcgcctggt
    13981 gcataaagtc aggagtatca gggaacatcg caccgaatgc cacacactgg ttcatcgtac
    14041 gagcaaaagt agcgccgcct gatacaaacg gttcagtcat gtctccagta atatcgcgat
    14101 atgtccccaa tagatttttc actaactcac tgtctagtgg cacgtataat gaatcaagga
    14161 agtcaaattc ttcataagtc aacccatatt tttctgctgt tttagttaat ttagctacta
    14221 aatcatcttt cttgaatgta acagggattc tcatatctac accgattttt gtttgttctg
    14281 gtgtgatttc caaacttgca aagttcatcg ttagctcgcc ggattgttca tcttctgttt
    14341 tgcccacgac agcttcacca gttgcatttt cttgaactaa ttttcctaag aaattgattg
    14401 ggccaaaatc aaatacttct gaaagagcaa ttgccagacg agaaatagca ttcactcctt
    14461 gtgctgcatc tttcgcgtgg atacttttac caagaacgac gatgccttca ccttgttctt
    14521 cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc agaaagcttt tctccagagt
    14581 aaggagctgc atcaggaaca acattcaacg ctcctccagc ttttacagaa aactcgttcg
    14641 ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc tgcataaatt aaaggaaatt
    14701 cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt ttcgttatat ttttctaaac
    14761 aacgccataa attttcttca tctgtaccaa agataaaacg aatacgtgtc ttgaattcca
    14821 cgccagcatc aatcaaagct ttgacggcat acattgccgc aatagaagga cctttgtcat
    14881 cttgggatcc gcgaccaact aaccagccgt cttttatagt tggatcgaat ggttttgttt
    14941 cccaattatt ttcgtcccct gctggaacaa cgtccatatg acacaagata ccgaataatt
    15001 catctccaga gccaatctct gaataaccgt aatagccttc tggatcttta aatgttctaa
    15061 atcctagctt ttcactgatt tccaatactt tatccaaagc ttcaatcact tttttaccga
    15121 agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga aggaattcgg atcaattcgc
    15181 tcaatgattc taatgcttgt tcatgatgtt gttctgtcac aaattttttc atgatattat
    15241 cctcctaata tgttaaaaag ggtcatgtca aattcctccg acacaacctc ttcttctcaa
    15301 ataatgacgt tttaagcctg ctccatcagc aagcagaatg ttcttaacaa atgatgaaag
    15361 aaaaaacact cactgaatgg agctattgac ttatccacgt gcttatcgat agccgtttat
    15421 cggtttcttc attcttatga tagaccttgt acctgtattt gtctaggttt agaacattgc
    15481 tgctacacct aggatcacac agatggctac aaataatatc gcaattaatt taccggtaaa
    15541 tttccaccac actgaaacat cgaaacgagc aattgctaca gcacccatta cgatagcaga
    15601 tgttggtgta attaagttta ctaaaccaga tgcagattga taagcagtga ttactaaatc
    15661 cttaccgacg ccggcaaatt cacccatagg tcccatgatt cccattgttg cagcagctaa
    15721 gccggaagtt gaaggaatca agaatgacat agggatatag aaaataaatg taataatgat
    15781 gaatacactt gaagataatg aacttaatcc ttgttcgccc caatgtaaga tggtgtctgt
    15841 gattaatcca ttgttcataa ccacttggat accacgagca acagctacta cgattgctac
    15901 accgatcaaa tcagaagcac cagctaaaaa ttcagagaca aattcagact ctttcatgcc
    15961 ataagtgatt gctactacga tggacattac taagaataac atagtaattt ctgggaagta
    16021 ccagtcacct aatggaagca tgcttttacc tagaaccgta cctagtaccg gaaggtttgt
    16081 taaccagttt gtcaatgatt caaaaatcgt gaaattcgca ttcaaggttg tccaaggaat
    16141 caaactgatg atcatgatac caaaagtaat aaagaacaat acattaacat gtttttgttt
    16201 tttcgtgatt acttcttgac gaccgattgc ttcaatatcg aaatgcttca tatcttcttt
    16261 acgttggttg tatactaatg attttgttgg atctttttcg atctttgatg cataacgata
    16321 cacatataca atagaaatag ctaacaaaat aacgaacatc aagaatcgaa gaccaatacc
    16381 ttcacccatc gaaatcccaa cagcttgtga agctacgcct gttgcaaaag gatttaccgt
    16441 tgaagctaga cagcctactt gagaccctac taatacgatt gctacagcta ctactgtatc
    16501 aaagcctaca gccatcatga ctgggatcag taaagcatag aatggaatcg tttcttctgc
    16561 cattccgtaa gtagagccgc ctaatgcaaa taacagcatc aatactggaa taagcatttt
    16621 ttcttttcct ttgaatttct tcacaactga agcaatcccg gcatctagtg cccctgtttt
    16681 gttgataata cctaaaaatc caccaacaac caaaatgaag aaagagattg ggattgcagc
    16741 aggtgtttct ttagttagct cccccgctgg tacacctagc atcccattga ttggcgccat
    16801 gaaaatatcc cacagccctt gaggattttg ttgtactcgt tcatatgatc cagcgacgat
    16861 gttgcctgca tcgtctacac tatagtgccc agctggtata aaccaagtaa gtacagcgat
    16921 tactgcaata ataataaata agaccgtata cgctgaaggc atttgaaatt ttttcttttt
    16981 tgtttgttcc atcttcttct tcctttcttt tccgttagag acaacaattt tggctctaat
    17041 gttattttat caaaaagagt atcagaacaa aaataataac acgcattttg taaaactttt
    17101 gttcaaaaag ttcgtttttc cctattccct cctctttttt caggttttta aagctttctt
    17161 ctttttcatt gtagcgtttc ctttttttta taaacaaaca ttttcgtctt ttaaaaggag
    17221 aagaatttca catttgcaaa gaattgattg atttttatgc ataaatcaaa aaaatactca
    17281 aaacaaacaa ataaaacgtt tacattattg catgtcttgt atttttttcc aaaaaaaaag
    17341 accaagaaat ttcttgatct ttttgatatt tatttgactg ctgtatgacg ttgttcgaac
    17401 tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat gcagcactaa aaagacagca
    17461 ctcacgatga atcctttgat caagttgaaa ggaacgatcc caatcagtac atagtttgct
    17521 aaagacatgc ccagcatctg tgttgcgttc agccctaaaa acattaagta aagaggcgta
    17581 ataacgaaat aatttgcgat actcataaaa atagttagag cgatggttcc tgtaatgaca
    17641 cctaaaaatt tgtttcttcc tttatgctgt ttctttacac taaagaaata ataaatcggg
    17701 aatgtaaaga tcgttgttga taagaaacta gcagtgtctc ctaccaaatt atccggagaa
    17761 aaacctgtag tcagcagatg taatactgaa cgcaagaacg ctgttgcaac tccggccgct
    17821 ggaccgaata agaacatgct gatcaatacc gagatatcac taaaatcgat cttcaaaaaa
    17881 ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa ctaatgcgat cgcagcaaac
    17941 atcgctactg caaccatttt ctgtacacgg gtgttcttca tttgaaatcc tcctgttagg
    18001 actcctcttc agcgaacttt ttgacgtttc tctccaaatg aaaaaactct attttccagg
    18061 gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat aagctctatc ttctttatcc
    18121 agactatact gtcggtatcg gaatttcacc gattcagcta tttttttgca aaataggtcg
    18181 tggactataa ccaccggtcg ggaatttcac cctgcccctg aagacgaacc ttatataatt
    18241 tttgtttaca tattcattat acatatctat tcaaaaaacg caacatctaa cacttataaa
    18301 aagtaagtgt tcattttgag tggttcagca aactgctgac ttcttttttg ttcagttcac
    18361 ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc gtatttttca cgttttagtt
    18421 tttgcacagg aagaccaact gcttgaagca tatttttcac ttgatgatta cgtccttcgt
    18481 ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc cactgataaa atttggaatt
    18541 tagctggaga tgtttttcgt ccttcgatct ttattccttt tgtcaaaggt gcaagcatcg
    18601 attttgtagc gatccctttt acctttgcaa cgtaaacttt atccacttca tgttttggat
    18661 gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag caagatcccc gaagtatcgt
    18721 aatccagtcg acctacagga tagatacgtt cttttaccat cggcaaaaga tccgtaacaa
    18781 cttttcttcc tttgtcatct gcaacagcag aaataactcc tttaggtttg tacaataaat
    18841 agtaaccgta ttcttcttga taaattggga cttcatccac ttccacagtg tcatgtttcc
    18901 caacttttat ccctaattcc cttacaactt gtccgtttac ttttacacgt cctgcaagga
    18961 tgaattcttc tgcttttcga cgagaagtga tgccagcgtg ggcaattact ttctgcaatc
    19021 tctccattac aattattctc ctttattcat tccatcaaag aatagatctg gtgacacgtc
    19081 ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt tcttctaatg atttcaaacc
    19141 aaaataatct aagaagtagc cagttgttcc atataatatc ggccgacctg gtccttctac
    19201 tctacctttt tcttcaatca gtttatgggc agctagacgc tgtaccgctc ctgaaacctg
    19261 aactcctcga atctgctcga tttcagctct tgtgattggc tgtttataag cgatgattgc
    19321 taaggtttcg acggcggctt gagataatgt gtttgatgtc ccttgcgcat atcgcttaag
    19381 taagggagcc agctcttttt tagtcgttaa tacataatgg ctttccgttt caaaaatatg
    19441 caaagctgag tgcgagtttt gagtgtaaga ttcttttaat tctttcacta aacttgtgac
    19501 ttctgcttcg ctgagattta acagtccgct taattcttca ttactgatcc cttcctcgcc
    19561 aacaataaaa agaagggctt caagttcact taatttcgtc atgttcggta tctcctactg
    19621 ctggataaag cataattgat tcatagtttt ctttttgttg cgcaatgatc tgcccttttt
    19681 tcatcaattc tagcaaagcc ataaaagtag tgacaacttc acttttagaa ggtgactcaa
    19741 aaaaagaatc gaaagtacag ccatgcgcat ttctttttat acgctcttcg ataaatacca
    19801 ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt ttccactttt tgagaaaatt
    19861 tcttacgttc taagattttc tgcatggcac ggatcaaatc gatttttttg acttgtcctc
    19921 gcttcaacgg cttttcagca tccgctaacc catcgatgtt cataggttct tttgtgtaat
    19981 aaagacttcg ttcctctgct ttttctgtca gtctttcagc tgcatattta tatttacggt
    20041 attctaaaag ctgagtgacc aattcttctc gaggatcttc ttcccataat tcttcatcgt
    20101 ctgtgacgaa ttcttggaca ggaagcaatg tcttgctttt gattgccatc aatgtagcag
    20161 ccatcactaa gtattctcct gcaacagcta gctcgagtgt tttcattgta tggatataat
    20221 tcatatattg ctccgtgatc gtagcgatcg gtatatcata tatatcgatt tccatttgct
    20281 gaatcagatg aagcagcaga tcgagaggac cctcgaatac atctaattta atattgatct
    20341 ccgtcaactc attcactctt tcttcttttt ctttgctagc cattttccaa tgtaaggctc
    20401 tgtgatttca gttgcacgga tggagacatc cgggtacttt tcctgtaatt catctaacat
    20461 ttgaaagccc atattctcac cgcgataaga cggattcaaa gaaatgtcat ggatgatcag
    20521 ctgatcgttc gttgcccagt aaataccaat aatcccttga atgttggttg aaccttctgg
    20581 cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac gtatcaatct ccttcaacaa
    20641 agaagtttta cctgtcagtt tcttatggaa actcaatagt cccatagcaa ttttacggtt
    20701 ttctttccgg taatgtgtta acatcttctc accttcctat gactaagccc ttggaaaatg
    20761 ctgcttataa acatccgtca ttcttttctt cgtgatatgt gtataaatct gagtcgtaga
    20821 gatatccgcg tgacctaaca attcctgtac tgtccgcaaa tcagctccat tttctagcag
    20881 atgtgtggca aagctatgcc gcagcgtatg tggtgttact tctttgtaaa tcccagcctc
    20941 tcgtactaac tgcttcagat ttttccagat tccttgccga gaaagacctt tcccatgatt
    21001 attgacaaat acatgcattt cagatgcgtc ctttacaagt aatggacgtg cttcgtctaa
    21061 gtagcgttcc agccattgga tcgcataatc acctaaagga atgatccgct ctttatctcc
    21121 ttttccaact gtctgtacca gtccaagaga aagatgaaga tctcccaatt tcaatccaat
    21181 caattcactg acgcgcatcc cagtagcata catgacttcc aagattgcac gatctcttat
    21241 ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt tctacttctg tcagagtcaa
    21301 tgtacttggt aatttctgtg ctttcttcgg cgtatcgata tgttgcatcg gatcatgatc
    21361 agtgatacgc tcttgccgta aaaactgatg gaatcttctg agacttgaga tcatccgagt
    21421 aacagttgct gtcgcttttt tttcttcatg caacttctcc agaaaacgga tgactaaaaa
    21481 gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt agaaaatgca agtactgttc
    21541 caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat ccccgttcaa tattcaagta
    21601 gtggagataa tcgttgattt gctctttcat aataatcccc ctcatgcctc tctatcatat
    21661 cagataccgc ttcattctga tagtcttttt ccataatctg agcaaagaaa aagccgtcta
    21721 gacactcaga cggactggtt t
//
LOCUS       DACCQI010000050        21266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCQI010000050 DACCQI010000000
VERSION     DACCQI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.050"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(37..1839)
                     /locus_tag="IVO76_001430"
     CDS             complement(37..1839)
                     /locus_tag="IVO76_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429400.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(1853..2605)
                     /locus_tag="IVO76_001431"
                     /pseudo
     CDS             complement(1853..2605)
                     /locus_tag="IVO76_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(2614..2976)
                     /locus_tag="IVO76_001432"
     CDS             complement(2614..2976)
                     /locus_tag="IVO76_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7429401.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(3152..4024)
                     /locus_tag="IVO76_001433"
     CDS             complement(3152..4024)
                     /locus_tag="IVO76_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7429402.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(4050..4562)
                     /locus_tag="IVO76_001434"
     CDS             complement(4050..4562)
                     /locus_tag="IVO76_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7429403.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(4700..5530)
                     /locus_tag="IVO76_001435"
     CDS             complement(4700..5530)
                     /locus_tag="IVO76_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7429404.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(5546..6154)
                     /locus_tag="IVO76_001436"
     CDS             complement(5546..6154)
                     /locus_tag="IVO76_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7429405.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(6147..6464)
                     /locus_tag="IVO76_001437"
     CDS             complement(6147..6464)
                     /locus_tag="IVO76_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7429406.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(6620..7348)
                     /locus_tag="IVO76_001438"
     CDS             complement(6620..7348)
                     /locus_tag="IVO76_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7429407.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            7507..8388
                     /locus_tag="IVO76_001439"
     CDS             7507..8388
                     /locus_tag="IVO76_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7429408.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(8625..9668)
                     /locus_tag="IVO76_001440"
     CDS             complement(8625..9668)
                     /locus_tag="IVO76_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7429409.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(9671..10339)
                     /locus_tag="IVO76_001441"
     CDS             complement(9671..10339)
                     /locus_tag="IVO76_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7429410.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            10481..12817
                     /locus_tag="IVO76_001442"
     CDS             10481..12817
                     /locus_tag="IVO76_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7429411.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(12932..13243)
                     /locus_tag="IVO76_001443"
     CDS             complement(12932..13243)
                     /locus_tag="IVO76_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429412.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            13424..14899
                     /locus_tag="IVO76_001444"
     CDS             13424..14899
                     /locus_tag="IVO76_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7429413.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            14946..15530
                     /locus_tag="IVO76_001445"
     CDS             14946..15530
                     /locus_tag="IVO76_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7429414.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            15532..15951
                     /locus_tag="IVO76_001446"
     CDS             15532..15951
                     /locus_tag="IVO76_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7429415.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(16232..16516)
                     /locus_tag="IVO76_001447"
     CDS             complement(16232..16516)
                     /locus_tag="IVO76_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7429416.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(16869..17579)
                     /locus_tag="IVO76_001448"
     CDS             complement(16869..17579)
                     /locus_tag="IVO76_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7429417.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(17576..19048)
                     /locus_tag="IVO76_001449"
     CDS             complement(17576..19048)
                     /locus_tag="IVO76_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7429418.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            19186..19659
                     /locus_tag="IVO76_001450"
     CDS             19186..19659
                     /locus_tag="IVO76_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7429419.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(19812..20363)
                     /locus_tag="IVO76_001451"
     CDS             complement(19812..20363)
                     /locus_tag="IVO76_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7429420.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            20468..20998
                     /locus_tag="IVO76_001452"
     CDS             20468..20998
                     /locus_tag="IVO76_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7429421.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
BASE COUNT         6574 a         4315 c         3733 g         6644 t
ORIGIN      
        1 ttggctgttc ttattacttg atgcaggaca gctttttcac aatctctttt tcaatacgat
       61 tccagtatat aaccatgtcg tcagtacata gtagatgaaa tacaatacga agaagatcgt
      121 aaacggaacc caaatatctt tgtatggatt ttgcatgaat agaccaaaca tttgtaatcc
      181 gaataagaca tggactacac ctaagattcc tggtgctaag aataacacgc ctacttctct
      241 acggatcgat tgtttaagca acccgcgtcg tgttcctatc ttttcaagca tttcataacg
      301 agcaatgtca ctcttgcttc ctgacaagat cttgaacatc aaacagctag caagcatggt
      361 caagaaagct aaacctagga agaatcccat aaattcaaaa ccagagaata gaccattata
      421 cacttcatac atttcatatt tctggttgct gtactctact ttttgcaaag atggattgtt
      481 cattttattt tgagcaacaa gtgtttcgat tggtttcagg tttgcttgga agtcttttac
      541 ctgaactagc tgtaaagtgg attgtggcaa gttcagttga tcaaattctg attgactgac
      601 taccttgatt tctttttctt gctgttccgg caattcatag cttcgaagtt cctcttgtac
      661 agtcagttca ttcatttttt cagcaggaac tttttctttc gtgatcttcg gcggtgttcc
      721 atcttcatgt tttatcatga tcaatggttg agccgcaaat tcacttgcat tatagtaaat
      781 cgttcccgca tcttcttttt gagtataaga agcagaaagt gttggtgaaa gttctttgat
      841 tttttgctga tcttctgccc gaggattatt catcactaga tcatatccag aattgttttc
      901 tgtcaagtct gcaatttcat ttttaaaacc taatcctacc gtcaaagcgc ccagtgccaa
      961 ggcaaacagc atagcgacca ttgagagcat ttgtgtatat tcacgaatac gaaagtttaa
     1021 ctgtgacaat gtgaaattgt tcaatttttt caatgaaatc tgttctgatt tctttagtaa
     1081 tgataaaaag aaaatgatca cagaatggaa aatgaaataa gttcctaata cgatcgtaac
     1141 taaggctact cctataccaa agacttgata ggtccccacg tgtttcatca tatagtaacc
     1201 gcagcctaga aaagcaattc ccagtactac ttcaaggaaa aacaagaatt tatgctgctt
     1261 gattcttgtt ggtgtctgat ccgcacgaat caatgctaat atcggttttt tgacaatcga
     1321 aaaagcattg acaacagcag ctaataaaaa caaaacagca aaaaacaaaa cagtgatcaa
     1381 caacccgtga atactaaaag gtgtaaaatg cgaaatattg agatctaact gatccactaa
     1441 taatcggtgg acgatacttg tcaatcctac accgacagct gaacccacta aagtagctgc
     1501 gatccctact aagaatgttt cagaaaaaat catctgtgcg atttttctgc cttttgctcc
     1561 tagcatcata aacattgcat agtctttttg ccgcatcgtc atcaaaaagg aatttgcata
     1621 taaaatataa acgaatgtga tgatccctag taaaacagaa ccgaatcgga aaatgataac
     1681 tgtagccgca acgatcgtat tacttttcaa aaaagcttcg ttgcttgcca ttgattcaaa
     1741 catgtaaaaa atagcagatg ccataactaa tcctgagaat aacaccaggt agtctcttaa
     1801 acggctcttg atcccagtaa gtgataattt ccaaagcata gtcaatctcc ccctactgtt
     1861 ccaagtttcc aagaatagat agtatttctc gatagaagga atcacggctt tgatctccac
     1921 gtttcacttc ttgatggata ccgccatcct tgatgaataa aatacgttgg caataacttg
     1981 cagaaaacgg atcatgcgtc acaagaagaa tcgatacatg atcttttgta ttcaattcgt
     2041 ccatcgtatc taaaagatcc cgggcacttt tggaatccag cgcacctgta ggctcatccc
     2101 ctaatagaat cgttggttgt gtgatcaatg cacgtgcggc ggcgacccgt tgtttttgtc
     2161 caccagaaat ctcagaagga tagctttcca aaatatgaga gatggataaa cgttctgcaa
     2221 ttttttggac acgttgcttg atttcttttg gtctgacgcc ttgtaaagaa agaggaacag
     2281 cgatattttc ttgtgctgtc aagctttcta ataaattgaa gtcttggaaa ataaagccaa
     2341 tctcattcgc acgaaaatct gctagttgat ttccttttaa tgtggtcaca tctttttgat
     2401 tgattcggat atgcccatct gttggtttat ctaatgtgga taaaatattc aaaagtgtcg
     2461 atttaccaga accagaagcg cccatgatcc caataaattc gccttattct acatcgaagc
     2521 tgatgccgct taatgcttgc gtctttttct cattgttttt tccgtatact tttgttacat
     2581 tctgtacttc gactattttg ttcatttctt tccctaccct ttaattagtt tttccagtgc
     2641 tgttttcgga acgtcttttt tttcgacttc ttcatacgaa gtgacacctt tatttttgtt
     2701 ccaggtgatt tttaaatatg ctcctttacg aagcggtcga gtcaattgtc ccttgaagtc
     2761 cagttctttt tccttaccct ctttcgtata gccaggcagg atatatctat agttgactgt
     2821 ttcttgtcca gagtctgatt ttgaaactgt ttttgtacca tctgtcgtaa tttgtacata
     2881 gtattcttct ccacccataa ctacgtaatc tgcgattttc acccctgcaa gtaccacaac
     2941 gatgaaaaca agtagaccga tcagaagttt tttcactttt tccccttctc tcttacttta
     3001 tatactgatt atagaaaaaa gcgaacaatt ctgccttcga atcaactatc attcttcttt
     3061 caattttgtc attttcttta aaacaactaa aaaaagagtt caagacaata atccgtccta
     3121 aactccttct tcctatctta caaaaagtat gtcacttacg gtctttcatg tcgcctgtat
     3181 tgataacagg ttgcgttcct ttgtctgtaa tgattgagac aagtaaatta ttgatcaatt
     3241 ggactttccg atcatcggtg aaattgattt cttgtccatc ttcgatttgc tctaaggcca
     3301 tttgagtgat tgtgacagct ccttcaacaa tggtttgtcg agcagacaaa atggcttttg
     3361 cttgttgacg ttggagcatg gcactagcga tttctgttgc ataggctaaa tggttcaagc
     3421 gtgtttcgat tacttcgaca cctgctactg ctaaacgctc ttgcagctct ttttgcaact
     3481 cgtcagacac tgccgtcgtg tttcctcgaa gcgtcaaatc attctcattg aatgtatcat
     3541 acgggtattg gctagcgata tgccgaatgg ctgtctcact ttggatttct acaaaatctt
     3601 gataatacgc tacatcgaat aatgcctttg ctgtatcaac gactttaaag actactactg
     3661 ctgagatttc gatggggttt ccatccaaat cattgacttt taaaacagaa ctattgaaat
     3721 tccgaacttt caacgaaaca gtcatttttt gtgccaaagg tatcgtaagg aaaaagccat
     3781 tttctcgaat cgtacctaaa tattgtccaa aaaacaaaat tacttttgct tgattaggac
     3841 tgactacagt tgccgaactt aacaacaata acgtgatcaa ccaaagaaaa atacttaaaa
     3901 aaagtgccca aactttcgct tgccacatgc ctaaatagaa gaaaaacaat ccaactaaag
     3961 ctaatacgac caatccgaca atccctgcat aaccattgat ataaaatgct cttttttctc
     4021 tcatgctact tcctgcgctc ctttatcctt caataaatat ctaatgttac ttttttaatt
     4081 ttttttcgaa tgcgcgccat tacaccttct agttctaact ctttgtcttc agtgatgtac
     4141 caaccgcttg cctgtgtccc tccggctgcc tgcggtaaat aatccaattt tgttcgaacc
     4201 cagtgctttt cttctgtaaa atagtcttct accaagacct ctagatccat ccctgctgaa
     4261 agaggcaaat cttcccgaac agatttaaag ataaatcgtt ccttcttccc atcatacttc
     4321 aatattcctg tatcgataat cggtaaagaa taataatcca gctctttctt caccgtcaat
     4381 aaatgtttga aaacttcatg gtacacttgc tctaattggc gaccttctga attgttcggg
     4441 taattaacca tcactttttc tttgtctgga tcatagaagg tttggcttag ttgagtgata
     4501 taagtatgta atctttccat ttctgtcttt aacttttctc tttgaccttc ttctaccttc
     4561 atatactctt tcaccctgtt ttctttttag atattttctt tcttatttta ctatagttat
     4621 gtgcgttaag cgatgaaaaa cagcaaagat tggaaggatc tttgctgttt tttcttaaaa
     4681 tatccagttg agtacaggat caatctgttt cattgtcctc ttgatccgtc gtaatattga
     4741 tcgatgtgtc tttagcagtg atctttagct ttttctgtcc ttgttctaac aaagtatttg
     4801 ttattggctg gtagtctttt tcaatataag attcaccaga cgtttggata gtatagccac
     4861 tgttttccat ctcatgcaaa gataaattac caccgtttag gatgactttt ccttctttgc
     4921 tgatcaaact gtcttctaga tggaagttgc tgttttcacc tgtgacgatc aagttatcca
     4981 cattggcctc atctagtcga agtgagccta attcatcttt cgcagttatt gttcctcctg
     5041 taaattcatt gaacgatacc cagccatcat gtgtcttagt attcactttt ttgattgata
     5101 cttcgttgat atcaattgca ctattattcc cttcgattgt caattcagat aattcatgat
     5161 ctgcagggac aacaatagtg atttgtgcac ttgtgtcttc taaactaatg attgcacctg
     5221 ttgatttttt gactgcattc acagtcaaca cgccattttt ctcctttatt tctggctttt
     5281 ctgtcttatc ataagtcgtt tttatctcaa aatccaatcc gcgtatgacc gaaactgttt
     5341 gattttctgc atggatgacg attttgtttg catccttgat tttttttgtc tcagtaacca
     5401 tatcgatcaa ccgaggacca ttatcccaag tcagactctt ttgagcgcct aatgtataag
     5461 ttgcacctgc taggaggcca ccagtaagca tcagaccaat accggtaatc aaaaaatgtt
     5521 tcaatctcaa tttatgtgtc ctccttcaaa cgcccgctga atgcagatct ctttttgtaa
     5581 taaaacgtcg tcccatccat ttaacaaagt tcatcatgac ataaaaaagc ccttttgttc
     5641 ctttcagcaa cagtggcgaa ataaatactg cgcttcctgt ggcgaacaga ccgacaccga
     5701 taaagaataa tccactcaga atcgattgtc caaacacact gattcctgag atgatcatga
     5761 ataggccaac acctaagata ctgatacata aaacatagat actaaaaatg agcgctagta
     5821 aagcaaccaa tagtccccca ataaccaata taaaccccag tgctagtgga atgaggacag
     5881 gagaagcaaa taaaccgata atgattaacc aaatcaggcg aatttgtctt tttggatttt
     5941 tcactaactc gttttctgct tgttcttctt ctgaaaagta actgattttt aattttaagg
     6001 caaactgctt tggtgtacca tattcatcaa tgatctgatc taatgttttt ccagaatcta
     6061 aaaaatattc ctcataatac tggatcaaat caaaacgatc gtcttcagga atttcttgaa
     6121 gatgatccac aacttcattc aaatattcat tcatttgtgc tctcctccaa taaccggtct
     6181 aaagaatctc gaaaaacaat ccattcatca ataatttctt ctaaatgacg acttcccatt
     6241 ggtgtcaacg agtaatatcg acggtttctg ccttcaaaag gttcatcata ggtctcaaga
     6301 tatccttctt ttttcaacct tctcaaaacc ggatatagtg tagattcact gacagagaga
     6361 ctttcttgta cgatttttgt taaggcatac ccatacatat cttccttgct taatgctgct
     6421 aacacagtac catcaagaag aacagttgga atctggatgt gcattacgtc accttctatt
     6481 ctcaagtgta atattttatt tcaatctata ttatacaaca tataatatag tttcaaaatc
     6541 agtttgtcaa atacaaaaaa actgactaaa agatttgatc ctttttagtc agcaaaaaat
     6601 ggcttctctt tgatttttat tataacagtg gcataatcca atttgtccac atccagctca
     6661 gtggcatgat aacgatcatt gcaggtatca tgtcagctac tgggaacatt ttcacgttga
     6721 tcattctaaa accggtagcg accatcaaga atccgccgca agctttaaaa tctaagatca
     6781 tgtctggtgt agtcaatgga taaataaatt ttgccaataa gaacaaaata taaaaaataa
     6841 tgaattgggg aattgctatc acagaaacga cataccctaa attacacgca aaaatagctg
     6901 ctgtgaaaaa atcaaggaca gatttagaaa tcaaaatcgt actgtctcct gtcatcccag
     6961 aatctaaact tccatagatc cccgtaccac ttgcacaaaa tagaacaatg accgtcacta
     7021 atgtgctgat aaattcatcg tgggtcatac caagcttttc actagggaag attttagaaa
     7081 taggtacttg catcagcgca gcccccttat ttatcccatt acctaaatga acaatcaaac
     7141 caatcgctgt tcctatcact aaagcaaaaa taacagccgg catatttttc atcggcgcaa
     7201 tggaatagat tcccatcccc atcgaacaca caccaaaaac catcgtcagc tgtgctttaa
     7261 aatgttcact taatttattt cctactaatc cgccaattag tccgccaaac aaaacggaac
     7321 ctacattgat caaaactcca gttggcatca aaaaactcct ctctttttct acgaagcgaa
     7381 aaaaatcaga aaaatttctg taattattgc tttcgctgca agaaagattg taacagacag
     7441 agaagattac accaattcat tttttgtgtt aaagtattct ttattggaat aagtaggagg
     7501 aatatgatgg atatccataa attcgaagtt tttcttgatt tagccgaaac aatgaatttc
     7561 accaaaactg ctgatcgaca attcaccacc caaggaaata tttctaaaca gattctttcg
     7621 ttagaaacag aattagacgt caaattattc gaacgcgccc atagaaaaat cgaattgacc
     7681 gaagctggaa gcttattgct cccatatgta aaaaatgtcg tagaacaata ccatgctatg
     7741 caagaagtgc ttgaaaccta tacgaaagac aacaatcttt cattaaatat cttaacgata
     7801 ccgacaatga tcaattataa aggtttttcc aaaatcaccg agtttttgaa acgccatcct
     7861 gaatttacag tccagttgaa agaagtggaa agtgtcgaat taacttacaa tgacgccaat
     7921 gaacatagtg acacgatcca ctttgctaga agtttccagg ccccaccaag ttcgattgag
     7981 tggttgccta ctgaaacaga cgattttgta gcagttctac ctaaaaatca tcctttagct
     8041 agtaaaaaga aattggatct aagcgaactg aaaaaggaga actttttgct ccttggcccc
     8101 actacgaact tgtatcaacc tgtactagat ctgtgtcgcg aagctggatt tgaaccaaaa
     8161 atctcttata aaggagcaag aatcgacttg atcatcaata tgattgcaaa tgacatgggg
     8221 attgctattg ttatggaaaa aacagtcaag aatctattaa aagaaaatac tgtgattctc
     8281 cctatttctc caacaacaga gagttatctt gcatttactc gtaaagtcgg tgagcattct
     8341 ttggcttcag atacattttg ggagtattta aatcaagaag aaaactaagc cctacatttt
     8401 ctgtccatct atccacccaa aaaaagagag cgacgcatga ctttcgctct ctttcttgca
     8461 tcatgtcctt gtttcaatga cacctctaac atttctcgta gttcaataag aaagcagcga
     8521 tttttatgcc aaaagaaccg taagaacatt tccctaatca caaaaaaacg actaaaaacc
     8581 cctaaaaacc ttcaatagaa tttttagggg ttttattctt tttcctattg tttacttaac
     8641 cgtatcattg ctgtcgttcc tactccttcg atactgctat aatcaatacg tccatcatgc
     8701 agttctagaa tttttttaac cagtgaaagt cctagcccat tcccagaaat ttgacgactt
     8761 gtatctcctt gataaaattt ttcaaacatt cgatctctgg tctcttcatt cattcctaca
     8821 cctgaatcgg tcacttccag tacaatctct gtggctgtct caaatagttt gatatgaatc
     8881 tgtccattga tttgattgta ctttatggcg ttatccatga tattcagcca cacttggtac
     8941 aaaaactctt cattgcctgt gtaatttact gcagcaagtt cgatatccaa ttctagctgt
     9001 tctttctccc atttaggctg aaaaaaaacg attacttctc tgatttgttc atctaatcga
     9061 tactctttct tttccagttg gatacgctgg ttttccagtt tgttcaattt taagatgttc
     9121 tctgttaatt gtgaaagctg cgtgatactt tcgataatcc gatataaaaa tatttggcgt
     9181 tcttcgtcag agagatttgg ggcttgtaac aattgaacat atccttggat tgttgctagt
     9241 ggtgttttga attcatggga tacactggac acaaagtcat tcctcagtgt ctcgatacta
     9301 ttgagttctt ggaccataac gttgaaatct ttatacagtt gctgtacttc tgctactttc
     9361 tgctgttcat ccattcgaat cgaaaaatct cccgttgcta ccagactcat atttgtccct
     9421 agcgcactga ttggatgcag gatacgccgc ccaacaaaaa tcgatattcc tgtacccacg
     9481 accatactga accccgcaaa gacgaataac gggtataacg ttggttttcc agccgattca
     9541 gcaggcgaga tagaatgtcg agacaataag aaaataaaac cgagaaaaca cagcagtgtc
     9601 actaagatcg aaatgaagac aatactagca aaatagatcc ataattgtga aaatagcttt
     9661 tttttcatct ttaaataacc gccttatagc ctagtccccg tactgtgatg atttgaaaat
     9721 cttccgtctt ttcaaaacgg gtccttaaac gtttgatatg tacatcgatc gttcgttctt
     9781 ctgtattcgt atctaatccc caaatatcat ccatcaactg ttggcgagtg aagatcttgt
     9841 tgggataaga cagtaattta tacagaagca gaaattcttt ttgaggcaga atcaacgaat
     9901 gctgatcttg taacactgta taagcatcta gctctaattt tgtttgtccg attatcaagc
     9961 tactgctgta attgatcttt gaacgtcgaa gcaatgcttc cacacgtagg atcatctcat
    10021 tgacatcgat gggttttgtc atataatcat ctgctcctaa tttgaacccg cgtttcttat
    10081 cctcaaaatc ggcctttgcc gtaatcatta gaacaggtgt gtcaaatctg gtgctacgta
    10141 atgagtctag caagtcatag ccatccattt tcggcatcat gatatctgta atgattaatt
    10201 ctatacgtgt cgcttctagt tttttcagcg catcctctcc attcaatgcg gtgatgactg
    10261 aaaagcccgc tcgttccaac acagaacggt aaagcaactg taattgcgga tcatcttcta
    10321 ctactaaaat acgattcatt gatctccctc tgttttttga aaaatttcta gattttttct
    10381 tgtagttcat cttcagttaa catttatttc gtatactacg atcattgttc tgatatactt
    10441 attagtatac cgaatatttc aatacgggag aagggtgatt atgcttcaat taaaagatat
    10501 aaaaaaatat tataaagtcg gcgaaaccac caccaaagca ttggatggtg tgtctgtcgc
    10561 tttcagacaa aaagaattcg tagcgatttt aggtcctagt ggttctggta aaacgactat
    10621 gctgaatgtg attggtggac tagataatta tgattccggt gatatggtaa tcaacggaaa
    10681 gtctactaaa gactttaagg acagtgactg ggatgcctac cgaaacaact ctatcggttt
    10741 catttttcaa agttacaact tgattggtca tctaggcatt attgaaaacg ttgaacttgg
    10801 gatgacatta agtggtgttt ctaaagacga aaaacgaaaa cgggcagaag atgcattgca
    10861 ccgagtcggc ttgacaaatc atatgcataa aaagccaaat cagttatctg gtggacaaat
    10921 gcagcgagtg gccatcgctc gagcactagc caatgaccca gatatccttt tgtgcgatga
    10981 accaaccgga gcgttagata cagaaacaag tatccagatc atggaattga tccaagagtt
    11041 atcgaaagaa aaattagtga tcatggtcac acataatcct gaactggccc atcaatacgc
    11101 agatcgaatc attgaatttt ctgatggtaa gatcttaaca gattcccatc ctcacatcga
    11161 acgaccaaaa gatgatcaat ttaacttacg cagaacaaag atgagttttt ggacagcgtt
    11221 aaagctttcc ttcaacaata tccgaacaaa aaaaggacgt actttcttga cctcctttgc
    11281 ttccagtatt gggatcatcg gaatcgcgat cgttctgtcc ctttcttctg gattccaaaa
    11341 acaaatcgat aacacgcaag ctgaaacgat ggcgaaattt ccgattacta tttctaaggt
    11401 aacgactaac cagactcgtg acgatgccgg attgggtgca agcaaagcgg attatccaga
    11461 cagcaaaacg attactgcaa aagtcagcga agaagataaa gcacagcaca cgaataagat
    11521 tgatcaaaca tacgtcgatt acgtaacaga catcgatcct aacttaagta ataatatcgg
    11581 atttacccgt actacaggaa tcaatctttt acgtgatgta aatggcaaag tacagcctgt
    11641 cagcttctcg aatcaaaatc ctgatacgga atctctttct ttatctagta cgatgtcagc
    11701 gatgacaggt gtgggagtgt caagtttccc tacacagctt gacactagca aagaaaactt
    11761 tttaaaagac aattattctt tacttgccgg aagttatcca gcctcagcaa cagatgtcgt
    11821 gctgatcgta gacggcaata ataatactaa tatcaacgca ttgaagaatc ttggatttga
    11881 tgtgaaagaa gacgaaaaac ttgattttga tgatatcgtt ggtacaacat tcaaattagt
    11941 aaacaacaat acttactata caaaacttcc aactgggaat tttatcccta atacagatta
    12001 tgatgcgatg tatcagaatg cttctgatga attgaagatc tcggggatcc tacgcgtgaa
    12061 atcttcttct acaatgaatc tcttatctcc aggaatcgct tatagcgatc aattaacgac
    12121 tcaaatcgtc aacgaaaaca aagaatcaga aatcgtcaaa gctcaaagag acagtggtgt
    12181 aaatgtctta actactgaaa aagttgacga aaatgccaaa caaacattgc ttagttattt
    12241 aggtggagac agcttgcctt caagtatcat gatttatccg aacaattttg aagacaaaga
    12301 aaagatcttg gattatctag atgattacaa taaaggcaaa tcagatgaag ataaaatcat
    12361 ttatactgat ttagctggaa cgatgactga actgactggc ggattgatgg atgctatcac
    12421 ttatgtcctt attgcttttg cagggatctc tttagtaaca agtatgatca tgatcagtat
    12481 cattacttat acatctgtga ttgaacggac aaaagaaatc ggtgtactaa aagctttagg
    12541 tgcacgtaaa aaagacatca ctcgcgtatt tgacgcagaa acgtgtattt taggtatttc
    12601 ttctgggata ttaggtgtgt ttattgcatg gctagctacg ttccctatca atagcatctt
    12661 atacaacatg acggatctaa aaaatgttgc tcaactaaat cctgtccatg cgattattct
    12721 agtgatcgtc tctactatat tgacgatgct tggcggtcat cttcctgcac gaatggcagc
    12781 gaaaaaagac gccgctatcg ctttgcgtgc agaataaggt tgtgacaacc acatttagat
    12841 ttgagtcata agagcgctga aatagtagaa aacagcaaga aacgaggaag taatatgttc
    12901 cttgtttcgc gctgtttttt ttaattattt tttataattc tggtatactt ttctttcctt
    12961 tgtaccacaa ctcttctggc gtatacctgt cttttccatt tatgaatact gttccatctg
    13021 cttcaatcgt tacttcagtc ccaatccaca tattacgaga aagtgcgaga agattttttc
    13081 tttttcctct tccttttttt ccaaattgca aggcatcggt tatctcttct gtccaatgag
    13141 atttcacgta ttttacttct ccgttttcaa atgtaacttt caaaatattc gctatatttt
    13201 ccacttttac tgctttagga aatgactcct ccattttttt cactttcctt cctccaacac
    13261 taagtatgcg ctttctttta gtgataagta tatcatagtt tattctatta tcaaaaaaac
    13321 gcatccattc cattgtagaa tactttaccg aacaagttga attgttttcc tctttttcac
    13381 cttttactat agataggtaa agacaactaa ggaggaatct ctcatgacag atcaaccgta
    13441 tgatttaaga aaagtcatcg aagaactaaa aacacttcca ggtcaatatc acgaaacaga
    13501 tattgaaatc gatcctgaag cagaattatc tggtgtttac cgttatatcg gtgctggcgg
    13561 tacagtaaaa cgtccgactc aagaaggacc agctatgact ttcaacaata tcaaaggttt
    13621 ccccggcaca cgtgtgaaca tcggaacaat ggctagtcgt aaacgtgtag gtgaaatttt
    13681 gcaccatgat tatcgaacac ttggtcgttt cttgaaagac gctgtagaaa atccaatcaa
    13741 acctgtgaaa gtagacaaag cagacgctcc agcacaagaa gtcgttcatc tagctaccga
    13801 tgcagacttc gatattcgca acattttgcc tgcacctaca aatactcctg aagatgctgg
    13861 tccatacatc acaatgggtg tagtcttagg atcagatcca gaaaaaacaa tgagcgatgt
    13921 aacgatccat cgtatggttc tagaagataa agatacgatc ggtatgtaca tcatgcctgg
    13981 cggacgccac attggtcact tccaaaaaca attcgaagcc ttgaataaac caatgcctat
    14041 cactatcaat attggtttag atccagcaat tgccattggc acgactttcg aaccacctac
    14101 cactccactt ggctacaatg aactatggtg tgcaggagct ttgcgtaacg aacctattca
    14161 aatcgttgat ggtatagcag tcgatgaagt gggtattgct cgtgctgaat tcatcattga
    14221 agcagaaatc atgccaaatg aaacaatgca agaagatatc aacacgaact caggaaaagc
    14281 aatgccagaa tttcctggct atactgggga tgctaatcca gctgtacaag tagtgaaagt
    14341 caaagcagtt actcaccgaa aagacaatcc aatcatgcaa acaacgattg gtcccagtga
    14401 agaacacgta tctatggccg gtattcctac agaagcaagt attcttgcct tggtcgataa
    14461 agctattcca ggaaaagtag taaatgtcta caatcctcct gcaggtggtg gtaaattgat
    14521 gaccatcatg caaatccgta aagaaaacga agcggatgaa ggtattcagc gtcaagcagc
    14581 tattcttgct ttatcagcct tcaaagaatt gaagacagtc attttagtcg atgaagatgt
    14641 tgatattttc gacatgaacg atgtagtttg gactttcaac actcgtttcc aaggagataa
    14701 agatatcatc gtgatcccag gaatgaggaa ccatccattg gacccatcag aattaccaga
    14761 atataatcct ggctctattc gtttccgtgg gataagtgca aaaactatct ttgacggtac
    14821 agttcctttc gatatgaaag aacattttgt acgtgcaaaa ttccaagaag tagaagattg
    14881 ggaaaaatat ttgaaataat gaatgatctg caactgtttt gacatcataa aaaggagtgc
    14941 ggaaaatgga acaattggca caacggaaga aaaaaatcgt gattggtgta agtggcgctt
    15001 caggcaccat ttatgccatc aacctagtga agaaactaaa agaatatccg atgatagaaa
    15061 cacacggaat catcagtcca tgggcaaaac agaacttaaa gctggaatcc gacttatcac
    15121 taactgaatt cgaaagcctg tttgattacc attattcaaa caaagattta ggtgctgctg
    15181 tcgccagcgg atcatttttg accgatggaa tgatcatcgt accggcaagt atgaaaacag
    15241 tcgcaggtat ctctgttggt ttaggcgaca acctgattgc acgtgctgct gatgtaacaa
    15301 tgaaggagca acgaaagtta gtcattgttc ctcgtgaaac gccattaaat gccattcact
    15361 tggaaaactt gactaaatta gcacgactag gtgtgcaaat cataccacca attcctgctt
    15421 tttataatca gcctcaaacg atccaagatc ttgttaatca ccatacaatg aaacttttgg
    15481 atcaatttga aattgtaaat gagcattcaa aacgttggca gggtgattaa tatggaaaaa
    15541 gaaacgatag ttgaagtaac caaactaggt ttcacgatcc aagctgctgt taaagtaatc
    15601 ggtaaagact tattgatcac tttaacaggc ggagatacac cacatatcgg aacagtcact
    15661 acattttctc gtgatacaga gatgcaaact gtacgtttcc ctagccacga tggtcggttc
    15721 cataaagacg atgtcttagc cgaaaaaata gctgtgatca tcaaaactgc cgttccggga
    15781 agttgtacga tcacttccgg tatccatgtt gaccatatct ccaatgcaca aatcaacgcc
    15841 tcttttccga tgtcagaaga gttaggttac aagattttag attggttgga aaaaatagat
    15901 ttccatatca aagaaccagt ttattataaa gatggtgaaa aaccattatg aaagaagagg
    15961 ttgtgaaatc agcgttatga cctgagtatg agagaaaaaa atggaaaaat agcttctcat
    16021 attttgccgt ttttgagcta ataccgaggg acagctgatt gaacgccgtt tatcaagagg
    16081 ttgtgataaa gccgcttcgt ttcgagtagt aagaaaaatt ttgtaaacat gggtttccac
    16141 atttttgcat aaatttgact tactaccgag aaactggctt ttgaacaccg tttatcaaga
    16201 ggttgtgaca cgtcttttgt cacaacctct tttatttttg cttcactgta actgctacaa
    16261 tatcaccgat ttcttttccg atgtttttac gaatatcttt tcgtagtcca aggatgtagc
    16321 agactgaacc atctggattt ttcacgccca tattcacgat actgccttca tacggcacat
    16381 tgtcaaaagt tgcttggact ttaactcttc cagcaccgaa ttcttttctt atatcatacg
    16441 gaaaaataat atatgccccg cccttgccga cttcggaagc atagattttt gattcaaaag
    16501 aataaatttc cctcatttta tttctcctat tttttacatc tagtatagca tagcaaaaaa
    16561 aaagaaggaa ctgtgacaaa agtcttgtcg cagttccttt tttcgaataa acggtgttct
    16621 cctgccaact cctcggtcac aagtcacaat agtcaacaaa catgagaagc tgttttttga
    16681 aattgctcct tgtgactcag agttaaacgg caggctcaca acctcttcct taaacggtgt
    16741 tcaaaagctt gcatctacgg cactaagccc aatcagatga aaaatatgga gtgctatttt
    16801 tatctgattg ttcttactgc ttgatgcaga gcagcttttt cacaacctcc tacatggtgt
    16861 tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg ttgttggaat
    16921 tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt tggattttca
    16981 gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc catcgtactt
    17041 tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa ttctacccat
    17101 gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt atcagcaata
    17161 ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt cgtcactttt
    17221 tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc agttgctttt
    17281 ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc attccctaaa
    17341 gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat tttttctgtt
    17401 tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac gatcaaactt
    17461 agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca tgttttttta
    17521 gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc aataatcatg
    17581 cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag gcaataataa
    17641 agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct gacagtgtgg
    17701 cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat ttgcttggga
    17761 tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca atcgagcttg
    17821 cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta tttggcaatg
    17881 cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac atacggatcg
    17941 cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca aatggtgcag
    18001 ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg tatttatcga
    18061 aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc aacaacatca
    18121 agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt acgaccattt
    18181 ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc gtgaatgtag
    18241 atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga cgccatgaga
    18301 acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag cttccccagc
    18361 cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc gttgataaaa
    18421 tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc attaggaaga
    18481 atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag tcacgccaat
    18541 ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca agtccaataa
    18601 cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata gatagcatga
    18661 tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa cgaccgatta
    18721 gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa aagatcgtca
    18781 tggcagaaag atataaattt cttgaactga atttattgat cagccatgct gttattggga
    18841 tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca gacgctgaaa
    18901 tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga ttcaaaacgg
    18961 tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat ggttttccgt
    19021 aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt tttctgaaac
    19081 tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac ataagttaat
    19141 acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt tggggcttcc
    19201 cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt aagtgatatc
    19261 gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat tcgttcgatc
    19321 gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa agtagaactg
    19381 atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa ggcagaaatc
    19441 cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa agatatcgaa
    19501 ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga gaatgctaag
    19561 atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct tcctgtcgat
    19621 gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat ctcctaagta
    19681 ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg tttgaacata
    19741 agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc catcactgtt
    19801 cggatatttt tttatttttt tcggttactt tcttctaata catctgttag ccaatttgca
    19861 tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta attttcatgt
    19921 ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag tcgtttttct
    19981 tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt cacgtctttg
    20041 acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt ggtgggtgta
    20101 tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac tttcttttcc
    20161 aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc catttttgca
    20221 agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc atttttgaac
    20281 gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa caaaatatat
    20341 cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta aatccattct
    20401 atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta taaggagatg
    20461 cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact ttatctatac
    20521 gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg ttgattaccg
    20581 tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg atatcgttaa
    20641 attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag acgtggcatt
    20701 ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta aatgggttga
    20761 agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa tggaagcagc
    20821 tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta cgatcactta
    20881 tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat acgatggaat
    20941 gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag taaaataagg
    21001 ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa agaaacggaa
    21061 aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc tgattgaaca
    21121 ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa cgctagttag
    21181 aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca gccgtttgaa
    21241 caccgtttat caagaggttg tgaaat
//
LOCUS       DACCQI010000051        20817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCQI010000051 DACCQI010000000
VERSION     DACCQI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.052"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(39..1240)
                     /locus_tag="IVO76_001453"
                     /pseudo
     CDS             complement(39..1240)
                     /locus_tag="IVO76_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1357..1872
                     /locus_tag="IVO76_001454"
     CDS             1357..1872
                     /locus_tag="IVO76_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7429422.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2071..2517)
                     /locus_tag="IVO76_001455"
     CDS             complement(2071..2517)
                     /locus_tag="IVO76_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7429423.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IVO76_001456"
     CDS             complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IVO76_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7429424.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4396..5025)
                     /locus_tag="IVO76_001457"
     CDS             complement(4396..5025)
                     /locus_tag="IVO76_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7429425.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5009..5473)
                     /locus_tag="IVO76_001458"
     CDS             complement(5009..5473)
                     /locus_tag="IVO76_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7429426.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5960..7228)
                     /locus_tag="IVO76_001459"
     CDS             complement(5960..7228)
                     /locus_tag="IVO76_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7429427.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7459..8196)
                     /locus_tag="IVO76_001460"
     CDS             complement(7459..8196)
                     /locus_tag="IVO76_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7429428.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8263..8832)
                     /locus_tag="IVO76_001461"
     CDS             complement(8263..8832)
                     /locus_tag="IVO76_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7429429.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8960..9832
                     /locus_tag="IVO76_001462"
     CDS             8960..9832
                     /locus_tag="IVO76_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7429430.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10174..11046
                     /locus_tag="IVO76_001463"
     CDS             10174..11046
                     /locus_tag="IVO76_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7429431.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11043..13001
                     /locus_tag="IVO76_001464"
     CDS             11043..13001
                     /locus_tag="IVO76_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7429432.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13252..14103)
                     /locus_tag="IVO76_001465"
     CDS             complement(13252..14103)
                     /locus_tag="IVO76_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7429433.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14226..14693
                     /locus_tag="IVO76_001466"
     CDS             14226..14693
                     /locus_tag="IVO76_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7429434.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14956..15438)
                     /locus_tag="IVO76_001467"
     CDS             complement(14956..15438)
                     /locus_tag="IVO76_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7429435.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQEVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15538..16401)
                     /locus_tag="IVO76_001468"
     CDS             complement(15538..16401)
                     /locus_tag="IVO76_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7429436.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            16999..17847
                     /locus_tag="IVO76_001469"
     CDS             16999..17847
                     /locus_tag="IVO76_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429437.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18018..18359)
                     /locus_tag="IVO76_001470"
     CDS             complement(18018..18359)
                     /locus_tag="IVO76_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP7429438.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18445..19152
                     /locus_tag="IVO76_001471"
     CDS             18445..19152
                     /locus_tag="IVO76_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7429439.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19139..19462
                     /locus_tag="IVO76_001472"
     CDS             19139..19462
                     /locus_tag="IVO76_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7429440.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(19551..20588)
                     /locus_tag="IVO76_001473"
     CDS             complement(19551..20588)
                     /locus_tag="IVO76_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7429441.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
BASE COUNT         6201 a         4309 c         3695 g         6612 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt ttcaaaccga tccgactaga taagaaacct gtaaatagca
     1021 tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag
     1081 cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag
     1141 cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg
     1201 tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct ctattagcat
     1261 catacatgat ttattttaca tgtaaatatg cacatgtaaa acgtggaaac tttactgtga
     1321 tataataaag aaaataagac gagaggtagg atttttatga atactttagc ctatatatta
     1381 ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt
     1441 ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt
     1501 tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat
     1561 gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa
     1621 gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga
     1681 ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg
     1741 aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga
     1801 attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag
     1861 ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc
     1921 ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc
     1981 tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc
     2041 caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac
     2101 acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat
     2161 tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac
     2221 accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat
     2281 catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac
     2341 accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa
     2401 caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac
     2461 catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct
     2521 gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa
     2581 gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa
     2641 agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta
     2701 aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac
     2761 ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac
     2821 ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa
     2881 gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca
     2941 tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt
     3001 ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa
     3061 tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac
     3121 ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt
     3181 ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt
     3241 ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt
     3301 gtttagagga agacaaccga tccggtctga tttgttgctt tttcttttta ctggacactt
     3361 tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct
     3421 ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga
     3481 gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt
     3541 tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat
     3601 aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct
     3661 cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt
     3721 cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg
     3781 ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga
     3841 aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga
     3901 gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga
     3961 cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta
     4021 tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat
     4081 cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta
     4141 ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca cctattctac
     4201 tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg
     4261 aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc
     4321 cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata
     4381 gtaactgaaa accttttatt cgaacgtagt gatcttttct ggtgctaaac gaacagactc
     4441 gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt
     4501 atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc
     4561 atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt
     4621 gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa
     4681 ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt tatagatttc
     4741 ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc
     4801 attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc
     4861 agcgaccaca aagcgccatg gttgcatatt gacagaagaa ggtgcagtcg tcgcttcttt
     4921 gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga
     4981 tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt
     5041 tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt
     5101 gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca
     5161 tttcacaatg ctcgattttc ttctgggctt tttctgttag cgctacatac acttcccggt
     5221 tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac
     5281 gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat
     5341 tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt
     5401 caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct
     5461 cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa
     5521 taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc
     5581 aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa
     5641 agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg
     5701 cagtagaaaa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg
     5761 aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg
     5821 tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac
     5881 caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca
     5941 gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca
     6001 tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt
     6061 catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt
     6121 cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat
     6181 ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc
     6241 aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca
     6301 atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag
     6361 ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac
     6421 cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt
     6481 tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct
     6541 tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg
     6601 cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc
     6661 cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt
     6721 tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg
     6781 cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag
     6841 cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt
     6901 gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct
     6961 ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt
     7021 cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac
     7081 tctctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt
     7141 ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa
     7201 cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc
     7261 taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt
     7321 gtttcttttt gataaatttg ctttttctac ataaaaagac gtaaatgaag aatattcttc
     7381 tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta attctctttg
     7441 gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca tgttactgac
     7501 agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcggaggt
     7561 caatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt
     7621 ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg
     7681 ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata
     7741 aacggcggaa tcaggataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc
     7801 agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat
     7861 gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg
     7921 catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc
     7981 gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc
     8041 ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag
     8101 tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc
     8161 catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa
     8221 atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac
     8281 aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt
     8341 aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt
     8401 tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct
     8461 tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa
     8521 tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa
     8581 gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt
     8641 caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg
     8701 ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttcagctt ggatctgatg
     8761 gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat
     8821 gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg cttatcatgt
     8881 ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt
     8941 taaaaacagg agggaaaaaa tggatattcg tatactgacg tattttttag cagtagctag
     9001 agaaaaaaat atcagccgag cagcaaaatc tctgcatatc acgcaaccta ctctctcaaa
     9061 acaactgaaa gatttagaag aagaacttgg tgtgacactt ttcactcggg gctcccgaga
     9121 gatacagttg acggattctg gtcgatatct ttattcaaaa ggaaaagaaa tcttgtcgtt
     9181 ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc atctctggag aaatctacat
     9241 tggcggaggc gaaactaaag caatctcttt catttctgat agccttcatt cgttgatcaa
     9301 ggcgcatcct gatatccagt ttcatttata tagcgggaat gcagatgata tcatagacaa
     9361 actggatagc ggattacttg attttggact cgtaatcgaa ccgacaaaca aacaaaaata
     9421 ccagtcattt cgactgccag cccaagatcg ttggggactt ttgatacatc gaaatcatcc
     9481 tttagcaaag caaaccgtca tccatccgca ggaactgaaa actgttcctt tgatcatttc
     9541 ccggcaaacg tcagtggaca gtcaactagc tgaatggcta ggtttcagca tagatcagtt
     9601 atcgatcgtc ggaacatata acttattgta caatgcttct ttgatggtcg ataacgggac
     9661 ttttggtgca ttagctctag atgggatcat caatacttat gggacggaaa aaatattcat
     9721 cccactcgat ccgccattac aagcttcact gaatctcatt tggaaaaaag accagcctct
     9781 ttctcctgct gctcgtaaat ttttagatgt cctaacggag aaatacagtt aagtagagtt
     9841 tttttccttc gctttcagct ttttaacaac gaataagata agcaaaagat ccaacgatta
     9901 aataacataa gtttcttaat ttatttcttc cttttatttc tgataattcc agtaaattac
     9961 tataattata aaaaaagaat tgacaaaaca taactgtcta tataaaattg attacgttaa
    10021 caataattcg tttcatgtga ctagatcata ctaggcatga aaaatctctg aggaacatgg
    10081 tttccctagg aggtttttcg tgtctatttt tgtttggccg caatcaactg gtctagcaaa
    10141 cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa tcaaaaagat ctttaatcaa
    10201 aatgcagtat tggtagatga cggtggacag gaaaaagtag ctattggaaa agggattgga
    10261 tttgacagaa aaagaaatga tttgattttt gatcgtgatg tcgaacggat atttgtcatg
    10321 gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc agattgatga acgcttttta
    10381 tttgcagcag aaacgatcat tgatcatgcg gaaactgttt tgatggaaaa gttgaatgaa
    10441 catgtattga tcgctttgac cgaccacatt gctttttctg cggagaacat taataacggt
    10501 atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc tttacagtga tgaattcagt
    10561 attgctcaat gggcagtaga gtatctgaca aaagagcttg ggattccata tacttatgac
    10621 gaagcaggat atatcgcaat ccatattcac agcggacgaa gcggtcggaa caataaccat
    10681 cgcagtatcc gtgaagtgac gattgtatct gacatcatcc atttagtgga atcagaattg
    10741 gaaacagata tccattcaga accattttca ctgaattatt caagactggt gaatcattta
    10801 cgactgcttc tacaacgaac ccatgcgcaa caatatgctg ttttagacac ggaaatcgta
    10861 gaaatggtca aacgaaaata cccagaaagc tataagattt ctaaaaaaat ccgtgtgttg
    10921 ctgaccaaag aataccagct cgcgatcacg aaagaagaac tgggttactt ggccatccat
    10981 atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta acaatcatga ggaggaaaaa
    11041 aattgaaagc atatctacaa cgaatgggac gttcccttca acttccagtt gcggtcttac
    11101 ctgctgctgc tttactagta gggatcggaa actggtgggc atcttatagt aacgacattg
    11161 tcgctcattt tttacaagct ggcggtaacg cagttttagg tcaattacct ttgctgtttg
    11221 ccgttgggtt agcactaggg atgtcagtgg acaaatcagg tgctgctgcc ttggcaggtt
    11281 tggtcgcatt tgaagttcca gtaaatgttt taaaaactga ttcagtagct actttattga
    11341 atatcaaacc agaatcagtt gatccatctt ttgctcagat cagtaacgta tttattggta
    11401 ttattgccgg attgattgcg gcagctcttt ataatcgttt ccacgaagca aaattaccga
    11461 tggctttatc tttctttagc ggcaagcgtc tagtgccaat cttggctgcc tttgtcatgt
    11521 tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt ttactctgct ttagtttctt
    11581 ttgctaaatt cattttgagt ttaggctggg gtggtgcagg tctttatggc ttcttcaacc
    11641 gtttactgat ccctacagga ttgcatcaag caatgaactc cgtgttctgg tttgacgtag
    11701 caggaatcaa tgatattggt aaatttttgg cttctgaagg aacaaaaggc gtaacaggaa
    11761 tgtatcaagc tggatttttc ccaatcatga tgttcgggct tccagcagga gcatatgcta
    11821 tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc ttccttgatg ctagcagcag
    11881 cttttgcttc attctttact ggtgtgacag aaccacttga attttcattt atgttcgttg
    11941 cttggccatt atatgtcttg catgcaattt tcactgggat ttctttagca gtgagtgcat
    12001 tcttccattg gacagcagga tttgccttta gtgccggatt tgtcgattac ttcctatctt
    12061 tgaaaaatcc gattgcgaat cagccattga tgttgatcgt ccaaggatta gtaacagcag
    12121 tgatttacta ctttggcttt aactttgcta tcaaaaaatt ccatctgatg actcctggcc
    12181 gcgaggaagc agatctttca gatgacgata ctgcaacgac taattcagac aataaatatg
    12241 cggcacaagc aagcaaaatc tacgctgcat taggcggagc cgacaatgta acttcgatcg
    12301 ataactgtac tacacgcctt cgtttgcaag tcaaagatac aggcctgatc gatcaaaata
    12361 aaatcaaagc cactggcgta cctggtatga agatcatcga cggaaaaaat gcgcaagtca
    12421 tcgtaggtac agaagtacaa ttcgttgcag atgagatggc aaaactacat ggtggtgctg
    12481 cagcacgacc tgcacaaaca aacaccgtgg ttaaaacaga aacatttgcc ggtgaaacga
    12541 tcgaacaaga tatttacgct atcgcaaatg ggaaactgat tccaatcact gaagtttcag
    12601 atgatgtatt cgcggaaaaa atgatgggtg acggctatgc tgtattacca gaaaatggag
    12661 aaatcttctc tccaatcgct ggtacgatca caaacatttt cccaacgaaa catgctgtcg
    12721 gaatccaaac agatgctggt atcgaagtct tgcttcatat gggcatcaac actgttgact
    12781 taaaaggcga accattcact ttatatgtag aagaaggtca aaaagtcgca agaggtcaat
    12841 tgattgcttt agttgatctt gccgctattc aatctgcagg caaaaacacg gatatggtcg
    12901 ttgtattcac aaatggcgac aaagtccaaa gcttagaaat cgaacccgca cgtgatgtaa
    12961 aagcaaatga taaaatcggc tctgtttcca acaaagcctg agccatcgtc ttccccagac
    13021 ttttttatcg ataaacaaac aggagctgag acaaaaatct tgtctcagct cctttttccg
    13081 aataaaaacg gcgttctcct gccaactccg cggtaataag tccagctaaa cgaaacatat
    13141 gaagagctat tttttatttg actcttctta ttacttgatg cagaacagct ttttcacaaa
    13201 atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg
    13261 atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc
    13321 taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa
    13381 catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc
    13441 acttgcttca ataatttcta actctttacc tgtcgcacgc tctaatgcct tggctaattc
    13501 ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac tttctactag
    13561 tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt ttccatttcc
    13621 tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta attcattctc
    13681 tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc
    13741 agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt
    13801 ataagcgata taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc
    13861 ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc
    13921 tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag
    13981 tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt
    14041 ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg
    14101 catatttgcc actccctatt ctttttattt gttaagattt acagctgtac atttataaat
    14161 tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac
    14221 aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa
    14281 tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg
    14341 cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa
    14401 ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct
    14461 atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt
    14521 gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa
    14581 aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt
    14641 tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc
    14701 tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg
    14761 acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca
    14821 tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca
    14881 aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc
    14941 acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt
    15001 cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc
    15061 tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg
    15121 cgattctgct aaagcgctta caacaagata cgttcctttt gggaatggga tgattcgcgt
    15181 agtgtttgga acctcctgac tcgtttgaac accgacatag tacatcattt tcccgttata
    15241 gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt
    15301 tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc
    15361 cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc
    15421 gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa
    15481 attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta
    15541 tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca
    15601 ttttgtaaaa gagatctcgg gagcttcgac taacccataa tctgaagaag gaagccattc
    15661 tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa
    15721 aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt
    15781 tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc ggtctccatc
    15841 aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc
    15901 ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc
    15961 aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat
    16021 acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga
    16081 tttttgtatg ccgtatctac tgatttctga aggcagatat cctgaccaat cacgaaaagc
    16141 tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga
    16201 tccatctaga agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag
    16261 cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt
    16321 ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa
    16381 ttggttcaga tctttaagca tatttatctt cctccttgat tgccttttta tcatattatc
    16441 aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt
    16501 atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct
    16561 atctatgttt tacctttaga aaaataaatt ttggggaaga cagattctaa gtaaacaaag
    16621 cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa
    16681 ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc
    16741 tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata
    16801 aataaaaaac aaaaaaagtt ccatctttaa aacacgctat tcttttactt tttctcacat
    16861 tctctaagaa aagaaaagga gaatgaattt ttgacaattc gagcaaaagt gattatcaaa
    16921 tatgtaaaat cagctacttt tttcttaaac gtttctttat actaaatata aataagtgtt
    16981 tcatgaaagg agaactagat gaataattgg cttgagtatt ttcctgaaaa cgttttggaa
    17041 cgcggttaca gctatcatct gcacggtttt gtccgtcatt tgaactatac aagcaagtac
    17101 ttgtccgcaa cagtttctgg cacggaagat tataaggtag tgatcacttg ggatgaaaag
    17161 accaacatga cttgtgattg tctatatgcc atagaaggaa aaaaatgcaa acacatggct
    17221 gctgtcttat tcgcttacga agaacgtcca ataaaaaaat ccaactactc actttccgaa
    17281 ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac gggagctttt aacagagatt
    17341 cttatagaac atccgcactt tatcgaacgc ttcaaagtta agatgccatt tcatgcaata
    17401 aattattcag ataaattaac aactatcatc cataaatacg atcatattat caagaaaaac
    17461 aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa agttcatcca agaagcagtt
    17521 gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg aattgatcaa tgaagtgatt
    17581 gctactctcg aaacattcta ttgggagcct gaggatgaac gaacccttct tctgatagag
    17641 gactgttatt atttatggaa agaattatta gcagaagcac ctcatgcaga aaaaaggcaa
    17701 atgttctctt ggttcgtctg ccaagtcgat catactgacg cctcttattc aaaacgctac
    17761 tctataaaaa tcctgaaaga agactttcgg gaaaaagaat tttctaacca aaagaaaaaa
    17821 atagataaaa aactaaagaa acggtaaaga aagacgattt caatgaaaaa tagcaagtaa
    17881 acgataacaa ccatcctaaa tccacaagaa atacagcaga ccttgcttta aaaacacgaa
    17941 tcatgcaaag gatgaagtaa agaagactta acgcgtaccc aatattagtc atttgactat
    18001 taggtacgcg ttttgtttta taagtcatga caaatttcct tcgttgctgg atagattttc
    18061 cggcattgtt gatagatttt atgatacttt tctacatttt gtaagagagg ttccgctgca
    18121 tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg tgtcgaacca ccctaaccct
    18181 agtgcagcaa tcattgttgc gcctaatgca ggtccttcct ccatacttaa acaatagacc
    18241 gttgcatcga aaatatcagc ttggatttgt agccatgcaa gatttttcgc gcctcctccc
    18301 accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt tacgtttcaa tcccaccatt
    18361 gacaaatgct cattaaaaat ttagaatatt cacaagttta acgacaatta tgaaaattta
    18421 tgtcgtgcta aaggagtttt gttattggat gaaaagttag atataaaaac tgcgatcaaa
    18481 gacacgttac ctaccgtttt cggttatatc ggtattggac ttgcatttgg tatcgttggg
    18541 aaagctgccg gatttcatcc actagtcgtc acgttgatgt ccctgctggt ctatgctggt
    18601 tctgcccaat ttatcacagt cagcatgctt gctagtcaca gcccattgct ttccatcgtt
    18661 ttctcgacct ttctagtcaa ttcccgaatg attctgatga gtatgacgat tgctccttat
    18721 ttcaagaaaa atcggctgct tcaaaatctg ctgattggca cattgctgac agatgaaagt
    18781 ttcgcactgg gaatgaataa attgaactat actggacaaa agttaaactt tcgttggatg
    18841 aatacagcta attggatttc ttatttgacg tgggtgggtt cttctcttgt tggcgcactt
    18901 ttagggaatt ttattactga tccgaaaaag tttggtttgg atttcgccat cgtcgctatg
    18961 tttatcgggt tactttatct tcaagtcatt tctgatagaa acacctctaa acgattacag
    19021 ttgatcctga ttggtctcac tttgattctg gtatatgtcg gactgatttt tatcccaagc
    19081 aacttagtga ttgtagttgt aacactgatt gggtgcggat tgggggtgtg gatcaaacat
    19141 gccttctttt gagtttacat tgctgactat tttaggttgt actttagcaa cttggctttc
    19201 tagggtccta ccatttgtct tactgaaaaa attcgattta ccgcaaccgt tgcttgagta
    19261 tttaagtttt gtcccgatcg tcattatgtc tgctttatgg ttcagcagtc tatttaccca
    19321 aaatatcgga catctgccgc agatcaatct cgaaaatgct ttggcttcag ttcctacact
    19381 tttagctgct attctttcaa aaagcttatt ggtgatcgtc ttggcaggaa ttctttccct
    19441 ttcgttgatt cgcttattat aaataaaaaa acaagagctt atggcaaatt actgtcatgc
    19501 gctcttgttt ttatcaaagt caaataagaa aaaaatcggc gtattcttgc ttatccgttg
    19561 aacggaccac taacaacgac ttttcctaac atatgtcctg tttctacgtc tttggttgct
    19621 tgtttcaagt tcttcgcatt catcccatct gaatagacct tgctcaatgt actgcggata
    19681 atcccttcat ttgctaaagc agcaatgtgc gctaaagctt ctccttgcgt ttcgatttca
    19741 tagttatagt ctgttttagc gaacatatac tcccagtcga agctgataga ttggtttttc
    19801 caaacaccta tatctaacgg ctctttgatc cctacgatcg ttcccacatg tccaaatggt
    19861 cggacaaggt gcttgatttg atcaaagtag cgggtaatat cgaataatac agcgacatac
    19921 tctacttgat cgattcctaa ttcacgaaaa cttttactta agtcgtgatg ataatcgatt
    19981 ggatagtcta cacctgtttt tttcagccat tcaaaattct tcggacttgc agtcgcgtag
    20041 acttctaggc cagcccaatg cgctagttga ttcaaaatcg aaccgacgcc cccggatccg
    20101 ttgatcacta agattttttt cccgcgattg gcattttctt ctggagttag accaaatttt
    20161 tcaaataaca attcataagc ggtcaatgag gtcaacggca aggctgcagc ttcttcatct
    20221 gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa ctagttggta ttcttgattg
    20281 cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat cgccgacaga aaatttttgc
    20341 acttcgtcac ctacagcaac aactttcccg actccatcaa aacctaagat acgtaaagca
    20401 tttcttatcc cgttatcttg gcgaagtttc gtatcgacag gattaacaga tactgcgttg
    20461 acttttacca gaagatcccg tgaactgggt acaggtatct gttctttttc atcaataaag
    20521 ctttttggat catcgatcgg caacccttca taaaaaccga ctaccttcat taattccttt
    20581 tctgacatac gaattcctcc ttagcttcat caagttggag catccataca gtgttcattt
    20641 caatatatat agtatgctat ttgttccacc ttttttattc atcataatta ttctcttact
    20701 taaagtaaac tatttgcact tttttggtta aaaaagaggt tgcgacaaga cttttgtcac
    20761 aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc acaagtcaaa atattca
//
LOCUS       DACCQI010000052        20796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCQI010000052 DACCQI010000000
VERSION     DACCQI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.053"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..623)
                     /locus_tag="IVO76_001474"
     CDS             complement(<1..623)
                     /locus_tag="IVO76_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7429442.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFV"
     gene            complement(726..1166)
                     /locus_tag="IVO76_001475"
     CDS             complement(726..1166)
                     /locus_tag="IVO76_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7429443.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(1223..1633)
                     /locus_tag="IVO76_001476"
     CDS             complement(1223..1633)
                     /locus_tag="IVO76_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7429444.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(1813..2247)
                     /locus_tag="IVO76_001477"
     CDS             complement(1813..2247)
                     /locus_tag="IVO76_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7429445.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(2347..3195)
                     /locus_tag="IVO76_001478"
     CDS             complement(2347..3195)
                     /locus_tag="IVO76_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7429446.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            3584..5350
                     /locus_tag="IVO76_001479"
     CDS             3584..5350
                     /locus_tag="IVO76_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7429447.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            5452..6711
                     /locus_tag="IVO76_001480"
     CDS             5452..6711
                     /locus_tag="IVO76_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7429448.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            6858..8159
                     /locus_tag="IVO76_001481"
     CDS             6858..8159
                     /locus_tag="IVO76_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7429449.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            8162..9013
                     /locus_tag="IVO76_001482"
     CDS             8162..9013
                     /locus_tag="IVO76_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7429450.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            9026..9841
                     /locus_tag="IVO76_001483"
     CDS             9026..9841
                     /locus_tag="IVO76_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7429451.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            9992..11158
                     /locus_tag="IVO76_001484"
     CDS             9992..11158
                     /locus_tag="IVO76_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7429452.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            11388..12035
                     /locus_tag="IVO76_001485"
     CDS             11388..12035
                     /locus_tag="IVO76_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7429453.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            12119..13063
                     /locus_tag="IVO76_001486"
     CDS             12119..13063
                     /locus_tag="IVO76_001486"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7429454.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            13198..14649
                     /locus_tag="IVO76_001487"
     CDS             13198..14649
                     /locus_tag="IVO76_001487"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7429455.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            14670..15506
                     /locus_tag="IVO76_001488"
     CDS             14670..15506
                     /locus_tag="IVO76_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7429456.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            15506..16165
                     /locus_tag="IVO76_001489"
     CDS             15506..16165
                     /locus_tag="IVO76_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7429457.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            16291..16980
                     /locus_tag="IVO76_001490"
     CDS             16291..16980
                     /locus_tag="IVO76_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429458.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            16986..17942
                     /locus_tag="IVO76_001491"
     CDS             16986..17942
                     /locus_tag="IVO76_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7429459.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            17986..18498
                     /locus_tag="IVO76_001492"
     CDS             17986..18498
                     /locus_tag="IVO76_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7429460.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(18754..19302)
                     /locus_tag="IVO76_001493"
     CDS             complement(18754..19302)
                     /locus_tag="IVO76_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7429461.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(19391..20572)
                     /locus_tag="IVO76_001494"
     CDS             complement(19391..20572)
                     /locus_tag="IVO76_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7429462.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         6907 a         3728 c         4016 g         6145 t
ORIGIN      
        1 acaaacttat cggcaaaata gtaaatatct tgaaagaacc aagaaaattc actttttaaa
       61 aacaatcttg agaatacaat tgtttttttc ttttcaatca taaaaaagaa acttaagatg
      121 aatgacatag caaacgccac tgccattttt cctatatcct gtaaatagcc aagcgcaatc
      181 gaagcgccat tttgaagctg ttcaaaaaaa tcgtttcgct caatataagt atggatatat
      241 tgtaaaacag gatctgcatc ttttggctga tgctcgtaaa aattaataac ggactggatc
      301 atgtcaaatg tttgatgaat aagcatcggg atatactttg tgaatgccag atagataagg
      361 taaacgatca gtgtataaat catgatagct aaaacacctg tagggattcg gacaaatcgc
      421 tgaatgattt gaacaagatg aatcatcaaa taagtaaaga taaatgtgag caaaatggtt
      481 gtaatcatac ttctaactaa gaaaagaaca aaaacgacaa ataataaaac agtgagacgg
      541 cgtatttttt cattcgccaa aaaacgctga taaacggtca aatcgacaac ctccaaataa
      601 caaaaaataa acacgttatt cattatatga tggaatatga aaataagaaa gagcaaaaag
      661 aaaaaccgct aaatgacgtt atcttatgag ttggttttcc tcataaaatc gttcatctag
      721 cagtttcagc cctttttctg gtcttgatga aaattatcta gcatttttcg ggtcagatgt
      781 tgcagtgtat ccaattcttc ctgttcaagt ggaagataag acttgatctt ttgtggaata
      841 ccttctgctt gtttttgcaa cgcagcacct tgagcagtca gcgtaacatt tacgattctt
      901 tcatcacccg tttcacgctg acgattaata taattttttt ctgctaactt ttttagtaag
      961 ggagtcagcg taccagaatc cagacacaaa tcatttccta gctctttcac tgaagtcttt
     1021 ttcttttccc ataaaactaa catcacaaga tactgggtat aggtaagatt cagcggcttg
     1081 aggaggggag tgtagtgtct aatgatttct ttagaggcgg catataaagg aaaacatact
     1141 tgattaacta aatatagatc ttccattttt ccacctccgc tgttttacta tagtgtatca
     1201 tagcggattt acacaaaaaa gtctattcac taactgcttt cagagtaact tctatatttc
     1261 cagctgtagc ttttgaatac ggacacactt tatgcgctgc ctctaataat tcttgcgttt
     1321 cttccagaga aagaccttcg atagatcctt caatctctac acctaagaca aagctcgttt
     1381 cactttctga ataaagggaa acttgtgcac tgacagtaga agccccagag atattttttt
     1441 gctgtttgat caattctaat gcactgttaa aacatgcact gaaaccagct gcaaacaatt
     1501 gttcagggtt ggttgcattt tcgacgcgtt taccaggagc tgcaatattc aattcgaatg
     1561 tacgatcggg attatgcact tctccagagc gtccgcctgt gttgatcatt ttcgtagaat
     1621 acattttttt cataaaatta ccacctttaa ttatatttta tacaattgaa ttttacacaa
     1681 tataattgtg gaaagcaaag aaatggattg gcataagata aataagagaa agcacatgaa
     1741 acagacgcgc tttctcgaat agcaaacata tacaaaaaat atcagagaca aaaaataatc
     1801 tcatcataaa cattacatat atttctcaaa aagttgtttt ctttcttcaa aaattacttc
     1861 acgatggacc tgatgaagca tccataaata accaaaagga tctctgaaca ttgagttagt
     1921 cacaccaaag tccatcattt ctgtcatttc ttgaatctct acacagccat ttcgtaatgc
     1981 tttattatgt gtgtcctcga tatcagagac gatgatattg aaccagatcg tatttggatg
     2041 ttcagcagta ggagcagtca aaccaagaga cgtgttttca tccagcatat ggaatctcgt
     2101 accatacaag gtaaaaatga cttcattatt gcctctaggg taatctgttg cctcaatcag
     2161 ttgtatttca tcaaagattg tttgatacag ttctagtgct ttcaaactat catttaatac
     2221 aaaatcgatt tccgttcctt tatgcataaa gatctcctct ccttttcagt cgaacagtta
     2281 ctagattctt ttattatacc agctaaagaa aacgcattca aaaaaatagc ggatttactt
     2341 accaactcat tcgattggat gataagtaaa tccgcgtccc attacttctg ctacgctgct
     2401 gacaatgaca aaggcacttg gatcgatttc ttctatcaat ggtttcattc gggaaaattc
     2461 atgatcagtc acaacgatca ataaaacctc ttttgacttt cgattatacc cgccttttgc
     2521 ctctagcagg gtaaggccac gtccaatctc agcacttaag cgaaggcgga tgtcttccaa
     2581 ataatgttca ctcatgacca taacagattt ctttcgattg atacctgttt cgatataagt
     2641 catgacaatt gaagtgattg caatagataa aatagcataa agaaactctt ctattccaaa
     2701 aataaacaga ttaagagaaa caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt
     2761 caagtggaaa tattttttaa aaatgagggg agggatcgtc gtaccgccgc tagaagagtt
     2821 gtttttatat aaaatcgcaa ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa
     2881 caaccgatct gaagtaatca ttgtttctgg aactattgca atgattaaag gaaaaaccaa
     2941 gcttccgaaa agtactttaa aaaagggttt tttacctaaa aatagaagag ctagtaccaa
     3001 cataacgata ttcaacacta aaataactat agctcggtca aagcctaaag cagcttccag
     3061 taatattcct agaccgctga cacctccagc agcaatatga tgaggcccta aaaacatatt
     3121 gatagcgact cctaatatga agagggcaaa aataatctta atgtatgtcc atataaattg
     3181 tctcgtttct ttcatttatt cacttcctta attcattaat aatactctta tcttattata
     3241 acttactttt caccttaagt taactttaat taataaaaaa attaggctag tctaaattat
     3301 tatgtttcat tcttctagaa tatacttcta cgccaaaaac tgttaaaaaa cttttacttt
     3361 agaaaattgg agaaattgat cgcttaagat aaattcagga cactaaatag tacaggaagt
     3421 agactaaaac ttttaaaagt tttaagtcta tctatttggc aagtttaaaa aataaaaatt
     3481 cggtatcaaa aaactgttac cgataacatt tttgaaagcg ttgtcttttg cttctctatc
     3541 acttatagtg atgacgtcat attaaaacac aaggaggaaa tcaatggata cagctgcaat
     3601 ttatcatcga ccggaaagtg aatatgcatt tctatatacg aatacaagat ttcgtattcg
     3661 gcttcgtact aggaaaaatg atatcaaaaa agtatatgtg ctatgtggag atccatatac
     3721 cataacaact gaaaaatggt atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa
     3781 tgttcatgat tattgggaaa ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt
     3841 ccatgttgtt ggggaagata acacggactg tttttacggt gatcaaggaa tttttcctta
     3901 tcgagaaaat gtgattactg aaccaaattt ttattttcga ataccttatt ttcatcaaat
     3961 cgatcgattc actattccag aatgggtaaa agaaacagta tggtatcaaa tttttcccga
     4021 aagatttgca aacggggaca aagcaaatga ccccgaacac actttacctt ggggaagtaa
     4081 agatccagac cgagaagact ttttcggggg agatttgcaa ggtgtgatgg atcatttgga
     4141 ttacttgacg gaccttggca tcaatggcat ctatttttgt cccatattta aagccacatc
     4201 caatcataaa tacgatacga ttgattatta tgaggtggat cccgcttttg gcgataaaaa
     4261 acttttaaaa aacttgattg atgaagcaca taaaagaggg atacgtatca tgttcgatgc
     4321 tgtattcaat catatgggtg tccattcacc acagtggcaa gacgttctca aaaacggaga
     4381 aaaatcgatc tataaagact ggttccacat ccgttttttc ccagtagatt cttatcagat
     4441 gactgactta ccagaaactg ctgaaaatat cccatatgat acttttgctt ttactccatt
     4501 catgccgaaa ctaaatacag caaatccaga agtgcaaaaa tatttgctgg atatcgcaac
     4561 ttattggata aaagaattcg atattgatgg atggcgtctt gatgttgcta atgaagtcga
     4621 tcatcatttt tggaaaaaat tcagagaagc agttacagaa atcaaaccag acatctatat
     4681 ccttggagag atttggcatt cctctcaagc ttggctgcaa ggggatgaat tccatgcagt
     4741 gatgaattat gcatttacag attcgattaa agattatttt gcaaaaaaga aaatcacagc
     4801 ttcacaaatg gtcagtggaa tgaatcatca gcaaatgctt tacagagatc aggtgaacga
     4861 aggaacattc aacttattag attctcatga tactgccagg atcttgaccc tttgccaagg
     4921 taacaaagaa ttgatgaagt ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg
     4981 tatctattac gggacagaaa tagggatgac tggtgaagat gacccggatt gccgcaaatg
     5041 tatgatatgg gagaaggagg aacaagatct agagctattc ggttttataa aagaacttgt
     5101 ttctctaagg aaacagttgt ctaagattat ttctgaaggt tctactcagt ggctaatagt
     5161 aaatgatcgt gaagataagc tctatttcac acggaaacta gagggacaga tcatctatgt
     5221 ttactttaat cagtcaaaag aaccttgggt agtagaacag gaaaatgagg tgattcttag
     5281 tcaaaattgt cagttgttag aagacgggaa agcagagata cgacaatacg gatttttgat
     5341 catgtgttga aaactgttac cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc
     5401 ccattataat aagggtgtaa cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa
     5461 agaagtgtga aacagttggg tttagggtta atctctttag gaatcttagg cggattagcg
     5521 gcttgtggca atggtaactc ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca
     5581 gtttccgttg attctggata taaagattat ataaacgaaa taaaaaacac tttcgaaaaa
     5641 gaaaacgatg tgaaaatcaa gctagtagaa aaggacatgt tcgatcagtt ggaatcatta
     5701 gcgttagatg ggcctgctgg aaagggacct gacgttatga tggcagcata tgaccggatc
     5761 ggtgcgttag gacagcaagg acatctggcg gaagtaaaac tgggaaatga agcagcttac
     5821 gatgaaacag acaaagccca agtgacttat gatggaaaaa tctacggaga acctgcagta
     5881 atcgagaccc ttgttttgta ctataacaaa gatttagtcg atacagcacc atcaacgttt
     5941 aaagatttgg agaatctatc caaagacagt cgttttgctt ttgagtcaga agcaggaaaa
     6001 aatacaggat ttttagctaa atggactgac ttttattatt cctatggttt aattgcaggt
     6061 tacggtggct atgtatttgg tgatgatggc acaaatcctt cagatatcgg tttgaataat
     6121 gcaggtgctg tagaaggaat ctcttatgca acagattggt tcaaaaatgt ttggccaaag
     6181 ggaatgcaag acataaaaag tgcaggagac ttcgccagcc agcaattcat gtccaataaa
     6241 actgcagcaa tcattgacgg tccatggcaa gcgcaaacat acaaagaaaa caatatcaat
     6301 tatggtgtag ccaaaattcc tactctaaat aatggacagc cttatcaacc gtttggcgga
     6361 ggaaaaggat gggtagtcag caattatgct aaaaacaaag acctctctca aaaatggctt
     6421 gattatgtaa cgaatcagga aaatcaagaa aaattctttg aaatggtaaa tgaaatccct
     6481 gcaaaccaac aagctaggga aacagcaaag gctaaaaacg atgaattaac aacagctgtg
     6541 attgaacaat atgaaacagc tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg
     6601 acaggtgcag aaaacttgat gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc
     6661 gcagatgaag cagtaaaaac aatcagtgaa gccatagaac aaaaatactg attaaaatga
     6721 taaccgtcca tttttataaa aaatggaaca ggtgcttcag ataaaagtgg caagagcgag
     6781 atggaaaatt atcttgctct tgttgccatt atcttaaaag cagtttgcgg tttagcaagt
     6841 ttagaggggg cattcagatg cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca
     6901 cgctttgttc acttattcca gggatggggc agtttgtaaa tggccaagct tttaaaggta
     6961 tcgttttttt cgccatattc gtcctattca ttggtcagat gcttctgggc ggatttcatg
     7021 cattggtagg attggttact ttgggaagta taccaatgga agatcattca ttatttattt
     7081 tgattgaagg cactttgcaa ttcatcatta caatcatttt cttgattttt tacggtttga
     7141 atcttttaga cgcttatcga gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga
     7201 cgatgaagga agtcatacat aatataggtg atgaaggttt tccttatcta ttgacacttc
     7261 ccgcttatct tctcatgatt tttacgatca ttttcccagt cctagttaca ttgttcactg
     7321 catttactaa ttatgacttc aatcatattc caccagccag tttaattgat tgggtagggg
     7381 ctaaaaactt ttttagtatc ttctttttaa gctcatatcg taatactttt tttgcagtct
     7441 tttcgtggac cgtgatttgg actttatgtg caacaacttt gcaaatcacc cttggtattc
     7501 tcacagcggt tgttgctcac cagaaattta ttaaattcaa acgtattttc ggtatcattt
     7561 tcttattacc atgggcggtc ccagctttta ttactatcat gagtttttcg aatatattca
     7621 acgatagcgt aggtgccatc aatactcaag tgattccttt cataaaccag cttccttttg
     7681 tagatatcag tgcaatctct tggaaaacgg acccgaactg gacgaaagta gcaattatct
     7741 tagtgcaggg ctggttagga tttccctata tctatgtgat ggttacagga atcttacaat
     7801 cgatttcaga agacttatat gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct
     7861 tttcaaacat cacgcttcct gctgtcttcc tagtggcagc gccaacattt attacacagt
     7921 atacggggaa tttcaataat ttctccatga tctatctgtt taacaatggc ggtcccggaa
     7981 gcgtaggcgg aggagctggt tctactgata ttcttatttc ctggatctat aaacttacga
     8041 ctggaacttc tccgcagtat tctatggctt cagcaatcac gttgattatc tccgtcatcg
     8101 ttatctgtgt gtcattgctg gtatttaaaa agacaaatgc gttcaatatg ggggattaac
     8161 aatgaaaaaa tttcattcga gtgctcgatt ccaaaaacga ctttctcact tttttactta
     8221 cttttttctt ttaatactgt cagtcattat catctatccg cttttgatca cagcaagtac
     8281 tgccttcaag tcaggaaata ttgcagcttt tagtttagat ctttcaagtg actggacttt
     8341 aggaaacttt actcggctat tcagtgaaac actttacggg acttggtata aaaatacatt
     8401 gatcatttct gtcctaacaa tgatcgtaca agtgactgta gtaactttgg cgggctatac
     8461 ttatagtcgc tatcgtttta tcggccgcaa atcaagtttg atgttctttt tagtgattca
     8521 gatggtaccg actatggcag cattgactgc cttttatgtc atggctttct tattagaagc
     8581 gctcgataag tactggttct tgacaatgat ctatataggc ggtggaattc caatgaatac
     8641 ttggctgatg aaaggatact ttgacactgt accaatagat ttagatgaat ctgctaaact
     8701 tgatggggca gggcatttca ggattttttt ccagattgta ttaccgcttg tcaaaccgat
     8761 gctcgccgtt caagcattat gggcttttat gggacctttt ggggatttca tgttagctaa
     8821 gttcctgctt cgaacacctg aaaatcagac tgttgctgtc ggcttacaaa catttatttc
     8881 tgatgtgaca aaccaaagag tctctctttt tgctgctggg gcaattttga ttgcactacc
     8941 gatttgtcta ttattcttct ttttacaaaa gaactttgtt accggcctga catctggcgg
     9001 aacaaaagga taggagcaga gtatcatgca aacatttccg ttaaactatt tttccgcatt
     9061 acgtacgcca actcaagtgt ttgcgggacg caaactatta tcatggccta aatttttctt
     9121 gatatttgta tttttagttt ccttgatggt catgcctgtg actctttttt atgccaatca
     9181 gatccaagcg attccattag aacaatttct atccgttcat tcgctgatcg atgaacaagg
     9241 aacccaaaaa ttttctgaac ttgaattatc tgaaactggg ttacaagcat cgcaacaaac
     9301 gatagctgtg acccctgaaa ttttggtagg tgtgtcttta tctgagaaac aacaatccga
     9361 tcatggaact tttattgatt ttgagaagga acaatgggtg atccaacaaa aggataaatc
     9421 tggtatacgc cgctatacga tgaattatag cccatctttc caaccagatt ctgtacgtac
     9481 gcccgaagat ttccaacgat ttttagaaag agaattttat gccagtaatc gtccaacaat
     9541 cattttatct tatagtttaa gtcttggtct tgtcctattt gtcatgacga gtctcatttt
     9601 attcggggct tcctttttct tgtggatgac aagaaaaagc caactttcat ccattcatac
     9661 attcaaagag agtgcaaatt tgatgctgaa tgttatggga ataggttcca tggcagcagc
     9721 agttgtaggg tttatccact ttgactttat cttgatgcta agtgtccaaa caacgataac
     9781 tgtcttgctg cttttatggg tttttgtcaa aacaagattc aaagataagc gtgtcgaatg
     9841 acaagtttaa gcgatttatg cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt
     9901 tgaaaaattt ctgaaaaaat taaacttctt agttgattct tttctttttt agtattaacg
     9961 taatagggta gtttttttaa ggaggaaaat catgttagag aaaactttat acaatcaatt
    10021 attttcacgt tctttctcgc tccctgtcga agtaaattat tgggacggta caacgaaaca
    10081 atacggagat acggacactc ctccccaaat caaaatcaat atccatgaag aaatcccaat
    10141 gaaagagatt acaagtaacg catctttggc actaggagaa gcctatatgg atcatcgaat
    10201 cgagatcgag ggaagtatcc aagcattgat caacgatgct tatcagcaag caaacagctt
    10261 tttacgagga aaagattatc taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa
    10321 aaacaaagaa gatatccatg cgcattatga cttaggtaac gacttttata aactttggct
    10381 agatccaact ttgacttatt cttgtgctta tttcaataca cctgaagata cgttggaaca
    10441 agcacaaatc aataaagtcc atcatatctt agacaaactc tttatcaaag aaggcgaaac
    10501 gttgcttgat atcggttgtg gctggggaac attgatgttc actgctacca aagagtataa
    10561 tgtcaaggca actgggatca cactaagtga agaacaatac gatttgatta ccaaaagaat
    10621 aaaagaagaa catttagaag ataaatgccg tgtattatta atggattacc gcgaactaaa
    10681 aggcgaaacg tttgatcata tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa
    10741 tcttgaaggt tatttcaaag tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat
    10801 ccatggaatc agccgacaac aaggcggtgc gacaaatgct tggatcaata aatatatttt
    10861 ccctggcggt tatgtccctg gtgttactga attagttgat aatattacaa aaaacgattt
    10921 gcaactgatt gatttggaaa gcttacgtcg tgactaccaa ttgacattag aacattggac
    10981 aaagaatttt catcaagtcc aagatcaagt cacgaaagaa atgggtgatt ccttctatcg
    11041 aatgtgggat ctatatttac aagcttgtgc agcttcattt gaatcaagta atatcgatgt
    11101 gattcaatat ttgcttgttc aacctggaaa caatgacatc ccaatgcatc gtgcataatt
    11161 gaggcaaaag tactgtttaa agactattta aaaagaagct aagttttatg atgctccaga
    11221 agtgaacttt tttgtctaac ttctaggatt gtctctaaag cttagtttct ttttctttat
    11281 attttcataa taaaagtttg gttctttaat ttttaaacat gacagaaaat tttttcttcg
    11341 ttatacttgt aatagaatac tattttataa caaaggggat cgaatcaatg aaaaaaactg
    11401 ttatttatac tagtttattt gcagctttgt ttttagctgc ttgtcaatca aataccaatc
    11461 aaggcacatc tgggaatacg tcttccagta gcgaacagcg ctcttcacag gtatcggact
    11521 cctcaacaaa agcaagcaca tcttcgtcta gccagacaaa aagttctagt gaagcaagta
    11581 ctcagtcaga tatcaaagtg tctgcagctc gtgcgatcga attgttccaa gataaatacc
    11641 cagatactgc aattactagt ttggaattag atagtgattg gggatcttac ttctacaaga
    11701 tagaaggtgt cgatgatcaa aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa
    11761 tggagacaga gaatccagaa actctagatc gagatgaaca aaatggtcaa aagaaaaaag
    11821 aagatggtct ggatgttgat aaattgatca cgattgagga agcagcaaaa atcgccgtcg
    11881 acaaagtagg aagcggtacg gcaacagatt gggatttgga taaagattta ggtactactt
    11941 attgggaagt caaagtaaaa gacggcaatc aaagtacatc agtcaagatc aatagccaaa
    12001 caggcgaagt actgtcaact gaacaagatg attaagaatt tcttcggaaa acaagagttg
    12061 atttttcttt tagtcatgat acactagaca aaataacttg tttgaaggag cgatttactt
    12121 gagtttagaa acgacctttg ccaatcatat ctttgccaat cctttgatga atgcatctgg
    12181 tgtccactgc atgactaccc aggaattaga tgaactggct cattcagaag caggagcatt
    12241 tatcactaag agctgtacga tcaatgaacg aaaaggaaat cccgaaccac gctattttga
    12301 tgttccttta gggagtatca attctatggg acttccaaac ttaggttttt cttattatct
    12361 agagtatgct cttgcgtatg aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc
    12421 cattgccggg atgagtgtcc aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg
    12481 gtttaatggg atcactgaat taaatttatc ttgtcccaat gtgcctggaa aaccacaact
    12541 agcttacgat tttgaggcca cttatgaaac actgaaagaa gtattcagca tcttttctaa
    12601 gccgctagga atcaaattgc ctccttattt tgattttgct cattttgatc agatggctga
    12661 tatcttgaat cagtttccat tgacctatgt caatgctatc aacagtgtgg gcaatggttt
    12721 gtatatcgat acagaacaag aagcagtagt catcaaacca aaagaagggt tcggcggcat
    12781 tggcggagag tatatcaagc cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt
    12841 gaaaccagaa atccaaatca ttggtacagg tgggattcgg acaggacaag atgcatttga
    12901 acatctgcta tgtggcgcaa gcatgttaca gattggtaca gagcttcata aagaagggcc
    12961 ggaaattttt tctagaatca taaaagaatt aacgcagatt atgtcagaaa aaggatatac
    13021 gtcaattgac gaatttaaag gcaaattacg caccatttca tagcaaagag acgaaggtca
    13081 tgattgatct tcgtcttttt ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta
    13141 ctcattgtat gttagacgaa cttgtgggaa aataacagag gcaaaaagga ggataatatg
    13201 aatgatgcaa cagaattagc aaaagagctt aaagaaaaag aatttagttt tcaagaacta
    13261 gttacggata tacagaaaaa aataaagaag caaaatcatg aattgaatgc gtttgtcgcg
    13321 cttgaaccat tcgagagtaa ttcccacgag ctattgattc cccagtcaga aaaagggcca
    13381 ttggctggta tcccatttcc tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct
    13441 acttcaggat ctcgtttatt tgaaacacac cgagcttctc atacatctaa ttatgtgcgg
    13501 caagcagaag cgataggtct agttcctttt ggacaaacca atgcgccaga gtttggtttc
    13561 aaaaatatta cagacccagt catctatggc ccagcaagaa acccttggaa ccttgaatgt
    13621 acaccgggcg gatcaagtgg aggagctgct gctgcagttt catcaggaat cgttccttta
    13681 gctggtgcta gtgatggcgg tggttctatt agaatacccg cttcgttttg cggattgatt
    13741 gggctgaaac cttcgcgagg gacgatgcca gttggtccat atgcttggag aggatggcaa
    13801 ggagctgcta ttgatttcgg cttgacagtt tctatgagag acacggaggc tttattttat
    13861 gggatgagaa gtatccattc cggtgcgcca tatcaagtat cgccagttga gtggcaaaca
    13921 catccaagaa aaaaacggtt gaagatagct ttttgtgttg attcaccgat ccactcacag
    13981 attagtgaag aggcagtact tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg
    14041 catgatttga cagagattcc ttacccacta gatggtcgta aactgatcac gtcttattat
    14101 cagatgaatg gcgcagaaac agcagcaatg atccgctcga ttgaaacagg catccagcga
    14161 gctgtcagaa aagaagaact tgaacccatt tcttggactt tgttccaata tggtcagaag
    14221 ctctctgccg ctacttacat cgattctctg catgtatggg atgaagcagc aattgtcatg
    14281 gaaaacctac atcaagaata cgatctattc ctttcaccaa cgactgcatt taccgcccct
    14341 aaaatcagcg aagatttgca aagtgatgcg ataagagcac agatggaaca catcagtgat
    14401 tgttctgaag tagaaggttt agacgttgtt tcttccatgt ttgaggagag cttgaagatc
    14461 acaccttata cacagttggc taatctgact ggacaaccag caatcagctt gcctactcat
    14521 ttgacaaaag aaaacatgcc tctaggaatc caatttatgg ctgccagagg aagagaagat
    14581 ttgctatttc aaatcggcta tctatttgaa gcaaatcatc agttccattt accaaatcca
    14641 tggaaatagc agaaacaagg agaagaagaa tgaagaaaaa aagattgttt gctctaataa
    14701 ctagtgcctt ttttatttta agcgcttgcg gtacagctgc cgctaatcag gaagataaca
    14761 gctggagtaa tgtagaaaaa aagaaagaat tagtagtagc gacttctggc acactgtttc
    14821 ccgcatctta ttataatgat caaaatgaat tagttggtta tgatgtagat ttggcaaaag
    14881 aggtggctaa acggttaaat gtcaaaatct cctttaaaga atacaacgtg gatggacaaa
    14941 tcacttctgt ttcaaaaaga gaatcagatt ttgctgcgaa cgattttggt atcacaaaag
    15001 aaagagaaaa aaaattctcc ctttcagaga ctttcaaata ttcatttgac agtatgatcg
    15061 tacgaaaaaa agatgattca ggaatccact cacttgaaga tcttaaaggg aaaaaagcag
    15121 ctggcgaacc caatactagc tatatgcgtt tagcggaaaa acacggagct aaattagtca
    15181 catatgacaa tgccacgaat gatcagtatt taagcgatgt agcaaatggt cgaacagatg
    15241 ttattctaaa tgattattat ctgcagaaaa tggccgtatc tgctttacca gatatccctg
    15301 taaaaatact ggagaacgtt tatttcaatg aaaacgaaag tggtttttta ttgaaagaag
    15361 gaaatgatgc tctgaaaaag aaaatcgatg gcgtattaaa agagatgaaa gaagatggga
    15421 cgacaaagaa aatatctgaa aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa
    15481 cgatggctga gtcggtgagt gagtgatgta ctttcctcta attgattttt ccctcatgtg
    15541 gacttcactt cctttcgtac tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc
    15601 gtttctttta ggaaatttgg tagggttgct tttgacagtt ttaggtcttt tggattggct
    15661 gcctctgaat gtatttattc gtttttattt atcgttcttt agaggcattc ccgcactagt
    15721 cctgctgttt ctgttatatt tcgggttacc ttatcaatta agtgccttaa cggcttcagt
    15781 catctgcttt acgattacca gcagtgcatt tattggtgaa atctatcgag gatcactagc
    15841 aggcgtttct tcaggtcagt gggacgctgg tttagcttct ggtttttctt tctttggggt
    15901 attgaaatat atcgttttgc cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt
    15961 agctatggat ctagtgaaag gtacttcgtt agcagcaaca atcacagtgc cagaaatatt
    16021 tcaaaaagca aagatcatag gcggtcggac attcgattat ttttctctct atattttagt
    16081 tgcactgatt tattgggtca tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa
    16141 acgttctatc catcagtcaa tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt
    16201 tctatttttt gtttcttgta tattctgcct agtattttaa aaccatttca tgtaaaatgt
    16261 aaatcgaaaa tatgttcgga ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa
    16321 tcctcgttat accaatattg catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg
    16381 cctgatgacc aaggaattgt agatacgctt agctgcttga caaaatttga tggaagccga
    16441 gaatggcgat gctatgagct gaaagtgaca aaagcagatt ttcgctctac agccaaactg
    16501 tcatttgttg gacattataa ttattttgtt ttaccaagta aactatatga agaagtaaaa
    16561 actgaaatcc ctacaggaat aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag
    16621 atgcctgcct ctggtacatt tctgattgcg aaaaaagcag caaagaaaga gcttgctgta
    16681 ccggaagaag ctttgacaaa ccgcttcatg gcttctttat tcagggaagt aagaaaagca
    16741 aagcaaatgg agcgaggcgt aaaatatttt ccaaaagatc aattgtacaa agaattgaaa
    16801 agacgttcag aaaatcaaga tccattttct aacgaacgat attatgaacg gtttttagag
    16861 gaaatagcta gtcaggcaat caatgagttg caagaagaag ttatcgcgct tcagcatgat
    16921 tatgaatatt tgaggcaaca acagcagatc cgtcggttgc ctacagagcc attggagtga
    16981 aaaatatgta ctatccttat ttaagaggaa aacaatttga cctattagca ttaaaagaag
    17041 cactgtcgcg aggattgctc tccaataaaa tccaaccagt gatcgaacca gtacgtgatt
    17101 ccgctacgct taaaaatgtg atcgaattat ttcaaaaaaa gcaccatcca atcgctgtga
    17161 tccaaaatcc ccaagtagga caattcaaat tattcgatca gcatgtgcac agttggacag
    17221 tcaaagaaaa tagctctgtc gtccctgcac agatcttaac gccagaaaat ctcagtgaag
    17281 tactggattc acctccagcg tttcttgttt tcgatggaca gcactaccca aaagatacag
    17341 aggtgtggaa acagttggca ggagttgatt cgaaattttt gataccagat actagtcgct
    17401 ttcgcatctg gctgcccgaa aacaaaattg tgatccgtga ctcttttcaa acgagaaagc
    17461 atgttgaaag ttatgcagat aaaacagatg acttttttag tgatgactat ctttttttcc
    17521 acgcagacgg atatattgga ttttctgatt tcacgatcga aggtagccgt tatttcgata
    17581 aagggttccc tagccgagcg cttgctatcc atctaacgta tattgatgct tacggcaata
    17641 tacgcgtgaa acattttgtc tctgacagta acgactctgc taaagaccaa gcattgaagt
    17701 ttcttgaagc aggagataaa atgaaaaaat ggatcatgcg tcaccatcat caactcttga
    17761 ttacttcagg aatgattgaa ttattggctc tttaccagca acaaaaattc cctggactgg
    17821 gagtgttgaa aaagtggtca cttatgcatc atttggaact tattagtcac ctgctagatc
    17881 atccaaaaga ttggcttaaa tattattcaa aagttcagga actatatgaa gtgatccaat
    17941 gattagataa tgtaaatgaa aaaggaatag aaaagaggaa actggatgta tattcggcaa
    18001 acaaggaaag aagaagtaga agaagtggta gaaatcatta atgccgcagc ggatttcatt
    18061 gcttcgcaag gctctcctca atggcaaaac gggcagaaac caacaaaaga aacgattttg
    18121 aacgatattg aaagaaaaga aagctatgta ttaatagtgg aagatcaaat tgctggaaca
    18181 gctgcattag tctcaagcgt ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa
    18241 tcaactggtc cttatttatc gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa
    18301 aaactagcaa aaccgttctt agaacatttg ttgacgattg cagccagcaa aaatatccaa
    18361 gatatccgta tcgatactta tccagctaat gaaccgatgg aaaagaccat ctattctgct
    18421 ggattcactt atcaagggat gattgaattt ccttttgaac acggcgaaag aaaagcatat
    18481 caaataatca tcaattgact ataaaatcat ctgagcaaag ataaagacat taacactact
    18541 gaaaaaaaca atcatatgat agccaagagg ttgtaacaga agtggccagc ttcaaaaaac
    18601 aagaaagaat ttccgaaaat gcttctcaaa tttttgtgga attttagcgt attttacgaa
    18661 atagctgctt tctgaaactc ccttcatcga gaggtcgtga catagctttt gtcacgacct
    18721 ctcggcttat cctacgagta ttattcgata catttatatc aataatcctt tttttatttt
    18781 atctgcagct tcttggaacg ctccatcgtt tgacacgact aaaagtgagg ggatagcttt
    18841 caaacgtttc aactgtgcta aatcttctac ataaaccgcc agtctggctg aaatctgttc
    18901 agatgaatct ccacgttttt tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa
    18961 gataggacgg actaccgtcc cgaaatactc atataaatgc caaaaaccat ttgctgtcaa
    19021 aacggcgtaa caatttccta ctgtcagttt ctttttaatt tctgctttac tggaaccata
    19081 tttatgttga gcatattggt cccactctgc aaactcattt cccttgatca tctgattgaa
    19141 tgtattgtca gaaacaaaat aataatcctc ttgatctttt tccccaggtc ttggtgatcg
    19201 attcgtgtaa gtaatgatct tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt
    19261 tccagaacca gaaggaccga taaatacata gcaataattc attgtcattt ctcctttgat
    19321 gattgaacaa gtgaaaaagc tttggaaaat atttccaaag cttttaaaag taactttgct
    19381 tttttgtata ttataccagc aagccagtca caatagcact taaaaagctg actaaagtcg
    19441 ctgttagcaa aattttcaat ccgtgttttg cgacaagatt tcctttttct tcatttaatc
    19501 ccttcatcgc accagaaatg atcccgatag aagagaagtt ggcaaatgaa acgaggaaga
    19561 cagatataat tgctgttgta cgctcagtaa aatggaaatt accatttgct aattctgtca
    19621 tagcaacaaa ttcatttgta accagtttag tcgccatgat actaccggca tccactgctt
    19681 ctttccatgg gattccgctg atgaaggcaa gaggggcaaa gatgtaacct aggatctctt
    19741 ggaaagtgat gccgaatatc ccgtggaaga tagcattgat catggcaatc aaagcaacaa
    19801 aaccgataag catagcagca accgtgatcg ccacatggaa accatcaaga atgtattctc
    19861 ccaacatttg gaaaaatgtc tgttctttgt tttcttcaat gatcagctca tcttcttttt
    19921 cttctaatac atagggattt acaattgaag ccagaatata accgccaaat aagttcaaaa
    19981 caagggcagt gatcacatat ttagaatcga tcagtgccat ataagatccg acgatcgaca
    20041 tagaaactgt agacatagcc gatacactca tggtgaacaa acgtttctca gataggaagg
    20101 gtaattcctt ttttatggaa ataaaaactt ccgattgtcc taaaatagca gaagctactc
    20161 cattataaga ttccaatttg cccatcccgt tgattttact taatcctaga ccaacagctt
    20221 tcataaataa tggaagaatc ttgatatagc gtagaatacc gataatagca gaaataaata
    20281 caattggcat caatacggaa agaaagaaag gtgcacttcc tttatttgct acaccggcaa
    20341 aaacaaagtc aacaccttgc ccagcaaaag caagaagatg atcaaacatt tttgccaata
    20401 cagaaacaac cgctgtacca aaagttgtac gtaataatac gaaaccaaga acaagctgta
    20461 aaacgaacat cacaattagt ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa
    20521 aagcaagccc taatgtgaca aataaaccaa taatacctaa taaataactc aaataatcac
    20581 tcctattcca aattccgttt tattttacct gttttgcaaa taaaatacta atctttagtg
    20641 aaattttcat tactttttta gagtgaaacg cattcatttt ccattagaaa ataaagcgaa
    20701 caaaataaaa ggtttaaaaa gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat
    20761 cgattaagct aatagtttcg ttttcaacat ttataa
//
LOCUS       DACCQI010000053        20754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.054, whole genome shotgun sequence.
ACCESSION   DACCQI010000053 DACCQI010000000
VERSION     DACCQI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.054"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..315
                     /gene="sgrA"
                     /locus_tag="IVO76_001495"
     CDS             <1..315
                     /gene="sgrA"
                     /locus_tag="IVO76_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7429463.1"
                     /translation="SSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQ
                     PDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHR
                     VK"
     gene            613..969
                     /locus_tag="IVO76_001496"
     CDS             613..969
                     /locus_tag="IVO76_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429464.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            972..2129
                     /locus_tag="IVO76_001497"
     CDS             972..2129
                     /locus_tag="IVO76_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429465.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2155..2595)
                     /locus_tag="IVO76_001498"
     CDS             complement(2155..2595)
                     /locus_tag="IVO76_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7429466.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2755..3261
                     /locus_tag="IVO76_001499"
     CDS             2755..3261
                     /locus_tag="IVO76_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7429467.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3429..5342
                     /locus_tag="IVO76_001500"
     CDS             3429..5342
                     /locus_tag="IVO76_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7429468.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5347..6348
                     /locus_tag="IVO76_001501"
     CDS             5347..6348
                     /locus_tag="IVO76_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7429469.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6550..6756)
                     /locus_tag="IVO76_001502"
     CDS             complement(6550..6756)
                     /locus_tag="IVO76_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7429470.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(7402..8250)
                     /locus_tag="IVO76_001503"
     CDS             complement(7402..8250)
                     /locus_tag="IVO76_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7429471.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            8414..9316
                     /gene="gnd"
                     /locus_tag="IVO76_001504"
     CDS             8414..9316
                     /gene="gnd"
                     /locus_tag="IVO76_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7429472.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            9318..10319
                     /locus_tag="IVO76_001505"
     CDS             9318..10319
                     /locus_tag="IVO76_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7429473.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            10316..10600
                     /locus_tag="IVO76_001506"
     CDS             10316..10600
                     /locus_tag="IVO76_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7429474.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            10633..11430
                     /locus_tag="IVO76_001507"
     CDS             10633..11430
                     /locus_tag="IVO76_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7429475.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            11444..12277
                     /locus_tag="IVO76_001508"
     CDS             11444..12277
                     /locus_tag="IVO76_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7429476.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            12295..13152
                     /locus_tag="IVO76_001509"
     CDS             12295..13152
                     /locus_tag="IVO76_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7429477.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            13174..13683
                     /locus_tag="IVO76_001510"
     CDS             13174..13683
                     /locus_tag="IVO76_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7429478.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            13720..14121
                     /locus_tag="IVO76_001511"
     CDS             13720..14121
                     /locus_tag="IVO76_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429479.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            14233..14586
                     /locus_tag="IVO76_001512"
     CDS             14233..14586
                     /locus_tag="IVO76_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429480.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            14620..15063
                     /locus_tag="IVO76_001513"
     CDS             14620..15063
                     /locus_tag="IVO76_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7429481.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            15080..16165
                     /gene="uxuA"
                     /locus_tag="IVO76_001514"
     CDS             15080..16165
                     /gene="uxuA"
                     /locus_tag="IVO76_001514"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7429482.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            16158..16871
                     /locus_tag="IVO76_001515"
     CDS             16158..16871
                     /locus_tag="IVO76_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7429483.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            17623..17823
                     /locus_tag="IVO76_001516"
     CDS             17623..17823
                     /locus_tag="IVO76_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7429484.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            18519..18953
                     /locus_tag="IVO76_001517"
     CDS             18519..18953
                     /locus_tag="IVO76_001517"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429485.1"
                     /translation="MIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGND
                     HSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAV
                     LLNHVRKCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(19014..19379)
                     /locus_tag="IVO76_001518"
     CDS             complement(19014..19379)
                     /locus_tag="IVO76_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429486.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(19455..19700)
                     /locus_tag="IVO76_001519"
     CDS             complement(19455..19700)
                     /locus_tag="IVO76_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7429487.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(19770..20024)
                     /locus_tag="IVO76_001520"
     CDS             complement(19770..20024)
                     /locus_tag="IVO76_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429488.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(20188..20511)
                     /locus_tag="IVO76_001521"
     CDS             complement(20188..20511)
                     /locus_tag="IVO76_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429489.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT         7315 a         3218 c         4235 g         5986 t
ORIGIN      
        1 agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa tgaacttgat
       61 ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa cctaaaaagt
      121 gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa agcgaaagaa
      181 acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa caagctaatt
      241 acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg ttctctcttc
      301 catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac gatagatttt
      361 tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc tttgccagat
      421 ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca aatggaaaat
      481 aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa aaatcagtaa
      541 cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa tagataaaaa
      601 ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt atatcaattt
      661 ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat cgctttagat
      721 ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc gttttgcgaa
      781 aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa agtcgagttt
      841 tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac gattggttat
      901 attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct tagtcaaaaa
      961 attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact attttgaaaa
     1021 actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa gaataccgta
     1081 tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat tctgatatat
     1141 tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat gcgccatata
     1201 cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg caacgaatga
     1261 aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg catccattga
     1321 taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca aacttctggt
     1381 cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt ggtgtaatga
     1441 acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa agttggggct
     1501 ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt tttacaactt
     1561 cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca agggtttttg
     1621 ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat tttcagcata
     1681 tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct cgcagaattc
     1741 cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac tgtatgttag
     1801 tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa tcaaagtatg
     1861 gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa cgcccaataa
     1921 aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat cctatcgaca
     1981 aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa cctacaaaaa
     2041 atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa aatagatcca
     2101 tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc aaaattaaga
     2161 cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca agagttcgtt
     2221 gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt cgctaaatcc
     2281 agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag aaggggatgt
     2341 acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa caagattttt
     2401 ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca attttagtaa
     2461 gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga taaacggtat
     2521 atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag aacctgtata
     2581 caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat aacatgaaga
     2641 aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac gtataatgga
     2701 actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac aagtatgtca
     2761 aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt tgattcttcg
     2821 ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa aacatacgaa
     2881 acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt gccacaaaca
     2941 aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt tgtgagaaaa
     3001 acccatgcga attcatttta taagactaat ttgagtgttt tattaactga attggactgt
     3061 cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac tgtgaagttt
     3121 gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac ttatgataat
     3181 ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga taaacgattt
     3241 ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat ggatactggt
     3301 ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt tttatgggat
     3361 aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc ctagaaacat
     3421 aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg gtcatcttat
     3481 ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt tcttccgaat
     3541 cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa acgaaatcga
     3601 gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt actagcacag
     3661 atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca gctacaaaaa
     3721 tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg acagaaaatg
     3781 atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac ttcggtaaac
     3841 agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta atagagttat
     3901 tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc aaacatctga
     3961 ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg atgtcgatgc
     4021 ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat ccaaatacaa
     4081 aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat gtatatgcat
     4141 attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt cgagggaatt
     4201 ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg tataatttat
     4261 tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc tatggacaat
     4321 atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg ccatctcaaa
     4381 agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta ttagcgaaag
     4441 atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca attgatgaac
     4501 aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc ataaaaaatc
     4561 agttagacat cattggggat caagagcatg aagatttatt tatgggcctt ttgaaacaat
     4621 actattttga tcaggagatg catttggata caaaagctgc tctacctacg attggaatgt
     4681 cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta cctgtgtcta
     4741 acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta gtagaaggag
     4801 cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat gtaacggact
     4861 ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa aacaataaaa
     4921 gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa aattatatga
     4981 cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg actgaaggga
     5041 tttttaatga gacgatacca actattattt tctattacaa aaaaatccca gtagctccac
     5101 caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa cctcgagagg
     5161 atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa aaaaagacag
     5221 atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt ctcatttatt
     5281 caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga aagagagggt
     5341 aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat cgtagttatt
     5401 ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt gcttgttaga
     5461 aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga tttccagcaa
     5521 aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta caaaaataat
     5581 aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct tttctggatg
     5641 catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt ggaatatcta
     5701 tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc ggaagcaaaa
     5761 catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa agagataaaa
     5821 gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc acaaattagt
     5881 ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca tcttgatccg
     5941 actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt ggatccttct
     6001 gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg tggatgggct
     6061 tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc cttggtcaga
     6121 catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa tacgtttacg
     6181 aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt cacaaaagta
     6241 gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt cagcaataaa
     6301 caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag tcttatgatg
     6361 caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt caataaacaa
     6421 gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat ggcaaggggc
     6481 accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct tttacgtaaa
     6541 ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc cgaagcctgc
     6601 ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt ccactaatat
     6661 tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa tcaatgcact
     6721 agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct tttttataat
     6781 tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat gtgaataaaa
     6841 tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat gattcagcaa
     6901 tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa tatgggaata
     6961 taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga tgtatcctaa
     7021 acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa atgataacct
     7081 aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa atttttttta
     7141 attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat tttttactac
     7201 tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa tacaaccttc
     7261 tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg taccaccctt
     7321 gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt ttatggattt
     7381 ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc gaactttagc
     7441 gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata gagagtccaa
     7501 aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg tttcttcaga
     7561 aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt ggctagtaat
     7621 aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg tttcttttga
     7681 ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag aagcttccat
     7741 caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca aaaatttatg
     7801 ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga cacgtttaga
     7861 ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact tttttgtgtc
     7921 atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct cagtatctgt
     7981 tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga tcaacatggc
     8041 cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa aaaaagtaga
     8101 gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta ctttgcttgg
     8161 attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt tgtacattgt
     8221 tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata ataagattat
     8281 cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa atcaaacaaa
     8341 aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg gtataagaaa
     8401 tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat tgaatttagc
     8461 tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa gtacagctgt
     8521 tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat ttctagctac
     8581 ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa ccgaaaacat
     8641 gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag gcggaaactc
     8701 ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa tcggttactt
     8761 ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct taatgattgg
     8821 aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag caattgaaag
     8881 aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga tccataatgg
     8941 aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac aaaaaagtga
     9001 gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg tgattcgcgg
     9061 ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa gtaattatcg
     9121 gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc tagaaatggc
     9181 tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac aggaagaaga
     9241 tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac atgaaatagt
     9301 tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc agcagtttcg
     9361 gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt catttcttta
     9421 ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga ttcaatatca
     9481 ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa gatgaactgc
     9541 tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca gcaaaagaac
     9601 accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta gcagaaaata
     9661 atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca agagtcaagg
     9721 acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac aaaacagttg
     9781 gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt aacggttttc
     9841 gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa agctttggtg
     9901 caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt ttatgtgcga
     9961 atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag atgaaagaaa
    10021 atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg atgattaatg
    10081 gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa gaaccgggac
    10141 gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat atatcggcct
    10201 acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc gaacagatca
    10261 gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata aaaggatgat
    10321 gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt tatatgaaga
    10381 aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa gagtactggt
    10441 cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt tgaatgacga
    10501 aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg aagccagacg
    10561 aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg cggaggaaaa
    10621 ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg agtcatccct
    10681 actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa ggagaatgga
    10741 caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat aacagatccc
    10801 caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga tacactagca
    10861 aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga atcatttttg
    10921 aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca ttctgctaaa
    10981 gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct ttcagagatt
    11041 atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag aaatgcggca
    11101 gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg ttttctacat
    11161 ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg ctgggaaaaa
    11221 atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct agaacaaatg
    11281 ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat tgattcgaaa
    11341 gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca gtcaatgatg
    11401 gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa cattaagtgt
    11461 agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct ggttaggtgg
    11521 aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg tcgggttgat
    11581 catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga tttatatggg
    11641 ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag ctatcgcagt
    11701 accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg cggtgccagt
    11761 cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt ttttaggtaa
    11821 atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat caattattct
    11881 ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg ctttatattt
    11941 aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg ttatccacat
    12001 tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg tatacgttat
    12061 cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg ttgttttgaa
    12121 atctttagat attacgatgg ttacttatgc catttttggg attattatcg ctttgatctt
    12181 tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca attcagcatc
    12241 ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg ggaaatggct
    12301 gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga tgtaacaaaa
    12361 gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg ttacttggcg
    12421 ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga tgaagactct
    12481 ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag ctggggtggc
    12541 ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga agagtatgaa
    12601 gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg attgatgggg
    12661 gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat ttttatcgca
    12721 atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc ttttatctgc
    12781 tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt cactatgggc
    12841 cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga aatgctatca
    12901 gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt agaatcttca
    12961 ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga tcaaatcgtg
    13021 ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac gaaaaaagga
    13081 ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct agcaggaatc
    13141 ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt agcgcgtgtg
    13201 gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag tggagtgaat
    13261 tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga tctttacaaa
    13321 aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa agctaaattc
    13381 tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa aacagtccaa
    13441 acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat tggagggatt
    13501 gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt aaataaatca
    13561 gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat ttattttcaa
    13621 gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt tgatttgtca
    13681 taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa cgagctgttt
    13741 cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa aaagagtaaa
    13801 cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga tgtgacagtc
    13861 attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga tatcgaaaaa
    13921 gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg aagttatttc
    13981 atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt gtccttcatt
    14041 ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc ccatcacgta
    14101 ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag ctactttttc
    14161 ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg aaatacttca
    14221 tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt agcgtatgcg
    14281 tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc gggtatgaaa
    14341 aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct acagttttcg
    14401 ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt gaaagaaaaa
    14461 aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta tttgaccaaa
    14521 acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat tgctcttttt
    14581 cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat catgatcgtt
    14641 acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt cgggacatcg
    14701 agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt cggagagaag
    14761 gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt tgttgatcta
    14821 attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac tgaagaaaag
    14881 aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga agcaatcaat
    14941 catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa acaaggaaaa
    15001 gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga ggacgatttt
    15061 tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa aaggggattc
    15121 gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg gaactttgct
    15181 gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga aagcatctgt
    15241 tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg atgcaatcaa
    15301 ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa tcaaaaactt
    15361 gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg gatgggcgaa
    15421 aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg atcagaaaat
    15481 cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt caaagggatt
    15541 caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg aaaaactgta
    15601 tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag aaaaagtgat
    15661 tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc cgccatggga
    15721 actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa ttctaaatgc
    15781 agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg caaatccgga
    15841 aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg tccacttcag
    15901 aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct ctacagaagg
    15961 ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg acggtcctat
    16021 acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat atggcctgta
    16081 tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa gtaaggaaaa
    16141 accactggga gagcattatg aataaaatca agctcttcat ctttgatatg gatggccttt
    16201 tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa aagtatgatt
    16261 atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg gcgataagag
    16321 aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga gaagcaatca
    16381 atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa aaaggagcag
    16441 aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct tcgtctaata
    16501 cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg tttgatcaaa
    16561 tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt ttgaaagctt
    16621 gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg attgcaggga
    16681 tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat ttgatagggt
    16741 tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat gaatccaata
    16801 aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa agtaaagggt
    16861 tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc tgcacgactg
    16921 agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta tttgtagtga
    16981 acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg cgtttaaaga
    17041 gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt tttaattctt
    17101 gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct ttgtcaaaaa
    17161 cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct ttgttataga
    17221 atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac gcgttgataa
    17281 cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa gaaattttaa
    17341 agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat caatcgcggt
    17401 tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaattttat atttcattgc
    17461 attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta ttttgaaacg
    17521 taatttgagc gatatatcta catagataaa actccttttg cctatcaaat atttattgca
    17581 acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa cggtacagta
    17641 aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg aaatgatgta
    17701 tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga aggccaagca
    17761 gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat tgttaaagct
    17821 taataaaaat tgacttttaa ccgtcttttc gaaagaaaag acggtttttt tgtacgaaaa
    17881 aattcattgg tcgttgaatg ggctgaatag tagtgtataa aacaattttg tggagcgtga
    17941 agcttttatg gtagaggaaa atacgaataa agtgatgttg ataaaaagat tcctatcttg
    18001 aatatgaatc gagatgtctt gaaaatgaat gagtttgacc aatcattatc ctatcgaaat
    18061 ttacagtttc gttcgattgg tctgtcaatt aatgaaaaag taaaaaagaa tatgttctag
    18121 gaagagggcg gcacatctaa atttttaagt aacggacagt tcaaatatca agcattgaaa
    18181 gaggctatcc tactgtttga aggaatacga atatataaaa acgacaaaga ggaaaaagta
    18241 gttcattaaa tcccttcaag tgatgaaaga atgagatcta ataaaacctg atctttcatc
    18301 tgttttttta ataacgtaat aattttttta ttttttgtgc tttcgccgtt attgtagagt
    18361 gatttagcat tcttattttt aaaaataact gtgttatata ttaaaaaagg cataatagtt
    18421 ataagctttt ttaaagaata tttagaaatt attaattttc gtctgaaaac aaaaaaagaa
    18481 agcgttatta tttagtctat tttcaggggg ggaaccttat gataaaaaaa ctatttttac
    18541 tatcaatatt aacgctagca atggcaggtt gttctgactc tggcttagca actgaactga
    18601 tcacaaaatc aactgaagcc acgacagagg agttaaaatg tggaaacgat cacagtgact
    18661 acagaggctt aaatcgacga tgcatgggaa tattaggcag aagcaattgt aagcatcgat
    18721 gtagtaatcg aagaaggtgt agaagaagca acagaaaaat caaaagaaag atcaaaagaa
    18781 gcaatctgtg ctgtgcatcg tatacagacg tcgaagatgc ggtactttta aaccacgtca
    18841 ggaaatgttc atttatgtct agggaagttg gcgatgaacc aaaactagag tacgctttta
    18901 gttttgcacg tgaggatgaa gataacccta aaacttacga cattttagct tgatctaaga
    18961 tggttaaagc tttttaatga agtaagagga gatagaaggc ttttctagtt atattaggta
    19021 agataaattc ctttttgctc caaatttgct aaacattctg ctagataaac ttcatcgtgt
    19081 tccggataaa tcggcacgtt gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat
    19141 ttgccacgat acataggatt tttccactct ggtgctgggc tgtaaccacg tttttccatt
    19201 tcatccatga caagctgatg atattggaat aatctataag gagaatagtg aaaaacataa
    19261 tccacagtag catgtttctt tccccagcca tttcctctta atgcacagca ttcccgatgt
    19321 tgtcctaaaa gctgttgacg aggaagcttt gaaatcaatt tttcatgcca taatcgcata
    19381 gaaatgtctc ctttatatat agattgttaa ccttagacgt taaatgaaaa aaccagcacc
    19441 aaggctggaa aaaatcactt cagtttgctt gctgtttctg agcgaccaat taggtaatca
    19501 acggaaatat cgtaaaagtc agcgatatag attaattcaa tgatgcttgg aatttcttct
    19561 ccttcttcgt acttacgata tttgaataaa ggaatactta acgcgctaga aacagctact
    19621 tgagataaag atcggccagt tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa
    19681 atagcttctt cgatactcat ttaatcacct ttcattatag tagaatagtt ataatgacta
    19741 tagaaagcta cacatgcaat caatagtact caatgttttt tatttaaacg tatcaaataa
    19801 agaatgacga tcaataaaga caaaaccagt cctgataatt cttctataaa aacactatcg
    19861 ggttcaaagt agttgtaatt aaaaactttc cacataaagt agatagccgg aagagtcaaa
    19921 atagctaaat aaaaccgttc tttgttccaa ttcataagat aaacaatact tgcgaccagt
    19981 gcgaccgcag gcacccaaaa caaaactctc accaacgtaa tcatgtaacg gactcctcaa
    20041 cgattcatat aataaaagat aacatgttat tgcatacatc acaaatgaaa tgctaggaaa
    20101 ataaaagaag aatagatcaa tgaaaaatga aaaagcagaa cagttttctg ctgataagca
    20161 ggaactgttc cgctgaatgt gtcttatcta tcgagaaaaa atcaatggat tgatatctaa
    20221 gtaattcaat acatataaaa agaggacgat cgcattcact aaaaaggaaa gaatgatgat
    20281 cccatagcga atggaaacat atttaacaaa tacttttgtt tgaatacggg catatctttt
    20341 tcctttatag ctgagcatca tgacaagaag caaaagggta atcacacctt gaaaaatcat
    20401 tccaataaac agaattcctg aggtttgata atttaattgg agaaaataag ctgctgccgc
    20461 aactaaatcg aataaaatcg caataaatgg caaaataatc actcctaaca ttgagaataa
    20521 ataaaagaat aacatttttt ctaaaataag caaataaaaa ttaaagtaac aaaatagtta
    20581 tgacacaagt aaaagaaata agaataacaa tattatttta cttatatttt gaaacttgtg
    20641 agagtttgtc attcgtgcta tagttgaaaa gtaacaaaaa taaattttat ttatgatgta
    20701 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttc
//
LOCUS       DACCQI010000054        20500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCQI010000054 DACCQI010000000
VERSION     DACCQI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.055"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(105..629)
                     /locus_tag="IVO76_001522"
     CDS             complement(105..629)
                     /locus_tag="IVO76_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7429490.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IVO76_001523"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IVO76_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7429491.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IVO76_001524"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IVO76_001524"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7429492.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IVO76_001525"
     CDS             complement(2351..3031)
                     /locus_tag="IVO76_001525"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7429493.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IVO76_001526"
     CDS             3157..4026
                     /locus_tag="IVO76_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7429494.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IVO76_001527"
     CDS             4052..4567
                     /locus_tag="IVO76_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429495.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IVO76_001528"
     CDS             complement(4614..5177)
                     /locus_tag="IVO76_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7429496.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IVO76_001529"
     CDS             complement(5232..5411)
                     /locus_tag="IVO76_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429497.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IVO76_001530"
     CDS             complement(5488..5997)
                     /locus_tag="IVO76_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429498.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IVO76_001531"
     CDS             complement(6093..6473)
                     /locus_tag="IVO76_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7429499.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IVO76_001532"
     CDS             6803..7552
                     /locus_tag="IVO76_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7429500.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IVO76_001533"
     CDS             complement(7736..9013)
                     /locus_tag="IVO76_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429501.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IVO76_001534"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IVO76_001534"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7429502.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IVO76_001535"
     CDS             complement(9935..10879)
                     /locus_tag="IVO76_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7429503.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IVO76_001536"
     CDS             complement(10881..11567)
                     /locus_tag="IVO76_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7429504.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IVO76_001537"
     CDS             12041..12361
                     /locus_tag="IVO76_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7429505.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IVO76_001538"
     CDS             12515..13420
                     /locus_tag="IVO76_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7429506.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IVO76_001539"
     CDS             13417..14190
                     /locus_tag="IVO76_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7429507.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IVO76_001540"
     CDS             complement(14371..15795)
                     /locus_tag="IVO76_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7429508.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IVO76_001541"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IVO76_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7429509.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IVO76_001542"
     CDS             complement(16517..18499)
                     /locus_tag="IVO76_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7429510.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IVO76_001543"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IVO76_001543"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7429511.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IVO76_001544"
     CDS             complement(19412..20473)
                     /locus_tag="IVO76_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7429512.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
BASE COUNT         5920 a         4346 c         3633 g         6601 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct
//
LOCUS       DACCQI010000055        20200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCQI010000055 DACCQI010000000
VERSION     DACCQI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.056"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            86..1126
                     /locus_tag="IVO76_001545"
     CDS             86..1126
                     /locus_tag="IVO76_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7429513.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1224..1412
                     /locus_tag="IVO76_001546"
     CDS             1224..1412
                     /locus_tag="IVO76_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7429514.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1470..1823)
                     /locus_tag="IVO76_001547"
     CDS             complement(1470..1823)
                     /locus_tag="IVO76_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7429515.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2354..2776
                     /locus_tag="IVO76_001548"
     CDS             2354..2776
                     /locus_tag="IVO76_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7429516.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2773..3003
                     /locus_tag="IVO76_001549"
     CDS             2773..3003
                     /locus_tag="IVO76_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7429517.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3501..3701
                     /locus_tag="IVO76_001550"
     CDS             3501..3701
                     /locus_tag="IVO76_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7429518.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3728..4921
                     /locus_tag="IVO76_001551"
     CDS             3728..4921
                     /locus_tag="IVO76_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7429519.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4989..6554)
                     /gene="guaA"
                     /locus_tag="IVO76_001552"
     CDS             complement(4989..6554)
                     /gene="guaA"
                     /locus_tag="IVO76_001552"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7429520.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(6613..7536)
                     /locus_tag="IVO76_001553"
     CDS             complement(6613..7536)
                     /locus_tag="IVO76_001553"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7429521.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7732..8337
                     /locus_tag="IVO76_001554"
     CDS             7732..8337
                     /locus_tag="IVO76_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7429522.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8645..9661
                     /locus_tag="IVO76_001555"
     CDS             8645..9661
                     /locus_tag="IVO76_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7429523.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9802..11103
                     /locus_tag="IVO76_001556"
     CDS             9802..11103
                     /locus_tag="IVO76_001556"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7429524.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            11149..11811
                     /gene="deoC"
                     /locus_tag="IVO76_001557"
     CDS             11149..11811
                     /gene="deoC"
                     /locus_tag="IVO76_001557"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7429525.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11832..12230
                     /locus_tag="IVO76_001558"
     CDS             11832..12230
                     /locus_tag="IVO76_001558"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7429526.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            12296..13378
                     /locus_tag="IVO76_001559"
     CDS             12296..13378
                     /locus_tag="IVO76_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7429527.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            13590..15152
                     /locus_tag="IVO76_001560"
     CDS             13590..15152
                     /locus_tag="IVO76_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429528.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            15145..16278
                     /locus_tag="IVO76_001561"
     CDS             15145..16278
                     /locus_tag="IVO76_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429529.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            16278..17234
                     /locus_tag="IVO76_001562"
     CDS             16278..17234
                     /locus_tag="IVO76_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429530.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            17333..18499
                     /gene="deoB"
                     /locus_tag="IVO76_001563"
     CDS             17333..18499
                     /gene="deoB"
                     /locus_tag="IVO76_001563"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7429531.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            18523..19341
                     /locus_tag="IVO76_001564"
     CDS             18523..19341
                     /locus_tag="IVO76_001564"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7429532.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            19358..20065
                     /gene="deoD"
                     /locus_tag="IVO76_001565"
     CDS             19358..20065
                     /gene="deoD"
                     /locus_tag="IVO76_001565"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7429533.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6470 a         3622 c         4342 g         5766 t
ORIGIN      
        1 tgcatatata cgcctttata gtggagtact acatttacga gattcggtta gagtatcaga
       61 aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat tatgtaagat
      121 tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga agttaaatag
      181 tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc cgcaccctct
      241 actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct
      301 tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta cgacacatga
      361 aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac tgttgcaaga
      421 aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg agagaccgtt
      481 aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct gggcttccat
      541 tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga gctcggtttc
      601 tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac gctatggttg
      661 cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta agtacggttt
      721 atactatagc cctgttagta ctccagcaga ttttcggatg cttactccta ttgtactgga
      781 gcaagccttt agaaaagctg gaacagaatt gttagagcca tatcttagtt ttaaagttta
      841 tgcaccacag gaatatcttt cacgggcata taacgatgct cccaaatatt gtgcaaatat
      901 cgtaaatact caactgaaaa ataatgaggt cattattatt ggagaaattc ctgctcgatg
      961 tattcaagat tatcgcaatg atttaacttt ttttacaaat gggcttagtg tttgtttagc
     1021 agagctaaaa ggatatcagg ttaccactgg cgaacctgtt tgccagaccc gtcgtctaaa
     1081 tagtcggata gataaagtaa gatatatgtt caataaaata acttagtgcg ttttatgttg
     1141 ttatataaat atggtttctt attaaataag atgaaatatt ctttaatata gatttgaatt
     1201 aaagtggaaa ggaggagatt gttattataa actacaagtg gatattgtgt cctatttgtg
     1261 gaaataaaac aagactacga atacgagtgg atactatact taaaaatttc cctttatact
     1321 gccccaaatg taagaacgaa actttaatta atgttcaaaa aatgaatata ataacaatca
     1381 aagagccaga cgccaagacg cagagccgat aatttgagaa atgaaactct catcttatcg
     1441 gctctttttg tttatctgaa ttttactgac tagccttcaa tatttcttgc ttcattgata
     1501 ccttttgcta gtgcttccat taaggatagt tctttgtctg taaagctatc catgtatttt
     1561 tctacctgta accttcgagt gcttttaatc aaattatctg taggtaagaa aaattcatca
     1621 atagatacat ggagtaatga tacaagatca tacagcactt gtgtacttgg gtgttgtcct
     1681 ttgttttcta tatttgttaa ataacgtggg tcaatttcaa tcattgttcc cacttgttca
     1741 cgagtgagac cccgtttcat tcgagcctct ttaatcgcta aaccaacggc tctaaaatcg
     1801 tatttatctt cttttttacg catactaaat cacctctata cattttactg ttcctatata
     1861 accataaaca ggtatagaaa aacatgttat atagtttatg agttcctata gaacactgtg
     1921 gaatttatta atgcgatgaa tccaatttca aatagaaagg tcgctgattt tatttaccta
     1981 tacaacttta taaaatttag tcatactcct agaaagtaga taaaattggc gacttaatgg
     2041 agggaaaagt cgccatgaga aagataatct gtcatctagt tctcttaatt aagttcttat
     2101 caaaaaaact tatccacctg tactggatag atatgaccat caatgtggtt ttgcagatta
     2161 catagataga aaaatcaaag catatgaaca gagaaaacaa tctgtttatg tgcttttttg
     2221 gtcacttaat aaatatcttt taggatttct cttcatttat gcaacaaatc ccccgttcgt
     2281 gtcggggttt agtatgaagg gagataaaga agcacatgcc actccctttc acgacagaaa
     2341 gggggtgaaa agcatgaaac catcttcttt tcaagaaacc atagaaaatc agtttgacta
     2401 tatctgcaaa caagttattg aagatgaacg aaaagattac ttcaaacatc tagacagact
     2461 atcagcaaaa gaagtctcgt tttctgatat agataactac cttgtcaact ctttttctac
     2521 tgttgatacg tacgtcactg atttccaact atttaaatta tttggattaa gaatcaatgt
     2581 tgaaagtgat ttattaagtg aagccctgcg tcatctttca gagaaaaaac ggaacattat
     2641 tttacttcat tactttatgg atatgagtga tgtggagatt gcggagttac taaatttgaa
     2701 tcgttcgact gtttatcgtc atcgaataag tggactagcc atgattaaag aattcatgaa
     2761 ggagtgtaaa gaatgaaaac agcttatcca agggttccct tccccctcat tgtaaaggcg
     2821 acagatggcg acgtggaagc aattaatcag attgttaaac attatcgagg gtatacctca
     2881 aaacgttcat tacgtcgcat gacagacgaa tatggaaatt ctcatatggt tattgatgaa
     2941 accctacgtg gcagaatgga aacgaaactc atctctaaga ttttatcgtt cgaaattaaa
     3001 tagtcccatt ttttcgtgga agcgtgtcga atcgtatcca cgcttcccga tttgggaggt
     3061 ttgttattcc atcaaagcat attgtgtgtt cagtaggttt tgataggcta acaagccaaa
     3121 cgttctttga caaccaaata aaggcaatcg aatacgtttc ggtaagaaaa gagctgatga
     3181 actaacacgc catgacctac aaaattagtg agcgattcat gttagtaatt cgaaagtaat
     3241 ctttagcagg attgctgcca tgactttctt atcgtgataa tgatactccc gtatagccat
     3301 agtccgagcg ttagaagcgt cacaggcaat gggtacggct acatgagaac catgcagaag
     3361 tgaaattcct gtgagctttg ctagagctgt tcgattgcta agatattctt ctcttgtcag
     3421 tgtattatta taaaactatt tgatataatt ataggtgaat attggagttt tcctagccta
     3481 taattgggaa aggagtaata atgataacgg tcacaaaaaa ggatttagtt gagctaggat
     3541 atggaacttc tttttcagca gacatcataa aaaaagcaaa agaattgatg attacaaaag
     3601 ggcatatcta ctatcaatct cgaaagctag atagagtccc tcgtgaagct gttgaagaaa
     3661 ttctaggaat tcggttttca aatgaaaaca ggtctgagta gtttttgcac agtaaggagt
     3721 aaaaactatg gttaaagacc caataaaaaa agcaaagaat ggaacctatt attttcgtgc
     3781 aaatctaggt tatgattcta aagggaaaaa aattcaaaaa tatcgtagtg gattcactac
     3841 taaaaaagaa gcaagagaag aatattcgaa actgctattg atgaaaccag aagaattaag
     3901 tgagaataag gataaaatga cgtttaaaca ttatatcttt gaaattttcc ttccatggta
     3961 taagacacag gtaaagctca gaacatatga aaatcgctta ccaactatta agaagcattt
     4021 ctcttatttt gataaaatgg ctgtttccga tattgagcct attgatgtgc aaaattggca
     4081 actaaaatta tcaaagaagt gtaagtcttc ttatgtaaga gctgttcaag gactattttc
     4141 agttgctatg gatcgagcaa ttgtccttgg gataaccacc acaaatccat ctaagattat
     4201 tggcaatgtg aaaaagcaaa agtctaaaat tgaattttgg acaaaagaag aatttgaaaa
     4261 agtaatctct ctaatttaca aagaagatta ctatcaacac tttttattta tctctctgtg
     4321 gtttttattt atgactggaa tgcgaattgg tgaagctact gccattcaat gggaagatat
     4381 tgattttgac tcaggagtac taaccatcaa caaaacactc tattacaaga atcaatcgaa
     4441 ttatagattt gttgaaccaa aaacaaaggc gagcgttcgt catattgtat tagataaatg
     4501 cacattaact tatttatccg aatggaagga agtacaacaa aatctaattc agacagactt
     4561 tgtaatgagc tataacggta ttcctaccca aaaacataca ctatcatatg ctattgaacg
     4621 ctatgctaag ctggctggta tccatcgaat cagaatccat gggttacggc attctcatgc
     4681 ttccttgtta atcagtatgg gagaaaatcc acttatcatt aaagatcgtc taggacacga
     4741 agatattgag acgactttag gaacgtatgg acatctttat cctaacagta atttcgaggt
     4801 agctaacaaa ttaaatggag ccatttcctt taaagaagca aaacaaaatt atgatacttc
     4861 accaaagaat caatttactg tagactactt gcgaaacaaa gaaatgaaaa aagtgcaata
     4921 aaagtgcagt aaaaagataa gaagagccag aaacccttgg tataagagga tttctagctc
     4981 tcctacaact attcccactc cactgttgct ggtggtttag aagtgatgtc atacacgata
     5041 cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa gacttcccat
     5101 gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg gataccgact
     5161 gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc tggaagaaca
     5221 gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc acgcaagata
     5281 gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag aacacgaata
     5341 ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat gcctaactga
     5401 gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat caattcaaat
     5461 tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg tgctgtttct
     5521 gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga gacgccttct
     5581 tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt cccaatgatt
     5641 ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg atctcttgca
     5701 tcgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac ttgttccgct
     5761 tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc accgatcgct
     5821 ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc aagcaagact
     5881 tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa gttatccatt
     5941 gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa ttcgattccg
     6001 tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg tgttgtgtct
     6061 tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc ttcaggaact
     6121 ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt ttctttaaac
     6181 aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg gtttttagca
     6241 ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca aatccctaat
     6301 acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc atcatataca
     6361 ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac ttcttctgca
     6421 gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg tcttgtgatc
     6481 aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt cgtcatatcg
     6541 gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa atagtcaaac
     6601 tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat tttcagtttt
     6661 atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta aatttttcaa
     6721 attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat ctccgatagc
     6781 aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg caccaaaacg
     6841 atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag aaaaatcaaa
     6901 aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga tcccctcaac
     6961 gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat catacacatc
     7021 atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt tcaagaaatc
     7081 gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga ttccacgctc
     7141 ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa cgtttcttcg
     7201 tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt ttccaacggc
     7261 tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat gaagaaataa
     7321 acctttactc aagaacaaag attcaaattc tttcatataa agataaatca gatgagcaag
     7381 aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac ttttgatatt
     7441 atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc gccattcttc
     7501 tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg ttctcccatc
     7561 aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga gattgccgat
     7621 ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact tgcgcacgga
     7681 tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga catgacaaat
     7741 cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc ttttgagtta
     7801 aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga aacagtggat
     7861 tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga tgaaggaaag
     7921 atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt tgcgacacag
     7981 cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca aggaaacgca
     8041 aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga agcagttcat
     8101 gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa aaaagttgta
     8161 catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact gacaattgtg
     8221 atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac atttggtaat
     8281 gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa agaataacta
     8341 gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg ggtacgtatc
     8401 agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt caaaaagaaa
     8461 tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga acttgtatag
     8521 aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt tccttttttc
     8581 tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga agggagccaa
     8641 taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag taacaactgt
     8701 ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa aaaaagtgtt
     8761 gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg tgatcaatcg
     8821 acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct tctccaaact
     8881 gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt tatgcaattc
     8941 tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc gctcagtgga
     9001 cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag acagcggttt
     9061 ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg ataacggacg
     9121 gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta ttgaaaatgg
     9181 ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag aagaacgttt
     9241 caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga aatttgttga
     9301 acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac tcaaacataa
     9361 agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct atcaggcgat
     9421 cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg atggattaaa
     9481 gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt ttgatattgg
     9541 attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa aagttccgaa
     9601 caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga caaagggcta
     9661 actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct ttttttattt
     9721 ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat aataccgaat
     9781 gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa gagggatgga
     9841 aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa tggaaagatt
     9901 ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat gacaaatgaa
     9961 gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga tttgagttct
    10021 cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac gaccacattg
    10081 attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc tggacgcggt
    10141 ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt tcagatcgag
    10201 ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt aattggtcaa
    10261 tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt cacagcgacc
    10321 gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc ggcaggagca
    10381 gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa catcgaagat
    10441 gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg agaaacagtg
    10501 gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag tctagaagtt
    10561 gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga aatgtgctac
    10621 gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga tgaagcaaga
    10681 acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa agaaatgatc
    10741 caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt gactgcacgc
    10801 tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt ggcaaatgaa
    10861 ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga agatattgat
    10921 cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa aggtgagtca
    10981 ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt attatataaa
    11041 aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat catcacagaa
    11101 taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat ggaactaaac
    11161 cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt gatgcaaatt
    11221 atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc atgggttgcg
    11281 ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat cgggtttcct
    11341 ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat caataacggt
    11401 gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga tgatcaacaa
    11461 gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct tgtcaaagtt
    11521 attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga aatagcaaaa
    11581 gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg agcaaaggta
    11641 gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa agcttccggc
    11701 ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac aagaatcgga
    11761 acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta attttttcaa
    11821 aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt gaagcgttag
    11881 ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta acggaatcag
    11941 gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg aattgtgccg
    12001 agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg catctagtcg
    12061 tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa gtaatggcag
    12121 aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg acgaaggcaa
    12181 cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa ctcatgttca
    12241 ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta cagaaatgaa
    12301 aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat ttttaggtgc
    12361 atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag acacaacaac
    12421 tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc agtcagcttg
    12481 ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg atggcttcca
    12541 atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag cattgaatgc
    12601 tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc aagaacaagc
    12661 aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg gattagataa
    12721 cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg ttgcagcagc
    12781 ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg aagtaatcga
    12841 tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat taggaaaaga
    12901 aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag gacgttcaat
    12961 tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag gcggtacagg
    13021 taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa aagtttgggt
    13081 tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg gcgaaaagaa
    13141 aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag atttggcaca
    13201 aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat tgaaagaaga
    13261 cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag ttgatgaagc
    13321 aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag aaaactaaaa
    13381 ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat tagaacaaga
    13441 attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac tccctgaagt
    13501 aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat cgttgcccta
    13561 gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc atagaaatgc
    13621 agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat ttacaagtaa
    13681 aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct acattgatga
    13741 acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt gggaaagaag
    13801 taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc catcagcact
    13861 ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc gagcctagtc
    13921 gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt tctgaacagt
    13981 atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga atggaacaac
    14041 gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt gatgagccga
    14101 cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag gaattagtca
    14161 aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa gcagtagctg
    14221 accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt aaagacgtaa
    14281 cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa acgatgaaaa
    14341 aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg aaagaaaatc
    14401 gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa gtacttggta
    14461 tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact ggtttgcgaa
    14521 aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa aaaccccgaa
    14581 aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac gggttagtcc
    14641 tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa ccttacagta
    14701 aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg atcgaagaat
    14761 atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc ggaaatcagc
    14821 aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt gttgcaaatc
    14881 caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg atcgaacaaa
    14941 gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt ttaaatgttt
    15001 cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat ccgaaagaaa
    15061 catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa gcacgaaaag
    15121 agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg taatttatta
    15181 gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat ggctgctttt
    15241 ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg ttcgcaaaga
    15301 gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt aggtttttct
    15361 gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact ttgcgggtgg
    15421 atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt tttgcttccg
    15481 ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc aggtttactt
    15541 cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta tatcttatta
    15601 tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc aagtttggac
    15661 tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac acaagctttt
    15721 ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat tttcgtttgg
    15781 ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt gaatcctttt
    15841 gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat ggtcatttct
    15901 ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca aaacttcttt
    15961 gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt gcttggttct
    16021 ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa gatcggtgga
    16081 cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc gattgctttg
    16141 attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa agtcttccaa
    16201 gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa tcaaaaccat
    16261 gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt acttctacac
    16321 tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct gaacgaagcg
    16381 ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt tccatcgttt
    16441 tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct attttagtgg
    16501 gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc agtttgcgtg
    16561 ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta ggtgttttct
    16621 tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat ctggggtatt
    16681 tttctttccc aggattagca gatattccgg tcattggacc aattttcttt aaaaatacat
    16741 tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc atcttcaaga
    16801 cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc gacacattag
    16861 gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg ttaggcggta
    16921 tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt acgatcgttg
    16981 gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct ttaggggcaa
    17041 tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc gcacaattac
    17101 cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg ctgacaatca
    17161 ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg aagaactata
    17221 tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt gtttttcgtc
    17281 tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa tcatgtttaa
    17341 acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag atgccgataa
    17401 gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag gattgaacgt
    17461 tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg tggaatcagt
    17521 gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga aagatacgat
    17581 gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg tgtttcctaa
    17641 tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta aagtcgtatg
    17701 taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc aaatgaaaac
    17761 aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg cacatgaaga
    17821 aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata taacaaaaga
    17881 tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac caggagactt
    17941 cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca cagtccttga
    18001 ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg atattttcaa
    18061 cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg ttgaccagct
    18121 gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag tagattttga
    18181 tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg aagaatttga
    18241 cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa tgattacagc
    18301 cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat atgtaccgtt
    18361 attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat attattcaga
    18421 tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg gcgaaagctt
    18481 cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa gttaagcgag
    18541 atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat tgattttggt
    18601 ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc tgtaatgatt
    18661 tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc aggaaagcta
    18721 gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt ccactactat
    18781 gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg actaggggcc
    18841 cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt accaggagat
    18901 ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat cggtccgaat
    18961 gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc tgactatcaa
    19021 gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg cgtatatatg
    19081 ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg gacaatagga
    19141 gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca cagcggatta
    19201 aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc gaatctgagc
    19261 catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc attggttaaa
    19321 gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata ttgaagcgaa
    19381 agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag ctaaatatat
    19441 tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta tgttaggctt
    19501 taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg gaatgccatc
    19561 agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat tgattcgagt
    19621 aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac tagcacaagg
    19681 tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt ttcctccaat
    19741 cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg gctatacggt
    19801 tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga cagagacgtt
    19861 ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc tttattactt
    19921 aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc atttgatcac
    19981 cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga tcgaagtagg
    20041 attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc attaagtaat
    20101 aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact tattattgca
    20161 aatgtaagct tttgaacacc gtttaccaag aggttgtgaa
//
LOCUS       DACCQI010000056        20006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCQI010000056 DACCQI010000000
VERSION     DACCQI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.057"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..410
                     /gene="dltD"
                     /locus_tag="IVO76_001566"
     CDS             <1..410
                     /gene="dltD"
                     /locus_tag="IVO76_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7429534.1"
                     /translation="NWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGL
                     SQEMLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPF
                     LEENHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            551..1633
                     /locus_tag="IVO76_001567"
     CDS             551..1633
                     /locus_tag="IVO76_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7429535.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1633..2436
                     /locus_tag="IVO76_001568"
     CDS             1633..2436
                     /locus_tag="IVO76_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7429536.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2506..3246
                     /locus_tag="IVO76_001569"
     CDS             2506..3246
                     /locus_tag="IVO76_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7429537.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            3257..4261
                     /locus_tag="IVO76_001570"
     CDS             3257..4261
                     /locus_tag="IVO76_001570"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7429538.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(4306..6150)
                     /locus_tag="IVO76_001571"
     CDS             complement(4306..6150)
                     /locus_tag="IVO76_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429539.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            6362..6880
                     /locus_tag="IVO76_001572"
     CDS             6362..6880
                     /locus_tag="IVO76_001572"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7429540.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            7008..7691
                     /locus_tag="IVO76_001573"
     CDS             7008..7691
                     /locus_tag="IVO76_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7429541.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            8000..8073
                     /locus_tag="IVO76_001574"
     tRNA            8000..8073
                     /locus_tag="IVO76_001574"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8034..8036,aa:Arg,seq:tct)
     gene            8232..8591
                     /locus_tag="IVO76_001575"
     CDS             8232..8591
                     /locus_tag="IVO76_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP7429542.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            8714..10345
                     /locus_tag="IVO76_001576"
     CDS             8714..10345
                     /locus_tag="IVO76_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7429543.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(10407..11048)
                     /locus_tag="IVO76_001577"
     CDS             complement(10407..11048)
                     /locus_tag="IVO76_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7429544.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            11330..11638
                     /gene="rplU"
                     /locus_tag="IVO76_001578"
     CDS             11330..11638
                     /gene="rplU"
                     /locus_tag="IVO76_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7429545.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            11665..12009
                     /locus_tag="IVO76_001579"
     CDS             11665..12009
                     /locus_tag="IVO76_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7429546.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            12022..12315
                     /gene="rpmA"
                     /locus_tag="IVO76_001580"
     CDS             12022..12315
                     /gene="rpmA"
                     /locus_tag="IVO76_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7429547.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            12722..15556
                     /locus_tag="IVO76_001581"
     CDS             12722..15556
                     /locus_tag="IVO76_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7429548.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            15747..16238
                     /locus_tag="IVO76_001582"
     CDS             15747..16238
                     /locus_tag="IVO76_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7429549.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            16341..17330
                     /gene="manX"
                     /locus_tag="IVO76_001583"
     CDS             16341..17330
                     /gene="manX"
                     /locus_tag="IVO76_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7429550.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            17363..18166
                     /locus_tag="IVO76_001584"
     CDS             17363..18166
                     /locus_tag="IVO76_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7429551.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            18187..19101
                     /locus_tag="IVO76_001585"
     CDS             18187..19101
                     /locus_tag="IVO76_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7429552.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            19184..19558
                     /locus_tag="IVO76_001586"
     CDS             19184..19558
                     /locus_tag="IVO76_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7429553.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            19697..19942
                     /locus_tag="IVO76_001587"
     CDS             19697..19942
                     /locus_tag="IVO76_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7429554.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         6436 a         3305 c         4231 g         6034 t
ORIGIN      
        1 aaaattggga ttatcgtttt tcgccagaat tttctgattt tcagctggtc ttggatcaat
       61 tagcaaaaaa ccataacgaa gtattgttca tcatcccacc ggttaacgag aaatggagcg
      121 attataccgg actatctcaa gaaatgttac aaggatttgc caaaaagatc aaatttcaat
      181 taaatagcca aggattcaat cgaatagctg attttgtcaa tcaagcgggt accaactatt
      241 ttatggaaga cacgatccat ctaggctgga aaggctggtt ggctgcagat caacagatca
      301 gaccgttttt agaagaaaat catattactg ctagtaagta tcatcttgac gatgcttttt
      361 ttagcaaatc ttggcaacat caaataccag ataaactaca attaaaataa agaaaacagg
      421 cgagtgttgc tcgcttgttt tctttatttt tgaatattct gaataaaaga taaaaatacc
      481 aagtttgcct gaacaaaaat cataagcaat ggtataatca caatatgaat atagaaatgg
      541 gggaaaacat ttggatacaa aagaagaaca gctacttatt gatttaactt ctgcaaaagg
      601 agtacctgga aatgaagaag aggtaagaga agtattcaga gaatatgcca aaccattcgc
      661 tgatgatatt ttttatgacg gtttaggcag tgtgattgcc aaacatggtg ctggcggagg
      721 ccccaaggtc tttatttctg gacatatgga cgaagttggt tttatggtaa caaaaataac
      781 cgaaaaagga ttcttggaat tccaaactct tggaggttgg tgggggcaag tcatgcttgc
      841 tcagcaagtt gaaatcaaaa cacgtgaagg gaaaatcgtc cacggtgtga ttggttccaa
      901 accaccacat gttttaacac ctcaagtaag aaataaacct tatgaaataa aggatatgtt
      961 cattgatatt ggcgcatcaa gccaagaaga agccaaagag tggggcatac gtcctggaga
     1021 tatggtcact ccatatattg aatacaaacg aatgaatggc agtaaatatt tactggcaaa
     1081 agcatgggat aatcgtatcg ggacagctgt ttctctgcga gtattagaga atctatctaa
     1141 agaagcacac ccaaatgttt tattcgcagg aagcgatgtg caagaagaag taggcttgcg
     1201 aggtgctcga acaagtacac accttgtaaa tcccgatatt gcttttgctt tagatactgg
     1261 tacagcagga gacactccag gaatgacacc aaaagaagca gattcgattt taggaaaagg
     1321 cccacagatt ttgatttttg atgcatctat gatcccgcat aaaaaattat tgaattttgt
     1381 tattgatgta gcagaggaaa tggatattcc attccaatat acagtgattt ctggcggagg
     1441 aactgatgca ggacaaatgc atttgacaag agatggtgtt ccgtctgtag ccattaccgt
     1501 tcctgttcgt tatctgcatt cccatacatc agtgattcat gaagatgatt atttgaatac
     1561 agtgaaacta gtcacagaat tagtgaaacg gcttgatagt gaaacagttg aaaaaattcg
     1621 gagttatgaa taatgaataa tagaatacct atttctgaat atcggaaaaa agcacgagtt
     1681 tctttggaaa atcaatgggg aatcaatact tggcttgttt tcttatctat aatatttacg
     1741 agcgtcatcc agaacttttt tgacggtttc agtccttttc cggaagaaag catgcagcag
     1801 cacttattga gttttctagt cgataattta cttttattcg cattcaacta tgcattgttt
     1861 tacattgcgc tagtagttgt acggggtggc agaccaaaaa gtaacctttt attttcagtt
     1921 ttccaaagaa agtattttgg ttcgatactt ggcatcaatt tgatcaattc agtcatcaac
     1981 tggctactca acagtttaat ctttctgccc atgtttttaa tcagcggagt gagtacttat
     2041 atgcagctgc ttttcagttc aaatgcggta cctacgggtg tcatgccaga agattttctt
     2101 gatatcagct ttgttttatc gatgataggg atgatttttg tttcagcact catcatgagt
     2161 attgttggcg gtttattcca atttgctgca tggacaaagt tcgactatcc tgatttgtcg
     2221 gttattcaat gtttgaaata tggctggttt ttattgaaag atagaattgg tacgtatatt
     2281 ttgttgcagc tgtcttttat cggctggtat ttgctaggag caatcgcttt gttcatcggt
     2341 cttttgtggg tcattgttta tgtgaatgtg tcagttgcag aattttatga acaagcacgg
     2401 attgaaaaag gaggtccaga cgcctatttt acttagcatg taagcagttt attcatcaaa
     2461 aaaataactt ttgtggtagg ataaatcttt gagggggaat gtacgatgaa attaactgtt
     2521 ttaggatgct taggagcgta tccttataaa aatcaaggga caacaggtta tttgcttcaa
     2581 tctgacgggt tcaatttgtt gatcgatgca ggtagtgcta cgctgatcaa attacaagaa
     2641 caccttgatc cacttgatct agatgctgtg atccttagcc attatcatca tgatcatatt
     2701 gctgaccttg gtgtattaca gtattactgg caacttcatc ctaagagaaa tccggagaca
     2761 ttgctttcaa tttatggaca cacaaaagat tccattcact ttgaagaatt gacgttggaa
     2821 aatgtctcaa aaggacatcc ttattttgaa gctgaagaat taaaaattgg tccttttctg
     2881 attactttta tggaaacaat ccatccagta gtatgttacg ccatgcgtat cgtagaagaa
     2941 acaacgggga aagtattcgt atttacaggt gattcaggtt atttggaaag tttcgctgat
     3001 tttgcgaaag atgctgattt gtttctagca gatacctatc tttttgaagg aaatgaacat
     3061 catcatgccc atttcacttc aaaagaagct ggagaacttg cgaaatcagc taacgtaaag
     3121 aaacttgtac ttacacacct acctcagttt ggtgatttag aacaattgaa aaatgaagcg
     3181 caaaaggcag caggcaaagg gattgatata gaacttgctg cagttgacaa agtgtttaac
     3241 atttaggagg aagaacatga tttttgtacc aaatgaaaac cacgacccaa gaatcaattt
     3301 agcaattgaa caatatttgc tgcaagaaat gcctttagat gaacctattt tgctctttta
     3361 tatcaatgaa ccttcaatca tcatcggcag aaaccaaaat acgcttgaag aaatcaatcg
     3421 agattatgtg gaagaaaatg gcattcatgt tgtccgtcgc ttaagtggag gcggagctgt
     3481 ctaccatgat tttggaaacc tgaactttag ttttatcatg cctgatgatg gggattcatt
     3541 ccgtgacttt gcgaaagtaa cacagccaat catccaagca cttcatgaat tgggtgttgc
     3601 tggggcggaa ttgaaaggcc gaaatgatct agtcattgat ggcatgaaat tttctggcaa
     3661 cgcgatgtat gctaccaacg gtcggatgtt tgctcatgga acaattatgt tcgactctga
     3721 tatcaatgaa gtagtcaatg cattaaaagt aaaaaaagac aaaatcgaat caaaaggaat
     3781 caaatcgatc cgttctcgtg taacgaatat caaaccattt cttccagaag aaaagcaaaa
     3841 aatgacaaca gaagaatttc gtcaagaaat cttgttgaaa attttcggcg tagagtcagt
     3901 agaagaagta aaaacgtacg aattgactga tgaagattgg aaaaaaatca atcgaatctc
     3961 tgaggaatat tatcgaaatt gggattggaa ttacgggaag tctccagatt tcaatttctc
     4021 tcgccaaaaa cgtttttcta tcggttcgat tgaagtccat ttgaatgtct cagaaggaat
     4081 gatcaaagaa gcaaaaatct ttggcgattt cttcggacta ggagatatct ctgacgtgga
     4141 aaaaaaattg gttggacaga aatatgataa agcatctcta tcaaaaatag tcagccaaat
     4201 tgatgtgaag aaatattttg gtgcgataga accagaagaa ttgcttacct tactttattg
     4261 aaaataaaaa atagcggaat ccacatatat ggattccgct attttttata caggattcgg
     4321 gtaatttcga gagagttgaa cagtgatccg atcaatgaaa gtatcttttt cctgcgttag
     4381 tgttcctgtg taacgatccg ctacgtcttt catcatcatt aagtcaaaac gttcctgatc
     4441 atctaattct tctgtgatgc ctcgactaaa taggtttgta tcgtaatgtg cactatgttc
     4501 gcaacaaatg acgaaatgtt cctcttgttc gtgtatcgac aaataaattt ttcgatacag
     4561 tggattttcg atggcatgag tttctctaaa ggaatgctct aaagcgtgga tcaaaagttg
     4621 agccagatcg tcatcgttga taggaagttc ttcgggaaga tctgcttgga aggttatctg
     4681 aatattccgt ttggcagcta acttctggta acgcgctaag ataagatttg tcacctggtg
     4741 gttgcaaaaa gaacacagca tttcgaaatt ttttgcttct tctaatatct tttccacata
     4801 gtttttagct tttgtttggt ccgtatgcat taaatcttgc agattctcca tctcttggac
     4861 aaattccagg cgaagtgtat ttacttcttg gatatgctgt cgaagctgct cataatgttg
     4921 ctccaacaaa tcatttttga tttttaagaa atctacttgt ctccgatctt tgtcggtatt
     4981 taagatgtga tcgattagat tgtagctgat aataacgatt agaatgataa tatataaaat
     5041 cgtagaccaa ttgatcgttg tttgatagat acctaatgcc gtttggatgt aaatcaagca
     5101 atggataatg gctaccaaga cgatccaagg tgtacaagct acataaaaac ggttattctt
     5161 atatgcttcg ccaatgcatg caagtgaagt gactaaagtg cttataacac tagcaatatt
     5221 agcaatcggc attaattcag gaagttctgc aggagtaaaa gttgcataaa gcagaacgcc
     5281 gataaaaatc aaaagttgga acgtacaaaa aattccgtac cattttttat agtaaaacat
     5341 tcggctccaa tagtagcaga tgatgaaagc tgacgttaag ataataaata aatgagataa
     5401 aaaagaatga acgaatggtt caaaaagaat accacttaaa atatcttctg atacagcttc
     5461 aaatcctaat gccaaagcaa agcaacttaa gagaataagt gagtattcaa gacgtttttg
     5521 tttatacatc aagtagatcg tatagaaaaa ggtccctata gcgataaaaa cagcgattat
     5581 catcgtcaat acttgcggca ctgattgtga ataaataaaa ctaatcaatg gtccagttgt
     5641 tcctagataa actttcggtg gcagtccagc atagttctga taaggagatg agactgtaag
     5701 ggtcaatttt tttccgatat aatttgctgg taaatctatc acagccaatg agagtccggg
     5761 attgctttct tcctgataat ctttccgttg gtataaaatc ttttcgccta gatatacagt
     5821 aatccattga tggtttcctt tgattagtaa atttgcttcg tccaacggtt tttcaagtgt
     5881 cgtttccata atgaatgttt cgtctttatc cacggtagga atcgtacgag tgtacttgct
     5941 gtcaaactta aaatcaggat catcttttgt atagaaaacc cacgaatctt ttatttcttt
     6001 catttgattg ctgtccgcag gagaaaaaat aaaataaaag aaataagcaa ttcctgttaa
     6061 aaataacaat gataatataa tgattatgct ccactttata cgtaaataaa aagattcttt
     6121 tttattatat atgttattaa aaaaagacat aaaggtattc ctttctaaaa cttgtaatgt
     6181 tagggtaaac gttttttaaa ctaacgtcaa ttatttataa taaaaaatga gataaagatg
     6241 aggaaataga attagaaaca tataattatt aaagggaaac atttttacag ctataatttg
     6301 aaaaaaattt atcgttatta tatgattaag aaggattgaa taaagaaaga agttgaaaac
     6361 gatacctgat tacaaaaaaa taacttttat aagttctata acgttgatta tatttttaat
     6421 tcttattctt ctcgtcgtct tggttccccg aatttttggt ttgactcctt atattattga
     6481 ggacaaagaa atgagtccat attatgcaca aggaaatctg atatatgtac gaccgaaaaa
     6541 tacaaatgag attttagtcg gagatattat tacatattat gaaaattcag gaaaaaaaat
     6601 ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa gatttttata caaaagctga
     6661 tacaaagact tatattgagc ctggagtagt aaaaaagaga aatattatcg gtagtcccat
     6721 ctttcagatt ccatatattg gactagtcct ttcaaaaacg gcatttgcat ggattcaggg
     6781 gttattcttt acgattgctt tttgcctaac aggaatcaca gcatggaata cctttgtaga
     6841 gttgaaaaag aagcggagac gaacacagaa atttttgtaa gaatgttaaa aacataaatg
     6901 tattccagag atccaacgac tcttctgaac atgaaacacg agaaaacact catgcagctt
     6961 gtagatttag tcagttgttc gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa
     7021 tgatgcatcg tcctgtagtc aaagaagaac tattggattt tatgcgtacc cagcaaaaaa
     7081 aattaccggg tgagctaggc aaactggaag aagaagctca tgtagcagaa gtaccagtta
     7141 ttccccatga aacagttgta tttttgaaat ttttacttgg acagcttcag cctgaacgaa
     7201 tcttggagat cggagcagct atcggttttt catcaagtgt catggccacg acaatcggag
     7261 aaaacgctca tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa
     7321 actatgaacg tctaggactg acagataaag tgactttgct tgaaggtcaa gcagcagata
     7381 tcttgccgga gttgtctggt ccgtatgact ttatttttat ggatagtgca aaatcaaaat
     7441 atattgaatt tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag
     7501 atgacatctt ccaaggagga acgatcttgc tcccagatga agagattccc cgtggcaaac
     7561 gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat cccgatttga
     7621 catcgacaat cttgccatta ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa
     7681 tcgagttgtg atacatgatt atggcagcct gtagataatc gattgaatcg agggctcaat
     7741 tcgaacttga tgtaaaagtt tctatatttg taagtcaatt aaaggataca ggaaatctaa
     7801 ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt caattgataa ccttagaaca
     7861 gctgaaggag aatgtctcgt tttaaaagat tgatttagta gttgggccaa aataattcat
     7921 tatttgctaa ttaaataact atttcgtatt gacagaagca atttaattat ctataattat
     7981 tgatgttccg ctgattcttg tcatagtagc tcagctggat agagcatccg ccttctaagc
     8041 ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa accgttagaa actgaggttt
     8101 ctaacggttt ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc aaataaaata
     8161 aaaaaacatc cttatcgagt acaattataa ggagcaaaaa aataaaactc atcagaaaag
     8221 aggaagaaga tatgacgata ctatctagaa ttttagtgac tctagttgca attgaatttt
     8281 tttatatcat gtatatcgaa acggtgagaa ctgattctga cacgacaagt agggttttta
     8341 aaatgagtaa agaagagtta agcagaaaat ctgttcaaac attgtttaag aaccaaggag
     8401 tgtacaatgg actattagga gtgggactac tttatggagc ttatctttct tccgcatcta
     8461 aagaaatcac aagtatgcta ttaatatcta ttttttttgt tgcattatat ggcagtttga
     8521 ctagcgatcg ttggatttta ctcaaacaag gtgggctagc tctgttggca ttgctttcta
     8581 ttttcttata aatatagaaa aaataagaat ttatctaaca ggaaactttg tgaaaaatat
     8641 tactttatct catgaaatat gatattctaa tgaatggtat tttagaaaat ttcatgaaaa
     8701 aggagttaga taggtgttca aaaattatgt ttcctcacta aaaaaagaat tttcaggtta
     8761 tgatctttca agatttcaga aagatctttt ggctgggatc acagtagctg ctgtagcatt
     8821 gcctttagcc ttggcttttg gggtatcaag cggagctgat gcggccgcag gtttgattac
     8881 ggctatcatt gccggtatcg ttattggcgg tttatcaggc ggtttttatc aaatatcagg
     8941 tccgacaggt gcgatggcag ctatcttgat gtctattgcg gcaacaaagg gaatgcaagg
     9001 tgtattgctt gctacttttt tagcgggtct atttttactt gcagcaggac ttttccgatt
     9061 aggaacactg acttctttta tcccagcacc cgtaattacg gggtttactt caggtattgc
     9121 tatcattatt gcattaggtc agatcgataa tttatttggt gttcactcgg aaggtacaaa
     9181 cgttattgaa aaactaagta gttatcaaac gataggattt ccgataaaca cagcctcttt
     9241 gatcatggga gggctagtca ttttagggat gtttatctat ccaaaaaaat gggcgaaacg
     9301 aatgcctagt tcgctgctgg ctatcatttt ggcaacagca gttatgctat tgttccattt
     9361 accagtagca actgtgggta agattccaca aacattgatc agtagtaatc gtttgaatat
     9421 gggagatttt agcttttctg ccttgcaaca agtggcagtt ccagctatta gcattgcttt
     9481 attaggaatg attgaaagct tgctatgtgg tgcttcggct ggccgaatgg caaataagcc
     9541 cttagatagc aatcaagaat taatggcgca aggaattgga aatctgttgc ttcctttctt
     9601 tggcgggatt cctgctactg cggccatcgc ccgtacaagc gtggccatca aatctggtgc
     9661 acaaactaga ttagctggta tgatacatgc agttgtctta ctgctctcca tgcttgtttt
     9721 tgcgccagtg atgtcacaga ttccaatgcc agcacttgca ggtgttttga ttgtgacggc
     9781 ttggcgtatg aatgaatggg aaacgatcaa ggagctattt actaataaat attggtcagc
     9841 agttctattg tttatcctta cgatgatttg tacagttatc tttgatttaa gtattgctat
     9901 cgtgatcggg gtgatcggcg gctgtgtatt ttttgttgtc aaaagcgctg ctatcacgat
     9961 ttcattagaa acgatcgatt ggcaaagaat ggggctgcca gaaacggaaa agctgaataa
    10021 ctggacagta gtgtatatta gtggtccact gttttttatg tcttcagaaa aactcaaaaa
    10081 taaattggaa gaactaaatc atcacgatgg tgtcattttc tccatgcgag gcgtaccaag
    10141 tattgatttg acggcacgtg cagtttttga tgaatttcaa gaaaaagcag agcaaagagg
    10201 acaaacgatt atctatactt ccttgcagcc agaagtagaa aaacaacttt ctcacttgtg
    10261 ggaacataca ttcacgcatc aacatcaaac agttgctcat gcgttgacag ctttacatgc
    10321 gaaatatact gcaattagcc attaaaacaa tgaaaaagaa cagacaagac tgtaaactga
    10381 agattttatg gtcttgtctg ttctttttaa tattgctgta aaagtgaagc tagtacttgc
    10441 cagtcttgat ggataacagg tacaagggag cgattgtaga aaatagctgc tggatgaaaa
    10501 agtggaagaa ttcgatagtt tttttctgac cattcgtagg catccttttg ttctgttaat
    10561 tgtaagacag gccctgtata gagttgacca tgatttttgg taaccgtata ttctttgcca
    10621 agtaaacgtt gaagaccaat gtttccaagt gtcgcaatta attttggctg gaccttcatc
    10681 agttcgtaat ccaatattgg tgcatgcgca aatacttcgg aacgagtagg tgtccgattt
    10741 ggataaacag tttctgactg ttgtgtccgt ttattgatcc gatgagtcac gcgatacggg
    10801 cggctcctta cagcactggt gatatacacc tcttctcgtg ttaagccagt tgaagaaagt
    10861 gcctgcatca attcttttcc agcttgaccg ctaaaaggaa catgcgtatc aatctctgtt
    10921 ttccctgggg cttctcccac gagcatcagt ttaggatgaa gaggtccttg cccagcgata
    10981 aatccttcta caggacggcc gttcattctt aaagaagctt cttgtatcag ttcttttggg
    11041 tattccattt atgctcctcc tttttctttt attataagaa taaaagaaac acaatgcgac
    11101 tattttattt ggtagaactg tgaggataga aaagcagaag tattgcttga atttttgttt
    11161 tactttttga ttttaaattt agttgactaa cggttgctgt tctggtaagc tagtactgtt
    11221 gtaattgtgg actccacagc tacaaccgct cagaacaagt aataagcaca gcatatgctg
    11281 tcacttcgga tggcgagtct aagtatatca gaaggaggtg ctttatagca tgtacgcaat
    11341 tattaaaact ggtggtaaac aagtaaaagt tgaagtggga caagcaatct atgttgaaaa
    11401 acttgacgtt gaagcaggag aaaaagttgt ttttgacgaa gttatcttag taggcggaga
    11461 atctacaaaa gtaggcgctc caactgttgc aggtgcaact gttgaaggta ctgttgaaaa
    11521 acacggcaaa caaaagaaag tcgttacttt caaatacaaa cctaaaaaac attcacaccg
    11581 taaacaaggt catcgtcaac catatacaaa agtggttatc gacgcgatca acgcttaata
    11641 gcgtttgaga aagaaggcct atatatgatc aaaggaacat ttaagcgaaa tgacgcaggt
    11701 cagatcgttt cattcacttt gaccgggcat gctgatgctg gtccttatgg aagtgatatc
    11761 gtttgtgcgg gtgtttctgc gttagcgatc agcactgtca acgggattgc ctcattagcc
    11821 ggttttgagc cgattgttga aatgaatgaa gaagaaggcg gttatcttta taccgaagtc
    11881 acttcaggaa tgacacaaga acaaaacaat attgctgaga ttctattaga aaatctttta
    11941 ttaggattac aatcgataga agctgaaaac tcagaatata tccaaattaa aacaattaat
    12001 gataaatagg aggtgcagac catgttgtta tctatgaatc tacaattatt tgcccacaaa
    12061 aaaggtggcg gttctacatc taacggccgt gactcagaat caaaacgtct aggtgctaaa
    12121 cgtgctgatg gacaaacagt tacaggtgga tcaattcttt accgtcaacg cggtacaaaa
    12181 atctatccag gtgtaaacgt gggtatcggt ggagacgaca ctttatttgc aaaagttgat
    12241 ggcgtagtac gtttcgaacg taaaggccgt aacaaaaaac aagtgtctgt ttatccagta
    12301 gctcaagaag cttaattgaa tcttatgatt aaagaggttg tgacaagact tttgtcacag
    12361 ccttttttgt ttataaaaat aaaagttgta gtggaggcta cgaagtttac tttgtctttt
    12421 tacacttaat atttttttgc caatttggaa tcaggatgtt ttgataacta tcgttttcaa
    12481 ttgatggaag ctatattgtt tgctatcagt gaaataatta tatggtaaac agtgttcttt
    12541 ttcttgtttc cattataaaa atcgtatcgg gatttttcgt tcttttaaat tgaacatttc
    12601 tatctatccg cgtgttatca acaaaacaaa atcaggtaaa tagttgacga aacagaaata
    12661 aatacatagt attatgtttt gaagcctaag tacaatagtc tatagaaaag gagcgatacc
    12721 attggcaaaa cgcattgacc gaatttatat gtatgtcaaa gaaaaaaccg ctcatttaac
    12781 aaaggcagaa tatgatcaag gagtaacaac acaggaaatt gctgaagtgt tagggattca
    12841 gcgaacaaat agcagtaaag atctgaatca gctagtgcgc gaaggaaaac tgataaagac
    12901 agacggacga ccagtacgtt acatatatca aacactcgtt acgcacgtaa agccagttac
    12961 aaaacatgta gtcagttaca aagaaacgcc ggtctctgaa gaaaaaaata cactccaaat
    13021 agatactaag gatatctttg caaaaatcat tggagctaac ggcagcatga aaaattcagt
    13081 ggaacaggca aaagctgcga ttttgtatcc gccaagagga ttaaattgtt tgattacagg
    13141 acctaccgga tcaggaaaga cttattttgc acacgcgatg tatcattttg caaaagcgca
    13201 tcatgtaata gatgaggaaa cggaactgat tgttttcaat tgtgctgatt atgccaataa
    13261 ccctgagctt ttgatgagtc atttatttgg gtacgcaaaa ggtgctttta caggcgcaga
    13321 tgaagaaaaa atggggatta tcgatcaagc ggatggcggt atgctgtttt tggatgagat
    13381 tcaccggttg ccgcctgaag gtcaagaaat gatcttttac tttatggatc atggaactta
    13441 cagtcgtctt ggggaaacaa cgaaatccca tgaagcaaat gtacggatcg taggtgcgac
    13501 cacagaagac ccaggttctt ctctattaga aacttttgtt cggcgtatcc caatcaatat
    13561 caaacttccg gcttttgaaa aacgtcctgc aaatgagaaa gtcgatttag taaaaatcat
    13621 gattgcccac gaagcaaacc gcacgcagcg aaagatttct ttgacagaag atgtagcaaa
    13681 agcattgatc ggcagtgtga cctatggaaa tataggacaa ttgaagtcaa atatccagtt
    13741 agtatgtgca cgaggatttc taaatcatat gcaatcaccc gaaatctcga tcacaatcga
    13801 tgatttgaca gaagggattc gtagcggatt gattcaatta gcaagcaacc gcagtgcaat
    13861 ggcagagctt tctaaaatat tggagccaaa gataactgta gcaccaaatg atacgatcat
    13921 gaaaatccaa tcggactcct atgagttacc ttataatcta tatgatatca tcggagataa
    13981 agcagcttta ctgaagtcgg atggtttgga tcaagaagca atcaatcatt tcatttctac
    14041 agatatcaac atccatctga agtctttcta taaagaccat ggtttttctt ttaatgcaga
    14101 cagcaaatta gcagaatttg tagatccgaa aatcatagaa gtgaccaatc agatttatgt
    14161 aatggtcaaa agatctttac catatgagtt tcagcaaaac tttatttatg ctatgagctt
    14221 gcatatcagt tcttttttga aaaaaatcaa cttaggagaa gaacggcata cgaatgataa
    14281 catccgtgag atggcttttg attttccaga tgaatatgct gttgcgaaag aagtgcgtag
    14341 ttatatcgaa acgtattttc aagtggagat tccagaaagt gaagactatt acttgaccgt
    14401 tctactcgtc tcgttacggg caaatcaagc aagcggcaga atcggtgtag tcgttgcagc
    14461 tcacggaaac agcacagcaa gcagtatggt ccaagttgtc caacagttgt tagatgcaga
    14521 gaacgtacga gcagtagaca tgcctttgga tatggatccg aaaacagcat tggaccgaat
    14581 tgaacgcaat gtacaagaag tagatgaagg aagcggggtc atactcttag tagatatggg
    14641 gtcccttgcg tcgtttaatt cgcaaattca aagagacact ggaattccag tacgtactgt
    14701 tgatatggta acaacttctc tagttttgga aactgtacga aaggtctcag tattagggac
    14761 agatctagat atgttgcatg aatcattgaa aaattttcga ggttacgcag aaatatcttc
    14821 tgtagaatct gttcaggaag caaaacctat tcataagaaa aaagctgttt tggctatctg
    14881 cgcatctggc gaaggtacag cacaaagaat caaagaattg atcgaacgag cggtaaacaa
    14941 gcgtcaagaa acagaattag aagttctagc tttgtcgatt gtggaagtga aagaagaact
    15001 gccagttatc caaaaagaat atcagatcat agctactact gggattactg atttgaatat
    15061 ccaagcccca tttattcctt tagaacgttt tattgatcaa aatatcgaag tcattttgga
    15121 tcagttgctg atggaatcag aattggagga aactgaattc atctccctag atgaagagtc
    15181 agctaaaaat acgtgtgttg agtttatctc tgataacttc atctttatta atggtagtaa
    15241 gttaatcgat ccaatgtggc aattttctac acaaatctct cagacgacgg gaatcggcga
    15301 tgaagaatat ggattcaaaa tcaacctagt gatgcacaca gcaggaatga tcgaacgtat
    15361 tattcggaat gaaccattga ctgtggaaga aaacgaattg acaaatacaa cgaatgatcc
    15421 tctttatagc caattggcag cctcagttgt tctgttggaa gatcagataa aagtaaaagt
    15481 gccgatagaa gaaatgtatt atctgctacg tctagtccat aatcagttag ataaaaaaga
    15541 atacactgtg ccatgaatac acaaaaacga tacactttta tagtgtatcg tttttgtgta
    15601 ttggttaaga atcctttaaa ttcaattagt gtatcgtaaa aaagttggca cgctttttgc
    15661 ttatagtagt gtgtgctagg aggtggttgt tcacacagaa atgatagggg tttctgtgaa
    15721 gtagtgagga agaaggagtt ttgcagatgg atattcgatt ggcacgtata gatgatcgtt
    15781 taatccacgg gcaagtcgca acagtttggt cgaagcaaac aggtgttgaa cgtatcattg
    15841 tgatcagcga tgtagtcgct caagataaat tgagaaagtt tctactaaaa gaagctgcac
    15901 cgccggggat cacagcaaat gtaataaccg tgcagaaaat gattcaaatc tatcatagtg
    15961 gattattatc acgagcaaaa gtgatgcttt tattcaccaa tccccaagat gttgaagcgg
    16021 ttgtccgctg cggtgtacag ttgaagtcgt tgaatatcgg cggtatgagt tttactgaag
    16081 gcaaatcaat gattaccaat tttgtttcag taaatcaaca agatattgat tcctttcgtt
    16141 acttgaacag tcaagaagtg gagttagaga ttcgaaaagt acctgctgac aggaaaatcc
    16201 ttttgatgga actactagcg aaaatcaaaa aggtgtagca agaatggttg ttggaaaagg
    16261 tcaaataatt ctgttgtaat ggttagcaat tattaatatt tttgaatttg ttacaacaaa
    16321 agtaaatagg aggtataaat atggtaggga ttatcctagc aagtcatgga caatttgctg
    16381 aaggaatctt acaatccggt tcaatgatct ttggcgaaca agaaaacgtc aaagccgtga
    16441 ttttgaaacc aagtgaaggt ccagatgact tgagagccaa attggaagaa gcggttgctt
    16501 cttttgataa tcaagacgaa gtgttattct tagttgattt atggggcgga acaccgttca
    16561 atcaatcaaa cacattattc gaagagcaca aagataaatg ggcaatcgtc agcggtttga
    16621 atttaccaat gttgattgaa gcctacgcat cacgcttttc aatggaatct gctcatgaaa
    16681 ttgcagcaca catcattgaa acagcaaaag acggtgtaaa agtaaaaccg gaagaattag
    16741 aaccggcaga agcaccgaaa gcagcagttg aagacgcaca acctaaagga gcacttccag
    16801 aaggaactgt ggtcggagac ggaaaaatca aatatgtatt agcacgtgtt gactcacgtc
    16861 tgttacatgg acaagttgcg acagcttgga caaaagcagt gcaaccaaac cgtattatcg
    16921 ttgtttcaga tgcagtatca aaggatgatc tgcgtaagag actaatcgaa caagctgctc
    16981 ctccaggtgt aaaagcaaat gttattccta ttagcaaaat gatcgaagta gcaaaagacc
    17041 ctcgttttgg aaataccaaa gcattgcttt tatttgaaaa tcctgaagac gtattgacag
    17101 ctgttgaagg tggcgtagac atcaaagaat taaatgttgg gtcaatggcg cattcggtcg
    17161 gtaaagtcgt tgtaagtaaa gtattgtcta tgggacaaga agacgttgaa gcgtttgaga
    17221 agcttgaaca aaaaggtgtg aaattcgatg tacgtaaagt acctaatgat tcacgcgata
    17281 acatggatga tattttgaaa aaagcaaaag ctgaattagc aaaagcgtaa taaataatat
    17341 taaaatttat aggaggctta tcatgtctat tatttcaata attttagtcg tattagttgc
    17401 ctttctagct ggtatggaag gtattcttga tgaatttcaa tttcatcaac cactagtggc
    17461 atgtacattg atcggtttag ttaccggtaa cctcgaagct ggtatcgtac ttggcggtac
    17521 gcttcagatg atcgctttag gttgggcaaa tatcggtgcc gctgtagcac cagatgctgc
    17581 attagcatca gtagcatcag caattatttt agttttaggt ggacaaggtg ttaaaggtgt
    17641 tccttctgcg atcgcgattg ccgttccttt ggctgttgca gggttgttct taacaatgat
    17701 cgtacgtaca atcgctgttc caatcgttca tttgatggat gcggctgctg aagaaggaaa
    17761 catccgtaag gttgaaatgt ggcacatcat tgctgtatgt ttacaagggg ttcgtatcgc
    17821 ggtcccagca gcagcgttgc tatttatccc agcacaaact gtgcaatcat tccttgaatc
    17881 aatgccagca tggttaacag atggtatggc tatcggtggg ggaatggtcg tagctgtagg
    17941 ttacgctttg gtaatcaaca tgatggctac aaaagaagta tggccattct tcgttattgg
    18001 tttcgttgta gctgcaattt ctcaattaac attgattgct ctaggtgctt taggtgttgc
    18061 attagctctt atttacttga acctttctaa aatgggtggt tcatcaaata atggaggcgg
    18121 aagcaactct ggtgaccctt taggcgacat tttgaatgac tattaatctt gaaggaggaa
    18181 aataaaatgg cagaagaaaa aatcaaatta tcaaaaaaag atcgtttagc tgttgcgtgg
    18241 cgctctacct tcattcaagg ttcatggaac tacgaacgta tgcaaaacgg tggctgggca
    18301 ttttctatga ttccagcaat caaaaaatta tacaaaacaa aagaagaacg ttctgcagca
    18361 ttaaaacgtc acttggaatt ctttaataca cacccataca ttgcttctcc aattttaggg
    18421 gttacattgg ctctggaaga agaacgtgcg aatggcgcgc ctgttgatga tgtagcgatt
    18481 caaggggtta aagtaggtat gatgggtcct ttagctggtg ttggtgaccc agtgttctgg
    18541 tttacagtac gtccaatgct aggtgcacta ggtgcttcat tagctatggg aggaaatatc
    18601 cttggaccaa tcatcttctt cttaggttgg aacattattc gttgggcatt catgtggtat
    18661 acacaagaat ttggctacaa agctggttct aaaattactg atgacctttc tggcggattg
    18721 ttacaagacg taacaaaagg tgcttcaatt ttagggatgt tcgtattagc tgcccttgta
    18781 caaagatggg tatcaatcaa attcttacca gttgtatcaa ctgtaaaatt agataaaggt
    18841 gcttacatcg aatgggataa attgccttca ggtggtgaag gaatcaagag tgcatttgaa
    18901 caagttaata atggcttagc cctttctcct gaaaaggtaa caacattaca agacaactta
    18961 gaccaattga ttcctggttt agctgcatta ggattaacat tgttatgtat gtggttgctt
    19021 aagaaaaagg tttctccaat cgttatcatc cttggtttat tcgtagtcgg tgttgttgga
    19081 cacgtcatcg gacttttata atccataaaa atatagctcg ggcgttgtcc cgagcttttt
    19141 ttatatagta tactgttcat aaacaaatat ctggagggag aaaatggttc aatcaatcaa
    19201 tacaaaagta gacttggtaa ttgatgctac ttcgtttacg ggtctgacag attacgggca
    19261 aatcatgata ggtgataatg gttttgagtt ttaccatgcg cgagatagac gcaaatatat
    19321 ccaaattcca tgggaagaag tggattatgt actagcatca gtcatgctaa agggaaaatg
    19381 gattcctcga tatgccattc aaacgaaaaa aaatggtaca tacacatttt cttccaaaga
    19441 acctaaaaaa gtgcttcgtg cgatcagaga atatgtagat gaagatagaa tggtacaatc
    19501 attgagtttc tttgatgtag tgaaacgtgg tattcgtagt atgtttaaac gcaagtaaaa
    19561 aataaagaaa acttctaaga ggaggttttc tttatttttt tactttgcag aagaaaatat
    19621 gaaatagcaa aaccaattat ttgtcccctt taaatcccat atgctaaaat tgattagcaa
    19681 agaaggaggt tatattatga gttggttatg gacattgatt gttggagccg tgatcggagc
    19741 aattgcaggt gcgatcacca gtaaaggaaa ttcgatgggc tgtgtaacga atatcattgc
    19801 tggactagtc ggttcaagta ttggacaagc gattttcggc gaatggggac caaaagctgc
    19861 aggtatggca cttattccat ctattttagg cgcagtgatt ttggtagcag ttgtctcatt
    19921 tttctttgga aaacgatcat aagtttttac aaaatagtaa actaaaagga gttgcagcga
    19981 gactgtgact cctttttggc tttatt
//
LOCUS       DACCQI010000057        19776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCQI010000057 DACCQI010000000
VERSION     DACCQI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.058"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..288
                     /locus_tag="IVO76_001588"
     CDS             <1..288
                     /locus_tag="IVO76_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7429555.1"
                     /translation="ETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRT
                     PYGMIQIATFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            364..984
                     /locus_tag="IVO76_001589"
     CDS             364..984
                     /locus_tag="IVO76_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7429556.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     EKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            1173..1679
                     /locus_tag="IVO76_001590"
     CDS             1173..1679
                     /locus_tag="IVO76_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7429557.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            1696..2595
                     /locus_tag="IVO76_001591"
     CDS             1696..2595
                     /locus_tag="IVO76_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7429558.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKARR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            2682..3567
                     /locus_tag="IVO76_001592"
                     /pseudo
     CDS             2682..3567
                     /locus_tag="IVO76_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(3759..3830)
                     /locus_tag="IVO76_001593"
     tRNA            complement(3759..3830)
                     /locus_tag="IVO76_001593"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3795..3797),aa:Arg,seq:ccg)
     gene            3890..5224
                     /gene="rpoN"
                     /locus_tag="IVO76_001594"
     CDS             3890..5224
                     /gene="rpoN"
                     /locus_tag="IVO76_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7429559.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            5427..6464
                     /locus_tag="IVO76_001595"
     CDS             5427..6464
                     /locus_tag="IVO76_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7429560.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            6505..7506
                     /gene="gap"
                     /locus_tag="IVO76_001596"
     CDS             6505..7506
                     /gene="gap"
                     /locus_tag="IVO76_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7429561.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            7616..8806
                     /locus_tag="IVO76_001597"
     CDS             7616..8806
                     /locus_tag="IVO76_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7429562.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            8910..9665
                     /locus_tag="IVO76_001598"
     CDS             8910..9665
                     /locus_tag="IVO76_001598"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7429563.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            9781..11079
                     /gene="eno"
                     /locus_tag="IVO76_001599"
     CDS             9781..11079
                     /gene="eno"
                     /locus_tag="IVO76_001599"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7429564.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            11478..12617
                     /locus_tag="IVO76_001600"
     CDS             11478..12617
                     /locus_tag="IVO76_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7429565.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(12684..13259)
                     /locus_tag="IVO76_001601"
     CDS             complement(12684..13259)
                     /locus_tag="IVO76_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7429566.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(13264..14238)
                     /locus_tag="IVO76_001602"
     CDS             complement(13264..14238)
                     /locus_tag="IVO76_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HAP7429567.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(14262..14306)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            14521..15489
                     /locus_tag="IVO76_001603"
     CDS             14521..15489
                     /locus_tag="IVO76_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7429568.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            15586..15879
                     /locus_tag="IVO76_001604"
     CDS             15586..15879
                     /locus_tag="IVO76_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7429569.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            15997..16233
                     /gene="secG"
                     /locus_tag="IVO76_001605"
     CDS             15997..16233
                     /gene="secG"
                     /locus_tag="IVO76_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7429570.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            16345..17091
                     /locus_tag="IVO76_001606"
     CDS             16345..17091
                     /locus_tag="IVO76_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7429571.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            17094..19451
                     /gene="rnr"
                     /locus_tag="IVO76_001607"
     CDS             17094..19451
                     /gene="rnr"
                     /locus_tag="IVO76_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7429572.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            19473..>19776
                     /gene="smpB"
                     /locus_tag="IVO76_001608"
     CDS             19473..>19776
                     /gene="smpB"
                     /locus_tag="IVO76_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7429573.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNT"
BASE COUNT         6813 a         3592 c         4200 g         5171 t
ORIGIN      
        1 gagacgggcg atgagattcc tgtaacgatc aaattagttc cagatggtca tgtacagctg
       61 atccgtgcag gagaaatgcg gatgcgtttg aaatttggct ataaagaacg gttagaaaca
      121 tcctatcgaa caccatacgg tatgatccag attgctactt ttacgaaaga actgcatgtt
      181 agtttgaaag accgtcccac agcagggaag gttcgaatcg attatgatct gttcatggga
      241 ccggaaaaaa tcggcgaata ttatttgacc ttggatttta cagcgtaaat ataaaagaaa
      301 agtttgattt tttgtgtctg attttgtatg ataaggagta gactgtgaaa ggacgtgtcc
      361 tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg aattatcaat gatcgaagta
      421 gcacacgcga tcttagaaca acgtggcgat gtcatggatt tttctgattt agctaaccag
      481 atccaaaact atcttgagaa atcagacagt gaaattcgtg attcactcgc acaattttat
      541 actgacttga atattgacgg cagctttatc tcattagggg ataatcgttg gggcttgcgt
      601 tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc atggaatgga cgaagaggat
      661 gaagatactc cacgtcgccg taaacgcaag aaagtcaacg cctttatcaa tgatgatgaa
      721 gacgcgatcg attacaatga cgatgaccca gaagatacag atctgacaga agaagatgac
      781 gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa tcgctgctta taattctgac
      841 ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag aagacttgcc aggaaatatc
      901 gaagaagatt taagtatcat agacgaagac gatgatgatg aagattatga agaggaagaa
      961 ttttctgact ctgaaaaaga ataaacatta ttttcttacg agaggttgtg acagagacga
     1021 tcagcctatc agaataagga ggtgtgacac gatttttgtc acaccttctt ttttgtatat
     1081 aaaaatccca agtaaataca taaaaaataa aaaaattgaa acttttagcg caccttgtac
     1141 gtcatattaa gtgaggcaga aaggagtgga gaatgatttt tactggatat aagaaaaaac
     1201 ggcagatcga aaaataccgt cacctgctga atggactcat cgaaactgat tatcaaaaaa
     1261 tgttccgtat cacgatgaat tacgtacatg ataaagaaga ggccttagat gtgatgcaag
     1321 atagttttca taaagccttg aactattttg aaaaagaaaa agagatcgag cattttagtg
     1381 catggtttta tcggatattg atccgcacgg cattagatag ttggcgcaga caaaaaagga
     1441 cgcctgtgga cttatttgaa attcaagata tcccccaaca gttaccttta gaaacttccg
     1501 ttgcagagct ccatatgatt ttagataaaa tagacagtcc ggaaaaagag attctgatcc
     1561 tctacttttt tgaaggattt cgtttgaaag aaattgcaac tatcttggat atgaatgaaa
     1621 acacagttaa aacgaaaatg taccgctcat tgaatgcttt acgtcaaatc ctagaatgaa
     1681 ggagagaaga gtgccatgta ttcccaaaaa gaaaatttaa aaaaactaaa gaaaacttat
     1741 aaagaacaag caattcccaa agggcttaaa gaggagatcc gccagcgttt tattatcgaa
     1801 gagcaatcat ttataaaagc aaggcggaga aagagacgtc tccaagggtt cggagtatct
     1861 ttggctatcg taatgattac aacaagtagc cttcttttta atagtcaagt tcgaagcttt
     1921 gcagaagatt tacccatctt aggatcagtc attgaattga tcttaggcga acgctttact
     1981 gatcgatctg aaaaaatcga tattcaggta ccgaagatca atacacaaaa tgagaaagaa
     2041 aataaaacaa ttcatggatt aaatcaaaaa tactttcgcg aaggacaagc agattttgaa
     2101 aaagctgaaa aagaatatgg aaattttgaa acggatcatt accaagtttt gggagattat
     2161 cagaaaatcg tagacgataa ccggtttctc gtgattgaaa ggcagatcac ccaaacagca
     2221 gcagatagcc atgttgaaaa aagatacgac acaatcgaca agaaaaacag tgtccagctc
     2281 tcacttccac ttttattcaa agatgacact tatctatctg ttttgacaaa agaagtcaaa
     2341 cggcagatgg ccgaacaagt caaggaagat ccatctaagt actattggac tgagcaagat
     2401 attcaagaag ggacgataga aaaaccaaca cttgttacgc ctacacgaag tttttatttg
     2461 aacaaagagc atcaattagt tttgactttt tctcaatacg aaattgctcc tggttatatg
     2521 ggtacaccag aatttgtcat tcctaagtcc gtgacaaaaa cgattttggc atcggaagat
     2581 tatttggatc attagacgag aagtagttgc gactagcagt ttcaattttc aagtaagtac
     2641 aagtaaataa taagaagaga aaaagagaaa gaagagatat agtgaaaaga aattatttag
     2701 tcatcagtat tattgcttta tccgtgcttt tgtttttcga aattacttcg gtggcctcct
     2761 tgatcaccga agtaaaagga caaacacact cttcggagca ttcgtcacaa tctttcaaac
     2821 aatcagaaca atcaaccatt aaaagccaat caactgaaac agctaacgtc aaagaagcca
     2881 ttcaagaatc agcggaaaag gtacaaagtc aatcatcaca atcaacacaa gacagtacag
     2941 aagcaatcca agacccgact gtcaaaagcg tcgcaatcag ctttgatgat ggaccaggag
     3001 cgaccacaac gccgcagctt ctgcgaatct taaaagaaaa aaacgtgcat gtcacttttt
     3061 tcgtcttagg agaaaatacg gcgcagcatc ctgaaatcgt gaagcagaca gctgaagcag
     3121 ggcacgagat tggcaatcac acatacgatc atcagaatct ggccactctt tctgcccagt
     3181 cgatgacgga ggaagtgacg aaagcagaca ctgagatcaa aaaaagtcac tggcaaaacc
     3241 cccgcttttg ttcgtccgcc ctatggttct gtcacgaatg taggagcaac gatcatccag
     3301 cgaccgatca tcgaatggtc tgttgattca gaagattgga agacaaggaa tcctgatttg
     3361 attttgcaga agatccaagc aacagtatac gatgggtcaa ttattttatt ccatgatatc
     3421 tatcctgaaa cgattcgtgc cgttcctcaa gtgatcgatt atctgaaaga gcaaggctat
     3481 cgcatcacga cagttagcga tttattaggg catccaacgg ctgtcgagaa ctattatggc
     3541 agaaacgatc atcggccagt tcagtaagtg agacaagtat ctttaataaa taacacattt
     3601 taataagtag ttgaacacat aactacctta cttgcaaagg cttaacaata tctatacaga
     3661 tacaacaaaa aactgtccac caattctctt tggcggacag ttttcactta aaaataaaga
     3721 agcaactatt cgtaaattat gctaaaatag cagtagaacg cgtcctgagg gaatcgaacc
     3781 cccgtctcaa gaaccggaat cttacgtgat atccactaca ctaaggacgc aagcactggg
     3841 ataattgtaa ctaaacagat aaaaaattac aaggggattg gacaaaaaaa tgaaatttga
     3901 gcaaaatttt tcacaaaaac agcagcagac acagaaacta gccatgacgc aacagttgca
     3961 acagtccatc caagttttac aatataacag tgaggagctg ttggcattcg ttgagaatca
     4021 agcaatggaa aatccattgg tggaagttgt tgagccggaa tggcagccag attacataaa
     4081 agcttcttca tcttcttatg aaggagaaga gacaaattac ctaaatcaga ttcctgatac
     4141 aaaagggtct ttatttgatt cattgatcga acaagtccat ttgaattacc gagatacttt
     4201 tttgaggaaa atcgttcttt atcttgtaga atacatcgat ttgaacggat ttttgacgat
     4261 tagtttagaa gaagccggaa aagagattgg agctacgcct atccaaatgc tcgatgcgtt
     4321 gactctgatt cagcaattag aaccagctgg agtaggtgca cgtagtttgc aagaatgtct
     4381 catgcttcaa acagaacgag atgattatgc gccggaatta gcttatatcg tgttggaaga
     4441 atgttttgag gaactggtgg agcgaaaatg gaaagaaatc gcacaacgtt tttctgttga
     4501 tctacatgct gttcagcaga tattcgacta tttacaaacg ttgagtccaa gccctggccg
     4561 gattttcgac cgtagcagcg aattgtacat tattcctgat gtccgggtat tagtggacga
     4621 cgataaaaat gtacaagtta tctcaaaccg aaaaaatcag ccaaatatac gctttcaaga
     4681 gagttacttc aaacaaatgt cacaacaagc agataaagaa actgaaaatt atttaaaaga
     4741 acggaaacag gaattcgaat ggttgaagaa aacgatcctc caacgtgggg atacaatttt
     4801 gcgggttgct caagtcattg tttcccggca aaaagctttc tttatcgaca aggaacgtcc
     4861 aatcaagcct cttacgttaa aggaagttgc aactgagata gatgtgcatg aatccacggt
     4921 cagtcgtgca gtcaacggga aatatttgga aacagatttc ggtgtatttg aattaaaaaa
     4981 atttttcact acacgaattc ctacgaatag tacagaacag acagaagacc tttcagcaga
     5041 tacagcgaag aaaaagctgc aggaattagt cgatcaagaa gacaaaaaca agccgttgtc
     5101 ggaccagaaa ttggttgaat tgctgaaaaa agatgagata gcgatctcaa gacgaacagt
     5161 agcaaaatat cgtgatttat taggaatccc gagttcatca aaaagaaaac gttatgataa
     5221 ctaatcagtc aaaagactag aaaaagtatt tcactttttc tagtcttttt agttgaaaaa
     5281 cgagagcaaa actgttagac ttacagtcgt gaggttgaga tttcccttta ttgatgtgaa
     5341 attcgaatag ttaatttctt tttttatcgt atatgggtcg ttttaagtct acattggacg
     5401 taaagtgtcc aactaaggag atcgctatgc tggatgaaat aaaaatgatt gaatctgttg
     5461 caccagatat tatcgaagtg ttgcaagaac gctataaaat tttacgaaat atctattgga
     5521 tgcagccgat tgggcgtcga agtttatcgg aaagtatggg gatcacagaa cgagtcttac
     5581 gaacagaaac agaccttttg aaaaatctga atttgatcga cacctcaaaa agcggtatga
     5641 cgctgacaac taagggcgaa gaagtttatc aaagccttga aaacttcatg gatcagcttt
     5701 taggtacgca tcaaaccgaa caacagttgg cagaatattt tggtaaccaa cgctgtatcg
     5761 ttgtttcagg aaacagcgaa gagcagacca aagtggctga tgcattcggc gaagcgctga
     5821 gcgaagcctt agaccgacta cttccagaag gcgagaatat catcgctgtg atgggaggaa
     5881 ctactatggc tgtggtagcc gaacaattgt ctaatctgga aaataagaaa cggcataacc
     5941 tgtttgtccc tgcaagaggc gggattggtg aagcgataac tgtacaggca aattctgtca
     6001 gtgcaagaat ggctgctaaa gcaaacggta accaccgcgc actttatgtt ccggaacaat
     6061 taagtctggc aacttacaat tctttactca atgagccatc tatccaagaa gtcttaaact
     6121 tgattagtga agcgaattgt gttattcata gtattgggcg tgcattacac atggcagcac
     6181 gtcgtaagat gactgaaaaa gaattagtca tgctgaaaca agcaaatgct gttgcggaat
     6241 cttttggcta ctttttcaat gaaaaaggtg aagtcgttta taaggtccct agaatcggca
     6301 tagaattgca agatttggaa aaagtcccga tcatcatggc aatcgcgggc ggaaaatcaa
     6361 aagctaaagc aattcgagct tatatgaaaa atgcaccaaa acaaacgtgg ctcatcactg
     6421 atgaagctgc tgcaaatgag attttaaaag gggtaaccct ttaaaataaa tatttttttg
     6481 attttcataa ggaggaaatc ttaaatgaca gttaaagtag gtattaatgg ttttggacgt
     6541 attggacgtc tagcattccg tcgtatccaa gatgtagatg gaatcgaagt agtagcaatc
     6601 aacgacttga cagatgctaa aatgttggca cacttgttaa aatacgacac aactcaagga
     6661 cgtttcaacg gaacagttga agttcatgaa ggttcattca acgtaaacgg aaaagaagtt
     6721 aaagtattag ctaaccgcaa cccagaagaa ttaccatggg gcgaactagg cgtagatatc
     6781 gttctagaat gtactggttt cttcacttct aaatcagcag ctgaaaaaca tttaacagct
     6841 ggtgctaaac gcgttgttat ctctgctcct ggcggaaacg atgttccaac aatcgtttat
     6901 aacacaaacc acgaaacatt aactggtaaa gaaacagtta tctcaggtgc ttcatgtact
     6961 actaactgtt tagctcctat ggctaaaaca ttgaacgaca aatttggtgt tgttgaagga
     7021 ttaatgacaa ctatccacgc ttacacaggt gaccaaatga ctctagacgg acctcatcct
     7081 aaaggtgact tccgccgtgc acgcgctgct gctgcaaaca tcgttcctaa ctcaacaggt
     7141 gctgctaaag ctatcggttt agtaattccc gaattgaacg gtaaattaga cggagctgct
     7201 caacgtgttc ctgtaccaac aggttcatta acagaattag taacagttct tgaaaaagaa
     7261 gtaactgttg acgaaatcaa tgcagcaatg aaagaagctt caaacgaatc ttacggatac
     7321 aacacagacg aaatcgtttc ctcagatatc gttggtatga cttacggttc attatttgat
     7381 gctacacaaa ctaaagtaat gacagttggc gacaaacaat tagttaaaac tgttgcttgg
     7441 tacgacaacg aaatgtcata cactgcacaa ttagtacgta ctttagaata cttcgctaac
     7501 ttataagata gacaaacgta caagtgtaaa ttatagacat gtacatttaa aagcggggaa
     7561 gcaatcgcgc ttcttccgct ttttttgata ataagtttat tcaggaggaa ataaaatggc
     7621 taagaaaaca gtgaaagata tcgatttaaa agacaaaaaa gttcttgtcc gtgttgactt
     7681 caacgttcct ttgaaagacg gcgtgatcac tgacgacact cgtatcaaag cagcattgcc
     7741 aacaatcaac tacgttctag aacaaggcgg aaaagcaatc cttttctctc accttggacg
     7801 tgtaaaaaca gaagaagata aagaaggaaa atctttagct ccagttgcaa aacgtctagg
     7861 cgaattatta ggtaaagaag taacattcgt acctgaaact cgcggagaac aattagaaga
     7921 agcaatccgc aacatgaatg acggcgacgt tgtcgtattc gaaaatacac gttttgaaga
     7981 tattgacggt aaaaaagaaa gcaaaaatga tacagaacta ggtaaatact gggcttcatt
     8041 aggcgatgta tttgttaacg atgctttcgg tacagctcac cgtgcacatg cttctaacgt
     8101 aggtatcgct tcaactggta tcccaacagt tgctggattc ttgatggaaa aagaaatcaa
     8161 gttcatcggt gaagcagtag aagaaccaaa acgcccaatg attgcaatcc ttggtggcgc
     8221 aaaagtttct gataaaatcg gcgtaatcga aaacttgatt cctaaagcag ataaaatctt
     8281 aatcggtggc ggaatgactt acacattcta cgaagcaaaa ggaatcaaga tcggtaactc
     8341 tctagtagaa gctgataaag tagaactagc aaaagaatta atcgaaaaag caggcgacaa
     8401 acttgtcttg ccaatcgaca atgtctgtgc accagaattt tcaaatgatg ttgaaacaca
     8461 agtaatcgaa ggagacattc ctgatggttt gatggcttta gacatcggcc cagcatcagt
     8521 aaaattattt gctgatacat tacaaggtgc aaaaacagtt gtttggaacg gacctatggg
     8581 tgtatttgaa atgagtaact ttgctaaagg tacaatcggt gtttgtgaag caatcgctaa
     8641 cttagaaggc gcaacaacaa tcatcggtgg cggagactca gctgcagcag cagaacaatt
     8701 aggattcgct gacaaattca cacacatctc aacaggtggt ggcgcaagct tggaattgct
     8761 tgaaggtaaa gaattaccag gactagcagc aatcaacgat aaataaacga ccaagtttgc
     8821 tcaattctga cgagtaatcg atcaaaaggt gaagggagtt ttcatgactc cctagccatt
     8881 tttcaataaa ttaaggagtg tgttcctcaa tgcgtaaacc tattatcgca ggtaactgga
     8941 aaatgaacaa aactgcatca gaagcaaaag aatttgctga agcagtaaaa aacaaaatcc
     9001 cttcaaatga caaagttgat tcagtcatcg gatctcctgc attattctta caagaattaa
     9061 tgacatcagc tgaaggtaca gaactaaaaa tctcagcaca aaactgctac tgggaaaact
     9121 caggtgcgtt cactggtgaa acttcaccag cagctcttgc tgatctaggt gttcattatg
     9181 tgatcatcgg acattcagaa cgtcgtgaat atttccatga aacagacgaa gacatcaata
     9241 aaaaagcaaa agctatcttt gcaaatggta tgacaccaat cctatgctgc ggcgaatctt
     9301 tagaaaccta tgaagcaggc aaaactgctg aatggatcga aggacaaatt acagctggtt
     9361 tagctgatct ttcagctgaa caagtgtcaa acatggttat tgcttatgaa ccaatctggg
     9421 caatcggtac tggtaaatca gctgacgcac aaatcgctga cgaaatttgt ggagttgtac
     9481 gtcaaacagt tgaaaaactt tatggaaaag aagtatcaga agcagttcgt atccaatacg
     9541 gcggttctgt aaaaccagaa aatattgctg aatacatggc aaaagaaaat gttgacggtg
     9601 ctttagtcgg tggcgcaagc ttgcaagcag attcattctt gtcattgtta gacgctgtaa
     9661 aataattgtt gaaaatttgt gaaaaatgca agaaatgttt gcactaatga gcaaaattaa
     9721 ctaaaatcgt atatgaggga aatcccttag gaaaaaacaa actcaaagga gagacaaaac
     9781 atgtcaatta ttactgatgt ttacgctcgc gagatcttag actcacgtgg taacccaaca
     9841 atcgaagtag aagtatacac agaaagcgga gcttttggcc gcggaatggt tccttcaggt
     9901 gcttcaactg gtgaatacga agcagttgaa ttacgtgacg gagacaaagc tcgttatggc
     9961 ggtaaaggtg taactaaagc tgtcgacaac gtaaacaaca tcattgctga agcaatcatt
    10021 ggctacgatg ttcgtgacca aatggctatc gacaaagcta tgatcgcttt agacggaact
    10081 cctaacaaag gtaaattagg tgcgaacgct attttaggtg tttctatcgc tgttgcacgt
    10141 gcagctgctg actacctaga agtaccttta taccactacc taggcggatt caacacaaaa
    10201 gtattgccaa caccaatgat gaacatcatc aacggcggat cacatgctga taactcaatc
    10261 gacttccaag aattcatgat catgcctgtt ggcgctccaa cattcaaaga agctctacgt
    10321 atgggtgctg aagtattcca cgcattagct tcaatcttga aagctcgcgg attagcaact
    10381 tctgttggtg acgaaggtgg atttgctcca aaccttggtt caaacgaaga aggttttgaa
    10441 gtaatcatcg aagcaatcga aaaagctggc tatgtacctg gtaaagacgt tgttcttgct
    10501 atggatgctg cttcttcaga attctacgac aaagaaaaag gtgtttacgt actagctgac
    10561 tcaggcgaag gcgaaaaaac aactgacgaa atgatcaaat tctacgaaga attagtttct
    10621 aaatatccaa tcatctcaat cgaagatgga ttagacgaaa acgactggga cggattcaag
    10681 aaattaactg acgtattagg cgacaaagtt caattagttg gtgacgactt gttcgtaaca
    10741 aacactcaaa aattatctga aggtatcgaa aaaggaattg ctaactcaat ccttatcaaa
    10801 gtgaaccaaa tcggtacatt aacagaaact ttcgaagcta tcgaaatggc taaagaagct
    10861 ggctacacag cagttgtatc tcaccgttct ggtgaaacag aagattcaac aatctctgat
    10921 atcgctgttg caacaaacgc tggccaaatc aaaactggtt ctctatcacg tactgaccgt
    10981 attgctaaat acaaccaatt actacgtatc gaagaccaac ttggtgaagt tgcagaatac
    11041 aaaggtttga aatctttcta caacttaaaa aacaaataat tcttttctaa tgaagataca
    11101 tgatcgacta ttgaaagata gtcgagtatg atcacttagt taagtaatcg aactctctac
    11161 ttgctgaaat atcagcaggt agagagtttt tttgcaacta gcttctgtat atgatgttta
    11221 caaagaatgg tacaagaaaa gatcatcctt gaaaaaggaa agctaacaat aactagcatc
    11281 atataagagc ttttaaggat agaacagtag aataggaatc tatgagcaag tcctctcgta
    11341 taaataaaat aggagggaag tagcagcata tttttcataa agtggctctc tttcttcatt
    11401 tttactttgg ttgctcctat ttgaaaattc gagcacactc aaatacaata aaaagaaatg
    11461 gcaagggaga ggattagatg aaaaaagaag aaaaaatcgc gattttacaa gagatcatcc
    11521 gaattaaatc agtgaatgga aacgaaggtg aagtagcagc ctatttgaat aagcttttgg
    11581 caaaacatga cataacagga gagattgttt cttacagaga tggtagagat aatttgattg
    11641 ctcgttacca aaagggccaa tcaggaaaag tgttaggcct atctgggcat atggatgtcg
    11701 tggcggctgg tgatgaatcg tcttggacct atgcaccctt tgcagcagaa atccatggga
    11761 accgtttgta cggacgtggt gctacagata tgaagagtgg cttggcagca atggtgattg
    11821 ccatgatcga actgaaagag tcaggaaaac catttaacgg aacagtcaaa ttattagcca
    11881 ctgtcggaga agaagtgggt gaactaggtg gggaacagct cacgaaagca ggctatgtag
    11941 atgacttaga tgccttgatc atcggagagc caaccaatta cagtttgatg tatacgcaca
    12001 tgggatcaat caactataca gtcacctcgc acggaaaaga agcgcatagc tctatgccag
    12061 accaaggtta taatgcaatc aatcacctaa atgaattcat tacgaaagca aatgctgaaa
    12121 tgaatcacct cgctgaaaca atcgagaatc ctgtcctagg aaaaacaatc cataatgtta
    12181 cattgatcag tggcgggaat caagtcaaca gcatcccaag ccatgcgcag ctccaaggga
    12241 acattcgatc gattccagaa tacccaaatg ataaaatcat tgcattgctg caatcaatcg
    12301 tcaatgaatt gaatcaagag acagactatc atttggaatt gatgatcgat tacaacaaaa
    12361 tacctgtcaa agctgatcca gattctcctt tgattcattg cattcagcaa caattcagcc
    12421 agccattacc tttagttgga gcggcagcaa caacggatgc agccgaattt acgaaagcta
    12481 accattcctt cgactttgtc gtctttgggc ctggtgtcgt tactttgcct catcaagtag
    12541 atgagtatgt tgaaatcgat aattatttgg atatgatcga aaaatatcaa ggaattattt
    12601 tatcctactt agcgtaagat ttttttaata aatgaaaaaa cagagccaga ttagcattat
    12661 tttgctaatc cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgt
    12721 ggcaacatag tgatgactaa tgaaacaacg acgatagaca acaaacgatc aaggtagtct
    12781 gtacctgcct gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc
    12841 tgaacgatca tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc
    12901 gaagaaacga tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg
    12961 tttttatctt tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt
    13021 tggattggcg aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg
    13081 gacaatcctg tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg
    13141 atagaatcga gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg
    13201 atcccaatac acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt
    13261 ttgttagccc atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa
    13321 aagaacggtt aaaaactttc ggaaaaaaag acgagtatct acttttgtca taattttact
    13381 gttagcaggt ttctgccaaa attcataacg atcaaccaat gtttgtccac gacttattcc
    13441 ttgagtttcc acagcagtga agcttttgaa accttcacag atggcctcct cgataaaata
    13501 agcaacagct aacggatcat tgatgacaca tcctaaaatt ctttcttgtt tccagtggaa
    13561 gtcaaaataa aaacgagtga tcgcttttag atactctccc tcttcaggat tcatttgaca
    13621 gcagtattcg aggatagtcg gtgtcaagac gatttctctt gtcacatcta acccaaccat
    13681 ttctatcgtt tgtttgagat tctcgaatac ataagaagct gcttcaggat cacaccagta
    13741 attgtattca gcaactggcg aacagttacc atggcttttg taagttccac ccatcgagac
    13801 aaaacgttcc atatgtttcc caatatttgg gttgactttc aaagcagaag cgatattggt
    13861 caaaggaccc aaagcaatga ctgatgtatt tgtcttttct ttgaaataat cagccaaaaa
    13921 atcaacggca tggagaggtt ctgcctgttt tctcaagatc atcgggaaat ttgtttcccc
    13981 taaaccatcc ataccatgag tgtcttgtgc actgatgaaa ggttgcttca aaggtttttc
    14041 cattccttga taaacaggaa tgtctaaacg atcacagcgt tccaggcatt tcagcacatt
    14101 ctgcgtacct atatctgcag ggacattccc acaaactacg gtaatagcaa ctacttcgat
    14161 atcaggggat ttgacagcta atgataaagc taaagtgtcg tcgatccctg gatcgcaatc
    14221 aataattact tttcgcatta tttccaatct cctttcttta gagatactta gttttttatt
    14281 ctgggaagtt tccgaaccag tccaagacga gcgtcttgat tataatgcac gaaattagta
    14341 tactggcttt tattgcgaat gtaaatgctc tcttacaagt aaatctattg aggagaactt
    14401 taagtaaatg atacaatgaa aaagaaatgg aatttacgtg tgccgaaaat aaagaatcct
    14461 atggatcggt acattggata atagatagat tatagtttgg cagaaagcgg cgggtgtaca
    14521 atggttattt tttatttcat tcttattctg ataattatag ggatcagtta ccattttctt
    14581 tcttattttt atgcaaagaa attagtaaga gcgggagtac agagaggata tgactggtat
    14641 gaagacactg gtcatcgttt gatggaccct gctgcagtca cgtcagtaga aaaaaaacga
    14701 caaaaattag aagaagagtg tgaaagcttt tgggagcagg gaacacccaa aacaatcaaa
    14761 agttatgacg ggttgaaact agctggtcag atgttcatcc atccaaataa gcagaataaa
    14821 tgggtcattt gcgtacatga ttaccgaagc acagggaaaa gagacatgtc tcatataggt
    14881 aaaagatatg cagagaaagg attcaacgtt cttgtacctg atttacgtgc gcacggagaa
    14941 agtgaaggag aaatcatcgg aatgggatgg ctagatcggc tagatttaat tgcctggatc
    15001 caactcatct tagatgaaca gccgaatgcg tccattattt tacacggcgg atcaatggga
    15061 gcttctacga tcatgatggc aagtggtgaa aaacttccat cagcggtcaa aggatttatt
    15121 ttagacagcg gatatgtttc tgtttatgca gaattccgat atatgttaag taaaatcact
    15181 gtctttccta aaaaaatggt catgagatat gcgaatcatt atgcccagaa atacgccggt
    15241 tactccttaa agcaagcatc agctacgaga cagcttggca gcaatcatct gcctttatta
    15301 gtcatccatg gtgaaagaga tcattttgtc cctacagaag cagcgtacac gattcaaaat
    15361 gctacggcag gggataaagc actgctgctt gttccggaag cagaacactt ggaagcatcg
    15421 atgaaagatc cgaaaaccta ttggacagtg attttttcat ttattgaaca aagaataacg
    15481 atgaattaat tttctcttct tttttgtaag taattcgagg ataaataatt gtttatagat
    15541 caggagactg ctaaaataag gtaggaataa aaaaatatca gttatttgag gagtgcggca
    15601 tatggaaatc aaagaagaaa aaatcgtctt gctttattca atgatgaaca acaagagatc
    15661 ggagaaatga catggtcaga tgcgggccca gacatcatga tcattgacca tacatttgta
    15721 gatccagcat atcgtggtca aaagttagct gaaaagctag tgtatactgg agtagaatta
    15781 gcaagacgag acgggaaaaa gatcattcct ttatgccctt atgcaaaaaa agagtttgaa
    15841 aagaaaccag aatatcaaga cgtcttacgt cagtcataag aataacagct gaaagttttg
    15901 aactttcagc tgttattcca tttatataga gttgtcacgg acttttatct atgataaagt
    15961 taaagagacg atatcttttg ggaaggaggc tgcgatatgt ataatttaat tttgggaatc
    16021 gtgatcgttt tatcgattgt tatggtcatt gcgattatga tgcagccaag caagcaaaat
    16081 agtgcagcga gtgcatttac cggcggtgcg gatcaactat tcggcaaaca aaaagctcgc
    16141 ggctttgaag ctgtcatgca gcgatcaact gcagttatgg gagctatctg gatgatctta
    16201 ttatttgtcc tagcatattt atcttcgaag tagttgaata agcctctcgc aaaacggcgg
    16261 gcggcttttt ttattgcaaa gagaacgctt aagaagaagt tatgaaagaa actatggtaa
    16321 aatgaggcga aagaggtgag acgagtgaga caattaccaa aaccccttta tgcaaaacat
    16381 ggaaagcgag cggttttgct gcttcacgct tactcaggaa gtccaaacga tgttcgaatg
    16441 ctcgcacgat ttttggaaaa agcagattat acagtttatg cgccgctttt taaaggacac
    16501 ggaacaatgg agccatatga tatcttgcaa gaaaaagcag aaagctggtg ggcagacact
    16561 aaaaaagcca tccatttttt acagtctgag cagttttctg atattgccgt attaggatta
    16621 tcaatgggcg gcatctttgc tgtacgagca ctagaagaag aatcagtgat tggcggcggt
    16681 tttttctgtt cgcctttgtc tcctgtaaaa acaaacgtgc ctgaaaactt tgaaaaatac
    16741 gtacgtcaag tgctgaagac agcaggtaaa tcagagaaag aaatcaatga aaaagcagtt
    16801 gcttaccgct cgttagctga acaacaatta atggatatac aagatcaggc agcaatcgta
    16861 gaaagtaggt tgtcagatat ccaacagccg atatttttag ctcaagctgg gaaagatgaa
    16921 atgatcgacc ctaatggcgt atttgaaact gcacgcaagt tgagccgtca gcgagtgaca
    16981 cttcaatggt atcctgaaag tggtcatgtg attacagttg gtacagcaag acgcgaatta
    17041 gaaaaagacg tcttggaatt tttagaaaaa ttaccttgga atgaggaata aatatgacaa
    17101 aagaaacgat aaaagagaaa attctctttt tcatggaaag cagtcgcaaa cgcagttttt
    17161 ctatggaaga aatcgctgaa ggtttagggt tccaaaaaag cgatgatttt aaattactag
    17221 tacaaacgat tgctcaaatg gaaagagaac agagtatagt tttcaataaa aaaggaaaag
    17281 tgaaacttcc tttaaaacca atcttgatcg aaggaacatt tcgagcaaat gaacgaggat
    17341 ttggatttgt aacgattgat cccgaagaag atgacatcta tatcccaaaa gaagcgactg
    17401 gttttgcaat ggatggagac accgtagcaa tcgatatcat gaagcgagca aatacagata
    17461 tggatcgcgg agcagagggg aaagtagtcg agattcgaga ccgggcgact actcagttag
    17521 caggggaatt cgttgcttat tcagaagaag aaatacaaga aacagatctt tacggcgttg
    17581 ttatcccgaa agatagaaag ctcaaccaat ttaaagtata tgcagcagca gagggagttc
    17641 gtccagttga cggaagtatt gtgctgatcg aactgaccca ttaccctgaa aaagggtatg
    17701 cgactagttt agaagggata atcaagcaag tgattggtca taagaatgac cctggcatgg
    17761 atatcctctc aattgtcgtg gctaatggta tccctacgaa atttcctgat gatgtattgt
    17821 ctgaagcaga tgaagttcca gatcaaatca gcgaaaagga tataactgga cgtagagatt
    17881 taagaaatca gctcattgtt acgattgatg gagaagatgc aaaagatcta gatgatgcag
    17941 ttacggttaa aaaattagat aacggtaatt attttcttgg agtacacatt gcagatgttt
    18001 cttactatgt gacacaaggc agtcaattgg acagagaagc atatgaaaga gggacaagtg
    18061 tatatttgac tgatcgagta attccgatga tcccgcaacg tttatcgaat ggtatctgtt
    18121 cactgaatcc ccatgttcca aggctgacca tgagttgcga aatggagatt gatccaaatg
    18181 gacaagtagt ctctcatgat attttcccaa gtgtgatcca aacaacagaa cggatgacct
    18241 acacagctgt caatcaaatc ttagaagatc aagacgaaga aacgatgcaa cgctatgctc
    18301 agctcgtacc tatgttccaa gaaatgggtg aattgcaccg aatccttgaa acgatgcgaa
    18361 tcaaacgtgg cgcgatttct tttgaggatc gcgaagcgaa aatccttgtt gatgctgaag
    18421 gacacccaaa tgacatccag ctaagaagca gaggaattgg tgaacgcttg atcgaatctt
    18481 tcatgcttgc ggccaatgaa acggtagctg aacattataa taaattaaat ctgccgttta
    18541 tttaccgaat acatgaacag ccaaaagaag aaaaaatgca gcgcttcttt gattttgctt
    18601 cagctttggg cattctggtc cgaggaacga aaggaacgat cacaccaaaa gatttacagc
    18661 atgtgatcga aggagtagaa gacaaaccag aagctgcagt tatcaatact atgttgctaa
    18721 gaagcatgca acaagcacgc tattcagaag acaattttgg gcattatggc ttagcggcag
    18781 attactacac acactttaca tctcctattc gacgctatcc cgatctaatc gtccatcgtc
    18841 tgatccgttc ttacagtgaa gataaaagtg aaagcaatca aagttcgtgg gctgcggaac
    18901 ttcctgaaat tgcagatcat agttctaaga tggaacgtcg tgcagtagaa acagaacgtg
    18961 aagtagattc catgaagaaa gcagaattca tgatggacaa agtgggagaa gaattcgatg
    19021 ggatcatcag ctctgttgtg aaattcggcc tgtttatcga actgccgaat acagtagagg
    19081 gattgatcca tatcaatgaa ttgaaacagg actacttcca ttttgttgaa aatcatttag
    19141 ctcttgtagg tgaaagaact ggtctgactt ttaaaatcgg acagaaagtg cgagtaaaag
    19201 tagtcaaagc tgatccagat gaacgagcag tagattttga acttgtttcc gcagaagagg
    19261 ttacagcgtt ggaacggcca aaaacacgaa atggaaaatc aggaaaacgc gaaggaaaac
    19321 aagcaggaaa acgcccagaa agaaatggca agaagaacaa acgatatagc gatagtaagc
    19381 aaacgttcgg taagaaaaaa gggaagaagc cattttacaa aggtgttaaa aaaaagaaaa
    19441 agaaaaaata aaatgagttg gaggaaacag ctatgccaaa aggcgaggga aaattaattg
    19501 cacaaaacaa gaaagctcgc catgattatt cgatcatcga cacgatggaa gcagggatgg
    19561 tcttgcaagg aaccgagatc aagtcgatac gaaacagccg gatcaatcta aaagatggat
    19621 ttattcgcgt ccgcaacgga gaagctttct tgcataatgt tcatatcagt ccttatgaac
    19681 aaggaaatat ttttaatcat gatccgttgc gcacgagaaa gttattatta cacaaaaaac
    19741 aaatcatccg gcttgaaaat gaattgaaaa atactg
//
LOCUS       DACCQI010000058        19743 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCQI010000058 DACCQI010000000
VERSION     DACCQI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.059"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            156..2627
                     /locus_tag="IVO76_001609"
     CDS             156..2627
                     /locus_tag="IVO76_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7429574.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2730..4466)
                     /locus_tag="IVO76_001610"
     CDS             complement(2730..4466)
                     /locus_tag="IVO76_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7429575.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4463..5167)
                     /locus_tag="IVO76_001611"
     CDS             complement(4463..5167)
                     /locus_tag="IVO76_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7429576.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5298..5801
                     /locus_tag="IVO76_001612"
     CDS             5298..5801
                     /locus_tag="IVO76_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP7429577.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5761..6099
                     /locus_tag="IVO76_001613"
     CDS             5761..6099
                     /locus_tag="IVO76_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7429578.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6120..7538
                     /gene="ffh"
                     /locus_tag="IVO76_001614"
     CDS             6120..7538
                     /gene="ffh"
                     /locus_tag="IVO76_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7429579.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7650..8228
                     /locus_tag="IVO76_001615"
     CDS             7650..8228
                     /locus_tag="IVO76_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7429580.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8232..8753
                     /locus_tag="IVO76_001616"
     CDS             8232..8753
                     /locus_tag="IVO76_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7429581.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8832..9386)
                     /locus_tag="IVO76_001617"
     CDS             complement(8832..9386)
                     /locus_tag="IVO76_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7429582.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9639..9914
                     /gene="rpsP"
                     /locus_tag="IVO76_001618"
     CDS             9639..9914
                     /gene="rpsP"
                     /locus_tag="IVO76_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7429583.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9926..10177
                     /locus_tag="IVO76_001619"
     CDS             9926..10177
                     /locus_tag="IVO76_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7429584.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10302..11093
                     /locus_tag="IVO76_001620"
     CDS             10302..11093
                     /locus_tag="IVO76_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429585.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11263..11784
                     /gene="rimM"
                     /locus_tag="IVO76_001621"
     CDS             11263..11784
                     /gene="rimM"
                     /locus_tag="IVO76_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7429586.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11774..12535
                     /gene="trmD"
                     /locus_tag="IVO76_001622"
     CDS             11774..12535
                     /gene="trmD"
                     /locus_tag="IVO76_001622"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7429587.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12671..13018
                     /gene="rplS"
                     /locus_tag="IVO76_001623"
     CDS             12671..13018
                     /gene="rplS"
                     /locus_tag="IVO76_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7429588.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13276..13569
                     /locus_tag="IVO76_001624"
     CDS             13276..13569
                     /locus_tag="IVO76_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7429589.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            14412..16739
                     /locus_tag="IVO76_001625"
     CDS             14412..16739
                     /locus_tag="IVO76_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7429590.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            16865..17194
                     /locus_tag="IVO76_001626"
     CDS             16865..17194
                     /locus_tag="IVO76_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP7429591.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     AEIKTSD"
     gene            17234..18238
                     /locus_tag="IVO76_001627"
     CDS             17234..18238
                     /locus_tag="IVO76_001627"
                     /inference="COORDINATES: protein motif:HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7429592.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            18235..19664
                     /locus_tag="IVO76_001628"
                     /pseudo
     CDS             18235..19664
                     /locus_tag="IVO76_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
BASE COUNT         6735 a         3611 c         3976 g         5421 t
ORIGIN      
        1 caacacaaaa tattatagaa cccttttttt ttgctatatg acaggaatga aaggtgtttg
       61 caagcttaat gtgaggaaca ctttccatat ctgttctata ctggatagga gaagtttgga
      121 aatatccaga aaacgaaatc aatcgaggta gccacatgaa gaataaatgg ataaatcgaa
      181 gtaaaaaaaa gaaagtaaca caaagatcat cagataaagt catccgtctt tttccatcgt
      241 ataaagaagg attaacggca gatcaagtca aggaacgaat agaaaaaggt gcagcaaata
      301 attcagtcga tcccacattc aaaacaaatc agcaaatcgt attggaaaac atatttacgt
      361 atttcaattt gatctttctt atcttagcca ttttgctttg tttagtagaa tcctataaga
      421 acttgacttt tttaccagtt atcatagcca atacaggaat cgccattttt caggaaattc
      481 gatccaaaaa gatcttggac gaactaaatg ttttaaatac agcaaaagtc aatgtcatac
      541 gagacgggat agaacaaaca atagatatag aagaccttgt aatagatgac attgttattt
      601 tagagactgg atatcaaatt ccagcagatg cagaggtcgt ggaagggaaa ctgcaagtta
      661 atgaggcttt attgacaggc gaagcagatg aaataaccaa agaaataggt gatccattaa
      721 tgtccggcag tttcattgtt tccggaaaag cttacgcccg attagacaaa gttggagcag
      781 attcgtatat ttcccagctg acagtcaaag ccaagacaat gggagaagga gaacagtcgg
      841 agatgattgc ttctcttaat caattgatca aatggattgg gatcatcatt attcctatcg
      901 gtatcctgct tttttctcaa agttactttt ttaatggaaa cacgatcaaa gaaagtgtcg
      961 ttgcaatgga agcagcattg atcggtatga tacctgaagg actgtacttg ctgacgacga
     1021 ttgcattagc attaagtgcg actaaactag caaagcaaag agttctttta cacaatatga
     1081 aaagcattga gacattggca agggtaaatg tcctttgtgt agataaaaca ggaacgatca
     1141 cagaaaataa aatgtctgtt caaaaaatga tcgtctctaa aaaacaagaa ataatcagtc
     1201 agtcagatca attggaagca tgcattgctg actatgcaaa agcgatggca ggagacaatg
     1261 caacaatgga agctgttaaa gcatatttta caaaaaccac tgatcaatcc tatcaccatg
     1321 ttattccttt ttcttctgtc caaaaattca gcagtgtagc gttggcagac aaggtgtatg
     1381 ttttaggagc gcccgaaatg gttttgcgcg atcaattgcc ggaatatgcg gaagagtttg
     1441 tttctttcgc tgaaaaaggc tatcgcgtgt tggtctttgg tgtgtacgat gggattcttg
     1501 aagaaccagt attgacagaa gcagtcacac cattaggcta tattctgatt gctaatccta
     1561 tcagaaaaga agcaagagcc acatttgatt acttcaaaaa acagcaagta gccatcaaag
     1621 tgatttctgg agataatccg ctcaccgtat ccaatgtagc tgtccaagca gggatttcac
     1681 atgctgatca gtacgttgat gcatctaaat tatcagaaga agaatatgaa gcagctgtcg
     1741 aaaaatatac agtttttgga cgcgtcaagc cagaacaaaa gaaaatcttc gttcaattga
     1801 taaagcgtaa caatacagta gctatgacag gtgatggcgt gaatgatatt ctagccatga
     1861 aagaagcaga ctgcagtata gcgatggctt ccggaaatga agccgccatg caggcctcac
     1921 aagtggtctt acttgattct gacttttcac gcatgccaga agttgttgca gaaggacggc
     1981 gcgttgtgaa caatattgag cgatcagcca gcttgttttt agtgaagaat attttctcct
     2041 ttttgctctc actgttttca gtgatctttg ctctgaccta tcctttagaa ccatcacaaa
     2101 tcacattgat tagtttgttt actatcggac ttccatcttt cttgcttgct ctagaagaaa
     2161 acaaaaagcg aatcagaggg aaattcatca tgaatgtgat ggaaaaagct gtgccaggtg
     2221 gactaacaga tatgatcgta gtcggtgcgt tggtgatctg cggggtgact ttgaatctaa
     2281 ataaaacaga tgtttctaca gcatctacca tgcttttgat tgcagtaggg ttcctcgttc
     2341 tctataaaat ctgttcaccg ctgaataaat tcagaagtca gatcattctt ttctgtgcta
     2401 gcggtaactt tttttcggtc atctttttgc ataagctgtt ttcaatcacc ggtatctcag
     2461 ctgtatcact tttgctgttg agcattctat ttttctcggc agattcgatt tttaggcatt
     2521 tgaccacatt ggtagaatat ttgtttgaac gtaaaaatga aaaagagcaa aagccgttta
     2581 gtcgcgtagc taaaaaattt ctatcgatat ttaaacgaga aaattgacga aaagtatctc
     2641 aaaaaagagt aaaaaagaac aaccactcgt gtatctttcg aagcaggatg agtcatccaa
     2701 ctttaaaacg cacgagcagt tgctcttttt tacattttcg gaatggtaac ggtgaaagtc
     2761 gaacctgttc caagatgact gtctacagta atcgtaccat taaggagttg cacgtagtct
     2821 tttacaatcg ctaaccctaa tccggtcccg cctgaatgtc ggcttcgcgc cttgtctacg
     2881 cggtaaaaac gctcaaagat ccgttcctga tcctcaatgt cgatacctat accaaagtct
     2941 tggacagaaa acgatagatc atctgttgcc tgatagcgga taataatctc gctgtcgctt
     3001 tttgaatatt ggacagcatt ttcaatcaga tttttgataa ttggataaag gatatctgtt
     3061 ttagtagtaa atggttcgtt ctttccaatc aatcgaatcg tcaaccgttt cttttcgatg
     3121 attggctgat aattttgaac gatttgctgg aaataaggtt ctgtataaat tgtctggatc
     3181 tcgtaagaat tctcgccatc ttttgacagc tggatgattt cccgaatcag tttatctaac
     3241 cggatcgcat ctttttgcat gatctcaaga aaagaagtca gagtttgcgg gtcatctttt
     3301 gcaccatcca gtaaagtctc ggtaaaacca atcagtgaag tcacaggagt ttttaattcg
     3361 tgagagacgt tgccaacgaa atccttttgt agcttttcta aacgtcgcac tttcgttaag
     3421 tcatacgcca cccctagaat ctgtccggta ccttctgaat tagtaaagta gcgcaaactc
     3481 aagtctaatg tctgccgacc ttctgtaagg gtgatctcct gatggatcag cggatgctca
     3541 ggagtcactt gatgaatcag ctgaatgagt tttggctctt gaatgacttc tgtgtaccgc
     3601 tgttcaggtt gatagcttac cacgtttaaa tgtagctgca tctttggatt aagcagtagt
     3661 aaacgagaat cagcatcatc aatcaggaat acgccaatca tcagttcgtt cagtaacgta
     3721 tataattgct cttctgtggt agtacacgcc cgatacatct tactcatttg ttcgctcaac
     3781 gcattaatcg tctgatacaa ttcttcccat tgatcgggtg tttgcatgat tacttccgcc
     3841 tgattaggat tttgcaccat tttttttagt acgggcagca cggttttcaa tggctggttt
     3901 ttttgataga tcagataata gatcattcct gcaagaacaa ggaagaaaga cagaaagaag
     3961 aaaaagaccc aacggcgaaa actctcagtt ctcggtaaaa aaacgcttgt cggttctgcg
     4021 atacgtaaaa tggcttctaa ctggccactt tttttgactg gtaatgcgac atataaaaga
     4081 tcctcattca aagtagtact tttgcgcaaa gcgctaccta ggttcccacc attcaatacg
     4141 gctttgattt cgggacggga gtttcgctgt tcattcaatg tttcattgtt tgtgtcaaat
     4201 aagatctttc cagtagcatc cattaaagta atccgttcgt tcgactgatg gacgaattct
     4261 tccaaagcag ttttgttttg cgctgcttgg aggttttcga catctaattg ccggatcagc
     4321 agttgtgctt tttttgtcag gtagctttct tgctgttcca cgatctgctg ttgaaaataa
     4381 tgggaaatca attgccagcc accaaaaaac aaactaacta aaagaataag aaagaagaga
     4441 cgttcataaa tttttttcca tttcatcgtt tgggttcctg aaacttatag ccaaatcctc
     4501 taatagtcac caagtacttt gggtgttttg gatctcgctc gattttttct cgcaaatgac
     4561 taacgtgtac atctacgatt cggctttgtc ctgcgaagtc aaaattccaa atgcggtcta
     4621 gcaatgtgtc tcggtcgatc acacggtctt ttcgtttcat gaaataaaca agtaattcaa
     4681 attctttcgg tgttagttca atcagatggt cgtctacggt cacttgataa ttggtcaaat
     4741 cagcagtgat ttgtccaatc gatagatatt caggctcagc ttcatctttt gattgcttac
     4801 gaggttcaat tcgtctgaat attgctttca tacgggctaa tacttcgcga ggactaaatg
     4861 gtttggtcaa atagtcgtca gcacctattt ctaggccaat gatccgatcc acttggtcat
     4921 ctttagccgt cagcatcaaa atcggtgttt catttttttc ttgacgcagc cgctgagtga
     4981 tctccatgcc atccatcgtt ggaagcatca catctaaaat gatgaaatca aatgattgtt
     5041 ctaatgctaa ttctaatcct tcgcctccat ctgtggcact ggttaccttg tagccttctt
     5101 tttccaaatt gaacgtcaag agcgttacaa tagaaggttc atcatcaaca actagtattt
     5161 ttttcattca aatctccttt aaataggttt cattctgttt atattttagc atagaatcag
     5221 tacaaagggc tattttacaa aagaaataaa tggtataata ggaaggcact ttgctgaata
     5281 gaaaggaaga gcagtcaatg attggaatca gtgcatgttt aggcggtctg gcctgtcgct
     5341 atgatggaca gaaaaaagaa gtaccagaat tgatggaact agttgaaact ggagaagctg
     5401 tcatggtctg tccagaagtg atcggcggat taccaattcc tagagatccc gctgaaatca
     5461 ttggtggaaa tggattcgat gtgtggaaag atcaagcaaa agtatggacg acaagcggcg
     5521 aagatgtaac ggaagcgtac aaaaatggcg cgatcaaagc ctatcaaaaa ttgcaggaaa
     5581 aacacattga tctagtcatc atgaaggaaa aaagcccttc atgcggaacc cattcgattt
     5641 atgacggaag tttttccgga ataaaaaaaa atggtgccgg agtggctact gcttatttta
     5701 ttcagcaaaa aatgactgtg ctctctgatg aagaatggct gaaaactaga ggtgagcaga
     5761 atggaaatcg aaaagaccaa tcgaatgaac gcgctctttg aattttattc gaccttattg
     5821 acagaaaaac aaatgaacta catggaatta tattatgcag atgatttttc attaggtgag
     5881 atcgcggaag aatacgaagt aagccgacaa gcagtatatg acaatatcaa gcggacaagc
     5941 aagatcttgg aggattatga aaaaaaactc catctttttt ctaattatat tgttcgagaa
     6001 caggtattgg aaaatatgac tgcatatatt acagataagt acccggaaga taaaaaacta
     6061 cttgaatatg tacagcaaat tcaagcaatc gaagaataaa taaaaggaat gaagaaaata
     6121 tggcatttga aagcttaact gaacgtctcc agcaggcgat gggaaaaata agaaaaaaag
     6181 gaaaagtatc agaagctgac gtaaaagaaa tgatgcggga aatccgcctt gccttactag
     6241 aagccgatgt taacttgcaa gtagtcaaag attttactaa gcgcgtacgc gaacgtgcgg
     6301 taggggcaga agtgctggaa agtttatcac ctgcacaaca aatcgtaaaa atcgttgatg
     6361 aagaattgac gatcacactt ggttctgaaa cagcagagtt aaataaatca ccaaaaatac
     6421 caactgttat catgatggct ggtcttcaag gggcaggtaa aacgaccttt actggtaaat
     6481 tagccaatta tttaaagaaa aatgaaaatg ctcgtccgct cctaatcgca ggggacgtat
     6541 atcgaccagc tgcgatcgac caattgaaag tattaggtca acagctagac gttcctgtat
     6601 ttgatatggg aactgaagtc agcccggtag agatcgtccg tcaagggatg gagttagcaa
     6661 aagaaaagaa aaacgactat gtattgatcg atacggcagg acgtctgcat atcgatgaaa
     6721 cattgatgga tgagttgaaa caaatcaaag aattgacgca accaaatgag attttgcttg
     6781 ttgtcgatgc catgactggt caagatgccg tcaacgtagc tgatagcttc aaccagcaat
     6841 taggtattac aggggtcgtc atcactaaac ttgatgggga tactcgaggc ggggcagcac
     6901 tatcgatccg ctcagtgaca ggcgcaccaa tcaaattcat cggttctggt gaaaaattga
     6961 ctgacttaga agtattccac ccagatcgta tggctagccg tatccttggt atgggggata
     7021 tgctgacatt gattgaaaaa gcgcagcaag actacgacga gaaaaaagcg gaagaacttg
     7081 cacagaaaat gcgggaaaat tcattcgatt tcaatgactt tattgaacag cttgatcaag
     7141 tgatgggtat gggcccactg gaagatctaa tcaaaatgat cccaggaatg aatcaagtcc
     7201 ctggaatcga aaacgtcaag gtagatccaa aagatgttga acgcaaaaaa gcgatggttt
     7261 attctatgac gccagcagaa agagcaaatc ctgatctgtt gaacccaagc cgtcgtcgcc
     7321 gaattgctgc cggttctgga aacagtgttg ttgaagtgaa tcgaatgatc aaacaattca
     7381 aggaatcaag aaaaatgatg cagcaaatgt ccaaagggga tatgaacatc ccaggtatgg
     7441 accaaatgtt tggttcaggc gtcaaaggaa aactaggcaa aatggcgatg aatcgaatga
     7501 tcaagaaaaa taagaagaaa aagaaaaaac ggaaataaga tgaaaaaaac agcttcggta
     7561 cagactgaaa atgtctactg ccggagcttt tctatcagca atttttattc tttactaata
     7621 caattactct aaacgaagag gtggcacaga tgagatgtcc gtggagtgaa caaacagaaa
     7681 gtatgcggca atatcatgat actgtctggg gtgttcctga atatgatgac caaaagctgt
     7741 tccgaaaatt gatgcttgat attaatcaag ccggattgag ctggcagacg attttaaata
     7801 aaagtgaagc attcgatgaa gcatatgatc gattcgatat caaaaaagtc gcaaattatg
     7861 gagaaacaaa aatcagtgaa ctaatggaaa acaaaggaat tattcgtaac cgaagaaaaa
     7921 tcgaagcagc cgtccaaaac gccaaagcag tcttgaaaat tcaagaagaa atcggcagtt
     7981 tttcagagta tctttggtcg ttttctaatg gaaaaatcgt tgattcttct tatcattcac
     8041 aagaagaagt cccaacaaca agtcctcttt cagatgaatt agcaaaagat ctaaagaaaa
     8101 gaggatttaa atttatcgga agcacaacaa tctatgcttt tttagaagct gtcgggatca
     8161 tcaatgatca ccttgacagt tgtttccgca aagaagaggt cgtacgtatc ggcagaaagg
     8221 gcgaataaag catgcgaaaa atcatatttt atggtgctat aagtttagat ggctatttgg
     8281 caacgaaaac ggatgatctt caatggcttt ttgacactcc aacaggtaag caaactactt
     8341 atgaagcttt ttatgaaaca atcgatacga ccatcatggg cagaaaaact tatcaggaag
     8401 caaaaaaata tttggagacc gaagcaattt atccagagaa aaagaatttc gttttttcca
     8461 cccacgcaca attacaattg ccagatgctg aggtcgtaaa cgaagatcct gttgcttttc
     8521 taaaaaatct acaaatgaca gaaggaaaag atatctgggt tgtgggtggc ggtcgattga
     8581 tcaaaccgct attggaacat caaatgattg atgaatggta tatccagatt acccctgtat
     8641 tattagggga cgggattcct ttattccaaa aaggaacata tgaagcgcgt tttgaaatgc
     8701 tagacacgac acgattcggt gaatttatcg aattgcatta tgtacgaaag tagaagtaaa
     8761 taaaataaaa aaggcaacta tatgcagaaa tgtcctgtac atagttgcct tttttattac
     8821 cgttatgctt tttactctgt taatttgtta gaaaaatcta gatacgcatc taaaatcgtt
     8881 tggaagaaac ttaatgttcc ttcaacgata ttaccatttt catcaattag attcgcaacg
     8941 tttccgatat atgcttccgg ttgctgcaag gttggcacat tcaaaaagac aagtgattgg
     9001 cgtagatggt ggtttgcgcc aaagccgccg atcccacctg gtgatgcagt aacgactaaa
     9061 cctggtttat gatcccaaac actgtgtcca taaggtcttg agcctacatc taaagcattt
     9121 ttcaatactg ctggcacaga acggttgtat tcaggagtaa cgaaaacgac accgtctagt
     9181 cccctcactt cctcacgaaa acgagtccat tcagcaggag cctgttctgg agtttcgaga
     9241 tcctcattgt aaaatggcag atcatcgatt tttacaaata cactttcgta cccttcagga
     9301 agcaatttcg caaaagcttc tgctactttt ttgttgtaag aatcttttct taaactacca
     9361 ataaaaaatc caattttttt tgtcatacga tttttcctcc ttcaaactat ctataataat
     9421 cttacaaaag ataagtaact ggagcaaata aaaacactta ctgacaaaag ctacatagaa
     9481 gatagattac ttatttttaa caaaaaagaa gtctaaaatc ttctattttc taaaaaaaat
     9541 cgaatttaat caagctgtaa agaaaaaaag ctttacaggg taaaaaaaaa ctgttacact
     9601 acatcttgtg aataaaacaa tggaggtgga aataaataat ggcagttaaa atccgtttaa
     9661 aacgtatggg ttctaaaaag agtccgtttt accgtatcgt cgtagctgat tcacgttctc
     9721 ctcgtgatgg acgtttcatc gaaactgtag gtacttacaa tcctttgaaa gaccctgcag
     9781 aagtagtttt aaaagaagac ttagttcttg actggttatc aaaaggtgca caaccttctg
     9841 acactgtacg taacatcctt tcaaaagaag gcgttatgca aaaacaccac gaagctaaat
     9901 tctcaaagaa ataaggtgac cggatatgaa agatttaagc gacttagtct taacaatcgt
     9961 tcgtccgcta gtcacttatc ctgatcaagt caagttagac gtcgtagagt cgaaagactt
    10021 ttacgagtac aatctaacag tggctcctga agatattggc cgtatcattg gaaaacaagg
    10081 gcgtgttgct aaagcaatcc gcacaatcgt ttacggtgtt cggatcaatg caccgaaaaa
    10141 agtgcgtttg aatatcatcg atgataagga aaagtaaata aacgtgctgt actcgaattt
    10201 tcgagtgcag cttttttgtt ttttttatta attgaagaaa tgattatttg gcaaaacaag
    10261 caagaaagcg tatccttata ggaggaggga gtgaacagcc aatgagaaag aaaatgttat
    10321 ttattccatt tgctctattg ttaagcgctt gtagttcggt tactcaagaa tcagacaata
    10381 cacatctatc aagcagtata tctgaaactg ttaccagtcc agaaaaaagt agtaccaaaa
    10441 cggcttccac cactgaacaa acaatggcta cctcaaataa tgagaaaaag actgctttag
    10501 accagttgaa agaacaacag ccaaatgttc ccatgcctct agatgtacca gtctcttcag
    10561 gctatctcaa tatcgcagcg acacatacaa agcaaggtta ttctatttta tattacagaa
    10621 cagatcgtcc cctcgggttg aatgctgacg aattgaatca agaaacgcct attgcaacct
    10681 atctatatca atatgggttt gcttctagtc aagaaacaat ccaagttttg cagccatttg
    10741 agatcgacac aaatggtcag caagtggact taggttctcg aatcacaggg tatcagcaag
    10801 gtgcagcagg ttcaagtttt cttgaatggc aagaaggaaa ctggcgtatt cgaatccgtg
    10861 ggaataatat agaaggccaa gatcccttac tattagctaa agaaatcgta gcttatttgg
    10921 aagaaaacag tctaccagca cctgaacaat ttggtaaaat tactgttgat atgggtgata
    10981 cgacgaatcg agccgtagaa gtaagctggc aagaaccgaa aaatgcctat acgatcactc
    11041 atcaagatcc tatgtctgcg ttgaagatgg cagtttctat gaagaggttg tgaaatcagc
    11101 gcgctgatct gagcccatca tttaactcca agtcacagga agtaatcaaa aatagcacgc
    11161 cgcatttttg cataattttg gcttaatacc ttaaaagaaa actagaattg cctcttcaca
    11221 catgctaaaa taaaacagac aaaaaagaaa tggggatata agttgactga atacttgaat
    11281 gtaggaaaaa tcgtaaacac acaaggtatc aaaggcgaag tccgtgttat ctcaacaacg
    11341 gatttcccag aagaacgtta caaaaaagga acggtgctaa cactattcca agacggaaaa
    11401 gcacctgtgg aattaacagt caaaagtcat cgcaaacata aaaattttga cttattgagt
    11461 tttgaaggac atccttctat caatgatgtg gaaaaatatc gggatggcat attaaaagtg
    11521 tcaaaagaca actctgtaga tctagctgaa aatgaattct attaccatga gatcatcggt
    11581 ctgcatgtat tagaagatgg aactgagtta ggtaaagtaa aagaaatctt gtcaccaggt
    11641 gccaatgatg tttgggtcgt tcaacgtccg aaaaaagctg atatattgct tccatatatc
    11701 gcttctgtcg tcaaagaagt cgatttagaa gctggagtgg tccgtgtaga aattccagaa
    11761 ggactgattg acgatgaaga ttgatgtatt gactttattt ccaagaatgt ttgaaggtcc
    11821 tatgggggaa tccatcatcg gtaaggctgt agataaagga ttattagata tcaatatctc
    11881 taacttccgc gattattcag ataacaaaca tcagacagta gatgattatc catacggcgg
    11941 tggagcaggt atgctgctca aagtccagcc catctatgat aacatccaag ccattgaaaa
    12001 agctgcaccg gatgtcaaaa aaagagtaat tcttcttgac ccagctggta aacgattcga
    12061 tcagaaaatg gcagaagaat tttcacaaga agaacactta gtattcattt gtggtcatta
    12121 tgagggatat gatgaacgta tccgcacatt agtgacagat gaagtttcct taggagatta
    12181 tgtccttact gggggagaat taggtgcgat ggtcatgatc gatgcaacag ttcggctttt
    12241 gccagatgtg ttagggaatc agacttcagc tcaaacggat tcttattcaa ccggtttatt
    12301 ggaacatccc caatatactc gcccggcaga atataaaggg atgaaagtac cagaagtatt
    12361 gacaaatgga aatcataaat tgattgaaga atggcaatta aaagaatcac taagaaggac
    12421 ctatcaacgt cggcctgatc ttttggaaac acttgaatgg acacctcaaa tgacgaaatt
    12481 tttagaagaa atcaagaaag aagaacaaga aaaaacagca gatgaagcca agtgactttt
    12541 tctaaaatct tctttacagt aggttgataa tatgttagta ttttatggtg agtgcaaaag
    12601 cacttaacta ttacgatatt ccgctgtgag aaaagctcat aagaatattt ggaataagga
    12661 gaagaaaacc atgaatccat taatcgaaga attaacaaaa gaacaattac gttctgacat
    12721 cccagcgttc cgccctggtg acactgtgcg tgttcacgca aaagttgttg aaggtactcg
    12781 tgaacgtatc cagttatttg aaggtgttgt tatcaaacgc cgcggtgctg gaatcagcga
    12841 aacttataca gtacgtaaag tttctaacgg agtgggcgtt gaacgtacat tcccattgca
    12901 tacaccacgt gttgcaaaaa tcgaagtagt tcgctacggt aaagtacgtc gtgcaaaatt
    12961 gtactaccta cgtgcattac acggaaaagc tgctcgtatc aaagaaatcc gtcgttaatc
    13021 attgttatca gactgagaaa gaacccttgg tattcaaggg ttcttttttc ttagcatgta
    13081 atttgaacac cctgatccag ttttttcttt aattattggt aggggagaat aatggattag
    13141 aataaatcta cctataagta gtagtaaaaa aaagagcact atgataatat ctttcttaag
    13201 agtaaccgct ttaataaaaa atccagaata ttctaacata aatggagtat tcttagaatc
    13261 aaaaggagaa agaagatggt ttatacttat acggagcaag aaaagatgaa agttttagac
    13321 acattcatga atgatggaaa agtaaaacaa gtaccaagtt caacaaaaaa gaaatacatc
    13381 ctattgaaag aagtcttgaa acgctttgac catggagtga cttatacaga aacagaggtt
    13441 aattcgatac tgctgaacgt attttctagt ggcgattatg tcgaacaaag acgttatcta
    13501 atcacatttg gctttttcaa aagaagttca gacggtcggg catatcagat gatgggaatt
    13561 gaaaattaga agttttaatt tatactcatt gaaaaaagtt caagtatgcg atattggttc
    13621 tacaccgcat acttgaactt tttttgtcat ctgtatctat aatacaaagc caagaacgtt
    13681 attcaaaata tggatagcta tgataaaagc taatgagcga ttcgcagtta aataagcaat
    13741 cgtcagtaaa atcgttccag gagcgtacat gaaaaaatca attggatgcg ttacatgaat
    13801 aaagataatt ctcttttatt tgtacgaata aataccaaaa cgtacctcct aaaattagat
    13861 tttggacaga tgccctttaa aaagataaca agatttcgtt cttgaaacaa tatatatgct
    13921 tttattccaa tgaggttgtg aaaacagtat tttgtcctga gcgttagaaa attgaaaatg
    13981 gggaatagca actcgtactt taccattttt taagtgaata caagatcagc taattgaaca
    14041 ccgtttattc gtgatgaggg acacaacact gcagtcgaca catttctctt ttatgttcaa
    14101 agataacaaa tatttaaaac aaagaaaaat agtagataat cccgtcctaa ctcttttatt
    14161 cttccctcat agcttccaaa tttaagcaat acttcaatta atcaagtttt atcttttttt
    14221 ttcaataatc gtaaaaaaag agtatttttt atttaaaaat aaaatctttg tattaaagga
    14281 caagactaaa ataaccttag aataaaaaga gaaaaaaagc cccaaacttt ctatacatca
    14341 ataaaataat aaaaattaaa tttagaataa cgtgtaaagg attatttgat gattttttat
    14401 tgagaggagg gatgaaaatg attaagaaaa ctcaaaataa aatccaacgt atatgtcaaa
    14461 aacattttgt ttctagactc aaagctgtat ctaaaagaaa atacttacat cccattatga
    14521 tttcgatcat cttgattttt actttcttag ggagcttatc aagtatttat gtaaacgttt
    14581 atacagaaac gtcaaatttt gctaaccaaa cgacagagga gacaaatagt ccaccctatt
    14641 ctgaaataca gtccatagaa agcaccaatc agtctagcaa caacgagaca acctcaacca
    14701 catcctcgac tcaactgatt gatccgacag aaaaagaaat cacagcagca aaagcagcag
    14761 gggagaaagc ttattcagaa acaggtagaa gccagacatt tagtactgtt gcggccactg
    14821 cgaatttcaa cggacatacc ataccagatg gatatacagt gatatatgcc aaagatggag
    14881 tcattgttgc ggttaacgga tcgatgagtg cggataaaac tgcgttacaa ggcgtaacca
    14941 aagcaatcgc ttttattgat aaccgtccag atatccataa tctgtttatt ggctccacag
    15001 taaatggtac tactatcaca ttcccaaaaa gtatctatat gggggtggct accgctggaa
    15061 atacgatcga ttcagttaca cccaatgcgg ataaaagtta ttcttttaca ccctcaacat
    15121 ggggagggct tcctgccgga ggaggtatga gtactttttc aatcggtata cagttagcaa
    15181 atgatattcc attcacgggg tactcgatca aaaatgggca gatcgttgat ggtaagggaa
    15241 atgtttcaaa ttttccaacc tatcaagcag caacggttca ggtcaatgcg aatcggacag
    15301 gaactttttc ttatacagtc aatggtggag cagcaaaaat cggaacgtac gggactacat
    15361 tatctggaat ccatgtgggt gacaaagtgg ttatcactcc aacagcagta acgggctatt
    15421 cttttgcaca aacaaacggt tctttcacag ccctagaaag taacgtcgat acggtgacct
    15481 atacaggcaa acaaacacca atcactatta agtatcaaga ctattttgga cagatgattg
    15541 ctccttctaa aataatgaat cttacttacg gaagtgctgc acaagacctg acgactaata
    15601 cgcctactat caacggatat acctttacag ctgtttcagc gacagaaaca aagaaccaat
    15661 cagcaatgag tgttttagct agtttagata caaatggaaa tgtaatcgta aaggacgcaa
    15721 atggaaaaca gatttctgaa gtcatcctat attataagac aaatgcaaca gtatcaatca
    15781 atgcaaatgg cagtaagtac tatgatggtc tttctgttgt accgatcgtt aaatatgcct
    15841 ttaatgataa aacagaatca acatcccttt taaacaatga tctcagtatt gatcaaatca
    15901 catggaatcc aactgatttc aaagcgatga atgaaaaggg agttgaagta actgcgccta
    15961 ctgaaattgg aacctatacg tcttggcaat tgacaaaaag cggactagct aaactagctg
    16021 ccagaacaaa ctatttattt actatcgtgc agacatctga tgtctatacg attaagcaaa
    16081 tcagcggtgc ggtagagctc agtggcagta agacatatga tggaaaagca ggtgtcccgt
    16141 caatccatgt caagctagca gagggtgtca ctagttcgct tactccagta caagtggcac
    16201 ttagcagtat ggattacaca gcagatgcac aatcagcaaa aaatatggtt aatgctggca
    16261 gctatacaat taaactaacg aatagcggaa tagacaaagt caaagcagca aatcccaagt
    16321 tgtcatttac tgatatagcc aacacatcag gaacttatac catctataaa gctaacgcag
    16381 tgattactgt cgatgacgct gcctttaatt atgatgccca aagtcactcg atttcggttg
    16441 gaaatctcca cgttaccggt gttgtcttag gcgaatcatt ggattatacc ctaacagtta
    16501 acagccggac agatattgga tcacaaatag taacgattag tttaggcgaa ggaagtgtaa
    16561 acaataatta tacgattatc gcaacagata ccgctaagtt gacgatcaat ccgactctct
    16621 tgcttccttc gacaggtggg ataggtctac tgccctttgt ctttttagga ctgatcttta
    16681 ttttttctgg cttcttttat tttatccatc gtaagaaaaa agcaggtgaa cagcgatgaa
    16741 aaaaacaaaa aatgtacgtg tggatatatt tgctgttctc ttttttatcc tactggtctt
    16801 ttttataggt tctccgactg tgtcagctag agaatcacct attacattta ctttgcataa
    16861 tcgaatgttc gaaagcgata agatgccaag tatcagtcaa aacgacggaa taaatatccc
    16921 ggataacttt caaggagtaa aagggttgaa cggcgttact ttcgctatat atgatgtctc
    16981 ggatgaattt tacaagctgc ggtcagaagg aagtagtgtg gaagacgcac aaagaaaact
    17041 agcacaaaaa agtgatagtg aaaaaatact ggcagaaaat gtgacaaaaa cagtagacga
    17101 ggaagagggc attgcttctt ttagtgcatc ggacaaagat gagcaaggtc gtgacgctgt
    17161 ttaccgattt gcggaaataa agacttcaga ttaagtaaaa gaaaaatcag caccatttgt
    17221 cgttgtgctt cctgtgacag attcatctaa tcataaattg acgaccatcc acttgtatcc
    17281 aaagagcgaa caaaaaatac ctgctgcact cacattaaca aaaacagtag agaataagca
    17341 gacagatttt gcagatgggg ataaagttcc ttatctcatc accgtaacaa ttcctgagaa
    17401 catcaacgag ataggaacct atacgatcaa agatacagct gatcctcagt tatggctaga
    17461 accagaaacg atcgattttt tgattggtgg agacaagatt catacatttc acacacaaaa
    17521 aacaaaacat ggttttgtct tatcagattc tggaaaagat cttagttcgt ttgctgggaa
    17581 aaaattaacg atccgttacc aaatgacatt gaaagaaaac agtgaaaaaa cgacatttga
    17641 taacgatgta cgtttgacga ctgacaatca aacagtagaa acacatcttg ccatccaaac
    17701 aggaggcaaa caatttgtca aagtggatcg gcaaacgaaa caaaaactag cagatgctca
    17761 atttctagtc aaaaaaaatg accaatactt agcgcaagaa aacgggataa atcattgggt
    17821 agccaaagat gattcaagag caacgatttt tacttccagt aaagatggaa cattttctgt
    17881 ccatggattg tcttttggaa gttacgaact agtggagatt aaaccgccta aaggatatat
    17941 attgagtcaa tcaaccattc tctttgaagt tgaaaaaggc agttatgatc cgcttcatcc
    18001 tataccattg gaaatcatca acgaaccaaa agcaaatccg acgaataatg gaaaaacgcc
    18061 agttcagatg cagacaaatg gtccatcaaa tgaaaaaaat ggaggaagct ttccaaaaac
    18121 aagcgaagag atgcttggag gtttctcaat tttagggttg ttgctagtcc tttcaacagg
    18181 gacagcttgg ttttataaaa aacaacaaaa aggaaacaat aggagggaaa taaaatgaaa
    18241 aacatcaaaa caaaaatatg gacgttttta ggaactgcta tcatgctgct tcctttcgtt
    18301 ctcggcctag gaactgcaga ggtttctagg ccgtatcacc tacaccagaa aatgttacgg
    18361 ttaatttgca taaattgaaa ttcacttcag caccagaaaa tcaaataaac aacggaacgg
    18421 aattgacatt tccgaatagc gaaccgttga acggcgttga attcaacgta tatgacatca
    18481 ctgctacgta ctatccaagt aaagacacag cagttccagc agacgctaca cccttcgcaa
    18541 gtgtaacgac ttctggtgaa ggattagcta acctgactct tccaggtaaa tctgatggga
    18601 aagatgctgt atacgtcttt gtagaaacac caaagcccgg tgtcgaaacg tcgccaaata
    18661 tcgtgttaag cttacccttt tataacccga attcatcaga ccctgacaaa cctttagaca
    18721 cgattcacct atatgctaaa aatgtcatca aagaaagcaa tatcaaaatc cagaaagctg
    18781 ggaacttttc aggggtagat aagttagcag gggcgcaatt ccagatcaaa aattcagaga
    18841 atcaattcgt gaccggtttt gatgaaaaca ctggtgtcgc tctctatgat cctgctgagt
    18901 cagcgttgac attcacaact gatactgaag gatccatcag tgtcaatgga ttaatagacg
    18961 gcaattatac tttagttgaa acaaaagcac cagatggtta tcaattaacc aatccacaaa
    19021 aagaaactac ttttatagta aaaaacggaa aattgcaaca aacaaccggt atcatccatg
    19081 gcgcagatga agccaatctt gggaatccaa acggtgtagt caatcgggaa aatacaacta
    19141 cggataacac gattactgta gaaaatgtcc aaaactacgg tgatttccac ttcatcaaac
    19201 aagatgtgaa cacgcaagaa aaattagccg atgcagaatt ttctattcta agagcaaaag
    19261 atccaactgt taaattaatg aaaaaaatag caaatcttgc ctcaggagaa tacccatatg
    19321 cttggtcaga tgaagtcaca ggttctgact gggaagaagt gaaattgatt tctgattcag
    19381 aagggaaatt ttcaatcgaa gggctgaaac aaggggcata ctgccttcaa gaaacaaaag
    19441 caccagaagg ctacgcatta cctaagaatg aagctgcgta cactgaattt acagtagatg
    19501 atgatccaac aacttctgat gaaacaacga tcgataacca acctaaagga atcttgcctt
    19561 caactggtgg tatggggatc atcgtattca tcttggtagg tactgcttta gttggaggag
    19621 cagtgatcta cttcaaaaaa cgccatcaag aaacagaagc ttaaactatt ggaaacaaaa
    19681 atcatacgat aaataaacga tagtgattca aatggggaga aaggttgtga caagtggtcg
    19741 gtc
//
LOCUS       DACCQI010000059        19225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCQI010000059 DACCQI010000000
VERSION     DACCQI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.060"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IVO76_001629"
     CDS             168..362
                     /locus_tag="IVO76_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429593.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IVO76_001630"
     CDS             631..930
                     /locus_tag="IVO76_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429594.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IVO76_001631"
     CDS             complement(1027..1461)
                     /locus_tag="IVO76_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7429595.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IVO76_001632"
     CDS             complement(1693..2403)
                     /locus_tag="IVO76_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7429596.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IVO76_001633"
     CDS             complement(2393..3253)
                     /locus_tag="IVO76_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7429597.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IVO76_001634"
     CDS             complement(3258..3896)
                     /locus_tag="IVO76_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7429598.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IVO76_001635"
     CDS             complement(3911..4210)
                     /locus_tag="IVO76_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7429599.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IVO76_001636"
     CDS             complement(4253..5737)
                     /locus_tag="IVO76_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7429600.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IVO76_001637"
     CDS             complement(5757..6218)
                     /locus_tag="IVO76_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7429601.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVO76_001638"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVO76_001638"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7429602.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IVO76_001639"
     CDS             7521..8291
                     /locus_tag="IVO76_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7429603.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IVO76_001640"
     CDS             complement(8564..9391)
                     /locus_tag="IVO76_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7429604.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IVO76_001641"
     CDS             9959..11194
                     /locus_tag="IVO76_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7429605.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IVO76_001642"
     CDS             complement(12193..12453)
                     /locus_tag="IVO76_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7429606.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IVO76_001643"
     CDS             complement(12480..13781)
                     /locus_tag="IVO76_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7429607.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IVO76_001644"
     CDS             complement(14073..14531)
                     /locus_tag="IVO76_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7429608.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IVO76_001645"
     CDS             complement(14544..15869)
                     /locus_tag="IVO76_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7429609.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IVO76_001646"
     CDS             complement(15886..17298)
                     /locus_tag="IVO76_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7429610.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IVO76_001647"
     CDS             complement(17315..18079)
                     /locus_tag="IVO76_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7429611.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IVO76_001648"
     CDS             complement(18183..19058)
                     /locus_tag="IVO76_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7429612.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         6116 a         3952 c         2947 g         6210 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tattctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 ccttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa cctgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taata
//
LOCUS       DACCQI010000060        18889 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCQI010000060 DACCQI010000000
VERSION     DACCQI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.061"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..357)
                     /locus_tag="IVO76_001649"
     CDS             complement(<1..357)
                     /locus_tag="IVO76_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429613.1"
                     /translation="MLTKKELIDHIDETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIIDILDLKVDPELGGILRGKEGYFPAYHMNKEHWISVDLRKI
                     EKLDEPDCRQKLSVRTA"
     gene            501..1940
                     /locus_tag="IVO76_001650"
                     /pseudo
     CDS             501..1940
                     /locus_tag="IVO76_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
     gene            complement(1947..4499)
                     /locus_tag="IVO76_001651"
     CDS             complement(1947..4499)
                     /locus_tag="IVO76_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP7429614.1"
                     /translation="MNWYKLTVEDTLKNYQTSTEGLSQEERQKRLTDQGYNEIEARQQ
                     TKRWKKIVKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADVRLIEVHDLLVE
                     ESILTGESEASEKNHTVLAAEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLILFLVVFTTFSYGMDWPILLSAMIAL
                     VVSMVPEGLPAVLTMILSVGVNEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEETRVLSNMKNCQELKTKEEQKTENLSGNPTELALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHTEGKGAVIYVKGAPEVLLAKSTLSSSEQEAWGQTATAFAKK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSEEELIRQVPKVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRADIGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILILALILNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVALLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKSLFLSLGILALLQGMVIFLPS
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREFSR"
     gene            complement(4612..5943)
                     /locus_tag="IVO76_001652"
     CDS             complement(4612..5943)
                     /locus_tag="IVO76_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7429615.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIALAGPVVI
                     LVYLICGVAIYGLMRAMGDLFLANSKFNSISDFIDYYLGEKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIV
                     FFILLGIGLSVSYFHTQSIGIKANFGQLNELLPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYVGTILSILFVVPWHCLDLKESPLVTMLQMIH
                     CQSGAAIVNLMILVSSLSSANSAVYSTSRILYQTAKEKSLPPWFCALSKKGVPLHGIL
                     ITGTLFLAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHIQFVKEKKRNTIP
                     KYKDLALLVFFVAIAFSLLFERMTRFIPFLLVIWFVLLSFVYKRISDRNIN"
     gene            complement(5909..7387)
                     /locus_tag="IVO76_001653"
     CDS             complement(5909..7387)
                     /locus_tag="IVO76_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7429616.1"
                     /translation="MKDAGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFIVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLTFDMAKIGGKIGVWLGLY
                     VPLTMMLLLGLAAWIKVGIVPTGTLGNFSWDKVIPDSTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGISVLRLRY
                     THAKLNRPFRIGKKENKSNLGVWAIVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(7694..9529)
                     /gene="tdc"
                     /locus_tag="IVO76_001654"
     CDS             complement(7694..9529)
                     /gene="tdc"
                     /locus_tag="IVO76_001654"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7429617.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMADGWGHITADGSIANLEGLW
                     YARCIKSIPLAVKEILPEKVKNMSEWALLNLSVEEILEMTESFTDEEMDKVKAASSRR
                     GKNIQKLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     DQKIPILGVVAVVGTTEEGQVDSVDKIIQLREKLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHYVTIDKEVYEGFRAISEADSITIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVEAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLEQLHFTVKGKTIEVYPLNHPDFNMVNWVFKVQDC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFIERMGLSKEE
                     WQKEQQITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIK"
     gene            9999..11426
                     /locus_tag="IVO76_001655"
     CDS             9999..11426
                     /locus_tag="IVO76_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7429618.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIVYTHREKDCYTLYFSEHIPRCKIMQQLTQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFHDCGIEIDRYSPRFTEMERRLLLLNVAYRL
                     GSFKSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQLGTVTID
                     PTFIEEIKKRPIYNYVESTWETTDFQTYYKKEEFVFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFETHFRQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQANLYKDVQTLFSSWAKRLPYDLRFNPNCMRAFVIELSGILRLTKERLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYASISDIKKYAQQPSNRVLCERTLYTPDA
                     VQYENIIPISINTIERAIISAVQNK"
     gene            complement(11587..11952)
                     /locus_tag="IVO76_001656"
     CDS             complement(11587..11952)
                     /locus_tag="IVO76_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7429619.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLIWGVCLSIKDFLKYLF
                     VHTEAKNDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVPNK"
     gene            complement(11949..12314)
                     /locus_tag="IVO76_001657"
     CDS             complement(11949..12314)
                     /locus_tag="IVO76_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP7429620.1"
                     /translation="MFHSNLIEFLEWIIAILNICSIGILVAGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEIG"
     gene            12755..12883
                     /locus_tag="IVO76_001658"
     CDS             12755..12883
                     /locus_tag="IVO76_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP7429621.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            12940..14634
                     /locus_tag="IVO76_001659"
     CDS             12940..14634
                     /locus_tag="IVO76_001659"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP7429622.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKDTPMQDEITVTGLKMTNTLTQ
                     EEEHVTVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            complement(15038..15898)
                     /locus_tag="IVO76_001660"
     CDS             complement(15038..15898)
                     /locus_tag="IVO76_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429623.1"
                     /translation="MKQIQNKWNKRIIVMISSVCLFCLTVMLCLISTLANQHFMLEQN
                     MQSDYAKKVAAEINREIQSYHYEIGAPAEALEKIVSEELVQKNIELFIRNRYEKEPVE
                     LIGEKEVEEKLQEIINTYKQEEAEDDPFASEENNLLIYMTSGIFRRGIQSIYLMSFID
                     QILTIRERMVSFLPMISSILLVLLSGVFYLYRMSWKKVLWSFAVVIGGAGFQLLSLSV
                     SLYLYDMASQLDIRTQALEHLLDTYILSFIQFFSVVAIVEVVIAAGLWWIGKRVNTEK
                     KVFSKRNGYK"
     gene            16189..17322
                     /locus_tag="IVO76_001661"
     CDS             16189..17322
                     /locus_tag="IVO76_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP7429624.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPRILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDETNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNNLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLEEAAQAYEIFDKKEDKNIKVVLKP"
     gene            17353..17667
                     /locus_tag="IVO76_001662"
     CDS             17353..17667
                     /locus_tag="IVO76_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429625.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAVFLTIRPILRIVRNIFIYDEKRTRKKHGK
                     AV"
     gene            complement(17804..18262)
                     /locus_tag="IVO76_001663"
     CDS             complement(17804..18262)
                     /locus_tag="IVO76_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7429626.1"
                     /translation="MERRTVIKLRDRPTLIKETAEWFASKWHLPVAVYEESIQASTRK
                     NTSVPQWYVVLNEWGEIIAGAGVVDNDFHDRVDLTPNVCALFVEKDYRNQRIAKMILE
                     TIKQDFAEMGVKVLYLVTDHTDFYEKYNWQFIGMAQDNEGIPVRVYQMTL"
     gene            complement(18484..>18889)
                     /locus_tag="IVO76_001664"
     CDS             complement(18484..>18889)
                     /locus_tag="IVO76_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7429627.1"
                     /translation="ATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPI
                     VLNPIMFIPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPVIGGVLATKSIAGGV
                     LAAVNLILSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         5869 a         3874 c         3262 g         5884 t
ORIGIN      
        1 agctgtcctc acagacagct tttgtctaca gtctggttca tctagttttt ctattttacg
       61 tagatcaaca gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt
      121 tcctcgcaaa attccgccaa gttcaggatc gaccttcaaa tcaaggatgt cgatgatcct
      181 atggtcactt gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca
      241 ttttttgctt ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc
      301 tggagacaaa ttgtatgttt catcgatgtg atcgattagt tcttttttcg ttagcatctg
      361 cttccatctc tttcttattg attcacttta ttcaaagata ccacgtaaga atagaaaaat
      421 gaatactggt tgaaagatca gttgaaactt gaccaccgtt tattttctcc gtattcttag
      481 aatagaaggg agtctgattt atgtattcac taatgaaaaa aatcattacc gaaaaagata
      541 ttcgacgtca tgtttcttta gtcgaacaat tgctgaatca tacaaagata acggtaaatg
      601 aattagctga aatcataggg acaaccgaac gaacgatttt ttctgacctt caatctattc
      661 gctctcagct gcccgaaggc tgggacatcc tcagcgatca agcaggaata agtttgcaaa
      721 accatcaaaa tttattaaca aatgaccttt gggagatttt tttcaaacaa tcagtcagtg
      781 ttgagctgct gaaaaatctg ctttttacaa aaaaagttgc tgtccctgat tttctagcgg
      841 atcacgggct ttcttatggg acattaaaaa gacatgtgac taaaatcaat cagcgtttag
      901 caagttacga cttgcagatc gatttgacga aatatacagc ttgtattctt ggaaaagagc
      961 gtgcgattcg tactttctat catcgattgc tgattccttt cactcataac aactattttt
     1021 tcgaggatta ttcgatccat gaatcccatt attttcaatt tttaagaaac ctcagtcaaa
     1081 ctgaactagc tgttgaaaca gaagagattt tcggaacatg ttggttcttt atcaatacga
     1141 tccgtataaa agcgaattgc cgattagatt cttctatcca tatcaacagc acactctcct
     1201 ccctgtatga ctctgcattg aaaaaattgt atttaacgga aggtatttat cttaaagaca
     1261 ctgagctttc ttttgcttct ttctgctttc tggaaagttg gaattacaac aataactatg
     1321 gacaagaaat cgctcgctgc ctgcatcatt ctccattttt agaggtcctc gaaacgtttg
     1381 tagaggaact agcttcagag ctgtctcttg atcaactaaa aaaaacatct ctgactgata
     1441 acttggctct cctcgtatta aaatatcatg aatcgccgat actgatttaa cctttggaac
     1501 gccaatacca tcgtttttta gagacgtatg atcagcaata tgcacacttg tatcagcaga
     1561 aagagaattt actgaacgat ctaaaaaaag aaatggaaat caatgaacca aattactttc
     1621 ttcaactctt gtctttactg atccaacaaa cgattttttc tatcaaaccg aatttgttca
     1681 atgtctattt tattttccaa ggagaacctt catggaaggc gttcttgcag caggaattaa
     1741 aagattactt gggaaaacgt gtctgcttct tacctatcga actaaccgat ctttccacta
     1801 tttcttttga aaaaacagat atcttgattt ccaatttccc tctggattct ctagatatcc
     1861 ctattgtata catttcttct attccaacga aaaatgagct caatcgtttg actgaactca
     1921 ctttcaaaac ttttttatag taacggctag cgactgaatt ctctagctgt tttttctttt
     1981 ttgcgaaaca gtttattcat gatccctttt tctatttcta caactaagaa tagaagaatc
     2041 acatttgccc caatcaataa ctgttgtgtc atcgttaaac ttactgtacc gatcagctgc
     2101 tggccgcttg gcagaaaaat aaccatcccc tgcaataaag caaggatacc caatgaaaga
     2161 aaaagcgatt tgttttgcaa aagaccttgg ttcaacgacg gcttggacaa ctctctgcaa
     2221 ttgatcatgt aaacagcttg agctaagacg atattttgca gaagaatggt ttgctgtaag
     2281 ctttgccctt caaattgcat agccaataaa taggcaggaa ccatgatcag tagtgcgaca
     2341 tagatgatcc gaaacaagtg ataacctgat aggattcctt gctttggatc tcttggtgga
     2401 cgcttcatga tttctggatc tggcgactcg aaccctaatg cataagataa agtgattgtc
     2461 gttaccatat tgacccataa aatctgtact ggggacaaag gtagcggccg attcagtatc
     2521 agcgccaaaa tcaagatcaa tccttgagct aacgcagttg gtaagaagaa attgatggtc
     2581 ttttgcaagt tatccaaaat gcgccgacct tcttttacgg cttttgcgat cgtatggaaa
     2641 ttatcatctc ctaaaaccat atcagctgcc tgtttgctta cttcactacc tttgattccc
     2701 atagcgattc ctatatcggc tcttttcaaa gcaggtgcat catttacgcc gtctccggtc
     2761 attccgacga tttctccatt tttctgcaaa gctttcacga ttcgcagttt gtgttctggt
     2821 gtcgttcgag caaagacatc gacttttggt acttgtcgaa tcaactcttc ttcagacata
     2881 agatccaaat cgatcccttc taaaacttta gcagtatgtt ttaagccaat ttgttccgca
     2941 atcgcttttg ctgtgtcttt atgatccccc gtaatcattt tcactgagat ccctgcttgt
     3001 tggcattcct ttacagcctt gactgcactt tcttttggcg gatcgatcaa cccggcaatc
     3061 cctgcaaaag ttaattgggt taaagtctca tgcgtgagtt cctgtttcga atctactttt
     3121 ttgtaagcaa atcctaaaac acgctgacct tttttcgcaa acgctgtggc tgtttgaccc
     3181 catgcttctt gttctgaaga agaaagagtt gatttagcca ataatacttc tggcgcacct
     3241 ttgacgtaaa tcaccgctcc ttttccctcc gtatgcatcg ttgccatata tttgtattca
     3301 gagctgaacg gaatctttgc ttctattttt ttcaaaggca gattggctgt ttcagtgtgt
     3361 cgaatcagtg caagttctgt cggattgcca cttaaatttt ctgttttctg ttcttctttc
     3421 gtctttaact cctgacaatt tttcatatta gacagtacgc gagtttcttc ctttgctgct
     3481 acatccacaa ctgtcatttc attttttgtc aatgtccctg tcttatctga acagatcact
     3541 gtcatagaac ctaatgtttc tacagaaggc agtcctttga tgattgcctt ttcttttgcc
     3601 atttcattta ctccgacaga taggatcatg gtcaaaacag ctggcaatcc ttcgggtacc
     3661 attgacacca ccaaagcgat catagctgaa agaaggatag gccaatccat gccatagctg
     3721 aatgttgtaa aaacaactaa gaacaagatc aaacaaagaa tgccttgaaa aatttgtttg
     3781 ttcaatcggt tgatcttctt gatcagaggc gtcgtttgtt gttcgaccga ttgcagtgct
     3841 tgattgattt taccgatttc tgtcgtgttt cctgtttcta caacgacacc gactgcagaa
     3901 ccagattgta caagtgttcc tgaaaaagcc atatttttct ggtcaccgat tgattcttct
     3961 gctgccaaaa cagtatgatt tttctcagat gcttctgatt ctcccgtcaa aatcgattct
     4021 tccactagta gatcatgtac ttcgatcaac cgaacatctg ctggaacaac gtctccagca
     4081 cgtaagacaa tgacatctcc ttttactagt tctttcgcag gaattttttc ttgcatcccg
     4141 ttcgatagta ctatcgcttc ttgtcccatc agctgcttca aaccattcaa cgattcttct
     4201 gcttttcttt cttgccaata actaacgagt ccgttgacga tcactacgaa taaaataata
     4261 cttccttcga tgtattcgct cgaagcaaat ttcaaaaaag aggcaatgat caacacgatc
     4321 atcagcagat cagtaaagtg ctttactatt tttttccatc gttttgtctg ctgtcttgct
     4381 tctatctcat tatatccttg atcagtcagg cgtttctgcc gttcttcttg cgacaagcct
     4441 tctgttgatg tttgatagtt tttcaatgta tcttctaccg tcaatttata ccagttcatc
     4501 ctatcccctc ctacttaatt gatactttta ttatacgtag ggagatcttt caggcgtatc
     4561 gattatttca gagaagtctg aaataattcg ttttgccgga tatatttagt cttaattgat
     4621 atttctatcg cttattcttt tatatacaaa acttaagaga acaaaccaga taactaataa
     4681 aaaaggaata aaacgtgtca ttctttcgaa taacaagctg aaagcaatgg ccacaaaaaa
     4741 taccaacaaa gcaaggtctt tatattttgg tatagtgttc cgtttctttt ctttaacaaa
     4801 ttgaatatgt gcacataaga tcgaagacca cacaaataga aaacaactcg ttgtcatacc
     4861 tgccaaaagc tgaaaaaaca atcgactatc agcgacaaaa aaatgtagac aaagacctgc
     4921 aagaaataac gttcctgtaa tcaaaatacc gtgtaaaggt acgcctttct tagaaagcgc
     4981 acaaaaccaa gggggcagac ttttttcttt tgctgtttga tataagatcc tgctagtact
     5041 gtaaaccgca ctatttgccg aagacaaaga agaaaccaga atcatcagat tgacgattgc
     5101 tgccccgctt tggcaatgga tcatttgcaa catagtgact aaaggacttt cttttaaatc
     5161 taaacaatgc catggaacaa cgaataaaat cgacagaatc gttcctacat agaaaaagag
     5221 gatacgccaa ggcagacagt tgattgcatt tttcaaattc ttttgtgggt cactcgtctc
     5281 accaactgtt aatccgatca tttcaattcc agcaaaactg aataaaacca taggaaatga
     5341 agcaatcatt ccagcaaagc caaacgggag taattcattg agctgtccaa aatttgcttt
     5401 gattccgata gactgggtat gaaaatatga gacgctcaaa cctataccca ataaaataaa
     5461 aaaaacaatc cccaaaatct tgatgattga gaaaaaagat tccaattcgc caaaccattt
     5521 gacggcaaca agattgactg ccattaagat cattaatgca ctacctccgc ttatcaaagc
     5581 tggtacatgt acaaaccaga aatggatata caatcctatt gctgtcaatt ccgcaagtcc
     5641 agctccaagc cagcaaagcc aataagtcca attgacaaaa aaggcccact tttcacccaa
     5701 atagtagtca atgaaatcac tgatagagtt aaatttacta tttgctaaaa acaaatcacc
     5761 catagcccgc atcaagccat aaatagccac accgcatatc aaatagacga gaataacaac
     5821 tggtcctgcc agtgcaattg cctttgctga acctaagaat aatcctgttc caattgcgcc
     5881 acctagtgcg attaactgga tatttcgatt agttagagtc cgtttcattg ccggtttttc
     5941 catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc caagaaggtt
     6001 tctttatttt ttcaatacat agtggtacta caaacaaaat gacactgatg gctgtgactg
     6061 caattagatt gatccatgtt ccagctttca tcgtcagtcc gacagaaaag ccgattgccg
     6121 caaataaaac aataacgatc gcccagacgc caagattgct tttgttctct ttttttccaa
     6181 ttctgaatgg acggttcaac tttgcatgtg tataacgtaa tctcaaaacc gagatcccca
     6241 taatgacata gacgatacaa tagattacag tagttgaatt aactagcatc aaaaatgctt
     6301 gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg acagattgag
     6361 tcaaaataac ggatttggat acgtcaaatt gatttgtctt ccaaaaattg aattttggtg
     6421 gatacgcacc ttttctagca ctttctgtca ctgtctttgc aggtcctgat gcccaagcag
     6481 ataattggac ggccacaccg atgaagacaa gcaaactaaa cagattcaca ataagatgcg
     6541 gcaaaccgag aatttggcaa taaatcgaaa tagattgtgc gatattattc aattccattt
     6601 gaccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg aaaaatataa
     6661 aaatcagagc agcaaatact ccgcgaatat acgttttcac tgggttcttc aatcgagtga
     6721 tatacacgga ggacatttcg atgccagtaa aaataaacat gatcggcgca aaataaacaa
     6781 atgaggatgc agtcgtacta tctggtatta ctttgtccca tgaaaaattg cctaatgttc
     6841 cggttggtac gattccaact ttgatccacg cagcaagccc taataagagc atcatcgtca
     6901 aaggcacata tagacctaac caaacaccga tttttcctcc gatttttgcc atgtcgaatg
     6961 ttaaattcaa aaaagtgatg atccaataaa caactaaaat aacaactaat gtaaatttgc
     7021 tgttcagccc aagcggcaca ttatcgataa tattaccaaa caacggagcg agtgctgaag
     7081 caaccatcac catccctgga aacatttgga cccataacag ccaggagaca atgaatcccc
     7141 atttttttcc tagagcattc gatacccaaa gttctggacc acctttttga ggaaacattc
     7201 cggcaaattc accagagatc aatgagatcg gaaaggcata taacaatacg gcaaccagca
     7261 tgtaaaaaat catcgtccag ctcgttgctg ccacatctgg aatatttcgt acacttgcta
     7321 caagggcaca ggtcatcccg ataaaggtca tggtcgtgat ttgttttgat gaaccagcat
     7381 ctttcatctt tattccttct ttctttcaat agcgacatgt ttttcgtttt cttttatttt
     7441 tgttcaataa caaaagtgac aaggcttcat cgattcagta caaaacccag tatcaccgaa
     7501 acatccattg aacgaactta gtgaactggg tttgatacgt gaaacgaacg aaccaactat
     7561 agaaagattt ataaaaaaga atgtcttgct gaggaatata tgaaaaacag ggggaaatac
     7621 aactgttttg atgaggcaaa taattgtctg ttctccatat gtctcttgct attctttcac
     7681 acaaaaatca gttctatttg atgatttcgt ttagtttctt ttccattgct ttggcgattt
     7741 cttttgtata aaaattgaaa attcgatcat cattcaaata aggagtcatg atagccgcac
     7801 gtagcagagt gatttgctgt tctttttgcc attcttcttt cgatagaccc attctttcaa
     7861 taaaacggat cggagaatca ccgtaatctt cttgtgtaaa tgtcgtatga gaagtaataa
     7921 accgttcacc ataaacatcg ccatccatat aagaagaccg atcgaacatt ttttcattta
     7981 attcattgat agcgttcaaa tctgtacaat cttgtacttt aaatacccaa ttgaccatat
     8041 taaaatcagg atgattcagt ggatagactt cgatcgtttt tccttttaca gtaaagtgta
     8101 attgttctaa aaattctctg aatctttgag ctgcttcgat tgatgcacca atcaattgac
     8161 catatccggt cacattcaat ggcaatacac gatgagctgt ccagactgca gcagccgtcg
     8221 ctcctgcttt tgagccttct aaaatataag cacctagcat atctggtgct tcgactgatt
     8281 tttcaaacac atatggtgca aaataagaaa tgatatttct catattttta tgtttgatta
     8341 cgattcctcc agcagcataa gggacatagc ccatcttatg cggatcaatc gtgatagaat
     8401 ccgcttctga aatagctctg aatccttcat acacctcttt gtcgatcgtt acgtaatggt
     8461 ggaatacatg atgctcttca aagaattcag ctaaggaagc atatggcaca aattctcctg
     8521 cttcgtttaa aaacagtgaa cgcgcgtacc cgccatatgc agcgtcaaca tgtaaataga
     8581 aatagatacc ttcatctttt aacttttctc ttaactggat gattttatct acactgtcta
     8641 cttgtccttc ttctgttgtt cctacgacag caaccacacc tagaatcggg attttttgat
     8701 cagctaattc acgaattgtt ttttccaaag cgttgatatc cattcgataa tcctcttgaa
     8761 ctgggatagc aaccatctga tctaagccga ctccgcaaat atctaacgcc ttcatccaag
     8821 aatagtgttt tgtttgagga accagccatt tccctaattt ttggatattc tttcctctac
     8881 gagaagaagc ggctttgacc ttatccatct cttcatccgt aaagctttcg gtcatttcca
     8941 aaatttcttc aacagacaga ttcaaaagag cccactcaga catgtttttc actttttcag
     9001 gcagaatttc tttgacagct aaaggaatcg atttgatgca acgggcgtac cataatcctt
     9061 caaggttagc aatagaacca tctgctgtaa tatgtcccca tccgtctgcc atgttgaata
     9121 gtgaggcaaa atcctgtcct acttctgctt ccatctgcga agttgccata gaagattcta
     9181 gtgcaacgtt attgggattc catagcatcg cataattgta agctaataaa gcaggcatca
     9241 gtgtttcagc gttcatttgt ccccagtaac ggcctgctga atgccagggg atcgatcctg
     9301 cgcgtaatcg ctgactgact tcatccaaga cttctatcat atggtcgcgg gtggctagat
     9361 aatctggagt tagcttatct ccttcgctaa ttgcaggcat atctgaagga atataatttt
     9421 ctctccagcc aagatgttca tctaccatct tgttcaacaa cattttgtaa actggtccat
     9481 tttcagcttt gtcgcctata aagactgcct tgatatccat atctttcatg tttcttccct
     9541 ccatatttcg ttggtcaatg tctcgcagat acatgtgctt atctgtaaca cctcttcact
     9601 atagcgccaa aaatgagaaa atttttttta taacatcaga ttcacgtaga atatttcaaa
     9661 caaaaataaa taaagtcgga tttttctgat aattgaacgt ggaaaaacga acaatttttt
     9721 aataaatcag acttctaaaa tggatagtct aaaaaaaacg aacaaataac ttaaatgacc
     9781 atcattcatt catcaactgt atttgcattc cttttgcttg ttagaaattc acaatctatt
     9841 ttctaatgaa aatatttatt tttcaatttt tataacataa aaatctttca ttaacggaat
     9901 ttacaggatt tttcagaaat taattgatga tttccaaaag aaaacatgtc ataatatttt
     9961 ccatattgtg caaaaaaata caagatggag gctttactat gattgaagaa tatattgaga
    10021 agaatatttt gagacagctg tttttatgtg gacaattcta tgtaaataaa gaagtcaatc
    10081 tagaaaagct ctccaatctt ctacatgttt gcaaaaccac tttactcaat gatatcaata
    10141 acataaaaaa agaatttgaa gaacaaattg tttatacaca tcgggaaaag gattgttaca
    10201 ctttatattt ttcagaacat atacctcgat gtaagatcat gcagcaatta acccaaaatt
    10261 cgttgttttt aaaaacttgc ctgctttatt tggaagaaga tgaacctgat tatctccaat
    10321 taactgagtg tgaattcatc tctgtcagca aagcttactc cttaaaaaaa caagtattgg
    10381 cttattttca tgattgtggg atcgaaatcg accgctactc ccctcgattc actgaaatgg
    10441 aaagacgtct gttgcttctt aatgtggctt atcgtttagg cagctttaag tcctgggagc
    10501 tacctgaatc gttttttgaa cgtgcggatc gtttcattga atctgtaacg gagaatagcg
    10561 gtagatttta tgataaagaa aataaagaaa tcttatcgat tggttttgct attagtttct
    10621 tgcgccaaca gttgggtact gtgacaatag atccaacatt tatcgaagaa atcaaaaaac
    10681 gtcctattta caattatgtc gagtcaacat gggaaaccac tgatttccaa acctattata
    10741 aaaaagaaga atttgtcttt attttgacac tgttcaatct ttgtaattat ggtttccatt
    10801 cttatcagtt gattgcggaa gattttcaac agctacacca agtgtttatt gataatactc
    10861 ccgaaataaa agaactagtt gctacctttg aaactcattt ccgtcaagaa ttattcggga
    10921 atcagccatt tgaacgtgca ctgattcatc tgatgcgttc ggcttgggat aactatcaat
    10981 tattcatgcc ggaaaaattt tatcttctaa atgaggaaca ggcaaattta tacaaagacg
    11041 tccaaacact tttttccagt tgggccaaac ggctacctta tgatctacgg tttaatccta
    11101 attgcatgcg agcttttgtc atcgaattga gcggaattct ccgtctgaca aaagaacgtc
    11161 tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg
    11221 aagctgtaac tactttcgat tttcaagttg caccaacaat ctatgcaagt atatctgata
    11281 taaagaagta tgcacagcaa ccctcgaacc gagtgttatg tgaacgtaca ttatatacac
    11341 cggatgcagt ccaatatgag aatattattc ctatttcaat caatacgatc gaacgagcaa
    11401 tcatttcagc tgtgcaaaat aaataagagg ctgtgataaa catcatgtca cagcctctta
    11461 ttctgaataa acaatattcc aaaagtaact tcttcggaaa taagccgaaa ttttcataaa
    11521 tttcttctta ttccttgaat ctgcccactt cttaacaacc tctcgataaa cggcgggctc
    11581 atagcctcac ttatttggga cgtctactgg cccgatttct ttatttaaaa aataagaaat
    11641 gatcgttcgg ataactacca atgcagctag ctggaaaata tcctgcaaag taggtttgat
    11701 aatcgattcg atgatcccag ccgaaacaag aatttctaaa cttaggagta tataacctcc
    11761 taacatcttt ttaatagcat tgttctcgct gactcggtcg tttttcgctt ctgtatggac
    11821 aaataagtat ttcaagaaat ccttaatcga cagacaaacg ccccaaatca ataaacagat
    11881 agcaaaaata tcaagtccca gtacaagttt ttcaaaatat ggaagaagaa actgcatcaa
    11941 ctctgtcgtc atcctatctc tcctcttctt tgtttctctt tgtcttctat ttcctgattc
    12001 aagaaataag aaatgactgt tcgaatgata ataataagtg caagcttcaa aatatctgtc
    12061 caagtcggct tgataactga ctcgatgata tcagcaacga ctagcacttc caagcttaac
    12121 aaaatataac ttgctaacag ttttcttgta tctgcattac gagtattacg ttctgcatag
    12181 tttttcttga catgagacca atcaaaaagt ttgaatagaa caagaacgat cccagcaact
    12241 aatataccga tagaacaaat atttaaaata gcaatgatcc attctagaaa ttcaatcaaa
    12301 tttgagtgaa acatataagc tacctacttt cttttgattt gttcctattc ttttattgta
    12361 aaagaaagag accttgttcg tctatttaaa agttgtttta ttgatcacac cttgcctaaa
    12421 atcctcctct tccctttctc attcttcacc ctccatacca tccatacatc atggaagtat
    12481 ccaaaacaac atagcaagtt aaaataaggc ttagtccgtc atcagctttt caaagtggca
    12541 ctgtctcgcg ctttttttac ccaaatttct aaaaatagaa ccatacatct cctcaaaact
    12601 tttcttcttt cctaccctat tactaattac aggaaaatca gacacttctt cttttttgtc
    12661 aaaaattcct ttttcttctc accctatcat ttggcattct cataaaaacg aactatcctt
    12721 ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg
    12781 catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaaa cagggctatt
    12841 cgaagatgac agtcatttat atgatgaatt tgaatccatc tagcatctta tttcgttgaa
    12901 tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata
    12961 atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa
    13021 tggaaagggg accagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca
    13081 ccgaccgaca ctgtagtgga tgaatattgg aataaaaatc atccattaga aaaccaaaaa
    13141 ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat
    13201 ctattaagtc gaataccttc tgttactgag ccaggaatga ctgctgaaga agatacgaga
    13261 gattttgatg caaaacaccg tacctttgac aaagcacgtt tgatggaagg tggtaaaggg
    13321 atcattgacg gcggaaaact gggcttcaac aacaaggacc gtattcttct gactaaactg
    13381 attgccatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc
    13441 aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct
    13501 ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa
    13561 ttcactcaaa tcgaacattt agtgggcgtc aatcgaacac gttacaatca gtacgaaagt
    13621 atcatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc
    13681 cgtgtcactg actgggaatt caaagataca ccgatgcaag atgaaatcac tgtcactggt
    13741 ttgaaaatga ctaatacatt gactcaggaa gaggaacatg tcacggtaga tgaagataca
    13801 gcggtcattt tcacgaatgg ttccattaca gattcagcta ctttgggaga ttacgaaaca
    13861 ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagcgaa
    13921 cgtttctata acttaggaaa tcccgacaaa tttttcgcgg atcgagatgc gagcgaatgg
    13981 gtcagcttta cactgacgac gaaaaaccac ctgttattga atgaaatcac ccgtatcacg
    14041 actcaagaac caggaaatgc cttgaattca tttatttcaa ctacaccgat cacgccatta
    14101 ggccaaaaag atgtcaatat gtctatcgtc gtgcatcatc aaccgcattt tacgtctcaa
    14161 aaaccgaatg aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt
    14221 gtggataaac agtatattaa gatgaacgga aaagaaatgt tggaagaact gatcggacaa
    14281 ctttcaaaag tagatcctgg tccagtgaat attcgggaaa aagaaacaga gattttcgac
    14341 agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcattatt taataaccgt
    14401 gcaaaaagtg atcgaccaaa agtcattcct aagcattcaa ccaatctggc ctttacagga
    14461 gaatttgttg agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga
    14521 gaaatcgctg ctttccattt tgcgggcatc cctatgtccc gtcttgtcaa aacaccgcgt
    14581 tacgacaaag acatccctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga
    14641 ggttgtgaaa aagctgcctt gcatcaagta ataagaacag ccaagcaaaa acagctactt
    14701 atgtttttga cttggttgga cttattatcg cagatgcaag cttttgaaca ccgtttatca
    14761 agaggttgtg aaaaagacgt ttaatttcga gtattaagaa aaattttgtg aaaatggttc
    14821 ctcacatttt tgcataattt ggcttaatac cgagaaactg gcttttgaac accgtttacc
    14881 aagaggttgt gaacctaccg tttagctctg agtcacaaga agcaatttca aaaaatagct
    14941 tcctatgttt tatgaatatt gtgaccgagg agttggtagg ggaaccccat ttattcataa
    15001 caagaggttg tgacagattt catgtcacaa cctcttatta tttatatccg tttctttttg
    15061 aaaaaacctt tttttcagta ttcactctct tgccaatcca ccataatcca gctgctatca
    15121 ctacttcaac gattgctact acactaaaaa attggatgaa tgacaaaata tatgtgtcta
    15181 acaaatgttc gagtgcttgg gttcgaatat ctaactgact ggccatatcg tacaaataga
    15241 gactgactga cagacttaat aattggaagc ccgcacctcc tatcaccact gcaaatgacc
    15301 aaagaacttt tttccaagac attctgtata aataaaacac tccgctcagc aatacaagca
    15361 agatactact gatcattgga agaaaagaaa ccattcgctc tcgtatcgtc aatatctgat
    15421 cgatgaacga catcaaatag attgattgta ttcctcgtcg aaaaatccca gaagtcatat
    15481 agatgagtaa attgttttct tcactagcaa atggatcatc ctctgcttct tcttgtttgt
    15541 atgtattgat tatctcttgg agcttttctt ctacttcctt ttctccaatc agttctactg
    15601 gttctttttc atatctgttg cggatgaaaa gctcgatatt tttttgtact agttcttcag
    15661 aaacaatctt ttccaatgct tcagccggag ctccgatctc ataatgatag ctttggatct
    15721 ctcgattgat ctcagcagct acttttttcg catagtccga ttgcatgttt tgctctaaca
    15781 taaaatgttg atttgccaag gtgcttatta aacaaagcat aacagtcagg caaaacaggc
    15841 agacagaact aatcataaca atgatccgtt tattccattt attttgaatt tgtttcatta
    15901 cgcctcctcg tatttgtttt ttcactgaaa cgaatgatgg ttggttattg tttaaatagt
    15961 taaaatgatt caatagttca actatacttt actgtacgta aggatacaat gaaggcacac
    16021 tccttctttt tctaataaaa aaaaattttg gaactcttgc ttttttgagg atttttattt
    16081 tgagaaaaaa gcgtttcatt atcaaaaaga taagaactcc tttagaatga aactatctgt
    16141 agtaccaata atgcagacaa ttttgtgaat ctgaaaggag catctccaat gaaagcagtc
    16201 acttggcaag gaaaacaaaa aatggaaatc agaaacgtag atgatccaag aatattagaa
    16261 ccaacagacg caatcatccg tatcacagca accgccattt gcggctccga tctgcatctt
    16321 taccatcatg gtgaagcagt tttggaaaaa gggtatgttg taggtcacga acctatggga
    16381 atcgttgaag aagtaggatc acaagttcaa acattgaaaa aagggcaacg agtggttatc
    16441 ccttttaata tcagttgtgg tcattgtcat ttctgcctga atcacatgga aagccaatgt
    16501 gatgaaacaa atccagatca gttatatggc gggcttttcg gttttggcaa gttgaatggc
    16561 aatcattggg gtgggcaagc agaataccta agagtacctt atgcagactc tacgtcgttt
    16621 gtcgttccag ataataatct tcctgatgaa aaagttttgt ttttatcaga tattttgcct
    16681 acagcttatt ggagcgttga aaatgcaggc gtaaaaaaag gcgatacagt tgtgattcta
    16741 ggatctggtc cagtcggtct atttgctcaa aaattcgctg taatggctgg agcagagcgc
    16801 gtgattgcag tggatccagt acaacatcgt ttagataaag cggctaatta caacggcgtg
    16861 gaaacttatt tcttagaaga cacagcacaa gctggaacgg aattgtatga gttggctaaa
    16921 ggtggtgccg atgtcatcat cgattgtgta ggaatggatg ggttagaacc tgtaaaagaa
    16981 aaagcaaaaa atttggtgag cctacaaagc ggcacgattt ctccattaca gatggcttcg
    17041 caagctgtca aaaaattcgg aaccgtccaa gtaactggtg tgtatatgac gccagcttct
    17101 tcttacccgc ttcaagagtt tttcatgcga gatattgcgg tcaagcatgg acaggcacca
    17161 attatccatc tgatgccgaa aatctacgaa atgattgcag aaggactatt tgatcctaca
    17221 cagatcatta ctcattcgct gccactagaa gaagcagcac aagcatatga gatctttgac
    17281 aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt gaaatcagta aaaaatacag
    17341 gtgattttct ttatgcccaa aatcaaacga aatacccatg agatcgaaat aggaaaagaa
    17401 gaagatatcg aaatacgagg acgtcgcttc cgcttctatt tccaaaaccg ttacacattg
    17461 atctcactag ctgtcgactt attaaccggg attttctata tttttggaag ccttgcttcc
    17521 ttaactaaaa tccccgatcg atatggcacc tatttttatt tggctggtgc tgttttcttg
    17581 acgattcgtc ctattctcag aatcgttagg aacattttca tttacgacga gaaaagaaca
    17641 aggaaaaagc atgggaaggc agtttagcct tctccatgct tttttgttat tcttgtgttg
    17701 tgcatatttc gcatactgcg attcgaaaaa tttctaagta tagaacaatt tttcggtttc
    17761 cttctctata tactcacgtc tatgctgttt catgtatcta ctcttacagc gtcatttggt
    17821 acactcgcac aggaattcct tcattatcct gagccatccc gataaactgc caattgtatt
    17881 tttcataaaa atccgtatga tccgtcacaa ggtaaagcac ctttacgccc atttctgcaa
    17941 agtcttgctt gatcgtttct aaaatcattt tggcaatccg ttggtttcgg tagtcttttt
    18001 caacaaacag cgcacagaca ttcggagtca aatctactcg atcatgaaaa tcattgtcaa
    18061 caacacctgc acctgcaata atctctcccc attcattcaa caccacatac cattgcggaa
    18121 ccgatgtgtt ttttctcgtg cttgcttgga tactttcttc ataaacagca actggcagat
    18181 gccattttga agcaaaccat tcggctgttt cttttatcaa tgtcggtcga tcccgcaatt
    18241 ttatgactgt tcttctctcc atttgtactc tctcccattc atatttggtc tattatagca
    18301 ccttgtattt caaatagctt tccaatcgct cgattttttt gaatgaatgg gattttttat
    18361 ttatacacaa atttcaagac aaatatacct cgattttctc attcattatt cgatcctaag
    18421 ttttaaatta cttatttcca taaaaaaaca gaaccagata accggttctg ctttcttttt
    18481 tcattaagca tccaaagctt cttttcttct ttcttggatc gtagctacct tgacgaacgg
    18541 cagatagatc aaaatagaaa ggatcagatt gacagcggcc agtacaccac ctgcaatact
    18601 cttcgttgcc aatacaccgc caataactgg tggtgttacc catggtggca taacagtagc
    18661 tgctggcact aaacctgtag aagtcgcaaa gtacgcaaca ctaactaaga ccattggtgt
    18721 caaaataaat ggaatgaaca tgattggatt caatacgatc ggtaaaccaa acatcgttgg
    18781 ttcattgata ttgaaaacac ctggcgcaat cgacaagttt gttacaacca tatacggttt
    18841 gtttttacgt cctactaaat aaatagctaa cagtaaacct aatgttgca
//
LOCUS       DACCQI010000061        18840 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCQI010000061 DACCQI010000000
VERSION     DACCQI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.062"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(21..251)
                     /locus_tag="IVO76_001665"
     CDS             complement(21..251)
                     /locus_tag="IVO76_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429628.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IVO76_001666"
     CDS             complement(687..1253)
                     /locus_tag="IVO76_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7429629.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IVO76_001667"
     CDS             complement(1253..2209)
                     /locus_tag="IVO76_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7429630.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IVO76_001668"
     CDS             complement(2209..2865)
                     /locus_tag="IVO76_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7429631.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IVO76_001669"
     CDS             3235..5253
                     /locus_tag="IVO76_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7429632.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IVO76_001670"
     CDS             5680..8094
                     /locus_tag="IVO76_001670"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7429633.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IVO76_001671"
     CDS             8442..9995
                     /locus_tag="IVO76_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7429634.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IVO76_001672"
     CDS             complement(10376..12160)
                     /locus_tag="IVO76_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429635.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IVO76_001673"
     CDS             complement(12175..13896)
                     /locus_tag="IVO76_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429636.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IVO76_001674"
     CDS             13996..14757
                     /locus_tag="IVO76_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7429637.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IVO76_001675"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IVO76_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18840
                     /locus_tag="IVO76_001676"
     CDS             16664..>18840
                     /locus_tag="IVO76_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429638.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         6186 a         3343 c         3771 g         5540 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
//
LOCUS       DACCQI010000062        18634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCQI010000062 DACCQI010000000
VERSION     DACCQI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.063"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            114..569
                     /locus_tag="IVO76_001677"
     CDS             114..569
                     /locus_tag="IVO76_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7429639.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            1039..1308
                     /locus_tag="IVO76_001678"
     CDS             1039..1308
                     /locus_tag="IVO76_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429640.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            1708..1962
                     /locus_tag="IVO76_001679"
     CDS             1708..1962
                     /locus_tag="IVO76_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7429641.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(2051..3097)
                     /gene="holA"
                     /locus_tag="IVO76_001680"
     CDS             complement(2051..3097)
                     /gene="holA"
                     /locus_tag="IVO76_001680"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7429642.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(3135..5408)
                     /locus_tag="IVO76_001681"
     CDS             complement(3135..5408)
                     /locus_tag="IVO76_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7429643.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(5401..5940)
                     /locus_tag="IVO76_001682"
     CDS             complement(5401..5940)
                     /locus_tag="IVO76_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7429644.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(6076..6294)
                     /locus_tag="IVO76_001683"
     CDS             complement(6076..6294)
                     /locus_tag="IVO76_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7429645.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(6291..6536)
                     /locus_tag="IVO76_001684"
     CDS             complement(6291..6536)
                     /locus_tag="IVO76_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7429646.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(6652..7380)
                     /locus_tag="IVO76_001685"
     CDS             complement(6652..7380)
                     /locus_tag="IVO76_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7429647.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(7539..8582)
                     /locus_tag="IVO76_001686"
     CDS             complement(7539..8582)
                     /locus_tag="IVO76_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7429648.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(8572..9063)
                     /gene="coaD"
                     /locus_tag="IVO76_001687"
     CDS             complement(8572..9063)
                     /gene="coaD"
                     /locus_tag="IVO76_001687"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7429649.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(9060..9620)
                     /gene="rsmD"
                     /locus_tag="IVO76_001688"
     CDS             complement(9060..9620)
                     /gene="rsmD"
                     /locus_tag="IVO76_001688"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7429650.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(9739..10077)
                     /locus_tag="IVO76_001689"
     CDS             complement(9739..10077)
                     /locus_tag="IVO76_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7429651.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(10061..10498)
                     /locus_tag="IVO76_001690"
     CDS             complement(10061..10498)
                     /locus_tag="IVO76_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7429652.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(10488..11612)
                     /locus_tag="IVO76_001691"
     CDS             complement(10488..11612)
                     /locus_tag="IVO76_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429653.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(11633..15061)
                     /locus_tag="IVO76_001692"
     CDS             complement(11633..15061)
                     /locus_tag="IVO76_001692"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7429654.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(15087..16250)
                     /locus_tag="IVO76_001693"
     CDS             complement(15087..16250)
                     /locus_tag="IVO76_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7429655.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(16253..16555)
                     /locus_tag="IVO76_001694"
     CDS             complement(16253..16555)
                     /locus_tag="IVO76_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7429656.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(16669..18441)
                     /gene="recQ"
                     /locus_tag="IVO76_001695"
     CDS             complement(16669..18441)
                     /gene="recQ"
                     /locus_tag="IVO76_001695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7429657.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         5336 a         3918 c         3171 g         6209 t
ORIGIN      
        1 tttattttat gaaaaaaaca gcttatgaat aggtcttgaa ggatgaattc ctacttttgt
       61 aactttctaa aaacatgtta tgattgtttc gaagaaagtg aggaatatat ttcatggaaa
      121 gttggctttt tttaggaatt attttacttg tagcaatttt agctaaaaat caaagtctga
      181 tgatagcggc agcttttgtt ttgattatga agctcatacc agcctcccgt tcctttttcc
      241 cttggcttca aggcaaagga atcaatattg gggtgacgat catcacagcg gctattctaa
      301 tccctatcgc aacaggagaa atcggtctac gtgatctatt agaagccttt aaatccccta
      361 ttggctgggt agctgttttt tgcggtgggc tagtggctgt cttatcttcc aagggtgtag
      421 gattgattgc ttctgatcca caaatcactg ttgcacttgt tttcggcacg attttaggtg
      481 ttgtcttctt aagaggaatc gccgccggac cggtcattgc ttctggtatc acttactgta
      541 tcttacaagt atttgcttta ggaaaataga acaaattttg tctattttag gagattctta
      601 acacccccaa tttactgatc cgtgatatac tgacaacgag gtgatacaat gttcgaaaaa
      661 ttaaagtatt tcataggaac aatcggctgg tttcttctag cggtactttt gattctgggc
      721 ttagcttatc tattatttcc tgatttgata caaaatgtgt ttgatactgc tttttgattg
      781 acctattaaa acgatatagg ggatgctcct gcgatgcagg ggcttttttt aatcaatgtt
      841 ttgtaataat ttaggccgtg ccaatgagac ttcctataga attcgatgct cttttttctt
      901 tttggctctt ctgctctttt ttgttatact agattacagt ggacaaaaga aagaatgatc
      961 gcatccactt caacgaatat caaaatggaa gaattagtgc ctgttttcag gttactttga
     1021 aaagaaagga agacgcttgt ggaaactttt gaaggtcttt taattgatac gtacgtttta
     1081 caagaacagc caatgatgct gactgggatc aacttgcagc ttgaggaccg tgtgatcaat
     1141 ggcctgctta aagatcgcta tttggcaaaa tatatcttac gtttgcccct aaataaatat
     1201 gtgctggaag taagtggcaa atggaaccgt cggaaccagt tggtcataaa aaaaatgttg
     1261 ataaaaaata tcgatgaata cattaaaata atcggtacac cagaatagcc tttactgatc
     1321 aaaggatact ggttataaaa taactgcagt ttcttaatag aaaaacaaag aacaacaggg
     1381 aatcgtatcc cttttcaaaa aaaactttgc aaacggttct ctttgttata taataactta
     1441 gtaccaaaca ttcccattca aactttgttt aaatagataa ataaaaatct agtttggttt
     1501 gacgctattg cctcccacaa tagcgtcttt ttgatttttt tcagcttgta ctatccgttt
     1561 ttactgaaaa aaaatcctaa taaaagactg ttaaggaaaa aaacttgtca tgttcatatt
     1621 gcaaagcacg ctgcctttag gtataataat gaatgttgaa ttatgcggta acacgcgtaa
     1681 gaacttaagt tatcggaggt gaaactaatg ccaaacattg aatctgcaat caaacgtgtt
     1741 cgtacaagcg aaaacgctaa tgtgaaaaat tcatctcaaa caagcgctat gcgtactgca
     1801 atcaaaaaat ttgaagatgc tgtagcttca ggtgctgaca acgtagatgc gttatataaa
     1861 gaagctgtta aagctattga tatggctgaa tcaaaaggtc taatccacaa aaacaaagct
     1921 aaccgcgaca aatctcgttt aagcaaaaaa atcgctaaat aaaagaatcg gcttcggccg
     1981 atttttttat ttgtcaaaaa aaggacaaaa acaaacgacc ttcccgcatg ctttgccctc
     2041 gtctttcttc ttatcgattt cttaacaact gccctgtgcg caaaacgaat agttgaaaac
     2101 ttaattcttt ttcgatttgt cccgttttga tttgataatc catctcaact aattgatcaa
     2161 acaactgtac cagcaaatgt tcatcgaatt tttttacttc ctgcatggca agttttacac
     2221 gatatggatg gattttcaat gtttcagcaa tatttgcctg ctgataacca atctttacaa
     2281 gaaattttgt ctgtaaaaat aaacgtaatt gagaaagcaa aatcgcatta attttgattg
     2341 tttcttcccc ttgcataact aaatcttcgt ataaacgcaa agcttgttcc gtcttccctt
     2401 ttaaaatata ttgggtcatg tcaaaaatat tatgttctaa cgttttagga actaattctt
     2461 ctacttctct taccgtgatt tgctgttgat tttgtccata aaggaccaat ttctgtaact
     2521 ctctcatcat tttggataaa tctaagtcgg tcaacctcaa gaataaatca attgcacttc
     2581 gttccatttt gattgaggaa ttctctaagg tattcatcag atattgacgt acttcttttt
     2641 cattcaactg ttggacattg atgataattg ctgttttttt caattgtttc gttacttttt
     2701 tccgttcatc caacttttca tatggcgcaa agaatactaa tacagtcgat tctaatggtt
     2761 ctttcaaata agccgtcaat aaatcagtat tttgttcgat tccattgttt actttttcac
     2821 ctgttaaaaa ataaggattc tctacgaata ctaaccgttg atctccgaaa aacggcaacg
     2881 tctctgcttc agctactacc tcatctaaac ttgcattttc catgtcaaaa cttaaaaaat
     2941 ttaagtcatc ttcgccctct aattggattt ttttgacaat ttctgtccga acttgatctt
     3001 ggagaaactt ttctgttcct aaaatcaagt aacaagatgc aagtttttct ttacgtattt
     3061 tttgtaactc tgcttgtata ctcaaattac tcaccacttt caaaaattat cctaccatga
     3121 gcagatgaga attactagtc tatcattgtt tccactagcc catgaggatg aaaaactgga
     3181 tgccagcgat aggtgatcat tccttgttga tccgttcgcc atgtttttat ctgattttct
     3241 tctagtgtct ccaatacttc gctgtgcggg tgaccataac gattatttac gccgcaggat
     3301 ataatcccat gttttgcttc aatatgggaa agaaaatctg tgttacttga cgtacggcta
     3361 ccgtgatgac ctaatttgac tacatcggct ttcagattag gataaatcaa aagcaattgt
     3421 ttttccattg cttgattcaa atctcctaaa aataaaaagc gcctattgtt atattccatc
     3481 catagaccaa tcgaatcttc atttcttccc tctccctttt caaaaggagt caatacataa
     3541 agatcataat aaccgctgat cctgtcccca tgtttgattt caatgatctc agtaccattt
     3601 ttttgagctt cagataaatg cctttgtaat ggctcatttc gtccagcgcc ccaaccgata
     3661 taaatttttt tgatagtaaa atgctgagat aacgtgggta attctcccac atgatccgta
     3721 tcgtcgtgtg tcaagatgag tttatcgatt acacttatcc cctgaccttt tagatatgga
     3781 acgatatttc tttcagcagg aggacgtatc ataccttgcg cccacttttc tctttcaaag
     3841 tgtaactgac ctccagtatc gatcaaaatc gtttcttttc gaaaaggact ttgcaaaaag
     3901 atactgtctc cttgaccgac atcgataaaa gcgatcgttg aagaaaagcg taaaaatggc
     3961 aaagtcaaaa aaataaaaga aaaaagacca acataaaaca agtgtttcac aggttgttct
     4021 ttcaacctat ccatacaaat cagtaatccg aaaatataca tcccagatag cacaaggggc
     4081 aaacgtccta taatgagaga cgaccctgaa gaaaaatcga ttattcgctc aaataagatt
     4141 aatccctctt ctatcagcaa aagtaaagtt tccggcagcc agcgattgaa gagtgtgaga
     4201 aataacacac caggaagaaa aagaaacttg aataccggtg caatcagtaa tgtaaaaaga
     4261 ctacctaaaa taggccactc ataaaaatgc catacaatca acggcatcgt gatcatagta
     4321 aacaggacgt gagaggccgt ttttttcaac caatctccat caaaagaaat caggataaac
     4381 aaaaaactca tgaaaaaact aagctgtcca cccgcatgaa aaagaaatcg aggatcaaat
     4441 agtaaaagaa gccaaaagac aactgcaaat aagtccatgg tggaataatg cttattcagc
     4501 aatttgacta gcaatcgcaa taaaaacagc aaactagcac gcaaaacaga aatagaactt
     4561 cctgcaatag aaacataaca gatagttagt agactcaacg gaataatact gacgtgtagt
     4621 ggccatccga tacgtcgaaa taaataatac agccacccta aataaaattg gatgtgtaga
     4681 cccgatagac tgaacagatg cagcaaacca ctctccctaa aaatttcttc tggttctctg
     4741 aatcctgcat ctttatagcc ccataataaa gaattaatat atacacttgt tttttgcgga
     4801 aattcagatt ggacaaagga gataaaagat cctctttttc tctgaagcca gtttcgccag
     4861 tgattttgca tggtgatctg ttcaatcttt tgaatttgga accgtccaga aattctatgg
     4921 ttcctgaagt aagcttcttg gtcaaaatta taaagattcc gcttttttgc tggagagatg
     4981 aactcgcctt cagcagtcaa ttctattccc tttcctcgga agttaagcca tgttttcaat
     5041 tctgcttctg tgtttacttg ataagaaagc tggagctttt gtttattctc tgataacctt
     5101 ccttccattg tcaaaaaatt accatttgca cgaatcgtat ctgtaaatac agtaatttgc
     5161 gcagatgttg ttttttcctc taaaaatgaa tactcttgta gcctataaga aatggatacc
     5221 gttatgctac acaaaataca caagtacgcg ataaaacgtc cttcttttag cgaaaaccat
     5281 aaaaaaattc cggcaagaag caaacgataa ccccaattgt cagtgtaata caacaaacag
     5341 ataatcatcg acgtaattgc cggaaaaatc aaccgatttc gaacatgcct ttctaaaacg
     5401 ctagacatgt tttcctgtta attcattttg aagttttgaa aaatgctgtg agtctaaagc
     5461 aacttggtgt acagatacac ctaattgttc gatcaacgct tgggcataag gatcatttcg
     5521 atagtcatgg agataattga tccctttcac accagcttgc aacaaagcct ttgtacaggc
     5581 caaacatgga aaatgtgtga catagatttc tgaaccttcc gtcgggatac caagttttgc
     5641 acactgtagg agtgcattca tctctgcatg gatcgttcgc aagcagtgtc catccacgac
     5701 gtaacaccct tcatctatgc agtggacatc tccactgact gctccattat agccgccagc
     5761 aatgatcctt ttttctcgaa cgagtgtcgc tcctacttct aaacgtgtac acgtactacg
     5821 taaagataac agtactgcct gagccataaa gtactggtcc caaggaatgc gttcattatg
     5881 tgacttatca gtaacttttt ctttattcgt atcgcatgct tttgaagatt tttctaacat
     5941 gttttatatt cctccgtttt ttcaagttga ttaaaaagta ctcattgctt aaacaaaata
     6001 ttctttaaat ataacgaatc atcctgtctg acaaaagatc ctacccttca ctgccgaaat
     6061 gaagtccgtc atccattagc ccattataaa aattcttcga atccatttgt tcaagaaatt
     6121 ccgtataaaa atcgatactt cacaatcatg gcgttttgct acttctgtga gaacagagat
     6181 atattctttg atcaacttgt tattccgata tttttgtttt gtctcatcga ctgctggtgg
     6241 cgtgataaag aaagcatgat cagaactata gtcttttttc agctgaacta tcatccactg
     6301 cagatttttt ggtacgtttc tttagaaagt tgtttatgct ctgctagatc attcgtccca
     6361 atgagaataa atactttatc tgctgtatag ctaagtacat ctttggcaaa gcgttctcgt
     6421 agagcttgac tgttattgcc gctgacagcc gtattcataa tatgaatccc agatatttta
     6481 ttttggagat agacattcag cattggtttt tttaatgttt ctttgcgaac aatcaagcta
     6541 tttcctatcc aaaaatacta cctcctcgtc ctgccaagct cttagaattg gattattctt
     6601 agtatccatt agcgtccatt tattgctcgt gccagtcgat tttgaatcat tctatttaat
     6661 tgtaacgaag ttttttagtt tttcaactgt cttttcaccg ataccagaaa tctcctgcag
     6721 gtcttccact ttctggaagg atccgttttc ttctctaaat cgaatgattt cttgcgcttt
     6781 gactgatccg atcccaggaa tctgctgaag ttctgatgga gtggctgtat tgatatttat
     6841 tttgggaggc tcggaatctg ctgtaggctg ttttccttcc gaggatgcca actgatcaag
     6901 atgttctggc agctcttcac ctacagcagg gatatatagg acttgctgat cttctatctt
     6961 cgcagaaaag ttgattcgat cgctatccgc ttcttctaat aatccacctg cacgctttag
     7021 tacatcatag acccgttctt ctgaagaaaa ggaatagata ccaggctcct tcacacttcc
     7081 tttgatatcg gtgtacatga cctttggacg agcatcctct tctacatcgt ttccttcagc
     7141 tgatggatct tctccactgg aactaaacga agaaagtatt tcttttgcag cagtctcttc
     7201 tgtttctcta taaaatagca aatttgataa taaaaaacca cttgcgatac aaaagataag
     7261 aactatcata gacatccaaa tgatcaattt gggtgaagct agtattttct tgctgatttt
     7321 ttctatcatt gttctttttt caaagaatga ccagtaaaaa tcattcatta ctcctttcat
     7381 ttaaaattga agaaacagtg agctctggca aaatcgtccc ttcaacatct aattacgtca
     7441 attttttctt ttgtttttat ttttatcgtg aagatttttg acgaaaaaga ggggttgtga
     7501 cagaactcgt gtcacaaccc ctctttaatt atttagtttc atttcatttt ctctagatag
     7561 tcaagcgctt cttgtactgt ttttactggt acgattttca ttgaggaatt caatttttta
     7621 gcggcagctt ttgcttcttc gtagttggtt tttatttcag gatattcttt cttaacatcg
     7681 tctgaaatcg tatcatctgg tgcaaagaaa atctctacgc cagctttatc cgcacttgcc
     7741 acttttttat cgattcctcc gattcggcca acttctcctt cactgttgat cgttccagtt
     7801 cctgcaattt gcttaccatg tcgcaagttt ttaccagtga cttgttcata gatttctaaa
     7861 gtgaacatca atcccgctga tggcccaccg atagagccag aattaatacg aacatctgta
     7921 tctgattcga tctcggtatg gtcagtaagc gtgataccta ttcctgcctt tttatctgtt
     7981 ggtaactcga tcagatcacc agaagctgtt ttttcttcac cattatgcaa ataactgatt
     8041 tcgattgttt gtccaacttt ttggcttttt acataatcca taaatgcttc tgtactttga
     8101 aagcttttcc cgtctacttt tgttactgta tctccaactg aaagcttgcc aaagaaattc
     8161 gatgttttct ctacactcat gacatatacg cctttgaatt ccattttata aggcttgtct
     8221 gcaagtttta acgcttgttc gattgctgca ttttgggaag aatccatata atactgttgc
     8281 atttgattat attcttcatt tgtcgcccca ccagtcaatt cttcttcact gatcaattct
     8341 tcaaaagggg tcattttagc ttttaaagct aagaaacctg tagcttgcct gataccaaca
     8401 gaggtaagag agaaagaccc agaaccttta tcttccttat cattgaccgt aattagttct
     8461 ttcagatcga tcgtggctcc cggtgattct acataatacg gcaagggaac tactacaaat
     8521 ccaatgatca acattccaaa tatgataatc agtactgttt tcaaccattg tttagctttc
     8581 atttgattca cgctttccag ctaaagcttc gttgatcact ggcggtaagt agctgctcac
     8641 atctccgcca aagtgcagta cttcttttaa aatactggaa ctcacgtgac tatattctgg
     8701 ttttgctaat aagaagacag tttccaattc atcatataga tgatgattca tctgagcaat
     8761 ctctcgttca tattcaaagt ctttgatact tcgtatgcct ctgatcaacg catctgcacc
     8821 aatctctttt gctgtttcta ccgtcaattg attttcttga tgcatgactt ttacattagg
     8881 aagatgagaa acagcttttg tgatcaatgt cattctttct tctgggggaa acaaactttt
     8941 ctttgaagta ttgataaaaa caccaattac tacctcatcg aataatttag ccgcacgttc
     9001 caccgtatct aagtgacctt ttgtaaaagg atcaaaactt cctgggaata atgcacgttt
     9061 cattgatttc cctcttttct atagatagtt acggccgtta tgccataaat ctgttttctt
     9121 atttgatgga atccagccac agtttctggc aattcgactg ttttatccgt ttcgcatacg
     9181 ataactgcgg ttttagaaaa aagtcccagt tcttccattt tatggatttg atcgattatt
     9241 ttttgttttg catatggcgg atcaagaaac aagtagtcga attgttgttt ctccgctgct
     9301 agccgttcga ttgctttgtc cgcatcaagt tttattgtgg taaatttctc tggttctttc
     9361 gtaatttcga tgttttcttt gatgattttt agcgcttggt aatttttgtc tacgcatacc
     9421 gcatgactgc agcctctaga tactgcttcg atagctaaac caccgcttcc tgcaaacaga
     9481 tccaatactg tctctccatc aaaatatggt ccaatcatat tgaatatgga ttcttttact
     9541 ttatctgtag ttggtctggt gttcgcccct gctaaacttt ttaaacggcg cccgccataa
     9601 tttcctgata ttatccgcat ctactcactc cttagaaatg gtactggtac tagtatacca
     9661 aaaaaatact tggacaatga tcaaaaatcg atcgttatcc aagttatttt ttatttaaaa
     9721 actgctcata agagattttt aaagatgttc agctaatcgg atttctgtat ttagctcttc
     9781 aatttcgaaa ccttcgtctt ttaatactgt tttgtttcct attttttcgg caaaattcat
     9841 ttcgatatcc ggtcggtaag aacgttctac gtttcgaaca aaatgtaatt tacggattcg
     9901 ttcttctgtt tcttccaatg tctcttcatt taagtaaagc acgacatatt tcattcttct
     9961 tgagacataa tgcaacaagc cgaacttttt cagattcttt aattgtttta agctgtagac
    10021 ccatacgatc agcccgcgtc gttttgttat ttcaaattca ctattgtcta attgcatgac
    10081 aattacctcc gcatccaaag tgttttttcg tttcaaaaaa cgggctacct gcatctactt
    10141 tgatttcatt agaaacttct tcggcaagta cttggacaat ttgatccaac agactttgca
    10201 gctgtgtttc actatatcgg aaagctgcaa catgctcatc catatcaagc tgtctttttg
    10261 cttttcgaac agctcgtttt ttttcacgat aatcaggtgc atactcacca tatgccgcta
    10321 cattttcaaa tgcttctttc ttagacaaga acgcttcttt caactggcaa acttttgcag
    10381 attcttccat tgactgttta tgttcaagat aagaagccat cgttttactc ttttttatca
    10441 tttcagcgac ttggaggcat tgatcctcca gttgaaacag ctcttcgtta acgatcactg
    10501 ctatcactac tttctccgtt ttcttcttgc aataagacta acatattttc tttagaaaaa
    10561 gcaatgccta cagaatccgt tcccgggtcc atgaaacggt catggttgta aggatcgctg
    10621 taccaatata aaaacgaaaa agtagcgtca atcgccggat gaataaatag cccactagta
    10681 ttttctaatt tttgtttact gattaacttc tccaattcat cattcgtcag tgagaatttc
    10741 actgtcgttt gagcactgcc tagcgatttt tgccaatcag ccatacgttc ctctgacaat
    10801 atttcctcag gccttgtcaa aaaatcatgt agtagcagct gactgtggtt ttgaaaggca
    10861 agtgtttgat tgtatatggg aagttcgtct tctcttctaa gcatatttaa taaagtcgta
    10921 ctttttcgtt ctttaacgcg attgattgat tcccaatcag ccgatttatc tgcttgataa
    10981 tgtggaaggc cttcaccaaa cacttgatat ggcaatagtt tcaaaagact atctttatct
    11041 aaatacacaa ctgaaaatag gccgtttgtt gtttgatcaa aaaataaaat agcaaaagtg
    11101 tcgttatcaa aagcaattag cggacgataa ttcatatcct cttccattag ttcgattcca
    11161 acttctgtat catggtactc aaaagtgaaa ttagtataga tagtagtgat tttcgtaaga
    11221 tcttgcatcg tcatccctaa ataaaacggc gcgatatcag caggccctgt ttgcagtact
    11281 ttaacagttg tgactctgct gtcttggatg ttcgcttcaa atgtgagatg actagcttta
    11341 ttttcataat atcttgtttc aaaaccaaat ccgctagtta atcgatcata ggcttggcca
    11401 aaatcctttt ccagctgatc gatcggtttt ccgatataag tagaaaaacc agttgcttta
    11461 agctcctggt atttccacgt tgtcatttgg tttgatattt gttgatcaga gttttccttc
    11521 acttcaagtt ttggcggaaa caagactggt tccatgtaga atgccacaat aactaatagc
    11581 agaatcagcc aaaacaaacc cagtcttttc atgtttcccc acctttcgct tattatttct
    11641 cagtcacttc aatcaaaagg tcaccagacg aaatagtgtc tcctgaagac acatatacat
    11701 gtgcgacttc tccgtcaaaa cgtgcttcga tggccgtttc cattttcatc gcttcagtaa
    11761 tcatcaacgt atcacctttt ttaacgttat cgcctttttt cactaataca tccaaaacag
    11821 aaccagacat cgttgctcca atttgttctc ggtttgtcgg ttcagctttc aatcgggttt
    11881 ggaccgtact gatgatcgaa ttatctttga tagtgatttc acgtctttgt ccattcaaat
    11941 tgaagaacag tacgcgattc ccttcgatat cgggttctcc gatttcatct aaacgaatga
    12001 taagcgtttt tccgcgttcg atttgtacat tgattgtttc acccaaacgt ataccttgga
    12061 aaaatgttgg tgtgtccagt aatgtaacat cgccaaattg attatatgct ttttgatagt
    12121 ccaaaaagac ttgcggatac atcaaatagc ttaatacttc gtcttgtttt ggttcatagc
    12181 caattttttc tgccaattct ttttttactt cgttgaagtc gactgattca gccaaaagac
    12241 ctggacgttc attcaatgca ggtctgcctt taagaataat cttttgcaat tttttaggaa
    12301 atccgccaac gggttgccct agttctcctt ggaagaaagt cacaacagat tcagggaaac
    12361 ttagtgtttc gcctttttca taaatatctt cttctgtaag atcattttgg accataaata
    12421 gtgccatatc tccaactact ttagatgatg gagttacttt aacgatatcg ccaaacatca
    12481 agttgacagt atgatacatt tgtttgattt catcccagcg atgacctaat ccgacagctt
    12541 ttgcctgttg ctgtagattc gaatactgtc ctcctggcat ttcatgcata tatacttctg
    12601 tttcaggagc attcaagccg ttttcaaacg gtttgtaata catgcgcaca tcttcccaat
    12661 aatggtttaa tttctgtgca ttttcaatcg tgatttctgg cagccgtgga ccattgacca
    12721 gtgcataata caagctgctc atgctaggct gactagtatt cccactcatt gcactcattg
    12781 ccacgtcaac gatgtctact cctgcttttg aagcagcaga ataagtgatg attccattcc
    12841 cgctggtatc atgagtatgc agatggattg gcagatctgt cgtttctttc aattcactaa
    12901 tcaaacgata agctgcctgc ggtttaagca gcccagccat atctttgatt gcaatgatat
    12961 gcgcacccag ttgttctagt tcctttgcca tgtctttata atactgaaca ttgtattttg
    13021 cccgacttgg atcgttgata tcgccagtat agcaaatagc tgcctctgcg attttaccag
    13081 tatcccgtac agcttggata cttttttcca tttgtggagt ccagttcaaa ctatcaaaga
    13141 ttcgaaatac atcgattcct tgcgctgctg cttctttgac aaattccacc aatacattgt
    13201 caggataatt agaataccca acggcatttg aacctctgaa aagcatttgc agcaacgtat
    13261 taggcattaa agaacgaatt tttctcaacc gttgccaagg atcttcattc aaaaagcggt
    13321 aagccacatc aaatgttgct cccccccaca tctcacttga aaagagttca ggaagacctt
    13381 ctccagtcaa acgtgcaatc tgcttgaaat cctttgtccg aacacgagtt gctagcagac
    13441 tttgatgggc gtctctaaat gtcgtatccg tcaacaacaa attttcctgt cctttgatcc
    13501 attcgaccaa agcatcggca cccttcgcat ctaatacatt cttagccgtt acgtaatcag
    13561 ttcttgtgat tagatcttta ggcatacgtg gttcttcata aaatggtttt tcgccacttt
    13621 cgatacctgg aaaaccattc acagtaattt caccgatata tttcatcgtt ttgtttccgc
    13681 ggtctcgtag tcgaggaaat tcaaataatg ttgctgtact atcaatgaag gttgtttttg
    13741 catttcctga ttggaattca ggatgtgtga tgacattgag cataaatgga atgtttgttt
    13801 tcactccacg gattctaaat tctttcaagc aacgttccat tttctgaatg gctgtttcaa
    13861 aggtagcacc atgcgtacat acttttacta gcaatgaatc aaaatacggt gtcactacat
    13921 agcccgcgta agcatttccg acatccagac gtacgccaaa accaccaggt gaacgatacg
    13981 tatcgatttt ccctgtgtct ggtaaaaagt tattcaacgg gtcttctgta gtgattcggc
    14041 attgaatggc tgaaccattt aatttaattc cttcttgctg aggcaatccg atttctttat
    14101 gcagatccaa accttgagca atcaacagtt gcgttgttac gatatcaata tctgtaatca
    14161 tttctgtgat cgtatgttcc acttggacgc gagggttgac ttcgataaag taaaaatcat
    14221 ctccttcaac aagaaattct actgtacctg cgttgacata cccgacatat ttcatcaatt
    14281 ggacagctgc ctggcagatt ttttgacgtt gctgttcatt catagagaca caaggcgcta
    14341 cttctactac tttttggtga cggcgctgaa cagaacaatc tctttcaaaa agatggatga
    14401 cgtttccatg cgtatccccg agaatctgta cttcgatatg cttaggatta gcgatgtatt
    14461 tttcaacata cacttcatca ctaccaaagg ctgcttttgc ttcgcttttc gcacgttcgt
    14521 agccctctct tgcactcttt tcatcgtgag ctacacgcat gccgcgtccg ccgccgccta
    14581 gagctgcctt gatcataatc ggataaccgt attgtttcgc aaactccaag gcatcgtcta
    14641 tagaagcgat tggtccatct gtgcctggaa tcgaagcaat ccccgcttcg atagctgcag
    14701 cttttgcttt gattttatct ccaaaaatat ctaaatgatg cagtttgggt ccaacaaaaa
    14761 tgatgccttc ttcttcgcac cgttgcgcaa aattcaagtt ttccgacaaa agaccgtaac
    14821 ccggatgaat agcgtcagct ccgcattctt tcgcgataga aatgatccct tcaatatcta
    14881 gatacgcatc aatcggtttt tttccttgtc ctactaaata tgcttcatcc gctttgaaac
    14941 ggtgtacgga atattcatct tcttctgcat agattccaac agttttaatt cctaattctg
    15001 tacaagcacg aaatacacgt acagcgattt cacccctgtt agcaactaaa acttttttca
    15061 tcgaaccact cctacatctt tttttattat aaacctaagc tcttgcgttt ttcatccgca
    15121 ctgatgttga ggacaaagcc gacacaaatc gaaagcatca gcaaactgga gcctccttga
    15181 cttaaaaatg ggaacgtgat ccctgtcaaa ggaataacgc cagtaattcc tccaagattg
    15241 acaaatactt ggatcaaaaa cattgcgccg atcccaatgc acaaaagcga attgaacgga
    15301 tcttttgaac ggatacctac aaggacaatg cgcacaatca taaagaatag caacgctaat
    15361 atcaaaatcc ccataatcac tcccagttct tccactacga ttgcataaat aaaatcggtt
    15421 tgtgcttctt gtaaaaatcc ttttttctgg atactgttac ctaatcccct gccaaatagt
    15481 cctccgttga acatggcata gtagccgttg actagttgat gccctttatt taattcgtct
    15541 gagaaaggat tttggtaaat agcaaatcga ttatagatat actgtaaacg tccgggtaag
    15601 atctttccgt ttgtgatatt gatcagccag atcgtaaaag tacttagaca aaagcctcct
    15661 actcctacaa tcaaagtata cacataattg actccgctgg ctaaaagcag aaccaaaata
    15721 atcaaaaaga taactgccgc attaccaaaa tcgggaagaa tagcgactaa tgcagtaagt
    15781 ccgataacca ttgcaagtgg acgtttcaca gtcccaagaa aatctttttg tacactgttt
    15841 tgtcttttag ataaagtcag agacaggtac cagatagaaa tgatttttag gtattcggca
    15901 ggttggatcg agaatcctgc aatttgcagc caacctttcg ccccattgat ctccttacca
    15961 aaaaacacaa caatcagcag caaaatcgtc aaaactgcga tggctgccat gatcaaacgc
    16021 tggtttttca aaacatctgt tttcatttta taaagtagtg cgatagcaat caagctaagc
    16081 acccaaaaaa tactctggtt aatgactgat gcggacggat tttgtccgtt ttctaataat
    16141 agatacgacg tagaactata taccatgatc aatcccaata cacataaaat taaataggga
    16201 atcaagatgc tatagtctaa tagatgcctt tttttcactt tcttaggcaa gtctattcct
    16261 ccttgccaac ttctttttta tcataaattt cttggtaaac ctcactatat aactgattca
    16321 attctcgttc tagctcactt aacatcagct ggccctcgtt ctttccgatt actcctatac
    16381 ggacagcaaa accgacttgc cgggaaaagc cgtacatctg tgtatctacc acttcttcga
    16441 aggctttgca ttgtgagata cagagactgt tgttttgatt tcggattaat cgtttgatcc
    16501 gttctgcttc atcatttaat acttggatcg caagtttttt cgacaaagaa tccatctgtg
    16561 cttacctccc attctaattc cttaattata acatatttga ttttcataag gagcaaaaaa
    16621 aacagattta accagttgtt tttattcatt tttacgtatt cgttctttct acttatccag
    16681 aacgttagag tccgcatttt ttatccgaga taaaaaggct tcgccatatt tatcaagctt
    16741 gctttggcca actcctttta tctgaagcat ctccaatctg cttgatggac gtattcggct
    16801 catttctttc aatgtggaat ctgaaaatac gacatacggg ggaacgcctg cttctgatgc
    16861 tagatccgtt cgcagttccc gcagcacttc aaatagtgta tcggtttctt catccgatag
    16921 ctgctggact ttcttgggtt cctttcgata aacagattct tgtcctttca atacttgtat
    16981 ccctctttct gttactttca aaacaggata ctcaccattg atggtcagaa gatagccatt
    17041 cgatatcaaa tattcaatta actgcatcgt ttctttttgc gattggtttt tcatcagtcc
    17101 ataggtagat aaatgtccaa aacctaaagc ttgcagcttt tgttctttcg atcctgctaa
    17161 gactttcatc agcatttgtt taccatagtt ctcacccata cgttttaagc aagaaagaac
    17221 catctgtgct tctgtcgtta cttctactat ttctctgtca tctaaacagt taccacaacg
    17281 gttgcacgga ctgcttgtct cgccaaaata gtttaggatg tattgctgaa gacaggactc
    17341 aatatacacg tattctgtca tcgcttttaa tttttcatat tctttttgct tttgcccttc
    17401 ttctctttgg gactgctgga taaaaaactt ttggacttgg atatcttgag gagagaaaag
    17461 caggatggct tcactggaaa gaccatctct tcccgcccga ccagcttcct gatagtatgc
    17521 ttctagagag ccaggaattt gataatggat aacaaaacgg acattgcttt tatttatacc
    17581 cataccaaat gcatttgtcg ctacaatcag ctgcaagcga tcgtataaaa aggcttcttg
    17641 catttctgtt cgctcattct catttagacc accatgatat ctacccacgg aaaaatcgaa
    17701 ttttttaagc aaatgataga gacggtctac ttcttttctc gttgctgcat aaatgatacc
    17761 agattgattt ttgttgatct ttaaatattc gattagatat tgttcttttt tttgatcttt
    17821 aattacttga aatcgtagat tttctcgttc aaaaccagtc tgaatatggt tgttttctgg
    17881 aatttttaaa agtcttttta tgtcagcagc tacttgcaca gtcgctgtag ctgtcaaagc
    17941 aacaattaca ggacgatttg gcagttgatc tagctggttc gccatctgta aataacttgg
    18001 gcggaaatca tgcccccact gagagataca atgtgcctcg tcaatagcga tcatacggac
    18061 gttccatcgc ttcattgctt ggataaaatc cggcatgata aaacgttcag gagcgatata
    18121 aagtaaacgg tactgctgtc tatcgatttc ttggaagcgt ctagctgttt cgtaaccatc
    18181 taacgagcta ttgataaaag tggcgggtat acctaattga ttcgccgcgt ccacctgatc
    18241 cttcatcaaa gagattaaag gggacacaac cacagtaagc ccatctaata gtaaagctgg
    18301 aagctggtaa cagattgatt tcccacttcc ggtaggcatg attcctaata cattttcctg
    18361 atcaatgact ttctggataa tttctttttg accaggtcta aactcatcat agccaaagta
    18421 ttgttttaat aagtcgttca tcttcccgct cctttctctt gccattttac ttcgattttc
    18481 ttcgaaggac aagttgatct cctttcgtga aacattatct tattatgaaa caaaaactct
    18541 ctagaaataa gttttttaaa agtcttttaa acgtctttcg gcgtattttc tgaatttatg
    18601 tccattgaca aagatgactt ctccacagaa aaga
//
LOCUS       DACCQI010000063        18389 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCQI010000063 DACCQI010000000
VERSION     DACCQI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.064"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..883
                     /locus_tag="IVO76_001696"
     CDS             <1..883
                     /locus_tag="IVO76_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7429658.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="IVO76_001697"
     CDS             931..3060
                     /locus_tag="IVO76_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7429659.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="IVO76_001698"
     CDS             3035..3724
                     /locus_tag="IVO76_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429660.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4818)
                     /locus_tag="IVO76_001699"
     CDS             complement(4132..4818)
                     /locus_tag="IVO76_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7429661.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4954..5148)
                     /locus_tag="IVO76_001700"
     CDS             complement(4954..5148)
                     /locus_tag="IVO76_001700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429662.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="IVO76_001701"
     CDS             complement(5198..5638)
                     /locus_tag="IVO76_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7429663.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="IVO76_001702"
     CDS             complement(5642..6487)
                     /locus_tag="IVO76_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7429664.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="IVO76_001703"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="IVO76_001703"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7429665.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8130..9332)
                     /locus_tag="IVO76_001704"
     CDS             complement(8130..9332)
                     /locus_tag="IVO76_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429666.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(9319..11106)
                     /locus_tag="IVO76_001705"
     CDS             complement(9319..11106)
                     /locus_tag="IVO76_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429667.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(11119..12072)
                     /locus_tag="IVO76_001706"
     CDS             complement(11119..12072)
                     /locus_tag="IVO76_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7429668.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            complement(12257..12607)
                     /locus_tag="IVO76_001707"
     CDS             complement(12257..12607)
                     /locus_tag="IVO76_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7429669.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            12807..13886
                     /gene="pepA"
                     /locus_tag="IVO76_001708"
     CDS             12807..13886
                     /gene="pepA"
                     /locus_tag="IVO76_001708"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7429670.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            14029..14349
                     /locus_tag="IVO76_001709"
     CDS             14029..14349
                     /locus_tag="IVO76_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7429671.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            14371..14835
                     /locus_tag="IVO76_001710"
     CDS             14371..14835
                     /locus_tag="IVO76_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7429672.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            15031..15636
                     /locus_tag="IVO76_001711"
     CDS             15031..15636
                     /locus_tag="IVO76_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7429673.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(15675..16964)
                     /locus_tag="IVO76_001712"
     CDS             complement(15675..16964)
                     /locus_tag="IVO76_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7429674.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            17392..17871
                     /gene="rlmH"
                     /locus_tag="IVO76_001713"
     CDS             17392..17871
                     /gene="rlmH"
                     /locus_tag="IVO76_001713"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7429675.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            18074..18352
                     /locus_tag="IVO76_001714"
                     /pseudo
     CDS             18074..18352
                     /locus_tag="IVO76_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5977 a         3371 c         3643 g         5398 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagccgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccaggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtatttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattgtttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaaactc cggctatctc tgacagctgg agtttttatg
     8101 tttaaaaatg aattagtaca tcggtattct taaaagtaat ttagttcgtc ttttgatttc
     8161 tcaggaggga tcaaaaaggc gaaagcacta agtatagcaa aacttgtgat gatttgcgta
     8221 cgatacgtaa accaataatg aatctgtgag tgattagcta aaatattgta ccaaatatac
     8281 ggcgttacag cagctaaaac aaacaaaact agaagttgga acggacgcca gctatttcgt
     8341 ttacgataag caaaatagag aaaaacaaga caagtaatca atagcaataa tattgctact
     8401 tttggataca tcaatctaat attgcttttc agcatctcta atcgatgcaa cgggtatttg
     8461 tcgtttcctt ccgttcgaaa gaggatttgg ttgattgcat cttttacaat gtttttgttc
     8521 aatatgatcg atgataggat ccatttagcc acccatgtca aaccatagcc taatccccaa
     8581 aagataccgg taaaaacaaa actgcctact tgtttgctca atgaactgcg tttatccatt
     8641 aaatacttat ttgttacata ataaataatg atcacaggta tcccccaaga gatgaccggt
     8701 gttgtcaaca agtcaaaaaa gttagctaaa gaaccaatga caaagaaata caagaataat
     8761 tgttggttgg tgttggagag ttgtggtttt gttaaaaata gtaatacaga caataaagtc
     8821 aaaatccaaa tatttgaaaa ttgcatactg aagaaaaagg ttgcagcatt tcctatagca
     8881 agcgaaatca acaaggctgt cgttaaatag atgtcgctac acttggttaa aaagtagctc
     8941 gtcaatccaa tcagcagaaa aagaatggct gcgtaaatca ttcgaatact ccccaaactc
     9001 atccctagca gtaaaggtcg taaaaaaagc tgataaccat gccaataacg gggataatcc
     9061 gccatagaca tcgcgttctc caaggagttc aattcaggat ctgcttttgt ttttcgtatc
     9121 atcaaatgat cagtaaaatt atctaaccga gtgcctagca tatttccttg attgatagaa
     9181 ggatacattc cttctttttc aaccatctgt agagattttt ctgcattcga ttcaatagag
     9241 tgctgcggaa tgaaaaaagt aattgtcagt agtacgcaat agctagcgat caagagaaca
     9301 aaagcaaaaa atatcttttt aaatagtttc atgagtatct cctatatctg ttttcatttt
     9361 ttctctttta tctagtaaaa aaggagagag gatataaaca ggtagaccta atgctaaaac
     9421 aaacacatac cgtaaactaa aagccacagg agtagcaatc ataatagtag caaaaacaag
     9481 cataccggga agaaaaagga gtaaataccg atattttttc ttaaagattg ctaaaagtgc
     9541 actgccgata gctaaccaca gtaatgtgcc ggaaccaaag aaatcacgag catcgatgat
     9601 ttttttcatt cctgacaatc cagtaagatg ttcaatcaaa tctacttgct tcataccata
     9661 attattcgtg tttacacgag tatctaaaaa gccgtaacta ttttttgtac caggtaccca
     9721 gaacccgaac gtttctaaaa tataagcatc tgtatagatt tttatattac ttggtaaatt
     9781 tcttgcccat agtgatagaa attctttttt atgtgtattc aaaaactcat tgttaaaatt
     9841 tttatgccac ttgattgagt ctgcaagact aggagaatac gcttcataat cttttatcgg
     9901 gagtagctga ttcatgaatg ctttatcttt cttagacaag tcaccatcca taacaatagt
     9961 tctagaaatt tgctgcaaag gaacacctaa ttgttcagca gtttctgtag gaatagcata
    10021 acgagccatc aacgggtgga tgagtagtcc agcaatcact gtataaccca gcaccacttg
    10081 aaagaatgct tgataccttt tttgtttcaa aataatcaag ccgcaaatca aacctgtgcc
    10141 ggttactaca taaatcccat tatttcttag ataggtggtc agtaaagcaa ggatgattac
    10201 gtaaatctga ttgagaatcg ttggcttttc tcgttgaaaa ataaagtcaa aaaccgctaa
    10261 ggaaaataaa aagcaagcga tagagaacaa agtatctttt tgcatgttca aggcgtagat
    10321 tggaaaaacc ggtgcaaatg caaaatagca taaactaatg agtaccggaa aaaaatggat
    10381 gtttcttttt aacagccagc aaagagaata acttatcgct gaagctgtta tcaatgtttg
    10441 aaaaatcgaa aataaaaata caccgtgatt gatcgtatca gttggtaaat catgaacaaa
    10501 tagcttgacc agtaaggtga ataagactgg atgatgattg cttataggaa catcacccat
    10561 agattgggcg accgaactta acgaatcagg caaaaccact ccaggataca atgccaacag
    10621 ataaggaagc caacacagaa ctaaaaagac agtaaagaat aaaaaatatt ttcttgaaga
    10681 gaaagcctgt gtgttttgag cagtaaaaaa tgtttccagt tttggcattg ataaataaat
    10741 cgattgtaaa aataaaaaag aatacacaaa aatggagaaa taaacaagaa agtaaaaaat
    10801 acttccatca gcaatcgttg tctttgtata gtttgctgag acatactgaa attgtatctg
    10861 tctgccaata gaaagtgtaa gagcaaaagc tgcagaaaaa aacaatcggg caatagctgc
    10921 aactttgttt tttattgaaa acaactctat tccatattgc ttaaaagcaa gtagcaaaac
    10981 catggctaaa ccaatgatta ctgttggatt caaagttcct agaccataag aagtaaaaaa
    11041 agcaaaacct gacgcaaata aagttgcaaa aaaagtagga aatagattct cctttttctt
    11101 atgcataaaa aaggcctcct acttcatttc tttattttga tattcgtggt agacatggta
    11161 taaattcaat tcatattctt tcttggcatt tgtgacgacc gtatccaaaa tcaaccctgt
    11221 tactaatgaa agaagggcta aagtcatcaa tccagtactt aaaatcgcag aagggatgcg
    11281 tgtaataaag cttgtcagca tatattcacg aatcacagga atccccatga ccaatccaaa
    11341 taaaaagaag aagatggtcc agatcccaaa gaaaagcaaa ggtttataat ctttgcacat
    11401 tttgatgatc atcatgatca ctttgaatcc gtcagagaat gtattcaatt ttgattcgct
    11461 accttcagga cgatcgcgat aatcgattgg aatctcaacg aacttgaatt ttttatctaa
    11521 agcatgaatc gtcaattctg tttcgatttg aaaaccagaa ctcataatag ggaagctttt
    11581 gacaaaaatt cgattgaatc ctcgatagcc ggtcatgaca tcgcgaattt ttgttttata
    11641 taagtaagta attgtatttt taaccagatt attcccaaaa tcatggaaag gacgcttatt
    11701 ttcatcaaaa taggttccat ttgatagacg atcgccaatg accatatctg cttcgccaga
    11761 acgtaatttc tcaagcaaac catgtgcagc ttctgcagga taagtatcat caccatctac
    11821 catcaaataa taatcggcat caatatcaaa aaacatttga cggattacat tcccttttcc
    11881 ttgacgtggt tctttcttta cgattgctcc gccttgaacg gccaattcgt atgttcgatc
    11941 ggtcgaatta ttatcataga cataaatatc tgcttctggt aattcccttt taaaatcttc
    12001 aataacagtt gaaattgtcg cctcttcgtt ataacaagga atgagcacgg caattttttc
    12061 acgtgattcc atttgtcact ctccttatat aataaaacta atcttatttc aataataccg
    12121 tataataact acaaatacta gctattccaa gaggaaatag ccctattttt ataaaaatca
    12181 cccactataa aagaaggata ggatacctct cttttagata gtgggtgcgt attcgttatt
    12241 tcattttttt gacgtattaa tctattgcgc ccttttttct tgaaatgtct aatacggttc
    12301 ctgtatgagc atctgctaaa aattcataat aaacagtttc gccatcttct atccgggaaa
    12361 gcccaccccg ataagcactc gaccgaacgg cgaatttccg aataggttct ttttcaaaag
    12421 agatccaagc accttcgatt gcgccttctt ttaaaaaaga tgttttgata ttttcaagca
    12481 cttggtctgc tgataacact tttttgcgat gataccaaat cgttgaagct accccactca
    12541 ataatcctag accgatccca acggctaacc cgcctttaaa atagcttaat tcgctttctt
    12601 catacatgtg gatatacctc cttcttctca cttgctactc tttgtgttta ttttaccaca
    12661 ctctcatatt ttctttgaaa taaataaccg ctaccagaaa aaataagcta taataaatac
    12721 gatcggtctg cagaaaaaat catattttat ccgcagatca atataagtct gagaggagtt
    12781 tagtctgaaa tttagacgaa tagcatatgg aagaaaaaac atttcaacga atcaaagagt
    12841 taacagaatt acaaggaaca agcggttttg aacaagacgt tcgcgcctat atggaaagtc
    12901 atatggaacc gctggtagat gagttacagt tagatggttt gggcggcatt tttgggctgc
    12961 gccaccatga agaagcggat gcacctcgtg taatggtcgc agcacatatg gatgaagtcg
    13021 gattcatgct gacacaaatc caagaaaacg ggctttttaa agtcgtacct ctaggcggat
    13081 ggaaccctta cgttgtttct gctcaacgtt ttacacttaa aacctctact ggtaagaatt
    13141 atccatgtat ttcttcttcc gttcccccac atttattacg aggaacaagc ggacaaaaat
    13201 cagtagaagt aacagatatt ttgttcgatg ctgggtttga atcaagagaa gaggcaatga
    13261 gttacggtgt tcttccagga gacacaatcg ttccttacgc tgaaacgatc aaaacagcca
    13321 acgggaagaa tatcatcagc aaatcgtggg acaaccgcta tggctgtaca atggtattag
    13381 aagctttaga agcgttacaa aatgaaacat taggtcatac attgattgca ggcgcgaatg
    13441 tccaagaaga agtaggcttg cgtggttcta aagcttctgt caataaattc aagccagatc
    13501 tgttctttgc agtagactgc tctgcagcag atgacacagt gacgaaaaat ggcacattcg
    13561 gacatttagg agaaggtaca ttgatgcgta tccaagatcc aggtttgatc atgcttcctc
    13621 gtttgcgtga gtacttgcta gatatcgctg aaacaaacaa catcccttat caatactttg
    13681 tttcaaaagg cggtacagat gcaggtgctg ctcatacaca aaatgaagga atcccaagta
    13741 cagtgattgg cgtagtggga cgttatatcc atacgcatca aacgatgttc agtattcgcg
    13801 attttgaagc tgcacgagaa atgttgattc aaacattgaa aggtttagat aaatcaacag
    13861 tgaacacgat cgtatacggg aaataacgcg atagtgaaac tataaaatct ggcaaaagca
    13921 accttttgtc agattttttt tataaaaaga ggatcaatgt ttgttagttc gaaagaaagg
    13981 tgtataattt aattgaaaaa tacataaaca gaaagtagag gaaaaagaat gattatccca
    14041 aatagtttag aagaattagc aggctacgta gaaactggaa aaagtgtatt tttctttact
    14101 gccggctggt gcggagactg ccgttttatc aaaccgcaaa tgcctgaaat cgaaaatgac
    14161 ttttctgatt ggcaatttat tgaagtagac cgtgacaagt acatagatgt agccgctgaa
    14221 tggaacattt ttggtatccc aagttttgtc gtgatccaag acggaaaaga attaggtcgt
    14281 ctagtgaata aagatcgtaa aacaaaacag gaaatcgaat cgttcctaga aagtgtaata
    14341 aaaggctgac atctgttagg aggggtcaag atgaatcaag aatataagaa cattttagta
    14401 ggtattgacg gtagtgaaca agcacttgct gctttcaaaa aagcgttaga agtggctcgc
    14461 cgtaataatg ggaaagtata tgtagcaaac gtaatcgatc agcaatttta taattttatg
    14521 ggctatgctc ctattgatca aaatctagtc gatcagcaaa ctgaaacagc caagcaaatg
    14581 atcgaagaat gtaaagaata tggaaaatct gttgattaca caaatataga aggaattatt
    14641 gcatacggct ctgttcgtga atccatggca gtcaaattgc caaaaaaata tgcaatcgat
    14701 ctaatcatgg ttgggcagtc tggactaaat gcagtcgaac gctttatgac aggaagtgta
    14761 gccagctacg tcatacgtca ttcaccttgt gatgtcttga tcgtttctga cgaagaagaa
    14821 cagccaagta attaagcaag tggaacttgt ataacctttt taatcaaaca gtaaagggac
    14881 tgtgacaaaa gtcttgtcac agtcccttgt tacgaataaa tggtgttcca gaagaagctt
    14941 cttcggaaat aaagcatgaa aaaaatctca tgcagtgaat gcaatttttt gttaagataa
    15001 atctcagcaa aggataatgg aggaaagaac atgatttttg cttataacaa agcacacgtc
    15061 ggagacactt tgatggttat catagccgac gataaaggaa cagaaaacac tgtcgaacgt
    15121 aaatggaatg ttgctcggat caaagatgaa caaggtcaag ttgttgcttg gaactttttc
    15181 catatctccg atcatctaac aattgaaggg aacggccaag taagcttaac agatgaagaa
    15241 ttagcgaaat tgaatcgatt gattaaagaa gcaggattcg aagaacagct tgaagctgat
    15301 catgaaccga agatcgttgt cgggtatgta aaaacatgcg tcccgcatcc tgactctgac
    15361 catttatcga ttactgagac agaagtggac aatgggacag tattgcaaat tgtttgcggt
    15421 gcaccaaata tcgaagctgg acaaaaagtt gtcgttgcaa aaccaggagc catgatgcca
    15481 gatggaatga tgatctggcc aggaactttg cgaggggtag ccagctatgg aatgatttgt
    15541 tctgctcgtg aattgcattt gccaaatgct ccagaaaaaa aaggaatctt agtattgcct
    15601 gaggatgccg tagtcggaga agtatttcca aaataaagag gttgtgacaa gtctttgtca
    15661 caacctcttt tttattaatt ttcactattt tgattttgtt ttaatgccga ttgatcgatc
    15721 gtcaattcaa cagtagttgt tttttctttc gagccttcat agtaagtgat tttgacagaa
    15781 tctcccactt ttttcttata caaagcagat tgaagttcta caccagaaga aacttctttg
    15841 tcatcgatct tagtaatgac gtcgtattgt ttcaacccag ctttttctgc cggtgtagct
    15901 gtttggacat ttgtcacgat gacaccgtta gttacagatg aaggaatctt caagatttgt
    15961 tcttgttgct gagtagaaac ggcagataag tcgaccattg tgatacctag agctggacgt
    16021 gtcacttttc cgtctttttc cagttggttg atgatattca ctacatcgtt acttgggatg
    16081 gcaaatccca ttccttctac gcttacatta gaagtcgatt cagaagtact tgcgatctta
    16141 cttgagttga taccgataac ttgtccttcg atattcacta aaggaccacc agaatttccc
    16201 gggttgatcg ctgcatctgt ttggatcgca ttgatattga ctgtttcgtt cgattcattc
    16261 gtacttgtta cttgtcggtt caaggaagaa atgattcctg aagtgacaga attcgcatat
    16321 tcagatccta atggggaacc aatcgcaatc gcaggttcac caaccttcaa agcacttgag
    16381 tccccaaaag aggcaaccgt ttcgactttg tctgaattga tcttcaacac cgctaaatct
    16441 gaataggcat ctgttccaac taattcagct tttaccttcg taccgtcttt catcatgact
    16501 tccaaacctt gctgtccatc tactacgtga ttgtttgtga ctacataagc agtattcccg
    16561 tcttttttgt agattacgcc gcttccttca ctataagctt ccaaattact atcatctgag
    16621 ctgctacttt cttcttgctg cccgaataat ccgccaaaac cgcttgattg gttttgactt
    16681 tgtaagttga taacagaaac gacagcgtct tgaactttat ctaccgcttt ggtgatatca
    16741 gaatctacat tgactttgac attttccaca actgtttcac cggcagaatt ttgattgccg
    16801 cttgtagcag tagaactatt gttgccagtg cccatcgctg cataaaaaat gccagctgtc
    16861 agtagtcctc caacgatccc tccaacaagc cccagaccca acttacgcca gaggctatta
    16921 tttttatttt ttttcatctt tggtgtcaca ttttttctat ccatgtgttc ctcctccaat
    16981 tacattcatt tatctactgt tagtatagtc ttatttgttg actttagtct actttgagac
    17041 tttgaacttt ttatgaaaaa ctttaggaaa aatcattgtt ttcagatgaa aatatttgat
    17101 tttctttttc cacagatatt gtgtaaaact acgcggatag gattggaaaa ataagggata
    17161 aaacatatcc acagcctgtg tataaagtgg ataaattctt ataaaacgta tgtttgccta
    17221 atgaatgtga tgaaataagg taaaacaacg ctgttttctg tggataaacc tgtggatgtt
    17281 gtgcataact gttggggaaa tctttttaac aaaaatagtc aaaataaaca tatagacgaa
    17341 aaaattgatt ataataaaca aagtgagcgt gaagatcaaa ggagagaaaa catgaatatc
    17401 aaaattattt cagtaggaaa attaaaagaa aaatacttga tccaaggtat caatgagtat
    17461 gtcaaacggc tgaatgccta tgcgaaaatc gaattgatcg aagttcctga cgaaaaagcc
    17521 ccggagaatt taagcgaagc gcaaatgaga caagtaaaag aaaaagaagg agaacggatc
    17581 ttagcgaaaa tcaaagaaca agaatacgtc tatggcttag caattgaagg gaaaaatccc
    17641 acaagcgaat cttttgctaa acaaatcgac caattaggta tccaaggaaa aagccacctt
    17701 gtttttgtta tcggcggttc tttaggactc agcgaagccg tcatgaaaag aagcaatgct
    17761 cagatctctt ttggcaaaat gacgtatcca catcaattga tgcgcctgat cctcgtcgag
    17821 caaatctacc gtgccttccg gatcaacgcg ggggctccat accacaaata atgatatttt
    17881 ttaagagaaa aaattatccc tattttaaat aatagcaata ggtagtggta gggagaaatt
    17941 tataaataac ttttttattt gctttggtag ccaagtaagt tgcaaaagaa aaatagatac
    18001 tgttaaaaag ataatgtaga catagaaaca aatacgtaaa attttagtta gaataatggc
    18061 aaggagaaaa aacatgatag atgaaaaaaa gtgtttaaaa aatctattgg aggatatgga
    18121 tgatattgaa gaaaaactat ctaaatggac taggaaaaca aatatatttc agatacttaa
    18181 gctatctaaa aatgaaataa gacatagtaa ttttttagct tttttgtttg ctccaaatga
    18241 aacccacaat ttatctgatg agttttttaa aatgtttttg aaaaggtata tagattctaa
    18301 tgatgatacc aaagttgcta tcaattattt tgatgcttta cttaatagct acccgtggtg
    18361 ctagttaatt ttaatatcct aaagaatat
//
LOCUS       DACCQI010000064        18105 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCQI010000064 DACCQI010000000
VERSION     DACCQI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.065"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(11..1519)
                     /locus_tag="IVO76_001715"
     CDS             complement(11..1519)
                     /locus_tag="IVO76_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7429676.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1678..1869
                     /locus_tag="IVO76_001716"
     CDS             1678..1869
                     /locus_tag="IVO76_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429677.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2072..2989
                     /locus_tag="IVO76_001717"
     CDS             2072..2989
                     /locus_tag="IVO76_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7429678.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            2982..4055
                     /locus_tag="IVO76_001718"
     CDS             2982..4055
                     /locus_tag="IVO76_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429679.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4203..4721
                     /locus_tag="IVO76_001719"
     CDS             4203..4721
                     /locus_tag="IVO76_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429680.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4702..5094
                     /locus_tag="IVO76_001720"
     CDS             4702..5094
                     /locus_tag="IVO76_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7429681.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5170..6105
                     /locus_tag="IVO76_001721"
     CDS             5170..6105
                     /locus_tag="IVO76_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7429682.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6238..7017
                     /locus_tag="IVO76_001722"
     CDS             6238..7017
                     /locus_tag="IVO76_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7429683.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7010..7327
                     /locus_tag="IVO76_001723"
     CDS             7010..7327
                     /locus_tag="IVO76_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7429684.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7571..9181
                     /locus_tag="IVO76_001724"
     CDS             7571..9181
                     /locus_tag="IVO76_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7429685.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9338..10186
                     /locus_tag="IVO76_001725"
     CDS             9338..10186
                     /locus_tag="IVO76_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7429686.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10417..10896
                     /locus_tag="IVO76_001726"
     CDS             10417..10896
                     /locus_tag="IVO76_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429687.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            10929..13157
                     /locus_tag="IVO76_001727"
     CDS             10929..13157
                     /locus_tag="IVO76_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7429688.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13286..14929
                     /locus_tag="IVO76_001728"
     CDS             13286..14929
                     /locus_tag="IVO76_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7429689.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15002..15547)
                     /locus_tag="IVO76_001729"
     CDS             complement(15002..15547)
                     /locus_tag="IVO76_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7429690.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15570..16055)
                     /locus_tag="IVO76_001730"
     CDS             complement(15570..16055)
                     /locus_tag="IVO76_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7429691.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16045..16857)
                     /locus_tag="IVO76_001731"
     CDS             complement(16045..16857)
                     /locus_tag="IVO76_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7429692.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(16996..>18105)
                     /locus_tag="IVO76_001732"
     CDS             complement(16996..>18105)
                     /locus_tag="IVO76_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7429693.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         5879 a         3299 c         3626 g         5301 t
ORIGIN      
        1 taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg caggtaatat
       61 cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca gtagagtggg
      121 cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt ggattcgtta
      181 ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc aaagaggaat
      241 gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac aataaaggaa
      301 tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc gctgacgcat
      361 ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg attgcaatca
      421 cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag tcatgggcag
      481 ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc agtttgattc
      541 gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt tggaaataac
      601 gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg atcccaaatc
      661 ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta ccagtaattt
      721 tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag ttcacagcta
      781 ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt agatcgactg
      841 tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat actgggaaag
      901 tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac agttggttca
      961 aggaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg attggtttag
     1021 ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt tctacgttga
     1081 ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt tctagtttat
     1141 tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca ctgttgatta
     1201 ataagccata accacgtaat tcatcgatta accgcaagat atccatatca taagtaatcc
     1261 cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa atcagtattt
     1321 cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca aatcctggaa
     1381 gtacacgttt cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa tataatttgt
     1441 catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat ttttctgaat
     1501 caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt ttcatttttt
     1561 tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa tggtttaaac
     1621 caacttgtct agatagttat tcattggtat gatagttttt gataaggaag gtgctacatg
     1681 aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat ttttaccggt
     1741 tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta tctaaacgaa
     1801 gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag agatctgcct
     1861 agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca gatggaaaga
     1921 ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta tggagcattg
     1981 cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta ttaataagtg
     2041 aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa atgcaaatta
     2101 tgagccagac gaactagagg agacgaatgc ggatcaggat acaggacaag aatcgtcttc
     2161 tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag aagacttaca
     2221 ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt tatacaatgg
     2281 ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg atcaattgct
     2341 gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac aagtagaaaa
     2401 catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc ggcccacggt
     2461 cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg aagagtggga
     2521 gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc tagacgcaaa
     2581 ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag aaatcaatcg
     2641 acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa tcgaggaaga
     2701 aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag gctggcttcc
     2761 ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc aagaagcaag
     2821 agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca gacgctattt
     2881 cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt atcttggcaa
     2941 tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa cacgatgaag
     3001 cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac acttcattgg
     3061 aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta gaggaaacaa
     3121 aaatacacga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa aacacccatg
     3181 cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat catcaagata
     3241 tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg agaaaagatt
     3301 tgatcgaatc agattttcca caattattag aatcatatga tcgttttata ttgaatgaag
     3361 aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat gaattactga
     3421 aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca tggaaaacaa
     3481 gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt tcctttgtat
     3541 taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac gtatcaccag
     3601 agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga cactggtcag
     3661 tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg aatcaattat
     3721 tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac aatacgaagc
     3781 cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg gatgatattt
     3841 ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa actacttatc
     3901 aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa gatcaaacag
     3961 tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt atcaattacg
     4021 aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat taccagatgt
     4081 cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat tgtcacaagt
     4141 cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg atttcattag
     4201 gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt gtagcaggta
     4261 ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt ttatttatag
     4321 tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt gcgatcagca
     4381 gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat actgttgttc
     4441 tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta tggttaaagg
     4501 gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt tttgatacgc
     4561 tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg tttgatttga
     4621 tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc aaacgttttt
     4681 acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc aatgagtgtc
     4741 agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc caaccaaaag
     4801 gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg tcaaaacttt
     4861 tcggaaaaag aagtgttctt caaaaaaggc gatgatttac tgagtatttt gaaacgaaat
     4921 tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc acaggatgag
     4981 aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac tggtgccaaa
     5041 gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact ataagtttca
     5101 taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg atgaaagaag
     5161 gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc tgctctaggg
     5221 gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg cagtgccgcc
     5281 atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt gctgttattc
     5341 ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga aggacgagca
     5401 aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg ggcacttggg
     5461 gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa tccgatcatt
     5521 gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg cgtggcaatg
     5581 aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt gcgcgaaagt
     5641 cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat cggtttggtc
     5701 ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt tgatgcgatc
     5761 agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc aaaagaattt
     5821 tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat caagttagct
     5881 tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt aagtccaacg
     5941 gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga agcttactcg
     6001 gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa gatgtatata
     6061 tacattgaaa cagataaata tgacccaaat tattctcgta aataaaacgt ttattcatga
     6121 attgaagcaa aaatgagaaa aatataatct tgtttttaaa taaaacggct acaatttagc
     6181 gaatttatgt tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga tattttaatg
     6241 aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc actttgtata
     6301 agctatatac cagtaggatt agcttgcgga gtattgttgc aaaaagttgg atttgatcct
     6361 ctcttatcag gactgttatc gattctggtc ttttccggtg gcgcgcagtt tttagttgca
     6421 tctatgctta cgacgcaagc atcatttgca acgacattat tgatggtttt ctttttagaa
     6481 ttacgctata cattattagg agcaagtctt gccggtttta tgaaaaaaga aaagcggacg
     6541 tttttggctg tcttttctca atcactgaat gatgaaaact atgcggtgaa ctacttaaag
     6601 ttttcaactg acccttcctg gaataaaaga aaggctttat atgtcaattg gttctcaatg
     6661 actgcatggg ccggtagcaa tatgatcggt aattttttcg gctcggtgat tcgagtagat
     6721 gctgaccttg tccattttgc tttgacggcc atgttcattt tcatgtttat catgcagatg
     6781 aaaagcgcac tactgatctt tacaggttta ttctcaggcg tattaggggt cgtattcatg
     6841 gtattattcc aaaatacttt cggattgatt gcagcaacag tcattgcttc atttgccgga
     6901 tttatgctgg agaaggcatt caaaaaagaa aaagctaaga aactaagaga ccgcggtaaa
     6961 gatgtcaatg agccgctctt caacttcccg gaacaggagg ccaatcacca tgactaaact
     7021 atatttgcta cttttagttg cttgtctgtt catcgtggca tatatcccgc gcttgttccc
     7081 gatgctttat tttactcatc gaaaagtgcc atcctggttc agtgactgga tgaaatatgt
     7141 accggtggca ttatttgcgg cgttagcttt taaagatgtt ttcattactc atgaacatct
     7201 ggatattgct tggaatatca aaatagctgc aatgattcta gtagcaggcg tggcttacaa
     7261 aacacgttcg atggcgttgt cagttttgac aggacttgcg tctgtctttt tgctgtctat
     7321 gctttaaact gttttatttt attaaaaaca gagattgcga cataagttgt tcaaactgat
     7381 ctatccgaac tgcatttgca acagtcaatt ctggatgcaa gtttattcgt agggataaaa
     7441 aagattagtg ttggcttatg atgcaagctc ttttttattt taggaattag agagggattt
     7501 gtccttctat gattttttgc tataataggg aagatgttta ttttatgaaa gaatagttga
     7561 gggaatttaa atgaataaaa aattgatgca aggaacattt tggctaacct ttgctaatct
     7621 tctatgtaaa gttttagggg tagtctattt gattccctgg ctaagtatga tggggaacaa
     7681 tcaagatggt atgcttgcta cgacattgta taatgttggc tatcttcctt atggattgtt
     7741 cctgatgctt ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag ctgtagccac
     7801 aaaagaagga gataacgctg cttgtcggct gatttttaga agtacgatca atatcatgtt
     7861 tgtcatcggt atcgcctctg ctgcactgat gtatttattt gctcctttac tagccggaat
     7921 cagtccgatt gctaacgtta ataatgggat actagctatc agaagtttat gcccatcttt
     7981 gattgctatc ccaattctta gtgcaatgag aggatatttc caaggaaaaa atgatctgcg
     8041 gccttatgga acttctttga ttatcgaaca ggctgtgcga gtgatcgtta tcttggctgg
     8101 tacttattac ttgcgcgtat tgactgacgg aaccatatta gaagctgtat tgatcagtac
     8161 agtcgcttct ttcttcggtg ggttagctgc gattatccat atgtatatcg tcggacgcag
     8221 aaaagacttc ttcgaactta aagatttttt gatttccagc cgttatttcg aaagagaaaa
     8281 ccgatctgcc tctgtctcta tcattcggga aacactaccg tttatttttg tcggctcagt
     8341 cattactatc gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc actttttccg
     8401 tccagagatc gcagatcagc aactggaatt cttattcagc cgtgcttctg tcaatccaaa
     8461 taaattgaca ttgatcctga tctccatggt aggaacagta gctataagca gcttgccaat
     8521 attaagcacg atgaagaaaa aagatcggat acaaatcgaa aaaacagtag gagatagttt
     8581 ttcgattgcg ttattgattc ttcttccttc attgactgga atgtctttat tagctggacc
     8641 actatacaca ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc aaatggcttt
     8701 gctagcttct ttgtttttct ctttattcac gatcctatct acgatgcttc aatctttgaa
     8761 tcaccatcgt tttgcgatcc gattaactgt agaagcaatt atcctgaaag tcatcttcca
     8821 agtaatcgga ttaggattag ttggcggata cgggatgagc ttatcaaatg gcttagcatt
     8881 cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa tagcaccttt
     8941 agcaaagatc agcaattttt tcttaaaaac tttccgctcc accctgatta tgcttgcagt
     9001 atgttttgcc gttttctttt tgctgaatca gcgattgtca atggaatcaa aaagctatgc
     9061 aatgatttat tgtgtggctg taggaagtat tggcggctta gtatttttat tttctcaatt
     9121 cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa gcaaagaata
     9181 ggttaactaa tcgttttttg ctgtcacttg aaaaacggca actcgtatga ttacgcttgt
     9241 cgtttttcta ttttccaatt ttagtttcat atctaatctt acttatgata tagttataag
     9301 gtctggaaaa attctttgaa acgaggaaat taaacacatg aaacttgcag tagttacaga
     9361 tagttcggca tatctgccgg aacgtatcaa gaaccatccc gacttattcg ttattccaat
     9421 ccctttgatt atggatggga aaatttataa cgaaggaatc gatatcgaag cggatgaata
     9481 ttatggactt ttaaaaaaca gtaaagattt tcctactact tcacaacctg tcgtagggga
     9541 agttttgggt ttatatgagg aattgaaaga gaaagggtat gacacgattt taagtatcca
     9601 tctttcttca gggatttctg gctttgtcaa cacattattt gcgatgaaag atgacatcag
     9661 cggagtaacg gtcattcctt atgactctaa gatcactagt atgccgatgg gacacatggt
     9721 agaagctgcc ttagacttaa atgatgaagg tcagtccctt gatgaaatac ttgcacatat
     9781 cgatcgaatc cgagataata cctacgctta tctgattgta gatgatttga acaatttagt
     9841 acgaggagga cgtttgacaa atggtgcagc gctgattggc agcttattaa aaatcaaacc
     9901 gatcctgaca ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt caagtaaaaa
     9961 agcatttgct cgagctgaag aaattattgg tagaagaaat aacgaaatac aaccgccagt
    10021 caaactttac gtgatacatg caaataattt agaagtagcg attgcggaga aagagaaatt
    10081 gcagaaaaaa tatccaaatg cagaaatcga aattggtcat ttcggaccag tcattggtac
    10141 acatttaggt gaaaaagcca tcggcattgc catttcagcg caataattcc ttttattcaa
    10201 aaaacatacc aaaaatattc caacatcatc ttggactttc tacatgttac taatgagcgt
    10261 gtaaaagaac gagatggtgt ttttatttct atcaatgaag ctctttttct ttgcaatcct
    10321 cacttcaatc ctctataatg tcgcatatag tttagtaaat gagtaaacta ctaatttact
    10381 tatttactaa aaataaaagg atgaggagtt aaaataatgg aaataccaac aaatttaaaa
    10441 cataaaccag taatcgttgt agaagactat gataaagttg atggaagaaa tgcattaaat
    10501 acggatgcaa aagggctttc actgggcctt gctcagtgga atgatcgtgg aaaagtcgat
    10561 atctcaggca aggtatggcg acatactgga gaaaaatggt cacgacagtc ggaagaatta
    10621 ccaatcacac gtattttaga tttagcgatc ttagctgcac aaggaagttt atacttccaa
    10681 gatgcttatc gttatgaaaa attttatgat ccagaaaatc cagtcgtaga catcattgga
    10741 ttacaaggga accgaatgac ggtagaagta gataccaaaa atccaaagat cgatgaagac
    10801 atcattttgt attacgatac actacaaaaa gacggagaac tgattgggga acgattccgt
    10861 attttaaaac gtcttttaga tgagctagga tattaataga cagagatgat caaaagggga
    10921 agagaagcat ggagaatttc tcactaacag aaaaccgaac cttttcacgg ctagaaaagc
    10981 aaatgatctg gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg cggatcgcct
    11041 ccgaaatcaa agggaattgg caagatcctg aaatgaaaat cagtaacgta cttttggaag
    11101 gagatgccgg gtcggggaag acacaattag ccaaagcact atctgctgat ctgcaacttc
    11161 cctatacaaa agtgacctgt tttgcagata tggacaaatc agatgttttc ggagccttat
    11221 tacctgtagt agattctaat aaggaacaag accaagaatt actagaagct atttaccaaa
    11281 cagatacact tgaagctgta ctgacactga tccagaacca ttacggcatt gatccttttt
    11341 ctgctaaaga aaaattagca gagctagttc aaaggatcga agctgattat ccagatgcaa
    11401 tccattacag ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga tatttattag
    11461 agattcaaga gccgactgtc atccgagatg cctctgttct tgtggcactt aactcagcgc
    11521 tcgaaccaaa tgggatgttg aatttaccga caggaatcat tcgtcgccat ccagattgcg
    11581 tcattgtgat cactacaaat cgaaattacc aaggaaatcg cccattaaat gaatctttaa
    11641 gagatcggat gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc atggcagaac
    11701 gtgcgatggc gaaaactatg atccaaaaac aagaacttct cttaaagatg gcagagatta
    11761 tccgtttgtt ggatgaaact gcaaaagcta acgccatcaa aggtgtagca ggaatgcgtt
    11821 cttattttta ttgggcaaat acctataagc aaggacaaga tttactccag tcgatttatc
    11881 caaaagtttt gtataaatta tccactgatt cagatgaatt ggcaattctt gaacaagctt
    11941 tgtctgacag tggattgcta ctggaacttc aggacatatt gagaaaagaa aaatggggtc
    12001 atcctcaatc tgagcataca aaaggaagaa ccattagcgc cgaagaagca aatgaacgaa
    12061 acatcactga aaatgctgaa gaaattctgc gcttagatga aaagaaatcg ttgtctgctg
    12121 aagaaccaaa tgagggagaa gagaaacaag aagaaacaat agaaaaaaac gagattcctc
    12181 ctcaaatgca aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa gaacaacaaa
    12241 caggagatga ttctcaagaa atgtctgctt ctgacatgga agcacatgat aaggaaatca
    12301 agaagcaact gaataaagaa gcgcgacaaa tcatgaagga aacgattcat gaaaaagaag
    12361 gtctcatcgt tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt gagctacaca
    12421 tgcaagtaat gccaatagta gaatcactta gccggcaaat tttagagtta ctggataatg
    12481 aacaaacaga gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat gcaagcaaag
    12541 tagcatatgg tgatttgaga aattttgaca aaaagaaccc gccacatgag caaccttctt
    12601 tagcggtagc tgtacggatc gatgaatctg ggtcaatgat acgggatgat cggatcgaag
    12661 cggcaaaaaa agcagcgatt gccattgccg aattttctaa gaaagtcgat atcccattgt
    12721 tgatttacgg agatacagca gaccgttcgt caagagaaaa gacttctttg ttttcttata
    12781 aagaatttga agacggattt caatggttaa atgaaaaatt agtcactatg aaaccaaggc
    12841 aaaacaatcg tgatggtgct gcacttcgtt tagcagcaga aaaattaaac agacaatccg
    12901 caacaaccaa actgttgctt aatattagtg acggtcagcc aaaagcgttg ccggattata
    12961 caggcaaaaa agcaaaagaa gatatccagt ctgttttgaa agaatatgaa cgtcaaggaa
    13021 tcctatttgt ctccgcagct attggccaag acaaagaaga aatcaagtcg atctacggtg
    13081 aaagtcgttt tgtagatatt actgacttga atgaatttcc caagcaaatg attcaactga
    13141 ttgcccgcta tctatgataa aataaagctc aacgaacgca cttagcagat tcgttgagct
    13201 tttcgtttct agttaccggt aacaaggata cttctaaaat agaatcggtt ataatcaaga
    13261 gggaccgtta aaggagtgaa acgatatgat acaagaacaa aaatggtgga aagaagcagt
    13321 aggctatcaa atctatcctg ctagtttcaa agatagcaat ggcgatggtc aaggagacat
    13381 caatgggatc cgtgaaaaac ttaattattt gaaagatctt ggtatcggct ttatctggat
    13441 caatccgatc tatcaatctc catttgtcga taatggttat gacgtaagtg actatcaaga
    13501 tatagaagag agattcggaa cgatggaaga ttttgattta ttgctaaaag aagcacatga
    13561 attagggatc aaagtgatta tggatcttgt catcaaccat agttcggatc agcatcaatg
    13621 gtttgaagaa tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca tttgggtcga
    13681 cggggtagat ggcaaagaac cgaataattg gacatcgatt ttcggcggtt ctgcttggga
    13741 gtattctcat gaaactggac agtattatct gcatgttttt gcaaaagaac aaccagactt
    13801 aaattgggaa agcgagaaat tgaaagaaga actattcaat atgatccgat ggtggttgga
    13861 taaagggata gatggatttc gtttagatgc gatttctcat gtaaaaaaag atgaatattc
    13921 agtaaaagcc acagagaacc ctttctcacc ctttcaaaat gtctcaggaa tcgaagaaca
    13981 tttgaccgat ttgaaacatg tttttgaaga gtacgatatc atgactgtcg gagaagcaag
    14041 tggtgtcact gcagaagaag gaccacaatg ggtagggaaa gacggttatt tcgatatgat
    14101 attcgaattt gatcatatcc atatctggca gcaagaaaaa gaagggcaat tagatgtcct
    14161 taaattgaaa cttgcattaa gcgcttggca aacttcattg gatggtatcg gatggaacgc
    14221 tttatatatg gaaaaccatg atgttcctcg tgccgtttca gtgtttggag atactcgtcc
    14281 cgatttttgg gctatgtctg caaaagcaat cgctatgatg tatttctttt tgcaaggtac
    14341 gccatttatt tatcaaggac aggaaatcgg aatgacgaat atgccctttg aatcgatcga
    14401 tcaagttgat gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg aaggtaagac
    14461 aagagaagaa gcattggata tcattcgtga aacaacgaga gacaacagca ggacacccat
    14521 gcaatggact tcggaacaat atgctggttt ttctacacat gaaccttggc tgatcactaa
    14581 tccaaatacc aaaacgatca atgtagaaca acaagaatat gaaccagagt cggtcctgca
    14641 attctataag aacatgatcc gcattagaca aaccaataaa ggtctgatat acggttctta
    14701 taaagaatat cttcatgaac atccacaact ttatgtctat gaaagatatt tagaagatga
    14761 acaatatctt attatggtaa acttaacaga atcacttgct gactatgaac ttccaaaaga
    14821 agcagatcag tcctggacat tactcttgtc aaattcctct agcggagaat ttgaagctaa
    14881 aggcatacta gcaccttatg aagctcggtt atataaaaca aacaagtaaa acttcctcgt
    14941 taaagttcat tagttggact ttgcgaggaa gtttttcaga ttgctctgtc aattactcat
    15001 ttcaataatt cgctctagga ccaccgatat atttaggacg agagcgaagg gctgtcacgt
    15061 gagcttgtcc aagggttttc aaacgaggtc tgacaggtac ttggacatgt ttgatatgca
    15121 taccgattga tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt tccacttcga
    15181 caggatcatt gaactggtca aaagcagcaa cagaacaagc acctccagca tgtagtgcag
    15241 gaacaacaga gacaatttcg aaattttttg cttctgccac tgttcgttct actactaatg
    15301 ctcggttcaa atgttcacaa ccttggacag ctagcgcaat ttctttagga tccaacaact
    15361 cttttaacgt acggatgatc cattgaccaa cttccgcact ggaatttttt ccaatatgtc
    15421 ctccaacgac ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt ttcaagtgtg
    15481 cctcagccaa tatgtcatta actatttcag tcaattggtc ttttaattct ttttctgaca
    15541 acttcatttt ctaaaacctt ctttttcaat taatattgct tccgtgcttg aatacgttga
    15601 agacttgcag ttataggaaa ggcaagtccc attccaatca gattttggaa gatgtttcct
    15661 ggtatagaag ccactccagc cggccaatca tataaaatcc aaccggcgat accatagccg
    15721 atgatcataa ctaagcttcc taataaaagc cctaatcccc acatacgagt agaagatttg
    15781 ctgaaagaac cagcaacata tccttgtaac ccgtggatca ctaatgaaaa aactgcccac
    15841 tgcggatatc ctgaaatcaa atcgatcaat agaccactgg aagcaccaac aactaatcca
    15901 cccgttggac cataaagaaa agcagcggta tagatcccgg cctcgcataa tgtcacaaat
    15961 ccattcgtag ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt caacgcaatc
    16021 agcatagcat agatcgttaa ttgcttagtt ttcatttctc attcctcctt tttgaatacc
    16081 gccagaccag acacttccgg tatgatcgtt catcagcacg ccattagaaa tacaatgggc
    16141 aacaaatttt ttactttctg ctactgcttt ggtaaaatct gcccctaaca caagattcgc
    16201 tgttattgca gaagaaaagg cacaaccggc cccgtttact gttgtagtgt cgattttttt
    16261 atgagaaaat acctcataat gatcaattga atagagaaga tccacagctt catctccagc
    16321 catccttgtg ccgcctttga tcacaatgtt ttttgttcct gaagcagtga gttctttagc
    16381 gagatggatc atttctgtta acgaagaggg aagagcttga ccagaaagta aagcagcttc
    16441 tgccaaatta ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga gctgcgaaat
    16501 atacaattga ttgatcacag cttcagtttc tttgaatgcc aaaacagggt ctaaaatgat
    16561 tgggatattt tgttttgttt ttatgaagtc tcgaacaacc atgattgctt cacttgaatt
    16621 caataaccca attttgatac catctaatga agtcatttta tcaatcgaaa ctaactgtcg
    16681 tttaagcaat tccaaagata tagattccag cataaatcgc tcattaatat ctagtgcccc
    16741 tatagaagtc aaggcgctta gtccgaatag accaaaattc tcaaaagttt ttaaatctgt
    16801 ttggattcct ccaccagcaa atggatcaga accgccaatt gtcaagattc ttttcataac
    16861 attttcctcc atgaataaga gtataacgaa attttcaaaa aagaaaaaca gccaattggc
    16921 atattttcat ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa aatggatctt
    16981 tctttcctta aatagttagg acttttgtga ttttaattct ttctcgattt gaagccgttg
    17041 cactttcatt gtagctgttc gcgggattgc atcaaaatca cggatgatag ggacgttcaa
    17101 gcgaggaagt tcatggactt gctcccacca tcgattccaa tccatttctt tatcaggagc
    17161 taaagcaata atcggttgtg gagaatgatt tttatccctt acaatcacta cttctgccaa
    17221 aaaatctaac gaatctaata aaaagtcttc aatagctaat gtactattga tcgtttcgat
    17281 caaatccact tgccgatctt tcaaaaatag gtggccatgc ttatccataa aaccgtaatc
    17341 tccgctatcc caccaatcgc cataaacatt ttcctggaac cgtgcatctt ccttgtaata
    17401 agtcaaagcc cgtcctttag acagtaattg tatatgacca tctgtgtttt caggtaggac
    17461 attcccaact gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa cgcccatatc
    17521 tcttgcatca cttgttttca aactctctag tgtatgagcc ttaagaatca ttggtccgca
    17581 ttcactttgg ccatatactt gaagaaatac cgcatcctgt gccatcgacg ttcgcaaaaa
    17641 tgtcttcatt gtctggttgt ttatagcatc aaaagtggag tgatagtatc ggatacccga
    17701 aaaagcttct ggatgctcct tagcagtaaa acgccactga acaaagttat tcggatgggt
    17761 ctctagtgca attggccggt gagcttccaa catatgaaca acttttgaac tttgtgcgtt
    17821 tgctaaaggc atcataggaa atcccatgga catcaaagaa gagataccaa tattgaatct
    17881 ggaatgcact ggagagatgt gaaaagcgat taatttcttt tccgaaattt ttgtgaaaac
    17941 tgttttttgc catttcgtcc gccatcccat agaatgagca gaatgacaaa taagtttagg
    18001 gataccagtt gtaccagaag tatgggtcat ataagaaatt tcatttttgt ctaaaaatga
    18061 ttgagaaaca gacaaagcag gttgttccat taaataaaga atagg
//
LOCUS       DACCQI010000065        17419 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCQI010000065 DACCQI010000000
VERSION     DACCQI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.066"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(332..1465)
                     /locus_tag="IVO76_001733"
     CDS             complement(332..1465)
                     /locus_tag="IVO76_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7429694.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(1456..2124)
                     /locus_tag="IVO76_001734"
     CDS             complement(1456..2124)
                     /locus_tag="IVO76_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7429695.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(2580..3566)
                     /gene="lacD"
                     /locus_tag="IVO76_001735"
     CDS             complement(2580..3566)
                     /gene="lacD"
                     /locus_tag="IVO76_001735"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7429696.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(3579..4505)
                     /gene="lacC"
                     /locus_tag="IVO76_001736"
     CDS             complement(3579..4505)
                     /gene="lacC"
                     /locus_tag="IVO76_001736"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP7429697.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(4518..5033)
                     /gene="lacB"
                     /locus_tag="IVO76_001737"
     CDS             complement(4518..5033)
                     /gene="lacB"
                     /locus_tag="IVO76_001737"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP7429698.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5052..5480)
                     /gene="lacA"
                     /locus_tag="IVO76_001738"
     CDS             complement(5052..5480)
                     /gene="lacA"
                     /locus_tag="IVO76_001738"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP7429699.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(5500..6273)
                     /locus_tag="IVO76_001739"
     CDS             complement(5500..6273)
                     /locus_tag="IVO76_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7429700.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            6438..6917
                     /locus_tag="IVO76_001740"
     CDS             6438..6917
                     /locus_tag="IVO76_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7429701.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            6974..7276
                     /locus_tag="IVO76_001741"
     CDS             6974..7276
                     /locus_tag="IVO76_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP7429702.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7298..8755
                     /locus_tag="IVO76_001742"
     CDS             7298..8755
                     /locus_tag="IVO76_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP7429703.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9348..10100)
                     /locus_tag="IVO76_001743"
     CDS             complement(9348..10100)
                     /locus_tag="IVO76_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7429704.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10315..10551)
                     /locus_tag="IVO76_001744"
                     /pseudo
     CDS             complement(10315..10551)
                     /locus_tag="IVO76_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(10561..11519)
                     /locus_tag="IVO76_001745"
                     /pseudo
     CDS             complement(10561..11519)
                     /locus_tag="IVO76_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(11725..13407)
                     /locus_tag="IVO76_001746"
     CDS             complement(11725..13407)
                     /locus_tag="IVO76_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7429705.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(13418..13741)
                     /locus_tag="IVO76_001747"
     CDS             complement(13418..13741)
                     /locus_tag="IVO76_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7429706.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(13764..15170)
                     /gene="lacG"
                     /locus_tag="IVO76_001748"
     CDS             complement(13764..15170)
                     /gene="lacG"
                     /locus_tag="IVO76_001748"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7429707.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            15637..16644
                     /locus_tag="IVO76_001749"
     CDS             15637..16644
                     /locus_tag="IVO76_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7429708.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(17038..>17419)
                     /locus_tag="IVO76_001750"
     CDS             complement(17038..>17419)
                     /locus_tag="IVO76_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP7429709.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         5226 a         3679 c         2861 g         5653 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgttacggt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaagagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acatttttga
      121 tcctttttat ttgattaatt atatccagct ggataataat tttgggggca acatttctat
      181 cttactatta tcttcctaaa gtagtagact ttcttattgg gaaaaattac aacaaaaagg
      241 agtggcttag ttgacaactc ctttttgttt acaaatcaat tggatatcta taaattgtat
      301 tttctaacga attttataga catctgattt gtcaaattaa gctagacaat atgtctttat
      361 ctatgcagct caaaaatact tccaatggtg ttcgataatt taatgatttt cttggaatat
      421 ggtttctttt cgaggcaacg gattgaataa aggtttcatc cgtgtcccta aagtccattg
      481 ttttaggtaa gccatcctgc ttgagcccat gcactcggta ctgatgagaa aaaattgatg
      541 cctgtttctc ctgaggaatg ccaattccct gatcttttac ttcaataatc acgctaccct
      601 tttggtttga aatggtgaca attaccagtt ttccttcacc atatttgatt gcattgtcaa
      661 ctaaattcga aaccgcccga taaatccaac gatctcgtcc atgaatcagg gttggttgat
      721 cttcagtaaa cgcaaacaaa agattcggat aattcatttt gtaggcatca caaacttccg
      781 cacagatcag tgaaacgtct acgacttccg tgctaagatc ctctgagtta ttggataatg
      841 tcaaaatatc gtcaatactg tcggtcatcc gatctaaaag cattaaattt ccttgatcgg
      901 catgttgccg ttctatttcc agattcgtac gcaaaatagc gatggcattt ttctgctcgt
      961 gggtcagata actatttaat cgtcgataat cctccaaatg accatcgaac ttatccttta
     1021 gcttattaaa agccgcattt aatgcctcgt ccttcacagt atttttagga acttgatcaa
     1081 taaacgttaa atcacgcgta atcgctaaga tttgattact gtaaattttg cgtaaaacca
     1141 accaaagaat aaacgttaat aacatgacgg cagaacaaat aaccagcacc atcgctggta
     1201 aatatttaaa tagttcgtcc tggtaagctt tatctatcat cgaaatagtc ataaattgat
     1261 tgttggaaga tttcgaagct tccatctgcg tctctgcttg aaccgtctta ccctcttcca
     1321 taaaataaac atcttcacct aaggtagatg cgatactgat tccctccaaa ttatttctca
     1381 gaaagaagaa actcactaaa atcacgcctg tcaccaacag tgaaaaagcc agcatcgtta
     1441 acgtcatttt aaaatttaat agcatagctg gtaccccttt gctcgaatgg tttttaagac
     1501 ctctttttca gctgcttcag ccagtttctt ttttagacga gccagatgca ctcgcagaac
     1561 cgacgaaaaa ggatcaaaat cttcatcgta gacatgttca gaaatttcct cagacgaaac
     1621 aaccgctgga tgacgctgag cgatgcacag taagatatca aattccttgg gtttcaggac
     1681 cacctctgtc ttttgatagc tgacccttcg acctaatgga tcaatcttca aagcaccaat
     1741 tgaaatctgc ggcagcgcac gtccatgata acgacgaata accgcgcgta ttcgtgcttt
     1801 taattctagt aactcaaagg gtttgactaa ataatcatct gctccaaaat ccaagccttt
     1861 tgctcgttct tcaatctcat cacgtgcagt aataatgata actggacttt cgacttcttc
     1921 agcacggaga ctttgtaaaa cagataatcc atctttttta ggcagattta aatctagtag
     1981 tatcccatcg tatgaattga tgaaggcctt ctcttctcct gactcgccat cataagctac
     2041 atctacagaa atattctcct gctctaggcc cttttttacg gataaaacca gctcacgatt
     2101 gtcctcaacc accagtacac gcatgtatat tcactcctca tacggattta gtaattacac
     2161 ttatgctttt tagcatagta cacgctcttc ttcaaagtcc acgttctttt tgtgaagatt
     2221 tgaagaaatt catacattct gttaaaaaac acataattaa gaatgatttt actgtttgaa
     2281 aatacctaga atagaaaaag actggaacct ttcctccagt ctcactactt tctatttgct
     2341 tggttttctt gtatatactt ttcaaagttc gttggtttat gaccaataag cctcgttaga
     2401 atactgaaaa ttgccatcaa gtcattaagt accacatgta agaaaatctc tctaaagaaa
     2461 aaccccttcg ttttaactca cttactattt tagaattcgt tcccctaata atgcagcatg
     2521 attttacgct ttatgtataa aaaaagactg caagcaattt tgcttcacag tctgatttct
     2581 tatgatagtg agcttgttaa cacttccacc ggtgtagcag tttctttcaa tacggcattt
     2641 aattcttcaa tatttttacg cccttgcgta ttcatccact ccatcgctgc ttcttttcca
     2701 tcaacgatat agctttctac accatctgcc caagtcgcac ggccgcaaag tacaccattg
     2761 aaggttgagc cggcattttt cgcgaagcgc agtgtttctt ggaacaactc tgcttttaca
     2821 ccggcgctta ggaaaataaa tggcaggtta gtggcttcag cctgttcctt gaagaagtta
     2881 gccgcttgtg cctttgtgta cacgacttcg tctgtcccat agccttccac ataattcata
     2941 ttgacgggta cttccacctt taacacatct acgccataac gtggatcaga aaattccttc
     3001 atcatatcgt tgactttatg aggtttctta atggcgtatt ctttcgaagt cgcatcagag
     3061 atgttcgcgt cataagaaac aagctccaag aagaagggta agttttcagc ctgacattct
     3121 gagccgattc gttcaataaa ggcatgtttt tgattattga tttcttctgc ttcatcgaca
     3181 tcatagtaaa gtaggaactt acaagcatcg gcgcctgctt cttttaaacg tttaactgac
     3241 cagatcgata ataaatctgg taaacggccg ggagttgacg catcgtaacc agttttttca
     3301 taagctaaca gcaagccggc attttttgct ctaacgtcag ctgctggcaa gccatattcg
     3361 ggatctaata aaatcgatga ggcatatttc gttaattgtg aagaaacaat ttctttaaat
     3421 tcaacgattt ctgattcttc tgcttcgctg cccttgaatt ttgtgatcat cttcttcaaa
     3481 gccccgcgtt gatcaattgc taaggcccca atcactccat cttcattgca tagttgcttg
     3541 atatattgac gtttttcttc gttcatttcc gtatcctcct acactttctc cactaagatt
     3601 tgatcatata gtttttgata attggctaat tggacatagc cagtcgtcgc ttcctgagca
     3661 ttcagcattc ctaaaacatt accctgtttt aacacggctt catccgaaag accttgttga
     3721 atcgcagcgg tcaatccggc aacggtcgca tcaccagagc caactggatt gacgacatca
     3781 atctttggaa ttgtcacgca ataaaaagtc tcgtgatgtt tggcaaaggc tccgtcgccc
     3841 cccattgaaa ccactatcca ttcaatttca tcaaacatcg aatctgacaa ggcggctttt
     3901 aaatctgata attctgttga gatttctcgt cctagcagac cagccagctc ttcggtattg
     3961 ggttttatta gcttaggctt tacactgcct cgcaacactt cccttaatgc tgtacctgaa
     4021 caatctaaaa cgacaggctt tggctgacaa acttgcagca tttgtcgata aaaatcagca
     4081 ggcaatcctt ctggcaagct tcctgaaaaa gaaatgattt cggctttaac agctaatttt
     4141 tcgaaatgtt gcaagaacgc ccttgcttct tgttcagtaa taacaggtcc gttttctagg
     4201 atttccgtct gttttccgtc atgtaaaaca gcgatgcaat tacgtgtctc tccgctgata
     4261 ggtaaaaagt catgagcaat ctcattttga gtcaggtcct ttttaatctc ttcacctaaa
     4321 aaaccgccga ttaaaccact agcaatcacc tccgtatcta attgcttcaa gatacgtgtc
     4381 acatttaggc ccttaccgcc agctgttttg cgaacatttc ttactcgatt gactgtgtcg
     4441 agcttgaagg tttctaatgg ataagaaata tcaatcgatg gattcatcgt tattgttaaa
     4501 atcatttgtc cacccgctta atcgtggtat tcgccacgat cccatttctc gataaattca
     4561 tcgaagaaat gcggatcaga ctgcttgtca ctggcttttt cgacgtgatc gattttggcg
     4621 atcaattttt tgttttcttc agtttcttga taatcggcat tgatgaaggc ctctaatatg
     4681 tcacagatca aaaattctcc cgtaatccga gcgccaaagc cgatcacatt ggcatttagt
     4741 tgttccttcg catagatagc agtcgtcata tcgcgaacca gcgcactgcg gataccaggt
     4801 actttattga cagcgttgtt aatcccaaca cctgttccac acaaacaaac tcctagatct
     4861 gcttctccgc tagtcacggc ctctccgact ttttttccgt agatcggata atgggttctg
     4921 gtaaagtcat aggtccccat atccaacact tcatgtcctt ttgattttaa gaaatctgat
     4981 actgccattt ttgtatctgt gacgatgtgg tcacagccaa ttgcgatttt catttcattc
     5041 atccttccta attacgccat tttatttaac atgtcgacac gtacttgatg acgaccacca
     5101 tcataatttg cttgtagaaa ttccttaaca atattcaacg ccaactcatc gccaacaatt
     5161 tttgatccaa gtgtgatcat ccgagaatta ttatgtttac gggtcatata cgcactgcgc
     5221 tcatccgaaa cttctgctgc taccattcct ttaacctttg ttgccgtgat aaagctgcca
     5281 gcgccgtagc catcaaccgc gattcctaaa ctaccttcgt tttcattcag atcatttgcg
     5341 atcgccaaag ttgattccac aaaatcttct gaagccgttt cactcttatc tactactgta
     5401 ataccttctt tgatcaattt tttcttgatt aattccttta attccatgcc gtcactgtct
     5461 gaaccaatta ctacacgcat ttttgtttcc tcctattttt tactgaatga ttttagtgaa
     5521 ttgctgataa taactcttca gctcggctga aagagtagaa tccgtaaata atttgtcgat
     5581 cttgcgcaaa tcatagaaat tataaaaatc gctatggtcg aatttgtttg catctgaaac
     5641 caaacatttt tctgccgcat tatctaaaac aattttttgc aaggtacctt cttcaatact
     5701 gaaattcgaa acctccgaac ccttcaatcc attcactccg acaaaagcct tcttaatacc
     5761 gattttttga acagcttcat tggcaattga accgacaaag gctccagtct tttcccgata
     5821 tagcccaccg atcaaataca gttcaaatct ttttatttca cttaatagat tgaaaaccgg
     5881 cagactattg gtaacgattc ggagagatga cggcttgatg tatcgtgtca ttaattcgat
     5941 cgttgtaccc gatccaagaa aaatcgtgtc gtcttcttga ttgatatgag ctgccgcatt
     6001 tttagcgatg tattccttct cttttgtatg cagcttcttt ttttcagtat gagaaagctc
     6061 atttgcttct atagaagtcg acatttccag ataatcgatt ttttgtgcac cgccatgaac
     6121 gcgtaaaagc tttttcttct gcgcgagttc ctctagatct cggcggatcg tcatatctga
     6181 aacattcagt acttcctgaa taaatttgac tgttatcgtc ccatagcgat cacacatcgc
     6241 taagatcgaa cgctgtcgtt ctgttttcag catattctgt acctccacga ccttataata
     6301 aaacatttac gaacattttt caacacatat tgttcgttta ttttcgttta tgcgttgaca
     6361 atgattttgt aagcggttat actcaacttg taaacatata aataaacata aacgaactaa
     6421 taaaggagct gttttgtgtg aatgaattct ttttcaaaga cactgtctat gtttctgaca
     6481 agcagtcgca ggaagaagtg ttcgaggaag tctatcaaga tctgttaaag aaagaattgg
     6541 taaccgaaga ttttttggag aaccttttag aacgggagcg taattatccg acgggattag
     6601 atctctcacc tgtctctaaa atattgccag atatcgctat ccctcatacc gaaagtgagt
     6661 ttgtgcgaac cacccgtatc attccaatca aagtaaaaaa tgaaatccct ttctacaaca
     6721 tgatctcgcc aagcgatcag atttccgctt catttctttt catgatcttg aacgaaaatg
     6781 gtgaagcaca ggctggcatg ttggctaata tcatggattt cattaattct gttgacgaag
     6841 aaaaactgtt ttcgtttttc aaattagagg acacagaagc gatttatcat tttttagaag
     6901 aacattttta tatatagaag tctctgccta accgcaggat aaaaaataaa cttattttaa
     6961 ggggagaatt attatgatca aaattttagc agcatgtgga gcaggcgtta attcaagcca
     7021 tcaaatcaaa agcgctttgg aaactgaatt gaaaaatcgt ggctatcaag tcacctgtga
     7081 cgccgttatg atcaaagata tcaatgaaga aatgctgtct cattatgaca ttttcgcaca
     7141 aattgccaac accgacttgg gttttgacat taacatccct gtcgttgatg caggtgctat
     7201 tctttatcgt attcctgcta tggctgagcc tgtttaccaa agtatggaaa gcgcaatcca
     7261 atcattaaac aaataggact aaagaaagga aaagcatatg aatacaatta ttgatattgc
     7321 caataaagta tttacccccc taattaattt aggtgcggca ccgttaatga ccatcgtatt
     7381 aactgttatt gctttatttt ttaaagtaaa accgtcacgc gctcttgaag gcgggctcaa
     7441 attagggatc gccctgactg ggatcggagc tattattgga attttaacta ctgctttttc
     7501 agacgctatg acagcttttg ttgaacgcac aggtatttca ttaaacatta cagatgtcgg
     7561 ttgggcccca ctagcaacca tcacatgggg gtcgccatac acgctttatt ttttactaat
     7621 tatggtgatc gtcaatattg tcatgttagc aatgaaaaaa accaacacat tagatgtgga
     7681 tattttcgat atttggcatt tagcaattgt tggtttgttc gcgatcttct gcggtgctaa
     7741 tctgtttatt gcttctgcat tagttatttt cattggtgta ttaaaaattt ggaactccga
     7801 cttaatgaaa ccaaccttca atgacttgtt aaatgcgcca gacagcaacc cgatgaccac
     7861 gactcatatg aactacatga tgaatccgat tattatggtt ttcgataaaa ttttcgacaa
     7921 aatcttccct tggttggata aatatgattt cgatgctgcc aaattgaata gtaaaattgg
     7981 cttctgggga tcaaaattcg cgattggaat ttatttgggg atttttgttg gattactagc
     8041 aggacaaaca ccaacgcaaa ttttctcgct agcctttacc gctgccgtct gtttggaatt
     8101 gttctctttg ataggcgctt ggttcatcgc agccgtagaa ccgttatcac aagggattac
     8161 cgattttgcc aataaacgac taaaaggacg taccatcaac ataggtttag actggccttt
     8221 catagcaggc cgcgctgaaa tctgggctgc cgctaacgta ttagcaccaa tcatgttgct
     8281 agaagccatc atcttaccag gaaataaatt attgccacta ggcggaatca tcgcaatggg
     8341 tgttacccca gcactattgg tcgtaactcg tggaaaatta atccgtatga tcgtaatcgg
     8401 cgcgatcgaa ttaccattat tcttatggtc cggcaccttg atcgccccgt tcgttactca
     8461 aacagctaaa gcagtcggag cctttccagc cggcttgaac gggtccgcca tgatctctca
     8521 tacaacgatg gaaggcccga ttgaaaaatt cctaggttat ttagtcggta atgcttctca
     8581 aggacaaatc gaatttgtga tatacgctgc cttagcatta ggtgcatacc ttttaatctt
     8641 catttggtac gctcgtcaaa tgaaaaaacg caacgctgca tacgcagctg aaaaagaaga
     8701 gaatgcatct ccggcaacgg cgcataatat gacttatgca gccgaagcta aataagctaa
     8761 aaacaccccg aatgtcttta gacttcgggg tattatttta cctataaaaa ataaatagat
     8821 gtaaaatcca tttcaaaaag catagcccat ctcttgccac acccaataaa gaaattatca
     8881 acgtttatgc tttttttaac tcctttaagc ttgcctttgt tggtattcct caacgattct
     8941 ttttgtgttc ttgtttgttt tgtgcttttt gactttttgt tgtttaattg cgaattcctt
     9001 gatttctttt ttcctgaatc gcttcgcatc ccgcggttat gcgtgcgatt tcatcattgc
     9061 tttttctgat ctaactcgtt tcatgtcaaa gagcttcagc tgacttagtg tccatggttg
     9121 ttttgatatc ccttttcctc cttaataaag cggtgattga tactgctatt ttttccttca
     9181 cataaacata acatcccaaa aaaatcgact atcttttttc aaatggtgta ttcaagtatt
     9241 cggaggaatt agtaatgatg caccctaaaa ataacataat tcgcttaaat tcaaggctac
     9301 caaaaaacta ccaagttcta attatctaga gtttggcagt ttctcattta cacaacatat
     9361 ttttcttctt tttcgatttt ttcatagtgc ttttgttcat catcatccgt tataactact
     9421 gttacatcat tcagattata atatactgaa aaatctcttt tcccaatttt cgaatgatca
     9481 atcaataaaa agcgttcaac agaattattc agagcgatct gttgagtttg gccctcttca
     9541 aaagtcgaag tcataatttt ctgttcattt aaagcattac agctgaaaaa tgctttatga
     9601 aaattcatat cctgtagcga tttattcgtg atttcaccaa caaagcattg agtcgtttcc
     9661 cgcatctcgc cacctaatag atagattttt cgattccctt ttcgcttatt taattcttca
     9721 aaaacaggca aacagtttgt aacaatccgc agtgaatccc cttgaatgat ttttgacatg
     9781 agctcgattg tagttcctgg tccaagaaat atcgtatcac catcattgat catttcattc
     9841 gccttttcca caatccgctg cttttcttct tgcagcataa tctgtttttc tctatgagaa
     9901 agctccacat gccgataaaa atccttcaat ttcgctccgc catggacgcg ttctaataat
     9961 ccttgtttct ccatttcgtc caaatcacgt cggactgtca tatccgaaac cgaaaacttc
    10021 tccataatat cgctgactcg tactgtaccg ttaacctcta ataacgacat gatcgcttgg
    10081 aatcgatccg tttttaacat ttcatcgcct cttttctttt taagactatc aaattcttaa
    10141 gaaaagtgct agaacaagtt ttcttaagaa gccttctgca ggaaggaaag caaaattttt
    10201 cagaatttgc aagctgctat tctaaaaaat aaaaccgaac gagcatgact gtcatagtga
    10261 cagccttgtt cattcgactt ttatctttaa taaatcattt tatttaatct gaagttattg
    10321 tgcaagttca aaaacaaagt agttaatgtc tcctcgatat ttcgaaatcg aatactcaac
    10381 gggcagacgt tctagcgaaa aaccctgtgt atggaaatag aacattggat ctccttcttc
    10441 cacatctaac aaatcgctga tcgtatcatc cgctttgatt acttctagtt ttctggtgac
    10501 cgttttaatt ggataaccca tttcttcaaa aacttgatac attttttctt ttctgatttg
    10561 tcaaattaag ctagacaata tgtctttatc tatgcagctc aaaaatactt ccaatggtgt
    10621 tcgataattt aatgattttc ttggaatatg gtttcttttc gaggcaacgg attgaataaa
    10681 ggtttcatcc atgtccctaa agtccattgt tttaggtaag ccatctcgtc taagcaagcc
    10741 gtttgagtgc tcatttagcc ctctttgcga aggtgttcct ggatctgcga aaaagatggc
    10801 aatatcgttc gagttactta tagatcgcca gttggaaaat tcttttccac aatcaaatgt
    10861 catggattta aacaagtgtt taggcatgga tttgaaccaa tcatttaacc tgttttcgat
    10921 atcttcggct cgtctgcctg tcggtttcaa agtaataata accttggata aacactcaac
    10981 caatgtaata acagcgctct tgtgtttaac tcccacaatg gtgtcgcctt cgagatgacc
    11041 aaattcttta gtcaggtgtg gatagagact gttccgttca tgaatcgttc tgcgaaaggc
    11101 ctgttttcca cgcttttctt gatgattatt cggtttacgt ttccccttca taggtaacga
    11161 ctgtgtgtca aaaaggcctt ttttaaacat ccgatagagt gtacgaacag aacaatcaat
    11221 tggaaactcg ccacggccga taatcacatc gggtgaccac ccttgagcta ccttttcttg
    11281 aacatatgtt gattgttcat cagataaaac ggttgaacgt cgtccacaac gagatttatt
    11341 ttgtttgtat tgacggtaat aatctagcac ttttttcctt gtttgagaaa cgaataaaca
    11401 ttataaatgg tttgtctaga gcgatttaaa ctttgtgcga caagagcgac aggtttctcg
    11461 atattgaaat aagactctat gattactagt tcgtctgttg taagatgggt gtaggtcatt
    11521 tgtactcact ccttattaat tctttggtcg gaactatttt gagtgtagca caaatgatct
    11581 ttttagttgt ctagcttaat tttacaatcg acgcctcaaa aagaataccc gaaatttaaa
    11641 cagcaaaaaa agatagcgct taccattgtg tttaagataa aacgctagct aagttggcta
    11701 gcgctttatt aatctaagca agctctaaaa ttgcttcaat acaaaatcaa ctgctccttt
    11761 aggatcacgt gttaaattga tatattctgc acctttagtt gcaattactt ttatccctaa
    11821 tttgtctgca tctttacgga gatcatccaa ataggaattc acttgaggtg ctagaacgat
    11881 taaatcaaaa tttttcatca tatcataatg agaaccataa gctcctgctt tagcagaaag
    11941 attaacacct gtctcttttg cgccctcttc taatgcatta gccaacattg cactcgttcc
    12001 ggcaccagca caaagtacta aaacgcgctt tccttcacca accgatgctt ttgcttcatc
    12061 aactataact gccgcagttg caccaacttc ttggatttct tcgaccaatt cttcttttac
    12121 ttcttctgca gcaatgtttt ttaactcttg ttctactaat tcagaatcat aggccttaac
    12181 aaaaggtaaa taaataatcg catcgacaac aatcaatact accgctaata caatcgccaa
    12241 cacactaaac ccggttccta aaaccaagcc aatcggtgcg ggcgtcgccc aaggcaatac
    12301 ggatataaag ctattcatcc ccaagacatc gacaaaaagt ttgaatatcc atacgttagc
    12361 gattggtgct aataaaaatg gaattagaaa gtacggattc aaaataatag gagtagcaaa
    12421 tagtaacggt tcatttaccg caaacgaaac tggaataaat gaagctttac caactgcttt
    12481 caattgtttt gatttagcga acaacatgaa cagaaaagga acaactagtg tcgctccagt
    12541 accacccatc gttccaacaa agtttcctag accaactgtt aatacttttg aggcttgtgc
    12601 accgttttta aataattcaa agttagtttc tacgttcgca taaataattg cagcaatcgc
    12661 tggttcaaca atcgatggtc catgaacacc aacaaaccag aacattgcca tggctcccca
    12721 aatgattgca ataccgacat atccatcagc agcactaaat aatggctgca ataattgaat
    12781 tacacctgct gcaaatgatg ttccaaaaaa ttgacgaact cccgtatcaa tcaaaacggc
    12841 aataaatact gcaattgaaa aggggataat atctctaaac atctgtgtga ttgttccggg
    12901 aacttctttt ggcatacgga tcgtcaaatc tcttttaaca cagaatttat acacatttac
    12961 agtaacaaac gcagaaacga aagctgctaa taatcccttt gtccctaaat aatcagtagc
    13021 tatcccacca tcaatttgat cagctgccat cattacaaaa ccacaaattg acgctaacat
    13081 aacagaaact tcattaatct gcttacctga atcagataat tgacggttca ttgaattagc
    13141 taaacatcga gctgttgtag caacaaccaa aagaccaact aatcccattg aataaccata
    13201 aactttccaa agccacgtcg agaatgcctc tggtaacgtc acgttaaata cttctggaac
    13261 agccgcaatc ataataaaaa tacttgaaaa tagaatagct ggcatagcag ttaaaaagcc
    13321 atcgcgaatt gcccctaaat aaatattacg tgagattttt tcaaaaaacg gttttccctt
    13381 ctcaatttgc ttaataatcg tattcatttt tcttctccta tgctttagca aatttttctt
    13441 tgtacaattc aacaaaatca ttgataagtt ctcttaatag aatcgttgtc attaaatggt
    13501 cttgcccatg aataaaaatt acgcctactt ccatttcgtt ccctgacgct tcttcagcta
    13561 aacatttcgt ttgtgagtta tgcgctaagg ttaaattttc atcagcatcc gctaataatc
    13621 gttcaacatt atcaaaatta ccttctctca cttccttcaa cactgttaat aatgaacttc
    13681 tggcatctcc tgcataagca acaatttcaa atcctaataa ttgcaattct ttcctatcca
    13741 tttccttgcc ctcctaacta tttttatatt atcgtttttg tttcgctaac aaatttatac
    13801 caatatgcag actctttcgg atatcttttt tgtgtatcga aatctacata gaatagtcca
    13861 taacgcttag tgtatccatt aacccaagag aaaacatcca ttaatgacca aatgaaatac
    13921 ccctttacat taactcccat tttgatagca tcggaaatcg cattaaaatg ctggcgtaaa
    13981 taatcgattc gatcatcatc tagaataatc ccatcttcaa aattatcctt ataccctagc
    14041 ccgttttcag taatgtatat tttattataa tttggatatt cgtcttggat tcgtacaagt
    14101 aaatcatata acccttttgg ataaatgatc caatcccaat cggttttcgg aagatcctca
    14161 cgcacgattc tctcgccaat ccctttaatg caatagactt ggctaccttt ttctccggta
    14221 gtgttgtagt gaagtgtgct ttctcctttg tactctttaa cccaatggct accatagtta
    14281 ttgataccaa gaaaatcatt tcgatttgct gcttttttca tctctctaaa atcattagaa
    14341 tcaatatctg cagattgccc atttgctgct aaaatctcat taatatgtgc cattgtttca
    14401 tctgagtaat accctaaata agtagcatct aataagaact taattgataa cgcatcatct
    14461 aatttcgcag catgcttatc ttcttctttg tcacttgctg gatattttgt ctctagcgag
    14521 tgaacaacac caatttcccc tttatatcca ccatctttaa aaatgtttac tgtctttgcg
    14581 tgcgcataca tcatgttgtg taaacaagtt acagtcttct ctatatcaaa ttgaacttct
    14641 ggaggaaacg atccgatcaa atattgattt gaagcaactg gataaatctc attaaatgta
    14701 ctccaataat gaacctcaga aaattcatcg aaacagaact gtgcatagtt tacgaaatgt
    14761 tcaacatttt ctcgatttaa aaaatctcca ttatcaaata gttctttagg ggtatcaaaa
    14821 tgatgaagag ttacaaaagg aataatacca tgcttattca attcagcaaa taccttatga
    14881 taatattcca ctcctgtttg gttcacttcc ccatagcctt ttggaaaaat acggctccat
    14941 gcaatagaca tcctaatact atcaatttta aattttttgc ataattcagc atcaactgga
    15001 tattgattat aaaaatcact agctggatca gcagtatagc gtcccttttc tacaaggaaa
    15061 gtatcccacg ctacttgccc ttttccacct tcttttgtag agccttctgc ttgataagct
    15121 gccgtagctc ctcccataat aaagtcttct ggtaaagttt tataattcat tttaatctct
    15181 ccttgaaaat tagtttgatc aatatgccaa taatccataa atcacctcag cataatttta
    15241 acatgtataa gtaaattttt ttacacaacg aaaaaccgac ctcgaatatc tattttattg
    15301 cgatatacaa gcacttatat cccttgatgt aaaataaata taccattcaa aaaccgtttt
    15361 gtaaacgttt ttttgtaaaa taaatttatc tttttactaa tttaatttac acgaaacaag
    15421 aaaacggact gattcccact aaaatcaatc ccacctacta atcattctcc aagtggctat
    15481 ccattatcga ttcaatcttc tctgttaatg gattaggctc tctgctgata tcagagtttc
    15541 gtttcctgct agaaaaagta atttaagtta ctaaaagagc agtttcgatt tactttaaca
    15601 aagaaaagga ttattatatc cctaggagga tgaacaatgg ttactattag agatattgca
    15661 aaaaaatctg gttactcaac ggctactata tcaagacttt ttaaaggtga tgagtcactc
    15721 tctattacat ctgatactaa aaacaaaata atcgttacag ccaaatcttt aggatatgat
    15781 ctgacaaaaa ttaagactac tctgtacaag attgccattc ttttttggat ctcagaggaa
    15841 cagttaaaaa aggatgtcta ctatcaacat gtcaaagatg atttgaaaaa ttatagtaaa
    15901 aaagctaatc tagatttgat tttcatctct aaaaacgatg gtgtaaaaag cattcctgac
    15961 gatattactg gatttatcgc aataggacaa ttgacaaaaa aggaattgta ctacttaaat
    16021 caaaaagggt ataagggggt aatccgagga ataaacccat tacctgaaca cttcgatact
    16081 gtagaacctg atttccacga aatggccaga caagcaatta actactttat taatgaaggt
    16141 ttcacaaaaa ttggatttat tggaggaaat ttctttaacc tagaaacggg taaagaagag
    16201 attgatagcc gtgaatatgc cttccgatat catttagaaa agagcggact actcaatgaa
    16261 aagtatattt tctcccaagg atcatttact gttgacgaag ggtatcaatt aactcttaaa
    16321 atgaaagctg agttaaatga agatatgccc gaggcatgtt tcataccatc tgacactata
    16381 gctgccggcg ttcttcaggg ctgccatgaa ttgggtatta acatccctaa agatatggca
    16441 gtaataagta ttaatgatga cgacatcgca aaattcgttg caccaccact tacaactttt
    16501 aagatagata tcgaagagat agctaaaacc tgtgtagatt tattaagtga tcaactggta
    16561 tattcgcggg acatatcaaa gaaaacatta atcggcgcaa aattgaccgt taggaaaagt
    16621 tttatcccta aagaagaggt gtaaaataag taattgaaac ttatctttaa aactataatt
    16681 gtgtccacat agtagccgag caatcgggtt tatgcctatt atgttttcca taaataattt
    16741 gatggtgata ttttgacgta aatacaatat gattttttca tcgccaaatt gtgcgcgcta
    16801 atttttgttt atcattcaga acatttcctc ctttttatct aagtatgagg ttggataaac
    16861 gcttcatctt agtatcttgg gggatttttt atctcgttgg gaatatatca agcatcagag
    16921 cgcgcaaaaa gaactggtcg aaaaatcaat ctaccagttc aatctcattc actgtgatac
    16981 tatgttttat acagataacg ccatattata aaacaagttg tattccgcaa atgggttcta
    17041 ttcaactctt aaactatcta taaaagattc agccaactcc attagcctct cgtaatttag
    17101 ttggtgaaca gcactttttt caaaaaattt tgaaccaatt cctacataag aggctccatt
    17161 gttcaaatag ttttgtgcat tagtctgatc tacccctcct acagccatca aaggtaaact
    17221 tcctaaaggg gcttgcactt gattgaaata ttccggtcca agacagttgg cggggaacac
    17281 cttaacaata tcagcaccca tctcaaacat tttgaatatc tctgtgggcg acatggcccc
    17341 aggaattgtt agaaggttct gttgcgaagt ttgaaaatca aacgcgccct ctctgaactc
    17401 caaatatctt aggctgtta
//
LOCUS       DACCQI010000066        17007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCQI010000066 DACCQI010000000
VERSION     DACCQI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.067"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..126)
                     /locus_tag="IVO76_001751"
     CDS             complement(<1..126)
                     /locus_tag="IVO76_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP7429710.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="IVO76_001752"
     CDS             complement(230..541)
                     /locus_tag="IVO76_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7429711.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="IVO76_001753"
     CDS             complement(543..740)
                     /locus_tag="IVO76_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP7429712.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="IVO76_001754"
     CDS             complement(1134..2861)
                     /locus_tag="IVO76_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7429713.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="IVO76_001755"
     CDS             complement(2854..4047)
                     /locus_tag="IVO76_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7429714.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="IVO76_001756"
     CDS             complement(4234..4878)
                     /locus_tag="IVO76_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7429715.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="IVO76_001757"
     CDS             complement(4972..5106)
                     /locus_tag="IVO76_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7429716.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="IVO76_001758"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="IVO76_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7429717.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="IVO76_001759"
     CDS             complement(7237..7710)
                     /locus_tag="IVO76_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7429718.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="IVO76_001760"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="IVO76_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7429719.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="IVO76_001761"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="IVO76_001761"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7429720.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="IVO76_001762"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="IVO76_001762"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7429721.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="IVO76_001763"
     CDS             complement(11757..12896)
                     /locus_tag="IVO76_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7429722.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="IVO76_001764"
     CDS             complement(13205..13918)
                     /locus_tag="IVO76_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7429723.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="IVO76_001765"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="IVO76_001765"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7429724.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="IVO76_001766"
     CDS             15107..16639
                     /locus_tag="IVO76_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7429725.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="IVO76_001767"
     CDS             complement(16830..>17007)
                     /locus_tag="IVO76_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7429726.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DACCQI010000067        16697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCQI010000067 DACCQI010000000
VERSION     DACCQI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.068"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(146..1111)
                     /locus_tag="IVO76_001768"
     CDS             complement(146..1111)
                     /locus_tag="IVO76_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7429727.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="IVO76_001769"
     CDS             1345..2190
                     /locus_tag="IVO76_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7429728.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="IVO76_001770"
     CDS             2381..3568
                     /locus_tag="IVO76_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7429729.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="IVO76_001771"
     CDS             3574..3930
                     /locus_tag="IVO76_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7429730.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="IVO76_001772"
     CDS             3932..4270
                     /locus_tag="IVO76_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7429731.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            4665..5699
                     /locus_tag="IVO76_001773"
     CDS             4665..5699
                     /locus_tag="IVO76_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7429732.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="IVO76_001774"
     CDS             5692..6378
                     /locus_tag="IVO76_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7429733.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="IVO76_001775"
     CDS             6402..7250
                     /locus_tag="IVO76_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7429734.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="IVO76_001776"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="IVO76_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7429735.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="IVO76_001777"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="IVO76_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7429736.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="IVO76_001778"
     CDS             9516..10751
                     /locus_tag="IVO76_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7429737.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /locus_tag="IVO76_001779"
     CDS             10738..11208
                     /locus_tag="IVO76_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7429738.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="IVO76_001780"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="IVO76_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7429739.1"
                     /translation="MGVPELEEYEFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(12699..13841)
                     /locus_tag="IVO76_001781"
     CDS             complement(12699..13841)
                     /locus_tag="IVO76_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7429740.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13880..14614)
                     /locus_tag="IVO76_001782"
     CDS             complement(13880..14614)
                     /locus_tag="IVO76_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7429741.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            14847..15122
                     /locus_tag="IVO76_001783"
     CDS             14847..15122
                     /locus_tag="IVO76_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7429742.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            15220..>16697
                     /locus_tag="IVO76_001784"
     CDS             15220..>16697
                     /locus_tag="IVO76_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429743.1"
                     /translation="METLMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPL"
BASE COUNT         5488 a         2962 c         3500 g         4747 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatatg aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atatataagg gttctagctt ttttgttttg
    12661 cctgtatact agaaatgacc agattttgac cagctctttt actgatgagc catcatatca
    12721 gcaaagtctt gacctgtttt cttaggtgca ttatttgtaa tatgtgcata gatgtctaat
    12781 gtcactttac tattttcatg ccctaatcgt tgttgaacta ccttgacaga ttctcctgct
    12841 tctaataata aactagcatg tgtatgtcta aacccatgag gtgtaatacg aggcaagtta
    12901 tattttttca aaatatatct taaccaatca ttaacagtgt ttggaatgta tagagtgttt
    12961 ttaactgttg taaaaacatg ttgctcttta gagctagtat tgtatccaag ttttaaatac
    13021 tcttcttttt gctgtaattt ccagttgttg aggatagaaa gagtctcagt atctaaagag
    13081 ataactcgtc tagaagctct tgttttcgga gattgaatta taactttacc aaattcatct
    13141 acagcaagag ttttattgac atgtagttct ttattaaacg tgtctatgtc tttccactgt
    13201 aaagcgagta cttcactctt tctcatacct gtgtatgcca ataatctgaa gaaggcgtat
    13261 tgtttcatgt taccaaaatc tttaaaagca tccagtaaat tattaagctc ctcttttgag
    13321 taaaatttac gatttttctc atactctttt tttcgtggaa gaagtgtttt ttccatagga
    13381 ttattgtcaa tatattgcat tgcaaccccg tagcgtaaaa tttgtgcagt ctgttttctc
    13441 atataatcgt aactttcata tttactgtgc cattcatcta ccactttttg acaatatggg
    13501 acagagatat tcgatagttt aagcttacca aatgctggaa gtatttgatt atttgcgtat
    13561 cttactgaga ttgcgattgt tgaagcttta acactggtac gctgatgagt tatccattca
    13621 ttatacaggt cttcaaatgt catatcaggt gctttctttt taacaccgta taatatttga
    13681 tgtctgtact cgtcataatc tttttgagct tcttgtttgc tactaaaacc tcttctcgta
    13741 gtatttattt tttcaccagt tcgtggatcg gtggcaatgt gtcctttata catccacgcc
    13801 acttgaccgt ttttcttttt gtactttgat atttttgcca ttaaatcatc tcctcttagt
    13861 ttacaaggag tacttaccac tagatggtat cacctccctt catttttttc ttagtgtatt
    13921 ttttcccaat tgatgaaatc gtttgttcta gcttgtttat attatcagta atttctctca
    13981 cagcttcttc gggataagac atgtctttca attgaatagg tagccattca tctaccattc
    14041 tttctagtag ttcttctata ctgctagtga ggaaaagaat gttattgtca aattcttcta
    14101 caatagcatt gatgaataat tcttctattt ttaagatatt gaaaagacta attttttcat
    14161 ttttaacttt attacaagtc ttgttagcaa taaattcttg catctctggt tctaatttgg
    14221 aatatctatc cagccatgta tctaaattag agtgtactgg tgggaactgt tgacgaagat
    14281 gctggaacag gtttttatct ggaaatgtta taccatctag tacaatgcta tctctataaa
    14341 gaataattcc caaaatatat tcttctacat tgccacaaac taactcctct atactcatat
    14401 ttcccaaatc agcaattcgg gataatcgct ctggtattgg taaataggtt cccttctccc
    14461 attcgtagat agtttttttg tcaattaatt gaccgttgtt aatcataaga ccaaatgctg
    14521 gttgtgttaa ttttttactc tttcttatag cttttatttt tgttccgagt atcttttttc
    14581 gttctaaata atttattggt ggacgtttta tcatattgac cacacctcct aaaattagta
    14641 tacctcaaaa aaaggggtgt tgtaaataaa ttttacaaag atgttgacac ccctttttta
    14701 tcgaggtatt atgtgtatgt cgatgaactg gtaatcattt tttgtttgat taatcggtta
    14761 ataacaaaat acttttattt tgagcaaaaa atacgaagat aattcttaga aaatttgttc
    14821 aagcaatcac gaaaggaggg ggaaatatgt ctctacaaat cattgatagc attgtttcta
    14881 tacaagttga acaacaagtg aaagagataa ttacagtgct aaagcctatg ctagaagaca
    14941 ttatttctgg agtcactcta gagtatatgg actatcagca ggcaagtgaa tatctaggtg
    15001 tatcagtagg aacgattaga aaatatgttt ctcaatacgg gttacctgtt ataaaaattg
    15061 atacggtcat tagatttaaa aaatctgata ttgatagttt catggaacgg tataaaggtt
    15121 gagtaaacaa ataagactaa aacatatagt aataaagagt ttgtttttat tatctgtatg
    15181 ttttagtctt taacagaatt ttataaggag gaaacacaaa tggaaacatt aatgattaat
    15241 attccaattt atgtagatca agaaaaatta ttagagaagc cagttacaaa aaaagaaaaa
    15301 actgatgtaa gagataggtg tttagataac tatagagaag ttacaagaga tgagtttgta
    15361 gagatactaa attcatcaca atcatttatt ccaagcaaga caaagaataa aggcaatgac
    15421 aaggagagtt ttgtggaaac cagagtaatt attctggatg tcgataacac tgttaaagat
    15481 gagaagggga aggtagtaga tttgagtaaa gatgatagta gatacttaag cgtagaaaag
    15541 gttttatcta tcaatttagt tcataattct gcgtttgcga tacaaaaaag tattcggtac
    15601 tctgaaaatc tagaaaaata caagattgtc tttctattaa aagaagcaat tactgactat
    15661 ggtaaaatgg ttgctgttta cgaatacttg aaaaagaaca tccccgggtg tgatgacaat
    15721 gtaaatgctt cgaatagaat gttttttgga ggatataagt cagatgcagt gataataatt
    15781 aatgaagaca acatgttaga tattactgag cttccgattg attttaccat gacatgttct
    15841 aaaaattgtc aaagtgattc agggagagag tatgaactag tcaatgaaac cgattttgtt
    15901 aagttgataa aaaatgacga caaagaagaa atgaaacagt ggtttaaaga ctgcctgttt
    15961 gattcctccg acatgtcttt taatgagatt tatgaaagat tgttaaccat tgatatgaat
    16021 aaattactta agtcaagtaa aaacttaaga tgtctgtttc acaacgatca taatccctct
    16081 gctagtattt ttaccagtaa aactgggcat tccatttttt attgtcattc cagtaattgt
    16141 gatatcagtg aaaattttat tggagttgtg atgctaattt taaagaaaga cagtcgggta
    16201 gaaacattta attggctatt agagaaccta gacttgtatc ctacacattt tcgaaaattg
    16261 aaagaggaat caaaagtact ttttgacact cttgaatcaa tgaaagacaa acttttcagg
    16321 acaataagga attatctaga ggaaatgcga cgtgtctatg atgtcttgct ttctgatgtt
    16381 aatttttttg atgatagtaa ggaaagtgtg acttgtattt tgtcggggga acaattagct
    16441 aaaaaaatga gttcttacta tggtaaaact tatgacatag ataaatgtaa taagttacta
    16501 tcacttatga cttttatagg attgataaaa aaattagatg atgaagatgt tcctttgaag
    16561 atgctcgaat acctaaatag gttaaaggaa gagaaagcgt taggaaactt taatcataag
    16621 agaagcaatg tctatcgatt agacgttttt aatgcaactt ctgtgattca aaaattgaat
    16681 gaagatatcc ttccact
//
LOCUS       DACCQI010000068        16208 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCQI010000068 DACCQI010000000
VERSION     DACCQI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.069"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(127..354)
                     /locus_tag="IVO76_001785"
     CDS             complement(127..354)
                     /locus_tag="IVO76_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7429744.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(676..1929)
                     /locus_tag="IVO76_001786"
     CDS             complement(676..1929)
                     /locus_tag="IVO76_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7429745.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            1990..2730
                     /locus_tag="IVO76_001787"
     CDS             1990..2730
                     /locus_tag="IVO76_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7429746.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(2816..3574)
                     /locus_tag="IVO76_001788"
     CDS             complement(2816..3574)
                     /locus_tag="IVO76_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7429747.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(3571..4599)
                     /locus_tag="IVO76_001789"
                     /pseudo
     CDS             complement(3571..4599)
                     /locus_tag="IVO76_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(4619..7786)
                     /locus_tag="IVO76_001790"
     CDS             complement(4619..7786)
                     /locus_tag="IVO76_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429748.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(8010..8894)
                     /locus_tag="IVO76_001791"
     CDS             complement(8010..8894)
                     /locus_tag="IVO76_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7429749.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(8989..9426)
                     /locus_tag="IVO76_001792"
     CDS             complement(8989..9426)
                     /locus_tag="IVO76_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429750.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(9643..10554)
                     /locus_tag="IVO76_001793"
     CDS             complement(9643..10554)
                     /locus_tag="IVO76_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7429751.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(11011..11439)
                     /locus_tag="IVO76_001794"
     CDS             complement(11011..11439)
                     /locus_tag="IVO76_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429752.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(11467..12105)
                     /locus_tag="IVO76_001795"
     CDS             complement(11467..12105)
                     /locus_tag="IVO76_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429753.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(12109..14145)
                     /locus_tag="IVO76_001796"
     CDS             complement(12109..14145)
                     /locus_tag="IVO76_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7429754.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            14271..14936
                     /locus_tag="IVO76_001797"
     CDS             14271..14936
                     /locus_tag="IVO76_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7429755.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT         4870 a         3206 c         2638 g         5494 t
ORIGIN      
        1 tcatttgctt gccttcttcc aatcacttct tacttgatta atcataccat ttttttacat
       61 aaaaaataga tgatgctttt aaaaaaactc ctgttttctt ttatttaaaa taaatgtcat
      121 agaaaatcaa taatttaggt agccaccatg tgtatgcagt agcgtgtctt cttcccttga
      181 ggctttttgt ttttcgatac gttcttcttc ccaaacagac aacgctacta gaatctctct
      241 taatgatttt ccttcttcgg tgagcgagta ctctacttta ggcgggactt ggttataggc
      301 ttttcgatgg atactttcat cttgttctaa ctctcttaac tgctgggtca gcattttttt
      361 tgtgatcgta gggatttgct tgcttctcca cgcctaaaat aagcgatatt gctcttccct
      421 ttatctggtg taagcaaagc agttagcatt ttgacgatcg tgggttccgc ttttcacttc
      481 ttatctactt agtatcatga agtatacttt cgtactaata ctaagtagcg gaagtgtcaa
      541 ctactttgtt taactacata gcaaaagaaa cagaagggtg acaacggtca tcacgcttct
      601 gtttcttttg aatatgctgt gttgttgtct ctatctccgt gtagactttc ctcaaactta
      661 tcctttgaca gtaagtcagt aggctgattt gctctttcta gatgccagat acatttctac
      721 aaaaaaatag tgattctgtt ttgaaaattc gatttttagt tctgcatttt cgtaaacatc
      781 caacattttt ttcgcactgt atagacctaa cccgccattt tttttagagg tttttcctct
      841 tttgaccatc tccgatagcg tcaaattcgt tggatttgcc ttgttcgcta tactaaaacg
      901 tgatccctct ttttcttcca gcagattgat cgtaatcgga ctctgatcgg ctacacgatg
      961 atcgaatgcg ttagaaataa aaatagataa acagcgaata aaaacaacca gatcaatcgg
     1021 gattgttttc acttcgacag gaataactac ttggatcggg attttctccg attctgcttt
     1081 ttctagaaat tttaaaaaga cacccctgac agctggggat acaatatttg ttagctgttg
     1141 tatttgcggt ttttcaaaaa tagctgcaga atgatcttct aatatttcta aaaattccaa
     1201 cgtttctttt ttattttcct gaagagtaat ttttaatgaa aacagtagac ttttataatc
     1261 atgtttgaat tcctctagat gatggtaata ttcttcattt atttgataca gttgaagcga
     1321 ttcctgatgt tgtctttctt ttacttggaa actgatcaac ataatggaaa aaaacaaaga
     1381 aaaaaaactg atgactatcg acaaaagaga tagaagcaga taattagatg agcggaagtg
     1441 aacacttccc aataccaagt aaatcacgcc ccagcttaat aaaatacaaa tacctccaaa
     1501 atagtgttca gttggataag taaaaagcaa gttgagaaat ctcgtctgtt tgtctatact
     1561 ttttactaat agatagaaaa taaatgcgat aagtagtgca aaaaaacttg ctgttccaag
     1621 atgtctcttt cctaaataat ccatcagatc aaaaaccaga aaaaagctga gtccagtgat
     1681 agcaaaaaaa agattactga tcaaacagct gataaacagg tttttcgtag taaaataaaa
     1741 aagaaacatc aataataagc caactaggta aggaaagctc actttaggga taggaagtaa
     1801 aagtgtcacc accatcaaag ctatacaacc aaagctgttt tttactagca gttgtttcga
     1861 aaagaataaa tagataccta aatctaaaat caaagtaaag gtcaaataaa gtgtcactgt
     1921 tgcgaccatc ttattcctcc attgttttgt ttcaaaatgt acacatattt ttttaatagg
     1981 agcctgcata tgaaaaagta ttttatcata gaagataacc agtttcacag cgaatggatc
     2041 atcagagaat tggtcaatca tcatatcgag aatatcgtgc agctcacttc tatctctcaa
     2101 atcaatcagt tgatccagtt gggcactatt gataatcacg atgttttctt tatcgatatc
     2161 gacttgaagc aatcactcac aggtattgat gtcgccgaac aaatacgtaa aaaaaatgca
     2221 catagtaata ttgttttttt caccgcttat acgaattttg cagtagagat catcaatcgg
     2281 aaaatccttc cgattggata tatcagaaaa gacaggaatt tatctgaaca aattcgtcaa
     2341 gtgctagaac agatcaattg gaaagaacag cagatcgtaa atgtcgataa aatctttatt
     2401 gatcaaaaag gtggaagtct cgtcctgtat cctcgggaaa ttttatatat tgaaagtatc
     2461 aaaggtttga aaaaccaagt tatggtcaaa acaactacag aagataaatt agtgaatact
     2521 tctttaaaac aattgaagac agagttagaa aagtatgctt actttttact tttaaaatct
     2581 tactgtatca acttatcgca attgcagaaa attgattctg ctcattatgt gttagaattt
     2641 ttcaacggtg actccttact cgttggtcga aagatctttg aaaagacaaa ggaacgtttc
     2701 catgacttcc aaaaaactgc ttcgagttga tgaataagag ctgagacgta agcatagcag
     2761 atttcaggga gtgggccttg aatcggctcc actccctgaa atcgttgata tgattttacg
     2821 cttcctcttc tgtaggagca tctgttaacg accatgtgaa tgttgaaacg tagttgtcta
     2881 cagcataatt ggatacagga acaaataatt ccactgattg tctcccttgt tccacattct
     2941 ctcctaatga ataaatccat gtgccaatcc cttcatttgc tcctgcagtt actagatcta
     3001 ctgcctgatc tgctgggatt tcgattcctt cagcgccagt tccagaaacg gtagatggca
     3061 ttccactgct attagttgcg ctatttcgaa tcacggcgtt tttgaatttc acctttgaac
     3121 cagttagttt attcgtatca gatgttgtac caataaattc tgttcgtttg actgataatt
     3181 tccaaccggc ataatttccg cttttatccg ttacttgtac gaaattctgt acttgtgttt
     3241 gtgctccagt actgtccgtt ccgctttgta atgcagcata tactgttttt gctgtaccta
     3301 acttgattgt tccaaaatca agatctggag cttggtcaat cgtcagtaat ccggatgttc
     3361 cctgatttgg ttcgtcaggt gtaaaaggag tatcaggatc atttggatca acaggaggaa
     3421 ctggaacaac gtcttcagga tttgcagcat ctggtttggt cacagaaatc gttccttctg
     3481 tagtggctga gctcttattc tcagcaaaaa cactttgtgt acataataat agacttccag
     3541 ctagaacggt acttaatgca attgtttttt tcatttcttt ttccttcttt cttttaaaat
     3601 aaataatcca gcaaataagc caataagaat gactccaaca agtatcagcc atcgccagtc
     3661 aaatgtttct ggctgatttt ttaaatcaat atccttttca ttcaattggt ccgctgcatc
     3721 atcagttatt tcaaaatcgg tatggagatt ccagcgtttt tctttccaag cacccttcac
     3781 aaccgcttga taatgtcccg gagctaattt ttttccttct aatgggacaa gtaaatcaaa
     3841 atttgtattg ggtgctacat ctattttttc tttttttatt gaaaggtaag gctcttttgt
     3901 ttcttttttg tagatttcaa ccgatgtagt caagccgaac atataagaag catttggatt
     3961 ttgaagattc gccttaatca catttcttcc atcgctttga tccgcataag ctttttttaa
     4021 tttcaaatct ggggaagact ctttttcgct atttcaaatc aagattgctt tcacttgaga
     4081 aaactgattg acgacaccaa ctgtactttt gttagaaacg ttctcttttt tttcttgaaa
     4141 cgtgatccct cctgcgacca caccagccag ctctttttct ggcgctttca gtgaaagtgt
     4201 ctcagtgacc gcactttgag gaggaacagt tatttctccc actgtttttg ctaagttttt
     4261 catatcataa cttaatgttt catcaaattt actttttgat tctccatatt cgatcacacc
     4321 gtttgaattc gtcgtagcgg agtggatcaa tgtctcaact gttactgatt tatctgttcg
     4381 gttaaagacc gtgtagggga tttcctgtgt tttcccaggc tccatcctta catttatata
     4441 tgttttgttc ttatcaatct gattagccgg taattcaaaa tctaccgcaa aattgaattc
     4501 ggaggcatgg acaggtgtct gccaaaggat gaatccaagg agaaggatca taagtaaaga
     4561 gcagttgttt cgatgacttt tattccatat tcgattcatc aatcatcctc cttgatcttt
     4621 attctgtcgg cgcatctgct aatgtccatg ttatctctgc tgagtaatta ccagctatct
     4681 gattttcttt ggttaagaca agtttaaacg cccgaccgtt atttaaatat tctgaataat
     4741 ctgcttgcac attcgttgaa ttttgtcctc tgacaatttc cagttcttct tcattcacta
     4801 attgatactc ctctcctttc ttgaaatata ggacatcctg catagaaaat cctgcatcat
     4861 ttgtgaaagg ctgagataat tttatcgtta agatccacgg atttttcgtt tctcgctgat
     4921 cataaatcca taatttttcc tgtatatcca tttggccaaa tgcttcttgt gtcaaaccag
     4981 ttgctttgat ctgaccaaaa tcgagtttgg aaggggcaag aaatgagaga ccaccttcta
     5041 ctacgatctt cgtagcaggt gcaaaaggcg gggcatcatg ataaacaact ggcgtatcac
     5101 ttggattgtg attcccaatt tcatcgttgg tacttggctt gttctgcaca cccttagcag
     5161 tagcttctcc tgcttggtcg tttaatacaa tttctaatac atctcctgca aaaagttgat
     5221 tttctgggat agaaagaaca aattcccctg tatcatcaat agttgtttct gacaaaacag
     5281 cttgattttt tttcaatgaa accaaggctc ctgcttcacc tgtacctgtg atcgtgcgag
     5341 aagctgcatt gatcaatgtg gtacttaaat tagcaggttc aggaggaatc acatcgactg
     5401 tcgttttggc agagtccagt tctgtcactt cttggccacc tcttactttt ttagcagcaa
     5461 caacttgcac ttgatcctcc gtttttaatc tctcatcatt gggtacttga acttcgaaca
     5521 agccgccttg ttcgccttct ccccatgggt tatatcctgc cccttcattt tcatctgttt
     5581 gtccttgcgt gagagcagta atttctacag atgatttcgt cgtatctgcg taagtaatac
     5641 gtaaagtcac ttctacttcg cctgtccaag catctcttgg ctttgcctcg acaccttcgg
     5701 gaacaacgac atgcccatag atttttttat ctgcattcgt tggcactctt aaggattcaa
     5761 tcatcgggag ctgattattg gcactgattc gattataatt tttcattccg gcacctgtct
     5821 gagcaaattc atttgttaaa cctgcactag ggcttgatac gaaattgcct aagtcggtcc
     5881 cattgatcga taagtctgtc atatcagaaa aataatccgg gtctttttca aagttatcga
     5941 atgttgcgcc tgtttttcgc cataaagcga tttctgaatg ggcgatcttt aaagaagaat
     6001 ctccatttcc ttggaatacc cagccgccta tgccattggt ctcgcttcca atccgtttgt
     6061 tttggaaatc aaaataggta ggagattcta agtccacatt caacacatcc ccactgatga
     6121 tgccggtctt atcttcccct gttttcccga ccatgactaa ataagttttt tcacctgccg
     6181 taaaatttaa atcaatatct ccagttgtat ctacagttgc tccggaatct gcgcgtatat
     6241 cgatatttga tttttctcca gtgactgtaa aatcagctga acctggactt tctagaaaac
     6301 cagaagcgtc ataaaattgg atcgcttgat ttccttgatt gagtctgttc acgtaagatt
     6361 tgccatcacc tgtatttctt accctcaatg agcctccatt ggttactgtc agcttttgcg
     6421 taccattctc aaaacgaact gcgctggaac gcccactctg cttatcgatg tcgataactg
     6481 atccggctcc ttctacgatc aaagaaaggc cttttcctac aaaacgcatc ccattgttcc
     6541 cgggttgatc gtcatctcca gccattttga ccgagatttg tccaccactt gatacatgga
     6601 tttgactaga atctccttgc aagcgaaatc cgcccgtagt tgctgtattg atggtgattt
     6661 tcccaccatt cgatacagtg actttcgatc ctgaacctct catttggatg attccgcgat
     6721 tgttgtcatt ggtaatattg atctttgtat ttaccgaaaa ttcgcttccc gaaccactga
     6781 cttcaaacat gacattacca ggtagcgtat cgactgcctc tccttcatcg ctaacgccat
     6841 ctgcccgatc ataatcaaga gagacctttg aaccgcttaa gatatggatt cctggcgctg
     6901 tttttccact accagtctca tctcctgaat ctagaaaacc gtagaatttc cctagatacg
     6961 gtccataaag ttgactacca ttgatcacat tcaattgata gcttctacaa aaataggctt
     7021 tacccggagc ggcattcagt ccttccttca ctggttggac atccaacaca ccggaaacac
     7081 catctaatgt gacagttgca cgaggagcgt aaatcagtcc taatttggac ccatcataca
     7141 aatcaagtgt cccggtaaat tttatttctc ccgtaaaatg atatctgctt ccccaaaaat
     7201 cggtcatata agcaatgata atggcatttc catcgatatt tggtgtcaat ccacctggat
     7261 gtgcagctgt tcctttaaaa ttctggaact cgatcgactg aggtatctca ttgatataaa
     7321 tatggtgtgt gccgaaattg tgagcgaatc caccaaaatc aataacgata tctcgtttga
     7381 tctctaattc cctgtctaaa gtcaaaggct gaatcagtcg gatcttagtt actgattcgt
     7441 ctgcgagaag ctgcgccagt tcttctgttg tggcagcttc gctgtaccca tccgctgaaa
     7501 cagctcttgc cagtgccgct tcactgctat cagacgtttg ttcagctact tctgaggtct
     7561 cacttgtctt attttcttct tgccttttat tagtagaagc ctcactttga gattctgtag
     7621 tttcagaagt aatcgattct tctgaaaaag tagtcttgtc aaaactttga ttctctgatt
     7681 gcgttgattc gtttgttttc tctacatcgg caaaaacgga tgtcaccatc aagagaggga
     7741 agacaaacaa aatcgacgca atcagctttt ttacacattg attcattctc caccatcctc
     7801 tctgacgttt tgacaatttt attttatcgt tttaatctat ttccaattgt actttcatat
     7861 ttaaaatatg tatttttatg gaaaaatttt tctattaggt gtaaatttct tttttgtaca
     7921 aaaaaaaggc tgtgacaaaa gtcttgtcac agtcccttat cccgaacaag cggtgttctc
     7981 ccgccaactc ctcggtcaca acctccattt taacaataaa cctgtaaatg tgagggcaat
     8041 actttgattg tcacaggtaa tttatcccct tcatctccat caacattcgt cgtcaattct
     8101 tcctcatcag ctaatgacac aactaattgc tcacaagtaa ggtaggctag atttttcgta
     8161 gattcatcta ctccttttaa taggtctggc aatgttttaa gcgtatcgat caaagaagaa
     8221 tcttttaaat aaaccaaatg gagcttacca tcatccactt ttgcatttgg aagtaatgtt
     8281 tcaaaaccgc cgactgagtt agttagtcct atcagcaagg tactgctctc aatttcttcc
     8341 ttctttccat caacttctac gtgaaacgag taggattgtg tggaagctaa ctgtttgatt
     8401 cctgacatga aataagctag ttttccaaat ttcgtcttct tctccgcatc tacatcattg
     8461 attgattcag ggatagagcc aattgcgacg acattcataa aatagtcgtc atttattttt
     8521 ccaatatcca atggcttgac tgattcaatc gaaaaatgat tgatcgcttc ctcaggttct
     8581 agcggtatcc ctaatgctcg agccaagtca ttcaccgtac ccaatgggaa aaatccgaaa
     8641 tttggccgat gttcttgttc agcgattcca ctgattcctt cgttgactgt tccatctccg
     8701 cccatgacaa acacactatg atacccttcc gtagccgctt cgcgagtaaa gttttttgca
     8761 tcccctgctt tttttgtatg caagacgacc acttcatcaa acaccgattc caattttgct
     8821 atcgctaatt gctcaaattc ctttgcttgt tcaccgcctg aactggggtt taccactaat
     8881 aaaacttttt tcatcacttt ctttcccttc tgattgattt ctttaatata acgatagcat
     8941 aaagaaaaaa attaggctgt gatgaagtac tgttcatgaa acattcattt attttttgac
     9001 aagataaaac atccatacta tgaaactgag gaaaattaaa acaatgatat aggaaataaa
     9061 agaaaatgat aggcattgta tacctaataa agtaaaaatc ttaggtggca gataaaaaca
     9121 caaaaatagc aagagaatgt aatagcgttt cttccacttt ttgttatcag accttatagc
     9181 gatcagagga ataccaaatg cataactgaa aaactcttga tgtactgtta atatgtcacg
     9241 gccttgaata ataaatgcga caatccccgc tataattagt gtgcctaaca ccaaaacgga
     9301 aaattgatga atatatttta tatacaaatg atccattgta cttcctgcct ttttatttgt
     9361 gagaaaaaca aatccgtata cgtgaataac ttctagaaat aaaagaaatt tgcaaaacca
     9421 ctccatttat ctacttctac gtgcattttt tgatattgtt ttctttattt aagattatac
     9481 agaaaaatat ctagggtcgc tggattctaa gtaaaaaaaa tttttaaaat aaaaagaact
     9541 aaatcaagta gtaattaaac tactgttgat ttagttctaa taaaaagatt tagatgaaca
     9601 gttttattga tttaaatagt tcatattttt tatttatacc gcttatttac tacttgctgt
     9661 actagaatct gaagtgaatg tactcaaaac attttcaaag tctttgtctt tgatttttac
     9721 attagcgtct ttcaatactt cgccaattac tttattctgg aaggtactgt cacttaattt
     9781 tgtgtctgtt gcaatttctt tcaattcttt tttgtattta tccatgtcgt tgcctttgtt
     9841 ttggtttttc accattttaa ccacataata ttccgtagta taagttgaag cattggtaga
     9901 agtgatcaca tcagaaactt gaccatcttt caacttaaag gctgcttctt tgacttctgc
     9961 cggaaccgtt gtagtagtgg aatcaaattt cacttttccg ccatcttctt ttgtcgtagt
    10021 atcagttgac ttgtctttgg ctaatttaga gaaatcgtct cccttatcag cagctttctt
    10081 cacatctttt gcatcatctt cacttgctac ttggataatt tgagcttcca cttctggatg
    10141 gaaagattcc caagccgttt ttaaatcttc atcggtcaat ttgatatgtt tctttagacc
    10201 ttcttgaaag gctagacttt gtttgatact gtctttgaat gtttgttctg tgtaacctgc
    10261 agatttcaat tgtgaatcaa atgaatcacc caattgtttc tttgtttgat catattgaga
    10321 atcgatttct ttggttgata cgtcatctcc atatttatct tcaaatactt gataaacgat
    10381 caactgttgc acagcggaat ctgctccttg catttttttc aagctatcca ttacttcttg
    10441 ttcgctgatt gtaccgcctt tataactaac ggctgttttt tcaccggcat tcgtacagcc
    10501 tgacaataag ccagtgccta gtacgatggc aagtgctaaa aatctttttt tcatttatca
    10561 attctcctac ttttcgtttt ttgcgaattg ctaaaaacaa aggtgttcaa gatgaaaatc
    10621 atcaagaaca cctttactaa tctgctcgat acgtcctgaa tagatataag gctacacaat
    10681 ccagaaagtt cgctcagcag aataaatgtc aagtagcaca tcacacctta atatactact
    10741 tcttttgtta ccgtttttca agtctatctt gcgttccgta tttaacgtaa ctaattctta
    10801 gaacaaatgt tatcgatttt gtttttcttc ttccgctgct gggatattca aatcagacac
    10861 ttttatgttc gattttccag acattttttt gttgttttta ttatgatata tttttataca
    10921 actaaaaatg aatatattaa aactaccaaa aataactttt aacgctgttt cattatatgt
    10981 tttttctatt ttatataaac ttttggatac ttaataccaa ccggttggat tgtaccaaca
    11041 tgagaatgtg tcagttctat ttcctgtcgc acttgcatag gcccatactc cttttgttgc
    11101 atttgctgat cgaatagagt ttccaacttt cgcttttgca ccgtgacgat tatagacacg
    11161 actatagtgg ttagaatgac ctgtttttct cgtacaagtt gtatcactat aataaaccca
    11221 tcttccagag ctatgattaa ttgctgttct agaaatttcc cggtctactg gtaaatttga
    11281 cttaataata ttacctttag catcaaaatg aatctctcct ggctttaaag atttgtaata
    11341 gatttcaccg tgaactgaat ttgcttccac ttcagatgac atagcgtttc ctccaacaaa
    11401 acctatccct agtgcacaaa taataactgc ttttttcata aaacgacatc ctttcctttt
    11461 tgatttttaa agcatgatct tcttgtttcc tattttggtc agatatggat catgcgtgac
    11521 tacgataact aacgtacctt gggaagcttg ttgcgataag atttcaacta ctttatcctt
    11581 atttctttta tccaatgacc cagtcggttc atcggctaaa atgattttgc tattcttcaa
    11641 taagactctc gcacaggcga ttctttgctt ctcaccacca gataatgtgt agataggcgt
    11701 acttagcttc ttgtttatat ttactttttt caatgcctca tactttgctg ttcgcttatc
    11761 tgaaacattt aaattcgaaa acttcaatcc aatatctaaa ttaaaatcga ttgtttcata
    11821 ttcaatcaac ccaaaatctt gaaaaagaaa agatatggta tttcttttta tcaagcgctt
    11881 ctctctccca gttaattgtt tcgtctcttg atcgaacaaa tagaattgtc ctgaactgaa
    11941 ctgatcattc aatcctaata tattcaataa cgtactttta cctgctccac tttctccaac
    12001 aacagtcaca agatccccag catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt
    12061 tcgaatgatt tttttcgcac cttttatcac tatcaacggc tccaattgtt aacctccatt
    12121 catcagcaat aactgacttt tcttttctgt agacaaaata aaaacaactg aaagaataaa
    12181 atcaatgaag ctaaagaaaa aaatattaaa aagatattta ggctctcttt ctataagaaa
    12241 aaggaagatt acacttgtcg ttccccattt gatgaggttc atcatgaaga aatctttata
    12301 cgtttcaaag agcgaataac cacttgaacg aaagatagcg attttccgac aattgtagac
    12361 aaaatatatg cataacatga catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa
    12421 tatctgtatc aagtaatgaa atgatctgct taacactttg acagctatct cttcagtctg
    12481 agtatacgga aatgataact gcaagttatc acgtaactga ctttcttcaa tcagctcaaa
    12541 taacctttct ttcgacaaag agcctatctt gatctttaac ggtggataga tttctccgct
    12601 gagaatattt ctttccaaat acatactgtt tttcaatgtc agtatttcaa caaccggata
    12661 atgttcgatc caggggatag aaggaataaa tgtataaatg ggttgattgt tttttataaa
    12721 tagaaatttc ggttcttttt cactgatttc ttgataatat tctgttattt gcgccaggtc
    12781 attttttaaa tctgtatgtc tatatcgttc agggatcaat ataacagggt gcgtttcttt
    12841 ttcggttatt tggatcaacc ttccagagac atcaatcaaa ggaaatttct taagataatt
    12901 cagatttatt tttatccctc tgccaaactg ttcattttct tcattcaagt aatctgatac
    12961 attgactaat atacttccgt ttttattcaa agtttcatat aattcctgat ctgctgctga
    13021 tgcaaaacga ttggaataaa taaaatccac gtggtttttc ccgatggtta ctggataaaa
    13081 tacatgatag tcctcaccag cgatttcttt tggtggttca tgggaaacag ctaactgtga
    13141 agaagaaaaa acgatagttg ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa
    13201 caatccaaat gtaaaaagaa tgtattggat atagttgaac gcttctctta acgaagcgat
    13261 tttttcaact aataaaagag aaatcaaaga gataccatat attccgctga acaagaaaac
    13321 gtacatctgg aacaaataaa gaaattgctc cttataatac ataaaaataa aaccaccaat
    13381 gattcctgca attatgaatg taataatgat tttctttttc cccaataaac accacaatac
    13441 ttgatgtaat gaaaagtcat tattcaacaa taaactgatt tctttattca tcaataagaa
    13501 aaaggatgat aacaaaagga ataaaagaat caatccaata gttaaataca ctcgataatt
    13561 atatacgtct gtatttgaaa taaccgaata cgaatagcta ttttcaaagt cttcaaaaat
    13621 attagtggca taatcagtgt gaaatgcttg attgaatcga tcagtgaata gtgaaatgaa
    13681 ccgttcatag gcttcttttt ctctcgtcat caagaaaaat tcaccttcaa aatcttttat
    13741 tttgatcgat tcttcaatgg acgttacgcg aaaggaggta ttagaatagg gtaccttctc
    13801 gtgagaagat ttgacatccc cttgataata caatgtcatt tcaagctttg gctgtatctt
    13861 gaagttccaa gatgtatcta cttcattgga ttcatttact gtttttttca tataagaaag
    13921 ctcagtttct gatgagacat tttcaaatac catgattagc tgctcatatt cttcttgccc
    13981 aataaaatgt tgtggcaatc gaatcggagt ataagtatct gtttgatgga aaaattcatt
    14041 tgtccgatag agtacctgtt tatttatact aaccaaggca aaaccactca tgataccggt
    14101 cactatgatt gtgagtaaaa ctgtgaactt ttttttgatt atcaccactc tcccttactt
    14161 aaatgattct ttgataaaat cataccgttt ttcatcaaaa aaacgtatct atcaagtgta
    14221 taggtcatgt agaataaatc tatatactgt agaatcgaaa ggagaagaag atgaacaccg
    14281 gagaaaaaat acgaacttac cgcagaaatt gtaagcttac gcaaaaagaa ttaggcgaaa
    14341 aaattggtgt ttccgataaa acgatttcta gttgggaaaa ttctcgtaca atgccagacc
    14401 ttgaaatgct gtcactttta catcatactt taggtctgcc gattgatttt ccttctattg
    14461 aatcagctac cgaatcaata gaaccaacat tgccagatga acctcttgtt aacaagaaat
    14521 cttattcatc aaaacaaccc ttctcactca aaaagacact atctttcttt ctgctcgtct
    14581 tttgtttggt ctatcttttt cttatcaatt atcaatacaa aaatgaaacg atcgatcgat
    14641 tcaatccttt tttgaaacca caaatcggct ataccacact ccctgtagga tacgtggaca
    14701 attccatcga acgttgggta tgtaatgata tctttggcca tggtacatgg ctgtcttttc
    14761 acgctggaga ggtagaaaaa gagaaaaacc atgctgtaat caaacacaaa ggaaaacacg
    14821 tcatgaacgt tcgcctgctc acttgggaag ccattccctc agattaccaa gaaatcctca
    14881 gccaagacta tgacgacgcc ttaaaaagaa gccctcagga gccagatagg cattaagtag
    14941 gagatgaacg tagagataga aaaaatcaat gcacatgatc ttatccaaaa aacaggggta
    15001 caaattaatt gaaaaagcct tgagatgaaa gttcctaaat aaaaaagttc agaatgatag
    15061 gtatttctgg cttatagtat aatatttacg ccacagcctc ccaatgattc tttttggaat
    15121 cataatcaag gatggacatt cacaaaaata ttttgctcat tcgatccgaa tagattaacg
    15181 accaatctag taagtaatat agattggtac gtagtcaagc tgcacataat ttagatcaat
    15241 tttagttctt ttttatattt ctatgatctg ctcatttact tttatatgat ggcttacagt
    15301 aacataaata aatatgataa tcgctggtag tttatccaag agtactcaat aaaatacaaa
    15361 atggattgtt caagcgccta tcataatgat aaaatgaaac aaataaacga tacaaggagg
    15421 aagagacttt gagtaatggc agaacaattt tttcattgta aaaattgttc agttgttaat
    15481 ctatgggatt tcggtagcat gggcattttt ttatagagga atcacaccaa cctggccgtt
    15541 ttatctcttg ttagtgggaa tattagtagg aaattttgcc cctcttcact atcgtacgaa
    15601 cttttttcca actgattctg gcctttttct taaaacaaag acgcgaaaat tagaaaacat
    15661 tattgaaatg acaattacct gtataatcat tttcttagtt atattcctaa acgtcttcta
    15721 ataagaatct cttaaaatta gcttgttaca gttagttacc tagtttatat aacacagtta
    15781 tcttatcttc cttatcaaca tcttttgcca actctttgtc ttaaattcga aacatataag
    15841 cagagctacg aacaccaaga ctgtttcggc tctgtttttt gcatcaaatt cactcttata
    15901 tagctgataa tctcggctct ttaaaagaaa ttattttcgc ttcattttat tataaaaagg
    15961 ctaggaacct tgatgttatc aggttcctag cctttattta agtattaccc cacagaccct
    16021 tccatataga tttttaaatt acacactagt ttaaactaaa acaaacaagg aaacacttac
    16081 catttaatca taaaattagc tcatatgaat atattagaat gtaaaataga ctggtcaaaa
    16141 tctggtcaaa aaagaggact aatacaggta gtcctcagac tgtagacaaa gtccatttta
    16201 tggatgat
//
LOCUS       DACCQI010000069        16198 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCQI010000069 DACCQI010000000
VERSION     DACCQI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.070"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(191..1342)
                     /gene="queG"
                     /locus_tag="IVO76_001798"
     CDS             complement(191..1342)
                     /gene="queG"
                     /locus_tag="IVO76_001798"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7429756.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(1361..2113)
                     /locus_tag="IVO76_001799"
     CDS             complement(1361..2113)
                     /locus_tag="IVO76_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429757.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIHFVQLKKEKLYYGLYSR"
     gene            complement(2253..3203)
                     /locus_tag="IVO76_001800"
     CDS             complement(2253..3203)
                     /locus_tag="IVO76_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7429758.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQIKES"
     gene            complement(3204..4043)
                     /locus_tag="IVO76_001801"
     CDS             complement(3204..4043)
                     /locus_tag="IVO76_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7429759.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(4040..4786)
                     /locus_tag="IVO76_001802"
     CDS             complement(4040..4786)
                     /locus_tag="IVO76_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7429760.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            5024..6394
                     /locus_tag="IVO76_001803"
     CDS             5024..6394
                     /locus_tag="IVO76_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7429761.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(6449..7345)
                     /locus_tag="IVO76_001804"
     CDS             complement(6449..7345)
                     /locus_tag="IVO76_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7429762.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(7626..8303)
                     /locus_tag="IVO76_001805"
     CDS             complement(7626..8303)
                     /locus_tag="IVO76_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7429763.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(8394..9299)
                     /locus_tag="IVO76_001806"
     CDS             complement(8394..9299)
                     /locus_tag="IVO76_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7429764.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            9474..10928
                     /locus_tag="IVO76_001807"
     CDS             9474..10928
                     /locus_tag="IVO76_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7429765.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            10944..11975
                     /locus_tag="IVO76_001808"
     CDS             10944..11975
                     /locus_tag="IVO76_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7429766.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            11982..13169
                     /locus_tag="IVO76_001809"
     CDS             11982..13169
                     /locus_tag="IVO76_001809"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7429767.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            13325..14707
                     /locus_tag="IVO76_001810"
     CDS             13325..14707
                     /locus_tag="IVO76_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7429768.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            14679..15065
                     /locus_tag="IVO76_001811"
     CDS             14679..15065
                     /locus_tag="IVO76_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429769.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(15265..15933)
                     /locus_tag="IVO76_001812"
     CDS             complement(15265..15933)
                     /locus_tag="IVO76_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7429770.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT         4662 a         3252 c         3021 g         5263 t
ORIGIN      
        1 ttctatgttt ttttattttt gaatgaaaaa cgcagattct gtaacattta ctgcaataat
       61 ccataaaaaa aaagcaaaaa aatttttgat ttttatcaaa ttctcttaaa aaaatgtaaa
      121 aaatacaaaa aaaccagtaa ttgttacaac aattactgga caaaaacttt gaataatgtt
      181 aactattttt ttattttaac tttctccgca ataatttctg ggcatgttcc aattcgttta
      241 taacgacttc ttctgtttct ttagctaata tttcatctaa tacatcaagc catttttccg
      301 gttcttttgt tcctagacgt ccaatagccc aagctgctgt tcctctgatg acagggcgga
      361 catctgttaa acattctgaa atctcaggca atgccgttcg atcacctaga ttagctaaag
      421 caatcaatgc gtttctctgc aaaggtttct tcccacgcca tgaaccagct aaatgcccaa
      481 attgctgttt gaagtcttta ttagacatcg aaagcattgg ctttaatttt ggatatactt
      541 cgtctacttt tggttccatc tcttcatgaa aatgaaaatc ttttccttga ttgtaagggc
      601 agactaactg gcagatatca catccataaa tgacatttcg cattttttta cgatattctt
      661 ccggcatcat cccttttgtc tgagtttgat aagacaaaca tcgctgggca ttcattcttc
      721 cgtcaccaag caatgctttt gtcggacaag cagtcacgca gcgcatacag tcaccgcaac
      781 caaattctcc tggtttatca ggctcaaata caatattagt aacgatctca ccaagataga
      841 caaatgaacc gaactcttca gtgatcaaca atccgtttcg tccaataaat cctaaaccag
      901 ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc tacttgcgga gctagacgcc
      961 attcctcttc tttttgccat ttttctgctc ttgtttggat gaattcgatc aatcggttca
     1021 aacggtctct gagtatatca tggtaatcga tcccccaaga agcacgagca aactgcccgc
     1081 gtttttcatc cctaggcact tcttcatgga tcctagtagg atacgcaagt gcaatggaaa
     1141 tgatcgattt cggctgatca aatgtcattt ctggataaag acgctcatcg atatttggat
     1201 gttcaaagcc agaagtatgg ccagctgctt tttgttcttc caacgattct tttaatgaat
     1261 caaaaggttc cgccgtggtg aacccgattt tgtcaattcc taatcgtttg ctttcttgta
     1321 tgatttcttc ttttaatgtc atggtcttcc tcgcttactg ttaacggctg tataacccat
     1381 aatatagttt ttcttttttc agctggacaa aatggatctg ctgatcggca atcttctttt
     1441 cttccatcca tttttttctt gcagcaaata cttcccaata atttgttgaa attaattttc
     1501 ttttgttatc taatgcatta aaaaaattca atgaaactgg ttttgtactt aacagttcat
     1561 ggagccttac ctcttcccat ggcgaaaact cagacagatc gcgggaatac aatacttctt
     1621 catagagatt tttttgctgt gtgtacgtga atgtttgcgg gattttccca ggcctggcaa
     1681 tttctactag acgattataa aacatattca ttaatttgct gttggcaata aattgacttt
     1741 tataataggc cgactggatc tttttgatta ctccttctgc tttgatgact gaaaataatg
     1801 caggccatgc tttgacggtt atttcgtcgt tttgtttaac taatacgcca ggtgtgaagc
     1861 cgactggaga aaggaacaca aaataaccga taccggtatt ttcatctaaa ggctgaaatt
     1921 catcaaacaa ccatttgcct atgtacagct cttcatctgg ttctgttgtc tcattttgat
     1981 ttgctatgaa taatttagct aattctgctt caagaaattg aaggtcttga tccttgtcgg
     2041 tattttcctg ctggctgatc aatcgaaaga cttcttcttt aatcgtatat ttatctttac
     2101 gtgtaaaatt catagccaca ccttccttcc ttactctaac tataacggaa tttcttcaaa
     2161 aaaggtatct gtacgccgaa aaacagcata atttttttca tatctcttat aaaaaatgca
     2221 aggcctgctc ctcaagctag accttgcact tttcagcttt cttttatttg tgacaaacct
     2281 tcatggatcg tttcaatatt ccatttcatc atctgatagt aagaatctcc ttgttctcct
     2341 tcttttgcta tggaatcagt gaacagttta tcataaattt tcaaacccgt ttcttttgct
     2401 acccgttcca tgcttcttga gtctacactg gtttccacga ataaaaccgg tacttctttt
     2461 gctcttatct gatcaatgat cgctttcatt tgatcaggcg taccttgact ttctgtattg
     2521 atctcccata tataagctgc tggcaaatca tatgctcgtg aaaaatactt gaatgcccct
     2581 tcacttgtta ctaataattt tttgttctct tcgatagaag caaaactttc cttcgcttgt
     2641 gtatctaatt cttttagttt ttccacatat gcttctgcat tttttttata gagtgtcgca
     2701 ttttctgcat ctttttcaga gaatatacgt gcgatttcct ccacatattt tattccgtta
     2761 gataggtcta gccaagcatg gggatctgct tttgtatgtt cttcaccgct agttaaataa
     2821 agaggttcta catttttgct aactgcaaaa taatcttttc cttcttcttt tttagccgtt
     2881 tccatcaagt tatcgaacca gctgttacct gtttcaagat tcaaaccgtt gtataaaata
     2941 acatccgcat cgcttgcctt tttgatgtct tctggtagta cttcatattc atgcggatct
     3001 gttccgactg gtacgatact gtggatatct atttgatctg ttccgacctc ttttgccatg
     3061 tccgccaaga tcgaattggt agccacgact gacaactttt ctttacttcc ttcatccgct
     3121 tggtcagaag cctgctttcc gcaaccggct aataccaaga aactaactgc caagattaaa
     3181 aagagtgatt tttttgtttt catctatctg cttcctttct gaaataaaat tccttttgtt
     3241 ggagaaaata aaaaagcaag gataaaaaat gcggtacatg ctaacacgat cgtagctcct
     3301 gatggccaat tgtaactata actgaaaaac acaccgacaa ctgcacttag cataccgatt
     3361 cctgcagctg taatgatcat tttcagtaaa tggttcgtta gcaagtaagc cgtggcggct
     3421 ggtgtgatca acatggcaat caccaagatt gttcctactg tctgtaaact gactacagcc
     3481 actaatgtca aaaagaacat caaaagataa tggatcagcg atgtgttcag cccataagct
     3541 tttgccattg tcggatcgaa tgatgtgatc ttcaattctt tataaaagaa aaagacaaat
     3601 atcaagacga ttccactaac gagtgctgtc agcaacaaat cgctttccct aacagctaag
     3661 acattcccaa ataaaatatg atacaaatct gtcgcactct gggcatatga aatcaagata
     3721 atccctaatg caaaaaagga gctaaagaca attccaatcg ctgtatcatt tttcaatcgg
     3781 ctccgctgag tcacgaaacc aatcactgct gctgttagca tcccaaaagc agtggctcca
     3841 aagatgtaac taaaaccaaa catataagaa atggctacac ctggaagaac tgcgtgagag
     3901 atagcgtctc ccatcaaaga catccctcgt aaaataataa aagaaccgat gactccagaa
     3961 gcaagtccta caagcatgga agtgaccaaa gcgtattgca ggaattgata attcattaat
     4021 ccatcaataa aggattggat cattttttct ctcctccttt tactaaatgt ctgatgatct
     4081 caccataggc caattgaagg ttcttcgatg taaagacttc ttgtactgga ccggaagcga
     4141 tcagctgttt atttaaaaga ataacctcat cgaaatattc ttctacttta tgaagatcat
     4201 gatggacgat caaaatcgtt tttcctgaat tcttcaattc ctgcaagatg tcaaagatct
     4261 ttcgttcact taacgcatca atccctacga atggttcatc taaaaagatc cattctgctc
     4321 cttgggctag agctcttgca ataaaaactc tctgaagctg tccacccgat agttcgctga
     4381 tctgtctttt ggcatattct tccatcccta cttttttcaa agcttgtctt gcacgttctt
     4441 tttctttttt cccaggtctt tgtccaattc gtaaagatgg atatgttcct aaaagtacaa
     4501 cgcctaatac atcaattgga aaggaaagat ccaattcact tcgctgttcc acgtaggcta
     4561 tttttttacg gactgagttt accggctgtc cagaaaaggt gactttacca gtagtagctg
     4621 gtatcaactc tagcaaagat ttcataaatg ttgattttcc tgcaccgttt ggtccgataa
     4681 taccggtaat tttttgtgga tttatcgtca agttgatagt tttcaaaaca gttttccctt
     4741 gataagcggc tgaaaggtct tgaattacaa tggccgtacg ttccaaatct tacactcctt
     4801 tttaggttta cctaactttt tatttaaata tagtttaact aaaatggaaa gcattttcaa
     4861 gtagaatgat gaaaaagttt ttttcgaatt ttcctcgcat tttatgaaaa ttttctgtag
     4921 aatcacatca agagttcaat aaggctaacc taataatgaa aaacatgtgt tgaaacgttt
     4981 gattattttt tcttgttgac tcaacaacga aaggagtttt tgtatggaag aagaaaagtt
     5041 aactcgaagt cttagtgccc gtcatatcca aatgatcgca ctaggtggaa cgatcggtgt
     5101 cggtctgttt atgggtgcct cctcaaccat acgttggaca gggccctcag tgatgggagc
     5161 ctatgcgatt gcaggattct ttttgtattt gattatgcgt gctttaggcg aaatgcttta
     5221 cgttgatcct tcgactggtt cttttgcaaa ttacgcgagc gagtacatcc atcctgtggc
     5281 tggctacttg accgcttgga gtaatatttt tcaatatatt gtcgtaggaa taagtgaagt
     5341 gattgctgta ggatcttata tgaattattg gtggcctgat ttgccggcaa ttattcccgg
     5401 aatcattgtc gtcttgtttc ttatgctggc aaatcttatt tctgtcaagg cgtttggcga
     5461 attagaattt tggttttcga tcatcaaagt cataacgatc attttgatga tcattgcagg
     5521 tcttggggta atcttatttg gatttggtaa tcatggtcag gcagtaggga tttcaaatct
     5581 ttggaaaaat ggtggattct tcactggagg cgtcaaagga tttttctttg cattatcgat
     5641 cgttgttgct tcctatcaag gaatcgaact gatcggaatg actgctggtg aagcagagaa
     5701 tcctaaaaag acgatcattg aagctgtcca gtccactatc ggtcgaatct tgatttttta
     5761 tattggtgct atttttatca ttgtgagtat ctatccgtgg aatcaattga gtgagattgg
     5821 ttcacctttt gtacaaactt tttctaaagt cggcattaca tttgctgcag gactgattaa
     5881 ttttgtcgtg attacagcgg cattatcagg ttgcaattca gggattttca gcgcaagtcg
     5941 tatggtctat acacttgcca taaatggaaa gatgtctaaa aaatttctga aactatctcg
     6001 tcacggtgcg cctttttatc ctgtcgtagc aatctcaggt ggaatcttaa ttggtctgat
     6061 cttaaattat cttctaccaa tgttcatcaa ccagtcagaa aatattttcg ttttcgtata
     6121 cagctcaagt attttgcctg gtatggttcc ttgggtagtg attttgatca gcgagattcg
     6181 cttcaaaaag aagcacgcag ataaaatgca tgatcatcct tttaaaatgc cactatctcc
     6241 ttatacgaat tatgtgacac ttgctttttt agctatcgta ttgattttca tgtttatcaa
     6301 tcctgaaacg agaatctcat tgattattgg actcgttttc ttgctttaca tgacgatcca
     6361 ttactttgta cgcgaaaaaa gaaccggaaa ataaaaaaag aacgaagctt caccttagtg
     6421 gaagcttcgt tcttttttat tgcttttatc aattcaattt tagcgtctca ccgggagtca
     6481 ggaaataatt ctccatgctc atcccattca gcgacaagat cgtttctact ggtacgccag
     6541 ttcgttcggc tatttgattt ggaccttctc cttccaaaac tacggttgtc gcagcctgtg
     6601 aagtcgcgct gttggtatta gtttcagagg aatctgttgt gtcttgtgtt gttcgagctt
     6661 gcatttcaga agcttctacc gactctgctg tctcagaatc agaagatgtt gtgtcttgtg
     6721 tgcttgttga ctctgcttga agagaggcgc tagtctgcaa ataaatatcg gaaatcttag
     6781 cgacatgatt cgtataatta gccgataatt gattgatcaa agtatctaag tctacaccgt
     6841 ctttattttc tgctgcctga tacgctgcac gtaattttct gatcccttcg tcggagattg
     6901 cttgaagctc acgtgctttt tggttagcaa ctgctgataa accagctatt ccctcttgat
     6961 ttccctgaat ttctgtctgg tattcttcaa tcaatgcagg cgtcttttct tcgatctttt
     7021 tcgaataatc atttacgatt gcttgaagtt gctctggcaa accatttgct gttttttctt
     7081 cagaagtttg tgtctctttt gttttagttg atgactcact tgtttgtttc gtctctgttt
     7141 tctttgtcgt tgtttccgtt atggtcgtac ttttcgcttc tgacgaattt tttccatgaa
     7201 aatatagacc cgcaactcct cctgctatga tcaaaaacac ggcaactgct gccccaatca
     7261 tgatcatccg cttacgagct gcctgttttt gctgtcttct tcgggtcatc cgaacatttt
     7321 ctccccactc attttcatca tacatttttt tcaccaccaa tttttatgta taatagcgtt
     7381 atcaaacgct tcatattctt attgtagcaa ataattgtcg ctgtgcacat ttttatcttt
     7441 gttatttata agaaaatttc accagttgag atgattccaa gattgtccgt cgatttctcc
     7501 ttgcagctgc attgttcgtt cgtctatctc agccatcagt ttctttattt catataagag
     7561 cgcacggtct tgatattctg atttttcgct taattcatct aattgttgga tcagccaatc
     7621 atttgtcata atgaagttcc tttcatttct tgcagtgtaa aggtcaagtc ttcgatctgc
     7681 tcttttgcct cagtcagtgc ttctacgata tatgcttgtt tttcagattc ttcttcctgc
     7741 attttctgat tttcctgtac tctttgaata aaccagtcaa tccgctgagt cagcgatggt
     7801 ttgccttcca atgagagata ctgctgataa gcaattagct tctcccaacg tttttcctgg
     7861 gataaataca taaattgtgc taaaacgaat ggcggaagat aggtcatatg gaatttattt
     7921 gcgattcgct gatacggcct caaaaattcg gaaatcgtat attcttcttt tcctccagct
     7981 tggtattctt tttccgccac acctgttgtc aatacgattc ctagttcttt cccttttaat
     8041 acatgtgctg ctcgttccaa gacattatct tgccaaattt ttaaatgagc aggagaacta
     8101 taccagtaaa acggaaactg gaaaatgatc cgatcatgct gggccaagag ctgtagttca
     8161 gtctctatat ctatttttcc gtctggataa cttgattcca aatgatgcca agtagctggt
     8221 aaaccagcag aagcttcttt gaaaaattgc tgatttaaag attcttcgat cgttggatgg
     8281 ctaatgatga taagtgtacg catccctttt cctccttttt agtcttcgct ttacagtgta
     8341 gcataaccgc cacaagtttc ttttgccgat gcttgtcatt ttaaccagac taatcaggtt
     8401 gtccaagaaa ctgcggtttg tttttccaac tgttttttca aggtatccaa agcaatattc
     8461 ttaaaatttt cgtttttcat acgtttacga tagttcgtag ccgtttctcc ggtactcttt
     8521 ttgaacattc ggctaaatac gactagacta ttgaacccta cttgataggc aatactttcc
     8581 acggattcat ccgtcagaga aagaagaaac gcccctcgat tgatccgtac cccgatcaaa
     8641 tattccttga tactggtacc catatgattt ttgaatactc gcgacaaatg gctccgattg
     8701 accgctaaat gctgggcgat ttcctcaaca gacaattttt cagcataatg tgcctcgatg
     8761 tatttgactg tttcaacagc taaagaggaa tgaacctttt gtacaccacc gctgaccttt
     8821 ccgccatctt ttaaaaggac tgataaaaaa cggtaggtta atgctgttaa ctgtaattca
     8881 ttttccaagg tttcttccgt atctttcatg cattctaaaa taatgtcgat atatttttgt
     8941 atgtcagaag agaccatcgt gtattggtct tctctgaaaa gcgactgccg aatgatttct
     9001 tctgcagcac tcccaccaaa tgaaagccag caatacatcc acggatcttc tccatctgat
     9061 aagtaaaacg tcgagtctcc tggacgaatc aagaacagat cacctttttt caattggaat
     9121 tgttgatttt tcacatgata agatcctttg ccttccatca cgacatgcag gatgtaatca
     9181 ttgcgaatag caggaccaaa agaatgaaac ggcatcgttt tagagaaacc acaaaaatca
     9241 aaatacaatt ctttttctac ttgttgatgt tcctttttta aaaaaatcga ttgctccacg
     9301 tctatccctc cttactcctt tagtataatg caacaaaaag ttaaataaaa gtcacagaaa
     9361 tatattaaaa aggaagcgat ttcttgttaa gtttaagtca ttaaaagatc tttgttattc
     9421 gttgtaaccg gtttacttag tattatatat tgaacaggag aggaatacag attatgaaaa
     9481 gcaaaatgat ccaacgtgtg tcctacgctt taggcgcttt tggacacgac acattctatg
     9541 ttacattaag cacttatttt atggtattcg tgacaagcca gatgttcgca ggaacagata
     9601 aggcaacgaa tgcaaaaatg attgctacag ttacaagctt agttgtaggt atccgcttag
     9661 ttgaaattat ttttgatcct ttgattggtg gcatcatcga taatactcgt acaaaatacg
     9721 gtaaattcaa accatggtta gttatcggcg gcgttgttag ttccgtactc ttggtagcaa
     9781 tctttaccaa tttctttggc ttagctactc ataatacgac tttatttatc atattatttg
     9841 caatcgtttt catcttattg gatagttttt attcatttaa ggatatcgct ttttggtcaa
     9901 tgattccagc tttgagtacg gaaacaaagg aacgtgaaaa atttgctacg tttgctcgtt
     9961 tcggctcttc tctaggtgca aatggtacta ctctagtcgt tgtccctatc gttactttct
    10021 ttacctatct tgttactcat aaacatattg aaggcgcttc aggctggttc tggttcggtg
    10081 tgattgctgc tgttatttca ggcggtactg cgattatcac tgccttgggc acaaaagaag
    10141 tcgatactgc gatccgtcag caaaatgaaa aagctgggat caaagatgtg ttcaaagcag
    10201 ttgctcaaaa tgatcagttg atgtggcttg cccttgctta tatcgcttat gcgattgcca
    10261 atgcagcaac aactgccgta ttattctact tcttcaaata tgtacttggc caaccaactg
    10321 aattttggat cgttggtcta gttgctgcct tacttggttt agtagctgtt ccactattcc
    10381 caatgcttac acgcgtcatc tctcgtcgct acgtttacct tggagggatc gcaatgatga
    10441 tttcttctta ctttttcttt acgatcgcag gttcaagttt gccaatcgtt atcatcgggc
    10501 tagtattgtt ctacttccct caacaattga ttttcttatc cgctttgatg acgatcacag
    10561 actctgttga atatggtcaa tggaaaaatg gtatccgtaa cgaagctgtt actctatcaa
    10621 ttcgcccttt actagataaa atcgctggtg ctttgtctaa cgggatcgta gggtttgtcg
    10681 ctatcgcttc tgggatgact ggtaatgcta ctgctgctga tatcactgct catggcgtaa
    10741 cgactttcaa agcttatgca ttctatgctc ctgctgcttt gatgattttc tccgctatta
    10801 tttttgcttg gaagattaca ttgacagaga aaaaacatgc acaaatcgtt gaggaattgg
    10861 aaaacaaatt ggctccagct gatacaatgg aagatgaact tcacgatgca gtagttcctt
    10921 cccattaaga aaggatcttg aaaatgaaga ttacagaaaa agattttggc caaggttatc
    10981 acttgataac cttggagaac aaaaacaagc tagcactttc tatctctgat ttaggtgcta
    11041 gaatcgtcag tttaaaatca aatgatcgag aattagtttt gggatttgac accgctgaag
    11101 aatatatcga aaaagatccg tatatcggtg cttctatcgg tcgcaccgca gggagaatcg
    11161 aaaatggccg cttttctctc aatggaaaaa cttaccaatt agccacagat ccaaaaactg
    11221 gacataatct gcatggtggt gctcctggat ttgaattaaa aaaatggtct tatgtcattt
    11281 tgaatggcga aaatgaagct tctgtcattt ttactactac aagtcccgac ggagaacatg
    11341 gttttccagg tacaatggat gtggaaatcc gctatacttt gacaaaagat aatatctggc
    11401 gagtgacctc tcgcggcacc agtgaccaag atactttatt caatccgaca aatcatgttt
    11461 acttcaactt gacgggagat gcttctcagt caatcgatca acatgagctt tggctcaata
    11521 gcgaagctta tgctcctttg cgaacagata gtattcctat aggggtaaaa gaaaatgctg
    11581 caggaagtgc tttcgatttt caaataccta aaaaattagc gtccgttttt gctagtgatt
    11641 tggatcaaaa aaatcttgtt gacggaatcg atcatccatt tttcttaaaa gaaaccggct
    11701 tgggtaaaga agccgctcgc ttgactagcc ccgataaaag aatccaagta ggtattgcta
    11761 cagatgcttc tagtgtggtt attttcactg caaatttcgg cacagaaaca ccagaaatgc
    11821 gcaaccgaaa actggcacat cacggtggca tcacatttga gactcaaaca gctccaggtg
    11881 cagagcgctt ctcagcattt ggttcgattc atttaaaagc cggatcagta tttgaaacag
    11941 tgacagaatt caaaatcaaa acaagaaagg aataactcat catggaaatc aaaagaagct
    12001 taaacacaaa atttgccgaa acattcggac ctaaaaaaac tacacaatac ttctcaccag
    12061 gacgcatcaa tttgattggt gaacacacag attacaatgg aggccatgtc ttccccgctt
    12121 ccatcactta tggaacgcaa ggtgtagcag cacctcgaga cgataataaa atattagttt
    12181 attccacaaa ctttgacgat atcggtgtca ttagttttac tttagatgag ctagaatatg
    12241 ataaaaaagc aaactgggca aattacgtaa aaggaatggt ccttaaatta aaagaagctg
    12301 gacactcaat cgatcatggg tttgagttgt tagtggaagg aacaatccct aatggtgcag
    12361 gactttccag tagcgcttca ttagaattat tagtaggtgt tgtattagaa gatctgtttg
    12421 atttaaaaac agatcgcttg gaactagtcc aaacaggtaa aaaagtagaa aatgaattca
    12481 tcggtgtcaa ttcaggtatc atggatcagt ttgccatcgg cttcggtgag ctagatcatg
    12541 caatcttatt agatacaaat acgctgaaat acgaaatggt tccagtgaaa ctagatggtt
    12601 atgcaatcgt catcatgaat acgaataagc ggcgggaatt ggcagattca aaatacaatg
    12661 aacgccgtag cgaatgcgaa gaagccttgg cacgtttgca aaaaactttg cctatctctt
    12721 ctttaggaga cttagatgaa gaagaatttg aatcaactat tgaccaaatc ggcgatgata
    12781 cattgatacg cagagctcgc catgccgttt ctgaaaatca gcggactttg aaggcaaaag
    12841 ctgaattaga agctgggaac ttagaagcat ttggtcaact gttaaatgat tctcaccact
    12901 ctcttcgcta tgattatgaa gtaactggga tcgaattaga tacactagta gatgctgctc
    12961 aaaaacaaga aggtgtctta ggtgcacgaa tgacaggagc tggatttggt ggctgtgcga
    13021 ttgctcttgt caaagaagaa aatatacctg ccttcaaaaa caatgtctat gatgaataca
    13081 tgaaagttgt cggctatgct ccggaatttt atgttgcaca cattggaaat gggacaacaa
    13141 agatcgatcc agaagctgtt catgtttgat aaaaaaatca acgatctcag ctaaaaacaa
    13201 attgataaaa ataacctctc agcaatttcc aaatgtgagc cttttgctcg tatttgaaaa
    13261 ttgctgtttt ttattgacac ggaaagagaa cgtgtgttct aattgtgttg aagaggtgat
    13321 tgttatgtgg gcgaatcaaa atccggtatt tgtttatgaa aaagaacctt caagagatat
    13381 tttatgtatt gactgcaaat ctttttacgc ctccgtagag tgcgttgaaa aggatcttga
    13441 tccccttaca actaaattag tagtcatgtc ctatccgtcc gattcgacag aaacacgggg
    13501 tagcggatta atcttggctt ccagtcctgc agcaaaaaaa gcttacggca tcaccaatat
    13561 cagccgtgcc agagatcttc ccttccccta tccaagtgat ttatatattg ttgccccaag
    13621 gatggcctat tatatggaaa agaacagaga aatcaattct atctataaaa aatatgcaga
    13681 cgaagcgaac catcatgtct attccgtcga tgaaagcttc cttgatgtaa caaatagcat
    13741 ggcactcttt ggtaaaacaa atgcgtacga catggctaaa ctgatccata aagatgtgtt
    13801 tgagcagacc ggaatctaca cgactatagg aattggcgat aacccgctac ttgctaaact
    13861 agccttagac aatggctcaa aagacggaaa gcaaatgttt gctgaatggc gttatgaaga
    13921 tgtaccagaa aagttatgga aaatctcaga tatcactgac ttttggggaa tcggcaaacg
    13981 aacagcaaaa cgtctgaaac aaatgggtat acattctgtt tatgatttag ctcacgcgga
    14041 ttattatcac ttgaaaagca agctaggggt catcggtact cagctttatg cccatgcttg
    14101 gggcatcgat cgaagttttt tgggacaaga atatacacca aaatcaaaaa gtatcggaaa
    14161 tagtcaaatc ctggatagag attatacaag acgagaagaa atcgagatcg ttatcaaaga
    14221 aatggccgac caagtcggca ctcgcttgag acgatctgca gcaaaaacac aagtagtcag
    14281 cttatgggta ggatactctt taggctatct tgatccaaat ggcaagaccg ggtttcacca
    14341 gcaaatgaaa atcgaagcga caaatgctag cagagatcta gcagatgctt tattgaccat
    14401 tttccaccag cattaccact accaggatat tcggcgtgta ggcgtgaact gttccgacct
    14461 cgtatacgcg acaggtttac agcttaacct ttttgaagat cctgaaaagc agataaatga
    14521 agcgaaagtt gattttgttg tcgatacgat tagaaaaaaa tacggctttc aatcaatcat
    14581 ccatgcgcat agtctcttgc aaggaggacg cgccatcgca cgtagttctc tcgttggcgg
    14641 gcatgcaggt ggtatgagtg gattagaagg tggaaaagat gagacggaca gcaaaaaaat
    14701 tcattgacta taacgagtat cgagatcggc catttggatt aaaatggggg actgcttttg
    14761 cgatggaaga actagtcaac ggtatcaaaa aaaatgaaga agaagcacgc aaacagcctc
    14821 ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat actgacagaa gcctttttat
    14881 ctcgtaaagc agtaatcatt cagctcaatt taaaagatga atttggaaga ctcttggatg
    14941 aggtcgaagg gcacttcatt ggtgaagcct atgaagatta ctttatgatt gaccatcagc
    15001 cgatattgtg ggaagacgta cggtatattg agttaaagga aacaaaaaaa tggtttgtga
    15061 catgagaggt tgtgacagaa gtgatcaact tcaaaaaata agaaaggatt tttcgaaaat
    15121 tgcttcttaa atttttgtgg aattttggct tatttttcga agaagcaact tctggaacaa
    15181 cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt tttgagtaac aagccaaggc
    15241 gctgatttca caacctctgt ctatctatcc atctattcgg taagattcat gcatgacgtc
    15301 ttctttattt agtatgtaga gccctttttc ttctgaccat tcaacataaa tcagctgttt
    15361 tacatctgca tagccttctt tcttcaagtt ctccataagc catgcttcag tcaattgatt
    15421 ggactgaagc actttatgct gaatttgccc ttcatctaca agcaggatcg ataaagcttt
    15481 gtcttcatct gattttttca atacactcat ttgaccggca ttctctagga taacatgttt
    15541 ggcattttca agagaaaagc attcttgctg tcttaagatt gctcgcaact gctccatctc
    15601 gatatggtta tttgtcaact ctgtcatgtt gatgacgccg tctttgatta gaacagatgg
    15661 ctcgcctttg acccaacgat tgaccttttc atttttttgg acgatacgtt cgataccata
    15721 aatcattact gcccaaagcg caatggcaaa taggacatgc cctacatgga cattgtcatc
    15781 ataggttgat tcttctaaga tcccacctaa aacaagcgta tagactaaat caaacggtgt
    15841 gatttctgac atggatttct tcccaagcaa acgtaccact aataataaac cgatcaaccc
    15901 gaacaccagt ttcattgtaa ttgaaaaaat cattttttct ccccctaact atctgttaat
    15961 taaattatat atgctttctc acgaacacgc atggaatata caaggaagaa atgtatcctt
    16021 ttactaaaaa aaggactgtg acactgtagt gacccccaaa agttagactt ttttggagtg
    16081 gagaaatcca ctcctttttt ttatgctcta aaatgatatt tttacacaac cgttacggtt
    16141 gttttacgaa attggacgag actttgccag tttaatttct gttttatccg ttcattat
//
LOCUS       DACCQI010000070        16179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCQI010000070 DACCQI010000000
VERSION     DACCQI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.071"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..783
                     /locus_tag="IVO76_001813"
     CDS             <1..783
                     /locus_tag="IVO76_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7429771.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IVVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQPMEN"
     gene            1161..3203
                     /locus_tag="IVO76_001814"
     CDS             1161..3203
                     /locus_tag="IVO76_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7429772.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            3214..3834
                     /locus_tag="IVO76_001815"
     CDS             3214..3834
                     /locus_tag="IVO76_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7429773.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            3846..4304
                     /locus_tag="IVO76_001816"
     CDS             3846..4304
                     /locus_tag="IVO76_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7429774.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            4323..4601
                     /locus_tag="IVO76_001817"
     CDS             4323..4601
                     /locus_tag="IVO76_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7429775.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            4626..5993
                     /locus_tag="IVO76_001818"
     CDS             4626..5993
                     /locus_tag="IVO76_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7429776.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            6008..7153
                     /gene="dgoD"
                     /locus_tag="IVO76_001819"
     CDS             6008..7153
                     /gene="dgoD"
                     /locus_tag="IVO76_001819"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAP7429777.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            7180..7671
                     /locus_tag="IVO76_001820"
     CDS             7180..7671
                     /locus_tag="IVO76_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429778.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            8114..9313
                     /locus_tag="IVO76_001821"
     CDS             8114..9313
                     /locus_tag="IVO76_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7429779.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(9808..10566)
                     /locus_tag="IVO76_001822"
     CDS             complement(9808..10566)
                     /locus_tag="IVO76_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7429780.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            10734..11669
                     /gene="pfkB"
                     /locus_tag="IVO76_001823"
     CDS             10734..11669
                     /gene="pfkB"
                     /locus_tag="IVO76_001823"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7429781.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            11666..13111
                     /locus_tag="IVO76_001824"
     CDS             11666..13111
                     /locus_tag="IVO76_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7429782.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            13139..13594
                     /locus_tag="IVO76_001825"
     CDS             13139..13594
                     /locus_tag="IVO76_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7429783.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            13613..14587
                     /gene="lacD"
                     /locus_tag="IVO76_001826"
     CDS             13613..14587
                     /gene="lacD"
                     /locus_tag="IVO76_001826"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7429784.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(14698..15259)
                     /locus_tag="IVO76_001827"
                     /pseudo
     CDS             complement(14698..15259)
                     /locus_tag="IVO76_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            15403..16017
                     /locus_tag="IVO76_001828"
     CDS             15403..16017
                     /locus_tag="IVO76_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7429785.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
BASE COUNT         5058 a         2680 c         3439 g         5002 t
ORIGIN      
        1 atgactaaat catttacgga agaagtcact tcatttaagg aacgtgagct tcatgaccgt
       61 tatgccgcta tttatatgga cgcaacctat atccctttaa agcggaaaac cgtcgccaag
      121 gaagccattc atatcgtagt tggcattcgc ccggacggtt caaaggaagt gttgagctat
      181 gcgattgcac caactgaatc tatcacgatt tgggaagaga ttttaataga cctccaggaa
      241 cgtggcgtga aaaacgtcct ccttttcatc acggatggct taaaggggat ggcaggggcc
      301 gtgcagcggt tctaccccaa agcccgtttt caacattgct gtgtgcacgt ttcccgtaat
      361 atcagtcaca aagtgcgtgt cgatgatcgt aaggaagtct gtgatgattt taaaatggtg
      421 tatcaagcgt catctaaaaa ggcggcattg gaagcacgtg gtgcttttgc ggaaaaatgg
      481 aaaaccagtt atccaaaaat ggtggaatcg atcctttcga acgattacct gctcactttc
      541 tatgatttcc cattagccat acgcaagact atttactcta cgaacttgat tgaatccttt
      601 aataagcaaa tcaagaaata cagccaccgc aaggaacagt tccaaaatga agagtcgatg
      661 gaacgtttct tagtctcgtc ttttgatact tacaaccaaa aattcctagg tcgcagtcat
      721 aaaggtttcc aacaggccga aggcgagctc gaacaaatgc tgagtcaacc gatggagaat
      781 tagaaacaat ctacagtagg gaagaaccat ttacacaaaa ttattgacgc tcccaaaagc
      841 attgttttta gcagtttaat attattcttt ttcaattaga ctgacttgat ttagttacgg
      901 aatagatact ttaatgtgtt gaagaagcaa cgtcccgtgt ttatttacta aagcatccaa
      961 tattctaccg aatatttaat attcacatta gccctaaaat aattgtcata ccatgttaca
     1021 tttgatataa cagcgttcat tttaaaaata gctgttagtg tgacaaaaca ttaaaccaag
     1081 tgtgacaaaa aacgtgatga aagctttccg gaaacgtatg tgaacaagta tataatgatt
     1141 ttgtcaggag gatagtcaaa atgaataaac gaattcgtga aatactcaaa ctgattatta
     1201 aaaaccctga aatgaaactt tccactttaa caagtgaact tgatttgaca cgtcgtcaaa
     1261 tcaattacgc gatcaatcaa tttaatgaag aattgaaaac aaagaacatt ccgttaattc
     1321 agagaagtca ttctggaaat attacggtac caatcgaggt tatccaaatg atatcacagc
     1381 ctgattacga cataggtgat gatcaggaac ttgtcgttct cactgatagt gaaagacaag
     1441 cattgatagt gatgactctg atcgctaata tcgattacat cagtctggat catttggtag
     1501 atgcggttga tgttagtaag acgactgtta tggaagatat caaacgaacg gaaccgttca
     1561 tcagaaatta tagtctgaca attcgatata atcggatgaa tggttaccaa ttggttggtt
     1621 cggaacaccg tatcttacag ctgttatcgg atctagtaaa acggtttccg atttttcgcc
     1681 gagaaatgat tcgcagaaaa ttggcacaag atgtttctga agaagaaatc gtccatttga
     1741 tccataacat ggaacaaatg ttgcatctta gttattctga tgagtcggta gactatttgc
     1801 agacatcagc gagattttta gtaagccgtg ggatcaagat gtcagataag cacgcgtttt
     1861 ttcccgatga tgtgacggat actcctgaat tccgaatgct gatgatcttg gtacatgaaa
     1921 caggttggag tctatccaaa agttatatgg agtggttcac gttattattt ttaacttcca
     1981 atatttttga gagcaaaact actcaaaatt atgattcgga tcaagaattg cgaaaattga
     2041 tcagccaaat ggttgaaaat ttccaaaatc aaacgttgat tttgatcgat gaccgagaag
     2101 actttgaacg taggatactg agtcatctgc gtccagcctg ttttcgaata cgctataact
     2161 tgagtttagg agtttattca atagatagtc tgatcaagga tagtaatcat gcgattttga
     2221 ttgatttgat gaaagaattg attattccta ttgagaattg gctaaataag gcctttccaa
     2281 atgatgaatt agatctttta tcctattact ttggctatca attaagtaat cataataaat
     2341 tgaataagca acgacaaagg gctgtagttg tttgtactaa tggtgtcatg gtctcaaagt
     2401 tagtacgagc caatatggaa aaactttttc cagagatcca ttttttagct tccctatctg
     2461 tgcgtgattt ctacaaattt gaagctgatt atgatctggt ctttactacg acgccactaa
     2521 aaagttctat gctgcagttt atgatcgagc caatcatgac atatcagcaa caaatcagtt
     2581 tacgatatcg agttttgagt gatttaggaa ttactaaggt tgatcgagca atggatgaat
     2641 tgttaacaat cattaggaaa tatgcaaata tcaccaatcc agtttcgttg cgagaagaac
     2701 tgcagtactt tttgatcaag gaagatggaa gcacgccgct ggacaacttc aatgtattgc
     2761 cttcattgac ttattacttg cggccgagtt atgtacaaat cgttgaagaa gagatgtctt
     2821 gggaacgagc agtggaggtg gcttgccgac cgttattgga acatcaaatc attgactcgc
     2881 gttttatcgc tgattgcttg gatcaaatca gacaaccggg atatgcaggt tacttgggaa
     2941 tgaagacttg cattccccat acaacggtcg ataaaggtgt aatcagtgac ggtgtcagca
     3001 ttttagtttg taaaaagccg atacgatttc ctaatggtga agatgtcagc ttgattttgc
     3061 cgctttcatt ttttgattta acgaagcatc tgcgagccat caatcaaatc gctgatatcg
     3121 ccaaagatga ttcattgatt gagcagttat tgattgctga tgacgaaaag acagtttatc
     3181 aactattaag acaaagatct taaggaggaa aacatgagac tagcagatta tcctaaattg
     3241 acagtgatca tgcgtggtta tacttttgat caggcagatg ctattttaca agcaatgcag
     3301 gaatttgacc atcaatttgc cgtagagatg acaatgaaca caaaaggagc tcttgaaaat
     3361 atacatgaac taaatcaacg ttatggtgac acgacttata tcggcgctgg gacggtaagg
     3421 acattagaac aagcccaagc ctgctatgag gctggtgcaa aatttttatt aggaccacat
     3481 atgttcacaa aagaaatgtt gtcttacgcc aataagaaag gattattggc ggtacctgcc
     3541 gcaatgactc cttcagaagt cgaccgtatg tttaaagaag gagctgatat tgtaaaagta
     3601 tttcctgcag cggttgttac tccacgtttt ttcaaggata ttcaagcacc tttaggtaag
     3661 ttaccactaa tgggggttgg tggaatcagt aaggagaatg cgaaagagtt ttttgaacaa
     3721 ggtgctagct atttaggtct tggttccggt atgttcaaca agcaagacat cgaagagcta
     3781 aatgttaaaa acttagcacg ttcgatgaaa gaattaattg aggctattaa ataggagtga
     3841 ggactttgga actttatttg aatgaggaac tcgtttttcg aaatgttcaa gcaaaaacag
     3901 atagtgaagt cttagcgttt ttagcaagcg agatgtacaa aaaaaactac gtgaaggagg
     3961 aatatattca agcaatccaa gaacgggaaa aagaataccc tactggatta ccatcaacac
     4021 caccaggaat cgcaattccc catgcaaact atgaaatggt caataaaaca actttagcaa
     4081 ttgcgactct taaagatccg gtcctatttc ataatatgga agataacaat gagcagttac
     4141 caatccaaat tgtgatcatg atggcgattg gggaaccaca tgggcaagtt gaaatgttac
     4201 aaaaaattgt tggaatcata caagatgagc cactgcggca agaaatgatc agagcaggca
     4261 atgatactga attattagaa ctattaaaga aagcggtttt ttaatataag gaggaaatca
     4321 taatgaaacg aattttagta gcatgcggaa atggtatcgc cacttcaaca gtagtagcaa
     4381 caaaagttag agagtatttg caacaacatg gcattgatgt ttcaacgact caaacaaaat
     4441 tgatggaagt tccaggaaaa gttcaagact atgatctatt ggtgacgact ggtcaatttg
     4501 atggtcaaac aggtggtgta ccagtaatca aagggatgcc gattttaaca ggtatcggtg
     4561 ccgatcagac aatggaagag attttgaact tactgaaata atagctgatc aaaggagctg
     4621 aaaaaatgaa aatcattatg aacgtcttta acttctttat tgatgctgga ccaacagtta
     4681 tgttgccagt catcatcacg attattggtt tgatttttgg gttaaagatt tctcgtgctt
     4741 ttaaatcagg tttgacatta ggtatcggtt ttgccgggat caagttgata ttggacttta
     4801 tgacgaccaa tgttggacca gctgccaagg cgatggttga tcgaactggt gttaaactag
     4861 atgctttgga tgttggctgg ggttcgattg cggctgttac ttgggcatcg ccaatcattc
     4921 caatcttgat ttttgccatt ctattggtca atatcgtttt gctgattttg aaacgtacac
     4981 atacgctgga tgttgatatc tggaactatc accatatggc aatcgttggt gttatagtgt
     5041 attttgtgac taagaatgtc ttcttaggtg taggtgcatc agtagtgatg gcaattgcga
     5101 cttttaagat ttcagactgg tcgcaaccga tggtagaaag tttcttcgga atccctggtg
     5161 tttctttacc aactgtttct gcgctttcat cactggttat tgcttggccg ttaaattggc
     5221 tgctggatcg tatcccgtta ttcagaaaat caaaattcac catcaaagat gcacaaaaat
     5281 atttgggatt ttttggtgat tctatgatca tggggttagt tattgggatg gtgattggtg
     5341 ttttagccgg ctatgatatc aaagcggttt tacaattagg tgtcagcatg tcagctgttt
     5401 tggttttgat tcctaagatg acttcattat ttatggaagg attgatgcca atttcagatg
     5461 ctgcacaaaa atggtcacaa aaaaaattca aaggtacacg tttatttatc ggattggatg
     5521 ctgcagttgt tgtaggtaat ccagatgtta tcacaacagc attgatcatt attccactaa
     5581 cgattgcatt agctttagta ttgccaggaa accgtgtgtt accattcgca gatttagcgg
     5641 ttgttccttt ccgcgtggca atggtcgttg ccttgactcg tggtaattta ttaaaaaata
     5701 tcattattgg cttagttgtg acagcttcat tgctatggtg tgggtcagct actgcgccaa
     5761 tgctgacagc aattgccaaa tcggttggta ttaacttagg aacttcttca atgttaatct
     5821 catcatttgc tgcgacttcc atgatccaaa gttatttggt atttatcgca tttgcttaca
     5881 aaccaatcat cggtattcca gtgttgttat tagcctttgc gggcgcatgg tattacttcg
     5941 atggtatcaa aaaagttaat catgaaatgg atgaaccatt gaaggcacaa tagtaggagg
     6001 gtttaaattg aaaatttcag atgtcacagt gtataaagta aaaccaagat ggatttttgt
     6061 taagatctcg actgacgaag gaatcgaagg ctggggagaa atgatctcag gaactaaaac
     6121 agaaacggtt gttgctggag cttatgagat aggaaaccgg ctgattggac gtaatccgtt
     6181 tgaaatcgag cgcctcttcc aagaaatgca tcgatctttc ttccgaggcg gtcctattaa
     6241 tggaaccatc gtttcgggtt tagaaatggc tctgtgggat atcaaaggaa aagcgtttaa
     6301 tgttccggtt tatgaattat tgggcggtgc agcacgtgat aaaatcaagg tttattcttg
     6361 gattggtgga gatcgaccaa gtgaagttgc agaacaagca caagatcggt ttgatcgggg
     6421 ctttacggcc gtcaagatga acgcgacaga agagctgcat tatatcgatt cctaccaaaa
     6481 agttgatgaa gtggtggaac gagttgcgtc tattcgcgaa cgttttggtg atgcaatgga
     6541 tattggtgtt gattttcatg gacgggtcca taaaccgatg gcaaaagtgc tagcaaaagc
     6601 tttggagcca ttccatccaa tgtttttgga agaagtcgtt ttacctgaaa atgaagaaca
     6661 ctttaaagaa gtagctgatt ctgtggctgt accattagct actggtgaac gcttgtacac
     6721 tcgttggcaa ttcaagaacc tctttaaaca aggtactgtt gatattatcc aaccagatgt
     6781 ggctctttgt gggggaatct tggaaacccg taaaatcgca gcgatggcgg aagcttacga
     6841 catggctgtt gcgcctcatg cgccatatgg tcctgttgca ctagcagcaa ctctgcaagt
     6901 agactcatgt acgccaaacg tctttatcca agaacaaagc ttagggatcc actataacaa
     6961 aggttttgat ctgttagatt ttgtgaagaa caaagaagtt ttccaatata aagacggcta
     7021 cgtagatcta ccttcaaaac caggtctggg cttggaaatg gatgaagaca aaatcaaaga
     7081 aattgcacaa gaaggtttga tttggaccaa tccgcaatgg aaaaattatg acggtacgat
     7141 cgctgagtgg tgataaatga aaataaagga tgatgaaaga tggcagaagc acttatgatc
     7201 acagtttttt taggactgct gattattttg caaataatcg atcaaaagaa aattatcgtg
     7261 tttgctcaga gtagtcctgt aaggaagagt ttgagtattt tactgacagt cgccatcctg
     7321 ttgattttct ggccggaaca tctgactgat cagattaaac tggttgcttt tgcttctgtc
     7381 attttgatca atggtttttt gaaggagggt ctagcaaaag attctgttgt tagatttgga
     7441 gtattgaacg gtaactttaa tcgatatcct caagtccaag tagaaaaact aggtgacgat
     7501 aaatcattta tttcatttta caaaagcaag aataattctt tttcaatgat ttttgataaa
     7561 gatcaaactg aaatgatgga tgtctttcaa gaacttgata tccattccaa aattacttta
     7621 gatcaaagtt cggaagaaaa aagagcagtt gtaaaaaaag ataatgaatg attctactac
     7681 cgaaattatg atttctttat cacgaataat gaaacttgtt ttaatgaatc atattgattg
     7741 aagtatacaa tgtatattaa gttttggaaa gaaatatatt ggtacaagat atagcaggaa
     7801 tcgattactc gatccctgct ttttttaact tcctttgcag caaaaaaagc tcgtaatcta
     7861 agagatgatg gatagttttt agaatatata tattttcttc aattccagaa aaaaatgacc
     7921 aagctagtat gctaaaaaag tacgatgctt ctatgatctt ttttccgtct ttattaacag
     7981 atgttttaat tgatcaacgc atattgtaat ctaccctaat ttccatagtt cttcaccact
     8041 tttaaaaata gtaaaacccc tacaagtatg ctatagtaaa ttgctaaaga aaaaccaata
     8101 aggtgggaga tgtgtgcgcg atcaaacaag cggaaaaatg attcgttttt attatacaag
     8161 tttgcatttt ctttattgga tgatgttttg ttctgtctat gggtttgcga cattgttttt
     8221 gatttatttc aagattgatc cagctaaaat cggagggatc ttagcattgg tcaatattgt
     8281 ttcaacggtg cttcagcctt ttgtcagtca agtgatcatc aagaaaagaa agatcccact
     8341 gatcaccgtg ctgaagacga tgatagcagg attaggagtg attctcttag ctggggtatt
     8401 gattccgaat ttgccgatca ttacttatat cttgggtggg attttagtag tttctatgca
     8461 atcatttctc aatgcgctgg catttgagta ttcaaatacc ggctacagga tcaactttgg
     8521 cttttcaaga gctggtggtt cattttctta tgctgtaact tcttttttac tgggacagct
     8581 gttagcgatt tattctgccg cgattttgcc aatcttggtt atagttgaag cagtgatatt
     8641 tttcgttatt ttgcatctgc tgccggatgt aaaaaatcaa gaagtttttc ctatgacaaa
     8701 agaacaggat cgctcagttt ctttgagaaa aaaatatcct ttcctgattt tgttattttt
     8761 aggtttcagt tttcttttta ctttccacac catgatcaac agctttttgg cacagatctt
     8821 tgaaaatatc cacggcggca gtaaagaagt tggcatcgca ttgatgatcg ccgctttatg
     8881 cgaattaccg ggaatgatct tttttgaaca gcttgtcaaa aagaaatcca gcaaattttg
     8941 gctgtctgtt gccagtattt catttgctgt cagaggattc atgatgctgc tggccagttc
     9001 gatcctgatg atggaaatga cgcatctcct acaagccttc agtttcgctt tgtttattcc
     9061 agcttcggct tatttgttca atcaatttct ggcggatgat gatcgtgtat ttggacaaac
     9121 gatgatcatc atcgccacaa ctcttggtgg ggtgatagga aatgtcttgg gcggcagtct
     9181 gctgcagcat ttcggtgtat gggagatgct gattttcggt actggttttg cggtgctagg
     9241 tgctttatta actattttgg gcattcgaaa aataccgaag acaggaaacg atagtgattc
     9301 tcttgttgag tagcataaaa atacttagat actagtttta tataaataac atatagtgat
     9361 tattggcgta attacaaaaa acgagaataa cttaagtaag catttctaaa taattgagaa
     9421 acctcatcta aaaagataag aaaatatccc taataaaact tctgtaacat tttagataac
     9481 tgattgtaat ctgaataata aactataagg agacggttag acggtctcct ttttttgcgt
     9541 attataattc tagaattgtt ggaaattgat aataattaac aagaaatatt agacaggaac
     9601 ttcgaactgc ttatcacatt cttattatta tatatttgcg taagtagtcc tggtagtctc
     9661 aatgcactat tctatcttga aactaagaag ttatagaagg agcaatgata gaaagcacga
     9721 gaatacaaat agcgattaaa tatttttcta gacaacctta gttaattcac acagatatat
     9781 tttaggtaca gtcttacaga tatagagtta gtgctgtgtg aatcctgatg ttcgcagtgc
     9841 ataggagtca agtaagattc tagccagtat agaaagtgga agtctggagc gtacctcata
     9901 atctgggaag aaggagcctt ctgatttaaa gccaacaaaa ggaatctcga tatgttccat
     9961 caaacttgaa tgacgattag cagtaagcaa aattgtcccg atctcagctt tataacagtt
    10021 ttcagctgcg gtagtcaatt cctttgtttc accgtttagt gaaacaaaga tcacgatgtc
    10081 atctttggat aattttctac taatgttttt tatgatattg ggatcagtat gcatctcaca
    10141 atatttaccg gtaagttgaa atttgaccat catttcctga gcaatcaatt ctgaaaaacc
    10201 tcgggcaaaa atcatgattc ttctagcagc cttgattcgt tgaatagcat cttcaatatt
    10261 cccgctttct accatattga tggtgcggat tacctcttgc tcatttttaa gaatagcagt
    10321 ctttattcct tcatcaactt tatcaagagc ggcaaaattg atatttgtat tttctttttc
    10381 ttttaacgaa tgtttaaaag acgtaaaccc ctcatatcct tttttcttca tagtacgaac
    10441 gacggttgct gttgaaacgt tggcatattc acttagtttt acaatggaat aggaggggat
    10501 ttcgagtatg tgactttgaa taaaatccca gagatatttc tccgattcac ttaattttgt
    10561 atccatattt tgcctccgat tagttaggat aattctattg taaagcggga gtttccttta
    10621 cttcaagttt atttaatata ttttgtaaaa gttttcaatg aatttagtca tttatgaaaa
    10681 catttacatt cgatcttgat acataatgaa tatgtaagaa aggaggagcg taaatgtcag
    10741 tttatacatg tactttaaac ttggcaatcg acctttttat cgaaacagat gaaatgaaac
    10801 catcccaagt caatcgaaca aacgatgatg atatacaagc taatggaaaa ggtgtgaatg
    10861 tttcattgat catgaagcaa ttaggtatcg atagtacagc tttaggattt aaagcaggtt
    10921 ttaccgggaa ttatattgag gattatttgc atcaaaaaga tattagcact gattttatta
    10981 gcgtaccggg aatgactaga atcaacgttt ttacacaagt taataaaacc aatgaagaat
    11041 tcaaattggt taataaagga cctgagattc ctcaggaaca aatcaaaact tttctaaatc
    11101 aaatacaaaa gctgcaagct gacgattatt tgtgtgtttc tggcagtttg ccgcgagggg
    11161 tctcacccga aattcttatt gatatcagtc gtatttgttt tgaaaataag gtccatctga
    11221 ttattgattc aagctatccg gaaataattg attgtctgcc gtatcagccg ttcttattaa
    11281 aaccaaacga ggaggaattg caatcttggt ataatgtcga gcttagtgat cgtgaaggat
    11341 atattcatta tgctgaacgc ttgattgccg atggagcgaa aaatgttctc ctttctctag
    11401 gaggagaggg aggaatattt ttaaataagg atatcgttta ttctgggaat tctcctaaag
    11461 gtaaagtagt caatacagcg tgtgctggtg acactttgtt gggaacattt cttgcaggat
    11521 atttaaaagg tgaacaggta gatattaatt tgaagaaaag tttagcagct ggaagttcaa
    11581 ccgcttttcg taaaggatta acagattttt cggatgttgc agagttggaa aaacaaatcg
    11641 aaatcaaaga gggagcgagt aaaaaatgac aacttacaaa attgttgccg caacaggatg
    11701 tccaacagga attgcccaca cttatatggc tcaagaagca ttggagcagg cagctaagcg
    11761 caaaggggta tctatcaaag ttgaaaccca tggtcaagta gggatagaga atgaattaac
    11821 accagaagaa ataaaaaacg ctgacgctgt aatcattgca gctgacaaag atgttcaagc
    11881 agaacgtttc gcaggtaagc gtgtaatcga tgtgcctgtc agcaagggga ttaaagatgc
    11941 tgaccaattg atcgaagatg cgttggctgg taaaggttca atcactgcag aaaacaaaac
    12001 agttgacgct gctggtgaga aagcggagat gggggttgca gcaggcattg gtcacagtat
    12061 ttataaaaat ctcatgaacg gtgtttctca tatgctgccg tttgtagtgg caggtggggt
    12121 aattattgca atttcgtttg ccatctgggg tgtgtactct tttgatccca atgatcctac
    12181 ttacaatccg actgccgcta tgttaaaggg tgttggtgat gcggcaatgg gaatgatggt
    12241 tcctatttta gcagcttata tcgctgaagg aatcgctaaa cgtcctggtt tggttgttgg
    12301 gtttgttggc ggactgttag ccaattctgg cggagccggt ttcttaggcg gaatttttgc
    12361 aggtttctta gcaggctact ttatcttact attgcaaaag gtcatgaaag gcttgcctaa
    12421 acaattggat ggtctgaagg cgatcttctt atatccggtg atcggtgtct tgtttgttgg
    12481 tgtagtcatg tctttattag caaatccaat ggaagcagtc aaccaaggaa tgatgaattt
    12541 cttggcagga ttccaaaatt ctaatccatt aattttaggg attatcgttg gctgtatgtc
    12601 agcatttgat atgggaggtc cagtcaataa agcggcttat gttacaggga ctgcgttgtt
    12661 ggcacaaggt aaccatacat ttatggcagg ggtttcggca gcttgtatcg cgcccccatt
    12721 gattactgga tttgccgcac tgttttttgg aaaatatttc gacacgaatg atcgcaacgc
    12781 tggtttagtc aactttatct tgggctcaac gcacattacc gaaggagcaa ttccatttgc
    12841 ggctaaagat ccgctgcgtg ttctgccaac tatgatgcta ggttcatcaa ttgctgctgt
    12901 actgacatat atgtttggtg tccaagttcc cgcacctcat ggaggttttc tagtattacc
    12961 tgttgttact ggtaaggttg catgggtagg gtcgatttta cttggttcaa tcgttggcgg
    13021 agtaatttta gggttgatcc aaaaacgtag tgtagaaaaa cgtgaagcag aagcagctgc
    13081 tgttgaaatg aaagaagcaa ccaacaacta aaggttattt tagaaagaag gatttcaaat
    13141 gactttgatc aaaacaaata aagtttttat gaataaaaat tttacgacgc aagatgaggt
    13201 aatgaacttc cttgcagatc agtcaattgt cttgagcatt agtgaagacc gagatgcagt
    13261 ttacgaaaaa ttgatggaaa gagaagaaga aagcacgact ggtatgatgg atggttttgc
    13321 catccctcat gccaaagatg ctagtatcaa ggatgccgat attgtgattg ttcggttaaa
    13381 cggtaaggtc gattggaaaa gtttagatgg agaaggaacg gattttgtaa tcgctctgtt
    13441 tattccagat tcagaggctg gaacaaccca tctaaaattt ttatctcagg tagccagact
    13501 tttgatgcac aaagatgtga cagaaggtct gaaacaagcc cagtctgctg aagaaatagc
    13561 tgaactttta aaccaaaaat tagaagaaaa ctaaaaagga agtagtgaaa tcatgccttt
    13621 aacagaaaaa aagaaaactt atattcgtca actaagtaat gaaagtggca tcatcagtgc
    13681 gttagcaatc gatcaacgtg gtgccttgaa aaaaatgatc ggtaaatttc aaaataaatc
    13741 agcaaccgat gaacaaatca tcaatttcaa aaaaatcatt tcttctgaat tgactccata
    13801 tgcttcagca atcttattag atccagaata tgggttgccg gcagccgaag ttcgcgataa
    13861 aaatgctggt ctgttgttgg cctatgaaaa aactggttac gatgcttcaa ctccaggacg
    13921 tttaccggat attttatcta tttggtctgt aaaacgtcta aaggagcaag gggccgatgc
    13981 ttgtaagttc ttgttatact acgatgtaga tgaatctgat gacatcaatg accaaaagaa
    14041 ggcgtttatg gagcggattg gttctgaatg tgttgctgaa gatttgccat ttttcttaga
    14101 attggtttct tatgatgcca acgatcctga tacttcttcg aaggaatatg ccttgaaaaa
    14161 accgcataaa gtaaatgaaa tgatgaagga attttctaag cctcgctatg gagttgacgt
    14221 tttgaaagtg gaagttcctg ttaatatgaa ctatgtggat ggttacacta aaaatgaagt
    14281 tgcttatacc aaagaagaag ctgctgctta tttcaaagaa caatccgaga ctaccaacct
    14341 gccatttatt ttcttgagcg caggagtaac tgctgaattg ttccaagaaa ccttgaaatt
    14401 tgcaaaagag gctggctcta cattcaatgg ggtactctgt gggcgcgcaa cttgggcgaa
    14461 tggtgtagaa ccattcgtta ctaaaggtga atatgctgcc aaagaatgga tgcagacaca
    14521 aggacgcaag aacatcgaag agctgaataa tgttttgaaa gaaacagcaa cagctattaa
    14581 attgtaagaa agctttaact ataaccttaa gtttgtaacc taatgatttt taataatcaa
    14641 tttgagcttt taaataaaga attggaagcg tcccgacgct tcctttttcg tttgttttta
    14701 aggcatgata ggttctatgt tgtcgttcaa taagcgcaag aaggtcagca tttttttctt
    14761 agagtatgca aaattgcttt tctacccaat gatttaaaac ggagaggcac ccttggatat
    14821 ggtagatggt gaagtaacgc atttcttctg tgatcacggt ttttttgtct tcttgtagcc
    14881 agatttttaa ataattttct tcgatcagat tatttaatct cttttgaaat tggatatctc
    14941 cgtggttgat gacgaataac ttgtatacga acgtattcgt atagacatag tcgatcaatt
    15001 gagcgtatag atcctcgtag ctatgtgtcg tatcgtagga aatgatttca tccaagtctt
    15061 ggaaaaagct attttccagt ttttcataaa gatcatagat atccgtataa tgtgtataaa
    15121 atgtcgaacg gtgaagatca gcagtttcgg tcagttcacg gatggtgatg ttttgaattt
    15181 ttttatctac taataatttt gataatgctt ctgctaacgc tttttctgtt tttttgacgc
    15241 ggcgatcctc ttttttcatt ttgcagcctc ctaatcaaca taatcaagga attgtgtcgg
    15301 ttatccgaca atttcaccgc tatctgaaca tagataaccg acaccttgtc tattataata
    15361 taagtatagc atatcaatat ttaaagaata ggagaataag tgatggaatt agcctttaag
    15421 caactggaaa agaaagacta caaaaaagcc attcaattcg cgattaaagg tatgcacttt
    15481 gattggtata ccaagaacaa actccttttg aatctttatg gaagatactt ttggtatcta
    15541 gaattgaatc gcgcgacaca aatcattgct ttatatgcgg atgatgagtt ggcaggtgtt
    15601 ttattagcag acatcaaagg aaaatcaaaa aagcatcatt catttagtca aaagttatat
    15661 gttaaactat ttgatttctt acaaaatgca tttgtgaaag acagcaaagg cgggtatgac
    15721 gacaccaatg acgaattgct ttcagactat ttaaaaaaac attccccaga tggagaaatt
    15781 gtctttttag ctgccaaccc tgatttaaag atcaagggga tcggtagtaa attattaaaa
    15841 gaatttgaac gccgagagca aggaaaagaa gtatttctgt tcacggatag cggctgtacg
    15901 tatcaatttt atgaacgacg cggtttcgaa cggaaaaaag aaaaagataa cgtcatgaag
    15961 atccttaata aagaagtaga tctccgctgt atgttatata gtaagatcat tcagtaatgt
    16021 tttttactat cagaaagcag aagtaccacg aaggtcaatg gaaaaagcga gtaattgatc
    16081 catttagatg aagataggtg tagtctcaaa gatattagct ggcaaagata tccccagtta
    16141 aaaatagatt tctgtatcca acaataaaaa tatttttgt
//
LOCUS       DACCQI010000071        16117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCQI010000071 DACCQI010000000
VERSION     DACCQI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.072"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..307)
                     /locus_tag="IVO76_001829"
     CDS             complement(<1..307)
                     /locus_tag="IVO76_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7429786.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     "
     gene            complement(300..1568)
                     /locus_tag="IVO76_001830"
     CDS             complement(300..1568)
                     /locus_tag="IVO76_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7429787.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            1820..2281
                     /gene="mscL"
                     /locus_tag="IVO76_001831"
     CDS             1820..2281
                     /gene="mscL"
                     /locus_tag="IVO76_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7429788.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(2340..2786)
                     /locus_tag="IVO76_001832"
     CDS             complement(2340..2786)
                     /locus_tag="IVO76_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7429789.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(2879..3124)
                     /locus_tag="IVO76_001833"
     CDS             complement(2879..3124)
                     /locus_tag="IVO76_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429790.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(3239..3817)
                     /locus_tag="IVO76_001834"
     CDS             complement(3239..3817)
                     /locus_tag="IVO76_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7429791.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(3894..4424)
                     /locus_tag="IVO76_001835"
     CDS             complement(3894..4424)
                     /locus_tag="IVO76_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7429792.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(4428..5288)
                     /gene="rlmB"
                     /locus_tag="IVO76_001836"
     CDS             complement(4428..5288)
                     /gene="rlmB"
                     /locus_tag="IVO76_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7429793.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(5281..5676)
                     /locus_tag="IVO76_001837"
     CDS             complement(5281..5676)
                     /locus_tag="IVO76_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7429794.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(5673..7085)
                     /locus_tag="IVO76_001838"
     CDS             complement(5673..7085)
                     /locus_tag="IVO76_001838"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7429795.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(7082..7618)
                     /gene="cysE"
                     /locus_tag="IVO76_001839"
     CDS             complement(7082..7618)
                     /gene="cysE"
                     /locus_tag="IVO76_001839"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7429796.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(7940..9400)
                     /locus_tag="IVO76_001840"
     CDS             complement(7940..9400)
                     /locus_tag="IVO76_001840"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7429797.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(9570..10727)
                     /locus_tag="IVO76_001841"
     CDS             complement(9570..10727)
                     /locus_tag="IVO76_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7429798.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(10761..12131)
                     /gene="radA"
                     /locus_tag="IVO76_001842"
     CDS             complement(10761..12131)
                     /gene="radA"
                     /locus_tag="IVO76_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7429799.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(12194..12721)
                     /locus_tag="IVO76_001843"
     CDS             complement(12194..12721)
                     /locus_tag="IVO76_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7429800.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            12951..13757
                     /gene="proB"
                     /locus_tag="IVO76_001844"
     CDS             12951..13757
                     /gene="proB"
                     /locus_tag="IVO76_001844"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7429801.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            13754..15001
                     /locus_tag="IVO76_001845"
     CDS             13754..15001
                     /locus_tag="IVO76_001845"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7429802.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            15077..15421
                     /locus_tag="IVO76_001846"
     CDS             15077..15421
                     /locus_tag="IVO76_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429803.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(15538..15864)
                     /locus_tag="IVO76_001847"
     CDS             complement(15538..15864)
                     /locus_tag="IVO76_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429804.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
BASE COUNT         4379 a         3482 c         2955 g         5301 t
ORIGIN      
        1 agctggtttt cgtaaaactt gtgaaagcat tggcggaagc tccttgtttc ccaaataatt
       61 gaaagacatc accatcttct ttttcaaaca acttatgttc taaaaaatgg gcaattccat
      121 ctggaacttt caccttttct ttttcaccat agggaataaa ttcgttatcg atcgaaccat
      181 aattcgtact gaacaaacca tatgtcttgt gatagtcatt tttaggcaac aaatacacgg
      241 tcaaaccgtt tggcagtact tcatgataaa gtgtttcatt gatttgctca tattctgttt
      301 tattcatttg catttcctcc tgctaaaaag aagattgcct gcaattcgat ttgctcagct
      361 acttgttgta tctctttgat cgttacgctc tccatacgtt tcaaccattc ttcatcagat
      421 aattttgttt ccggcagcca gctgtccaaa aaagaagctt cgattgccgc ttgcggagag
      481 tctagagaaa gcagatattg gtttctcagc attgcttttg tctgagacag ttccaactct
      541 gttatttcac catttcgcaa actttctaat tgctcgtgaa tcagatgaag tacttgattt
      601 cggttctttt catcgatacc ggtttggaca ctcatgaatc ctcggaacgt atccacgcta
      661 ctagaggcat aataagctag actttctttc tctctgacat tcatgaaaag tttagagtga
      721 ggaaaaccgc caaataaacc gttaaagacc attagcgcaa aacgttctgg ttcatcgtaa
      781 tacacatttg tctgataagc taggttcagc tttgcttgaa cgattggctc acgtacttgt
      841 cgttccttca cgatattcac ttgaggttgg gtataaaaca gatctggata aactgtttcc
      901 cgcgactcaa atggtaattg cccaactgct tcagctgctt tgtccggatc aacatcgccg
      961 acaacaaaaa tgtcgatttg atcatttgcc atcatttctt gatagtagga cgctagcgaa
     1021 ctagcagtga tcttgtccaa atcttcgact acgcccaacc ctgggatctt ttgttccggg
     1081 ctgttttcaa aataaagttc ttgcaaggca taggaagcaa aagtttgttt atcttccttc
     1141 aaactttcca aatatagacg cagattgttc ttttccaagt caaacgtcaa ctgatcaaac
     1201 tgatgatttt taatatttgg ataaaataaa acctctttta aaaaatctac cgcttgagaa
     1261 aaaaggtttg gatcattgac atattcacca ttcacaaaac tgatccctgc gtttagccaa
     1321 tgtaaatttc ctttttttct aactgataat ccaaagcttg cgccataaag ctctgcaagt
     1381 tgcgcactta actttgtctg atccggataa ttcaaactat ttgtttccaa catgctgctg
     1441 agaagtgaac gtttcgtgat gacttcttgt tgcagacgcg cagtaaaacg gataaaaatc
     1501 cggatcgttt tgtattgttt tgttggtaag acatgaagat tcacgccttc tgccaattga
     1561 tacaccatat ttatatgctc cttttcacag ttgattgccc gcttactctt ttaatgggtc
     1621 aacctgcctt ttttacttat tcctctgctt gaaatttata agttcaggca attcgccgtt
     1681 ttttctaaat gatgttacac tttatagtat agcatagatg ttttttatca aaagaaacag
     1741 acggctaaaa gcgtatattg tttttttatt ttttattttt cgatatacta aagacaaata
     1801 aaagaaggga gggtcattca tgatcaaaga attcaaagag ttcatcatgc gtggagatgt
     1861 cttggatctt gctgtcggcg ttgttattgg tagtgcgttt acaagcatcg taaaccaagt
     1921 ggtgcaaggc ttgatcacac cactaatagg ttggtttgtc gcaatgatca caggtacttc
     1981 tgatttagaa ggagcacttt caattcttga ctggtcgccg acaaaaggcg tcacatttgc
     2041 gtttggtaac gttattagtg ctatcattac atttctgatc actggttttg tcttattctt
     2101 agttgtcaaa gcggcaaacc gtgcgaagaa tttacgctcc aaagaagagc aagtggaagt
     2161 ggaagaaact cctactgcag aagattatct tagtgacata cgcgatcttc ttgccaaaca
     2221 agtggcagaa gacaatacac atccagtaga gcaaacaaat ctttccgata cttctaaata
     2281 ataaagaatc taaaaaaaag aaagccgatt ttcagtgtcg gctttctttt tttagattct
     2341 taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc caaatacaca
     2401 tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc ttgtgcgccg
     2461 atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc ttgaacaatc
     2521 tttcttccta aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc aaaatggata
     2581 ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt atcttgaaaa
     2641 aacgtatgcc aagcttgtcc atcaatgtca tcaacttctt gataaggaca ctgttgttcc
     2701 acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt tgacaattct
     2761 tcaaatttct ttacctgata ttccactaag caaacgcccc ctgctttttt attcttagca
     2821 taccgattcg cacacaaaaa agcaaacgaa ccttacgaat ctcaggctcg tttgcttttt
     2881 aaactgcttc agttaaaaat tcgatctcaa ctgtccgagt aagtacatct gaatagctgt
     2941 aagatactcg ttcaaaagaa ttttcttcag gatccaaatc aacaacgaat actgaaggat
     3001 aggtttctgt taaaacgccg cggcgttctg tttgacgctt tcttcctgtc tgagcaacca
     3061 acatgattgg gctgccgata cgaccttcta aatctttctt gattgaagct aaagttgttg
     3121 gcatacagtt cacctctact atcaactata ccacatattt ttaaaatttt caagtttacc
     3181 ataaaaaaaa aagaaatgca ataaaaataa ataagaagct cattaagatt tttctaaatt
     3241 attcctcatt ataaaatttc aagtgttttt ttattttgag ttttactctc ccgtaagcgc
     3301 tcattacttg cttgtgatcc attgctaaac attcagccat gtctacaaaa tcaacgcctt
     3361 gcatataata atgaaaaatt ttagcttcta tttttgaaaa ctggatttcg caattttcga
     3421 ttgcttcttt taataaaaga tgctgaatcg ctaaaggttc ttgcaatgtg atctctgcta
     3481 aaaagtctgg tccttctgtc tctatttttt ggtcgattga gacttcatct ttttgatcac
     3541 gacgcttcag cgcgttctgc ttcctcaaca gagaatagat ttgattgctt ataatgcttt
     3601 tataataact tccaaagcta acacctttgc ttttttcgta agttctcaat gcctgatata
     3661 aagcgatcct tgcttcttgt tcccaatcct cagaaacata atttcttaaa taataggtgt
     3721 ttttcaaacg tagtaaaagt ggaaaatatt tttgatacag ggcagcaaat gcttcttcgt
     3781 caccattacg tgcagcgcgc aataactcct cagacataaa ataattcctc cataaattcg
     3841 tttatggagg aatcttatca tcaatgattc atcgaactga ttagaacgat atgttattta
     3901 ttttttctca ttaaatcgtc caatttttgg gacaattttt ttaactgctc ttcattccat
     3961 ggagaattac gtcggaaatc aataaaatgc atatctttat tttcttgggc tattgttttt
     4021 tccgtttttt ttatcgtctt atataattca taagcagaag ttcgtaatgc tccttgggaa
     4081 aagatcatcc actgttctgc taaatcgctt gtcgccactg tcacttgtgt caaacggtca
     4141 ttcagctgac cagcgattct ctcgatataa ctgtcagccg tttcgtcttc tttcgtaaaa
     4201 acaacatcca actggtattt cttataattt tgttggatgc ccggaactag ttgggcatcg
     4261 aagacaacga tgacttccat tccttcatat ttcgcataat ttgataaacg atgtaaaaga
     4321 gcttctcttg catccgccat tttgtcctgc tttttaagct ggacaagttc cggccacgaa
     4381 ccaatcatgt tgtagccgtc tacaatcaac agctgttttt tcatggtcta ctccccctga
     4441 cgttgggaaa aagcctgata cataagcaaa ccagcagcaa cactggcatt taagctctgg
     4501 acgtgcccgg tcatcgggat cgtcagcaat tcatccattt cttttttcaa tcctggactc
     4561 attcctttgc cttcattacc aatgactaaa gcaattgatc ctttggcatt ccatttttgg
     4621 aagtttgtgc cggtcatatc tgtgccaaat acccaaaatc ctgtttcttt gagctgttgc
     4681 acagcttgta ccaaattcgt cacgcgtgca acagggacat gttcgactgc cccagtggaa
     4741 gtctttgtta cgataggtgt gattccaact gctcggtgtt ttggtatgat cacaccatcg
     4801 acgccacttg cgtctgctgt ccgtaaaatc gacccaaaat tgtgtggatc ttcgatgcta
     4861 tccaaaatca aaaagaaggg gtcttctttt ttctcttttg ttcgttcaat cagttggtca
     4921 agggataaat attcataagg tgtaatcgca agaacgactc cttgatgcac gccgtgatcg
     4981 ctcaaagttt ctaatttttg tttaggaacc catttgactg gcacagcttg ttcacgtgcg
     5041 atttctttca gttcttctac tttttctcct cgtgaatctt cttgaagaaa gagtttattc
     5101 cctcgccctt gttgtagcgc ttctactgcg gcatggtgcc caaagacgaa attatcttct
     5161 aatgcttctt cattttgatc agaaactgac tgatttcgtc cagtttgctt tttatttgaa
     5221 tatctatttt gttttttgtc ttcaaatcca cgtttcgatt gatgtttgtt tgttctttct
     5281 ttacccattt ttttcaccaa ctttctgaat acaccagcgg atcaattctt ccatccgctc
     5341 ggtttgtttt gtcaaatgca gatagcccat gagcgcttcg aaccctgttg cgacccgata
     5401 cgtcgtaaca tcagcatttt ttgcgctcgt atggctttta gcatttcttc ctctgcgata
     5461 ataaagttct tcttcttctg tcaaaagatg ttcttccagc atttgctgaa tcaaaaaggc
     5521 ttgtgcattc gcagaaacat aattcgttgc cgcacgatgc agcttattgg gttttgtttc
     5581 tccttgatca atcaggtggt cacgaacata gatttcgtaa atcgcatctc caacataagc
     5641 caaggctaaa ccgttcaatt gtgtgtaatc tctcatgctt ctcttctcca tcgggttcct
     5701 tgcggtgtat cttctaataa gatccctttt tctttcaaca aatcacgaat ttcgtcgctt
     5761 cggacaaaat cacgattgct tcttgcttga tttcgttctt cgatcaattg ttcaatctct
     5821 tcatctaata actcttctgc tgtaaataaa ataccgaaga tcgaaagcca ttgcgagaat
     5881 aaagcttctc cttgtgccaa gacagcggca gaaactttcg gctggtctag ataagtgttc
     5941 agccacttag caagctcata gaccactgtg atcccatttg ctgcattaaa atcatcgtcc
     6001 atttcttcga tgaatcgctg ttctaaattt tctaacaaag cgattttatc cgcatcatct
     6061 gccaaattct ctgaggcagt ttcttggcgg aatttcaaat tgtcaaacgt attcttcagt
     6121 cgttgtaaat tggtggctgc ttctttcatc gtcgtttcac tgtagcggat cggacgacgg
     6181 tattgtgtgg tcgccatgaa gaaacgcaag acttgtggat caacgttttt gatcatttca
     6241 tgtacagtga tgaaattccc tagagattta ctcatttttt catcgtcttc gccaatcgtt
     6301 acatagccat tgtgcatcca gtaattagcg aattttttcc ctgttttcgc ttcactttgt
     6361 gcgatttcat tttcatgatg aggaaattct aaatcttgcc cgccaccatg gatatcgatc
     6421 gtttctccta aatgttttgt tgccataacg gaacattcaa tatgccaacc gggacgtcct
     6481 ttgccccatg gtgaatccca agaaatctcg ccaggttttg cttgtttcca taaagcgaaa
     6541 tctaatgggt cttctttttg ctgctgttct tcccctgtac gctgacttgc tccgacttcc
     6601 agttcatcga tggattggtc acttaatttc ccataatttt caaatttacg ggtacggtaa
     6661 tacacatcac ctgaagattc ataagcatag cctttttcaa ccaacacttg aataaatgct
     6721 aaaatatctg gcatatgatc catgactctt ggatgcagtg tagcaggctc gacattcaac
     6781 gctttcgtat cttcttcaaa tgctcggatg aaacgatcag caacttctgg tgctgtaata
     6841 cctaattcgt ttgctgtacg gatgatttta tcgtctacat ccgtgaaatt cgatacgaaa
     6901 tcgactttat agcctcgata ttcaaaatag cgtcgaattg tatcgaaagc aatggtgcta
     6961 cgagcatttc cgatatggat ataattatac acagtcggtc cgcagacata cataccgaca
     7021 tgtccctttt gtatagggat gaattcttct ttttttctgg tcaatgtatt gtagattttt
     7081 atcattctga tctcctcact ttttctcctt tgattcgaac gacttttgct ggtactccta
     7141 ccgctgtagc gtcgtctgga acatctttca acacaacggc acctgcaccg attttggcgt
     7201 tttttccgat cgttaccggt cctaatatct gcgcattcgc agagagcatg gctccttgtt
     7261 taacagttgg gtgtctttta cccgtttctt ttcctgtgcc gcctaatgtt acgccatgga
     7321 ataaaatcac atcgtcctcg atttctgcgg tttcgccaat gacgattccc attccatggt
     7381 cgatgaacac gccagttcca atcgttgctc ctggatggat ctcaacacca gttaggaaac
     7441 gccagaattg agcattgatc ttcgccaata aaaagagccg gtgctgatat aaaaaatgag
     7501 agaaacgatg ccaaaacaag gcatgaaaac cagggtatgt caaaacgatc tcagctgtcg
     7561 aacgtgcagc cggatcgttt ctctttgctg catcaatcgc tcgggtaaac caactcatga
     7621 ctttccactc ctttcgtaag aattttatgt aggaaatcat cttcttataa agaaaagaag
     7681 cgccttttaa ataagtttcc ttatcaaaag acgctttccg cgtggttcca cttttcttca
     7741 taatcaatga ttatctctga cagctaacgc ctgttatacg ttcttggcta ctttgtttcc
     7801 tataaaacag tttttcctgt tttctgtgtt tcgccaagac cactcccaga ggcatttcgg
     7861 caaccattca ttgatacctt gcaccacccg gtatctctct tgtaatgatc agctgcttac
     7921 ttttttctgt tcatcgtgtt tagttcagta cttgatttaa atgtgctaat gatttttcac
     7981 gaccaagcaa ttcgatcgtt tcaggcaatt ctggaccatg catttgtcct gatacagcta
     8041 cacggattgg catgaaaaga ttttttcctt ttacgccagt ttctttttga acagctttga
     8101 tggcagcttt gacagttgga gcatctacta cttccatttg ttctagctgt gctttgaatg
     8161 cgtggagaac agtaggaact gtttcaccag caagtacttc ttttgctgct tcattcaata
     8221 caggatgttc attgaaaaat agcgtagaca gatcaacgat ttctgcagca tagctcattt
     8281 gaggttgata caggctaacg atcttcttca cccattcgat tgtcttttca tctgggtttt
     8341 ctgctacacg tccatcagcg attagataag gcagacacat gtcagtaaat gcatctagat
     8401 ccattgcttt gatgtaatgg ttgtttaccc attgcagttt tttcgcatcg aaagctgcgg
     8461 gtgatttact taggcgtttt ggatcgaaca ttttgatcaa ttcttggtgt tcgaagattt
     8521 cgtcttcgcc cacaggtgac cagcccaata atgcaataaa gttgaacatt gcatctggta
     8581 gatagcctaa ttcgcgatat tgttcgataa attgcaagat tgattcgtca cgtttgctta
     8641 gttttttacc tgtttcactg ttgatgatca atgtcatatg tccgaaggtt ggtggtgtcc
     8701 agccaaatgc ttcatagatc atcagttgtt ttggtgtatt ggcaatatgg tcatctccac
     8761 gtaaaacgtg agtgattttc atcaaatgat catcgaccgc aacagcaaaa ttataagttg
     8821 gcataccatc gcgtttttgt atgacaaagt cgccaccgat attatcggat tcgaacgaaa
     8881 tagcgccttt caccatatct gtaaatgcgt attctgtatt gcgaggcaca cggaaacgta
     8941 caacagattc tagaccttgt gcttcttttt ctgcttgttc ttcaggtgtc aagttcgcac
     9001 atgttcctgc gtaatgaggc atttctcctc ttgcgcgttg tgcttcccgt tctgcttcta
     9061 attcttcttc tgtgcaatag catttgtatg ctcgattgct agcaagcagc tgatcaatca
     9121 gcggttgata gatttcttta cgttcagatt gacggtaagg gccgtattct ccaggatttt
     9181 caggagattc atcccaattc attcccaacc aagcaaggtt ttccaactgg cttttttcgc
     9241 catcttcgat attacgtttt tggtcagtat cctcaatacg aataataaag tctccatcat
     9301 tgtggcgggc aaacaaataa ttgaataatg cagttcttgc atttccaata tgcaggtgcc
     9361 cagttggact tggtgcatag cgtacgcgta ctttcgtcat tttcttcctc ttctttcaaa
     9421 ctagactttg actaacagca aaaattccgt cagttacggc ttctcaaata tcatttattt
     9481 aaagccactc agcacaaatt ttaccttttc taaggtaaat agtaaagcca actggttgaa
     9541 tttccttacc agttggctga atttcttttt tattttgatt gtttagtctt tttctctgtc
     9601 tcttttttat tgtcgattcc tcgtcctgca tgtgcaggtt tagcaaagat catgcgtcca
     9661 gcagctgttt gaagggcact tgttacaacg acttcgatac gttcattcat gtaatgctgt
     9721 ccatcttcta cgacaaccat cgtgccgtca tcaaggtagg ctactccttg ttggcgttct
     9781 gtcccggctt ttactactag aacattcatc gtttcaccag ggatgacgac tggttttacg
     9841 gcatttgcca acgcattgat attaagaaca ggtacatttt ggaattcaga gactttattc
     9901 aagttatagt cattggttac aatgacacca tctaaaagct tagccaattt gatcaatttg
     9961 ctgtctactt cactgatatc ttcaaagtct ccatcgtaca tttcgacaga tataccctct
    10021 tctttttgca aggcatttag gatatctaat ccgcgacgac cacgcacacg tttcaaacta
    10081 tcccctgagt ccgcgatgta ttgtagttcg taaagaacaa aattagggat catcaaggtt
    10141 ccttctaaga atccagtctt tgtaatatca taaatccgac cgtcaatgat cacgcttgta
    10201 tccaagattt tatatttatg gaaattttct cctgattttc gttctaacag ttgactctca
    10261 acttgactag ctgtttcttg ctcgattttt ttgcttcgtg aaccaaaaac tttcttccat
    10321 tcttcgattc gtgtcgttcc cattctcaat cccagatagc ctaagatgat catcagtaaa
    10381 atcggcaata cactattgac gaatggaatt tccatattgt aaagaggggt cgagatcaca
    10441 acacctaaag tcaatccgat gatcgcaccg atcgcaccga agagaagata agtcaagctg
    10501 acctcactta gtttttgttc tacttgtttg ataatagctg agatatgttt ggccaacagc
    10561 aaagataata aaaagaaaat aagtgcgcct aacaggctgt tggtaaatgt attattcagc
    10621 catccgtttg actgttgacc gattgcctgc caagctgagg gtaaaaatga aatacccaag
    10681 cttgcgccga caatgatcat cagcaaagta atgacacgtt tttgcatcgt ttttcctccg
    10741 tttcgacaat catttattag ttagcggaac acacgcttca acgtttcagc caaagttgct
    10801 actccaacta tctcgattcc ttttggcgct tcccagttac ctaagttgtt tttaggtagg
    10861 tatacttttg tgaagccgag tttttggact tctttgactc gttgttcaat atgactgaca
    10921 cgtctgattt ctccagtcag tccaatttca ccgatgaaac attccgttgg ctgtgttcct
    10981 ttttctttat agcttgaagc aatactgaca gcgatagcta aatcaatcgc tggttcattt
    11041 aattttaccc cacctgcagc ttttaagtaa gcatcttgat tctgaagaag caacccagct
    11101 cttttttcca gcactgccat gatcagagat acccgattga aatcaagacc tgttgttgtt
    11161 cgtttggcat ttccgaacat agtaggtgtg actaatgctt ggatctctac caaaattgga
    11221 cgtgtccctt ccattgccac aacgatagca gagccagttg caccgtctag gcgttcttct
    11281 aagaaaatct gtgaaggatt agcgacttct tccaatccat gctcatgcat ttcaaaaatc
    11341 ccaatctcat tcgttgagcc aaatcggttt tttactgctc gcaaaatccg aaaactatga
    11401 tgttgttcac cttcaaaata taacaccgta tccaccatat gttctaacat tcttgggccg
    11461 gcgatcgagc cttctttggt aacatgaccg acgataaaaa tggctatccc atttgtttta
    11521 gcgattttga gtagttctgc agtggtttct cttacttggc tgacacttcc tgccacactt
    11581 gtgatgtctg gctgcgtcat cgtttggatg gaatcaatga taacataatc aggggagata
    11641 tgttcgatgg cacgactgat ttcgttcata tctgtttcag cgtatagata aaattctgtg
    11701 ttgatacttc cgaggcgttc cgcgcgtaat tttatctgct ctgcgctttc ttcgccggat
    11761 acatataaga cttttccgcc tatggctgct aattgctgag aaacttgtaa cagcaaagtg
    11821 gatttcccga tacctggatc gccaccaatc aagaccatag accctggcac cacacctccg
    11881 cctaacacac ggttcagttc ttctaatttt gttttgatcc gaggttcttt tttagggacg
    11941 acgtctgcga tttttgttgg ttttgctttt tctcctgtca aacttgtccg agttctgcgg
    12001 tccgttgtat cttgttcgat ttcttcgacc atcgtattcc attttccgca atttgggcag
    12061 cgtcccaaga acttaggcga cacatacccg caattttggc aaacaaattg tacttttgat
    12121 ttttttgcca ttgtaccgcc tccttttatc ctaatcatga gacattaaat caagcttaag
    12181 tatctttaac gttttattta ctggaagaac caaatccgcc ttgacgttga gcagtcgttt
    12241 gatcttgatc tgcttttaaa aacggcaaaa agatcccttg gccgatacgc tcgccttttt
    12301 tgatcactac atccttgaaa ccaaagttgg tgaattggaa catgatatgg ccttcatttg
    12361 cctcattatt gtaataatca ctgtcaatca cacctactcc gtttgtcaac actaaaaacc
    12421 gcttcaacgg attgcttgaa cggttagcaa gctgcaaaaa ttcatcttct cccatgtaag
    12481 ctttgattcc agtaggcacg agggttggtt tcaattgttt ttcattttct gatacatctt
    12541 ttttcaatcc atctgcgact gtttccaacc cctttttcca gcctgctttc cagatacttg
    12601 gaatcacgac ctcttctgct gcttcaaaat catagccagc agcatgatgt gtagcgcgct
    12661 gcggtagatt gatattttga tctttatatt tagaaataac ttcaaatcct ctttttctca
    12721 ccggttttcc tccctaaata aactactcct attctaacat atccgaggct gtaaaagact
    12781 ttctttacaa attcttcaag aaattctgct atcttaaagt aaaccattaa ttcgttcttt
    12841 ttccaaacga ctgcaagttg tacaaaaaaa ctatttttta catgacttta ctcgtcaaaa
    12901 caagtagaat agaacgaaat taatgagtgg atatttgatt gaggtgtcac atgcgtaaag
    12961 aaattaaaaa tgcaaaacga attattgtca aagtaggaac aagtacattg atttatccca
    13021 atggaaatat caatcttgcg gcaatcgatc agttagcttt tgtcctttct gatcttagaa
    13081 atcggggaaa ggaagtgatc ttagtctcat caggagctgt aggcgttggt atgcataaat
    13141 tacatttgtc agaacgtccg actttgattc ctgaacagca agctgcggcg gctgttggtc
    13201 aagccgaact gatgaatatc tataatcaaa gattcagtgt atataatcaa cagacggcac
    13261 agatcctcgt gacaagagac gtggtagatt atcctgaaag tcgtaaaaat gtgaggaata
    13321 cattggaaca gcttttgatt atggacacga tccctatcgt gaacgaaaac gatactgtag
    13381 cagtggatga attagatcat ctgacaaaat ttggcgataa tgatcagctg tcagccatca
    13441 tggcaaaact tgtcgcggca gaccttttga tcgtcctttc tgatatcgac gggttttatt
    13501 cagataatcc gactacaaat cccgaggctc agatgtatcg ctatatcgat aaaatcgatg
    13561 aaaagattct ggcacaagca ggtggtgctg gtagcacatt tggaacaggc ggtatgcaga
    13621 gtaaattaaa agccgcttct aggatatttg aagcaaacag cgcaatgatt ttagcaaatg
    13681 gaaaaaatcc ggcaattatt tttgatattc ttgcaggaaa agaagtaggt actttattta
    13741 aggaggactt gaaatgactg acttgatgca attaggacaa caggcaaaag atagcgcgca
    13801 acaactagca cttatgccta caaaccgcaa aaatgagcta cttgttcaaa tggcacaaac
    13861 catcaaaaag aatcaacaag cgatcattga agcaaatcat aaagacttag aaaaagcagt
    13921 cgaaaacaat atcagtgaaa cgatgcaaga ccgcttgcag ctgacacctg agcgaatcaa
    13981 tgccatggct acagagatcg aaaaaattgc ttcgttagac gatcctattg gaaaagtaga
    14041 tgagatgtgg acgaacaccg acggattgag aatcggcaaa aagcgcgtgc cactaggcgt
    14101 gaccggcatc atttacgaat ctcgtccaaa tgtcacgaca gatgcagcta gtctagcatt
    14161 caaatcaggg aatgccgtta tccttcgcgg tggcaaagaa gcattcttct cgaatcaatt
    14221 attagtccaa ttattgcagc aagtactggt cagtgctggc gaatctcctt atgccatcca
    14281 gtttgtcgat gatacttctc atgaaactgc aggacagttg atgcgattga cagaatactt
    14341 agatgtgctg attcctcgag gcggagcaaa attaatccaa cgagtcaaag aacaggcaac
    14401 ggtacctatc attgaaacag gtacaggaaa taatcacgta tatatcgata aagaagcaca
    14461 gttatctatg gcagtgaaca tcatcgtcaa tgcaaaagct tcccgtccat ctgtctgtaa
    14521 cgcggcagaa acattgctga tccattctga gatcgcacct tttttcttgc cagctattga
    14581 aaaagaactg gtagaacacg gggtctcttt acgtgcggat gctcgtgcgt tagaatatct
    14641 agagacggct gctttagcag aagacacaga ttgggatact gaatatctag attatatcct
    14701 tgctgtcaaa gtggtcgatt ctttggatga agcaattgca catatcaatc ggcataatac
    14761 taaacattca gaaacgatcg tgacagatag ttatgcggct agtcaacgtt tcttaaatga
    14821 agtggacgcg gcggctgtct atgtcaatgc ctccactcgt tttacagacg gatcggtatt
    14881 tggttttggt gcagagattg gtatctctac tcaaaagttg catgcccgtg gtccaatggg
    14941 gttaaatgaa ttaacttcca caaaatatat catttatggc gatggacaaa tccgcgaata
    15001 atgtttcgtg tgaaacatta ttcccgcctt ttgccttttt tctgtaaagg ttctatccaa
    15061 ctagaaaggt gtcaatatgt ttgaaatttt aatgattttt tttatttacc ttatttctct
    15121 gaatatcgct gcttttctgg gagtcggtat tttatccctt ttctttcaat tcaaaaaacg
    15181 aagcataggg agtcaacggg aaaaatggtc gcagtatttt gacaaaatcg ggcaaaaagg
    15241 gctggttaca cgcttgcaca ttagttacat ggttgcatta tgcttattag cgacaatcaa
    15301 ttactattct tttttcgatc attcaatcgc ttatacgatt acgctactga ttgctgggat
    15361 ttttcactta tcttataaat atcagctgaa taaaaaccac ttgaatcgga ctttccgcta
    15421 aaaaatatcc gtacgataag tagagtgcct tgaagatcgg caaaaaactg ccggactttt
    15481 tagaggctgt gacaaagatt ttgtcacagc ctctttttcc atataaacgg cgttcgccta
    15541 ccagctgcct ggtaaatctg tttcctcttc gttaagaagc gaaaatccgt caaatgtttc
    15601 gccatcataa taacgaagca gctcatccat ggacaagttc atcgtttttt ctttttcttc
    15661 ttccgctgat tcagccagta gctgttcccg atacacctga tacataccag ctattttttg
    15721 ctgcatcgct tttctagttt caccttccgc attgattatt ggcaattctt tgatatagcc
    15781 acgataaatc aaccgatcat ctgaaaattc aatgggtaat tcaacgagtg tcaacccgac
    15841 ttcctgaaaa tattcttcca gcatctatta tccccccatt ctttttctta aatgaatttt
    15901 agcttaccta ctgaaaattt tccagtttca ttattttgag aaaaaaaggg aatgtgacaa
    15961 aaatcgtgtc acatcccctt tttccgaata aatggtgttc ttctgccaac tcctcaatca
    16021 taagtcacaa tattcaacaa acatgagaaa ctgttttttg aaattgttcc ttgtgattca
    16081 gagttaaacg acagtctcac aacctcttgg taaacgg
//
LOCUS       DACCQI010000072        15072 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCQI010000072 DACCQI010000000
VERSION     DACCQI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.073"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..831)
                     /locus_tag="IVO76_001848"
     CDS             complement(<1..831)
                     /locus_tag="IVO76_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7429805.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     V"
     gene            complement(860..1177)
                     /locus_tag="IVO76_001849"
     CDS             complement(860..1177)
                     /locus_tag="IVO76_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7429806.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LDA"
     gene            complement(1202..1522)
                     /locus_tag="IVO76_001850"
     CDS             complement(1202..1522)
                     /locus_tag="IVO76_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7429807.1"
                     /translation="MEKRTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQIESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(1835..3250)
                     /locus_tag="IVO76_001851"
     CDS             complement(1835..3250)
                     /locus_tag="IVO76_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7429808.1"
                     /translation="MKITIPENFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAKIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYHSGNYGGR
                     IGCILNPEMVYARSSSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCRKQSIYF
                     DPATEDLETVQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREHEEQYMDEKGIVQDDYRIEF
                     IGQHLRYLIKAAEEGANCEGYMLRAFTDCVSPMNAFKNRYGLVRIELNDERSRILKKS
                     ASWYQSLIDKRELSLPDEAYK"
     gene            complement(3267..4583)
                     /locus_tag="IVO76_001852"
     CDS             complement(3267..4583)
                     /locus_tag="IVO76_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7429809.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLVTGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIVVSILIYLPFVKVANRVENDVI"
     gene            complement(4733..5431)
                     /locus_tag="IVO76_001853"
     CDS             complement(4733..5431)
                     /locus_tag="IVO76_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP7429810.1"
                     /translation="MEENKTLVEVTTDKILRYLKEKQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQISRMKSLTQKIETSFREGTQDHVQLDIDFHTTIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMATN
                     RRELQKIIGKKETN"
     gene            complement(5708..6697)
                     /locus_tag="IVO76_001854"
     CDS             complement(5708..6697)
                     /locus_tag="IVO76_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP7429811.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRELLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELKIDQILE"
     gene            complement(6804..7607)
                     /gene="msrA"
                     /locus_tag="IVO76_001855"
     CDS             complement(6804..7607)
                     /gene="msrA"
                     /locus_tag="IVO76_001855"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7429812.1"
                     /translation="MDKEQVFIQTLYNLILNPNTRDWERKVLIQTKNDIRENTSAKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLLTEGFDTQQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGNLSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SDLLTIYWQITDPTDAFGQFQDRGKQYRPVIFYQDERQKKLAEQSKQELVSSGTFRQP
                     IVTKIEPVGTFWPAENYHQQFYKKQPKRYKKIQQARKQFLIYQRIKNKW"
     gene            complement(7842..8585)
                     /gene="truA"
                     /locus_tag="IVO76_001856"
     CDS             complement(7842..8585)
                     /gene="truA"
                     /locus_tag="IVO76_001856"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7429813.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNSEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHA
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLMGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(8721..9458)
                     /locus_tag="IVO76_001857"
     CDS             complement(8721..9458)
                     /locus_tag="IVO76_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7429814.1"
                     /translation="MFKYMDKIKQLLDLVEKEEKKSIKEAIDVLTQANLQKRSVYIFG
                     ASHAGILAEEMYYRAGGMMTINAIFGREVMLDRSPITFTSQMERLEGYGTNLAKTVSF
                     KNQDVLILHSVSGRNPIIIDLALTAKAKGVKIISLTNVQYSRSVTSRHSSGKRLFEVS
                     DIVIDNHGDIGDACCELTGIVQKAGPSSTVIGAAILNTIIVEVCEQLIANGIKHPPIF
                     YSANLDGGDLRNAELYEEYKEVIHYEL"
     gene            complement(9459..10709)
                     /locus_tag="IVO76_001858"
     CDS             complement(9459..10709)
                     /locus_tag="IVO76_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7429815.1"
                     /translation="MNFISEHILKNPPVLLGLIAMLGLFIQRKSFSETVKGALTAAFG
                     MVALTAGVNMLVGTISPINIAVQTQLGVEVTEGLSDVTFTAEYGGTVGLAMFLGLVIH
                     LLIARFTPVKTIFLTGHMLWWFPFVFVAAGVEAGLSGGALIGISGILSACYWSFMPWI
                     MRKYVWDATGDDSFLIGHPTGVLSLVSGFVAKRVGNKEKSTEDIKVPENLSFFREISI
                     TGALVMFLMNTVVGIIAPVLIPEGDNLLMFSIEAGLNFGAGLLIMLYGVRLLINQIIP
                     AFQGIAEKVVPGAKPAFDVPILFNYRPNAVIIGFIVAMITSTILVVLANSFHLFGVLL
                     VPLVITSFFECGGAAVIGEGQGGFRGAVVGTIAASFVMVVLAGFSAIVFSTTIQSWIL
                     IFGGNDLSLWGMIGRGFSGLFGGF"
     gene            complement(10714..10995)
                     /locus_tag="IVO76_001859"
     CDS             complement(10714..10995)
                     /locus_tag="IVO76_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7429816.1"
                     /translation="MKILAVCGFGVGSSMVLKMSLDKVIKELGLKATVENTDLSTAKA
                     TMADVYFTSHELLPELKASVKAPVYPIKKYMDTSEIKAQLEQYLADKGE"
     gene            complement(11008..11439)
                     /locus_tag="IVO76_001860"
     CDS             complement(11008..11439)
                     /locus_tag="IVO76_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7429817.1"
                     /translation="MSTINKLLPKNQIQVIETAENWMKAVEIASNPLLKNKMITHTYL
                     ENMIRSVEENGPYMVLADYFALMHAKPGLGVNEQSMSLLVVKDAVDMKGKPIHIFLVL
                     AAKNHESHLERLKDIMEIFMDNEKYQTILSGNKETIIQLFA"
     gene            complement(11436..13076)
                     /locus_tag="IVO76_001861"
     CDS             complement(11436..13076)
                     /locus_tag="IVO76_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7429818.1"
                     /translation="MSVIQYQESKLRRVAILNRLSNFEHVSYQQLSEEYYVSRSSIAN
                     DISYIKYVFAKEGLNLSFDRFGTYFEGNEIQVQKVLKRMILNHFDELEIVREIVDRKL
                     LAIVERSFRESIADKQIELPESYFSSIIVSILLMIQRSREGKKISLKGKNQYGKYFLE
                     FNKYPLVCELLHQLEEQRIYQFSQEEVQYLTYVIVGSGLRFFMKNETIPLVFREKIRQ
                     LIQKVSRGIQIDLTQDSRLEEDLLVHLYQLLLRIEAQTTIVNPLIDEIKQNYPSLYGV
                     VWFALNDFRWPYQVSLSEDEVGFVAIHFQAAIERVKRLNRLLFVCPNGMGTSSFVSAK
                     IRRILPDIDSIETASVDKLNRLDLFDVDLIISTVDIPKQIRPVVRISPMVTARDMKRI
                     MNHYIDLVIDNEQINIEGSIAEKVKPLISSNVYFDHFDSKEAAIRFLVDQQQFENEES
                     KQRFLYSIFEREEIQSTYLENGFAIPHGNPNVVKQTSVAVLIADKPFSWGYQKADIVV
                     LLMIREEDVKEVEPVMKMVMQGIEDKNWFISKMLEVRE"
     gene            complement(13296..13931)
                     /locus_tag="IVO76_001862"
     CDS             complement(13296..13931)
                     /locus_tag="IVO76_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7429819.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EISSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTASIGFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWSFLNKIGFCQRISFFFNYVPIAWLSILHLRQ"
     gene            complement(14083..>15072)
                     /gene="sstT"
                     /locus_tag="IVO76_001863"
     CDS             complement(14083..>15072)
                     /gene="sstT"
                     /locus_tag="IVO76_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7429820.1"
                     /translation="LSALVAVIISSIFTVPITLQETVSEQAPQNLESVLSTMLTNVTQ
                     NPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQISVALSQVVQVIIAFAPIGI
                     LGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYPFLTFLFIKENPYPLILFCL
                     KESALPAFFTRSSAANIPINMMLAEKLKLTKESYSISIPLGATINMGGAAITISVMSL
                     TAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLLIPLACSLFGISNDVAMQIV
                     GIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGEKIKLKDRLDGLNER"
BASE COUNT         4559 a         3277 c         2458 g         4778 t
ORIGIN      
        1 gacaaagctg tcgaagaaag gtttgttcac gataaatggt gcgctatgtc cagctgcgat
       61 cgcattgaca tttgcttcga ttgccggcgc attgatcgtt tgcatcaaag gatcgaccat
      121 attcgccccg tgtaaaccaa agaaccataa gaatggtgtg ataaatgcaa gcaataatgc
      181 agatggataa gagttcgcaa gtcccataaa tggttcttgg accactgtat agaacgaacc
      241 tacgatatca tcaaccccaa atgcagcaaa aattgcagca actaaagcaa aggcagagat
      301 agtgatcatt gctggcagta atgaagcaaa tgctttcgca actgctggtg gcacaccatc
      361 aggcattttg atgatcaatt tttcatttcc gcttaaacgt acaaagattt ctgttgaaat
      421 aatcccaaca ataatagcaa taaacaaacc gtttgagctc atacctaatg ctccgcctaa
      481 agcaaagaaa gagcctaaag aaatcacacc tgcggaaaca ccgtcttttc ggtagccgtt
      541 agctaaatga tacgcaagta agaaagcaat caagacagaa agaatggcaa atgtgccgtt
      601 ccaagctgca gccccgaatc ctttccatga ttcaccgcca aaaattgaat tcatcaattc
      661 ttggaatccg ggaatcggta agttattgat cattactgct aatgctccta aaatcattaa
      721 cggcatactc accataaatg aatcccgaat cgcaactaaa tgccgttgtg caccaatttt
      781 tgatgcgaca ggtataaaat gtttctccat aaatgcaaca aatttatcca taaatcttcc
      841 ctcttttctt tactgtgaat tacgcgtcca accgacgata cagatcaatg atctcttttg
      901 ctaaatctgt aaaagcaata gatgtcatca ggtgatcttg actgtgaact gtcaaaagtg
      961 ttacttcaac gtggtttcct tgtgcttctt gcgtcagcat ctctgtttga gagtgatgtg
     1021 cttggactaa agattcctct gcatctttga ttttttcatc tgctaaagtg aaatctcctt
     1081 ttttcgcagc agcaatcgct tccattgcat cgctttttgc atttccgcca aacatgatca
     1141 gacccatgat ggcttctaga tttttttgat cttccatttc ttgtttcctc cgatattttt
     1201 attatttgtt tattagtttt tctgcttggt cgagtacttt ttcaccattc atcatgccat
     1261 agtcagccat attgatcaca tctagaggaa tgcctttagg tgctagcttt tcagcaaatt
     1321 gattttgcat aaaacgtact tgcggaccta agagtaaaac gtctacattt ttagattcta
     1381 tttgattatc tgcatctgag gcagagacag caaagatatc tgcttccatt ccttttgctt
     1441 ctgctgcttt ttgcattttt gtgactagga ggcttgtact cattcccgct gaacatacaa
     1501 gcatgattgt tctcttttcc attttaaaca cgttcctttc tattcttaca cttgtatata
     1561 aagcaagtag tgtgccaact ttaaaagcgc tttaaaaagt tgataatatc acgtttatgg
     1621 tctataccaa gttgcataca cactaaaaaa gaaagcgcac actaaacaca caaattcttt
     1681 tttattgtat ttatttcttt taacgtgcac aaaaaaagtt gtgacaagtc ttttgtcaca
     1741 acttttttga ataagaggtc aaaatgctac tttcacaacc tcctttattc caaataacca
     1801 tcaaatacaa tcaatatctt cgaaaaactt ctagttattt ataggcttca tcaggtaagc
     1861 ttaattctct cttatcaatc agagactgat accaggaagc agactttttc aatattctgc
     1921 tccgttcatc gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg
     1981 gtgaaacaca gtcagtgaat gcccgtagca tataaccttc gcaatttgca ccttcttctg
     2041 ctgctttgat caaataacgc aaatgctgtc cgataaattc gatacgatag tcatcttgca
     2101 cgattccttt ctcatccatg tactgttctt catgttctct tcccatacca ttttctgtta
     2161 tcagccaagg gatattatga taattttctt ttagatacat ggctatatcg tacatgattt
     2221 ttggatagat ctcccaccct ctagaattgt tcattttctt tccaggtaaa tcaaagactt
     2281 cataatatcg ttctggatgg aaaggtgtct cttcattcca ttgataacga ggagcacgaa
     2341 cgcgtttagg gtaatattga ttgatcccaa ggaaatctac tgtatgatgc tgaaccgttt
     2401 ctaaatcctc tgttgcagga tcgaaataaa tcgattgttt tcgacacaac tctaggaact
     2461 cttctgtgta gtaccctttt accatcggat caaaaaatac tcgattataa aacagatcat
     2521 acatataagc agctttttgg tcttctggag aactggaacg agcatagacc atctcaggat
     2581 ttaaaataca gccaatcctt ccgccataat tgccactatg gtaaatttct acagctttag
     2641 ccgttgctaa tactttgtga taattccaca tcatccattt ttttgtgttt tgttcatgcg
     2701 gccagcgaac agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa
     2761 accagttttt taccctgtca ccgtaacgat caaaagcaat ctttgcgtac ttagcataaa
     2821 aatcaaccac ttttcttgaa gaccagccat catatttttc caaaagaaaa gcagggactt
     2881 cgtagtgttc taaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt
     2941 cacttatatg ctgagcatat tcttcatcta ctatacaatt ctcgtaatca gtgaaaaaac
     3001 gagcccaatt gatagaagtc cgataatgtg tcagatggat ggatttcatc aaatctacat
     3061 cctgttggta ctgctccata aaattagttg ctacggctgg accataccca ttatgccaaa
     3121 caaaatgttc atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt
     3181 tccaaccttc cgtttgccaa gcagaagatg ctgcaccaag aataaaattt tctggaatag
     3241 taattttcat gatttcctcc tgttttttag atcacatcat tttccacacg atttgctact
     3301 tttacgaatg gcagatagat caaaatagaa acaacaatac aaacaagttg cgtaataact
     3361 gcaccgatat ttcctgctgt ggccagataa gcattgacga tcggcggcat cggccaagga
     3421 accattacaa ctgcttttgc cgcaaatcct aaagcggttg caaaataacc aatcgttcct
     3481 gtaacaagcg gcgttaagat aaacgggata gctaaaatag gattcagcat gatcggcata
     3541 ccaaaaataa ctggttcatt gatgttgaag atcccaggag caagagataa cttggtgatt
     3601 tgtttcatat cttctcgctt tcccactagc aatactgcta tcagtagacc tatcgtgacc
     3661 ccagacccgc ccatactcat atacatatcc caaaatggca tagtgataat gtttggtact
     3721 tcttttccag cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttct
     3781 cgaattggct tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaatg
     3841 aacatcaata acaaaatacc tggtagccct tgaacgacac cttctaatgg gcgttgtacg
     3901 acattgtaaa tgatatcata aaggtacatc cctgtaagtt ctttgatgat aaaccccgca
     3961 gccgcgatac ctgatactgt cagaatcgtg ggaaataacg cagaaaaact ggcagaaaca
     4021 ttcgttggca cggtatcagg cattctcagc tgtagttttt tctgtttcct caaccagcaa
     4081 taaagttcaa tggaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta
     4141 tattgtttag ccaatacgtt ctctactata acagtctgat tttcattcac taattcataa
     4201 acagttggat taacagataa ataagacatc actgcgataa taccagggaa tacaccatcc
     4261 actttattca gctttgctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa
     4321 cttagtgtga catagctgat ggcagatgcg ataggtttta attcttctaa aaaacgcaaa
     4381 aaacttatct gtgctaaccc attttccgaa tcaaagacca ttgctgaaaa caacgtgcca
     4441 aaagcacccg taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata
     4501 taccttaaat tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata
     4561 aaactcattc cagatcccct cattcctgct tcttctcctt gccgtcatct ctttttgcct
     4621 gttagtcagc gcttacattt cggtgaataa aaactatttg cttgtccgtt ttaccaaata
     4681 taaaacaaaa attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag
     4741 tttctttctt cccaattatt ttctgtaatt ctctccgatt tgttgccata tggacagtca
     4801 tcgcatcaaa tgccgcaagt acattccgcc tctcgatagc ctgaacgatt tctgtgtgga
     4861 tatcgatcgt ctcgattttc aaatgctgga tatcataatt tttgttaaat aaagcaatcg
     4921 attcattaat gatcggaatg atatgcagta acgctacgtt tccgctagct tctgcaatcg
     4981 tcgtatgaaa atcgatgtct aattgaacat gatcttgtgt accttctcga aaagacgttt
     5041 caatcttttg cgttaagctt ttcattcggc tgatttgatt tttcgttgca tattccgccg
     5101 ctaaccctgc catctttggc tccagtaaat atctcagctc aaacaagtct cgtatcatcc
     5161 ggtcctgatc ttcaatcaaa gaaaaaccga gtggatcatt agcaatccct cgcttatcac
     5221 tgacaaatgt ccccgcccct tgccgaactt caagaatatt cctagaagca agcgctcgga
     5281 cagcttctcg caccgtgctg cgtcccacct ctaaataatc ggacaactca tattcattcg
     5341 gcaatttatc tcctacttgc aactgctttt cctttaaata tctaaggatc ttgtccgtgg
     5401 ttacttctac tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat
     5461 tttttcgtaa ttattataac acgtttgaat catctgatga ttttttgtat aaaaatcgat
     5521 tcatccgttc atggatatac aaacgaagct atttaccata aaaaaagact aggataaaat
     5581 tcactcctag ccttcgatca tttttatttt gaacgacaaa acgtatgatg ttcaataata
     5641 gatgagcttc attcgattct tgatgacatt ttttgataga tggataaaaa gtttgattac
     5701 atcgtttcta ttctagaatt tggtcgatct tcaattcttc ttctggtgag aaatctaaat
     5761 tatccatcat tcgaacactt tcttgtaact gacttaaccg acttgctcca accagtacac
     5821 tcgtaacaga tttttgtcgc aaattccatg caagggccat ttgcgctaat gattgccctc
     5881 gtgatacggc gattgtctgc agtgctttga ttttttccag agttgatcca acttgttctt
     5941 ctgaaaggaa tggtatttcc ttacgatgtg ctctagaatc ttcaggaatc ccatgcagat
     6001 aacgatttgt cagcaaacct tgggcaagcg ggctgaacgt gattgctcct agaccttctt
     6061 cttctaatac tggagataag tcgtcttcta gtagttcacg agaaaacata ttgtagcgca
     6121 tctgatgaat aatgaacggt gcttcttttc tttttaaaat ttcagtcatt tttttggtat
     6181 cttctccatt atagttggat ataccaatat ataatgcttt cccttgtctt acaagttgca
     6241 tcaatgcttc tgctgtttct tccaaaggtg tatcagggtc tggacgatga tgatagaaga
     6301 tgtcgacata atctagcccc attctttgga gactctgatc acaacttgaa atgatagatt
     6361 tacgagagcc ccattctccg tatggacctt cccacatatg ataacctgct ttgctagaaa
     6421 taatcagttc atcacggtag gcttgaaaat cttctctaaa aatacgcccg aagtttttct
     6481 ctgcagctcc agcagggcct ccataattat tcgcaaggtc gaaatgggta attcccatgt
     6541 cgaatgcacc tcttaaaatc tctctttggt tatggatagg atcataatca ccaaaatttt
     6601 gccataatcc gagagataaa atcggtaatt tcaaacctga atgaccggat ttacgatatt
     6661 ccattttttc ataacgtgca ctatctgcaa tatacaagtt ttctcctcct taccattttt
     6721 aaaacgcttt ctttcgttct gcttttagtt tactacattt taaagaaaag gagttagtca
     6781 aaatgatttt ttcttgtatt tttttaccat ttgtttttta ttcgttgata gatcagaaat
     6841 tgtttacgtg cttgctgaat ttttttatag cgtttcggtt gttttttgta aaattgctga
     6901 tgataatttt ctgctggcca aaacgtacca actggttcaa tttttgtgac gattggttgg
     6961 cggaacgttc ctgaactgac tagttcctgt ttcgattgct ccgctaattt tttctgacgt
     7021 tcgtcttgat aaaaaatcac cggacggtac tgcttccctc tatcttgaaa ttgtccaaag
     7081 gcatctgtgg gatcagttat ttgccaatag attgtcaaca aatcgctata acttatctct
     7141 ctcgtatcat aaatgatctc cacagcttcc acatggcctg tgtaaccacc acttacttga
     7201 tcataatttg gtttttcaac atgtccgccc gtgtatcctg acaaaacgga taggttccct
     7261 tttttcgatt caaacggctc gaccatgcac cagaaacatc cccctgcaaa tactgcacgt
     7321 tcttgatagt cagcatcttg catagcatgc ttggaaaagt catatttttg ttgcgtgtca
     7381 aatccttccg taagcaataa ataaaagtcc attacgtcag gcgtcagatt cattcggata
     7441 gctaatggac gtagtgttgc ttctagtttt gataactgtt ctttagcgga tgtattctct
     7501 cttatgtcat tctttgtctg gatcaatact tttcgttccc aatctcgtgt attcggattt
     7561 aaaatcagat tataaagtgt ttgaatgaat acttgttcct tatccataga ctctcctcct
     7621 ttccgaagaa gacaagctag tgactttagc tgtaatttat cataaagtga ggaaataagc
     7681 gattgaatcg tatctattca aagaagataa gcggcttcag atgatataag atgatcttta
     7741 tcttaaaaag tatactctat ggtttggttt aactaaaaaa cgacctctga aaaaagggat
     7801 gttctccagt tctcagaagt cgagtaaaga tgattctttt tttatgaacg ataataaact
     7861 tcatccaaaa acagaccttg tgcagggacg gtttctcctg catgctgccg cactttgctt
     7921 tcgaatattt catcgattgt ttctatcggc atggcacctg taccgatttc tagtaatgtt
     7981 cccatgatga ttcgcaccat tttatataga aatccgtctc cgacaaaagt aaaatgaagc
     8041 atttctccct tacgttcgat agataattca tcaatcgtac gtaccgtaga tttttttgat
     8101 tttttcaatg cagaaaaacc aatgaaatca tgtgtaccca tcaatttttc gcaagctgca
     8161 ttcagcttat ccatatccaa ctcttgagga aaataaaagc tgtgatttcg ttcaaatgct
     8221 gaaggaatca cgttgttcca aacataatag ctgtattttt tgcccaccgc attatatctt
     8281 gcatggaagc gctcaggcat ctcttcgatt cttttgatga caatatctcg cggaagatag
     8341 cgattcataa agtcgatcat ttctgccagt ccaatttctg agtttgtttt gaaatttgcc
     8401 acttgtcctc gcgcatgcgt gccggcgtct gttcttcctg atccaataat ttctatctct
     8461 tcatttgtca tctgatgcaa cactttttcg attttccctt ggatggtttt atcgtcatct
     8521 ccaagtcttt gccagcctga atatcttttt ccgtcatatt cgatcgtcaa cttgatgttt
     8581 ctcatatttt tgtggctcct ctatattcca tttggtcttc tctctacctt tttaagattg
     8641 tagcacaaat taaagctact tgcttctctg tgagagaaac caagtagctt taaataattg
     8701 tttatgaaca attgtattat ttataattca taatgaatca cttctttata ttcctcatat
     8761 aattctgcgt ttcgcagatc gcccccatct aaattcgccg aataaaatat aggaggatgt
     8821 tttatcccat ttgcaataag ctgttcgcaa acttccacga tgatcgtatt gaggatggct
     8881 gcaccgatta cagtagagga aggaccggct ttttgtacga tacctgtcag ctcacagcaa
     8941 gcatctccta tatccccatg gttatcgata actatatcag aaacctcaaa caatcgtttc
     9001 cctgatgaat gacgactagt gaccgatcgt gaatattgga cattcgtcaa actaatgatt
     9061 tttacgccct tcgcttttgc agttaacgct aaatcaataa tgattgggtt tcttcccgat
     9121 accgagtgaa ggatcaatac atcttgattt ttaaatgaaa ctgtttttgc taaattcgtt
     9181 ccatatcctt ctaaacgttc catttgactg gtaaaggtga taggggaacg atctagcatc
     9241 acttctcgtc caaagatagc attgatggtc atcatcccac ctgcccgata atacatctct
     9301 tccgctaaga taccagcatg actagcacca aaaatataga ccgaacgttt ttgcagattc
     9361 gcttgggtca atacatcgat tgcttctttg attgactttt tttcttcctt ttcaaccaaa
     9421 tctaataact gtttgatttt atccatatat ttgaacatct agaaccctcc aaacaaaccg
     9481 ctaaatcctc taccgatcat tccccataac gacaaatcgt ttccgccgaa aatcaatatc
     9541 cagctttgga tcgtcgtact aaaaacaata gctgaaaaac cagctaatac gaccatgaca
     9601 aatgaagctg cgattgttcc gacaaccgct ccacgaaagc caccttgacc ttcaccgatg
     9661 actgctgcac caccacattc aaagaaactt gtgattacta atggcacaag caacacgccg
     9721 aatagatgga atgaattcgc caaaacaact aaaatcgtag aagtgatcat tgccacgata
     9781 aaaccgataa tcactgcatt tggtcgataa ttgaataaaa taggcacatc aaatgctggt
     9841 ttcgctcccg gtacgacttt ttctgcaatc ccttgaaacg ctggaatgat ttgattgatc
     9901 agtagtcgga caccatatag cataatgagt agtcctgcac caaaattcaa ccctgcttca
     9961 atcgaaaaca tcaggagatt atccccttct ggtatcaaaa caggtgcgat gatcccaact
    10021 acggtattca tcaagaacat gactagtgca cctgtaattg agatttctcg gaaaaatgaa
    10081 agattctcag gtactttgat atcttctgtc gatttttctt tatttcctac tcgttttgct
    10141 acaaatcctg aaactaaaga caaaacaccc gttggatgac caatcaggaa agaatcatct
    10201 cccgtagcat cccagacata tttgcgcatg atccacggca taaaagacca ataacaagcc
    10261 gataaaatcc cgctgatccc aatcaatgct ccccctgaca gtcctgcttc cacaccagct
    10321 gctacaaata cgaaaggaaa ccaccaaagc atatgacctg ttaaaaagat cgtcttcaca
    10381 ggtgtaaacc gagcaatcaa taaatggata actaatccta aaaacatagc taatccgacc
    10441 gttccgccat attctgcagt aaacgtcaca tcagatagtc cttctgtcac ttctacaccc
    10501 aactgtgttt ggacagctat attgattgga gaaatcgttc cgaccagcat attgactccg
    10561 gcggtcaaag caaccatacc aaatgcagcg gtcaaagctc ccttaaccgt ttctgaaaat
    10621 gattttcgct ggatgaacag cccgagcatc gcaatcaaac ctaacaagac gggcggattc
    10681 ttcaagatgt gttcactaat gaaattcatt cttttattcc cccttatcag ctagatattg
    10741 ttccaattgc gcctttattt ctgaagtatc catatacttt ttaataggat aaacaggcgc
    10801 ttttacagaa gcttttagtt ctggaagtaa ttcatgactt gtaaaataaa catctgccat
    10861 cgttgctttg gcagtggata aatctgtatt ttccactgtc gcctttaaac ccagctcctt
    10921 aattacttta tcaagagaca ttttcaaaac catagaactc cctacaccaa atccacatac
    10981 tgctaaaatt ttcatttttc ttcctcctta tgcaaataac tgaatgattg tttctttatt
    11041 tcctgataaa atcgtttgat atttttcatt gtccataaat atttccatga tatcttttaa
    11101 tctctccaga tggctttcgt ggtttttggc agctaaaacc aaaaaaatat ggattggttt
    11161 ccctttcata tctacagcat cttttacgac taaaaggctc atactttgct cgtttactcc
    11221 aagacctggt tttgcgtgca tcagcgcaaa ataatcagcc aataccatat atggtccatt
    11281 ttcctctaca cttctgatca tgttttctaa atacgtatgc gttatcattt tgttttttaa
    11341 taatggattg ctagcgattt cgactgcttt catccaattt tcagccgttt ctattacttg
    11401 gatttgattt ttcggcagca atttattgat agtactcatt ctctgacctc caacattttt
    11461 gaaataaacc aatttttatc ctctattcct tgcatgacca ttttcatcac aggttcgact
    11521 tctttcacat cttcttctcg gatcattagc agaacaacga tatctgcctt ttgatatccc
    11581 catgaaaatg gcttgtctgc tatcaataca gcaaccgaag tttgtttaac gacattggga
    11641 ttgccatgag gaatagcaaa accattctcc aagtatgttg attggatttc ctcacgctca
    11701 aaaatagagt agagaaatcg ctgtttcgat tcttcattct caaactgttg ctgatcaacc
    11761 aaaaaacgga tagcagcttc ttttgaatca aagtgatcaa aatagacatt agaggaaata
    11821 agaggtttca ctttttcagc tatcgagcct tcgatgttta tttgttcgtt atctatgacc
    11881 agatcgatat aatgattcat gatacgtttc atatctctag cagtaaccat tggcgaaatt
    11941 cttactactg gtctaatttg tttaggtata tcgacagtgg agataatcaa atctacgtca
    12001 aacaaatcta gtctgttcaa tttatcgaca gatgctgttt cgatactatc gatatcaggt
    12061 aagattcttc tgatttttgc agaaacaaat gaactggttc ccattccatt gggacatacg
    12121 aataagagtc tatttaatcg tttgacacgc tcgattgctg cttggaaatg gatagctaca
    12181 aaacctactt catcttcgga caagcttact tgataaggcc agcgaaaatc atttaacgca
    12241 aaccagacaa caccatacaa agaaggataa ttttgtttta tctcatctat caaaggattt
    12301 acgatggtcg tttgagcctc aatacgtaaa agcagctgat acagatggac aagcagatct
    12361 tcttctaaac gactatcttg ggtaagatct atctgaatgc ctctactcac tttttggatc
    12421 aattgccgga ttttttctcg aaatactaaa gggatcgttt catttttcat gaaaaagcgt
    12481 aaaccacttc ccacaataac ataagtcaaa tactgtactt cttcttgcga aaattggtaa
    12541 atacgctgtt cctctaactg atgcagcaat tcacaaacga ggggatattt gttgaattct
    12601 aaaaaatact ttccatattg atttttacct tttaaactga ttttcttccc ttctcttgaa
    12661 cgctgaatca tcaataagat tgaaacgatg atacttgaaa agtagctttc tggtagctca
    12721 atttgcttgt ctgcaatgct ctcgcgaaaa gatcgttcga ctattgctag aagctttcga
    12781 tccactattt ctcttacgat ctccaattca tcaaaatgat tcaggatcat acgcttcagt
    12841 accttctgaa cctgtatttc atttccttca aaatatgtcc cgaaacgatc aaaactgaga
    12901 ttcaaccctt ctttcgcaaa tacatatttg atgtaagaaa tatcatttgc gatactgctt
    12961 ctggaaacat aatattcttc agaaagttgc tggtaactta catgctcgaa attggacaat
    13021 ctgttcagga tcgctactct tcgtagtttg ctttcttgat attgtattac actcatttcc
    13081 atcaccttct atattcacta taattttttc ttataaggag aactagacca aataaaagat
    13141 taaaataacc tgagaaatca tacactgctc tttctcagtt aatatacgct aaaacagaag
    13201 ctatacaaga aaattatgga actatatttc agaaaaacaa aaaaacagcc aacggacgaa
    13261 atgtccgctg actgttcaat ttgatggctc aagttttact gtctgagatg caagatactc
    13321 aaccaggcaa tgggcacata attaaagaaa aaactgattc tttggcaaaa tcctatttta
    13381 ttcaggaatg accaagaatt tatagaagga attcttgcca gaccataaat cgccgcaaaa
    13441 agcaaactga tcacagacaa tccaaggtat aagtggctta cgtccatttg cccatttttt
    13501 cgatagatca agaaaagaag aactggaaac aataaaaaac cactataacc taaacctgat
    13561 ccgatattat ggacccatgt cgagacattc cacgaagatt catgggtatt gatactgaac
    13621 aaacctgtaa agatacaatc acctatacca taaaaaccga tagatgctgt aaccagggta
    13681 gctaacacac gagatgtact tacaaatgca gcatacaatg caggcaatga caggatgaaa
    13741 aaaatccctg aaaccactga ccaaataaga aatgcttttc ttacaggact ggaaatctct
    13801 ccaaatacac taatcacact cttcatttga tctaattcag gataatacaa tcctaatacg
    13861 tacggagtca aaaaatcact caacacgcct aataataaaa agaagaaacc aaatttacgt
    13921 aaaaaatcca tgatgcgcct ccaataacaa ttacctatgc ttttaattgt tattataaac
    13981 gactttatct cgatttaaca ggatcctcta ttttacacga acatctacat aactcattaa
    14041 aaatttgaat cgtcttaagg ttcatcccta aatatctaat attcaacgtt catttaaccc
    14101 atctaatcga tctttcaatt tgattttttc tccacttctt ttacgcgcgc ctaattctcc
    14161 tattgcagtg aacaacagat cacttgagga atttaaggca gtttccattg aatcttggat
    14221 aacaccgata ataaatccga taccgacgat ttgcatcgca acatcattag agataccaaa
    14281 caaactacat gctaatggaa tcaataacag tgaaccgccc gcaacacctg aagccccaca
    14341 agcagctaaa gcagacaaaa tacataaaag gactttcaaa acaaatggtg cttcaatccc
    14401 taaggaatgt acagccgtca atgacatcac ggaaatggta atcgcagcac cgcccatatt
    14461 gatcgtagca cctaatggga tcgatatgga ataagactct ttcgtcaatt tcaatttttc
    14521 agccagcatc atgtttatcg ggatatttgc ggcagaactt cgggtaaaga aagcgggaag
    14581 cgcactttct ttcaaacaaa aaagaatcaa tggatagggg ttttctttaa taaacaaaaa
    14641 cgtgaggaac gggtacacaa ctaatgcgac aaacagcatc gtgccaatca acacaagtaa
    14701 aagctgcaga tactctgcca atccagcgat tccagtcgtg gcaatcgatt gataaaccaa
    14761 tcctaaaatc cctatcggag cgaatgcaat aatcacttgt acgacttgtg ataatgctac
    14821 agatatttga tccaccaatt cttttgtcga ttctgaacgt gctcgaaaag ccagacctaa
    14881 caacacagcc cagaataaga caccaagata attcgcatca atgagcgatt gaacaggatt
    14941 ttgtgtgaca tttgtcaaca tcgtagataa aacactttcc aaattttgcg gtgcttgttc
    15001 agatactgtt tcttgtaaag taataggaac agtaaaaatt gaacttataa tcactgctac
    15061 aagtgccgaa ag
//
LOCUS       DACCQI010000073        14987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCQI010000073 DACCQI010000000
VERSION     DACCQI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.074"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(369..995)
                     /locus_tag="IVO76_001864"
     CDS             complement(369..995)
                     /locus_tag="IVO76_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429821.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="IVO76_001865"
     CDS             complement(1290..1625)
                     /locus_tag="IVO76_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7429822.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="IVO76_001866"
     CDS             complement(1612..1896)
                     /locus_tag="IVO76_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP7429823.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="IVO76_001867"
     CDS             complement(1952..3475)
                     /locus_tag="IVO76_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7429824.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="IVO76_001868"
     CDS             complement(3468..4643)
                     /locus_tag="IVO76_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7429825.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="IVO76_001869"
     CDS             complement(4647..4832)
                     /locus_tag="IVO76_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429826.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="IVO76_001870"
     CDS             complement(4798..6492)
                     /locus_tag="IVO76_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7429827.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="IVO76_001871"
     CDS             complement(6489..6962)
                     /locus_tag="IVO76_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP7429828.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="IVO76_001872"
     CDS             complement(7031..7186)
                     /locus_tag="IVO76_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP7429829.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="IVO76_001873"
     CDS             complement(7320..7700)
                     /locus_tag="IVO76_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7429830.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="IVO76_001874"
     CDS             complement(7704..7919)
                     /locus_tag="IVO76_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429831.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="IVO76_001875"
     CDS             complement(7922..8329)
                     /locus_tag="IVO76_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP7429832.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="IVO76_001876"
     CDS             complement(8595..10070)
                     /locus_tag="IVO76_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP7429833.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="IVO76_001877"
     CDS             complement(10060..10908)
                     /locus_tag="IVO76_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP7429834.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="IVO76_001878"
     CDS             complement(10945..11307)
                     /locus_tag="IVO76_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429835.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11607..12231)
                     /locus_tag="IVO76_001879"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="IVO76_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="IVO76_001880"
     CDS             complement(12228..12539)
                     /locus_tag="IVO76_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429836.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="IVO76_001881"
     CDS             complement(12583..12876)
                     /locus_tag="IVO76_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429837.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..13717
                     /locus_tag="IVO76_001882"
     CDS             13070..13717
                     /locus_tag="IVO76_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7429838.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13778..14923
                     /locus_tag="IVO76_001883"
     CDS             13778..14923
                     /locus_tag="IVO76_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7429839.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         4323 a         3079 c         2390 g         5195 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatctcgc
    13561 agctcttcta aatacgcttt tacgatatga ctcattaatt aatgattcag aaaaaaatac
    13621 agatgagaaa aaagagtatc aagaatttta tgaaaatgat cttctaaaag agttcacagc
    13681 tcttgttact gagctaaata aacactattc aaaataataa taaactgcct cctccaaccg
    13741 cccccacggc ttgctacact atggagggaa aactattatg gcaacattca agcaatatga
    13801 aactaaaaaa ggtaaatttt ggctctatga agcctatctt ggaatgaaca aaatgactgg
    13861 caaacctgat aaggttcgta gacgaggtta caaaacaaaa aaagaagccc agcttgcctt
    13921 gtcacgcttg caagtggact acgataaaaa cggtattaaa aaagcgaaca acgaaacgtt
    13981 taaagaagtt tatgacttgt ggctagaaac atataaaacc actgtccgag aagccacctt
    14041 catgaaaact gaaatcaagt ttagaaagtg gattcttcca aaatatggaa atcttcgtgt
    14101 gaatgaagtc acggtaaaaa aagcccaaca aattgtaaat agctgggctg aaactactga
    14161 ccaatacaag gtacttcatt caacttctaa gcgtattttt agctacgcta tcaatcttgg
    14221 gattattgag actaatccac tagacaacgt attaatgcct aaaagaacca ccactaagca
    14281 taaagaaaag gtcaaagttt acagtaaaga acaactgatc accttgtttg attacctaaa
    14341 cagcaaacct gaaacttatc gaaacgactt tgataaaacg ttactacgct ttctctttta
    14401 tagcggggtt aggatcagtg aagcccttgc attgaattgg tcagacattg attttcaaga
    14461 aaatacggtc accatcaata aaacactaag ccaatccaaa aatggctata aaatcagtga
    14521 tcctaaaact gattgtagtg ctggggttct tccactagat gaaagaacaa tttctatatt
    14581 aaaaaaatgg cagattaatc agcggaaata tatgctgaca cttggtgtaa ctgatccgac
    14641 tatgattttt tgtggtatct ataaagaact aatcacacac cacgctattt atgcaagatt
    14701 gattactatt tgtgaaaaag ctatggttcc atttctcggt gtgcatgtag caagacacac
    14761 tcatgcttca atgctcttgg acgctggagc tactatggta gaagttcaaa ctagattacg
    14821 gcatacaaaa attagtcaga cgatcgattg ttacggtcac ttggcaaaag aaacgaaaga
    14881 aaagactgtt gaaaaattag tacaacactt aaatattgac tagatggcat acaatatggc
    14941 atacagtaaa aaataaaaaa aggagaaacc ctatcatatc aaggttt
//
LOCUS       DACCQI010000074        14956 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCQI010000074 DACCQI010000000
VERSION     DACCQI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.075"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(5..823)
                     /gene="purR"
                     /locus_tag="IVO76_001884"
     CDS             complement(5..823)
                     /gene="purR"
                     /locus_tag="IVO76_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7429840.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(990..1814)
                     /locus_tag="IVO76_001885"
     CDS             complement(990..1814)
                     /locus_tag="IVO76_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7429841.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(1787..2467)
                     /locus_tag="IVO76_001886"
     CDS             complement(1787..2467)
                     /locus_tag="IVO76_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7429842.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(2490..3428)
                     /locus_tag="IVO76_001887"
     CDS             complement(2490..3428)
                     /locus_tag="IVO76_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7429843.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(3673..4524)
                     /locus_tag="IVO76_001888"
     CDS             complement(3673..4524)
                     /locus_tag="IVO76_001888"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7429844.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(4678..7251)
                     /gene="mprF"
                     /locus_tag="IVO76_001889"
     CDS             complement(4678..7251)
                     /gene="mprF"
                     /locus_tag="IVO76_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7429845.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            7392..8198
                     /gene="proC"
                     /locus_tag="IVO76_001890"
     CDS             7392..8198
                     /gene="proC"
                     /locus_tag="IVO76_001890"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7429846.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(8277..9833)
                     /gene="rny"
                     /locus_tag="IVO76_001891"
     CDS             complement(8277..9833)
                     /gene="rny"
                     /locus_tag="IVO76_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7429847.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(10244..11293)
                     /gene="recA"
                     /locus_tag="IVO76_001892"
     CDS             complement(10244..11293)
                     /gene="recA"
                     /locus_tag="IVO76_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7429848.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(11402..12643)
                     /locus_tag="IVO76_001893"
     CDS             complement(11402..12643)
                     /locus_tag="IVO76_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7429849.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(12883..13461)
                     /gene="pgsA"
                     /locus_tag="IVO76_001894"
     CDS             complement(12883..13461)
                     /gene="pgsA"
                     /locus_tag="IVO76_001894"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7429850.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(13670..14554)
                     /locus_tag="IVO76_001895"
     CDS             complement(13670..14554)
                     /locus_tag="IVO76_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7429851.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         4289 a         3324 c         2602 g         4741 t
ORIGIN      
        1 cctcttattc tgaaaaataa tttcctggca caaccgtaat atttttcgtt tgagtatcta
       61 catctttgac ataaagcagt gacgtataat catcgatggc gcgatgaccg ttgaattttg
      121 attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa ttcttcaatc aagctcttca
      181 ttccgttgac tgttccgccg cctttcatga aatcatccac tacaaggaca cgtgaaccac
      241 gtttcaaact tctcttagac agttccatct tttcaattct ttcagaagat ccagacacat
      301 agttgacact tactgttgat ccttctgtta tttttgagtc ccggcgtaca ataacgaatg
      361 gcgcattcag ataataggac acagcttggg caataggtac gccttttgtg gctacagtca
      421 ttacagcatc gattttttca ccaaggtatt tagaagcaat gattcttcca acttgacgca
      481 gcagttcagg ttctcctaac aaatcagaga gataaacata tcctcctgga agcagacggt
      541 cttgttctga tagacgttct gctagagatt caatatattt tttcgcctct tcatatgaaa
      601 tttcagggat gaacaataca ccacctgcag cacccgggat tgtttccaag gttccatacc
      661 ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc actgatagaa gatttagcag
      721 attcataacg ttcggcaaag cttgttaagg aaatcaattc atgcgggtga ttcaataaat
      781 actgtgtcat gtcaactaga cgttcactgc gacgtacttt cacctattcc aactcctttt
      841 aagaaatttt atcgttttac attatataag tttttcttca aaaattcgag cattttatga
      901 aaaaataaga aaatagttcg gtatttatta aaataaaaag aagcctataa aaaatttttc
      961 gtttttataa ggcttctttt ctttataatt tagttatttt ttcttctgaa ggcaaccaat
     1021 attcgtttga tgatatttac aatcaggaac aaagcaataa agactaatgt gattgttgct
     1081 cctggaggtg tatctagata aaacgagcta gttagtcctg acagcatccc tagaaatcct
     1141 acacaaacac tgatagcaat cacgacattg aagcttttgc ctaatcgcat accgattgct
     1201 gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg caatcgggat catcacagca
     1261 atagccacac cagtaatcac attgaacatc atcgacatca aatgaacggg tagcccatcc
     1321 acgtgggcgg tatcttcatc aaaagtcaga acatacatcg gccgtttgaa taaaaagaac
     1381 aatataccga tggcaacgaa taagattcct aaaaagatga cttgctccca tgtaatggtc
     1441 acgatcgaac caaacagata ggattggata cttgttgctg aattccctcc gctcaaattc
     1501 atcaatacta acgctagcgc caaaccacca gccattaaaa tggcaatgga gatttctgaa
     1561 taggttcggt agatcgttcg cagatactcc aatatcacag ctgctaatac aacgactagc
     1621 atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta acgctacccc tgctaaagag
     1681 acatgagaaa gcgtatcagc catcagcgat tgtcttctga tcactaaaaa cacacctagc
     1741 atcggggcga ttccggcaat gaagatagcg gcaagaaacg ctcttctcat gaattcatat
     1801 gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg gatctgtctg tccgcatact
     1861 gtttgatatc ttcatgatca tgggtgatca ttagaactga tttcccatgg acatgcgcac
     1921 tatgtcggag aagacgataa aattcatttc tggattcttc atccatacct gttgttggtt
     1981 catcaagaac aaataagtca gggtcagtag caaaaatacg cgctaaactg attcgctgct
     2041 tttgtccgcc agacaattca cctattcgcc gatttcgcat atcccacatc cctacagctt
     2101 ctaaagcacg ctgtacatgt tgatgatctt tttcagagag acgcttgaac cagcggcctc
     2161 ttggatatct tcctgaacgg actaattcga tcaccgtact aggaaaacct gcattgaaag
     2221 atgcaacttg ttgaggaata taaccgatac ttaatttttt tccttcttga tttttctcag
     2281 caatctttat cattcctttt gaaggtttca gtaatcctaa cgatgctttc accaaggttg
     2341 acttggctgc tccattttct ccagttaaaa tgacaaattc acccgcatct acatgataag
     2401 agacatcttc tagtacaggt tcatcatcat agtaaaaggt caaatcagat atttcgatat
     2461 aacgcacctt acaacctcca ttctaaaact taattgatac tttcttttaa agctttcaga
     2521 tttttttcca tgacagaaat ataattttcg cctgctttga tttgttcatt agtcaagctt
     2581 tctaaaggat tcaacacttc tagcttcact cctgtttcat tcgacaatgt ctcagctacc
     2641 ttcgaagagg cattttcttc aaaataaatc actttgatgt tgtttttctt cacaaattct
     2701 tttagctcag ctaagcgact tggtgtgggt tcctgatctg gtgaaacgcc cgaaatcgct
     2761 acttgtttta agtcgtattg attcgctaaa taagcaaaag cagcatgctg agtgacaaat
     2821 gtgcggtttg ttgcatcttt tagctcatcg gagtattttt gatccagtgc ttcaagcttt
     2881 ttgatataag catcggcatt cttcgtaaaa gtttcctttt tatctggata tttttcgcct
     2941 aattcttcag caatcgttct gacttctttt atagctaata ccggatcgag ccaaacatgt
     3001 ggatcgtatt catgtgaatg accatgccct tcttctgatt cttcatgttc gtctgcttct
     3061 tgcgattcta aacggtcgat tcctttagct gcttcgatca tcaaggtctg tttgctatcc
     3121 actgaatcct ttaacgaatc cacaaaaaat tccatgtctg aactattata cacgaaaaca
     3181 tctgcatcgg aaatttctgc tctttctttt gcacttggtt cgaaatcatg cggctccgtt
     3241 ccagcaggga tcaaaagttt cacgtttcct tcatccccta cgatctcttt tgtaaaatca
     3301 tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt ttttgtctgc ttccttgttc
     3361 gtatttccac atgctgcaaa cagtaaagca cctatcatta ccgtagctgt caaaagatat
     3421 tttttcattt atatgctcct ttcaatgaac aaatcggaat tttacgattt attaacgtga
     3481 actaatgtat caaaataaaa aaacacagtc aatagaaatc catcaatttc tgatttaagc
     3541 atgtatattc ttgattaaat cgtaaaatgg atgagttgct gctcatatct agccatttca
     3601 ctacaataaa aaactgaaga ataccatttt aggattcctc agtttttcat cacctatttt
     3661 acttcttata ttttattgca gggttcgtac taaataaact tcctcacaaa aaccttttaa
     3721 cccattatat actctctgcg ctcttgaata atgttgacac aaggcaaaaa cggttggtcc
     3781 gcttccactc atcaatgcag catctgctcc gtacttgatc atacgctctt tgatttgatg
     3841 gatgactgga tgccgctttc ccgttatatc ttctaaactg tttcccattt ttttgatcat
     3901 ctgctgataa tcttgctctc ggatggcttc agcaagtccg tggatgtctt gatgttggat
     3961 ggcttgcgta tctacttctc taaacactgt acgtgtagag acactcattc ttggtttgac
     4021 caacacaacc cagcactgtg gcatagaagg taatacttgg atttcttccc ctttgccacc
     4081 gacaaatgcc gtacttccgt agacacagta aggaacatct gtaccgactt ccaaaccgat
     4141 ttctgccagt tcttctaacg aacagcccag attccataat tggttgatcc ctcttagagc
     4201 agctgcacag tctgtacttc ctccgcctaa tccagctgct actggaatat tcttcttaac
     4261 aaagatcttg acaccggttt tgatattgta tttatttttt agcaataaag cagcttgata
     4321 aacattgttc cgttcatcta ccggtaaaaa tgctttattt gtttctacaa tgattttgtc
     4381 ttctgttatt tcttcaaaaa atagatgatc tgccaagtct acgctcgaca tcaccatttc
     4441 caactcatga tacccatctt gccgtctata taatacatcc agtcctaaat ttatcttggc
     4501 tggcgctttt tcaatcaatt ccatcctctc acccattcga aaagtctaag tcgcaagtat
     4561 tctatcatag ggaaaaataa aagtatagct tatgtctgcc tagtggttga ttttgtcatc
     4621 gtttcaacag ctttcacaga attttctctt cataatccaa acaagttcta gtgattctta
     4681 taattctgtg agcacttctt ctgccaaact cggaacattt cctttttctt ttcctatcag
     4741 actgagtagc tgcatggtag caaatccgaa attacttcct ttcctataag caaaataacg
     4801 tccttcccaa tgatcgacat attttaattt cttttcataa gcagactgga aggaatagaa
     4861 aaaattacca taattatata cgatatgcat gactttttct tgaaagaaag aaaaatctgt
     4921 ttctccaaca ttagctaatg gagctaagcc tagattggct tccgagtagc ccattttctc
     4981 gtatacaata aacagattgg ctaacagata gtcagctaat tcctcagatt ctttaggtaa
     5041 gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc ggctgagcgg tgatgaagcc
     5101 aacgactttt tgatctttac ggacaatacc gacaccgcta gataatacat atgatttatc
     5161 aaatcgtccc acagagaagt atttttctcg tgcaccttga agccattttt gtgaaacttc
     5221 atttacagct tgatgtaatt cttccggcag ttgatcgtac caagtaaaat ggtatccttc
     5281 tttcatcaaa cgttgatatt caagttgctg actcagccga gacaagtctt ctgttgctag
     5341 atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa cccaattcat gaagcatcac
     5401 tacgtagctt tctgatatct tataaaacgc aagctgatag cctaaacaat ctgcttgttt
     5461 cataaactga ttcgttgctg cccgcatcat tttaggattg ccaactggat ctcctaaaac
     5521 aaagcatttg tttcctttga tctgataacc aaaaacgact tgatcttggt cgtcttcttg
     5581 gtaataaaaa taactgtaac ctgacaagcg aagctgatgg cttgtagctg tgcctccgta
     5641 tgtttccaat aaacggtcaa aacggtcttt ttcgtactca ctgccaagct tcgtagtatc
     5701 ttcagctaaa tattgataaa gcgccactaa agctagcaat gagatgccca aaccaataag
     5761 ccctgagaac cacacatcat ctgaaggaaa caaaataaaa tgatttcctg ttattcgacc
     5821 gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa acgataaaca aaaagccgaa
     5881 caagctagca tcaaagatta tagctcccca agaataaacg aattttgttc gataaaattc
     5941 ttttctggat aaccacacag ctaacagaat caagacatat accaatatca actgccaaga
     6001 taacgtacgt gtgattgtat ttaaaatacc aaaagatagt aaagcaatcg taggcagaaa
     6061 tgcctttttg acttttactg aaagtgctct agcaaggccg agtaataaaa agccaaccaa
     6121 tagattcact gtctgatcta aaaaattgaa tgagaacggc aataatactt gaaatagccg
     6181 actgattgca gacagattag tgattgttga tagaagtacc atcatgatcc ccgcaaaata
     6241 gacagcactt acgagcacga tatgcgcaac cttttggaaa aacagtcgcg gaatgttatc
     6301 taaaaatcga ttgattttga ctcctgtatg tgtgatgaac aatagaagtc ccgtcataaa
     6361 aggtagaaca taataaaaca accgataata taatagccaa actactgcta catcttgccg
     6421 aacaggcaat tgactcattc ctaagatcat caaaacgtca aaagtcccca ttccacctgg
     6481 gaccatcgtc agcattccta tcaatgtagc aatcatgaac ataggataga tcgaaagcat
     6541 cgaaatatct attctcatca acttgccgat gatcaaaaat accgcaaggg ctcccgtcca
     6601 ttgtccaaag gatgccagta gcagaccaag caagcctttt ggataaaact cgaagaacaa
     6661 tgtctttctc tttagataag caaaaaagaa aatgactggt gctaaaaaac taccgccgaa
     6721 aagccagatc caatagcttc gaaaaatact ttctgactga ataaaaaaga catcaataaa
     6781 tgtaagaaac gaccatacag acaaaccagt cagcataaat agtgcgactt ttgatacagt
     6841 ggctaaaacc atttttctat ttgtctgctt tccataaaaa ctagcccgca gacttgctcc
     6901 taccacacca ccaaaaccgg cgagattgtt gatcgtgttt gttacccacg cagcaagaag
     6961 aaactctttt ctttccattt ttggttttcc ttgtttttct aagactctga tcgtcaccca
     7021 atcatacaac aacatcggag tgacacccag cagtccagcc agaatcatga gcaggatcgt
     7081 catgcggctt tgttgtccca tcctttgcca aatatcttgc caactcatgc cattagcaat
     7141 atttgcgact tgatttgcca caaagactaa cacggagccg aaaaaaatca gttttaaaat
     7201 cagactatgt tcctttatcc attgcagcat caagtatcgt ttatttttca cattttccca
     7261 ttcctaactt gtatactttt gtgtcatacc ccaatgttat cttatttatt ttaatttttc
     7321 cactttagac ctgttctttg cttgcgatga tagaaaaaga aagatatcct aatgctaaag
     7381 gagaggataa catgaaagta ggatttatcg gcacagggaa tatggcacaa gctattattg
     7441 atggtttatt aaaaaaagga acagtctcga aagaagaggt gttcgtttcc agtggacatt
     7501 ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca tgcatgcaaa agtaatcagg
     7561 aagttgcatc acaatcagat atcgttcttt tagccgtgaa accaaaaatc attgaaaaag
     7621 tattggaaga actaagagaa tatagccaga aagtcttatt catttctatt gcttcaggaa
     7681 aatcattaac agatttagaa acagcgcttg aagatcctca ggcctccatc gtacgtgtga
     7741 tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc gatttgcaaa aatgaagctg
     7801 ccacggagga acagcttcag caagctacga tgatttttga agcaatcggc agcgtctatc
     7861 ctttagcaga atcagatttc agtacattca ttggccttgc tggcagctca cccgcattcg
     7921 tttacatgct gatcgatgcg atgggacgga ctggtgtact gaatggtttg tccaaacaga
     7981 cagccactga gattgctgca aaagcaatta tgggttcttg tcaaaaattc ctttcaagtg
     8041 ataaaaatcc atgggaactt gtagaccaag tttcttctcc tggcggtaca actgttgcag
     8101 gtgtagttgc cttagaagaa gcaggattca tcccagcaat catcaaagga atcaatacga
     8161 cgatcgagaa agatcttgag atgttgaata aaaaataatg atagcaatgt tttttcgtgc
     8221 cttacaaaaa aagaagagct gtggcttttg ccgcggctct tcttttgtat tttttattat
     8281 ttagcataat cagttgcacg agtttcccga ataacgatta ctttgatgtg tccaggataa
     8341 tccagttctt cttcgattcg tttacgaata tcacgtacta aacgaacagc gtctaaatcc
     8401 gagatttctt ctggcttgac cattacccga acttcgcgtc cagcttgaac tgcgtaactt
     8461 gattctacac cggcgaagct gttcgaaata ttttccagac ttcttaaacg acggatgtag
     8521 ttttctaatg attcacttcg cgcaccaggt cttgctgctg agagcgcatc tgctgcagct
     8581 acaaggacag agataacaga agtagcttcc acatctccat gatgagaagc gatcgcgttg
     8641 attactactg ggttttcttt gtacttggca gctaattccg caccgatctc aacgtgagat
     8701 ccttcaatct catggtcaag tgctttccca atatcatgga gcaacccggc acgttttgct
     8761 aattggatat cttcgccaag ctctgctgct aagataccag ctaattttgc aacctcgacc
     8821 gaatgcttca agacgttttg tccataacta gttctgaatc gcagacgtcc gatgatcttg
     8881 attagatctg gatgaagtgt atgagcaccg acttcaaacg ctgcctcttc cccgtattcg
     8941 cgaatacgtt catccatttc tttacgagat ttctcgacca tttcttcgat acgcgctgga
     9001 tggattcgtc catcttgtat cagtttttcc aaggtcatcc gtgcgatttc tcttcggatt
     9061 ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat cgatgattaa atcgatacca
     9121 gtcagcgtct caagcgttcg aatattacga ccttctcgtc caatgatacg acctttcatt
     9181 tcatcattcg gaagggtaac gactgaaaca gtcgtttctg aaacttgatc tgctgcgcag
     9241 cgttgaatcg ctaatgataa taagtttttc gctttgcgat ctgcttcttc tttggcacgc
     9301 tgttcagatt cttttaccat tagagttaac tcatgattca gctcttcttc agtcgatttc
     9361 atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa ttttttctag ttctttttgt
     9421 tgatcttcaa tcattttttc aacgtctttt tctcgctctt caattaattg ctgttttgaa
     9481 ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt tttctaacga gtcatcttta
     9541 cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt gagattttaa ttctaactta
     9601 ctttctctca gctcactttc aacttcagaa cggtatttct gattttcttc cttagcttct
     9661 aacaatgctt ctttttttag agtttctgct tctttttttg cactatcgat gattccagca
     9721 gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg actttgcgac cataaaccct
     9781 aaaccaagac cgacaattaa accgatgata gcgaggagaa tgttgaatac cataaaatcc
     9841 acctccatac tatctttttc atttttatca tctatacagc ttctatgata aattattcta
     9901 aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa acatgcacaa ctttattcta
     9961 atgtttgtac cccgtagtgt caactttaaa taaacaaaga aacggctttc tacttattca
    10021 ttaaacgatg gtttttaaaa taaatgacac tagtttcata taggtaaacc tttaaattga
    10081 gaaacttttc tattaaatta ttctttgtag atgaacagat atttttattt tatgtaccca
    10141 ctcgtataat taaataactc acgatttctt tattcaataa aaaaacggca ggtcaaggag
    10201 agtacctact cttcagacca gccgtttttg tttatcataa tttttattct tctactaaag
    10261 gcaattcttc ttgtcctttt tcatcttctg ggacagcgac ttcttcacca ataccataag
    10321 ctgctctaac tagagcagaa acttctgcca tcatttcagg atgattagcc atatagattt
    10381 tagcgttttc ccgtccttga ccaatccgat cttctttata agaataccaa gcaccgcttt
    10441 tatcaacaat gtctttttct actgccatgt ctagaagttc tccttcttgt gagatcccta
    10501 aaccatacat gacatctact tcagcgattt tgaaaggagg tgctactttg ttttttacga
    10561 ctttgatctt tgtacggtta ccaacaatat ctgtaccttg tttcaattgt tctgcacgac
    10621 gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga gtgatttctg
    10681 gattcccaaa catcacgcct actttttcac ggatttggtt gatgaaaata gcgattgttt
    10741 ttgttttgtt gatcgaacca gagagtttac gcaatgcttg agacatcaaa cgtgcttgta
    10801 acccgacatg tgagtcaccc atttcgccgt cgatttctgc tcgtggaact aaagcagcaa
    10861 ctgaatcgac tactacgata tctacagccc cacttgatac taaagcatca gcgatctcta
    10921 gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc acacctaatt
    10981 tttgtgcata ttgcggatct aacgcatgct cagcatcaat gaaagcggcc gttccgccat
    11041 ttttttgtac ttctgcaata gcgtgtagtg caactgttgt tttacctgaa ctttcaggtc
    11101 catatacttc gatgatacgt ccacgggggt aaccgccgac acctaaagcg acatctaacg
    11161 caagagagcc acttggaatt gtagagattt gttggtcgat tttttcgccc aatttcataa
    11221 tagagccttt accatagctc ttttcaattt ttttcagtgc agcatctaag gctgctttac
    11281 gatcatctgc caatacgata atcctcctta tatttacaaa tcagagcaac atttcattat
    11341 ccttatcata tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat attcgcattt
    11401 atcattttct taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc gcattatatc
    11461 tgatagcagc acgatctctg gcaaagtgat atttttccga aaccacgccg tctttagatg
    11521 aaatagcgat ccatactgtt cctgcagatt gaccttctaa cgtgtccggt ccagccactc
    11581 cggtgaaagc tacactgtaa tctgttcttg ctatctcacg tgcttgaatg gccatctgct
    11641 ccacacattc tttgctgact gttccatatt ctgctaatag ctccggatca atctccagaa
    11701 aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc tggaaatact tcagatacac
    11761 cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt caaactttcc gctgctgtta
    11821 cagtctgctt gttttctttc agtagttcca ctacgacttt tgctagcgaa ttatcatcgc
    11881 cgtatccata aaaatattcc cctactcgtt cttggatttt ttcttctaat gctaaaagag
    11941 cttgattccc tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc acttcatttg
    12001 gtttggcata tggtgcgatc gttggattga tctgtgtctc aatcagatct tttagctcag
    12061 tgaccagtcg cgattcacca atcccataaa agcgcaaaac tttagaaatt agtttttctt
    12121 cagaaggaaa acgttcctct agtaaaggac gtacttgttc cacaaacatt ggtttcaact
    12181 cattaggcgg accgggaaga aggatatacg catgtgtatc tgtttgataa aaagtcccta
    12241 aagcaagccc tgtacgattt ggtagaggaa ccccgccttc gataatctgg gactgttgca
    12301 gattgttttt ggtcattttt cgatgggttg tttcaaaata tgccaataat tttttgtatc
    12361 cttccgtatt ttgtatgaga gacttaccga ggtgagccgc agttgcttct tttgtcaggt
    12421 catcttctgt cggtcctaat ccgccacata agagtatgag ttcacttctt gtttcagcta
    12481 gagcaatcaa ctcttctaaa cgctgctgat tatctcctac aactgtttga taataaactt
    12541 cgatacctag atcagctagc tgttctgaca agaaagtggc attcgtattc acgacttgtc
    12601 ctagtaaaag ttctgttccg acagctatta tttctgcttt cattacttcc cgctccctta
    12661 tatagtatac gctgtatttt tatatttttt tatttaaaaa gagggcaagg agctgtgaca
    12721 aaaatcttgt cacagctcct tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc
    12781 ataagtcaca atatacataa aacatgaaaa gctgtttttt gaaattatcc cttgtgactc
    12841 agagttagac ggcaggctca caacctctcc actaaacgat acttacattg atcctttgaa
    12901 cacaccgctg tttttgatga agtaatccac acctgaatag atagtaaaga tcagacaagc
    12961 ataaagcatg atttgatcta ctggaatacc gatcaggctg aatgggatat tattgataaa
    13021 cagcaagata atagcaacca tttgtgtagc cgtcttgact tttcccggcc aagctgcagc
    13081 catgacttct ccatgttcga caagtagtaa acgcaagcct gtgacggcta attcacgaca
    13141 gacaatgatc gctactaccc aaccaggggc ctttccttgg tcaaccaaca tgataaaagc
    13201 agtcataact agcattttat ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag
    13261 gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg atactagcta aagcaaaaat
    13321 aactgcacct actagctgtg tgacagccag tgtggtgtct cctacgttaa attgtcccca
    13381 atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc atacaaattc taagtacagt
    13441 taatttattt ggtaaattca cactatcgac tcctttcttc cctattctac gtgactatca
    13501 gcgaagtttc caccgatatt atttactttt ccaaatgaat ttattcaaag gagtattttt
    13561 attgaaattg atctttttga attaacgaag aaggaccgcg acacagattt gtcacgatcc
    13621 ttctttttta tacctacaag taaacagatg cgtaaactaa tttatacgat cattctgcgt
    13681 attgaagagt aaggttcaca ttttttggac tctgattgtt ttcccctgga ttaactggta
    13741 cggattctcc attcgctttg atcgttgcat tgcttgctgc accaatcgtg atggtcgcat
    13801 ttgttgctgt ttccggtaac acggtactct gtgtttcatt tgctgccagt gtcccttgat
    13861 aaacataggc attgtctact agcacaccta accatacacg attgacagca gtaaattcaa
    13921 gggttacagg cttggtagcg tctgtgatat ttactgcgat ggcactgcca gaatcttctc
    13981 ctgtagctat tgccattttc ttttcttcac ttgattttgt tgttgattct actgtagaag
    14041 actctttggt ctgcgtgctt tgctctgagg aagaggactc ctcgactgat ccttctactt
    14101 taagcgaaga agctgtgtct ccgattattg gatcagcttg acgatcttgc caagtcatat
    14161 agcccacgac aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg
    14221 tccaaaagcg acttggatgc gattcttcct gatgcattgc ttttctagaa gtacttactg
    14281 tttcaggctg cggacgttca gggaccgatc ctgtcaattt gcttttccca tcaaaaacat
    14341 cgactaaatg gtctccatct tctcctacag cttcagcgta ttgccgaata aatgcccgca
    14401 cgtaaaattt accaggcaat tggtcaaaat catcattgtc gattgcttcc aaataacgtt
    14461 tttgaatttt tgtaatctgc tgtaattcat ctaaagatat ttttttattc aggcgtgctt
    14521 gccgtaattt ttctccgatg gtttcaaaag ccactcgttc atcttctcca gtctgtttga
    14581 ttctccgaag catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt
    14641 catgtcacag ctcctttttt ccgaataaac attgttctcc tgccaactcc tcggtcacaa
    14701 gtcacaatat ccaataaata taagaagctg ttttttgaac ttgttccttg tgactcggag
    14761 ttaaacggca ggctcacgac ctctttctta aacggtgttc aatcagctgt tcctcggtat
    14821 tagctcaaaa accggcaaaa tataaggaga tatttttccg tatgtttctc taatactcag
    14881 gtcaaaacgc tgatttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg
    14941 taataagtcc aaccaa
//
LOCUS       DACCQI010000075        14180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCQI010000075 DACCQI010000000
VERSION     DACCQI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.076"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVO76_001896"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IVO76_001896"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7429852.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IVO76_001897"
     CDS             complement(2260..2511)
                     /locus_tag="IVO76_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7429853.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IVO76_001898"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IVO76_001898"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7429854.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IVO76_001899"
     CDS             3461..3679
                     /locus_tag="IVO76_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429855.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IVO76_001900"
     CDS             complement(3968..4480)
                     /locus_tag="IVO76_001900"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7429856.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IVO76_001901"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IVO76_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7429857.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IVO76_001902"
     CDS             complement(6933..7367)
                     /locus_tag="IVO76_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7429858.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IVO76_001903"
     CDS             complement(7377..8165)
                     /locus_tag="IVO76_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7429859.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IVO76_001904"
     CDS             complement(8162..9103)
                     /locus_tag="IVO76_001904"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7429860.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IVO76_001905"
     CDS             complement(9280..9867)
                     /locus_tag="IVO76_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7429861.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IVO76_001906"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IVO76_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7429862.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="IVO76_001907"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="IVO76_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7429863.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="IVO76_001908"
     CDS             complement(12784..13719)
                     /locus_tag="IVO76_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7429864.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..>14180
                     /locus_tag="IVO76_001909"
     CDS             13905..>14180
                     /locus_tag="IVO76_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7429865.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT         3956 a         3157 c         2502 g         4565 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgtaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc
//
LOCUS       DACCQI010000076        13767 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCQI010000076 DACCQI010000000
VERSION     DACCQI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.077"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            322..819
                     /gene="dfrG"
                     /locus_tag="IVO76_001910"
     CDS             322..819
                     /gene="dfrG"
                     /locus_tag="IVO76_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7429866.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(891..2843)
                     /locus_tag="IVO76_001911"
     CDS             complement(891..2843)
                     /locus_tag="IVO76_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7429867.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2850..3146)
                     /locus_tag="IVO76_001912"
     CDS             complement(2850..3146)
                     /locus_tag="IVO76_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429868.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3346..3663)
                     /locus_tag="IVO76_001913"
     CDS             complement(3346..3663)
                     /locus_tag="IVO76_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429869.1"
                     /translation="MEKTKKLQLEDFTENEFYGTQEQQYLKAQVREELKEQGFIIDSS
                     FEGDFKTWIGVYARPKDKPTYLDPQNDKEAEEQEQYSINGFKQDFSEWFEWEIKNLKI
                     KEM"
     gene            complement(4104..4967)
                     /locus_tag="IVO76_001914"
     CDS             complement(4104..4967)
                     /locus_tag="IVO76_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7429870.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGL"
     gene            complement(4969..5241)
                     /locus_tag="IVO76_001915"
     CDS             complement(4969..5241)
                     /locus_tag="IVO76_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7429871.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(5258..5473)
                     /locus_tag="IVO76_001916"
     CDS             complement(5258..5473)
                     /locus_tag="IVO76_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7429872.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDKALEEYIRKYLPDKL"
     gene            complement(5572..6426)
                     /locus_tag="IVO76_001917"
     CDS             complement(5572..6426)
                     /locus_tag="IVO76_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7429873.1"
                     /translation="MEKEELKILEELRRILNSKNEAIVILNNYFKGGVGKSKLSTMFA
                     YLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLERI
                     IQLENEKE"
     gene            complement(6564..8708)
                     /gene="topB"
                     /locus_tag="IVO76_001918"
     CDS             complement(6564..8708)
                     /gene="topB"
                     /locus_tag="IVO76_001918"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7429874.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEIKDPIFADETFIT
                     FGFGHLVELAEPGHYDEKWQNWKLESLPIFPDRYDFEVATDKKKQFKIVAELLKQANT
                     IIVATDSDREGENIAWSIIHKANAFSKDKTYKRLWINSLEKDVIRSGFQNLQPGMNYY
                     PFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLIYQRQEAIE
                     NFKKEPFFEVEASIKVNQGSFKGVLSPTQRFKSQEELLSFVSSKQAKIGNQEGRIADV
                     QTKEKKTNSPNLFSLSSLQSKVNQLYKATASQTLKAMQGLYEAKLLSYPRTDTPFITD
                     SEFAYLKANFGKYSGFLGLDLEMVQTEPRKRYVDGSKVQEHHAIIPTKQVPTESALAK
                     MDDLQRKIYALVVKTTVAMFLPDYLYEETKIQTKVADLLFQSIGKTPKQEGWKILFKQ
                     QTKEEKEDVQTLPLVIIGERAEVDVKSVEKETQPPKAFTEGTLLTAMKTANKTVDDEE
                     AIKILQEVEGIGTEATRASIIEALKQKEYIQVIKNKLVVTEKGKLLCQAVESQHLLTS
                     AEMTAKWETYLKKIGKREGNQENFINNIKKFIVHLLEAVPTDIEKLSFSDYQEQKEKE
                     AEKSIVGKCPKCGNNIVLKKSFYGCSNYPECKFTLAEHFRKKKLTKTNVKELLEGKET
                     LVKGIKTKDKKSYNAVVKIGEKGYIDFVSFSK"
     gene            complement(8708..9325)
                     /locus_tag="IVO76_001919"
     CDS             complement(8708..9325)
                     /locus_tag="IVO76_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7429875.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLKHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKNNEKRDS"
     gene            complement(9857..11347)
                     /locus_tag="IVO76_001920"
     CDS             complement(9857..11347)
                     /locus_tag="IVO76_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7429876.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KKSMIGGRGVVLTSEEAIHENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTLIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKRFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREALGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            complement(11739..11987)
                     /locus_tag="IVO76_001921"
     CDS             complement(11739..11987)
                     /locus_tag="IVO76_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7429877.1"
                     /translation="MELAFRSRLRQMRGNKTRKQFAEEIGMKESNYLKIELGKSNPTV
                     KTLERIAKLTNSTLVIDLIPNDSEQLELQLDSEISKKE"
     gene            complement(12459..13196)
                     /gene="erm(B)"
                     /locus_tag="IVO76_001922"
     CDS             complement(12459..13196)
                     /gene="erm(B)"
                     /locus_tag="IVO76_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7429878.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(13321..13404)
                     /locus_tag="IVO76_001923"
     CDS             complement(13321..13404)
                     /locus_tag="IVO76_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP7429879.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYVDK"
     gene            complement(13425..13536)
                     /locus_tag="IVO76_001924"
                     /pseudo
     CDS             complement(13425..13536)
                     /locus_tag="IVO76_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
BASE COUNT         3950 a         2507 c         2157 g         5153 t
ORIGIN      
        1 tttttctttt tggtgtttta tctaaaagaa aaacctagct ttttttgtaa gtagttcagt
       61 tttggagtac aaaatgacaa aatgtacttc aaaatggact acttttttca ttttaactta
      121 cataccaaat tacatacaaa atgatttata aaatgcaatt gaaaatgata attgaatgaa
      181 aatttgtgat tttgtggtat tatgataata taacttatag aaaaaattgc ttattcaact
      241 aacgggcagg ataatgagaa aaggtttttt gatagaaata agaaattaga tggtaaatta
      301 gttaaaaagg ctgtgttata aatgaaagtt tctttgattg ctgcgatgga taagaataga
      361 gtgattggca aagagaatga cattccttgg aggattccca aggactggga atatgttaaa
      421 aatactacaa agggacatcc gataatatta ggtaggaaga accttgaatc aatcggaaga
      481 gccttacctg acagaagaaa tattattctg acgagagata aggggtttac ctttaatggt
      541 tgtgaaattg ttcattcaat agaagatgtt tttgagttat gtaaaaacga agaagaaatt
      601 tttattttcg gaggagaaca gatttataat ttgtttttcc cttatgttga gaaaatgtac
      661 atcacaaaaa tacatcatga attcgaagga gatacttttt ttccagaagt gaattatgag
      721 gaatggaatg aggtatttgc ccaaaaaggg ataaagaatg ataaaaatcc gtataactac
      781 tattttcatg tatatgaaag aaaaaactta ttgagttaac gggtgcgtta attgaagaag
      841 aacagtaaag ataaagcaca agagcttggg attactccaa gctctttttg tcatttgaaa
      901 taaattatat ctttccaatc aaattgttta tctgtaatcc ctaatctttg agcaggtgtc
      961 aaaacctttt tatccgctga tttaaaaggg cgacaaaagt tgtagtaagt tcttagaatt
     1021 gtaatcgcaa attgagcata cttcggattg aagttagcgt aaatatagct cttcttatca
     1081 ccacgagctg ttgttaatgg tctttcaaga gatgatatat aacgtcttat ctgttgaata
     1141 aagctattgg tcgaatggtc attaacattt aatagcatct ttgcgatttc gtttggttcc
     1201 agagcagata aatccgttct acaatcaact gaataaaatc ctttatcctt agtggctaat
     1261 ggatgtttga tagggttatc ggcatatttg cgatatcttt ctccatcttt actaatagcc
     1321 tcttcgtgga attgatgact tgttagtaat tcagttagat aatgagatgc caaagtccgt
     1381 agaaactttg ttttgaatcc tctgatatct ccccaatcga gcagttctgc ttttgcttgt
     1441 ccgaattctt ttaagcattg ttttctgctt ttcgtcttgt taacttggca aataaaatga
     1501 tgtgcatcgc tcaaccttaa ttctttcgtg aatactctat aaaaggcgtt tctaatagag
     1561 aagtcatcat cactaatcat tcgccattcg tcggcattaa ccatttgttt aattaaccaa
     1621 tagtgagcag ttgtagtata aggagcatca acatttaaac catcgacaaa tctactccta
     1681 ttagtaattt tccctaattc gtgaagataa gcattcctat tttcgctatc gttagcggat
     1741 ggttcttgtg gataataaga ccaatctaac ctatcatttt tacgactaaa agtattgaga
     1801 tggtcttctt tgaattttct tgtatcttca tttaattcat ccattgaaat attccaatca
     1861 taggcaacat cagaacggaa cacatatctt gataagacct cagcagatac aactatatac
     1921 gtttgcatat taaggtcttc aaaccccgta tatttctttg aaccttgtcc tttcttacgg
     1981 acgttattta ggtagtagtg catcttgtcc gtattaagcc acatctcgtt aaatttcttt
     2041 gtttgaaggg gttgtgtctc atatcgttct aaaaactcca aacaacgcct atacagccac
     2101 tccagcttat gatagtaggt tccaactcca attcctaaaa tatcacaagt acgactgact
     2161 gaaacccttc caacaaccat cttagcgaac attggaagaa tggtgttctt ttgttggtga
     2221 taagtagtgg tttcttctct ttttggtaga acattagtga acttcttaca cgccttacat
     2281 tgatagcgtt gagatttgcc cctacttttc ccccttttgt aaaattgctt cggctcattg
     2341 aatggggttg attcttcttc cgagcagcct tctttatgaa attgataatc tggctcaaca
     2401 tcttggatgc tattgatttc tataaggcgt ttaatttctt caacaacaga ccaattagat
     2461 aaaggggtta ctgtacagtt ttgcgataca cctctgccaa taggattgtc attgcagtac
     2521 atacctttat gacctttatc tttggaacta ccagtcattt tatatcgact tggtttccct
     2581 tttacatctt tgaatttgtg ctgttctttc ccaaaattac aacaaaacgg attgatgcag
     2641 taattatatt gaatgggata ttcagaaccg ttccatttaa aagacgttgg caagaacaac
     2701 attttgttat accttgttgc aaatcgtttt ggagttaata aatcatattg tttagcccta
     2761 tcttttaatt cttcgtttga aatcggaatt ttaacatcta caatttcatc ttccttcgtg
     2821 gctaatcttt taagcttggt cattgtctat caacccactc tatctttgat acccattttt
     2881 tcaacaatcc gcttattact tcgcttgttg gcaatccatt caataaactt cttatcgtct
     2941 aaaatgttca ataagaattc atccactgct tcgctttcag tacgctctgc atattcagcg
     3001 tattgcttaa ctatcaccct tacttgctct gatatttccc aatccacaga cttagcgttc
     3061 ttattttttg gctcaagaaa ttgcatagga aaatgcaccc ctttttgctt agtaaattgt
     3121 aaccatttta acacaccaat ttgcattcct acaataatta tttcacgttc gcatttttat
     3181 atacatttta acttacattt tgtggtgtaa attaacaaca aacgttgatt taacaataaa
     3241 aaacgagtat caaattcagt tgcaatttga tactcattta ggtttacatt ttcacttttt
     3301 gtactccaaa actgaactac ttactttttt tgctaggctt ttcttttaca tttctttaat
     3361 tttcaaattt tttatttccc actcaaacca ttcgctaaaa tcttgtttaa agccattaat
     3421 agaatattgc tcttgttctt ctgcttcttt atcgttttga gggtctaagt aggtcggttt
     3481 atcttttggt cttgcgtata caccgatcca tgttttaaaa tcgccctcaa aactgctatc
     3541 tatgataaag ccttgctctt ttagctcttc tcttacttga gcttttagat attgctgttc
     3601 ttgtgttcca taaaactcat tttctgtaaa atcttctaat tgtaattttt ttgttttttc
     3661 cattttcttt tcctcttttc ttttttttga tttttagttt tttgacaacc atttttgtta
     3721 ggcgtttgag gaagtgaagc tgttttcctc atgttttaaa agctagaaat aaattacagt
     3781 gtacgctcct ttcttttttc gttttgttgg ttcattttcg ccagtgtgtt cgtgtcgttt
     3841 gctgtgacga gaacgcaaca tagacacaaa gaaaacacaa gggcgagctt gctcgtttat
     3901 atacccttgt gttttcgact ggatatgtta aggtaacaaa cagtaaatga cacgttacag
     3961 actgcaaaat gtaccaactg aaaaaagaaa gaataccgtt gtgtgcttta tttctcgatc
     4021 agcttttaaa acatgaggaa atgtttttat aaaaaaagag tagttaccgt tttttggtaa
     4081 ctactctttt ccttttttaa ggtttaaagt cctggaagtt taggtgtttt gggtattggt
     4141 ggtgtaggtg gctgcaagct ttccattttt tgttgaattg ctttaaattc aggggtttct
     4201 tggtgttgat tttgaagcat tttttgctct attctctcca aagtcggttg aatttctttc
     4261 cctgatactt tacgatttaa ttctctttct aaggtctctt tcggactaat ggaaggcgtt
     4321 tctaagcttg aatagagttt cactccttct ctattgtaaa gccttatatc gctaaataaa
     4381 cccgttttat gaagggtttc taaattggtc ggtaagtttt tgacaacaat atcatgcgct
     4441 tgttttggtg ttgccctggc tgtcattgga tcatctgcat acatagtttc atatcgttca
     4501 attgttccta aatatgagtt gattttaggt actgccatga catacatttt tgtttcataa
     4561 cctttggctt gaagcattgt tgcggtttga ataggaacgt ctgttgttcg tcctgtacct
     4621 tcgatcacca aattataccc ttgatcgctc aaacggctta tgatcgcttc tgtcatgcga
     4681 ttagaataag gggtgacgtg ttttactacg tctttttcat aaagtttcac tagttcatca
     4741 aaattagggt gctgttgttt aaaggtatca ttatcaatga caataacatt cccttgtgtt
     4801 tcttcaagaa ttgctgatcg caaactggtt ttccctgacc ctggttgccc accaagtaaa
     4861 aaagcggttg gcgattcaac cgcttttttt ccttgaatca attcttctaa attatcattt
     4921 aagcgattct caaattgttt gtcagtaaaa ttgactatat ttgccatatt aagccacttt
     4981 ctctttattc aaaacttgtt ttgagtaacg attcattgac cgccaatact catgaacggc
     5041 ttgtaattct tctaaagaat aatcaaaaag atttacacgt tttgcaagct ttaattggtt
     5101 taataaattg acttccaata attgagagtc atttttattt aattcatggt tcaaaacata
     5161 gtttaatact cgattataaa cacttgccga taactcatta atgatttcaa tttcaaatgt
     5221 tttttcatat gtaactgcca tttcattttc actcctttta aagtttgtca ggtaaatatt
     5281 tccgaatata ttcttctaag gctttatcca taacttcttt tacgtttccg ccattctttg
     5341 ctgtttcgat ttttatgata tggtgcaagt cagcacgaac acgaaccgtc ttatctccca
     5401 ttatatcttt ttttgcactg attggtgtat catttcgttt tgctttttgt gcgcctaaat
     5461 ttcccacaat cactcacttc tttctatttc ttcttattct tattttatca tcaacaatca
     5521 caaatcacaa gtgatttgtg atttgtgatt aatcacttgt gatttgtgat tctattcttt
     5581 ttcgttttct aattgaataa ttcgctcaag catttcaaaa aatacgttct catacatgga
     5641 taaaactttt ttgtcatagc ctttgtgttc tgtaattccg tttttagacc aggtacttac
     5701 tttattactt cgcttgataa tattttggaa aaccaagtta tcttctttat gttctttgta
     5761 cagttcttcc aggtttgatt ttatcgttgc gctgtccgta tcaactaaat aaggaacaaa
     5821 accgatcata tctagtccag ggttaaattg ttcttgtaaa tcaatcaaat aggaaatata
     5881 gttttgaatg ttgtttgtac tttcttcttc tgcttgtaaa gggatcataa cgtaatcact
     5941 tgccacgatt gcattatttg tataaacgct tggcgttggt acagtatcaa taataatgag
     6001 atcatagtca ctttttaaag gtgctaaaag agtagcaagc aatctacttt cgttttcaaa
     6061 cgtccatgag cgagttaatt ttggcagtaa catcaaatca aacgtgccag gaatcaagtc
     6121 taaattatca gtcaaatgaa taatagaaga agccaagttt ccatttttca gtccttcata
     6181 aaaattgaca cgtggcaatt ctactttaaa cgtttttgct aagtcttttg tcaatgttgc
     6241 ttgtaagtcc ttatcgatca ttaaaacttt taaattaaat ttgtctgtca agtaagcaaa
     6301 catagtagat aattttgact ttccaacacc acctttaaag taattattca agataacaat
     6361 tgcttcattt ttactgttta aaatacgtct taattcttca agtattttga gttcttcctt
     6421 ctccataact acgccccatt ctttttttgt atagtaatat tgtatcatta agaatcacaa
     6481 atcacaagtg attaatcact tgtgatttgt gataagtgat ttgtgattaa tatataaaag
     6541 ccctgtttaa agggctttta tgcttatttt gaaaatgaga caaaatcaat atatcccttt
     6601 tctccgattt ttacaacggc attgtaggac tttttatctt tcgttttgat tccttttacc
     6661 agggtttctt ttccctctag taattctttt acatttgttt tggttagttt tttctttcta
     6721 aaatgttcag ctaaagtaaa cttgcattca ggataattcg aacaaccata aaacgatttt
     6781 tttaatacaa tattgttgcc acacttagga catttgccta caatactttt ttctgcttct
     6841 ttttctttct gttcctggta atcagaaaaa cttaatttct ctatatcggt aggtacagct
     6901 tccagtaaat gaacgataaa cttcttgata ttattaataa agttttcttg attgccttct
     6961 cttttaccaa tcttctttaa atacgtttcc catttagccg tcatttcagc actcgttaaa
     7021 aggtgctgac tttcaactgc ctggcacaat aattttcctt tttcagtcac aacaagttta
     7081 tttttaatga cttggatata ttctttttgt ttcaaggctt caataatgct tgctcttgtc
     7141 gcttctgttc cgatcccctc gacttcttgt aaaatcttga ttgcttcttc atcatcaacc
     7201 gttttattag cggttttcat agcagttaat aatgtaccct ctgtaaaagc ttttggcggt
     7261 tgcgtttctt tttcaacact cttaacatca acttcggcac gttcgccaat gataacgagt
     7321 ggtaacgttt gaacgtcctc tttttcttct ttggtttgtt gtttgaaaag aattttccac
     7381 ccttcttgtt ttggtgtctt tccgatcgat tgaaacagca aatcagctac tttcgtttgt
     7441 atcttggttt cttcatacaa gtagtcaggt aaaaacatgg caacggtcgt tttaacgact
     7501 aaagcataaa tttttcgttg taaatcgtcc attttcgcta atgcagattc ggtaggtact
     7561 tgttttgttg ggataatggc gtggtgttcc tgtaccttac taccgtccac ataacgcttt
     7621 ctaggctctg tttgaaccat ttcaagatca agtcctaaaa aaccgctata tttgccaaaa
     7681 ttcgctttta aatacgcaaa ttcgctgtcc gtaataaatg gcgtgtctgt tcttggataa
     7741 ctcaataatt tagcttcata taagccttgc atagctttta atgtttggct cgctgtcgct
     7801 ttataaagct gattgacttt tgactgcaaa ctacttaaag aaaacagatt aggactattc
     7861 gtttttttct ctttggtttg cacgtcagcg attctgccct cttgattgcc tattttagct
     7921 tgtttagaag aaacaaagga taaaagttcc tcttggctct taaaacgctg tgtggggctt
     7981 agaacgccct taaacgaccc ttggtttact tttatactag cttccacctc gaaaaaaggt
     8041 tcttttttaa agttttctat tgcttcctgg cgttgataaa tgagatataa agtcggtgtt
     8101 tgtacacgtc ctaatgaaaa tgtaccttgt acgccttttt gttgtaaatt caaggtatat
     8161 aaagggcttg cgttcatgcc gatcaaccaa tcggcaattt ggcgtgtttg cgcttcttga
     8221 taaaagggat agtaattcat tcctggttgc aaattttgaa aaccgctacg gatcacatct
     8281 ttttctaaac tattgatcca tagtctttta tacgttttat ctttagaaaa ggcatttgct
     8341 ttatgaatga tcgaccaggc aatgttttcg ccttctctgt cgctatctgt tgcgacaatg
     8401 attgtatttg cttgttttaa aagttcagca acaattttaa actgcttttt tttatctgtt
     8461 gccacttcaa aatcgtatcg atcaggaaaa atcggcaatg attcaagttt ccaattttgc
     8521 cacttttcgt cataatgacc tggttctgct aactcgacta aatgcccaaa accaaacgtg
     8581 ataaacgttt catctgcaaa gattgggtct ttgatctcaa aataaccgtc ttttttggtg
     8641 ctttgtttta aagcacttgc ataggctaat gcctggcttg gtttttcagc taaaataacc
     8701 gtactcatta actatccctc ttttcattgt tttttctttg atcgactgtc acgttatatc
     8761 ttgatcgata ccttctaaac gttcggcgat taattccagt ttgttcttca acttctttat
     8821 cggaacaacc attcaaaaat aaatcaaaag catgttttaa acgtggatca ttttctttga
     8881 atttaggttg tcgccctttg aattttcctt ttttcttagc aatttcaatt ccttgggctt
     8941 ggcgttcttt aattcgtttg cgttcagatt ccgcttgata cttatacaat tcaatcacta
     9001 aattattaat cagccgtctt aaattttcat cttcaatacc attcattgag ggtaaattta
     9061 agacttccag ggttgccccc ttaatttgaa tttgattcat caattctgtt aattctttat
     9121 tatttcgtcc taatcgatct aattcagtaa caacaactat atccccttca cgaatatagt
     9181 taagcatagc ttgtaattgt gggcgttcga ccgattgacc gcttaatttg tctgaaaaga
     9241 ccttagaaac gccctgtaac gcttgtaatt gccgatctaa gttctgttct ttgctactga
     9301 cacgtgcata accaattttc gccattttca accaacctct aaaattctct cggttgcaat
     9361 aaccaatcag ctatatctac tttttcaatt tcaaattgct tatcagaaat tgtcttttcg
     9421 taagcgataa aatcttgcgc atattgttgc tcattaaaaa tagccaccac ttcgtcattt
     9481 tctaaaactc gataaataaa ttttttcatt ttactcctcc tattatgctc aacttaaatg
     9541 acctattcac caagtcaatt atactgctaa aaccatatta ggacaaataa gtatactcta
     9601 ttgacctata aatgatagca acttaaaaaa tcaagtgttc gcttcgctct cactgcccct
     9661 cgacgtttta gtagcctttc cctcacttcg ttcagtccaa gccaactaaa agttttcggg
     9721 ctactctctc cttctccccc taataattaa ttaaaatctt actctctata tttctgctaa
     9781 tcattcacta aacagcaaag aaaaaacaaa cacgtatcat agatataaat gtaatggcat
     9841 agtgcgggtt ttattttcag cctgtatcat agctaaacaa atcgagttgt gtgtccgttt
     9901 tagggcgttc tgctagcttg tttaaagtct cttgaatgaa tgtatgctct aagtcaaaag
     9961 aatttgtcag cgcctttata tagctttctt tttcttcttt ttttacttta atgatcgata
    10021 gcaacaatga tttaacacta gcaagttgaa tgccaccatt tcttcctggt ttaatcttaa
    10081 agaaaatttc ctgattcgcc ttcagtacct tcagcaattt atctaatgtc cgctcaggaa
    10141 tgcctagcgc ttctctaatc tcttttttgg tcgtcactaa ataaggcttg tatacatcgc
    10201 ttttttcgct aatataagcc attaaatctt ctttccattc tgacaaatga acacgttgac
    10261 gttcgcttct ttttttcttg aatttaaacc acccttgacg gacaaataaa tctttactgg
    10321 ttaaatcact tgatacccaa gctttgcaaa gaatggtaat gtattcccta ttagcccctt
    10381 gatagttttc tgaataggca cttctaacaa ttttgattac ttctttttct tctaagggtt
    10441 gatctagtcg attattaaac tcaaacatat tatattcgca cgtttcgatt gaatagcctg
    10501 aactaaagta ggctaaagag agggtaaaca tgacgttatt acgccctatt aaaccctttt
    10561 ctcctgaaaa tttcgtttcg tgcaataaga gattaaacca gggttcatct acttgttttt
    10621 tgccttctgt accgcttaaa accgttagac ttgaacgagt aaagcgctta ttatctgttt
    10681 gtttgaaaga ccaatcttgc cattctttga aagaataacg gtaattggga tcaaaaaatt
    10741 ctacattgtc cgttcttggt atacgagcaa tcccaaaatg attgcacgtt agatcaactg
    10801 gcaaagactt tccaaaatat tctcggatat tttgcgagat tattttggct gctttgacag
    10861 atttaaattc tgattttgaa gtcacataga ctggcgtttc taaaacaaaa tatgcttgat
    10921 aacctttatc agatttgata atcaacgtag gcataaaacc taaatcaatc gctgttgtta
    10981 aaatatcgct tgctgaaatt gtttcttttg ccgtgtgaat atcaaaatca ataaagaagg
    11041 tattgatttg tcttaaatta ttttctgaat gacctttcgt gtatgaacgg ttttcatctg
    11101 catacgtacc ataacgataa acgtttggtg tccaatgcgt aaatgtatct tgattttcgt
    11161 gaatcgcttc ttcggaagtc agaacaacgc cacgtccgcc aatcatgctt ttctttgagc
    11221 gatacgcaaa aatagcccct ttgcttttac ctggcttggt agtgattgag cgaattttac
    11281 tatttttaaa tttgtacttt aacaagccgt catgaagcac agtttctaca acaaaaggga
    11341 tattcattca gctgttctcc tttcttacga aaattaatta gttagaagct acgatcaaag
    11401 ttgaatcaca acaaaaaagg caatcaacta agtttttctt aattgattgc ctggtatctt
    11461 cttaaagact tgaaattccc tcaaaaaccc gatataatgg gtttacagat atttaagtat
    11521 ctgattaata aagtaattaa atactttacc aaattttggg tctcgacttc tttaattgat
    11581 tggtggtaat caattaaggc tcgcaactta ttttcttggc ggaaaataag ttggtcgtgg
    11641 ctcttttttt gtattcttta ttcagttcgt tgtttcgtta tatctagtat accgctttta
    11701 aaaaaaataa gcaattaaaa agtgtaaaaa tattacgttt actctttttt gcttatttcg
    11761 ctatctagtt gtaattctag ctgttccgaa tcgttaggga tcaaatcaat gaccagggtt
    11821 gaatttgtaa gttttgcaat ccgttccagt gtttttacag tcgggtttga ttttcctaac
    11881 tcaattttta agtaattaga ttctttcatt ccaatttctt cagcaaactg tttacgtgtt
    11941 ttatttcctc tcatttgtct taaacgactt ctgaatgcta gctccaagtt tctcccccct
    12001 ctttttgtgt aataaaattc acgctttatt ttaaattata gcttttaata tcttaataaa
    12061 caagtgatta atcacttgtg atttgtgatt aatcacttgt gatttgtgat tattccaaaa
    12121 gtaggtaatt tcaccaacct ttttcaaaag tttttatcga cgattattgg actggatcgt
    12181 gtttcctaaa gcgattctaa gcgctcggga ccccatttag aaaaagcagg aaaatgaaat
    12241 ggtaccataa acggtcgttt atggtaccat tcaaatttat ccttattgta caaaataaca
    12301 gcgaaatttt taaatctatt ccttatcgat acaaattccc cgtaggcgct agggacctct
    12361 ttagctcctt ggaagctgtc agtagtatac ctaataattt atctacattc cctttagtaa
    12421 cgtgtaactt tccaaattta caaaagcgac tcatagaatt atttcctccc gttaaataat
    12481 agataactat taaaaataga caatacttgc tcataagtaa cggtacttaa attgtttact
    12541 ttggcgtgtt tcattgcttg atgaaactga tttttagtaa acagttgacg atattctcga
    12601 ttgacccatt ttgaaacaaa gtacgtatat agcttccaat atttatctgg aacatctgtg
    12661 gtatggcggg taagttttat taagacactg tttacttttg gtttaggatg aaagcattcc
    12721 gctggcagct taagcaattg ctgaatcgag acttgagtgt gcaagagcaa ccctagtgtt
    12781 cggtgaatat ccaaggtacg cttgtagaat ccttcttcaa caatcagata gatgtcagac
    12841 gcatggcttt caaaaaccac ttttttaata atttgtgtgc ttaaatggta aggaatattc
    12901 ccaacaattt tatacctctg tttgttaggg aattgaaact gtagaatatc ttggtgaatt
    12961 aaagtgacac gaatgttcag ttttaatttt tctgacgata agttgaatag atgactgtct
    13021 aattcaatag acgttacctg tttacttatt ttagccagtt tcgtcgttaa atgcccttta
    13081 cctgttccaa tttcgtaaac ggtatcggtt tcttttaaat tcaattgttt tattatttgg
    13141 ttgagtactt tttcactcgt taaaaagttt tgagaatatt ttatattttt gttcatgtaa
    13201 tcactcctga agtgattaca tctataaata aatacagaag ttaaacgatt tgtttgtaat
    13261 tttagttatc tgtttaaaaa gtcataagat tagtcactgg taggaattaa tctaacgtat
    13321 ttatttatct acgtaatcac tgtttttagt ctgtttcaaa acagtagatg ttttatctac
    13381 attacgcatt tggaatacca acatgacgaa tccctccttc ttaattacaa atttttagca
    13441 tctaatttaa cttcaattcc tattatacaa aattttaaga taatgcacta tcaacacact
    13501 cttaagtttg cttctaagtc ttatttccat aactttaggg ttaaccatac gcaagaccaa
    13561 tcactctcgg acaatactca tgattttaat gataaaatcc atgttaaacc catagataag
    13621 aaatacacct gcaataaccg ttacctgttt gtgccaattt aaaaatgcac cactttttta
    13681 taattaaaac ggttgaaaac tgtaccacta ataactcaca atagagagat gtcaccgtca
    13741 agttaaatgt acaaaataac agcgaaa
//
LOCUS       DACCQI010000077        13635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCQI010000077 DACCQI010000000
VERSION     DACCQI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.078"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IVO76_001925"
     CDS             complement(<1..914)
                     /locus_tag="IVO76_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7429880.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1341..1904
                     /gene="efp"
                     /locus_tag="IVO76_001926"
     CDS             1341..1904
                     /gene="efp"
                     /locus_tag="IVO76_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7429881.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2390..3649
                     /gene="lysA"
                     /locus_tag="IVO76_001927"
     CDS             2390..3649
                     /gene="lysA"
                     /locus_tag="IVO76_001927"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7429882.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3636..4436
                     /gene="dapF"
                     /locus_tag="IVO76_001928"
     CDS             3636..4436
                     /gene="dapF"
                     /locus_tag="IVO76_001928"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7429883.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4439..5320
                     /locus_tag="IVO76_001929"
     CDS             4439..5320
                     /locus_tag="IVO76_001929"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7429884.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5317..6033
                     /locus_tag="IVO76_001930"
     CDS             5317..6033
                     /locus_tag="IVO76_001930"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7429885.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6039..6734
                     /gene="dapD"
                     /locus_tag="IVO76_001931"
     CDS             6039..6734
                     /gene="dapD"
                     /locus_tag="IVO76_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7429886.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6750..7751
                     /locus_tag="IVO76_001932"
     CDS             6750..7751
                     /locus_tag="IVO76_001932"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7429887.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7748..9109
                     /locus_tag="IVO76_001933"
     CDS             7748..9109
                     /locus_tag="IVO76_001933"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7429888.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9069..10220
                     /locus_tag="IVO76_001934"
     CDS             9069..10220
                     /locus_tag="IVO76_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7429889.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEIIQSI"
     gene            10195..11364
                     /locus_tag="IVO76_001935"
     CDS             10195..11364
                     /locus_tag="IVO76_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7429890.1"
                     /translation="MMKLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11539..12918
                     /locus_tag="IVO76_001936"
     CDS             11539..12918
                     /locus_tag="IVO76_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7429891.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13199..13635
                     /locus_tag="IVO76_001937"
                     /pseudo
     CDS             13199..13635
                     /locus_tag="IVO76_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4551 a         2382 c         2963 g         3739 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaat aaaagtcaaa ggcttgaaag aaaaaaatat
     1201 ttcgatagca tcgtttaccc gtgtttattt ttctgacaaa taggcatgag accttaaaag
     1261 atagagaata ctctagtttt ctggccggtt gtctgataca ataaagttga ttatgataaa
     1321 gaaaagaagg agcaaaaata atgatctcag caagtgattt aagagccggt atgactttcg
     1381 tacaagacgg caaactgatt aaagttctgg atgcaagcca ccataaacct ggtaaaggaa
     1441 atacagttat gcgtatgaaa ctgaaagatg tccgttcagg tgcaacatac gacacaacgt
     1501 tcagaccaga agaaaaattt gaaaaagcac atatcgacac aaaacctgta caatatttgt
     1561 acacgatgga tgatacagct ttcttcatgg atttggaaac atatgagcaa tatgaaattc
     1621 ctgtagaaac agtagaagaa gaaatgaaat atatcctaga aaacatggaa gtaaaaatcc
     1681 aatttttcgg tagcgaagtc atcggcgttc aattaccaac aactgtcgta ttacgtgttg
     1741 tggaaacaca accttccatc aaaggtgcaa cagtcactgg ttcaggtaag ccagcgacaa
     1801 tggaaactgg cttagtagtc aatgttcctg acttcatcga aacagatgaa ttgttggaaa
     1861 tcaatacagc agaaggttct tatgtaaaac gtgcgtcaaa ataattcgct aacggattga
     1921 taagaatttg aaaacagagc ttgacctctt gaaaggcaga tcatcggatc tgcctttcaa
     1981 gaggtttttt gtggaatcag caaattgaaa aataaaaaag tatttgattt tatttatatt
     2041 ttgaaagatg aatggcaaaa gctagagaaa gaagaaactg cacttattta tgaagaaatt
     2101 gaaatgattc ataaaattga aaatatccga actattccga actatattga agcgaaaata
     2161 ttttttcatc actaaatagt gttgaattta tgtttttatg tataaatagc ttctaatagt
     2221 tcgggtattt tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata
     2281 tactagacaa ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca
     2341 aatgaaattc caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt
     2401 gataattggc ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt
     2461 gtatgatgaa gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa
     2521 acgttttgat acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca
     2581 tttagttcgt gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc
     2641 tttgcaagct gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga
     2701 ggaactgatc tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga
     2761 agttgaatta ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg
     2821 gttgaatgtc ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc
     2881 aaaattcggc atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga
     2941 aagcagctat ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt
     3001 tactgcttgg ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc
     3061 tctttccttg aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat
     3121 cccagtcgaa gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg
     3181 aaaactgacc attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg
     3241 tacaacacta tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt
     3301 agatggcggg atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga
     3361 tttagcagca aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg
     3421 tgaatcaggc gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct
     3481 tttagctgtt tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc
     3541 gacacgacca gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca
     3601 agcattttct gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct
     3661 gcggaaatga ttttatctta ttagattacg cagaagacac agattatagt caattagccg
     3721 cttctttatg cacaaaagaa aaatatgata cagacggatt gatcgccgta aaaatcgatc
     3781 cgttggaaat gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca
     3841 ttcgctgttt tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg
     3901 tagagacatt agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca
     3961 gcgtagatat gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc
     4021 caataaaaaa ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt
     4081 taggaacgat tcataccgtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa
     4141 gaggtgaact gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag
     4201 aagtagtcaa tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac
     4261 ttgcttgcgg tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt
     4321 cagcagatca ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg
     4381 cgatacaaat ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat
     4441 gataaaaggt tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta
     4501 tgctggtttg gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt
     4561 tttaggtaca acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca
     4621 actgacagta aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa
     4681 tacgaaagaa acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt
     4741 ggtcatcacc ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc
     4801 aattgcagaa gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat
     4861 gtcgattcca atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa
     4921 agaagcctca ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt
     4981 cttcctttac agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg
     5041 tgtaataagt gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac
     5101 gcaggacgga agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca
     5161 tgccttgttc agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt
     5221 accttccgga cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt
     5281 agctcagctt ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt
     5341 ggacagatgg gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt
     5401 ttacggacgg cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt
     5461 attgattttt ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa
     5521 cttccgttag tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa
     5581 catgccaaac atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg
     5641 aatcaactaa tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa
     5701 aaacatcata gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca
     5761 atccaagaag tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa
     5821 aaccaaattg gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg
     5881 tttgctgcta ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt
     5941 gctgcaggcg cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa
     6001 ttagaagatt tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc
     6061 gctacattca aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc
     6121 caatcgaatt taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg
     6181 actatgagac gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa
     6241 tcgaaactgc tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc
     6301 gtatcgaacc aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca
     6361 tgatgggagc gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg
     6421 gtgctgtctt aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg
     6481 tcttagcagg tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac
     6541 tggtaggtgc gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg
     6601 cagcaggagc agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg
     6661 cacgtgtatt aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt
     6721 tacgcgaatt atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg
     6781 caaccggatt agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag
     6841 gacgattgaa attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa
     6901 agaaacacac tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat
     6961 tttctgcagg tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat
     7021 ttgtcataga taattcctca gcatggcggg aagattcgga tattccatta atcgttccag
     7081 aggtcaatct ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca
     7141 ttcaagcggt acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt
     7201 attcgaccta tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta
     7261 cgcaaaatga cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg
     7321 aaatcgatca gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga
     7381 cccgtaagat tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa
     7441 tcgaaaatgg acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag
     7501 aaattagaga gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac
     7561 ataaatatcc gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca
     7621 gaaaagacac ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca
     7681 aaggagccgc tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg
     7741 tggcaagatg agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga
     7801 agagcaattc aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt
     7861 tgtttctgct ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt
     7921 gatttatgct taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga
     7981 tcgtttcatt caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc
     8041 acgcatacag aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg
     8101 tgaatatctg actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa
     8161 agacctgatt ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat
     8221 cacaaaagca tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc
     8281 tgcaggaaaa gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc
     8341 gaagatgttg caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc
     8401 tgatccgcga atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag
     8461 agagatggct tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga
     8521 ggctgatatc cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat
     8581 atcgaatcac gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt
     8641 tctttccatc actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc
     8701 aatgagcatt tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa
     8761 tatcggagtg atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga
     8821 attaaaagaa acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc
     8881 tgtcgttggg ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt
     8941 gaacaaatta gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat
     9001 catcggtgta ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt
     9061 gaatacggat gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc
     9121 atgaattcaa gacaaaaaaa tatttatatg aacaagtaaa aaacagcggg gccgttatcc
     9181 atgaaatcgg cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg
     9241 cttttcgaac agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt
     9301 ctaagcatga aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag
     9361 ggttgactga ctttataact aaacacattg aggatcttat ttataatgta gtactgatct
     9421 atcagccatc agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg
     9481 ataaatatga agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag
     9541 aagttttctc tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta
     9601 aaggccagtc tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg
     9661 tacgattttt gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc
     9721 taatgaaatt tggccaaatc caaggtggaa cgatccgaaa tgttttagct ggagaagtta
     9781 ttgtatccgg aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat
     9841 tagaccattt agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg
     9901 gctatccagc tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac
     9961 tacatgaatt agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat
    10021 atccatgtgt atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt
    10081 tcgattttga tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta
    10141 cagaaaatct tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg
    10201 aaattatcca atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa
    10261 ttcgatcagc aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat
    10321 tttccaactc ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct
    10381 cactataccg gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa
    10441 cagcgttatg gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg
    10501 gaagcgattg cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca
    10561 gcgcctgctt attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg
    10621 atagatactt ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa
    10681 caatatggat cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg
    10741 acattgtctg caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt
    10801 gttataagtg atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct
    10861 acttatcttc cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact
    10921 ggttggcgag tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc
    10981 catcaatatc tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta
    11041 ataaatgggg tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat
    11101 ctggtccagc aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac
    11161 ttgttttgca aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta
    11221 gcggaacagg caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt
    11281 tatatccgga tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc
    11341 aaagactttt taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt
    11401 gcggtatatt ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct
    11461 tttgattcat aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata
    11521 ttcaaggagg tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga
    11581 gggcatcaat cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta
    11641 tttgaagcag gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac
    11701 aaggtaacag atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat
    11761 gttcatattt taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg
    11821 gttactagcg catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt
    11881 tctggtgtca aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg
    11941 atgcgtggat acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa
    12001 aaaatcgctg tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa
    12061 gctgggaaag aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat
    12121 gctgagatga cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca
    12181 aatgcaaaaa tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa
    12241 aaagaaatcc cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg
    12301 ttaaaaggga tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa
    12361 acgaatcttc cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca
    12421 cgcaaaaaaa tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta
    12481 aaacatcttt ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca
    12541 ttaagtgtct ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa
    12601 gcaggagtag atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca
    12661 gaagaacaag gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa
    12721 ttgctaggag gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca
    12781 ctagcgatcc aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag
    12841 ccaggctttg accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa
    12901 gaaccatttg tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt
    12961 gcatcaagta ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga
    13021 cttattaccg cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca
    13081 cgatttgtgt accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat
    13141 tttttatggt taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg
    13201 gcaaaggcga ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat
    13261 tcgggagaag cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg
    13321 tttctctatc aataaaaaga aagttcagcg cttgatcaag aagctaggaa taaaagtaac
    13381 ggcatacacg cgtaaatcac gtcgctacaa ttcttatcga ggaaaagttg gagcagtagc
    13441 caaaaatcgg atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga
    13501 tacaactgaa tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt
    13561 gtatctagat ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga
    13621 aaagcccaat gcatt
//
LOCUS       DACCQI010000078        13375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCQI010000078 DACCQI010000000
VERSION     DACCQI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.079"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(264..2390)
                     /gene="pnp"
                     /locus_tag="IVO76_001938"
     CDS             complement(264..2390)
                     /gene="pnp"
                     /locus_tag="IVO76_001938"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7429892.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2578..2847)
                     /gene="rpsO"
                     /locus_tag="IVO76_001939"
     CDS             complement(2578..2847)
                     /gene="rpsO"
                     /locus_tag="IVO76_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7429893.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            3098..3661
                     /locus_tag="IVO76_001940"
     CDS             3098..3661
                     /locus_tag="IVO76_001940"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7429894.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3914..4435)
                     /locus_tag="IVO76_001941"
     CDS             complement(3914..4435)
                     /locus_tag="IVO76_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7429895.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4438..5280)
                     /locus_tag="IVO76_001942"
     CDS             complement(4438..5280)
                     /locus_tag="IVO76_001942"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7429896.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5273..5998)
                     /locus_tag="IVO76_001943"
     CDS             complement(5273..5998)
                     /locus_tag="IVO76_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7429897.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            6174..6470
                     /locus_tag="IVO76_001944"
     CDS             6174..6470
                     /locus_tag="IVO76_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429898.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            7038..8630
                     /locus_tag="IVO76_001945"
     CDS             7038..8630
                     /locus_tag="IVO76_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7429899.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8643..9383
                     /locus_tag="IVO76_001946"
     CDS             8643..9383
                     /locus_tag="IVO76_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7429900.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            9551..10900
                     /locus_tag="IVO76_001947"
     CDS             9551..10900
                     /locus_tag="IVO76_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7429901.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(11028..11618)
                     /locus_tag="IVO76_001948"
     CDS             complement(11028..11618)
                     /locus_tag="IVO76_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7429902.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            11765..13117
                     /gene="brnQ"
                     /locus_tag="IVO76_001949"
     CDS             11765..13117
                     /gene="brnQ"
                     /locus_tag="IVO76_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7429903.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         3750 a         2648 c         2642 g         4335 t
ORIGIN      
        1 cgagggacag ctgattgaac atcgtttatt ggagaggttg taaaaaagct gttctgcatc
       61 aagtaataag aacagccaag taaaaacagc tcctaatgtt tttaacttgg ctggacttat
      121 taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaggactg tgacacggct
      181 tttgtcacag tccccttttt tcgaataatt attgtccccc tgtcaccttc acgatcacaa
      241 gtacattatt cctatgaatt aaattatttt tcttcatttt tcgttgaacc atcttcgttc
      301 aacattgctt ttcttgacag atttacgcgt ccttgtttat cgatttcagt tactttcaca
      361 aggatctcat cacctagttt aacaacatct tcgactttat tgacacgatc gtttgctaat
      421 tgggaaatat ggacaagtcc atcttttcct ttgatcaagt tgacgaatgc accgaatttt
      481 tcgatacgga caactttccc tagataaact tcaccaactt tgacttcttt ggtcaaatct
      541 tcgatgatct tgatagcttt tttgatcatt tctgcatcag ctgaagcaat acttactttt
      601 ccatcttgat cgatatcgat tttcacacca gtttcatcga tgattccgtt gatcgtatcg
      661 ccgcctttac cgataacatc tttgatttta gcaggttcga tctggatcat ttcgattttt
      721 ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg ttgaagtcaa ttcttctaag
      781 atttccatac gtgcttgttt tgcttgagtt aatgcttcag ttaggatttg ttctgtgatt
      841 ccttgaatct tgatatccat ctgtaaagca gtgataccat cttttgtacc ggcaacttta
      901 aagtccatat cgccaagatg atcttccagc ccttggatat cagttaggat cgtatagttt
      961 tcaccatcag aaacaagtcc catcgcaata ccggcgactg gcgctttgat tggtacacct
     1021 gcatccatca atgctaatgt gccagcacag atacttgctt gggaagaaga accatttgat
     1081 tccaatactt ctgctactaa acggatcgta taaggaaatt cttcttctga agggattact
     1141 tgtgctaacg cacgttcacc taatgcgcca tgtccgattt cccgacgccc tggagagcct
     1201 gcacgtcctg tagaaccaac agaaaattga gggaaattgt agtggtggat gaatcgtttg
     1261 ctgacttcca cccctaaacc atcaatgatt tggtgctctc ctagaggtgc gagtgtacaa
     1321 gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg atccatgtac acgtggcaat
     1381 aagctgacct ctgatgacaa tggacggatt tcatccaatt tacgcccgtc aggacggatc
     1441 ttatcgatgg tgatcaattc acgtacgaca tctttttcaa gatcttctgt gatttgttta
     1501 acttctttca acaagtgatc ttcatcttca tgacctgcaa atttctcagc ataagcttcg
     1561 cggatatcga tcttcacctg ctcgatatta tcttcgcgag caagtttttc ttcagtcatt
     1621 actgctgctt tcattgtagc gtaagaagca tcaaagattt cttttttcaa tacttcatca
     1681 acttgcagta aagtcacttc cattttttct ttccctactg cttgaacgat ttcttcttgg
     1741 aaagcaacta actctttgat agcgtcaaaa ccaaacagca atgcaccaag catgtcttct
     1801 tctgaaactt ctttcgcgcc gctttcaacc atattgatgg cttgtttcgt tccggcaaca
     1861 gtcaattcga tatctgtttg ttctgcttgt tctactgttg gattcaagac gtactcgcca
     1921 tttacacgac caacttctac gcctgcgatt gggccgtcaa atgggatatc tgagattgat
     1981 agtgctaaag aagaacctag cattgctgcc atagctggcg agcagtctgt ttctacactc
     2041 atcacgatgt tcgttacttg gacttcatta cggaagcctt ctgcaaacat cgggcgaatt
     2101 ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac ttggacggcc ttctcgtttg
     2161 atgaaacctc cagggatttt ccctgctgca tacatttttt cttcataatt gatcgttaat
     2221 gggaaaaagt ctgtatcttt tgcttcttta gaggccacag ctgcacttaa gactactgta
     2281 tctccataac ggactaaaac ggctccgttt gcttgttttg ctagctgacc gacttcgatt
     2341 tgtaagggac ggccgcccca tgtcgttttg aatacttgtt tttctgacat aacttctcct
     2401 tttccttctc gtgaaagcaa gacgctacta aacaaatgaa aactgccgaa agccgtcttt
     2461 tgagcagctt tcatttggtt agtaggagtc catctagctt gatcacggaa ttttacttaa
     2521 ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc actctttttg actaaaatta
     2581 acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt tgaacgtcag ttttacgtaa
     2641 gtaagctaac aagttacggc ggtgaccaac ttttttcatt aggccgcggt atgaatgatg
     2701 gtcttttttg tgcacgcgtg catgttcatt caagtggttg atttcttcag ttaatacagc
     2761 gatttgtact tctggagaac cagtatcccc ttcatggcga gcgtattcgt tgatgatttc
     2821 attcttgcgt tcttttgaaa ttgccatttc tttcacctct ttcgattatt cccctgcgac
     2881 tgagtaaaac gttggtgatt cgcttaacca agtggcaggt catgtgccta gagcacacag
     2941 ctatacttta cctgaaaccg ggttgaaaat caagtagtta atcattaaga aaaggtagtt
     3001 ttcaaacaat cttactacta cttttccatt tttgtggtaa actataatgc gaaaatagac
     3061 ttgaaacaca gtcttttaag aaaggggtac atattccatg attacaatgg atgatatcat
     3121 tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa gtttctcttc cattaagcga
     3181 agaagatatc tctttaggaa aagagatgct tgagttctta aaaaacagcc aagatccaat
     3241 caaagctgaa gaactacact tacgaggcgg tgttggctta gctgctcctc aattagatat
     3301 ttctaaaaga atcattgctg ttcacgtacc aagtcctgac ccagaggcag atggaccttc
     3361 tctaagtact gttatgtata atcctaaaat tttgagccat tctgttcaag atgcttgttt
     3421 aggggaaggt gaaggctgtt tatctgttga tcgagaagta ccaggatatg ttgttcgcca
     3481 tgccaaaatc actgtgtctt attatgatat gaatggtgaa aaacacaaga ttcgtttgaa
     3541 gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat atcaacggtg tgatgttcta
     3601 tgaccacatc aacgatcaaa atccatttgc actaaaagaa ggcgtattgg tgatcgagta
     3661 gagaattggc aggttccttc gatactatgc tacaagaaag ctttataaaa aaggggagtg
     3721 ctcaaaagct gcacttccct tttttatgtt tctccactaa tcgaaattta gatagacatc
     3781 tagaaatcgc aattccaaaa aattctgtgt attggatacg catcttttct tattttttct
     3841 tatttcttcc atatgggtgt ttatctcaga gatgatagaa tagtttctag gataaaaagt
     3901 agcagctacg atgttatcct tcgtagagga ttcctgcttc agctagtttt tgtttgatgt
     3961 ttttcagcat atgcttttct gggtagcgga tcgtatgcag atggatgccg cctgttaatt
     4021 cggataacaa agtagcatta cttttttgat aacgtctcat aaaggaatcg atatcttgtg
     4081 gcgttttgat gtgcaaagtt cctgctaact caccgtaaag gggatgttcc acaattacat
     4141 caatgatttc accaccattt tcaacgatta gtctcagttc ttcttctgtt tgatcttttc
     4201 catgctggac agcgatttta ctgatgtgcc cagtattggt ttcttctgtt tcgaggatat
     4261 aacctcttgc tgttgccacg atacttttac ctgcagctcg tagtaaagca atatcgccta
     4321 caatgatctg acggctgact ccaaaagttt tcgcaaactt gctagcactc actggttttt
     4381 ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt ctctccttcg atcacactta
     4441 ttcctccttc attttgtttt ggtattcgcg cgagtgtttc atcaaatcaa catacgtgtc
     4501 tgttattgtt tccttatatc ccttattttt cagcagacta acaactttat ccaatacttg
     4561 ctgctctctt tttgcatcat aaactgcttg gttttcttct tttttcactt gtcctatttt
     4621 agttaccgta tccattcttt tttctaataa agcaaccaac tctcgatcga tcatatcgat
     4681 cttatgacgt aatgtttcaa gagactcttc tgttagttct tcctctactg tcttgatgat
     4741 acgagcagga tttcctgcaa tcactacatt gtcgggaaaa gatttggtca caaccgaacc
     4801 tgcgccgact actacgttgt cccctagagt gacacccggc gtgacgataa ctcctccgcc
     4861 taaccaaaca ttattaccga ttttaatggg ttttgcgtac tccagaccgc tgttacgttt
     4921 cactggatgt agtggatgcg tcgccgtgta aagctggaca ttcggagcga acatgcagtt
     4981 atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa ttgaaatttg cataaaaatt
     5041 ctccccaaca aatatattat atccgtaatc aaaattgata actggctcca tatagatttt
     5101 tttccctgta cggccaaaag tctctttcaa caattgggat cgcagttccg ctgattctgc
     5161 ttggttgatg atttggcttt gacttcttgc aaacatacgg tcggcaacca gctcaggatc
     5221 attggcaaaa taaagctcgc ctgcaatcat tttttctttt tcggttttga attcagtcat
     5281 aatctgctcc taattcttgt atttttttga aaacaaccgc tccgtcttct tgaataaacg
     5341 tataaggctc cgctaaataa acaggcgttt gattgattac taagattttg gctgacggct
     5401 ggcgaaactg taccagatca caaaacggat gaacttgaaa actcgttcca acaatgacga
     5461 tcagctctgc ttggctgacg gcataagccg cctgctccat gactccatct tgaaaccctt
     5521 caccatataa cacgatgtct gggcgtattt gtcctccgca tgcttgatgt ttgtcgctgt
     5581 gaagatattc tttccagtct accgtttgtt gacattttcg acaatgacat tgatacagac
     5641 tcccatgaaa attgacgagt tgcgggcttt tagcctttgc gtgcaaaccg tcgatgtttt
     5701 gggaaacaac ccaaacattt ttttctttcg ctagatgtgc tatttccaaa tgaatgatat
     5761 tgggttttgc ttcaggatga taaatctttt tgacaaattg atagaattta tccggttcat
     5821 tcaccattgc ttgatgactc agcaagtatt ccggctgctc catcccgtgg tagattcctg
     5881 acaaagatcg atagtctggc actccagaag gtgtggatac ccctgcaccg gtcaaaaatg
     5941 taatttgctg ataagctaat atctcttttg ctgcttcgtc tactgttaat tgtttcatgt
     6001 tttgtcacct cactttccat tatagcccat actcgaacaa atttattgtt gaaaagtcag
     6061 aaaaatggga ataaaaaagt gttatgggca tttgagcaat ttatcacaat gttcactgac
     6121 aaaagaatga aaacgtgtta caatactgct gtataaaagt gaggtgaatc gatatgttac
     6181 cattctttat ttattggggc gttattctgg cttgtatcgc ctggttagcg ttaagcgttt
     6241 atttctctgt tttttatctt gtacgcaaag aaaatggaaa tctttgggct tttgctttat
     6301 tcaacgtgat tgccgcaatt gttttagcca ttacattagc cgtatatcga acatggggct
     6361 ggggaatcac ccaatactca agtttgattt atctaatatt agccatttat ggcgtcgttg
     6421 ttcttttgca agcgatccta ggacgtgagc caaagaaagc ggcagcttaa atgatcgaga
     6481 tggaactgga cacaggaaat tttctgttct agttcttttt ttattgtatt acatacattt
     6541 ttgtgtataa ttatactatc gtttgcaatg gatagatgat tcacattttg attgtttttt
     6601 cggtaaaagt aaagatttct atatttatca gtattttttc aaaaaccagc agttatagat
     6661 tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa ataactgaat acgagatgcc
     6721 tcaatcaatg aggtagaggt cgcgagaatc attaaccctg tggagtgaag caaacatgga
     6781 gaagcaggaa tagggaggat cgccgaaatg tgcacttttt tgcttggagt gtatgttggg
     6841 acgcaagtta agagcttgcg gactgtctta gcagtgatgc taagttgcgc tatgttttgt
     6901 gagaggttta tcaccttatg gaattttagc ggccttttta tatctataaa tcggtcgctt
     6961 tttatttttt cagataaagc tttaccgcat ttcatctaga ccgctcgtgt atcatataat
     7021 cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt tgccgtacta tttgcttttg
     7081 tcttctcact attcagtgtg ggatctctgg caaatgcaga tgaaaaaaca ccgagcgatg
     7141 agtttcgcgt aggtatggag gctgggtatg cacccttcaa ctggtctcaa caaacagatg
     7201 agaataatgc cttgccaatt caaggccaaa atagttacgc cggcggttat gatgttcaaa
     7261 tcgctaaaaa agtagcagat ggtttaggga aaaaattagt catcgtccaa acgaaatggg
     7321 atggtctggc tcctgccctt caatcaggca agatcgacgc gatcatcgcc ggaatgagcc
     7381 ctactgctga acgacgaaaa gaaattgatt ttaccgatcc ttattacgaa tcacaattag
     7441 tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa aagtttagca gatctttctg
     7501 gagcaaagat cactgctcag ttaaatacat tccactatac tgtgatcgat cagataccag
     7561 atgtgcagaa acaacaagca atggataatt tctcagcaat gagaacagct ttagcttctg
     7621 gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt aacggcaact agtgtgaaca
     7681 aagacttgga aatgcttgaa ttcagcggtg cagatggctt ccagacaaac ccggaagatg
     7741 ttcagatttc tgttggtatg cgtaaaggcg acccggatat ccaaaaagta aaccagattc
     7801 tttctggcat ctctaacgat gaacgaacca aaattatgga ccaagcagtc aaagatcagc
     7861 ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc tgatttcact acgatttgga
     7921 aacaatacgg caatatgttc ttacgaggtg ctggacttac cttgttcatc gcattgatcg
     7981 ggacagttgt cggtactaca cttggattac tgattggtgt cttccgttcg gtacctgaat
     8041 caggaaatgc actgactcgc ttcttccaaa aaatcggaaa tgcacttctt tctatctatg
     8101 tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat ggtcatcttc tacggtctgg
     8161 cactcgcttt tggcatttca ttgaaccgaa cgattgccgc tttgtttatt gtctctctca
     8221 atactggagc ttacatgaca gagatcgttc gtggggggat ttttgcagta gatgaagggc
     8281 agttcgaagc tgcgcaagcc attggtatga cccatggcca aacgatgaga aaagttgtta
     8341 ttccacaagt tttacggaat atcttgccag caacaggaaa tgagtttgtt atcaatatca
     8401 aagatacagc agttttaagt gtaatcggcg tagctgattt gttcttccaa ggaaactctg
     8461 cttccggtgc gaatttccaa ttcttccaaa catttacgat cgtcgggatc atctacttag
     8521 tcatgacctt tacgatcacc agaatcctgc gggcggtaga aaagaaaatc gatggtcctt
     8581 cagcttatac caagcttgaa gaaatcgaca attcaaattt gcaggaataa ggaggaagaa
     8641 tcatgaatga aaaaattatt gaaatcaaac atctaagaaa aagcttcggt gaaaatgaag
     8701 tattgaaaga tatcagcgta acagtcaata aaggtgaagt tgtgacgatc atcggttctt
     8761 ccggttctgg taaatctact ttcttgcgct gtatcaattt actggaaaaa ccaacaggcg
     8821 gcgagatcat ttataatgga gaaaatgtat tagctcctaa atataattta cctaaatatc
     8881 gaacgaatct aggtatggtt ttccaatcct tcaacctgtt caacaatatg aatgtcttgg
     8941 aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga taaagaaact gccaaaaaag
     9001 tagcaatcga aaatctggaa aaagttggta tggatcgtta tatcgaagcc aaaccagcac
     9061 aattatctgg tggacagaaa cagcgtgtcg caattgctcg ggcattatca atgaatccag
     9121 atgtattgct attcgacgaa cctacttcgg cgttagaccc tgaaatggtt ggcgaagtgc
     9181 ttaatacgat caaagactta gcacatactg gcttgacaat gcttatcgtg acgcatgaga
     9241 tggaatttgc aaaagatgta tctgaccgtg tcatttttat ggataaaggt gtgattgcag
     9301 aagaaggtac accagaagat atctttgtcc atccgaaaga agaacgtaca aaagaattcc
     9361 tttcacgtat tttaaatgct taggatcaat agctgattgg gacaaagagc tgcggtgttt
     9421 accgcagttc ttttttatga attactcata aaaggtaaat ttctgaaaat tttggaatct
     9481 tagttgtgtt tttcagaaaa ctctcttaat atggaagtaa ttaaactttt ttagttagga
     9541 tgatttaaaa atgaatgaag agcaagaact gcagcgtgga ctgaaaaata gacatgtcca
     9601 actgatttct attggcggag cgattggaac ggggttgttt ttaggttccg gaaagtctat
     9661 ccagctagct ggcccttcga tattgctcgc ctatcttatt actggatgca tctgtttttt
     9721 cattatgcgt gcgttaggcg aacttctgtt atcaaacacc aataaccatt cttttctgga
     9781 ttttgttgca gaatatcttg gcaaaaaagc ggctttcatc actggctgga cgtattggtt
     9841 ctgctgggta tcgattgcaa tggccgattt aacagccatc ggaatgtatg tacgctactg
     9901 ggcgcctggt gttcctcagt ggcttcctga actgattgca ttaggtcttc ttctttgctt
     9961 gaatatggtg gctgtctcat tgtttggtga gttagagttt tggtttgcat tgatcaaagt
    10021 tgtagcgatc attgccttta tcctcgtagg agcttatatg atcctcaccc actatacgac
    10081 agatattggc acagccagta tcacgaatct atggtctcat ggcggattct ttccaactgg
    10141 tgcaaaagga tttatcttag ctttccaaat ggtaaccttt gcttttgctg ggatagaatt
    10201 agttggatta gtcgctggag aaacagaaaa tcctgaaaaa gtgttgccgg aagctatcaa
    10261 taatattcct atcaggatca tcttatttta cttaggatct ctttttgtca ttatggcaat
    10321 ctatccatgg aacagcttga atccagataa cagtccattt gtagaagtat tttcggaaat
    10381 tgggatcacg attgctgctt ccttgatcaa tttagtcgta ttaagtgcag cagcttctgc
    10441 ctgcaacagt gcaatatata gtacaggacg tatgcttcgt tctttagcgc aagaaggcag
    10501 tgcccctaaa aaattcaaaa agttaacaac acatcatgtg ccagggaatg ctttgacatt
    10561 ttctactatc gtcattttca tttctgttat cttgaattac gtgatgccta gtgaagtatt
    10621 cacactagta agcagtattg ccactacttg tttcttgttt atctggagta tgcttgtcta
    10681 cacgcacatg aaatatcgta aaagtatcct cggcaaaaaa gctcattcat ttaaaatgcc
    10741 gttgtaccca tttagtaatt atctggtatt tctttacatg gcctttgtgt gtgtcgtctt
    10801 gttcctagga aaagatacga gaatcgcttt attactaaca cctgtgtggt tcttgcttct
    10861 attactaatc tatcatatga aatatgaacg taagaagtaa gaaagtagtt gtgacagaag
    10921 tggtcagctt cgagaaataa gaaggaattt acgaaaattg cttcttaaat ttttgtgaaa
    10981 tttcggctta tttcttggag ataaaagctt ctgtcccagc ctcttttcta tttccccgga
    11041 aacaaccgct gataataggc atctgctttt ttataggaaa aaatccaaat accagataca
    11101 aaaaaacgtc cgaataaccc gatcaacggt acaaaaaaga caccggcaat caagcataag
    11161 ttaatcatca tggagataag acttcgacgg tcctttgaca aaactctcac gacatgggaa
    11221 tctggtttat ttgctttcat gatttgcctc atcaagatac caaaagaaaa actccacatc
    11281 gtgtatacta agacataagc catctcaggc caaaaagctt cagggttacg cccgacccaa
    11341 ctagtaacag aaggacataa agtcagccaa aagagcaaaa agatattcga ccagaccact
    11401 cggtaattaa ttcgttctgc catctgaaat atacgatgat ggttcaccca gatcaccgca
    11461 acaaatataa agctgatggt ataagctaat aatgtatttc ggatgaccca taaagaatga
    11521 aagctagatg tattagggat cttgatctct aatacaataa ctctcaaaac aatggctacc
    11581 actccatcag taaatgcttc taatctcgac tttgtcacga tgattcctcc tcagatgatt
    11641 cagcgctttt attttatcac ttacgttatt aaataaccat cgttttatat agggaatttt
    11701 ctgaaaattg ttgcatattt ttcgaaattt tgttactttt aaaaaaaccg taaaggagtt
    11761 atgtatgaca aaaaaattat catttaaaga ctatcttttt ataggctcca tgcttttcgg
    11821 tctatttttt ggtgctggaa atcttatctt tcccgttcat ttgggtcaag aagcaggcgc
    11881 tgctaccttt tgggcgaatc taggtttctt ggttaccggt atcggtcttc ccttcttagg
    11941 ggtgattgcc attggtgtat cgaaaagctc aggtgtatat gaattagcaa aacggataaa
    12001 tcaaacatat gcgatggttt ttaccatctt gctttactta gttatcggac cgttttttgc
    12061 tttacctcgc cttgcaacca catctttcga gattggttta gcaccattta tcgatcctag
    12121 cagacagacg ctatatctag caattttcag tattgtattt tttgtgctgg cttggtggtt
    12181 ttcaagaaaa ccgacaaaga tattagatta tgttggaaag ttcctgaatc ctgcattttt
    12241 ggttttatta ggtgctttgc ttctccttgc ttttttgaat ccactaggtt ctgtaaatca
    12301 agcgcctatt cagccaaatt atcaagagca tgcttttttc acagggttta ctcaaggata
    12361 taacacatta gatgcgcttg ctgctttagc atttgggatc atcattgtca caacaatcca
    12421 aaacatgggt gtaacaaaac ctgctgaaat cgctaaagac acgatcaaat ctggtgcgat
    12481 cagcattata ttgatgggaa tcatttatac tttgctcgct tatttaggtg ctatgagctt
    12541 aggtagcttt gctctaagtg aaaatggcgg gatcacgctg gctcagattg ctgatcatta
    12601 tttaggtact tatggcagta tcttattagc atttatcgtt atattagctt gcttgaagac
    12661 cggtattggt ttgattacag cttttgcaga aacattcaca gatttgtttc cgcaaagaaa
    12721 ttacgcattc tttgttgcac ttgctagtat ccttccttgt ttagcggcaa acgtaggctt
    12781 gacgaatatc attcaatttt cgttgcctgt tttgatgttc atctatccat tagcaatgac
    12841 actgatttta ctagtgttag ttggtccttt gtttaagcag cgtacggctg tttatcgaat
    12901 gactacttat tttacattga ttgcttcgat ttttgatggg ttgaacgctt gcccagaaac
    12961 tatcaaacaa acacctattg ttcaaaatct tttatatgtt gcggaaagct acttgccgtt
    13021 tttcaaattg gggatggggt ggatcgtacc agcagttatc ggttttgtta tcggactgat
    13081 ttggagtttc gctaagaaag aagaagtagc agattgataa aagaggttgt gaaagagacg
    13141 ttcagccttg agcaataaaa agtaattttg aaaaatggct tctcacattt ttgcataaat
    13201 ttgacttaat accgagaaac tgacttttga acaccgttta ttgagaggtc gtgaaaaagc
    13261 cgcttagttt caagtattaa gaaatatttt gtgaaaatgg cttctcacat ttttgcataa
    13321 atttgactgt aagcgcccca tagaacagta cctgtacgtc aaataacccg accta
//
LOCUS       DACCQI010000079        13264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCQI010000079 DACCQI010000000
VERSION     DACCQI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.080"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            12..350
                     /locus_tag="IVO76_001950"
     CDS             12..350
                     /locus_tag="IVO76_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429904.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(555..1130)
                     /locus_tag="IVO76_001951"
     CDS             complement(555..1130)
                     /locus_tag="IVO76_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7429905.1"
                     /translation="MCGRYFFDLESELLQNYYREAQKKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1495..2076)
                     /locus_tag="IVO76_001952"
     CDS             complement(1495..2076)
                     /locus_tag="IVO76_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7429906.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDAEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2259..2885)
                     /locus_tag="IVO76_001953"
     CDS             complement(2259..2885)
                     /locus_tag="IVO76_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7429907.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(2907..4235)
                     /locus_tag="IVO76_001954"
     CDS             complement(2907..4235)
                     /locus_tag="IVO76_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7429908.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEMTNNGEKVIVEGERFFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4393..4794)
                     /locus_tag="IVO76_001955"
     CDS             complement(4393..4794)
                     /locus_tag="IVO76_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7429909.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAEMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(4880..5086)
                     /locus_tag="IVO76_001956"
     CDS             complement(4880..5086)
                     /locus_tag="IVO76_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429910.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5181..6683)
                     /locus_tag="IVO76_001957"
     CDS             complement(5181..6683)
                     /locus_tag="IVO76_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7429911.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTNPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTRYAQDLVTKIKKGMGTQDDKPLKGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(6701..7156)
                     /gene="argR"
                     /locus_tag="IVO76_001958"
     CDS             complement(6701..7156)
                     /gene="argR"
                     /locus_tag="IVO76_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7429912.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7254..7871)
                     /locus_tag="IVO76_001959"
     CDS             complement(7254..7871)
                     /locus_tag="IVO76_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429913.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYDEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8092..9465
                     /locus_tag="IVO76_001960"
     CDS             8092..9465
                     /locus_tag="IVO76_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7429914.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEKLQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            9540..10652
                     /locus_tag="IVO76_001961"
     CDS             9540..10652
                     /locus_tag="IVO76_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7429915.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTSHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(10724..12361)
                     /locus_tag="IVO76_001962"
     CDS             complement(10724..12361)
                     /locus_tag="IVO76_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7429916.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILKTYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(12529..13137)
                     /locus_tag="IVO76_001963"
     CDS             complement(12529..13137)
                     /locus_tag="IVO76_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7429917.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPVAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DATYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3549 a         2775 c         2391 g         4549 t
ORIGIN      
        1 gccatcctat catgcctgat tataaacaaa aagctggcta ttccttttgc tctgacaaat
       61 ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt cggattaaag
      121 aatgcccgat tcaaatagaa acattggttc aacgaatttc aactagacaa gactttgcta
      181 ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat gatccaactc
      241 atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat acaactgcca
      301 accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa tgataaacat
      361 cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga agctatacat
      421 tagaaaggtc gactgtgacc ctatagatgc agtacatgca gcttatgagc atttcttctg
      481 ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc tctttctttg
      541 aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg agtaatgatt
      601 tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt caagacaagc
      661 ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat tatgattgat
      721 tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac tattttttcc
      781 ttatttgaaa acataaattt gtctttttct tcattccact cataaaagcc actcattggg
      841 aaaacacaac gattttgcaa aaatggttga tgaaaggtct ttttttcttc tactgtctct
      901 gcacgggcat tgatcatcaa ttgtcctttt ttaaatccgg taaatcccca cttcatgatt
      961 tccggagtga ggatgttttc ttcctctttt cttaacgtca caacaagatt tgacggaaaa
     1021 acttctccag ctgctaattc tgctgcctgc tccggttgtt tcttctgggc ttctcgataa
     1081 taattctgaa ggagctctga ctctagatca aaaaaataac gaccacacat cctaatccct
     1141 cccaaccttt ttttctatca tacactgcaa caagaagttc gtaccaaaaa aacgctttca
     1201 atttgtgtag aactaaaaaa attgtgacaa gtgacaaact ctccagtgaa tggtattctc
     1261 ctgccaactc cttggtcaca agtcacaata ttcaacaaac atgagaagct attttttgaa
     1321 attgttcctt gtgacccgaa gtgaaacggc aggcttacga cctctgggta aacggtgttc
     1381 cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaatttgaga agcaattttc
     1441 gtaaatgcct tcttatttct tgaagctgac cacttctgtc acaacctcta attattaaag
     1501 aaccttttgc gaagttaaca atctttcttt gatcaattgg aaattctgtc taagtagtga
     1561 tagatcttgt gtgactttca acaataagat actgccctgg atttcagcaa tcaacgcttt
     1621 ggcttgacgg tctgcctctg catcggacag tttttcttca gaaagtgcca ggatattttg
     1681 tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt attcttcatc
     1741 cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag tgatctgtgt
     1801 ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg gctgttttcc
     1861 agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg catggatcac
     1921 cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt ttcctgtatc
     1981 agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat aaataagatc
     2041 tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt ttcatttctc
     2101 atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt aaaaaagact
     2161 gtgccaaatt atctgacaca gccctcatta taaaactgtt ctcatatagc tagatgttca
     2221 ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg ctctaatact
     2281 tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc tgcatccatt
     2341 gcttcttgac caggagcacc aattgcatct gtcgctacca caacattgaa tccactctct
     2401 accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc gagaattact
     2461 gtgtcgattc cattttttct caattgaagc gctaagtcgt tgctttctgg tccaaagatt
     2521 ttatgaggag agctgatgat cgtattttca tccaaatatg gtttcaattc ttcgatgaaa
     2581 tcagctcctg aaccttcttc aactgattca tattgacttt tcctagcaaa catctgctgt
     2641 tcaagcatca tgtgttctcc tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa
     2701 taatgaggag aaataaagag tttgtatcct ttttcttttg cttgtttcac catttcgatc
     2761 atgttttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa gccaccgcct
     2821 gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt tttattattg
     2881 aacatggtgg attctccatt cgttatttat tttaatacat catttaaagc ttcactcatt
     2941 gttggatgag aatagatttg atctctcaat aacgtataag gaagttttcc attcatagct
     3001 agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc ccctagaatc
     3061 aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc taatacttgt
     3121 gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc ttgtgctttg
     3181 gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac actgtaaggc
     3241 acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc cataatgatt
     3301 cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc
     3361 caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc gcgatctgtt
     3421 agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct tccagttgca
     3481 gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa ggtgacggca
     3541 gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa ttcgattccc
     3601 gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg aggaagaaat
     3661 tcaggatggg catcaagaac gacaacttta gatccgtatt cattgaacat tgatgcaaat
     3721 tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg ttcctgatcc
     3781 atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat tggtaaaatg
     3841 attggttcag aaccagtatt gatgaaaaac cgttcgcctt caacaatcac tttttcacca
     3901 ttattcgtca tttcgatttg atggttagaa agaaaacgtg ctgtaccgtc catcactgtc
     3961 ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc cgtcaatttt
     4021 tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa aatcaatgat
     4081 ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt tgacttttca
     4141 acgactaaaa ccgattcgcc ttttgaagct aacgccttcg ctaaagtctt accgcccttt
     4201 ccaaaaccga tcacaatatt ttgatatttt tccatccttt ttccctcgct tcttttttac
     4261 ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa tcatctgctt
     4321 tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc tggttcgatt
     4381 tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag ttgctttgga
     4441 gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt cgctgatgcc
     4501 atcgatatgc acttctaata ctacaccgat ctgatagcca ccatcatcta aattataaag
     4561 agagacacgt gcttttactg tggactctgc tgtcatctct gcttctttaa tcatgtgctg
     4621 tactgcacca ttgaaacaag aagcataacc tgctgcaaat aattgttctg gatttgttcc
     4681 ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg gtgcctcgac
     4741 ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt tcattgtact
     4801 gctcctttcg attgaccatt tgctacttgg ctataactat agtgtatgac aagtaaggcg
     4861 ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact atttttccaa
     4921 aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa
     4981 tcttttctac ataatctttt atatgattga ttttaagata catatatacc agaaagttga
     5041 ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt tcctcctttt
     5101 gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt ccaagcaact
     5161 agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga aaaatgtccg
     5221 aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc attttctatc
     5281 tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc gtttccgtat
     5341 ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt catccctttt
     5401 tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc gcgtggttca
     5461 tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa ctctttgatt
     5521 tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag gatctttgcg
     5581 atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg tccgcttgtt
     5641 tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt attgattaca
     5701 tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat gaatctttgt
     5761 agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc ttttaatacg
     5821 attgtactta atactgcgat caaattattt cgattgatca ctgttccgga agcactgaca
     5881 actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa
     5941 acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat atgattagga
     6001 aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa gctattcaat
     6061 actttttcag caaagaatcc accagcacca tttccagcat ccacgatgat tttccaacct
     6121 tttagtggtt tgtcatcttg tgttcccatc ccttttttga tttttgtgac taaatcttgc
     6181 gcataacgtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct ttttattttt
     6241 tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc tgaggtaaag
     6301 aattttattc cattataata atagggaaga tggctagcag tcagcataac cgcagcatcg
     6361 cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc taattcgaaa
     6421 tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt cagtgctggt
     6481 ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg attggttgac
     6541 ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac catgtctgtc
     6601 gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc cgatccattt
     6661 tgtagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc attttttcca
     6721 gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg aatgtatcta
     6781 caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa ttcgttacca
     6841 catcggcacc atttcgttca gtcaatacga tgaccatgaa ctgaacagac tggatacgca
     6901 aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca gatggctggt
     6961 taaataatgt atagcgtact attttgtttt cattttgact ttttactaac ttcatttctc
     7021 gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa
     7081 gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc
     7141 gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag ataagtaata
     7201 tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg ttgctaacct
     7261 ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt tcttggatcg
     7321 ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt tgcgataaat
     7381 gcttctaatt tctcaaacgt tgctggttct tcgtcatagg gcccgacgtg taagatttgg
     7441 gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc ttccggaagt
     7501 ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg
     7561 atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa atacttttct
     7621 tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc aggaatcagc
     7681 caattttttt taggcgacat gcgtattgca tagctgactg catatagaca ggctactctt
     7741 cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa tggtttttta
     7801 ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt acgccattca
     7861 attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat catacctctt
     7921 tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag aataagttaa
     7981 gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg tttctatttt
     8041 tataatagaa atcactctct ttttttacga aattttttag aggtgatgta aatgacaaaa
     8101 caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc ttttgcgggc
     8161 gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca gtttgattta
     8221 acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc gatcattgtt
     8281 ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt agcttcaaat
     8341 ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat ggtcctattg
     8401 tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat gttccatatt
     8461 atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg tactttgaca
     8521 acggccattg ctccagcaat tggtcccact tatggaggca ttatgacttc acattttaca
     8581 tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat tatcggctgg
     8641 tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat aagcgtttta
     8701 ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg aacagtttcc
     8761 agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta tcgaagtaaa
     8821 aaagtggagc atccgctgat ccgactgtct attctttcaa ataaaacatt ccgcttgttt
     8881 ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt cttgccgaat
     8941 ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat gcttcccggc
     9001 gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca atatggtccg
     9061 aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc attattccta
     9121 ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta tatgatcggt
     9181 atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa tgaaaaacta
     9241 caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc tgtggctact
     9301 tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa ctatgaagtg
     9361 tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc
     9421 tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc atatacggta
     9481 ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta
     9541 tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc agttacttag
     9601 atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca actaaagcta
     9661 gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg
     9721 ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt gatcaaacga
     9781 tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct gaattggctg
     9841 ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa tggctttcac
     9901 ctgaacaaac atctcaagca cttccgttat tgaaaccagc agtttcactg cgtatcagtg
     9961 gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt ttagcacaaa
    10021 aacaaatgga gcagatccag gcaaaagtca cttcgcataa gaagagcgat tcttggctca
    10081 tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct ggcccggcac
    10141 ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa aaagggcagc
    10201 tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct atgcttgacg
    10261 gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct acccatgaaa
    10321 acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg actaagagta
    10381 cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag ttccgtgtag
    10441 gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca gatgatccaa
    10501 gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct tatatcggct
    10561 atctattagc caacttctta aatactggtc attggcctac ggaagattat caaaaggaca
    10621 tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag taaggatccc
    10681 gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc aatttctgaa
    10741 tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct atctgagctt
    10801 caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag gacgtatcct
    10861 caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat ggaccaaacg
    10921 acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt
    10981 tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt tgctgtttca
    11041 aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg aaaggattag
    11101 caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt tccgcgtctg
    11161 atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aaccgggtca ttgatcttga
    11221 taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc ttttcgctaa
    11281 gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca tttccgtttc
    11341 gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg cccacattct
    11401 gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt gttttatcaa
    11461 agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg aaagcatcgt
    11521 catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc gttgattttc
    11581 cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta gcatattgtt
    11641 tcatcccacc gtggcaggca ataaaaccat tgcgatttcc caatgcccaa gcaagcgtca
    11701 aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct gtattatgga
    11761 gagcatcaaa caaaatgagt ccattgggaa atgtttcatg tgtccactcg ggatcacaaa
    11821 agatataaag atctccctca tcaaaggaaa cagacttttg ataagtcttt aagatcttcg
    11881 aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat ccttctggaa
    11941 tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact tttgtatggt
    12001 aaaactggcg tttgcgactt tgatacacag cttctcttaa aattttctcg tatttgcttt
    12061 gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc acaatgcttc
    12121 catcattgaa tactagtgtc ctgacatcag ctggtaaatc tagttcttca gcatgtgaga
    12181 ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa gctgtcttcc
    12241 tgtcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac
    12301 tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct tttgtactca
    12361 tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt attcgagttt
    12421 ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa aaaaaccgag
    12481 aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt agtaccagcc
    12541 gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg ttacatattg
    12601 ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg tagcatctaa
    12661 ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg attctttttg
    12721 tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcaactg gggcagcagg
    12781 agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt
    12841 tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat catttgaagt
    12901 tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg aatttttttc
    12961 agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct tagataatga
    13021 atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc ctacaaacat
    13081 tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg aattcatatt
    13141 aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat catttagaaa
    13201 tatttcatag gtaaaagaat catgacattt aatgacaaaa tgacgaatta tttaatattt
    13261 gtaa
//
LOCUS       DACCQI010000080        13145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCQI010000080 DACCQI010000000
VERSION     DACCQI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.081"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(77..149)
                     /locus_tag="IVO76_001964"
     tRNA            complement(77..149)
                     /locus_tag="IVO76_001964"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IVO76_001965"
     CDS             530..1099
                     /locus_tag="IVO76_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7429918.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IVO76_001966"
     CDS             1086..1946
                     /locus_tag="IVO76_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7429919.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IVO76_001967"
     CDS             2136..2840
                     /locus_tag="IVO76_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7429920.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IVO76_001968"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IVO76_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7429921.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IVO76_001969"
     CDS             4677..5993
                     /locus_tag="IVO76_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429922.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IVO76_001970"
     CDS             5994..6863
                     /locus_tag="IVO76_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429923.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IVO76_001971"
     CDS             6918..7727
                     /locus_tag="IVO76_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7429924.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IVO76_001972"
     CDS             complement(7830..8459)
                     /locus_tag="IVO76_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP7429925.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IVO76_001973"
     CDS             8629..9921
                     /locus_tag="IVO76_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7429926.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IVO76_001974"
     CDS             complement(9997..10659)
                     /locus_tag="IVO76_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7429927.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IVO76_001975"
     CDS             10884..11168
                     /locus_tag="IVO76_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7429928.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IVO76_001976"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IVO76_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7429929.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2264 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccatt
//
LOCUS       DACCQI010000081        12967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCQI010000081 DACCQI010000000
VERSION     DACCQI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.082"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(260..979)
                     /locus_tag="IVO76_001977"
     CDS             complement(260..979)
                     /locus_tag="IVO76_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7429930.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1028..2674)
                     /locus_tag="IVO76_001978"
     CDS             complement(1028..2674)
                     /locus_tag="IVO76_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7429931.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            2853..3443
                     /locus_tag="IVO76_001979"
     CDS             2853..3443
                     /locus_tag="IVO76_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7429932.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3535..5040
                     /locus_tag="IVO76_001980"
     CDS             3535..5040
                     /locus_tag="IVO76_001980"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7429933.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IVO76_001981"
     CDS             complement(5124..6476)
                     /gene="celB"
                     /locus_tag="IVO76_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7429934.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6476..6994)
                     /locus_tag="IVO76_001982"
     CDS             complement(6476..6994)
                     /locus_tag="IVO76_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429935.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7010..7336)
                     /locus_tag="IVO76_001983"
     CDS             complement(7010..7336)
                     /locus_tag="IVO76_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7429936.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7349..9328)
                     /locus_tag="IVO76_001984"
     CDS             complement(7349..9328)
                     /locus_tag="IVO76_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7429937.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9349..9663)
                     /locus_tag="IVO76_001985"
     CDS             complement(9349..9663)
                     /locus_tag="IVO76_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7429938.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            9831..10124
                     /locus_tag="IVO76_001986"
     CDS             9831..10124
                     /locus_tag="IVO76_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7429939.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10202..11314)
                     /locus_tag="IVO76_001987"
     CDS             complement(10202..11314)
                     /locus_tag="IVO76_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7429940.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11467..11691
                     /locus_tag="IVO76_001988"
     CDS             11467..11691
                     /locus_tag="IVO76_001988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429941.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            11701..11880
                     /locus_tag="IVO76_001989"
     CDS             11701..11880
                     /locus_tag="IVO76_001989"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429942.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12074..12244
                     /locus_tag="IVO76_001990"
     CDS             12074..12244
                     /locus_tag="IVO76_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7429943.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(12668..>12967)
                     /locus_tag="IVO76_001991"
     CDS             complement(12668..>12967)
                     /locus_tag="IVO76_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7429944.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4088 a         2610 c         2204 g         4065 t
ORIGIN      
        1 aggttgtgaa ttcagcgttt tggcctgagt attagggaga tcgtgtcctt actatctaac
       61 tctgagtcac aaggaacaat ggggactgta acaaaagtct tgtcacagtc ccttattccg
      121 aacaaacggt gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaaagcc
      181 atccgaaagt cagacggaca agtctgcttt tcagatggcg ctacaactac tctgcttatt
      241 cttttagcta tccattcaat tatttttctc tcacttgttc tagctcttct ttggtcatgg
      301 gtcgatattg ccctttattc aaatctttag gcagccaaaa ttttcccata cggattcttt
      361 ttaaatataa gacttctttt cctactgctt tgaccattcg cttaacttgg tgaaactttc
      421 cttcatgcaa aatcagacgg ataattgaag tttctgtctc ttgatctact tgatcaattt
      481 ggagttttgc cggcaatgtc tgctcatcgc catcaataat cagtccttct gcaaataaac
      541 ggatatcttc ttctgtcatt ataccgctaa cctcagctag atattctttc tccacatgtt
      601 ttttaggtga tagtaactga tgggcaaaca ccccgtcatt cgttaaaatc aagaggccct
      661 ctgtgtcttt atctaatcgg ccgactggaa aaagatcttc ccgataatcc tggtcttcca
      721 ataaatcgat tacggtttga tcatgattat cctgagtagc agaaatgact cctgcaggtt
      781 tatgcaaaat gtagtaaaaa tctttttgat acgtaatgat ttctcctaaa taagtaaccg
      841 catcttcgaa ttcatttact tgcgctttat cgcttttcac cacttgtcca ttgacttcga
      901 tcgttccttt tttgattaat cctttgactt ctttgcggct gccaagtccc atatctgcta
      961 aaaacttatc taaacgcatg cgtttccccc atttcaaaag agcatcaatc tagctcttct
     1021 gattcactta ttttattttt aatttatgtc gtaaacgcgc tattcctggt cctagtagtc
     1081 tttctgccaa gcggattttc aaagctaaat aaccatacac taaagcccct atacctgcta
     1141 ctacaagaat caacaataga gactggaatt tgctatcttc gtttaaaaaa gcaccaaaaa
     1201 tcatctttgt gatccacgaa gccaaaagca taatcaaggt aagaataaag atcagcaatg
     1261 tcctgcgcca gacaaaactt ggtttaaagc gggctacttc atggattctc tttaagatca
     1321 aggtacaaga cacagcaaat ccgatcattg tcgccaataa agggccgtat acttcaaata
     1381 aacgaatggc tgggtattga agaaggagtt tcactagaaa cccaataacc aaataattaa
     1441 ttgccgcctt gttttcaaac attccttgca gcatgttgga aactagcata tatagtccta
     1501 ggaagagtcc tacaaaactt gcttggatca gtacattact tcctagagaa tccggtgtat
     1561 aaaataatgt atttagcgga taggcaagta acataacacc aaatgtcgcc ggaaacataa
     1621 aaaatgaaaa caactgtaaa tttccgcttg tcaattttgc cagacctact cgatctttca
     1681 acgtaacagc ttctgtaatc aacggtaacc cagtagacgc gatcgatgtt gccaatgcaa
     1741 taactaccat cgtcaactta tctggattgg cactaaaaat cgaaaaaaga tccagtaact
     1801 gtgcattgga atactcggtg gatgcagaca tgatcttcat aaaagtgaac tgatcgacta
     1861 atttgaagat cgttacccct gaacccacaa taataaacgg aatcgcttct ttaacggtat
     1921 ctaacaacaa ttccttcgtt gcaatattta ttttgttttc gctgtagtga ataaacgccg
     1981 atgtatacgc ttgatgtttc ttcaaaaaat acaaaaggac aagaatactt gctagcatac
     2041 cgataaatgc cgcgaaagtt gattgcgtga ccgccgtcac atagtcacca tccatgactt
     2101 tcatgatgat gaatgtagcc aatagcatat aaaaaatacg tgccacctgt tctacaatct
     2161 gcgacagcgc ataaggcatc atttcttgat tgccttgcaa ataccctcgt atcacactca
     2221 tacaaggaaa aaccaataca gcgatactca aagaacgcat cgttggaatc agttcttctc
     2281 cgccaccaga agcatgagct agccaaggag aggcgatgta catgaacaaa gcaaacaaca
     2341 ggcctaaccc gcccatcatc tgtaatgcac ggataaataa tcgtcgtgaa gtgccatatt
     2401 cattcaatga attatagcgt gcagtttgtt ttgcaatcgc agcaggtatt cctgctgtag
     2461 agatcagtag aaacaatgca tatatattgt atcccatatt gaataatcca ttggcagcct
     2521 tagcattttc gcccatccaa gcataccagg gaatgatata gacagcgcct agcaatcttg
     2581 aaatgatatt actggcggtc atccaagcag agcctcgtgc cattttttct tgcggtgtta
     2641 attgatctaa tgctggtttt ctttgattat ccatcggtca gattccaact ttctacaaaa
     2701 cattactatc attttaattg gtaaaacaga aactggcaat cgattttata taacttttat
     2761 aagaacaatt gtttttttga attcggtttc gtttttttgg taaagtaaag aaatatacag
     2821 ccttttatgg ctaacattta taggagatat caatgacaca agaaataaaa aaactacccg
     2881 ttatattttt aggtcttttc tttatcacag ttggattgaa ttggttcttg ctccctcacg
     2941 atatagcttc cgccggagtg ggcgccatcg gacatttagt tgaaacggcc ttttctatcc
     3001 aacgctcagt tactgtttgg agcatcaatc ttatgatgct gcttctagcc gctgtcctat
     3061 taggaaaatc agtatttttt aagactgtag cgggaagtct gctttttcct gttgttttag
     3121 agattgttcc aaaagtggaa atgatctcct cacatttctt ttcactgatt tcaggaagtc
     3181 ttttgttcag tcttggggtt tacacattgt acacagtcgg ggcctcaaat ggcggtataa
     3241 cgataccgcc gattattttc cacaaatttt ttcggcttcc aatgccgcaa ggacttttac
     3301 ttaccaatag tcttatcgtc tttttgaatt attatgtttt tggattgttg gagacactat
     3361 tcgttctcct atctattaca gtcagttcat tgtttatgaa aatactcatc cgcttgtccc
     3421 ctgtgtcaag aaaatttgaa taaagctgga ttttgtcata gcggaaacaa acgatatatt
     3481 aaaaagagtg tgctataata aaccaagaaa ttgaataaag cgagggatca atcaatgagc
     3541 ttctctttag aagacatccg tcatttacta ttaaaagaaa atctattaaa agaatttgtt
     3601 tcaacaagag gctggcatct tgatgtacca gaaaaagctg aatttcttca gttaagttat
     3661 gattcaagaa aagctgatgc ttctactttg tttttctgta aaggaatgaa tttcaaagaa
     3721 gaatatttgg attcagcaat cgaacagggt attcaatatt atgtatcaga acaaccatac
     3781 gaaaattcag cagtaggaat catcgtaacg gatatccgta aagcaatggc gcttcttgct
     3841 atggcttttt acgactatcc gcaaaataaa ttgaaagtga tcggattcac tggaacaaaa
     3901 ggtaaaacaa ctgctgcgta ctttacaaaa gcaatcttgg atcacacaac aaataaaaaa
     3961 acagcattgc tttctactat gaatacaacg ttagatggca aaacatattt caaatcccat
     4021 ttgacaacgc ctgagtcatt agatttatat cgtatgatgt cagaagcagt tgaaaatggt
     4081 atgactcatc tagtgatgga agtttcttca caagcttaca aaacacaacg cgtatacggc
     4141 ttgactttag atgtcggaat cttcttgaat atctcgcctg accatatcag tccgatcgaa
     4201 catccgacat ttgatgacta tttctattgc aagcgacaat tgatcttgaa ttcaaaaaca
     4261 gttgttttaa atcatgaaag tgactatttt gatttactta gggaaacagc cgatctattc
     4321 cagattccaa cgatcactta tggtcgttcg gaagaaagca attatcaagt gatccgttca
     4381 gataaaggta ctcacggttt tactttatct tctcatgaag atcgcttagc tatctgcgat
     4441 actgcttatg atattttgct ggctggagga ttcaaccaag aaaatgcagc cagtgcgatc
     4501 attgccgcag cactaacagg tgcctccagt caagatgcac aagacggatt aaaagaagct
     4561 cgtgtaccag gccgaatgga tcaattgatc caatccaatg gagcgcatgt ctatgtggac
     4621 tatgcacata attatctaag cttgaaaaca ttgctgaaat ttgccaaaaa tgaacatcca
     4681 gatggccgtg taattgttgt attaggcagc ccaggaaaca aagcaatctc ccgtcgacat
     4741 gattttggaa aagtgctatc agaaacagct gacatcgcct ttttgacggc agatgaccca
     4801 gcatttgaag atccgaaaaa aatcgcggaa gaaatcaatg aagcaattac taatccagat
     4861 ttgatcgttc attatgaaat ggatcgtcct gaagcgattc gacgcgcttt agcagaaagc
     4921 gctccgcaag attcagtcgt tattgcggga aaaggcgtgg atccctatca aaaaatcaat
     4981 ggtgtagatg aaccttatga aggggattat gcaatcgtga agcggttgat tgaagaataa
     5041 tggtatttat atactagtgg tttcccattt ctaagaaaaa aagggactgt gattttttgt
     5101 cacagttcct tttttcttag aaatcatttt aacttttcac tagctaattc tgctgccttt
     5161 tctctttctt catttaataa tttattatca tagaatttga taaatgggaa ccagataatg
     5221 aatgcgatga ttgcacaaat gactgctaac acagcagctt tccaatcacc accactacca
     5281 acaaatgctc cgatcccgac tggtgaaggc cacggcatct gtgcaagcat cggtttgacg
     5341 atttccagcc gaatggcaaa ataagcgagt gtagcagaag ccatcggagc taagaaaaac
     5401 ggaaccgcta aatagggatt gtagacgatt ggcatcccga aaatgattgg ttcattgata
     5461 ttgaataatc ctggtacaag tgaggctttt cctaacactt tcagctgatc agatttcgcg
     5521 aggaatgcaa taaagatgat caatcctaaa gtcgcacctg atccaccaac ggttacataa
     5581 gaattgttga attctcctgc caacggataa tttgcgcctt gtgcgttagc tgccatatta
     5641 gctaatacga tcggggtcaa gaaagaagtg atgatattcg ctccatggat ccctacgatc
     5701 cacaatgcat ggatcaagaa ataaatcacc atgacaccaa gccatgtatt cgtcaactgc
     5761 gtaacaaacc caaatgggat agcaatcatt ttgaagatat ccgttcctag tgcaactaat
     5821 aatccattga tgatcatcac cacaaacgca atgacaaatg ttgggatcag agcagtgaaa
     5881 gaacgagaaa ctcctggcgg aacaacatct ggcattttta tgacccaatt tctcttgaca
     5941 cacatacaat acaattcgac cgcaataatc gacataataa tagctgtaaa gattcctgaa
     6001 gttcctaaac gttcgacaaa agagcccatc ctaataccat ttactacagt ttctgtctca
     6061 ttgatagaag caatcaagct gattttacca tcagtcatga tcaattcagg gatacacata
     6121 aagaatgcaa aaacagatag taatgccccc gtgagcggat taacatttaa tttttgttca
     6181 tctcgttcaa ttgacgttaa ttcataagca aacacaatgg caaagtaaat agataaaatc
     6241 cccatcgtca ttgtattgac gatcatgtac aagtcactaa ctttgaaaaa agttgcatta
     6301 aaaaatcctt ctaaaaatgg gaacggtaat ggcaaaatat ttaaaaccaa aaacatcgag
     6361 cctacaatcg tgaaaggaat agaagccatc cccgctgcca tgacagcccg aacgatttta
     6421 agttgcgcaa ttttccccat aggtcccatc aggtatttct gcaaaaaatc aaacattagt
     6481 tattctcctt tcctaaatat aacgcttttt catattgttt aatacggata tactgttggt
     6541 cttggttgag tgaaattttc tttaaacgat actgtataaa tgcactggca aaaataccgc
     6601 ttacaacaag aatgaaaaca aataactgtc ctccattgtt atttgccata aaaggataca
     6661 agaatgtaaa tcctgaggta aacaacactg gaatcactaa taaattaaag acactctgga
     6721 acaaaaaata ccactttgtc catggaacgt tcgtttggtg ttttcgataa agctttactt
     6781 gttctcctac tgcaggcaaa atagctaaaa tcagaatcat cggtaaacta cttcctaaac
     6841 ttttagctga tagaagcaga atcatccatt gcatgttcat gaaaaagaaa aaggcagtca
     6901 ggtaacgtac gattagataa cgattgaaat acatgttttt caaactaagt tgcttttttt
     6961 ctaattcgat tttccttttt actgtatcct gcacaatgat ccttcctttc tatttatttt
     7021 tttgatacaa ataattcatt tcctgtgcaa cttccaataa tgtcatcgtc gtcatcagat
     7081 gatcttgtgc atggaccatg ataatttcca tttctatttt tgtcccgctg gcatattctt
     7141 tcagaagttg agtttgtgac tgatgcgcat gaagaatctc ttcatttgct tgtcgcaact
     7201 tctcttctac ttcttcaaac atctctttac gcatcgagcg aaatgcttca tgtatcagtg
     7261 tccgtgcatt tccgctatgg aaaatgatat caaaagaagt cacttgtagt gtttcactgt
     7321 ttattttttc agccatctgt tttcctcctt attcgatcag tttgataaat ttactgctga
     7381 aattctcaaa aataggttca gctaagattt cctgctgtac ttttagctgg tcaactaacc
     7441 taacgattgc tttagtcacg accgtaattc cttcattttc gacataagaa ggcgaaacta
     7501 aaaaaacaaa atgaatttct tgatattgat cccactgcat cccagaagga atcaatgcaa
     7561 cggctacctt tgtttcgatt ccaactggaa cagcgggatg tgggactgcc actgtatcac
     7621 tgaatatgat ttgtcccata ttttcacgtt gctggatctg ctttcgcatt tctcttatat
     7681 atccttcttc ttcatttatg ctcagctttt gcagcaaatc atgaagaatt tcttcctttt
     7741 tcggttcgta cgtataagtc ttgaaaaatt tttcagacaa ctgttcggtg taaatctgct
     7801 ttttttgctg tagagaaaat gctggtatcg gtctatctga ggaatgatag ggtatagatt
     7861 ctccaactac ctttcgtatt ttacgaacat catcttcatt caaaaaaata ctgacatgta
     7921 aaactggaat tttgaaaaac attgtcgaaa gatcaataga agaaataatc aggtcgattc
     7981 cttttaaagt tgtttcattg atctcataat agccttttac acttaccact gtaatatttt
     8041 taccgaattc actgactacg cgattcttta atagttgggc actgccgtaa cccgttgcac
     8101 agataatcaa tgcatgtagt ttatgcgcag ctctttcttt ttccaatgcc gccatgagat
     8161 gcaaagccaa ataggcccat tcatcttcat tgatggtata gtttttcaaa gccggcatgt
     8221 tgctgaaatg cctttttact gcgtcaaaga ctttgggatt ttctttcttt atctcctcag
     8281 tcaatgggtt ctcaatgcga atacctcggc tcaatcgaat catcatcggt gacaaatgat
     8341 cgagtagtcc attgaaaagc tggagatcct ctactaatga aaaatgaaat tcctgagaca
     8401 atcttttcag aactttcgct aattcctttg atagttcctg atcattttga ttttctttgt
     8461 gattcgactt agccatgaga tgtaatgtca aataagcaat ttcttcttcg ggaaacgata
     8521 cacctgcgat tacctcaata cgttgaacga tccgctgcgc cacttgatac tctactcgct
     8581 tagaaacttc ttgttcgatg cctaattcct tgatttcaaa cccttctcgg agccgtttga
     8641 tccccaaagc caagtgtaaa gtcagattct gtatgataaa atccgaaact tttaacttag
     8701 cttctcttgt ttcatcgaga atgataatga ctagctcttc aaaactgatg tctgggaaaa
     8761 aacggctttg ccctaaatat tctttcaatg aatttccata atggtttcca aaaaaagtat
     8821 ccatgataaa atgccgtcga tttctttctt ccccttcgat ccatgtccct tgaccatgct
     8881 tagaaacgat ttttagtgaa tatggaatca atcgctcctt gatttcttgc atgtcctttg
     8941 tcaaactgga acgactgata taaagctctt ctgccaaatc atccataaaa agaatggcat
     9001 cttccaaaag caattggttc aatatatact tttgtcgatc ttcggactca taaaatcctt
     9061 gcggttctct agcatatcca ggtactattt cttgccgact caaaaacaag tcaaatgtca
     9121 gtctttgtaa aatatgcagt tggtagccat aacctggttt agcaatgatc ttacctccgt
     9181 ttttttctac taactctttg atccgctgca agtaattacg gattgtccga tcagataata
     9241 acaattcaga agctaaacgt tgactggtga catactcctt cttgtgatca atcaaatatc
     9301 tcaacagcaa tttttccttc tgtttcatta tcatccactc ctattccatc atatgttctc
     9361 ttcgatcacc tctgctaact tctggatgcc tgtagggatt gggacatacg cttggaaagg
     9421 gatactaaca acaggctttc caacagattc tcctaatttt ttgaatttat ccaaaaacat
     9481 cgtcgtttgc gggctgatca gaaataaatc aaaatcacta ttcttaatca tattatcgcc
     9541 ttctgtagct gaaactgcat ctacgagtac ctcttctctt ttttgcttaa agaaatccgt
     9601 tgtctttgag gccatcatcg aggatgacat tcctgccgca caaataatca atgctttttt
     9661 cataaatcaa tcactccttt tatttttata aaccaagcat atcttgtaag cgttttctat
     9721 tcaaaatata tatttccgta gcattgcgga aaaaatgtgt atcatatctt gaataataat
     9781 ttctggcacc gtgatatact agtgatagaa ttttttcagg aggggattta gtgagtatca
     9841 cagtgaatat tttgtataca agtacaaatg gacaagccag aaaatttgca gaagaaatgg
     9901 tcgaaagaaa cattgtcaaa gcgattcgtg cagaagaagg aaacgagaaa tatgactatt
     9961 actttccact agaagatcca gaatctcttt tactgatcga ccgttggaaa gatgaagaag
    10021 caatcgaaaa gcatcacaaa tccgaaatga tggctcaaat aaacgaactc cgtaaaaagt
    10081 ataaattaaa aatgaaagtc gaacgtttca ctgatttacc gtaaaaagat acacatggaa
    10141 tgatagcaat ttatcatcca tgtgtatctt cactttttcc ttatttttta ttcgatctca
    10201 tctaatgatt caaaccgtgt ttttctctta tttttcggat aagtatttca gtcaccccaa
    10261 tatacatgat tcctaaacat accccaagga tattctcaaa aatgcggatc gttactgctc
    10321 ctggaacccc gaataaactg gcagtgattg ccaaagcacc aaaagaatta aaaactgttt
    10381 tatatctata ggtagaacaa acacctaaag ctagaccacc taatataccg acccagttaa
    10441 aaggaataaa ttggctgata ccaataaata aaacacatcc aatcaatgag ccaatgattc
    10501 ggtctactgc tcgttctttg aaaccaatcg acatcaatcc atagctagat acaatagaag
    10561 aaaatgcaaa ccctgcccac atcaatcgct gaaaagggac atactctcct gcaacgatca
    10621 aaagactaat gccaaatgca tagatgagca tccaaatttt ttcctgcgaa tacatgcctt
    10681 tcaaaaaaat ctctttaaac aagcttttgt ctttgttctt ttctcgatgt tttctgtaaa
    10741 ggagaactga aaaccaacaa aaaaacaaaa ataataagct acttgtttgt gtaaagaaat
    10801 tcttgttcaa cgaatcttta ggtaacgaat aaacaataaa gagatatgaa aagccgtaca
    10861 aagaagcatt ccccattttg ggatcactag cggttgccat taatatgctg cttagtaaaa
    10921 taaaatgaat caacatgccc ataactgacc atttgatcag ctggacatac ggtgctacta
    10981 atagtgagaa catgacgatt gctaaaccta tgatcgaatg ggaaatctta taaccaaagt
    11041 caacaaaacg aatactcatc aacatacaga ataaaaccac agcgtatggt ttatgcttcg
    11101 attcaaataa tgttgtaatt ataatgataa aacaaatggc aaacaatacg atgaataatg
    11161 atcgtaaaaa caatgaacaa atatacatgc acttttcttt tttagaagct gcttctcgta
    11221 ttttttgttt taaaataaac ggatctagtt gtagagactg ataaaaatcc acctgttgtc
    11281 gtcctttctt tttactttta ttttttacag tcatctatga caggttgtaa atactatgga
    11341 acatcatatg gcgttgtcat tttcttgtca actatttttc ccattcatgt ttttataaaa
    11401 aaacatatcg ttttcttgta tgattaaatc accacaaaat aaacaactga gagaacgata
    11461 tgctttatga atggttctat caaaaaaatt ctgagatcaa tagacaaaaa tttgatgatt
    11521 acagaggtct cttacgaaac cttacagaag aaaaaaacat tgatcgttgc tgcagttctc
    11581 ttccctgctc catgtgtctg tagaaattgt ggttctgctg tggtagatgg aaataggaaa
    11641 ttaatggtgg tgaaaaacaa gaagaaggaa accattgtcc actttgaata atataatcat
    11701 atgccaatgg ttatacgcct aaaaaagcag cgctatacct gtaaaaactg tcgaagccat
    11761 tggaacgctc aaagttattt tatccgtcca aggcactcga tttcaaatca tgtcagacat
    11821 aaaatcactt ctttactgac tgaaaaagtg tcgttatttt ttatttcgaa aagttgttag
    11881 gtatctttga caaccgttat tcgcacattg aaagagttga gaagctattt accaaagtct
    11941 tccaaaagaa ttctgtcttg agtgttgagg gttgtgaatt tcgttctcac gtttccgttg
    12001 aggataaact gagttttatt tccgcggatg gcgagacaga aaaattaatc gagaaaattt
    12061 acctacacaa aaaattcctc gattaacaaa ctatttcctt agctgcacta atcctaaaga
    12121 aatagaattc ttagttacag acatgaacgc cacctacttt cagctcacca aacatgttct
    12181 accaaatgcg aaattggtga ttgatcgatt acttggtttt tccacgcctt tcaaggaggc
    12241 gtaaccttta ttcacgagtt ggttgaagct ttccgagaca aaagacccga cttgtttttc
    12301 tccttattga caaagagcca ttaaaactag agaaaaaatt acaactgatc gctgattatt
    12361 tgtagaaaga aaatcaaagt attacccaat aaacttgagt cattaggcaa aaaacactgc
    12421 gaaactcaag aatgattttc gcagtgtttt tgtatatttt ttattaacta gtattactac
    12481 aaaggtttat ttttcacttc tcaacgcagc acaaaattgt ctttacttat ttactggttg
    12541 ggttcaacct tctctactcc gatactaata tttgtttgta taccccttct aaaatcggta
    12601 gtttccattc aattgatggg tactagttga ttttaaaaac tatgcttatt caatttttta
    12661 ttttagctca tatatttcgt ttctttctaa attttatact gatgaaaaca agtagcacac
    12721 ccaatattga caatataaac tggctaatga tggtttctcc agtttttgga agatgcttat
    12781 ttccactgct tgttggattg tttcctctta tatctgagct atcagcaact tttctattct
    12841 cttttgttaa aaagatcgcc acttcttgtt ctttttcgat cttgaaagtt actggtgtcg
    12901 catccaattc ataccccgtt ggcgcttgag tttctaccag ctgatatgct cctggtgcta
    12961 agccatc
//
LOCUS       DACCQI010000082        12731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCQI010000082 DACCQI010000000
VERSION     DACCQI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.083"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(20..325)
                     /locus_tag="IVO76_001992"
     CDS             complement(20..325)
                     /locus_tag="IVO76_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7429945.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(350..781)
                     /gene="ruvX"
                     /locus_tag="IVO76_001993"
     CDS             complement(350..781)
                     /gene="ruvX"
                     /locus_tag="IVO76_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7429946.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(778..1044)
                     /locus_tag="IVO76_001994"
     CDS             complement(778..1044)
                     /locus_tag="IVO76_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7429947.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARSLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1299..2024)
                     /locus_tag="IVO76_001995"
     CDS             complement(1299..2024)
                     /locus_tag="IVO76_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7429948.1"
                     /translation="MSKKTTKAIVGIVAAVIAGTFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2385..2455)
                     /locus_tag="IVO76_001996"
     tRNA            complement(2385..2455)
                     /locus_tag="IVO76_001996"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2421..2423),aa:Gly,seq:ccc)
     gene            2572..3324
                     /locus_tag="IVO76_001997"
     CDS             2572..3324
                     /locus_tag="IVO76_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7429949.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3340..3819
                     /locus_tag="IVO76_001998"
     CDS             3340..3819
                     /locus_tag="IVO76_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7429950.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4070..5650)
                     /locus_tag="IVO76_001999"
     CDS             complement(4070..5650)
                     /locus_tag="IVO76_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7429951.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFIFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5643..6386)
                     /locus_tag="IVO76_002000"
     CDS             complement(5643..6386)
                     /locus_tag="IVO76_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7429952.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6562..7338)
                     /locus_tag="IVO76_002001"
     CDS             complement(6562..7338)
                     /locus_tag="IVO76_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7429953.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7586..8422)
                     /locus_tag="IVO76_002002"
     CDS             complement(7586..8422)
                     /locus_tag="IVO76_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7429954.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8433..9362)
                     /locus_tag="IVO76_002003"
     CDS             complement(8433..9362)
                     /locus_tag="IVO76_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7429955.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9554..9907
                     /locus_tag="IVO76_002004"
     CDS             9554..9907
                     /locus_tag="IVO76_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7429956.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9981..10637)
                     /locus_tag="IVO76_002005"
     CDS             complement(9981..10637)
                     /locus_tag="IVO76_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7429957.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10863..>12731
                     /locus_tag="IVO76_002006"
     CDS             10863..>12731
                     /locus_tag="IVO76_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7429958.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQ"
BASE COUNT         3965 a         2501 c         2220 g         4045 t
ORIGIN      
        1 tttctcctag ctttatagtc tattcttctt cttccgccat gaatgtattg aatacttctt
       61 caatcatgtc ccattctgcg tctgtttcga tttgttctaa ctcgccttct gttccatctt
      121 cgttttcgat gtatgcataa gcttgtaatt ctacatcttc atcttctgaa acaccagcag
      181 gatataaaag aacatagtta cgtccaaatt cttcttgtcc gtcaactgtt agtaggattt
      241 catataatgt ttcatttcct tggtcatcca ccaatgtgat gtgttcgtgt ccttcatggt
      301 cgtgggaatg gttatgttct gtcataagat tacctctttt cttttattat taaaataatt
      361 gcttacctga gccgtctaaa taattttgta agatcatcac agctgctaat ttatcgatga
      421 ctttttttcg tttggctctt gaagtatttg cctgctctac tagcatacgt tctgcctgaa
      481 cagtggttaa tcgttcatct tgataatcaa ctggcaagga gaatagttct ttgatctttt
      541 ctccgtatga catggaagct tctgcccttg gtccgatgga attgttcata tttttaggta
      601 atccaactac aaaccggtct acttcatatt gtgctactaa ttccttcaac cgatcaaagc
      661 caaactcttc ttctgcttca ttgatccgaa tgatctctac tccttgagct gtccagccaa
      721 aggggtcact gatagcaaca cctactgtct tagaaccgac atccaagccc attactctca
      781 taaattcact ccatgatttg agaggtagtc cttgacaatt acttccatga tctcatcgcg
      841 ttcgtgacgg cgaatcaaac ttcgagcatc acgataacga ggaatgtagg ccgggtctcc
      901 agaaagcaag tagccgacaa tctgattgat tggattgtag cctttttctt ccaaagcccg
      961 ataaacaatc gctaatgttt cgctcacttc ttttttgcga ctatcgtcaa aaccaaaacg
     1021 tacggtttca tctgtaaaac ccatgattct acacctcttt cccgcaaggt aattccagct
     1081 taccttctac aaccttattt tactcgaaga aagggaaaac tacaaacaaa tattccaaac
     1141 ttccgaataa attaagaaac actatttgta tgtttttttc cttttttctg tagctttatc
     1201 aaaagtactc ttttcataaa gtatacgcta tatttctttg aatgtatgtt tttacacaat
     1261 tttttcataa tattcataaa aaattcgaat agctgctact aattttttgt ttttgttgct
     1321 tgtcctgtct cataattgat cgtgtagcca tcttgaacat tgtagataaa gacattgaat
     1381 tcaatttttt tgtcttcaat gctttctgct tccaattgca ctcccctggc taccagttca
     1441 ttcccctcaa aatgaggagt gacacggtag cgtacatgat gatttgtttc tttgatatat
     1501 gccgcaatat cattttcgtg agcaagcatg tatggagcat tcaacgaacg tgtccctgtc
     1561 atcaagtttt taatgttgtt attttgacca gtcagctgga aaccaattaa atggctgcga
     1621 ttatataacc attgcccgtc acttaatttc ttttgtttcc aacctgtcgg gtcaatatac
     1681 aaaggctctc tcttttcagt agggaacaaa tcttttccaa gcatagcatt ggcttgtcct
     1741 actctgttca atttatcaat atttgaatag ctttcccatg ttcctttgga aaggctaaga
     1801 tcctcttttg taaattccgg ctgattccca ttgatttcta tttcttgatg ttttccatca
     1861 tattctggca catccgctac ttgagaagct tgtggtgtgg aatcaaaaat agttgtaatc
     1921 tgctgaaaga tatctgaatc tttttgtgtg gtggaaacac cgaatgtccc tgcaatcaca
     1981 gcagcgacga tccccacgat cgccttggtt gtttttttgc tcatttcttt actcctttac
     2041 taacagctcc ctaaatctta acatatgttc ccctataaat aaatactaga aagaaataga
     2101 aacagcaaaa aataaaacct gttttaagat ggcttaaaaa tacaaaaaag agacggattc
     2161 ctcgtctcta atcagtcacc actctatctg taaggtattt attattattc aaaaattttc
     2221 ttcatactca agacaataca ctgatttcac aaccgcttgg taaacggtgc gcaaaagctt
     2281 gcatctgcgg taataagtcc atccaagtta aaaacataag aagttgtttt tgcttggctg
     2341 ttcttattac ttgttgcaga gcagcttttt cacaacctat tttaagcggg tgacgggaat
     2401 cgaacccgcg acatcagctt gggaagctgg gattttacca ctaaactaca cccgcaatac
     2461 aaaaacagta tacgcctctt ttattccttc ctcaagtttt tttattcttt acgcttctgg
     2521 tatgttttgt agtaaaataa aactaatcaa tcaattattg gagaagatgc tatgacggga
     2581 aaagaattag atctgttgct ggacacttgt ttactcgctg ggaaaatcat gatggaaagc
     2641 aatgcggaga tgtatcgggt agaagatacg atgagtcgaa ttgctttagc ttctggaaat
     2701 ttccgtttgg tcagctatgt cactcaaacc ggtctcttta ttggtcttga tcgtacctcg
     2761 accatccgaa tggaacaaat taccaaccgt tcgattaatt tagaaaaagt agtaaaagta
     2821 aatgatcttt cacgtaaata tgttgcttca gagctaacat tagaagaact ttactgcgaa
     2881 ctgcaagcaa tcgaccgaga ccgttctttc tttcctgtct ggttgcaaat cgttagcgcg
     2941 gcagtgatca gtggaacaat tatggtgtta tttggcggaa agatgaccga tttacctgtg
     3001 actttagtca ttggaggatt gggatacgca gcttatctat atagtttgaa attttttaga
     3061 atcaaatttt tatcggaatt tttatcttct ttattgattg gcatagccgc tatctacagc
     3121 gtacgttttg gcttaggaag caatcaggat ctaatcatta tcggatgtgt gatgccttta
     3181 gttcctggag ttcaaattac gaacgctatc cgtgatttgc tagctggaca ttatgtctca
     3241 ggagtttctc gcggaacaga agcgatgatg actgctacga tgatcggttt tgcgattgct
     3301 tttattttcc agctttttta ttaggaaagg aacacaagta tggggacgat atttattcag
     3361 ttttctttta gttttttagc gactgcggca tttgccgtta ttacaaatgt acctaggcgt
     3421 tcattgattt gttgtggtct ttctggtgct tttggctgga tgatctactg gatctgtgtt
     3481 caaataggcg gatctgctgc ttttggttca ttgcttggtt ctttaggagt agcattcatc
     3541 agcgatcttt tctctaaacg tttgaaaatg cccgtaacga ttttcaatat tcccgggatg
     3601 gttcctctag tccctggtgg tttagcttat caagcggttc gaaatctcgt tactgaatcc
     3661 tatcgagaag cggctcatta tgcggtccaa gcaattatga ttgctggagc aatcgcttta
     3721 ggtcttgtat tatcagaagt ctttaaccat aacatccgta atttcaaagt aaagcgtgac
     3781 gcaatcatga tgaaacgaaa aaaacggctt tttaaataaa cgaagtgtaa caaagaaaag
     3841 gttgtgaaag agacgttcag ccttgagcga tgacaaaata tctcacaaac attgcgacaa
     3901 gtaaccgcag gagcaataag aagtaattat gagtattaaa aaaagttttg taaaaatagc
     3961 ttctcacatt tttacataaa attgacttaa tactgagaaa ctgacttttg aacaccctct
     4021 atctgtaaaa aaggggctgc gacatgattt ttgtcacggc ccctcttctt tattcaaatt
     4081 ttttccaaaa cgttcgttga taataaacga tccatagagc ggaaagtatt agaactaaac
     4141 cgatcacagg aatattcact aaccaaaatg gataaaacaa agccaacatt ccgtataaga
     4201 cgataagaat aatactgatg atcaatgaac ccatcatagt tgcgactaaa cccaaacttc
     4261 ctgaacctct gttaaataat tggttaatat tggtccagtt cagcagcagc aaccgataat
     4321 ctctagcgaa ataacgtagt gacaataagt agcagccgaa taaagaacca aatagcaagc
     4381 tagggataaa aaacaaaggc agctgaaaag acagtccgct taataatgcg atcccaccag
     4441 ttaaaaaact ctgcaatatc catccgaatc tgaacttttc ttttaaatat tggttcatag
     4501 agattggcaa agaacggata aacagaaaat tttcttgatc caaagaaata agattgctga
     4561 taaaagaagt ctgattaact gttaagaaag ccaatgcgat ccctaccagg aaaacaacac
     4621 cgattaactt attatctaaa tcagttaatt ttatttcgcc tcctagggca aaagcaagaa
     4681 taaaaatgat tggagttaac aaggaagaag aaagaacttg cataatcaaa ttaggttctt
     4741 ttactaattg agcattgtaa ctaaataaaa gctgatgcac attttggttc tttttatggg
     4801 ttctttttat tttgaactca ctgtttgaat cagtcagttg ctctgtaagc tttggtacta
     4861 tcagctgttt tacagcaagg aaaaatccca gagccgtgat agctagtatt ccccaaccca
     4921 ataaaccttg ttgagaaagt ggctgactgg cagcatcata gaatggcaat aatatcgaaa
     4981 tcggatgccg atctgttaac tcattttcaa tagtccccgt ttgatgattc atccacatga
     5041 ttccagcaaa tgcgatcagc atcgagctgc caagcaagat actagaaact gtttttctat
     5101 gttttttaaa cactgctgtt cgagccaaac caaaaacaat caaactagcc agacaaaaaa
     5161 gaataatcaa aaatagagaa aacaaaagga ttgagataat gatagtaagc aaaatcccta
     5221 gatcagctcg ccatccggtt aaaataaaca tgactagaag cggaaaaata aaaggaatga
     5281 tggtcaaagc aactaccaaa atctttgctg aaaaaacctg ttcttgtcca aaaggcagag
     5341 gcaaaaatac ttgcaggtca tttccttcaa aaaagatatt ataaatgact gatatacttt
     5401 gagttaaacc taacatacca aaaagtgcca cgtaataagt aaagaaacct ggcaatttaa
     5461 caaagttcat agcaaacata gcaaatccgt aaatcagtaa aaaaatcagc cctgaaaaaa
     5521 gattttgaga caaaagatag atagttaatc gctttccctt ttttcctttc tctcttgctt
     5581 tattcgtgag ctgtggatta acatataaaa gattaactcg aattaattct aataattgtc
     5641 gcttatacat acggtccatc tcctgtcgct tgccttccag ccattttcaa ataaatcttt
     5701 tctagcgttt catcaggtga gccattcaat aattcactaa ctgccccctg ataaatcaat
     5761 tcacctttct tcaaaatagc cagtttatcg cataactgtt gtgcggtatc caatacatgt
     5821 gtagaaaaaa tgactgtttt tccttttgcg gcatgagcct tcatcatttc tttcaaatca
     5881 aatgctgctt gaggatcaag tccttgcaaa ggttcatcca atacccaaat atctggatct
     5941 ggcagcaacg tgccgatcaa aatcgttttt tgacgcattc catgtgaaaa acttgcgatt
     6001 gtttcatttt gatgactata catatcaaaa agcgctgtca attctgctaa acgcttttct
     6061 tttttttgtg tgtctaattc ataggcagct gaaatcaagt cccaatattc tccagccgtc
     6121 aattgtaaaa aaatatctgg ggaatcaggc acataaccga ttttttgttt gatactcaat
     6181 cgattttccg tcaatttcat tccatctact aaaatcgttc ccgatgatgg ttcaataata
     6241 ctaactaaac ttttaatcgt agttgatttt ccggcaccat tatgtcctaa aaatccaaaa
     6301 atttcccctt gtcttatcga gagagacaag tcttttaatg cttgtttttt cccgtaactt
     6361 ttacttacat tgatgaattc aatcatttct gtactccttt tgtttgttat tactttttat
     6421 tgtacccttc gttattggtg gagtaaatgt caaaaagagt gcataacaaa aaaggataga
     6481 aacgactagg ctgctacttg ggttgttctc ttctacagga aaacactctt ctaacaggta
     6541 agatatgagt ctttgttttc tttaaaatga ttcattttct atcttttctt gcataaaatt
     6601 ctttagttta tcaatacttc tctttcccat ctctagttgt tctccatttg caaatgttac
     6661 tatttcgttt actctgctaa tagatgtaat gttttcagta ttaatgatat aagcttttaa
     6721 atctttacat aaatactcat attcgagtga ttttttatat tttttaattg tgccgtttac
     6781 gagaatctct tcatctttca atactaggcc tattttattt ttataacctg gcatgctcat
     6841 tagataaagg atttcatcgt aatggatata gtgatcgcta ttaccataac ggacagttgt
     6901 ccaccttttt ttcgtagcat gttgaacagg atagttttct ttttctttca ataagtaact
     6961 ttggatgctt tttttatttt tttcataagt gaattctttt atcaaataac cactaggcgt
     7021 aatctttcga ttaataacag ttattgcttt attcggatga gacgttagat aaacaatttt
     7081 acacttttca ttcttttctc taagcatttc gcctagttcg ataccgctat aataggcgtt
     7141 caaatcgatg tctaggaaaa aaatatctga ggaatagatt tcattatttt taatgaattc
     7201 aacaaattgt ataacgttca atccctgaac gatctcaaat ttccaaggac ttaataaaat
     7261 tctggattct tccaaaattt ctactagcat tttttcaaaa accaaactat catccaaaat
     7321 aaatatacgc gttaccataa aaagtacctc cactcgatat tttactatca aaatcatttc
     7381 atacaacttt tcaacataca tgttaggaat aaattgtaat tctcttcaca atcttttcca
     7441 acacctttga aaccgatatc aaatactttc aggatgctgc ttctaaaata aatatcaaac
     7501 aaatgaatac atcatgcaaa aaaagccaga caaccgacgt ccttgcgtgt cagctgtctg
     7561 agctatgttt atttattttg ttcttttaaa aagaacgata cacatactga tttttcggag
     7621 cagagacttt ctcagcgttt gtaacaacta cagatgggat ttcacgcttg aatggttgat
     7681 aatagtctga ttggatggtt ccttctattt tataccattc atcattttta accgttactc
     7741 cttccggcat atgcgtcaac aaaccaaaga ctcctgaatc tgccacacaa tggataatgc
     7801 cgaatcggaa caagaagata tctgtattgc tatcgttcgg tgtttggtaa acaaatcctt
     7861 catagctgat ttttttgcca atgaattcgc tagggtaatt ataaattagc tccataactt
     7921 ctaaatagtt ttcatctgtg atcttgatag tttgcttatc cccatacttt tctttcaact
     7981 cattcatttg gtctaagtag tctgatttgt tgaaataaat actggtatcc ggctttaagt
     8041 attgtgtctg catctccgga tcccctactg attctttact tactgggaaa ttaaatcctt
     8101 tggcttctac gatcgtagtg tcgagactaa ctgttggaaa caacgtcccc acgattactg
     8161 gcaaagctag cagtacataa gcgattccac gttgataagg ttttgacaac ccatggtcat
     8221 gatcgtgctc gtgtgccttt tcttctgctt ttccgttttt gacccacaaa tacagctgaa
     8281 ctaaagccaa aagagcagat aaaatcattg aaagaatagc taagtagcga taatggacat
     8341 tgatatattg atcgagtttg ccagaaactt gcaagtacat catcaattcg aaatacccag
     8401 ataaaatcaa aaaccgcagc atattttccc tcctaaataa ataatgtgaa tccaataacg
     8461 ctaagggcaa tcagtccgat cagcgataaa ataaagcgtc ctgtaaaata tcgtttcatc
     8521 atcagtagat ttttgatatc gaccattggg ccaaagacca aaaaggcagc tacaggtgct
     8581 gtgccgaaca gacttaacaa agaagatcct ataaacgcat ctgcttctga gcaaagagac
     8641 atcgtaaagg ccaataacag catgatgata atggctaaaa tctttgttga gccgagcgtt
     8701 agcataattc tagttggtaa ataagtctgc atcgcagctg cgattaatcc gccaatgatc
     8761 aaataacgac ctgtatcaaa aaattcgtcg attgaatgtg tcaacacatg cccagtttga
     8821 tggatcagct gattttttgg ttcattcttc tcactttgtt cttcttcatg gacatgatga
     8881 tggtgatttt ctgctacttt agacgtgatt ttttctttca atacagcgtc tttttgaaaa
     8941 taggcaagcc agacaccaat cacaagggca acaaaaaagc tgcctatcac ccgccagctt
     9001 gccatctgcc aagaattgcc aaatgcgata aaagtggaaa aaatcacgat tggattgatg
     9061 atcggagcag tcagcataaa agcaaacgct gtgtgaactg gtacgccttt tttaacaaat
     9121 tgatgaacga tcggtacgat gccgcattca catgaaggaa agaaaaatcc taagatcgaa
     9181 ccagtaaaaa tagagaagat acgattttca ggaagccatc tcttcacacg ttctggggtc
     9241 aaaaatactt gtaaggcgcc tgagataaga cagcctaata atacgaaagg taatgcttca
     9301 ataacgatcg ataagaaaat cgtactcatc tgcaaaaccg aatttggaat aaaagaaaac
     9361 atgagtttcc tcctttttat ttctagttca ttcgctttgc aaatatacgc ttttattcaa
     9421 aaaaaacaag ttgctctgta aatttttaca caactcttat tttttaccaa agcacttcaa
     9481 ttgacttttt ctgttattcc cattaaatta aggtctcata catatcactt ctatcataaa
     9541 agggagaata aaaatgaaac aaaccactaa cttgactgaa gttcaagaca tactgcaaca
     9601 gtcatccgtt gccgtcattt atctttcgat gcctaattgc agtgtttgtc acgctgttcg
     9661 tccacgacta gaggaacttc tcacacatta tgatattccg gcacttcatt tggatgcatt
     9721 tgaaacacca gaagtagcta gtaaatttga agtgctgact gcgccagttg tcctgatttt
     9781 tcatcaagga aaagaagtct ttcggcaagc acggttcatc gactttaaaa aaatccgtta
     9841 tctactagat gaactgacag cagaagatga ttccctttct tatgaagaaa tcttccgaac
     9901 aaaataatcg ttgtccaaac agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt
     9961 tgttcaatcg ctttttattt ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa
    10021 tgcaagaaat agatcaaggt ttgcaattca ttcgtcaaat caacattttg gatcaataca
    10081 tctgacggag cattcaatcg agcagcggta aagttcaaga ttcctttgat tcctgcttcc
    10141 gttaactgat tgactacttc ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg
    10201 atttgttgga tcttgatttg ttctgacata tcttccattg gataaacggg gataccgtct
    10261 acgatgcgcc caacgatttc ctcattcaca tcaaaagcac aactgatacg tatgctatta
    10321 ctttgatgga atttgtattt tagtaaagca cttcccaagt tccctacacc gatcagcgca
    10381 acatttgtca attgatcgtc gtttaatgtc tttgcaaaaa agtgcataag atcttctaca
    10441 tcataaccat acccacgttt tcctaattcg ccaaaataag aaaaatctcg acggatcgtt
    10501 gcactatcta cttgaacggc ttcacttaat tcagttgaag acactttttt ctttccagca
    10561 ttatttaaaa tccttaaata gcgataatac aaaggcagtc ttttagctgt cgcctttgga
    10621 ataatatgat ctttcacgaa atgacctcca cgtattgtct aattacatct ttgatttctc
    10681 catcatagca gtatttatag gaaaaaatca attccattgc tcagatattg tgaaatttta
    10741 ttctttattc atttatttat catttctgca aatctcgcag tataatggaa tttatggtag
    10801 actactaatt gaaaaatagt tttttatttg aggagaattc acaaagtaga aaagaggatt
    10861 ccatgatatt attacaagcc aatcagattg ctcgccattt tggtgcagaa gtcttatttg
    10921 aaaacataca tttagaaatc agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg
    10981 ctggaaaatc gactttatta aagatcattg ctggaattga agcacctgat gaaggaaaga
    11041 tcgctaaaaa taaaacagcg actctcggat acctcgctca ggacacagga cttgattcca
    11101 atgaaacagt ttggaacgag atgctgaaag cttttgaaca agttagacaa atggaagagc
    11161 ggatgcgcga gctagagatt ttcattagcg aaggtacgcc agacacacct gcttatagtt
    11221 cttatttaaa agaatacgac cggctgcaac acgatttttc agatcgaaac ggctatggtt
    11281 atgaaaatga aattcgttcc gtattgcatg ggtttaaatt cgattcttca ttttatgatc
    11341 agaaaatcga tactttatca ggcggacagc gcacacgttt agctttagct cgcatgcttt
    11401 tgcaacaccc tgacattttg attctagatg aaccaacgaa ccatttagat atcgagacac
    11461 tttcctggct tgagaattat ctgcaaagct atactggtgc attattgatt gtttcccatg
    11521 accgttattt tttagataaa gtagtcaatg aagtctatga attaagcaga agaaaaatgc
    11581 gccattacaa aggtaactat tcgaaatatc tagatttgaa agcagaacag ttagcaagtg
    11641 aatggaaggc ttatgaaaag cagcaaacag aaatccataa attagaagac ttcgttgctc
    11701 ggaaccttgt tcgcgcctct accacaaaac gagcgcaaag ccgcagaaag acattagaaa
    11761 aaatggaccg gctggatcgt cctcaagggg atgaaaaaca agcaaacttt ctattcagta
    11821 tagaaaagcc ttctggaaat gtagtcttgc aaatcgaaga tgccgctatc ggatatgata
    11881 atgtcacctt atctgaacca atcaatctag atatccgtcg tcaagaagcg attgctctag
    11941 taggtccgaa tggtatcggg aaatcgacgt tgctgaaatc aatcattcac cagatccctt
    12001 ttatccgtgg aaaagaaacg ctagggacaa atgtcaccat cggctattac gatcaaggtc
    12061 aagctgatct gcatacaaac aagacgatcc tagcagaatt gtgggatgag catcctacca
    12121 ctccagaaaa agagatccga agtgctttag gtgctttctt attttctggc gaagatgtag
    12181 aaaaaccaat ccctctgcta agtggcggtg aaaaagcacg ggttgcttta gccaagttag
    12241 cgatgaacaa agaaaatttt ttgattttgg atgaaccaac aaaccattta gatattgaca
    12301 acaaagaagt attagaaaat gcattgatcg attatgacgg cacactgctt tttgtttccc
    12361 atgaccgata tttcatcaat cggatcgcga caaaagtcgt cgaactctct gaaaatggca
    12421 gcaaagtcta tctaggagac tatgattatt atttagagaa aaagcaggaa gaagaagaaa
    12481 tcgccgaatt gttggcacaa gatgagccag aatctgctcc tgctcccacg aacaaatctg
    12541 attattacca aagcaaagaa cagcaaaagc ttatccgtag ccttcaacga aaaattactc
    12601 aggttgaaga agaaatggcg cggatcgatg aatggatcga tcagttggaa caagaaatgg
    12661 tcgcgccaga aaacctggaa gatcacgtaa aactcaatga gctgaaccaa gatttagaag
    12721 ctgctagaca a
//
LOCUS       DACCQI010000083        12680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCQI010000083 DACCQI010000000
VERSION     DACCQI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.084"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..539
                     /locus_tag="IVO76_002007"
     CDS             <1..539
                     /locus_tag="IVO76_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7429959.1"
                     /translation="YSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIA
                     HKVRVKDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTF
                     YDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNR
                     SHKGFQQVTDTLVSMFTE"
     gene            1100..1414
                     /locus_tag="IVO76_002008"
     CDS             1100..1414
                     /locus_tag="IVO76_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7429960.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            1430..1816
                     /locus_tag="IVO76_002009"
     CDS             1430..1816
                     /locus_tag="IVO76_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7429961.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1845..3230
                     /locus_tag="IVO76_002010"
     CDS             1845..3230
                     /locus_tag="IVO76_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7429962.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            3233..3385
                     /locus_tag="IVO76_002011"
     CDS             3233..3385
                     /locus_tag="IVO76_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7429963.1"
                     /translation="MLAGVAIATPALTPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            3408..4613
                     /locus_tag="IVO76_002012"
     CDS             3408..4613
                     /locus_tag="IVO76_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7429964.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            4656..4877
                     /locus_tag="IVO76_002013"
     CDS             4656..4877
                     /locus_tag="IVO76_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429965.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4994..5491
                     /locus_tag="IVO76_002014"
     CDS             4994..5491
                     /locus_tag="IVO76_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7429966.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            5466..5972
                     /locus_tag="IVO76_002015"
     CDS             5466..5972
                     /locus_tag="IVO76_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7429967.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5956..8403
                     /locus_tag="IVO76_002016"
                     /pseudo
     CDS             5956..8403
                     /locus_tag="IVO76_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            8406..10583
                     /locus_tag="IVO76_002017"
     CDS             8406..10583
                     /locus_tag="IVO76_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7429968.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGADRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRNNVNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAIIKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLSDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTALGAGSAIASATGKQGQSGSSGRTQADHTRPDGQ
                     EKSTLGKRIGQTIGAVADTKDKIVDSAGNLKEQVKDIPTNARYAVYQGKSKAKENVRD
                     LTSSISQTRADRASGRKEQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQEQS
                     HDGQTAKQSSIQTSRMESQQAKQERPAVKSDSLSLKAERQNRTTQERTVQKPATSTTP
                     ADRASQRPVTKERPSTVQRVQSQNLRNRPPIKSATIKKGSKKP"
     gene            10580..11581
                     /locus_tag="IVO76_002018"
     CDS             10580..11581
                     /locus_tag="IVO76_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7429969.1"
                     /translation="MKLKALVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            11578..12510
                     /locus_tag="IVO76_002019"
     CDS             11578..12510
                     /locus_tag="IVO76_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7429970.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
BASE COUNT         4057 a         2392 c         2658 g         3573 t
ORIGIN      
        1 tttactccag aggggttcat gatgtcttac tgtttattac cgatggctta aaaggcatga
       61 aagatacgat tcaccagatt tatcctaaag ctaaatacca acattgctgt gttcacgttt
      121 cccgtaatat tgctcataaa gtacgtgtca aagaccgaaa agaaatctgt gatgacttta
      181 aggctgttta tcaagctaac tcaaaagaag aagcgaatac cttcttatcc ggcatgattg
      241 agaaatggaa gaaaaactat cctaaagtga cgcagtcact catagaaaac caagacttat
      301 taacttttta tgattttcca cctagcattc gtagaaccat ttactcaacc aatctaatcg
      361 agtctttcaa taagcaaatt aaaagataca gccgtagaaa agagcagttt caaaatgaag
      421 aatcactaga acgctttcta gtcagcattt ttgatacata caatcaaaaa tttctaaaca
      481 gaagccataa aggttttcaa caggtaaccg atacattagt ttcaatgttt actgagtaac
      541 taattatttt gcaggaggac aatttattta cacaaaatta ttgacgctcc cataaggcta
      601 ccaacataac gatcattact tctctggtag gctatggact tctagcaatc agtattttgt
      661 ctatagatta tggagtaact atactgcaag tcttagtttt ggctatacct atgtatctat
      721 cattgtttat catgcatgaa atatataagc tatttgtttt atcaaaaata gcataaaaat
      781 ctagttatcc gcataaaaac tggacttatc acactttatc aaggtcaaaa ccactcaatt
      841 tactactaat ttactactta tgaatgagct ttgatacgac gatttatcct tgaaaagtga
      901 agatataaag atacttccaa taaaatttga atatttaata ggtagacact tcaaaaaatg
      961 aggtgtctat ttttttaccc gattttgaaa ggaagtgaac ttatgaaaac aaaaaatcaa
     1021 gaatcaaaag gtcgttcccc actctttaag accatcaaac attcattcag ccaataaaaa
     1081 agaaaggata ggtaaaaata tggaacttaa atttgtgatt cccaacatgg aaaaaacatt
     1141 cggcaattta gaatttgctg gcgaggataa agtcgttcag cgaagaatca acggacggct
     1201 aactgtctta tcaagaagct ataatctcta ttctgatgtt caaagagcag atgatattgt
     1261 ggtggtgctt cctgctgaag ctggcgaaaa acatttcggc tttgaggaac gtgtgaagtt
     1321 agtcaatcca cgtattaccg cagagggcta caaaatcggc actcgtggtt ttacaaatta
     1381 ccttttacat gctgacgaca tgataaaaga ataaagaaag agaggaaaaa tgatgagatt
     1441 agcaaatggc attgtattag ataaagacac gacttttgga gaattgaaat tctctgctct
     1501 acgtcgtgaa gtgagaatcc aaaatgaaga cgggtcggtt tcagatgaaa tcaaggaacg
     1561 tacctatgac ttaaaatcca aaggacaagg acgcatgatt caagtaagta ttcctgccag
     1621 cgtgcctttg aaagagtttg attataacgc acgggtggaa cttatcaatc ccattgcgga
     1681 caccgttgct actgccacct atcaaggagc agatgttgac tggtatatca aggcagacga
     1741 tattgtgctg acaaaggatt ctagttcatt caaagctcaa ccacaagcaa agaaagaacc
     1801 gacacaagac aaatagtcgc taggtagaaa ggagactttt tcgcatgaaa cagcgtggta
     1861 aaaggattcg cccatctggt aaagatttag tctttcattt tacgatagcg tcactcctgc
     1921 ctgttttcct gctggttgtc ggactgtttc atgtgaagac aatccagcag atcaactggc
     1981 aggattttaa cctatcacaa gcagataaga ttgacattcc ctatttaatt atcagtttca
     2041 gtgtcgcaat tcttatctgc ttgctggtag cgtttgtatt caaacgggtt cgctatgata
     2101 cggttaaaca actttaccac cgtcaaaaac tggcaaagat gatacttgaa aacaagtggt
     2161 atgaatctga acaggtcaaa acagagggtt tctttaaaga tagtgctggt cgtacaaagg
     2221 aaaagataac ctacttccct aaaatgtatt atcgacttaa aaatggcttg atacagatac
     2281 gggtggaaat cacgctggga aaatatcaag accaactctt acacttggaa aagaaattag
     2341 agagtggctt gtactgtgag ctgacggata aagagttaaa ggattcctat gtggaatata
     2401 ctttgctcta tgacaccata gccagtcgta tttctattga tgaagtagaa gctaaagatg
     2461 gtaaacttcg cttaatgaaa aacgtatggt gggaatatga taagctccct catatgttga
     2521 ttgctggtgg tacaggtggc ggtaaaactt actttatact gacactgatt gaagccttgc
     2581 ttcatacaga ttcaaaactg tatattcttg acccgaaaaa tgctgatctt gcggacttag
     2641 gttctgtgat ggcaaatgtc tactatagaa aagaagactt gctttcttgc attgaaacat
     2701 tctatgaaga aatgatgaaa cgtagtgagg aaatgaagca gatgaagaac tataagactg
     2761 gcaaaaatta tgcttactta ggtctcccgg cacacttctt aatctttgat gaatacgtcg
     2821 ctttcatgga aatgctggga acaaaagaaa acaccgcagt tatgaataag ctgaaacaga
     2881 ttgtcatgtt aggtcgtcaa gctggcttct ttctaatact ggcttgtcaa cgtccagacg
     2941 caaaatattt aggcgacgga atccgtgatc agtttaattt cagagtggct ttaggtcgta
     3001 tgtctgaaat gggctatggc atgatgtttg gcagtgacgt acaaaaggat ttcttcttaa
     3061 agcgaatcaa aggtcgtggc tatgttgatg taggaacaag tgtcatatca gagttttata
     3121 ctccccttgt accaaaagga tatgatttct tggaggaaat taaaaagtta tccaacagca
     3181 gacagtccac gcaggcgacg tgcgaagcgg aagtcgcagg tgtggactga tcttgctggc
     3241 tggtgtggca atagccacgc cagcacttac ccccccgtat ctaacagggg ggtacaaatc
     3301 gacaggaaac agtcaaaaaa acattagaaa atcctttggt tacaagggat ttacaaaatt
     3361 tcagcgtatg tcaaatgggc tttaaaagtt gacatacgcc tttttgattg gagggatttt
     3421 tactgaatga acaaacttgg ttacagcatt taaaagaaaa acgcttggct tatggactat
     3481 ctcaaaaccg tttagctgtt gcgactggta ttacaaggca gtatctaagc gatattgaaa
     3541 caggaaaagt caagccatca gaggatttac agcagtccct ttgggaagct ctggaacgct
     3601 tcaatcccga cgctcccctt gaaatgctgt ttgattatgt aagaattcgc tttccgacaa
     3661 cagacgtaca gcaggtggtc gaaaacatct tacaactgaa actgtcctat tttcttcatg
     3721 aggactatgg tttctattct tattcagagc attatgcttt aggcgacata ttcgtccttt
     3781 gctcccatga actggacaaa ggagttctgg tggaattgaa aggtcgtggg tgcagacaat
     3841 ttgaaagcta tcttctggca caacaaagaa gctggtatga gttctttatg gacgttttgg
     3901 tggctggcgg tgtgatgaaa cgccttgacc ttgccattaa cgataagaca gggattttaa
     3961 atatccctgt actcactgaa aagtgccaac aggaagaatg tatctccgtc ttccgcagtt
     4021 ttaaaagcta tcgcagtggc gaactggtac gcaaagagga aaaggaatgt atgggaaaca
     4081 ccctctatat cggttcatta caaagtgaag tttatttctg tatctatgaa aaggactacg
     4141 agcagtacaa gaaaaatgat attcccattg aagacgcaga agtaaaaaac cgttttgaga
     4201 ttcgattgaa aaatgagcgt gcctattatg cagtccgtga tttactcgtc tatgacaatc
     4261 cagagcatac cgcctttaaa attatcaatc ggtatatccg ttttgtagat aaagacgatt
     4321 ccaaacctcg ttctgattgg aaactgaatg aagaatgggc ttggtttatt gggaacaatc
     4381 gtgaacgatt aaaactaacc acaaaaccag agccttactc cttccaaagg acgctgaact
     4441 ggctatctca tcaagttgcc ccgaccttaa aggttgcgat taaacttgat gaaatcaacc
     4501 agacgcaggt tgtaaaagac attctcgacc atgcgaaact gacagaccga cacaagcaga
     4561 ttttgaagca acagtcagta aaagaacagg acgtgataac aacaaaaaaa taactcaaat
     4621 acaaattcat tgaatataga gaggagaaca tttttatgaa ttttggacaa aacctttata
     4681 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
     4741 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
     4801 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
     4861 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
     4921 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
     4981 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
     5041 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
     5101 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
     5161 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
     5221 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
     5281 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
     5341 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
     5401 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
     5461 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
     5521 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
     5581 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
     5641 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     5701 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     5761 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     5821 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     5881 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     5941 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     6001 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     6061 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     6121 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     6181 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     6241 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     6301 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     6361 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     6421 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     6481 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     6541 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     6601 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     6661 gaagaaaaac agtgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     6721 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     6781 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     6841 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     6901 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     6961 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     7021 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     7081 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     7141 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     7201 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     7261 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     7321 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     7381 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     7441 gtgattgtag acccaaaagc agaacgtggc agatggaaag aaaccttgcc agagatttcc
     7501 catgaaatca atatcgtcac tctgacttct gatgagaaaa acaaaggctt acttgaccct
     7561 tatgtgatta tgaaaaatcc caaagattct gaatcactgg ctattgatat tctgacattc
     7621 cttacgggga tttcctctcg tgatggggaa cgcttcccaa tccttagaaa agccattcgt
     7681 gcagtaacca atagtgaagt acgagggttg atgaaagtga ttgaggaatt acgggttgag
     7741 aatacgccac taagtaccag tatagccgac catatcgaaa gttttacaga ctatgacttt
     7801 gcacatttat tattcagtaa tggttatgtg gagcagtcta tcagcttaga aaaacaactg
     7861 aacattatac aggttgcgga cttggtactt cccgacaagg aaacttcctt tgaggaatat
     7921 accactatgg agcttttatc cgttgctatg ctgattgtca ttagtacctt tgctttagac
     7981 tttatccata cagaccgaag cattttcaag attgtagatt tagacgaagc atggagcttt
     8041 ttacaggtag cacaaggaaa aacactatct atgaagctgg ttcgggctgg tcgtgctatg
     8101 aacgctgggg tatatttcgt gacccaaaat acagacgacc tcttagatga aaaactgaaa
     8161 aataacctcg gcttaaaatt tgcatttcgt tccactgacc ttaacgagat taaaaagacc
     8221 ttagcctttt ttggtgtaga cccagaggac gaaaacaatc agaagcgatt gcgtgatttg
     8281 gaaaacgggc aatgccttat cagtgattta tatggtcgtg tcggtgtgat acagttccac
     8341 cctgtatttg aagaactgct ccatgccttt gataccagac cacctgtgcg aaaagaggtg
     8401 taaatgtgaa accatcaata gtaaacagaa taaaatcaaa ctggacgctg aaacgtctag
     8461 gtaaagtggc aatgacagtg gctttcacac ttgtgattgc catttttctt ttagccatgc
     8521 tgggaacggt ggttcaagct gcgggcttgg tagatgatac ggtcaatgtg gcaaatgaat
     8581 acagccgata cccacttgaa aactatcaac tggattttta tgtggataat agctggggct
     8641 ggcttccgtg gaactggtcg gacgggattg gaaaacaggt catgtatgga ctatatgcca
     8701 ttaccaattt tatttggaca atcagtttgt atgtttccaa tgcgacaggt tacttagtac
     8761 aggaagccta ttccttagac ttcatttccg ctacagcaga ttccattggt aagaatatgc
     8821 agaccttagc tggtgtgagt gcaaacggat tttcaacaga gggtttctat gttggattcc
     8881 tcttactctt gattttggtt cttggggttt atgttgccta tacgggactg ataaagagag
     8941 aaaccacaaa ggcaattcat gccattatga attttgtgct ggtgtttatc ctatcggctt
     9001 cctttattgc ctacgctccc gactacatta aaaaaatcaa tgacttttca tcagacatca
     9061 gtaatgccag tttatcactt ggcacgaaga ttgtcatgcc ccattccgat agtcaaggca
     9121 aggacagcgt ggacttaatc agagatagcc tgttttccat acaggttcag caaccgtggc
     9181 tactgcttca atacaacagt tcagacattg aaagtattgg tgctgaccgt gtggaaagtc
     9241 tactttcaac cagtccagac agtaataatg gagaagacag agaaaaaatt gtagcagaag
     9301 aaatcgaaga cagaaacaat gtgaacatga cgattacaaa aactattaac cgtttgggta
     9361 cagtcttctt tctctttgta ttcaatatcg ggatttctat attcgtattc ctactaacag
     9421 gaatcatgat tttctcgcag gtacttttta tcatctatgc catgtttctg cctgtgagct
     9481 ttattttaag catgattccg tcctttgatg gtatgtcaaa acgagccatt atcaaactct
     9541 ttaataccat tttgacacga gctggaatca cgctgattat tacgacagcc tttagtattt
     9601 caaccatgct ctatacctta tcggctggtt atccgttctt tttgattgcc tttttacaga
     9661 ttgtgacctt tgcaggaatc tacttcaaac tgagcgattt aatgagtatg ttttcgcttc
     9721 aaagcaacga ttctcaaagt gtgggaagtc gtgtgatgag aaaacctcgt atgcttatgc
     9781 acgctcatat gcaccgccta cagcgtaaac ttggacgctc catgacagct ttaggtgctg
     9841 gctctgccat tgccagtgct acaggaaaac aaggacagtc gggtagttct ggtaggacac
     9901 aagcagatca cacccgtcca gacggacagg aaaaatcaac acttggaaaa cgtatcggac
     9961 aaacaatcgg agcagttgct gataccaaag ataaaatcgt tgattctgct ggtaatctca
    10021 aagaacaggt taaggatatt ccgaccaatg caagatatgc agtgtatcaa ggaaaatcca
    10081 aagcaaaaga aaatgttcgt gacctaactt ccagtatttc tcaaaccaga gcagacagag
    10141 ccagcggacg caaggaacag caggaacaaa gacgcaaaac cattgcagag cgtcgctctg
    10201 aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc cgttcatgaa agaccagcta
    10261 ctaagcaaga acaatctcat gatggacaga ccgcaaaaca atcttctatt cagacttcac
    10321 gcatggaatc tcaacaagcc aaacaggagc gtccagcagt taagtccgat tctttaagtt
    10381 taaaagcgga acgacaaaac cgtactacac aagaaagaac agttcaaaag ccagcaactt
    10441 caactacacc agcagacaga gcttcacaac gtccagtcac aaaagagcgt ccgtctactg
    10501 ttcaaagagt tcaaagccaa aaccttagaa atagaccacc aataaaatcc gccactatca
    10561 agaaaggcag taagaaacca tgaaactgaa agctttagtg attggtggtt ctgggctatt
    10621 cttgatggtc ttctcactgc ttctgtttgt tgccatttta ttttcagatg aacaggacag
    10681 cggaatttcc aatattcatt atggaggtgt gaatgtttcc gcagaagtgc tggctcataa
    10741 gcctatggta gaaaaatatg ccaaagaata tggcgttgaa gaatatgtca acatacttct
    10801 tgcgattata caggtggaat cgggcggtac tgcggaagat gttatgcagt cctcggaatc
    10861 cctcggtctt ccacctaatt cattgagtac agaagaatcc attaagcaag gtgtgaagta
    10921 tttcagtgaa ttattagcca gtagcgaaag gctcagtgta gatttagaat cggttatcca
    10981 gtcctacaat tatggtggtg gtttcttagg gtatgtggct aatcgtggaa ataaatatac
    11041 ctttgaactg gctcaaagtt tctcaaaaga gtattcaggt ggcgaaaaag tgtcttaccc
    11101 caatcccata gccataccta tcaatggggg ctggcgatac aactatggca atatgtttta
    11161 tgtgcaactg gtaacgcagt atcttgtcac aacagagttt gatgatgata cggtacaagc
    11221 catcatggac gaagcactga aatatgaggg ctggcgatac gtttacggtg gagcttcccc
    11281 gactacttct tttgattgta gcggactgac acaatggacg tatggaaaag ctggaattaa
    11341 cttaccacga accgcacaac agcaatatga tgtgacccag catatcccac tatcggaagc
    11401 acaagctggc gatttggttt tctttcattc tacctataac gctggctctt atattactca
    11461 tgttgggata taccttggca ataaccgtat gtttcatgca ggcgacccaa tcggttatgc
    11521 cgacttaaca agcccctact ggcaacagca tttagtggga gcaggacgaa tcaaacaatg
    11581 agaaaggaag atttaatgat gaaatttaga aaaaatcaga ataaagaaaa acagatacca
    11641 aaggaaaaga aacctcgtgt ctataaggtc aatcctcata aaaaggttgt gattgccttg
    11701 tgggtacttt tagggcttag tttcagcttt gcgatattca agcactttac agctatagat
    11761 actcatacta ttcacgaaac aactatcata gaaaaggaat acgttgatac tcatcatgta
    11821 gaaaattttg tagagaactt tgcgaaagtc tactattcat gggagcaatc cgataagtcc
    11881 attgataatc gaatggaaag tctaaaaggc tatctgacag atgaacttca agctctcaat
    11941 gttgatacag tacgcaaaga tattcctgta tcgtcttctg taagaggatt tcagatatgg
    12001 acggtagagc caactggcga caatgagttt aatgtaacct acagtgtaga ccagctcatt
    12061 acagagggag aaaatacaaa gaccgtccac tctgcttata tagtgagtgt ctatgtagat
    12121 ggttctggaa atatggtact ggttaagaat ccgaccatta ccaacatacc taagaaatca
    12181 agttataaac caaaagccat tgaaagtgag gggacggttg attccattac aaccaatgaa
    12241 atcaatgagt ttttaacgac gttcttcaag ctctatccta cagcgacagc cagtgaactt
    12301 tcctactatg tgaatgacgg gatattaaaa ccaatcggaa aagagtacat ctttcaagaa
    12361 ctggtaaatc ctattcacaa tcgtaaggat aatcaagtca cggtatcgct gacagtggag
    12421 tatatcgacc agcagaccaa agcaacgcag gtatctcaat ttgatttggt acttgaaaag
    12481 aacgggagta attggaagat tgtaaaataa caaatattgg tacatgatta cagatacttt
    12541 gtaatcatgt actctttttg ataaaaaatt ggagattcct ttacaaatat gctcttacgt
    12601 gctattattt aagtgactat ttaaaaggag ttaataaata tgcggcaagg tattcttaaa
    12661 taaactgtca atttgatagc
//
LOCUS       DACCQI010000084        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCQI010000084 DACCQI010000000
VERSION     DACCQI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.085"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IVO76_002020"
     CDS             6..305
                     /locus_tag="IVO76_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7429971.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVO76_002021"
     CDS             378..1739
                     /locus_tag="IVO76_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7429972.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVO76_002022"
     CDS             1761..2021
                     /locus_tag="IVO76_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429973.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVO76_002023"
     CDS             2035..2787
                     /locus_tag="IVO76_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7429974.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVO76_002024"
     CDS             complement(2881..3486)
                     /locus_tag="IVO76_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7429975.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVO76_002025"
     CDS             complement(3539..4513)
                     /locus_tag="IVO76_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7429976.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVO76_002026"
     CDS             complement(4891..5670)
                     /locus_tag="IVO76_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7429977.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVO76_002027"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVO76_002027"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7429978.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVO76_002028"
     CDS             7161..8558
                     /locus_tag="IVO76_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7429979.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVO76_002029"
     CDS             8639..9079
                     /locus_tag="IVO76_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7429980.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVO76_002030"
     CDS             9227..9553
                     /locus_tag="IVO76_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7429981.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVO76_002031"
     CDS             9554..10303
                     /locus_tag="IVO76_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429982.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVO76_002032"
     CDS             complement(10383..10838)
                     /locus_tag="IVO76_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7429983.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IVO76_002033"
     CDS             complement(11265..11540)
                     /locus_tag="IVO76_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7429984.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IVO76_002034"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IVO76_002034"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7429985.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2175 c         2587 g         3852 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACCQI010000085        12382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCQI010000085 DACCQI010000000
VERSION     DACCQI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.086"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            3..1214
                     /locus_tag="IVO76_002035"
     CDS             3..1214
                     /locus_tag="IVO76_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7429986.1"
                     /translation="MYQKGYNMLEELRHEADAPVVKAKIFNKEAITIYGSSAAKVFYD
                     PRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDLMTPERMEDYHRILDKN
                     LTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEVEKLAEYQISMISGTFT
                     SPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAFANATDLDGQLLPLEVA
                     AVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTLQDPFIQEMRRYYPFFP
                     MLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDSFMIKRYVGKAKDISYK
                     EEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEFSVPEQDWTIPFNQFPT
                     YPNSRALLYKN"
     gene            complement(1303..2250)
                     /locus_tag="IVO76_002036"
     CDS             complement(1303..2250)
                     /locus_tag="IVO76_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7429987.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2644..3936
                     /locus_tag="IVO76_002037"
     CDS             2644..3936
                     /locus_tag="IVO76_002037"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7429988.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            3951..5720
                     /gene="aspS"
                     /locus_tag="IVO76_002038"
     CDS             3951..5720
                     /gene="aspS"
                     /locus_tag="IVO76_002038"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7429989.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5829..6704
                     /locus_tag="IVO76_002039"
     CDS             5829..6704
                     /locus_tag="IVO76_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7429990.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            6970..8853
                     /locus_tag="IVO76_002040"
     CDS             6970..8853
                     /locus_tag="IVO76_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7429991.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(8928..9212)
                     /locus_tag="IVO76_002041"
     CDS             complement(8928..9212)
                     /locus_tag="IVO76_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7429992.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9202..9936)
                     /locus_tag="IVO76_002042"
     CDS             complement(9202..9936)
                     /locus_tag="IVO76_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7429993.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10065..10688
                     /locus_tag="IVO76_002043"
     CDS             10065..10688
                     /locus_tag="IVO76_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7429994.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            10880..12001
                     /locus_tag="IVO76_002044"
     CDS             10880..12001
                     /locus_tag="IVO76_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7429995.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12008..>12382
                     /locus_tag="IVO76_002045"
     CDS             12008..>12382
                     /locus_tag="IVO76_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7429996.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKE"
BASE COUNT         3919 a         2211 c         2671 g         3581 t
ORIGIN      
        1 aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg gacgctcccg
       61 tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca tcagcagcaa
      121 aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa ttagtattga
      181 agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat caccatcgga
      241 aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat cgtatcttag
      301 ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta tttgatttaa
      361 gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta tcagcgattt
      421 ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc ggaacgttta
      481 cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa aaatgggcgc
      541 aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat gtggcattat
      601 atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa gtggcagctg
      661 ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg gcattgatgg
      721 gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac ttttcgacgt
      781 tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca atgctacctg
      841 cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc agttgggtta
      901 ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca gattccttta
      961 tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa tatgagatga
     1021 ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa tggatcactc
     1081 ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc tctgtacctg
     1141 aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc cgagcgctac
     1201 tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa cagtgctgat
     1261 cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc cacgaaatgc
     1321 ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt ggacattgtc
     1381 agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg gattcttcat
     1441 gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa tggggcgaag
     1501 tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg gatttctttt
     1561 tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc ctgtcagcaa
     1621 accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa gataagggaa
     1681 taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta catcgacata
     1741 tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac cgactgctcg
     1801 gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt ttggtgtgct
     1861 ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac gttttagtgc
     1921 atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc ctttatttgg
     1981 tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt tttccttcag
     2041 tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg tatcgatcaa
     2101 attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat cttccaaacc
     2161 gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt ctgcgtagac
     2221 ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt ctagtctaaa
     2281 acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc agatatctct
     2341 tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat gaaagagcga
     2401 gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc taaagtttca
     2461 ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta cgagcgttat
     2521 tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa tttcattcac
     2581 ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa gggagagaaa
     2641 actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga atcagaaaaa
     2701 tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta taatgaaatg
     2761 cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga tacaacggat
     2821 atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt gacgcttcga
     2881 ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt cggcccagag
     2941 tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga acgccctcaa
     3001 aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc agaaaaccct
     3061 gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt ggggatcacg
     3121 caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc ctatcgtcaa
     3181 gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc taaacgccgc
     3241 cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca aaaatttgtg
     3301 gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca ttttgatacc
     3361 gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa tatggttcgc
     3421 ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc aaaaatgggg
     3481 gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga actaggtggt
     3541 ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt gactatggag
     3601 gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt cgcactcgga
     3661 gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt tggtttttca
     3721 gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc agataaagcg
     3781 aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac gattaatgtc
     3841 aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata tgaacatttt
     3901 gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa atggcacaaa
     3961 gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt acattaaaag
     4021 gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta cgtgatcgtg
     4081 aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg gaaatcgcgg
     4141 ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga cgcggagaaa
     4201 atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt gaaatcgtga
     4261 ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca atcagtgacg
     4321 aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact aaaaacatca
     4381 aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat gactttctcg
     4441 atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac tacctagttc
     4501 cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa ttgtttaaac
     4561 aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt ttccgcgatg
     4621 aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa acaacgttct
     4681 taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta atgaaagaag
     4741 ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa gcaatggctc
     4801 gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat ctaagcgata
     4861 cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc ggtgtcgtga
     4921 aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg gatcagttag
     4981 gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta gaagaagacg
     5041 gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc attaaagcaa
     5101 cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa atcgtcgctg
     5161 ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt gatgaatcga
     5221 aattcaattt cttatgggta actgattggc cacaatttga attttcagaa gaagaaggtc
     5281 gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc ccgttattgg
     5341 ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac ggttatgaac
     5401 taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta ctagaaactc
     5461 taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca gctttagatt
     5521 acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca atgttattag
     5581 ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa gcagcagatg
     5641 tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg aatattgatg
     5701 taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa tacgcttctt
     5761 ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat ataaagcggg
     5821 gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg accaagcttt
     5881 cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt tatcagccag
     5941 gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact ttgtcaaaat
     6001 cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat atacctttgt
     6061 ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt atcacagtca
     6121 ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc gatccaatca
     6181 tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg ttgagaaacg
     6241 gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa acaggaaagt
     6301 cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc ggaattctat
     6361 ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga aaagtaactg
     6421 atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa catccagaaa
     6481 aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc aatgaagcag
     6541 aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca cgatacgaac
     6601 tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta agtattgcag
     6661 aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag tttgacaaaa
     6721 attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt ctaccatagt
     6781 agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg aaaaaaagca
     6841 tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag gacacgattc
     6901 tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta tgaaaagttg
     6961 gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac tgggtttgat
     7021 gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc aactagtatg
     7081 aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt gatcacggat
     7141 ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt ttatgtcaga
     7201 agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc ggcttatatc
     7261 actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag cagcttactg
     7321 tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg atttgctaat
     7381 gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta tttggtttgg
     7441 ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat cttttatact
     7501 gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac accaggaggc
     7561 gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg gttgggaagt
     7621 gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa acaagcgaaa
     7681 aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg cttcttcttt
     7741 ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag tctgttatgc
     7801 gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg gctgacaaat
     7861 ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca gtttccagtt
     7921 atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt gcatacttta
     7981 gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt ggagcgtccg
     8041 ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt cagcaaaccg
     8101 atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc aattgcactc
     8161 gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca actgcaggca
     8221 aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga aaacaccact
     8281 agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca agggaataag
     8341 gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc tgcaacggat
     8401 ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca attatatgcc
     8461 agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac tgttctgatc
     8521 ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat ggatgcaatc
     8581 ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg gcaaaatgac
     8641 gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct gattgattgg
     8701 tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca tccaaatcct
     8761 gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg aagttcaagt
     8821 acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc aggcaaaaac
     8881 atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca tatagattca
     8941 ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg ggtgagtttt
     9001 ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc tgcatggatc
     9061 atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc attgttatgt
     9121 tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg atctttacag
     9181 aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct ctgcagtgta
     9241 ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt ctaatttgcc
     9301 ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg ggtaaacaaa
     9361 ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt ctaagaaccg
     9421 atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac ttgaaatatc
     9481 gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt gtttattagg
     9541 gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga ctaagtcaca
     9601 agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga cagtcatttg
     9661 ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac gaggttgaag
     9721 ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa ctgcgccatt
     9781 tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt gagcaagcag
     9841 cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga tatcttctgc
     9901 gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa atgaaaaaaa
     9961 tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt caaacctcta
    10021 ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt tatcgattta
    10081 tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc tatgagaata
    10141 aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt acttggtggg
    10201 acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg gcaaaagaag
    10261 aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct tttccagtta
    10321 aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg aaatcgacaa
    10381 atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt aaaggcggaa
    10441 tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat caaggtccgt
    10501 tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc gaaccaatcg
    10561 acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa cgacgaacac
    10621 aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat gaagtcaaaa
    10681 aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc ctgttcagtt
    10741 cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt tgcgttccaa
    10801 tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata attagaagga
    10861 cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata tcggaaaaaa
    10921 actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta tactagatat
    10981 cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg ataggagtgt
    11041 cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga atttaaagga
    11101 aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac tttcagcggg
    11161 tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag aagcatttga
    11221 accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac caattgcagc
    11281 gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag ccaaggtgat
    11341 agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta gccatttttt
    11401 tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg ggttggattc
    11461 gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt tgcataatca
    11521 agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat tctctttatc
    11581 tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc tggagttgaa
    11641 ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt tcaaagaatt
    11701 gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata tctatctcac
    11761 tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc ctcgtatttt
    11821 ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga
    11881 aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg aagagcagct
    11941 aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg
    12001 atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta tcaatgagac
    12061 cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga agacaggagc
    12121 tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa cttccggaag
    12181 acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt
    12241 gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac cagaacaagt
    12301 tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa cgttgaccat
    12361 ttttgatggg aaaggaaaag aa
//
LOCUS       DACCQI010000086        12207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCQI010000086 DACCQI010000000
VERSION     DACCQI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.089"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IVO76_002046"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="IVO76_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7429997.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="IVO76_002047"
     CDS             complement(2064..3062)
                     /locus_tag="IVO76_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7429998.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="IVO76_002048"
     CDS             complement(3288..3941)
                     /locus_tag="IVO76_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7429999.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IVO76_002049"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="IVO76_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7430000.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            5436..6143
                     /locus_tag="IVO76_002050"
     CDS             5436..6143
                     /locus_tag="IVO76_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7430001.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            complement(6289..9483)
                     /locus_tag="IVO76_002051"
     CDS             complement(6289..9483)
                     /locus_tag="IVO76_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7430002.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(9502..>12207)
                     /locus_tag="IVO76_002052"
     CDS             complement(9502..>12207)
                     /locus_tag="IVO76_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7430003.1"
                     /translation="LMNLDAKQFSQIVLLPQGEFRNFLVSSSSDKETVLRHLFGTQFF
                     QQFNERLKEKAKQQQQKLDHLEQELHLLQKRFVLVKEEEPAAGFEAVLAQWTNHQAIL
                     EKQITEEKEALTELQEKQKQLENSYYRFESLKKSYEEKTILINKQKELAAQKDEIEEK
                     KRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQKSSEQTKKLAMTTQNYEEWQKNEANR
                     VRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQDAEQLTISLDKEELNAEKLYQNLAIY
                     QERTRILQENLLDQEKLQEDRLAFGQLKYLDEKQQEIEKQKAKDTGSLAQIREELKKA
                     TEEITKNSQLLKEQTEQAEQLKSQWAKQQIARLQLFLRPGEPCLVCGSTEHPIQQLKH
                     EQPNKEDILKVEQQLAEAESAVERTQQIIAKSEAHENNLQQQLSDLEQKIMATNIQLA
                     EQEEHLQQALKKAFPEITEQKTKTTLVVIEQSLDRRKSKMANARIELEEIRQQAEKLE
                     QQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQDTDSNCLEEEIRRLEKQLEEDSNQLA
                     VEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEKIEKEQERIAKKLESQSYFSTIEEIC
                     AFAQQQAQYKENQRTVTSYHEEQLVTADRLRQLENIQLTETFPDTEEISQQLEEIRRM
                     IEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQTKLDELTQLQQLSQTFNGENPKKTS
                     LERYVLQVYLQEVLQVANDHLQRLTKNRYQFELADTIGSYRGKTGLEINIYDDQAGMS
                     RSAHTLSGGESFIAALSLALALADVIQTQAGGVAIDALFIDEGFGSLDEEALEMAMEA
                     LETIESEGRMIGIISHVRELKERVLQQIRIDTEGSGQSKIRYQLG"
BASE COUNT         3368 a         2494 c         2010 g         4335 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttttaaaaa
     4801 aatgaaagtt atctaaggtt aatatacagt aaaaccagca ttctcgcttt ttttatcatt
     4861 taaaaattag agttttcaaa tctaattcaa attttctaaa catatgaaac agcgtataaa
     4921 actgtactat tattttatct caaaagctaa atgaaaccga tttcttaaga ttttttatgt
     4981 tttttatttc tttaatagta atgttttctg tcattttaaa taatactgtt ctttattata
     5041 acgcttattt tctctgttat ataagcgtag tattttaaag caatgattac taaataaaat
     5101 ttactataaa caaataaatc aaataacgtt attttatgtt tgaatagtta tattgagact
     5161 gtattaaaaa aataaatctt tcgaaaattc cacagagatt gcattaaaat aaatcaatcg
     5221 acttctacca taatgattaa tttgttcatc aaacttattg acgtgtttca cgatttttag
     5281 tgaaaaaaac tgaaatataa aaaccttatt ctgtcaagga aatagaaata tagtaagaaa
     5341 aacctttatt tatcagcttt tttagacaat tttttctgtt acaatagtca aggatttgtg
     5401 aaaaaatcaa tgaaaaccga aaggcgtgta aaattttgat taaatcagtt gtgtttgatg
     5461 ttgatgatac gatgtatgat cagcagcagc catttcgaaa cgcggtcaaa agagtcgttc
     5521 cgcttgtttc agatgcagat atgcatccac tatatatccg gttccgccat catagtgatg
     5581 aaaacttccc aaaagtaatg gcaggagatt ggacactaga atatatgcga gcacatcgga
     5641 tcagccaatc actaaaagat ttagactacc ctcatattac ggaagaagat ggcctcttat
     5701 ttcaaaaaat ttacgaggaa gaacttgata atatctgctt gcatgaggag gtcaaaaaaa
     5761 ccctcgattt cttgaaggaa aagaatgtcc cattaggaat tatcacaaat gggccgactg
     5821 accatcagac aaaaaaactg aagcaacttc aattgaacaa ttggattcct tcccgcaata
     5881 tgctcatttc tcaagcaaca ggtttccaaa aaccagaaaa agagattttt cagctagctg
     5941 agaaagaatt tcatatgctt ccagaagaga ccttatacgt tggcgataat tacgacaatg
     6001 atgttctagg tgcaaaaagt gctaactggc aagcgctttg gttcaaccat cgagaacgca
     6061 aaattgaaca gactccaatt tgcgatatcg agatttcttc ttttgatcag ctgttagaga
     6121 caatgaagac aatctttgta taacctttaa aaatagaaat aaaaaaatga tagcaaggaa
     6181 gctgggataa aatggaatcc cagcctcctt gctatttttt aaatcaaata aacaatcgct
     6241 tctttagaaa ggctttctta cccattactg aacgcttttt cgagtctctt actcctcgat
     6301 ttctcctaca cttaaaatca tgcggatgtc ttcttcagtc agtttcgtca actgttcttc
     6361 attaccttgg atcacttttt ggaacagttc gcgtttttct tgctggagtg aatccatgcg
     6421 ttcctcaatc gttccttcag agatcatacg ccacacttct accacatttt tttgtccgat
     6481 ccgatgtgca cgtccagcag cttgttcttc aatggcagga ttccaccaaa ggtcatacaa
     6541 gatgaccgta tctgctcccg tgagattcaa tccagttccc ccagccttta aggaaatcag
     6601 aaaaacatct ttttcgccag cattaaatgc gtctaccata cttaaccgat cttgtggttt
     6661 cgtactgcct cttaaataaa atgtcgagat ccctagctcg gctaactctt cttgcaagat
     6721 agtcagcatc cctgtaaatt gagaaaacag caaaactctt cgtttgttct ctttggcagc
     6781 taccagtaga tcttttactt gttcgagctt gccggaaccg ccttggtaat cttcaataaa
     6841 cagacgagga tcgcagcaaa tttgtcgcag tcttgtcaga ccagcaagta tcccgatccg
     6901 gtttttcttg aatgcttcag aatccattga tgtgatttct tcacgcattt gacgcaagta
     6961 agctaaataa acagtttttt gttcttctgt aagtgcagaa tatagattca tctctgtttt
     7021 ttccggcaag tctttcaaca cagtttgttt atctcgccgt aaaataaacg gtcgaatcat
     7081 ttgcgcaatt tcttctgcgg caagttccct gaattttgtt ttagtcggga aaaatcctgg
     7141 cataatcgtt gcaaacaatg accacaattc ttctaaatta ttctcgattg gtgtcccact
     7201 cagtgcaaac cgctgaggta cagctaattc tctcaatgct tgtgcagttt tcgtactgct
     7261 atttttgacc atttgtgcct catcaagaat caaataatca attttttgcg actgataatt
     7321 agctagatcc tgtcttaagc tggcataaga agtcactcgg atatctgtgt ctttagccag
     7381 taattcttca cgttcttttt tattcccatt gattacttgg atacttaaag aaggggcgaa
     7441 ttttctcacc tctgcctgcc agttgtaaat taagctcgca ggcgctacga tcaaagcaga
     7501 gaagcttttc cgttcttctt tttccgaaag taaaaaggca attgtctgta gtgttttccc
     7561 aagtcccatt tcatcggcta aaataccgcc aaactgataa tgacctaaca tcttcaacca
     7621 tcgaaaacct tgcttttgat aatctctcat cgttgcattc aatcctttgg gaagctgtgc
     7681 ctggtatctt tctggatgaa tgagatcttg taccatcgtt tggaacgatt cactaaaagt
     7741 agcattgctt ttttccaatt gattttgaat gatcaatcct tgatttttag gtacatggat
     7801 cgttccttct tctgtacgta tcgattcccg taattgttgc aacacttggc ttgtttgttg
     7861 aaattcttct gaatcaaaag aaagaacctg accattttct aatgtataaa aagcatcatt
     7921 tctcagcaag ctttgcagga catgatcgat ttcttgctcc tctatccctg tcacatcgaa
     7981 tcgaatatcg agccatgaac cggtctcggt tacttcgatt ttcggttgat gtttatgcgc
     8041 atctaaatac aactctcgca attttcttcc aagtctgact tcaccaaact gacggaagac
     8101 agatagttct gtgcggaaaa aggcatatag ttcttccccg cttggcaatg gacgttcata
     8161 accagtttca tttttctggt agcgatacat tctgaataga tcaagtactc gtttctcttc
     8221 tactgtgtca cgtaacacct gaaaagcctg ttctgtcgag acctcatgct tcgggtcagt
     8281 cgaatagatg acttcgccat agacgaaatc gactcgagct tggatttttc cttttgcttt
     8341 tcggaaaaag aaaataaatt cgacggggat atctgctact tcctcttcta ctgcaggggc
     8401 aatagctacg tcgccgatct ctgacagcag agggataatc tctttaaaca aagacggtaa
     8461 ttcttttttc ttgaacacta cttctggttt ctccaatcgg cgaatcaatt gtttccaagt
     8521 ttgataaacc gtttgttgat atttcgtcag agaataaaat gtcccttgga taactcccca
     8581 ctgataatgc ataaagaaaa aatcaaaatc attcaatgct tgcagcaaat aatcttctcc
     8641 aaatttttcc actttgaatg ataaaggttt gttttgatcg gaaaattgta ttccttgatc
     8701 gttttgttca tttatctgca gatgcaaacg tgtagtctga ttcatctgct ctaacaggaa
     8761 gcgggcttgt tctactggga gaagcagata tcttttatct agttttcctt tgacttgtaa
     8821 gccgttcgta cctaataatt gatgcgtctg agcacttcct gcaagatgcg tgagtatttt
     8881 ttgatcttct ttttgaaacg cccgtttatc tagacgaaac tggtactgtt tattaacagt
     8941 gtaagtttct ttttctgtgt aagcttgtaa aaaatcatag atgtttttta cgatatatgt
     9001 ttttttcggt gatgccaatg aaccgatacg caagtaaagt gccaaaatag aaagctctgg
     9061 gtgatactga ttagtctcaa tcgtttctac ttggtattca ataatcaaag gttgcaccgc
     9121 ttgaggattg ttttgtgttt ttagacgaga aaaacctttt gcaatgattt ctccctcact
     9181 tgttgctttt accggttcta gcattggctg gtcttttttc atgatacgag atagattttt
     9241 cgaccgtaaa taaagttcca cagcaactgt atgcttacaa tatttatgtt cctcccaata
     9301 gggacattgg cagtaatcta tctcttttgc tgtaccatcc aaatcaacca tatacaattc
     9361 actgcccaag acttctgcat gccacacgtt ttcttcttgg tcagggacaa ccgataacac
     9421 tcggccttct tgcaagtatt gacgtccacg tgcaatgact ttttctggaa tactccattt
     9481 catagttacc aatctccctt tttatccaag ttgatagcgt attttgcttt gtccgctacc
     9541 ttctgtatca attcttattt gttgtaatac tcgttctttc aattctctta catgactaat
     9601 gatgccgatc attcttcctt cactttcgat cgtttctaaa gcttccattg ccatttccaa
     9661 agcttcttca tctaatgacc caaatccttc atcaataaac aatgcatcga ttgctacacc
     9721 accagcttgt gtctggatca catcggcaag agctaatgct aaagaaagtg ctgcaataaa
     9781 actctcaccg ccagacaggg tatgcgcact tcgagacata cctgcctgat catcatagat
     9841 attgatttct aatccggttt tccctcgata actgccaatg gtatccgcta gctcaaattg
     9901 ataacgattt ttcgttaaac gttggagatg atcatttgct acttgtaaca cttcttgaag
     9961 ataaacctga agcacatacc gttccaaact agtcttctta ggattttctc cgttgaatgt
    10021 ttgcgaaagc tgctgtaatt gcgtaagctc atctaatttc gtctggcttg tgtgataaat
    10081 cgaaagacat tcatcgtaga ttttttgatt actaattttt tgttcttgta aaccgtatat
    10141 cttttcttgc tttatttcga tcatacgtct tatttcttct aactgttggc tgatctcttc
    10201 tgtatctggg aatgtctcag ttagctggat attttctaat tgacgcagcc ggtcagctgt
    10261 cactaattgc tcttcatgat aagaagtaac tgtacgttgg ttttctttat attgtgcttg
    10321 ctgttgtgca aatgcacaga tttcttcaat tgttgaaaaa tacgactggc tttctaactt
    10381 tttagctatt ctttcttgtt ccttttctat tttttcatct attttttcta tttgttcatg
    10441 ttgaagatca agctgtgtcc gaagcgtatg aagatcttgc aacaattgca ctcctgcttt
    10501 ctgctcaaca gctagttgat tcgagtcttc ttctaactgt ttttcaagtc gtcgtatctc
    10561 ctcttctaaa cagttactat ccgtatcttg caattgttgt tctaaacttt tgatttctcc
    10621 ttgcagttgc tggagctgat tatttttttc tgtctgctgc tgtttgtaga cagctaattg
    10681 ttgttcgagc ttttctgctt gttgtcttat ttcttccagc tctattcttg cattggccat
    10741 cttgcttttt cgtctatcta aagattgttc gattactaca agagttgttt ttgttttttg
    10801 ttctgttatc tcaggaaatg cctttttcaa agcctgttgc aagtgttctt cttgttcagc
    10861 caattgtata ttcgttgcca tgattttttg ttctaaatct gataattgtt gttgaagatt
    10921 attttcatgt gcttcacttt tggcaatgat ttgctgagtt ctttctacag cagactcagc
    10981 ttctgccagt tgttgttcta cttttaagat atcttcctta tttggctgtt cgtgttttaa
    11041 ttgttgtatg gggtgttccg ttgatccaca aacaagacat ggctcccctg gacgcaaaaa
    11101 aagctgaaga cgagcaattt gctgctttgc ccattggctt ttcagctgtt ctgcttgttc
    11161 tgtctgttct ttcaatagct gactattttt tgttatttct tcagttgctt ttttgagttc
    11221 ttctcttatc tgtgcgagcg acccagtgtc ttttgctttt tgtttttcta tttcttgctg
    11281 cttttcatct agatatttta actgtccaaa agcaagacga tcttcttgca gtttttcctg
    11341 atccagtaaa ttttcttgaa gaattcgagt tctttcctga tagatagcga gattttgata
    11401 cagcttctcg gcattcaatt cttctttatc caatgaaatt gttaattgtt ctgcgtcttg
    11461 ttttttacta cgtaaggatt cgacaattgg caccatcatc tgtttgtttt gcagtttttg
    11521 agcagcttct ttgatttttg tttgtcggcg tacgcggttt gcttcatttt tttgccactc
    11581 ttcataattc tgcgtagtca tcgctagttt ctttgtttgc tcactacttt tttgtgacag
    11641 ttctattttt tcaactttta tttcttgtag gcgctggatt ggttcgacta atcgttgcgt
    11701 aaattcataa tacccaatcc atcgtttctt ttcctctatt tcgtcttttt gagcagctaa
    11761 ttccttttgc ttgtttatta atatcgtttt ttcttcataa ctttttttca agctttcaaa
    11821 acgatagtag gaattttcta gttgcttttg tttttcctga agttccgtta gagcttcttt
    11881 ttcttccgtg atttgttttt cgagtattgc ctgatggttt gtccattggg ctaaaacggc
    11941 ttcaaaccct gctgctggtt cctcttcttt gactaaaaca aacctttttt gcagaagatg
    12001 cagctcttgc tctaaatgat ccaatttttg ttgctgttgt tttgcttttt cttttaatcg
    12061 ctcattgaac tgttggaaaa attgagtacc gaataaatga cgtaaaacag tctctttatc
    12121 actgcttgat gaaaccaaaa aattacggaa ttctccttgg ggtagtaaaa cgatttgaga
    12181 aaactgtttc gcatcgagat tcatcag
//
LOCUS       DACCQI010000087        12109 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCQI010000087 DACCQI010000000
VERSION     DACCQI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.090"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            213..1691
                     /locus_tag="IVO76_002053"
     CDS             213..1691
                     /locus_tag="IVO76_002053"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7430004.1"
                     /translation="MKLKENWSRTDRYRPYKEWSEEHLHSLEKKINSSHWRLGYHIQP
                     VTGLLNDPNGFSYFDGKWQLFYQAYPMGSVHGLKSWYHLSSVNLIDWKNDGLKLIPDN
                     SFDSHGVYSGSALPIDNQLFLAYTGNVRDSNWGRHSFQLGAWMDKNGNITKIPTPLIS
                     SPPPGYTQEIRDPQLIAYDDGYLLIVGAQTEQKQGRILTYYSKDLQNWQFLGTLGFTE
                     EEMGFMIECPNLLFVNGKALLVFCPQGLSHEIHSYSNIYPNTYVIGQTFDFEKNELSD
                     PTHIKNIDEGFDIYATHCFSDENGRTLAISWIGLPEISYPTDEEGWAHCLSIVKELTI
                     EKEQLYQRPVKETAQLRKGKPYHFSGLETTIDAEINQYELQLEFDYSQKGTILLFADE
                     QQKHGLALSFDMIHGKMSMDRSHAGKSFAENFGTVREFSIEKKPLKLQIFVDRSVVEV
                     FVNDGQQTATARVFPTDDQTGLYIKSDREFKGKLWHLRNMNK"
     gene            1704..2687
                     /locus_tag="IVO76_002054"
     CDS             1704..2687
                     /locus_tag="IVO76_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7430005.1"
                     /translation="MAVKLTDVAKKAGVSPTTVSRVINNYGSLSQKTIDKVNQAMKEL
                     NYQPNSLARSLQGKNTQLIGLIFPTVANPFFGELVEQLESKLFDLGYRSILCDSANNK
                     EKERNYLNMLAANKVDGIIAGAHNLGIQEYENIELPIVSFDRYLADGTPIVSSDNFRG
                     GYLATENLYLKGARKIAILTGSEESNSPTNDRMNGYLKFMDEQQLEPQIFQFHSTARS
                     TALKNLEIKRILQNEEIDSIFCTDDLTAVLAYNLCQELDIRIPEQLKIIGYDGTKFIQ
                     NYFPHLSTIAQPITDIADILVDLLLQRIQDAEKPLESHYVLPVKLIQGKTT"
     gene            2709..3590
                     /locus_tag="IVO76_002055"
     CDS             2709..3590
                     /locus_tag="IVO76_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7430006.1"
                     /translation="MLYGAIEAGGTKFVCAVGTKDLEVIERIQIPTTTPIETMNNVID
                     FFKKHQEQLAAIGIGSFGPIDIHPNSATYGYITSTPKKAWQNFDFIGYLNKNLKELPI
                     VFTTDVNAAAYGEYVAGNGKGRSTIVYYTIGTGIGGGALQNGTFVEGFSHPEMGHMLV
                     RRHPVDTFKGVCPYHSDCLEGMASGPAIEQRTGMKGQELSVADPSWELEAYYIAQCVY
                     NTTLLFSPDVIIFGGGVMKQAHLRERVQEALVGLMNHYVDYPAFENYVLPPKLGDDAG
                     IIGCLALALEKSEKDIF"
     gene            complement(4342..4905)
                     /locus_tag="IVO76_002056"
     CDS             complement(4342..4905)
                     /locus_tag="IVO76_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7430007.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(4920..5963)
                     /locus_tag="IVO76_002057"
     CDS             complement(4920..5963)
                     /locus_tag="IVO76_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430008.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDKKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMQTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(6096..6500)
                     /locus_tag="IVO76_002058"
     CDS             complement(6096..6500)
                     /locus_tag="IVO76_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7430009.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(6668..7303)
                     /locus_tag="IVO76_002059"
     CDS             complement(6668..7303)
                     /locus_tag="IVO76_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7430010.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLGSMIFGVLTRLE"
     gene            complement(7569..8621)
                     /locus_tag="IVO76_002060"
     CDS             complement(7569..8621)
                     /locus_tag="IVO76_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7430011.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(8634..9572)
                     /locus_tag="IVO76_002061"
     CDS             complement(8634..9572)
                     /locus_tag="IVO76_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7430012.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(10023..10673)
                     /locus_tag="IVO76_002062"
     CDS             complement(10023..10673)
                     /locus_tag="IVO76_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430013.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(10773..11282)
                     /locus_tag="IVO76_002063"
                     /pseudo
     CDS             complement(10773..11282)
                     /locus_tag="IVO76_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
     gene            complement(11645..12028)
                     /locus_tag="IVO76_002064"
     CDS             complement(11645..12028)
                     /locus_tag="IVO76_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7430014.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNKVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
BASE COUNT         3816 a         2426 c         2016 g         3851 t
ORIGIN      
        1 ccaaaattat tgaagcagta actataaatt tttcttaagc acaatatgcc atgatttaaa
       61 acaattgtca atcgattatc aaattttaaa taaaataatt ttttatatat aaaaaaatag
      121 cttttataag ccatttaatt ttgatattcg tttgacattt taccagaaaa aatatattat
      181 aattccaaaa atacgtttac aaggagtttg tcatgaaact taaagaaaat tggtcacgaa
      241 cggatcgtta tcgtccatat aaagaatggt ctgaagaaca tttgcattcc ttagagaaaa
      301 aaatcaattc ttcccattgg cgcttaggct accatataca accagttact ggtttattga
      361 acgatccgaa tggtttttcc tatttcgacg ggaaatggca gctattttat caagcatatc
      421 cgatggggtc tgttcatggt ctgaaatctt ggtaccatct ctcctcagtt aatctgatag
      481 attggaaaaa cgatggcctc aaactgattc cggataattc atttgacagt cacggtgtgt
      541 actcaggttc agctttgccg attgataatc aactgttcct tgcatatacc ggaaatgtgc
      601 gtgattcgaa ttggggacgt cattccttcc agttaggggc atggatggac aaaaatggta
      661 atatcactaa gataccgact cctttgatca gctccccacc tccgggatat acacaagaaa
      721 tccgtgatcc tcagctgatt gcttatgatg acggttactt gctcattgtc ggagcacaaa
      781 ctgaacaaaa acaaggaaga atcctaactt attacagtaa agatcttcaa aattggcagt
      841 ttttaggaac tttgggtttt acagaggaag aaatgggttt tatgatcgag tgtccaaatc
      901 tattatttgt gaacggcaaa gcactactgg tcttctgccc acaaggttta tctcacgaga
      961 tccactcata cagtaatatt tacccgaata cttacgtgat cggtcaaaca tttgattttg
     1021 aaaaaaatga gctttccgac cctactcaca taaaaaatat agatgaagga tttgacattt
     1081 acgcgacgca ttgtttcagt gatgaaaatg gacgaacctt ggcaatcagt tggattgggt
     1141 tgcctgaaat cagctatcca actgacgaag aaggatgggc acactgcttg agcatcgtaa
     1201 aagaacttac tattgaaaaa gagcagctct atcaacgacc ggtgaaagaa acagcgcagt
     1261 tgcgaaaagg aaaaccttat cactttagcg gtttggaaac cactattgac gcggaaataa
     1321 atcaatatga actccagtta gaattcgatt actcacagaa aggtactatt ttattgtttg
     1381 cagatgaaca acaaaaacac ggtttagcac tatcctttga tatgatacat ggtaaaatga
     1441 gtatggatcg cagccatgcc ggcaaatctt tcgctgaaaa ttttggaact gtacgtgaat
     1501 tctcaattga aaagaaaccg ctgaaattac aaatatttgt tgatcgttcc gttgtagagg
     1561 tgtttgtaaa tgatggacag caaacagcga ctgcccgtgt ctttccgaca gatgatcaaa
     1621 cgggcttgta tataaaaagt gacagggaat ttaaagggaa attatggcat ctgcggaata
     1681 tgaacaaata aaggatgaag aaaatggctg ttaaattgac agatgtggca aaaaaagcag
     1741 gagtctctcc tactactgtc tcgagagtga taaataatta tggttcttta agtcaaaaaa
     1801 caatcgataa agtgaatcaa gcaatgaaag aactcaatta tcagccgaat tcgttagccc
     1861 gttcacttca aggaaaaaac acccaattga tcggcttgat tttcccaaca gtcgccaatc
     1921 cgttttttgg cgaattagtc gaacagttgg aaagtaaatt atttgattta ggctatcgtt
     1981 ctatcttatg tgacagtgct aataataaag aaaaagaacg aaattatctc aacatgttag
     2041 cagcaaataa agtggacggc atcatcgccg gcgctcataa tctcgggata caggagtacg
     2101 aaaatattga gttgccaatc gtctcttttg accgctatct ggcagacgga acaccaatcg
     2161 tcagcagtga taatttccga ggcggttatc tcgcgacaga aaatctctat ctaaaaggag
     2221 ctcgtaaaat cgctattttg acgggctcgg aagaatccaa ctcccccaca aatgaccgaa
     2281 tgaacggcta tttgaaattt atggatgagc agcagctaga accacagatt tttcaattcc
     2341 attcaactgc tcgttccact gctttaaaaa atttagaaat caaacggatt ttgcaaaatg
     2401 aagagattga cagtattttt tgcacagatg atcttacagc tgtattggca tacaatctgt
     2461 gtcaagagct cgatatccgc atccctgagc aattgaaaat cattggatat gacggaacaa
     2521 aatttatcca gaattatttt ccgcatctgt ctactatcgc tcaaccaata acggatattg
     2581 ccgacatcct tgtagacttg ctactgcaaa gaatccaaga tgctgaaaaa ccactggaat
     2641 cacattacgt tttaccagta aagctgattc aaggtaaaac gacttaactg caaaggagca
     2701 aatgttttat gttatatgga gcaattgaag ctggcggaac aaaatttgtc tgcgcagtag
     2761 gtacgaaaga tttagaagta attgaaagaa tacaaatccc tacaaccacg ccgatagaaa
     2821 cgatgaataa cgttattgat ttcttcaaaa aacaccaaga acaactggca gcgattggaa
     2881 taggttcttt tggaccgatt gatattcatc ccaattcagc aacatacgga tatatcacct
     2941 ctacgccaaa aaaagcttgg cagaattttg attttatcgg ttatttaaat aaaaatttaa
     3001 aagaactacc gattgtcttt accaccgatg tcaatgcagc tgcttacgga gaatatgtcg
     3061 cggggaatgg aaaagggcgt tccactattg tttactacac gattggaaca ggtattggtg
     3121 gcggtgcctt gcaaaatggc acttttgtag aaggtttcag ccaccctgaa atgggacata
     3181 tgcttgtgcg ccgacaccct gtggatacct ttaaaggtgt ttgtccttat catagcgatt
     3241 gcctggaagg aatggcttct ggacctgcaa tcgaacaaag aacaggcatg aaaggtcaag
     3301 agttgtctgt cgctgatcct tcttgggaat tagaagctta ttacatcgct caatgtgtct
     3361 ataacacaac tcttctattt tcacctgatg tcatcatctt tggcggaggc gtaatgaaac
     3421 aagcacatct gagagaacga gtccaagaag ctttggttgg tttgatgaat cactatgtag
     3481 actacccagc attcgaaaac tatgtattac ctccaaaatt aggagatgat gctggaatca
     3541 ttgggtgttt agctttagca ttagaaaaat cagaaaaaga tatcttctaa taaaataagc
     3601 acgttgtcac aacaatctca tcaataagca ggcgaagaat ccacttgatg cgatttcttc
     3661 gtctttttat ttactattaa cttatgttct ccaatcggcc tttcaacccg atatcctcgc
     3721 ctatttttct tgcccttaac tctctgtttc ctgtacttaa cagagtagag gtgtcgtgga
     3781 attcttttgt taacttcaaa ttatgatctt ctacaagttc ctacaatcta atcttggttg
     3841 tttttttatt caacttgcaa cgctagcaag ttccagttga ttctcttttc caattaagtt
     3901 ttgtgatatt taaacttcgc cttgacagct tttctaaata tagaaaagaa catttcctta
     3961 tgtctattat gcaacagctc aatttattta atgtatgtta atgattttgt ggaatgtctc
     4021 cttagacatg ctatttctac tatttaatga tcaatcactt gacctactag taagtagaga
     4081 gcatgcccga acacattaca aaaatatcat cacttatgat acggagctgc gtatcatgag
     4141 tgaatgatat taggaaaact tcataaaata aatcttttat acatttccac ttctagatgg
     4201 tcattcatgt tagaagaatc aaactatttt tttactgtcg taataatatt aaaacactag
     4261 aacaaatcaa ttaaattcca taaaatataa ataatagacc tagcatcatc tggtataaaa
     4321 tggatgctag gtccgttata tttactcaaa attgattcta cgaatagatc ttcttatcat
     4381 ttctatttta gtagtcatgc gttgttgttc ttctaaaggt ggtaatggta taagtagttt
     4441 agataaattt gtaccattta catttggttg cccagttccg taagaaattt tttctaataa
     4501 cttccaatat aatggcgagt ctaagaagca gtctacgtat tctgataata atttctcaac
     4561 taacctaatt cgaatcaagt aagaagcaaa aacagattcc tcacttattt ccttaaccaa
     4621 gaaacttttt cccatagttc ctcctgtcct tgctatcaaa atgtcatttt cttccaatct
     4681 taaatcaact agtttagaat tactaatatc acagtaaggc acactggacc aatttacacg
     4741 tccctcttgt atatctgtga ttctcaaaaa cttaacattg ccttgttttt tcgccgaatc
     4801 tgtatagcca tattgaatct tagtagaaat acttcctaaa taaacccata gtcgaacatc
     4861 gctagatgat ttttcttgtc gttctttatg aacaacagtg atcatcttga gttgtccgac
     4921 tattttttag ttgcagcaat ctttttggga cgcttatttg catgaagcaa cgttgctaag
     4981 tctagtaata aaacattggc taacacagga ttggctttct cgcgatacgc tgcggcgcca
     5041 ctttcaaaca atactgctag acgatcttct acaacaacga tttgttccaa atacggtgga
     5101 aatttgattc gttttaggat ccggctagga gagtcccgct cggacactaa gagttccgag
     5161 gtataaggac cataagaacg actggctaag agataatggc cataaaaagc cacaccttga
     5221 atcttatcag atgtttttct gctcattgtc acggttggtt ggattttgtc tttgcggcga
     5281 attgctgttt gcatttcagc tatcgtcggc aatagataga aagtcaactg tccttcacct
     5341 tttagtgtga agttgccaga taccagttga tcattgtttt gagtcaaata cgatgcttgt
     5401 ggaatctctg agagatcagt tgtcagccaa taagcgattg gctgtcccgt ctcttcagat
     5461 gtatctgcta agatatcgat caaacgtaaa gcggctactc gtccagtagc agatcctgtg
     5521 attgtcaacc ataaaagttc ccgtttttta tcatatacta agccccctac atgtggccgt
     5581 ttaggtaaaa tcagcagttt gatcggctgt cctgtttctc tatccagtac atgaataata
     5641 gaatgatgct gatggtcata gcagtaggca gaaataaaca caaaatcttg agaaaaagcc
     5701 aacccttgcg gcgtcatcca ttgacaaacc tcattttcgc cggtttccgg caccaccatt
     5761 cttgtttctg ttaatccagg aatgttatat tgttttgctt cagccttttc ccaagctgct
     5821 tgaacaatag gataccgttt taactttttt tcaatatcct gatgtatccg ttgctgcttc
     5881 caatgcttaa actttaggac atagtaaaca aggaggccaa tgaccagcaa cactaaaatc
     5941 attattgcta cccgctgtgt catttttttg cctccttgtt ctattcgatc aatcgtacat
     6001 ataccgctat taattaccgt aactacatgt ttcttttact agcgtttctt tataggatct
     6061 ttcataatct catttatagt aacattaaga atcccttatt ctactatagt cacttccaca
     6121 tcaatattgc ctcttgttgc tttagagtat gggcaaaatg cgtgggcttc ttctgcgatt
     6181 ttctctgctt ggtccttctc aagtcctggc aaagaaactt ctaaaacaac cccgatttgg
     6241 tagcccccat cttctaattg aaataatgat acccgtgctt tgacttcaga atcagactca
     6301 agattatttt cttccagcat gtgttgaacc gcgccgttga agcaagatgc gtatccagcc
     6361 gcaaaaagtt gttccggatt tgtcccttca ctatggattc ctgggcggtc gatcttcatg
     6421 tgcatacttc cgtttggtgc ctcaacctcg ccatcacggc ctccacgatt gatcattgct
     6481 gtttcgtaca tttttttcat tgagatcatc ctttctttcg tcgttctata ttaagtgtaa
     6541 cgagtatttt ttaatctgac aaaggtaatg cttgcattcg gcgacatcag ttagacaaca
     6601 tcgatgatca gtgatagaag cagtttatct atacaaaaaa agaagggttc tatgatattt
     6661 ggtgttgtta ctcaaggcga gtaaggacac caaatatcat agaacccaaa tagttaaaga
     6721 aaaaacttaa tcgttgaaag aagcccaatt gcttaaagaa ggcaaactga ctaatcgacg
     6781 gaatccgtgc taatccgtaa agacctgcaa ttagtaaact aataactgtt aaaacaagat
     6841 aaaaatggct aagcgtacct tgaccacttt gacgataaag cagaacaagt aacaatggga
     6901 aaagtagaaa gcctgcatag cctagcccag aacctatatt atggatccaa gtcgatagat
     6961 tccaagagct ttcattcgta ttgatactga ataacccagt aaaaatacaa tcacctatcc
     7021 catataaacc aactgttgca acagcaagta ttgcaagtgt ttttgatgtt ccaacaaata
     7081 ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc agatacaact gaccagacca
     7141 agaaagcacg tctgactgga ctatctacgt caccaaacac actaattacg gtagtcattt
     7201 gattcagttt gggataaaaa aggccaagga tatacggtgt cgataaatca ctaatcacac
     7261 ctaataataa aaaataaaaa ccatattttt gcaaaaaaat cattttcttc ccctactttt
     7321 tagtgattat tcgatacatc caacaatcaa attttttcac attttaacac atatttttat
     7381 tctagttgcc ttttgaacaa tcaataacat aaaggtgagt ttcttctaca ttttaaaaaa
     7441 tcgaatggca ctgaagagtt cagttgtcag catcaatctc ctactcaaaa aggcaaaaaa
     7501 acgtaagtca gatgttgttg acatctcact tacgcatcca ttgattgaaa atttcttagt
     7561 aaaatctact agcctaaaaa tttgaagact tcttctcgat catacagttt aaatactttc
     7621 tcgcataaaa attgagtaat tagtgccgca aaaacaggga tgactaacca acaaagaaca
     7681 acaagaaagc catttagccc tgcatctaaa gaagccaacg gaccaacaag tccgaccacg
     7741 ccgaaaccag cagaagcagg cgtacctgaa atagaaaata gagcgacagg aatagctgta
     7801 atcactgcac taactaaaca aggaataaga atcacaggtt ttctaaatag attaggcatc
     7861 atcatcttca tagcgcctaa agcaatcgca attgtcacac ctgacttatt aaccttccaa
     7921 gagttcacta ctaaaacaaa agtggttgca gctacgccca tcgcagccgc accagcagaa
     7981 acaccatcta gttggattgc cattccgatt gctactgttg aaataggaga gataatcaaa
     8041 aaggcaaagg aacaaccaat caagatagac attaacagcg gttgtaaggt agtgaagtta
     8101 ttaatcaatg atccaattgc agaagtaatt gctgttacaa aagggaataa ataaaaacca
     8161 agtaaacctg cacctacacc tacaacaatc ggtgttaaca caatcgacac tgacccaaaa
     8221 ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg ctgtcagcat cgtattaata
     8281 atatcgcctg taccagcacc aacgtaaaca cccgtttcac ccaaatcagg tgtgaatttg
     8341 actacaccag aaccaacaaa agaagctccg gctacaacca tcattctcat cggatcaaat
     8401 ccaaattgta aggcaatcaa accaccaatg atcaaaggtg ttgctagttg aaaaataact
     8461 gctaactgtg cgatcgttac cgcaaaggga tacgcactaa aatattttaa aatggcacct
     8521 aatactgcat taggaatcaa tccaataatg attcctgttg ctgtaccagc gaggacttta
     8581 ttcagaaaaa ttttcggtga tattttttgt gaagtttcca ttcaatctct cctttatttt
     8641 acgttcaaac tatcttcttt tgcatggaca agctcagtca agaaatcaca ataaggcgtc
     8701 gcaacaccat atttcttccc ttttctagaa attgcgccat tgatataatc gatttctgtt
     8761 aatcgatggt tcttaatcaa gtcttgatac attgaaggat aatgtaaccc aattgtttcc
     8821 gggtcaaaac aagattcaca atgtgcaatg acttctggga catctagttc aatcttctct
     8881 actgctgcta ctttggcaaa ctcattgaca atcgtcgcca ccattttatg agcagtcgat
     8941 gtttttccta gctcagccat attaacgtct aagattgtgc atagtccgtt cattgttccg
     9001 ttcacacatg ctttgcgata aatagaatag tgaatattgt cagaaaaatg agcatttaat
     9061 cctgattcag acaatttgtc tgctaatttt tttgcagctg cttcttttcc atcacctaaa
     9121 ttttgtaatt ctactgaacc acttccaaat aatttgacct gaccaggacc ttcaagacct
     9181 gccgtccaca tggtatttcc aatatagata ttttccattg gtacaaattt ctcaataata
     9241 tcttcatgcc cgataccatt taatagacat aagacctctg tatctttttt gattaatgat
     9301 tggatatcct gcagcatttt ttccagctgc atcgctttgg taaatagaat aatcagatca
     9361 acttgatctt ctttttctac ttcggattga agaacgattg gtagttttgc ttctacctct
     9421 ttaccattaa aatttgcttg taatccatgt tctttgattt gttggacatg ttctgcccag
     9481 ccatcgatta ataatacttc gttcccactt tggtgaagca ttaacccaaa gcgacttccc
     9541 attgcgccag cgcctgcaat tgctattttc attgattctt cctccttttt ctttctatta
     9601 tacttatttg agaatcattt cacaaccttg ttttgttatt cttctcttat ttatcatgtt
     9661 tatatccacc attcaagtta ttttatcagc aaatcaataa aaactggaat agaatcccaa
     9721 tttcatcgta agagaaacta gactagcaat aagaaaatag aaacattcat agaaaaaacc
     9781 tcactcctct gattataaaa gaggggacat taacctcgtg tatactaaaa ccttccattc
     9841 attcgatgga tgattttctt tttgtgaaaa aatattcatt gtagtacgat gttgcgcaac
     9901 acagacggat ggtattgctt attatttatt aaaatcgaac aatacactta tttaaaaact
     9961 tctttcccag attttcttcc atttttacag ttataactag tattatcatg aaaaatagcc
    10021 ttttattgtc tatgggaata ataatttaaa attggtactt ttttattaat tagttcatcg
    10081 cccaataatc tctccagttt cttagcatct cctgactcaa ccggaattat ccaagcccag
    10141 tccaaataat tttttaaaat ctcatcatat tttgcaaata tttcattgcc atattccgtc
    10201 cttaaaatta tattttcagt gccagacaag tgatttgtat tgagttgata aaaaagatct
    10261 ccatctaaaa aaatatcatt agaagaagat ttccccacag taatgaccat tccatcagct
    10321 ctatgtgtta catacaaata attgtatgac ctcacttcac ctaatgaatc aattatttct
    10381 tgccctttag ctctaatata ttgatctcta ttacctatct cactcttatt taacaaaagt
    10441 tttttcatgt caaatttaat cggctgtttt atttttgtaa attcgcttct actactaatc
    10501 caatccgaga ttaatttttc aacatgtccc agagtaaact ctgatttatt cgctaaatac
    10561 tttttttgtt gtattaaaga catctttccc cgaagctcaa aattgagcct acatcgattc
    10621 tctatctctt caaaaagcat cttatcttca tcattcttat attcatcatt cataaaacca
    10681 atccagctcc tcgattaaga taatatttta atctcataca aaaactagct ctcatcaaaa
    10741 caaccattgt tttgatttct catgaaattt ctttatactt ttctccgatt ataaattcct
    10801 aaccccttga gaaaattacg catatattta tctccgcaag gcttaaaatt tcgttggcct
    10861 tcttttctca aaaatgcccc gatttcgcct tttgatggat agatttctgc ctcagcaaaa
    10921 acgtctaaaa tatcatcagt ggtcatagac aaggcgattt ttaatttttt tagcaacaca
    10981 ttgttgatat atttgatttc cttgcttttt tacggcgtgt ctactatcgg cttatctttt
    11041 tgcggaccac gtgccaagag aatcaggcca tttaggaatg cgtcaaaaac ttggttgttg
    11101 atggtttttt catacacatt attttctgca ttatctttgt tcttatcctg tactttggtc
    11161 agtaccgcct gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa agctttaatc
    11221 aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca ataacctatc attgttattc
    11281 atttgttcct ccgaagtatt tagtctttac tttcttacgt actttttgtc ttgctaagat
    11341 ttttaggatc aatgataaaa accgcatcac ttatgatacg gagctgggta tcatgagtga
    11401 atgatattta taaatcactg gatattgaag tatattctgt tttctcaact tatttagtca
    11461 gattttttaa tgaagatttt ttgagtaaaa tttctataat tcctacttat tgaaaagtag
    11521 tttgtagtgc tgcttctacc tctctcatct gttctacaaa ttccaaataa cttgacgcag
    11581 cttgcggaat gaaaaaatga tatgtgaata tttctcctcc tagcccgctt aatatgccta
    11641 ttttttatac ccatgatttg caggaagggg atcggaagta ggttgcagaa agccgccttt
    11701 cataatctgt cggaaaccgg acatcaaaac ggattatttt acttgtcaca tttgtatttt
    11761 cttgtgagga agtgccaaat accttattta cttgagtgac attctcattg tcctgtctga
    11821 tattgcttcg tataaaagta ccagatccac gtctcgtata taacaaacct tcatcaataa
    11881 ggagttgtat agctttgcgg atagtaacac ggcttgtttt aaactctcta gccagacttt
    11941 cttgctcagg taatttagaa ccttcctgat attttccctc aacgatcctc tcttttagag
    12001 ccaaggctat ttctttatat tttggcatat tttcccctca tttcattgat cctacaaaaa
    12061 aattagtata tatacaaatt tttgtaaagt atatatacta ataaataca
//
LOCUS       DACCQI010000088        12091 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCQI010000088 DACCQI010000000
VERSION     DACCQI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12091
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.091"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(107..760)
                     /locus_tag="IVO76_002065"
     CDS             complement(107..760)
                     /locus_tag="IVO76_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7430015.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1048..1568
                     /locus_tag="IVO76_002066"
                     /pseudo
     CDS             1048..1568
                     /locus_tag="IVO76_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1470..2186)
                     /locus_tag="IVO76_002067"
     CDS             complement(1470..2186)
                     /locus_tag="IVO76_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP7430016.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2236..2445)
                     /locus_tag="IVO76_002068"
     CDS             complement(2236..2445)
                     /locus_tag="IVO76_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430017.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            2722..3995
                     /locus_tag="IVO76_002069"
                     /pseudo
     CDS             2722..3995
                     /locus_tag="IVO76_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4102..5133)
                     /locus_tag="IVO76_002070"
     CDS             complement(4102..5133)
                     /locus_tag="IVO76_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7430018.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5140..5970)
                     /locus_tag="IVO76_002071"
     CDS             complement(5140..5970)
                     /locus_tag="IVO76_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7430019.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5967..6773)
                     /locus_tag="IVO76_002072"
     CDS             complement(5967..6773)
                     /locus_tag="IVO76_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7430020.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6779..7603)
                     /locus_tag="IVO76_002073"
     CDS             complement(6779..7603)
                     /locus_tag="IVO76_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP7430021.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7593..8693)
                     /locus_tag="IVO76_002074"
     CDS             complement(7593..8693)
                     /locus_tag="IVO76_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7430022.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8871..9656
                     /locus_tag="IVO76_002075"
     CDS             8871..9656
                     /locus_tag="IVO76_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP7430023.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9689..10072
                     /locus_tag="IVO76_002076"
     CDS             9689..10072
                     /locus_tag="IVO76_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7430024.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10148..10279
                     /locus_tag="IVO76_002077"
     CDS             10148..10279
                     /locus_tag="IVO76_002077"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7430025.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10248..10484
                     /locus_tag="IVO76_002078"
     CDS             10248..10484
                     /locus_tag="IVO76_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7430026.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(10562..11533)
                     /locus_tag="IVO76_002079"
     CDS             complement(10562..11533)
                     /locus_tag="IVO76_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP7430027.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3635 a         2738 c         2216 g         3502 t
ORIGIN      
        1 tatgttttag gaaagataaa aaaaattaat gaatgaacta atataattat tattccaaga
       61 acagaaaata tttgtatgaa catttttgga aatatcttta attttttcat tttctctcca
      121 atttatatcc tacatttctt atagtagtta tacagtctaa ttgtaatttt tttctaagtt
      181 cttttatata tacatctatt actcgatcat aaggagtttc ttcgctatct ttccatacat
      241 aatcaataat ttgcattctt gttaacactt gtccattatt atccaataaa cattttaata
      301 cttctagttc ttttgcattt acatctattt cttcaccatt tattttagct gtatagctat
      361 taaaattaac cgatagatcc ttatattcaa acttctctaa atgtccataa ttcttcttaa
      421 ttaaagaatc tattctagct tttaagaccg gcaatgaaaa tggcttttct acatatccgt
      481 ctactaaatt agtaaatgca tcaattttat attcttcatc actaaatgca gtcaagatta
      541 gaattggtaa attgcttttc tttctaattt ctttcaacac ttctaaacca tttataaaag
      601 gtatctgaat gtctaagata accaaattta tatcgctatt aaattttgac aaggcttctc
      661 ttccatcttt agcttgaata acagtatatc caaattctga aagatattca cttattccct
      721 ctcttatcat attatcatct tcaacagtta atattttcat atatacctcc acttaagcct
      781 atacactaga tattatacag catattatgg ctctataata tctagtgttg gtgacgcctt
      841 ctgttgccaa tactttttct tttttttaat aaatgcaaac agacaccttg aaatccttta
      901 gaaaaaaggc ttctataata ttttgtgttg ttggaaaatc gaaggcgatt ttctactaac
      961 actttttctt cgtttcaact caaaaagaga ctcacttttg gtaaaattta gtcgaccaaa
     1021 acatcaccct accaaaggag tcgctctatg gataatcata ctagaaaatt actcggatta
     1081 acagataaac atttattttt tgatgaagaa tggctgattg aaaaagaata taaaggcgtt
     1141 caagcgcagt ttattaaagg gagattggat gactcacctt cccattgtga acactgtgga
     1201 tcgattcgta ttatccgcaa cggttcctat acgacacgta ctcagatgtt gaaagtcaaa
     1261 gaaaagttga ctattttaga attaaaacgt acccgttttc tatgctatga ttgtgggcag
     1321 accttttctg cgaaaacgga cttagttgat gaacatcatc aactaacaaa ggaactgaag
     1381 caggcgattc tcatagaact gtacgaaaat caatcacgta atctgaaaat cagaagaagt
     1441 accgtcggct caagcgatat tggaaactct tactcaaaga ttccacgaca ttggagccgc
     1501 tcaaacggca ttatcatcga ctgtttaaac gaccaattag tcaaacagaa atcgtcgatg
     1561 aaccgttgtg cacaatggtg tcctgaacgt acagcgacac cttccatatc cagagcagtc
     1621 gccacatcac ggggatggat gccatccaaa ttaaaagcaa ccacgccagt atgatgttca
     1681 ggatcttgcg gtccgtaaat ggtgataccg ccgatcgctt gtaatttagg taaaacgtag
     1741 gcaaccagtt cttgctcata acgatgaata ttctccatac caatctcatt caagtaatcg
     1801 attgctcctg caatgttcgg agtaccagct tcaaatttcc atggcagctc tttccatgaa
     1861 ctatcgtaaa gattgacaaa atctatcatt tcaccgccga attcgactgg ctccatttgt
     1921 tctaacaatt gacgtttgcc atataataca ccgattcctg tcggcccgca cattttatgc
     1981 ccgctaaagg cgaaaaaatc tgcatccaac gcttgtacat caactgtcat atgcggtgct
     2041 gcttgggcac cgactggact atcctcaact acaacagcct gttcaaccct cagccgtttc
     2101 aagcacaaca aataaccttc tggatctggt ttgccgacct gaagatcatc gatcgtcaca
     2161 aaggaatcaa ttttttcctt aatcatcgga tgtgcttcaa aaaaatagtc tagctcccgc
     2221 agttcagaat tgagactact ccgattttcc agtgctgctg atccgctgct caaaatgttc
     2281 agcatccact ggtctcccaa ccgcttgact tatcccttca taaaaggaca gttctgctgc
     2341 cgtcccaaca ccttgttgat agtctgcaaa aggaatttta tagccgaact gctccagtaa
     2401 ataggcttca gtaaatagtg atgaatactc tcgtaaaaca tccattcact gtcaatcatc
     2461 gtcccatcaa aatcaaaaat aagtgctttg gtcatcttct ttccccctta cccaaaacaa
     2521 gcaaaagctg tgtcctgctc tctctacagt atcagcataa aaactgccgt ctgaaaagtc
     2581 aaaggtgaaa gaaatgtggc gctataattt ctaggactga tagtaaatca ttctatcagt
     2641 ccttttgagc ataaaaaaac aaaacataca gaagctccgt tagaattgaa tcaccacaac
     2701 acaatcaaag gagcgttctg tatgcctgat cttcatctta tcaaactatt actaggaatc
     2761 aaagatggaa acattcaaat cactcatgtc gagaagaaaa aaatacattc aatcaacagt
     2821 atcgttgttt ctgccacgct cattaaaacg attcagccct gtcctcagtg taaacaggtg
     2881 aatcaacatg ggctgatcgt taagaacgga aagaaaaaat cgctggtcct tctcaataag
     2941 tgtgcgaatc aacggactta tcttgctttg gcaaagcaac ggtacctctg tcgtggctgt
     3001 cacacctatt ttacggcgaa tacctacatt gttgaaaaaa actgttttat cgccaaacaa
     3061 gtacattata aaatattgga ggaattgact gaaaaacaag caatgacgac gatttctaaa
     3121 cattgcggcg tttcttggtc aaccgtttcg cgaacactcg cttctctgct ccccatgaca
     3181 aacgtgaaaa taaattggtt gcctcgctgt tcattggtag atgaatttcg ttcattgaaa
     3241 aatcaagtcg gtccctattc tttttcttgt atggatggcg atactgggaa attactggat
     3301 attcttcctt cacgtaagaa gaaagatctc atttcttatt ttatgcaatt tgaacgacgt
     3361 gcacgtttaa acgtaaaaat actcgtaaca gatatgaacg cctcttatgc tttgctgata
     3421 aaagagtgct ttcctcatgc caaactcgtt gtggatcgct ttcacatcgt caagcacctc
     3481 attcgcagtt ttaaggatat ttgactatga gtcatgaaaa gcttcgaccg aaacgatcca
     3541 atacaagcca aacactatcg acaagtgaag gctttatctc gacttctgat caaacgacag
     3601 gacacgcttg tttatgataa atggacaaaa tggcgtaatt ttggttgggc ctatttaacc
     3661 gaaagtgaag tcgtagaacg tctactttct acttctgatg aactacgaat agcttatgct
     3721 tactatcaag aaattcttca agctttttat gacaaagaag cggacatctt ctttcagctt
     3781 gtaaaaacaa tgcccaagtc ggttccaaga gaactgcatc atataaaaaa agcgtttata
     3841 aactatgaat ccggcattcg tttagcctta gagttgcctt actcaaatgg caaaatagaa
     3901 aatctgcata cccacatcaa agcattgaaa cgaatagctt acgggttagg atgagaacca
     3961 gaattttctt aatcaatcat ctgatcactt attaaacata aaaaaacatc taaagcagaa
     4021 aatcaattct accttagatg ttcttatatc actctatcag tcctatttga caaagagcca
     4081 gaaatgtttg ccgactatac actagctttg ttgtgcaaaa tgctgagtat caatttccac
     4141 agggattgct agaatatctt tcagctgaat gtataaggca tcgctaattt tctcttcccg
     4201 atcgttcaac cgatccacta aaatttcttt gccctccagc gtttgaaaaa cataccgttg
     4261 aatgttgcct aaaataaatg attccttcca tagaaccgga atcttaaccc cctgaggaac
     4321 cggttcaaat tgaatcagct ccggtcttaa ataataaatg cagcgattgc ccttagcgga
     4381 aaactcgtgg gtatactgat tcagctcata ccattcgaaa cgattatagt tgccgataaa
     4441 ttccgcaaca aatcggctct gaggattctg atacaattcc ttcgaggtgg aaacctgagc
     4501 cacccgccca gcttccatca caaaaatccg atcactgatt cgcatcgcct cttcttggtc
     4561 atgagtcaca aaaatcatcg tcatccctag cgattgctgg atagcgcgca aatcccgctg
     4621 cagctgtttt ctaattcggg catccaaagc acttaatggc tcatccagca gcaggacttt
     4681 cggttcagtg accaatgagc gagccagtgc cacccgttgt cgttgaccgc cagaaagaaa
     4741 ctggacattt ttatctgcct gctccgtcat atccaccaat gatagcatct caaaaacctt
     4801 ttcctgaatc acctcaggac tcagcttctt taccttcaaa ccaaaggcta cattttcaaa
     4861 aacagtcata gttggaaaca atgcatactg ttgaaaaaca aagccgatat tgcgatcctt
     4921 cgtaggctta tcggcaatat tctcattgtc cagcacaatc tcaccgctct ttggttgttc
     4981 caatcccgtg atacagcgca gcaacgtaga ctttccgcac ccgctgggac ctagtaaagt
     5041 aaccagctca cccttttcaa tctgaatttc atcaatagct aaaatctgtt tttcatcaaa
     5101 aaacagattg agatcatgta tttctaaaaa cattttccgt cacaactcct ttaagggctt
     5161 ctttttcgac tgcttctctt ccctcgtctg cttaagggac agctgttgct tttgtcgata
     5221 agtcagcata aaggtagtta aagcaattcc caccataaac agcaaataca aaaccatcac
     5281 agcgctagcc aaatggccgt tttcgttcat ccgccgcagc atatagatgc gtaccgtctg
     5341 aaatcgaccg ccaatcaaca ggttcgtcag catgtactcc ccaaaaaccg aagaaaaaat
     5401 catcagagaa actagggtca caccgatacg gatgtttgga aacagcacct ggataataat
     5461 cgtagacatc ggggcgccca aggtttctgc tgcatccact aattccttca gattaattaa
     5521 gcgcatctgg ttgttaagac taagatagac aatcggcaac gccgtaataa acaatgcacc
     5581 taccaacact acaaacattg ggataccagt ttcagcatac gttcgaagca aagccgttac
     5641 caaaatcact cctggtaaag catacggcag caggagcagc ttttccagcc aacgattcaa
     5701 acgagggaaa tacaaatgaa tccaaataat ggtgggaatc atcaacaaca gaatcgtcac
     5761 caagaccaca cctgctaata aaagtgagcg acccaccgca gcaataaatt ccctgtccgt
     5821 cagcagctgt tggaaccatt gcaaggtaaa atcactaggt aaaagcgatt ttgaccattt
     5881 agtggagctg gcatacaaaa tcgttccaat aatcggcagc aagtaataca ggacaaagca
     5941 gaacaaaatc ggttttatta actttttcac gtctgcacca ccttgccttt cttttgaata
     6001 atcaacattg ccgttaccag caggacacct aaagcgactg ccaacgcact gcctaaatta
     6061 ggcttggcga acacatctcc cgacactaaa gccgcaattc gaatcgttaa aagatttaca
     6121 ttactgctgg tcagggcata cgctgtttca taggtcccca tgccattagc aaataagatg
     6181 acaaaggtgc tggcaatcga tggtcgcaaa aaaggcaaag ccaccttgaa ccaagcatac
     6241 gctgatgaag cgcccagcaa ttgtgcggac tccagccatt cctttttcac ttccttcatg
     6301 ctgggataca aaaacaagac ccctaacgga atttggaaat aggtatacgt gatggtcaat
     6361 ccccaccaag aatacaaatt gaagtccgct agaaatggca aaatcatttt cagcacacct
     6421 acattcccca acaaaataat aaaagagaat gccagcggaa tcccagcaaa attagctgcc
     6481 aaattggaaa caaaggtgat tttctcctgc aatttagtcg atagattcat taagcataaa
     6541 cataaaataa acgtcccgat taacccaatc acactggagc tgacagcaat cagtacgctg
     6601 ttaacaaagg cctgtgtgta atatttgttg ctgaaaatat cactgtaatt ggccaatgtc
     6661 cactggttag ttaaatcact gtgcaagctg cacagaacca taaaaagcag cggcagcagt
     6721 aaaaatgcac agaagataaa ggaggcagga accagaacca agcgtttttt catctctttc
     6781 atcccctcat caattcttga atgtcctgca ttttctctga cggtgcaatt cctaataacc
     6841 agcaaaacaa tggagccgcc agcagctgtg gtacagtttg ttccttcagc ttttggcgtt
     6901 tatttgaaat caaataaaaa ggaacttcac gatgtgcagc caagctgccg ccatgcagtc
     6961 cgtgcgcatc catcccgtga tccgcagtca ccacgacttg ataacctttt tgcagccatt
     7021 caggaaggta aatagccaat aatctgtccg cctgattaac cgctgctgca tattcatgag
     7081 aatcggcggt aaatttgtga ccgacatcat caatattcat cgaatgtacg accaaaaagt
     7141 ctggctgata ctgattcact aaataatggg catccgcaaa taaatgcgaa tcagggtaag
     7201 tgtcctcact ataaaagatg ccatgctgaa tcagtcgctc ctcctgatgc tggatgcgat
     7261 caaccaaacg attatagggg gcttgattgt acaactcgct gtaccagtgg taagcggcgg
     7321 ctcctgtttt tccgccagct tccttaacta attcaaacag cgaccactct ttggatcgct
     7381 gagcatggta attactatag atgccatttt cataggctgg aacgccggtc atcagtactt
     7441 cgtataacgg acgtgaattg ctgggcagct cactttttac cttcaataag cttgcttgct
     7501 ggtgctcaac taaatgattc atgaacccaa gctgggcgac ggctgtgtcg tagcgacagc
     7561 cgtctagtat aactacaatc agtttgtcaa gtctactttg cataggtcag tacctccgcc
     7621 tgccaaagct ctggaagctc tacagaggtc ttgttccaag cttcattgtc ttcaactggc
     7681 accgccgctt cataagcatc ctccggcagt aatttttcag ctacttcttt aggcagctct
     7741 accttgcgaa taggtcgcgc atagcccttt gccaaattga tttgcccttc atcagataaa
     7801 atatattccc gtgctagctt tgccgcattg ggatgagggg catttttatt gattaaggtt
     7861 gtatacccgc taatgatgga gccgtctgtt ggcacagtta cctcaaaacg ctcggagtca
     7921 atttggtcac gataatttag tccattgaag tcccaaacaa ctgccacatc gatttcacct
     7981 ttttcaagat ttgctacgct ggcatcaacg gtggacaaac ggcctttttc tgccattttt
     8041 ttgtagtaat tcagacctgg ctgaatatcc ttttcgtcac ctccgttggc aatcgccgca
     8101 gccaatacag caaattgtgc ttggttggca atcgtcacat cccccacgct aacttgatag
     8161 tcaccctctt gcagctcctt ccagctagtc ggtgcttgct tcacattctc tttatcggtg
     8221 ataaaggaaa tggttcccgt atagcttaac aaccaatccc catcatcatc ctttgcccaa
     8281 tccggaattt cctcccaata gttggtctta taaggtaagg ttaactcttt gctttcagcc
     8341 aacggaccga agctgatacc gacatcacca atatcggcgg ttccattttt tccttctgat
     8401 tcaaacttag ccaattcttc tgcactggac atatccgtat ccacgtgact taagccgtat
     8461 tctttttcaa ggtcgttcca agtgcctacc caatttgccc aagtatccgg cattcccaag
     8521 ctggcaatct ctccttcttc cttggctttt tctataatcg tatctaaaga gtcgtctgcc
     8581 gcagccgatt gcttcgttgc ttcacctgag ccgcagcctg tcagcaggat acttactgtt
     8641 gctgcacaaa ataaaccgat agtcatgtta cgaatttttt cattcatttt cattcattct
     8701 tctccccttt cgataattcc attctaacaa agctttgtaa tgagagaatc agtttcttga
     8761 aaaggtttca tgaacaatgt aaaggttgta tatggtttgt aaatttcgac gaattcccgt
     8821 taaccttcta aagcttttgc ggtatgatag tagaaaaaag gaggctccct atgcagtatt
     8881 atgatcagca tctccacacg tatttttcct ttgattcaga ggaaaaattt gaaaactatt
     8941 tagcctatca gccggagttt ttcgtttcta cggatcactt tgatttaaac aatccatcta
     9001 ccggaaaaga tgacattccg gattatccag cctatataaa caaactggac tctttaaaag
     9061 aaacctattc caccaaattt ctgcgaggta tcgagattgg cgttgttcca ggacaagaag
     9121 agcggatcgt ggattacctc gctcagcatc cttatgattt aaagctggta agtattcatc
     9181 aaaatggccg ctttgattat atggacgatg tggttttaac caaagatccc attcaaacta
     9241 ccaaggaata ttttgatcag atgttgcagg tcttgacgga ttttccagaa ggagatattt
     9301 tgactcactt tgattatgga ttacgccgct tcaccttcac agcggaagaa ttcgcggagc
     9361 attttgaggc tcagctgcta acgattttca aaaaggttgt tgaacgagaa ttggcgatgg
     9421 agttaaatgc caagagcttc ttgaaatacg gcaacgaaga cctttaccgt tatgccgttc
     9481 ctttgtatca aagtgtcggc ggaaagctct ttaccttagg ctcggatgct catgttgccg
     9541 aagattatca gctgggcttt aaaaaaatga gtcagctgtt ggcagactgt caggtagaaa
     9601 agttgctagt cattcaagga aaagaacgcc gcctgacgcc gctgccgatt ttgtgattcc
     9661 tatataatag aagaaagtgg tgaaaaaaat gagcaacctg tgggactaca atcaagaggc
     9721 acccatccat tatttaattg cgcgacattg ggatgcgttg aaaattgagg cggtttgccg
     9781 atccttgtta gcagccgttc caaaacaaca actggaaaat tttttggtgg ctgacagctt
     9841 gcagcgggaa aaagttcagg cttattttgc agcctttaaa gatcagcctt tggaatattt
     9901 gcatgcgcag tttcatcttt tttatcaagt agccgcccca gatgactaca acgatttacg
     9961 aggacagctg caattgacct ttcaagcgga tgaaaccgcc tataccgttt tattgggaat
    10021 ggctcgcctt ggtgatcagg cgaaggtcga gtggcggatt ttcgatattt gatggcagga
    10081 tcaaggcgtt tcagttgcaa accttcctct ctttcaatta gaaaaaccgc agcaggaaga
    10141 agtttgtgtg ctggtcaccg atgctggtga aattcgtctc cgcttatttc ctgaccaagc
    10201 tcccctggct gtagccaact ggcgctccct agctgagcaa ggcgtttatg acaacacacc
    10261 tttcggctcg tgtcattaag gaatttgtta ttcaaggtgg cgctttggat ggttctggtg
    10321 atgaagctgt ttccagctat gagggctttt tcgctgatga agtgcatcgc ggactttatc
    10381 attttaatgg cgccttggcc ttaggggacc acggccccca caccaacggc aaccagtttt
    10441 ttattgtgca aaacacaaag gcgcaggctg acctgctaat gtaaagcaag catacttgga
    10501 gcttggagga ctaccggagc tggatggtcg ttatactgtt tttggacagg tataccaagg
    10561 gttagaaatc gtagcagcga tcgcagctca gccgacaaac agtgaagatg taccgattga
    10621 gccagtgttc attcgacaaa ttaacttcga aaaaacatcg taaaaagaca ctcactgact
    10681 tcagcaaaag ctctattctt gagctggctg cagctagtga gtgtctttca tttatccata
    10741 agataaagct tggattcaat aaataaataa atgttttggt taaagctgtg accagcaaat
    10801 gaggaaccgt gcgagccagt tgatctgcca atgcaaacat ttctttaggt gaaggcgctg
    10861 gcgtgtctgc caacggatac tccagtccca atgcttggta accgtgcata ccatagcagt
    10921 ggtacggtag taattctaac ggtacgaacc gatcaagatc tgccacaaaa caagcaatcg
    10981 tcagcagctc ggtcaggttc atattgatac ctggaaccac aggaactcga attcgcactt
    11041 cgcagttagg atactcaatt aaaaagcgac gaatattctt caaaatttgt tggttatcta
    11101 agccggtcca ttgtttgtgg agcttgctgg taaacatttt tacatccaca aacaatgtat
    11161 ccacataggg agctaccttt tggatttcat tataagcacc acaaaagctg gtttccagta
    11221 cggtattgat tccaatgtat ttcgattttt gcaaaatttc tttagcaaaa tccgcctgaa
    11281 ccagtgcctc gcctccgcta atggtcactc cgccatccga gtgaaagtaa aaaacagcat
    11341 ccttctcaat ctcatccatt acctcttcag cggtcatggt ctggccaaat cctttttcaa
    11401 tctgcttgct ttgtgattcg ggaatcgaac accaggcaca gcgtaatggg cagcccttta
    11461 aataaacaac ggtccgtaaa ccagcaccat catgtagaga accctgttct attcttaata
    11521 ctgttccttc catatagatt tttgaattac acactagttt aaactaaaac acacaaggaa
    11581 aaacttacca tttaatcata aaattagctc atataaatat actaggatgt aaaatagact
    11641 ggtcaaaatc tggtcaaaaa agaggactaa tacaggtagt cctctttttt gttattttaa
    11701 tattctaaat cattttttat tggtttattc ccttattcga tgtagactca aatcactata
    11761 tcactagata tcaaacggcg tcatacggaa tcaaaaacat caaatttgac ctgttaaatg
    11821 gtcattgaat aacattcaaa atcaataaaa cggagtcaga cacggagtca aaaacgctga
    11881 gagtaaaaaa tatatacagc cttgtaggta aagtaccaca gggctgtata ttttttattt
    11941 cagattacct taaatgattc catttacttt ttatctcatt ttttcctact agttagtatc
    12001 aaatactctc ctattttctc aataaactat ttttggatac atggtatctg aaacagctaa
    12061 aaccttgtca ttacagcaaa aaatcacgtt a
//
LOCUS       DACCQI010000089        11930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCQI010000089 DACCQI010000000
VERSION     DACCQI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.092"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            50..259
                     /locus_tag="IVO76_002080"
     CDS             50..259
                     /locus_tag="IVO76_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430028.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            466..666
                     /locus_tag="IVO76_002081"
                     /pseudo
     CDS             466..666
                     /locus_tag="IVO76_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1378..1983)
                     /locus_tag="IVO76_002082"
     CDS             complement(1378..1983)
                     /locus_tag="IVO76_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7430029.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2165..2500
                     /locus_tag="IVO76_002083"
                     /pseudo
     CDS             2165..2500
                     /locus_tag="IVO76_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2665..2892
                     /locus_tag="IVO76_002084"
                     /pseudo
     CDS             2665..2892
                     /locus_tag="IVO76_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3018..3989
                     /locus_tag="IVO76_002085"
     CDS             3018..3989
                     /locus_tag="IVO76_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7430030.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4129..5442
                     /locus_tag="IVO76_002086"
     CDS             4129..5442
                     /locus_tag="IVO76_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7430031.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5485..6351
                     /locus_tag="IVO76_002087"
     CDS             5485..6351
                     /locus_tag="IVO76_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7430032.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6348..7196
                     /locus_tag="IVO76_002088"
     CDS             6348..7196
                     /locus_tag="IVO76_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7430033.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7210..8682
                     /locus_tag="IVO76_002089"
     CDS             7210..8682
                     /locus_tag="IVO76_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7430034.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8699..9571
                     /locus_tag="IVO76_002090"
     CDS             8699..9571
                     /locus_tag="IVO76_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7430035.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9807..11189)
                     /locus_tag="IVO76_002091"
                     /pseudo
     CDS             complement(9807..11189)
                     /locus_tag="IVO76_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11651..>11930
                     /locus_tag="IVO76_002092"
     CDS             11651..>11930
                     /locus_tag="IVO76_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430036.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT         3700 a         2037 c         2401 g         3792 t
ORIGIN      
        1 tttaaaaaga aaagtgacag ataggaataa aaacttatta aatgtagtta tgatctgtta
       61 tatttcattt ctaatactag gtcttccaca agcagtgttt cctgaaatgt tttctggaga
      121 ctggggtatt gtcagctatt ctatattaac attgattgct atggttttag gtacttcatt
      181 ttataatgct tttgtaagta agcatgttga ggaatatgaa gtattaatgt acttaaaaga
      241 gaaaaaaaca agtaattaat atgagaacac cacgcatagt ttttattttg agatattcta
      301 gaatgaaaca cattaagaac gaaagcaaag caaaagacgc cagaacttct actaccttct
      361 aagttttttc atatttgttt gttatgctat acatagatct gcataattag acttgcgctg
      421 cccgttattg aagtttcagg cagcgctctt tttgggggat cgataatgga agaagaaaaa
      481 atatttgaga aacgctggca gctagcatca agtgaacaaa gagcaagata caataatttg
      541 atcacctcct accccactgt tgactagaca tataaggaaa agaaatactt actttggctg
      601 tgccagttag atatagatac ctttgaaaca tttgaagtga tcctagataa aattaaacag
      661 aattgatcag aaacaagcga agccttctct cggttcgctt gttttgctat ttattagttg
      721 gctcagggca actctaagct cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac
      781 cactttagaa tacatggatg tacgtattgc cattgactcc tattagttac tgaaaaaaaa
      841 tttaaacaag ttgagtattg cagataaata gctccagtga ggtgaataac tgctccactc
      901 aaggcaatag atagctccat ccagggcaac aaactgctcc actttcatat ttaatagatg
      961 aataaccttc tgtataatag aaaggcatac ctttcggtat gacaacttat acagaatgtt
     1021 attttattgg ttcactatcg aaaaattttg gagctttatt tttccggtat ttctcaacga
     1081 acgattagtt taactgtggg aagctccaga catacgattc gagaagttac ttctcgagca
     1141 gacaggaagg gggtgactga actaaccgct gatatgagcg atacttggct gtaggaatat
     1201 ctttttccgg aaaaacaaac aacagcacga ggttaccttc ctgtagactg ggaatatgtt
     1261 cataaagaac taatgaaaaa gaatatgaca ttgaaactcc ttcatagaga atatggggta
     1321 catggaacat agaaacgaaa atgcacggta gaaaagcgaa tgaatatagt ataaattcta
     1381 caaatcattc gctttcttta ttttatatat ggttggtcga gagagaccat tttctttggc
     1441 aatttctgca actggaactc cttgatttaa catgtccacc acagttttaa agactaatcg
     1501 tttttgcgga tctgttgagt tctcagaata ttcaattggt cgccccttat atcgaccagc
     1561 ttttttggcc aataatactc cttgccgctg acgttcacgg attttctcac gttcgttttg
     1621 tgcaatataa cttagcaatg ataaaaacat atcgaacata gcagtatcta ggagttcatt
     1681 tccagtatta aaatctaaaa actgtgcatc tagtattttt aatgctactt ttttctgttt
     1741 cataaaggta actgtatttt ttatgtcttc ataatctctg cctaggcgat ccagtgatgc
     1801 aacaatgaca caatcacctt ctcggatata atttagtaat ttttgaaact caggtcgctg
     1861 gatatcttta ccactggctt tttcttggaa taatttatca atgttaaggt cttccatcat
     1921 tgccaattgt cgatctaaat tttgatcttg tgaagacact ctaatatatc caatttttgc
     1981 cataaaaact ccttttaaaa aatgatataa tttacatgta aaatttactt gtagttttac
     2041 tgttatcata gcatttttaa aaataaattt taagtctact tataatttac tttggaggtt
     2101 ttgaatgaat gaaattaagc gcaataaaaa ttcttttggt gtttatttag gagagcctag
     2161 ctcggaacag ttaaataaag gtgaagctcg tcatgctcta tgtcgaaata tattttatgg
     2221 tagaaaaggc aaactttatc aaacttatta cgacggtatg gaggaacagc tcaacgcatt
     2281 aggattagta actaatgcta tcatatattg gaattcacta tatttagaaa aagtctttga
     2341 acaaattaaa acggaagggt tcgactgttc cgaagaagtg ttacgcaagc aatttccatt
     2401 aattagtgat cacattaatt ttgttggcaa atatagtttc cgctatgaaa aagaattatc
     2461 gaatggaata ctacgtccgt taaatataga gaattattga gaatgttcgt cttttggttc
     2521 ctaccgtgca ttttcgcacg gatgttccag atactatttt tgaatgtata aaattgaaaa
     2581 cacaagaata ctaataaatc aacgattctt gtgtttttaa tttttcacta ccgtgcattt
     2641 tcgtttctat gttccatgta ccccaatctt gaaaaattaa ttaaagactt agataaagcc
     2701 attgacgact tagtcgtggt gctaccggag tatcaatgtt taaccagtat cccaggtatt
     2761 ggtcctgttt aggctgctgg aatactcgct gaaattggtc caattgaacg tttttctgat
     2821 gaaataaaat tgacaaagta tgccggccta tattggcgac agagtcaatc cggtaaatct
     2881 gaatacgaaa atttttttac gaaaagcttc acgtctaata tctattttaa aataaaaata
     2941 ttcctgctaa tttggaatcg tttccttctt ttggtataat aatacctgaa attcttttga
     3001 gaggagttgt tcgtgaagtg aaaactacta tgaaggatgt tgctgcgtta gcaggagttg
     3061 gtgtgggtac ggtatctaga gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa
     3121 aagtaatggc tgcaattagt gagcttaatt atgagcctga tgaatatgcc agaggtatga
     3181 aaacgaatag tagcaataca atagctctta ttttacctac gatttggcat cccttctttt
     3241 ccgagtttgc ctactatgta gaggaagctc tcagccaaaa aaattataag ctttttttgt
     3301 gtaatgcgaa cggcagtgcg gaaaaagaaa aagaatatat ccaaatggtc aagcagagta
     3361 aagtagacgg aattataggt attacctatt cagacattga taaatatgta tcatcaaatt
     3421 taccatttgt cagtatagac cgccattttt cggaacaggt ttcttatgta acggcagata
     3481 attatttagg tgggcaaatt gccgctcaag aactacttac tcgtaactgt aaaaacttgt
     3541 gctatgttgg gggtgtttca ccttacccta acgaaacaaa caatcgtaaa gctggttttt
     3601 acaatatttg tgaggaaaat aaaatagctg tgaaggtatt ggatatgcct gaaccaatta
     3661 gcaatttaga ggaacaattg ctcgcttttt tttcggataa tcctgaaatt gatagcattt
     3721 ttaccatcaa tgatttcatg gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg
     3781 aagcacctaa ggattttcaa attattggtt ttgatggagt caagctatcg ttgcaaaaag
     3841 actatttact atcaacgatt gtacagccga tagaggaaat ggccaaggtt tcagtagaaa
     3901 cactcatggc gatgatttta aatgaagaac cgcctaagcg tgtggtagta ccggttttct
     3961 ttcacgctgg agagacgact aaagagtaaa aaaccggttt ataacgattt ttttgagata
     4021 ggttgacaga aagtgatttc aatgatatct taaaaccaat taattggaaa cgttaccaat
     4081 taattgaaaa ataaaacgga aacgtttcaa aaaataggag gaacatcgat gaaaaagaga
     4141 atgttagcaa ttgcagcaat tttagcatta acagctggag gacttgcagg atgtggcagt
     4201 aaggagaaaa gttcaggtag cagtgatcca ggagaaatta agatttgggt tcagttttct
     4261 gatgaaacgc ctgaaggaaa agcttgggca gagattgtga acaattttaa tgatgaatac
     4321 gagggtgaat ataaagctgt tactgagtat attcctcgta gcggaagcgg cggtggatat
     4381 gaggataaag taaatgctgc aattacaacc aatacccttc ctgatgttat tacgttagat
     4441 ggaccgaata ctgcagctta tgctcattca ggagtcattg ctccattaga tgattatttg
     4501 aaagatgcgg acatggatga tgtattagac agcattattc agcaaggaac ctacgatggc
     4561 aaaatgtatg cgtttggttt ctcagagtcc agtgtgggtg tctattacaa taagtcgctg
     4621 ttcaaagatg ctggaattac agaagatcaa ttgccgacgt tagaagatcc ttggacttgg
     4681 acggaatttc aagcaatttg ccaaaagctg actgataaat acaaaacgcc tgcaattgat
     4741 atgcaaatgg cgtcgggaga cgaaatgctg acctacgcat atacaccatt tatatggtcg
     4801 aatggcggcg atgtcgttaa tgctgatgga accaaagccg aagactattt taacggtaaa
     4861 aaatccgttg aagcgttgga atttattcaa gaattggttg aaaaaggtta caccacaatc
     4921 agtcctattg aaaaaggatt tgaaactggc aaatatgcca tgatgctgag cggttcttgg
     4981 acaatggcag atttagagga aaactatcaa gagattgatt atggtatttt gccttacccc
     5041 gtttcagatg caactgggga gttagtttca ccaaccggaa gctggcagtt agcaatgact
     5101 gagaaaacag agaaaaagga tgcttctgcg gcctttatta agtttgcaac cagtacagaa
     5161 tctagtcgta ttatgagttt agggaacagt gttctaccta tcagaaattc aacagttgat
     5221 ttaattaaag atgaagtgtc ggaaggaatg caggtattaa ttaagcaaaa ccaactttct
     5281 ggacatgccc gcccagtggt tgtagcttat ccgcaagtat ctagagcatt ccaacaagca
     5341 gttcaagata ttagctactt cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc
     5401 gctgctgaaa tgcaacaggc aattgatact tcactaaaat agaagtttct aaagggggcc
     5461 aatcaagtgt taaaaaaacc gacaatgaaa gacaatttga taggttacgg ctttctagcg
     5521 ccagctttgc tgctgctgac gatttttctg atcatacccg taatcatggt aatttattat
     5581 gcctttactg attattattt gttaacgcca gatgctagac aatttatcgg cttagaaaat
     5641 tttaagcgac tgttcgctga tccaattttt gtaaaaagta tttggaacac ggctaaattt
     5701 gttgtttgga ttattccggt ccaaataggg gcagctttag gaatggcact tattatcaac
     5761 aaaaatcgta aaggaaatat cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc
     5821 ttagtcgtta tttcgatttt atggttgtac ttgttaaatc ctaatgaagg tctggttaat
     5881 acgctatttg caaagattgg aattgcttcg cagccttttt tgactagtcc caaacaagca
     5941 atgtatacgc ttgttttcgt atcggcatgg caaggtgcag gatatcagat gctgctgttt
     6001 ttagggggaa tgcaaaatat tccgcaagac gtttatgagg cagccgaaat tgatggtttt
     6061 tctaagtttc agcaatttcg ctatatcacc atgccattat taaagcccac ggcgattttt
     6121 attttactta ccacattgat ttcggccttt aaactaatca ttcagccaat ggtaatgact
     6181 caaggtggac caatgaattc tactatgaca atggtttatt acatttacca aactggattt
     6241 acagaccgta tggtaggcta ctccagctcc attgcgttaa tctttaccac tatgattggt
     6301 ttaattacaa ttgctcaaag aaaattggta aaggaggatg atccagcatg accaagaaat
     6361 ttggaccgct aatgatcgta gaatattgtt tgctgttcct tttagcggca ttattcattt
     6421 tcccgatgct gtggatggta gtttcctcta tgaagcctga agcagatgtc tatcaaaatt
     6481 tagcttcctt caaagctttt ttacctagtt tgaatccagc taattggttt aaaacttatc
     6541 aagaggttat ttcccggttc agtgtgggaa cttatttatt aaacagtatt ttttatggtt
     6601 taacctttgc ctgtgggtca atcattgtaa actctttggc gggttttgcc tttgcgaagg
     6661 ttaatttttc aggcaagaaa attatctttg gagctttgtt agctctgctg attgtcccga
     6721 tggagacggt tttaattccg caatttacca tcgttaattc actaggctta gtgaatactc
     6781 gcttggcagt gattttacca gcgatggcca gcgcgtttaa tatctatctg tttagaaatt
     6841 tctttatcgc gattccagaa gaaatcattg aatcagcgaa attagatggt gcaaatattg
     6901 ttaaaatttt ctttcacatc atgctgccaa tgtctaaacc agccatagcc acagtaggtg
     6961 tcttatcctt tatctccagc tggaatgatt atatttggcc gttaatggtc ttaactgaca
     7021 gcagcaaatt ttcaatgcaa gtggcaatta ctacgatcaa tactacacag ccagtttata
     7081 tcaatcaagt gatggctgtt ttaaccattg caacgattcc actgattatt gtttatatcg
     7141 ttgcacaaaa atatattctg cagggacttg gcggttctgg aacgggtata aaataggagt
     7201 gaatagtgaa tgttgcaaaa acttttaaag gccaatgagt ttattgagaa aaatgctcaa
     7261 acagtcaatc aagaataccg gaataaattt catttgatgg ctccgattgg ctggatgaat
     7321 gatccaaatg gttttgttta ttatcaagag gagtatcact tgttttatca atattatcct
     7381 tacgacagca tctggggacc aatgcattgg ggccatgcta aaagcaagga tctaattcac
     7441 tgggagcatt tgccagtagc actggcacca ggtgaaagct atgacaagga aggctgtttt
     7501 tccggcagtg ccatagaaaa ggatggcaag ctgtatttga tttatacagg acacaatgtt
     7561 gtggaagggc aagttcgaca agttcagtgt ttggcagttt cagaagatgg cattcacttt
     7621 gaaaaatatg ccggaaatcc aatcatcgca gaagatcact tagcttctgt tgcaacgacc
     7681 gaagatttcc gcgatcccaa agtgtttcag cgaaaagaag actattttac tgtagtcgct
     7741 actaaaactg cagatgctcg tggacaaatt gtgatgttcc attccaaaga tttactaact
     7801 tgggagtttt attccgtatt gctggaggga actccagaac aaggcattgt atgggaatgc
     7861 ccagacttgt ttgaactaga tggcaaagaa gtcttgattc tttctcctat tcaaatgact
     7921 ccacaaggca atgcttacca aaatacgaac tcaactgttg ctttcatcgg tactgtcgat
     7981 tggcaaacag gcaaatttct agtagagaat tttcatgaaa tcgatggcgg tttagatttt
     8041 tatgcacccc agacctgtga aaacgaagca ggccaaagaa taatgattgc ttggatgcaa
     8101 atgtggcagc gtaaaatacc tacgaatgat ctgggccatg gttggtcagg ttccatgacc
     8161 ttgcctagag agctgagggt ggctgataat caattgttgc aacggccagt ttcatcaatt
     8221 tatcaagtaa ttgatccgca ggaaaggatt gaatgggcga agactgaaga gaatcgcttc
     8281 ttactgaagg acttagtata tgaccagcac tatttacgct tggaaatcaa tttaaaacag
     8341 gcaacgaaac tagaacttca ctatgcgaag ggacccgacc atacattact gcttaactat
     8401 gatgttgaac gggagtgctt tactgcttcc agggaacaca tgccgtatga tttatcagga
     8461 gctgaaaagg aagtgttgaa aaagcgcagt gtgacgattc ctttggaaaa taattgtcta
     8521 accatcgaaa ttttccgtga tacttcagct attgagcttt ttctaaatga tcgtctgact
     8581 atgagtacaa ctttttatga gacagaaaaa ggacaggatt ttcttttgct ggcagacagt
     8641 gaagtaacct tttcattgga aagcggctta attaatctat agaagggaga gctcagtgat
     8701 gatttatggt tcaatcgaag caggcggcac aaaatttgtg tgtgccatta gcgatgaaga
     8761 aatgacgatc aaggaaagag tgagctttcc gacaacgaca cctgaggaaa caataccttt
     8821 agtcattgac ttcttcaaac aataccaagc agatttagca gggattggga ttggctcatt
     8881 tgggcctatc gatatccatc gcgattccgc cacttatggg tacattacct cgaccccgaa
     8941 agtggcatgg caaaattttg atttcatcgg taccatgaaa aaagaatttc cgatcccgat
     9001 ttcttggaca acagatgtca atgcggctgc ctatggtgag tatgtcttcg gcagtggcaa
     9061 aggattgagc agtgtcgttt actatacgat cggtacgggt gttggcggcg gtgccttaca
     9121 agaagggcgc tttatcgaag ggtttagtca tccagaaatg gggcatatgc tcgtcgtccc
     9181 tcatccaaaa gatgactttg ctggcagctg tccatttcat ggcaactgtt tagaagggat
     9241 ggccgctgga cccgccatcg aaaaacgctt agggaaaaaa ggacaagagg ttgcagaaga
     9301 tgatccttat tgggaaatcg aagcctccta tattgctcaa tgtgcctaca atacaacctt
     9361 gatgttttct ccagatgtga tcatttttgg cggtggtgtg atgaaacaac gccatatgct
     9421 gcaaaacgtc cacgacgcat ttgcaaaatt ggtcaatggg tatgtcgaaa cacctgagtt
     9481 aagcaaatat atcgtgaccc cagcattaga agacaacgct ggaacgctcg gttgtttggc
     9541 attggctcga gaagcggtct tacattcata aacgaaaaaa ggctcttcga catttagtgt
     9601 tggtagagaa agatacaact gaaaatttag tcataaccag ttaggcagag tgcttaactg
     9661 gtttttgttt tacttggaag atcaagcctt gaatgagata agttcttgct cgaaagttat
     9721 ggaaatttcg atagccgtag gctacttgta ttgtgcaata gttttttaca aaaaaaaggg
     9781 ggtgcggata ttttttttgg acctttttaa caaatatatc tttgcttctt ccgatattga
     9841 agagggctta gccttcctaa agataactta attcgttgat tattatacca atgaatataa
     9901 tcatccaatt tgtttataaa ctgattgatt gttgtattct tccagtcacg ttgataaaac
     9961 atctcgtttt ttaatcgtcc aaagaaacct tcgcacgctg aattatctgg tgaataactc
    10021 tttttagaca tagagtgaag caagttggct ttgttccaat agtccaacta acaatagcac
    10081 catcgtaaca atcgacaagt ggtgataagt agattttccc tgcgagaatt ctaaattcag
    10141 taatatctgt taaccatttt tcatttggct tctttgcttt aaaattccga tttaataaat
    10201 tggggtgtgc aggagaaatt tccccagcat acgaagaata cttttttcgt ctagaaaatt
    10261 taacaaatag attttcctca gtcatcaatc tacgtacaac tttttcagaa agctttttac
    10321 ccatattttt taaagcttgg tgaattctac ggtaaccata cgcacagaag ttatgattga
    10381 aaatcgtgat aattaatttg acgttctttg taatacttat ctggtttctc aagtgcttgt
    10441 ttttgataaa aatagctgct tttaggaaac tggagctgtt gaagaagctc tttcaaggtg
    10501 aactgatttc gtaaggcatc aatcaatatc gttttttctt gatttgatag acaaagaaga
    10561 ttgacgcctt tttctttttt tagcagttca gccgctcctt cgagtaatgc tttttcgagc
    10621 tgtaattggt gtacttgttt ttgtaagtct tctacttcct ttaaaagtag ctctttttgt
    10681 ttttcctgat tttttaaagt catacacaat ggaaaatcct ttccaagaac cgtgtttttc
    10741 cattgatata gagtaactct actaattcct ccagattcgg ctaaattttt cctattagtt
    10801 tgcggtttat agaatttttt caacacagct tctttttgat cttgtgttaa ctttaccgca
    10861 ctttttcgga gttttctagc tcctggtgca agttcttcac accgctgccg caaggactct
    10921 ctattaggat aaccaagcat gcgaatggtt cgtgcataac attgaccata ttcaaaataa
    10981 tgattcaccg ctattcgttt ttgttcttca gaatactttg atttttttcg gtgctcctga
    11041 tggagctctc cggattccaa atactcttta taccatttcc gtagtgcacc aatcgacgga
    11101 taccctaatt ctcgaatggt tgcagcataa gagcaatcgt attttaaaaa taactgaatt
    11161 gcccttacac gctctttgtg ggaaaacaca cggacattcc tcctaagttt attttttcgt
    11221 ccaggattat gtccgcaccc ccttgctttc tgatgactta ttctggttct ttgagtgtca
    11281 atgcatagat gtgatgtagc tagttagaaa aaaattttga atactctttt tttctttcag
    11341 actccttttt aagtaagatt ttgaagtgtg agttacgggg tcagaaactt aaaagttttt
    11401 gatcctttat tttcaagtct gatttggcag cttttcggca gctcatttat ttatggtcag
    11461 aaatagatgc tgaaatttta atctattagc aaatagtcca gaacgtatga agaggagtcg
    11521 gatatcattt gtcattaaca ccataaatat ttataaaatt cgtgttcacc tacaattaaa
    11581 tttaatatat aatattcgtt gtaagggctt ataatttttt gttaatgaac aagataggaa
    11641 gaaacattta atggacagtt ttcagagatt gtcactagta aatcaatttg agttattggc
    11701 taaatttgag gaccaaaaca gatcttatta tgaaagaaaa atcgaaatat tgagagaagg
    11761 ttacgagtat cattatgatg atgaaatttg gagtgatctt tctgagccat ttccgaaaga
    11821 agattctcga tttgtattag atgtattgaa catgtatcga gatatcaatt ttagtaaatc
    11881 aaagctaaac ttagatgaaa accaaattac taattcatac tatactcatt
//
LOCUS       DACCQI010000090        11510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCQI010000090 DACCQI010000000
VERSION     DACCQI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.094"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(79..2154)
                     /locus_tag="IVO76_002093"
     CDS             complement(79..2154)
                     /locus_tag="IVO76_002093"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7430037.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDIKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKDHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEV
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IVO76_002094"
     CDS             complement(2156..3073)
                     /gene="glyQ"
                     /locus_tag="IVO76_002094"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7430038.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRF
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARSVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IVO76_002095"
     CDS             complement(3457..4269)
                     /gene="recO"
                     /locus_tag="IVO76_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7430039.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFLSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHAYPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKMPEKNMPEKEIPEKEE"
     gene            complement(4412..5311)
                     /locus_tag="IVO76_002096"
     CDS             complement(4412..5311)
                     /locus_tag="IVO76_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7430040.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYTALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5326..5727)
                     /locus_tag="IVO76_002097"
     CDS             complement(5326..5727)
                     /locus_tag="IVO76_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7430041.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQIDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IVO76_002098"
     CDS             complement(5705..6181)
                     /gene="ybeY"
                     /locus_tag="IVO76_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7430042.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6198..8390)
                     /locus_tag="IVO76_002099"
     CDS             complement(6198..8390)
                     /locus_tag="IVO76_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7430043.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNTGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8414..9385)
                     /locus_tag="IVO76_002100"
     CDS             complement(8414..9385)
                     /locus_tag="IVO76_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7430044.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10090..11469
                     /gene="brnQ"
                     /locus_tag="IVO76_002101"
     CDS             10090..11469
                     /gene="brnQ"
                     /locus_tag="IVO76_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7430045.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3107 a         2419 c         2104 g         3880 t
ORIGIN      
        1 ttatttatat ttataacaaa taaaccagag aaatagccta ttttgtgcct gttctctggt
       61 ttatttacat attagaaatt attttgtgat gatttgatct aaacttgcta gtgacaaagc
      121 catggcagca atttgactta attgcgttaa tcggttctta cgtactgctg gttcttctgc
      181 catgaccatc gtctgatcaa aataattttc gatcagtggg cgaaggctta caagctgatc
      241 ataattttcg ttgatcgttt ggtcagcaaa gcttgttttc attacctcta ctgctttgtg
      301 cagttctttc tccgcttcgt tttcgaacag ttcagggtct acttctgatt gtttttcaag
      361 ctccgctttt ttcgctaagt tcacgacacg tgtcaaagct tccatcgatg gtttgaagtc
      421 ttcatctgca agatgctgct tgaacaattg tgctgctgca aatacttttg tcagatcttt
      481 ttgttctgcg ttcaaaacag cttcgatgac atcatgacgg atattcttcg ttgtcaataa
      541 ttggcgcaga cggcctttga taaagtcgat cacctgttgt tgtcccgctg acaagccaat
      601 gccaaaacga tccgtatctt tactgataac ttcactaatc gtttcttgca gaacagacaa
      661 aggaaaggcc cattttttcg attcgatgat tcgaacgaca ccatatgctt gacggcgcaa
      721 agcatatgga tcgtttgagc cacttgggat catcccaact gcaaagaaag aaaagatact
      781 atctaattta tcagcaatcg ctaaaacaga acctactgta gtctcaggta aatctccttc
      841 actggatata ggcatgtaat gttcacggat cgctgttgct acggctggtt tctcgccttt
      901 aagcagagca tatttttctc ccatgatccc ttgtagttca gggaattctc ctaccatgtt
      961 ggttactaga tcaaatttat agatttcagc agcgcgttgc agatccatca attcttcctc
     1021 agacagtcct acttgttcgc ctatgatgcg agcgatgact tgtacccgtt gcattttctc
     1081 atagatgcta ccgatttttt catggaaagt aacagttttt aatttttcaa cgtattggtc
     1141 gattgaagct ttcttgtctt cattataaaa gaattccgca tcttctaaac gagcagtcaa
     1201 tactttttga ttgcctcgta ccacattttc caattttacc cgatcgccat tacgagcaga
     1261 aataaaatga ggaagcaaca taccttgatc gttccgtact tcgaaataac gttgatgttc
     1321 tttcatagaa gtgactagta cttcttcagg aacagataga tatttttctt cgaagtcgcc
     1381 agaaaagaca gttggatatt cgactagatt gttgacttct tctagtaaat cttcatctag
     1441 atcaatgatc cagtgatctt tttctgctaa ttcgtttgct tgtgtgatga tcatttcttt
     1501 tcttttttgc ggtgaagcaa taacaaattg ttcatttaat ttttcttcgt attcttttgc
     1561 atgcgcaaat gtcacatctt cgcctaagaa acgatgacct cttgaagctc tgtctgtttt
     1621 gatatccaag atctcaaacg ggattacttc gtcatctaac aatgccacga tccagtgaat
     1681 aggacggata tattcaaaat cgtattctgc ccaatgcatc gttactggga aagtgaggct
     1741 tttaatgaca tctgctaaac ctgtcaatac ttcttttgct tctttgccgt gagtgtattt
     1801 cgttacatag acatattcca cgccttttaa ttctctaaaa gtgatagcat ctgtcgtcag
     1861 accttgtccg cggacaaatc cttccgctgc tttcgtccag tttccatctg cgtccaaagc
     1921 aatttttttg gctggtcctt tgacttcttc ttcactgtct gcttgacgtt ctggaatgtt
     1981 tgttactttg attgctaaac ggcgcggtgt agaaaatgtt tcaatcgtat cataagtcaa
     2041 ttggttttca tctaaaaatt ttgctgtttt ttcttctaat tgtttcatgc ttggtgtgac
     2101 aacatgagca ggcatttctt ccagcccaat ttcaagtaat aagtcttttg ccatcttatt
     2161 cagcctcctt cacgttttct ggatttgctg tgtttttatt caataaaggg aatcctaatt
     2221 tttcgcgttc tgccacaaag attttggcta cagaacgagc catgttgcgg atacgggaaa
     2281 gataacctgc acgctccgtt acggatactg caccgcgggc atctaataaa ttgaatgtat
     2341 ggctgcattt caaaatataa tcatacgctg gatgaacaag attttcatcg atacagcgtt
     2401 tagcttcttt ttcaaaacga tcaaagttgc ttagtagcaa gtcttggtcg cttacttcaa
     2461 aagaatattt ggaatgttca tattctggtt gtttgaagat ctcgccatat ttcacgccat
     2521 cagcccattc taagtcatag acactttcaa cttcttgaat atatgaagca agacgttcta
     2581 gtccataagt aatctcagaa gtaactggtt tacaaggtag tcctccgact tgctggaagt
     2641 acgtgaattg tgtaatctcc atcccatcaa gccatacttc ccaacccaca ccggcacaac
     2701 ccatcgatgg gttctcccag ttgtcttcaa caaaacgaat atcgtgttct aatggatcaa
     2761 ttcctaaaaa acgcaagctt tctaaataaa gctcttggat attttcaggt gaaggtttca
     2821 ttacgacttg aaattgatga tgctgataca aacggtttgg gttttctcca taacgcccgt
     2881 cagctggtcg gcgggatggc tctacataag cagcattcca tggttccggt ccgattgccc
     2941 gtaagaatgt atatgggctc atcgtacctg ctcctttttc tgtatcataa gcttgcatca
     3001 gcatgcagcc gtttgatgac caaaattttt gtaatgttaa aatcatatct tggacggtca
     3061 attttttact catctgtttt gcccctttca atttttaagc gttgctgtca ataagaatac
     3121 aggaaagaaa acgacaaaaa gtccctatgt ctgattacag acatagggac gaataatttc
     3181 gcggttccac cctacttccg aataaagatt cggcagcttc atttatcgta ctcgcaagtg
     3241 cctcttcaaa aatcgtttgg gtccggcttc cactctcccg aactcgctaa accacgtgtt
     3301 ttttactgat cttggtcaat gtacgtattc attttgcagt tttcttgttt atataataga
     3361 tcctctcagt gaacgatcta tttaatctac ggttaataat agacggaagt ccctgtattt
     3421 gtcaaatagt tttgattttt ttgttgtatc tttcatttat tcttcttttt caggtatctc
     3481 tttttcaggc atatttttct ctggcatctt taatacgtgt gcccaagact tcatttgatc
     3541 aatgaacttt ttgcttttta gatgaatacc gacatattca tcatacaact ggtcaatgag
     3601 gtcacggatt gcctgcttcg tttcttcttt caagtggatt ccactgatct tatcataaga
     3661 aatagccgaa aacattcgaa cgaaataaac agcgcgagga tatgcatgat aacggtgttc
     3721 gtccattggc cagtgtttct cgcatatcac gccgccatat ttggaagaat agtcaaattt
     3781 tccttttgtt tccccgcata cgacacagtg cgtccaaatt ggcgcgattc cgaagcgttg
     3841 cagtatttga acttcaaaga tattcgtgat gatttctgca tcatctcctt ggtccatccg
     3901 ctgcagagct tgttgcgtaa attggaataa gtgcgggtca tattgatgat cttcgattgc
     3961 tgcatctact agatttagca aatacgtacc ataaccgctc aaaaagatat cttcttggat
     4021 gttccgaaac gcatggacgt ctttgacact gcttaaaaac gacaaacctt cacttagaaa
     4081 attaccgata tagactgctt cggtaaaggg gagcaaagcc ggagctaatg gctgattttg
     4141 ccgatggatt cctttgatat aaaacatcat tttgccagaa gattccgtaa agattttgac
     4201 taatttatct ttttctttgt aattacggct agataaaatc aagcctttcg attctgcaag
     4261 ctggctcata ttctccctcc taaaaaataa atctggaaaa gcagattctg ccgatccaga
     4321 ttatttatgt tacgatcttt cgctgttttt ctcaacgtac ggatttttat ccagtttatt
     4381 tctataccta acctataaga agtccagaat atcagtactc gtcttttcgg tagccgaagt
     4441 cttgcagata gacttttttg tctcgccagt ctttttggac tttgacccaa agttctagaa
     4501 atactttgtc tcctaaaaga ttttcgatat cctttcttgc tttcgttcct acttgcttca
     4561 gcatttttcc acctttgccg ataatgattc ctttttggct atctctttca acgatgatcg
     4621 tggcctggat atggatttta tcattctcgt ttcgcttcat cgaatcaaca acgactgcga
     4681 cagaatgagg cacttcgtct cgtgttaaaa acaagacttt ctcacggatc aattcagaaa
     4741 cgatgaagta ttcaggatga tcagtcactt gatcatctgg aaaatattgt gggccctctg
     4801 gcatttcact gactaataca tccattaagc gttcgacatt gtttccttct gtagcagaga
     4861 tcggaatcac ttgtgcaaaa ggcatctgcg tcgaataatc ttcgatgatt cccagcaact
     4921 cgtctggatg gatcttatcg attttattga taatcagata aactggagaa tgcacgtttt
     4981 ttagacgttc aataataaaa tcgtctcccc ggccgcgttt ttgatcagca ctgatcataa
     5041 ataagactgc atccacttct ttcaacgcag tgtaagctgc ttctaccata aaatctccta
     5101 gacgatgctt tggtttatgg attcctggtg tatcgataaa aatcagctga gcttctggtg
     5161 tagtatatac tccttggatt ttatttctag ttgtctgtgc cttgtcactc ataatggcaa
     5221 ttttttgacc cacgattcgg ttcaataatg tggattttcc tacattcggg cggccgacga
     5281 ttgcgacaaa ccctgattta tgttctggca tatacgttct cctttttaat taaacaacaa
     5341 atgatatatt ttagggacaa ataaaatcaa cccaatgatg acagcaaaac aagcggtcac
     5401 taaaactgct ccagctgcca tatctttgat ttttttccca atcggatgaa aatgaaaatc
     5461 cgtaaacatg tccactacat tttcaaaaac agtattgatg atctctacta tccaaacgag
     5521 aaaaacagct aaaagcagcc ataaccactc tatccgatca atctggaaaa tcacgcccat
     5581 aaataaagca aggataccga acgcaacatg tttcctcata ttgcgttctt cctcaaaaac
     5641 ggttttgact ccttggacag caaattccac ggacatcagg aaatgtttgt ttttctctac
     5701 gtttttatct tttgagtcca taggcatcca agatctcttt ctgtaaacca aacatttctt
     5761 tctcatcttc tggtgtcata tggtcatacc cgttgatatg caagaatcca tgtaccgcta
     5821 aaaagccaag ttcccgatca tatgaatggc cgtaagcttc tgcttgttca gcagcacgtt
     5881 caatagagat catgatgtct cctagtacac gaggcaatgg attctctcca tcttcaaaaa
     5941 tcactgggat ttcgtcttcg ccttcttctt ccagagcaaa actgatgacg tctgtcggtg
     6001 catcttttcc acggtaatca cgattgatta cttggattgc cgcattgtcc atgaaagtaa
     6061 cagacatttc tgtttcctct ggcagcttta aataagatgc tgcaaattgc agcaagtcat
     6121 cgatttcttt cattttctct tctgagactt ggtttgtttc atccataaag gtaatatcca
     6181 ttatacatcg cttctttcta ttcttcttgt tcttccttta tcttctcaac ttctgttttc
     6241 attttaggat atttgatccg agaatggaag gtgctactta aaccgctgat caatgatttt
     6301 tccacttttc ggatttcttt caaggtcaat ccactatcgt ctaattgacc atcggaaata
     6361 cgctcttcga taagattatg gacaaaactt tcgatttttt cgctcgttgg atgatccatt
     6421 gcccgtactg cagcctcaca actgtcagca atattgacga tccctgcttc acgactttgc
     6481 ggttttggtc caggataacg gaattgctct tctgttactt ccggattgcg ttctttcgct
     6541 ttgatgtaga aataacgcat tagcgtggtt ccatgatgct gtttgcaaat atcgatcacc
     6601 atttgcggca tattataatc ttccaatatt ttggctccat ctgtcacatg tccaaagata
     6661 atatgtttac tgtcttctgg caataagaaa ttgtgcgggt tttccgctcc tgaaggcaag
     6721 ttctcaacga aaaagctagc atgtttgatc ttgccgatat catgataata gcaagctact
     6781 cgagttagta aagaacgtcc gccgatttct gctacagcgt tagcacttag attagctacc
     6841 atcatactat gatgatacgt acctggcgct tcttccagca actgtttcaa taaaggatga
     6901 tttggattgc tgagttcatt taatacaatt acactgtcat cattgaccat caattcaata
     6961 tatgggtgta gaccgatcgt cagcaagaat gagaagagtg caccagtgaa cccgcagaca
     7021 agcattaacc aagtcgttgc atcaccaaag ctcattcctt gatagacgat caaaatgaca
     7081 tccatcatca caggaaaaat aatgacccac atggcagcag ataatccttg ttctgagatc
     7141 cgtttacgtc tgacaatcgt tgccatgagc ccagagaaaa cataagacat caagataatc
     7201 gttagaaagt tcgtgccaat cgaatcataa aaaatgaaaa gtgcggatac tgcttggaaa
     7261 ataccagcca tgatccctgc tcttcgattc aagaaaaagc ttaaaacaag tggtgcaaaa
     7321 gcagcgggat aaaacagcgg gatataagta gcttgttccg tttggaataa ttggaaaaat
     7381 ttcatcagca ctatgctgac tgacatagct gtcacataga aaagaacgta ttttgttctt
     7441 tctcctgtat tttggaattg aagtgaatta taaatcagta cgccgatttg aagcaaaaca
     7501 gctaaaacca tggcaattag cggaaaaatc gaagttgttt gattagtcag tcctagtaat
     7561 tccaattttt tcatagctga agcatcaatt tgattacctt cacgaacaat gatttctcct
     7621 tggaagatca tcactggttg tacagcttct cgtgcggatt gtttcaattc ttctgtcttt
     7681 ttctcattca atacgtcatt cactacgata ccttgatcca ctaagtagcg aatcatttgt
     7741 tgttgtgctg gtgtgacatt caaatattgg atttgctctt ctgcttcttg tttaaaagct
     7801 tccaattcac tttcacgaac ttgtttgctc atctgttcgt caatgagctt cagactctca
     7861 tccctcacag tatgaatctc agtttcagtc atttcaaata tcgtcgaata aaaattgttc
     7921 ggtagttttt gataaaacac aacattttct gcattcacat tttcaaaatt tttcttcagc
     7981 gcggcgatcc gctcttctgt agtaggcttt gggatatttt catcttcctt tgccttatca
     8041 atcttttcgt cataagattt gttgatggca tcattcgtct tatcgatcaa ttcaaatagt
     8101 tgcttgatcc gattgttctg gtcatctgca agatcttttt ggtaagtgta ttctggagtg
     8161 actgcttctg ctgccagttt tcttttttgt tcggtttctt ccgtgttctc gatcgttttg
     8221 ttcgcacgga tactttcttc tgctacttgg ccttctctga aatcattggc cttttgtttg
     8281 acactactaa atgtaacgcc gcagataatg ataaaaaaca agaacagcag gaaaggcaat
     8341 gctgcctttc caagtttttc ttgtagtttc tgcagtgttt tattcaacat gtgcttatcc
     8401 tcctaagcaa agcttattct tgatggagat ctgccttttc ataagctttg atgatttcgg
     8461 caactacagg gtgccgaaca acgtcacttg cttcaaaatt aacaaaatcg attttcttga
     8521 tttgctttaa tgttcgttct gcatgaacaa gaccacttgt tgtccctcga ggcagatcaa
     8581 tctgactcgt atctccattg acaatcattt ttgattgata accaagacgt gtcaggaaca
     8641 ttttcatctg agcaactgtc gtgttctgtg cttcatccaa aataacgaat gcatcatcca
     8701 atgtccgccc ccgcatgtaa gctaaaggag caatttcgat cacgcctcgt tccatcaatc
     8761 ggttcgtatg atctaatcca aaaatttggt ataaagcatc ataaacagga cgcagataag
     8821 gatctacttt ttcttttaga tccccgggaa gaaagcctag attttcccct gcttccacag
     8881 caggccgcgt caaaatgatt ttttgcactt cacctttttt caaggcagcg attgctaata
     8941 ctactgcaag aaaagttttt cctgtcccgg ctggtcctac gccaaatacg atatcatgtt
     9001 tggctaccga ttcgacatat ttcttttggc cgctgttttt tacccgtata ggttttccat
     9061 tacggtcttt gacgatctct tcctcataca tctcaacaaa ataatctaat gtgccttttt
     9121 gccccatttt cagtgccgtc acgacatctg gtgtacttac atggattcct cgtttgatca
     9181 attcctgcaa tgtccgaatg actgaggcag ccaaggaaat ctgggattgt tcgccaatga
     9241 tctgaatgat ttctccacgt gtatggatgg ttgtttctgt tgtttcttca atcagtttta
     9301 tgtgcttgtc gtgtgcacct aaaagcatgc tgacatcatc agcagccgtc aatctaatct
     9361 ctaaagaact agactcttcc gtcaaaaata gacatcccct ttttttcgat tcattctttc
     9421 ctattttacc atactcagaa gaaactatat agcaaataag aaaaggttct aaaagagttg
     9481 cttaaccata agtaccaaga aacgttaaaa aatagctgct tccattctct aaaggtctcg
     9541 tttgaataaa aaaagctgat catgtaagct atgcataaga tagaggacaa gtattccgtc
     9601 taacttagca agtattctct gggtattttt cttgtttcaa aaaaagaaaa caaaatattt
     9661 gaaatattct tgcatttcaa aaaaaacagg tgtattataa cgtcaacaaa taaatataca
     9721 gcaaagcgtc gaggaaaaca gtaggctatt cagtgattta ttcccagtga gtcatgtaag
     9781 tgagaaatga caataaatcg aatagacgaa catcatttcc gagtcaatgt cgaaaacgta
     9841 gatcattgtg gctgcctcca ttatcaggcc agcggataac tttatcgaaa agcgtccgtg
     9901 aatgaagaag cttttttaag cgaaaaaagg tggtaccgtt ctagaatcta gagccctttt
     9961 cccaagtgga aaagggcttt ttttatattt taaggtggtg gttttcatgg aatctgatat
    10021 tagtggtatt tctagtttct gcaacgttta ttttgttact caaaatgata attattggag
    10081 gaaaaattta tggaagaaac aaaaacttat tcatggaagc aattattgat ggtcagttca
    10141 cttatcttcg ggatgttctt cgggtcaggt aatctgatct ttcctgtcca cttaggtcaa
    10201 atggctggat caaactggat cagcgctggt gtaggatttg ccatctcagg cgctcttttc
    10261 ccattattag ctatcttagc tgtagtagtg acaaaaagtg atggtctgta tgacttagct
    10321 aaacctgtcg gaaaatggta tgctgccttg ttcttgatcc ttgttcattt aacgatcgga
    10381 ccattctttg gtacaccgcg gacagcagca actgcttttg aaatggctgc taaacccttt
    10441 ttgcctgaaa aatatgattc attaggtatg tttttattct ctgctctttt ctttttactt
    10501 gcttatttcc ttactgtaca tccaacacgt ttggtcaaat atgtcggaaa gtatttaaac
    10561 tccttgtttc ttgcattatt agctatcatt tttgtcgttt ctttattcca acccatgggg
    10621 aatctaaacc aatctgctgc agattcttat cagacgaacg ctttgacaaa tggcgtttta
    10681 gaaggataca acacggttga tgcagtagct ttacttgctt taagtgtcac ttttgtccat
    10741 gcagttcgtg atcttggttt taaagataaa aaagtcacag aattaacagc aaaagctggt
    10801 agcctgagcg ttgcgttaga agtcatgatt tattttggtc tggtccttct cggtgcgatg
    10861 agtttgaata agatgtcttt atcagaaaat ggcggaactg cactttctca gatcactaat
    10921 ttttatttag gtaacttcgg tgctagcttc ttaggggtaa tggtcacatt aggtgtcttc
    10981 actaccgcta tgggactcgt tgtttctttt gcacaagatt tccataaact gttccctaaa
    11041 gtcagttata tgacatggct gcggttaacg acttttgtct cttttgtcgt tgcgaatgcc
    11101 ggattggata atatcatcca atggtcttta ccagtattga tgcttttgta tccattgtca
    11161 cttgcgttaa tcttgctttc cttaacagca aaattcttcc aaaagagtcc atttgtctat
    11221 caagtaacga tgttcttttc tgccgttcca gccgtattgg atatgttggc aaattcgcct
    11281 gcattagtga gtcagcaaac tgtcgtagca agcttactag gattttatca tcattatgtt
    11341 ccttttgctg cattaggact tggctgggtc gtcccaacgt tgcttggtta cgctggcagc
    11401 ttgctctttt attatgtata tcgcgtatca ggatataaac aagaagcaag tgagttacca
    11461 gaagaataac caaatcaaac acaatttaac ataagagata ttaaacagga
//
LOCUS       DACCQI010000091        11469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCQI010000091 DACCQI010000000
VERSION     DACCQI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.095"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            53..169
                     /locus_tag="IVO76_002102"
     CDS             53..169
                     /locus_tag="IVO76_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7430046.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            185..2104
                     /gene="tet(M)"
                     /locus_tag="IVO76_002103"
     CDS             185..2104
                     /gene="tet(M)"
                     /locus_tag="IVO76_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7430047.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVTCALLQEKYHVEVKIKKPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYDDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRNDLTFFTNGRSVCLTELKGYQVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2219..2269
                     /locus_tag="IVO76_002104"
                     /pseudo
     CDS             2219..2269
                     /locus_tag="IVO76_002104"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2303..3679
                     /gene="tet(L)"
                     /locus_tag="IVO76_002105"
     CDS             2303..3679
                     /gene="tet(L)"
                     /locus_tag="IVO76_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7430048.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTALYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FPILILARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTAAFFLEVTPWLLTIILVFVFGGLSFTKTVISTIVSSSLKQKEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSVRLLNQKLLPMDLDQSTYLYSNMLFLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            4243..5505
                     /locus_tag="IVO76_002106"
     CDS             4243..5505
                     /locus_tag="IVO76_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP7430049.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5750..6289
                     /locus_tag="IVO76_002107"
     CDS             5750..6289
                     /locus_tag="IVO76_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP7430050.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHS"
     gene            complement(6282..7253)
                     /locus_tag="IVO76_002108"
                     /pseudo
     CDS             complement(6282..7253)
                     /locus_tag="IVO76_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            complement(7444..8763)
                     /locus_tag="IVO76_002109"
     CDS             complement(7444..8763)
                     /locus_tag="IVO76_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
                     /protein_id="HAP7430051.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGRLSSDSGLVLVKELMNTFGFSH
                     LAKQWLHITDHRAYFTHDNLAILEQLIMQLIAGYSADSSANLLRRDPVFQAVLGKKQL
                     ASQSSISRFLDRFTEENIDQLQALNQSLIDKARLIRNDTELIIDVDSTHSDTFGRQEQ
                     TDYNAHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKAFINPLLHHYKSTLPH
                     TEILVRGDSGFATPEVYESCEATESQYVIRLKSNRRLSQLAEHSVLYGDNQEWEEREV
                     QYFSLPYQAQSWSKPRRVCIRSVREGGELLFHHAFIVTNLSDNVSPEVIFSLYGKRGT
                     MENFIKEAKGGFYFDKTDSPRFLENHVRMMISVLAYNLVNFLKSIGFDKVNQGMTIHS
                     IRLILLKVAGKLVQTGRQVYLKLSSYHVYQTEFYKVFERLRRSKQWI"
     gene            complement(8991..9518)
                     /locus_tag="IVO76_002110"
     CDS             complement(8991..9518)
                     /locus_tag="IVO76_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7430052.1"
                     /translation="MESTDVINEIRGILPRNSIGKYDLLTIFTHKTVFDKIVKVLSLD
                     FQNKVDYVAAPEAIGWILGTAIAKELGVGFIGVRKGDKLPYAKEEIISTHFTDYSGQD
                     KSFEISKSSIVRNKRVLIVDDWIETGSQMKALIALLEKLDCSIIGLATIGIDINEVTQ
                     KWIDSSFVAYIGSNI"
     gene            complement(9562..10425)
                     /locus_tag="IVO76_002111"
     CDS             complement(9562..10425)
                     /locus_tag="IVO76_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7430053.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHLNQILRFELLRMMSWKVGIKTEFSLSVGKNYKYINKYID
                     EDLWNRLLSTYRMDSYENIWKSLFICHQLFREVSKEVAELLGFDYPEYGKNITRYTED
                     MYKKYVENDYF"
     gene            complement(10458..11192)
                     /locus_tag="IVO76_002112"
     CDS             complement(10458..11192)
                     /locus_tag="IVO76_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7430054.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            complement(11173..>11469)
                     /locus_tag="IVO76_002113"
     CDS             complement(11173..>11469)
                     /locus_tag="IVO76_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7430055.1"
                     /translation="KYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKP
                     EKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI"
BASE COUNT         3655 a         1960 c         2123 g         3731 t
ORIGIN      
        1 ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct
       61 aaagtatcca gagaatatct gtatgctttg tatacctatg gttatgcata aaaatcccag
      121 tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa tggaggaaaa
      181 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt
      241 aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg
      301 tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg
      361 aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat
      421 ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat
      481 ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat
      541 ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac
      601 ggtttatcag gatattaaag agaaactttc tatggaaatt ataatcaaac agaaagtaga
      661 gctgcaccct aatatgtgtg tgatgagctg tacggaacct gagcaatggg atgtggtaat
      721 agaaggaaat gatgaccttt tagagaaata tatgtccggt aaatcattag aagcattaga
      781 actcgaacaa gaggaaatca gaagatttca gaattgctcc ttgtaccctg tttatcatgg
      841 aagcgcaaaa agcaacatag ggattgagca gcttatagaa gtgataacga ataaatttta
      901 ttcatcaaca tacagaaaga agtctgaact ttgcggaaat gtcttcaaaa ttgaatattc
      961 ggaagaaaga caacgtcttg catatgtacg cctttatggc ggaatcctgc atttgcggga
     1021 ttcggttaga atatcggaaa aggaaaaaat aaaaattaca gaaatgtata cttcaataaa
     1081 tggtgaatta tgtaaaattg ataaggctta ttccggggaa attgttattt tgcaaaatga
     1141 gtttttgaag ctaaatagtg ttcttggaga tacaaagcta ttgccacaga gagagagaat
     1201 tgaaaatccg ctccctctgc tgcaaacaac tgttgaaccg agcaaacctc aacaaaggga
     1261 aatgttactt gatgcacttt tagaaatctc cgacagtgac ccgcttctac aatattatgt
     1321 ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa tggaagtgac
     1381 ttgtgctcta ttgcaagaaa agtatcatgt ggaggtaaaa ataaaaaagc ctacagtcat
     1441 ttatatggaa agaccgttaa aaaaagcaga gtataccatt cacatcgaag tgccaccgaa
     1501 tcccttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa gtggaatgca
     1561 gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg cagttatgga
     1621 agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg attgtaaaat
     1681 ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt ttcggatgct
     1741 tgctcctatt gtattggaac aagttttaaa aaaagctgga acagaattgt tagagccata
     1801 tcttagtttt aaaatttatg caccacaaga atatctttca cgagcatatg acgatgctcc
     1861 taaatattgt gcgaacatcg tagacactca actgaaaaat aatgaggtca ttcttagtgg
     1921 agaaattcct gctcggtgta ttcaagaata tcgtaatgat ttaactttct ttacaaatgg
     1981 acgtagcgtt tgtttaacag agttaaaagg atatcaggtt accactggcg aacctgtttg
     2041 ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca ataaaataac
     2101 ttagcacatt ttatattgtt atataaatgt ggcttcttgt taaacgaaac gagatgaaat
     2161 attttttaat aaagatttga attaaagtgt aaaggaggaa ttaattatga agtaaaatga
     2221 atgtaacagg gttcaattaa aagagggaag cgtatcatta accctataaa ctacgtctgc
     2281 cctcattatt ggagggtgaa atgtgaatac atcctattca caatcgaatt tacgacacaa
     2341 ccaaatttta atttggcttt gcattttatc tttttttagc gtattaaatg aaatggtttt
     2401 gaacgtctca ttacctgata ttgcaaatga ttttaataaa ccaccggcga gtacaaactg
     2461 ggtgaacaca gcctttatgt taaccttttc tattggaaca gctctatatg gaaagctatc
     2521 tgatcagcta ggcattaaaa ggttactcct atttgggatt ataataaatt gcttcggatc
     2581 ggtaattggg tttgttggac attctttctt tcccatactt attctggctc gatttattca
     2641 aggagctggt gcagctgcat ttccagcact cgtgatggtt gtagttgcgc gctatattcc
     2701 aaaggaaaat aggggtaaag catttggtct tattggatcg atagtagcca tgggagaagg
     2761 agtcggtcca gcgattggtg gaatgatagc ccattatatt cattggtcct atcttctact
     2821 cattcctatg ataacaatta tcactgttcc gtttcttatg aaattattaa agaaagaagt
     2881 aaggataaaa ggtcattttg atatcaaagg aattatacta atgtctgtag gcattgtatt
     2941 ttttatgttg tttacaacat catatagcat ttcttttctt atcgttagcg tgctgtcatt
     3001 cctgatattt gtaaaacata tcaggaaagt aacagatcct tttgttgatc ccggattagg
     3061 gaaaaatata ccttttatga ttggagttct ttgtggggga attatatttg gaacagtagc
     3121 agggtttgtc tctatggttc cttatatgat gaaagatgtt caccagctaa gtactgccga
     3181 aatcggaagt gtaattattt tccctggaac aatgagtgtc attattttcg gctacattgg
     3241 tgggatactt gttgatagaa gaggtccttt atacgtgtta aacatcggag ttacatttct
     3301 ttctgttagc tttttaactg ctgccttttt tttagaagta acaccatggt tactgacaat
     3361 tatattagtt tttgtttttg gtgggctgtc attcaccaaa acagttatat ctacaattgt
     3421 ttcaagtagt ttgaaacaaa aggaagctgg tgccggaatg agtttgctta attttaccag
     3481 ctttttatca gagggaacag gtattgcaat tgttggtggt ttattatctg tacgcttgct
     3541 gaatcagaag ttgctaccta tggaccttga tcaatccact tacttgtata gtaatatgct
     3601 attccttttt tcaggaatca ttgtcattag ttggctggtt accttgaatg tatataaaca
     3661 ttctcaaagg gatttctaaa tcgttaaggg atcaactttg ggagagagtt caaaattgat
     3721 ccttttttta taacaggaat tcaaatcttt ttgttccatt aaagggcgcg attgctgaat
     3781 aaaagatacg agagacctct cttgtatctt ttttattttg agtggttttg tccgttacac
     3841 tagaaaaccg aaagacaata aaaattttat tcttgctgag tctggctttc ggtaagctag
     3901 acaaaacgga caaaataaaa attggcaagg gtttaaaggt ggagattttt tgagtgatct
     3961 tctcaaaaaa tactacctgt cccttgctga tttttaaacg agcacgagag caaaaccccc
     4021 ctttgctgag gtggcagagg gcaggttttt ttgtttcttt tttctcgtaa aaaaaagaaa
     4081 ggtcttaaag gttttatggt tttggtcggc actgccgaca gcctcgcaga gcacacactt
     4141 tatgaatata aagtatagtg tgttatactt tacttggaag tggttgccgg aaagagcgaa
     4201 aatgcctcac atttgtgcca cctaaaaagg agcgatttac atatgagtta tgcagtttgt
     4261 agaatgcaaa aagtgaaatc agctggacta aaaggcatgc aatttcataa tcaaagagag
     4321 cgaaaaagta gaacgaatga tgatattgac catgagcgaa cacgtgaaaa ttatgatttg
     4381 aaaaatgata aaaatattga ttacaacgaa cgtgtcaaag aaattattga atcacaaaaa
     4441 acaggtacaa gaaaaacgag gaaagatgct gttcttgtaa atgagttgct agtaacatct
     4501 gaccgagatt tttttgagca actggatcca ggagaacaaa aacgattttt tgaggaaagt
     4561 tataaattat tttccgaacg atatggcaag caaaatattg cttatgcaac agttcataat
     4621 gatgagcaaa cccctcacat gcatttaggt gttgtgccta tgcgtgatgg aaaactgcaa
     4681 ggaaaaaatg tgtttaatcg tcaagaactg ttatggctac aagataaatt ccccgagcat
     4741 atgaaaaaac agggttttga gttgaagcgt ggtgaacgtg gctctgaccg taaacatatt
     4801 gagacagcta aatttaaaaa acaaactttg gaaaaagaga ttgattttct agaaaaaaat
     4861 ttagcagtta aaaaagatga atggactgct tatagcgata aagttaaatc agatttagaa
     4921 gtaccagcga aacgacacat gaaaagtgtt gaagtgccaa cgggtgaaaa gtccatgttt
     4981 ggtttgggaa aagaaataat gaaaacagaa aagaaaccaa ccaaaaatgt tgttatatcg
     5041 gagcgtgatt ataaaaactt agtgactgct gcgagagata acgataggtt aaaacagcat
     5101 gttagaaatc tcatgagtac tgatatggcg agagaatata aaaaattaag taaagaacat
     5161 gggcaagtta aagaaaaata tagtggtctt gtagagcgat ttaatgaaaa tgtaaatgat
     5221 tataatgagt tgcttgaaga aaacaagtct ttaaagtcta aaataagcga tttaaagcgt
     5281 gatgtgagtt taatctatga aagcactaag gaattcctta aggaacgtac agacggctta
     5341 aaagccttta aaaacgtttt taaggggttt gtagacaagg taaaggataa aacagcacaa
     5401 ttccaagaaa aacacgattt agaacctaaa aagaacgaat ttgaactaac tcataaccga
     5461 gaggtaaaaa aagaacgaag tcgagatcag ggaatgagtt tataaaataa aaaaagcacc
     5521 tgaaaaggtg tctttttttg atggttttga acttgttctt tcttatcttg atacatatag
     5581 aaataacgtc atttttattt tagttgctga aaggtgcgtt gaagtgttgg tatgtatgtg
     5641 ttttaaagta ttgaaaaccc ttaaaattgg ttgcacagaa aaaccccatc tgttaaagtt
     5701 ataagtgacc aaacaaataa ctaaatagat gggggtttct tttaatatta tgtgtcctaa
     5761 tagtagcatt tattcagatg aaaaatcaag ggttttagtg gacaagacaa aaagtggaaa
     5821 agtgagacca tggagagaaa agaaaatcgc taatgttgat tactttgaac ttctgcatat
     5881 tcttgaattt aaaaaggctg aaagagtaaa agattgtgct gaaatattag agtataaaca
     5941 aaatcgtgaa acaggcgaaa gaaagttgta tcgagtgtgg ttttgtaaat ccaggctttg
     6001 tccaatgtgc aactggagga gagcaatgaa acatggcatt cagtcacaaa aggttgttgc
     6061 tgaagttatt aaacaaaagc caacagttcg ttggttgttt ctcacattaa cagttaaaaa
     6121 tgtttatgat ggcgaagaat taaataagag tttgtcagat atggctcaag gatttcgccg
     6181 aatgatgcaa tataaaaaaa ttaataaaaa tcttgttggt tttatgcgtg caacggaagt
     6241 gacaataaat aataaagata attcttataa tcagcacatg cattcttaat cacttggata
     6301 tattcttttt gttttaacgc ttcaataata cttgctcttg tcgcttctgt tccgatcccc
     6361 tcgacttctt gtaaaatctt gattgcttct tcatcatcaa ccgttttatt agcggttttc
     6421 atagcagtta ataatgtacc ctctgtaaaa gctttcggtg gttgtgtttc tttttcggca
     6481 ctcttaacgt caacctcggc atgttctcca atgataacca agggtaacgt ttgaacgtcc
     6541 tctttttctt ctttggtttg ttgtttgaaa agaattttcc aaccttcttg ttttggtgtc
     6601 tttccgatcg attgaaacag caaatcagct actttcgttt gtatcttggt ttcttcatac
     6661 aagtaatcag gtaaaaacat ggcaacggtc gttttaacga ctaaagcata tatttttcgt
     6721 tgtaaatcgt ccatttttgc taatgcagat tcggtaggta cttgttttgt tgggataatg
     6781 gcgtggtgtt cctgtacctt actaccgtcc acataacgct ttctaggctc tgtttgaacc
     6841 atttcgagat caagtcctaa aaaaccgcta tatttgccaa aatttgcttt taaataagca
     6901 aattcgttct ctgtaataaa tggtgtatct gttcttggat aactcaataa ttttgcttca
     6961 tacagtcctt gcatagcttt taaagtttgg cttgctgtcg ctttataaag ctgattgact
     7021 tttgattgta aactacttaa agaaaacaaa ctcggactat tcgttttctt ctctttggtt
     7081 tgaacatcag caattatccc ctcttgattg cctattttag cttgtttaga agaaacaaag
     7141 gataaaagtt cctcttggct cttaaaacgc tgtgtggggc ttagaacgcc cttaaacgac
     7201 ccttggttta cttttatact agcttccacc tcgaaaaaac ctgaataatt catagctcta
     7261 aatttaacct gtttttttga acaaattgcc aatttttaaa ttaggcagat actttcttcc
     7321 aaaataatgt gttttcgaac aggatgccta cggaagaaga tcataataaa aattatttgt
     7381 ctatttaagg gcagacgaat cccttgaaaa agaatcggtt aaaaaatttg tgtgataact
     7441 agtctaaatc cattgtttgg atcgccgcag gcgttcaaaa accttataaa attcagtttg
     7501 atacacatga taacttgaca gtttgagata gacctgtctc ccagtctgaa caagtttacc
     7561 ggctacttta agcaatatca gccggatgga atgaatcgtc atgccctggt tcactttatc
     7621 aaaaccaatg ctttttaaaa aattaacgag gttgtatgca aggacactga tcatcattct
     7681 gacatgattt tctaagaaac gcgggctatc tgttttatcg aagtaaaagc cacctttcgc
     7741 ttctttgatg aaattttcca ttgttcctcg cttgccgtaa agagaaaata tgacttcagg
     7801 cgagacattg tcggatagat tagtcacaat aaacgcatga tgaaatagca attctcctcc
     7861 ttcacggacg gagcgtatac agacacgacg aggtttcgac caggattgtg cttgataagg
     7921 tagtgaaaaa tactgcactt ctcgctcttc ccattcttga ttgtccccgt aaagaacaga
     7981 atgttcagct aactgactta acctccgatt gctctttaat cggataacgt actggctttc
     8041 agttgcttca caagactcat acacctctgg tgtggcgaag ccgctgtcac cccgaaccaa
     8101 tatctcggta tgaggtaacg tgctcttgta gtggtgtaaa agcgggttga taaaggcttt
     8161 taccccttta gatgtgtact gattgcctga acgcagttca gcttttaaga aatctccggt
     8221 cagtccatca aaggcaacta atgggtgata gccgtaggtt tgataatggg cattataatc
     8281 tgtttgttct tggcgtccaa aggtatccga atgtgtggaa tcgacatcaa tgattaactc
     8341 ggtgtcattg cgaattaaac gtgctttatc aatcaatgac tgatttaatg cttggagttg
     8401 atcgatgttc tcctcggtga aacgatccag aaaccgtgag attgaggatt gtgatgccaa
     8461 ctgtttttta ccgagtacag cttgaaagac aggatcccgt cttaatagat tagctgatga
     8521 gtctgccgag taaccagcaa ttaattgcat aataagctgt tccaatatcg ctaagttatc
     8581 atgggtaaaa taagcccgat gatctgtaat atggagccat tgtttagcta agtgcgaaaa
     8641 gccgaaggta ttcattaatt ctttaaccag gactaacccc gaatcacttg atagacgacc
     8701 accggtatgt gaaatagtga tatttgaatt gaatttaact tgatttttgt gtaagctagt
     8761 catgagaaga actcctttct tatggttggt ttcgtcacct ttaccatagc agaatggggt
     8821 tcttttttca tcacttaagg ggtgaaacta aaaaagtaat tatcagactg ccgttaggca
     8881 gtttcagacg gtttcaatta aaaagtatga attattcagg gtaaacatct ttcgttgtat
     8941 tgtaaattaa cgccatccaa aattttgatt caagattttc tttcggttta ttatatgttt
     9001 gagccaatat aagctacaaa actgctgtct atccattttt gagtaacctc atttatatca
     9061 atcccaatag tagccaaccc aataatgctg caatccaatt tctctaacaa agctattaaa
     9121 gccttcattt gagaacctgt ctctatccaa tcatctacaa tcaaaactct tttgttcctc
     9181 acaatagaac tcttagatat ttcaaagctt ttatcttgac ctgaatagtc ggtaaaatgt
     9241 gtcgaaataa tctcttcttt tgcgtatggc agcttatcac cttttcttac tccaataaac
     9301 cccactccaa gttctttggc tatggcagtg cctagtatcc atccaattgc ttctggggca
     9361 gccacataat ctactttatt ttgaaaatcc aatgatagta cttttacaat tttatcaaaa
     9421 acggttttat gtgtaaaaat agttagtaaa tcatattttc caattgagtt acgaggtaat
     9481 atacctctaa tttcatttat aacatcagta ctttccatac atttacaccc cttgcttatg
     9541 gttaataatc tttgctaatc tttaaaaata gtcattttca acatattttt tatacatgtc
     9601 ctcggtatat cttgttatgt tcttaccata ctctggataa tcaaacccca gtagttctgc
     9661 tacctctttg gacacttccc tgaacaattg gtggcatata aataatgact tccaaatatt
     9721 ttcataggaa tccatgcgat atgtagataa taatctattc catagatctt catcaatgta
     9781 tttgttaata tacttataat ttttcccaac acttaatgaa aattctgtct ttatcccaac
     9841 cttccacgac atcatcctaa gtagttcaaa ccgtagaatc tggttcagat gatcgattgc
     9901 aaacagtatc tctttgcggc acaatccttt aataacataa ggtgttacat tccaaaattc
     9961 attgcagcaa tcatcatact cccttgcgct tggctttctt acatgataat ctatatcagt
    10021 cggaactatg tcccttttaa ttctacaatc tttatcaatt agaaccttta ttaatttatc
    10081 gccctttagg taattatcta actcttccaa gggcaataag gtaagatcaa ttttattgta
    10141 atcatcaaat agcataagat aggaaaatcc cttttcttca ggtgggaata attccatatc
    10201 ctccggcttt tgcatcatta ttatattccc aaattgatta agccagtcat cattagatat
    10261 aaacggttct atatcactta caaaatatgt aatatcataa tcctgaaatt catctttagg
    10321 tatattaata tttgcgcgtg acccctcaag ggtcacaatt cgaatacgtt catcctgttc
    10381 tgctaaagaa agtactaaat ccatcatttc tttttctgat ctcatttaca tactcctcgt
    10441 ttattttttc tatattatta catcttcttt tttgccgata caatcagcat cattgggcgt
    10501 cgcatttcat ccgccatccc cggaatatcc atcatgttct ctggcggctg tggctccaca
    10561 atctgattta ttataaaact atttgaaagc agtgtattta gatatgtggt cagtgttcta
    10621 tgatattttg taaccttttc ttccaaaaac atagctgtcc gtttgccctc ataataataa
    10681 ttgtccaccg ggaaatgcag tatttctcct ttttcgttat aataccagtc ttgtgttcca
    10741 tgagcagtaa aaacaggatg ttcaactgta aaaactaaat tgccaccagc cttcagcatc
    10801 ctatatatct tttttattaa attctcatag tctgctacat aatgaaacgc aagcgaactt
    10861 agtattacat caaagctctc ctctgggaaa tccacatctt ctatggcaca gcattcatat
    10921 tcaatctgtg gaaaatgggt ttttcctttt gctacttcga gcattttatg agaaatatca
    10981 acacctacta cagaggaagc accgttttcc atcgcatata tacagtgcca tccatagccg
    11041 catcctaaat caagcacacg cttaccctta aaatcaggta gcatcttttt caaagtctcc
    11101 cattctcccg caccagccag tcctttctgc gagcgactca tttgactgta tttttgaaaa
    11161 aatatattat catcatattt gttttctttc atctgaactc tcctcgttta aaaagttatt
    11221 tatctccgat acaatttcat tcacttcttt ataaagtttc tcggtcatat cgtagcattc
    11281 aaaaagagaa agaccgagta cttcaaaaat atgattaact ctttcggcat atttttcagg
    11341 tttatattca aaagtttcaa gcagttttat agctttcttt tcgttaatgc aataagcatt
    11401 attacatgca aatagtactt gatttaagca tgaaataata cgaaaaacat ggcctgcaat
    11461 ataatattt
//
LOCUS       DACCQI010000092        11461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCQI010000092 DACCQI010000000
VERSION     DACCQI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.096"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(98..1393)
                     /gene="purB"
                     /locus_tag="IVO76_002114"
     CDS             complement(98..1393)
                     /gene="purB"
                     /locus_tag="IVO76_002114"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7430056.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1406..2539)
                     /gene="purK"
                     /locus_tag="IVO76_002115"
     CDS             complement(1406..2539)
                     /gene="purK"
                     /locus_tag="IVO76_002115"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7430057.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMGQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2523..3011)
                     /gene="purE"
                     /locus_tag="IVO76_002116"
     CDS             complement(2523..3011)
                     /gene="purE"
                     /locus_tag="IVO76_002116"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7430058.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3427..4734)
                     /locus_tag="IVO76_002117"
     CDS             complement(3427..4734)
                     /locus_tag="IVO76_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7430059.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4737..5318)
                     /locus_tag="IVO76_002118"
     CDS             complement(4737..5318)
                     /locus_tag="IVO76_002118"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7430060.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5434..5531)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5890..7182)
                     /locus_tag="IVO76_002119"
     CDS             complement(5890..7182)
                     /locus_tag="IVO76_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7430061.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7179..7802)
                     /locus_tag="IVO76_002120"
     CDS             complement(7179..7802)
                     /locus_tag="IVO76_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430062.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7805..8641)
                     /locus_tag="IVO76_002121"
     CDS             complement(7805..8641)
                     /locus_tag="IVO76_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7430063.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8658..9545)
                     /locus_tag="IVO76_002122"
     CDS             complement(8658..9545)
                     /locus_tag="IVO76_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7430064.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9561..10844)
                     /locus_tag="IVO76_002123"
     CDS             complement(9561..10844)
                     /locus_tag="IVO76_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7430065.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
BASE COUNT         3461 a         2570 c         1915 g         3515 t
ORIGIN      
        1 ataataaact aaaagaagca gaaccgctaa cggatctgct tcttttttgg ttatttttgc
       61 tattctattt taattgaatt ttgcaactca ctcttcttta ttccaaacct actcgtttga
      121 atatcacgtc aacgtttttc aaatgatgat gataatcaaa ggcatcgtct atttcttctt
      181 tagataatac cgatgtgatg gttggatctt cttctaacag cgggcggaac tgcacttggt
      241 tgtcccaagc gtacgcagtt tttggctgaa caagatcata agcttcttct cttgtcattc
      301 ccttatcgat caatttcagc ataacgcgct ggctataaat caaaccaaat gttgcagaca
      361 tattccgctt catgttttca ggaaagaccg ttagattttt aacgatattg ccaaaacgat
      421 ttaacatgta attcaataaa atcgtggtat caggcaagat gattcgttct gctgaagaat
      481 gagaaatatc tctttcatgc cataatgtga cattttcata ggcagtgacc atgtgtccgc
      541 gcaccactct ggctagacca gccatatttt ctgaaccgat cggattcctt ttgtgcggca
      601 tagcagaaga acctttttga cctttcgcaa aaaattcttc tacttcacgt gtttcggatt
      661 tttgcaatcc gcgtatctct gttgcaaaac gttcaatact tgtagcaata agcgccatcg
      721 ctgagaaata ttcagcatgt agatcccgag ggagaacttg agtagagatt tcttgtggac
      781 ggatccccaa ttgtttacat acatactctt caataaatgg cgggatattt gcgaacgttc
      841 ccactgctcc tgagattttt cctgcttcta ctccttttgc cgcatgttca aatcgttcga
      901 tgttccgttt catttctgaa taccatgtgg caagtttcaa tccaaaagta gttggttcag
      961 catggacacc gtgtgtccga cccatcatca ctgtatattt gtgttctttt gctttttcgc
     1021 caatgatttc cgtaaaacgt ttcaagtctt cccgtaaaat atcatttgct tgcttgatga
     1081 gatagccata agcagtatct acgacatccg tacttgttag accataatgg acccattttc
     1141 tttcttctcc caatgtttca gaaaccgctc gtgtaaacgc aacgacatca tggcgtgtta
     1201 atttttcgat ttccaagatc cgatcgatat caaatgatgc atgctcacga atttttttca
     1261 catcttcttg cgggatttca ccaagttctg cccaagcttc atctgctaaa atctcgactt
     1321 cgagccacgc acggtatttg ttctcttccg tccagatatt ccccatttca gggcgtgtat
     1381 aacgttctaa catgtttttt ctcctctaat cccaaatatt cgtttgtttg atttcttcca
     1441 gcgtttgctg gatgtcttct gtcaagatag tgatatgtcc catcttgcga cctttttttg
     1501 cttgtttttt tccatagaaa tggaattgcc agtctggctt attctcaatc aagcgatagc
     1561 tttccagcag ttgttctccc aaaacattga ccattactgc attgcttatc aaacgtactt
     1621 tttctcctaa tggccaacca caaatacctc gaatatgggc atcgaactga cccatagaac
     1681 aagcttcaat agaataatgt ccagaattgt gaggacgagg agctaattca ttcacatata
     1741 gcccgcctgt ttttgttaaa aacatttcta cccctaaaac acctgaaagg ctcactgctt
     1801 ctgcaatcac acgggcaata cgctcagcct cctcgatcac atcctgatca atcgcggctg
     1861 gagcaatcgt ttcatgaaga atattgttgt ggtggatgtt ttccacgacc ggaaaagtcg
     1921 tgaaatctcc ttgaccattt cctgccacca tgatcgaaat ctctttttca aacgggatcc
     1981 atgcttctaa aacacaggtt ccttctctta aaaggtccat ggcaggagct agatccgccc
     2041 gacttttcaa aacatactgc cccttgccat catagccgcc tcgagtggtt ttcaaaacac
     2101 aaggatagcc gatcccgtca atcgcatctt ggatatccgt cggactgacg atcgttgcat
     2161 aaggagcaat cacgatgtta ttcgtttcca aaaaagattt ttctaacagg cggtcttgcg
     2221 taatcgcaag caagtctgtc ccttgaggaa taaatgacat gggtaaaatc gcatttaacg
     2281 cctctacact gacgttttcg aattcatagg taatgacatc tgttcttctg gcaagctctt
     2341 tcagcgcaaa ggtgtcatca tagacacttt caatgtgcca atctgcaact tgtgcggctg
     2401 gacaatcgct cacaggatcc agcacaccta ctttaaagcc catttctttt gcactaatcg
     2461 ccatcatacg acctaattga ccgccaccga cgatcccgat cgttgaacca ggcaacaaag
     2521 gtttaaccaa gttgatcact actttctata acagttttag ccaacattga acgtctttca
     2581 gccaatttag cctccaattc taaatcatac atggaaagca tctgtatcgc aagtagtcca
     2641 gcattaattg ctcccgcttt tccgattgct gtagtagcta caggtactcc tccaggcatt
     2701 tggacaatcg aaagaagcga atccaaacca tttaatgttc gtgattggac cggtacaccg
     2761 ataaccggca aagttgtttt tgatgcgacc atacccggca gatgcgcggc tccccctgct
     2821 cctgcaataa tgattttgat tccttttttt cgagcgtttt gtgcatattc aaacatcaaa
     2881 tctggggttc gatgagcaga aacgaccttt ttttcataag acacaccgaa ttcatccaat
     2941 atttgacatg cttctttcat tgtttcccaa tctgaagtac tccccataat cacagagatt
     3001 tttgctgtca ttgtttttcc tcccagtttt tctacattca tattttaaca atcacttttt
     3061 ttgatgtcaa agaaatttcg aatgttaaca aagaaaaaca ttttattgtt cgtgtttatt
     3121 aaagataaaa aaaaggaatg tgacaaaagt taggtcacat tcctttttat ggataaacgg
     3181 tgttctcctg ccaactcctc ggtcacaagg aacaatattc aacaaacatg agaagctttt
     3241 ttttgaaatt gttccttgtg actcggagat gaacggcagg ctcacgacct cttccttcaa
     3301 cggtgttcaa tcagctgacc ctcggtatta gctcaaacaa acggcaaaat acgaggaact
     3361 atttttccgt ttgtttctct aacaatcagg tcaaaacgct gatttcacaa cctttctttt
     3421 gctctattac ttcttgaagg cttcttccaa tatttcttca tcttttttca atccattaaa
     3481 gatcaaattc aatatgatag ccgtaatgct actcattacg atgccatttc cagtgaacat
     3541 ttgcaatgtt tgcggcattt gactgaataa tgtcggcgta acattgaaac ccagcccaaa
     3601 tccaacagaa acagcaacaa tcaatagatt tttatcattg ctgaaatcaa cttccaacag
     3661 catacggatt ccttggactg ccaccatccc gaacatgact aacattcctc caccaagtac
     3721 cggctcaggg atgatttgtg ctaacgcacc gattttaggg aataatccta aaacaatcaa
     3781 gaagaatgca gagaaataga tgggttttct tgttttgatt cccgaaagtt gaaccaatcc
     3841 gacattttga gaaaagccag tataagggaa tgtattgaaa ataccgccta agataacggc
     3901 aagtccttct gcacgatatc cctttttcaa ttcttcttct ccaacatttt tcccagtaat
     3961 gtcaccaagt gcaaaatata cacctgttga ttctaccatg ctgaccatgg aaatgatgat
     4021 cattagtaaa atcgaccata cgtcgaaagt tggtttacca aaataaaagg gttgtggaac
     4081 atggaacaac ggcgcatctc caatcgcgct cagatcaatc attcccatga aagcagctaa
     4141 gatgcttcct ccgactaaac caatcaatac cgcgattgaa cgaatgaacc cttttgccag
     4201 catttgcacg cctacaatta ggagaatcgt cacaaatgca agtagcagat ttgttttgtc
     4261 accaaaatca gctgctgttg cattacctcc acccattttc tcgattgcta caggaatcaa
     4321 agtcaaacca ataacggtga taaccgttcc tgttactata ggagggaata gttttttaat
     4381 tttagaaaag acaccagaaa ctaacacaac aaagatccca gaagcgataa tagatccgta
     4441 gatagctcct actccttcat tggttccgat caagatcagt ggagctactg cttggatcgc
     4501 acatcctaaa acgacaggca agccaattcc gaaaaatcgg ttaactgtca attgcaataa
     4561 tgtcgctaat ccacacataa agatatcaat tgaaatcaaa taagtcatct gttcttgatt
     4621 aaagcctagt cctgtaccga tcaaaagagg caccgctact gctcctgcat acatcgctag
     4681 cagatgctgc agccctaaaa ccgcagcttt tccattttgt gtttcttttt ccacgattat
     4741 gcgtcctcct caagaaactc aacttgtcca ttcgtcaagg aagcaatacg cgcaagagaa
     4801 acgactctca atcccatttc ttcaagtaac tgacgtccat cttggaaaga cttttcgatg
     4861 acgatcccga ttccttctac ttgcgctcca gcttgctggc ataattcgat caatcctttt
     4921 gccgcttgtc cgtttgccaa gaaatcgtcg ataatcaata ctttgtcttc tgaagataaa
     4981 aatttacgag aaatagaaat cgtacttgtc acttgtttcg taaaagaata aacagaagct
     5041 gtcaataatt cttcatccat cgtcaaactt tttgcttttc tagcgaagat cattggaaca
     5101 tctaattctc ttgcagcaaa taaagctggt gcgatcccag aagcttcaat ggtcacaact
     5161 ttcgtgattc cttgttcttt aaatacttct gcaaaccgtt ttcccatttg ttccatcaat
     5221 acagggtcta cttggtgagt gacgaagctg tcgactttta atacgccctc gcctaatata
     5281 cgtccgtctt tttgaatacg ttcaactaat tctttcactt tatttgctcc tttatcattt
     5341 attaataccg aaataaccgc ttttgtgcaa aaacctacta tgtttctgtt aaagaaaaac
     5401 accctccact ttatcataag aaaagaaggg tgaaatagcc aatcttttac ttatagtcca
     5461 gcatttacgg tactggctag aaactgtcat ccatattatg acgatatata agtaggtatg
     5521 taaaactaat aatagtgtaa attcattttc ttgcaactgt tttgcacgtg aaggcagttt
     5581 gccatatttg aataaaaaat gttgtttcaa atattgaaaa cagtctaaca ctactttctg
     5641 aaactgacaa cgtggtttac ttattttttt gtaaaatcta atgattttta atgcattatt
     5701 cctttttgtt cgggtttaaa tagttatttt tattttctcc tatctaaaaa agcagcccac
     5761 ataaaaagaa ggcatgacaa aaagcatgtc atagcccctt tttccgaata aatagtgttc
     5821 aaaagacagc ctatcggcaa taaggcaaga ttttataaac tacgggaagt aatttccgta
     5881 aatctattct tattctttta agctgacaag ctcgtcaatc actactgctt ttcctgaagc
     5941 aatagaatga tttgctgcga ttcctgtcaa aatcgacaaa ataccatcta cgtgagttgc
     6001 tgcccgattc cacggatctt cttctttttc ttcaccaaat agatcatcta ataaacgcgg
     6061 atctcctccg ccatgtccgc ccgcttcttg ttcgacgatt acctcatatg gctcaccgaa
     6121 catcggatag acatggatgg actgaagaac ggctgctcct tcatcctttt tatctccacc
     6181 tgcattgatg taagactttt cgatcacttt catttcgatc cgtcctttgc tgccattgaa
     6241 agaaacattg aatccttccc acggcatata cgcgtttagc gaatacgtca aaatcgcacc
     6301 agtttcatat ttgaccgtta cgcctagtgt atcttcaata gagatatcat cggaaaatac
     6361 actgcggtca cgaatatatc cactgtcttt ttctgcttct aaatacattt ctcgcaattc
     6421 ctcattggct gacaggtcta tagcaaatgg atcagcttta gctagttcgc ttccatagca
     6481 tcgactgtaa aatatttcta ctccgcgttt ctccgcatta tgcatcccat aaaagtttaa
     6541 tccaccgaat gcaaatacct tttggggacg gctgttcaac caaaaattca ctagatcaaa
     6601 atgatgggta gatttatgaa caagaagccc gccgctattg tgcttatttc gatgccatct
     6661 tctgaaataa tctgccccgt gttgcgtatt taacagccat tcgaagtgga tagaaaacac
     6721 atcaccaatc acatcacttt ctatcagttc ctttatcttc gtgtgatacg gtgcataacg
     6781 ataattaaaa gtgactcgca ggcttcttcc cgtctgtttt tggaccgtta ttatttcttg
     6841 agctttttcc tcatcgatag tcagaggttt ttcgcaaata acatcatatc cttttttcat
     6901 tgcacgaatg atataatcat gatgcgttcg atctactgac gtaataatga cttcagccgg
     6961 ccgtacgtca tccagtagtt gctcaaattg tgatggcata catgtcttta cttttttctg
     7021 tccatacttg ttcacaagta tccgatttgc ataatccatc cttgtttgag atacatcaca
     7081 aaaaccgact agttcacaag tttgatgata cgtagaggca attgcctcat aaaaaaaacg
     7141 cgctctccca ccaagtccga cttgtacata ttttttcttc atttaaagtc attctccttt
     7201 tcttttaagc aaaatactgt gacaaatggc agccctggat acagtaatgc agtgagtaaa
     7261 aaatcatgct gtatacacca gaacagtcca tacaaaaggc ataaatacca cagaaacccc
     7321 ttgacgtttt ttgacaacag ccagatattc cttttgatca aataaacaag actatctgct
     7381 ttatcttctt tgcagatatc aattagcgta ctaaaaaaac tatgggatac aaaaagcaac
     7441 agacacaata gaaaaggaat cagataccaa gctccaatct ttcccactaa taaataccaa
     7501 tcaactgcaa aaataatgac aaacagctga aacaacgtaa tgatgaaaca catccgtttg
     7561 atttttcctt tcctccaaca tacctttttt tctgccaagt aatggacaga tagataataa
     7621 gaaggataca tcatcaaaaa ataaatcaga tagacaacac gagcagacag cactaatcct
     7681 aaagtgaaca aacatcctag aactaaagcg aaaagagcat tactgataag actggttaaa
     7741 aaaatccgac agacatagta gttgagcttg taaagaaaca tctcttctat tggctttttc
     7801 atattcagcc cttcagaccg cttgtactga tcccctggac aagatattta ttgaagaata
     7861 agaaaatcag aaaaaccggt agcagcgaca acgtagacat cgcaaacata ctcgcgtaag
     7921 tcgtactaga tccagcgtct gcaaataatt tgatggcaat cgataccgtg taagatctcg
     7981 gcttgtttaa ataaatcaat ggtcccatga agtcgtccca tttccaataa aactggatga
     8041 tgatcgtcgt gatgatcgaa ggtttcagca aaggaaaaat aattttggta aagacactat
     8101 acttgctgca tccgtcaata atcgctgatt catctaactc tttggggatg ctgaccatga
     8161 attgcatgat ctggtaaatg aaaaatgctt gtccaaaaaa atgtggtaaa atcagcggca
     8221 catacgtccc aaccaaattc agccgattgt aaataataaa ctgcggaatc aaaatcactt
     8281 gtgcaggaat catcatcgtt actaacatga cgacgaacca gaatcttttt cctttgaaat
     8341 cgattctcga taatgcgtaa gcaatacaag tcgagctcag aactgtccct atcgtcgaaa
     8401 ctgcagagat gacaaacgaa ttcctaaaga aagtaaaaaa tgaaattcca gcaaaccccg
     8461 cccatccttt tgcgtaattt tcccattgaa aagtctctgg aatcaaattc agcgaaccac
     8521 tcataatctc ttgatccgtt ttaaaagaac ctgcgatcat ccataagacc ggataaatca
     8581 tgactagacc taatagcaaa ataaaaccgt attgaaacca tttcgttact gtattattca
     8641 tttcttcctc ctctcattca cacaacgacc catttttttg atgatttgaa aatcagaaag
     8701 gtgacagctc ccataatcaa cagcatgatc cacgaaatag cactagcata ccccatagaa
     8761 ttaaaagaaa aagcttggtt atacacataa agagcataaa agttcgtggc atcattcgga
     8821 ccaccttggg taatgataaa tgcctgcgta aaagtcataa acgccgcgat tgtctgcata
     8881 accaagttat acaaaatgat cggcgaaaga agaggtaacg tgatcttgta aaacgtctgc
     8941 catttgttcg ctccatcgat ctctgccgct tcataatacg agcctgggat ctctttgata
     9001 cctgcagcaa agatgatcat tgccgaaccg aattgccaag cagacatcaa aacaagaggg
     9061 atcatgacca gtttttgatc gctaaaccag gaaagcgcag gtgcgcctaa tcgttccagg
     9121 aaaatattca ctaacccgtt tttagaaaac aactcccgcc aaactagagc gatagaaata
     9181 ctgccaccga tcaaactagg cacataatac aaagaacgaa agaaagtcgt tcctttgata
     9241 ttcctcgtta aaagataagc aatcaataga ccaaagatca gtttcacagg tacagatgca
     9301 aatacataga gaaaagtgac tcggatggag ttcaaaaaac gggcatcact cagcatcttt
     9361 atataattat ccaagccgat ccatgagact ggatttccca aacgataatc ggtgaacgaa
     9421 taataaagtg acaagatcat cggtatcact gttatcaata gaaatcctag gatgaatgga
     9481 gaagcaaata cgtaaccgac cacattgtct tgatccaaga accgttttag tttttctttt
     9541 ttcatgcctt cctcctgctg ctatcctaga atgtttgacg caaactcata aagactctct
     9601 gctgcttctt ctgctgttac ttcttcataa atgacttttt caactaaaag attgtattgg
     9661 tcttgaattt ccggattcaa agcactatca aagacattca cttcgccgtt gtccatatcg
     9721 gaaatcattt ctaaataatc aaatgtcgct tgcaaagttg catcatctgc acgagcgagc
     9781 tgttctcgaa tattcgacat gattggtaca ccacgttctc cttttaaaat ttcattggct
     9841 tcttcactgt tttgaaagaa atcaataaac ttagcagctt cttctttatg ggctgagctt
     9901 tttgccatac tcagcatttg cgaagaacgt aaagggatac cagattctcc atctgctttt
     9961 ctgcgaggtg gattaatcaa ctgaatctcc atatcttcag gagcccctga caatatttct
    10021 gtcaactggt tacttgatag aaacaccatt gccgcttctc tatccctaac cgcctgaaaa
    10081 gacagatccg tcgtacttgc agctccacca cctggttctg gatgggcccc tttgtctacc
    10141 agatttttac gcagctggaa aaaatctgtt agatagtctg gattttcaaa tcccaagctg
    10201 ttagatagat cttcccgatt ataaaaattc aatcctttct caacttgtgg tatttgaata
    10261 atacagccag ctataaaatc ttcgaatctt gaaatagcat agattcctaa ttcttcagtg
    10321 atttgatagc tgatttgttc aaattcatcc catgtccaat ttgaagctgg ttcagggata
    10381 tttaattgtt caaatatatt tttattgtaa gcaatcgcaa acgaattcac cccgttagac
    10441 agtccaacta attgcccgtc gtaagtcgtc gttgctaaat aggattcatc tgcattagaa
    10501 atatcaatta tcccttgctc cacatattca tctaatggtt caatctgatt gatgtattga
    10561 ggaaattgat tgttcagttg gaaaatgtcc cagatatcat ctgatgctgc tagtgtacct
    10621 aattttgtgt aatatccttc ataatcataa aattcataat cgatcgtcac atggggattt
    10681 ttttcttcat aaagctcgat tacttttact gttgcatcgt gtcggacttg cgaaccccac
    10741 caagccatgt tcagcgtgat ttttttgtca tcttcttgcc cacaagccgt caagaagaat
    10801 aacataacac ttaaagctaa gatgattctc attcttttct tcattctctg cacctctact
    10861 tccattgtta tttttcaata tactgtattt ttatacagaa catatttatt aaacgatctt
    10921 tttcaagggg aataaaaacc aagacttgcc ttatcgttct gcctaggatg aacaactcaa
    10981 tactttcaag aaaaattaga tagtgctgat tcttctttca tcgctaaaaa cacttcttgc
    11041 ttcaatgatg ggaggataat tacgcaaaga aaaaaggata tcagacaact gcccaatatc
    11101 cttgacatag ctaaataagc atgcttcttc gtgtgcgctc ttccctaaaa aacagaaaaa
    11161 taactagtaa ttctgtatga aattaggtac aatatacacg tcgatgcaca agcattgact
    11221 atggcaactt gattggtcag ttcccgctga cttttcaagc cccgacaatt tagctgctac
    11281 taaattgtcg ggtgcctttt ttagttttta ttcgtttttc attctataga aagcgcttca
    11341 ataaatcaag acattttttt gttagaatgc taataataac aaaaggtttt aactcttctt
    11401 atgtacatta caggtaaaaa aacaaaaaaa tttctctttt ttttgaattc tgaacaaata
    11461 g
//
LOCUS       DACCQI010000093        11259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCQI010000093 DACCQI010000000
VERSION     DACCQI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.097"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..249
                     /locus_tag="IVO76_002124"
     CDS             <1..249
                     /locus_tag="IVO76_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7430066.1"
                     /translation="ALGRMSEMGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFY
                     TPLVPKRYDFLGEIGKVIQKKQSEPVPHEVKGTGSDLL"
     gene            431..1615
                     /locus_tag="IVO76_002125"
     CDS             431..1615
                     /locus_tag="IVO76_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7430067.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            1612..1902
                     /locus_tag="IVO76_002126"
     CDS             1612..1902
                     /locus_tag="IVO76_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430068.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            1899..2120
                     /locus_tag="IVO76_002127"
     CDS             1899..2120
                     /locus_tag="IVO76_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430069.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            2162..2941
                     /locus_tag="IVO76_002128"
     CDS             2162..2941
                     /locus_tag="IVO76_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP7430070.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            3008..3502
                     /locus_tag="IVO76_002129"
     CDS             3008..3502
                     /locus_tag="IVO76_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7430071.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            3558..4196
                     /locus_tag="IVO76_002130"
     CDS             3558..4196
                     /locus_tag="IVO76_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430072.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            4268..4663
                     /locus_tag="IVO76_002131"
     CDS             4268..4663
                     /locus_tag="IVO76_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7430073.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            4647..7100
                     /locus_tag="IVO76_002132"
     CDS             4647..7100
                     /locus_tag="IVO76_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7430074.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            7097..9124
                     /locus_tag="IVO76_002133"
     CDS             7097..9124
                     /locus_tag="IVO76_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430075.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            9121..10143
                     /locus_tag="IVO76_002134"
     CDS             9121..10143
                     /locus_tag="IVO76_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7430076.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            10160..11089
                     /locus_tag="IVO76_002135"
     CDS             10160..11089
                     /locus_tag="IVO76_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7430077.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         3821 a         1876 c         2255 g         3307 t
ORIGIN      
        1 gctttgggtc gtatgtctga aatgggcttt ggtatgatgt tcggaagcga tacacaaaaa
       61 caatttttct taaaacccat caagggaaga ggttacgtgg atacaggaaa aagtgtgatt
      121 agtgagtttt atacacccct tgtaccaaaa aggtacgatt tcttaggaga aataggaaaa
      181 gtcattcaaa aaaagcaatc tgagcctgta ccgcacgaag tgaaaggtac aggttcagac
      241 ttgctttgat tggtgtggcg ttagccacgc caatccttta gccccctcta tctaacaggg
      301 gggcacaaat acaactgaaa acaggcaatt ctcatcaacc aatgttgatg gtacaaagga
      361 ttgcggaaaa tatgaaaggt gtaaactcaa gggtcatagt ttacatcttt tttagattgg
      421 aggtttgcga atggaaaacg aaaaaatttg gattaaggaa ttgaaagaaa agcgactggc
      481 ttatggcgtt tctcaaaata aactggcggt tgcttctcat attaccagac cttacctatc
      541 ggatattgaa acaggcaagg cagtccctac tcaacaggtg aaagaagatt tactgaacgc
      601 tttggaacgc ttcaatcccg ataacccatt ggagatgtta ttcgactatg tacggattcg
      661 attccctacc aatgatgtgg ctcaaattat cggtgaggtt ttacgtttga atatggatta
      721 tatgctacac gaagactttg gctattattc ttatcccgaa cactatcgtt tcggtgatat
      781 tgttgtccta gtctctcatg atgtaagtaa aggtgtttta ttagaactga aaggaaaagg
      841 ctgtcggcaa tttgaaaact ttctattggc tcaacatcgg agctggtatg atttctttca
      901 agattgcatg gaacataaag gtattttcaa acgcttggat ttagctatca acgataaaac
      961 agggattttg aatattccag atttggcaaa gaaatgtaaa caggaagaat gcatttccgt
     1021 ctttcgaagc tttaaaaatt atcgcagtgg tgaattggtt caccgtgatg aaaaaccata
     1081 catgggaaac acactttata ttggctcatt aaaaagtgaa gtctatttct gtatatatga
     1141 gaaagattat gagcaatacg tgaaaaatga tgttccctta gaagacgcag aagtaaagaa
     1201 tcggtttgaa attcggttaa aaaatgaccg agccacccaa gccatgaaag atttattggc
     1261 acatcaacag gcggaaaaaa cagcctttga aattatcaat cgctatatcc gctttgcaga
     1321 taaagacgac actaaacgtc ggagtgattg gaaaactaat gaacgttggg aatggtttat
     1381 cggtaaaaat cgtggagcat tacgcctaac cacacagcca gagccttatt cttttgaacg
     1441 aacattgaac tggttacatc atcaagtcgc ccctacatta aaaatcgcaa gcattttaga
     1501 tgttctcaac ggtacaacca ttatctccac catgattcag gaagccaaac tgacagaaaa
     1561 acatgagaaa ttaattgaac aacaacatct agccatggag gatttgatta catgagcctt
     1621 ttaacaaaag aattaaagaa gttagggttt cagtgtggca ttgagtttca agcgtatata
     1681 caaaatacag ggaaatatac gtctctcatt attgagggaa aacggcaagc tggtgatacc
     1741 atctatacct atgattttta caaggtgaga ttttataata attataccaa tcgagtaacc
     1801 gtctatggag agcatttaac cccttttcaa ttattaaggc gtgtaaaaag ctatatctat
     1861 tatcgagaaa aatatcttaa ggaaaggagg acaatcacat gaattttggg cagaatttgt
     1921 acaactggtt cttggataat gcacagtcat tggtattgtt agcgattgtt gtcattggac
     1981 tgtttttagg ttttaaacga gaattttcaa aactcattgg cttcttgatt attgctctaa
     2041 tcgccgtcgg acttgtgttc aatgcagctg gcgtgaaaga tgtgttatta aacttattta
     2101 accgaatcat tggtgcttaa cagatatttt tcatgataaa ataggaagta tatattttgt
     2161 tatgaaagga tttatgaata tgtttacaaa tgatcaattt aaatctcttt taattaaaac
     2221 attagaaaga aagagctctt caaattatta tgaaggaggt aacgagattg tttctaaaat
     2281 gaaaatatca gaggtaactt tagatgagac tgacgatttt acttattata aaggatataa
     2341 aaagggctgg aatacgcttt gcacatatct taaaataaga gttccttttg atgatttaga
     2401 cttttttgaa agtcataaag atttgattac ccagacagca agctccatat atgataaaca
     2461 aggtgataat gtattagtgg atactatttt agtaccactt ccagagaatt acgaagtaat
     2521 taactttagt cagttgaaaa ttagtgatgt ggtttcacaa gctattgaag acgctgagtc
     2581 ttttatgagt aatggtgaat atcaacgtgc ctttgacaga gttcacactg cttttcatgg
     2641 atatttaatt gaaatactaa aaaaatatga aattacagtt cctagagacg aaaatctatc
     2701 taaattatat agtaggatac aacaattaat tgaaaaagaa attcaaccta ctgaacttgc
     2761 tgatatagtg aagacaacga taaggtcatc aaatggaatg attagctctc ttaatgaagc
     2821 aagaaatagg cattcacttg cacaccctaa tacaaatatt attggaaaga gagaagcaaa
     2881 actaataata ggtatttcaa gtacagttac agattatatt agcggatacc ttgataagta
     2941 gcgtcattaa ctgattgcta ctttttttat gactattttt ataaaaagga tgtgataaga
     3001 attggaaatg aaagtctatg tggcaaattt aggacggtat aatgaaggtg aattagtcgg
     3061 agcatggttt actccaccaa ttgatgagga agaaatggca gaaagaatcg gtttaaatga
     3121 ggactacgaa gaatatgcaa tacatgattt tgagcttcct tttgatgtgg acgaatacac
     3181 gccgattagt gaaatcaacc gtctatgtga agccattcaa gaaatcgaag gtacacctat
     3241 ttataatgaa ctcaaagaaa ttcaaggcat gtggtttagt agcttggaag aattactgga
     3301 aaacaaagag gatattcatt gttatagtga ttgtgattcg atggaagatg tcgcccgtta
     3361 ttacgtggaa gaaacaggac agcttggtga agtcccttcc aatttacaaa attatattga
     3421 ctaccaatct ttgggacgtg atatggaaat agagggtaat tacctagtta cctctcatgg
     3481 ggtatttgaa tattgtcagt aagtttaaca atccgtgtta taattaaatt aataaatagc
     3541 acggagaggt ataaattatg gtaaatgtat ttgaaaatct taaactatta aaactagaca
     3601 tgaaagataa aggctgggtg atagattcct tttactttcg ttacaaacag caaaattata
     3661 ttgtacttgt taagctattt gaaaaagagg aaaaagttcc agaatatgct ctgttaaaac
     3721 tagaattttt aaaggaaaac gatttttcag atatgctagc tgtctatgct aattcggtaa
     3781 aattatatac agacacgaaa acaataagag aatattttgg tatcgaatac agtagtaact
     3841 tgggagatgt tttatttcaa tttagccaaa ctcttgctcg atttattccg acagaagtta
     3901 gtgagaagaa aaatgaagac caaaaagaag ccatgtgttt ctcattaagt caaagtgatt
     3961 cagaagatcc cagaaaaaaa tattgttttt cagttagaag aaatccttta aagggaaatg
     4021 gtgagctggg aaaacgaagt ccttttaatg acaataaaac gagattgtat agacccagct
     4081 tatatgaacg attagggtca gataccaatt taagtttcag atattcaatg aatccagatg
     4141 atgaagaaac tgatgaaggt attattgcaa aatggacaaa aaataaaatt gaatagttaa
     4201 agcattgaaa gagcaatcac ctattttgat tgttcttttt ctatttttac ggaaaggacg
     4261 tgaagacatg aaaaaaataa gaagttacac cagtatttgg tcagtagaaa aagtgattta
     4321 tgcgattaat gatttacaac tccctttccc aattaccttt acgcaaatga catggtttat
     4381 cgtttctttg tttgtcgtca tactctttgg cagtttacca ccactttctc tgattgacgg
     4441 agcactactc aaatacttag ggattcccat tgttctcact gggttcatgt gccaaaagac
     4501 ctttgacgga aagaaaccct atggatttct aaagtctatc attacctatg ctctacgccc
     4561 taaagtaacc tacgctggca aacaaatacg attaaaaaaa gaaacaatga atgaatcgat
     4621 tacgattgta aggagtgaac aactagatgg catatccgat taaatatatg gaagataatc
     4681 tcgtatttaa tcatgatggc gaagtattcg cttattatga attaattccc tataattaca
     4741 gttttttaag ttccgatgaa aaaattcaag ttcatgataa tttctgtcaa ttgattgccc
     4801 aaaatcgtga tggcaagatt catgctctgc aactagcaac cgaatcaagt attcgagcca
     4861 cacaggaaca atcgaaaaaa gaaatcactg gacgattaaa agagatagct tacgaaaaaa
     4921 tagatgaaca gaccgaagcg ttaatttcta tgattggtga caatcaagta gactatcgct
     4981 tttttattgg ttttaaactc tacctcaatg aacaggaagt atccgtaaaa aatgttggga
     5041 aagaaattca aaaagcattt gcggaattct ttcatggtgt gaatcacaaa ttaatgggcg
     5101 attttgtttc tatgcctatg gcggaaatcg aacgcttttc taagatggaa cagttgttag
     5161 aaagtaaatt atccagacgc tttcagattc gtaaattgga gaaagatgat tttggttata
     5221 ttattgaaca tctttacggg caaacaggtg tcccttttga tgactacgac taccatttac
     5281 cgaaacgata cgaagataaa aaagtgttgg taaagaaata cgatattctc aaacccactc
     5341 gctgtttaat cgaagaaaat caacgctatt tacggattga acaggaagat accactactt
     5401 atgtggcgta ttttacaatt aatagtattg ttggtgaatt ggatttccct aatagtgaaa
     5461 ttttttatta ccagcaacag cagtttactt tccccgttga taccagtatg aatgttgaaa
     5521 ttgtcgccaa taaaaaagcc ttatctaccg ttcgcaataa aaagaaagaa ctgaaagatt
     5581 tagatagcca cgcatgggaa agcgacaacg aaaccagtac taatgtggtg gacgctttag
     5641 aatctgtcga tgaattagaa agtaatttag accaaaccaa agaatctatg tacaaactaa
     5701 gctatgtggt acgggtatca gcaaagaatt tggacgagct gaaacaacgt tgtgatgaag
     5761 taaaagattt ttatgatgat ctgtctatca aattagtacg accttttggc gatatgctag
     5821 gcttgcataa tgaatttatt ccctcaagca agcgatatat caatgactat attcaatatg
     5881 tcacgtcgga ttttttggct ggtcttggtt tcggtgctac acaaatgatt ggtgaaaatt
     5941 ctggtattta cgtcggttac aatctcgata caggcagaaa tatttatctc caacctagcc
     6001 ttgcaagtca aggaattaaa ggctctgtaa caaatgcatt agcgtccgct tttattggtt
     6061 cattaggtgg cggaaaatca ttctctaaca atatgctggt ctattattca gtactctttg
     6121 gcggacaggc aaccattgtt gacccaaaag ctgaacgtgg tgaatggaaa gaaaccttac
     6181 ctgatattgc ccatgaaatc aatattgtta atctcacgag tgagaacgac aataaagggt
     6241 tacttgaccc ttatgtgatt ttaaaactcc cgaaagattc agaaagcctt gccattgata
     6301 ttctcacgtt cttaacaggt atttccagcc gagatagcga gaagtttcct gtcctgcgta
     6361 aagctattcg gagtgtaact caaagcaaac aacgagggtt gttattggtg attgatgaat
     6421 tgcgaaaaga agatacgccc atcagcaata gtattgctga ccatatcgaa agctttgtcg
     6481 attatgattt tgcccatctg ttattcagtg atggcagtgt cacccaatcc attagcttgg
     6541 aaaaacaact caatattatt caagtggcag atttggtctt acccgattcc aatagctctt
     6601 ttgaagaata cactaccatg gaattattaa gtgtggcaat gctgattgtc attagtacct
     6661 ttgctttaga ctttatccac tctgaccgtt ccatttttaa aattgttgac cttgatgagg
     6721 cttggagttt tttacaagtg gcacaaggaa aagctctatc tatgcgattg gttcgagcag
     6781 gacgtgccat gaatgcaggg gtctatttcg ttactcaaaa cgcggacgat ttactagatg
     6841 aaaagatgaa gaataatatt ggcttgaaat ttgcctttcg ttccactgac ctagtggaaa
     6901 tcaaaaagac cttagaattt tttggtgtgg acaaggaaga tgaaaacaat cagaaacgct
     6961 tacgtgaatt agaaaatgga caatgtttag tttcagacat ctatggtcgt gtaggggtta
     7021 tgcagtttca cccgattttt gaagagttgc ttcatgcctt tgataccaga ccgcctgtgc
     7081 gaaatgaggt ggaagaatga cacaaaaaca aaagaaattt cttcactata gttggataac
     7141 gctcctttgt gtgggcgtta ttttactttt attaggcacg ctgggcaatg cagtacaagc
     7201 gacaggctta gttgatgaaa ccattgatac aagcaatgaa tactccaagt atggtttgaa
     7261 tcaatatcag cttgattatt atgtggacaa tagctggggc tggcttccat ggaattggag
     7321 tgatggcata ggacaatcgg tgatgtatgg attgtatgcc attactaatt ttatttggac
     7381 aatttctctt tatctatcga acgccacagg ttatttggtg aaagaagcct atgccttaga
     7441 tttcattagt caaacatccc aagcaattgg cgaaaacatg cagacgattg caggcattac
     7501 accgaatggc ttttccagtt cagggtttta tgtcggtttc ctccttcttt ttattctcat
     7561 tgttggaatc tatgttgcct atacaggatt ggttaagcga gaaacgacga aagtgatacg
     7621 agctgtttta aactttgtcg tggtgtttat tctatcggct tcctttattg cgtatgcacc
     7681 cgattatatt gcgaaaatca atgatttttc caaagatgtc agtaccgcca gtctgaatat
     7741 tgggacaaaa attgttcttc ctaacacgga tagtcaaggg aaagacagtg tggacatgat
     7801 acgtgacagc ttattctctg ttcaagtaaa gcaaccatgg ctattgcttc aatatggctc
     7861 cactgatgta gaagcgttag gggaagaacg tgtggaaagt ttattgtcta ccagtccaga
     7921 tacaaacaat ggtgaagata gagaaaatgt agtgattgaa gaaattgaag atcatgacaa
     7981 tacctattta accctaccga agacgatttc cagattagga acagtcttct ttttgtttgt
     8041 attcaatatt ggtatctcga tttttgtttt ccttctaaca ggaatcatga ttttttcgca
     8101 agtgctattt attattttcg ctatgttttt gccaatcagt ttcctattaa gtatgttgcc
     8161 gagttttgat ggcatgggca ggcatgccat taccaagtta tttaatgtga ttatgacaag
     8221 ggctggtatt accttgatta ttaccgttgc ctttagtatt tccactatgc tttattcgct
     8281 gtcagcaagt tctccctttt tcatgattat gtttttacaa attgtgacct ttgcagggat
     8341 ttattttaaa ctgggcgatt taatgagcct attcacttta caaagcaacg actctcaaaa
     8401 catgggcaga caagtgtttc gcagacctcg tcaaatgatg aatcgacaaa ccagaagatt
     8461 tcaacgcagt atgagtaaga tttttcgtgg aaaagcaggg tcaacgaagc aaaagacaat
     8521 gacaacaaag cagaatcaaa ccaataaagc taaccatact cgtcaaaatg aacggactac
     8581 accaaagaaa gaaacagcac tttccaatgt gaaaaagcaa caaggcggta tcgggcaacg
     8641 attaggtgaa aagacagccc atgtattgga cacgaaaaat cgtctagtca ataaaagaaa
     8701 gcaggcaaaa caacagatac aggcgacacc aacccatgtc aaatatgccc ttcatcaagg
     8761 aaaagagaat gtcactcata atgtaacaga ctttaaacaa tctatggcag ataccaaagc
     8821 tcaacgcaaa caacagcgta tgaaccagca ggagcaaaaa cgaaagaaca ttgctcaaaa
     8881 gcgtttggag ctggataaag caaaagaaaa aagacgaaaa gaaaacccat catctcctgt
     8941 tcagaataac cagcagcatg ttcgcaagcg tcagcaaacc gtcacgcctg ttttgtcaca
     9001 aaacactaaa cgtgaaaatc atcagcgacc attgaaaaaa gagcaaaaac aaaagacacc
     9061 accgaagcaa ttcaatcaga ggaagctaaa taaaagaacg gataagcgag gacaacaaac
     9121 atgaagctaa aaataacagt gatttgtagt gtggtcttct ctctcttctt ttttctctta
     9181 ttattcctag tcattttctt tgcagatgat acggacagtg gcgaaaacaa caaggattct
     9241 tctattccac aaggcggtgt gaccgtttca ccagaagtgt tggcacaccg tccgttgatt
     9301 gaaaaatatg gtaaggaata tgggattgaa gactatgttt cttatatctt agccattatg
     9361 caagtggaat cgggcggtac agcagaagat gttatgcaaa gttccgaatc tttgggttta
     9421 ccgcccaaca gtttgagtac cgaagagtcc atcaaacaag gggttaaata tttcagtgaa
     9481 ttattaacca gtgcagaaca acaaggagtg gatatagatt ctgttatcca atcttataat
     9541 tatggcggtg gctttttaaa ttatgtgagg agtcatggga aaaaatatac ctatgaatta
     9601 gccgaacagt tttctaaaga gaagtcgggt ggtcaaaaag cagactatcc caatcctata
     9661 gccatacctg tgaatggtgg ttggcgatat aactatggaa atcagtttta tgttcagttg
     9721 gtttcacaat acctaaccga cacttcccca acagaatttg atgatgaaac cgtgcaagtc
     9781 attatggacg aagccttgaa atacgaaggg tttccctatg tctttggagg ggcgtcgcct
     9841 accacctctt ttgattgtag tggcttaata caatgggtgt atgacaaagc tggcatttct
     9901 ctccctcgtg tcgcccaaga tcagtatgac gctacgcaag aaatctctat ggaagaagcc
     9961 caagcaggcg accttatctt ctttcattca acctataatg caggcacata tgtgactcat
    10021 gtggcgatat atttagaggg aaatcgcttt tatcatgcag gcgacccaat cggttatggc
    10081 gacttaagca gtcgttattg gcaagaccat ctgattgggg ctagacgtgt tattcataac
    10141 taagaaagga atgaatcaaa tgattataaa aatcgagcga aaagaaaaga aggagaaacc
    10201 aaagaaatct aaaaaaccga gcaagcaaag aaaagttccg atgattaaag ttggcacgca
    10261 taagaaatta acctttgttt tatgggtttt attgattggt agtgtcggat ttggaatcta
    10321 taaaaatttt actgccattg acactcatac tgtccgtgaa acagaaatca ttaaacagca
    10381 gattgtcgat accaaccaag tagaaagttt tgtgaaatcc tttgcgaaag actacttttc
    10441 gtggcaacaa tcccaagaag ccattgataa acgaaatgaa aaattgaccc attatttgac
    10501 ggaagaattg caggtattaa atgaagaaat gattcgtaag gatattccta ctagttctag
    10561 tgtgaacgat atacaggtgt ggcaggtttc tcaagtgaat gagaacacct ttgaggtact
    10621 gttttctgtt gaacaagtta ttaccgaaga caaagacaag gaaactatat cgtctagctt
    10681 tcatgtggtt gtccatatag atgaatcaga taatatggtt attatcaaaa atccgacaat
    10741 gagtaagaaa cctcaaaaat cggactacca acccaaacag ctagaaagtg accatacagt
    10801 agatacagaa acgatggacg aaatcactag ctttttagaa acgttcttcc agctctatcc
    10861 taccgccaca gaaaaagaat tgacttacta tgtaagtaat catgtgttac ctatgattaa
    10921 caaggaatat gttttcgagg aattggtgaa tcctatcttt accagaaaag ataaccaagt
    10981 aatcgtgaat gtcgctgtga aatatttaga tcaagaaaca aaggcaaccc aaatctcgca
    11041 atttgagttg atattagaaa aacaagataa ttggaagatt gtaaaataac aaatattggt
    11101 acatgattac agatactttg taatcatgta ctctttttga taaaaaattg gagattcctt
    11161 tacaaatatg ctcttacgtg ctattattta agtgactatt taaaaggagt taataaatat
    11221 gcggcaaggt attcttaaat aaactgtcaa tttgatagc
//
LOCUS       DACCQI010000094        10912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCQI010000094 DACCQI010000000
VERSION     DACCQI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.098"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(315..398)
                     /locus_tag="IVO76_002136"
     tRNA            complement(315..398)
                     /locus_tag="IVO76_002136"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..1426
                     /locus_tag="IVO76_002137"
     CDS             926..1426
                     /locus_tag="IVO76_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7430078.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1616..1960)
                     /locus_tag="IVO76_002138"
     CDS             complement(1616..1960)
                     /locus_tag="IVO76_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7430079.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2114..3034)
                     /locus_tag="IVO76_002139"
     CDS             complement(2114..3034)
                     /locus_tag="IVO76_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7430080.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3221..3712
                     /locus_tag="IVO76_002140"
     CDS             3221..3712
                     /locus_tag="IVO76_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7430081.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3736..6111)
                     /locus_tag="IVO76_002141"
     CDS             complement(3736..6111)
                     /locus_tag="IVO76_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7430082.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6437..7063
                     /locus_tag="IVO76_002142"
     CDS             6437..7063
                     /locus_tag="IVO76_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7430083.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7086..7790
                     /locus_tag="IVO76_002143"
     CDS             7086..7790
                     /locus_tag="IVO76_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7430084.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7982..8815
                     /locus_tag="IVO76_002144"
     CDS             7982..8815
                     /locus_tag="IVO76_002144"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7430085.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9012..>10912
                     /locus_tag="IVO76_002145"
     CDS             9012..>10912
                     /locus_tag="IVO76_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7430086.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPL"
BASE COUNT         3344 a         2061 c         1950 g         3557 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaaataaa aaaaatcttg gcagtcttaa agcacaacaa aaaaatgggg ctgcgattta
     1381 tcgtgaagat gagaaagaat actatacgaa aatcaaactg atgtaaaaaa gaaggattcc
     1441 aaaaggaaag ggatttgaat gtcctttatc aatagaacac atttatttaa ttaacttata
     1501 atatagcaga caaaatttta taaacaaaga aaccttttat ctcttttctt ctagaaagga
     1561 taaaaggctt ttttgttttt cctatttgct tctcatagct agtcagccaa gatttctatc
     1621 gtatccccaa cgcaatccgt gcataacggc tcatcttatc tgtcgtccat gctggatacc
     1681 agactagttt gacttctgct ttcgttactt ctggcacttc ttttaatgcg tcatggatag
     1741 attctgttaa aatatctgct aatggacagc ccatagttgt tagagtcatc ttaatgactg
     1801 tttctccatt ttcaggattg aattctatat cataaatcaa gcctaaattg acgatgtcga
     1861 ttcctaattc aggatcaatc accatttcca aggcagcaag aatacgttct ttgattagtt
     1921 ctatttcttg tccagaccgt tcttggtttg cttcgctcat cgtacgacac tccttaacac
     1981 gatttttatt tatcatactc tttttttagc tgcttgtaaa tgagaattta gaagtaagat
     2041 tctcgtttaa atttataaaa atgttcttat tagatgtaat agattaaatt tattctaaaa
     2101 taaacctgat tcctcatttt ttttgtgaac gattttttat ttctggcaat acgccagttg
     2161 ttcgatacat tttttgttcg ttttcatttg caaaccggcc aaatactgaa tgagttggta
     2221 aaatattgat aaatgcatcg tcgtcgattt catgaacgat ctgatcaaga tcatacattt
     2281 cgtagcgggt gatgaccatc atgatcatgt tcccttgttt ttttgaatag ccaccgacag
     2341 acggcagtaa tgtcattcca cgtaccatcc gttttgctag ttcttctgtt acctcttctg
     2401 gcttgacggt aacgatcatt gctgttactt tttgatgact cgtgtggatg gcatcaatta
     2461 cttgagtcat tgcataaatg gaaataatcg tatacaaggc actttcccag ttaaagaaga
     2521 agcctgcgat gactacaatg ataccattta gaatcagcat gtagtttccg actgtttttc
     2581 cagttgtttg tgaaagtaca agagagatga tatccatccc accagttgta aagcccattt
     2641 ttaaagataa tcctacgcca agacctaata aaacaccgcc tactagggca ttcatcaaaa
     2701 tattatcagt gatctgtccg actggaagca gcatcgtcat caccgataca catacaacat
     2761 tcacaaaact aaggatcgtt gctcgttttc ctactttgag aaaccctaat acaaacacag
     2821 ggatattcaa aagaaaaata aatgttcctg tgtctatctt gatatgaaac cattcatata
     2881 aaagcgagga aatgatttga gcgatcccat tcatccctgc agaaaagaca ttggctggaa
     2941 tcaaaaaata atttaaagcg accgcagcgg tcaatccggt tatcaaaata accactattt
     3001 tctttaatct ttcattttta tgatacgcag tcataatttt actcacaagc acctctcccc
     3061 tactttggta ttccaaatgt aagaataaca gtttatctat taaaataaaa tacttcgaac
     3121 tcaaatgaga agaattttag caaacaggta agaatctgcc ctaatcctat gataagatag
     3181 atgtatggta aatatttata tgtatatcag gagggttcct atggcaaaag atgctagctt
     3241 cgacattgta tcagaagtca atacagaaga agtaaaaaat gcgattcagc aaacaacaaa
     3301 agaattgtcc aatcgctttg acttcaagga ttctacagta gaaatcaaat tagagaacag
     3361 caatctagtt gtattgagcg atgatgattt caaaatcgaa cagatcaaag atgtattatt
     3421 tggtaagctg atcaaaagaa atgtgccaat caaaaatatc catttttccg aatcagaaca
     3481 cgctctaggt ggcaaagctc gacaaactgc cgatttggtc aacggaatcg atcgtgaaaa
     3541 cgcgaagaaa atcactacag ctatcaaaaa tgaaaaactg aaagtaaaag cacaaatcca
     3601 agaagatcag attagagtga caggtaagaa ccgtgatgat ctacaagctg tgattgcttt
     3661 attacgtaaa cttgacttac caatcgagtt gcaattcact aactatcgtt aatcaaaaaa
     3721 cgaggctgac gtctgtcatt cctcgttttt ttgatttact gcttcttttg ataaccaaaa
     3781 acgctgtatt ccttcttcca attccccaac acttcgtact tctcttgctg tttgccaatg
     3841 ttgtgggaat aaacgtcgat aagaaaaact agaaaatcgt tgatctgcca atacaacgat
     3901 tccttgatcc gccatgccac gaatcactcg accaccagct tgcaagactt tgttcattcc
     3961 aggaagctga taagcgtaag caaatccaag gtgttccttc tcatcataat atgatttgat
     4021 cagttcctgc tcatgattca tctgcggcaa acctacacct acgatcatac tgccgatcaa
     4081 tcggtcccct ctcaaatcga ttccttctga aaaaatcccg ccaagtacgc aaaatccaat
     4141 caatgtttca gcgggatttg tttgaaattt tgttaaaaat cgttcgcgtt cttcctcatt
     4201 catgtccgtt tcttggatta gcacattgct gtcgggatat tgctgtctga atgcttccac
     4261 cgcaagatct aaatattgat aagaaggaaa gaaaacgaaa taattcccga cctttccatc
     4321 agcaaaagca tgaatcgtct ctactaattt tgccagactg tgttcccttt ttcgataagt
     4381 cgtctctagg taatttgcga tgaaaacttg ctggtttcct tctgggaaag gactcggcag
     4441 tttgtaagcc agactttttt gtccccccag tgtttcctga taataagaga gtggtgaaaa
     4501 actagcagaa aaaagaatac tgctttttcc tttatccaac gactgctcaa gaaacaagct
     4561 tggatcaata caaaactctt ttactatcag atcgcgatac gttttttcga ctgttgtttc
     4621 gtagtgatca tcataaaatt cactgacctt caaaaaatga agcaaatcaa aataataagg
     4681 aagcaattgt tcttgtgatg ggtgctcagg aaattctgcc agccattctt taattttttc
     4741 gcttaattga taacctgcct ttactaaaga ttcagccggt gctttttgat gatgataatc
     4801 ccaatgatct tcttttgcta tttctcggat cgaatcaaat tcttttaata gtttgttgaa
     4861 acggcgatga agttttttga attcctttgg tatagcttcc ttacttcgtt tcgttttttc
     4921 ataagatagt tctgccgaat acatctctcg ggaacgattc accaaattat gtgcttcgtc
     4981 aatcaaaaac agatagtcct cgtttttttc atcttcaaaa aaacgtctta aataaacagt
     5041 tggatcaaat aaataattgt aatccccaat gattacgtca caccagatac tgacatctaa
     5101 tgatagttcg aaaggacaaa gtgtatgctt tcttgcataa gtttcgatga cttcacgagt
     5161 gatctgattc tcatgattca aaagatccca taaaccttca ttaatccgat tgtaataacc
     5221 attggcatat gggcactgat ctggattaca ggtcgtctca tctaaaaagc agatcttgtc
     5281 tttcgccgtc aaagtgacac tttttacttc agctcctgta tcttttaatg ctgtcatagc
     5341 atcttcagct acttgtctag ttattgtttt agccgttaga taaaagatct tttctccctc
     5401 ttcttcccct acagctttca aagcggggaa taacgtagag attgtttttc ctgttccggt
     5461 aggtgcttct acaaacaatt tctgtttcgt acggatcgtt ttataagcag ctaccgctag
     5521 ttcacgctga cctttcctat actcatcatg ggggaatttc aaagccatca aagaggcatt
     5581 gcgtacagtg cgccagtttt cttgaaaaac aagccattct ttataatctt cagtcagctt
     5641 tttaaaaaaa gtttctaact gctctcttgt ctgatgttca acctttctcg tgatcacttc
     5701 ttctgccgtc tggaagtaag tcaattgcag atttatttct tgtaaatcat gttcttgact
     5761 atagatatag gcatacaccc ttgcttgatg aaagaaaagt tccacttgtt ctggttctaa
     5821 atcttcaaat cgtggttcgg aggttttgat ttcatctata taataaatac cgtctctttt
     5881 gaaaataccg tctgcacggc cttcaacgat caggtaatat tcatctaatt gaactgttgt
     5941 ttttaaaaag acttcttttt catagttttc tcccgcatct ttttggagct tgcggtggat
     6001 tctcgctcct tctaatgcag tatggttact ttttttacga ttgtcgatac ttccacgtcg
     6061 caaaataaat tctactaagt gccgcacaga tatttttgac tgtttgatca tttccaccac
     6121 ctctttctct ctaagattac ctgaaaaaac ctagtttagc aaaaagagct ctctttccaa
     6181 acataaaaaa acaccctgct gattaggcag ggatatagga tatagaattt ggatttttgg
     6241 atgaatctaa tgtaacaagg acatattaaa atgctatgaa aacttacttt ccttctcttt
     6301 agaatatcca aatacttttc atatagtctg ataattcaga taagtaaaga gaaataagta
     6361 cctgtttttg tttcctagca atgaatgcgt tatactaatg gatgaaagaa gttttggcta
     6421 tcaaggagga atgcacatgc atatcgaaag attaaaaaca ggaatgattg aagaaaactg
     6481 ttatttggtc tacaacgaag aagcactttt gatcattgac cctggagaag atgcggaacg
     6541 aatcaaacaa gaaatcaaaa aaacaaacca acagccggta gcgattttgc ttacccatac
     6601 tcactacgat catatcggtg cagtggaacc tttacgcgcg tattatcaaa tcccagttta
     6661 tgtcagccca ctagagcaaa aatggctagg tgatccgatt ttgaatcttt ctggactcgg
     6721 tagacatgat gatattgctg atgttattgt ctcgcctgca gaatatgaat ttgaaatgaa
     6781 aacttatact ttaggaaaca tgtcttttcg cgtcgtccct accccagggc attctatagg
     6841 aagcgtcagc tttatttttg atgattttgt cgtttctggc gacgccctgt ttagaggaag
     6901 tatcggtaga accgatctgt atacagggaa tttagaacaa ttacttcata gtattcggac
     6961 ccagctgttt gtattacctg atgaatttgc agtctatcca ggacatggcg atgcaacgac
     7021 gattgaacag gaaaaaagga caaatccttt tttcaatcgt taaaacgact taggaggaga
     7081 ataaaatgac agaaactaca ctttatatcg tacgacacgg aaaaacgatg ttcaatacaa
     7141 ttgaacgagt acaaggctgg tgtgatacac ccctcacaaa gcaaggacaa gaaggcattc
     7201 attatttagg tcgcgggtta aaaaatgtcg acttttcatt tgcttattct agtgacagtg
     7261 gtcgtgcaat cgaaactgcc cgtattattc taggagaaca ttctaaggga aaagaaattc
     7321 cttattttat tgaaaaaagg attagagaat ggtgtttcgg gtctcttgaa ggtggatatg
     7381 acatggaaat gtggggtgtg atccctcgtg tattgaattt taaaacatat gatgaaatgt
     7441 ttaccacaga agtttctttt gaacaaattg ctaatgctgt atacaactta gataccgctg
     7501 ggtgggcgga aacttatgaa acattgcgag accgtgtatg gacaggattt gaagatatcg
     7561 cccatcactg cgaaaaaaat ggtggtggaa atgttttagt cgtatctcat ggtttgacga
     7621 tcgccttttt gcttaatctg atcgatccta aacagcctgt tcgtgctggg cttttaaatg
     7681 gcagcgtaac aaaggtcact tatgcaaacg gtcgattttc tatccacggc atcaatgata
     7741 cgtcttatat cgataacggg aaaacacata gtcgcgactc gtttctataa tggggatatt
     7801 ttaattttta ccagagaatc ataaaaggca aagtctgaac gagggctttg tcttcttttt
     7861 tctcttcgtg tacttatctg ctttatttct caagtttttc ttaatattaa aaaacagatt
     7921 gggtttctca aaaaataacg gtatactata agatgtatct taattgggaa ggaaggaatt
     7981 tatgtttttt gcaaatatta tcaaagcagt gattctcggt attattgaag gaataacgga
     8041 atggctgccc atcagtagta ctggtcattt gatcctagca gatgaattca tcaaactgga
     8101 tgccagttca caatttatgt cgatgttcaa tgtcgttatc caattagggg cgatcttggc
     8161 tgtcgtcgtt ctttattttc acaagttaaa cccattttct ccaagtaaat caacggtaga
     8221 aaaaaaagat acgtggacac tttggtttaa agtgatcgtt gcttgcatac cagccatcat
     8281 cattggtttg cctttggatg attggttaga agcaaatttc cacaagttct taccagttgc
     8341 cattgttttg atcgtttacg gtattgcctt tattattgtc gaaaaacgca acaaaaacaa
     8401 aacacctcgt tggacttctc tgaatgactt tacgtaccaa gcagctcttc ttgttgggtt
     8461 attccaagtc ttggcacttg ttcccggaac atcccgttct ggcgcaacga tcctaggatc
     8521 tatcttgatt ggtgcttcgc gttttgtcgc tacagagttt tcatttttct tagggattcc
     8581 tgtcatgttt ggcgcaagcg gcttgaagat cgtcaaatat ttagctgaag ggaattcatt
     8641 caaaatggaa gaaactgttg ttcttcttgt aggaacattc gtatcttttg tggtttccgt
     8701 cttagctatc aaattcttac taagctattt gaagaaaaac gactttaccg cttttggctg
     8761 gtatcgtatt atccttggta tcattttgat tggttattgg tttattgcaa tgtagaaaaa
     8821 accagaacac ttctcttatt tgagaaatgt tctggttttt tattttcttt aataataatc
     8881 attataaata ataaaaacaa catcaagctt aatcaccaaa tgataaccat tatcgttctc
     8941 atttagctgt tattattatc aattacattt ttattatgct atccttgttt tgacaaggag
     9001 gtctaataac gatgactaat cagaaaaaag cttttgcagc aacaatcggc tgccttttat
     9061 ttatgatctt cggttttatc ttcgaccgat tctctcttac catcacccca cttttttata
     9121 tcagtgccat tatcatcggt ggatataaac aaacatcgga aggcttgaaa gaattgtgga
     9181 atgatcggac actgaatgtg gatttattga tggctttagc tgctattggt gcctgtttga
     9241 tcggtaatta ctttgaagga gcaatgttga cattcatctt ttgtttaagc ggtgcattag
     9301 aggaatacac aactaataaa agtcaaaaag aaattactgc tttgatgaat cttcaaccac
     9361 aaaaagccca attattaaaa gaaaatggac aaatgcaaga agtagatgtt tctcaactaa
     9421 aggtggataa cttggtattt gtcgctaaag gagcagctgt tccgatagat ggctttttag
     9481 aaagcacaca ggcaacgatc gatgagtctg cattaagcgg tgaatctgtg cctgtagaaa
     9541 aatatttagg cgatgagatg tatgcaggaa cgctgaatca aggaaatccc atcacgattc
     9601 gagtaacaaa aacgagttcc gaaactgtct ttgctaagat tatccaatta gtagaagaag
     9661 cgcagaatac gccaactcaa acagcttcat ttatcgaacg aatcgaaaat aattatgtaa
     9721 aattgatttt gcttgctgtt cctctaatga tccttctccc ccactttttc ctaggttgga
     9781 gttgggatga aagcttttat cgaggaatgg tattattagt tgtggcttct ccttgtgcgc
     9841 ttgtcgcatc tgctacacca gcttctctag ctgctctttc aaattctgca aaaaatggga
     9901 ttttaatcaa aggcggtatc catttagaaa agctttctga attaaaagcg attgcctttg
     9961 ataaaacagg aacgctcact aaaggaaaac cagttgtaac tgacagcttt ttctttgatg
    10021 aaaaggattt ggcacaacaa cttcttgttg ctatggaaca aaaaacgacg catccacttg
    10081 ctcatgcgat tttgcaatat ttcgattgca caatacctga agaaatccaa tacttaccaa
    10141 tagaagaaat tactggtttt ggattacaaa ctacctattt aggtgcgcaa tggaaagttg
    10201 gcaagcacgc atatgatcct gagacgatga taatcagcaa agagattgcg gaaatgatcg
    10261 aacgtttgga aaatcaaggc aaaaccgtga tttatctttc taaagatcag cagttaattg
    10321 ctgttttagg tctgctagat attccaaaag caaataccca acaagtaatt tcctacttta
    10381 aatcccaaaa catccataca agtatgataa ccggcgacca ttctggcaca gcaaaggcca
    10441 ttgctgaaca agtagggatt gaacattact atgcaagctg tactccggaa gaaaaaacac
    10501 aactagtcaa aaaagagaat gaactttacc aagtaaatgc gatggttggt gatggagtca
    10561 atgatgcgcc tgctttagca gccgcttctt taggtgtcgc tatggggcaa gggacagatg
    10621 ccgctatgga tatcgcagac atcgtcttaa tgaaaagcga cttaaataaa tttgtctaca
    10681 gtcatcggct atctttaaaa atgcggcgga tcatcaaaca aaatattatt ttttcgattc
    10741 ttgttattct cttgttgatt gcttctaact tcctgcaatt tttaaatcta cctcttggag
    10801 ttgtaggaca cgaaggaagc acaatattgg ttattctgaa tggcttgcgt ttactaagac
    10861 ctttacctga tctttctgat tcttcaaaaa aatgtaagtc ttgtccacta ta
//
LOCUS       DACCQI010000095        10908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCQI010000095 DACCQI010000000
VERSION     DACCQI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.099"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(551..2164)
                     /locus_tag="IVO76_002146"
     CDS             complement(551..2164)
                     /locus_tag="IVO76_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7430087.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2305..3213)
                     /locus_tag="IVO76_002147"
     CDS             complement(2305..3213)
                     /locus_tag="IVO76_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430088.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3396..4520
                     /locus_tag="IVO76_002148"
     CDS             3396..4520
                     /locus_tag="IVO76_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7430089.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4747..5664
                     /locus_tag="IVO76_002149"
     CDS             4747..5664
                     /locus_tag="IVO76_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7430090.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5657..6490
                     /locus_tag="IVO76_002150"
     CDS             5657..6490
                     /locus_tag="IVO76_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7430091.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6573..7691
                     /locus_tag="IVO76_002151"
     CDS             6573..7691
                     /locus_tag="IVO76_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7430092.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7685..9583
                     /locus_tag="IVO76_002152"
     CDS             7685..9583
                     /locus_tag="IVO76_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7430093.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9577..10800
                     /locus_tag="IVO76_002153"
     CDS             9577..10800
                     /locus_tag="IVO76_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7430094.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
BASE COUNT         3670 a         1722 c         2122 g         3394 t
ORIGIN      
        1 taaataaata ggaacaaaaa aattaaatta tttaatacac acattaggga tcattcattg
       61 aaggattaga agacaacgag gatggcgaaa aatatgatta caatagaaaa gtcgttctaa
      121 accaatcagt tcctagtatc acacaacgca agccagtcga tttggtgaga ttagaggagt
      181 gaaagcaaac tttattatat aagaaaaaat attcgaaagg gatgctagtt taggtactag
      241 tgctgataga ttaatatgat gcctcgtaat attaggcgta gttactctag agacatcact
      301 atggactatg atttgtctca gagtcacaac atgttttttt atgaaaagaa acagctaaaa
      361 taattaaatt gcgcaatgtt taaactttct taaactataa cctaataaat gtgaagaaat
      421 aatgcacaat ttcttcacat ttatcttcta ttctatatac ataccaacca acaaccctat
      481 atactttttc atttttactc ctcaagtaaa aataactcct agaccgcggc tacccaccgc
      541 ggtcgttttt ttattcttca atcaaaatcg ctttgatacc caaacctgcc aagattcggg
      601 cagaagtata agtattcaat cctcttctga aagtaatgta aactttttta tcttttggga
      661 tttcattgat tcgatctcgt aactcattca ttggaatatt ttttgttgcc gagatactgc
      721 cagaaggtgc tttattaggt tcacgtatat ctaaaaagaa cgcttctttc aaatcgtttt
      781 cgggtatatc acttgctttg atcgtttcta cgatattcgt catttgattg attgcgacat
      841 aaccagctat attcaacggg tcacgggtag tagaatacgg tggagagtaa ggcaactcca
      901 aatcaggcaa atcaaagact gtcaagtttc cggtgattgc tacagaaagt tctcccatcc
      961 gtttatcgac acctttttct ccaaatgctt gtccacctaa aatacgaccg ctatctgcat
     1021 caaaaatcag ctttagattt aatcttgtag cttctggata gaaataggca tggtcaaagg
     1081 gagtgataaa aacagtttta taatttgtta tgcctgatgc ttttaattca tgttctgtca
     1141 ttcccgcata acttgctgta tgatcaaaga tttttgcgac acctgcacca atatatccgc
     1201 gatagcgcat aggcgtacca tttaaaatat ctgccagcat atgtccttga cgattggccg
     1261 cactggaaag catactttgg atcggctgac cagtcactac actagtcgtt tcgataatat
     1321 cgccaatcgc atagatgtcc ggcagattgg tttgtaactg gtcatcaaca atgatctgac
     1381 ctgtatctga taattgtatg ccggttgctt tcaccacttc attcttcgga gaaacaccaa
     1441 cagcaaaaat tagcatatca gcagaaagga cggagccatc tgataatact atttctcgac
     1501 ctttatcacg gatctcagta actcttgttt ctaaatgaac ggctaatcct tctttcagca
     1561 atttgtcata aactaaatta gcaatttctg gatcataagg gaaagcgact tgtggcaatt
     1621 gatcgattaa agtcacgtct aagccgcgat gcttgaaatt ctcagctaat tccagaccca
     1681 taacacctgc accaagaatg acgacatgtt gtggattttt ttcttcaagg aacgatttga
     1741 ttcgatccgc atccgtcaca ctacggagga caaagccgtt ttctgcttca aaaactccgg
     1801 gaatatccgg ataatctggt ctagcaccag aagagatgat caacttatcg taatctgttt
     1861 ttatttcttc atttgtggat aggtctttaa ctgttaatcg cttagtagaa ggatcgattg
     1921 ctgttacttc gtgtttagta aagacatcga tgttgttttt tgttttcaat atttctggtg
     1981 tacgttcgat cagcgagtca cggtcagtga tcacacctcc taaataataa ggcaaagcac
     2041 aacttgcata agaaatattt tctccgcgtt catagataat gatttcgtgc gcttcattca
     2101 atcgacggaa acgagtggca aatgaaggac cgccagctac accgccaatg acaacaactt
     2161 tcataaaaac atctcctttt ctacgttgat cgtactatgt tatggtaaaa aacagcaaat
     2221 aattgtgccg gaaaattatt gtttattttg aatagacaga aaatatagag aaataatttg
     2281 tggataaaac ctaaaaattt ttttctattt taaggataga taatactttg aaggggataa
     2341 ccctgtatat tttttaaaag ctgaagaaaa atgatggatc gaatgaaatc ctaatatatc
     2401 tgcaatttct gtataggtta attcattttc tagcacgaga cgttttgctt tttccatttt
     2461 tagttcttta aaatagctaa taacactttt tccattcact tttctaaaat gtttttttaa
     2521 gctggctgga ctgataaaaa aaatttgggc taattgttca agcaaaaaat tttctgaaag
     2581 atgttcttct aaatatcgta tgattggatc aactaattca tctgaataaa gaatatgctt
     2641 ttttttagga atgactttct catttgaagg tattgtcaat aaagcaagtt cctgttgata
     2701 atgccttggt aaggaagaca attgaatttt ttgcagacaa taagtaacta ataaggaaag
     2761 caaatgaatg ctcatctgtt ggcatagtat gaaaaaagaa ttgatttcct gatcatagga
     2821 aatagctggc ttactatgtt ttagataatc aattttgaac tgttcatttt tcagaaaagt
     2881 aatgatagat gttatttctt gatctataga aaaagattgt cccgtaatat gtgctaataa
     2941 ttgctcatca aaataaaact ggataatgat aaaagtttgt gtttcctttt gagtagaaaa
     3001 gatttggact tcttgctgtt tatcaatgaa taaaagggaa ggattatcta gttctataga
     3061 tttatgtgca aattcagcat ataaattagc gctttcattt attagtagtc gaaaataggg
     3121 agcagcagat aaatgaagat gggtatctat ggaataggtt agtgtttttt tgacaagatt
     3181 ggaggattgg ttgtttgtta acaaaagaac cactttcctt tctcttttta tataaaaagc
     3241 tttctattta taattaatga ctattataac aaaaatgaaa agccaaaacg acaagtaaaa
     3301 tattttttta tataaatatt tgtgttacta attccgttaa tatacataag tgaaagcgtt
     3361 ttatataaga acttgaaaag aaagaaggga tagacatgaa aaaaagatac aaattggtga
     3421 tagctttgct aaacattacc ctattattca gcttaagtgc atgtggaaaa actaacgaag
     3481 aagtaacagc aaacatcgca gatccaatga taaaaaaagc cactacatta gaagtatatg
     3541 tcgcaaacgg caaactgaca aactatgttg aaaatgctgc aaaaatctat aaccgcaaaa
     3601 ctggagcaaa tattgactta aaaattgtga atgtagcttc gggaacagca acagtacaaa
     3661 tgatcacccc aaaattggta acacacgaag atatgccgga tattatctcc atggcagaca
     3721 cttcagcagc aggtgtgctg gaaaaatttc aagatgcttt ttatagtgct gatgaatatg
     3781 gattttatga caaatatggc gatcagtttt atgatcaaaa attgaatatt ttgcgtcaac
     3841 aaacgagtaa tgaaacgatt attccttggg ctagtgattt taccccagca ctttgttatt
     3901 atcaaccaaa aaaatttgct gaaataggtg tagattttga agaaatcagt tcttgggaag
     3961 aatatatcca ggtggcaaaa gcattaaaag aaaaaactgg tagttttgga attgccttgc
     4021 cagaatctgg ggaccaagaa ttgtttatca acatgctagc ccagcagaat caatctttgc
     4081 ttgatgaaga aggaaatatc cgtctgaata ctaaagaagc gaaacatgct gcaaagttaa
     4141 tcaaagaaat gatcgactca gatattgtac atttctacgg tgcacaagat gctgagaaag
     4201 catttcaaga atctgcaatg tttgttgctg gcggctggta tgctacgaat atgtctttga
     4261 attttcctga tgcatctgac aaatggcgca tggctccact gattcctttt tcaaaagaaa
     4321 atccaggaaa aggtgcagta tcaggaggat caagttttta tgtccctaaa gatgccaaaa
     4381 atccacaagt ggcacaacaa tttcttacat ttatgctgac agatgaagaa tgcttagcca
     4441 acgcactgga gctgggcgtg gctacatcta actcattggc ctatcaaaca gaagcagctg
     4501 agaaaaagtt tgactactaa ggaggacaaa aatactatga actattgaat aaaatcaatg
     4561 aaaatacatc aaatactgtt ttaccagcca cttattcaga tgctacgtct tatttgacta
     4621 ccgctgcata taactattgg caaacaggtg attttgaaaa ttcatatcta aaagaagcag
     4681 aaaattttgc tcataagtac ggcgtaaaat taactggtga ttaaaaaata ttaggaggac
     4741 ttaacgatga tgcaatcttc gttaccaagc aagacaataa agaacagcaa gaaaaaagca
     4801 acagatacgt ctcataaaaa aagtgcttat ggattggtca caccttttgt tattttcttt
     4861 attgtgacga ttttgattcc catcattatc tcgtttgctt ttagttttat gaaaattggc
     4921 tactcatttg aatttgttgg atttgacaat tatctagcta tcttccgaga tccattgttt
     4981 attaaatctt acacgaatgt tttgattatc atggcaggat caattccaat aacgatgcta
     5041 ttagctattt actttgctgt tttattgaat tcagcacagt taaaaggaaa aggatttttt
     5101 agaactgtct attatattcc aaccattaca tcaacggtgg ctgtagcttc cgtattcatg
     5161 acttttttca atccgactgg gttattcaat ggatttttga atgcgctagg aattaacggt
     5221 gtacagtggt taacaaatcc tttttggata cgaatatcaa tgatcatcac gatgatttgg
     5281 cttagtgtag gatataacac tattttattt ttagctggtc ttcaagggat ttctaaagag
     5341 atatacgaaa gtgctgaaat agatggagca tcgaaatttc ggcaattctt tttattaacg
     5401 atccccatgc taaagccaat cattttaatg gccacagttt tagccactat caatggcttg
     5461 ggtaatttca atatcccaaa tatattcttt gggacttcca atggaccaga aaattcagct
     5521 ttggtcgtag gagtcaattt atataaaaca agttttgaaa tggttgattt tggaaaagcc
     5581 tctgctattg cctggacgat ggtgtttgta tctgttatat tatcggctat tcaatttaaa
     5641 ttcggaggtg ataaaaatga ctaaacaaaa gatacaaagc agaattttta cgtacgtcat
     5701 tattgtgttt ggtgccttaa tatttttgat gccatattta tatatgattg tagcttcaac
     5761 gcaaagtaat caagaaattt taagtggcgg aatcaatttc aagtttgggg atatgctatt
     5821 aaaaaactgg caagccatca tggcgagata taattatccc ttgattattt ggaattcgat
     5881 tgttattgct gtaatcagta cagttttagc tacagccagt tctacaatgg cgggttatgc
     5941 attagcaaag tatcgtttta tggggaataa actgttgttc aatacagtta tgctatctag
     6001 gatggtgcct ttttttacca cattgattcc tttattttac attatgagtc gtttaggatt
     6061 atcgaatact tatgcaggtg tgatcatccc ttcgattgcc agtacaactt cagtgtttat
     6121 gatgcgacaa tatagcagtc aatttcctac agcactgatg gaggctgcca gaatcgatgg
     6181 tgccaatgag tgggtgattt ttacgaaaat cgctgtaccg attttatcac ctacgattat
     6241 tactagtggt ttacttatct ttatgggaag ttggaatcaa tatctatttc cattagtcat
     6301 gctttcagat aaagataaat atacggttcc cttaattatc aaaaatatga cggtttctag
     6361 tacaggtgag ccaattaatt atggcgcaat tatgctaatt ttagcaactt cagtgatccc
     6421 aatggtcttg atttatgtgt gggcgcagtc taaatttaag gagaatgaca ttggagctgc
     6481 aaataagtaa aaaaaataac gaatgttaac catctagttc gttctaggag caactccaca
     6541 ttcataaaaa ttaaaagaat ggcgggatat ccatgaatga acagacgaag gaatggtcaa
     6601 gaaaaatttt gaatgtagct tctgaaaaga tagatgcgga attagacaga atggggaata
     6661 aaattccata tatttcttac aacggaaaat atacagacgt aataaaggac agaggaatcg
     6721 actggtggac caatggtttt tggagtggta ttttatggca gctctttcac aagacaaaac
     6781 gagaaaagtt caaagaggca gcaatacaac aagaacaatt attagaatct gccctgtacc
     6841 atttttcaga cgttcatcat gatgtaggtt ttttattctt accatcggct attgcacatt
     6901 atcaacagtc tcaaaacaaa catagtcgac aactagggtt acatgcagct acggtacttg
     6961 ctggacgcta taatatttca gggaactatt tagtagcatg gaatgaccat aaacctggtt
     7021 ggatgattat tgatagtatg atgaatttac cattactgta ttgggcttcg agggaaactg
     7081 gtgatccgag gttctctaaa attgctcggc agcatgcaga tacaacagct aagtatctag
     7141 tgaggaaaga tggatcaacc gggcatatcg cagaatttga tgcagaatca gggaaatata
     7201 ttcggttact tggcgggcaa ggatacaatg agaattcttg ttggagcaga ggaaatgcat
     7261 gggctttata tggattcgcg ttaagttatc gttacacaaa aaatccgtac tatctagcta
     7321 tagcagaaaa gatagcagta aattttattc agaaaatcga aaaaactggt tatattcctc
     7381 tcattgattt tgctgtaaaa gattcgaata ttgataccag tgctggagta tgcgctgcat
     7441 gcggtcttct ggaattagca ggatatgttt cctctgtacg atcaaaaatg tatcatgaag
     7501 ctgctatcaa tatactccaa aagacagaaa agcactatgc aaattggaat atggatgaag
     7561 atgggatcat tggaggcgga gcagaagcat accatagaga actgacttat aatgtgccac
     7621 tgatctattc tgattatttt tttattgaag gcttacttcg attagaacag tcggaattag
     7681 ctatttggta gtaaaaagag gagaaaatac aatgaaacat ccagtaccac tattaaggac
     7741 cagacaggat tactttgact tatttaaaaa gataaatcaa ccactccagc cgttttatcg
     7801 agcaagtggc gcacaaattg attatataca tcaaggggta ggatatggaa ataaaattgc
     7861 aggaatggaa ggattctctc gggttttatg gggagcgggg cccgcaattg accaattaga
     7921 tgaacgatgg ttgaaattaa ttctatcagg aataaaagct ggaacagatc cttcccatcc
     7981 cgattattgg ggagaaatac atgaaagaga ccagcgtatg gtagaaatgc ccgcaatgtt
     8041 attagcgtta tatcatcatg aacaattatt atggaaaaaa ttagatacag gagaaaaaag
     8101 ccagattatt cattggttct cgcaaatttt tgaatatgaa tgtgcagatg gtaattggca
     8161 attttttaag ataattgtcg gaacaattat ccaaaaattt ggttatccag ttaaagaatc
     8221 agcactgaaa gaagcatttg ataatataga aaattgttat cttcaaaatg gctggtatcg
     8281 agacagttca agaggtcgcc aagattatta taatcctttt gcatttcatt attatggttt
     8341 gatttatagc gtactagaac cagaaaagcc cattagccag atatataaga accgagctat
     8401 tgatttttct agagactata ttcatttttt tgcggaagat ggtgcaaatg tgccttttgg
     8461 tcgaagcatg atttatcgtt ttgcagtttc aagcttttgg tcagctatgt tgtgggccga
     8521 tcttttgcct tttaaattag gtgaaatcaa ggggttagtc aatcggaata taagatggtg
     8581 gatgcaaaaa gaaatatttg atgaaaaagg tatcctgaat attggttata cctaccctca
     8641 attgacattg actgaaccct ataatagtac gttatctcca ttatggctga ataagatttt
     8701 tttattgttg gcgctacctg aagaccatga gtattggacg gctaaagaaa tgcctatgcc
     8761 tattcgtgaa ggaactaaac ttctttcaga agccaatttg ctcatacaac atgataatgg
     8821 acatacattt tttttgaatg cgggacaatt agggccaaat ttccatacat tgaccaatga
     8881 aaaatattta aaatttgctt attctagtca atttggtttt tcgataccgc gagcgaatca
     8941 attgaaagca gaaatggcaa tggattcaat gctagggatt cagcgagcgg acacatatat
     9001 cacgaccagt tttaagaatg agagcatcaa aacctatgga cagttcatcg ttcgaaataa
     9061 tgtacgagac attgtatgta agaaagaggt acttgcctct acatgggaac ctactttaca
     9121 ggcaaaaatc agaacttggc ttattccttt tgaaggctgg cagattcgcg tacacaaagt
     9181 agaattagat acagaatatg ttctatatga gacagggttt gctattgaat gttctcctga
     9241 gaaagaagga ataatcatag aagaagacaa ggaaaactat agggtgagtg aagctggctt
     9301 ttcaggtatt atctgtttaa gtgacgatac tattaaacga aaatccacag ctattatggg
     9361 tttgcccaat acaaacttga tgacatggga aaacactttt attcctggat tagagggtac
     9421 atttggaaaa ggaacccatt ggctcggaac aggagtggtt gctcatcaag atcgggatta
     9481 tagtttagaa gtatggaaca atcgtcccaa aattgatttt gatgggacaa ccgggctaac
     9541 tatccaaaat aaaaacaata aaaaaagact aaaattatgc taaaggctgg tggggaaatg
     9601 cttgataaag tgatgtttga agagataata catccagaaa aatggataaa tgaatgttat
     9661 aaagaattaa tggaaaaaga atggaataat gagatcgagc gttgtcgcag acacacggag
     9721 aggttaatca aacgtatcag taacagtcag tatcaattcc caagcgagtg tcctataaaa
     9781 ggacgttatc cattaagaga caatattggt tggacagaag gattttggac aggaattaat
     9841 tggttgttat ttgagaagac gggtgatcct ttatttcaac aaacagctga aaaagcagat
     9901 atagatttta tccacaggat tgactgtcaa attatcacta atcatcacga tcttggtttt
     9961 ttgtattctt tatcaaccgt agcagattat aaaataacgg gaaatcgaaa aatgaaggaa
    10021 gcttcaattg atgctgcaga aacattactt cagcgttttt gggagaaggc aggagttata
    10081 caagcttggg gagatcctag tgatccttca caacaaggta gaatgatcat ggattgttgc
    10141 atgaatcttc cattattgta ttgggcgagt gaagaaactg gaaatcaaaa atataaaaaa
    10201 gctgctcagg ctcatattca aaatgcagca gattatttag taagagaaga tggttcaact
    10261 tatcatacat tttatatgga tgtcaaagat ggaaaaccaa gatatggaag tacgcatcaa
    10321 ggttatagtg ataattcctg ttgggctaga ggccaagcat gggggattta cggttttgca
    10381 ttaagttatc gttatttgaa agaacaaaaa tattttgatt taagcaaaat attagccaat
    10441 tattttatga atcggttgcc agaagatacc atttgttatt gggatttgat ctttgatgat
    10501 tctagcagag aagaaaaaga tacatctagt atggtgatca tggtttgtgg attattagaa
    10561 ttaatagaat caggtctatt agacaaaaca gaaaagaact tataccagtc tgctgtttgg
    10621 aaaattatgc gacaattgaa agaaagatat gctacaacgg aagaagaaga tggcttttta
    10681 acacagtctg tttataataa gcccaaaaat aatggggtaa atacaccttg tatttggggt
    10741 gattactatt atttagaagc tttagtgaga atgactagta attggtcttc ttattggtga
    10801 aaaaggtaaa tatggaattt ttgtttgata aaataaaaaa agaaaaacca aaacagcatg
    10861 aatggtttct gttttggttt tttcgttaat tttcatattc ttctaatt
//
LOCUS       DACCQI010000096        10673 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCQI010000096 DACCQI010000000
VERSION     DACCQI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.100"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IVO76_002154"
     CDS             complement(172..1590)
                     /gene="nhaC"
                     /locus_tag="IVO76_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7430095.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(1993..3216)
                     /locus_tag="IVO76_002155"
     CDS             complement(1993..3216)
                     /locus_tag="IVO76_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7430096.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(3247..4086)
                     /locus_tag="IVO76_002156"
     CDS             complement(3247..4086)
                     /locus_tag="IVO76_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7430097.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(4103..4783)
                     /locus_tag="IVO76_002157"
     CDS             complement(4103..4783)
                     /locus_tag="IVO76_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7430098.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(4785..5858)
                     /locus_tag="IVO76_002158"
     CDS             complement(4785..5858)
                     /locus_tag="IVO76_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7430099.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(6505..7176)
                     /locus_tag="IVO76_002159"
     CDS             complement(6505..7176)
                     /locus_tag="IVO76_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7430100.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IVO76_002160"
     CDS             complement(7263..9941)
                     /gene="mgtA"
                     /locus_tag="IVO76_002160"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7430101.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            10600..>10673
                     /locus_tag="IVO76_002161"
     CDS             10600..>10673
                     /locus_tag="IVO76_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7430102.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQR"
BASE COUNT         3082 a         2325 c         1808 g         3458 t
ORIGIN      
        1 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
       61 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
      121 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
      181 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
      241 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
      301 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
      361 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
      421 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
      481 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
      541 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
      601 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
      661 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
      721 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
      781 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
      841 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
      901 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
      961 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
     1021 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
     1081 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
     1141 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
     1201 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
     1261 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
     1321 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
     1381 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
     1441 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
     1501 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
     1561 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
     1621 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
     1681 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
     1741 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
     1801 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
     1861 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
     1921 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
     1981 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
     2041 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
     2101 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
     2161 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
     2221 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
     2281 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
     2341 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
     2401 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
     2461 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
     2521 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
     2581 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
     2641 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
     2701 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
     2761 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
     2821 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
     2881 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
     2941 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
     3001 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
     3061 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
     3121 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
     3181 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
     3241 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
     3301 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
     3361 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
     3421 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
     3481 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
     3541 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
     3601 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
     3661 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
     3721 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
     3781 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
     3841 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
     3901 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
     3961 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
     4021 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
     4081 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
     4141 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
     4201 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
     4261 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
     4321 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
     4381 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
     4441 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
     4501 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
     4561 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
     4621 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
     4681 atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa
     4741 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
     4801 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
     4861 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
     4921 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
     4981 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
     5041 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
     5101 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
     5161 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
     5221 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
     5281 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
     5341 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
     5401 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
     5461 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
     5521 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
     5581 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
     5641 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
     5701 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
     5761 acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
     5821 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
     5881 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
     5941 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
     6001 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
     6061 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
     6121 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
     6181 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
     6241 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
     6301 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
     6361 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
     6421 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
     6481 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
     6541 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
     6601 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
     6661 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
     6721 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
     6781 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
     6841 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
     6901 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
     6961 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
     7021 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
     7081 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
     7141 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
     7201 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
     7261 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
     7321 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
     7381 cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta
     7441 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
     7501 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
     7561 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
     7621 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
     7681 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
     7741 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
     7801 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
     7861 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
     7921 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
     7981 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
     8041 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
     8101 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
     8161 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
     8221 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
     8281 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
     8341 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
     8401 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
     8461 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
     8521 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
     8581 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
     8641 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
     8701 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
     8761 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
     8821 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
     8881 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
     8941 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
     9001 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
     9061 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
     9121 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
     9181 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
     9241 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
     9301 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
     9361 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
     9421 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
     9481 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
     9541 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
     9601 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
     9661 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
     9721 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
     9781 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
     9841 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
     9901 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
     9961 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    10021 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    10081 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    10141 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    10201 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    10261 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    10321 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    10381 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    10441 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    10501 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    10561 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    10621 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttt
//
LOCUS       DACCQI010000097        10473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCQI010000097 DACCQI010000000
VERSION     DACCQI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.101"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(184..1128)
                     /locus_tag="IVO76_002162"
     CDS             complement(184..1128)
                     /locus_tag="IVO76_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7430103.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1262..2653)
                     /locus_tag="IVO76_002163"
     CDS             complement(1262..2653)
                     /locus_tag="IVO76_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAP7430104.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2779..3189
                     /gene="tagD"
                     /locus_tag="IVO76_002164"
     CDS             2779..3189
                     /gene="tagD"
                     /locus_tag="IVO76_002164"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP7430105.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3365..3778
                     /locus_tag="IVO76_002165"
     CDS             3365..3778
                     /locus_tag="IVO76_002165"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7430106.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3935..4192
                     /locus_tag="IVO76_002166"
     CDS             3935..4192
                     /locus_tag="IVO76_002166"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7430107.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4279..5517
                     /locus_tag="IVO76_002167"
     CDS             4279..5517
                     /locus_tag="IVO76_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP7430108.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDEI
                     RAYTRESLKQYDLVEVADEE"
     gene            5507..6247
                     /locus_tag="IVO76_002168"
     CDS             5507..6247
                     /locus_tag="IVO76_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7430109.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            6657..6821
                     /locus_tag="IVO76_002169"
                     /pseudo
     CDS             6657..6821
                     /locus_tag="IVO76_002169"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6888..8315)
                     /locus_tag="IVO76_002170"
     CDS             complement(6888..8315)
                     /locus_tag="IVO76_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7430110.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(8312..9454)
                     /locus_tag="IVO76_002171"
     CDS             complement(8312..9454)
                     /locus_tag="IVO76_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAP7430111.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            complement(9458..10453)
                     /locus_tag="IVO76_002172"
     CDS             complement(9458..10453)
                     /locus_tag="IVO76_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7430112.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
BASE COUNT         3611 a         1875 c         1597 g         3390 t
ORIGIN      
        1 tgatcattaa gtttggcttt tatgtcataa ttgaatttta taaagttctc tttttcatgt
       61 aaaatattta attatatatt tcctaaataa aaagtaaaac aagaattaaa tagttggtta
      121 tacaacttta attcttgttt tactttttag aattatttat ccaaagatgc aatcattttt
      181 tcatcaaatt tctgtcttta aataccgttc cattccgctc aatatatcaa atttaggctg
      241 atagcctaaa gaacgaattt ttgaaatatc cgctaaggaa tcacgaatat cccctgaacg
      301 ctccgcttca tatttaagcg ctagctctgt tcctaagatt tgatcaattg aatggatcaa
      361 ttcaagtagc gtagtgcttt ttcctgtacc gacattgaat tgctgtccta aagctttttc
      421 ttccttggca acaagtaaaa gtgcttgtat aacatcttct atataaacaa agtctctaga
      481 ctgactccca tcgccaaata aagtaaattc cgctttttct cctgccaatt gttttttgta
      541 acgatccact agaatcgaaa tgactccgct atatggagag tttggatttt gatttggacc
      601 ataaacgtta aaaaatcgga cagcactagt cggaacatca tataaatgac agtaatccaa
      661 aacatattgt tctgctgcaa atttatcgat agcatatggt gttaatggac ggatgactga
      721 ttcttctttt tttggcagtg ttggttcatc accataaact gcagctgatg aactaaatac
      781 taatcgtttt aaattagatt gatatttccg tgctaactca agcaataaaa gcgcactgtc
      841 aaaatttaca cgatgtgttt ctacaggccg ttctacagaa tctgccacac tagctacagc
      901 agctagatga aaaatatagg caaactgata ttctttcatg attctttcca tcaattgaga
      961 atctgataca tccccctcaa tgaaagtcac ttgtttatcc atatccaagt tttctttttt
     1021 tcccattgat aaatcgtcaa ctactacgat tttgttttct tttcccaaat aattcgctag
     1081 cgttgaaccg ataaaaccag ctccgccagt aatcaatata gtgtccatca aattcactcc
     1141 aattgcacat tttcgtatca aataatatta gtttaccatt tttatattgt aaaaactata
     1201 aaatttgttg aagttgggat aactagtttt cttttctact ttcatatacc taaaaatagt
     1261 ttcaattagt tactccttct tctttagata tagttttttg gatagaaccc agatatcccc
     1321 aaaaaataaa tgaacctatc gtactaacca aaatgagttc tgtgataaat aatgccgaaa
     1381 ctgctattgt aagaatcatt aaaatagcaa aaaagtgctt atcttttact agatttggtt
     1441 gtgtgaaaca ataacgcaag gctttaaagc attggataaa taagaaggtt aacaatgtca
     1501 atagacctaa tcctcctgta gcagccatca ctaagaaaat aaaattatga gcagttaaag
     1561 tatcttttcc cattaatgta tatggtagtt tatcatgtgc atattcaata tagtagcctg
     1621 gagaacctcc ggatatcggc ttactcttga atatttcaat tgcacttttc caaagttcaa
     1681 accttaaatt agaaacatcc ccgctatcag tgtcagggcg ttttaaatca actttattgc
     1741 tgctagttgt tcctgatgaa tcagatgtat ctttttttac ttctgtaata tgcggaacat
     1801 gtgcccatgc atgagcagtt atatctgtta cataatacat cgcaattata cttagcaatc
     1861 ctactactaa tgcagttagc catcttttta aagtattagt tttaagaaaa cgactactat
     1921 aacttgtaaa gaaactaaaa acaaaagttg ttattaataa acaaactaat gtggtgcgtg
     1981 aacccgataa agcaataaac gagaattgaa taaaaatatt aattacaaaa aatattttaa
     2041 atagaagatt taaatgcttg taagtgacca aataatatat agcaataatg atgacgatta
     2101 atgatataac cgcaccaaaa tttggatctg tatatacacc aaataaacgg ttgcctaacc
     2161 accctaaacg aactgggtaa tacttatatt ttattggaat ttttatgcca tattgaatga
     2221 caaaccagta taatgatact aaattagcta aaaaccatac caatgaaagt atccaggtaa
     2281 atactttgaa aaaaaagtta ctactatagt ctttgccaaa ctgataaatc ataaagaaaa
     2341 agattaattg ccacatgatt aacttaatat tttcgctata tccatatttt aaattcatta
     2401 aactgcttac tagcatcgcg ccaataaaag caattaatag ccaatcatat ttttttagat
     2461 ttttttctct aaatgcaata attgcatcta taatgatcaa gacaactccc gctattccag
     2521 caggccaaaa aagtaaagta tcatatctag taggtaatag taaatacggg cctattgctc
     2581 gtaacattaa aataaaacca aaataaataa aataccaacc tttcacatat tttaaaaatc
     2641 tttcattcaa catttttcct ccatccacct atttctcata aatatttctt atccttttta
     2701 ggataaactg gtcctttcaa ttctaacaaa aaaatgctaa actaacaatg tatattaatg
     2761 aatttcgaga ggtgaaagat gaaaagagta attacttacg gtacctttga tttgcttcat
     2821 tacgggcata tcaatttact gaaacgagca aaacaatacg gagactatct tattgttgcc
     2881 ctttctacag atgaattcaa ttggaatgcg aaacaaaaaa aatgttattt tagttatgaa
     2941 aaaagaaaac agttacttga agctattcgc tacgttgatt tagttattcc tgaagaaaat
     3001 tgggaacaaa aagtaaaaga tataaaagaa taccatgcag attatttcgt gatgggcgat
     3061 gattgggctg gacattttga tgaacttgaa caactaacag atgcacaagt gatttattta
     3121 ccaagaacac ctgagatctc tacaactcaa ataaagaacg aacttaattc tttaaatagt
     3181 gataaataat attttttaaa caataaaaga acaatatatc tggataagac gtaaaaaaat
     3241 ggctctataa tttctaggac tgatagaatt ttctgtcggt cctagatttt ttgtaaaaaa
     3301 aaacagacat aaaaaaattc cgatagaata gagtcgtcta aaacatcaat ctgaaaggaa
     3361 gtttttgata tccatttctc attctatcaa agagctcttc actattaaag ataaaaatat
     3421 tgaaattaat tcttttcaag aaacttttta taaggacaag aaagccatcg taatcgaagg
     3481 tccattagtt cgcactttta gacattgtct attttgtaaa gaatctgcaa agcagatcga
     3541 gaaaaacggc aagaagattt caatgattat cttaaatcgc agtgggaata aacaaaccta
     3601 tttgcgtctt agaaaacaac attaccactg ccaatcatgc gggaaatatt ttactgctca
     3661 tacgtatctt gtcgctcctt catgttttat ttcgaaacaa gttcattaca cgatattaga
     3721 agagttgacc gagcgtcaag cattaaagac aattgcgaaa cgttgtgatt ttcagtaacg
     3781 acagttcaac ggacattatc tgctctatcg gcaacgctgc gacagaagct agactattta
     3841 cctcattgtt tactattcga cgagtttcgc tctctttcaa ttttccatgg gaagttctcc
     3901 ttttcttgta tgaattgaga gacagggaag ctctttgaca ttttgtccag ccggagaaaa
     3961 aaaagatttg attgcttatt tcatgcgttt tacaagaaaa gctcatatgg gggttcgata
     4021 cattgtcaca gatatgaatg ccccttattt ttcattagtc aaagaaccct tcccaaatgc
     4081 acaaatcatc attgattgct ttcacattgg gcaacacttg aacaagtttt tcgatcgtgt
     4141 tcgaaaacgt gtgatgaagc agttgaatca aaaatgtcaa cggcaccttt aaaatgtagg
     4201 aaaacggcaa tttaaaattg ttggtttacc tacatttttg tatgctcttc ttatcccaaa
     4261 tgaaatggag gctcagggat gagaaaagac atacgcgaag gagtgatgat ctacgtgatc
     4321 aatgaaatca aacccaatta tgctgcacta gcaaaacaat acgactgcga ctaccgaaca
     4381 gttaagcatg cttacgaaga agcccaagtt aaagaatcaa aacctccaga acgtaagaag
     4441 cgtccaagta agttagatcc ctacagagaa atcatccaag acaaaatcaa agaccaatgc
     4501 agtgcttaca gcattttcaa gtttattgag cacaaaggat ttggaggaag ctacagttta
     4561 gtaaagacct attgtcgcca gattaagcag gcgaaagtac acaaggcaac cgtccgagtc
     4621 gaacatagcc ctgggctttc tgctcaggtt gactggaagg aagatatgcg actaatcagt
     4681 aatcagggcg aaatttttga gttcaatatc tttttgtacg tcttgccata ttccaagaaa
     4741 aaatatgtca cactaacttt cgaccgaaaa caagacacac tctttgagtg cttagatgat
     4801 gcgttttatc aaaccggtgg cgttcccaaa gaaatctggt tcgataatat gaagacagtc
     4861 gttgaccatt ctcgtagtca gttttccaaa ccgcatttca acaaacgttt ttatgctttc
     4921 agtaaggatg ccggcttcac accaattgcc tgtcggccat ttcgtccgca aacaaagggc
     4981 tgtgtcgaag ctttagccag aactgttgac cgcctacggg tctataatca tgaattttat
     5041 gatgccgttg agctgtgtca tttagtgaat aacctttgtg ttgagttaaa taacgaggtt
     5101 tctcaatcaa ctgatcaaac accagatgaa cgttggcaga cgaacgaaaa agagcacctg
     5161 catccatacc aaaaaaacct tttgaatccc tattttgaag actttattcg ccgtaaagtc
     5221 accaaggaag aaatggtcat tttccgtaaa tgcaggtact ctgtggcgcc tcgctatatc
     5281 ggtaaagaag tcgaaatcga actctccgat aaagaagacc aagtacaaat ctattataac
     5341 ggagaactga ttcggtcgca tccattaacg acaaaacagc tgaactacca cgaggaagac
     5401 tatttccaaa tcttgaaatc cgatgtcatg agtcacaaag aagacgatga aatcagagcg
     5461 tatactcgtg aaagtctcaa gcaatatgat ctggtggagg ttgccgatga agaatagcca
     5521 ttaccaagaa ctgatgacca acttagagac tcttggtttg aatcatatgc gggaatatgt
     5581 tcctaactac atcgaagtgg ccaatcgaga ggagctttct ttaacggaag ctcttctcga
     5641 gctgacacgc agagaagttg atcatcaaaa taagcaaaaa atgaatcgtg tggtggcacg
     5701 cgcccgcttt tccaagagaa ccagcctgga agagtttgat tttgccttcc agccaagtat
     5761 caataagcgc gaagtgctag acttaaggca catgggcttt gtggaaaaac atgagaactt
     5821 ggtttttatc ggcagtcctg gtgtaggcaa gacacatctg acgattgcat tgggaatcga
     5881 agcctgtcgt caaggctatc ggacactatt catcacctgt catgagctgc ttttgcgtct
     5941 gcgaacagcc tatgaaaaag gtaccctaga gcgtgttctc aaacgatacg ctcgctatga
     6001 cgtcttgatt attgatgaga ttggctatct tccgattcaa aaacaggaag ctgatttatt
     6061 ctttcagctt cttgccatgc gctatgaaca ccgctctacg atcattacga cgaacattgt
     6121 cttatctcgt tggggggagt tgttccaaag ttctgagatc gcagctgcca tccttgaccg
     6181 tttagtacat cacgtcaagg tgttcaagat cacaggcaag tcttatcgta tgagagacaa
     6241 gatctaaagt gtcgattgtc gacaattact attgccgaaa acctacattt ttatcctgcc
     6301 caaaacctac aattttaagc tgcccttgac atcaaaaaga cacttcacaa gctaagtgct
     6361 atcgacaatt gaaacctttg tataaattac ttctataatc agatgacgag ttggattata
     6421 caacattcaa gaaatggcag aattttcaat ggcgctattt aacagaaagt gaagtagttg
     6481 attgtctacc ctcaatctct tatgaactta aaatggctta gcagtattat caaaagctgc
     6541 ttaacaatta tcacaagaaa cagctagcta cttcgagaaa tgaccaaaga agttccctcg
     6601 aaacttcact atcttaaaaa gcctttttca tatctgaaaa aggcttttaa ctagcatttt
     6661 cgaaaaagta ttttaataca aaattagaga atcttcgcac acatatcaaa acattgaaac
     6721 gatttgctta tggattttgc agttttacca atatgcgctt gtggattttt ctaatcaacg
     6781 ggttaataag ctatgcatta aaagctagta aatcttacta actttgttga tcataaatga
     6841 ccttatcagc ccgatttgac aaagaactac ttttttacaa cattatttta ttttcgaata
     6901 atctttctta cttcatttcg tatatctgga aaaagaaaat aaacaaacgg aagataaata
     6961 atgaaaatca ttattgattt aattgccata tctaaaaatg aagaagagaa taattgtgga
     7021 agaaagtaat aggccgaagt agtcaaagta agattgacta taaaaaataa gacaacgttc
     7081 ttcccaaaga aataggatat ttttatttct tttcgtacaa aaaatactct aaagaaaaag
     7141 actaataatt ctactaccac gagtacgatt gtagccgcta cagcgccata agtaggcaca
     7201 aaagctacat ttccaataat acttacaatc gcaccaatga tcattggata agcatactcc
     7261 ttatcttttt ctatagcaat tgcaaattgg ttagcaaaaa ctccaccaat tggactaaca
     7321 actataatta gacaagaaat aagcatgttt attgtcatcg gtttgaaatt ctccccaaaa
     7381 aaccatggaa caaattcttt tgtattgata aacaccacac taaaaagaat taatgcaata
     7441 ataatcgtgt aatcaaaaga tttttttgtg taaactaaaa tattttctga ttttccctct
     7501 gcctgtgctt tagatatttt aggcaacata acaatagtaa ttgatgttaa gactgccaat
     7561 ataatacgag caattttttg agactgatca taataagata atgctgttgg atccacaaaa
     7621 tacccaacta tcgttttgtc taaagttgtg tacacttgca taaacaactg aggagtcaaa
     7681 ataataaaag ctggtttcca caactgttta aaaacattag agttaaagag tggtttacct
     7741 acttttccgt ggatactcaa ccagaaaact aaacttccta aaaagttagt aattgaagta
     7801 atgaacacat aaatccacaa atcatcttta gttgaaacaa aaaggattat taacaatacc
     7861 gatgataatt tgataactgt atttctaata accactttcg aaaattcttc aattccttga
     7921 aaaaaccaag aaatatctaa tgttagccct gcaataaata tcaattgaat taagtataaa
     7981 tttttatact ttacatcaaa aaaataaata taggctatat agagaaagga aacactcaag
     8041 cctattaaaa attgaagaaa gtaaatattc cagaattgta aagatagtgc ttttttatct
     8101 ttccttgatt tagcgatatt tcgaatacga aaaggattca ttcccacagc aatgacagtc
     8161 attaaaaatt gtgatattga gaaaacatac ccataaatcc ctaaaccagt tgaacctatt
     8221 ctacgactaa gtataggtat agtaagtaag ggcaaaacaa tcaaaaagac ttgatatatc
     8281 gcattataaa atatatcttt tactgttttt ttcactttat atccctctct aacaactcaa
     8341 gaaccttttc tgtacttgcc cctgtttcat aatttccaat ataatcaagt aactcttgag
     8401 aacttttcaa aagcttttct tgttggtttt taaaaacatc aacaaggcca taactatctt
     8461 gtacgcttat aattggtaac tcacttaact gtatataaaa acctctttct aagctatatt
     8521 ctttttcatc atagttaaaa agaataattg gtttttttgt tagcgcaaaa tcgaaaaaaa
     8581 tagatgaata atctgtgatt aaaacatcta tctcttcaaa aagcatttca agaggaatcc
     8641 catcattaat taaagtaatt tgatgttttt tcatatattc tatactttta gaatcagttg
     8701 ctaatttact atgaacatta ggatgaaatt tagtcattaa atttaattct tgttgttgct
     8761 ttttaaaaaa ttcacgtaag ttatccacac ctaaccattc tagttgcgta attccgttgt
     8821 cctcaatatt atttcgaaaa gttggagcaa ataagacatt gatttgtttc tttttcaagt
     8881 caaacacttg ctctttacta gaggctaatt catcgtttct aggagttcct acttctaata
     8941 ttttagaagg aacgatattg aatgctgaaa catacttctc agaactatag cgattaccag
     9001 ataataaata tgtaattgac tcactatctt ttttcgcata gcttaggtaa cgtttaggta
     9061 acttatcttt tgcatcaaat tcaatttttt ttaaaggcgt accgtgccat gtttgaaaat
     9121 aaaattgttt tttccttttt gcatacttaa tcgttttccg actatcgtct atccaatact
     9181 tagctgtata caaataaaag gcagctaaaa aagaatcgga atcaattctt ttaatataat
     9241 ctggtaaatc agttataatc gcttcttttt tgattaacca aataattctg taattcaatt
     9301 ttttttcatg aattttctct gaaataactc ttggatttcc agaataatta cgcccgccaa
     9361 aacttgaaaa tacaattaaa tttttttgaa taggtaatat tttaataaaa accgggaaaa
     9421 tattccttga aaaaaatttt tttatattct tcatctctta cctcacagat tctatcttaa
     9481 tcaaaaattt acttgctatc ttactacttg acatatagta aggcaagttt ttatttatta
     9541 ataaaaactt tactaatttt tttaattttg gtgaaaagtc gggatcaatt ttcacttctt
     9601 tttcaaagcg ttgtaaaata tttatagaac gtaaataatc ttctttattg gtatagttat
     9661 ttttaatcat tgtcatcatc atccttattg cttcatagac atagcgagtt ctaacattaa
     9721 ttgataattc cggataaaac ttatctatga atactagtat atcttcatgg acttcaaaag
     9781 aatcaaaaat tctaggattg aagcttcctc tcagtatact tttagggctt tggtaatagt
     9841 gatatttagc agatgaattg acagcaactt tttcggaatg atcgatcaac aaataagtag
     9901 tagccttatc ttccattaat ttacccagtg ggaatcttat attatcaaat aaatttctat
     9961 tgaataattt attatttgca gaatagccaa ttttattatt tataaataag tttttcattg
    10021 cttgttctct atcaaatatt tcccattttc tctcagcaat tgtatgttct tttagttgtt
    10081 ctaaacttga aatctttttt atttttagca ccgagatatc tgctccataa gtttgcgtac
    10141 caaaatatag taactcaaga tagtctggtt ctatccaatc atcgctgtca ataaagcaca
    10201 caaaatctcc tgttgcagaa tcaagaccag aattcctagc cgcacttaaa ccaccattca
    10261 cttgatgtat cacttttaat cgcatatctt tctttttata ttcttctgca atttccccac
    10321 ttcgatccgg ggaaccatca tcgactacta ttatttcaat atttgaatgt gtctgattgg
    10381 aaatagattc taagcagttt tctaaatagt tttctacgtt atatgcgggt actataacac
    10441 taattttatg cactctcttc acttcctaaa aat
//
LOCUS       DACCQI010000098         9957 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCQI010000098 DACCQI010000000
VERSION     DACCQI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.102"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(124..459)
                     /locus_tag="IVO76_002173"
     CDS             complement(124..459)
                     /locus_tag="IVO76_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430113.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(473..1507)
                     /locus_tag="IVO76_002174"
     CDS             complement(473..1507)
                     /locus_tag="IVO76_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7430114.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(1597..2277)
                     /locus_tag="IVO76_002175"
     CDS             complement(1597..2277)
                     /locus_tag="IVO76_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7430115.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(2561..3664)
                     /locus_tag="IVO76_002176"
     CDS             complement(2561..3664)
                     /locus_tag="IVO76_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7430116.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(4293..4763)
                     /locus_tag="IVO76_002177"
     CDS             complement(4293..4763)
                     /locus_tag="IVO76_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7430117.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            5093..6733
                     /locus_tag="IVO76_002178"
     CDS             5093..6733
                     /locus_tag="IVO76_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7430118.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            6887..8209
                     /locus_tag="IVO76_002179"
     CDS             6887..8209
                     /locus_tag="IVO76_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7430119.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(8277..8711)
                     /locus_tag="IVO76_002180"
     CDS             complement(8277..8711)
                     /locus_tag="IVO76_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7430120.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(8776..9612)
                     /locus_tag="IVO76_002181"
     CDS             complement(8776..9612)
                     /locus_tag="IVO76_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7430121.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
BASE COUNT         2931 a         2039 c         1782 g         3205 t
ORIGIN      
        1 tacgtgtatg gatcataaga aatggcagga ggcgttgctc caacagcttc tgccgaaggt
       61 gaaaagataa atatacttaa aacaaaaagc gaagtaaaaa agaaggagcg aaaaaatgaa
      121 aatttatttt gtagcaacat gttcatcctc cttttccttt cttctccata acaacaacca
      181 cgcaagaagc cataattcta taccaatgat caaataaaga aaacggatct tttcttcatt
      241 cgtctccggg aatacgccat gttgattaga ttctttataa atcggtagct gtgtctgctg
      301 ttttgatgcg ccattgactg aaccacttgg atgcggaact ggacttagat cataaaatgt
      361 aatcgtacct gaacttttgg tatctgcttg gactggctct tgccagctga agaacagcaa
      421 acttgcaata atccctccca ataagatcga tgttttcaac gaaatcacct catcatgttg
      481 tttctccttt tttctttttc ttgtggaccc acaaaaacag caaaatcaac cataacacta
      541 gaactgcagc aaccaatcct gcataaagcc atgggaaaga ttgttcttgt actgcaaaaa
      601 cggaattttc atttactttt tgtgcttttt cttttttgat cgtaaagttt tgtgtaaatc
      661 gccattcatt actgtcgcct gattttgcta tgatttctgc gcggtaatcg ccggcttttg
      721 ctttatcacc agctaaatca agattcaagt gaaaattcga atttggtgca actaaaaatt
      781 cattagacgt attttcaata taaagctgat tttcataaaa gatcttgctc gtaaatatca
      841 aattgctgat aatcctaggc tgaggatttt ggaaacgaag ctgaacagtt ggctttgcgt
      901 tgacctcgtc caaatctcct gataaaagtt tcagatccgg ttctatcgtc tcttgacttt
      961 cttgtaaaac gactgcgaga ttataagcaa tacgattttt gatttgtgct ccttctgtag
     1021 cattttctgt ttcagcttct tctttaggtg aaacatgaat acccgctaat acacgtccct
     1081 taaagctttt tgctgggatt ttcacttcta taggtatagt tgtttctgca tgtgctgata
     1141 cattgatttc ctttttcgca atgagcaaaa cgtcacggac atcaaacggc aatgtctcat
     1201 ctaacgttgg gtttttttga ctataatcga tcgttccacc atcactcgtt accgccggat
     1261 tgacagagac ctcaaatgtt cgttcttcat ctgaagaatt gctgataatt acttctagtg
     1321 tctgattttg ttccggtgcc actaataaat caaaataacc gattgcttta tctatttggt
     1381 tctctggaaa taccggtttg attccaaagt ctcctgctga atcctttgct tggctgattg
     1441 acggaaaaat gaaaaataga cttaaaatag aaaaaaacga aacaaacagt cgtttacttt
     1501 gattcattgt ccaccacatc ctttcaacaa caactgcggt aaaaaatcct tcacctatag
     1561 tattttcacc gcagtttgtt gaatttattt catactctat gctactgggc cagccacgag
     1621 gttccaagtc aaatcagccg tgaatgttcc agctgctgct ttcggcactt tcagtgtgat
     1681 atttgctgga tcataaacag aaagccatgt tcccagacct tgatcttttg ctgctgtaaa
     1741 tattggtgtc gctgcacttg ctttactcaa cccagttaca gtttgactag ttggtccagt
     1801 ggattcgtta tctgacgtac ttttaacagt ggatgttcca aaatctaatt cagcgccttg
     1861 caaaacttgt gaaccgttta caaaatctga aaccgaaact gtcacgttcc acccttgtgc
     1921 ttggccatct gcacctacac ctcgacgatc tgatacttgg agataaggtg tatcgttttt
     1981 tgaagcgtct acttgatacg tttttgttcc gctttcaatc tcgtgtgtac caaatggcaa
     2041 ttcaggaact acatccaatg ttaaagaacc tgtctggcca gttgctggat tttccggatc
     2101 tgctggctct actggttttg aaggatcagt agggttaaga ggctcaacgg tatttgttgg
     2161 ttcagtaaag gtaatatctc cttttgaagt cgcttgaccc tcagcaaaag ctggtgttgc
     2221 caagccgcct agaagaagtg ttgaaagtaa taatgtccca atcatgttct ttttcataat
     2281 aaatcactcc tttttcttag tgacctaagt atagtagaga cagtatgcaa aaaatcctca
     2341 tgaattcatc cgcttacttg aaattatgac cctttttgta taaaagagaa aaaaacaata
     2401 aaatcgttta catgaatatt cttccttttt tattaagtat gaaacaatat ttcaatttta
     2461 ttttttcgtt tcaaattatt ctattagtga catccttgaa ctataaaaat aggagtacgg
     2521 catcatctgc cgcactccta tttttttcgt catttatttc ttaaataaaa cgattctcac
     2581 gcaattttat ttttttgata tcttctactg tttgtgttcc agctaattgc atgaccattt
     2641 ccaattctgt tttaaagaaa tcaaacactt gatggactcc tgtacttcct cctaaagaca
     2701 atccataaat aacgggtctg ccaatagcaa caagatcggc acctgatgcg attgctttga
     2761 aaacatgttg tccacgtcgt acaccgctgt caaacacgat tggtacacgt ttatctactg
     2821 cttctgccac atattgcaac gaatcaaacg cagcaggccc cccatctaat tgacggccac
     2881 catggttaga tacccaaatt ccaccagcac cagattctaa tgatcgatag acatcttctt
     2941 ctgattgaac acctttcacg tagactggca aatcagaatg agcagcgata aattctacat
     3001 cttttggact aagtttttgt ttggaagatt tatatacagc atccatcgtt tggcctactc
     3061 ctgattgata agcctgaacg atcggcattg gcagcgggaa ggtaaacccg tttctgcggt
     3121 ctgtttcacg gtttccgcca actgttgcat ccgctgtcaa tacgattgct tttgctccat
     3181 tacgcttggc cacttcaaga atatccaaat tgattccatt atctttgctc atataaaatt
     3241 gaaaccattg cggtgctttc tccccgcctg cttctcttat ctcttctaaa gtacaagaag
     3301 cgtaagaact tgctgtatag atcgtcccaa aatctgctac accttttgca gaagctttct
     3361 ccgccttcac atgagctaat ccatgagcag caactggtgc catgatgatt ggtgcagtca
     3421 gcatttcttc gtcaaaatgt gtagttgtat cgggtaattc tacatctctt aatacatgag
     3481 gaataatcaa acgatgattg aatgcccgtt cattttcttg ataagtaaat aagtcgccag
     3541 caccactgct gatgtaacca taaccaccag ctggaatcac ttgtgaagcc gcgatttcta
     3601 aatcttccat attgataaaa tctactattc cttcatttgt acctgcttga tatgtttttt
     3661 ccatctcaat gccttctctc tataaaattt gaatgatgaa caatatgata gcgtttacac
     3721 tttttatatt actcttcgac attgatacag gcaaataatc tgtttacatt tcagccattc
     3781 cagatgtcac tactctggaa gaaatgattg taataaaaat agataaaaaa ccgatgggac
     3841 tttgacaaaa gtagtgtcaa agtccctttt tttgaataaa cggtgttcaa aagccatttt
     3901 ctcggtatta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3961 actactcgaa actaagcgac tttttcacaa cctctcgata aaacggtgtt caatcagctg
     4021 accctcggta ttagctcaaa aaatggcaaa atatgaggaa ctattttgcc atttgtctct
     4081 ctaatactca ggtcagaacg ttgacttcac agtctcttcc ttaaacggtg ttcaaaaact
     4141 tgcatctgcg gtaataagcc caaccaaacg aaaaatagga ggagctattt ttgtttggct
     4201 gttcttatta cttgatgcag ggcaggtttt tcacgacctc tgattcacgg tgtttctttt
     4261 tcatcaacat ttttacgaaa tactgtggaa gactattgaa ataagtgtgg attgtttctt
     4321 tgtgtttcaa tcgtttcttg ccattcttca tttaattttg gattaccgtt ttcttccttg
     4381 aactcataag gtcctaattc aaaaatccca gacagtattt gattcacgat ttcatatagt
     4441 tcagtgtgat cagacacatg attcttgaag atcatctcat tatcaccacg tttgaacctc
     4501 acattcgggc tctcatgatg aacctctatg catttttctc ctttttttaa ctgacggact
     4561 tctgaaaaca tctcataaat atcttctcca gtaaaacctt cagcaagcgc cagactgtcc
     4621 acaagaccgc ctactgtcag ttcagagatt attgcttgat tggaatctaa ttttaatgta
     4681 tagcttgtcg cttctactac gccacatgca ttgcaaccgc cttctgatag ttcgcttttt
     4741 ctaatcaatt gaacttcttt cattttctct tcctcctctt aattttctac ttatgtattt
     4801 ttatcacctg acataggtga ctcctatcaa cgctatcatg tgcttttttc ctcagaaatc
     4861 ctatttatcc attccatcta tcccttattc ttctactttt ctttctctag catataagag
     4921 atacgcatag tattcaagca aggattgtcg atataaaaag aacaaatact gctattttga
     4981 tttcttttgg ctatattaca tataaataaa atttatcata cctgctcttt ttgactatat
     5041 catcatctga cttctatact tcgtttgtat taaaacttag gaggaatcat tcatgcttac
     5101 agggttaaac ttattaaata atccttttct aaataaagga acagctttta caaaagaaga
     5161 acgggcaaaa tatggaatca ctggtatgct tccaagtact gtccaaacac tggaacaaca
     5221 atctgttcaa gcttatggtc aatacttaag caaacaaaca gatttggaaa aacgtatctt
     5281 cttaatgaat ctattcaata cgaatcggac attgttttat aaattgatgg gcgaacattt
     5341 agttgaattc atgcctgtcg tttatgatcc tgtcgttgcg gattcaatcg aacaatacaa
     5401 cgaaattttc ttgaaaccgc aagatgctgc atttctttca atcgacgaac cagaaaacat
     5461 caaagcaagt ttgaagaacg cggcagatgg acgagacatt cgtttgattg ttgtgactga
     5521 tgcagaagga atcttaggta tgggcgactg gggtgtgaac ggtgttgaca tcgctatcgg
     5581 caaattgatg gtctatactg ctgctgctgg tattaatcca gcacaagttt tgcctgtatc
     5641 gatcgacgct ggtactaata atgaagaatt attgaataat cccttatact taggcaatcg
     5701 tcatgctcgt gtagaaggcg aaacttacta tgaattcatc gatcaattcg ttcaatcagc
     5761 taccgaatta ttcccagaat tgttattgca ttgggaagat tttggacgag gaaatgctgc
     5821 agcgatctta gaaaaatacg aagatcaaat cactactttc aacgacgata tccaaggtac
     5881 aggaattgtt gtcctagcag gtgtattagg cggattgaat atatccggtg aacaattgaa
     5941 agatcaaata attttgacat ttggtgctgg tactgctggt gtgggtatcg caaacatcct
     6001 tttagatgag atgatccgtc aaggtgtacc tgaagaagaa gcacgccaac atttctatca
     6061 agtagataaa caaggtttat tgtttgaaga tacagaagga ttgactcctg gtcaaatccc
     6121 attcgctcgc aaacgctcag atttcactaa tgctgatgaa ttgacaaact tagaagcagt
     6181 tgtaaaagcg atccatccga caatcatgat tggtacttct acccaacctg gagcattcac
     6241 tgaagaaatc gtgaaagaaa tggctgctca tacaccacgt ccgatcatca tgcctttgtc
     6301 taatccaaca aaattggcag aagcaaaagc aaaagattta atagaatgga cggacggaaa
     6361 agcattagtt ggaacaggta tccctgctgc tgatgttgaa tataatggtg taacttacca
     6421 aatcggtcaa gcaaataatg ctttgatgta cccaggttta ggacttggtt tgattgcttc
     6481 aacagctaca cgagtaaatg ctgaaatcat ttctcaagct agtcgagcat taggcggaat
     6541 cgttgatgta acaaaaccag gagcagctat cttgcctcca gtagcaaaaa tcactgaatt
     6601 ttctcaaacg attgctgaaa ctgtcgcaaa atcagttgtc gcacaaaact tgaatcgtga
     6661 agaaatcacg gatatcaaag aagcagttga atctgctaaa tgggttcctg aatataaatc
     6721 tttagaagat taagatcgtt atagtaaaag aacatacaga aactttgtat gacgtctaaa
     6781 aagaaaaaag cgactgcggc aaaacagcgg ccagccaact cctgctcttt tgtcgctttt
     6841 ctttggcttt tttcatatga tacagaataa gaaaggatct gtaaaaatgg cacaaaaaac
     6901 aacaactgca actgcagttg aggaaaagaa aaaagaaggt ttttggtcca caaaaatcac
     6961 cggcgtaagt ttgccacttt atttgatcat ggtccttgtc ctgatcattg caatggcttt
     7021 agggaaatta ccgacgaata tgattggacc aatcgcagtt ttggttattt tcggtaattt
     7081 atttcatttt atcgggacaa aaatccctat cgtcaaaagt tatttgggtg gtggttccgt
     7141 ttttgcgatc ttcgcttctg ctgcgattgc tacatttggg atcttacctg aatatgtagt
     7201 gaaatccaca gaaaactttg tcagcaatat gggcttttgg gacttttata ttgctgcctt
     7261 gattactggt agcatccttg gtatgaaccg taatctattg atgaaagctt ctgttcgttt
     7321 tattcccgtt gcacttattt caatggtcgt ctcctttttt gccgtaggac tcgtgggcat
     7381 gcttatcgga aatggttttg caaattccgt tttatacatc tcattacctg cgatggctgg
     7441 tggtgttgga gccggagccg ttcctttatc aacgatttat gcaaatgttt taggtacgga
     7501 tgcttcttct atcatttccc gcctgattcc tgcgacagca atgacaaatg tcttagctat
     7561 catcggtgca gcattagttg ctcgtgctgg acaatcaatg ccaaaattca acggcaatgg
     7621 taaattaatg gaaaaaggcg atcttggcga tggaaaacct cgaactgtga aaccttccat
     7681 tccacagtta ggtgttggtt tgatggtatc attgactttc ttcatcttag gacaaattgg
     7741 taactatttt gttcctaaag tccatgctta cgcatttatg attattatcg ttgtcatctg
     7801 taaaatcgcc aacctattac cagagtatta tgaagatgca gctatcatgt tcaataactt
     7861 gattgtcaaa aacttgacag cagctgttct tgccggtatc ggtattgcgt tgctgaatct
     7921 gaatgtatta gcttctgctt tgacatggca atttgttgtc ttatgtctga caagtgttat
     7981 cgcgatttca gttgtttcag gatttgtcgg tcgtttgttt ggtttatatc caattgaatc
     8041 aactattact gctggcttat gcaacaactc gatgggcgga acaggaaatg tagccgtctt
     8101 atctgcagct aatcggatgg aacttattgc ttttgctcaa atgggcaatc gacttggtgg
     8161 tgccatcgtt ttgatcatct ctggtttctt gatgcaatta ctttcttagt caaaaaaaca
     8221 aaaatacctg atcatcaagt atcggcttgg tgatcgggta tttttttgat aaagatttac
     8281 aggattttca aaaatctttt ttgcatttta tcggaatgat aaccgatgtg ttcataaaat
     8341 tggtgtgctt ctactctgta ttctgcagaa ttaagcctta ttgtcgttat tcctcgtttt
     8401 ttcgcttctt cttctacgcc agtcatcaaa gcctttccga tgcctctttt ttcagcatat
     8461 ttagacacag caagtgccat aatattgaac attggatcgc taaaaatcga ttcataggac
     8521 tccgcttgta catagccagc tgctttttta gaagtaggat cttcataaac caagaaaaaa
     8581 tttttctctg gctccttcag cagtttcttc atttgtctca ccgtaacggt taatggtata
     8641 tcatacccta attgatccgc attgatttca cgtagtgctt ctgcatcttc taaacgcatt
     8701 tcccgtaaca tccaaatcac cagccaattt ctgatttttt aaaatgaatt tttttatcat
     8761 agtatactct ttttttcatt tagaacaata tgtttcagtg attttgactt gcgttagtct
     8821 agattctcct aatgtttgta agatgacaaa ggcattgtaa caattgacca aaagaaccac
     8881 gaggacaatt acttgaaaaa taaaggtcac tttttttgga gacaaaaccc tgctaaatct
     8941 tgctccccac aaacctccaa taacagcagc ggggataaca taaaacaaca tagataaatc
     9001 aaagcgcata aagctacttg ttaaagcaat cgttaccaat tttgccagtt gagaaaagaa
     9061 aatcgtagat agagaatata gtgttgcttc ttttattggc aatgcaaaca tcagcatcaa
     9121 caaagaaaca ttgactggtc caccaccaat acctaaaaaa ctagctaaaa agcccagaac
     9181 ccccccacat ataaggtacc aagtccatga cgtcaaatga aattttggct gatgatgctt
     9241 ggtgtagaag aacgcaaaaa ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac
     9301 atgcttctcg ttttcaaata actgtaaaaa aacttcgaat acaatgttgc cgactactcc
     9361 tccaacaaca gctccactag atacccaacc gacaatctgc caattcagtg atttcccgct
     9421 tgatacttgt cgcacagtcg aaacaatgga catcgtgaat accgcaactg ttgagtaaaa
     9481 agaaatccct gcaacagaat gggcgccgat aagatcaaga atcggtttga tcaacacgcc
     9541 tccacccatc ccggaaatag cgccaactgt gttcgccaga acgatcacca caaaataaat
     9601 cagaccaatc atcttgtatt tcctttctct tgtttatatc ttttagcgat tgttactctt
     9661 ttcttttatt atacagtaaa agaaacaaac gaggcttcga aagcttttct actttttaag
     9721 gaaatttagt gctaaacgaa aagaggttgt gaaaaagctg tcctgcatca agtaataaga
     9781 acagccaaac aaaaatagct cctcataatt ttcgtttggt tgaacttatt accgcagatg
     9841 caagcttttg aacaccgttt aagaaagagg tcgtgagcct gccgttcagc tccgagtcac
     9901 aaggaacaat ttcaaaaaac agcttctcat gtttgttgaa tattgtgact tgtgacc
//
LOCUS       DACCQI010000099         9940 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCQI010000099 DACCQI010000000
VERSION     DACCQI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.103"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(27..683)
                     /locus_tag="IVO76_002182"
     CDS             complement(27..683)
                     /locus_tag="IVO76_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7430122.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            876..1154
                     /locus_tag="IVO76_002183"
     CDS             876..1154
                     /locus_tag="IVO76_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430123.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1173..2381
                     /locus_tag="IVO76_002184"
     CDS             1173..2381
                     /locus_tag="IVO76_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7430124.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2529..2894)
                     /locus_tag="IVO76_002185"
     CDS             complement(2529..2894)
                     /locus_tag="IVO76_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7430125.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3041..3814)
                     /locus_tag="IVO76_002186"
     CDS             complement(3041..3814)
                     /locus_tag="IVO76_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7430126.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(3988..4848)
                     /locus_tag="IVO76_002187"
     CDS             complement(3988..4848)
                     /locus_tag="IVO76_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7430127.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4848..6266)
                     /locus_tag="IVO76_002188"
     CDS             complement(4848..6266)
                     /locus_tag="IVO76_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7430128.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6323..6772)
                     /locus_tag="IVO76_002189"
     CDS             complement(6323..6772)
                     /locus_tag="IVO76_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7430129.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7217..7480)
                     /locus_tag="IVO76_002190"
     CDS             complement(7217..7480)
                     /locus_tag="IVO76_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7430130.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7579..8874)
                     /locus_tag="IVO76_002191"
     CDS             complement(7579..8874)
                     /locus_tag="IVO76_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7430131.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8871..>9940)
                     /locus_tag="IVO76_002192"
     CDS             complement(8871..>9940)
                     /locus_tag="IVO76_002192"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7430132.1"
                     /translation="NILEIDPREVVSIPMPHGKINSLRASYYFMGTLLGKFGEAVVGL
                     PGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFMDVVSVGATIN
                     VMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIEGVETLHGCRH
                     FMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMKISEDEIEVYP
                     SKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVNHVPELARMGA
                     DITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYNVDYILRGYDH
                     IIEKLTALGASIEMIEEVGAE"
BASE COUNT         2783 a         2105 c         1720 g         3332 t
ORIGIN      
        1 cgctgatttc acaacctctt tgttcatcaa aagataatat tgtcttctaa caagtccata
       61 ttcaaaataa cgatatgatg gtctttaacg gtgataatac catctttttt cagttgtttt
      121 aaaattctgt ttacactgct agcagttgag atgccacaaa atttggcgat ttcttcattc
      181 gtgatcgaaa aatcgatcag catcccttct tctgtccgca caccgaataa atcatataat
      241 tcgtaaagtt gcgtacaaac agctcccagc tttccgttta atagcatttg ctgcattttt
      301 ttcatcgaat aaagcagacg atagcggtaa tactctttta catagtgttg tagctttggg
      361 tcttggttga tattttgcca aaagcgcaca cgattgattc gataaaactc tgcatattct
      421 gattcaactc gaatattata cggcgaatcg atgaattgtg aatattcgtc ttttaaaaga
      481 gagacgattt caagatcttt gatatagcga aggttaaatt ctctcccatc atttgaaata
      541 acacttgctt ttaccgttcc ctctttcaag atatacgtat gggaatcttc catcccttcg
      601 aatgtcaaat atttatgata tcctttgcgt ataatcggaa aatcattttt atccaaatat
      661 tcctttaaga ctctactatc caatgatgct cccccatcca aactttcatc taaaaagatc
      721 atacagtact ttaaaacttt ttaaagtcca aaggtatttt actaatgata ttaacatttg
      781 ttaagtacct ttttccatca cttatgcgat ttttttcctt ttttttattc gttatactgc
      841 agaagtaaaa ttttttaaga atagagggaa aaaacatgaa ttactggatg aacacaatca
      901 ttaatcgatt agaaacagca tatcaaacaa gatttgatat gaaagcttct ttagtctttt
      961 taaatgatgc gtatcaaaac tcgatcgaat tgatcaaagc agtggatgag aatccaacaa
     1021 acgaatgcga agaattttta aatctgttta tgtctacaag agatttattt attcgccaac
     1081 ttgttgatcg ctatccatcc aattatcacg atgtagaagt tcaaatacaa aaattaaaag
     1141 catattcagc ttaaggggaa tgtataacat acatggaaaa gaaattattt aataaatatt
     1201 ttattggaat cacattgatc aattttattg tctacctgat ttattatctg ttgatggtca
     1261 tcatggcagt cattgcgcaa gacaccttac acgccagctt gagccaagca ggttttgctt
     1321 ctgggatcta tatcatcgga acattgctgg cacgcttatt catggggaaa cagttagaac
     1381 tatttggtcg gaagtttacc ttaagaggcg gcgcgatctt ctatctcctt tcaacattag
     1441 catatcttta tacgccaact atcggggcat tgtatttagt tcgattcttg aatggattcg
     1501 gctacggtac agtttctaca gctacaaatg ctatcgttac tgcatatatc cctgcatcta
     1561 aaaaagggga aggcatcaat tattatggat taagtactag tttagctgcc gctgttgggc
     1621 catttttagg aatgattttg ctgaatatga ctgattttcg tttcattatc tggttttcaa
     1681 ttgtactggt cttctttgtt acgattgcct gccttgtctt tcctgtaaaa aatattccat
     1741 tatctgaaga acaaaaaata tctcttcgct catggaatat caatagtttt attgaaaaga
     1801 aagcactttt cattacagga attgcctttt tgatgggtct ttcttattca agtgttttat
     1861 catttttatc ttcttataca aaagtaatcc acttagttgc tgctgggtca tttttcttcg
     1921 tcgtatatgc tttggtcatc acatttacac gtccattgac tggaaggatt ttcgatgtca
     1981 aaggagaaca atacgtgatg tatcctagct atttattctt aacagcagga ttatttttac
     2041 tgagtgtaac gacgaatagt gtgacattgc ttatctctgg agcattagtc ggcctaggtt
     2101 atggtacgtt tatgtcaaat ggacaggctg tttgtttaaa aatcgtccgc gaacatcgaa
     2161 tcagtattgc tttatctacc tactttattg gtttagacct aggcttaggc gttggtcctt
     2221 atttgatggg gatgctaaaa tctcttactt catttagagg attgtatgtg attgccggag
     2281 tcattccatt gatatgtaca gcactctatc ttgttaatgg ccgaaaacat agtcatgaag
     2341 cagagggcga attgattgaa tcattgaaaa atgaaatttg atcttgatga atagataaaa
     2401 aaaaccgttc agatctgaaa ataatctgag cggttttttg agtaaaaata gtttatgatt
     2461 attcttaatt aaacatctat ttatttttaa agctttcatt tatcgataag aatagtttaa
     2521 ctgttgattt attccatttt ccgttcatct ggtacgatgc cttgacttag caaccacttg
     2581 tggaagaacc attctgccac accgatcaat agagcaggaa tcaatacttt catccaaata
     2641 tcgactgtat cattcataaa atatagatag agccacaaga taactgcagc tgaaccagca
     2701 tctgcgatcg tcgctgcgat gttccctact ttacgtaaga tcaacagatc ccctagcaca
     2761 tagatcatga ctacaataat taccccaaca atcgttgaat ccataaagct aacaccgtat
     2821 aaacctgtca gcacgaccca cagaatcgcc cagatcatga ttgccttcac taccaacgcc
     2881 ttcacatgtt tcattttctg cactcctttc tcctggataa aaaagtaaga ttatctaaca
     2941 agataacctt ttcattatta taccatcggc tgagagaagc gagcaaagat aaggtcctat
     3001 ctaaaaatga ggaagccact tagaacattc acgtaataat ttatccgtac agacattcga
     3061 tatggtgttc ctttactttc gcatactgtt cttttgtcag ttctttactt gtcaccagat
     3121 aatcgatttc atgaaaagca atggcttgat gcatgaattt atgtgcaatt ttttgctgtg
     3181 taactaaaac ggcaagccgg tctgattgtt ttctaaaagc ttttttgacc attgcctctc
     3241 cttcatgagt ttctgttact ccttggctga aatctagccc gcgacaactc attatcgcta
     3301 aatccgcata atatgacaat gcatcgttat aggcttttga accgttgatt gttgctcgtt
     3361 tcggaacaat tgcccctccc aatacagata cttttgccgt tgtatattca ctcaacatag
     3421 cagctgtgac gatgccattc gtaatgatct gcaaaccatc aaatcttgct aggtttctga
     3481 ctaagtgtaa agaggttgta ctggaatcca agaataagac catatcatct cgaatcaaag
     3541 aagaagctgt atctgcaatc ttacgttttt ctgtttcttc ttctaatttg gtgatcttca
     3601 tcaggtaagg cacgtcaatt tttcgattgt caataagcat ggcgccaccg cgaactctac
     3661 ggatcaatcc acttttttcc agaaaaacaa ggtcacgttt aatcgtggct tcgctataat
     3721 ttactaactc gatcaaatcg gtgactcttg caaattggtt gttttttaga taattcacaa
     3781 tctcctgctg tctattctcc acttcattca acataaaatc accccattta ctttatatag
     3841 taacaggaaa aaagaggttg tgacagaagt gattggcttc gagaaataag aagaaatttt
     3901 tcgaaaaaac tacttctgaa catcgtgtgt ggttaagagg gtatgacaag actattgtca
     3961 taccctcttg accacacatt tttattttta gtataaacta gccttgccta ctgtgttcaa
     4021 aatttccatt ttatgtttga ccacttcttg taccttttta ttagctgctg gataaattgc
     4081 attcggctcg tacatcgatg gattttcact caatatttct ctcaaagcat tgaaaaagac
     4141 gcttttaaga tctgagctca aattcacttt ggcaactccg tattttactg cttcgctgac
     4201 ttctgcgtcc ggattacctg aaccgccatg taaaacaaat ggaagatcga tttgttggtt
     4261 caattctttc agtaagtcga tatttaattt tggagttctg tttttcggat acaagccatg
     4321 cgctgttcca attgctacag caagtgtatc gattcctgtt tctttcacaa aaatcttcgc
     4381 ttgatcagga tctgtataaa tgattgtttc agcacctcct tcagcagaac cattattccc
     4441 aatcgttcca agctcagcct ccactgagac atttaccgca tgagctaacg caaccacttt
     4501 tttcgtaatc tgtacattgt cgtcgaatga tttcaaagac gcatcgatca tcacggaaga
     4561 atagccgttt cgaatcgccc gtaagacatc ttctatcgta ccgccatgat ccaagtggat
     4621 aacgatcggc agtttacttg tataagcata ggctcttact gccgcaacaa actcatcact
     4681 taaataggcg atttcatcag gatgaatttc taagattact ggtgcttcta atctctctgc
     4741 ttcttcgatg attgctttca tcatatcata gctgcaaata ttgaaggctg gtactgcaaa
     4801 ttgattctct ttagccactt tcaataactc tttcattgta tataacatta cgcttccacc
     4861 tcatttgtta gattttcatt tttcgtccac ttgtcttgtt ttttctcttt cactgttttt
     4921 ttcaatagtc caagcataaa ggctgtagtg actgtacctg atgcaattgc caagacatac
     4981 ataggtaaat tgttcataac atttggaatt agcatcaccc aaatccctcc atgaggtgcc
     5041 attgaagtaa ctcctactgc catggaaatc cctgcagcga ctgctgaacc tgtcatcaaa
     5101 ctaggtatca cgcggatcgg gtcagatacc gcaaacggta tagcaccttc tgtaatatac
     5161 gaagcgccta aaatccagca tgattgacct gaagtccgtt cttctttggt aaatttatgt
     5221 ttgaataata aagtggctaa agcaatacct aggggaggtg tcattcctgc agccatacaa
     5281 gcagcaatcg gtgcatttac tccagctgac atcaaaccga tcgagaaagt cgaagtcgat
     5341 ttattgatcg gcccacccat atcagctgcc atcatgatcc cgataagtaa tccgaacaat
     5401 agcccggatg tatttcccaa gctattcagc caatttgtca aagcatccaa cacccatgtg
     5461 atcggttgtt caatcacaaa taccatcaaa agcccaataa ttaagatcga taaaaagggg
     5521 acgacaatca gctgataaat agcagaaaat tcttttttta cgctaatttt atttgccaga
     5581 aaatcacaga tataccctgc tagcacgcca ccaatcattc cgccaagaaa acctgaacca
     5641 cctgtagagg ccagtaatcc ggcaacaatt cctggggcaa aacctgcttt tccagcaatc
     5701 gatgtagcaa tccccgcgcc taacgcagga accatcatgg caaacgctgt atctcctccg
     5761 atccttgaaa atgcagctgc taacacatta tagtctgctg agttgggatc agccgctgta
     5821 atcccaaagg caaaactcag tgcgatgatg attccgcctg cgatcacaaa tggaagcatg
     5881 taattcacac cattcatgaa atgattatag attgtcggct ttttctcatt tgcagctggt
     5941 tctttttcat tagacacaac gaatacgcca atatctctca atgcttcgtc tattactact
     6001 tgcggttctt tgattgctcg gcttgtagag acacgcttta cttttttccc ggcaaatcgg
     6061 tccaattcga tttctttatc tactgcaaga atgattgcat ctgcctcttt gatttcttgt
     6121 tcagataact gattttctgt ttttacacct tgtgtttcga attttgcttg atggtttaga
     6181 tttcttgctg ttacttctaa tttttctgcc gacatataag tatgagcaat tccagtcggg
     6241 caagcagtta caccaacgat tttcatgatt attttcctcc tttttcttca ctatcgatac
     6301 tcggacaaag actctactat tttcattctt gactaatctg tctgatgata tctgctggcg
     6361 tagtggcttc ttttattccc tttctaaaat ctgcattcat caatcgtctg gagagtgtgc
     6421 tgagaatctc tagatgcata tctgctgatt tttcaggaac tacgatggca aaaatatatt
     6481 caattggcat ttcatctatc ccgctatagt ccgttaattt ggctgtatga aaaaaacaca
     6541 gtgctggttt tgtcacatat ttgctttttg catggggaat cccaaatcca tcttctattc
     6601 ctgtggagat ctcctcctct cgtttgaata gtgccttaac gactttttga acatttctta
     6661 ccttatttgt ttttttcaac acatccccta ttcgctcaaa taatgatcct tttgaatcta
     6721 tcgatggatc gaaaacaatc agttcttctg cgatcaattg atcaaacctc ataaaaaacc
     6781 tctcccccac aaaaaatgtt aacgttttct tggaactgag gtcagtatag catcgctagc
     6841 tgtaccgttc aatattatga atgaacagag gaaaaagacc catcatccgc gtgtataagt
     6901 cattaaaatg aacgatctag ctcacaaaca atcgcaagtc tataacaaaa caaaaagcct
     6961 tccttgaaag gaaagcttag cttctaattt taggtaaatg gttttttact tcgaaataac
     7021 ccctcttcat ttcgttaata taaggtgttt ttatcaagtg gataattaaa actatatact
     7081 agtttttcat tgttttgtgc attatgatcc tacgactttt tcagcatcaa acgaagtttt
     7141 aaattggcaa aaaacataaa aaaacagact atccctatca gatagtctgc taaacgttga
     7201 gttatcagcg tttttattat tccgcagcgt ttgcgtcttt gagaccgtat tttttgttga
     7261 aacggtccac acgtccgtct gcttgtgtga atttttgacg tccagtgtag aatggatgtg
     7321 agtcagaagt tacttcgaca cggatgactg ggtatgtatt accgtcttcc cattcaactg
     7381 tttcttgtga agtttttgtt gaacctgaca agaatttgaa accagttgtt gaatccatga
     7441 atactactgg gtggtaatct ggatggatgt tttctttcat gattttttcg ctcctttgcc
     7501 ctgctccatt tgctgaacca gagttgattt gtcgatttac aacattaaca tcttaacatg
     7561 tttcagtcaa ggttgcaatt atcttttcag atttctggtc ggattttttt taccaaagga
     7621 gacatcttga aattctttga aaaattgctg attgtttttt gttttacgca aaaagcggat
     7681 cagttgttcc gtatattcta atgaatctcc agtcatattt tttcgaagtt tccaaatttc
     7741 ttctaattct tcacttggca tcaataattc ttcttttcgg gtacctgatt ttttgatatc
     7801 gatggctggg aagatccgac gttcagataa ttcacgtgaa agttgtaatt ctagatttcc
     7861 agttcctttg aattcttcat agatcacgtc atccatacgg cttcctgtat cgactaaagc
     7921 ggtcgctaaa atcgtcaaac tgccaccttc ttcgatgttt cttgctgccc cgaaaaattt
     7981 cttaggttta tacaatgcgg ctgggtcaat cccgccactc aatgtacgtc cgcttggtgg
     8041 aacgactaag ttataggccc gagcaagtct agtaatacta tccatcaaga taacgacatc
     8101 tcgtttatct tcgactaaac gcattgcgcg ttccaatact aattcagaaa ctcgagtatg
     8161 gttttgcggc tgcagatcaa atgtagaaga taccacgtcg cctttcacgc ttcgttctag
     8221 atccgttact tcttccggtc gttcatcaat cagtagaacg attaattcta cttccggata
     8281 gttttcggtg ataccatttg cgatttcttt taatacagaa gttttccctg cttttggcgg
     8341 tgcgacgatc aatccacgct gaccaaaacc gattggagaa aagatatcga tcattcgggt
     8401 agatagtctt tgcggtgttg tttccagttg aagctgtctt tgcggataaa gaggtgttaa
     8461 ggctggaaaa tgcggacgtt cttttgcttc ttccggatct tttccattaa cgctttccac
     8521 gtgcatcaaa ccataataac gttccgattc tttaggtgga cgggcttttc ctgccacttt
     8581 gtccccattt ctcaaactga atcgtctgat ttgtgaggca gaaatataga tatcttctgc
     8641 acttggtcca taattgattg gccgtaaaaa accgtaacca tcttgaccaa cgatatccaa
     8701 tattccttcc atataataaa atccttgttt ctctgcttgt gcacgaatca cagctagaga
     8761 taattccttt ttattcatct tactgtaata aggaatttta aattctttgg cataactata
     8821 aacttctttt aacgttttgt tctctagttc tgccattgtc aaataatcgc tcattcagct
     8881 cccacttcct ctatcatttc aattgatgcc cctaaggctg ttagtttctc gataatatga
     8941 tcataaccgc gtaagatata atctacattg taaatcgtcg ttgtcccttc tgccaatagt
     9001 ccagcaatca ctaaacaagc acctgcccga agatcagaag ccacgacttc tgtgccgtga
     9061 agtttatttg gaccattcac gatgatcata tttccttcca cggtaatatc agcgcccatt
     9121 cgtgctagtt ccggcacatg gttcacacgt ttcgcataga tcgtatcgat gatttctgaa
     9181 gtgcctgtag tcatcaatag taacgcagta agtggctgct gcagatctgt ggcaaatcct
     9241 ggataaggat aggtcatgat gtttgcgggt tttaattctt tcgaggggta aacttcgatt
     9301 tcatcttcgc tgattttcat atggacacca atttcctgca attttgctat aaagctttcc
     9361 aaatgttcaa agatcacatt tttcactttg ataccatttc ctactgcagc tgctaatgct
     9421 agataggttc ctgcttcgat gcggtcagga atcatgaaat gccgacagcc atgtaatgtt
     9481 tcgactcctt cgatacggat cacatctgta cccgctcctc gaacttttgc ccccatatta
     9541 ttcagtaaag ttgccacatc tatgatttcc ggttctcgtg cagcgttttc aattactgtc
     9601 tgtccttttg cttttacagc agctaacatc acattgatcg ttgctccgac tgaaaccacg
     9661 tccatgaaaa tccgattgcc acgcaatcct ttattttctg tccgtaaata catggcacca
     9721 tgttcatttg tcacttcagc acctagagct tcaaatcctt tgacgtgtag gtcaatcggt
     9781 ctagggccta aataacagcc acctggcagg cctactaccg cttcgccaaa tttaccaagc
     9841 agtgttccca taaaataata agaagcacgc aaactattga tttttccgtg cggcatcgga
     9901 atcgatacga cttctcgagg atcgatttct agtatgttgt
//
LOCUS       DACCQI010000100         9895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCQI010000100 DACCQI010000000
VERSION     DACCQI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.104"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            4..360
                     /locus_tag="IVO76_002193"
     CDS             4..360
                     /locus_tag="IVO76_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430133.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            376..726
                     /locus_tag="IVO76_002194"
     CDS             376..726
                     /locus_tag="IVO76_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP7430134.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            755..1534
                     /locus_tag="IVO76_002195"
     CDS             755..1534
                     /locus_tag="IVO76_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP7430135.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1545..2222
                     /locus_tag="IVO76_002196"
     CDS             1545..2222
                     /locus_tag="IVO76_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP7430136.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2241..3347
                     /locus_tag="IVO76_002197"
     CDS             2241..3347
                     /locus_tag="IVO76_002197"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP7430137.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3337..4428
                     /locus_tag="IVO76_002198"
     CDS             3337..4428
                     /locus_tag="IVO76_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP7430138.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4441..5181
                     /locus_tag="IVO76_002199"
     CDS             4441..5181
                     /locus_tag="IVO76_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP7430139.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5469..7409
                     /locus_tag="IVO76_002200"
     CDS             5469..7409
                     /locus_tag="IVO76_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7430140.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7496..9418
                     /locus_tag="IVO76_002201"
     CDS             7496..9418
                     /locus_tag="IVO76_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7430141.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9698..>9895)
                     /locus_tag="IVO76_002202"
     CDS             complement(9698..>9895)
                     /locus_tag="IVO76_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7430142.1"
                     /translation="LIITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSEEEILTEF
                     RKVREGIKRKVLDLNQRTSIV"
BASE COUNT         3227 a         1641 c         2200 g         2827 t
ORIGIN      
        1 atcatgatta aagtagttat tgcagaccgt atgggtaaag gacaaaatgt ggccaaaggg
       61 gtagaagcag ctggtggaaa agcagttgtc gtaccaggaa tgggggcaga tatgcgtttg
      121 ggcgatgtga tgcaacaaga gcatgcagat atgggtatct ctttttgtgg tagtggtggt
      181 gcaggtgcat taacagcagc aacaaaatat ggctatccag aacgacatgg tatgcgttcg
      241 attgaagaag ggatcacagc tatcaaggat ggcaaaactg ttctaggttt cggctttatg
      301 gatcaagagg aattagggaa acgtctgaca gaagaattta tcaaaaagca tggaaaatag
      361 aaagagggac atgaaatgag cgttcaaaag acaattcagc aaaaagttat cgtctccggt
      421 aaaggggaaa caaagcaaca tgcttttgca tcagcgttag cagaaattca aaacaaaata
      481 atggaacaat caaacgtatt attgcgaatc gaaccagtca atgtggatat tttaaaggcg
      541 gtcgaaaaca aatacacaga acgctttcta tttttcttct tcccacggca acgtattagc
      601 tatgaagtcg aactctctgt tactgtggct gtaacagaaa taaaattggc agacatatcg
      661 ttcagccagc aaatgactga agatccaaat gggattgcca ttccatttgt aactaaaaaa
      721 atctaatttt gaatcataag ggggaaagaa taacgtggat ttcgtaatca ttctagtcaa
      781 atcggtcatt atcgggggat tacttggttt tgcagcatct gtcggggcag ctcgaatgtt
      841 tcatgcacct aatacacaag gtttaggagc ttttagaaca ttaggcgaaa tgaatgcctg
      901 catgggtgat ccagcttctc atttttcatt tggtttaggc tttttcttta atgcatgggc
      961 ttcttcagtt ggtgctggtg cattcacaca agatgtgacg catcgaatca ttcccaattg
     1021 ggcagcggca gcacttctag caaaaaacaa agatgtcacc cagaccatgc atgatccgaa
     1081 aaaaatgggg atcattggag cggttatcgg ggcagtggtg gtagccttct taaacacaac
     1141 agctgcagca attccagagg cgttacaagt gacggcggtt tccgttttgg taccagcagc
     1201 gacgattctg atcaatacag taatgccagt tattttttgg ttggcagctt tagacgcagg
     1261 gaaacgaact ggtttttggg gcactctttt tggtggtatc gcgcaattga ttatgggaaa
     1321 tgcggtacct ggtgttgttc taggaatctt gattggtaaa ggcgtcgatg agagcggttg
     1381 gaatcgcgtc acgaagagca tgatgatcgc tattgtttta ttatttattt tcagtggatt
     1441 tttccgtgga tttgatatgg aaatgatcaa gagcttccag cttcaagtgc ctaattggct
     1501 tgataacttg cacaacatct ttagtgtgaa ataggaggag aatcatggag acaacagaag
     1561 aacaagcaaa agtattagaa gcaaagcggc aaaagagctt ttggtttgca gactggtctt
     1621 ttccgctaat cgtaggaatt atgtccgctg ctgtatttgc aggaacacac atgtatgtga
     1681 tgtatggtgt gggtgcattc aatgaagttt cgatcgtggc gatgttaaaa gccgggctgg
     1741 atggcggatc ctatggcgca gcggcagcgt ttggtgctag tttcttattt gccagaattt
     1801 tagaaggctc tttagttggg atcttggact taggcggttc gatcctaact gggatcggaa
     1861 ttggggtacc agcaattctc ttaagtatga aaatagcggc accaatcgat aattttgcac
     1921 tctctttatt gactggtttg gttctaggtt tgattgttgg tggcatcatt gtcttgattc
     1981 gtaaatttac gatcaatcaa tcaaattcca cttttggtgc tgacgtcatg atgggggcag
     2041 gaaattcatc tggacgtttt ttgggtccat taattatatt aagcgcgtgt gcagcatcta
     2101 ttccaatcgg cattggcgct acattaggag cactaggatt ctatgcttgg aaaaaaccaa
     2161 ttgctggagg tgctatttta ggagcaatga ttttaggagc aatcttcccg gcagtaaatt
     2221 aatagaaaga gggtcattaa atgtttgata ctttgattaa aaatggaagg ttagttgatg
     2281 gatctaaaat agaggtcgct atagaaaaag gcatgattaa agcagttgca gcagaaataa
     2341 atcagccggc gcaagaagta gttgacttgg agggaaagta ttatttatcc gctgggtgga
     2401 tcgatgatca tgtccactgc tatgaaaaga tgaatcttta ttatgactat cctgatcaga
     2461 taggggtcga aaaaggtgtg acgacggtca ttgatgctgg aactacggga gcagaaaaca
     2521 tccgtgaatt ttatcaattg acgaaatcgg tcaagacaaa tgtgtatgcg ctaatgaata
     2581 tctctaaatg gggcattgtc aaacaagatg aacttgctga tctaacaaag attcaagaag
     2641 atctagtaag tactgcgtta gcagaactgc cggaatttat tgtcgggatt aaagcgagaa
     2701 tgagtaagac agttgttggt gaaaatggca ttaagccatt agaattagct aaaaaaatcc
     2761 agacagaaaa caatgatctg ccattgatgg ttcatatcgg ttctgcacct cctgagttag
     2821 aagaagtatt gtcaagaatg gaaagaggcg atgtattgac ccattgtttt aatggaaaac
     2881 caaacggtat tttagatcca aagacagatc aaattaaaaa ctttgtctgg gatgcttaca
     2941 ataatggtgt cgtctttgat attggacatg ggacggatag ctttaatttc catgtggcag
     3001 aaaccgcact aaaagcagga atgaaagcta cctctattag tacggatatc tacatccgca
     3061 atcgtacaaa tggcccagtt tttgatctag caactacttt agaaaaattg agggtagtcg
     3121 gctattcttg ggaagaaatc attgaaaaag ttacagcagt tcctgcacgg aattttcatt
     3181 tgaagaataa gggccagtta gctgtcaatt atgatgcaga tttgacgata tttgagattg
     3241 ttgaaaatga aaaggaattg aaagattcca acggatttac acgtatagct aaagaacaaa
     3301 ttttaccgat taaaacaatt attggaggcg tcatatatga caattagcta tggaaagttt
     3361 gatttaaaag aagtcattaa tgcatcggga aaaatgacga tcctaggggt ttctaaggta
     3421 tctgaaagtg tcctagcagc acaacgattt ggaggcgagc atttttttga gatgagtgat
     3481 cttgccatca aaaccggcgc ttatctttct caattactga aagtggaaga tgcgcaaatc
     3541 gtttcttctg cttcagcagg gattgctcaa tctgttgctg ctttaatcgg acagggcgac
     3601 atgtaccatg tctatcatcc ctatacagaa aaaatcgcta gacgggaaat catcatacct
     3661 aagggacata atgtagatta tgggacaccg gttgaagtaa tggttgctca aggtggagga
     3721 aaagtcatag aagctggcta tgcaaatatg tgtacaccgg aacatgtttc catgatgatt
     3781 acggaaaaaa ctgctgcgct tctatatatt aaaagtcatc atgcagttca gaaaagtatg
     3841 ctgacggtgg cagaaatggt agaagttgcc aaagctcatc gtttgccatt gatcgttgat
     3901 gcagctgcgg aagaagattt attcaaatat agcgaaatgg gcgttgactt agtgatttac
     3961 tctggggcaa aagcaatcga aggtccgagt gccggactgg tcataggaaa aaagaagtat
     4021 attcaatgga ttcggctaca gtctaaaggt attggccgag caatgaaaat cggtaaagat
     4081 aatattttag gctttaccca ggcactagaa gattatttaa aaaatgggag cgaatccggt
     4141 gactccatga agcaacgatt agcgcctttt gttaacgcaa tcaatcaact tcctgacctt
     4201 tcagcgaaaa tagttcaaga tggtgccggc agagatattt atcgagcaag tgtaacgatt
     4261 aatggcgata aaagtgcgaa agaagtcatt caggaattaa aagcacgaaa tccagcagtc
     4321 tacacccgag aatatcaagc aaataacgga atcattgagt tcgacattcg ttccgtcagc
     4381 caaaacgaaa tggaaaaaat tataaaaaga ttgagagaaa tcatgtagga ggcttttaaa
     4441 ttgtcagtta tcccaaatta ttataaaaat cgtgtatgtt taaatgtatt agctcattca
     4501 gttgaaaatg caaaagcatg ctatgaagca gcggaagggc atgtcgtact aggtgtgcta
     4561 tcgaaaaatt atgctagcga tgaagcagca atcgaagata tgaaaaggta ccaagacgca
     4621 accaataatg cgttgtcagt tggattaggc gctggagatc caaaccaaag tcagatggtt
     4681 tcgcgtttat ctggggtttt acaaccacaa catgtcaatc aagtatttac tggcgtgggg
     4741 acttctcgtg ctttattggg ccaaaatgat acagtcatca atggattggt ttcaccaaca
     4801 ggtaagatcg gctatgtcaa tattgctaca ggtccgttaa gttcacaagc gccagcagct
     4861 gaagtgccta ttgaaactgc cattcaaatg ctgcaagaca tgggaggtac ttcaatcaag
     4921 tatttcccga tgaaaggctt agcacatagg gaagaattta aagcagttgc ggaagcttgc
     4981 gcaaaatatg aattctattt agagccaaca ggaggaattg atctttcaaa ctttgaagaa
     5041 attctgaaaa ttgcaatcga tgcaggtgta aaaaaagtga ttcctcatgt ttatagttca
     5101 attatagata aagaaacagg agatacgcgt ataggagacg taaagatact attaagcatg
     5161 gtaaaaaaaa ttattcacta actcattaga aaataagaag cttattccag aatgaaatta
     5221 aatggaataa gctttttata attaattacg gaaattgtta atgtagataa taatttagat
     5281 gtacgtaagc agaagtacag aaatgtaaga ttttccaata ttattttatt caacataata
     5341 aatcgtatat caattttttt agcgaaattt ctatttattc caagaatttc agttattacg
     5401 gaatcatttt gtgcttaata aaaagaaacc gtttttatat actataaatg tagaaaggcg
     5461 gtaggaaaat gaaaaaaatt attgcaatca catcctgtcc agttggtatt gcccatacat
     5521 acatggcagc agaaaactta gagaaagcgg ggaaagcagt tggcgccgag gtaaaagtcg
     5581 aaacacatgg ttccatcggt attgaaaatg aattgactgc aagagatatc gaagaagcag
     5641 ctggtgtaat catcgcagca gatactaaaa tagataaatc acgattcggc ggtaaacctt
     5701 tgataaccgt aggtgtgcaa gaaggaatcc accatgcaga tgagttggtg agagatattt
     5761 tagcaggaaa agcgccagtc tataaaagta cggaggcaat tatttcttcc gaaaataaaa
     5821 gtgaatcaga aaatttaggt aaaaaaatct ataagagttt gatgaatggc gtttcgtaca
     5881 tggtaccttt cgtagttaca ggagggttat tgatcgcgat ttctttaaca ttaggaggaa
     5941 cagctacacc tgaagggatc aagataccag aaggaacgat ttgggcaacg atgaatagta
     6001 tcggaagtat cgctatgggg ttgatggttc caattctatc tgcatttatt gcgcaaagta
     6061 ttgcagatcg tccaggactt gttcctggat ttgttggtgg gatgttagcg gcaaacggag
     6121 cattatatgg tagtgatgcg aatgcgggat tcttaggcgg gatcatcact ggatttttag
     6181 caggattcat tgctttagga atcaagaaag tgcgagtacc gaaagcttta caatcgatca
     6241 tgccaattat tgttatccca attttaggat cattggcagt tggttttgtg tttatctatg
     6301 ttattggaga accagtagct cttctattta gttcattgac tcacttttta gcagggatgc
     6361 aaggaggaag tgaaattgtt ctagctatga tcttaggagc gatgattgct tttgatatgg
     6421 gggggccgtt caataaagta gcctttttgt tcggatctgg cttgattgca gaaggcaatt
     6481 attccattat ggggccaatt gctgtagcta tctgtatccc tccattagca ttggggctgg
     6541 catcattgat tttaaaaacc aaatttacga aaacagaacg agaagctggt aaggcgtcct
     6601 tagcaatggg actgtttggg attacagaag gcgctattcc gtttgcttca acagatccac
     6661 tacgggtgat tccaagcatt gtaatcgggg caatgacagg aagtgtgatc gcgatgcttt
     6721 caggagtgac agatcatgta gctcatggcg gtccgatcgt agcggtttta ggagcagtgg
     6781 atcacgtagc catgtttttt gtagcagtga ttgctggtat tgctgtaacc gtgctaatgt
     6841 taagaatcct taaaccgaca ttacaacaaa ctgcggttgc tgcagcaaat ggtgtgacag
     6901 tagcagatct atcagagact gaaacaagtg atgtttatac gaaaactgaa acttttgatt
     6961 ctatcacgga tattatgtca gatgaagcaa tcgttttagg tgaaacagca acaacaaaag
     7021 acggagtaat cgaagattta gtccaacgtt tagaaaaaac agcggcgatc acagataaaa
     7081 ccagatttaa agaagtcatc tatgatagag aacatgaaag tacaacggga atcggtatgg
     7141 ggattgctat tccacatgga aaatcagctt atgttcttaa accaacagtg gcatttgcca
     7201 gaagtcaatc tggggtagat tggcattctt tagatggaaa acctgcacat atgatcttta
     7261 tgattgctgt acctgaaaac agccaaggtg atatgcactt gaaaatactg caacgtctat
     7321 cacgtaaatt gatggatgat gacttccgtc aagcactgat ggaagcgcca gataaaacaa
     7381 cagttcacca attattaagc gaaatgtaac ataagatgaa aaatcgaccg tgatgagaca
     7441 taatagatgt gttatcatga tcgatttttt atttagaagg gagtttctgc acattatgaa
     7501 tcagcgtcag caatctttga tctatcaatt aagtgagaac aataattata taacaacaaa
     7561 aaagctagca gagcatttcc atgtgtcaga aagaacgatt catagcgatt tgaataccat
     7621 tgaaacttgg ttgagagaca aacatctgcc tttaatgttt gaaagaaaaa aaggtacagg
     7681 tatcttgttg aatggtctgc ctcacgaaag ggaacaactg aaacgagcgc ttaatgaaga
     7741 gactcaaaca gaaatggata aaaaacagct tcgtcagtta ttgattttcc atctgctagt
     7801 agcaaaagac ggatttagtc ttgaagaact ttcagaaaaa ctatatgttg gaaaacgtac
     7861 gattcgtaaa gaattgacag agttgcaaag cttttttgag tatcaccatt tacagctagt
     7921 atccaaaaca aaactaggaa cttttcttaa aggcgatgaa caagaaaaaa gacagttgct
     7981 tgttaagaca ttacgaaata tgcagaaaga agatccacaa agtccgtcgt tgaaagaatt
     8041 cttcgctaaa gatacattaa aagttatcca acatacatta caagatgttt tttatgaaaa
     8101 ccatttagaa cagccgaatg gactggctag tgtggagatt cacatctact ttatgctcga
     8161 gcgaatgaaa cagtaccaga aagtgaaatt aagtaaagac gaaaacgaag tcgtggaaca
     8221 cacccaagca caaaagttaa gtagtcaaat attagcaaaa ctagccacca tctatccaat
     8281 tgaattttct cctgatgaaa tcaattatct ggctttgcgt atcgccaatc tttttacaaa
     8341 ttcacaagca gccacacgtt ttcaaaagga atctagcgca cttgctgatc atttgatcga
     8401 acaagtggaa cagtttctag gctattcatt aaaagaagat cagctactaa aacaaaattt
     8461 aaggtctcat ttatcttcaa cttattttcg actccattat ggtttgcaga tttctaatcc
     8521 attgacgaaa aatgtgttta gtacgtacac acaacttttc ttagttttac agttaatttt
     8581 agaagattat tttgaaaagg aaaattttta tgttccacaa gaggagacag cttatttaac
     8641 gatccatttc caagcagcga tcgagcgaca caaacatcag aaaagtagaa gatatcaaac
     8701 ggttttagtt agtgaatatt caaaagctat ggctactttt ctcgaagcac ggctgaacag
     8761 agaacttcca gaattaacga ttatggatct agttgaatat aagccagaat taaagagaca
     8821 agattttccc tctgttgatt ttatcttggc tacgttacca tttaaacata taagcattcc
     8881 agtaatcgag atctcaccaa tgatcacaga aactgatcta gcctatttga caaaatatat
     8941 gctggagcat gtgcctatca agaagaaaaa aacatttgat ctagcaagct ttactcatcc
     9001 atttctgatc tttcctcaac ttgaacggac agacccagcc gatattttaa actttatggg
     9061 aaacgtctta gtggaacatc attatgtgga gccggaattc atcgattctg tcttggaaag
     9121 agacagtcac tccagtacta gagtagcacc atttgtaaca atccctcacg gtaatccgct
     9181 gtatgtgaag cattcgatga tttctattgc tacaatgaaa gaaccagtcc tatggcacgg
     9241 ggaacagata cgtatcgtac tgctattagc aataaagaaa aatgatttga aacatgcaga
     9301 gttcaaaaaa atattttctg ttattcacta tctagaaaag cagccagaac aaatggctca
     9361 gttgatgcaa tccaatcatc cgctagaaat cttaacgttg ttatctagtt atgaataagt
     9421 agcgattaaa aaactgcctt tatatttata ataattctat ctatttattt ggaagtagaa
     9481 aactaaatac aaaaaaagag gatgtggcca aagttgtggc acatcctttt attttgagtt
     9541 tcttataatt ttttatagtt gcttacgatg tcttgactac tggtaaacgg tgttcaagag
     9601 acaatttgtc ggcaataagg cacaattttg taaaatatgg aaagctattt ttttaacatt
     9661 gtttcttagt gcttgaaaaa tcattttgaa tacttcttta tacgatcgat gtacgttgat
     9721 ttaagtctaa aacttttctt ttgattcctt ctctgacttt tctgaattcc gttaatattt
     9781 cttcttcgct cccgacagct tgtgcaggat cttttaggtc ccagtgaagg tgttcgtgat
     9841 tgtttggaac ggcagggcac ttgtctttgg catcaccaca aagtgtaatg atcaa
//
LOCUS       DACCQI010000101         9799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCQI010000101 DACCQI010000000
VERSION     DACCQI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.105"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(24..2144)
                     /locus_tag="IVO76_002203"
     CDS             complement(24..2144)
                     /locus_tag="IVO76_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7430143.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2374..3552
                     /locus_tag="IVO76_002204"
     CDS             2374..3552
                     /locus_tag="IVO76_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7430144.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3568..4326
                     /gene="aroD"
                     /locus_tag="IVO76_002205"
     CDS             3568..4326
                     /gene="aroD"
                     /locus_tag="IVO76_002205"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7430145.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4349..4834
                     /gene="ybaK"
                     /locus_tag="IVO76_002206"
     CDS             4349..4834
                     /gene="ybaK"
                     /locus_tag="IVO76_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7430146.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4905..6158)
                     /locus_tag="IVO76_002207"
     CDS             complement(4905..6158)
                     /locus_tag="IVO76_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7430147.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6275..7624
                     /gene="gdhA"
                     /locus_tag="IVO76_002208"
     CDS             6275..7624
                     /gene="gdhA"
                     /locus_tag="IVO76_002208"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7430148.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7736..9082
                     /locus_tag="IVO76_002209"
     CDS             7736..9082
                     /locus_tag="IVO76_002209"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7430149.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9390..9776)
                     /locus_tag="IVO76_002210"
     CDS             complement(9390..9776)
                     /locus_tag="IVO76_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7430150.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3125 a         1781 c         2008 g         2885 t
ORIGIN      
        1 ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc gtgaagttct
       61 tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc actaaagaca
      121 ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa gccattcttg
      181 tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa ccgaagaaga
      241 tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc tttcagtgaa
      301 tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt ttcgttacta
      361 tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc acggaaagaa
      421 acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata attttttgta
      481 tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc gtaagtgtga
      541 ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg ctgcatttgt
      601 gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc tagattttta
      661 tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga attatcatac
      721 aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa ataattggct
      781 gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa tcctgcttcg
      841 tcattggtga attttgaata aggataatgg ttagatacag caatgaattt tgaataaaat
      901 ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt atcatgcaac
      961 ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa gaaataatca
     1021 tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt cccatggaat
     1081 accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg gaaagtattt
     1141 tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga attttccatc
     1201 agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt gtcaaaactg
     1261 aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg ctggagacca
     1321 ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa atggatataa
     1381 atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc ataatgttct
     1441 tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg cacttggttt
     1501 gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag atatttgacc
     1561 aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc cgccataaaa
     1621 agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat tcttgctcgg
     1681 atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa gataataaaa
     1741 tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc tcccagacca
     1801 ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt gaattctcga
     1861 taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac catcatcgtg
     1921 atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa caatgtagtt
     1981 gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc gagatggaaa
     2041 tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa agaaaaaaat
     2101 ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat cttcctttct
     2161 gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt attctcattt
     2221 attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct gtttcattaa
     2281 ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt tttttgttat
     2341 acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa aaaaagagca
     2401 agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc tcatccccaa
     2461 aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat cgcaaaagga
     2521 tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa catcccattt
     2581 gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac tttctttttt
     2641 tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg gatcggcggt
     2701 ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga aacgttatat
     2761 gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat cataggaggc
     2821 tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat ttacggaaaa
     2881 gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt ttatttaaat
     2941 gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc attagcagaa
     3001 ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc cttttctgtt
     3061 gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa acgcagttta
     3121 aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca aaaaatcatt
     3181 gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt atatgacatg
     3241 attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg cgtagcaaaa
     3301 gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg gatacttatt
     3361 gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat tgaagaggaa
     3421 ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc aaaagatttc
     3481 cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta tcaaattaaa
     3541 aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa tataggaagc
     3601 ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt attacgtgaa
     3661 gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga tcattatcgt
     3721 gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga gatgcttgat
     3781 ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca agacatatcg
     3841 atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt agatatggtc
     3901 gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga aatcaagtct
     3961 ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc agaccctgtc
     4021 ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa actggcagta
     4081 actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg ggcaaatgct
     4141 tttgtctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat ttctcgtctg
     4201 gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga ttctacttct
     4261 gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt aaatgaaccg
     4321 ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa aaacgaatgc
     4381 aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg agtgggacga
     4441 aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat ctcgtatatt
     4501 taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga taccgggaga
     4561 agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag tagaaatgct
     4621 ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct cgcctatagg
     4681 tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg aacaaatcat
     4741 tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata tctgtgcatt
     4801 aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag aaagaaggag
     4861 gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc tctttgcccg
     4921 gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa gccgccagta
     4981 taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat tttactctcc
     5041 aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa tgatttctca
     5101 ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac aaaatcttga
     5161 atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg ctcaagaaag
     5221 aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt ctttttggtt
     5281 gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt agggaaacaa
     5341 tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac aaaacttgaa
     5401 caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc catcgtatgt
     5461 tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg taaagaaagc
     5521 ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga ctctgtcgca
     5581 taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc tccagtagaa
     5641 ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc atggaactct
     5701 tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt ctctactcga
     5761 gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc ttcgataatc
     5821 gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt taaatgtacg
     5881 ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa cgtactatat
     5941 aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg attgttcgta
     6001 acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt gtttttttcg
     6061 atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat cccgcttgtt
     6121 ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca aggaaagtgt
     6181 agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga aaatgagcta
     6241 aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag aatatgttca
     6301 agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt tacaagcaat
     6361 cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat atatcgaaaa
     6421 gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag tgccttggca
     6481 agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca actcagctat
     6541 cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta tcttgaaatt
     6601 tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg gtggaggaaa
     6661 aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc gtttttgtca
     6721 aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc ctgcgggaga
     6781 tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac gattgaaaaa
     6841 atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta agatacgtac
     6901 ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg aagaaaaaga
     6961 ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca tttatgcaat
     7021 cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta gcgggtacat
     7081 ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa tcaatagaca
     7141 acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg gagaatctgt
     7201 ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa atgaaataga
     7261 cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag aaggcgctaa
     7321 tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt ggtattgtcc
     7381 aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga gtcagaacgt
     7441 ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa tcatggaaac
     7501 gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa atctgcttct
     7561 tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag aaggtttagt
     7621 ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc ttgaaaaata
     7681 ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag aacaaatggc
     7741 acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag agttagggta
     7801 catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg gcgcaggaag
     7861 cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat ttgcacgtat
     7921 caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta tcggtatcgg
     7981 tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat tcttcaattt
     8041 attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt caatcagctc
     8101 aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag taaacgttat
     8161 ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca aagagcttct
     8221 tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa cagataaagc
     8281 aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg tgattcctga
     8341 tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa tcgctgttag
     8401 tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag catactcaag
     8461 cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca ttctttaccg
     8521 taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat acttctcaga
     8581 atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca tctatccttc
     8641 aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag aaggacgtcg
     8701 caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca caattcctga
     8761 acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg acttcgtaaa
     8821 tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc ctaacttgct
     8881 agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact tctttgaaat
     8941 tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac caggtgtaga
     9001 agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag atcttgctaa
     9061 agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata atcagtgata
     9121 gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa catactgcat
     9181 cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg tttgtataga
     9241 aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag tgaggctgac
     9301 ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa gcactcactt
     9361 cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc ttttacagat
     9421 gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc gcttgctttc
     9481 aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc agaagtaatg
     9541 acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac gttgtattta
     9601 aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc aggcattgga
     9661 gctttgacta ccgcataata atctttacct acataagtag actgatagta gtcccaacct
     9721 ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt tttcatgaaa
     9781 gatctccttt tttattttt
//
LOCUS       DACCQI010000102         9714 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCQI010000102 DACCQI010000000
VERSION     DACCQI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.106"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(151..774)
                     /locus_tag="IVO76_002211"
     CDS             complement(151..774)
                     /locus_tag="IVO76_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430151.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(933..2330)
                     /locus_tag="IVO76_002212"
     CDS             complement(933..2330)
                     /locus_tag="IVO76_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7430152.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2362..3333)
                     /locus_tag="IVO76_002213"
     CDS             complement(2362..3333)
                     /locus_tag="IVO76_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7430153.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3707..4132
                     /locus_tag="IVO76_002214"
     CDS             3707..4132
                     /locus_tag="IVO76_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430154.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4620..5321)
                     /locus_tag="IVO76_002215"
     CDS             complement(4620..5321)
                     /locus_tag="IVO76_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7430155.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5337..5777)
                     /locus_tag="IVO76_002216"
     CDS             complement(5337..5777)
                     /locus_tag="IVO76_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7430156.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5777..6352)
                     /locus_tag="IVO76_002217"
     CDS             complement(5777..6352)
                     /locus_tag="IVO76_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7430157.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7200..7679)
                     /locus_tag="IVO76_002218"
                     /pseudo
     CDS             complement(7200..7679)
                     /locus_tag="IVO76_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7838..8539)
                     /locus_tag="IVO76_002219"
     CDS             complement(7838..8539)
                     /locus_tag="IVO76_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7430158.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8647..>9714)
                     /locus_tag="IVO76_002220"
     CDS             complement(8647..>9714)
                     /locus_tag="IVO76_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7430159.1"
                     /translation="YDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLER
                     YSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGH
                     ELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTH
                     KKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKN
                     PFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRID
                     YLRRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLN
                     RSKKKSFDWYKEVIASNGVNL"
BASE COUNT         2752 a         2035 c         1581 g         3346 t
ORIGIN      
        1 ttaaacggca ggctcaagac ctcttgatac acggtgttca atcagctgac cttcggtatt
       61 agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg
      121 tcaaaacgct gatttcacaa cctctgtttt tcattgtcca taggattgat attgtccttg
      181 tgatgactca ttcgtaataa ttgaggcttg attttcttca cttcccgtta tcgtttgtcc
      241 actgccagaa tctgaaggac ttgttcctgt cgactcgttt ttttgagaag agacagttgt
      301 tttcttgact tgttcatttg tctttttcca ttccgctaaa cgatcggcga ccactttttt
      361 gtctactttt tcagaataag cggtgatcac ttttccattt ttcaaacggg ctactttgtc
      421 gccatcatga gtcaaaagca aaacggtcaa atgcttgtct ttaaattgat attgtctgag
      481 agaaatcgca ctaagtacgt catcgatatt aataaattga ttttcttctt ttccttttgg
      541 caaataaatg tccaattcgc ctttttcgta attggcagtt attttatact ccgcccacca
      601 ataagttgag aattcttttt tgaaattatc tactgattct ttgcttgcta cgattttttc
      661 tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag
      721 cagtaagaaa aaaccgaata accaaaagag aagaaacggt ctttttttcc tcatgctttc
      781 ctccactcct atttaatttt gactattcta ttatatacaa aaaatgaaat agaaagacaa
      841 aaaagttgaa aaagatcgta aataattgaa cttctttttc acaaaatggt aagaatggag
      901 aatttcacgc atttgctctc aactattctt tcttaagcct caaatccgtt tttatctgat
      961 acgtctttga accaatgacc cgattttttg attgttcttt ttccatcttg tgctaagtct
     1021 actgaaataa agccatatcg attcttatat gcattggtcc acgaccagtt atccatacat
     1081 gtccacatat gatagcctaa acaattggca ccttcttgaa tggcttgatg gacatatttc
     1141 agatgagatt ggacaaattc gatccgataa tcatcgtgga tcatgccatc ttctttgata
     1201 aatcgtgctt cgttttctac acccataccg ttttcagaaa taaagcagtc gatatttcca
     1261 taatgatctc gtacattgat caaaatatca tagattcctt gttcatagat ctcccatccg
     1321 cgataaggat tcattttacg accaggcatt tcataattgt cgaaataatc ctcaggcatt
     1381 ggaccgtttt tagtatcgat tggtgtttct tttgctttta cccggcgtgg ctggtaataa
     1441 ttgataccta aaagatctac tgtattttct ttgataaccg tcaaatcctc tgcatggatc
     1501 acaggcatga gatcaagttc ttttactatc tcaatcaatt cacttggaaa ctcgcctttt
     1561 acagctggat caaggaatga acgattgaag aacgcatctg ctatttgcgc tgctttaaca
     1621 tcttcagggt ttgattcgtc tcttggatag cttggtgtca gattcaagat gataccaata
     1681 acaccacctt gctccatttc atggtaagat ttgatggcta acgctgaagc caacgcttca
     1741 tgatagccaa cttggatcgc ttttttcata tctgactctt ctggataatg ccatcgatat
     1801 aaataacctg cttcgactgg aacgattggt tcattatgag taaaccattt ttttactttc
     1861 gaaccaaaca attcaaaaca acttttggca tatttttcat aggcttcaac cgtttctcgg
     1921 ttcaaccatc cgcctttttc ttgcagatac atcggcatat cgaaatgata cagattcaca
     1981 aagggttcga taccgttttc aatcaactca tcaaaataac gatgatagaa acgaacggct
     2041 tcttcgttga ttgttccaga ttcatcaggt atcaaacggc tccattggat agatgttcga
     2101 aatgaattat gcccagtctg tttcatcaac tgtacatcct cttgatactt cgtataaact
     2161 tgcgaggttt tttcaggtcc tacttgatca aagaactttt gcggttcttt tttgtaccaa
     2221 taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc ttccttctgt ttgtgggcca
     2281 cttgctgcag aaccccacca aaaatttgct ggaaattgat attttttcat caaagcacct
     2341 ctttttcttt tttagttcgt tttagttgat tccatgtatg atattttcca tcacaatcac
     2401 tttttcattt tcttcctctt cttttttgat tcgttttaac agctgttctg ctgctcgttc
     2461 cccgatttta tctgttggtg gcgtgatcgt gattttcgga aggatactca gatcgttttt
     2521 atctaaataa tcatagtgac tgaacgcaat atccttcccg attctgacac cttcttgatt
     2581 gaaacattcg atggcaccaa tcgacatatt gtaattgctg acaaaaatag cagttgcccc
     2641 tttttctaat aatgcttttg ccccgtaata gccgcttttt ctagaatagt ctcctgtaaa
     2701 gacctctaat tggatcgatg gatcattttg gaccgcttct ttggctcctg ctaaacgttc
     2761 tcttgctacg tagtctgttt gagtggcagc aataaatccg atttttttat gtccttgttc
     2821 accatgatga gcgatcactt ttgctacact ttctcgatca ttcactaata ttgtatcaat
     2881 acctgctggt ccttctggac aattcaatgc gataactgga atactcattt ttgtgagcat
     2941 ttcaaatcca gaccactctt cttcggataa gaagacaatc agcccatcga tcacatgctt
     3001 catcaggtat tcaatttttt gttcaatttt ttctggctga tcggcaaacc cgcttaataa
     3061 taagctatac ccattttctt cagctactct ttcgatccct gagacaactt ccgacccaaa
     3121 acggctggaa atcccgttta tcaaaagtcc aatagagaag cttcgattat tcttcaaccc
     3181 tttagcaatg atgttttctt tatagtccaa cgctgaaatc gcttctgcta tttttttcat
     3241 gttcttttcc ttcagttgct gaccattcaa gtaacgggaa actgttccca ctgaaacacc
     3301 agccatctta gccacatcac ggatcgatgc catacattct cactcctgcc agttacattt
     3361 gatttctctt tcttgttgtt cgattatgat gaccgcattc tttgcatagt gttgcttgtc
     3421 atcaatatcc atataactaa agtcttcagt agagcaactt cctctcacta ttaattatac
     3481 ttaaacaagt atttgaaccg gttcattttt aatatagcat ttttttaaaa gattgcaaca
     3541 cttttttaaa acgctttctg aaagtggcgg attttttgcc tttttaaaat tttattctaa
     3601 aatttactct atttgtgggt gttttgtgcg tttttctctt ccatccgttt gaagttttac
     3661 attccaatgg tataatttct ttatcctatc aaatggggga agtattttga agaaattaat
     3721 cgtactattt attttgtctg cgggactttt gacattgact ggctgtgatt tgtttggcgg
     3781 ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc ttaaaagcgg aaaaagcaga
     3841 taccaccaac agtgtatata cgctaaaagt acgtaaagat atggatttat ccgcgggaca
     3901 gttgacattt acagataaaa aaatggaatg gacacgcacg tatcagacac aaggaactga
     3961 ttcttctgaa tcatctgaag aaaaaacagt gctgacagat atcaagatca ctaccaaaga
     4021 tgacgaatat attatttcag gaaaagaaaa taaaaaagaa acaacagtca tatttacaaa
     4081 aatcggtaat taccgtttga aggatgagga tggcaacatc tacagtctat agtgccgtct
     4141 gttggatctt cttttgtttc tccccttctt ttctgctata gtaaagaagc aagatgattg
     4201 taaaggagac gaaatggatg atcgatagtc ctgattattg tacatgtaat aacaacaaaa
     4261 atatccatac agaatttaat gaccaaggaa gcttgagcat atgcgacaac tgtcacaaac
     4321 tagtcaaaga ttcatttaaa acatttgaat cttatcctgt tcaaaattat gtaaaaagta
     4381 gaagaagata aacaaatcca agcaaaactc actatggctg atttttatga acttcaaact
     4441 cagcttcttc ctttgatcaa gcttcatcag taaaacaaac agtggatcat ttcagtttac
     4501 aaatcaaaga gtaaagattt atttatttca aatatttgaa tataaaataa ccaaacgata
     4561 gaaaggctta ttctacatca cgtagtaatt tttctttccc gtcgtttggt tttttatttt
     4621 tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa aatattttcc agcaggcttt
     4681 ccttccaatt cagctaattt ttcttggatc tgacggatca ccttttttgc atcttctgga
     4741 ttttctacaa aatcgaattt atccccatcc acttgtattt taggacatac atcaaaatct
     4801 tcataccatt gattatagcg ggtgttcaac gtttcataat aggtatataa cgtctcatcg
     4861 ctttctagct gttcatagtc acgcccacgt tttttgatac gttctagcat cgtgtccaaa
     4921 gaaactttga tatggaccat caaatcaggc cgtttttttg gagcaacatc atctaactcg
     4981 tttagcatcg tatctaataa gctatcgtac tgctgtactt cgatatccgt tacccgacct
     5041 aaatcagcgt ttagatggaa caacaggcta tcttcataga tggatcggtc tagcacattg
     5101 tcatcgttca ctaaagcatt tttcattgct aaaaatcgct tgttcaaaaa gaagatttgc
     5161 aataaaaaag cgtactgctc aggatttgaa taaaagagcg gcaatacttc gttatcttcg
     5221 acattttcat aaaatggttt agaatcaatc tcttttgcaa gcatatccgt caaactgctc
     5281 ttacctgcac caatcgttcc tgaaatcaaa atcactgaca tacaaccctt cctcttctat
     5341 aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa aatcatattc acgtacagct
     5401 tggtctgatt ttaaatacgt atggagactt tgtgtaatca tcaagttcgt aggtaccgtc
     5461 tcttcttgta caatgatcat cggttttttc aacatagttg ccacacctaa ttcgtaagcc
     5521 gttcctggat cgattgtttg atgctcaaag tcaaggatag caacaacaaa ttctgcattt
     5581 gtcagttctt ccatatcttt ttcgtatact tcctgcgccc atccagcgct gaatagttca
     5641 taattgcttt cttggtgatg acgtggagag tagaaacttg tgactgtttt gttttcctcc
     5701 aacgcttttt cgatacgact tactcgatcg atttgctcct ctgaaaaaaa tggtcctgct
     5761 aaatatattt gactcattat tttgcctcct tcaataataa ctgattttta atagatactg
     5821 ctcgtaattc attgaccagt tccaatggat tgttggaaaa gattacttga tccaaccatt
     5881 ttttatccat aaaatcctct tttgcaaaag tttctaataa ttgttgcaag ccgctgtaat
     5941 aaccgccaat attcaatatt ccgattggct tgtcgggcgt cagaccgatt gctatccaag
     6001 aaagaagttg tgagaattcc tccaatgtac ctagacctcc agggaaaacg aggacagcat
     6061 ctcccttttc aaataataat acctttcttt catcgatcgt ttctgtagga ataaatgtag
     6121 taacatcttt tccaggcaat tcagtttcaa aaagcccatt cggatagata cctgtgactt
     6181 ttccgttatt ttccagtact gttttactaa tcactcccat aatcccggag tatgaaccac
     6241 cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc taacgtttgc gcaataaatt
     6301 tgtaactttc tttattacca aaacgggaac cgcaaaaaac agtaagtttc ataaaaaaac
     6361 actccctaac tcaaaagtct atcttcattc tacacgtgtt tttttaaaat aaaagactag
     6421 attttgtagt tttttagtgg tatataggat agacgccact atatatagta tttagaaaaa
     6481 aatcattttt cagccatttt atagataaaa caaaaataaa taacgtgttt tcatcgatat
     6541 gaaatgactt acttatagaa gatactggga tttcgtccta tatgccaata acgtgtatta
     6601 ttttaaacaa caaatacttg tttcttttat taacgaaaaa atcattttgt tctcgtgtta
     6661 tgtttcactg ataaatgatg tttctctaaa taaaaaagcc gacgaactaa acgatcaaaa
     6721 tttgatcatt tttgttcatc agcttctcct aaaaatcttt ttgttaacga taaatagctg
     6781 gatttgtaaa aatatcactt gaatcgtcac tgctggaaga aaattcagtg ataaccgcac
     6841 ctgttgcgcc tgcctggaac tagtgtttga tattgggctc gaacgtgtac tgatctctag
     6901 catgaagaat aatttctttt tttactgtat aataactttc tttccttttt ggaatagtac
     6961 aggaaagttt ttttgtttcc tttccttcga cataaggtat accagaccct ttcgacaacg
     7021 aaaggttctc atttttcctt catcgtcatt tcaaggaggg tgccgatgct ctggacagtt
     7081 tcgatcgctc ctaaaccaaa atctgtatat tcgattcttt ctaactctga ttctttcata
     7141 ccattcctgt ctcaaggtac atctattgta cttttttctt gtaatccatt ctgcctctcc
     7201 taactaattg tcaaatcatc ccaagatatg tcttcttcct ctttttcttc ttggatgatt
     7261 tctgcattcg gatctactgg ttttttcaat aataaaatgg ttattcctgt gacaaaagaa
     7321 ccagctaaca atgcaccaat gaatgcccat ggtctggtca tcgtagcgac tgcaaacatt
     7381 cccgatgccg gaatacggct atctgcgccc caaaacatgc ttaatccgcc acctactgtc
     7441 ccaccgactc ccgttgctat gacattgcgt gccaagtcgt tcaatacgat agggaaacat
     7501 ccctctgtga tctgacagat ccccatagga aaagcggtct tcaatgtatc gatttcttga
     7561 cgattgagta tatttttacg aatgattttc cctaagaaat aagcgatcgt caagccgaat
     7621 ggggttacca tcgaagctaa aatcaataca gtgattggtt catagattcc ttctgaaaat
     7681 cgtgccacac ccccttatcc agaataaatg gtaatccaga aacagccgtg tatctgcggt
     7741 aataagccta accaaacgaa aaatatgaga agctattttt gtttggctgt tcttattact
     7801 tgatgcaggg cagctttttc acaacctctt tttttattta tgtttcataa tcaatatggt
     7861 aaggatgcag ctccgtatgg tagaccgcct ccgaatattc aatcatttcc ccaatttgcg
     7921 tagacgattt ccgtatccgt ttgatgccga tctgtgcgtc tgttttcaga attccttgtt
     7981 cttcttctgt taaaaatact gccgagaaat catcagaaaa tgagtcaata gtatatcctt
     8041 ttttcgtcag caaacgatac aaagattctt tgccaaattc ttctctgctg aattgcatca
     8101 tttcttgtgg aagataatga acaaaataaa tataaggctg attgttcaaa taatataagc
     8161 gcttaaaaca aacagcttgc tcaccgaaaa aggaaaacaa aggatcttcc ttctttatat
     8221 ggaccaattg caaatccagc gtttctttcc ggacttcata tcgggagttc tccaagattt
     8281 gcgtaaagct tgccgctttg gataatcgat tgtaaggacg attgcttaga accgtcgtcc
     8341 cacgaccact tttctttgcg acatattcat ctgatgccag cagttcgatt gcctttcgga
     8401 cagtgatttt gctgacttga aacagttctt ctaattcact ttctgtcggc aacatcgaac
     8461 cgactggata ttttcctgag aaaatatctt ctttaatttt atctgcgact tccaagtata
     8521 gtacacgtgt ccttttcata acgtcaccct ttgctttact attactaaaa gaatagcaca
     8581 taaaaaataa aaaaagaaca caaatcacat gtgagaagtg tgttcttttt cattcagctg
     8641 ctttaattac aagtttacgc catttgaggc aattacttct ttataccaat caaaagattt
     8701 tttctttgat cgattcagtg tcccatttcc ttcatcgtct agatccacat agataaagcc
     8761 gtaacgttta ctcatttctc ctgttgatgc actgacaaga tcgatacatc cccaaggcgt
     8821 atagcccatc aaatctacac cgtctgctac tgcattagcc atcgcttcga tatgacgacg
     8881 taaataatcg atccgataat catcaataat cgtgccatcc tcttccatat gatcaatggc
     8941 tccaagaccg ttttcaacaa tgaataatgg tttctggtaa cgattgtata attcattcaa
     9001 tgcgattctc agcccttctg gatcgatctg ccagccccat tcacttgcct caagaaacgg
     9061 attttttact cctcccagca agttcccttc tgaagttgct gcttgtggat cagtctcatt
     9121 gattgccgta gacatataat aactaaatcc gatgtagtcc actggatatt cttttagcaa
     9181 ctggaagtct ttttccgttt gctctaattt ttccggatcg actccatatt ttttatgggt
     9241 acgcgctgtg tatgcagggt attctccacg tacttggaca tccgtgcaga aaaaattgaa
     9301 ttcttggttt tggatcattg ttgctgcctg attgactgga ttggcatcga tgctgtaagt
     9361 tgttgcatag ataatcatgc agccgatttg gatatccgaa cgcaattcgt ggccgatttt
     9421 tacggctttg ctacttgcaa cgaattgatt atgccatgct tggaaaatat tttggtactc
     9481 tcctgctcca ttgcttttta ctaatccttg acttaaagct ggaaagtgaa aggccgaatt
     9541 gatttcattg aaagtcatcc aatatttgac tttactgctg tatctctcta atactgtctg
     9601 agcaaatcgt tcatagaagt cgatcagttt tcgatttttc cagccaccgt attcttttgc
     9661 caaatgaaga ggcatttcgt aatgagaaat cgtgatcaca ggttcaatat cata
//
LOCUS       DACCQI010000103         9689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCQI010000103 DACCQI010000000
VERSION     DACCQI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.107"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            142..1533
                     /locus_tag="IVO76_002221"
     CDS             142..1533
                     /locus_tag="IVO76_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7430160.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(1606..2253)
                     /locus_tag="IVO76_002222"
     CDS             complement(1606..2253)
                     /locus_tag="IVO76_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7430161.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(2261..2947)
                     /locus_tag="IVO76_002223"
     CDS             complement(2261..2947)
                     /locus_tag="IVO76_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7430162.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2953..4026)
                     /locus_tag="IVO76_002224"
     CDS             complement(2953..4026)
                     /locus_tag="IVO76_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7430163.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            4248..4922
                     /locus_tag="IVO76_002225"
     CDS             4248..4922
                     /locus_tag="IVO76_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7430164.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4927..6267
                     /locus_tag="IVO76_002226"
     CDS             4927..6267
                     /locus_tag="IVO76_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7430165.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(6416..7402)
                     /locus_tag="IVO76_002227"
     CDS             complement(6416..7402)
                     /locus_tag="IVO76_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7430166.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            8126..9157
                     /locus_tag="IVO76_002228"
     CDS             8126..9157
                     /locus_tag="IVO76_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7430167.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            9266..>9689
                     /locus_tag="IVO76_002229"
     CDS             9266..>9689
                     /locus_tag="IVO76_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7430168.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
BASE COUNT         3112 a         1753 c         1667 g         3157 t
ORIGIN      
        1 ctaaagggaa tcacaccaat tttcatacca attggtttca tttatgaagt tgattccttc
       61 gattaatgag cgtttcattg aaagaaaagg gttgagggag aaaaacatat gatcaggttg
      121 ttggaaggaa actatagaaa gatggaaaaa atcattcgaa ttctgtccat ttctgacaag
      181 tggatgacta aaaaagaact tgccaaacga attggtagtt cagaatctac atttgtaagg
      241 tatatcgaag agatcaaaat ccgctgggga acggatgtaa cgattcaatc ttcacaaaaa
      301 ttggggtatc gtcttgaaca tttgaatgta ccagcttatt taaatgttct gatggaaatg
      361 tcgcaaagtt ctacaaacag tcgtttatta aatgagatta ttgtcaatcc gggtaaaacg
      421 attgagtatt attgtgaaaa aatttccatc agccggtctt cttttacacg taagttgaaa
      481 caatgcaatc aaattttaga aagctattta ttaaaagtga tcgttgacca aggttttcag
      541 ttaactagcc ttaaaagtga gctccagttg cgtatctttg ttaccttttt ctttttaagt
      601 tactacggaa gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa agaaattatg
      661 gtcaagaata attgtcagat gaatttgttt caggaagaaa gcccatatga gcaatctttc
      721 tttattatgt attttgttgt gcatctcatg agagaatccc aaggaactcc atttgaatgg
      781 aaagctggag actcttatgt acagttgata aatatcgata aaaaagactt cttaatcttg
      841 aaaaatctct taggaaaatt gaagtacagc agctatataa aagtagtgaa ctattttgtc
      901 aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga gcagatacga
      961 aaaaatatgt cctactgttc gttttcatcc gctgattgca aagaaaaaaa cgaagaatat
     1021 gttgttcaat tattaacgca aatttatttt ttctcccgta ttttcccata cgatacgact
     1081 catttgacga tccgtgcaac ttactttgca cagcagatgc aacaccgcca gatacgtcta
     1141 tatgctggat ttcgtgcaga acttcaattt tattccactc tactgaatca gaatctgatt
     1201 tattatttat ctagctatat cttttggatg cttatcggta atcctgaaat tcaccattat
     1261 acttctgata aaaaaatact gattatcagc gacttaagtc ttcgacatag tcaatatatt
     1321 gaagaatata tcagtgatat tcttgctgtg cataaaattc actctgagac aactgctatt
     1381 actgaagatc agctgtcgaa atacaatctt ctcgagtatg atctgatcgt tacgaatcaa
     1441 cccattttga atgcccatgt tcctcatata ttgattgatg atagtgtgtc attcgcaaat
     1501 gaagaagaac tcatccgctt attcgagtta tgaataatca aaaaacagct atgtataaac
     1561 caccctgata aaaatttcag gacaaataca catagctgtt tctttttatt gtattccttc
     1621 agcaatccaa cgagatttat agcgaaagtc ttttaacaac tcttcttttg tccaattatt
     1681 tgcaatgaga tccgtcaaag tgttcattac gagggtcatg gaaaagtata ggaaactaac
     1741 tgcttcttcc gacgattgaa tggagaaatg attgattctt aataattcca gccatttttc
     1801 gttcattgga gaatcagccg atagttctct acgtttggtt gtctgctgat catttcgctg
     1861 ggttaacaag tataaccttt gccaatagat actttcgtga tctaagtcgc agcaccactc
     1921 taaatatttt tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga attttaagat
     1981 atcttgatgg actatgtgag aactgctttt gatcgtatac aaataagcat cttccaaatc
     2041 ttgaaaatat ttataaaaag caccgcgcga catccccatc ttgtcaacga tttctgagac
     2101 tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt aataggtcat cgattctctg
     2161 tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc atccacatct ctcctttatc
     2221 tctgattata cgtggatagc ccttcattgt catgaaggat tcattccttc tttttctctt
     2281 cttctgtcaa gtacccatct tccattcgat aaacgcgatc actccattga atcatacgga
     2341 catcatgagt aaccataata gtcgcttttt gtttctcatg agcttcttta actagtaatt
     2401 tgacgacttc atatgcatga tctgtatcca aactagctgt tggttcgtct gctaagatca
     2461 agcttggctc attatataaa gcgcgagcaa ttgccactct ctgtctttct ccaccagaaa
     2521 ggtctttagg ataactattt ttaagtgact ggatatctaa agattgcagc aaggaatcga
     2581 tttttgttga atcttctttt tttgaagtaa cttggtcaac tagttcaaat tgttccttta
     2641 ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa tcctatttct ttaaagcgta
     2701 aatctgctcg ttttttttct ggaagctcag ttagtgagtg accgttgatc actatttctc
     2761 cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa agtactttta cctgaaccag
     2821 aaggaccaat gatgctgacg aattctcctt cctttacata aaaatcaatt gctttcaaag
     2881 ctgagacctt tgtatgacca gaaccaaatg ttttggtaac ttttttcatc gttaatattt
     2941 ttttcatttt tattaacctc ctattgctgt aattgggtct actttggtta tcgtacgtat
     3001 cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc ccactatata gcaaccattg
     3061 tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc agactagtca aataagcaaa
     3121 caaaagagca agcagaacgc ctaccacccc aacaataaag gactgtgcta ctaaagattt
     3181 agcgatgaag aaatttttga tcccttgtgc tttcattact ccaaaaatag cagtcttctg
     3241 aagtgtcatc acgtacatga aaataccgac cacggcagca accactaaga acaaaaagta
     3301 tatcatcgca tttaaggtca tattttgggc agaatagcca ggaatacttt caatgaatgt
     3361 cccaatagtt aataactgta gacccttcga tgctttttct ttcgaaattt tagctgaatc
     3421 ctttattacg atcccattga tcggctgttc ttcttcagaa gcaaacggct gttgtccata
     3481 cttcaaagca gtccacgtat ctagggacgc atagatgacg ggagaaactg tgtaataagt
     3541 ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt tgatcgtatc
     3601 tccaatttta tagcctgaat cagccagatt ttgagagatg atgatttcat tctttgtttt
     3661 aaattcatgc cctttcgtga gttttggtaa taaaaatgct tctttcgttg tgccgaaaac
     3721 tgtaacattt tctttgtttt ttcctttgat tgctccgctg tacaatccga ttggtgcttt
     3781 ctctcctcct tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg caaatacttg
     3841 atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg cttttttaaa
     3901 ctcttctgaa agtccattcg ctagtcctga caacatgaat acgacatacg caatcaagaa
     3961 cataataccg acgatcaagc catatcgtaa tttagaatag cgaatttctt tgatagctag
     4021 aaacatttgt ttttcctccc tatgatagat tgatagtgac aaaatgtcac ttgttgaata
     4081 gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt tttcttatca ataaattgtc
     4141 taagacatct ttcgataaaa ccttaacatg agtcataaaa aatttattta agccttattt
     4201 atgctttcca tgataaacta aagtgaccaa atcaatgagg tgggaaaatg attaaactat
     4261 tagtagtaga agacgataaa gtattatcca ataatataaa agaaatactc gctgcaatcg
     4321 gcgagataac acaagtttat gatggtagtg aaggactgtt cgagttggaa agtggagttt
     4381 atgatcttgc aatcttggat ttgatgatgc cggaaatgaa tggatatgaa gtgttagcaa
     4441 aaatacggaa agaaaaaata cagactccag tattgatttt gactgcaaaa gatagtttgg
     4501 aagataagat tgaaggattt gaacgaggtg cagatgatta tctaacaaaa cctttctatc
     4561 gagaagaact gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt ctctttgatg
     4621 aaaatgttct gaagtataag gatatcagtt gtgatttaaa aagcaatgaa gtcttgtata
     4681 aagaacaagt tttgcccata caaggaaaag agtttgaatt gctcgtttat tttttacaaa
     4741 ataagggaat cattttgaca aaagagcaga tatttgaccg gatctgggga tttgattctg
     4801 atacgacgat tacagttatt gaagtttata tgagcaactt acgaaaacat ctgcgaagta
     4861 ctggattgga taaagaaatc aaaaccttac gtaatgtcgg ttatatactg caaagggtgt
     4921 gatgcaatga atggagatat tacacgaaag caacgtagtc gcttttttgc gacaaatgta
     4981 gcagcatttg cttttatctt ttttcttttg ggattcatta ctttacagat attaaaccaa
     5041 tctgcataca gagaaacaga tctgtcatta gagaaaataa attttgattc tcgaacagta
     5101 caattagaga tacaaagata tcagcaaggc gatccctttt taaacccgac tcaaacggaa
     5161 caaaacagaa caaacatgga acagccatta atggaaccat caaatgaccg ttttaacact
     5221 caagtgatct tgtggtctaa aaatggtgag atattgaata aagcagctat tggtgggcgt
     5281 ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc tgaataccat ccaaaatatt
     5341 gaattaaatg aagacagtgg ctcatctgcc ctctctttcc gttcgattac gaaaaaagcg
     5401 gagacgaatc aagatggcgt tgcctacatc caaattttag aaaatacaaa tcaaatcaag
     5461 aattcattag cgacttttcg cacgattatg attttgtgta tgattgtttt ttggattctc
     5521 tctattggta tcagttatta cttgtctagt ctaagcatgc gccctattat tctttcttgg
     5581 agaagacaaa aagaatttgt tgagaatgct tctcatgaat tgcgcacacc gttaacgatt
     5641 attcaaaata gtttggaaca tctttttacc aagcctaatc atactattat tgaagaatcc
     5701 gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca ctggattgac ttcggatttg
     5761 ctgacaatcg ctcggaatga ttctgatcag cagctgcttt caaagcaaat gatcaataca
     5821 caagaatata tcgaacaact tgttaaacca tttcaagaga tggccatcat agatggaaaa
     5881 gagtttattc tccataacga tgcaacatta gaagtaccaa ttgatgaaaa aaaaatccat
     5941 caagttttgg ttattttgct ggataatgca ttaaaatata cgaatccaga agataagatc
     6001 attttggatt catctgttaa ccataaagaa tggctgattc acgtaaagaa tactggccct
     6061 tctatcagtg aagaagatca agcaaggatt tttgatcgat tttatcggga agatcaatct
     6121 cgggcgaaag agactggcgg atacgggctt ggcttggcaa ttgcaaaaca ggtaatcgaa
     6181 cagcatcatg gacagatttc tgtatccaat tatcttccac aaggagtcga ttttaaagtc
     6241 caattgccgt tgaagcctaa aaaataatgc ttatccctaa aaaagggctg tgacaaaaat
     6301 cttgtcacag cccttttttg ttgcgaataa acggtgttcc agaagtagct tcttcggaaa
     6361 taagccgaaa tttctttaag ctgaccactt ctgtcacaac ctctttagct tacgcttacg
     6421 attttttatt gcggtcttca atgtgtacat caccgcttgt gtcaatatcc aattcttctc
     6481 ggctaacatc ttctgtcact ttttcaacat cttcatgttc ttctttatga atcccaactt
     6541 cttcacgaat aactgtatgt ttgcttacat ctacttgctc ttctttgatt gggatagtaa
     6601 tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt actggtttgc
     6661 gttcgatgac gatttcttca cgtttgaccg gtacttctac cgtttcagta tcgttgacca
     6721 catgtttgtg gatgtcgact tctccagttg tcacatcatg tgtatttaca tctaattttt
     6781 cttcttttag ttgaattgta tcatcatcta gatttggttt gcgatcgatg tcttcagtct
     6841 tgcttgtacc cattgtatca gtctcgccag gtacgtccat cggatcagtt cctggtgtca
     6901 tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc attggatctg
     6961 ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc tgggctaaca gtatcttgtg
     7021 tatccattgt atcttctgtt gtttctggct gacgatagcc ttttactgtg atgacaaatt
     7081 ttccatcaga aatatctcct ctatattgat ataaaggatc attttctggt gtagccgtct
     7141 cagaatcata ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa gaacggtctt
     7201 cttcaccatc catccgattt tcatctgttt caacatctat accagacaat ccgtcaaatc
     7261 ctagtctaga tgctgcttca tcagtagtat aaataacgat atcatctcgt tcataccctt
     7321 cgttacgcaa ccgttcaatt actgataatg cttcttccct cgtactatag cttccgatga
     7381 ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa atagatttat gattggtata
     7441 ttgttactta taaatttatt ctataggaga cgattgtttt ctacaaatat ttagattgga
     7501 atttgattaa aacaatttaa ttgaaatttg aaaacattta tgttaatgaa agtcatattc
     7561 taataaaatg tcatttgttt ctatatgaat ttttatgaaa aatttcatat aatttccact
     7621 cgttgtgatg gtttaattta tccaatccat caataagcaa acaacttata taaaaaagga
     7681 ttaattctca atataattgc tcctaccata gaataaatct tcatgtaaag taatgaagta
     7741 ccaactattt ctaatacttc tttaagaatt atcccaacaa tatcgttaga caaaccatta
     7801 ttgaataaat ggagagccaa tcgattctga aaaaatgttt tctaattttt cggaaataaa
     7861 agaaagagcc tcctgccatt agaacgaacc aaatccattt cattgctcat gaagtaacca
     7921 catagctaaa aagaggataa atcaattaaa agatcaaaag aatgactgca ctaataaaat
     7981 aactaaaaat gtatcttctt acttcatatc ttttagctag tacttagtta aaaatttgct
     8041 tcctaagatc aagacaaact gtctatcttc atttgatgaa atgcggtaaa atatagaaaa
     8101 tgaacactat tggagggaat ttgtaatgga cagcaaaact agaaaacaac tgcaagaaaa
     8161 gttaaagcaa cgtacacact tgctcccttc cgtctttcaa cagtttttag aagaaaatcg
     8221 ttcttccctt tcattaggca gtcgctatga atacgcaaaa gattttcatc tgtttactga
     8281 ttatttgaga aaagtatttt ctgaagaagt ggatgatcag ctgattatcc atttagaaaa
     8341 acaagattat cagaatttct tagctaaaat ctatcgacat actcgttcgt tccaaactac
     8401 agcgaaccaa gaaacggagc aattgtttga aaatagctat tacgggattt caaggaagca
     8461 atattcatta aatcattttt tacgattttt gtatcaaaaa ggactgattg aagaagagat
     8521 ttctcttttg ggtacaagtt taccggaaac aaccaaagca gctccgcgat atatcgatgc
     8581 agaacttttc aaagattttg agcatttgtt tatcccgatt gaaggctttc aaacaagcag
     8641 agaagctagc tttgaagaaa agaatagacc gaggaacctt gcaattactg ccattcttct
     8701 gtattgcgga ttaaagatcc atgaagtagt agaacttaaa cgtaaagatg tatctttgac
     8761 caagcagatt cttaatctga atagaaaaga aaacagactg aataaagtac caataccaaa
     8821 agaagcgatg ccatttctgg agaactattt taagttaaca gcacaaaata cggacgaaaa
     8881 ttcttttgtt tttcgttcct cacatgatca gcaaatttct cctcggacta ttcgccaaat
     8941 tttgagcaaa attacagtat ataaaaacgt atcgccagaa ctatgcagaa aaacatatcg
     9001 ttattatgca gaactatatt tagatgatgc tgacgcggta aattatttat gtggtaatcg
     9061 atacctctca tcccattctt ttcaagatat ctgggaaaag atggttgatt tttcttatca
     9121 cgaattagcg gcattcgttc gggtttcttc catataaaac aaatgtatga tctttttttc
     9181 acaataaact atgttattat ataaaggatt atgttacgat tatcttgaat ttgtatatta
     9241 cttaacaaaa gataatggag tgtgaatgat ggagaccata acgtattccc ctttgaactt
     9301 atttacgaat tttcaagaag ctgcagctaa cttttccgag accccgatta tctttgatga
     9361 gccactttca gctttcccag aattaggact tgaaacaact tacaaaaaat gctgcgaaag
     9421 tgtgttgaaa cgggcttatc aattggctca tcttggagtg aaatccggag acaaagttat
     9481 tatttataag agttccgctt ttgacacata ccttctagct gtttctgtgt cttaccttgg
     9541 tgcagtgcct gtcatgactt cttatcatct accaacaaca acaatggaag tatttatcga
     9601 tcgtttagaa gaccctttca ttttatttga tgatgagaca aaaaagcgag tgagagaaat
     9661 ttcaaatgga acaaaatcaa aacagattc
//
LOCUS       DACCQI010000104         9555 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCQI010000104 DACCQI010000000
VERSION     DACCQI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.108"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..344
                     /locus_tag="IVO76_002230"
     CDS             <1..344
                     /locus_tag="IVO76_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430169.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
     gene            425..1045
                     /locus_tag="IVO76_002231"
     CDS             425..1045
                     /locus_tag="IVO76_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430170.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            1746..2030
                     /locus_tag="IVO76_002232"
     CDS             1746..2030
                     /locus_tag="IVO76_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7430171.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2033..2950
                     /locus_tag="IVO76_002233"
     CDS             2033..2950
                     /locus_tag="IVO76_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7430172.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            2943..3725
                     /locus_tag="IVO76_002234"
     CDS             2943..3725
                     /locus_tag="IVO76_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7430173.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3726..4406
                     /locus_tag="IVO76_002235"
     CDS             3726..4406
                     /locus_tag="IVO76_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7430174.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4399..5295
                     /locus_tag="IVO76_002236"
     CDS             4399..5295
                     /locus_tag="IVO76_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7430175.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5490..7139
                     /locus_tag="IVO76_002237"
     CDS             5490..7139
                     /locus_tag="IVO76_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7430176.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7733..8668
                     /locus_tag="IVO76_002238"
     CDS             7733..8668
                     /locus_tag="IVO76_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7430177.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8661..9431
                     /locus_tag="IVO76_002239"
     CDS             8661..9431
                     /locus_tag="IVO76_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7430178.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         3056 a         1584 c         2040 g         2875 t
ORIGIN      
        1 caaatataaa aactagtacg aaattaattt gtaaattatt taagcttgat aaaataagca
       61 atatcgatga tgtaaagtcc aagatattaa caagaaaagt aagcgaacca gtaaagctaa
      121 cggcacaaga taaaaaaaat ggaattgttc tttatggtga tgatgcaaag tataactata
      181 attgggatac ctcctcagaa gctgaggtat taagtattcc tactaccgat acttcagggg
      241 aaaaagtcac ctttaaaata aattttataa cggcaatttc taagttggag tatgggaagc
      301 aaaaaaagag agataacaat gatcaatatc tacctaaaaa ataacgaatt aatgatgcat
      361 atgttaaagt tgtttttttg aattaaacaa ctcaatttat tcagttatat gttcatcgca
      421 gcaagtgaac attaatcgag ttataaaatt aataggagct aaaaatataa ataaagttcg
      481 atcagaattt atttttccag aagatttttt taactggttt ttttatcaat tcagtgaatg
      541 caagcgactc ttaggtgaga attattactt aaaaggaatc ggcggattta tggggaatgt
      601 ggctgatgaa caaaatatta taaaaggaat atctgaacaa acatcagagc ttacagtaac
      661 taaagcattt ataagtaatg gtgaaagttt gaaaaatgta actgcgcgaa ttagaaaaga
      721 aaatgaagta gacattgttt ttggaattga ccatgaatgt aattcagtga tatatgtaac
      781 acaatcagca aatttaattc tttttgagaa tggttgcaag gacgatttta tggtggtcta
      841 tttgtacgat atactaattc caaaacttcg ttccttatat atgtcttctg aaagtatttt
      901 tgtgagtgaa caaaagggat ctttctcgaa aaaaataggt attgaagtaa ttgaatcgat
      961 tatgtgtcat aatcatatta cgctagaaga attagaaatt ttgatagggc aaaaaaataa
     1021 taacacgaaa atacaagttt cttagaaatc attttttatt gagatatcta gataaatgat
     1081 ttattcttca atcaaccaac tcgccaagct gaggtaatgt tttgaggtaa tgataacata
     1141 caacttttta taccctagta aaactctata aacaaagact tattaatttc gcatgaaata
     1201 cattcctgta aaatgcaata aaatagagtt ttgtttaacc aggtcttaag aaagcccgta
     1261 aagcttcaca gttctcaaaa cgttaattgc gattatcgtt cgagttcacg agttacgaga
     1321 atcgtactgg gcgaagcaat gctgagcgca gttaccttaa tcctaacttt tctggcactt
     1381 tccacaattt ggaaagtgcc ttttttgtgt ttttagctat gttaataggt aggattcttg
     1441 gtctataata tcattcgatc tataaacaaa cgggaaaaag ataaagtatc atcaaacaat
     1501 attatttatc cgcttctgag gttgtgacag aagtggtcag cttcaagaaa taagaaggaa
     1561 tttacaaaaa ttgcttctca tgtttgataa atattgtgac ttgtgaccga ggagttggca
     1621 ggagaacacc gtttattcgg aagaagggat tgtgaaaagt cttttgtcat aacctcttat
     1681 ttggtagcta attgtaaatc ccaaacggca tgtattattt ttcataagtc cgtttttaag
     1741 gattaatgtt ttcttttgct ttttatatgg tctggcttca gcgagaagtt ccaacgcata
     1801 tacaaggacg tctgatgtca acgataacaa cgattaccat ggtggccagt ccacttggat
     1861 tagcactgtt tggtgcgctc tttgattggc agacaactca accacgtctg cgagacagtc
     1921 tgatttttct tgaaattcaa ttggaaaatt tacactttct ttatacattt ctggctgctt
     1981 tctttatacc cgtcaactat acttcactta tcaataagaa agaaggataa gtatggattt
     2041 tggaaatgaa acggtcttga aagcaactgg tataaccaaa aaatacggtg ctgccaaagc
     2101 attagataaa gtatcgatcg aaatcaaacg tggaatgatc tatggtttga ttggggagaa
     2161 tggagcgggt aagtctacct ttatgagaac tattatgggt ttgatctcga ttgatgaagg
     2221 ttcgatcgaa ttatttggaa cgacggattt gcaggcagca agacgacgta tgggtcaatc
     2281 gattgaaacc cctgctcttt atccagaatt aactgcaaga gataacctac gtatccaagc
     2341 agcgaacggc ggtgtgtcgg atagagaaat cgaagacctg ctgaaaatga tgcgattgga
     2401 aaatacaggg aaaaagaaag caaagaattt ctctttaggt atgcgccagc gtttagctat
     2461 tgcaaatgca ctgatcacga atccagaatt tttgattttg gatgaaccga cgaatgggat
     2521 ggatccagca gggatggcag aaatgcgaga aatcatccaa cgtttggtaa aagaacgggg
     2581 catcaccgtc ttgctttcta gccacttgct agatgaattg tcacagatcg ccactcacta
     2641 cggtattttg catgaaggac acttgatcaa agaattatcg aaagaagaat tggcacaaga
     2701 atctcgtcaa tttataaaaa ttgacacttc ggcgacggaa caagcagtaa cggttctcga
     2761 ctctcttggt tatagggatt attttgtcca atctagccgt gttatccagc tttttgaagg
     2821 aattgaccaa gtagcagcca tcaatcaagc attggtagag gcaaaagttc ctgtggatgg
     2881 tatccaccta gttgggcaaa aactggaaga ttatttctta caattaactg gaggcaatcc
     2941 acatgtttaa tctattaact gctgaaaaaa taaaagtatg gaagagccgt aaaatgtggg
     3001 tctcactagg actgatggtc gtacttccgc ttttttattc ttggaacgaa tggtatatgc
     3061 atacaaaata cggcaatgaa ttaaaccaag caacggatac agtcatcaac ggtgcaacag
     3121 ggattttaat ggtggaaaaa atggctgcat ggattcttct ggctttcgca gcatttgcgt
     3181 gcttttatat tggggaagaa tttcaaaacg gtacgattcg caatacgtta tctttaggac
     3241 ggaatcgaat gacttattat gtgtcgaaac ttctcgttac gcttttgatc acaacacttg
     3301 gcgtggtttt gattacgggg ctagccatga ttgcttacac acttgctttc ggatttggtg
     3361 aagttgaggg aatcaaaaac tatggaaatt atgttctaaa agtatttcct gtattgcttc
     3421 tgctgatatt agcgacatta tctgttccag tggctttgac attcatcact agaagtacca
     3481 gtgtcagttt gcttctttct tttctttata tcatgggaac ggccttcgtt cccggtgttt
     3541 tcgcaaaaat caaaggcttg gaatttctaa cggagtggtt tacggaaacg tggctcatgt
     3601 atacggactt tgcgcaacaa gcaacttatt cgcaagcacc gaaaatgatt ctggtcagtc
     3661 tagtaacaat cgtggtatca tcagctctag gaatgtttat tttccaaaaa tctgacatta
     3721 agtaggtgtt cctgctggaa actattttga ttatcgaaga cgatgaagct gttcattctt
     3781 tactggagga ggttcttgaa cagagataca aacttcttga tgcttattca ggtacggaag
     3841 gaaaattatt gttaagcaca tatcctgtcg atttgatcct tctagatctc atgcttccag
     3901 ggctttcagg tgaagcattg ttagcagaaa tccgacagac atcgaatgtt ccgatcatcg
     3961 ttctttcagc aaaaagcgat caaagagata aagtttcctt gttggcagca ggagcggatg
     4021 actatgtgac gaaaccattt gatatcgaag aactgttgtt acgaatcggc atccaacttc
     4081 gtcatcagac ttctgtccaa gtaaaagatc tgtcgcaacg catctattac aaggaaatct
     4141 tactggacaa agagtcgagg gatgtaaatg tgaatggtgt ggctgtccat ttgacaggaa
     4201 gagaattcga tctgctgaag ctttttttag aacatccgaa aaaagtattt tcgcgagcaa
     4261 atctttatga aacagtttgg caagaaccat attttgatag tgagaaaaca gtgaatatgc
     4321 atatttccaa tcttcgaggg aaactagcaa aggcacatgc tgtgtatatt catacggtgt
     4381 ggggagtggg gtttagattt gattaagtgg ctgttgcttc tcgttgttat ctttatggga
     4441 agttttattt tcttcctgca aagggaaatc aagagattaa ctaagaaaat cgccactctt
     4501 tctgaacgac aagaatatgg tggtcgtctt tcagtcgaat ttcgagaaaa gaatctaatg
     4561 gacctagtag atgcactgaa tacatgggtg gatgaaaatg aacaaaaagg acaagcattt
     4621 caagaaatgg aagaaaacat cagactatcg atcgtcggcc tttctcatga tctcagaact
     4681 cctttgactg ctattaacgg ttatgtacaa ttactggaac aggtagagga tacggagaaa
     4741 cgggagaagt atttaacgat catcaaacaa tctgttgcaa gattactcag tatgacggat
     4801 cagttttatg atcttgctcg aatagagacg aagcaaaaag aaatggatct ccatacgctt
     4861 tctttttcta aagaggtgga agaagtattt ttctcttttt atgaacaatt ttctgagaaa
     4921 aatatcgagg tagcgttcgc tgaacactcg ataaatacaa ctgtattagc agatccgctc
     4981 atgttgctgc gagtgctgca aaatatcata cagaatttat tgcgttatgc agatggacaa
     5041 gcaaaggtga cattttcaaa agaggattca ttcattcgat taacagtggc caatcctatc
     5101 aaaccaggag cgcaaacctc tgttgaaaaa gtcttccagc gtttttatac cgaaaatacc
     5161 agccgcacaa atacggaatc tagcggaatc gggctttacc tgtcaaaaaa attggtggaa
     5221 ggaatgggag gagagatgac cgctagatta gacgggggca tcttctccat ctccgtcaag
     5281 ttgcggaggg tttaattggc tctaatttac ctgaagtgaa tgacaaaaaa ttgtatctcg
     5341 ttttaaagac atcttctttt ttggaaggtg tttttatttt ggtgtgattg atacgaatta
     5401 ggtaacgaag acagttattt ttgtaccccc ttttcaaata gcgcaacagt gctatactaa
     5461 aactaacaaa tcaaggaacg gagagggata tgggccaaag agaatttatc gttttaatta
     5521 ttattgcagt tgttatttta ttagttgttt tagacatttt ttcacgtttg aaagtaaagg
     5581 aaacagttcg tagtaattgg ggaaaaattc cttatcaacc gagatttgat aaagaagaaa
     5641 gtttgaagga tgcgtggctc acggagaaaa aattccgttc atgggatagt gaaatcgacg
     5701 atcttacttg gtatgacttg gatatgttcg aagtatttga agggatcaac agtacctatt
     5761 ctagtgtagg ttcagaagcg ttgtatcagc gtttacgtag ctttgatttt ggagaagatt
     5821 atcagttaga aaaactaatt gctttttatc aggaaaatcc tcaactgaga gaaagaatcc
     5881 aatatcaatt cgctcgttta ggaaaaaaag atcataattt tgcaaaacaa tatctagcgg
     5941 atggaaaaag taaaaagatt ggacgcgtag gtttatttat ttttcttggc ttgcttccgc
     6001 tggtgggtgt aggtttgctt cttttagggc aagtactagg aatctatcta gcaatcggtg
     6061 gcttagtatt caatacggtc tattatctag tgaagaaaca aacactagaa acagagctga
     6121 atagcatgag ttatttggtt tcaacgattt cagccgccaa tcagttagca aaaatgacga
     6181 caccacttca aaaagagctg aaacagcaaa ctaaaccatt aagagacatc cctaaattag
     6241 gtttttcatt tcgaatgaag agcgggagtg aagcagaatt gatttttgaa tatgtcaaca
     6301 tcatgttcat gctgccattt atctcctatc atttcgttct cactaaatta gaaaagcatt
     6361 caactgaagc catcaagctg tgggaattgc tgggagaatt ggaagttgca gcagccgttt
     6421 tgaattatcg cacctatatg ccgatcactt gccgaccaga gtttactgag ggcggtgtga
     6481 cagcgaaaga tatctatcat ccattgttaa aagaagcggt agttaatcca gtcaactggc
     6541 agaagaatac attagtcacg ggttccaatg catcaggaaa atctacgtat gtcaaaagta
     6601 tagccatcag ctgtattcta gcacagacga tccaaacagc gattgcagaa cagtttaccc
     6661 tgcaacctgg acatgtgttg acttccatgg caatcgaaga tgatttattt gaaggcgaca
     6721 gttattttgt ttcagaaatc aaatcgatca aacgtttgct ggaccaagtg gcttccaaag
     6781 aacgctgcta ctgcttcgta gatgagattt taaaaggaac aaatacgatc gaacggattg
     6841 cttcttccgc ttcagtcgtt cggtggcttg cggagtatcc atctttagca tttgtcgcca
     6901 cacatgatat cgaattgaca gagattttaa agaattattg tgagaatgtc cactttgaag
     6961 aacaagtaac agaaggaaaa ggaatcagtt ttgatttcaa attaaaagaa ggaccttcaa
     7021 ggacccgaaa tgcgattgcc ttgctgaaag ttttgaatta tcctaaatcc atagttgaac
     7081 aggcccaaaa agaaagtttg ttgttcgatg aacagcgtca gtggtatccg tttgattaac
     7141 tttatcaaac gaagcatgat gcgtgggtcc aaaaagacga ttatcaaaag aagtacattg
     7201 atgccaaaaa agtggcagaa ttcaatggga acgagtctgc tgtccagatg atcttggaag
     7261 atttagcaaa agacgataca aaataaacca cgctgtcttc cttctagcag cttttatcga
     7321 aaaaacttag catcagcgac gagacgcacg ttccatggca aagttctccg taaaacaaac
     7381 agcgaaaaca gaaattgaaa ataactattt ccattttatt tatttcggtt aaaataaaag
     7441 aaaaaagctt tcgataaaag gacggaggaa agcttaacat gaataaaaaa gacaagaacc
     7501 tcagttataa ggatcttcta tatgtcatcg tagtagttgc tgtcggtact tttatgattt
     7561 taaaagttcc agagttgttt agatagttat tctatcgaag atgatcatga actagaaaac
     7621 acatctcctc tttgttagca ggtgtgtttt tgtgtggaag ccaagttgtt ttttcatctg
     7681 ctgtattcca tgatacaatt aagaatcatg tctataagga gataggaaat aaatggcaaa
     7741 tgcgcttgaa ataaataatt tgaaaaagat ctatggaagt ggggtcgaag cattgcgggg
     7801 aatcgatctt tcggtgaaag aaggagattt ctatgcgcta ttagggccaa atggagctgg
     7861 gaagtctaca acgatcggga ttattacttc tttagtcaat aaaacatctg gaaaagtgaa
     7921 tgtcttcggt tatgatctgg acaaggatct agttcgagca aagcaacaga ttggattggt
     7981 tccacaagag ttcaatttta acccttttga aacggtccag caaatcgtcg tgaatcaagc
     8041 aggctactac ggtgtctctc gcaaagaagc gatgaaacgc agcgaaaaat atttgaagca
     8101 gtccaatctt tgggaaaaga gaaatgtccg tgcacgtatg ctttccgggg gaatgaagcg
     8161 aagactgatg attgctaggg cattgatgca tgaaccacgt ttgctgatct tggatgaacc
     8221 aacagcagga gttgacatcg agttgagacg cgagatgtgg gagtttctac gagaattgaa
     8281 cgaaaatggt acaacgatca ttttgacgac gcattatcta gaagaagctg aaatgctttg
     8341 tcgcaacatc ggaatcatcc aatcgggtga actgatcgag aatacaagta tgaaagagct
     8401 tctgtcaaaa ctgcaatttg aaacatttat ctttgattta gcgccatatg ataccaaacc
     8461 tgtgattgaa ggatatgctt gtacatttga agatgagcag acaatcgctg tcgaagttga
     8521 acgaaatcaa ggggtgaacg gaatcttcga tcagcttaca agacaaggaa tcaaagttct
     8581 ttctatgagg aataagtcga atcgtttaga agaactgttc ttgaaaatca cagaagaaaa
     8641 taatcatacg gaggagaaaa atgtttaatc tttattttac tgcattaaaa agtttagcta
     8701 tcaaggaaac gaaccgctat ttgcgtatat gggtccaaac actggttccg ccagtgatca
     8761 cgacatcctt gtattttgtt attttcggaa aaatgatcgg tgggcggatc ggtgacatgg
     8821 gtggtttttc atatatggaa ttcatcgttc ctggtttgat tatgatgtct gctatcacta
     8881 gttcgtatgc caatgtttct tcgtccttct tctctcaaaa atttcagaaa aatatcgaag
     8941 aaatcttggt agcacctgtt cctacgcatg tgattatttg gggctttgtg attggtggtg
     9001 tgggtcgcag tattctcgtc ggctctctcg taacgatcat ttccttgttc tttgtaccac
     9061 ttcatgtgta ttcatggttc atggtcatca ttacattatt gatgacggca atcttgtttt
     9121 cattagcagg actgctcaac gggatttttg ctcagtctta tgatgatgta tcgatcgtgc
     9181 ctacatttgt cttgcagccg ttaacttatc taggtggcgt tttctatgcc atctctatgt
     9241 taccgccctt ttggcaagct gtttcaaaag tcaatccgat cgtttatatg atctccggtt
     9301 tccgttacgg attccttgga acgatcgatg tgccagtagc aacatcgatg atcgtacttg
     9361 tgttattcat cgttgttttg tacgccgtta cttggtattt gatcaataaa ggaagaggat
     9421 tgagaagtta gaaaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa
     9481 cgggaaaata gttcctcata ttttgttgtt ttttgagcga ataccgaggg tcagctgatt
     9541 gaacaccgtt tagtg
//
LOCUS       DACCQI010000105         9329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCQI010000105 DACCQI010000000
VERSION     DACCQI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.109"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..537
                     /locus_tag="IVO76_002240"
     CDS             <1..537
                     /locus_tag="IVO76_002240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430179.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..2375
                     /locus_tag="IVO76_002241"
     CDS             564..2375
                     /locus_tag="IVO76_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7430180.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2436..3905
                     /locus_tag="IVO76_002242"
     CDS             2436..3905
                     /locus_tag="IVO76_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAP7430181.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3916..5925
                     /gene="topB"
                     /locus_tag="IVO76_002243"
     CDS             3916..5925
                     /gene="topB"
                     /locus_tag="IVO76_002243"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7430182.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKRSEGFI"
     gene            6271..6867
                     /locus_tag="IVO76_002244"
     CDS             6271..6867
                     /locus_tag="IVO76_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7430183.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            6880..7779
                     /locus_tag="IVO76_002245"
     CDS             6880..7779
                     /locus_tag="IVO76_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7430184.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            7782..7913
                     /gene="ssb"
                     /locus_tag="IVO76_002246"
     CDS             7782..7913
                     /gene="ssb"
                     /locus_tag="IVO76_002246"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7430185.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            7935..8267
                     /gene="ssb"
                     /locus_tag="IVO76_002247"
     CDS             7935..8267
                     /gene="ssb"
                     /locus_tag="IVO76_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7430186.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            8289..8546
                     /locus_tag="IVO76_002248"
     CDS             8289..8546
                     /locus_tag="IVO76_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430187.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(8705..8827)
                     /locus_tag="IVO76_002249"
     CDS             complement(8705..8827)
                     /locus_tag="IVO76_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP7430188.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            9017..9241
                     /locus_tag="IVO76_002250"
     CDS             9017..9241
                     /locus_tag="IVO76_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430189.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
BASE COUNT         3475 a         1571 c         1987 g         2296 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aatagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaaacttac tacacgattg
     1441 aaaaagtgtt tgatatcaca caaacacagt taaaacctga agactatccg aaaatctttc
     1501 cgaaccgtgt ctttgatttt caattggata aaaaaggaca gtcagaactt caagctggga
     1561 ttcaagcggt agccaacaat attggaatac aaattcgaga tatgacagaa agtgaagtct
     1621 atcaaagaga attaggacaa gcacgtgggg cgtatgttcg taacgaattt actggcgaag
     1681 aagaaatcgt catgaatact cgaaatactc ctacgcagca tttagctaca agtattcatg
     1741 aattagccca tgcaagaatg cacaaatttt cagaattaga taccgcaact aaagaattgc
     1801 aggcagaaat gacttcttat attgtgtgca aacattttgg aatggacacg ttggagaaag
     1861 ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat aaggaagctg
     1921 cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc attcaaacca
     1981 tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca gcagaagtga
     2041 agcaggagca taaaaatact gtagaaacgg cagaacaaaa gaatattgtt cctgttggtt
     2101 cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac
     2161 atttgaagga tgcggcgttt attgaagatc cacagcataa tcaagtaatt ttaaaaggaa
     2221 aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg ctaggaaatt
     2281 cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt
     2341 atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga ctatctgaaa
     2401 aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa atgagtctta
     2461 ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga atggctgtag
     2521 tcaataaagg acgagactat cgcttagaag accatgattc atttgttttt gatcgaagaa
     2581 aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa ttagctaaac
     2641 tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca gccactgatt
     2701 ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt gagacagaaa
     2761 agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg aattatctga
     2821 aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa gggttgattg
     2881 ctgaactaaa gccgaaacat gaacggcaaa attttttagt tggagacgac cgtttagatc
     2941 atgcggttgc ttttttatgg aaagatccac agacaggaga gacagtaggg gcaagttatc
     3001 aaggaacgat cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa cacattgaca
     3061 agaatcctac gccaaatcac ggatttaatt taaaaatagg cgatccgaag catttaaaat
     3121 tctttgaaag tagtattgat ctgttaagtt acgctgcatt gaatcgggag aaactacaag
     3181 atgcctggtt agtttcgatg gacgggttaa aacatcatgt aatcagtcat tacgttgaag
     3241 agtcgatttc tgaattaaga cgaaaacaaa catttcctca atcgattgaa atatgtgtgg
     3301 acaatgatcg tgcaggacat attttttatg aaaaagaaca gatgaaggga atcgttgatc
     3361 cttttaccaa taagaaaatc cgttgtgagc gaggaattcc aaatgattgg caagtaccta
     3421 aagaatacaa agctacttat gaagcagtcg caaaagaaat gagtgttgaa cctgaagcaa
     3481 ttatggccat acacaaaact gaaactaatt tacagctgac aaatcaattg gtgtctgctc
     3541 atgatgttca atctacgttt ggaaagatgt tggcgaaggg cgaaccagta gaaacaattg
     3601 atttgaaaga agcatgtacc acggtcgcga aggaactgaa agtatgtgaa cgcgcagatg
     3661 gaacatacaa ctttgatcgt ttttatagta gaaaagccaa cataaaagac gttaatgcag
     3721 gaatccttct ttcctataaa gcagaacaat actacaaagg ctataaaaag catgaacatg
     3781 aatttgtacc tgaagtgaaa aaggattgga atgatcaatt gaagcatgag atccagcaac
     3841 aagaaatcag aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa caagaaaggg
     3901 agtgaagtcg tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca agcgaaggct
     3961 tatgccgata gcttttctaa agctacccga aaagatgggt attttgagat ccaagatcga
     4021 ttgtttacag gagaaactgt gatcacgtat ggttttggtc atttagtcga gttagattcg
     4081 cccgatatgt acgatgaaaa atggaaacaa tgggcattag aacatttgcc tattttcccc
     4141 aatcaatacc actatcatgt tcctaaagat aagaaaaagc agtttagtgt cgtcaaacga
     4201 cagctgcagt cagctgatac gattgtgatc gcgactgata gcgatcgtga aggggagctg
     4261 attgcctggt caatcattca acaagcaggc gccaatcaag gaaaaacctt taaaagactt
     4321 tggatcaact ccttagaaaa agaagccatt tatcagggat tccaacagtt acgagacgca
     4381 gaagagacct atccaaaatt tgaagaagta caagcacgtc agatcgccga ttggttaatt
     4441 gggatgaacg gtagcccact gtatagttta ctcttgcagc aaaaaggcat accaggaagt
     4501 ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt gcaggaaaag
     4561 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
     4621 ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga agcatttgaa
     4681 gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat ccttcaagtc
     4741 gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag ccttcagtct
     4801 aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
     4861 tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac ggaaggggaa
     4921 tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc cgaagcgatt
     4981 ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt ccaggaacat
     5041 tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca actaagtcca
     5101 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
     5161 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt aaaggcaatc
     5221 gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag cgacggaaaa
     5281 gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt gacggtggat
     5341 ctacaagttc ttgaaaaaga aaccaagcca ccacaacctt ataccgaagg tacgttgatc
     5401 actgcaatga aaacagctgg gaaaaccgtg gatagtgaag aagcgcaatc catattgaaa
     5461 gaagtggaag gaatcggtac agaagcgaca cgggcgaata ttattgaaac cttgaaacaa
     5521 aaggaatata tcaaggtaga gaaaaataag ctcgtggtga cgaataaagg gattttgctt
     5581 tgccaagccg tggaaaaaga gccgctgctt acgagcgccg aaatgaccgc caaatgggaa
     5641 agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa ccacttttct agccaatatc
     5701 caaaaatttg tctctcactt attggaagtc gtaccagggc aaatccaatc cactgatttt
     5761 ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc aagaagccgc gcgtcattta
     5821 ggaatttgtc caaaatgcca ggaacaagaa gtcttactct atcaaaaggt ggcagcttgt
     5881 acgtctgaag cctgtgattt taaaaggtca gaagggttca tttgaagcaa cgattgtttt
     5941 aaaagaagac ttcactacag gctttgaatt ttctgaaaag aaaaaaacga acttcaagaa
     6001 aagaacacgc cgcactgcaa aataatggag ggttaatctt atgcgtaata ccacacacaa
     6061 acaacaagaa atcgaaaatc aaaaagccaa aattcgttca gaacaggaaa aaatgcgtgc
     6121 cagcaaacga agactgcagc aattgaagaa ccagcagagg attcaaaaag aaatcgaatt
     6181 agatcgataa aatcttccta actattgtat acgtataaac ttatgttata ctgtttttat
     6241 aagtttatac gtatacaaag gagggagaaa atgacaaaag caatggtata caagctgcta
     6301 gaagaattta atggaacttt ggctttgaag gatgccaaaa aagcgggcat ttctcctgta
     6361 acgatcaaac gtatggtaga tcgaggggag ctggatcgag agtatccagg ttttttcacc
     6421 ttacctggtc aatttcctga tgaattgttt atggcgcaaa aaaaatatga acgcggcatt
     6481 atttcacata ttaccgcgtt agatttgtat gatttaacag atatgatccc tagacagatc
     6541 gatctgactg taccttacgg ttatcacgtt tctgaaaaag gattgaagga attcgcagtt
     6601 gagctacact atacaaaagc ggaatggtat gaattaggta agattgaaat aaaaagtcgc
     6661 tatgggaatc ctattatagc ttatgatcca gaaagaactt tatgtgacat ttggaatccc
     6721 tggtataccg ttgaggatga aatcaaagta aaagcaatca aaaactatat ggagtcggac
     6781 agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt taccaacaga caaaacgatg
     6841 cgttcttata ttatggcttt aaattaaagg agaagataca tgacacctac gcagttaaaa
     6901 gcaaaagtga agaatatttc tagagaaaaa gaagttgatc cgcagcttgt tttaagacat
     6961 tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc cttatcgtga aaattttgtg
     7021 ttaaaaggcg gcttcttgat tggctcaaag tatggcattg aaaatcgcac aaccaaagat
     7081 attgatacga ctttacggga aatgaaagta accaaagaaa cattaacaac ggttctaaac
     7141 gaaatttttt caacgccgac gaaagaaggc attcaatttg agattcaagg aatgaaagaa
     7201 acgagagaag ccgattacta tccaggattt agcttacgtg tgctggcaca tttggaaaac
     7261 atgcggccag attttaaggt agatgtgact acaggtgact cgatctatcc tgcaacgatc
     7321 actcacagtc ataaattgat gtttgaggat cgaacgattc ctttggaatc ctatcctaca
     7381 gaacaaatta ttgctgaaaa attaagtgca acctttgatt ttttaacaga taattcgaga
     7441 ggaaaagatt tttatgatct ctatactatt cctaaaatgg agaagatcga tgagaagacc
     7501 ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa aaactgatcc gttaaagata
     7561 tactatgaaa aggatatgga tgtactgaaa aatagtcacg agctaaaaga tacctggaag
     7621 aaatatcaaa aaagcaatcc ttatgctgcg tcgatcacgt acgatgagac aatggattcg
     7681 attagtaatt tgatggaaaa agtgattcac gtcgaagaaa aagagcgaaa actagcttat
     7741 caaagacacc aacagatgaa tagagaaatg gagagataaa gatgattaat aatgtgacat
     7801 tagtaggacg cttaaccaaa gatcctgatt tacgttatac cgcaagtggt actgcagtag
     7861 cgacttttac cctagccgtc aaccgaaatt tcacgaatca aaacggcaat tgagaagccg
     7921 attttattaa ctgcgtgatt tggcgcaaac cagcagaaac aatggcgaca ttagctaaaa
     7981 aaggtagctt gattggtgta gttgggcgga ttcaaacgcg gacttacgac aaccagcaag
     8041 gacaacgtgt atatgtgact gaagttgtag cagataactt tcaactatta gaaagtaaag
     8101 cagcgacaga gagccgtgcg cacgctgatc aatcgtccac cagtccaagc accgccacct
     8161 ttgaacaacg gaatacagcg acgcctaaca acaatggatt gaacgcttca cagaatccat
     8221 ttggtggaca atcaatcgat attagcgatg atgatctacc gttttagttt gagaaagaag
     8281 gagtaagcat ggcaaaaaaa attagtctga cgatggatct attgataccc gtaattgcat
     8341 ttatagacgg gatttctttg ggcgtcactg aaaatccttt ggtaaataga attgcgaaag
     8401 gatcactatt tcttgtattc gtgtatcttg tctgttctta tcttttaaaa agagcaggaa
     8461 aggataattc cttttggcga ttgactaaag tcacagtagc agcgacagct gttatactta
     8521 cggctattct accgctttat atgtagagtt aacgggaaat cgttgaactt cttgcgaaat
     8581 attgctataa tgaacatgaa aaagaggagt tgcgctaaca acccctcatt aacagcaccg
     8641 attaaagaac ggtggcagaa tattagttat ttggaaataa ccgctgacct gctaaagtag
     8701 acggttattt ctttttgtca tttttaagca tatttacaat caaactaatc aaagcaatgg
     8761 taaacatacc aaaaccaaga atggtttgaa ttgtctcata tgcggacaaa aggcttactc
     8821 ctttcataga ttttagcact tacattcata agcaccacca cctttccaga agtcgccacc
     8881 gtcttcaaac tttactgtct tgactattat agcatatcta aaatcgaata aaaattctca
     8941 attttagctg tttttgctat aattttagcg aggtgatcgt gatggaacaa tatattggtt
     9001 tactcgataa attagggtga tcaagacaca gccattgctt gtgcgattta cattacgcac
     9061 gagtcatgaa ccaatcaatt gtgtagtggc caatatagaa atagcggata aattgctgat
     9121 tttggaagaa ggcaagtata ctatttcagt gacaggtcac ttcaataaac gaaatcaact
     9181 agtcattgaa tcaatgcaaa ttagaaaccc tgatcatttt acgagattaa tgggcatata
     9241 aataaaagaa gagctgcagt cctaaatgag gattgcagct ctttttgttt tacatagaaa
     9301 caagaaaaat gaaaaggcct ttttaggaa
//
LOCUS       DACCQI010000106         9260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCQI010000106 DACCQI010000000
VERSION     DACCQI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.110"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(152..1063)
                     /locus_tag="IVO76_002251"
     CDS             complement(152..1063)
                     /locus_tag="IVO76_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7430190.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(1466..2044)
                     /locus_tag="IVO76_002252"
     CDS             complement(1466..2044)
                     /locus_tag="IVO76_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7430191.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(2064..2732)
                     /locus_tag="IVO76_002253"
     CDS             complement(2064..2732)
                     /locus_tag="IVO76_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7430192.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(2747..3817)
                     /locus_tag="IVO76_002254"
     CDS             complement(2747..3817)
                     /locus_tag="IVO76_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7430193.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(3988..5352)
                     /locus_tag="IVO76_002255"
     CDS             complement(3988..5352)
                     /locus_tag="IVO76_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7430194.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(5349..6908)
                     /locus_tag="IVO76_002256"
     CDS             complement(5349..6908)
                     /locus_tag="IVO76_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7430195.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(6964..8739)
                     /locus_tag="IVO76_002257"
     CDS             complement(6964..8739)
                     /locus_tag="IVO76_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430196.1"
                     /translation="MSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGGNLN
                     PGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLTMGL
                     TEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQGGL
                     LSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIHYRL
                     YSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTNMYP
                     SFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQENV
                     TFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLSKIG
                     DTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTILVPK
                     QELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLTSLF
                     KNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLLEVN
                     SSTNAQPGATYQGMVTWELTAGPTS"
BASE COUNT         2660 a         1880 c         1584 g         3136 t
ORIGIN      
        1 ctgttttttg aaattgctcc ttgtgacgcg gagttaaacg gcaggctcac aacctcttcc
       61 ttaaacggtg ttcaaaagct tgcatctgcg ataataagcc cggccaaacg aaaaatatga
      121 ggagctattt ttgtttggct gggcttattt attactgaaa ataagtggtc aatgcttgtg
      181 tcaaacttgc agcaatctgc tgctgataac tgtctgttac taattccttt aaatcactat
      241 cattattcat ataacccatc tcgattaaaa tagaaggttg aagattttct cgaagaactt
      301 gatagttccc agtggagatt cctcttgaat ccagaggcag atcagtgaag gagtcagcaa
      361 ttgtctgtgc taatgtttcc tcttgtccat agtaataata tgtcgtgatt ccagttgctt
      421 cattcgcata ttccgtagag tctgcgtgta aactgataaa gacatctgcc tttttttctt
      481 cactgtagtc gcatatatct ccaagctgta ctaattcatc tgtagtatgg gtcaaataaa
      541 ccgttgctcc agcatcctct agcgcctttt ttacttttat ggctgtactt aatgttatat
      601 ctttttcttc tgtttgttca tcattgctta atgccccagt atcatttccg ccatgcccag
      661 gatctaatac aatcactgca tcagatagct cttccggttt tgtttgtata ttctttgtga
      721 ctttggttcc atcttcgagg gctaaatatc cagtatagcc atcatttgtc ttgatctgat
      781 aagcattttt tgtttcagaa agaaactccg ccagctctcc gccgtttata gttgcaagtg
      841 gggtagttag cgagtcatct gtatatagtg tacggtcatt ttttccgatt ttgattaatg
      901 tctcttgttc tgtatcatca aatgattcct ttgtctttgt aaccgtactg ttcgtactgc
      961 tgaccgtttg tccaactatc tttttttgac caaaaacatc tatttgaaaa cagataataa
     1021 tgataatgat tgcaaataca aaagctccaa ttattttttt cattcttgac tctcacctcg
     1081 tagatccaga ctaagggtca aaacttaaaa tcagcttaag tacgcaaaaa aggggctgta
     1141 aaagatataa ccgtagaaaa taacattact gctaaaatga tttttttcat ttttggcata
     1201 tcctgaaatc tccttttgac tattcgtggt gattcatcta cccaataaat agtttccttc
     1261 attctacaaa caattaattt cttcgttatt aaattaaaac acttcctgga acaccgacat
     1321 aataaataaa aatataccct gataatcatg agagaataac aaatttgtat ttatgaaaaa
     1381 ctacctatat aaaaaaaaca agatataacg atcttcgtca tatcttgctt cttcacctaa
     1441 ctatttttct ccctatataa cagctttacg cttctcctaa tacaccattc aaatataaat
     1501 cgatcaattg cgccacattt agcggttctt ccacaggtct tttcgaccga gtgatgtaag
     1561 gtgctaacac taagaaaaaa tgccttgctg cgatttcttt tgaaatccca tttcttagca
     1621 cagattggtt tgctgaaaag atttcttcaa aaggttcacg ataatttttc ttccatagaa
     1681 agaaaagatg atctcttgtt tcctttgaaa gattgctttc aagatcgtga agcatcagaa
     1741 acaaatcgac cagatgtgtc tcaatcaaaa actgcgccat ttttgtcaag gttacttgaa
     1801 atcccgtttc gcttgtttga acgatttctc tcaaagcttt tccaacatct tttaagtggt
     1861 tttctaaaac tgccgtatat agtttttctt tatcaccgaa ataatgatag agattgggtt
     1921 gggtgatccc aactttttgt gcgatctctc ttgttgaagt gccgagatat cccttggaca
     1981 taaacaagtc agtcgcttct ttcaaaattt tttgctgtgt atctttgttg tatcgatgga
     2041 ccatttttcc acctgctttt tggttattct ttttctttca atacaccatc ttccatcata
     2101 aatattctat cgcatttgtc aatcagccgt gtatcgtgag taaccatgat cgttgcttta
     2161 tttttgtctt ttgattcttt tgccaagata tcgactactt ctcgtgcctt ttctgaatcc
     2221 aaactagcag ttggttcatc tgctagaata atggacggat cattgtacaa tgcacgagcg
     2281 attgcgaccc tttgccgttc tccaccagat agttcttctg gatacttgtt gatcaactta
     2341 tccacgccaa gctgctcaaa taattctttt gcttcttttt gacgatgttc atgtttgatc
     2401 ttatctacta ataataactg cttttttact gtcagaaaag gaaccagatt ggaagcttgc
     2461 aaaataaagc caatttcttt aaaacgtagt ttaacgcgtt cactttcttt ttgattactg
     2521 aattcttgat cattgataaa tacagagcca ctgctaggcg tctgcaatcc gcctgccaaa
     2581 gtcaaaaaag tacttttacc tgaaccagaa ggaccgatga ttgcaacaaa ttccccttta
     2641 tcgatactca tggttgtttc tttcaatgct ttaactactt ggtcgccatc ttggaaactt
     2701 ttttctactt ctttaaattc tattgctttc atgctcttcc tccttcttat ccaattgcct
     2761 tcaacggatc gatttttacg attgtccgaa cagaaaatag tgcgcctaac acagctacta
     2821 ccagcatcaa caagctaatg acgccaaaaa acagccaatt gctttggaat ggaaccgcat
     2881 caggtagaac aagggatgtt cccactgttc ctaatagacc aagaagaata ccaacaaatg
     2941 ataaaatgaa tgtttgtgcc actacagaac gcgcaataaa tcctcctgtg atcccttgtg
     3001 ctttcattac accaaagata ttaatctttt gcatcgtcag tacataaatg aaaatcccga
     3061 tcacaatagc cgcaataacg atcaaaaatc cgatcataaa tccaaaagtc aatacttgcg
     3121 cattatatcc aggtaattca ttgatgaatt tagaaatttt gatctgttct aaatcttcag
     3181 gtaaattact gattttacct ctgacaacta ttgcgttaat ccgtgcattt tcacttgtat
     3241 cttctttttc gaagcggatc tcttggtaag cattgatggt tgtatagaga actggtgaga
     3301 cattgaattt ggcatgatcc gtaaagccag ttagtttaaa cgtcttgtca ctaccagata
     3361 acttaactgt atctcctacg gctagaccat attcttcttt caaactaata tcgcctactg
     3421 cctcatcatc gttatcaaaa gcttttcctt caacaagatt gggcatgatg aattgatttt
     3481 tatcgatacc gaagaagcta acattgattt ttccttcttc tgtactgtct ttactagtca
     3541 cgactccagg tgtctgggca agatacgcca ctttcccacc ttcgacttct tctgctatct
     3601 tcttagtaat catggacatc cctagatttg aatttgcatc tttcgaaagc acaatcgaat
     3661 ctgcctccca cttgtcgact gctgttcgat tatcttgagc taagccataa gctaacccag
     3721 tcaaaaaaaa taccaggtag gcgatcaaaa acatcacccc tgcaactaac gcataacgta
     3781 actttgaatg cataatttca tttaatgcta aaaacattta actcctcctc ctgaaataat
     3841 cattttatac aacaatttta tcacttgata aatttttggc gaatattatg ctcaaaaaat
     3901 tcttccgcta tttcattata tgaatttttt attctcgatt gattaatatt tattatatcc
     3961 ccacacaata aaagaccgtg atatttctca cagtctttta tctactctat tgctatttgc
     4021 tctcttagct agtactttaa aagctaactc attgaggtag tgaaaacgag aggtgtgttc
     4081 agacagaaat ccaaaatcct caactgctag ctgtggaatc gtagttttgt gtttcacgat
     4141 atccgcaagc aggttcaaat ctgataataa gaattcttgg cttatcagct gtccgccaat
     4201 tatttctaat gtatccggat ggtagatcag ctttgcattt actgttttgt tttcttcatc
     4261 gtattgtgga aatagatcaa attctcctga acacgaaccg gtattttgtt ccaagaatgg
     4321 cgcttcatct tctgtcagcc ctacagttcc taaaaagtag tctataatga ctgctgctgt
     4381 tatcttttgt ctgaggtcgt aagcggttgg ctgaccactc aaagttagcg ctactgctct
     4441 tgccatatgt acagctcgag taactaaagg taaaaagaga tgccgtttat ttttgtcaaa
     4501 actgactggg acgatatctc caatcgcata gatgtccgga tcacttgtct gcagatattc
     4561 attgactatc acagtctcat cctcatacaa agccactttt tcttttaaca atgtcgtatt
     4621 aggacgagtt tgggcagaaa aaatgaccat atctgccttt attgcttgag atagaagttg
     4681 tacggatact actttctctt ctgatttttt ccaatcgatc aagaattcat ttggataaaa
     4741 attgatttgc ttagtttgca gctgattttg aaaactgttg atcatatcct tatcgaaata
     4801 tcttgataaa acattcggca tataatcaac tagatgaata atgtatcctt tatttctcaa
     4861 agcatctgct aattccaatc caatataacc agcaccgaag atcactactt cttttgcttc
     4921 ttttaaagcg gcaagtactt gcttagattg cactagattt ttataggtgt agacattttc
     4981 agcttcaatt tgtttataca aggtgacagg agaggaacct gttgctacga ttaatttatc
     5041 ataggttatg gtctcgtttt ttccttcttt ggatgcgagg atgagctgat gtttgtctgg
     5101 atcaagttca gttactctcc attcggtgat cagttttgcc ccactttctt gaagagtccg
     5161 catgttatgg gcaacttcag ctaaactacg tatctttcct tttaaataaa ggttgattcc
     5221 atttgaaaca tagcctagtc catctttatg atcttcatcg atcagtaaaa cttctgcttt
     5281 cggttgaagt tttcttagat ttagggcagc tgtgatgcct gcatgggaag caccaatgat
     5341 cacgattttc atgttttatt catccttaaa ttcaattttt ctaaataaca ttcttttaaa
     5401 aattggttca aataggggaa atcataatta tattcgttgc tagtgaatac aaaaacttta
     5461 gcggacttct gtgacgggta ctctttcaat tggctagtca aaatgatatc atactgcttt
     5521 tctttctcgt attcgactaa atcatatttg atcgtattca gcaattgttc ctggatgatt
     5581 tctttcacga tatccgccat tagcctttca taaggaagat cataagcaat ttgcagacgt
     5641 aattctaact gcttgatcag cgtccgcaaa atagatgcat agcgagcaaa taattcaaag
     5701 cgggcatttt cctcgtctac ttcagtaaaa tcaacaatag caaatgtttg tctgaccaat
     5761 ctttcacata aaaccaggaa ttgtttatct tctacttcag ttaaccgctg ttttaatcct
     5821 tcttggccaa aaatattcat ccatcctcta aaataggcaa aatgagcgtg attttgcatc
     5881 actgtcaacg caatcttttg cgaaatctct tctggaaaaa cataaaaaga gaaatattct
     5941 tctatcgatt gtttaaacgt tcgattcaat tgagctacta cgtcgtcttt gtatttttcg
     6001 cttatcaaaa actcctggac atgcgagtta tgtgtatcta atgtaaagtt actgatcatg
     6061 aaaagataaa aaaccaacgt ttcattttga ttccaaggaa aggcatattg aaagaagtaa
     6121 cgggtcaaaa atgtatggag gtccaataaa aattgtgtcg tttgaaacaa tccaacagga
     6181 agattgctat aatctggttt ggattttttc gtacagcgca ttcgcttctt cataatggaa
     6241 agccatattt caaatttcaa tcgtccttct tctgtaatag taaaactaaa ctctttttgc
     6301 agcagatcta aaaaagtgtt tcctgataaa tcactgtatt cttttttgtt ttcaagataa
     6361 ggacgtccaa accaaaataa ttggaaataa aaataacaaa tttgactttc tggaccggtc
     6421 agccgcccgc tttttatttt cagatgaaac tcatttaaag aatcatttaa ttccttcact
     6481 ctgcgagaca aagtagccga actgatatta agttccatga caagtttctg ccgatcaaat
     6541 gagccatttt ccaacagata aagaagtatc tgatattttg tagaatagcg caagtagcaa
     6601 tgataataaa catcgactgc tgctatattt ccatgtctga ttaataaaat attcccttct
     6661 tggataacaa tttccaattg tttgatattt tccaagttag cttgtaaata aaacagatct
     6721 tctttcaagg tagcccgatc aactgataag cgggtaagta cgttattaac ggaaatctct
     6781 cctttttcct ctaataaaag ttggaaaagt ttgatttgcc gttgttcatt tttttcaagc
     6841 aaactgaaaa gttccaagtc cactcctcca agcatttctg attttaacag cagaagtttc
     6901 cttctcatcc ttgaaaaaga ggatctgtga cagttatcac agatccatac aaattctaat
     6961 cacttagctg gttggcccag ctgtcagttc ccatgtgacc atgccttgat acgttgcgcc
     7021 aggttgagca ttcgtactgc tatttacctc taagagtaaa ccatcggtat cgggaaagtc
     7081 aacgagaacc tctcctttag ctgttccatc actctcataa atcaacgtgt tttgaccttg
     7141 ctgaatcaat tgttttgatc cagaatgatt gaagtataat tttgttccgc ttaattcttg
     7201 accttgatca ttcttaaata aagaagtcaa tgtccctgtc agttgccaag gagacgtgct
     7261 tggaagccga gtatcccgca catcaaaaga aaaatcgcct tttgcttcat aataacttgt
     7321 cttcaacggt ttggggagat cgttaccaaa tgtaaaatca tctggtacat taactaatgt
     7381 taattcttgt ttggggacaa gaattgtttt agcagatgtt tggacaactg ggttaggaag
     7441 cttagcacca tctgctaatg taatattgct ggaaagtaca gctgcctttg tcagtatgct
     7501 gacggattgt acagaagtat ctacagtatt taattgatag ttgtaagtaa gatgaatggt
     7561 atctcctatt ttgctcaata gatctgctgg gacagaaact ccttgttgaa tgccggtatc
     7621 tgcgatagtt gtaccattta attttaagga accattgata taagtagtat tttcaggaag
     7681 tgagatcgtg atttttttgt cggttatcgc atccccagat tttgttaacg tgaatgtcac
     7741 attttcttga atcgtatctc ctgatagagc agacagggga tctcctgtct caggttgtct
     7801 gctaaggttt tttagctgac tatctgcact cacagatgga aaattagcca ctgttatttg
     7861 aggaagagtg gtgattccag cttgttcaga ctgccatttg tacgcaaaag acggatacat
     7921 gttagtaagt acatcgtcca ccaagcgata atcaaaataa tattgccctg aagccaatgc
     7981 aggtagtgta gaagtagctg tatttacacc cccgttattt gattggatcg tttggaaaag
     8041 ttgatctgtc catcctgtcg ctaatttttg ggttcctttc gaataaagcc tgtaatggat
     8101 cgaacccttt tttagttcag ttgctgacca attggccgtg acttggtacg atgcagaaga
     8161 aaggagcttt ccccactgac tatcggctgt taagtttgtt ggtaaaaccg ttccgtctag
     8221 cttgtactgc tccaatacct ttaaagcaac aggagaaagc aatccgcctt gcgcatcttt
     8281 cccactgtac acgatattgg ctgtctgatt caatccactt gtgcttgctg gagtatgagt
     8341 cagtattgct ccttgtgtaa cgttttttcc tgtaaaatag ttgactacgc gatcaaagcg
     8401 actaaatggc aactgatcaa aacgtccttc agtcagcccc atcgtcaatg tccgactatt
     8461 ggtagagcct ggaaggttct ggttatttcc atccacgtat tctgtggtat cagcaaatgt
     8521 attatatttt cctgttgaat cacttgttga gatgtaacta ttccacgtat ttatctctgc
     8581 acttgcagaa aatgcatatg ctcctggatt caagtttccg ccagttaatt taggataaaa
     8641 tctacctgca gctggagcta aaatcgaatt gaacatacga tcttggataa tattattcga
     8701 atcataaaaa tcaacagaaa aaacggcctt tttagacata gaaggttgta atttaccctt
     8761 ggtcaaaatt ttgtttggac tcgtatcata tttatctccg ttttctgtca tcttcgcgac
     8821 attagctgta acatcaaatt gaagcgggat atcgtccacg ttgaattcgc ctcgcgtaat
     8881 aatagcaata atatctgcca atagagtatt tgtatcccac gaccccatcc caaaacgcaa
     8941 ggtatggctt cctgctgata ataaactacc gctagtaacc ttgatggggg caccggcaat
     9001 atacaagtag gcgttgttcc agtcaattgc ttttaataca tcactgcttg atacatgttc
     9061 cggcaaattg atgatcgcca caaatgcaaa tccttgtgca gtgatattca ccaaatcaag
     9121 tacatttgtt agtttcacat ggctttccca atgaaaacgg accgtgccac ctttccaagg
     9181 atcatttgaa ccatcgttca aataagtagc agtcaattta ggcaattggt tagccccagt
     9241 tagttggaca agcttcccgc
//
LOCUS       DACCQI010000107         9023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCQI010000107 DACCQI010000000
VERSION     DACCQI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.111"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(310..807)
                     /locus_tag="IVO76_002258"
     CDS             complement(310..807)
                     /locus_tag="IVO76_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7430197.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(847..1656)
                     /locus_tag="IVO76_002259"
     CDS             complement(847..1656)
                     /locus_tag="IVO76_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7430198.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            1990..2847
                     /locus_tag="IVO76_002260"
     CDS             1990..2847
                     /locus_tag="IVO76_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7430199.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2840..3742
                     /locus_tag="IVO76_002261"
     CDS             2840..3742
                     /locus_tag="IVO76_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7430200.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3775..4125
                     /locus_tag="IVO76_002262"
     CDS             3775..4125
                     /locus_tag="IVO76_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7430201.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4159..5097
                     /locus_tag="IVO76_002263"
     CDS             4159..5097
                     /locus_tag="IVO76_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7430202.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5175..6068
                     /locus_tag="IVO76_002264"
     CDS             5175..6068
                     /locus_tag="IVO76_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7430203.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6106..6669
                     /gene="amaP"
                     /locus_tag="IVO76_002265"
     CDS             6106..6669
                     /gene="amaP"
                     /locus_tag="IVO76_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7430204.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6683..6871
                     /locus_tag="IVO76_002266"
     CDS             6683..6871
                     /locus_tag="IVO76_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7430205.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6884..7786
                     /locus_tag="IVO76_002267"
     CDS             6884..7786
                     /locus_tag="IVO76_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7430206.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7818..8060
                     /locus_tag="IVO76_002268"
     CDS             7818..8060
                     /locus_tag="IVO76_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7430207.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8316..8687)
                     /locus_tag="IVO76_002269"
     CDS             complement(8316..8687)
                     /locus_tag="IVO76_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430208.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3160 a         1552 c         1740 g         2571 t
ORIGIN      
        1 ttggctgttc ttattactgc agatgcaagc ttttgaatac cgtttaagga agaggttgtg
       61 aaatcagcgt tttgacctga gtattagctt aacaaacgga aaaatagttc ctcatatttt
      121 gccgtttgtt aagctaatac cgagggtcag ctgattgaac accatttaag aaagaggtcg
      181 tgagcctgcc gtttcactct gtgtcataaa aaagccaatc tactttgata gaaaaagcac
      241 gtaggactaa aacagttctg cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga
      301 tagatatttt tatgattcga cgatagagac gttgaaatct ttcaacagct ctaaagcgga
      361 ttctccgtgt tttctactgg agactaacat tggatcaaca aaaaagtctg cttcaggaaa
      421 agcactctgc aggcctatca gattagagag tacacacaag tttgtttcaa tcccgactac
      481 ttcgatctct ttttgttctt ctttgctttc aaataaagtt tcctggattt cagctaaagt
      541 ttcaggtgga atcgtaaagt aatacttttt aatcactcgt tcattttctt gtggagaaag
      601 aagagggatc aattgcaaat cttctcgttc ttcttctttg tccttcagtt caactggtat
      661 atcccgtgtg aagagaatat attcattatt ttggcgtgct ttagctaatc tttgagcaat
      721 acgttctgcc aattgatctg actctggaat ataaaattcg ctgtttggat caagaatatg
      781 attttgcata tcaataataa ctaacatttt ctttcctcct acattcatat cgtcatttta
      841 ttctttttaa tagaagtttt ttaaaatata agcaacacca gcttcttcat tcgtcagtgt
      901 tacttggttt gcaatttctt ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc
      961 gctgtaattc aacatagcaa tgtcgttttc ttgatcgcca aaagccatga tgtcactcga
     1021 ttcgattcca aggtaatccg ccagaaaaac aagtgaatga gctttagtga tacctgtagg
     1081 cgtgatttct aagaaatgcg gatcagagaa tactgattgg ataccatctg cttgcagatc
     1141 tctttgtagc ttctgtagtt tttcttcact cttatctgtt aatgctaatt taagatagct
     1201 aagagaagga tcttccaaca tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa
     1261 atgttcataa taagggtcat ctttgactag atttgattca tcgaatgtca gctgatcatc
     1321 ttgggtttct cggctagcaa tgatggatat tttcgcttct tgagcataga agaaggcttt
     1381 tctcagtgta tcgctatcta tttttgaacg tctgagaact ttcgccattt ttccatcctt
     1441 catttcagca atcagtgaac cattggaaga aatcaaatag ccaccgattc ctaattcgga
     1501 aaaaatatct ttagcagaaa tgctgttcct tccggtagcg acggttacga ttccgccatt
     1561 ttcatcaaat ttccgaagtg cttggatatt ttcttctggt atctttccat ttcgatcaag
     1621 caaggtaccg tctaaatcaa tcgctgctag tttcatttta tcttcctact ttctttttac
     1681 atatctattt tcctgttacc ttaagattaa atgagaattt aaaaaaatga aactaacttg
     1741 ctttgtcatt atgaattttt tcaaaaaaca ttcttaagaa aaaggtggct atatatagta
     1801 gtttttggtc aatacctttg aataaaaagc catgaaaaac gaatagttag cgcttaccga
     1861 tgcgttttgt gataaaaaaa ttttaaaata ttcaatgaaa acaaagcata tattttgctt
     1921 taaaaccaaa tgaggaatat tctagatgta gaacaggtta gaaaaagatt agaaaaagga
     1981 gtgattttga tggagaagag atacggcgta gttcctgtgg taactgcttc agacgaaaat
     2041 tatgctcctt acttgagcgt gatgatcgca actgcattag aaaattgcaa taaaacaaga
     2101 cgcataaaat tttatgtgat tgacgatggt ctatcagaat atagtaaaga aggattggaa
     2161 gaaacggtaa ataaatattc aagtaatgca agtatccaat tcctgacagt ggaaaaagat
     2221 atctacgaag acttcctagt cagtgaccat atcacaacta ccgcttactt aagaatttct
     2281 ttaccaaact tattagctaa agaggattat aagaaagtcc tttacttaga ttcagatgtg
     2341 ttagtactag acgatatcgt aaaactatat gatgagccgt taaatggaaa aacaatcggc
     2401 gctattatcg atccaggcca agtgaaagct ttggaaagat taggaatcga ttccgatgac
     2461 ttgtacttca attcaggagt gatggtgatt gacattgatc aatggaataa aaaagaaatt
     2521 acagaaaaaa ctatccatta cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa
     2581 gatgctttga atgctgtact ttatgaagat tgggaacaac ttcatccaaa atggaacatg
     2641 cagacttcgc tgatttttga acgtcatcca gcaccaaatg aaaaatatga acgtcagtat
     2701 aaagaaggaa atgaaaaacc ttcgatcgtt cactttacag gacatgataa accttggaat
     2761 acgttgaaag atcatccgta taccaattta tacttaaaaa aattagcaca cagtactcta
     2821 acgaaagtag gcgagatcaa tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac
     2881 aaaattcgta ccacatttag ctgcattatt tgtctcagta ttagataatt gcaacccgtc
     2941 taaatttgtt cggttctatg tgattgacga tgatatcgat tttgaaagta aacagctttt
     3001 acgcttctca gtgaaaaacg cacgaatgaa ctcagatgtg gaattcttga aaatcaataa
     3061 agaattcttt acaaatgtgg taatcagcga tcgtattcca gaaacagctt attatcgtat
     3121 tgcgattccg gaattgttcc gcggcacaga agttgaacgt atcttatata tggattgcga
     3181 catgatcgct ttacaagata tctctaaatt atggcgtctt gacttcggtg attcaatcgt
     3241 agcagctgtt gaagacgctg ggttccatca acgactggaa aaaatgaaaa tttcagctaa
     3301 atcaatgcgt tatttcaact caggtttgat gctgatcaat gtgaaaaaat ggttagatga
     3361 aaatattacg caaaaagtat tagatttcat cgaacataat ccagaaaaat tacgattcca
     3421 tgaccaagat gcattaaatg ctatcttaca tgaccgttgg ttaccacttc atccgcgttg
     3481 gaatgcacaa ggctatatca tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg
     3541 tgaatacgaa gaaactcgca acaatcctta cattatccat ttttcaggac atgtgaaacc
     3601 gtggagcaag gattttgaag ggccaacgaa gaaatattac gaaaaatatg cagggatgac
     3661 ggctttccgt tgtgtagcta aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa
     3721 aacagatgta gttgttaaat aatagaagac aacaactgaa gagggggagt aggaatggaa
     3781 attacaatgt ctaaaaatgc agtagagacg ttaattgaaa aggttgggga aaatacaaaa
     3841 atcgcactcg cgcttatcaa tgattccgat ccatttctaa gggacaaagg tgcttttgca
     3901 aaaggcagct tttttcaaat cattcctttt gtttcggaat ttggagaata tgcgactaaa
     3961 atcgagcatc cattattgga catctataca tctaaattag agcaaaatta tttcggtaaa
     4021 cgattgaaca tggacttcaa caaacagctt gacagctttt cattagaaaa tgaaatcgca
     4081 gtactcgatt ataatatcaa attaaagaac tgcttcttca gctaatagtt tcgagaaagt
     4141 ccaaaaagga ggtatacatt gagatactac tttgtggatg aagaaaagaa tgtaatagaa
     4201 acagatgaaa aaaaatataa ttggctcgta cttgatagtg caaagcccga agagattgag
     4261 caagcaatcg aagaattcga gcttcctgat gatattttcg taggaaccga atatccggaa
     4321 gaagtttctc ggatggagcg cctttatgag acgaaactag agcatccatt ttctttggta
     4381 gtgattaatc tagatcagtc agataatcgt atcgaaaaaa aattaacgcc aatatctttt
     4441 gtactttccg ataatctctt gattacttgt atcgatagaa aaaccgaatt tgtggatcgg
     4501 ctgctgacaa agcatggaaa tcatttaaac tcgtttgaaa agatcatcac gtatacatta
     4561 ttagatattt acacccatta cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct
     4621 ttggataaag cagctcgtaa aacaacggaa aatgaagaac tgtataaaca ggcagatctt
     4681 gaaagagaca tcgtatatat cgatcatacc ttaagagatc aacgggaaac aatggatcaa
     4741 ttatggaatt ctgaaacttt catcaaacgt ttagacgatg aacagctact ctatgatgtc
     4801 catttgagac agcgacaaac agaaaaaatg atcgagatct atcgagattt attagagagt
     4861 attggtgatc tattcaacgg gatgatggac aataatctaa atcatatcat gaaatatcta
     4921 gattcagctg cattaatcat ttccgttccg gcgttgttcg cagggatttg gggaatgaat
     4981 acaggaggat taccgggtaa aagttctgct ctaggcttta tactcgtagt ctttggctct
     5041 ttgatagcag cagtagtttt aggttaccat ttataccgga aagattatac aaattaaaga
     5101 atctatttaa aacaggtatc aaacaaagaa atttgttttg gcaatacaat ttaaaaacta
     5161 ggaggaatac agcaatgaca gaaccaaaat taatcgaccc gcgaaacttg taccatacgg
     5221 agaaatttcc taaacaagat caggatacac cagctttaca gcagaatatg attcctgttc
     5281 ctgattgtgg agaagaatcc tatgaaggca ataatcaact agaaaaccgc cgtgtactaa
     5341 tcactggtgg agattcaggg atcggtcgtg cagcagcgat tgcatttgct agagaagggg
     5401 cagatatcgc tcttcaattc ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat
     5461 acgtgaaaga tgcaggacgt aagattgtct tactaccttt tgatcttcgt gacgaacaag
     5521 cacctaaaga aatcatagat aaagccgtga gtgaacttgg cgggctagat acgatggtac
     5581 taaacgcagg acagcaaatt tcttgtcagt ctattgctga gttaccactt gagcaagtca
     5641 aagacacatt tatgataaac atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc
     5701 atatcccagc aggaggagca atcgtaacaa caacttctgt tcaagcattc aatccaagcg
     5761 aacatttatt agattacgca gcaacaaaag cttcaatcgc taactttacg atcggtttag
     5821 caaaacagct tgcacctaaa ggaatccgtg taaatggtgt agcacctggt ccaatctgga
     5881 caccattaca actagacgaa ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa
     5941 gtttactaga acgtgcaggt cagccaggag aattagcacc agtttatgtt ttcttagcat
     6001 cgaataaagc aagttatgtt acagcccaaa tttacggagt aactggtgga gaagcgatca
     6061 acttataaga agttgatcgt ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta
     6121 aaaacgatcg gagtgatcgg aacattactt ttgctatctg tgcttttgtt tgcgatacta
     6181 ttaagtgcaa cctatccatt gccttttcag ctagagaagt ttcgattctt ttccatcact
     6241 aattcatacg tgcaacaata tgtattttgg cttgccgttg catttgctat tttgacaatt
     6301 atcacgttac ttgttttcct gttttatcca aaaacaatcg gaaactttct actgaaacat
     6361 ggtgatgggg agttgacgtt ggataaaaaa gcaattgaag gaatggttcg ttctcacttg
     6421 catgaagatg aattcatcca ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc
     6481 gccgtcaatg taaaagggga gttgaaacga acttcagcac tcattggtaa aacaggaaca
     6541 ttgatgaaag aaatcgaaaa tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa
     6601 gtatcggtga aatacacagg ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga
     6661 gtagaatagg gggggaccaa aaatgaaaga actattttcg acatacaagc tccctatcat
     6721 ctttggggta cttggattga tattagccgt tttatggatc accgtgggct ttttcaagac
     6781 cttattgatc ttgatcatgg cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac
     6841 gaaaatctta gatcactatt ttaaatctta ggaggaaaca tccatgaata acaataacaa
     6901 aaataataac aataaaatga acaaccaaaa accagtgtct gcacatccag acccaacgac
     6961 accaaaccaa tcaaatggtg tgaacgatga aaagaacaaa acagcaccag tatctgcaca
     7021 tccagatcca gtagtcccta acgttcaaca aggagtagaa gagaagaaac atgatgcaga
     7081 aaagcatgca cctcatcatc caggaactcc agaatctaaa gttccagaac aacatgatgc
     7141 aatcgatcct tctaaacctg tggaagagca aaaacatcct tctgaccatg cgaaagcaca
     7201 agaaaaactt agttctgacc acacaaacgg acaaggaaaa cttggttctg atcatacaaa
     7261 taaccaagga aagcatgatt ctgaacagga agattcaatg cgcggtgagc tgaattatga
     7321 agataaagtt gtgcaaaaaa tcatcggaat cgcaattgaa caagtagacg gtttattatc
     7381 tgcaaacggt ggtttcttct ctaatgtggc aggaaaactt gtgaatacag ataatgtaac
     7441 ggctgggatc gaaacagaag tcggtaaaaa acaagtagca gttgatatgg atgtcattgt
     7501 ggaatatggc aaagatatcg aaaaaatctt tgaagaaatg caagaagtaa tcggcaaaga
     7561 agtaaaaaat atgacacatt tagaagtgat cgaagtcaac gctaacgtag tggacatcaa
     7621 aacaaaagaa gaatttgaaa aagatcaaga aacggtgcaa gacaaagtaa ctgacgcagc
     7681 gaaaaaaacc ggtgaatttg cttcgaatca aacagataaa gtaaaatcag cagctggaag
     7741 cggcgcacaa aaagtaaaag aaaatacaga accacgggtt cagtaataag gaagtaaaac
     7801 attaggagga acgaaacatg ggatttattt ggtcacttat cgtcggagga attatcggag
     7861 caattgcagg agcaatcacg aataaaggtt cttcgatggg gattattgca aatgtcgtag
     7921 caggtttagt tggatcagca cttggtcaag cattattagg ttcttgggga ccaagtattg
     7981 caggaatggc aatcgtgcca tcattgattg gtgccatcat tttagtacta atcgtatcat
     8041 tctttgctcg aaaactgtaa aaagactttt aaaaagaggt cgtgaaaaag ctgttcttca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttgggcat
     8161 attatcgaag atgcaagctt ttgaacactg tttatacgga ataagaggct gtgacaagac
     8221 ttttgtcaca gcctcttatt tatgcgggca aattcccaat tagattttta aaagtttttc
     8281 cttaaatgtg tctttattga tacagagaag cttctttaat aaaacaatgc ctccctaaat
     8341 tttcttgaga taggaaaggt gaacatacaa aataatacga ccactttgaa aacccagatg
     8401 atccacaaat ggatcgtatg atactgatga agtaaaaagc aatggatcag aaactcaaag
     8461 agattgaaaa agacaaggaa gaaataatat ctctttttta ctcttgtctg ttttagccaa
     8521 taatagctcg taaagtaatc tagacaaaca aatagaagta aacaaagttt cgtaagagca
     8581 gcaatcgtat catgttgctc tgctagacga ttgacgagca ggaaaaatgt tggtaatgga
     8641 atatagatca cgagtaatga aagaaaaaag taaacaataa atggcatact attcctccta
     8701 attgtgtgag ttcgccctca ttttaaaata acgatatttg tttgtgaagc tttttcgtgt
     8761 cgtaacaaat gttatctaat gtatttttaa ataaataatc attattatca aaataatgtt
     8821 aaaaataaaa aagaattgtc catcactgtt attcaaatca ttttatcaaa actacatagt
     8881 aaatgttaaa aagcaacatg acataatata tagaaggctt tacagtatat atgggagctt
     8941 actaaaaaag aatggagtaa gagttaacac aatgggccat tagctgaagt tttaacagaa
     9001 ttaggcagaa gtcgcccatc ctc
//
LOCUS       DACCQI010000108         8960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCQI010000108 DACCQI010000000
VERSION     DACCQI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.112"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..361)
                     /locus_tag="IVO76_002270"
     CDS             complement(<1..361)
                     /locus_tag="IVO76_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7430209.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
     gene            complement(713..1072)
                     /gene="rplT"
                     /locus_tag="IVO76_002271"
     CDS             complement(713..1072)
                     /gene="rplT"
                     /locus_tag="IVO76_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7430210.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="IVO76_002272"
     CDS             complement(1125..1325)
                     /gene="rpmI"
                     /locus_tag="IVO76_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7430211.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1397..1921)
                     /gene="infC"
                     /locus_tag="IVO76_002273"
     CDS             complement(1397..1921)
                     /gene="infC"
                     /locus_tag="IVO76_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7430212.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2241..2873
                     /locus_tag="IVO76_002274"
     CDS             2241..2873
                     /locus_tag="IVO76_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7430213.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2875..3780)
                     /locus_tag="IVO76_002275"
     CDS             complement(2875..3780)
                     /locus_tag="IVO76_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7430214.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3865..4518)
                     /locus_tag="IVO76_002276"
     CDS             complement(3865..4518)
                     /locus_tag="IVO76_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7430215.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4840..5784
                     /gene="mvk"
                     /locus_tag="IVO76_002277"
     CDS             4840..5784
                     /gene="mvk"
                     /locus_tag="IVO76_002277"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7430216.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5777..6754
                     /gene="mvaD"
                     /locus_tag="IVO76_002278"
     CDS             5777..6754
                     /gene="mvaD"
                     /locus_tag="IVO76_002278"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7430217.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6767..7852
                     /locus_tag="IVO76_002279"
     CDS             6767..7852
                     /locus_tag="IVO76_002279"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7430218.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7849..8913
                     /locus_tag="IVO76_002280"
     CDS             7849..8913
                     /locus_tag="IVO76_002280"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7430219.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
BASE COUNT         2695 a         1859 c         1736 g         2670 t
ORIGIN      
        1 aaatacctaa tgacatcaag atcggcaaca caataactgc aatagaaatc actggtccga
       61 ttcctgtcat cgacatgaat ataacagcgg ttacaataca tagtaagctc atcgtgattc
      121 ttggcttgtc ccctccaagt tcaactacct ttcgaatcag ggtagctgcg attccgctat
      181 ctactagaac acgcccgaaa aaggcaccaa agaagacatt taccaagatc gatttggcgt
      241 actcagctgg ccccgtcgta tagatatgcg tcaacacatc aattactcct tgcccttcca
      301 tcgccgagtt tggtgaaaac gtgtttccga ctagcgctaa tcccatccat acagtagcca
      361 tgatgacaaa agccgaccat caagttgtag ccttttacac agtaccagac catgaaaaag
      421 aatgagataa ctaacaaaat accaataata atatcactca tgttattcct ccgtccattt
      481 tgtttttctg ataagcaaac taatatatca gtcaaactca ttctaactta tgtagttagc
      541 gctttcatta aaggatgacc ataaatgttc agttttcaat taaaaaacct taattatgtt
      601 attttagact tatacgttat tgtcctgttt catttgatgg aggattgtaa ataaaaaaag
      661 aggaaagaac ataacttttc gttatcttct ttcctcttta taattgttta gcttatttag
      721 ccaaagcttc ttttgcttgg tcagccaatg cagtaaatgc tgctgcatcg ttaacagcca
      781 agtcagctaa cattttacgg ttgatgtcga tttcagcaac tttcaaaccg tgcattaatt
      841 ttgagtagct caagccattc atacgagctg ctgcgttgat acgagcaatc cacaatttac
      901 ggaagtcgcg tttcttttga cgacgatcgc gatatgcgta gttgtatgaa ttcattactt
      961 gttcttttgc tgttttaaat aaagtgtgtt ttgaaccgta gtaacctttt gctaatttta
     1021 gcactctttt acgacgttta cgagttactg ttccaccttt aacacgtgcc atgtataatt
     1081 cctcctaata aaaataaatc ttcagattct gttatttgta cttcttattt catttttgct
     1141 agttgttggc gaatacgttt gaagtcgcca ctagaaacca tgcttgcttt acgcaattga
     1201 cggcgttgtt tttttgtttt gccgtggaaa cggtgacttg taaacgcgcg gaagcgtttc
     1261 aatccgcctt taccagtacg tttgaaacgt tttgctgatc cgcggtgtgt tttttgtttt
     1321 ggcatgacta tatttcctcc tcaaaatctt tcgttatccg actatcggct gtccaacagc
     1381 cagttagtca ttcaaattac ttactgtcgt ttttcggtgc cagcgtcaag aacatgctgc
     1441 gtccgtccat tttcgctttt tgttccactg ttgcaatatc cgcagtttct tcagctaagc
     1501 gattcaagac tttttgacca atttctttat gggtaatggc acggcctttg aaacggatag
     1561 aagctttcac tttatcgccc ttttccaaga acttacgtgc attacgaagt tttgtattga
     1621 agtcattcaa gtcgatcgta ggacttaagc gaacttcttt tacattgatc actttttgtt
     1681 ttttacgcgc ttcgcggtct ttcttttgtt gttcgaaacg gtatttaccg tagtccatga
     1741 ttcgcgcaac tggtggtttc gcaccagggg caactaacac aagatccaag tttgcttgtt
     1801 ctgcaatatt caatgcttct gcttttgtct taactcctaa ttgctcacca tctgaaccga
     1861 tcaatcgtaa ctctcgtgca cgaatgccgt cgttaaccat catatccttt gctatggtca
     1921 ttcacctcca aatattatga gagaaagaaa agcttaccga ataaacggca ataaaaaaac
     1981 gagttctctt acagaacccg cgcgtacttc acctaataga accagtgaaa actatctagc
     2041 ccagtgacat tatcaactaa ggcgagaagc gggtgcttct gcttttattt ctcaaccatt
     2101 atagtatagc aaaaactaat ctttttgtca atgtttttca tacagtttgt cttccttttc
     2161 tgaaaatgat tagattagca ggctttctag cagtgattcc cgcttctttg ctatactaaa
     2221 gaaaaacgag gtgaacccac atgaaaaaaa tcgaacctat tccaccttct cttcctcttc
     2281 ttacaaaggg agagttttat ttagaagatg aggagatctt ctctggtctt ctgatcaacg
     2341 gaggtaacca aagctatctc gatgccaaaa atatggtatt aagagagagt catatccaaa
     2401 aacttacgat gcaaaaaaca ggtttacgtc attttgaatg cagtaatgtc atttttgaaa
     2461 aatgtgattt ttccaatctg gagtggattg gtggcagttt ccaccaagtc gtgtttcatc
     2521 aatgtaaatt aacaggtacg aattttgccg aaagttatct tcgagactgt actttcactg
     2581 attgtattgc ttcgatggct tcctttagta gtacaaactt gaaagctgtc tccttcgatc
     2641 attgtcaatt agaagacagc gaattttatg aggtcacttg gaaaaacctt tttttgtctg
     2701 acaatcagtt aaccggttcg aactggtttc gaacttctct gaagggatta gactttacga
     2761 ctaatttctt cacaacaatc gctttatctc aagattcttt ggcgggacta atcgtcaatc
     2821 aagagcaagc acttgtgatc gcccaagccc ttggtttgca gatcaaagac tagatcaacg
     2881 aatccgccgc cacagtgcaa taagcatcca aatagataag accaaactga tgatcaaggc
     2941 gatttcccat ccgaaaacgg aatgggtaaa gggtaactgg agattcattc cgaagaatcc
     3001 tgtcacaata gttggaacgg tcaagatcag cgaccagacc gtcaaaaatt tcatcgtgtc
     3061 attcaagttg ttattcagca aattattata agtgccagag agctggtcta aaatctgcga
     3121 agctaagttc gtcatttcaa ctgcttgtcg tgcttcgatc agtgcatcgt ccaactcttc
     3181 tttttccttt tctgacaact tgcgataaat agccagtgcc ttgatttgtt ctaaaagtac
     3241 tacattttgt tttgttcctg taactaagta aaccaaaccg atctctaaat ctgaaagttg
     3301 cagcagattc ttgttcgttg ttttttctct taatttcaga ttaagccgct gacgttcact
     3361 attcacttcc tcgattaaag gaaaaaagga atcagaaatc aaaaacaatg tacgaaacaa
     3421 taaactgtat acactcatgt ctggcatccg ttccaaaagc gatcgaatta gtcgaatcac
     3481 gtactcggta ttcggcgtgg taaatgtaaa caaaccgtct tcttttaaaa taaaagcgat
     3541 aggactagtt tcataatgat tatcgacttt ttgctggtgc ggaacatttg atacaagcag
     3601 taacgcttca tcaaaagaat cgtactctac gcgcgcccgt tcatttttat ctagagaata
     3661 agtcagcatc tcgtcactaa tgccatattc ttcttttaag tttttaacag ctagcacatc
     3721 gttttgctgt acattgatcc aaaaattacg gtcttgatta aaatagtgtt tttcccgcat
     3781 actctatcct cctgacattt gtttccagta taaagaaaaa agtcgctcaa ataaaggttt
     3841 accctttatt tgagcaactg taaatcaaat ggatactttg gtttggcgaa tctctgctaa
     3901 tttttcttcg atcattttca agacttcttc ttttgcttga ggatcttcca caaaattata
     3961 gcggtctcca tcgatttgga tttttggact ttctttgtat tcttcatacc attgatcata
     4021 acgtctgttc aattctttgt aataatcgta tagacttggg tcaaaggaca attgttcata
     4081 cgaacgacca cgtttttcga tacgctctaa catcgtatca aaagatacac gaatatgaac
     4141 taataaatcc ggatgttttt tatgggcagc atagggaagt tcttccatca tgttttccag
     4201 cagttcatca tacacacaaa cttctgtttc tgttgctctt cctagatctg catttagatg
     4261 gaaaagcagt gaatcttcat aaatcgaacg gtccaataca ttattgtcat ctttcattgc
     4321 ttgtttgatg cttgcaaaac gtttattcaa aaaataaatt tgcagcaaaa aagcatactg
     4381 ttcagggttt gcataaaata agggcaagac ttcattatca tcaatcgatt cataaaatgc
     4441 ctgactatca aagtgttcag ccatcatttc tgttaaactt gattttccgg caccaattgt
     4501 acctgctaaa acgatcacgc tcatttacct ccagctcact tcatcactaa atattgtgtt
     4561 taactccaac cttttttaaa tataccccca atatctagta gtgtcaattg tattttttct
     4621 agtcaaagaa gaaaaaaagt caaaaaattc ttaagaaaaa agtgtttttt ctttagaaaa
     4681 cgtattctta gttttacttc tgagatggtt ttagtaaaat aaaggaggaa tgttttttga
     4741 gacccttagt ggacaaaaaa attcgtgaag cagcgcaact cgttttgggc gctttatttt
     4801 ttgcttaaaa atcactttta cgtgagaagg agttagatta tggcaaacta tggccaagga
     4861 gaatcaagcg gaaagatcat attgatgggc gagcacgcgg ttgtttatgg agaaccagcg
     4921 attgcctttc ctttctatgc aacaaaagtc accgcattcc ttgaagagct ggatgcaatg
     4981 gacgatcaac tggtttcttc ctactattca ggaaatttag ccgaagctcc tcatgcatta
     5041 aaaaatatca aaaaattatt cattcactta aaaaaacagc atgacatcca aaaaaacttg
     5101 caactgacca ttgaaagcac gattcctgct gaacgtggaa tgggatcaag cgctgcagtc
     5161 gccacagcag tcactcgtgc tttttatgat tacttagcat ttcctttgtc tcgtgaaata
     5221 ctattagaaa atgtccagct ttcggaaaaa atcgcccacg gtaatcctag tggaatcgat
     5281 gcagccgcta ctagcagctt gcagccgatt tattttacaa aagggcatcc ttttgactac
     5341 ttttctttga acatcgatgc ttttttgatt gtcgctgata caggaatcaa aggacaaaca
     5401 agagaagccg tcaaagatgt cgctcacctc tttgaaaccc agcctcatga aactggacaa
     5461 atgatccaaa aattaggata cttgacgaag caagcaaaac aagcaatcat tgagaattca
     5521 ccagaaacgt tggcacagac aatggatgaa tcacagtcac ttctggaaaa gctgacaatc
     5581 agcaatgatt ttcttaatct attgatccaa acagcaaaag ataccggggc attgggtgcc
     5641 aaattaactg gcggtggacg cggtggctgt atgattgcat tagcacaaac aaaaacaaaa
     5701 gcccaagaaa tcagtcaagc acttgaagat gcgggtgctg ctgaaacttg gatacaagga
     5761 ttaggagtac atacctatgt ttaaaggcaa agcacgcgca tataccaaca ttgctctaat
     5821 caaatattgg ggtaagaaaa atgaagaact catccttcca atgaacaaca gcctttcgtt
     5881 aacattggat gcattttata cagagacaga agtcatcttt tctgatagct atatggtaga
     5941 tgaattttat ctagatggca ccttacaaga cgaaaaagca acaaaaaaag tcagtcagtt
     6001 tcttgacctt tttcgtaaag aagccggcct ctcactaaaa gcttcagtaa tcagtcagaa
     6061 tttcgttcct accgcagctg gattagcctc ctctgccagc gggctagctg ctttagcagg
     6121 agcttgcaat actgctctta agcttggatt agacgatctc tctctttcaa gatttgctcg
     6181 acgcgggtct ggttcagctt gccgaagtat tttcggtggt ttcgttgaat gggaaaaagg
     6241 ccatgacgac ttaagttctt acgctaagcc agtcccttcc gattctttcg aagacgattt
     6301 agcaatggtt ttcgttttga tcaacgacca gaaaaaagaa gtgtccagca gaaatgggat
     6361 gcgtcgaaca gtcgaaacat ccagttttta tcaaggctgg ttagattccg ttgaagggga
     6421 tctatatcaa ttgaaacaag caatcaaaac aaaagatttc caacttctcg gagaaacgat
     6481 ggaaagaaac ggactaaaaa tgcatggaac gactttagct gcccagcctc cattcactta
     6541 ctggtctcct gacagcttaa aagcgatgga tgcggtgcgg caattgagaa aaaaaggcat
     6601 tccatgctac tttacgatgg atgctggccc taatgtcaaa gttttagtgg aaaacagcca
     6661 tttatctgag gtacaagaaa catttaccaa attatttagc aaagaacaag taattactgc
     6721 ccatgctggt ccaggaattg cgattattga ataaagggag atttttatga ttgaagtatc
     6781 tgcaccaggc aaactctata ttgccggaga atatgcagtt gttgaaacag gacatccagc
     6841 agttatcgct gcagtcgatc aattcgtaac agtcactgta gaatccgcac gaaaagtcgg
     6901 aagtatccaa tctgcacaat atagtgggat gcctgtacgt tggacaagac gcaacggaga
     6961 attagtttta gatattcggg aaaatccttt tcattatatc cttgctgcta ttcgtttgac
     7021 tgaaaagtat gcacaagaaa aaaacattct tttatccttt tatgatctga aagtaacaag
     7081 tgagttagac agttcaaatg gccggaaata tggattgggt tctagcggtg ccgtgaccgt
     7141 tgcaacggtc aaagccttga atgtttttta cgcgttgaat ttatctcaat tggagatttt
     7201 caagattgca gcactagcca atttagcagt tcaagataat ggttcctgcg gcgacatcgc
     7261 tgctagctgt tatggtggct ggatcgcttt ctcaaccttc gaccatcctt ggctccaaga
     7321 acaagaaact cagcattcta tcagtgagtt acttgccctg gattggccag gtctatccat
     7381 tgagccattg attgctcctg aagatttacg tttattgatt ggttggacgg gtagccctgc
     7441 ctctacttct gatttggtcg atcaagttca ccgttcgaga gaagacaaaa tggtggctta
     7501 tcagcttttc ttaaaaaaca gtacagaatg tgtcaacgaa atgatcaaag ggtttaaaga
     7561 aaataatgta acgttgatcc aacagatgat tcgaaaaaac cgacaattac tgcatgattt
     7621 atctgcaatc actggggtcg tcatcgaaac gcctgctttg aacaaattgt gtaatttagc
     7681 tgaacagtat gaaggagccg caaaatcttc tggtgcaggt ggtggcgatt gcggaatcgt
     7741 aattgttgac cagaaatctg gcatccttcc tttaatgagt gcatgggaaa aagcagaaat
     7801 cactccactg ccgttacatg tctatagcga tcaaagaaag gaaaaccgat gaatcgaaaa
     7861 gacgaacatg tttcactagc taaagccttt catgataaac aaaaaaacga atttgacttc
     7921 gtacggatca tccataaccc tttgccgcaa atagcagttt ctgatgtcga tctaagtaca
     7981 caggcagttg ggtttacact aagcagccca ttttatatca atgcgatgac tggcggaagc
     8041 gaaaaaacaa aaaaaatcaa ccaagatctt gcaatcgtcg caagagaagc agatttgatg
     8101 attgccactg gttcagtcag cgctgccttg aaagaccctt ctttagccga tacttatacg
     8161 attatgcgac aagaatatcc tcacgggaaa atcattgcca acatcggtgc agggacttct
     8221 gtcgaaagag cccaagaagc tatccgattg tttcacgcag atgccttaca gattcatttg
     8281 aatgcacctc aagagttggt tatgcctgag ggagaccgtg attttaccaa ttggaaagta
     8341 cttattcaag agactcaaac agccattgac gtccccctca tcgtaaaaga agtcggtttt
     8401 ggcatgacaa gagaaacgct caatgatcta gctgctttag gagttcatac agttgatatc
     8461 agcggtcgaa gcggaacaag cttcacccag atcgaaaacg cccgtcgctc caagcgggaa
     8521 ctgagttatt tagctgactg ggggcaatcg acagtctctt cattgcttga agcaaatgaa
     8581 gcagacactt ctatggaaat cctagcctcc ggaggtatcc gcaatgccta tgacatattt
     8641 aaagcccttt gtctcggcgc aaatgcagtg ggtacttcag gaaccgtact gacgcatctg
     8701 atgaatcacg gcgtagaaga aactattata ctgatgaagc aatggcaaga agagcttcgt
     8761 ctgctttata ctatggtcgg cgcaacgaat accgcggccc tacaccaaca atcactgatc
     8821 ttttccggac cagtgaagga ttggtgcgaa gcaagaggga tcgacttagt gaagtatgga
     8881 agacgcacag aaaagagcgt gggacaaaag taaagaacac ttttatccca cgctctaaac
     8941 acgtataaac ggcggaagca
//
LOCUS       DACCQI010000109         8894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCQI010000109 DACCQI010000000
VERSION     DACCQI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.113"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(221..1177)
                     /locus_tag="IVO76_002281"
     CDS             complement(221..1177)
                     /locus_tag="IVO76_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7430220.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IVO76_002282"
     CDS             1281..1883
                     /locus_tag="IVO76_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7430221.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IVO76_002283"
     CDS             complement(1971..3470)
                     /locus_tag="IVO76_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7430222.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IVO76_002284"
     CDS             complement(3486..4010)
                     /locus_tag="IVO76_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430223.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            complement(4096..4281)
                     /locus_tag="IVO76_002285"
     CDS             complement(4096..4281)
                     /locus_tag="IVO76_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430224.1"
                     /translation="MAETIKETNVEKQTTRMIVFWSFNGFFLKKGNIGYLERIYCENF
                     NMKTKFNKLSTINTTFL"
     gene            4307..5056
                     /locus_tag="IVO76_002286"
     CDS             4307..5056
                     /locus_tag="IVO76_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7430225.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5047..5832
                     /locus_tag="IVO76_002287"
     CDS             5047..5832
                     /locus_tag="IVO76_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7430226.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5801..6517
                     /locus_tag="IVO76_002288"
     CDS             5801..6517
                     /locus_tag="IVO76_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7430227.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(6619..7638)
                     /locus_tag="IVO76_002289"
     CDS             complement(6619..7638)
                     /locus_tag="IVO76_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7430228.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7657..8715)
                     /locus_tag="IVO76_002290"
     CDS             complement(7657..8715)
                     /locus_tag="IVO76_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7430229.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         2683 a         1796 c         1590 g         2825 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttga
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttccg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaattttgt
     4141 tttcatgtta aaattttcac aataaattct ttcgaggtag cctatgtttc ctttttttaa
     4201 gaaaaaacca ttaaagctcc agaacacgat catccttgtc gtttgtttct ccacattcgt
     4261 ttctttgatt gtttctgcca tattgatcag gaattttatt ctttcgatga accgatctag
     4321 tgtgtcagat aaaatctcta gcattgctaa aatcgttgcc aatgataaac tcatccaaaa
     4381 aaagctgcag actggtgacc cgacacatgc gattcaagga ataacaaatg aaatcatgat
     4441 tgcatcgaat ttagatttta tcgttgtcat ggaccctgaa ggcattcgat attctcacgc
     4501 agacccaagt tacatcggtc ttcctttctc aagtcatgaa gatgtgaaaa aagtgattgg
     4561 aggacaagaa tttttctctg aaaaagaagg aatgttaggg ttcggcttcc gctatttcgt
     4621 ccctatttat tatgatcaag agattattgg tgcagtttgt gttggcttaa caaacaacac
     4681 gattgacaat caagtcaaag atggacaaaa aatgattgtt ttttcattga ttattggact
     4741 tagcgtaggc atcataggcg ccgtttttct tgcccaaaaa atgaaaaaag tactgcttgg
     4801 acttgaacca caagaaatag cagaacagtt aagtgaaaaa acgattatcg aaaatgaagt
     4861 agatgaagga atcttagcca tctcttctga taaaactatc ctcttgatca atacacctgc
     4921 aaaaaacatt ttctaccaaa cagagcagct attggatttc gaagtaggaa aaaaatcagt
     4981 gataatctat atgacgtttt gtttaaagag acgattgagc atcaaacgac cgttcggact
     5041 caatccatgt atttaaatgg gatagaaact atttctagcg ttacacctct ttttagcaat
     5101 gaccgttttt taggagcagt cgcaacttta cgaaatcgat cagaagtaat gaaattaatg
     5161 gaggagcttg gggggacgaa acaatatata aactccatga gagctcaaac ccatgaattc
     5221 atgaataaac ttcatgtcat ttctggtttg attgatttga agaagtatga agaagtcacc
     5281 cattttatcc agcagctaaa tcaaaattat aaaaaagaga ttggccaagt ctcaagtctg
     5341 atcaaagtac ctgcaattgc cggtttttta ttaggcaaaa taaacgaagc caaagaacaa
     5401 tctgttgaaa tgctgttaga aagcgattct tttatcccta aaatggagat ggatgactcc
     5461 atccattcac ttttgcaagt gttagggaac ttacttgata atgcaaaaga agccgtgcta
     5521 caaaaacatg aaagaaacgg acaggtccgg attcatttaa actatgacac agaagctgaa
     5581 gtattcatcg tgaccgtctg cgatgacggt gtcgggatat cagaagaaat ctcagaaaaa
     5641 atatttacgc taggtttttc gacgaaggga atcaatcgtg ggtttggatt gaatctcatc
     5701 cattcaatcg tcaaacgcca cggtggattt ttagaatttt ccaaacaaac aaatggcggt
     5761 acaatcgtct atctagaatt accaaaaaag gaagatcaaa atgatgacaa atgtcctgat
     5821 aatcgaagat gatccaatgg ttgcagaatt gaatcgtact tttgtggaaa gcatccctaa
     5881 tatgaacgta atcgaaagtt tcagaaatgt agaagaagga aaaaaatatt tcagacaaaa
     5941 aaacgcagat ctgctgctgc ttgatgtata catgcctggc gaaagtggta tccatttttt
     6001 atcttggctt agacagcaag gaaatgatat cccagtgatt ttgatcactg ctgcagatga
     6061 tatcgaaacc ataaagaatg tgatccgcta cggtgcctta gattatttga tcaaaccgtt
     6121 cactttgcaa cgttttcagc tggcgatcga aaagtatctt gctttctctc agacaatcca
     6181 aacgactgaa aaagccgatc agaaattgat cgatcaagta ttcaaccaca ccactcccat
     6241 tcacgaaata ccatcattca tcgatttgcc taaaggtctg tcaaaactga ctttaaaaaa
     6301 aataagtgca tttattctaa aagaagatac tattttttca acaaaagaac tggctcaaaa
     6361 aagctcactg tcacgtatat ccgtcaagaa atatttgaat tttttagtag aacaagatat
     6421 attgaacgaa agaatcaaat atttggaaat cggccggccg ctaacctgtt atgaattgac
     6481 aaagaatggg cggaaaaagc tcacagatta ctgttgagtc atcgaattat cccatacaaa
     6541 atagtccaag tatacagaag taacaactgt ttacttggac tatttttaaa cattttattg
     6601 acgaatatcc atgttttctt atcttaattg acgggcttta ggaagagata cctcattttt
     6661 cgtttttcca gtttctatta tttcacgaaa gttctcgaaa cttgtcgaaa tcatatttga
     6721 taatgcctca tctgtatttg aacctacatg cggggttaca agaactctag ggtacatttc
     6781 tactaattgc tggatagcag ggtctggaat cgtctcccat ggcttgaagg atcggaaaaa
     6841 aacctctgtt tcatttgcaa aaacatcggt tgcaaaacca gctaaatgat tgcttttcaa
     6901 agctcttaaa atagcttcat tatcttgaag ctctccacgg gctgtattaa tcaagatgct
     6961 accttttttc atttgagcta aaaatccttc attgatcatt tgatcgtttt cacctgggat
     7021 atgaggtaca tgaatactga caatatcgct tttggctaat aactcttcaa gcgagtggaa
     7081 cgtcaatact tttttagcag cttctgtctg atgaatatcg tagccgatca ctttggctcc
     7141 tagtccctta aacaaaacag cttccgtcaa ccctatcctg cctgttccta tgatccctac
     7201 tgtgcaattt ctgatttcac gactaaacat ttgctcatcc actcgaaaat ctttgaaaga
     7261 tgttcggatc gtcgtatacg cagtatttct caaaagcatc atggcaaaag tcaaagataa
     7321 ctctgctatc gcatttggcg aatacgatgg tacacgggct actgtcatac caaaatcagc
     7381 tgccgcttgt agatcgatat gattgactcc cacagtccgt gtaaaaacat aacgaatccc
     7441 atattcctgc atttttgcaa gattggtctc atctgctata caatttcccc tcaacaatac
     7501 agcatcatgg tcctttgctg acacgatatt gtcatgtgtc aacagttctt cataaagact
     7561 gagatcataa tggtaaatat tcaatttttc aaaaaaatct atctcgttcg gacgaacacc
     7621 gtaacatgcg attctcatac tttcttcctc catttcttat gaaaataaac ctatgccatg
     7681 aatcatttgt aatacaacga tccatacagg tgtcagtacc acagcaaaaa tagttgacag
     7741 taaggatgca ttggaggcta aaagtgcctc cctatcaaaa ctgatagcat aggctgctgc
     7801 cactgttgca gctggtgtag ccatcatgat aacgattgtt gccagtgctt caaaattgac
     7861 tggtagaata ttcgttgccg tcaaaactgc tagaatcacg atattcaatg caggtacaaa
     7921 taccactttg actagactgt aataccatga agttttttca gatgcagctt ttttgaagct
     7981 tacttcacct aatgttgcgc cgattgctag ccacgccaat ggtgaactta aaccagctaa
     8041 ataatccatc ggctgaaata gccacggtgc agtctggtca attcgcaaaa acgctacttg
     8101 gcttatctct ccttctgcac ctgtaactga cacttgtggc agataccctt ggaacagcca
     8161 aatgaaaagg ccggcaaaag tagcgataat gattggattg acgaacatcg ctttgatatt
     8221 tttagcttcc atcttcaagc cactcatttt tatgtatcca taagaataca aaaagattcg
     8281 gtagccaata ttgaaaattg aggaatacat cacgccgaca ggaccataaa tcgcactgac
     8341 gataggcgtt ccgaaaaagg tcgtagaacc aaagatcgtc aggacacgca acgttgtttg
     8401 cttatcccct ttatagctta agaaaaaagg ttttgaaact agaataagaa taatataaat
     8461 aactaatccc caaatcaaga cattgattcc ttgcatcagt aattctgtat tgatatcttg
     8521 catgaaagct cgaaatgcca gtgctggcaa agccactgtt aagacgactt tcgatagaat
     8581 ttttccaaca gattcagaaa aaatcccctt ttttctacaa acaaacccta ataatataat
     8641 aaaaactgtt gaagtgattg cactgacaat cgacatatct gtcaaagtcg atcgaaaaat
     8701 gtcaaataag ttcatctttc tcctcctaat tatgttcatt tattcacaat aaaaaaagct
     8761 tttattttgc taatacgtat taacattttt aattttatct ttacattgtt acttattgaa
     8821 caataggaaa ttaaagaaac ttttgaaact tatgtaaagt gatttttact agaacacaaa
     8881 aaaaacctta aaac
//
LOCUS       DACCQI010000110         8624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCQI010000110 DACCQI010000000
VERSION     DACCQI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.114"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(51..1517)
                     /locus_tag="IVO76_002291"
     CDS             complement(51..1517)
                     /locus_tag="IVO76_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP7430230.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="IVO76_002292"
     CDS             1904..2500
                     /locus_tag="IVO76_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7430231.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="IVO76_002293"
     CDS             complement(2558..3040)
                     /locus_tag="IVO76_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430232.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="IVO76_002294"
     CDS             complement(3146..3343)
                     /locus_tag="IVO76_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430233.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="IVO76_002295"
     CDS             3638..4396
                     /locus_tag="IVO76_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP7430234.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="IVO76_002296"
     CDS             4456..4884
                     /locus_tag="IVO76_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430235.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="IVO76_002297"
     tRNA            4930..5002
                     /locus_tag="IVO76_002297"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="IVO76_002298"
     CDS             complement(5152..5523)
                     /locus_tag="IVO76_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7430236.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="IVO76_002299"
     CDS             5738..5923
                     /locus_tag="IVO76_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430237.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="IVO76_002300"
     CDS             complement(6006..6194)
                     /locus_tag="IVO76_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430238.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="IVO76_002301"
     CDS             complement(6218..6481)
                     /locus_tag="IVO76_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430239.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="IVO76_002302"
     CDS             6639..6896
                     /locus_tag="IVO76_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7430240.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="IVO76_002303"
     CDS             complement(6967..7158)
                     /locus_tag="IVO76_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430241.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="IVO76_002304"
     CDS             complement(7299..7619)
                     /locus_tag="IVO76_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430242.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="IVO76_002305"
     CDS             complement(7642..8076)
                     /locus_tag="IVO76_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7430243.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8411..>8624
                     /locus_tag="IVO76_002306"
     CDS             8411..>8624
                     /locus_tag="IVO76_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7430244.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSG"
BASE COUNT         2733 a         1703 c         1366 g         2822 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ccgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 atatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tctgttacaa aaaacgtgga tttgtcataa attgtcatgc ttcttttacc tatgaaatat
     8341 tttcacgtgt taaagtattc ctcgttgaac acaacaaata aaacttttta acgaataaag
     8401 gagatttttc atgacatcac ttaaaacttt actttttgga acaactttgg ctgccggcgc
     8461 tgcattcttc atgggaacga ctgctcatgc ggacgaagct tataccgttc aatcaggtga
     8521 tactctatca acgatttctc aaaaatacgt tggtgataac tccttgatca atgccattgc
     8581 agaatcaaat tcaatttctg atattaacct gatctactca ggac
//
LOCUS       DACCQI010000111         8622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCQI010000111 DACCQI010000000
VERSION     DACCQI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.115"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            4..1960
                     /locus_tag="IVO76_002307"
                     /pseudo
     CDS             4..1960
                     /locus_tag="IVO76_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
     gene            1950..3545
                     /locus_tag="IVO76_002308"
     CDS             1950..3545
                     /locus_tag="IVO76_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7430245.1"
                     /translation="MKNKWCKSAVIYQIYPRSFQDTNGDGIGDIPGILSRLDYLENLG
                     INAIWLSPVYESPNDDNGYDISDYRKIGAEYGTMADMDLLIKEARQRGIRIIMDLVIN
                     YTSDRHPWFQLAKKSVTSPYHDYYVWRKEPNGLESNFGGSAWTYVPEVNQYYLHLHSK
                     KQPDLNWQNPKMRQAIYDMMNFWLEKGIGGFRLDVIDLIGKQPEQSITKNGPLLHDLL
                     QEMYQKTFGHYDVVTVGETWGATPKIAQLYSKEERKELSMVFQFEHINLDKQNGKRKW
                     DLKDLDPQELHRTFSKWQIELGGCGWNSLFWNNHDLPRIISRWGDDQEYRTISGKMLA
                     IYLHFMQGTPYIYQGEEIGMINYPVKSIDEVDDIESQRMYAERIKKGYSKEELIQSIN
                     SKGRDNARHPMQWNNHKNGGFTEGTPWLPVGNTEEINVEAALAAQDSLFYTYQKLIEL
                     RKELPVIVYGKYQVIETEEPNVLAYLRSLKKQKILVVVNFSDQKTTFPIGKYRVKQVL
                     IQNYKKKITNNLLPYEAFSALIY"
     gene            complement(3740..4075)
                     /locus_tag="IVO76_002309"
     CDS             complement(3740..4075)
                     /locus_tag="IVO76_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7430246.1"
                     /translation="MGIEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
     gene            4361..6241
                     /locus_tag="IVO76_002310"
     CDS             4361..6241
                     /locus_tag="IVO76_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7430247.1"
                     /translation="MKFQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPLTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDDVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVVIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            6320..6547
                     /locus_tag="IVO76_002311"
     CDS             6320..6547
                     /locus_tag="IVO76_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP7430248.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDKVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            6552..7094
                     /locus_tag="IVO76_002312"
     CDS             6552..7094
                     /locus_tag="IVO76_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7430249.1"
                     /translation="MNIVEEKFAQEIKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLLASTILENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            7339..8058
                     /locus_tag="IVO76_002313"
     CDS             7339..8058
                     /locus_tag="IVO76_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7430250.1"
                     /translation="MKKRILLIMGLAFLLAGCTNGNEPRKESTKETAKTTIATTTTTS
                     TESKSTATSTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KAAQLEAFMTTWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFDQIVYGQVNQ"
     gene            8388..>8622
                     /locus_tag="IVO76_002314"
     CDS             8388..>8622
                     /locus_tag="IVO76_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7430251.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPL
                     LVFFLTMASIAKHKIGNKTFVKPILVLYLVGTLL"
BASE COUNT         2824 a         1413 c         1853 g         2532 t
ORIGIN      
        1 gaaatggatt acaaaaaagt agctgaagaa atcagcaaag cgcttggaga agacaatgta
       61 caagcagctg cgcattgtgc aacacgttta cggcttgtct taaaagattc agacaaagta
      121 gaccagaagg ctctggatga taatgaaagt gtgaaaggaa cttttgaagc aaatgatcaa
      181 tttcagatca ttatcggagc aggtgatgtg aataacgtat atgatgaatt cgttaagatc
      241 accggcgtaa aggaagcttc aaccgacgat ttgaaagcgg tagcatcgaa taagaagaat
      301 aatactgtca tggcctttat caaggtgttg tcagatatct tcgttccgat tgtccctgca
      361 ttggttgcgg gtggactgtt gatggctctg aataatgttt tgacggcaca ggatttgttt
      421 ggtcctcagt cagtggttga gttgtatcca tcagtgaaag attttgctgg catcattaat
      481 ttacttgctt cagcgccgtt tgcttttttg ccgattttgg taggtttttc agcaactcga
      541 cgttttggag gcaatccgta tcttggagca gcaatgggga tggcgatggt catgccggat
      601 ttagtgaatg gatacagtgt gacgaatgct attgcagaag gatcgatgcc ttattggaat
      661 atttttggaa tgaatgtggc acaagccggt tatcaaggat ctgttcttcc agtccttgca
      721 gtttcttgga ttttagctac tttagaaaaa ttcttccata aacggttggc taatgctttc
      781 gactacacat ttactccaat gctagctatt attattactg gttttttaac ttttacagta
      841 gtagggccgg tgatgcgtac tgtcggagac ggcttagctg atggattaag ctggttatac
      901 agtactactg gagctttcgg cttaggtgtc tttggtttct tctattcgcc gatagtcatt
      961 accggattac atcaaagctt tccagcaatc gagactactt tactagcaga tgttgccaga
     1021 acgggtggct cttttatctt tccagttgcc tcaatggcaa atgtagcgca aggagcggct
     1081 gctttggcga ttatgctgct gacaaaaaat aaaaagcaaa agagtctagc taccactgca
     1141 ggtgtttccg ctatgttggg gattactgag ccagcgatat tcggtgtgaa tttgaaactg
     1201 aaatttccgt ttatttgtgg aatgattgct gcaggaattg cttcattctt tatcggcttg
     1261 ttccacgtat tagcagttgc gatgggaccg gcaagcgtca ttggctttat ttccattgca
     1321 cctcgttcga ttccttcatt catgatcgga attgttatca gtatagtgat cggttttttt
     1381 aagcacttac ttctatggga aaaaacatat ggaactgcta gcagatggtg aatcaaaagg
     1441 aacagaagca gctgagtcaa tcagttcagc tcctaaaacg actaggaaac cgcaagatga
     1501 aattttagct gcatcagtca gcgggaagat tctcccttta acgaaggtaa aagatccagt
     1561 tttttccact gaaatgatgg gaagaggtat agcagtcaaa ccagatgacg gggtagtcca
     1621 tgctccagca gatggtatat tgacagttgt ccacgaatct aaacatgcat atggcttgaa
     1681 aacagagaaa ggagcggaag tactgatcca tatcggtatc gatacagtga atctaaaagg
     1741 ggccggattc acgacacagc gacaatatgg tgaattagtg aaaaaagggg atattctcgg
     1801 tacatttgat cgtaaattgc ttaaagaaga agggtacgac gatacaatta tggaaattgt
     1861 cactaattcc tctgcatatg ctaatgtaaa aattcttgaa aaaaacacgg tcaaaagtgg
     1921 tgaagatatt ctgatagtga gtgaaacaga tgaaaaataa atggtgtaaa agtgcagtaa
     1981 tctatcaaat ctatcctcga agttttcaag atacaaatgg agatggcatt ggagatattc
     2041 ctggtattct ttcaagatta gattatctag aaaacctggg aatcaatgcg atatggttaa
     2101 gcccggttta tgaatcacct aatgatgata atggctatga tatcagtgac tacagaaaaa
     2161 taggcgcaga atatggaacg atggcagata tggatttact gataaaagaa gcacgacaaa
     2221 ggggaataag aatcatcatg gatcttgtta tcaattatac gtcagacaga catccatggt
     2281 ttcaattggc aaaaaaaagt gttaccagtc cctatcacga ctattatgtt tggcgtaaag
     2341 aaccgaatgg attagagtcg aacttcggcg gttcagcatg gacctatgtg cccgaagtga
     2401 atcaatatta tctgcacttg cattcgaaaa agcagccgga tttgaattgg caaaatccaa
     2461 agatgagaca agcaatctat gacatgatga atttttggct tgagaaaggg attggcggat
     2521 ttcgattaga tgtcattgat ctgattggaa aacagccgga acaatctatt actaaaaatg
     2581 gtcctctttt acatgattta ttgcaagaaa tgtaccaaaa aacttttggt cattatgatg
     2641 tagtgacagt gggagagact tggggagcaa cgcccaagat tgcgcagctg tattcaaaag
     2701 aagaacgcaa agaattatca atggtctttc aattcgagca tattaatcta gacaaacaga
     2761 atggcaagcg aaaatgggat ctaaaggatt tagatccaca ggaattacat cggacttttt
     2821 ctaaatggca gattgaatta ggtggttgtg gttggaattc tttattttgg aataatcatg
     2881 atctcccacg aatcatttct cgctgggggg acgatcaaga atatcgaaca atcagtggaa
     2941 aaatgctagc gatatacctc cattttatgc aaggtactcc ttatatctat caaggagaag
     3001 agatcggcat gatcaattat ccagtaaaga gtattgatga ggtagatgat attgaaagcc
     3061 aaagaatgta tgcagaacgt ataaaaaaag gctattcgaa ggaagagttg atccaatcaa
     3121 tcaactcgaa ggggcgagat aatgctcgcc atccgatgca atggaacaat cacaaaaatg
     3181 gtggctttac ggaaggcact ccttggttgc cagtggggaa tacagaagag atcaatgtag
     3241 aagcagcatt agctgctcaa gattccttgt tttatacgta tcaaaaactg atcgaattgc
     3301 gcaaagaatt acctgtgatt gtctatggga aatatcaagt cattgagaca gaggaaccga
     3361 atgttttagc atatctacgc agtttaaaaa aacaaaaaat actagttgta gttaattttt
     3421 ctgatcaaaa gactactttt ccaatcggta agtatcgtgt caaacaagtc ttgatccaaa
     3481 attacaaaaa gaaaattact aacaatttac tcccatatga agcttttagc gcgctgatct
     3541 attaagtggt tttttctata gatgaatctt tgttatgagg tagatagttc aatgaaacag
     3601 ttattcgatt tgtttataga aaacaattta atcagcaaaa agggggacaa acttttaatt
     3661 gtctcccttt aattgttagg atggaatatt atcactctta aacaataagt taaaaaaata
     3721 ctcgtttaca taaaaaagat tatttagatg cgataatatc atagataaag aaacattcat
     3781 cataagaaac agtgatccca aattctgttt caatattttt gaaggccttt tttacatgaa
     3841 aaattgtttc atgagaaggt cttgtgtcat cttctgatat aattttaagt tcatgaccgt
     3901 ttatcaatgt acgctcaatt agacacgctg tatgaatcaa aagttttaaa agaatggaat
     3961 gagtgaattt tttgtggaaa gtacattcca attcttggat aaaatccatt aagctttttt
     4021 ggatcttatt cccagctaat aagtagagat atttgtccac tgcttcaatt cccatatcaa
     4081 aaaacgatat ttcttgtgaa tgagtggctg ctgccctttt ttgattgttg ttcttcagtc
     4141 gtaagatgac aaagcatctt atctttgaat aaaaaaatag aactacctaa ggaagcgttt
     4201 cggcgcttcc tttttctata tgatcgttta atgactgtag gaattcagta taccaaaaga
     4261 aaaccacctt tttgactaaa cttgatagtg gtcaattttt tatgatggat aagtatctat
     4321 acttagtctc gtaagaatga aaaggaaaag agggagaaag atgaaatttc aaaagtggat
     4381 ccaagagcat cgaaatcata tcaccgcctt atcagctgtg ttgattattt ttggttttat
     4441 cggaaaattt actggatttg tcttgttcta taacattgct ctagccatag cttctattat
     4501 tgcggtcata cctattatga tccatgcgta tcaggcagta aaagtcaaag ttatctcaat
     4561 tgatttactc gttactattg ctgtgcttgg tgcctttatg atcggagaat acaaggagtc
     4621 agctatcgtt acattcttgt ttttgtttgg tagttatctg gaacaaaaaa cattgaccaa
     4681 aacgcgggaa tcaatcaagg ctttgaccga gatggcccca ttgactgcca gcgttgtcca
     4741 agaagatgga acgactgaag aagtggaaat cgatgacgtg gaagaaggtg atatcgtctt
     4801 agtaaaaact ggtggagcta ttcctgtgga cggacgagtc attgacggaa aagggtatgc
     4861 ggatgaagcc gcagtgacgg gtgaatcacg acaagtcgtc aaaatcagtg gagatgacgt
     4921 attttctgga acgattttag cagatggcta cgtgacgatc gaagcaacta aggtcggaga
     4981 tgatacgacc tttgccaaaa ttatcgaatt agtcgaagaa gcacaagata ccaaatcaca
     5041 tgcggaaaaa ttcattgatc gtttttctca gtattatact ccaaccgttt tacttattgc
     5101 gttgcttgtt ggtctgatca gtcgtgattt tcgtttagcc atcactgttt tggttcttgg
     5161 ttgtcctggc gctttagtca ttggtgcgcc tgtttcgaat gtagcaggaa tcggtaatgg
     5221 agcgaaacgg ggcatcttag tcaaaggcgg tgaagcaatg gatacttttt ccaaaatcga
     5281 tacttttgtc tttgataaaa ccggtacatt gaccaaagga actactgctg tatccactat
     5341 caagaactat acagaggatg aaggggacag cttagcttta gtggctcgat tagaaacgtt
     5401 atccgatcat cctttggggc gcgccgtgat ccattatgct caggagaaag acattgattt
     5461 tcaacaatta gcagtcaaag ataatcagac gatcaaggga caaggaatca cagctgagat
     5521 tgatggtcat acagtttgtg cgggcaacac caaattgatt gccgctcaca aactttctct
     5581 cacaccaaag caagtacaag atttacatca attacaacaa acaggcagct cggtcattat
     5641 tgtagtaatc gacaaaaaaa tcactcaaat tattggtgtg tcagatgtga ttcgaccaga
     5701 agtcgctgaa caattagcta tgttgaaaca agcaggagca aaacatttag tgatgctgac
     5761 aggtgataat cagatgactg ctgattatgt agctgatatg ttaggcattg atgaagtcca
     5821 tgctgaatta ttaccagatg aaaaggttca atttgtgaaa aaataccaag aacaaggatt
     5881 gcgcgttgct ttcattggag atggaatcaa tgattctccc tctttggctg cggctgatat
     5941 cgggattgct atgggatcag gaacggatgt tgctatcgaa acgagtgacg ttgtcttgat
     6001 gcaatctagt tttggatcgt tggttcatgc ctaccgtttg gctaagaaaa ctgttttgaa
     6061 tacccgtgaa aatgtagtga ttgccattgg tgtcgtctta tttttactca tcggcttatt
     6121 tgccgggttt atttacatgg ctagtgggat gttcgttcac gaagctagta tcttgatcgt
     6181 tatctttaac gcgatgcgtt tattgtattt tggacacgaa agcaaagagg aaaaatcgta
     6241 aaacttgata agaatcaatt tttttatgga agctgtaatc tagaataaga ctatcaagaa
     6301 aaagggagga agaactataa tgaaaaaaat gattatgcaa ttagatacac tagcttgtcc
     6361 aagctgtatg caaaaaatcg aacatgcggt ttcgcaacag cctggggtaa gcaatacgaa
     6421 agttttgttt aatgcgagca aaatcaaagc aaactttgat ccagataaag ttcaccctga
     6481 acaattagct gcagttgtgg aaaaattagg ttatgaagtc caatcaacaa aagtaaaaga
     6541 ggcataaatc aatgaatata gtggaagaaa aatttgctca agaaataaaa caagcagata
     6601 tcgaccatca taaaccaact gctggcgcca tggtggggca tgtattagca aatttcaaat
     6661 tagaagaaat gaaattgatc caagcacaat tgtatgtttt gggtacatct tcgcaagaat
     6721 taagtaccct tttttcagaa tatgctgatc aagaagcgag atggttcaat aaaatctctc
     6781 atgagttagt gattgagaat gaaatcgttc cgtctacttt agaagaagtc atgcgttatg
     6841 gaaaaattta cgaacatggt caaaacaaat atttactagc ctcaacaata ttggaaaact
     6901 ttgcaaaaga ttttgatttc caaaatgtct ttatcacacg ggcaatcaag ttagctgaaa
     6961 aagaagaaaa atatggcttg caacaattac tcgttgaact tttaagtttc aataaagaaa
     7021 tcatctacca agtacaaaga ctccaaggaa aaactgtccg acaagatttg gatgaggaag
     7081 acgatgatga ctaaaagtct atgtttcttc aaacacaaat ataaagcgtg agaaaagcag
     7141 gaaaaaaaca cgaagttttt gtcttaaaaa taaaataaaa agaagcaccc gctgatgttg
     7201 tctataagac atcagcgggt gcttcttagt ttttaaatgg tttaaaagat ggatgagaaa
     7261 atacaaaaaa tcaataaaat cacattgctt atttgataga ataaagaagt gggggagtaa
     7321 tcgaaagggg ttttttaatt gaaaaaaagg attttattaa taatgggtct agcttttttg
     7381 ttagctggtt gtaccaacgg gaatgaacca agaaaagaat caacaaaaga aacagcgaaa
     7441 acaacaattg caactactac aacaactagc acagagagca agtcaactgc aacaagtaca
     7501 gaaacagcag atacctctac ttcgtcagag tcttccaaaa cgactgaaac aactcaaaca
     7561 tctacttcaa gttcttctgt agaggcttca tcttcctctt cagaaagcag cgaacaagtc
     7621 gaacaaacac tgtggaatgc agacaaagcc gcacaattag aggcatttat gaccacatgg
     7681 ggccaaacaa tgggacaaca atacaaaagt tatacgaatc aaatgagtgt ggacttatat
     7741 ggtcttaaag tccctcaagc tattttgaat ggggagtgga agatggctat tggtggagtg
     7801 cctgtttctg cagaatggtc agaaagcggg acaggtcaag cagattatca aatcacagcc
     7861 gtctattccg atgcagagac agaaccatat ctgaaaaagc acgtctatct attcggtttt
     7921 cagcaaaatc agccaaaagt attggtgaca caacaaaatc aggggaatcc cgataattat
     7981 ctttacttca acgagacagc gaataatgaa ttgaaaaatg gctttgatca aatcgtttat
     8041 gggcaagtaa accagtgaga gtgagagaaa gaagagactc agtggaaaat attaattgat
     8101 tgttatgtca aagaattaag atgaaggatg aaatttctag tttcgtcctt catcttagtt
     8161 acgttttact tggataactt taataataac gataaatagt tttctctcga cttctttaga
     8221 gattgaataa accctccaat actaacattg aatgttgcaa gagcatgaat tgccaattgt
     8281 attattagaa tttttagaaa attgcacctt tagtttcttt agataaaatg atacaatgat
     8341 tggtaaaatt gtgaaagggt ggtcatttgt tagagaagtt tttggatatg tcgttagttc
     8401 aacgtattgt tgttggtatt ttagtcggaa tattgtttgg tatctttttc cccacgtgga
     8461 catttatttc aatattgggt acgttgtttg tcagttgctt gaaagcaatt gcgcctcttc
     8521 tagttttttt cttgacgatg gcttcaattg ccaaacataa aataggaaat aagacatttg
     8581 tgaagcctat tttagtctta tatttagtag gaacattgct tt
//
LOCUS       DACCQI010000112         8335 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCQI010000112 DACCQI010000000
VERSION     DACCQI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.116"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            264..1457
                     /locus_tag="IVO76_002315"
     CDS             264..1457
                     /locus_tag="IVO76_002315"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7430252.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1587..3059
                     /locus_tag="IVO76_002316"
     CDS             1587..3059
                     /locus_tag="IVO76_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7430253.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3142..3930
                     /locus_tag="IVO76_002317"
     CDS             3142..3930
                     /locus_tag="IVO76_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7430254.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3997..4665)
                     /locus_tag="IVO76_002318"
     CDS             complement(3997..4665)
                     /locus_tag="IVO76_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7430255.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4780..6492
                     /locus_tag="IVO76_002319"
     CDS             4780..6492
                     /locus_tag="IVO76_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7430256.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6495..8267
                     /locus_tag="IVO76_002320"
     CDS             6495..8267
                     /locus_tag="IVO76_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7430257.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2551 a         1391 c         1828 g         2565 t
ORIGIN      
        1 gaagatactt tttgagtatc tttttctttt tatctgaaag aatgatttta aaaagcttag
       61 cagagttatc tttatggatg attgtgctaa gcttttttgg acagttctaa tgtaaaatat
      121 ttttatattt tttaagcaaa aaaatgaaaa aatacgagca gaaaactgtt ttatctgcta
      181 tacttggttc gttgcaagtt cccgaaagga tccgcaggac ggagatgcct tgtaaccgaa
      241 tttaactagg gggaatcatt ataatggtag aaagacactt atttacatca gaatctgttt
      301 ctgaaggaca tccagataaa attgctgacc aaatcagtga tgcaatcttg gatgcaattt
      361 taaaacaaga tccaacagca cgagtagcgt gtgaaacgtc tgtaacaact ggtcttgttt
      421 tagtatttgg agaaatatct acgacggcat atgtagatat tcaaaaagtc gtacgggaaa
      481 caataaaaga aattggttat acacgtgcaa aattcggatt tgatggagat acagcagctg
      541 tattggttgc gattgatgaa caatctcctg atattgctca aggagttgat gaagcacttg
      601 agatccggga tgaagataaa aaagacgtat tagatgagat tggtgctggt gaccaaggtt
      661 taatgtttgg gtttgctgta gatgaaacac cagaattgat gcctttacca atcgctttga
      721 gtcatcgtct ggtacgacgc ttggcagatt tgcgtaaatc aaatgaatta acgtatttac
      781 gtccagatgc aaaatctcaa gtaacggttg aatatgatga tcaaggacaa ccggaacgcg
      841 tagatacaat cgttatttca acacagcatg atgatgcagt agataatgaa acaattcgtc
      901 atgatgtcat cgaaaaagta gtgaaggaag ttattccagc tgaattatta gatgatcaaa
      961 cgaaatatta tatcaatccg actggccgat ttgtcattgg tggtcctcaa ggggatgccg
     1021 gattaacagg aagaaaaatc attgttgata cgtatggcgg ttatgctcgt catggtggcg
     1081 gtgctttttc tggtaaagat gcgacaaaag ttgaccgttc tgccagctat gctgcacgtt
     1141 atattgctaa aaacattgtt gcagcagggc ttgctcgcaa agcagaagta cagctagctt
     1201 atgcgattgg tgttgctcaa cctgtttcga tctcaatcaa tactttcggt actggaacgg
     1261 ttccggaaga agaactgatt gctgcagtga gagaaaactt cgatcttcgg ccagcaggaa
     1321 tcatcgagat gcttgatttg cgtcgtccga tttacaaaca aacagctgct tacggtcatt
     1381 ttggtcgtac agatgtagat ttgccttggg aacatacgga caaagtagat gcgttgaaag
     1441 caagtttagc aaaataagtt atattcagct ggaacgatcg ccaagtggtg gttgttccag
     1501 ctttttttat ggcagttaac ctcttgaaaa agatagtaat tatttatcta tttttagatc
     1561 ctgtcataat gaaagggagg aataagatga aaaaagaaac aaatgttact ttggtcacag
     1621 tgagtatttt cattgcaacg ttcatgacgg ctattgaagg tacaatcgta acaaccgcta
     1681 tgccaacgat tgtcggttca ttacatggaa tggaaatcat gaactgggta ttttcgattt
     1741 atttgctgac aaacgctatg cttactccga tttacggaaa acttgcggat aaagtgggac
     1801 ggaaaccaat ctttattttt gggacattat tgtttattat cggttcgtta ttttgcggtt
     1861 tatctcaaag catgattggg ttgattgctg ctcgtgcgat tcaagggatg ggtgctgggg
     1921 caatgatgcc agtagctttg acgatcatcg gcgatcttta ttctattgaa aagcgagcaa
     1981 aagttttagg tttgaatagt tcagcctggg ggattgccag tgtttttgga ccgcttgcgg
     2041 gaggattcat cgttgaaacg atcagctggc actggatctt ttttatcaat gtgccgatcg
     2101 gaattctatt aattattttg atttggctat atttaaatga accaaaaatc atacatgagt
     2161 ctaaaccaat ggatattttt ggtagtttta gtttgatggc catgcttgtc tcattgctgt
     2221 tagggttcca attattagga gacgaaggaa tgagtccgct tgtttctgct ttgctgctag
     2281 gtagtatcgt aatgttaggt atttttgttt ttgccgaaaa aagagctaaa gatcctgtca
     2341 tcatgatgca tttattcaaa aatccactat ttgttgtggt caacttggct gcagcattgg
     2401 ctagtggttt tttgatggga atcgatgttt atatccctat gtggatgcaa ggtgtattag
     2461 ggctgaatgc agcaattggt gggctggtat tagcaccatt atcactagtc tggatggtcg
     2521 gttctttcct tgcaagcaag tggatgactc aaatgaaaac tcattgggtc ttgagaattg
     2581 gactactaat cgcagctatt gggggcgctt ggttgtatct tatgcctttc agaagtaact
     2641 atttatggtt ttttgctata tcaacggtca tcggtatagg cttaggtgtg accatgacta
     2701 cctcgactgt cagtgcacaa aatagtgtat ctcgtgacca gcttggtgta gcaacttctt
     2761 ttaatacgtt agttcgaaca attggtcaaa cagtcatggt ttctattttt ggtttattga
     2821 taaatggggt aactaaacga gaacttgcag cagcaaattt aacaaatgat gcagatatca
     2881 tgaataaact ggtgaatcca caaacggcta aatatattga tgcggatatt ttgttaccat
     2941 taagaaaaat tctttatgat gggttacatt ccgtttatct agttggcttg ttactcgtga
     3001 tcattgcttt ggtcttaacg cttttcgttc atgaaaaaag tggtaaaata aaacagtagt
     3061 ttcggttaag atatgtacgt agccgaatca ttcaaattta aagttattca aaaataactt
     3121 cttactaagg aaggcgaaaa tatggcattt attcaagcaa atatttattc gaatgtgtta
     3181 gaaatggaag tcaatctaaa tgtgatacta ccgcaaacaa caaaaaaggt gattggcacc
     3241 agttcagagc aagagacaac ggatatacct gttttatatt tacttcatgg aatgggtggc
     3301 aatcatagtg tatgggaaag acgtacgtcg attgaacgtt atgtagcaaa ctacggatta
     3361 gctgtcatca tgccatctac tgatcttggc tggtacacag atacagttta tgacatgaag
     3421 tattggacat tcattgcaga agagctacct gagatttgtc atgaattatt cccgcaatta
     3481 acaagaaaaa gagaaaaaac atatgccgct ggattatcga tgggcggata tggcgcgtta
     3541 aaactaggcc ttgcaaaatc agaaaatttc gcagcagtaa tctctctttc aggcgctgtt
     3601 tctgtaggaa atcgaatgga tgatcttttg atggttcgta aagcatcttt ttgggaaggg
     3661 atctttggac cattggatca aatcgaaggt tcaaaaaatg atccgcaata cttattgaaa
     3721 gaactagtaa aaagcggaaa accagttcca agattttata tggcttgtgg ggaatctgat
     3781 tttctttatc aggcaaatca ggaaatggta tctgctatgc aggaacaagg cttggcggtc
     3841 acctttgaac aaggacctgg cgatcataac tggatcttct gggaccaatg gattcaacgt
     3901 gcattggcgt ggctaatgga agaaaaataa aaaaagagtc ttaaaaagat agctcctata
     3961 gagatcacaa acatagtgat ctctatagga gcttttttac tgctcgtttt ctcttctttt
     4021 attttgttgg cataagcctt caatataggt ttgtgcactc gttagaaaga gtgctttttc
     4081 attcaattct gcatgggact gcatactttc tttaaatccc tctaattgat tttcaactac
     4141 ataattacgc ataaatagaa ctgtttcttt caaaaattca tttccgtcca taattttttg
     4201 tcgaagctgc tgttcatgct gctgatccat taactgtcca atcaataaat tgtggagaga
     4261 ggcaatggcc tgatgacttt gggtgactga aaatccttca cgaacaagta attggatcat
     4321 ttgctccaaa tgttctaatc gactcggaaa agcgggcaca gtattgattt ctaattctgc
     4381 cccacaaggg aaacgtgtat aaaggtcata gtaattttcc atatactgca gcaactgttc
     4441 attccaaggc aaagttggat ctggaagcgc taattcttct tccattgttt cagccatttt
     4501 ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa gcaggtgctt gaacatgaat
     4561 tctgtttgcg atgtttcgca tggaaagctg cgaaagagct tgtgtttcag ccaggatatc
     4621 aaaggcggcc tgaatgatga ttgcttgaga aagctttatt tccattttat caacctcgtg
     4681 tattgcatta tctttttaaa catggtaaat tgttagtcgg tctaatttta cagtgttaaa
     4741 ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa tgaaattaat cttacgacat
     4801 gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt ccgtagcagt catggttgca
     4861 gcagcattat ggcaacctaa gttactacag caagtattgg aagctattat tacagaagat
     4921 aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc tggctttgct aggattagcc
     4981 gcaggtgtaa cgaacacgat tttctctgca aaagtagctc aaggcgtaag tgcagatatc
     5041 cgagaagaag catttcgaaa gatccagaca ttttcatttg gaaatatcga acagttctct
     5101 gctggaaatc ttgttgttcg tctaacaaat gatatcacgc aaatccaaaa tttagtgatg
     5161 atttctttgc aatcactttt tcgaattcca ttcttattta ttggtgcctt cattttagct
     5221 atgatcacga tgccgcaatt atggtggatc atcgtagcgc ttatcgttac ggtatttctg
     5281 attacggcac tttcatttac aaggatgggc aagcacttca tgatcattca aaaactgatt
     5341 gatcgtgtga atagcattgc aaaagaaaac ttactaggga ttcgtgtagt gaaatctttt
     5401 gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa gtgaggactt aacaaaacac
     5461 aacatcatag tcggtacatt attttctgtg atgatcccgt ccttcatgct ggctgccaat
     5521 ttggctgtgg ttggggcgat cttttttgtg agcgatcttg ttaaagatga tcctacgtta
     5581 attggtggaa ttgcttcatt catgaactac ttgatgcaga ttatgatggc aatcattatt
     5641 ggcggaatga tgatgatgat gacttcaaga gcagccgtat cgctgaagcg gattgcggaa
     5701 attttagatg ctgaaccaga tcttacttat ttagacgttc cagaacaaga gttgacgggt
     5761 tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg aggatacaga tacgcttaaa
     5821 gatatcagct tttctatcaa acctggagaa atggttggta tcgtcggggc gactggtgcc
     5881 gggaagtcaa cactagcaca attgatccct cgtctatttg atccgacaga aggcaaagtt
     5941 gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga gtttaagaca aacggtttcc
     6001 tttgttttgc aaaaagcaat tttattctca ggaacgattg ctcaaaactt gcgccaaggg
     6061 aaaaagaatg caacagaaaa agagatggaa catgcttctt ctattgctca agcaaaagaa
     6121 ttcatcgaaa agttatctga tcgttacgaa gcaccaatag aagaacgtag cagtaacttt
     6181 tctggtggtc aaaagcaacg tttatccatt actcgtggcg ttatcggtaa tccaaagatc
     6241 ttgatcctag atgacagtac gagtgcactg gatgctcgct ctgaaaagct ggtccgagaa
     6301 gcactcgatc gagatttgaa gggaacaacg acgattgtca tcgcacaaaa aattgcttct
     6361 gttgtcaaag cagaccgcat cttagtattg gatgaaggca gattagtcgg cgaaggaacc
     6421 catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa tctttgaaac tcaaaaagga
     6481 acggaggagt aagtatgact gatctaataa aagcaagtaa atttttctat cactatttga
     6541 agcggtacaa actctccttt ttctttatct ttatcacagt gatcattgct acctatctac
     6601 aagtaaaggc acctcaatac atcggggaag cttttcaaga attagctaat tacgtaggag
     6661 gcttgatgca aggcgtagac gataaaagca aatttgtttc tgtaatatgg aaactcttgc
     6721 tgttttatgt actagctagt gcagccaact ttatctatag tattttgttc acacaagtag
     6781 tagggaagtc aacgaaccga atgcgtatcg gtctattcaa caaattggaa aaattgacga
     6841 tccgtttctt tgattctcat caagacggtg aaattttaag tcgttttacc agtgacttag
     6901 ataatattca aaatagtttg aaccaagctt tgcttcaagt catcacgaat gctgttttac
     6961 ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga gttagcttgg gctacaattg
     7021 cttccactcc agtagccgtt ttgatagctg tcttgattat ccgcaaagca cggaaatatg
     7081 tggatcttca gcaagatgaa gtggggaaac taaatggtta tatggatgaa aaaatcagcg
     7141 gacaacgtgt catcatcaca aatggcttac aagaagaaac gattgaagga ttcttagagc
     7201 ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc ttattctggt ctactatttc
     7261 cgatgatgca aggaatgtca ctggtcaata cagcgatagt tatctttttc ggcggctggt
     7321 tagcattaaa tggtgatttg gaacgttcgg tggcattagg cttagttgtg acattcgtcc
     7381 agtattcaca acaatattac caaccactga tgcagatttc atctggatac agcatgatcc
     7441 aattagcgat tacaggggca agacgattga atgaaatatt tgatgaaaaa gatgaagtca
     7501 aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg tcttgcgttg cagcacgtag
     7561 attttggtta ttctccagaa aaactagtct tgaaagatgt ctcgatcaat gtggacaaag
     7621 gagaaatggt tgctcttgtt ggtccaacag gatcggggaa aacgacgatc atgaatctgc
     7681 taaaccgttt ttacgatgtc aatgatggtt cagtagtcat cgatggaata gatatccgag
     7741 attatgatct tgatgcatta cgtagacatg taggaatcgt tttgcaagaa tctgtattgt
     7801 tctccggaac gatcaaagac aatatcacat tcggcaaacc ggacgcttca gatgaagaag
     7861 tcatcaatgc agcaaaacaa gcgaatattc atgacttcat catgacgtta gaaaaaggtt
     7921 atgatacaga gatatccgaa gaaaataact tattcagtac aggtcaaaag cagttgatca
     7981 gtattgctcg aacgatcatc acaaatccat ctttattgat tttggatgaa gcaacaagta
     8041 atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat ggatgaagca atcaaaggaa
     8101 gaaccagctt tgtgattgct caccggctga aaacaatctt gaatgcagat agaatcgttg
     8161 tcttgcgtga tggggaagtc attgaagaag gcagtcatca tgagttatta gcagaaaatg
     8221 gattttatgc ggaactttat cataatcaat ttgtatttga aagttaaatt aaaagaacct
     8281 cgttataacg gggttctttt tatatatttt atgttgtttg tttttataag gggca
//
LOCUS       DACCQI010000113         8297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCQI010000113 DACCQI010000000
VERSION     DACCQI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.117"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(57..1010)
                     /gene="menA"
                     /locus_tag="IVO76_002321"
     CDS             complement(57..1010)
                     /gene="menA"
                     /locus_tag="IVO76_002321"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7430258.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1023..2126)
                     /locus_tag="IVO76_002322"
     CDS             complement(1023..2126)
                     /locus_tag="IVO76_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7430259.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2216..3151)
                     /locus_tag="IVO76_002323"
     CDS             complement(2216..3151)
                     /locus_tag="IVO76_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7430260.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3453..5387
                     /locus_tag="IVO76_002324"
     CDS             3453..5387
                     /locus_tag="IVO76_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7430261.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5844..6260
                     /locus_tag="IVO76_002325"
     CDS             5844..6260
                     /locus_tag="IVO76_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7430262.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6274..6840
                     /locus_tag="IVO76_002326"
     CDS             6274..6840
                     /locus_tag="IVO76_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7430263.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6863..7849
                     /locus_tag="IVO76_002327"
     CDS             6863..7849
                     /locus_tag="IVO76_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7430264.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8004..>8297)
                     /locus_tag="IVO76_002328"
     CDS             complement(8004..>8297)
                     /locus_tag="IVO76_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7430265.1"
                     /translation="SSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2453 a         1602 c         1673 g         2569 t
ORIGIN      
        1 tttggctgtt cttattactt gatgcagggc agttttttca caacctcttc atgctgttaa
       61 atggcttgca taatgattgt tagtaatagc cccaatacat aactggcatt gaataagacc
      121 atatttttga tggaatagct gaaactgata ggctggggta acgtttgctt gaattgatcc
      181 aaatgcttct tcacaattgg caaggtcaag aaggtgacca agatcggcca ttcatagata
      241 ccgctcaaca accccattaa aatcacgaaa tagcaagcat acatcaatag ctggaataag
      301 actgttcccg caggacgtcc gatataaaaa actagtgtat agcgatgatt ttcaatatca
      361 cggtcaagat cccgcaaatt attagccaac atgatattgg caatcgtaaa caccaagggt
      421 aaggaagcta atacgattgc tgctatagcc caaagatttc ctaccaaatc aaattgtcca
      481 gatgaaaagt tgactgtcag atcatagaca tctgaaccga atgtattgac ataaacgacc
      541 atggcaaaaa ttccaaggcc catagtcacc ccactgaaaa tttcccctaa aggcattcga
      601 gataaaggaa tcggtccgaa tgtatagaat actccaataa aacaacagat aaatcccatc
      661 aaaagtaaaa gccaacccgt attgatcgtt agaataacgc cgataacaaa aacaaatgca
      721 atcatagcta aaatcatgtt tcgaatcaat acaggagata gatttctgcg gccaatgaca
      781 ttttcttcat acttatatac ttcagaatga gccttttcat aatccatgta attattgata
      841 gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt gtttagccaa
      901 tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg aaatacacta
      961 gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat ggttcgtact
     1021 ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga taatttgaag
     1081 gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa gatcgcttcc
     1141 gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc cattgaaaag
     1201 actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt gacacttgcg
     1261 atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta ttttttgcca
     1321 tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt ccgttcttta
     1381 atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac tgtccagtct
     1441 tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa gtcaacgatg
     1501 gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat gtaacctttg
     1561 gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt tttttctttg
     1621 tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat ttcttcttgg
     1681 gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc accaatcgcc
     1741 atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac taacgtgtag
     1801 acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc atcgatctca
     1861 gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc gaaggcaggt
     1921 ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt ccctaacaag
     1981 aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact ttcagtggtt
     2041 gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc cagtgtgatt
     2101 aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa ggtgagacga
     2161 ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta tgtgattatt
     2221 tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca gctgcattca
     2281 ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct gtaacaactt
     2341 ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg tcgttcagag
     2401 tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca ttatattctg
     2461 tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat tcagttactt
     2521 tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag ttcttttctt
     2581 caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt gtatctgtgt
     2641 taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt tcagatgagt
     2701 gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag aattggtcat
     2761 tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg tttttgttgt
     2821 attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat tttgattcag
     2881 tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt gaataatttt
     2941 tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact tttgcagatg
     3001 attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa gaagagtttg
     3061 agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat gctaatacgg
     3121 caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc atttctagtc
     3181 cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata agtcaactat
     3241 ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt tcgtgaaaaa
     3301 acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga acagaacatc
     3361 tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc atatattcat
     3421 tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt gtcgttgtag
     3481 gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg aaaaaagaag
     3541 atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc gagttgcatg
     3601 aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag catttgtttg
     3661 ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat aaagaacgaa
     3721 aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt ggaatgggcg
     3781 gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc ctttggtcat
     3841 ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa gctgcattgg
     3901 aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt tctggattta
     3961 ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc aaagattata
     4021 aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg atcttgaata
     4081 tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac aacgtggaat
     4141 tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg aaagacggac
     4201 gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact tctgacgctg
     4261 ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa tatatgcagg
     4321 caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt tatgaagaat
     4381 tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga cacacagccg
     4441 cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa ggaaactatc
     4501 aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt gataagatcc
     4561 atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa tacttctttg
     4621 atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc catatcaaag
     4681 atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta tggagtgtgc
     4741 cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa atcattggga
     4801 atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc actggaaaca
     4861 gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt gcttctgctg
     4921 ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa gcagcgactc
     4981 aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct gtgattgaga
     5041 agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat gatgtagcaa
     5101 tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca ttgattgctg
     5161 gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg ttctgcttat
     5221 caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca ttgatgaacg
     5281 gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa cgtacactag
     5341 gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata gataaagaac
     5401 gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac agcctccttt
     5461 ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa gacacaggtg
     5521 tcacaacctc tgttacaacc tcttgttata aacggtgttc cagaagtagc ttcttcgaaa
     5581 aataagccga aatttcacaa aaacttgaaa agtaattttc gtaaattcct tcttatttct
     5641 tgaagttgcc cacttctgtc acaacctctt gttataaacg gtgttccacc tgctgcttct
     5701 cggtaataag cccaaagaat tagaaaacat ggaaagctgt tttctattct tcgttcttat
     5761 tactcaaagc aagacacagg tgtcacaacc tcttgttttt atgctaaaat tgggtgagtt
     5821 aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt tgctgaaaaa agtctgctca
     5881 aaccttggga tgtttttatt attttgcttt tgactgttct ttctttttta ccagtcatga
     5941 tttttggtct tcagcaaaac accgcaacag cagaaaaaga agcggtactg cgtgtggatg
     6001 gcacagaaat caaaacattc cctttgatgg ctggagaaga gagcaacaca tatacttaca
     6061 aagatgaaca cggagattat aacttgatcg agatagatgg cgatcgagta cggataaaag
     6121 aagcagattg cgatgaccag atctgcgtac gtcgaggctg ggcaacgaaa aatggcgaaa
     6181 ccatcgtctg tttgccacat aaattagtga tcgaagtccg ttcaacagat ggaggtgacg
     6241 aagacagtct gatttattag accttcacac accatgagta aattacaaaa aatgatttat
     6301 atttcgctac ttgttgcaca aggagtgatc attggtttat tggaaaatat gatcccttat
     6361 cctttttctt ttgcgcctgg tgcaaaacta ggcttagcca atctaatcac gatcgttgcc
     6421 atttttacca tgaaaaaaca agacagcttc ttactacttt ggctacgact attcctgact
     6481 actttgctag gcggaacgat ttccacattc ctttatagta tgagtggtgc tttgcttagt
     6541 tattttggaa tgttattggt caagcaatta ggaccaaaac gtgtcagtat catcggaatt
     6601 agtgcggccg gcggtttcat gcataatgtc ggacagcttt tgatggcttc tttcattgca
     6661 caatcatgga cagtcatgtt gtacttgcca gtcctttcag ttttagggat tttatcaggg
     6721 attgcaatcg ggatcgcagc gaattatctt ttacgacatg tgcagacact tcaacgattc
     6781 caaaaagatt atgagcaggg aaccaaacaa aaatggagcc caatgtttac caaaaaataa
     6841 tcgtcacaca aggagttttt ttatgaagct acatccaatg tgggaaaact atcctgcttt
     6901 aagtaaagaa ttgacagaaa cactaaaacg gatggaagaa actgtccgcc tgaaaaacaa
     6961 acctgtcgaa caagccatca aagaaacgat ccatgccggc ggaaagctgt tgcgtcctgc
     7021 ctatcagcta ttgttctcac aatttggacc agagcaagat cgagacaaag ccatcgcttt
     7081 ggcagcttct atcgaaatgc tccatacggc cacactgatc catgatgata tcgttgatga
     7141 agcggatctg cgacgtggtc agcctaatat ccgtagtcag tttggtaatg tcgtttctgt
     7201 ctatgcagga gactatttat ttgtctgctg ttttaaacta ttgtctgatt actccacttc
     7261 cttgaaaagt ctccaattga attctcgcag tatggaaaaa gtattgaatg gcgaattagg
     7321 gcagatggat gatcgctata attatgaact ttctgtaaaa gaatatttgg aaaatatttc
     7381 aggtaagaca gctgaactgt tccaattaag ttgttctgtt ggcgcttttg aaagtggaac
     7441 aagcgagcgc ttcgcaaaaa aagcaggaga tatcggactt tctatcggga tggcctttca
     7501 gattatcgat gatattttag attatacaaa agagagccag gaaatcggca aacctgtact
     7561 agaagacatg cgtcaagggg tctattcttt accgttgatc tactctttgc aaaaaaacaa
     7621 accaaagcta ctaccttatt tagagaagaa agcggcaatg acagaagaag atgtcgacgc
     7681 cgtacgcaaa atcgttgaac atacaggagg cgtggaagaa gctaggaagt tagcggcctc
     7741 ttacacagaa aaggctttga aggaaataaa gaaattacct gccacctctc ttcgaacaaa
     7801 agagaatctc ttttccctta cccaactgat tttggatcgc aaagactgaa tagggaaagg
     7861 ttcgatacgt aaataaattt ctcagaagga aatctacgtg ttttttgaca aattgaattg
     7921 tagttcttgt atttatgata aaaagcaaaa ataaagagca acgtccgtta gcgacgttgc
     7981 tcttttttat cttgaatatt tttttacctt ttcgatttct tataactcat aaaagtgatt
     8041 cttaaaccga ttacaagtaa tataacgcct gcgagtgtta tccaattatt cgattgactt
     8101 ccagttttag gaagtgctct ttttgatttg tttccattgt cattgcttgt tttattagtt
     8161 aagttaaagt tcgtcctgtt atccgaactt gtttttgaga aggagacact tgcatgacgt
     8221 gtatcgcttg tggtagacga cgtgtctgta ctatcgcttg aagttgatga atctgagcta
     8281 tcacttgtgg tagagga
//
LOCUS       DACCQI010000114         8235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCQI010000114 DACCQI010000000
VERSION     DACCQI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.118"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(451..1233)
                     /locus_tag="IVO76_002329"
     CDS             complement(451..1233)
                     /locus_tag="IVO76_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430266.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="IVO76_002330"
     CDS             complement(1298..2134)
                     /locus_tag="IVO76_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7430267.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="IVO76_002331"
     CDS             complement(2152..3561)
                     /locus_tag="IVO76_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7430268.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="IVO76_002332"
     CDS             complement(3575..5398)
                     /locus_tag="IVO76_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7430269.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="IVO76_002333"
     CDS             complement(6241..6567)
                     /locus_tag="IVO76_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430270.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="IVO76_002334"
     CDS             complement(6613..6975)
                     /locus_tag="IVO76_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP7430271.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="IVO76_002335"
     CDS             complement(7012..7227)
                     /locus_tag="IVO76_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430272.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="IVO76_002336"
     CDS             7354..7689
                     /locus_tag="IVO76_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430273.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..>8235)
                     /locus_tag="IVO76_002337"
     CDS             complement(7765..>8235)
                     /locus_tag="IVO76_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430274.1"
                     /translation="KICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGT
                     YLMTEKELINTVCERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKAS
                     SSFLIYLAKGAGIDETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
BASE COUNT         2543 a         1743 c         1270 g         2679 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatacaaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atctt
//
LOCUS       DACCQI010000115         8228 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCQI010000115 DACCQI010000000
VERSION     DACCQI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.119"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IVO76_002338"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IVO76_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7430275.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IVO76_002339"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IVO76_002339"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7430276.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IVO76_002340"
     CDS             2591..3376
                     /locus_tag="IVO76_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7430277.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IVO76_002341"
     CDS             3389..4240
                     /locus_tag="IVO76_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7430278.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IVO76_002342"
     CDS             complement(4364..4816)
                     /locus_tag="IVO76_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7430279.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IVO76_002343"
     CDS             complement(5281..5649)
                     /locus_tag="IVO76_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7430280.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IVO76_002344"
     CDS             5799..6281
                     /locus_tag="IVO76_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7430281.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IVO76_002345"
     CDS             6318..7328
                     /locus_tag="IVO76_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7430282.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IVO76_002346"
     CDS             complement(7443..8156)
                     /locus_tag="IVO76_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430283.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACCQI010000116         7979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCQI010000116 DACCQI010000000
VERSION     DACCQI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.120"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IVO76_002347"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IVO76_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7430284.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="IVO76_002348"
     CDS             1228..2331
                     /locus_tag="IVO76_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7430285.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IVO76_002349"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IVO76_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7430286.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3821..6268)
                     /locus_tag="IVO76_002350"
     CDS             complement(3821..6268)
                     /locus_tag="IVO76_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7430287.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6457..7893)
                     /locus_tag="IVO76_002351"
     CDS             complement(6457..7893)
                     /locus_tag="IVO76_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7430288.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2215 a         1736 c         1358 g         2670 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt
     3781 ttattggtta ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg
     3841 gcaacgtttc tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg
     3901 acgggccaac aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac
     3961 cgatccgaaa tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg
     4021 cttttgcttc ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt
     4081 catctgtcgg catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa
     4141 cgacccgttc tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct
     4201 cacccattgg caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat
     4261 caatgatcgt ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat
     4321 ttgctttgat gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat
     4381 gaatccccgc tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc
     4441 tggctaccat catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg
     4501 gatgtttcgt tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat
     4561 ttcttactcc ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt
     4621 gtaaggcttg cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc
     4681 cgttgtacat attcaattca accattttcg gatcgatcat catcagtttt acttcatggg
     4741 gcttagcacg cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac
     4801 cagtcgaacc agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc
     4861 cagaaacatc acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt
     4921 cgatgatttc tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca
     4981 gtgatttacc tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga
     5041 tatcatctgt taagttcaca attttactta cttttacccc tacagccggc tggacttcga
     5101 attttgtgac ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt
     5161 ggaaagtttg ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat
     5221 ccacagttgg tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct
     5281 gttctgcttc aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa
     5341 aaccttctgc tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag
     5401 gatggatatt ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat
     5461 tttctggttc ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct
     5521 tctcatattc tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt
     5581 tcgcttctgc gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg
     5641 caggatcacc ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca
     5701 gctgatagcc ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg
     5761 ctaaataact tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa
     5821 tcatcccacc accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat
     5881 cccatgtcgt ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat
     5941 gtaacaataa taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac
     6001 caattgaaaa ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac
     6061 agaggatttg gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta
     6121 aaaaacctaa ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa
     6181 tcgtcagcat ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt
     6241 ttttagtagg ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat
     6301 acaaaaaaaa gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt
     6361 tcagcttatt ttacagtttt tcttaatttc taaatgattt tatcaagaaa aggcacggcc
     6421 atatccagcc gtaccttctt gttttaagta tgtgaactaa aggttctctc cattttcttc
     6481 aatcagtttt tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg
     6541 tgtttcgtct tgatcaacat atacaaatcc atatcgcttt tgataaccgt tcagccagct
     6601 aagcaaatct gtataagacc aggtgcaata gcctaggact tctgctccat ccgaaattgc
     6661 ttctttgatg gctttgacgt ggctttgtaa gtaattgatt cgatagtcgt catggatttg
     6721 tttatcttct gtcaacttgt catactctcc taagccattt tccgtaatca gtaccggcat
     6781 gcggtaacgg cttgtgatac gacgcagtcc gatccttaat ccttctgggt caatctccca
     6841 gtcccaattt gttcgttcga caaatggatt ctcaactttt ttatacattc caggaactcc
     6901 agtttcttct gatgaacctt tttttcctgt cgtattcatt tttccagcac caactccgtt
     6961 taatggattg aatgcattcg tcgccgtttg atagtaattg atcccaagaa aatctggttt
     7021 agcagattct agcaattctg catcgccagc ttcaatcgtt ggtgcccatc ctttttcttc
     7081 taagaacctc atggcagcaa tcggatattt tccccacata tagacatcca tccaataatt
     7141 ggccatcagg tcttctgcat tttcagcagc cagaacattt tcaggattac tgtcaattgg
     7201 ataatttggc gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcgtgaaa
     7261 tttattgatg acagatgcat ttgctaaatt agcaatatgg ttaacttcat acattcgttt
     7321 cgggtctgtc acacctggcg gatgtgctgc taacaaataa cctaatgaag tgaagatatt
     7381 ttgttcattc aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc
     7441 tgcataatga gtgaaatctt caatgatctg acgtgattcc caaccattat attcatcttg
     7501 caatgcttgt ggaagatccc aatgatataa agtcaacata ggttcaatat cgttttcaat
     7561 caattcgtcg atcaatctct cataaaaagc gagcccagct tgattgactt ctcctcgtcc
     7621 attaggaaaa atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt
     7681 cattaaagca atatcttctt tataacgatg atagtggtct actgccaaat caccgttcgt
     7741 tcctttaaat gtctttccag gtatccgtac aaatcgatcc cagactgatt ctcctttccc
     7801 atcttcttgc caagctcctt ctacttgata cgctgcagaa gcagagcccc acagaaaatc
     7861 ttttggaaat gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca
     7921 atcgttcctc ttttagaata acacgaaacc ccttccacaa caaaataaaa atatgtaaa
//
LOCUS       DACCQI010000117         7933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCQI010000117 DACCQI010000000
VERSION     DACCQI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.121"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            372..1745
                     /gene="glmU"
                     /locus_tag="IVO76_002352"
     CDS             372..1745
                     /gene="glmU"
                     /locus_tag="IVO76_002352"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7430289.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            1839..2810
                     /locus_tag="IVO76_002353"
     CDS             1839..2810
                     /locus_tag="IVO76_002353"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7430290.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      2816..2918
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2984..3553
                     /gene="thiT"
                     /locus_tag="IVO76_002354"
     CDS             2984..3553
                     /gene="thiT"
                     /locus_tag="IVO76_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7430291.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(3634..4407)
                     /locus_tag="IVO76_002355"
     CDS             complement(3634..4407)
                     /locus_tag="IVO76_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430292.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            4674..6374
                     /locus_tag="IVO76_002356"
     CDS             4674..6374
                     /locus_tag="IVO76_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP7430293.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            6344..6562
                     /locus_tag="IVO76_002357"
     CDS             6344..6562
                     /locus_tag="IVO76_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430294.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            6582..7472
                     /locus_tag="IVO76_002358"
     CDS             6582..7472
                     /locus_tag="IVO76_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7430295.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            7490..7774
                     /locus_tag="IVO76_002359"
     CDS             7490..7774
                     /locus_tag="IVO76_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430296.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         2690 a         1364 c         1664 g         2215 t
ORIGIN      
        1 aaatcagcgt cctgtcctga gtattagctc aataaacgga aaaattgctc ctcatatttt
       61 gccgtttatt gagctaatac cgaaggtcag ctgattgaaa aacgtttaat gaagaggttg
      121 taagcctacc gtttaactct aagtcacaag aaacgatttc aaaaaacagc ttttcatgtt
      181 ttatgagtag tgtgacttgt gaccgaggag ttggcaggag agcaccggtt accaaataga
      241 taggcccgtt atggtctatc tattttttta tccagaaaac gctgggataa aagaaaatca
      301 cttgttttgt tgaaaaaatg ggtatactgg gataaactga aatcagcaaa ctatatttag
      361 gagtgacgca gttggacgca cgttatgcaa ttattttggc ggcagggaaa ggtacccgca
      421 tgaagtcaaa attatataaa gttttacatc ccgtttctgg taaacctatg gtagaacata
      481 tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat tacgatcgtt ggacacggag
      541 cagaacaagt taaagcacaa ctaggagaac gcagtaaata tgcgttacaa gcagaacaat
      601 tgggaacagg acatgctgtt ttgcaagctg cttctttctt ggaaggaaaa aaaggaacaa
      661 cgttagttat cagtggcgat acaccattat tgactactga aacgctgaac aatctttttg
      721 aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac ggcacaagca gaaaacccaa
      781 caggatatgg acgaatcatc cgtgaccata ttgggatcgt agaaaaaatc gtggaacaaa
      841 aagatgcaac accagaagaa gctcttgtac aagaaatcaa tacaggaact tactgttttg
      901 acaacgaagc tttgttcgat gcgttaagca aagttggaac aaacaatgct caaggtgaat
      961 actatctaac agatatcatc gaaatcttaa aagaagaagg ccatacagtt gctgcttatc
     1021 aaacggatga ttttgaagaa tcaatgggag tcaatgatcg gatcgcgtta gctaaagcaa
     1081 atgagatcat gagaaagcga attaaccaga tgcatatggt caatggcgta agctttgtcg
     1141 attcagcaac gacatacatc gatgcaggcg tagaaatcgg tccagacaca ttgatcgaag
     1201 caggtgtaca aatccaagga aatacagtca ttggttctga ttgtgtcatc gggtcacatt
     1261 caaaaatcgt agacagtagg attgaggatc acgtcgtcat tgaaaactca gtcattgaaa
     1321 gcagccatgt caagaaacat gcagatgtgg gaccttatgc acatttacga ccaaaagctg
     1381 aaattggcga aaacgtccat atcggaaact ttgtggaagt gaaaaatgct caaatcggca
     1441 aaggaactaa agtcggtcac ttgacttatg ttggtgatgc tacgctggga gaagaaatca
     1501 atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa gaacaaacat catacgactg
     1561 tcggcgacca cagctttatt ggttctagca cgaatatcat cggacctgtc gaggtagcta
     1621 aaaacagttc gattgctgcc gggtcaacaa ttacagacaa tattccagaa tatgcgctgg
     1681 caattgcgag agcaagacaa gtcaataaag aaggatatgc aaaaaaactc ccttatctaa
     1741 actaagactt ttgcaaaatc acttgattta tttaaaaaat atgactacta tttattaggt
     1801 agaattctag agttgtgaaa aagaaagtgg aggtccccat gtcaaaacat tactttgacc
     1861 caagattaaa aatttttgcg ttaaattcca atcgcccatt agcagagaaa attgcagcag
     1921 cagtcggcgt cgagttagga aaatcatctg ttactcaatt tagtgatgga gaaattcaag
     1981 taaacattga agaaagtatt cgtggatcgc atgtctatgt tattcaatca acgagcagcc
     2041 cagtcaatga taacttgatg gagttgctga tcatgattga cgcactgaaa cgagcaagtg
     2101 caaaaacaat caacgtagtc atgccttatt atggctatgc gcgtcaagac cgtaaagcac
     2161 gtgcacggga accaatcaca gcgaaattag tagcaaacat gattcaaaaa gctggtgcaa
     2221 caagaatgct gactttggac cttcatgcag ttcaaatcca aggtttcttt gatattccag
     2281 tcgatcattt gatgggtgcg ccacttattg ccaattattt cttagaaaaa ggaatcaaag
     2341 gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt cacacgggca cgtaaattag
     2401 cggaattttt gaaatctccg attgcgatca tagacaaacg ccgtccaaaa gcaaatgttg
     2461 cagaagtaat gaatatcatt ggacaagtca aaggcaaaac ttgtgtactg atcgatgata
     2521 tgatcgatac agcaggtacg atcacattgg ctgccaatgc tttgaaagaa gctggtgcaa
     2581 ctagtgtgta tgcttcatgt acacatccag tattatccgg ccctgcttta caaagaatag
     2641 aagattcagc tatcgagcgt ctagttgtta cagactcgat ctatcttcca gaagatcgta
     2701 aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat gggagatgca atcaaacgta
     2761 tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa aaataaataa taaataaact
     2821 cacaaagggg agtccaattg ggctgagatt gaatctattt ctaaaccctt cgtacctgta
     2881 tcggttatgc gagcgtagga attgtgaata aacagctgtt tgactatttt cgaaagctgc
     2941 ttcctccttt gtgacttttt tattcaaagg aggaagtttt tttatgggaa aaaatagagt
     3001 ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg ttgtcgttga ttccgatcca
     3061 gatcggcagc agtttttcta tttcattagg gcagattcca ttgactcttt ttgcgttaag
     3121 aagagggaca aaggcaggga tgctggcagg tttcatttgg ggcatcctac attttccgtt
     3181 gggacaagtt tattatttaa gtgtcgttca agtgttgatt gagtacccga ttgcctatac
     3241 ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa aaaggctatg tagacgataa
     3301 tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg attggcgcac tttccagata
     3361 tttttggcat tttattgcag gagtggtttt ctggggagcg tatgcactat gggggatgaa
     3421 tccttggctc ttttctttcg taatgaatgg tgccagcggg ttgagtacag ctattgtaac
     3481 gatcatcgtt ttattaggga tccaacaaac gactccttcg atttttgtgc cagataaacg
     3541 acaggagctg taaataaaaa agaagcaaga aacttgagac agaatcatgt cctagtttct
     3601 tgtttccttt ttattttttg ataagaaata gatctacttc aatggcagat aagaaagatc
     3661 ggcatcttct accaattgcc agtgtcgatc ggtgacagag tagattaaag tagcgatgat
     3721 ttcctcttct cgataccggg tgccttttaa tttccaaata taatcttcaa tcacatagat
     3781 tgggatacga tatttaccat cgatcaaacc aacttgatca aaagaaaaaa tgatcccttc
     3841 ctgcatcaaa ttacgcattt ctttcaataa ttccacactg attttttcaa caggtgtttc
     3901 ttttttgaaa aaagattttt ttacattgat cttatctgac aagatcgaac ttaatttact
     3961 agcaaaaatc ctataaaatt catctaaata gcgataataa atccgattca gctgatcatt
     4021 aagatcgaag tatacaaaat tattctgtag tttataaaaa aacggcgaat gaagatgtgt
     4081 tttcatatga ccaaaatata ataattctga aatttctaaa ggtgtcaatt ctttcagcat
     4141 cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat ccacgcctgt tacgagaaga
     4201 ggaaaagaaa cgctgaatat tttctgtacc acggattact ttcagccctg tatgggattc
     4261 atattctcca gcagaagcag atggatcaag caagagcaag ttttgaggaa aatgcacgat
     4321 actgtttgca aagtcagtag cagtcagccc ttttgttaaa acagcattac ttgtggtatc
     4381 aatatgtaca taaactgact cagtcatcat gttcctcctc ttatttcctc ttaatctctt
     4441 ttattttaca acatttgcat taaaaaagct tgtttctttt attttaaaat tccattacaa
     4501 aatcacgttt tcattgaaaa cactctcatt ttcaattaat ttttgtgatt tttgtatttt
     4561 tttatttaaa ggaggtttta tgaattgttt tcttatggaa tctacaaagt cgaatacgag
     4621 actcatgacc tttttatgac gtactggtat acttgaagaa aaaggagagc gcgatggaga
     4681 taaaaaaagt ttacttttca cagaaaatgt cgctgtattc gcctatccag caagcaaaac
     4741 aaccccttcc attttttcaa agtcatcaag aaagtagaga cgctttttgg cgttctgcaa
     4801 aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac cgtctatttt actattgcta
     4861 aaagtagagg agatgttatt tttaataatc tgttgattga aggtgaaact tttgaatgga
     4921 aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt tattaaagaa aactgttgtt
     4981 ttttatttca atctcctcta tcagaagaat ggttgcgtat ttttcataaa aacggctatc
     5041 aaggaaccac acaattaaat aaaaagctgg tctatcatac agcacttgtt ttaggtggag
     5101 gaggagcgca cggtgcttat cagattggtg tgtggcaggc gctgaaggaa catgatatca
     5161 actttgagat tatcacaggt acctcggtag gtgcgttgaa cggcgcattg attttacaag
     5221 gagatatgaa aaaagctgtg aatttatgga aaaagctgtc gacaagacaa gtgcttgcgt
     5281 taccagaaat ggctgcagtt gaagaccttc gtgaacgctt ctatcgagaa agacgccaaa
     5341 tgacgaaaac agcactaatt gaaggcgggg tttcttctgc tccattggaa aagatggtca
     5401 aagacaattt ggatttgtcc ttactcaaac ataatcccaa aatacgttta tatacggtgt
     5461 ctactaaact gccagaacta gcggaagtag tgactgatat ccaacagaca aaaacagaag
     5521 aaataccaga ttggatactc gcctctgctt cattttatcc cgctatggct tatcgaaaga
     5581 tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa aatacctatt gatattgcga
     5641 ttaacaaagg agcaacagag gcatttgtcg tagatgtgca agggccaggc ccagcgaaaa
     5701 aaattcgtat gccagacatt tttatccatt ggaaatgtca aacattgtgg acactaggca
     5761 gctttctgct ttttgatagc caaaggaacc aattaaattt acaattaggc tatctggaaa
     5821 tgaaaaaacg attaggctat tactatggga actggtatac atttcattct gttaaacctg
     5881 caagaacata ctggcgagga tttttgtctt acttaacaaa agaaattcag ttagaattat
     5941 cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa tttatataag aacagggtcg
     6001 taccagagac atgcggattg gcgatgctag aacttttagc aaaaaaacat tttttactgc
     6061 ccaatgaaat ctatcaagta gacatgatga ttcaaatgat ctgccaacaa gatataaaaa
     6121 gtatgccgga tgatttattg gcacaaattg gtcaattaag tactgaagaa tggcgaaggt
     6181 atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa aactgtttat tttgattatc
     6241 tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt gcttaagcaa ccagttgaca
     6301 cattactgat cctatattta tattatctca aggaggaaca accatggcac aagaattttc
     6361 atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac aacaaggggt ggcgtaaaga
     6421 actgaaccta gtcagctgga atggtcgccc gccaaaattc gatcttcgag attgggcgcc
     6481 agaccatgaa aaaatgggga aaggcctgac attgacgaac gaagagtttg aacaactgca
     6541 aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg aatgaaaagt atgactggtt
     6601 ttggcaaagc catccgcgaa acaaaagcgt atcagttaga tattgaaata aaaagtgtca
     6661 accagcgttt tctagatgtt caaatacgaa gtcctaaaat cctgaacttt gttgaaaacg
     6721 attttcgtca gcttattaaa cagcagttaa gccgcggtcg aatcgaagta tttgtgaatc
     6781 tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa ctgggaattg attgattcac
     6841 ttatcactca attgacagac ggaattcaac aacgatacgg tagtcaaaat caagtgtcaa
     6901 ttagtcggat ggcggaacgc ttcgcgctgg acgaaaactt cgtaacggta gaagaaaaac
     6961 cggaagagag catggaagaa ttggcacagt tagctctaga tacattgaaa agcgcacttg
     7021 attctattga ggcgagtcga gaaaaagaag gtcaagcttt agctttagtg ttgaaaaaga
     7081 atactgaaga attcaaagaa gtactaaaaa aactcgatag ttttgtagaa atctatgaaa
     7141 aggaatttca aataaggtat caaaaaaagc tggaggaatt tcttggagcg aaagtcgatg
     7201 agcaacggtt gttgacggag ctagccatct tgttggaacg cggagatatt cacgaggaac
     7261 tggatcgtct ggcgatccat gtacaaaaac tagatgaact tatcgaagca caatgtcctg
     7321 taggtcggga attagatttc ctgatccaag aaatgaatcg ggaaatcaac acgatcggtt
     7381 ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca gtctaagacg atcttagaaa
     7441 aaatcagaga acaagtacaa aatattgaat gacgaaagag gtagagataa tgaaatatta
     7501 tgacgtaacg ttccacgaat taagtggaaa aactgtggtt aaacgggata tcccttctga
     7561 aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac tataatgaga acgaactgtt
     7621 tattttgatc aacgacggtg cttacgtgac gatgaatcgg aaatttattg ttcgaattga
     7681 cacagaagag gtagaagatc cgacagaaaa agcacgttct cgaaaagatg agatcatggg
     7741 tgtagtgaat actttgtcca atatgggctt ttagcaagga tgctgaacaa agaaatacaa
     7801 taaataatca gaattggatt tcttcgctta gcttctttgc tctaggaata catttaaagg
     7861 ttgtgacaaa attcgtgtca caatcttttt ttattttgaa taagtgatgt tcaaaaacca
     7921 ttttctcggt att
//
LOCUS       DACCQI010000118         7884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.122, whole genome shotgun sequence.
ACCESSION   DACCQI010000118 DACCQI010000000
VERSION     DACCQI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.122"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..968
                     /locus_tag="IVO76_002360"
     CDS             <1..968
                     /locus_tag="IVO76_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP7430297.1"
                     /translation="QIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIAD
                     LIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAIYMDATYIPLKRKTVAKE
                     AIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERGLKNVLLFITDGLKGMVG
                     AISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKEVALEARGTFA
                     EKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQ
                     NEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQLIEN"
     gene            1102..3291
                     /locus_tag="IVO76_002361"
     CDS             1102..3291
                     /locus_tag="IVO76_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7430298.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3288..5081
                     /locus_tag="IVO76_002362"
     CDS             3288..5081
                     /locus_tag="IVO76_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7430299.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            5281..5595
                     /locus_tag="IVO76_002363"
     CDS             5281..5595
                     /locus_tag="IVO76_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7430300.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            5616..5993
                     /locus_tag="IVO76_002364"
     CDS             5616..5993
                     /locus_tag="IVO76_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7430301.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            6003..6776
                     /locus_tag="IVO76_002365"
     CDS             6003..6776
                     /locus_tag="IVO76_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430302.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            6798..>7884
                     /locus_tag="IVO76_002366"
     CDS             6798..>7884
                     /locus_tag="IVO76_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7430303.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLI"
BASE COUNT         2794 a         1244 c         1443 g         2403 t
ORIGIN      
        1 tccagattcc gcgcgaccgc aatggtgagt tcaagcaaca gactgttcct gcttatagac
       61 ggacgaatga cacgttagag gagaccgtca ttcacctctt ccgaaaaggt attaccatgt
      121 cggaaatcgc agacttgatt gagaaaatgt atgggcatta ctacacgccc caaaccatgt
      181 ccaatataac aaaatcattt acagaagagg taacggcgtt taatgggcgg gagcttcatg
      241 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      301 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      361 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttggaccttc
      421 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      481 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttccc
      541 gtaatatcag tcataaagtg cgtgtcgatg atcgtaagga agtctgtgat gattttaaaa
      601 tggtgtatca agcctcatct aaagaggtgg cgttggaagc acgtggtact tttgcggaaa
      661 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      721 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      781 cctttaataa acaaatcaag aaatacagcc accgcaagga acagttccaa aatgaagagt
      841 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      901 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctaagc caactgattg
      961 agaattagaa acaatctgca gtagggaaga atcatttaca caaaattatt gacgctccct
     1021 tataatgaga acttttacta acataattta tgataaactt actatataat catattaatt
     1081 aaatagttag gagatctgct tatgtttata tctactcttc aaattcgaaa ttttagaaat
     1141 tatgaatccg aaatctttcg attcgataat gagacaaata ctattatagg tgagaatgat
     1201 tcagggaaaa ccaatgctct tactgcattg agaatcttat tagatgatag ttattactat
     1261 agtagtaaaa cactaaaaga atcagatttt tttcatggaa ttcaaaatgg gtggcaaggg
     1321 cattggatta ttatctctgc gacatttgaa ggaatcagcg aagaagaatt tgacaatgag
     1381 atttgtgctt ccatttcttt aaattctgaa agtcaaacaa tcttagaaga attgatttca
     1441 aatgcagata aaggtgtagg tagtatttct ctatttatta gacctaacaa agctattaga
     1501 aagcaattat ttgatatttc cactgaatta gactctcatc aatttaacga atttagaaat
     1561 tctattcgtc tatccgacta tgaattttac tacacatcta aatcaaatct aaatttctgt
     1621 atcaatgaaa attatgataa attggtagga agactcaatg acaatatagc tagtaatcct
     1681 gaagaagacg atgaagctct gttgggctca agaattgaca tgtcagatat tttcaagcat
     1741 atttcggttg tttatgttga cgctcttcga gatgttctta gagaaatgaa aaacaatcga
     1801 aacccagtca aaagaatcat ggaaacaatt gaatcaaaaa tatcatcaga taatgttgag
     1861 tcattaaaaa caattataca gcaattaaat gagactatta ccagtgttcc tgaaattagg
     1921 aaaattggcg aaaatattaa tcggcaacta aatagcataa ttggttcagt gtattctcct
     1981 aatcttcttc tttcctcaac tatgtcagat gatatgggtt ctttagcaaa atttctttca
     2041 atgaagccag aacaaaatat cgacttagac ttactagggt taggacatct aaatatgatt
     2101 tatttagctt tgaaaatagt cgaatttgaa gcgtgtcgtt caagagaact acttaatatt
     2161 atgttgattg aagaaccaga agctcatatt catcatcata ttcaaaaaac tctgttcgaa
     2221 ggattaaatc ttcaaaaaaa ctatactcag attctcatga ccacacattc tgtacattta
     2281 gctgaagtgt ctgaaatatc tcggatgaat gtattaaaat cgtttaatgg caaatctatc
     2341 gttatgaatc cagcaaataa tctgaactca tttgctaaag aaaagttaaa aaagaacaat
     2401 ctaaatctaa taaacgcagt agagagatat ttagatgtta aaagaaatgg attgcttttt
     2461 tctaaaggtg ttatcttagt tgaaggagat gctgaagaaa tacttattcc tcaaatagta
     2521 aaaaaattat ttggtataaa cttagacgaa cttggaatag gtttgattaa tgttggtagt
     2581 acttcgtttg aatatatcgc tagcttgttt gacgatgata gaatacagag aaagtgtgct
     2641 attgtaactg atttagatga acaggcaatc ccatcagaac atcgattgta taaaagcact
     2701 gcacaagaga aaggtcaagc aagaaaagaa aaattatcct ccctttttaa tgaaaacaat
     2761 tgggttaatt gtttttatgc taatacaaca tttgaaattg agtttttaga tgtgaataaa
     2821 gaaaatattc taacttatgt cagtccaatt atagatgacg cattcaaaga taaaaaaaca
     2881 ataactgctt ataaagaaga aatacaaagt gaacaatatt tagaaagaaa tttagcgatg
     2941 ttaaaactag ccaatcatgt tggtaagggg tggtttgcaa ttgagttagg tgaaaaaatt
     3001 gactttttaa tcaaaattcc taagtatatt cttaatgcta ttgcttttgc atgtcaggaa
     3061 atagtagatc tctcaattta taaaaaaatt ctactccata caatcgactt gtacgaggaa
     3121 actacagaat tgtcagcttt atcagctagt ttaaaaacaa gtgatacagc tgatttgaat
     3181 atagcaataa tttctttatt agagtacttt ggtaaagatg aggatgttgt gtatctaatc
     3241 aatcagatag aaaattacac aaaaatactt tatgctgagg tttcaaaatg acaaatatct
     3301 tcgaaaaaat cgtaaatgat ttaaatttag aacaaaaaga tgttgtggaa agtgaagata
     3361 acctttatgt aaatgcatgt cctgggagtg ggaaaacaag ggtacttaca aggaaaattg
     3421 cttaccaagc aattaaacac gatggatcac taaaaagaat aattgctatc acatacacta
     3481 atagagctgc tgaagaaatt aaggaacgcc taagtttact tagtatagat gatgatatta
     3541 atatttgggt ggggacaata catcagtttt gtcttgaatt tattatttat ccttttaaaa
     3601 tgaatctctc aagagtatca aaaggattta caattattga tgattatact caaagagctt
     3661 acgtcagcca aattaataat atgctgaatt taaatctcaa aacgtatgaa ctgaacaaaa
     3721 ttgatttgtc actttctcat gattttaaaa ttaatgaagt tcagtatact caagtttctg
     3781 aaatgtatca cactatgttg gaaaaaaata aagaaattga ttttgatatg attttactag
     3841 tagcgtataa aattttaact gtaaacagta acgttgcacg taatattgct agtaatataa
     3901 gggcgattta tgtggatgaa tttcaagata ctagagaatt acaatacaat gtaatagcta
     3961 aattacttca aaacaaccct caaattcagt caatgtttgt tggagatatt gaccaagcaa
     4021 tttatggatc attagatggt atagctaaat cagttgaaga actagaaatg ctaactagtc
     4081 acacttttca atcaaagacg ctacatggct gttacaggtc taatcagaga ttagtagact
     4141 tctactctaa ttttcagagt tgtccttacg agattgaaag cagagggaat aataaaaacg
     4201 accgtggttt tataagttat gattgcaaag ttactaagga ggatttaccc aacataataa
     4261 aaaatataat aaaggaaaag attgaaagcg gcattcatga aaaagatatt tgtgtacttg
     4321 ctcctcaaca ctatcctctg ttttcactgg gtgagtattt aaagaaagaa ttaccgtatt
     4381 gcaattttga tgctataaat atatcaccaa ttaaggtaaa taatcatagt ttatttttca
     4441 agttagcaat tttgtacttt actgaatcag gtgaaaaggt tcgaagaaga aagctaattg
     4501 caaacgatat tttaattata ttaaaaaatg aatttggaat agaagttaaa gattatttta
     4561 ttgacttaga tttattacag ctaataaaca gtgtgaaaag aatgtatagt ggagacgaca
     4621 acggtgttga tttatacaaa tttgttacca aacatatatt tgttaatctt aatattgacg
     4681 agaaaaatta cccgtctatt tttaagcact atctattttt tattaacgaa gtagaagatc
     4741 ggataaaaaa taattcatta tctagtagtg tatctagttt taagaagatg tttaaatctc
     4801 gagatggtat cacacttact acagcacata aagttaaagg aaatgagtat gatactgtta
     4861 tcgctattaa tcttctaaac gggaaaattc ctcattggaa tgaaattttt aatgtttctg
     4921 ataaaggaat tagttcagcg agaaaactac tatacgtaat ttgttcaaga gcaaaaaata
     4981 atttatttct tttctcagag gtgggttatt ttacgagtaa aggagacttg ctttatccca
     5041 ctcaattatt agataaaata gaatttgatt ataatattta aaatacctag gtagtcgttg
     5101 ttttaaatat agtaggaatc ggacgctcag tacggagcgt ctattttttt acccaatttt
     5161 gaaaggaagt gaacttatga aaataaaaaa tcaagaatca aaaggtcgct ctcccccctt
     5221 taaaaccatc acacaaataa gattcattca aacataaaaa cagaaaggat aggtaaaaat
     5281 atggaactta aatatgtcat tcccaatatg gaaaaaacat ttggacaatt agaatatgct
     5341 ggtgaaggga acattgaaca acgtcgaata aatggcagac caaccattct ctctcgtagt
     5401 tacaatttat actcaacgat tcaacgagca gatgatattg ttgtcattct tccctccgaa
     5461 gctggcgaga aacattttga atccgacgaa aaagtcaaac tcatcaaccc acatattacc
     5521 gcagaaggtt ataaaattgg caatcgagga tttaccaatt acattatgca cgcagacgat
     5581 atggtgaaag cataagaaag gaaaggtgaa aaaatatgag attagcagaa ggtattgtga
     5641 ttgaaaaaga ccagacattt ggcacattga aattttccgc cttgcgtcgt gaggttcgta
     5701 tccagaatga agatggtacg gtttccgaag aaattaaaga acgtacctat gacttaaaat
     5761 caaaaggaca aggacgcatg atacaagtca gtatccccgc ttctgttcct ttgaaagaat
     5821 ttgactacaa cgcagaagtg gaattaatca atcctgtggc agataccgta gcgacggcta
     5881 cttttcgagg ggcagacgtt gattggtacg tgaaagcaga cgatcttgta ttaaaaggac
     5941 agacaaaacc tcatcagaaa tataaaaaag aagcgtccga aagtgaaaaa taggtgttgc
     6001 ttatgacaaa cacaccattg attttgaaag aaattccgaa ggatgaagcc atttctttta
     6061 ttcggcaata ccattattca aagatactcc ctagattgtg taaatacttt ctagggattt
     6121 tttcagaaga aaaattatta ggcgttgtgg aattaggctg gggtacgcaa cccctacaaa
     6181 ccatacgcaa actctttccc gacagttccc ttcaaaccac agattattta gaaattggaa
     6241 agatgtgttt tcttccagaa atgaatcaaa ccaattattt tggcagtcaa gctctttcag
     6301 ctctaattaa gtggttgaaa gaacatacgg actgtcattt tttatataca ctggcagatg
     6361 gaattgaggg aaaatgtggc tatgtctatc aagcgtccaa cttcttttac tgcgggtatt
     6421 tcaagacaag tgtctatcgt gataagcagt catgggaaaa gatacaccct cgaagtgctc
     6481 gccttttatt agaggaaaat gctcgctttg aacaggtaga gaaaaagcat tggctttccc
     6541 aggcattttg cgaatacaaa gggattgaga aaatcaatgg acgcatgttt cgctatctct
     6601 atccgttgac aaaagaagcg aaaaaactgt taggacacac tctctatcga cgtcattatt
     6661 atccgaaaga aaaaaattta cgctttgaaa agcgaatcgc ttatcagaaa tatgaagcca
     6721 tttctcaacc aacttttgat aaacaggctc gtatctacaa tacacaatta ttttgatagg
     6781 tggaaaggag gatttttatg ccattgcttc atgacagagg agagcgaatc aaagccaacg
     6841 ataagcactt ggtctatcat tttgtactaa tttcattgct ggtagttttt ctagtcgtcc
     6901 tactcccttt tcattggaga gcgttacaat cggtgaactg gcaagcagtt cagttagata
     6961 catttttcta tcaacttcat acgccatact tttggagcag tattctcact gctctttttg
     7021 tatgcggact tatcagctgg ctttattatc actatcgtat agattctata aaacagttag
     7081 agcaccgtca aaagttagct cgtatgattt tagaaaatca ttggtatgaa gccgaagaaa
     7141 ctcaatcaga gggattcttt aaagatttac ccagcaagaa aaccaaacaa cgaatccgcc
     7201 attttcctaa aatatattat cgaatgaaag atggactaat ccatattcat gtagaaatca
     7261 ccttagggaa atatcaagat cagcttttgc acctagaaaa caaactagaa acaggattgt
     7321 attgtgaatt agtggaaaaa acgttgaaag attcttatgt agagtatgtc ctactttatg
     7381 atactattgg caatcgaatt ggcattgaag acgtggtagc tcaagatgga cgtgtcaaat
     7441 tgatggaatc ggtctattgg gagttcgatt ctctccctca catgttaatt gctgggggca
     7501 ctggtagtgg aaaatcctat tttattctca cattgattga atctctcctg catacaaatg
     7561 caaagcttta tattcttgac cctaaaaatg ctgatttggc agacttaggg gcggtcatga
     7621 ataatgtcta ttaccgtaaa gaagatatgt tggcatgtat caatcaattt tacgaagata
     7681 tgattgatcg cagtgaaacc atgaaacaac accctaacta taaaacagga gaaaattatg
     7741 cttatcttgg tttgtctgcc aattttctta tttttgatga atatgtggca atgatggata
     7801 tgctaggtcg tgaaagtacc accgttattc ataaactcaa acaaattgta atgttaggac
     7861 gacaagcagg cttcttttta attc
//
LOCUS       DACCQI010000119         7825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCQI010000119 DACCQI010000000
VERSION     DACCQI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.123"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IVO76_002367"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IVO76_002367"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7430304.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IVO76_002368"
     CDS             1007..1927
                     /locus_tag="IVO76_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7430305.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IVO76_002369"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IVO76_002369"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7430306.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IVO76_002370"
     CDS             3233..4711
                     /locus_tag="IVO76_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7430307.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="IVO76_002371"
     CDS             4988..7048
                     /locus_tag="IVO76_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430308.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="IVO76_002372"
     CDS             7131..7700
                     /locus_tag="IVO76_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7430309.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         2790 a         1308 c         1495 g         2232 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga agggg
//
LOCUS       DACCQI010000120         7761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCQI010000120 DACCQI010000000
VERSION     DACCQI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.124"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            147..1094
                     /locus_tag="IVO76_002373"
     CDS             147..1094
                     /locus_tag="IVO76_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7430310.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            1106..2026
                     /locus_tag="IVO76_002374"
     CDS             1106..2026
                     /locus_tag="IVO76_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7430311.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            2067..3524
                     /locus_tag="IVO76_002375"
     CDS             2067..3524
                     /locus_tag="IVO76_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7430312.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3869..4483
                     /locus_tag="IVO76_002376"
     CDS             3869..4483
                     /locus_tag="IVO76_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7430313.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4476..6200
                     /locus_tag="IVO76_002377"
     CDS             4476..6200
                     /locus_tag="IVO76_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7430314.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            6197..7648
                     /locus_tag="IVO76_002378"
     CDS             6197..7648
                     /locus_tag="IVO76_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7430315.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2618 a         1291 c         1543 g         2309 t
ORIGIN      
        1 ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg
       61 tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt
      121 gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta
      181 tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga
      241 tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt
      301 cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca
      361 aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg
      421 tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt
      481 tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat
      541 cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca
      601 atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt
      661 ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac agcattattt
      721 actttgcatc tgttatggga atcgatccga cttattatga agctgctatg gttgatggag
      781 ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga
      841 ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca
      901 ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca
      961 atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag
     1021 ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt
     1081 ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg
     1141 ctctttcaat aagacggcta attttttctc taatataatg attgcactct ttgctatttc
     1201 ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc
     1261 gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt
     1321 cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt
     1381 aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa
     1441 ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg
     1501 gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc
     1561 attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa
     1621 acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg
     1681 aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt
     1741 tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa
     1801 gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca
     1861 aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc
     1921 cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca
     1981 gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat
     2041 aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg
     2101 tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg
     2161 cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt
     2221 tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca
     2281 atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg
     2341 gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct
     2401 ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg
     2461 aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa
     2521 atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt
     2581 caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa
     2641 aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg
     2701 attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa
     2761 tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt
     2821 acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag
     2881 gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt
     2941 tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta
     3001 cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat
     3061 cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg
     3121 ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag
     3181 atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat
     3241 atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg
     3301 cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa
     3361 gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc
     3421 cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat
     3481 tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa
     3541 aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc
     3601 cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt
     3661 tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc
     3721 agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg
     3781 tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag
     3841 tataaactta atgtttggag gagaagatat gttagggaag gttttggaag agttatttat
     3901 ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg
     3961 gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg
     4021 attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt
     4081 aagaggcaat ctgctattta gtttattttt atctggtact gctttattga gttacaattt
     4141 atatttatct gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt
     4201 tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac
     4261 gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc
     4321 ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg
     4381 attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca
     4441 ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt
     4501 caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa
     4561 cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag
     4621 aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct
     4681 tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga
     4741 cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta
     4801 ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac
     4861 taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa
     4921 acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt
     4981 tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag
     5041 aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt
     5101 ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg
     5161 aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg
     5221 atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag
     5281 ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca
     5341 ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat
     5401 attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag
     5461 agacgcggat cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca
     5521 acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac
     5581 agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata
     5641 cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg
     5701 tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa
     5761 aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc
     5821 gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg
     5881 tgatccagcc attagttgaa aattatttta aacacggtat tgattttact cgattcgata
     5941 atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata
     6001 atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta
     6061 aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct
     6121 tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta
     6181 ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat
     6241 tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac
     6301 agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt
     6361 aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga
     6421 acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg
     6481 aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt
     6541 tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca
     6601 agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag
     6661 tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact
     6721 ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta
     6781 ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt
     6841 tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat
     6901 cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa
     6961 tctacagttg catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc
     7021 agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca
     7081 attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat
     7141 gatgccggaa gatatacgat attccagttt tttactgttt atggagattc aacgggaatt
     7201 gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac
     7261 ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa
     7321 gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact
     7381 tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt
     7441 caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc
     7501 gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta
     7561 caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga
     7621 gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata
     7681 aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt
     7741 tcatgacctc tcgataaacg g
//
LOCUS       DACCQI010000121         7524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCQI010000121 DACCQI010000000
VERSION     DACCQI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.125"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(542..1888)
                     /gene="gor"
                     /locus_tag="IVO76_002379"
     CDS             complement(542..1888)
                     /gene="gor"
                     /locus_tag="IVO76_002379"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7430316.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2022..3263)
                     /locus_tag="IVO76_002380"
     CDS             complement(2022..3263)
                     /locus_tag="IVO76_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7430317.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3281..4204)
                     /locus_tag="IVO76_002381"
     CDS             complement(3281..4204)
                     /locus_tag="IVO76_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7430318.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4425..6917)
                     /locus_tag="IVO76_002382"
     CDS             complement(4425..6917)
                     /locus_tag="IVO76_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7430319.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6924..7391)
                     /locus_tag="IVO76_002383"
     CDS             complement(6924..7391)
                     /locus_tag="IVO76_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7430320.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2071 a         1637 c         1206 g         2610 t
ORIGIN      
        1 tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt gccgtttttt
       61 gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt gactaactat
      121 gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca gcgaacttga
      181 cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg tttgcttctt
      241 ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata catttctatt
      301 tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat ggattcattt
      361 actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat ttcgttttga
      421 gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa aatcagtata
      481 ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg ttaatattaa
      541 attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact gtattgtcaa
      601 agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc atctcatcga
      661 caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca cagatcaatt
      721 tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta aagcgtgaac
      781 gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct gtcaaaccaa
      841 ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag tcaagataag
      901 aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg attgcaacag
      961 gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca tccgttgtat
     1021 tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt gtattttcca
     1081 gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa tcaccagtta
     1141 aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta atttctttag
     1201 caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc aaattgtccg
     1261 aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac atgacttcac
     1321 tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca cctaaaacga
     1381 ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt gcgtattctc
     1441 caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt ttaggagcac
     1501 gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg taacctttta
     1561 tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga aaatcaatat
     1621 atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct gcttggatac
     1681 cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc cacataactt
     1741 ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct tcaatcagca
     1801 aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca gagccgccac
     1861 caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag atcaatcaaa
     1921 acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc ttgaattttg
     1981 tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg ttttcggatc
     2041 atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat tggatttggt
     2101 gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg actgaaatga
     2161 ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt tctctgacgt
     2221 atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa tttatatact
     2281 gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt gtagtaaaaa
     2341 cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc ttgcttgcag
     2401 acatagtttt caggatcttc catataactg tctacagccg ctttatagac atttgacacc
     2461 gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc tacgccgttt
     2521 tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat cgagaattct
     2581 tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg catatcgaac
     2641 aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc ccgcatcgac
     2701 atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc atggataaat
     2761 gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat tgaaacttca
     2821 cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt ctcaaaatta
     2881 gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt cgcacaaatt
     2941 tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg caaactgcgg
     3001 aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc agcaacgtat
     3061 accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc ataagtaaaa
     3121 ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc atctgctcca
     3181 taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat ttctggtcgt
     3241 ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata gcagaaggat
     3301 caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta ctgatttcgc
     3361 tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt ttttttgctt
     3421 cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat ccgtccaatt
     3481 tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga ttcagatcat
     3541 tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt gtctcttctt
     3601 ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct tgtttcgtat
     3661 agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta gcaccataca
     3721 caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct tcaaaaggaa
     3781 cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg atttgacgtg
     3841 cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca ggattttttt
     3901 tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt atttctttta
     3961 aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc ccatttacag
     4021 caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc tgttcttctc
     4081 gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat agtgtatctg
     4141 cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt atgagttcga
     4201 tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa ctttccaata
     4261 cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa aataaagttc
     4321 gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac aacctcttaa
     4381 tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag ggaacctact
     4441 gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc acctactgtt
     4501 acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg atcttcaatt
     4561 tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa tccagctttc
     4621 ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc tttcaatcgt
     4681 ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt actcaatgaa
     4741 tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc tttctttaac
     4801 tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg gacattgaat
     4861 ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat aatcatgatt
     4921 gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc atccaatact
     4981 tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa gagaatcact
     5041 gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata ccctacgtat
     5101 cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc tgacatatca
     5161 acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt ggccaattct
     5221 gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg atcaggatct
     5281 ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc ctcatcttga
     5341 ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct ttcttttttc
     5401 tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac atcttcttct
     5461 gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac tttttgcagc
     5521 tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa gtcttgtttt
     5581 tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat cacagttgat
     5641 tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc gatcaaatca
     5701 attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt gactgctgct
     5761 cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt acgtaatcct
     5821 tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg agcaaaacgt
     5881 cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt cgttgctccg
     5941 atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc atcaattgct
     6001 ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa gattacttga
     6061 ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc accgcgatat
     6121 ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc ttgcatgtct
     6181 tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc tgttttgcct
     6241 acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag aatctggata
     6301 agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc ttttgctaat
     6361 ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt ttgccgttta
     6421 tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc accagctagc
     6481 cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa gttgatcatg
     6541 attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg ttctgttcca
     6601 actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac ttgtttcatt
     6661 cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc ataaccaatc
     6721 agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg taatactttt
     6781 ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga actaactgat
     6841 tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg ggctctttca
     6901 gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg ttccagaaat
     6961 gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa tgtgtttttt
     7021 gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc ttcataaagt
     7081 ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa ctgatttatc
     7141 tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat ataaccacca
     7201 ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt attaatcaca
     7261 tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct tcgaatctcg
     7321 atcatttcac tattttccag aattttcttt atatatgatt caattaaatc cgaggtattc
     7381 tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata acgaatttga
     7441 taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca acaaaaaaaa
     7501 atacggaata tactattccg tatc
//
LOCUS       DACCQI010000122         7448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCQI010000122 DACCQI010000000
VERSION     DACCQI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.126"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..604
                     /locus_tag="IVO76_002384"
     CDS             <1..604
                     /locus_tag="IVO76_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7430321.1"
                     /translation="LIVQNLNYGTNQYSVDNSKFELCDTSMKTRDKNNFADSSKNELE
                     LYKSYKDSKDIKESHNDDLLESLSTKNNKLDEELIETFIEENLLKQIWGESIVNCLKL
                     ASNSDYRQFDNWYKKFKYAIRSAEKEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDL
                     IRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGIDKQ"
     gene            680..898
                     /locus_tag="IVO76_002385"
     CDS             680..898
                     /locus_tag="IVO76_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430322.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            885..3563
                     /locus_tag="IVO76_002386"
     CDS             885..3563
                     /locus_tag="IVO76_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430323.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            3554..3751
                     /locus_tag="IVO76_002387"
     CDS             3554..3751
                     /locus_tag="IVO76_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430324.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            3755..4366
                     /gene="mobC"
                     /locus_tag="IVO76_002388"
     CDS             3755..4366
                     /gene="mobC"
                     /locus_tag="IVO76_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAP7430325.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4351..5742
                     /locus_tag="IVO76_002389"
     CDS             4351..5742
                     /locus_tag="IVO76_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7430326.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            5958..6272
                     /locus_tag="IVO76_002390"
     CDS             5958..6272
                     /locus_tag="IVO76_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430327.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6265..6759
                     /locus_tag="IVO76_002391"
     CDS             6265..6759
                     /locus_tag="IVO76_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430328.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            6827..7141
                     /locus_tag="IVO76_002392"
     CDS             6827..7141
                     /locus_tag="IVO76_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430329.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            7240..>7448
                     /locus_tag="IVO76_002393"
     CDS             7240..>7448
                     /locus_tag="IVO76_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7430330.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
BASE COUNT         2859 a         1007 c         1435 g         2147 t
ORIGIN      
        1 cttgatagtt caaaatttga actatggaac aaatcaatat tctgttgata attcaaaatt
       61 tgaactatgc gacacatcaa tgaaaacaag agacaaaaac aattttgctg atagttcaaa
      121 aaatgaacta gaattatata agtcttataa agatagtaaa gatattaaag aatcacataa
      181 tgatgatctt cttgaatcac tttcaactaa aaataataaa ttagatgaag aattgatcga
      241 aacatttatt gaagaaaatc ttcttaaaca aatatgggga gaaagtattg tgaattgctt
      301 aaaactggct agcaatagtg attatcgtca attcgacaac tggtacaaaa aattcaaata
      361 tgcgattaga tctgctgaaa aagaacaaaa aattcaactg aaaattatct atgaaatttg
      421 taataacaag tattttgtag atcatgttag agaacagcta tcaatgactt tacgtgactt
      481 gatacgaaga gcaaaaacag atcatcgtat caaacaaaaa gacgattata tctttgcttc
      541 tctaaaaaat aaagcgttag aactaattga attgcaaatc agcgaaggaa tagataagca
      601 ataagaatag aattgagtca aatgaaatgg aggggtatct ccacttcatt tgagaacttt
      661 gaatatagga ggggttattt tgattaaaaa tcgtttaata gatcaagttt ggtgtctaga
      721 tcaaaataca gatagtctcg aattaatcga gactatttgc ttcaacacta tagttctcga
      781 tttaagagct gaaaatgata attgtgtaac tcatgtcata attaatcaaa aaatggctca
      841 gaagctctct gagagcctca gaaaatgggc taagggaggt aacgatgaaa ataattgaaa
      901 aaaagattgc caataaaaaa ataaattggt tggttgttaa ttttcttgtt ctgatagcta
      961 tgattatttt tacaagtaat attgctcatg cgagtatttt caattggttt gatgacacaa
     1021 aaagtcaaac agagtttttg aataattctc tttatagacc ttacctaaaa aagtacaatg
     1081 gagatttcct tagtcagttc gggatttggc tcggatgggc tgtggtaaaa ggcatgttta
     1141 ctgtgacaga ttcgattcaa aatatgattc cagatgtgct ggatttgttc aatttcattg
     1201 aaagtacagg gctaaataat gtttatcaat cagccgtaaa tacaattgtc attggattaa
     1261 tgatcctttc attgatgttt gttggttaca aaatgattac tggaaaagga acagtggatt
     1321 taaaatcggt tggaatgaac gtagtgatgt ctatagcatt aatactttta atgccaacca
     1381 tgatttctag tggtattcaa tttgctaaaa cattttataa ggactctact acgattacaa
     1441 attcagatga cggagtcgct tggtcattga taaaacaagg agtaacagat ttagcatata
     1501 ttaataaaac agatcagtac tcaagcattg ataaaacgga ggatcgaaat aatttaacac
     1561 gaaaaaattt tcaacagacg gatctcacac aagtgttaac ggataaagtg attgataaat
     1621 tggaaaaaga aaatccaatt gcggacaatc ttcgttatga attagttgaa aattctaata
     1681 atgaatttgt tgctacaaag ttttctgaca acttcttatc aacattttct gatagcttga
     1741 aatcaggcta ttaccgatac caagcaaatc tatgggggat ttcaattggg ttgacagcgt
     1801 tagcaatcgc ctatgtgttc agcgcgtttg tgatcattac agcaatacta gaacttgcat
     1861 ttaaacgtgt gttaggtgtt cttgtttttg caacagatat tgagacagga cagcgttcaa
     1921 aagttgttct atcagatatt ctacaatgtt acttaactgt tgggtttcaa ggatttgggt
     1981 tatcaatgtt tgctatgttt atcaattttc tgaactcagg acaaggtatc tcaacaaata
     2041 tttttataaa aacaattgct tatatctgtg ccgtttttgt tttaatcaaa ggcagtggaa
     2101 cagtcatgag atactttggt gttgatattg gactcaaaga aggctatggg caacttgctt
     2161 ctgcttttgg aatgggcgca atgctgttta gaaaaggatc aaatggattt aatcgtgcta
     2221 aaggaagcgg agaaggagaa gatcgaaaac cagaaaagaa tttcggcgaa actctatcaa
     2281 aaaaagctgg caaaactgga agagctttgg gatatgccca tgaacgtgga ttatccggtt
     2341 tagcttcaga tggagctaca atggctagtg aaagagcaac aaaaccattt aagagtatgc
     2401 gtgatatagc taatgatacc aagaataagt ttaaagaagg tttagatgat ggtacagtgt
     2461 ccgctattaa taaaaatagc aaacctatgt tagctaaaaa taaagaagat gaaactggaa
     2521 aatatgctga ttccgtgcca acacgtcttt cagatagaaa agatggtgct aaagttgaaa
     2581 atgccgatag aattatgtct agttctgaac gtatgcgtga agcaatgaaa aataatgctg
     2641 aatcgaataa taatcctgca agcgcaattc agcaaaaagt ccaacaagat attgaagaac
     2701 gtaaaaatgc tatgcacggt caagctaagt ctgctgaaga attaattaat cagaagcgtc
     2761 atgaagcgaa atacacgcct gaagcaatga atcgcgagga aataatgtta aggaaacgtg
     2821 tagaagggcg gacaggcgct aatattgacg gaaaagaagc gttgaccaaa gaaagaattc
     2881 aagaagctaa aaattcaaat acaggattag aaaaattagt caaagaaaat ccacaaaatt
     2941 ctacaagtag tcatggtggc cgatcagtag atgtccgaga aaatattcaa ggttcactga
     3001 atggtcatgt tggacggaat gttgatgtaa gagagaattt acaaagttct tcaaatgctc
     3061 aaggtactaa gacaattgat gttcgagaaa atctgcaaag tagcagtaca agtcaaccaa
     3121 aaacagtaga tgttcgagaa aatgtacaaa attctacaag tagtcaaggg tttaaatcag
     3181 tggatgtccg agagaatgtg caaggtagca gtgcaagtca accaaaaaca gtagatgtcc
     3241 gagaaaatgt acaaagttct acaagtagtc aaggattcaa aacagtagat gttcgggaga
     3301 acattcaaaa agatagtgga gaaatgaaag aaaaaacaca aaaaatcaat atcgttgaag
     3361 acagaaagtc agcgaaaaaa tttgattcaa atcatgaaac agtgattatt gatagtgaaa
     3421 tccgtgaaaa tgataaggga tcaaaatcaa gacgaagatt cacttatgaa gacaatgagt
     3481 tattcagaga tacacttaat gatcctaatc cacttttcga taatttatta agaaataaaa
     3541 gggggggatt gtcatgcaat taattctaaa gattagtacg catgatttct tagaacttga
     3601 cgaattatgt gaatcagtaa agaacgcaac aaatgaaaaa atcacggtca ctaattatat
     3661 tgaactgatg attcaagaag aattaaagat gaaaaatgaa atagagagca atttcaaatg
     3721 ttgctctcta gaaaaaaatg tgaggggata atcaatggat gataaacaaa aaatgtacga
     3781 aaaaaatcgc aaacatagtg aggccttaaa ttttcgtgtc gataaaaaga cttatcttga
     3841 actgacattg attgcaaaac aaaggggaat ttctagagca gacgtttgcc gtgagcggtt
     3901 aaagggactc aaatatgaac cgccgttgtt agctgaagat gatagggtag ttttaaccaa
     3961 acagttaaat caaattggta acaatttgaa tcaagcggta aaggcattaa atatcattgc
     4021 taaatatttt agagaagaat ctgaagataa aaaagaaaag agcctacatt ttagttttga
     4081 tgctagccgt tacaacattt ttaaagatga acttgcgaat agcatagcaa aaaagttgat
     4141 gattagttat ccagatgaca tttttttgga gtccaatcgg agtgccccct ctttttttaa
     4201 attatcagaa gtagaaaaag aatatttcga atattggata gaaaagcaaa ctagaccggg
     4261 tacgaaagaa gagtattccc aaaaagttga tcggaattta attcactcaa ttcagattat
     4321 tgcaagttta aaagatgagg tgaaacaact ttgggagcta ctataacatc gaaaggacgc
     4381 aagaaaaatt tatcctcgct attacaatat gcagctaatc cttcaaaaaa agaaaaatgt
     4441 gttgcagtct caggaataaa ttgtagtgat gatagtgagg aagcactgca agaaatgaat
     4501 ttagtacgta ccaagatgaa taaatctaaa gatatagcac aagcgtttca tgttattcat
     4561 tcgtttgata agagtacttc taaagagtta agtattgata aaatgcacga aatagctgtc
     4621 gaatttgcag agaaagcttt tccaaattcg caaatcattg ttgcaagtca caatgataaa
     4681 gatcattttc atagtcatct tgtcattaat aatattaaca tggaaacatg taaaaggttg
     4741 cgtattgacc ctaaagattt agaactaatg cgaaaaatta acgatcaagt cctcaatgaa
     4801 catggtcttg aatcagtgaa taatgagtac tatgaaaact tagaaaagag aaccaatctt
     4861 tattcaaacg atccttctaa gatgaaatat ggaaaaatta attatcaaga gtttgtgcaa
     4921 aatgcgatcg aagcaacgtt ggaaaaatgc gaagtgaatt cttttgaaga tttttcggat
     4981 catttggcaa aagaatataa cattgaagtt tatcaatttt caaaaacaaa taacaaactt
     5041 ggctatgttc tttacaaaca aggtgtaaat ttctatgagc gtagaggtga gttaattact
     5101 ttaggaagag ataaacaatc tagaaaagaa aaaagtcagt atgtcgaacg tacttttagt
     5161 gcaagaaagt tagggaaaag atattcattt gaatctgttt ctgaacaact tgaaaagaat
     5221 ttagaagaat cacatataaa aaattttttt aatatggaaa gagagcaagt tatgtcgcta
     5281 attagtggag taaattacaa aactggtgag ttaaatgtac cgtttgtgga attagattcc
     5341 atcgcacaaa agcaatttag cgaccgtgaa ggcgatgaaa aattcattaa atatgatgac
     5401 ttcttgcgag aggtagacaa gcattttgta aatacaatgt ctttagaaga tgatcaagaa
     5461 tacacgatta aacttcatac ggatgtgaat cctcaaactt tacgtagtgc gaaaagtaaa
     5521 acaatttcta ttaaaaagac tgatcagaaa gagttatttc taccggcaaa gggttttgaa
     5581 aattgttctc atacaccttc tgctaaacag attcactatc aaatcagtcg cataatgaat
     5641 gacgatgtaa aaatgtatcg tgctaaaaag gattatgaat tcacacaaca aatgattgaa
     5701 aatcagcaaa aaaggcaaca aaattttgaa atggaattct gaatgggctt gttagtggac
     5761 tttccagttt tttgcctatg aagagttgct tggaaagaag cagtttttag gacatacgcc
     5821 agctacccat ctacttgcca gtcaatccgc cccgctcacg ctaggctact ttctggcaag
     5881 tcagatggcg ggtaaatctc tgagggagca agctccctac agactccctc ccaaaaaaag
     5941 aaaggtggaa gaaaaaaatg aactatccat attttaaagt ctcggcttca gaagaaacta
     6001 aagaaatatt caacaatttt tacaaccaaa acaaaggaat ttttggttca aaggcaaata
     6061 tgtttcgtgt tatggtttca aatttaccag tgttagctag tccatcaaat aataaattta
     6121 atgatcctga aagcattaaa tttgaacaaa aaatttcaga actagaatca atgataagta
     6181 atgaagtaat tgaaaagctg gatgacattg atcaaaagtt atcgtattct ctaaaaaaca
     6241 aatacaaaac ggaggagaaa aaagatgttt aaagtttact tcccgtttca gtggctcgtt
     6301 aaaggaattc ttttagtgct actaggattg attctttttg gaagttatct aacaattaaa
     6361 gccagacatt ttccagaaga aaagccaata aaaattgtaa caaaagattt aaaaataggt
     6421 gaaacttatc aggcagatgg ggttgaacta acatttaaaa atattgaagt gaccgaaaaa
     6481 agaaatgaat ttgcacaaca tgatcgtgta ttacaagtga cctatacact tataaatgat
     6541 acagaaaatc cgatacgtcc acaacaatgg tcgattgaaa ataaggatgg tgaagaactt
     6601 tatgagtatg atatgatatt ggataatcca gagtatattg aaagtggcaa agaagaaaca
     6661 gtaaattact attatgaatt accgattgaa aggcaggact accaaatcgt agtacgtaga
     6721 gagaatggtt acatagttgg gttgccattt actttataag ttttattttg ttagtagttt
     6781 ttaaggtaat atcagttttg tgatttttga aaatggaact gggagaatgg ctgaggaaaa
     6841 taaagttgtt ccgtttgaaa cattagtgat aaataaggaa gagtttgagc aactttttct
     6901 tgattttttt attcaatatg acatgattta tgattttagt tcgtcactag atttatttga
     6961 attagaagca gaaataatag aaaacagtcg agagaagaat ggtaaaatca ttatttcagt
     7021 agaaaaattc aatgaattat tctcacaaac atttgaaata gatccagact attatcaacc
     7081 acaagaagtt tttcgagagt ttagaaataa gattattggt tttagtcaaa ctacaaaatg
     7141 aaagcttaat aaagcaagtg ttgatagaat tattcctcaa cgcttgcttt attttatggg
     7201 cacttcataa tagaggtata gagaagagag gtattcattt tggatactat tactccaaca
     7261 aacttcaaag aaaatatagg taaagtgttg caagaagtag ttcattcaaa ttctgaaata
     7321 gaaattacta gggattctaa aaatggattg aacgatggcg tcattatgat ttctaagaga
     7381 aaatgggagt tgttacaaga agaactttat ttacaacgaa cgggtacatg ggcttatgta
     7441 tctgattt
//
LOCUS       DACCQI010000123         7417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCQI010000123 DACCQI010000000
VERSION     DACCQI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.128"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(90..1430)
                     /locus_tag="IVO76_002394"
     CDS             complement(90..1430)
                     /locus_tag="IVO76_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7430331.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1699..2595
                     /locus_tag="IVO76_002395"
     CDS             1699..2595
                     /locus_tag="IVO76_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7430332.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2745..3686)
                     /locus_tag="IVO76_002396"
     CDS             complement(2745..3686)
                     /locus_tag="IVO76_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7430333.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3812..4933
                     /locus_tag="IVO76_002397"
     CDS             3812..4933
                     /locus_tag="IVO76_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7430334.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5191..6693
                     /locus_tag="IVO76_002398"
     CDS             5191..6693
                     /locus_tag="IVO76_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7430335.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6760..7287
                     /locus_tag="IVO76_002399"
     CDS             6760..7287
                     /locus_tag="IVO76_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7430336.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2498 a         1358 c         1487 g         2074 t
ORIGIN      
        1 aaaggagttg caacaagact tttgtcacaa ctcctttctt caatagctcc atctattcca
       61 cataaataaa ataaaatttt ctatcgattt cattcattcg tcaatacgaa cgcatcattc
      121 tttttttgat aactgaaata aatgaacagg taaagcaggc cagtcaatac agttacccca
      181 atggagttcg tgaacaggat accaaggaaa atcaatccta agacagctaa tgcaaaaaag
      241 ctgattcctt tatcccataa tgttcctttt cttagtgcca gtacaaagga tgccgcactg
      301 ataatgatcc agatcaataa tactgtatac gaaagggaac ctgccagata gccaaataat
      361 ttgtcaccga caaaataaga aagagctaca ccaagataaa gaacactggt acaaaataaa
      421 acagcacgtt gcggtacttg atgcttgttt aaaacagcta gttttctaga ggaacctttt
      481 cgatccttca aacggaagaa caaaattctt gaagatgcat acacacccga attgatagat
      541 gagaacaaag caaggacaat cacaaaattg acgatatccg ctgcaaatgg gatattcatt
      601 ttttcaaata ccatcacaaa aggactgaca ttcgtgcctg ctaaagtatt ccaaggaaaa
      661 agaatcaaaa gcaaaaacaa aggaatgatg taaaaggaaa tgattcttcc catgacacct
      721 ttgattgctt taggaatcgc acgtttagga tcatctgctt cgcttaccgt gatagcaatc
      781 agttcagaac cgccgtaaga atagataact acaagcaaag aactgatgat tcccttcata
      841 ccatgaggag taaacccacc atggtcagtc aattgctgaa gggtagggaa aacgcctgtg
      901 cccaacattt gctgaccgac aagataagta gcaaaaatga tcagtaagat aatgacactg
      961 attttagcaa aagccagcca atattctgtt tctgcgaata accgtaccga ataaagattg
     1021 atgatcgtag ttaaaacagc caacataaaa acaaataccc aggctggaat gtgcgggaac
     1081 cacaactgca aaaaggaagc agctgcaaca gcttcagcga tgatattgat aatccacata
     1141 gaccagtaga cccaatcagt aaaatcagca gaatgagcac ctagataagg gcgtatcaaa
     1201 ccagataatc cgtgatgcgt gtcgccaccg cttaaaacga tttttcccag acctttcatc
     1261 acgatatata aaatcagccc accaatcaaa aaagcaatca atacagaagg gccggcgatc
     1321 ccaatcgctt cgccacttcc tttaaataga cctgcaccga ttgctccacc taaagctaac
     1381 atggtgatat gtctggagct aagttgtttt ttcaattttt tatcttccat aaatgacctc
     1441 tttcttttaa attcacaatc aaaatttata ttaagaataa atagcaatat ttaacaatga
     1501 ctaatgataa caaattttca gacaattaca actattcaga aaacttttag gcgaatttta
     1561 atagaagaat gatttatttc atcatatttt catacaaatg ttaaattttc atacaaatat
     1621 aggaaacgtt ttactggctt tttactattt aaacaggcta tcataggaaa aagtgacaga
     1681 gaaagtgagg cggaaaagat gaacaaaaat gacgtactgc tagttgcaaa aaataaatca
     1741 ctttattttt tgccttccat ccgctcttta gatgtttggt ccggaaaaga tttactacag
     1801 tcggaattca ttaaatcagc tagacaacag ttggcaaatt atcagctgat cgttttttta
     1861 gattatggat ttgatatgga gatggctcga cgcgtgcgcc catttaccaa tgcaaaaatt
     1921 gtgttatttt tttggaatca cttcaaagca gaacacgagg cgatgctgaa gcaggcacaa
     1981 gatgaaccgg caatcgatga gatttatcat tttgattttt tggaagcaaa agaacttggg
     2041 ctgaaacata atagttcatt ttattcgaaa catatgaatt tttcttctag cgaaccaaag
     2101 acggatctat tttttggtgc aacgaataat gggagaaaag aacgagcaga aagctataag
     2161 caggaattta taaaaagaga tgttacgacc aactatttca ttcttccaca tcgagggaat
     2221 gaacaacctg gatatcttac ttatgaagag tacctgaaaa aaacagggga atcgcgcggg
     2281 attctggaac tgttgcggga gggtcagcaa ggagtgactt tgcggacatt tgaatccatg
     2341 tattttcaaa agaaactagt gacggataat caagcgattg ctcattatca tttttatcgt
     2401 cctgaaaata tttttttgct tcgagaacgt gatttagatg aattaccgga atttctgcat
     2461 acaccattta agccaatcct atcagagcta ttggaatttt atgatgcaga aaattgggca
     2521 aaacgattta tcactaacga ttccactatt tatgagcaat acgaatatga cctctcctct
     2581 gttgaaaaca aataaaaaac ctacattttg aagagattat gtactgaaac aaacaagcac
     2641 aattcttatg gcatcatgag agttgtgttt tctttttgat cttttattgg gcttaaaaaa
     2701 agagaaccag ctgctgattc agctgattct catcagagag ccttttattc tgcgccaagg
     2761 attccttctt ttgcatggac taattgggtc aaaaaggcac aatatggtgt agcaatatca
     2821 tatttttgtc cttttcgcca aatggcacca ttgatgtagt cgatttcagt caaacgatgg
     2881 tttttgatca ggtcttggta catagatggg taatgcaacc cgatgttgtc aggatcataa
     2941 gtagaagcaa tatgttggta cacttcttct tgatctagta tgattccttc ttttgctgcg
     3001 atagaagcaa attcagatac gattgttcga accatgctct ctgccgcttt ttgcgcgcct
     3061 agttcagcaa tattacaatc caaaatcgtg cataaaccat tcaatgttcc atttacacaa
     3121 gcttttcgcc aaatggaata acgaacgtta tggctataga tcggatttaa atttgcttct
     3181 tgaaagactt ctacaacttt ttttgtaaat gcttcgcctt ctggttccaa gttctctagt
     3241 tcgatttctc catcacctaa caatgtgact tttccgggac cagctagacc agctgtccac
     3301 atcgtgatcc caaataagat atttttcttt ggcacatatt tttccagtac atcttcatga
     3361 cctaatccat ttagtaaaca caaaacataa gtattgtcag taataatgga ttggatcgaa
     3421 caaaacatat catctaattg attggctttt gttaatgcta cgatcaagtc cacgtgttcg
     3481 ttgctttcga tgatttcttc tggtgaatag attggcagtt ttgcgaccac ttctttaccg
     3541 ttaaagtcag caatcaaccc atttgtacga atggcttcaa tgtgagcagg ccaacgatcg
     3601 atcaagagga cctcatttcc gctttgatgt agcatcaatc cgattcggct gcccattgct
     3661 ccggccccag ctattgcgat tttcatgttt tttctctcct ttattcaatg agttcccttt
     3721 tagtatatag aaaaatggaa gaaaatagca ttgttttgtt tgaaaaaaat gaaaaaaagt
     3781 gtcttactta agaagaaagt gagggataca gatggatatt ttggcggcaa ttgattcatt
     3841 taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct gggaagatcg
     3901 taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg cagaagcaat
     3961 ctattacgcg ctcaggggaa catggcgtac cattgatggg atggatctct tattccgtca
     4021 gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa aacaccgcag tcatcgaatc
     4081 tgcacaagca ttggggttag atctacttga taaaccaacg gatcaaacta tccgacttgc
     4141 ttccagttat gccttaggag aacttattaa agatgcactg cttcaagaag tagaacaaat
     4201 tattgtcact ttaggaggaa gcgggtgtag cgatggtggt ctaggattac tccaatcctt
     4261 gggtgcttct ttagaagggt acacagaagg aaatccttta ttatctacta aaaaacttca
     4321 ttttgatcga agaaacgttc ttgtttctaa agtaaaattg attatcgctg cagatgttac
     4381 caatccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa aaggaggaat
     4441 agatacgacg ctagctttct tagataaaca agcaggaaag attgctcagc aaataaaaga
     4501 tttctgggag gtggatttag atgatattcc aggaagtgga gcagcaggcg gtattggtgg
     4561 agcccttttt ttgttgggag cgaagatggt ttctggtttc gatttagtca gccaaatcac
     4621 tggattggaa aaaaagatcg aacaagcaga tgtcatcatt acaggagaag ggcggatcga
     4681 tcagcaaacg atccacggaa aagttccata cggtgtagca gtgcttgcaa aaaaatatcg
     4741 aaaaccaata atcgctattt gcggaagtag agaccaagag ctagaaggac tttctgattt
     4801 tctcccaatt gtcttaagta ttcatttggg tccaataggt ttagaagaag caatggaaca
     4861 caggcaaaca ttagaaaaaa caaaaatctt aggacagaca cttagcagat ttttcaccat
     4921 cctcccgaag tagttgacaa aatcattttc tttatgctat cgtaatttca aatataggac
     4981 aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc attcagactg
     5041 taccttgtgg agacaaggaa gcagtgtgca gattttcggg attggcttgg ttttttcgat
     5101 gtatgggtct ttttgtgtgt atccaaccct tgcatcctct ttagccagtt ctgaaaatag
     5161 gactggtctt tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca
     5221 ttcggctacg acagccaagg cacattatta tttgaacaag caaatctaaa ttttgacaca
     5281 caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt actgaatatt
     5341 ctacaaaaca aactacctta tcaagggcaa gtaatccatc agcaagaatt tgcctatttc
     5401 ccgcaacaga caaaagataa agaacgttta acctattacg tgttaaatga tattacggat
     5461 tttgagatat gggaaatcga aagagagctc caattgatgc aaacagatcc tgaaatctta
     5521 tggagagaat tcagcacact atcgggggga gagaagacaa aagtcctact ggcactttta
     5581 tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt ggatatctct
     5641 ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc
     5701 catgaccggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa
     5761 ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact tcgtgatgaa
     5821 tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca
     5881 gctgaaaaag ccgaatggtc tcgttcccga gaaggagata aaacaaagaa acaagtcgga
     5941 ttcatcgata ctgaatctag acgagtgaat aaaggagcag tgggtgctga tgctgcacgg
     6001 acgatgaaac gatccaaagc aatcgtgaat cggatggaga cccagatcag cgagaaagaa
     6061 aaactattaa aagatatcga atatatcgat tctttgacga tgaatagcca agcgtctcac
     6121 cataagcgac ttttaagcgt agaagatctt caattagggt atgaaaatct gttattcgag
     6181 ccaattcatt ttacaatcga gcctcatcag cgggtggcga tttcaggtcc taacggtgca
     6241 ggaaagtcat ccattatcca ttatcttctg ggggcattca acggcaaggt tataggagaa
     6301 aaataccagc caaaacatct gagcattagt tatgcaagcc aaaattatga agacaatcga
     6361 ggaacgttgg cggaatttgc agagaaaaac caagtagact accaagcatt tttgaacaac
     6421 ctccgaaagc ttgggatgga aagagatgtt tttcataaca agatcgagca gatgagtatg
     6481 ggccaacgga aaaaagtgga attggctaaa tctttatcac agccagctga actatataca
     6541 tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga acaactgatc
     6601 ttgaacgtga aacctgccat gttactagtg gaacatgatc aaaccttcct ggataaagta
     6661 tctactgaga ttatttctct tgagagaatc taacccttgt aatctcaaga acaacaaggt
     6721 atggttaagg ggaactttgc caacgaagga gagaatcaca tgctttatgt aacgagacac
     6781 ggagaaacaa cttggaacgc acaaggatta gtttgcggac gggcagatgt gccgctgact
     6841 gaaaaaggac agatgcaggc acagaaacta gcagaaaagg tagtagattt gcctgttcca
     6901 atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca agcagttgct
     6961 gatcgtctca gcttgccgct gaccgttgat gaacgattgg tagaaatgga ttttggggat
     7021 tatgatggac tgccaagcaa agatgagaac tttcaaaaag cacgtttagc gttcgctgta
     7081 cgattcccaa atggggagtc agtcttagat gtgtatgcac ggatcgttcc tttattgaaa
     7141 gaatgcatcg aagacgaaga aaatgtctat ctgttagttt gccataatgc cttgataaga
     7201 gtgatcaatg cttattttca tccgatgcct aatgaggggt tctttacatt catggtagat
     7261 aatacagaat tggtttctta tgagtaatta tgtttttgtg ttatttaaat aaaatatttc
     7321 aaggcactgc ttttgcttag cagtgccttt tttaggttat gacaattctt ttgataatat
     7381 aaactcaacc tttagaaatg aaatttaatt attttaa
//
LOCUS       DACCQI010000124         7396 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCQI010000124 DACCQI010000000
VERSION     DACCQI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.129"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            245..892
                     /locus_tag="IVO76_002400"
     CDS             245..892
                     /locus_tag="IVO76_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7430337.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1285..3930
                     /locus_tag="IVO76_002401"
     CDS             1285..3930
                     /locus_tag="IVO76_002401"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7430338.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4240..5553
                     /locus_tag="IVO76_002402"
     CDS             4240..5553
                     /locus_tag="IVO76_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7430339.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5543..6196
                     /locus_tag="IVO76_002403"
     CDS             5543..6196
                     /locus_tag="IVO76_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7430340.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6251..6943
                     /locus_tag="IVO76_002404"
     CDS             6251..6943
                     /locus_tag="IVO76_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7430341.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            7093..7293
                     /locus_tag="IVO76_002405"
     CDS             7093..7293
                     /locus_tag="IVO76_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7430342.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2441 a         1259 c         1621 g         2075 t
ORIGIN      
        1 aagttacttc tggaacaccg tgtatcagag gttgcgaaaa agctgccctg catcaagtaa
       61 taagaacagc caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc
      121 agatgcaagc ttttgaacac cgttttattt gtctcacacc cttttccttc ctacttgtaa
      181 ccttgctccc gtgttagaaa catgttacac taaatatgtg aaattattaa caaggagcgt
      241 cgttatgcga gaaaaaacag atacactgcc aaaagcaact gcgaaaagat tacctttata
      301 tttacgctac ttgaagatgc tggatgactc aggtataagt agaatcaaat caaatgagtt
      361 cagcgaaatc acccaaattc cttctgcaac gatccgcaga gacttctcgc aattaggcga
      421 gctaggcaga agcggatatg gctatgatgt cccttttctt atcgatgtat tcaataatat
      481 ccttaatact aaagaagaaa aacgaatcgc attagtagga tatggaaatc ttggcaaagc
      541 attgaaacac aacaatttta gaagaaatga aaatctaaat atcgtgtgcg tctttgataa
      601 tgatccagca ctgatcaatc gagtaattga tggagaaatg atctatccga ttgatcgttt
      661 cgcggagata gccaaagcaa aaaatgtgac agtagcaatc tctacggtac caagtaaata
      721 ttcacaatct gctattgatg aaattgtcaa aggaaatgta accgctatac ttaattttgc
      781 gcctgacaga gtaactgtcc cagcttatgt caatgttcaa tacattgatt tgacaaccga
      841 gttgcagacg cttatttact ttgatgagaa ttactcagaa gttttttctt aggaaaattt
      901 gagtaggctt attgatcttg cgagaactcg tcgtaattct ttcaaaaatt ttttagtact
      961 aaatgaagtg gttttttcac gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga
     1021 agcataacgc tgaacaagag gagtaactgg cacattcatt cttagagaga gaactatctg
     1081 ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg agtttctgtc ttgaatggag
     1141 tagagataga cgagtcgtga tcgttaactc acttgagagg caataatgat tgattattgc
     1201 aatttaggtg gtaccgcgta aatcatacgt cctagaatac gaaagtattt taggacgttt
     1261 tttagtttaa aggaggaaaa cagcatgacg gaagaaaata acctatcaac aaaatataat
     1321 ccgcaagaag ttgaagctgg acgttatcag cattggttag aacaagattt gtttaaaccg
     1381 agcggagata aaaaagcaaa accttattca atcgtaatcc cgcccccaaa tgttactgga
     1441 aaacttcatt tagggcatgc ttgggatacg accttgcaag atatgctgat tcgtcaaaag
     1501 agaatgcaag gatatgatac tttatggctg cctggtatgg accatgcagg aatcgcgaca
     1561 caagcaaaag tagaagcgaa gcttagagag caagggattt ctcgttatga tctaggtcgt
     1621 gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt atgcgagcca catccgcgaa
     1681 cagtgggcta aaatgggctt atctttagat tacagtaggg aacgttttac attagatgat
     1741 ggattatctg aagcagtacg taaagttttt gttaccttat acgaaaaagg tttgatttat
     1801 cgtggtgaat atatcatcaa ctgggatcct caagcacgaa cagctctatc tgatatcgaa
     1861 gttattcata aagatatcga aggcgccttt tatcatatga gctatgaact agcagatgga
     1921 tcaggcgtag tagaaattgc gacaacccgt ccagaaacga tgttgggaga tacggcgatt
     1981 gctgttcatc cagaagacga gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct
     2041 ttagtcaata aacaaatccc aatcattgca gatgaatatg tagatatgga attcgggaca
     2101 ggtgtagtaa aaatcactcc agcacatgat ccaaacgact ttgaggtagg taatcgtcat
     2161 gatcttccac gcgtaaatgt tatggatgat gatggtacga tgaatgaact agcaggaaaa
     2221 tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc
     2281 ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg gtcattccga gcggacaggt
     2341 gtcgttgttg aacctcggct gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa
     2401 aaagccattg ataaccaaga aacagatcaa gcagtggaat tcttccctcc aagattcaat
     2461 caaacgtttt tacgttggat ggaaaatgtc catgattggg taatctcacg acaattatgg
     2521 tggggacatc aaattcctgc atggtatcac aaagaaacag gtgaaatgta tgtagggatg
     2581 gaagcaccag cggatagtga aaactgggta caagatcctg atgttttaga cacttggttt
     2641 agttcagctt tgtggccttt ctcaacaatg ggctggccag atacggaaag cgaagattat
     2701 aagcgatact tccctactag cactttagta accggttatg acatcatttt cttctgggta
     2761 agcagaatga ttttccaaag cttggaattt actggcgaac gtccatttga gaatgtcttg
     2821 attcatggat tgattcgtga cgaacaaggg cggaagatga gtaaatcatt aggtaatgga
     2881 atcgatccga tggaagtaat cgaaaagtat ggagcagatg ctctccgctg gttcttatcg
     2941 aatggttctg caccaggaca agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg
     3001 aactttatca acaaaatctg gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg
     3061 accgtagaag agatcgattt atctggagaa aaatcagtgg ctgaccggtg gatccttact
     3121 cgtttgaatg aaacgatcga acgagtaact gagctatttg atcgttttga atttggtgaa
     3181 gcaggtcgac aactgtataa ctttatctgg gatgattttt gtgactggta catcgaaatg
     3241 agtaaagaag tactttatgg agatgatgct gtttcaaaac aaaccacccg tagcatctta
     3301 gtccatacac ttgatcagat cctacgttta ttgcatccaa tcatgccatt cgttacagaa
     3361 gaaatatggg aacatattcc tcatcaggga aattctctag tagtggcaga atatcctgtt
     3421 gtacgtccag agtttgatga tgaaacagct tctaaaggga tggaagtatt gaaagaattg
     3481 attcgagccg ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa accaattacg
     3541 ttgctaatta aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa tacaagctat
     3601 atcgaacgct tctgtaatcc cgaagagttg acgatatcaa gtgagatcac tgcgccagat
     3661 ctagcgatgt cagctgttct gacaggtgca gaaatcttct tgccgttagc aggattgatc
     3721 aatattgaag aagaaatcaa acgattagaa aaagaactgg aaaagtggac aagcgaagta
     3781 aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta
     3841 gtagaggcag agcgtgcaaa agaaaaagat tatttagaga agcaaacggc tgtcaaagaa
     3901 cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa agagagtgtg aaatcagcta
     3961 gctattacta aaagctagct gattgaacat cgttacagga aagagtttga gcctcactct
     4021 tagcttcgat tcataaggag atgtgacata aattttgtcg catctctttt ttagtctact
     4081 cttattatgt aggagaagga gggaggtgga tcatgtatca agagtcaata aagtagttga
     4141 taaaatcgtt cctctctctg tttcttttct ctttgtcata cttttatttt tcttatgtgt
     4201 tctatttgtt ataataaaag agaaatgggg gaattgttga tgacgattga agaagcaata
     4261 gaatggatcc atagtcgatt accatttggt tcacgtcctg gccttgaccg agtcgaagct
     4321 ttattagaag tggtagggca tccagagaga aaagtaaaaa cgatccatat cgctggaaca
     4381 aacggaaaag gttctactgt agcttatttg agaagcctat tagaagaggc tgggctgaca
     4441 gtaggtacat ttacttcacc ttatatcgaa acgttcaatg aacggatcgc aatcaatggc
     4501 aaaagcattc cagacaatga tttgatccag cttgtaaaaa aatatcagcc actgatcaaa
     4561 caacttgatc aatttgaagc agtagctggt attactgaat tcgagacatt aacagcaatg
     4621 gcatttgatt attttttgat gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc
     4681 ggcttattgg attctaccaa tgtagctgta ccaatgctga cgggaataac aacgattgga
     4741 cttgatcata ccgaaatact tggagataca attgaagaaa tcgctgcaca aaaagctgga
     4801 atcatcaaac aaggcgttcc agtagtaaca ggaaatattg ccccagaagc ttttactgtc
     4861 atccaacaaa cagctaaagc aaaaaatgca ccgctttatg tatacgggga aaattataaa
     4921 ataaactatc tgcatcctga tgagatgtgg ggagaagtat ttgaattttt taatgaaata
     4981 ggaaagatcc ctcaattaac cactccgctt ttagggcaac atcaagctga aaatgcagga
     5041 gtagccatcg aattgtattt tgtttactgt caattggaaa agctgccttt ccgagaaaaa
     5101 gatgttttga agggattgaa gaaatctcga tggccaggaa gaatggagcg tttgagtaac
     5161 gagccattag ttgttttaga tggtgcccat aatacgcatg caatgaaccg gcttgttgag
     5221 aatttgaaaa aagaattcaa aggatatcaa atcaacattt tgttttccgc cttacgaact
     5281 aaaaatgtta gagaaatgct agaacgattg cagcaagtac caaatgtcca tatttatttg
     5341 acgacctttg aacatccaaa agccatcgaa ttaagtgaag tatcagattt agcaagtgag
     5401 agagtatcaa tcgtttcatt gtggcaattc ggattagcag atatattgga aaagatgaca
     5461 aatgaagacg tattagttgt gaccggttca ctttatttca tttcagaagt tcgccattta
     5521 ttaatacaat taggaggaga tcatgaagta taatgcagtg atttttgaca tggatggttt
     5581 gctgttcgac acagagttag tgtattacga agccacacaa gtagtagcag acaagatggg
     5641 ttttccttat gataaagacc tgtatttaaa attcttaggt gtttcagatg aagaagtatg
     5701 ggcgaattat catatgattt tctcggaatt tggcaaaaca agtgtacaaa gattcataga
     5761 tgattcatac caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg
     5821 tgtgttggaa ctcctctctt ttttagaaga acatcagatt cccaaagttg tcgcttccag
     5881 taaccaacga gcagtcattg aacttttatt gaagaagaac aatctgtttg aacgatttga
     5941 gacgattgtt tctgcagaaa atgttcaacg agcaaaacca gacccagaaa ttttcttgtt
     6001 agctaatcag aagctaggta cagcgaagaa agaaacattg attttagaag attcacaaaa
     6061 tggtatttta gcagcttctg cggctgagat cccggtagtc atggtaccag atctgcttcc
     6121 accatcccaa gaattgatca aaaaaacagc tggggttgtt tcttctttac atgaaatccc
     6181 tgcattgtta aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg
     6241 agatgttttt ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga
     6301 acgtatggaa atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact
     6361 gcgtaccggt caacaccctt atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt
     6421 tggaggactg gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat
     6481 aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac
     6541 ggatcatgaa agagcggctc caccggttcg taccagctac gaattggccc agcaattgat
     6601 tctggaaatg aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa
     6661 tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc
     6721 tagagaaatt ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa
     6781 tcatccttct ggtaatgtta ttccatcgca gcaggatatg aattttacca aaaggataca
     6841 aaagtgtgga gaaatgatgg ggatcactgt actggaccac ttgatcatcg gacgcaaacg
     6901 atattttagt ttgagagaag aaggaatgat ggaagagaag taagttgctt tcttgcaaaa
     6961 aaaatgaagc aagtgtacaa ttatattgta attttgtttg ggaatagaag ttgaaacatc
     7021 tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa attagtgctt ttggcacgag
     7081 gaggatatta ttatggaaaa cggaacagta aaatggttca acgctgaaaa aggatttgga
     7141 tttatctcac gtgaagatgg aagcgacgta ttcgttcact tctcagctat ccaaggtgac
     7201 ggattcaaaa cattagaaga aggtcaagca gtgactttcg acgttgaaga atcagaccgc
     7261 ggcccacaag ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa attgatggta
     7321 gacatcaatt tttacgagag aacaaagcga gtaacaaaaa tacctgccac aagagacaat
     7381 ttctcttgtg gcaggt
//
LOCUS       DACCQI010000125         7352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCQI010000125 DACCQI010000000
VERSION     DACCQI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.130"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(58..354)
                     /locus_tag="IVO76_002406"
     CDS             complement(58..354)
                     /locus_tag="IVO76_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7430343.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            709..1065
                     /locus_tag="IVO76_002407"
     CDS             709..1065
                     /locus_tag="IVO76_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7430344.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1075..1974
                     /locus_tag="IVO76_002408"
     CDS             1075..1974
                     /locus_tag="IVO76_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP7430345.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2491..3222
                     /locus_tag="IVO76_002409"
     CDS             2491..3222
                     /locus_tag="IVO76_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7430346.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3367..5394
                     /locus_tag="IVO76_002410"
     CDS             3367..5394
                     /locus_tag="IVO76_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP7430347.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5502..6599
                     /locus_tag="IVO76_002411"
     CDS             5502..6599
                     /locus_tag="IVO76_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7430348.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6906..>7352
                     /locus_tag="IVO76_002412"
     CDS             6906..>7352
                     /locus_tag="IVO76_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7430349.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT         2556 a         1181 c         1463 g         2152 t
ORIGIN      
        1 atgttcaatc agctgacctt cggtattagc tcaaagcact gatttcacaa cctctattta
       61 ggcatgtgtc tcagcaagcg gttgatagtt gatgatctgc atttttcgat aaaaatcggc
      121 gggaagctta ctccaccgtt ttaataattg atagaaataa ctctcttctg ctttgatttt
      181 atactcgctt aatatcaatc caaaactatt cgtttttttt gaagctaatt ttttataaat
      241 atccaatgct ttggttttat cttcattgac aaagagtgtt tttacttcaa tataagaacc
      301 ctcgatattt tgtgtttctt cgataaaata gatgtttttt atatattcct tcatagtgat
      361 tcctccaaag aagtctttta tttgataagt ttactctacc gaagagttgt taataatccg
      421 tgaacaaaaa gtaattcttt ttccaatagt tgttaaaggt tttttatttt ttatagtatt
      481 ttttgttatt taagaaagga ataaggttaa acaaataaca ggttgctttt tgggtctttt
      541 tccgttcaag aaactgaacg aagaaaaggg aaagttgtct cttttgtgat aagagaaatc
      601 ataataaacg aatgacttac tttccaataa attgagaaca gtcagaacca gacaaattag
      661 ttgcatctga tgaataaagt gattattcta tgacatgagg tgatacagat gaaattagaa
      721 gtgacaaaag aagcacaaga agtattgaag aataaattag aagcagacga tcaactttta
      781 ttagatatcg aaaacggtga cggtccattt gcagaaagcc aagtcagttg ccaattggat
      841 acggcatttc gattgatcat tgtcaaaaaa gatacacaaa cagatctaag tctttattct
      901 gtagtggctg atacaccagt tggaccaatc aaaatcaaag acagtgcaat actttacatg
      961 gatgatccat ccacacttgc tttagaacca acttataata gtttacaatt aaaaggacca
     1021 agcggcttga gaaaaggcaa cttgcaagtt gttcgaaaag actagaggga taaaatggga
     1081 aaattggcga tcatcagcga cctacatgtg gacatcaata aattcggcga atcagagctg
     1141 atgcagcttt ggcaagtttt gcaaaagcac aagatcactc ggcttcatct agccggagat
     1201 acggctaata aagtagaccg ttgcctagct gttgtcgaat ttttcgtcaa taaaggatta
     1261 tctacgacat ttcatttcgg caatcacgag ttggcagatg tgcccaatga acagatgatg
     1321 gaccattttc cagacccaca ttttttaaat gaacggtata ttgctttaaa tgagcagacc
     1381 gttcttttag ggatgaatgg ctggtacgac tatcagtttt ccgaattgaa agattacgag
     1441 gcaatcgtac gaatgaagcg tttgtattgg tatgatcggt tcatccagcg tgggaaaaca
     1501 gatctcgagg tcaatgcaga aatgcttgca gctgcaaaaa atttgctaga ccagctggaa
     1561 gcaaaaaaac ttgaggtgat tttatccact catttcgttc ctaaacgggc atttatcatg
     1621 tatcaatctg ctccttatga gcgttggaac aaattaaatg catttttagg atcagaatca
     1681 ttcggagaat tacttgaccg ttattcaaat atcaaacaag tagtttttgg ccatacgcat
     1741 cgtcgctttg aagataaatc catcggtcac acgatttaca gctgcagacc ttttggttat
     1801 tattatgaat ggcagctgac ccgagatttt gtcttgaaag aacagttgct agacagctat
     1861 gatcctatga aattacgtgt cctcttgcgt gcccatcacc cagcttttca ggcctatcaa
     1921 acacaacatt tgcaagagga atttgaacaa gcgatgacaa tcgttccgta ttgagacagc
     1981 attagtaatg tgtaaaagaa aataccttgt aaaacataag gataagtaag agcagtagat
     2041 ccatttatcg tggatatgct gctttttttg cggaaaataa tcagcgaatg tttatttgat
     2101 cggatgattt gtaactcgca gagtactaga tgcaagcgtg tattcttttt ttgaacgata
     2161 atcacaaaaa aaaatataat atacattttt tatttaatta taattaatat tgattgaagt
     2221 ctaggcagtc aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc
     2281 tatgacagca gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa
     2341 atcgggctga taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc
     2401 ttttatttca taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata
     2461 tgtttagtaa ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca
     2521 gcaatgatgt tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac
     2581 ccaaaggaat ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact
     2641 cctcctgttg atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca
     2701 agccctggaa caggaggggc attatcgatt gattatggat caaaattcaa atttgggact
     2761 caaaaaattt ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg
     2821 tctagaaaac cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa
     2881 ttaagtgtct ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca
     2941 cagctgaaat ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa
     3001 acggtgaatt ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct
     3061 aaatcaggaa caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat
     3121 caagatgcgg tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc
     3181 actaaattag tttggacatt agaagataca cctaacaact agtttactga aaagaactgt
     3241 taaaaataaa tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc
     3301 taggctaaat ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa
     3361 gatatattga aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc
     3421 tttccgatca tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac
     3481 gaagaaagca aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa
     3541 agcagtgagg atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag
     3601 ggttcggaca aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat
     3661 cccaatatta tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata
     3721 ttggaaaatg ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt
     3781 tggtcaaata agaagattag tttactctcg gattttacat ttaaatctta tttatactta
     3841 ggaaatcaac gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga
     3901 atggctagta atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct
     3961 actcgttctg gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa
     4021 aatagtggtt cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga
     4081 catgtcgctt ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc
     4141 gtgacggtag ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg
     4201 gacgcggagc aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt
     4261 atacctgatc tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca
     4321 acaggtactt tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca
     4381 tcaacagctg atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga
     4441 aacgataaga taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag
     4501 aaaatgcaga ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc
     4561 aatggaaatc aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat
     4621 ctgacagatt attttattgc tggagatacg ttaataatcg aattggagac aactttgcag
     4681 gaagtaattc ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg
     4741 attgagcaat cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat
     4801 gtttattata aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg
     4861 aaaataggag agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg
     4921 gattcagcga atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac
     4981 agaaaaggca aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa
     5041 atatccagta gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat
     5101 gaacgggctt ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga
     5161 gattttgagc ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg
     5221 acatccgctg ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt
     5281 ttagattcta cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc
     5341 ggaacctttg aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa
     5401 gagaagaata taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt
     5461 gtcccacaga gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt
     5521 tcttgagacc aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta
     5581 acaatatatc agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga
     5641 atcaaatcga caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag
     5701 aagtaaaagc ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga
     5761 atagcgcaac caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag
     5821 acatgagcct gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa
     5881 aacctaaaag tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg
     5941 gtgtattggc tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa
     6001 ctaaggatca aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc
     6061 aacagacact gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa
     6121 tcaatgcaag aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc
     6181 aagtagttat tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg
     6241 aaaaagaagg attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg
     6301 gtcaaccgct aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg
     6361 aaaatggaaa attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg
     6421 tatttgaaaa agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg
     6481 ttacgattaa aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt
     6541 ttattttatt atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag
     6601 gttaagataa caaatagtaa atttttatta ttgtaggatg gttgggctct ttttataaca
     6661 atataaaata taccatttta ttaataaaaa tgcatactag taaatgatat aattctaata
     6721 acaggtttga tcgcgagaaa ggccaaataa aacgctctcc aaaatgttaa aaaaaagaca
     6781 ggacgatgta tattatacaa acaagaatcg ctgacaacag tgactcggat ccaggaaatc
     6841 agttttatcc ccagctagtt ggtgaatgca tggaaagtaa tatacgtaga aagagctgat
     6901 gactaatgaa ttatgagttt tttttaagca aagaagatca cagaagttat tcattactta
     6961 aatatttaga agcaagctca aatttatcag agtcaatatc gaatgttcaa gaagagttgt
     7021 cgctatcgac atttctcttg aaaaaaacga tcgataagct gaaacatgat ttacaaaagt
     7081 tcgatctaga gaaaaatttg cgagtaaccg tcagtgaatt ggatatatgt ctggaaatta
     7141 atggaaaatg ttctagcaaa gctctgttat ctcgttatat aacggattct ttatctatga
     7201 aaatggtatt ggctttcttt caggaaaaat atatctcagt agaaaatttt gctaaacagc
     7261 atcacgtgag ttattctgtg gcttataaag tattacaaag actaaaaaga aatctaaaaa
     7321 aatatcaaat tttttttgaa aagagaaagt tg
//
LOCUS       DACCQI010000126         7344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.131, whole genome shotgun sequence.
ACCESSION   DACCQI010000126 DACCQI010000000
VERSION     DACCQI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.131"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IVO76_002413"
     CDS             140..460
                     /locus_tag="IVO76_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430350.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IVO76_002414"
     CDS             912..1112
                     /locus_tag="IVO76_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7430351.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="IVO76_002415"
     CDS             1259..4048
                     /locus_tag="IVO76_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7430352.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKMPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="IVO76_002416"
     CDS             4095..4622
                     /locus_tag="IVO76_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7430353.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="IVO76_002417"
     CDS             4643..5833
                     /locus_tag="IVO76_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7430354.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="IVO76_002418"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="IVO76_002418"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7430355.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
BASE COUNT         2438 a         1340 c         1577 g         1989 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatgcca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaacggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactg caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ctatcatcat
     5341 caacggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtgggaaa
     5401 tcctgtgatc atcaatggaa tgatcgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggcgag caagatacag tggaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatattcg cttgtggcag aactgccggg
     5581 tgtttctctt gaaaagccac aaggagcttt ttatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctaaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcgc atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatat gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttatgatga
     6721 tgcgatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtgat
     6901 ctgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacagactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggtgatg aaaaagctgt tctgcatcaa
     7201 gtaattagaa cagccaagca aaaacagctg ttcatgtttt tgaattggcg ggacttatta
     7261 ccgcagatgt aagcttttga ataccgttta gtggagaggt tgtgaaaaag ctgtttagtt
     7321 tcgagtatta agaaaaattt tgtg
//
LOCUS       DACCQI010000127         7337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCQI010000127 DACCQI010000000
VERSION     DACCQI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.132"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..722)
                     /locus_tag="IVO76_002419"
     CDS             complement(<1..722)
                     /locus_tag="IVO76_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7430356.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="IVO76_002420"
     CDS             complement(727..1494)
                     /locus_tag="IVO76_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7430357.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1983..2408
                     /gene="lacA"
                     /locus_tag="IVO76_002421"
     CDS             1983..2408
                     /gene="lacA"
                     /locus_tag="IVO76_002421"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP7430358.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2425..2940
                     /gene="lacB"
                     /locus_tag="IVO76_002422"
     CDS             2425..2940
                     /gene="lacB"
                     /locus_tag="IVO76_002422"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP7430359.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2951..3883
                     /gene="lacC"
                     /locus_tag="IVO76_002423"
     CDS             2951..3883
                     /gene="lacC"
                     /locus_tag="IVO76_002423"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP7430360.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3886..4866
                     /gene="lacD"
                     /locus_tag="IVO76_002424"
     CDS             3886..4866
                     /gene="lacD"
                     /locus_tag="IVO76_002424"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7430361.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4894..5211
                     /locus_tag="IVO76_002425"
     CDS             4894..5211
                     /locus_tag="IVO76_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7430362.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5211..6917
                     /locus_tag="IVO76_002426"
     CDS             5211..6917
                     /locus_tag="IVO76_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7430363.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7059..>7337
                     /locus_tag="IVO76_002427"
     CDS             7059..>7337
                     /locus_tag="IVO76_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7430364.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGV"
BASE COUNT         2298 a         1252 c         1476 g         2311 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaacaaaaa ataaacaaaa aagtgttgct
     1861 ttttgtttga atgattgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     1921 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     1981 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     2041 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     2101 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     2161 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     2221 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     2281 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     2341 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     2401 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     2461 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     2521 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     2581 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     2641 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     2701 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     2761 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     2821 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     2881 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     2941 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     3001 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     3061 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     3121 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     3181 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     3241 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     3301 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     3361 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     3421 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     3481 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     3541 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     3601 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     3661 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     3721 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     3781 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     3841 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     3901 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     3961 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     4021 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     4081 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     4141 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     4201 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     4261 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     4321 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     4381 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     4441 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     4501 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     4561 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     4621 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     4681 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     4741 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     4801 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     4861 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     4921 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     4981 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     5041 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     5101 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     5161 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     5221 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     5281 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     5341 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     5401 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     5461 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     5521 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     5581 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     5641 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     5701 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     5761 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     5821 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     5881 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     5941 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     6001 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     6061 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     6121 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     6181 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     6241 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     6301 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     6361 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     6421 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     6481 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     6541 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     6601 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     6661 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     6721 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccgcaggtcg
     6781 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     6841 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     6901 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     6961 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     7021 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     7081 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     7141 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     7201 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     7261 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     7321 taaatgaaaa aggtgtt
//
LOCUS       DACCQI010000128         7210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCQI010000128 DACCQI010000000
VERSION     DACCQI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.133"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            213..1046
                     /locus_tag="IVO76_002428"
     CDS             213..1046
                     /locus_tag="IVO76_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7430365.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            1080..1919
                     /locus_tag="IVO76_002429"
     CDS             1080..1919
                     /locus_tag="IVO76_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7430366.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            1945..2505
                     /locus_tag="IVO76_002430"
     CDS             1945..2505
                     /locus_tag="IVO76_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7430367.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            2704..4425
                     /locus_tag="IVO76_002431"
     CDS             2704..4425
                     /locus_tag="IVO76_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7430368.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            4590..5732
                     /locus_tag="IVO76_002432"
     CDS             4590..5732
                     /locus_tag="IVO76_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7430369.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            5748..6959
                     /gene="thiI"
                     /locus_tag="IVO76_002433"
     CDS             5748..6959
                     /gene="thiI"
                     /locus_tag="IVO76_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7430370.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         2542 a         1207 c         1517 g         1944 t
ORIGIN      
        1 ataattttgc atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt
       61 gaagaataac gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga
      121 ctcaaccttg acctcctaat acaatggagt atctggttga gtttctttta caagaaagca
      181 gccaaggaaa tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta
      241 aagaacatga caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc
      301 tacccttagt ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga
      361 attggataag tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca
      421 gcggtcaagg agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt
      481 tagatgttat gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc
      541 cctcatcatt caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt
      601 ttgagggtta tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc
      661 ttgaacgtac aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc
      721 cacagttgct gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc
      781 tttttcgatt ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta
      841 aaaaaagaac gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca
      901 aagtagttga ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg
      961 atgcggctga aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca
     1021 atccaaccca aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta
     1081 tgatcaaagc tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt
     1141 tggaatcaac aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag
     1201 caacggggcg cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt
     1261 atgtcgtgta taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt
     1321 ttgaacaaaa agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg
     1381 tttttggtgc gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt
     1441 ttatcaagcg actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa
     1501 tcctccaggt ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa
     1561 aagaaccaat ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac
     1621 atcgctttcc cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa
     1681 aaggtggttc aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg
     1741 aagaaattat ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa
     1801 ttggagtggc catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc
     1861 aatcaaatga agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt
     1921 caatgaaaga aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca
     1981 ttccacaaaa cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa
     2041 gctcatacac gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca
     2101 cctaataaag aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca
     2161 gaagaaaaaa tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat
     2221 gcactgacag atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct
     2281 gatcgaatat tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa
     2341 cctcattatg accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat
     2401 gctccagaac ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag
     2461 aagaaatccg ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat
     2521 ttttgttatc tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg
     2581 aaaatagtat gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat
     2641 ttatgccaag cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca
     2701 taaatgggaa ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc
     2761 gtctttttgg ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa
     2821 aaacgcaagg aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa
     2881 atgcatctgg ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac
     2941 atatctactt cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat
     3001 gaaagttttc ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac
     3061 caaatggaga aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa
     3121 gaacgcaact cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa
     3181 gtcagagaag acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa
     3241 gtgatcgaaa aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa
     3301 gcccgcgagg ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa
     3361 aaaattccac cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc
     3421 agtgatactt acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct
     3481 gaagatatcg caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca
     3541 gaagttgaaa cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat
     3601 gatatcttag aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt
     3661 gcagagtata tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac
     3721 acatctcaaa gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc
     3781 gaagtggaag aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa
     3841 attccttatt cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc
     3901 gaaaagcaac aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa
     3961 gcgcaagaaa agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa
     4021 aaacaacgtt tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac
     4081 cgtattgaag aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat
     4141 cgtttagttg ctctatgtga agaagatcta gaattattag ataagaagac acatgattta
     4201 gtagatgcag cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca
     4261 cacgaagaag tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac
     4321 tatcaagaag cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc
     4381 aaacgcatcg aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt
     4441 aaaccagcaa gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa
     4501 attgaaacaa gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag
     4561 aaaagaattt caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc
     4621 caatctttcc gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc
     4681 catcaagctt gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc
     4741 agatcgctga tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag
     4801 aaggagataa ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata
     4861 tgatcatttc aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat
     4921 taggaatcga agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat
     4981 tgaaagaatt aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag
     5041 taggatcgat tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc
     5101 atttccatgt agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag
     5161 aaagagttga ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt
     5221 tcatttattg gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa
     5281 gaaatcagcg aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac
     5341 gtttatcatt agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc
     5401 taatcaatgc gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg
     5461 caccgcatat tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct
     5521 tagaagaaaa acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg
     5581 caagcagtac attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa
     5641 tcagcttaga tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc
     5701 agctttataa gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg
     5761 aaattatggt ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca
     5821 tgcaactagc acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg
     5881 ctgatcggga ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa
     5941 aactagcaaa aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg
     6001 tcgaagtttt gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa
     6061 catttaaaat tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga
     6121 atcaaacatt aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc
     6181 aaccagatat tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa
     6241 cgatcaaggg agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt
     6301 ctggggggat tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg
     6361 aagctgttca ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag
     6421 atctaacagc caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta
     6481 ccgagattca agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc
     6541 gtcgaatgat gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt
     6601 tgaatggtga atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca
     6661 atgaagtaac gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca
     6721 ttgaagtagc agaacagatc gatacattta atctttctat ccagcctttt gaagattgct
     6781 gtacgatttt tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat
     6841 atgaagagcg tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg
     6901 aagagataca agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag
     6961 aggttatgaa aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc
     7021 atgtatttta ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa
     7081 gaagttatgg aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat
     7141 caagctacag ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg
     7201 acagaagtgg
//
LOCUS       DACCQI010000129         7194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCQI010000129 DACCQI010000000
VERSION     DACCQI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.134"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..117
                     /locus_tag="IVO76_002434"
     CDS             <1..117
                     /locus_tag="IVO76_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HAP7430371.1"
                     /translation="AKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            419..1138
                     /gene="atpB"
                     /locus_tag="IVO76_002435"
     CDS             419..1138
                     /gene="atpB"
                     /locus_tag="IVO76_002435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7430372.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            1191..1406
                     /gene="atpE"
                     /locus_tag="IVO76_002436"
     CDS             1191..1406
                     /gene="atpE"
                     /locus_tag="IVO76_002436"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7430373.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            1497..2021
                     /gene="atpF"
                     /locus_tag="IVO76_002437"
     CDS             1497..2021
                     /gene="atpF"
                     /locus_tag="IVO76_002437"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7430374.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            2008..2550
                     /locus_tag="IVO76_002438"
     CDS             2008..2550
                     /locus_tag="IVO76_002438"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7430375.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            2573..4129
                     /locus_tag="IVO76_002439"
     CDS             2573..4129
                     /locus_tag="IVO76_002439"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7430376.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            4143..5045
                     /locus_tag="IVO76_002440"
     CDS             4143..5045
                     /locus_tag="IVO76_002440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7430377.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            5068..6474
                     /gene="atpD"
                     /locus_tag="IVO76_002441"
     CDS             5068..6474
                     /gene="atpD"
                     /locus_tag="IVO76_002441"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7430378.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            6488..6910
                     /locus_tag="IVO76_002442"
     CDS             6488..6910
                     /locus_tag="IVO76_002442"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7430379.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         2339 a         1284 c         1545 g         2026 t
ORIGIN      
        1 gccaaggtat tgattggtct ggcgaaaggg aaaaaatctt atgataaacg ggaagatttg
       61 aaacgaaaag atatcgatcg acaaattgat cgaacattaa aaaatttctc tagataattt
      121 cattatcctt tttcgcatat tcggtgcttt tgcttttact taatgataaa atttgttagc
      181 atttaatctg ataaaacatg attatcatag gttttttctt agatcaggag caccgtgtat
      241 aaagaaaaat tcgcttcgtt tttgactgaa ataaaaaagt gtctaatttt catgaaaatg
      301 ttttgaattt tctgtttcat ggtggtatga taccgtgtgg ttagaaaccg attggaaaaa
      361 accagatacg cacatgaatt gattttatgt gaatttcggg gaaaagaggt gaaatggatt
      421 ggatgaacgc tcgctaacgt tccacattgg accaatttgg tttgatggta cagtttgtat
      481 aatggtgtta ctgacctgtt tgattgtttt cttcttagct tactttttta caagaaatct
      541 taagttgaaa ccaacaggca aacaaaatgc gatagaatgg gtcattgatt ttagtcgagg
      601 aatcgtaaca gataatctgc ctagaaaaga attgagcaat ttccatttgt tggcatttac
      661 attgttttta tttgtttttg ttgcaaacaa catcggttta gtaacaaaaa tcgttttacc
      721 taacgaaaca acactttgga aaagcccgac agcagacccg tttgttactt taactttggc
      781 atttataatg atcttactta ctcacttatt tggggtgaaa aagttaggct tcaaaggata
      841 tttcgtaaat tcgttcttga aaccatatag ttttatgttt ccgatgaaac tgattgaaga
      901 atttacaaac ttattaacac ttgctttacg tctttatggg aatatttatg cgggggaagt
      961 tttgctaacc ttgattgcaa aaatggtaat aagcttaggc tggttctcat tgccgttagc
     1021 aattccatta gagatggtct ggatcgcatt ctccttgttt atcggaagca tccaagcatt
     1081 tgtcttcgtt actttatcta tggtttatat gagccataag attgaagtgg aagaataata
     1141 ttcgttttaa aaacaaacta ctatttatct aatttaggag gaaaacaatt atgaattata
     1201 tcgcagcagc aatcgcaatc atgggagcag caatcggagc cggttatggt aacggtcaag
     1261 ttatctctaa aacaatcgaa tcaatggctc gccaacctga aatgtctggt caattccgta
     1321 caacaatgtt tatcggggta gctctagtcg aagctgttcc tatcttaggg gtagttgtcg
     1381 ccttgattct agtttttggt gtttaattga atcagtatta aggcagaaac aatttggttt
     1441 gttactgctt ttttacatga aaactagaag tacttcaaag aaaggaaggc tcgcctatgc
     1501 tgaatcaatt ggcaattgca gaagttggca atgcgatgtt aggaaatgtc atcgttgtca
     1561 gcggctcatt tttgatacta ctggctctct tgaagcattt tgcttgggga ccaatcagcg
     1621 atattttgaa aaaacgtgaa gacaagatcg ccaatgattt ggattctgca gaacaatctc
     1681 gcatcaactc agcgaaaatg gaacaagaac gcgaacaaca attgttagcc tctcgttctg
     1741 atgcagctga tatcatcaaa aatgcgaaag aaagtggaga attaagccgc caaaatattt
     1801 tgaaggatgc tcaagaagaa gcagctcgtc taaaaagtaa agcccaagct gatatcactg
     1861 tagaacgtga ttcagcgctg aactctgtaa aagacgatgt tgcagaacta tctcttcaaa
     1921 tcgcggaaaa aatcttgaac aaagaattat ctccagaaat gcacgaatca ttaatcaatc
     1981 aatatatcga aggtctaggt tcttcaaatg aaactagata aatatacagt aggaagacgt
     2041 tacgggaaag ctttatttga actggcgatc gattcaaatc aagcagaaga aatctatcaa
     2101 gatctcgtaa gcttacgtca aatctatgaa caagtacctg gattaggtaa tatgttaagt
     2161 gatgttcgtc tagaacctca tgaaaaacga gaaatcatgg acaagcttgt tagtggctat
     2221 gatggaatcg ttaaaaactt tttagaagtt gtttatgcgt ataaccgaat ggatgatctt
     2281 ttgttcatga tcgaagaata tgaacgtcga tatgatgaac atcaaggttt gctgttggga
     2341 agtgtcacaa cagcagttcc actttcagac gaacaacgaa tacaattaga aaagaatgtt
     2401 gcaaaaacaa tgaactatca atcagtcgaa ttaaaacaaa tcgttgattc ttctattatt
     2461 ggtggcgcga ttgttgaagc taaccatcga gtaatcgacg gaagcattcg cacgcaatta
     2521 gaaaatatgc gtaatcaatt aaatagatag tacaaagagg taggtgaatc gaatggccat
     2581 caaagcagaa gaaattagtg ccttgattaa agaacaaatt gaaaattatc aaaacgtgct
     2641 ttcagtcgaa gaaatcggta ccgtaactta tgtaggtgac gggatcgctc gtgcacatgg
     2701 tttagaaaac gcgatgagtg gtgagttgct tgaattttca aatggctcat acggaatggc
     2761 gcaaaactta gaatcaaatg atgtaggtat cattatcctt ggcgatttcg agtccattcg
     2821 tgaaggagat aaagtaaaac gaacaggaaa gatcatggaa gttccagttg gagaggcctt
     2881 gatcggacgg gtagttaatc cgctaggtca gccaatcgac ggactaggtg aaatcgttac
     2941 tgacaaagct cgtccagtag aagcaatggc accaggcgtt atgcaacgta aatctgttaa
     3001 cgaaccaatg caaacaggtc taaaagcaat cgacgctctt gtaccaatcg gacgtggcca
     3061 acgtgaatta gttatcggtg accgtaaaac agggaaaact tctattgcga tcgatacgat
     3121 catcaaccaa aaaggtcaag atatgatctg tatctatgta gcaatcggac aaaaagattc
     3181 tacagttcgt acacaagttg aaacattgaa aaaatatggc gcaatggatt acacaatcgt
     3241 tgtgaatgcc ggtgcgtctc aaccagcgcc gttgctttat atcgcaccat atgctggtac
     3301 tgcaatgggt gaagagttca tgtacaacgg taaacatgtc ttgatcattt ttgatgatct
     3361 ttcaaaacaa gctgttgctt atcgtgaact ttccttgcta cttcgccgtc caccaggtcg
     3421 tgaagcttat ccaggggatg ttttctactt acactcacgc ttattggaac gtgcagcaaa
     3481 attaagtgat gatttaggtg gcggttcaat gactgcctta ccatttgttg aaacacaagc
     3541 tggagatatc tctgcttata tcccaacaaa cgttatttcg atcactgatg gacaaatctt
     3601 cttagaaagc gacttgttct acgcaggtac tcgtccagcg gttgatgccg gactatctgt
     3661 atctcgtgta gggggttctg cgcaaatcaa agcaatgaaa aaagttgctg ggactttacg
     3721 tctagactta gcaagttacc gtgaattaga agcatttaca caattcggtt ctgacttgga
     3781 tgcagcaaca caagcaaaac taaatcgtgg acgccgtaca gtagaaatct tgaaacaaaa
     3841 attgcatgca ccgctagctg ttgaaaaaca agtagtgatt ttgtatgctt tgacacatgg
     3901 attcttagat agtatccctg tagacagtat tttagacttc gaacatgaat tattcgaata
     3961 tctggatacc aatcattcag atatctttga aacaatccgt acaacaaaag atcttccaga
     4021 agaagaagcg ttgaacagtg cgattcagga atataaagac atgttcttag ctacaaaagg
     4081 aaataattcc tctacagaag ataaattgaa atccattcaa aatgcatagt gaggtgagat
     4141 gaatgggtgc ttcattaaat gaaatcaaga cacgtatcgc ttcaacgaaa aaaacgagcc
     4201 aaatcacgcg ggcaatgcag atggtgtctg cttctaagtt gacaaaatct gaagcttctt
     4261 cacagaagtt tcaggtttat gcaacaaaag taagagaaat cgttacgcat ttgactgcta
     4321 ctcagttaaa tgatattgcc tcagaaaatc ctagagggga tatcaattac aacagcatgc
     4381 tgctcagccg tcctgttaag aaaactggtt atatcgtcat cactgcagat ggcggtttag
     4441 tcggtggata taacagctcg atattgaaac aaacgatgtc tattttagaa gaagaccacc
     4501 aatcacccga tgattacgtg atgatcgcaa ttggcggaac tggtgcagac ttctttaaag
     4561 cacgcggtat caatctagca tatgagctgc gcaatctatc tgatcagcca agctttgatg
     4621 aagtccgcaa aatcgtaaat atggcaacaa ctatgtacca aaacgaagta ttcgatgaat
     4681 tatacgtatg ttataaccat catatcaatt cgctaactag tcagtttcgt gtagaaaaaa
     4741 tgctgccaat ctcagactta gatccagaag aagcaactac tttcgaacaa gaatatattt
     4801 ttgaaccttc aaaagaagaa atcttgtctc agttattgcc tcagtatgct gaaagtttga
     4861 tctacggtgc gatcgttgac gccaaaacag cagaacatgc tgctggtatg acagcaatga
     4921 aaacagcaac tgataatgct gctaatatca ttgatgagct gacggtttct tataaccgtg
     4981 caagacaagg ggctattacc caagaaatta ccgaaattgt tgccggagct tcggcactag
     5041 aatagtattg agattggagg aaaaaagatg agttcaggca agattgttca agtaatcggt
     5101 cccgttgtcg acgtggaatt ttctttagac caatccttac cagatatcaa caacgcatta
     5161 gttgtttata aaaatgacga aaataaatca aaagttgttc ttgaagcagc gttagaatta
     5221 ggggacggag tgatccggac cattgcgatg gaatcaacag atggtttaca aagaggaatg
     5281 gaagtcattg atacaggcaa agcaatctct gttcctgtag gtaaagaaac attaggtcgc
     5341 gtattcaacg tactaggaga tacgatcgat ttagaaacac ctttcccaga agatgcggaa
     5401 agaagcgaaa ttcataaaaa agcaccagcc tttgacgaat taagtacaag tacagaaatt
     5461 ttggaaacag ggatcaaagt tatcgatttg cttgccccat atttaaaagg tgggaaagtc
     5521 ggactattcg gtggtgccgg tgttggtaaa accgtactga tccaagaact gatccataat
     5581 atcgcccaag aacatggtgg tatttctgta tttactggtg taggtgaacg tactcgtgaa
     5641 ggtaatgact tgtattatga aatgaaagat tcaggagtta tcgaaaaaac agccatggtg
     5701 ttcggacaaa tgaacgaacc accaggtgca cgtatgcgtg ttgctttgac tggtttgacg
     5761 attgcggaat atttccgtga tgtagaaggt caagatgtac tgttgtttat cgacaacatt
     5821 ttccgtttca ctcaagctgg atctgaagta tcagccttgt taggacggat gccttctgcg
     5881 gttggttatc aaccaacatt ggcaacagaa atgggtcaat tgcaagaacg tatcacatct
     5941 acgaaaaaag gttcgatcac atcgatccaa gcaatctatg tgccagccga tgactacact
     6001 gacccggcgc cggcaacagc attcgcccat ttggatgcga caaccaactt ggaacgtaaa
     6061 ttaacagaac aagggattta tccagcggta gatccattgg cttcttcttc aagtgcttta
     6121 gcacctgaaa tcgtgggaga agaacattat aaagtagcaa cagaagtcca acacgtattg
     6181 caacgatatc gtgaattaca agatatcatt gctatcttag gtatggatga attatccgat
     6241 caagaaaaag tgttagtttc tcgtgcccgc agagtccaat tcttcttgtc acaaaacttt
     6301 aacgtggctg aacaatttac tggtcttcca ggttcatatg tccctgtaga agaaacagtg
     6361 aaaggattcc gcgaaatctt ggaaggcaaa tacgatgatc ttcctgaaga agctttccga
     6421 agtgttggcc gaatcgaaga cgtcgtagaa aaagcgaaaa cactaggcta ctagaaaggg
     6481 gtgccccatg gatcaatatt taacagtaaa tgtggtaact cctgccggtt tggtctatga
     6541 tcaccatgca gctatcgtcg ttgcacgtac gacagctggt gagcttggga ttttgcctaa
     6601 acatgcacca atcatcgttc cattgacgat tgatgaagtt cgtgtaaaac gaacagattc
     6661 agatacccac gtcgattgga tcgctgttaa tggcggaatc atggaagttc gtgataatgt
     6721 tgtttcaatc gttgctgata gtgcggaacg tgaacgtgat atcgatgtat ctcgtgcgga
     6781 acgtgcaaaa caacgcgcag aacgccaaat cgaagaagca aaagcaaaac aagatacgaa
     6841 cgaattgaaa cgggcaactg ttgcgttgca ccgagcaatc aatcgtatca aagtatctaa
     6901 gcactcataa aatgagatgc ggatgtttat ccgtgtttaa gaacagtaaa aagaggttat
     6961 gacagaagtg gtcagcttca agacataaga aggaatttac gaaaattgct tctcaaattt
     7021 ttgtgaaatc tcagcttatt tttcgaagaa gctacttctg gaacaccgta tatcagaggt
     7081 tgtaaaatca gcgttttgtc ctgagtatta gagaaacaaa tggaaaatag ttcctcatat
     7141 tttgccgttt tttgagctaa taccgagggt cagatgattg aacaccgttt attt
//
LOCUS       DACCQI010000130         6907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCQI010000130 DACCQI010000000
VERSION     DACCQI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.135"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1591)
                     /locus_tag="IVO76_002443"
     CDS             complement(<1..1591)
                     /locus_tag="IVO76_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7430380.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIAMIPLPLLAITSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYLKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVKHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAH"
     gene            complement(1733..1957)
                     /locus_tag="IVO76_002444"
     CDS             complement(1733..1957)
                     /locus_tag="IVO76_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7430381.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2208..3794
                     /locus_tag="IVO76_002445"
     CDS             2208..3794
                     /locus_tag="IVO76_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7430382.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            complement(3860..5635)
                     /locus_tag="IVO76_002446"
     CDS             complement(3860..5635)
                     /locus_tag="IVO76_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7430383.1"
                     /translation="MLNTLKPLINQLTKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHFSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5848..6750)
                     /locus_tag="IVO76_002447"
     CDS             complement(5848..6750)
                     /locus_tag="IVO76_002447"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7430384.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTAAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
BASE COUNT         2034 a         1371 c         1256 g         2246 t
ORIGIN      
        1 ggtgagctgc aatgatagtt gttttctctt ttcttatttc ttttaaatta tgcaagatag
       61 cttcttctgt tttggcatct actgcagaaa gtgcatcatc taacattaat aattcaggat
      121 ttttgatcaa tgctcgagca atcgaaatcc tttgtttctg tccgccagat aaagagactc
      181 cacgttctcc aactaaggta tcataacctt ttggtaattc cttgatttca ttgttgataa
      241 atgctaaaga agccgcatgc tttacttctt cttctgaaag ttttggatta gaaaaacgga
      301 tattatctcg aatggtcatc gaaaacaaga agtgatcctg aggcacatag ccgattgatc
      361 ctaataaagc attcaatgaa tattctttga tcgaacgtcc gccaaattgg attttccctt
      421 gataatcatc gtattcacgc atcagtaatt tgatgatcgt cgtttttccc gcaccagttt
      481 tcccaacaat acctaatgtt tctccttctt ggattgcaaa cttgatttgt tctaatgctg
      541 gtgattcttc tttcagataa ttaaaagagg aaatagctac attcagctcg ccttttgccg
      601 gggtctgcac tgcacccagt tgttccacga tatgcgtttt ttcatgcaaa agttcattga
      661 cgcgatcata agaagcattt cctctttcca atacgttgaa caatcgacca atcgcaaaca
      721 tcggccaaac aagcataccg atataactaa taaatgagac catctgcccg atcgagatcg
      781 tgccttccaa gacatacttt ccacctaaaa taattgttgc aacataagac agaccgataa
      841 tcaaggtaat gaatggatca aataaggagt ctaaaaaatt gactcgtttg tttttaataa
      901 ttgcatcttc aatcttttct tcaaaatccg ctagatcttc tttttcttgt ccaaatgttt
      961 taatgacttt gatcccagtg atactttctt gtgtcttatc attgatacta gaaaaagctt
     1021 cttgcgaatc acgaaaagca tcatggagtt tcgatcccag cactcgtgag gtgatggcca
     1081 gcaaaggcag aggaatcatc gcaatcagtg ttaagcgcca atcaataaac aaaatcatcg
     1141 caatgatcgt tgttccgccc gtaatcactg aatctgcaaa ggtcaagata ccagctcctg
     1201 ctacattctg gatcgcattc aaatcattcg ttgcatgagc catcaaatcc ccagtccgat
     1261 atttttggta aaagagattg tccatttctg taaaatgttt gaataactgc tgccgaagtt
     1321 ctttctccaa ccgagctgca cttccccaaa tattcgtccg ccagatataa cgaaaaagat
     1381 attgtaaaaa agcagcgata atcaatagga agatccaccc gaaaatgatc tttattgata
     1441 aatttttatc ggcaatctca tcaatgatga tcccaatcac ttttggagga actaactgta
     1501 ccaaggccac tgcgaatagc gagaaaactc caattaaata atgtttcttt tcctgacgaa
     1561 aaaaccagcc taattttcta aaaatcgaca ttttactcct cctttgtctt tatcaagagc
     1621 ctaggattgt ctgtgcttgt cttttttgct gaataatctg aatagtctaa caaattacga
     1681 aataaaaaac gggctgaaaa aatcagcccg taaaaagtcg ttgggtcaga acctattttt
     1741 tcccttggtt cttcatttgc tgcatcatct ggcgaatttt cttctcagat ggtttttgcc
     1801 ccattgacat catcattgat cgcaacatgt cctcattaac gggaggattt ttttctaaat
     1861 agtctttcat atatttacgc gctaggaaaa agccacctac tgcgccgatt agcaaagcaa
     1921 taatagcaat aagtacgaca atacctgttg ataacatata aatctctcct ttctatttcc
     1981 gaattgctct caatgactta ttttactaaa gtttattgaa aaaagaaagg cgtattcttt
     2041 agtttcatgc aaaaaatagc ggttttttta gaggagaaca aaatttcttt cgtcatctag
     2101 caagacacgc cacgaaaata aattaggcat accgaatctt tttctttaat tttttctttt
     2161 tgaggtgtag aatgtaatcg tagataaata ggaaaggtgg ttttttcttg aataatcaat
     2221 ccaataaaca atttcataag catcgactta ttgaacatac aaatggttta tctttagctg
     2281 aagtaaatgg aacagttgat gtaccaaagg ggaaaagttt ttttaaaaca cttcttgctt
     2341 actctggacc tggtgcgtta gtagcagttg ggtatatgga tccgggtaac tggtctactt
     2401 cgatcaccgg aggacaaaac ttccaatatt tattgatgtc agttattttg atgtctagtc
     2461 ttattgctat gcttttacag tatatggcag caaaattagg aatcgtgagc caaatggatt
     2521 tggcacaggc aatccgagca cgcacaagta agacattagg tgtcatttta tggatcttga
     2581 ctgaattagc cattatggca acagatattg cagaagtaat tggtgcagca atcgccctct
     2641 acttactttt tgatattcca ttggtgattg ccgtctttat tactgtatta gatgttttcg
     2701 ttctcttact actgacaaaa gtcgggtttc gtaagatcga agcactggtt gtctgcttaa
     2761 ttttcgtcat tttattcgtt tttgtctatc aagttggctt gtctaaccca gattggcaca
     2821 gtttatttgc tggctttatc cctaacagcc atacattctc agactcacct gtcattggtg
     2881 gcgaaactcc gctaactgga gctttaggga ttattggtgc aacagtcatg ccgcataact
     2941 tatatcttca ttcagcagtt tcacaaactc ggaagatcga tcaccaaaac gaagaagatg
     3001 tagcacaagc tgtacgtttt tctacttggg attctaatat ccagttgtca atggcttttc
     3061 tagtcaatgc actgctattg atcatgggtg tcgctgtctt caaaaccggt tctgtccaag
     3121 atccttcctt ttttggtctg tatgaagctt tatctgatcc atcagctatg agcaatggta
     3181 ttttgattgc tgtagcaaaa tctggtatct tgtccacttt atttgctgta gcacttcttg
     3241 cttctggcca gaactctact attactggta cgttgaccgg tcaagtcatc atggaaggat
     3301 tcatccatat gcgtatcccg atttggctaa gaagactggt cacacgattg ctttctgtga
     3361 ttcctgtatt gatttgtgtt ttgtttacaa gtacaaaagg aacgatcgaa gaacataccg
     3421 ctttgaataa cttaatgaat gaatcccagg tgttcttggc ttttgcgttg ccattttcga
     3481 tgattccttt actgatgatg accaacagcc aagcggagat gggtagccga tttaaaaatt
     3541 cattcatcgt aaaacttctt ggctggttct ctgtgattag tctaacttac ttgaacttac
     3601 gaggattacc aggacagatc gaaagtttct tcggcgagca agccacttcc gctcaactgg
     3661 cattggctga tcacatcgct tatgtaatca ttgttcttgt ccttctcctg ttggcatgga
     3721 cgattggtga tttatataaa ggaaacaaaa aatatgaaca gcttttgcag gaaacggtag
     3781 aagaaatcga ataaaaactg atttttccac acaaagaccc ttgatagtta ggttaaaaat
     3841 ctagctacca agggtctttt tatttagaag ataaagcttt tttcagattt aatttactga
     3901 aaactagcat gcttgttact acgatgaatt gcgttaatgg cacgcctgca ccagtgtaga
     3961 cacttaaaaa atagtccttt ttttctggcg cataagaaca aaaccatgag acaccgaaaa
     4021 gaaaaaccaa ttgtgtcagt attaatccgg tgaccacaat caaagaggaa ctataatagg
     4081 cacgatcagc ttttgcaaat acggttccta gtatcataag taatatccct gctattgaag
     4141 caatcagcac gggaatagcc agaacttggt aaatttttat gatgacattc gcgacttttg
     4201 ctgattttgt taattggaag ttaggtgttc tcggttcatt actatcgatc caattctcca
     4261 ttagcggaag gtttaataat tcttgagcct gtttgacttg ttcttgcgtt ccgaaactat
     4321 aatttcctcc ttgatggaaa tctttatatc gccatgcata agcgtatcct gattgaagga
     4381 aatcgaatac gattggcaga tcactcatcg tctttccatc tcctgatccc atcagataga
     4441 actctttttt ctttttgatg gttcccttgt gataggcttc ttctaattct tggttgactt
     4501 ttttccaaaa atcattggtt tcttttgcat tgttttggta ataaccagat tcgcttacta
     4561 catctcgcaa tgcccaaaag atgatatctc cgggtaattc ttttactttt ccaccttgag
     4621 tccacgcgtg agtcgtatat aattcgtcaa tacgggtaga aacagtatgg aatgtaggag
     4681 atgcggctaa tgctttattt agtgcagcat gtgagaccca tatctcactg ttttctttat
     4741 tcaagttcgt ttcatcatct attcggatca gttgtttcat caaacgtcca aattctccac
     4801 tcgaacggtc attgatggtt gcaataccgt atgttgtctc atttttatat tcgatccact
     4861 gatagacgat ggaaaagcca actataggaa gtaaaaacaa tgaaagtttc agccattccc
     4921 gtttcgagag aagaaagagt cgttttttta acttctttac ttctttgaaa gaaaagtctt
     4981 gccctagcaa gatctggacg aaactgatac ccaaagctac tacagtaaaa ggaagaagcc
     5041 agatagaatc ttcgcgaatg aaccaaaaaa aaggaaaagc gacagaaagc cctaaaaacc
     5101 atggcagcaa ttttctgcta gaaccgtatt tgcggtaata aatagctaat aaacaagcga
     5161 taatgatctc aacagccgga atcacgaggg cattacgata cgttcgagtg gaatattcat
     5221 gtgtaaaact aatcggagaa aacaaaaaat aaaaatagat acttaaaaga agatacttgt
     5281 tcttcacgat cggtttaaac gcgagtgcaa ccaatatact ggcaaaaata ctgaacatac
     5341 cgaataaaac tgcataagaa aaatgcagtt tatgtgcaag tactaaaaat aagctgtagg
     5401 agattccttt ggaaagtgtt gtagtcgtat actcccctag ccatttaccg tccaaaatat
     5461 tttgagcata gcgaatcagc aactgatcat cataggttgc cgacatgtct gttccataga
     5521 ttgctttact atataaatac aaacgaataa ttgtaaataa tagggctatg ccaagaataa
     5581 agtatgtatg tttcttagtc agttggttga tcaagggttt taaagtgttt agcatctttt
     5641 cgtccttcca atcacgtttt gccacttgct tgtgaaactt ttcgattttt tacttttcta
     5701 ccagtatacc ctgtttgtct aaatttaaaa agagggggga gaagatgcat gatgaaaaag
     5761 actgcaaaaa gtactccttt gtttcttaaa caaaaaagga caaggcaatg tgcctggtcc
     5821 ttttttgctg ctgtcaaata gaaaatctta gagtatcact tcttctgcta ataactcagg
     5881 aaacacttct ggatcgaatg tttgcgcttt gaacatggcg atttcttttc catatggtgc
     5941 gtaagctttc tttttatctt tgccgacata gggagtttcc aagattttcg aaatattgtc
     6001 aaacattgga tcatggacga tcttattcaa cgtgtcgaat ccgattgttc caaaaccaat
     6061 attggcgtgc cggtctttat gcgaaccttg cggattttta gaatcattga tatggagtac
     6121 tttaagccga tctaacccga tgatcttatc aaactcttcc attacacctg aaaagtcttc
     6181 tttaatatta taaccggcat cattcgtatg acaggtgtca aaagtaacgg ataatttttc
     6241 attcaatttt actccatcga tgatttgcgc taattcctca aatgttcgac cgatttccgt
     6301 acctttacct gccatcgtct ccaaagcgat ttgtgccgtc tgttcttttg tcaaaacttc
     6361 attcaagcct tcaataattc tagcaattcc ggctgctgtt cctgccccta catgagcacc
     6421 tgggtgaagt gtaatttgag tagcgcctaa agcttccgca cgaacaatct cttctctcag
     6481 aaattggacg gcaaaaggaa acatttctgg tttgatcgta ttccccaaat taatgatata
     6541 aggggcatga acgacgatat ttgttagacc gttttctgcc atgtacgctt ttccggcatc
     6601 aatattcatc tcttcgatgg cttttcttct agtgttttga ggggcgcctg tatagatcat
     6661 aaaagtcgaa gatccataac tggctgcttc ttctgctgct cctaacagca ttttctttcc
     6721 gctcatgctg acgtgtgagc ctaataacat ggatggattc ctccttgaaa ggcaattgtt
     6781 actaacaatt cctatatatt ggtaagatga tatacaaaga aaatgattga tttttctttg
     6841 ccctgttctc catagtaact gaaattttct ctaaagaaaa gcttaagaac tcttttataa
     6901 aaaagat
//
LOCUS       DACCQI010000131         6610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCQI010000131 DACCQI010000000
VERSION     DACCQI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.137"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            810..1484
                     /locus_tag="IVO76_002448"
     CDS             810..1484
                     /locus_tag="IVO76_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7430385.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1481..1822
                     /locus_tag="IVO76_002449"
     CDS             1481..1822
                     /locus_tag="IVO76_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430386.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            1852..2211
                     /locus_tag="IVO76_002450"
     CDS             1852..2211
                     /locus_tag="IVO76_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7430387.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2414..2593
                     /locus_tag="IVO76_002451"
                     /pseudo
     CDS             2414..2593
                     /locus_tag="IVO76_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            2735..4816
                     /gene="cadA"
                     /locus_tag="IVO76_002452"
     CDS             2735..4816
                     /gene="cadA"
                     /locus_tag="IVO76_002452"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7430388.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            5127..6593
                     /locus_tag="IVO76_002453"
     CDS             5127..6593
                     /locus_tag="IVO76_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7430389.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2188 a         1075 c         1410 g         1937 t
ORIGIN      
        1 ggaagaggtt gtgcgcctgc cgttcagctc cgagtcacaa ggagtaattt caaaaaacag
       61 tttttcatgt ttgatgacta ttgtgacttg tgaccgagga gttggccgga gagcaccgtt
      121 tattctaaaa aagaactgcg aactctcgca gttctttttt taaaatacgg gataaaggaa
      181 gtttccagca gacaagagaa gaaagcaaaa gaaattgcta tttgattcac aaggttctat
      241 aacgaaatcg caatcaatca aacatttaca aagaaggtaa ttcaaatgga aatctgattt
      301 gatgatgctt ctgctgttga aataaagcag catctagcac ctgaaaacaa actaatagta
      361 acattcgaag atggagtagg tccgtatttt cagcatgcaa taatccatat gtaagttcaa
      421 ttcacccttg aaatcatcgg accagataaa gttatgacca atctatccca agcaatctag
      481 aagagatatt agtaaaggga catttactag aagtcttaga tgagcctata aatatatgtt
      541 ctgatggcgt catcagtggt ctgattgacg gaagcttagg tgtgatcgat ctctctggac
      601 caaacaacga aggaatcaag gggaatccgg cacgttaaga aaaaaatagc ataaaaaact
      661 agacacttga tgagcgttca ttcaattgtt tgaagggact aggctggtgt cattttttat
      721 acatatattt tctctttcgt cttgataggg cttatgggat aatataagga agaaaatcat
      781 cgttttattc aaaaaggagt ttttgacaaa tgaaaaaacg tatattattt atcggtgata
      841 gcattacaga cgcaggaaga gaccgagcag aagcaactga tctaggtaaa ggttatccac
      901 ttttgattgc cggaaaacta atctcccaat atccaacgat ggaatttgaa tgcatgaacc
      961 gtgggatagg aggagacaaa attgcagatc tgcaagtacg ctggcaagaa gattgtttag
     1021 ctttacaacc tgactatgta tcgattttga tcggaatcaa tgatacctgg cataatacta
     1081 gcgatcaggc tatattcgga actgagaaat ctgctcagca atttgaatcc gtttatcgaa
     1141 aattactaaa tgaacttcaa acagtgggaa tctgcaacat catcttgatg gagccgtttg
     1201 tacttcctat aaccgacgac cgtcatggct ggcgtaaaga cttagaccct aaaatccaaa
     1261 ttgttcgtaa acttgctaag gaatatggcg cattgctggt tcctttagat ggtatattca
     1321 atgctttagg gattgtttat ggtttttcga aatatacaag agaagatggg gtccacccaa
     1381 cgattggcgg acatgagatc attgcacaaa gctggataga tgcaattgcc gcagtcgatt
     1441 tttttagtgg agataaaaat gagcgaaaga aaagtgagaa atgatggaaa tacggtgggc
     1501 agtaaaagaa gatctacctc aaatcaatca gctgattact gatcatccag aatcattcgt
     1561 aaaagcaaaa acggcagctg acctaaatga taagaataaa ctattgctcg ttgctgtaac
     1621 aaaagaaaaa cggattatcg gatttgcgca agcggacgtg caacagtcag aagaacgatt
     1681 ggatcagcga ccatcaggaa aactaatcca acacacatta gtaacaaaag aaaatacgga
     1741 agaaatccaa aaaaaatttg cagaatattt gatactggaa gctaaaaaaa gaagaataaa
     1801 atcatttttt gcccagtttt aatcaaaaac agtaatagaa ggagaaatac aatgagttgt
     1861 ttaggaaatg tcatctggtt tatattcggc ggactaatcg gtggtcttag ctggttagca
     1921 gcggggatac tttggtgtat cacgatcata ggtatcccaa tcggtatgca atgttttaag
     1981 atcgcctccc tttcttttgc gccttttggc aaagaagtaa tttatcacca gtctggcatg
     2041 tcattgattg ttaatatcat ctggctgatc gtttcaggcc taccaattgc tctcgcacat
     2101 gtcgtcagcg gcatccttct atgtatcacg atcataggga taccttttgc caagcaatct
     2161 ttcaaacttg ctcgattggc gttgatgcca ttcggtgcag aaattgtata aaagagaata
     2221 ataaaggtta gataaagcat agagtcaggt gggatacatg tatttcacct gactctatgc
     2281 gttctcctgt ataagttctt ccatgtgact tttacgtctt ttatttatat ggggttactg
     2341 tttatggaaa gttgaaataa ttttcattaa agaactcttt ctatattttt gaaagcgtag
     2401 tggagcttcg aatatctggg aaggacattt tgcagggatt gaaaaaagat attgtcctat
     2461 cgaaaaatta atgaatttag acaaattgcc agcaacaggc ttcaaactta gttgctatcc
     2521 tgtcaagatc aagaaggcaa gtgcgggttg gataagagca gtggcaatga tcgaaaagaa
     2581 aaagaaagaa taagccaaaa catctgtatg gaagaaattt tccgatgcag atgtttttca
     2641 ttttttcttt cggatacctt gacatatgaa taaccgttca tttataatta gttatgaaga
     2701 gatattcata tgttgaggcg aggaggtcat taaaatgaaa agttacagat tagaaggttt
     2761 agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt cagaagatca atggagtcaa
     2821 ggaagcaacg gtaaacttca cttctggaaa actaacgatt gacgcagagg aagatcattt
     2881 ggctacgatt gaacaagaaa taaaaaaagt agtgaaggaa ttagaaccag atgtcaaagt
     2941 aacagaaata gataaggaaa aagtatctga acatggaaat gaaaaagaga gaaatacgct
     3001 gttccggatt ttattttccc ttgcaggtat cgctttattg ctattatttg attttaatga
     3061 accaattcgc ttgatagggt atttattgat ttatctgctg ataggatatg atgtagtgaa
     3121 gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat gagaattttc ttatgtctgt
     3181 tgctacgatt ggcgctatgt tgatcggtga atatccagaa gccgtagcag tgatgctatt
     3241 ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt cattcaagaa aatcgattcg
     3301 cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag acgacggaag gattgatgac
     3361 cgtgaaccct gaagacgtgc agatcggtca gtttgtactt gtgaagcctg gtgaacgtgt
     3421 accgttagat ggaacgatca tcgaaggaga atcattagtc gatacgtcag cacttacagg
     3481 agaatcagtc cctaaatctg tatatgttgg tgaaacagta ttaagtggat ttatcaataa
     3541 aaataaacca tttttagttc aagtagaaaa atcttatgaa aattctacta tcagcaaatt
     3601 attagaatta gtagaaaatg ccagcagtaa aaaagcacca gctgaaaatt tcattactaa
     3661 attcgcgcgc tattacacac cagtcgttgt aggattggcc gttttattag ctgttttacc
     3721 tccacttgtt gtttctggag cggcgttttc tgaatggatc tatcgagcat tgacattttt
     3781 agtgatctca tgtccatgtg cattagtgat ctccgtaccc cttagtttct ttggcggtat
     3841 cggaggagca agtaaaatcg gtgtattagt aaaaggttca aattacttgg agttgcttgc
     3901 acaaactgag acagtggtat tcgataaaac cggcacatta acgaaaggtg acttttcgat
     3961 ccagacgatc gatacaaaaa tcgatccaaa gatattcatg caatatgttg cgagtgcaga
     4021 acagttttct acacatccga ttgctcaatc tgttttagaa ggctacgcag gtccgctttt
     4081 accaacagct aatatccaag aatttgcagg tgaagggatc ttagctgaag ttgatggcaa
     4141 acaagtatta gcaggtaatc acaaattgat ggaaagattt gaaatctctt ttccttcaag
     4201 tcaagaaatc ggtacattgc tttatctagc gatcgatcaa agttacagtg gttatcttgt
     4261 tattgcagat accttgaaag aagatgccgt agacgcattg gttcaattaa aaaaggctgg
     4321 tgtgaagaac acagtcatgt tgacgggcga ttcgaaaaaa attgctgatc atatcgggaa
     4381 gcaagtaggc gtggacaaga tatatagcga attgcttcca gaagataaag tgcagcggtt
     4441 agaggaaatc ttacaaagaa acaataaaaa gaccgcattt gtcggagatg gaatcaacga
     4501 tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg ggtggattgg gaagcgatgc
     4561 tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa ccatcgaaga ttgcagaagc
     4621 aatccattta gcaaagaaaa cattgaaaat cgtcaaacag aacattgtct ttgctattgg
     4681 gataaaaatt ttggtacttg ccttaggtgc ttttggtttt gcatcaatgg gagatgcagt
     4741 atttgcagat gttggggtga cagtattagc tgttttgaat gccatgcgaa gcttgcatgt
     4801 aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt cctctttttt ttatatcctt
     4861 ttcttaatga aaaatgtaaa gaaaatagat aggattataa aataactaat gtttttaatt
     4921 ttgcagaaaa ttcaaaatag cttgaaattc aataaggtaa atgaaataaa agaaaataac
     4981 ttattttttt gatggaaaaa atatgaaaaa acgatgtgta ttaaataaaa gaatacacac
     5041 ataaaataac actgtcttgt aagtttgtta aaaattgaat atgattacat ctggataaca
     5101 aatttttatc ataaaggaga gaacgaatga acaacaaaca attacgctgg tttactgttg
     5161 cgttaattgc atttaacatg gtttggggca tggggaatgt agttaataac tatgctcagc
     5221 aaggagtatc agttgtcacc tcttggctat tgattttggc catttatttt attccatacg
     5281 cattgatcgt tggacaatta gggtcagcct tcaaggatag taaaggaggc gtcagttcat
     5341 gggtcgaaaa tacgacatct aataaacgac tggcatacta tgccgcttgg acttactggg
     5401 tcgtccatat tccttatttg gcacaaaagc ctcaagcaat cctgattgct ggcggttggg
     5461 cagtccaagg aaatggaaat atcgtaaata cgatgtcagt acaaatggta gcagttattt
     5521 cacttattat atttcttgcc tttttatatt tatcaacaaa aggactttct actttgaaag
     5581 tgattggcgg actggccgga acagcgatgt tcgtcatgtc gatcttattt attcttttag
     5641 ctgtaacggc accgttcatc aatccaacga tggaatttgc cacaccacat atgaatcaag
     5701 tgaaaacata tattccaaca tttgatttca gttatttcac aaccgtttcg atgctagtat
     5761 ttgcagttgg aggagcggaa aaaatatccc cttatgtcaa ctctacaaga aatcctgcaa
     5821 aggaatttcc taaagggatg atcttcttag cagcaatggt agggatttgt gctgtattag
     5881 gctcagtcgc aatgggtatg ctgtttgcta gcgataatat tcctaaagac ttgatggcaa
     5941 atggggcata cagtgctttt caaattttag gtaattacta tggtatcggt aactcattga
     6001 tgatcattta cgcattgaca aacatgattg gacaaatctc cgcattagcc ttttcaattg
     6061 atgcaccact aaaaatcttg ttagcagatg cggatccaga atttgtacca aactggctac
     6121 gtaaacgtaa tcgtaaaggt acattggtga atggttatct tctgacaggt atactagtag
     6181 gaatcattat tttgttgccg atctttggta tcaaagatat gaatgaatta gtaaaatggt
     6241 tgacgaactt gaactcagtt gtcatgccaa tgcgttacct atgggtgttc ttggcatata
     6301 tgatgttgaa taaagcttat aagaaattca attctgaata taaatttgtt aaaaatccaa
     6361 aaatcggttt tatctttggt gcttggtgct ttgccttcac tgcgtttgca tgtatcctag
     6421 gaatggtgcc aaaagtagat tatactgctg atcctaaagc atggtggttc caattattca
     6481 cgaatatcat cacaccaatt gtcttgatct tattaggtat gatcttgcca gcaattgctc
     6541 gtcgagacaa aaacaaagaa atcactgaac cagaaacagt ggttcagcct taaaatagaa
     6601 atacttgtta
//
LOCUS       DACCQI010000132         6601 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCQI010000132 DACCQI010000000
VERSION     DACCQI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.138"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..357)
                     /locus_tag="IVO76_002454"
     CDS             complement(<1..357)
                     /locus_tag="IVO76_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7430390.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKL"
     gene            complement(813..1298)
                     /locus_tag="IVO76_002455"
     CDS             complement(813..1298)
                     /locus_tag="IVO76_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430391.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1437..1652)
                     /locus_tag="IVO76_002456"
     CDS             complement(1437..1652)
                     /locus_tag="IVO76_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7430392.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            1876..2676
                     /locus_tag="IVO76_002457"
     CDS             1876..2676
                     /locus_tag="IVO76_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7430393.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            2695..4563
                     /locus_tag="IVO76_002458"
     CDS             2695..4563
                     /locus_tag="IVO76_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7430394.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4556..5047
                     /locus_tag="IVO76_002459"
     CDS             4556..5047
                     /locus_tag="IVO76_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7430395.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5035..5589
                     /gene="srlA"
                     /locus_tag="IVO76_002460"
     CDS             5035..5589
                     /gene="srlA"
                     /locus_tag="IVO76_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7430396.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5607..>6601
                     /locus_tag="IVO76_002461"
     CDS             5607..>6601
                     /locus_tag="IVO76_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7430397.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2305 a         1055 c         1302 g         1939 t
ORIGIN      
        1 caatttcagc acaccgtttt taaaggttcc agtgattttt tcttcatcaa cattaggaag
       61 atagaatgaa cgtttgtaag agaccgaatg tctttctttc cgtacatagt tgccatcttc
      121 tttatcttct gtatgggact catgattggc ggagatcgtc aaagtattat ctgaatattc
      181 aactgtcgtg tcttctttgt cacagccagg catatcagca gttagtttgt attctttttc
      241 attttcgact aaatctactt ttggataact tgaaacacct aaaaaatcat tgaatgctgg
      301 actaaacacg tcattgaaat cagggaacat gtcgcgaatg ctaggtacat ttgccatttt
      361 tgatcatcct ttcaataagt attttttatt tatccctatt tacgtaataa ctctattttg
      421 aaaaaaatgc aagtataatg gttagctttg tgaatatttc atctaatacg atacaaaggc
      481 tatctttcta atttgaaaaa gggatcatta aaataataag aagaaaatat gtcaaaaggt
      541 aaaaaagtaa attcttctat ccaaatatag aaaaattgaa tctagaaaac cgcccgacaa
      601 taaatttcat taactcagtt gaccgaaaat gaagtagata ccaaaaattt atcaagagag
      661 caagtagaga agtgagtata ttcataagag aaatagctgt aacgaattat taggttaatg
      721 caaatgctta tttatactcc gtataacttt ctcagaaaaa atgataccaa taatgacttg
      781 ctagtactgt aaatccgtat aatatcatct aatcaaaatg gtgttccatt tgcatctgtc
      841 atcgtaatcg atccttcggg tgacactcga taaaaaccaa cgcttccaga tcctccttgg
      901 gaacggattt gtttgctgga agctgtgaac aaataatctc gatcaatttt ttgataaaag
      961 ctaataacaa tatcgtcact ttgtaattcg gaacggtggg caataagaaa tagaagtgct
     1021 ttgtcagagt tttctaatgg aagagaaggt acagtgcttt cttgcatttc agatgttgaa
     1081 gaaatgctaa acgactgaat acttgattgg ctatgttgcg aatctacatt accagtagaa
     1141 gtattagtaa tggaagtagt agaagtactt ttagttgaac caacctcagt tgttgaaatg
     1201 gctgctgaag attctttaac agaattcttt gttttatatc cacaggcaga aaaaagtatt
     1261 acagttccca acaagcctgc gtaaatccct ttatgcatga tagctcctcc taaaatagat
     1321 tatctttatt cagtatatca cttaaacaaa gaaaagcaga tggtttaaaa tgaaaaaacc
     1381 ttcccaataa agggaaggtt catgaagata ataacttttt taataattat agctttctaa
     1441 tatctttcat caacttttct agtaatcatt gggaaaaatt tcaagcagta gatcgcacaa
     1501 atacctacga cgatataaat gatcttcgca aaaatagttg ttgaaccacc agcaatcata
     1561 gctactaaat caagttcgaa caagcctacc aacagccagt tcaaccctcc cacaatcaat
     1621 aaagttaatg caatagcatc taaagttttc attagaattc tcctttctta aatgataaaa
     1681 caattatata tcaaaaagag aaacattgct tatataacgc tcaggaattt tagcaatcta
     1741 tgcaaaaaga tgaaaatttt ttttgctctt ccattagtac acatttttta gctgaagctg
     1801 taaatttcat gtcttaattt tttttagtga gcttttatac tggatttgta agaaaaaaga
     1861 aggagaggat cacatatgtc ggattggttg gatattgcag gaaaagtagt aattgtaact
     1921 ggaggttcct caggaattgg aagaagtatc gtcgaaaatt tgttgaagca gaatgcacaa
     1981 gtagcgaatt ttgatgtaac tgaatgccgt atacagcatg aaaacctact atctttaaaa
     2041 gtcgatgtgt cttctaaaac ggacatagaa gaagggatat ataaagtaat gaaacatttc
     2101 aagacgatcg atggtctagt caataatgca ggaataaata ttccttctct tctaatagat
     2161 agaaatcatc caaaaagcaa gtatgaatta tcagaacaag tttttgataa gatgatcgct
     2221 gtcaatcaaa aaagtgtata tttgatgagc caagcagttg gaagaatctt ggtgcaaaag
     2281 ggatcaggag tgatcgtgaa tctttcttct gaatcaggat tggaaggatc agaaggacag
     2341 agctgttatg ctgctacaaa agcagcgatg aacagtttta ctcgttcttg ggcaaaagag
     2401 ttaggaaaga ggaatgttcg tgtggtaggt gttgcaccag ggatactgga agaaaccggt
     2461 ttgagaacac aagaatatga agaagctctt tcttatacga gaggtataag cgtcgaacag
     2521 ctgagaaacg gttacagtcg aacaagtacg atccctctag gaaggagtgg taagctgcaa
     2581 gaagtggcgg atctagtctg ctattatctt tcagaaagat caagttatat cactggagtc
     2641 acgactaata tttcaggagg taaaacaaga ggataaagga taggggggac gcacgtgacg
     2701 ctagtaaaca gatggtacca attattgaaa ttactagtca ctcacaagaa agtttcaatc
     2761 caggaagtgc aaaaagaaat caaaaccagt aatcaaacca taaaaaaaga tattggggag
     2821 ttgaatgaag aaattgccgg tattgcaaaa atcatccaaa aaaacaaaca ttttgaactt
     2881 gaaatttatg attttgattt ttttgatgag atcatgcaag gaaaattgaa aacagccagt
     2941 gatttcaatt cggcatccaa aagaatcgct tatttactaa ataggctgat cgaaacagag
     3001 gatttcttac gtatggatga tttgtctgaa gaagtcggtg tcagcagagg aacagtggtc
     3061 aaggatctaa agacattaaa agagatgatc aatgattttg atgtaaagat tacaggcaca
     3121 ccaaataaag gactaagaat aataggagat gagctagaat tacgactact tttattttat
     3181 tttgtttctc cctacttttc agagacgaaa acagaacagt ttctagaaga aaaacaagca
     3241 atcaaacatt ttcagaagaa gacgaatgct agtaaacaag aaatcaccct tttaacgaaa
     3301 gttatcggta tttctttgaa tagaatctct agtaactatc tgttgaagaa gcccatcaat
     3361 cgttacagtg ggtgttttga atatatccaa gaattcaatg aactgatcaa tcagttggaa
     3421 gaaatctatg aactaacttt gagccaatat gaaaaagatt ttatcagttt tcctttgaac
     3481 atcatcaaca caagtataac gatgataagg aaagctaact ctgatttgcg cttatatttc
     3541 gaccccatga tgatgcgtat tcgacgatta gatctcattg atctggatga gtccttctta
     3601 tatcaagaga tgaaacagca cctctcttta ttgataaatc gggtaatttt tcgttatcga
     3661 ttgactgatt tattttatgg tgagatagaa aaaaagtatc ctttttctta ccaaatcgct
     3721 agcgaatgca tacaagcatt agaagctatc ttgcaacata agatctctaa agtagagatc
     3781 agctacctaa cgttgtattt ggagttagca tttcgatatc aaaaagagca tttttcctct
     3841 aaaaaagtag ccattgttca tacgacagga aaaggaacag cattaatgat ccaaagacaa
     3901 atcaaaagga tattggggag ggatatacaa ataacacatg tttcagaaga acaatatgaa
     3961 aaagtagatc taaacaaata tttcgctgtt tttacaacga ttccgttaaa aaatgtaaat
     4021 gaagagacac cggtgatcca tttgactaat ctttttgatg actcttggtt aagggcacaa
     4081 tggcaaaaag caaaaaaaaa gcgattagcc gggtttcatt ctactgtttt tttctatcaa
     4141 atatggaaag aaaaaaagac gtatcaagaa tatttaaaca aattagtcgc tcctttgatc
     4201 agaggctatt atgtggatga agaatttgaa aaaaggatct tgaaaagaga atatcagcaa
     4261 agcacatttg ttaacaaaga atttgctttt cctcacacaa ccaaccagct atctcaacac
     4321 attgttttat cagtagggct aataactgat caaggtttag cagaaacagg aggaaatgga
     4381 gttatttttt tatttgccat acctgaagaa atgacggctg aagttgagaa tgaattattt
     4441 gagctatacg atatcctatt tgaactcata aaaatgggaa tagatgagtt tattgaatta
     4501 gcaccgacac aagaatcatt tttaaaacta atcaaaagta aaggagagag acttcatgaa
     4561 tgatatgtat ctattcatag ttttcgcagc ttcagcttat gtgctgcaag cagttatcag
     4621 ttggaagcaa ctaaagcatt tcaatcaaac atatagcgaa ctgcgaagaa aaggaaaagt
     4681 agcgattggt aaacgacctg ggaaaatccg ttcaggcacg attgttatgc tagctataga
     4741 cgatcatgat caagttttag aagtcagaaa aatgcaaggg gttacatctt tggcaaaatt
     4801 tcattcatta cctgtctatg cgggtgaaga tatccattac atcgatcgtt accatccctc
     4861 agttcgagcg gaagatcgtt tgacacaagc agctttagaa aatgccagag aagtatactt
     4921 acgaatagag gcaggaaatg aaaaaagtga acaaccttca ttgcctttta ttgctatgct
     4981 caataatttt cggatatgga aaagtacgat caaagaaaaa aggagtgaca aacgatggat
     5041 atcataacaa aatttgctga gggatttatg aggctgttcc aaacaggtgc agaaacattc
     5101 ataagctgga tgacaggaat cgtaccagtt gtcttgatgc tgatggttgc aatgaatacg
     5161 ctgattgctt ttttaggtga agaccgagta acgaaagtag ccaaattatc agcgaaaaat
     5221 ccgcttactc gttaccttgt gttaccgttc ttatctgctt ttatgttggg aaatcctatg
     5281 tcatttacaa tggctcgttt ccttccagaa tactacaaac caagctatta tgctgctcaa
     5341 gctcaatttt gtcatacgag caatggtgta ttcccccata ttaatcctgg agaattgttt
     5401 gtatggatgg ggattgctca agggatcgaa accttagggc taaattcgat ggagctggct
     5461 atccgatact tgcttgtggg actgctgatg aactttattg gtggttggat aactgatttt
     5521 actactggat ttgtatgcag acagcaagga atcgttctta gcaaaaaagt agagctttca
     5581 gttgattaga gaaaggagag ttttaaatgt atggtgatat taagatcgta agaggaaacg
     5641 gcgggtatgg aggtcctttg attattaggc cgacaaaaga taaacacaag ttcatctatg
     5701 tcacaggtgg aggtgagagg ccaatgatcg tagataaaat agaagaactg acaggtatgg
     5761 agtctatcaa tgggttcaaa acatcaatac ctgatagtga aatcgcgctt gcaatcgttg
     5821 actgtggagg gactcttaga tgtggaattt atcctaaaaa agggatacca accatcaata
     5881 tcgtttccac aggaaaaagt ggcccattag cacaatatat cactgaagat atttatgttt
     5941 cagcagtagg gttagaacaa atacagccac taatggaaac tgatgataac aaagaaacag
     6001 taaatactgc atctccagat aaaaagtaca aatatgatac ttctaaaaag ataaccgagc
     6061 aaaaagcaga gcagcaaaac tggatagcaa aaattggaat gggcgcggga aaagtagttg
     6121 ctacattcaa tcaatcagca agagaagcag tagatacgat gatccggaca gtgatccctt
     6181 ttatggcttt cgtttccctt ttgattggtg tgatacaagg ttctgggatc ggtaatgtat
     6241 ttgctaacat tatggcacct ttagcaggaa atgcagttgg attaacaatc attggcttta
     6301 tttgttcact gccattttta tctcctttgc ttggtccagg ggcagttatt agccaagtga
     6361 ttggaacatt gatcggtgta gagattggga aaggaaatat tcctccccaa ttagcattac
     6421 cggcactatt tgctatcaat actcaaaatg catgtgattt tattccagtc ggtctaggtt
     6481 tggaagaagc aaaagctgag acagtagaag taggtgtacc atcagttctt tattcaagat
     6541 ttcttaacgg catcccacga gttatcattg cttgggtagc aagttttgga ttatatagct
     6601 g
//
LOCUS       DACCQI010000133         6436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCQI010000133 DACCQI010000000
VERSION     DACCQI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.139"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(153..1193)
                     /locus_tag="IVO76_002462"
     CDS             complement(153..1193)
                     /locus_tag="IVO76_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7430398.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1542..1868)
                     /locus_tag="IVO76_002463"
     CDS             complement(1542..1868)
                     /locus_tag="IVO76_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430399.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(1855..2643)
                     /locus_tag="IVO76_002464"
     CDS             complement(1855..2643)
                     /locus_tag="IVO76_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7430400.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3011..3301)
                     /locus_tag="IVO76_002465"
     CDS             complement(3011..3301)
                     /locus_tag="IVO76_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7430401.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3437..3616)
                     /locus_tag="IVO76_002466"
     CDS             complement(3437..3616)
                     /locus_tag="IVO76_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430402.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(3613..3963)
                     /locus_tag="IVO76_002467"
     CDS             complement(3613..3963)
                     /locus_tag="IVO76_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430403.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(3956..5281)
                     /locus_tag="IVO76_002468"
     CDS             complement(3956..5281)
                     /locus_tag="IVO76_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7430404.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            complement(5652..6236)
                     /locus_tag="IVO76_002469"
     CDS             complement(5652..6236)
                     /locus_tag="IVO76_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7430405.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1942 a         1171 c          928 g         2395 t
ORIGIN      
        1 ttaaaaccta cgattgtgat tgttttcata aacttttaat ctaaaaactc cggaatacta
       61 actatttatt tgtaatattt gtaataaaat taaaatcata aaaaagttct aatttaaaat
      121 tgatcatttt tatagtaatt gctttttatc aactactcta accaattttc cgtactaact
      181 gttggtaatt cgttaaatgt ttctctttgt tcgtttgcaa gctttgccgc aatagctttt
      241 tcgacaaacc aattttttac ataaacgaaa attagattct gcaaattgtt tactttttca
      301 gttttttgtt tttgataagc tttcagtaaa gtcatatata atcctttgtc agcatcaatt
      361 ccataagatt ccagttcttc ataaacgaca atagtttgct ctaattcttc agcttttttc
      421 tttgcattat gaatagtttg gacggtttgt tgagcttcac tgtaacttga agaaaatgtt
      481 tttatcaagt ttaatacatt ttctggtaca aatgtattta tactgtcctc ttgcatgatg
      541 tcgacgaagt tatctagcaa aacttgattt tgtaactggt cagtttcagt gtctattatg
      601 tgtctattag tgtctataga attagaatag aatagattgt ctgcgatttt cgcaattacg
      661 gttgagtcct tgatatgact ggattttgcc gtatttgcga ttttcgcaat tacggtagaa
      721 tcattgattt ttcgcggttt tgccgtattt gcgattttcg caattacggt ggaatcagta
      781 ttgtcttcct cttccatgat tttaaagacg tcttgattcg taacttctgg cttgcctaag
      841 tacaataaat taggcataga ttcaatctta ccatcacgtt tatttactct tcctctcttc
      901 tgtttcagaa gacctgcttt ttctaattct tttttgatct tagatacttt accttcgcgg
      961 cattgtaaca atttcattaa ttcttcaact gtgaatatga agaatatatt atcatcagaa
     1021 tctacccagt tatttttgac tgaataatca aatcgatctt taagtagcat ataagcaatt
     1081 tttgcatcat ttgaaagatc catgtatttt tcgttagtaa aaaatacctt aggcatttga
     1141 taatattttt cacgataaat ttcttttgat ttgtaataat taaaatcatt catagccaat
     1201 cctctttctg taggatagcg ttttgttcaa aactatgtta caatgaatta aacaaatata
     1261 tctatccact atatattttt ctactgctga taagtcgcca aactttgtta aagcagtagt
     1321 ctttttttat ctttttttag tttaccttaa aaaataaatt aaagcaaacc gctctatttc
     1381 agggtttaag ctcatggtaa ctatatcacg atattacaat aacattatat cacgatatcg
     1441 taatattaca atatgaagat aagcgactta tcagctttga tactactgat ttttataaaa
     1501 aaaatatcac gatattatga tatcgtgata ttgaatgtaa attaaatttt attttgtttc
     1561 tttgctagtt tatatccttc aatcaagtat tcttcaggta tggattttaa tagcgcatct
     1621 tctataacgt ctggtttact tttgtctgat tcagcaatag tcgtccaata tttgagtgct
     1681 gacattagaa ttggatttat acgaatgcta tctgctgttc ttacactact tccttgatct
     1741 tttatgactt tacttatatt tttcttggtt aattcgattt cttctacttc ttttggtttg
     1801 tagttctttt tatttttatt tcctagtagt ctacttactg cagcttgact gcgattatct
     1861 tctgccatca aaatcaccaa cttcttttaa atattcttct gtaaatgctt tatagtcatc
     1921 ttcagctgtt gagggtttat taaaattgat tagatctgat ccagcagctt gcgcttcgtc
     1981 aacggttatt gttctacgaa tataagtttt gaaaattttt gcaccaatga ctctagaaat
     2041 ttcggttgct gcagtcgtca tttctttaga aatgttataa cgcttattat acttagtaaa
     2101 taggactcct cgtacttcaa gcttgtcgtt cgtatattct tttaccgttt caatagtttc
     2161 acttaacttt attagtcctt ttaatgagaa atacgcagct tcaactggaa tgataatttc
     2221 atcagaaaca gtaaatgcgt tgactgtaag cattcctaaa cttggtggac aatcaattat
     2281 aatataatca tagttgtcct tgattggttg aagtactctt ttaagcctgt gttcacttcc
     2341 tacttgagta aattccctct ctgcagaaga aagggctatg tcggctggca aaacatcaat
     2401 tcctttatag tccagaatag catctgtaat gtcagcctcc ccttttaata cttcataaat
     2461 tgtaggttgg tttgttccat ctatatcgaa acctacatta tctgtagcat ttccctgagg
     2521 atcaatgtcc actaaaagta catttttacc gtaaacaaat ttcaaacatg ctgctaaatt
     2581 aacgctagtc gtagttttag aaactccacc tttttgattg gctactgaca atattcttgc
     2641 cattaaaata tcccccattc atcttatatt ataatataat tatatcataa tattgtgata
     2701 ttgcaatatc gtatatgcat tatattgtaa tattgtgtga ttattttgtt ttaaagatat
     2761 atatcggttc ggatataaag attattcata tgttattcga aagtaaaata atcacttttt
     2821 attactttaa aattttttat taatcataag aaatatgttt tgcgagcagc aactagaata
     2881 atttaaacat tattaaactg tctatattgt tactaataat tcccttctta tagtgcattt
     2941 tcttagaaaa aaacaatatt acgatatcat gatattaaga tatcgtaata ttatacaatt
     3001 gtgattgata ttatatatgt ttttttattt ttttatcatg tttaatcttg aaggaatcat
     3061 attctttttc taaagacagg agcttttcat aaacatcaga catgtttaaa ctagaggcca
     3121 ggcttaaatc ataaacgaat gaagcaggta atgattttat tctacgttgt cgggttatcc
     3181 agcttgcaac cgttccttgt gggtaggcat ataactcaca aaattgttct acagtgattc
     3241 ctaaattctt gataatgtaa acattgatcg ggtgcgagta aacaaatgta ttgatagaca
     3301 tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa taatagttta acacacgcat
     3361 tattctagta tctactccta tttgtaaaaa caaaaaagga ttcatagaga taactctata
     3421 gaatccgtaa aataccttat atatataacg ggaaaatata aaacctaaaa ttatgataac
     3481 ctgctgcagc ttctacatag aaagagaagc gctcaacacc aacctcattt atcaaacgtt
     3541 ggccataacc ccaaatctct tctgatagct gatcaaaggt tatatacttt agctcatatt
     3601 gctggataat gttcattcta atcgactcca tttattatcc tggtaaaatt cgatatgacg
     3661 tatttcgtcg tagtgaattt tgtcttttcc aacgtagatc cccagctcat cactacctaa
     3721 gatcttgcca acaatatctt ctggatagta tccttcagcg tttcgttctt ctttttgaat
     3781 agatactgat ctatttttta tgactgcagc gttcaatact tccgcaatct cttcagtcgt
     3841 catttgttct tttgctaaat tttgactggt tcgttgttca ttgtcggcat caatttcttc
     3901 agtatgatca gataaataaa accctgccca ctttaaacgt ccacgatcat tgtaatcaat
     3961 catttttaat tcccccattt ccgcccgcat gaccaccaac aagtgtgctt ctagtaatgc
     4021 ttctagcgct ttctaacatt gaagaagcat gtacaatcga cgtaaaacca tatttttgcc
     4081 gaatcttgtc aataatttta tctaaatttt cttcgttaat ttgcttttgt ggatcactaa
     4141 ataaatccag ctgaagcgaa tcggtgtaca caagcttgct ataggttatt ccaacgtgcc
     4201 ttacttcttg tccttcataa tacttatcaa acaagaaaag acaataattc tgcaattcct
     4261 ttgtattatc tgtgataggt attttcatct ggtgtgaaaa tcctggtctc gtttctaaga
     4321 tcgacgtacc aatgtttaaa tgaacacatc ctgttttaca attatgctgc ctgatacgaa
     4381 tggctacttg ttccgccatt tcttttacca ccagctcgat ttcatttctt ctagtataat
     4441 ctcgtggtaa cacttgtgaa ttaccataag atttttcctt tgtcggttct ggcgggattg
     4501 ctatatctgt tcgatcgatt ccgttagcat ggaagtacag ctgtaatcca atcacaccta
     4561 atctattctt aatcgtataa ggattccagt tagcaaggtc tctaatgctc aaaattccca
     4621 tttggttgag cctttttttc attcgagaac caattcccca aaagtccgtc atttcgggaa
     4681 tattccatac tttctcagga acgttttcgt acgtccattc cgcaataaat ccttcattat
     4741 gctttgcttc attgtctaag gctaatttag ccaaaagggg attatcgccg acgcctaccg
     4801 tagcaatcaa acctaactct tctttaatac gttcttggat catttgagcg agcttcttcc
     4861 ttcgctgact tcgtgtttct tcagtcgtaa aaagattcag tgattttgtc acttttaaaa
     4921 tcgattcatc gatcgagtaa atcagtagat cttcatcagc cacatatctt ctgaaaatat
     4981 tatttacctg catatttcgc ttgatatata ggttcatacg tggtggaaca acgtgtagtg
     5041 ttttaggaaa tggttgtggt aagtcacgtg gtctactcac atttgtaata ccataccgct
     5101 tttttgcttc aggagaagaa gctaatatca atcctgaacc agtattatca gatcgactca
     5161 taacaacaag ttctgttgtt aatggatcta aatttctttc tatacattcg acacttgcgt
     5221 aaaagctttt catatcgatt aaaaagtaat catttactgg ctcttttgaa taatccatca
     5281 taaataacac tccattcttc cacattatac aaacatatgt tcgatgttgt aaagaagaaa
     5341 tagctaaata cataaaaaaa gaagcatagg tatgctatca ccctgcttct tcttatgatt
     5401 tgtatgatca atttattcta tcactttact aattaatttg aaaatctaat aaaaactctc
     5461 actctaaaag agtgagagac tgcaaattag agtacggact tttttggagt tccgttcttt
     5521 aatattaaca aatgaataaa atccttacaa catttgataa taagttatca gtagacactt
     5581 acaaaaaatt ctcatactat tgtcactttt ctagataaat ggcaaaaatt ttcagcatta
     5641 aatgacaaaa actaatgaga atttgtcttt ccatttttct cctccatttt tgccaaataa
     5701 cgataaatgg tggttcttga gacgttccac cgttccgcaa tagtcttaat aggtgtaccg
     5761 ctatccacta aagtcttcag taattccaaa tcttgttcag acaatttttc aggacgacca
     5821 ccaaaccgtc ctcttgcacg agcagctgct cgtcctgctg cggatcgttc caggatcaaa
     5881 ttacgctcaa attctgcaaa tgccgcaaac aaatgaaaca tgagctgtcc tgtagaactt
     5941 gatttgtcca tcgtaatatt ttcttgaata ctatgaaaac tcacaccacg ctcattcagt
     6001 cgattgacga ttgaaatcaa atcttccata tttctaccta aacgatctaa gcgccaaacg
     6061 acaagtgtat cgccagagcg aacaaaatca atggccattt ctaatcctgg tcgattactt
     6121 tttgccccac tcatatgatc agtaaaaatt ttttcacaac caaatttatt taggctgtct
     6181 tcttgtaaat ccaaattttg taaaccagta gatacccggg cgtatcctat tttcaaaaaa
     6241 atcactcctt cattaatttt attgtaacat aactttatcc atatagttta actgaacata
     6301 gatattgtta tgggtttatg aacatgaaat gaattgtttt tttgaaaaat aaagcaaaaa
     6361 aaggaagtgt ccagaaatgg gtgattttat gaacactttc aattagaaaa aaagaatgtc
     6421 gactagacac tctttt
//
LOCUS       DACCQI010000134         6423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCQI010000134 DACCQI010000000
VERSION     DACCQI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.140"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..374)
                     /locus_tag="IVO76_002470"
     CDS             complement(<1..374)
                     /locus_tag="IVO76_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430406.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVEMKKAKTVSSEWLDY"
     gene            complement(390..641)
                     /locus_tag="IVO76_002471"
     CDS             complement(390..641)
                     /locus_tag="IVO76_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430407.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            complement(641..847)
                     /locus_tag="IVO76_002472"
     CDS             complement(641..847)
                     /locus_tag="IVO76_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7430408.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            complement(1296..1556)
                     /locus_tag="IVO76_002473"
     CDS             complement(1296..1556)
                     /locus_tag="IVO76_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430409.1"
                     /translation="MEHYTGLLDKIRVIKTQPLLVRFTLRTNHEPINCVVANIEIADK
                     LLILEEGKYTISVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            1631..1834
                     /locus_tag="IVO76_002474"
     CDS             1631..1834
                     /locus_tag="IVO76_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP7430410.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(1975..2460)
                     /gene="ssb"
                     /locus_tag="IVO76_002475"
     CDS             complement(1975..2460)
                     /gene="ssb"
                     /locus_tag="IVO76_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7430411.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFVNCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKATTESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(2463..3362)
                     /locus_tag="IVO76_002476"
     CDS             complement(2463..3362)
                     /locus_tag="IVO76_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7430412.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFADTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(3375..3968)
                     /locus_tag="IVO76_002477"
     CDS             complement(3375..3968)
                     /locus_tag="IVO76_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP7430413.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(4057..4221)
                     /locus_tag="IVO76_002478"
     CDS             complement(4057..4221)
                     /locus_tag="IVO76_002478"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430414.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMETKTAAI
                     KEPAADSKRN"
     gene            complement(4240..4662)
                     /locus_tag="IVO76_002479"
     CDS             complement(4240..4662)
                     /locus_tag="IVO76_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430415.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDEIISTHAEVKNYSEAVRFLCMNYD
                     PEMEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEARERVE
                     GKIKSNQTKKYSPQLKKPVNESVYARVDPFDPTKILKR"
     gene            complement(4664..4960)
                     /locus_tag="IVO76_002480"
     CDS             complement(4664..4960)
                     /locus_tag="IVO76_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430416.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLIENN
                     FDRPLMEYKKEKFDQLLEKFSEIQSYNTKILEEYVSQNNRILEILIEQNRGSEL"
     gene            complement(5252..5464)
                     /locus_tag="IVO76_002481"
     CDS             complement(5252..5464)
                     /locus_tag="IVO76_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430417.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
     gene            complement(5485..6336)
                     /locus_tag="IVO76_002482"
     CDS             complement(5485..6336)
                     /locus_tag="IVO76_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430418.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIIHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
BASE COUNT         1758 a         1267 c         1004 g         2394 t
ORIGIN      
        1 aagtagtcca accattcact tgaaacagtc ttggcttttt tcatttcgac aaatttccca
       61 tacgttagtt ctaaagcttg gtattcagaa ggtttgatag tgtttggctt gacgcctgct
      121 tccaagcata gtaaggcttg gaaaaaccat tgtccatttt gctttttcag ttcttcaagc
      181 gttcgatagt aggtcgcaat gagattcatt tcgacacccg attcaatcat ttcttccaca
      241 gctaccgaca atctttcctt ataatgtttt tttggaacac gtgtctcatt gtataagaat
      301 gtcatatcct ccattttttg tgccatttta tccaaaggta atttgacaaa ttcgtcatag
      361 taattttgag ccatattttt ttctcctttt tagttctcta atttggttaa ccaatattcg
      421 ttttcataag tttccatgtt ttcttgataa tgcagttcat atcctgctat cttcactagg
      481 tcttcaaata gcatagtcgg tgcatcctct actaaagggg tttctatcgt ccagtaccgc
      541 ggaataagta aagcttctgc tactttttca gaaatgactt ctttgtccac caaaatttga
      601 acgacttctt ttacagtcaa taatttgtgt tgtgttttca tcagcgtttc tttcctttct
      661 catcaatcaa aatataatcg ccataagaca aggtatattt tttcccgcat gttggacatt
      721 gatacaaacg atagcttttg atacatttcg ctctttctcc acaatcgcat tcgacagtat
      781 gtggtgcatg atagtttggg aataattcgc tgaagggaac accccaataa tcaaatttct
      841 ttcgcatgat tgattctgcc tcctcttagg aaaggtttta cttcctttcg ttagttcttt
      901 ctttattgat ttgtttgcca gaatgaatca acgaaaagcg acaaggacaa gggcgtgcag
      961 cgtgccattt acccttgtcc ttgtcgcttt ttggtgataa aaggcaactt gttgccggtc
     1021 tggcaaatca ataaagagat agcgaacagg aacgaacctt ttggtgagag gaagcttttc
     1081 ctattttgtc ttacaaagca agacccctta tcttagtgaa cgtgcaacgt tcacttcctt
     1141 tcttctcgaa cggcggtggc ggttggagtg agagaaaggt ttttcaattg agaaggcgct
     1201 tgtgccttcc taaacaggcc tttccctttc ttcctgtttc tatgtaaaac aaaaagagct
     1261 gcagtcctac attaggattg cagctcttct tttatttata tgcccattga tcgcgtaaaa
     1321 tgatcagggt ttctaatttg cattgattca atgactagtt gatttcgttt attgaagtga
     1381 cctgtcactg aaatagtata cttgccttct tccaaaatca gcaatttatc cgctatttct
     1441 atattggcca ccacacaatt aatcggttca tgatttgtac gtaatgtaaa tcgcacaagc
     1501 aatggctgtg tcttgattac ccgaatttta tccaataaac cagtataatg ttccatcgcg
     1561 atcacctcgc taaaattata gcaaaaacag ctaaaattga gaatttttat tcgattttag
     1621 atatgctata atgatcaaga cagtaaagtt tgtgacggtg gctatccgat tgaaagtgag
     1681 gtggtgcgta tgtgcgtact tccaaaatct tacagaaagg agaagccttt tgcctgttga
     1741 aacggcgcta ggattaatga ttagtttcgg ttctttgatc gcaacaatca tttttggaat
     1801 cttagcagtt gtaaacaaca atcaaaaaaa ataaccgtca gctttagccg gtcgacgatt
     1861 atttttcaaa aataatatta agctggtcac cgtcttaaac ggtgctgtta ggggggttgt
     1921 tagcgcaact cctcttttcg tatccattat aacaacttta ttgatctttt acaatcagaa
     1981 tggtaaatca tcatcactaa tatcgattga ttgtccgcca aatggatttt gtgaagcgtt
     2041 caatccattg ttgttaggcg tagctacatc ccgttgttca aaggtggtag ggcttggact
     2101 gctggacgat tgatcagcgt gcgcacggct ctctgtcgtt gctttacttt ctaatagttg
     2161 aaagttatca gctacaactt ctgtcacata cacacgttgt ccttgttggt tatcgtaggt
     2221 tcgggtttga atccgtccga caacgccaat caagctgcct tttttagcta atgtcgccat
     2281 cgtttctgct ggtttgcgcc aaatcacaca gttgacaaaa tcggcttctc gattgccgtt
     2341 ttgattcgtg aaattacgat taacggctaa ggtaaaagtg gccactgctg tgccacttgc
     2401 ggtataacgt aaatctggat ctttggttaa ccgtcctact aaagttacat tattaatcat
     2461 ctttatctct ccatttcttg attcatttgt tgtgatcttt taaactgcct ttttctttct
     2521 tgattttcaa ttttgatcac acttttcatt aggtgatcaa tttcgttgac tgtatctgca
     2581 aatgaaatgg aattcgcaaa cgtgttaact gagcgatatt tttcccactg cttttccaaa
     2641 tagtcgtttc ccctgatctt atccatttct ctttcataat aaaaggataa aggctttgta
     2701 tctccacgtt ttgtaaatgt atttcgaaca cttttgtata actctttcgt atcaatattt
     2761 tctagtttcg gaatggtaaa taagtcgtag taatcttttg ctctcgaatt atctgtgcca
     2821 aagctgaacg tagcacttag cttttctgca ataatttgtt ctgttggata agctggaatc
     2881 tccacttttt tatcttcaaa cattaattgg tgatagtgtg tatctacttc aggataaata
     2941 gaatctcctg ttgtcacgtc cattttaaac ggtacacgta ctttctctaa attagccacc
     3001 aatttaattt gaaatccagg ataaaaatcg gcttctcttg tttccttcat tccttgaata
     3061 gagaaaacaa tgccttcttt tgtgggtttt ctagttaaat cattaaaaat gctcttcaat
     3121 ttttcttcag ttaatttgct atttctaaat gtcgtatcga tatcaattgt tgtccttcta
     3181 tcgattccat attttgatcc taataaaaag ccgcctttca aaatgaagtc atttttgtac
     3241 tttgattcag aaatttgttc aattagacga tccatcataa aatgccgttg cactaactgt
     3301 gcatttaatc ctgtactctt tgctaaattt cttgtttttg ctgcaaattg tgtcggtgtc
     3361 atctttttcc tccatcaata taagctttgc atgtggtatt tcatttcttt ttctacatgt
     3421 agttttgtca tgtaatctct taatttactt ggatctctca atgggtcttc catgtactct
     3481 tttaatgctt ctaatttcat ttcatattca atttcatatc tcggattcca aatatcacaa
     3541 agtgtacgct ctttatcata catagtaata ggatttcctt gaaaggaagt tccctctact
     3601 tttcctagtt cataaacttc tttcttcacg aaatgaaatt gtaactgctg ctcttttgta
     3661 attgaacgat caacgttata gccatgggga aaagttaagt gaatgaattt cggcatattc
     3721 gtagaaagct ggtaaatatc taaagtcgtt tcatgagaga agattcccct ggaaaatcgt
     3781 tgctgaagcc aaaataaatc atctattcca tgaccatcct ctacataata accacgatca
     3841 actttgatca attttccttg attcaccaat ctttgtacag tggttgctgc gattcctaat
     3901 tttctggctt ccttcataga taaatttcca tcgcgaatag ccaacaattc tttcagtttc
     3961 tcttgcattt acttcccccc cttttgttac aaatacacta cattaaatat atcatgatag
     4021 tgtatttgta acaaggttaa tgtctatcga tctaattcaa tttctttttg aatccgctgc
     4081 tggttcttta attgctgcag tcttcgtttc catttcttcc agcgcttttt ctcttgtcat
     4141 ataatcaatg tatgttgaaa aagatttttt attgggcaag gtaaattgac aagtgagaat
     4201 aactgcaggc gatttattca tcatgttccc cttctacttt tatcttttta gaatcttcgt
     4261 tggatcaaat ggatctactc tggcataaac agattcattc acaggtttct tcaactgagg
     4321 ggaatatttt ttggtttgat tagatttaat ttttccttcc acacgttctc ttgcttcttg
     4381 gtaaaccgtt gaatttgtac caattagtat tcctgtatct ttcaaatgaa gatctgcaaa
     4441 ctctgaaacc atttcggtta aaatagaaat ttctttactc attccattca actttgcttg
     4501 attggaattt acttttttct ccatttctgg atcataattc atacatagaa accgaacggc
     4561 ttcactatag tttttaactt ctgcatgagt agaaataatc tcatccaaca catcgatatg
     4621 tgtttggtga aagtaaatgt gtttgtctat aacctttgcc atatcaaagt tcacttcctc
     4681 tattttgctc gatcaagatt tctaaaatac gattgttttg gctaacatat tcttctaata
     4741 tctttgtgtt atagctttga atttctgaaa acttttctaa taactgatca aacttttctt
     4801 ttttatattc catcaaagga cgatcaaaat tattttctat tagtaatttt aaataatcgt
     4861 tacgactcca tttattaccc gtacggttac ttagttcgtc acatcttcga tcaatttctc
     4921 ttacgatttt ctcatccaca tttcttattc ttacttccat attaattcct ccaatttaaa
     4981 aaggatagcg aaaaggagcc tatccttttt gctatcctac taccctcctt ttactatcct
     5041 tcactatcct ttcactatcc tatttttaaa tattttgttc ccacatttat ttttgagaaa
     5101 cgtaataggc acagcctatg ggaacacttt ttttaaaaag tgttcccacg tttccttatg
     5161 ctctttacaa ataaaggctt ttagagacgt tctggaattt tagattcctt gaattgttcc
     5221 cattcatcaa tcaattggct ataagtagcc attaattttt ttcttgctct tgttgctttc
     5281 gaatcaacaa aaaatcagtt tgattcgtta attctaaata ttctgttcgg tattcaatgt
     5341 tcatcgtacc cgttttacta catagtaact gattatgacg ttctttcact ttgaataact
     5401 gctccttctt taaggttttt aactccatgc caactataag cacaatcctg tatagaggaa
     5461 tcattttcaa acgattctcc ataattattt atctttcatt ggaagttcta aatcactact
     5521 gttccaaaaa atgtatggtt tgttttgacg aatattactt ccatctaacc acaattttcc
     5581 gatgccatat cgtaggcttg cttcgacttc ataaaaattg tagatctctt ttcttcgatc
     5641 tattgctttc atttcctggc cattttttaa aatagatctg atgcccttaa cagtagattc
     5701 atggatactc gtgtgaatga tatccgtatt gatcaattcg tcatatcttc caaaatcaac
     5761 attgtactca taaccaagaa gtgtcggaat caggtcaatt tcaatttctt tttcaattga
     5821 acgtttaata agtttcgtgg atacataccc gacaaaaaag agaataatac ccacttcaaa
     5881 caaatacatg tgtccattta caaagtgaat aaacgtcaat aaaaaatcct tcactccttt
     5941 tgttgttctt ccagattgtt caatttgttt tattaaagtc gctagaattg catttacaac
     6001 aattaaatcg aataggcttt tgaaaagttt tcctgctgtt aatttttgcc tggtaaattg
     6061 catgactaat tgagtaaaaa tagacaatag catgacaaaa atcaacgtca ttagcactgt
     6121 aaaaatgtct gatgctgcaa tcctattggg atcatttatc aagtaataag ccgttccaaa
     6181 tactccaatc gagtaaacgg ttaaatcaag aataagtcgt gccccgtaac tacaaagttt
     6241 tcctaatggc cataattgca atacccaaaa tttctttcgt ttgctatttc ttttcagagg
     6301 aagccaggtg tgttcaatcc agtcgtccga tttcatttcg atttcttccc taaattcttt
     6361 cctttaacgt tttcctttgc tactttagca gcgataggtt gctcaatgaa ccaatttttt
     6421 aag
//
LOCUS       DACCQI010000135         6385 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCQI010000135 DACCQI010000000
VERSION     DACCQI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.142"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..90
                     /locus_tag="IVO76_002483"
     CDS             <1..90
                     /locus_tag="IVO76_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7430419.1"
                     /translation="NCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            352..1167
                     /locus_tag="IVO76_002484"
     CDS             352..1167
                     /locus_tag="IVO76_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7430420.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            1177..1839
                     /locus_tag="IVO76_002485"
     CDS             1177..1839
                     /locus_tag="IVO76_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7430421.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            2044..3708
                     /gene="alsS"
                     /locus_tag="IVO76_002486"
     CDS             2044..3708
                     /gene="alsS"
                     /locus_tag="IVO76_002486"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7430422.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            3721..4431
                     /gene="budA"
                     /locus_tag="IVO76_002487"
     CDS             3721..4431
                     /gene="budA"
                     /locus_tag="IVO76_002487"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7430423.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            4561..5058
                     /locus_tag="IVO76_002488"
     CDS             4561..5058
                     /locus_tag="IVO76_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7430424.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            5243..5503
                     /locus_tag="IVO76_002489"
     CDS             5243..5503
                     /locus_tag="IVO76_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430425.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(5865..6104)
                     /locus_tag="IVO76_002490"
     CDS             complement(5865..6104)
                     /locus_tag="IVO76_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430426.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT         2076 a          974 c         1313 g         2022 t
ORIGIN      
        1 aactgcatgc tagtctggaa aaagaatcgg attttatcac ctgtagtcaa tgaattgatt
       61 caccgtttca aagaagcttt tgaacagtaa taagtatctc taatcgaaga aatttcaata
      121 caaaagcgag acgtatgaca gtcttgcttt tgtatttttt catttttaaa tcaaaaaaaa
      181 taaagagatt tacataactt gttaaataag cgcttacaaa cataagaaga acggaaatga
      241 atcagctata ataaccgttg ttctggattg taatcattac aaagaaatga agattataac
      301 tttggaggtg ttcgaaatga aagaaaaaaa gcaaaaattc gttcaaaaaa catgcccttt
      361 attcaatggt tgggtatgat tctgctgatt gtattagctt tattgacgat cattcctttt
      421 ttatggatgg tttctgcttc attcaaagca aataatgaag tgttcacgat tccgattcaa
      481 tggatcccta aaacttggca tttagagaat tatctggtta tttggtctcg tattccttta
      541 gtgacatttt ttaagaatac agctttttta agcgttgtga tcacgttgat tcagttgttt
      601 acttctagtt ttgctgctta cgggttttct aaactaaatt ttaaagggcg cgatatttta
      661 tttttagcat atatcggcac gattgctgtc ccttggcagt cttatatgat cccgcagttc
      721 attatgatga gaaaagtggg tttaacatat acgttgtggt cgttggtgct attacaagcg
      781 ttcaatgctt ttggtgtgtt cttgttgaag caatactaca gtagtatccc agattcttta
      841 tgtgagagtg cgcggatcga tggcttatca gagtggggaa tctatcgcaa gatcatttta
      901 cctcttacaa aaccggcgat tgccagtttg acgatcatca cattcgttaa tacatggaac
      961 gattatatgg ggccattcat ttatttgtct tctacggaaa ataaaacaat ccagttaggg
     1021 ttgaaaatgt ttattgggct atttgatgca gagtacgcat taatcatggc tgcctcagtt
     1081 gtttctgttc ttcctgttgc ttttatattt ttagcgatgc aaaaatattt tgtagaaggg
     1141 atcgcttcat caggaatgaa ggaataggag gtgattatga aagagcagcg ttttgacaaa
     1201 aatgtttata tggaattctt ttattggttg tatcaatcat tggcaagtag cctttgtttt
     1261 tggtgtgtca atatcttgta tatagcagct gcgttccttt tgtcgataga tggaaggaat
     1321 gtcttttcct ttattttttc tttacttttt attggtccag gttgcatcgt gatgataggt
     1381 atgacagaag aattagcaaa agaaaaaagt ttttcaccag tacgtttgtt cataaagacg
     1441 tttcgccaat tttggctaaa gggatttttt tattggctat ttgcttggat agttagtgtg
     1501 ataatgattt ttgattgttt cttttttata cgtttttctt atggaaaatg gttgattccg
     1561 ttgtttgttt tacttgcttg tttatcagtt tcctttagca tcaactactg gtattttcag
     1621 gtgagaaatc cagcgtcgaa accaaatcaa gttctacgga tagcgttcta ctatacgttg
     1681 aaaaaatggt atgtgagttt actagatttt ttactattga caagtttgtt tctcttcttt
     1741 ttcgtaaagc cccaatggtg tattctacta ggtccttcta tcgtatttgg tttgatttac
     1801 ttcaacaatc gtaaattgat gagaactatg gatctgtaga ctttataaat tgaaactttc
     1861 aattaagaat gacagttttt atgtaaaaac tgtcattctt tttcattttt tgaaacatat
     1921 ggctaaacaa atgaaataaa acgtgctata cttttgaatg aaacaggttt agaaactgta
     1981 tcaattattt gtgattattt ctaactaata attaaaaata aagcataaaa aggacgatgt
     2041 tcaatggacg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt gattaatcat
     2101 catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt taatgaatta
     2161 gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc atttatggca
     2221 caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag tggtcctggt
     2281 gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc agttttagca
     2341 attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag catggataat
     2401 gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc agaaacaatt
     2461 tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg cgcgagcttt
     2521 atcagtatcc cgcaagatgt agtggatgca cctgtccaag ggaatgtcat caagccattg
     2581 agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc tgaacgcatt
     2641 agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga aaaagaaacg
     2701 ttagccatca ggcaattagt tggaaagaca gcattgcctg ttgtagagac attccaagca
     2761 gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg tttatttaga
     2821 aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgctat tggctatgat
     2881 ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat cattgtgatc
     2941 gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt gatcggtgat
     3001 atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt gtcagaagat
     3061 gcaaaagaat atttagcatc tcttcaagca gaattaacgg agcgggacat tgttcaatca
     3121 aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca gtcaaaagta
     3181 acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat ggcacgccac
     3241 tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac gcttggtgtt
     3301 gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacaaat cgtatcagtt
     3361 tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt acgtaaaaaa
     3421 ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga gttccaagaa
     3481 aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt tgtgaaatat
     3541 gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt agaaaaagct
     3601 ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat tgattaccgt
     3661 gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg aagtgtcgac
     3721 atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc tggcttgatg
     3781 gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt gggaacattg
     3841 catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg aagatcagat
     3901 ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat tacagatttt
     3961 acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt gaaaaaagac
     4021 atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac tggattattc
     4081 aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag actagcaaaa
     4141 atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt agtcgggttc
     4201 tttacgcctg aattattcca aggagttgct gcagcgggat ttcacttgca ttttatcgat
     4261 gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg aaaagttgag
     4321 attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac ttttgttcac
     4381 gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata gaaaaagaca
     4441 gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga ggaaacctag
     4501 cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc ggtttttatc
     4561 atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca gcaacaagga
     4621 ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc attgttgaat
     4681 gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa tgaattgatt
     4741 ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa aggagtttgt
     4801 ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac ccatgcaaat
     4861 tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt aaaagaaggt
     4921 aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga tgaaatcgat
     4981 caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat aaatgctttt
     5041 tcatttatta ataactaaaa tcgtctattg aaacaaaaaa gaagagaaat caagaataaa
     5101 aagagccgga aagacagtgg gcatttctgt ttttcggttt ttttattttt ttataaaatc
     5161 acaagtgtta ttgattttca taatcatatt ttaatggtat tatcatttta aataatggaa
     5221 aaacattaag gatggatgaa atatgaataa cgaaactatc attttaggaa aaaaatatat
     5281 ttgccaacct attggcttga aacaaaaagt ggttggagaa gtaatcagta aattagaaaa
     5341 ttgtgttgtg atctgcgtag acgaatatca tgacgtcgat cgcaatgaaa tattagaaaa
     5401 atgcggaaaa gtagtggcaa aatatactca cgtttatgag ttggaaacaa cagaatatct
     5461 atataactat agcaacaaat atgaacatac tttagtttca taaagattta aataaagtgc
     5521 cttcaaaagc gagaagacca agcttttgaa ggcactttat ttagttggca gtgattgttc
     5581 atgatttttg atttattcaa aaggttcgcc tagagaatct gtctctgtaa cgaggcctgg
     5641 tggagaaact cgataaaaac ctatactgcc agaactgtcc tattttcgga tagacaaact
     5701 agtagtttta aaaagatcat ccgatccaat catttcaaaa aatgcaaaac tgatatcagg
     5761 atcatttagc tcaggcttgt tatcgtaaag catttgtaaa ggttgcattt agttaggagt
     5821 aaacgcgaaa ctatcatcat tatttgctga agaagatgaa gaagctaatt cagagtttgt
     5881 ttgaaataaa ggagacgtgc tgacagtttc tttaccctgt tttttgcaga ggtagaaact
     5941 tttatatcgg aagtttctac cggagaattt ttctcatttg tagaaattgt tgaatcgata
     6001 ctattcgaaa tttgcgtatc tgaaggtatg ttctttgatg cagaagtccc acaagcagta
     6061 agtaacaata aatttgataa actgataaac agaattcttt tcattatgta cacactcctt
     6121 atagtatcgt ttagcataca cctatgctca gtattaggca aaatcaatgc aaaactatca
     6181 agtcttttat agtatctatt agtcttagta atacatatat ggagagggtg atatcccagt
     6241 tcaaaccaga tacattggtt accatctgta ttaacgatta tgcctctaaa cgcttttttt
     6301 atgaaaaaat ttataagtga aaacgtaggt atgatattat ctagaggtaa tttttttggg
     6361 tatttaaata gttgctagga ggata
//
LOCUS       DACCQI010000136         6297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCQI010000136 DACCQI010000000
VERSION     DACCQI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.143"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(12..305)
                     /locus_tag="IVO76_002491"
     CDS             complement(12..305)
                     /locus_tag="IVO76_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7430427.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(377..2104)
                     /locus_tag="IVO76_002492"
     CDS             complement(377..2104)
                     /locus_tag="IVO76_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7430428.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEEAKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            complement(2318..3244)
                     /gene="trxB"
                     /locus_tag="IVO76_002493"
     CDS             complement(2318..3244)
                     /gene="trxB"
                     /locus_tag="IVO76_002493"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7430429.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(3389..4822)
                     /gene="ascB"
                     /locus_tag="IVO76_002494"
     CDS             complement(3389..4822)
                     /gene="ascB"
                     /locus_tag="IVO76_002494"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7430430.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQVKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(4822..6225)
                     /locus_tag="IVO76_002495"
     CDS             complement(4822..6225)
                     /locus_tag="IVO76_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7430431.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
BASE COUNT         1653 a         1468 c         1086 g         2090 t
ORIGIN      
        1 tgcacctatc gttattgatg ttccacatgt ttgagtgtct ggttcaaaat atccagtaca
       61 tgagaatcat caagactata caaaatcgct tttccctcgc gatgagactt gacgacatga
      121 ttttctctta ataaacgtag ttgatgcgat acagcagact gctccatttc cactgcttgg
      181 gtgattgctg tcacattttg ttccccgtct ttcagatata gcagaatttt aagacgagtg
      241 ggatcactca acattttgaa taattgactg actttctcta cttttgattt ttgcgtgtct
      301 ggcaatccat tcacctccaa cttgaaaaaa gactgacttc taaagtttac cactttttcc
      361 atcagtctca agttttttat tcttctgtca tctttttgat aatcgcttca taagaggcta
      421 ctttttcggc tgcgtcttct gtagagcttc cttttacacc aatataaaac ttgattttcg
      481 gctcagttcc tgaagggcgg atagcaatcc agccattttc ttccaatgta tatttcaata
      541 cgtcggatgg cggtgttgtt agtttttcta cgtttccttc acggtctgtt ctcgtcaatt
      601 ctttaaagtc ttctgtttga ataacttttg ctccggcaaa ttctgtcggt gcttctttac
      661 ggaatttttc catcaaagca gagattttcg cagaaccttc ttgtccgctc attgtcacag
      721 aaatcgtttt ttctgcaaaa tagccatagg tttcaaaaat cgtttgcaag ccgtcataca
      781 acgttttgcc ttgtttttta tagtaagctg ctacttctgc taataaaagc aatgcttgga
      841 tcgcgtcttt atcgcgaaca aaaggtttaa ctaaataacc ataactttct tcgaatccaa
      901 acataaaagt atgtgaatgg tcttcttcga attgttggat tttttcagca atgaatttaa
      961 accctgtcaa cacattgaac atggtagcac cgtagtgttc cgcaattgct gtaggcaatt
     1021 cgctggaaac gattgatttc aataccgctg cgttttctgg taaagtgcct cttgattgat
     1081 gtgcttccaa aatatattga gcaaacaatg ccccgatttg gttcccagtc aatacttcat
     1141 aagaaccatc tggcaaacga accgctgcgc ctaaacggtc tgcatcagga tctgttgcaa
     1201 tcagcaagtc agctccttct ttttctccaa gtcgtatagc atattcaaaa gctgaatgtt
     1261 cttctggatt tggcgatttg accgtagtga aatctggatc tgccacagct tgttcgggaa
     1321 ctagtacgaa ttgttcgaat ccagcttgtt tcaacgcacg ttcgcccaac attttccctg
     1381 ttccatgaag tggcgtgtac acaagtttca aatctttccc cattgtttct accaattcac
     1441 gatcaatcgt cactgctttg atttcttcta gataagcagc gtccacttct tctccgatga
     1501 tcgtgatcaa cccgctatgt ttagcttctt cgtcagataa tacttcgact tttagcggat
     1561 tgctgacttc acggacatat ttcgtcaatg catctgcgtc tgcaggaggc atttgtccgc
     1621 catcttctcc gtagacttta tatccattgt aagccgctgg gttgtgagat gcagttacca
     1681 tgattcccgc aaacgtcttc aaatgacgaa ctgcaaaaga aagctctggt gttggacgca
     1741 gactttcgaa tacataagac gggatattgt gatgtgccag tgttttggct gcttccattg
     1801 caaattctgg tgactgatgg cgtgagtcat aagcaatagc gacacctcgg cgttttgctt
     1861 cttcaccttg tgtatccatg aaacgtgcta gtccttctgt tgcttgtctt accgtataga
     1921 tgttcattcg attgatccca ggtcctaaga ttccccgcat gcctgcagta ccgaattcca
     1981 acggtgcata aaaagcatct tcaagttttt caggatcttc acccagatca tttaattgtt
     2041 tttttaagtt ttcttctaaa ttttcttcat gtacccattg ttcatacact tgttcccaag
     2101 acatgaaaat aacacctctt tctgaaatat ggttcttaat aagtatagca ttcacttatt
     2161 agatatgaaa gtatgaggac caaaaaacgc ttaaatcaag aaaggattct tttatcaaaa
     2221 ggtgttggac gtgttcgcat aaatcagatt cagtaaattt cacgcgaaaa aaaagagtaa
     2281 aagaacaatt gaacccgttc ttctactctt tttaaactta tgcagttgtt tcttctaaag
     2341 attccaagta tttatacact tgttgtccag caataccgcc gtcaccaaca gctgtagtga
     2401 tttgtcgcaa atctttttca cgaacatctc cgatggcaaa aacgcctggc atattcgttt
     2461 tcatttcgtt gtctgttgga atccagccag cttcgttcgt caaacctgct tctctaaaag
     2521 gtgcagttaa tggatccagt ccaacataga tgaatactcc gtctgctgct aaatcaaatt
     2581 cattgccggt tttgacatta cgtgctttca cacctgtcac taccatttcg tttccaacga
     2641 tttcgtcaac gactgtatcc cacataaagg aaattttttc atttgcaaat gcacggtctt
     2701 ggatgatctt ttgtgcacgt aattcatctc tacggtggac gatcactact tcagaagcaa
     2761 attgtgtcaa atagatggct tcttctactg ctgagtcccc acccccgatg actactagac
     2821 gtttgttacg gaaaaacgca ccatcacaga ctgcacagta agaaacgcct cgtccagcaa
     2881 attcttcctc gccaggtacg cctaatttac ggtggataca gcctgttgcg ataatgactg
     2941 tttttccttc gtaactttta tcttcacaga tgacttcttt atggctgccg tgatccttga
     3001 tatcttgaac gattccataa gcattttctg tcccaaatct tgagacactt tcatacatct
     3061 tataagctag atcggggccc atgatcgaat cgaatccagg ataattttcg atttctgccg
     3121 tattgttcat ttggccgcca ggggcacctt gttcgatcat caatacggat aagtttgaac
     3181 gtgaagcata taaagcagca gtcatcccag caggacctgc gccaataacg attacatcat
     3241 acatatgtag attctccttt acttaatcaa gtaagcatac tttacagcca atgcgaaaaa
     3301 aagtctattg ttctgcttgc gtttctcgaa tagctttatt atactcgaaa aatccgctga
     3361 aaaacaatag acttttataa atcattgatc aatcgttcga aagattttct ccatttgaat
     3421 caattacttt tttataccag ttgaatgatt ttttctttga acgggctaat gtgccatttc
     3481 cttggttatc acgatcgaca taaatgaatc cgtatctttt cttcatctca ccggtaccag
     3541 ctgatactaa atcaatacat ccccaagtcg tatagcctaa tagatcgact ccgtctagtt
     3601 ctactgcatc cttcatcgcc tggatatgcg ctgccaggta atcgattcgg taatcatctt
     3661 ccacataacc attctcatct ggcgtatcga ctgcacctaa gccgttttct acaataaata
     3721 atggtttttg ataacgatca taaatatcat tcatcgttat tctcaagcct aatggatcaa
     3781 tttgccaccc ccattcactt gcttgcagat atggattttt gacagaagca aagatatttc
     3841 ctgcagtctg ttccaagagt tcgttgtcgg tagtcgaaac tcgggaagaa taataagaaa
     3901 aagagataaa atcaacagta tgttctttca gcaatgcggc atcccctggt tccatttcga
     3961 tctgaatgcc atttcgttcc atttctttca atgcataagc tgggtattct cctcgtgact
     4021 gaacatcaat gaagaaataa ttttctcgat ctgattttct agaagcccaa acatcttcag
     4081 gattgcaagt gtatggataa tatgctcctg ctgccagcat acagcctact ttgttctccg
     4141 gatctacttc atgagcaatc ttcgttgcga tggcacttgc aaccaactca tgatgtgccg
     4201 cttgatattt gacttgttct tcgttttctc cttcttcaaa gtacagtcct gctcccatga
     4261 atggtgcatg aaggatcata ttgatctcat tgaatgtcag ccagtatttg acaagtcctt
     4321 tatatcgatt gaagatgact cgacacagat tttcataaaa accaacgagt tctctggaac
     4381 gccacgcacc gtattctttg atcaaatgca ttggacaatc aaaatgcgta atcgtcacaa
     4441 gtggctcgat tccgtgcttt tggcattctt taaagacatc ctcataaaat ttcaagccag
     4501 cttcattcgg ctctttttca tcccccattg gaaaaatacg actccaggcg atggacattc
     4561 gataagtctt gaaccccatt tctgcaaaca aagcaatatc ttctttataa cgatgataca
     4621 tatcgattgc aatttttgct ggataaaaat gatcctcatc aaattcaaac atttttttct
     4681 ttccggtgat aacaggaaaa cggtcttttc cgattggcac aacatctacg tttgctagtc
     4741 cgcgtccacc ttcatcataa ccgccctcac attgattggc tgctgtagca ccgccccata
     4801 aaaaatcttt tctgaatccc attatacttc ctccattata tgtgtattac ctgataattc
     4861 tcttgccagt tcatcttgaa cttcgattcc ttcttctttt aatcggctga ttgtttcctt
     4921 gaattgcggt aaatactgct ggtgtgcaat aaacaactca tccatgatcc gttttgctgt
     4981 ctttcctgat cgtataagcg gattgatcgt aaacgcctgt aaagctgtcc cataatcacc
     5041 agtcacagct gcttcgatcg tcagtaattc catattcttc attacttgca accagcctct
     5101 ttcagcagca ggaagagaac caaacgctac atttctcgct ccttgtgcgc caacataggc
     5161 agtgacttct actacacaat ctgccggcag gtcaggaaca gctccatcat tctttgtaga
     5221 taccacgatc tgtgtttctt tatttccata gattgaagca atcgtttcac aggcagcgtc
     5281 tgaataataa gcgccacctc gtttttgcaa ttgttcaggt ttatggtcta attctggatc
     5341 tttgtataat tcaaatagct ctgcttctgt ttctttcact tgctgcgctc ttgtaccaat
     5401 cgtttgatat tcttctaaag catgttccag catttcttct tcacggtagt aatagcggtg
     5461 gtagccacaa gggatcatat tcatttgttt taattgttct ttgaaaaacg ggatatcaaa
     5521 gatatttttc ggcaatccat tgtcttcatc gtataaacga tcaatcaatt ctgccgtgcg
     5581 gtcatttcct ttattatctg ctactttgtg ccaatggaaa tgatttagac cggcaaactt
     5641 gtagatcagc tcttcttttt tcttgccaag cattgctggt tctgtcatca tcgccattac
     5701 tggcacattg cataatccaa tcactttatc ccatttgcca tatctgataa ctgcttctgt
     5761 caccatccca cttgggttcg cgaaattgat cagccaagca tttggacata aacgcttcat
     5821 gtcttcaacg attgccaaga ccactggaac ggtacgaaaa gctttgaata tcccgccagc
     5881 accatttgtt tcttgcccaa tcatcccata ataactaggg atccgctcgt ctttgattcg
     5941 tgcatctaat tgaccgacac gaaactgagt cgtaacaaaa tccgcatctt tcaatgcttc
     6001 ttcccgatcc agagttgcat agattttgac ttcatacgga gaagcatccc acatacgctg
     6061 agacatctga ctaacgatct caagcttttc ttttccttct tctacatcta cgagccagat
     6121 ttctcgtatc ggtaattcct catatctttt gataaatcct tccatcaact caggggtgta
     6181 actgctgcct ccaccaatcg tagcgatttt gattcctttt gccatgttct cactccttta
     6241 cttttttaca ctttcattat cttttttgta aacaaaacaa acaatagaaa tccgttt
//
LOCUS       DACCQI010000137         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCQI010000137 DACCQI010000000
VERSION     DACCQI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.144"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IVO76_002496"
     CDS             725..811
                     /locus_tag="IVO76_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7430432.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IVO76_002497"
     CDS             complement(932..1621)
                     /locus_tag="IVO76_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430433.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="IVO76_002498"
     CDS             complement(1866..2780)
                     /locus_tag="IVO76_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7430434.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IVO76_002499"
     CDS             complement(2762..3142)
                     /locus_tag="IVO76_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7430435.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IVO76_002500"
     CDS             complement(3592..3879)
                     /locus_tag="IVO76_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7430436.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IVO76_002501"
     CDS             complement(3899..4123)
                     /locus_tag="IVO76_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP7430437.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IVO76_002502"
     CDS             complement(4763..5296)
                     /locus_tag="IVO76_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7430438.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IVO76_002503"
     CDS             complement(5317..6159)
                     /locus_tag="IVO76_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7430439.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACCQI010000138         6169 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCQI010000138 DACCQI010000000
VERSION     DACCQI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.145"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(150..1829)
                     /locus_tag="IVO76_002504"
     CDS             complement(150..1829)
                     /locus_tag="IVO76_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7430440.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(1831..2043)
                     /locus_tag="IVO76_002505"
     CDS             complement(1831..2043)
                     /locus_tag="IVO76_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7430441.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            2436..4166
                     /gene="efrA"
                     /locus_tag="IVO76_002506"
     CDS             2436..4166
                     /gene="efrA"
                     /locus_tag="IVO76_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7430442.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            4163..5923
                     /gene="efrB"
                     /locus_tag="IVO76_002507"
     CDS             4163..5923
                     /gene="efrB"
                     /locus_tag="IVO76_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7430443.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         1848 a         1107 c         1299 g         1915 t
ORIGIN      
        1 tttttttgta tgaaaaagta aataaattta agaggctatg aaagagccac cttgtatcaa
       61 ggaataagaa caaacaaaaa tggcttctca tgtttttcat ttgttttctc ttatattcag
      121 gacaaggtgc tgatttcata acctcttatt tattcttcat ctggtgtcat taccattggc
      181 aaaatcattg gatgtctttc tgtacgttcg aataagaaag gctgaagcgc gccagtcatc
      241 gcttctttca atttatattc tgaacagtca ggtccattca aacattctct taaagcgtgg
      301 aataagattc gctgagcttc attgatcatt tctcctgatt ctcgcatgta gacaaatcca
      361 cgagagagaa tatctggacc agccaagatt tcttttttct tgatatccac tgtcgcgaca
      421 gctaatacaa gaccttcttc tgaaagcagg tgacggtcac gtaaaacgac attaccaata
      481 tcaccgatcc cgctaccatc gatatacaca tctccagcca caaaatgccc agcaggacgc
      541 gcagaatcgc tagttaacgc taaaacatcg ccattttcca tgatgaagca gttttctgca
      601 ggaacaccta catcttgtgc tagagaagca tgtactttca acatgcggaa ttctccgtgg
      661 acaggaataa aatattttgg cttcatcaaa cgaagcatta atttttgttc ttcttgtcca
      721 ccgtgaccgg aagtatggat gttattgatt tttccgtgaa tcacttctgc accagcttca
      781 gacagcaaat tgatcaaatg attgacactt gttgtattac caggaatagg tgaacttgaa
      841 aagatcactg tatcgtctgg ctggatttgg atttgacggt gagtaccatt cgcgatgcgg
      901 ctcaacgcgg ccatcgcttc tccttgagaa cctgtacata ggatcatcgt ttcattggca
      961 ggcaagcgat tgatttcgtg tgcatctaca aatgtgccgt ctggaacttt gatatatcct
     1021 aaacgttgac cgttgacgat ggcattttcc atgcttcgtc cgaaaacagc gattttacga
     1081 ccagttgcca cagcggcatc tgctgcttgc tgcaaacgga agatgttaga agcaaagctg
     1141 gcaaaaatga tacgtccttc gatttttgaa aagatcttca aaatagaatg cccaatcgtt
     1201 ttttctgatt tagtaaatgt tggaatctca gcatttgtac tatcagataa aaggcacaag
     1261 actccttctt ccccgatacg agccatttta tgcaagtttg ctggttcgcc aacaggagtg
     1321 aaatcgaatt tgaagtctcc agtcgctacg atatttccag aaggtgtttt caccacgatc
     1381 cctaatgcat ctgggatact atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa
     1441 cggatgatcg tatcttcatt gatctcatgg agctctgcat ctcgaagcaa gccatgctca
     1501 tctaatttgt ttgtgatcaa cgctaatgcg agaggaccag catagatagg aatatttgct
     1561 tgtttcagca agtagggtat tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag
     1621 gcttttactt tgtgtaagtt ttgtatgatg tagctgtagt cgggaatgac atagtcaatt
     1681 ccaagtaaat cgtcttccgg gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg
     1741 aattgtaccc catacgtatt tttcccgatt tcacctaatc caccaatagc aaagacgcct
     1801 gtctcgttat ttttgatgga aagtttcata ttaaaactcc actaatgaga aatcagcatt
     1861 ttctttttcg tactctagat gacttccttc tagaagttga acgaattcaa tattgtatgg
     1921 ggtattttct tccacacgtt ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga
     1981 ttttgtttct tcccgtttag ggttgcgtac ttttgtttct tgataataaa ctttatagat
     2041 cattgttttg tctcctttat ctgcgtaaag cagtgattta gctagttgat ttttttagac
     2101 aacacaaaac gaggggacaa gccacctggt ttttcatatg ctttgactag aatactggat
     2161 tcgagtcaaa gagacaagcc tcgtttttgc ttatctgttc ggatattttt tcaaccgtac
     2221 cttctgtata aaaaatatac aacaattaat tgttattcta gcataaaaca gcaaataagt
     2281 atagaaatca actagattga aagaaaaaat caatgtttat catcccgcta taatcataat
     2341 gactttttta tgagagaagt gttctattaa taggctcgtt gtgctatcat ttttagtaat
     2401 gaaaatgtaa caaaagatta aaaggagatg agaaagtgaa actaatgtgg cattatacca
     2461 tgcgttataa gaaattcttg tttttcaatt tcatatgcgt atttggtttt attttgattg
     2521 aattaggact tcctacgatt ttagcacgta tgatcgatgt aggaatccgc aatgatgatt
     2581 ttgattatgt gaagcaacaa gggatattta tgatcgttat tactgtaatc ggtattattt
     2641 tgaatatctt gttaggttat tttggttcga ggattacgac taatatcgta gcagatattc
     2701 gagatgattt gttcgaaaag atccaaagtt attcgcatca ggaatacgaa aatctaggcg
     2761 tatcttcatt gatcactcgt acaacaaacg atgcttatca gatcatgttg ttcctgcaaa
     2821 atattttacg tatcggtttt atggcaccaa tgatgttcat tgtcagtttg tacatggtca
     2881 tgcgtacaag tgtgacactc agtttgtatg tagtaggtgc attaccttta ctattattgg
     2941 cagtcgtagc gattgctaag gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag
     3001 ataaaatcaa cagtatcttg cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg
     3061 tcaatgaaaa gtttgaagaa aaacgttttg ctgtagtcaa tgagaattat gcttctagtt
     3121 caaaaagctt attccgtttg atggctgttg cacaaccggg tttttatttc ttatttaata
     3181 tcgtaatggt attgatcatc tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg
     3241 taggagattt gattgcgttt atcgagtata tattccatgc cttgttctca ttcatgttat
     3301 ttgctagtgt gttcatgatg tatccaagag cagctgtttc agctggacgt atccaagaag
     3361 ccctagatgc caaaccttcg attgtagaag atccaaatgg tatatctgat acaaaaacta
     3421 aagggtatat cgaatttcaa aatgtaacat ttgcttatcc aggccattcg caagagccag
     3481 tgatacgaaa tgtcagtttt acagcctctc caggagagac cgttgctttt attggaagta
     3541 ccggtagcgg aaaatctacg ttgatccaat tgatccctcg cttttatgat gtgagtgaag
     3601 gaaagatctt gatcgatgga gtagacgtga gagattatcg gctaagcaag ttgcgagata
     3661 agattgggtt tattccgcaa aaagcgctgc tatttactgg aacgattgca gataatttgc
     3721 gatacgggaa agaagatgct acgcaagaag aaatggaacg tgcagctgag attgctcaag
     3781 cttcagaatt catttctcga aaaccagatg gatttaatga acatttatca gaaggtggtt
     3841 ccaatttttc aggtggacaa aaacaacgat tggcaatagc cagagccatc atacgccgtc
     3901 cggaaatcta tatttttgat gacagctttt ctgcattaga ttatcaaacg gatgcgaagt
     3961 tacgtgctcg tctgaaaaaa gaaacgacag aatctactgt attgatcgta gcacaacgtg
     4021 tgggaacgat catgcatgca gataagatca tcgtattaaa tgaaggagaa gtcgtcggta
     4081 tggggacgca tcgggagttg ttggaaaaat gcccgattta ttatgatatt gccgcttctc
     4141 aattatcaaa ggaggaatta gcatgaaaaa aaccttttct tccttaaaac gtttagctcg
     4201 atatatcaga ccgtatcgtg agacttttct tttagtgatc ctatttacag ttctgacagt
     4261 ggctttcaac gtggctttgc catatgttac aggacttcct actacagaaa tcagccgtaa
     4321 tattgccaat ggcgaatcaa tcaatttttc ttatatattc aaatgtctca tttggatcac
     4381 agttgttgga atagggtatt gtatttcgca attactttct ggtgttttga tgacaaatgt
     4441 cgttcaaagt gccatgcatg atttgagaag agacatagaa gaaaaaatca atcgtcttcc
     4501 ggtttcatat ttcgataaaa accagcaagg aaatattttg tctcgtgtga cgaatgatgt
     4561 agatgcagtc agcggtgctt tacaacaagc ttttattggt atcgtaaatg cgatattggg
     4621 aattacgttg gctgcagcaa tgatgtttta tatccagccg atgatggcgc tgatctctat
     4681 gatcatgatt ccaacatcga tctggatctc aaaacgagtg atcaatgctt cgcagaaata
     4741 ttttcaaagc atgcagaatt ctcttggtga gctaaacggc tacgtccaag aaaatatgac
     4801 aggttttagt gtattgaaag tatatggacg cgaaaaagaa acattggaag gattcaaaaa
     4861 agttaatcac agcttgaaat actttgggtt tcgtgcagca ttcatttctg gcttgatgat
     4921 gcctctagtt caattaactg cctatgctac ttatattggt atggctgttc taggaagctt
     4981 ttatgttatt actggggcga tcgttgtcgg tcaactacaa gcattcatcc aatatatttg
     5041 gcaaatcagt cagccaatgg ggaacgtgac acaattgtct tcagttttgc aaagtgcatc
     5101 agcggcaacg aaacgtgtat tcgagatttt agatgagcca gaagaaaaag aaaacaaagt
     5161 agatgcttca ctgccagaaa cgattttagg agatgtgaca ttcgatcatg tcgattttgc
     5221 atatgatccg aagaaaccat taatccaaga tttgaatttt gaagtgaaag ctggtcaaac
     5281 cgtagctgtg gtcggtccga ccggtgccgg caagacgaca ttgatcaatc tattgatgcg
     5341 tttttatgac gtagacaaag gagcaataag gattgatgga atcgacacca aatcaatgag
     5401 tcgcagtgat gtgcgttcgc tatttggtat ggtcttgcaa gatgcatggc tttatcaagg
     5461 aacgattgca gataatatcc gcttcggaaa gcttgatgcg acagattatg aagtagttga
     5521 tgctgcgaaa acagcaaacg tagatcattt tatccgaacg atgccagatg gatatgatat
     5581 ggccatcaat tctgagggag acaatgtgtc tttaggacaa aaacagttgc tgacgattgc
     5641 ccgagcagtg gtctccgatc ctaaaatctt gattttagat gaagcaacaa gttcagtaga
     5701 cacccgttta gaagcgttga ttcaaaaagc aatggatcgc gtgatggagg gacggacaag
     5761 tttcgtcatc gctcaccgtt tatcgacaat ccgtgatgca gatctgattc tagtgatgga
     5821 tcaaggtcag atcattgaaa aaggaacgca tgagagttta ttggcacaag gtggatttta
     5881 tgaaaaactg tataacagcc aatttgctga ggaagcagaa taagtcatat aagaataaaa
     5941 cagtttaaaa cttatttttt gttctctatt cttgataatg gtaaagtaaa gttatcaaga
     6001 aaagtgagga cacggagatg gaacagtttt taaatgaaaa aattactgaa gccaaaagag
     6061 aagttgatcg tttaacgcac gtctgtcaga cgaagccttt tgattcagct gatttttttg
     6121 aaaatgagtt tcaaatcgaa cgcttggcag cacaaataga agtgtacga
//
LOCUS       DACCQI010000139         6112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCQI010000139 DACCQI010000000
VERSION     DACCQI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.146"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            58..510
                     /locus_tag="IVO76_002508"
     CDS             58..510
                     /locus_tag="IVO76_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430444.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="IVO76_002509"
     CDS             524..1213
                     /locus_tag="IVO76_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430445.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..3689
                     /locus_tag="IVO76_002510"
     CDS             1338..3689
                     /locus_tag="IVO76_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7430446.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3769..4164
                     /locus_tag="IVO76_002511"
     CDS             3769..4164
                     /locus_tag="IVO76_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430447.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4489..5106
                     /locus_tag="IVO76_002512"
     CDS             4489..5106
                     /locus_tag="IVO76_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7430448.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5147..5446
                     /locus_tag="IVO76_002513"
     CDS             5147..5446
                     /locus_tag="IVO76_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430449.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5480..5647
                     /locus_tag="IVO76_002514"
     CDS             5480..5647
                     /locus_tag="IVO76_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7430450.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5692..>6112)
                     /locus_tag="IVO76_002515"
     CDS             complement(5692..>6112)
                     /locus_tag="IVO76_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7430451.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2110 a          837 c         1226 g         1939 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     3661 ttcaagaaaa tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt
     3721 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
     3781 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
     3841 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
     3901 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
     3961 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
     4021 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
     4081 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
     4141 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
     4201 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
     4261 tttatttaat ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat
     4321 aatcatgcgg gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt
     4381 ttctttgttt atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa
     4441 tctgattgtt ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt
     4501 ttttatatcc gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag
     4561 gaattaggaa tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa
     4621 cgccctgaat atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa
     4681 tcattagata gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact
     4741 aaggaaaagc agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac
     4801 aagcaactag atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat
     4861 atggccgaag acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg
     4921 aaacaaaagg gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg
     4981 atctattctg actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc
     5041 atgaaacgaa atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt
     5101 tcttgataga tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa
     5161 tcaaagggtc gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt
     5221 ggttcactct ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt
     5281 ttgtggtcag tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac
     5341 cgcgcgggac ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta
     5401 tgcgttaatt agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt
     5461 tacaaggagg aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac
     5521 tagttttaat cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag
     5581 tgtttatcaa aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg
     5641 aacatagaaa cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt tctacaaatc
     5701 attcgctttc tttattttat aaatagttgg tcgagagatc ccgttttctt ttgcaatctc
     5761 tgcaactgga atgccttgct ttaacatgtc caccacggtt ttaaaaacta agcgtttttg
     5821 cggatctgtt gagttaatag aatactcgat tggtcgtccc ttatatcggc cggctttttg
     5881 agccaataat actccttgcc gctgacgttc acgaattttc tcacgctcgt tttgtgcaat
     5941 ataacttagc agtgataaaa acatatcgaa catagcggta tccaggagtt catttccagt
     6001 attaaaatct aaaaactgtg catctaatat ttttaacgct acttttttct gtttcataaa
     6061 agcaactgta ttttttatat cttcataatc tcttcctaaa cgatctagtg at
//
LOCUS       DACCQI010000140         6078 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCQI010000140 DACCQI010000000
VERSION     DACCQI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.147"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="IVO76_002516"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="IVO76_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7430452.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="IVO76_002517"
     CDS             complement(2204..4390)
                     /locus_tag="IVO76_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7430453.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="IVO76_002518"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="IVO76_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7430454.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="IVO76_002519"
     CDS             complement(4612..5052)
                     /locus_tag="IVO76_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7430455.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="IVO76_002520"
     CDS             complement(5126..5665)
                     /locus_tag="IVO76_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP7430456.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1576 a         1369 c         1068 g         2065 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagg
//
LOCUS       DACCQI010000141         5976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCQI010000141 DACCQI010000000
VERSION     DACCQI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.148"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(58..360)
                     /locus_tag="IVO76_002521"
     CDS             complement(58..360)
                     /locus_tag="IVO76_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430457.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(580..946)
                     /gene="ssrA"
                     /locus_tag="IVO76_002522"
     tmRNA           complement(580..946)
                     /gene="ssrA"
                     /locus_tag="IVO76_002522"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1117..1890)
                     /locus_tag="IVO76_002523"
     CDS             complement(1117..1890)
                     /locus_tag="IVO76_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7430458.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2034..2384
                     /locus_tag="IVO76_002524"
     CDS             2034..2384
                     /locus_tag="IVO76_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7430459.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2365..3081
                     /locus_tag="IVO76_002525"
     CDS             2365..3081
                     /locus_tag="IVO76_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7430460.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3081..4529
                     /locus_tag="IVO76_002526"
     CDS             3081..4529
                     /locus_tag="IVO76_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7430461.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4599..5108)
                     /locus_tag="IVO76_002527"
     CDS             complement(4599..5108)
                     /locus_tag="IVO76_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430462.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5098..5823)
                     /locus_tag="IVO76_002528"
     CDS             complement(5098..5823)
                     /locus_tag="IVO76_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430463.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2030 a         1042 c         1161 g         1743 t
ORIGIN      
        1 cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta gcctacttta
       61 atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac gccgatgatc
      121 gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat ctttcttcat
      181 tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga gattgattaa
      241 taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat aaaaatcttt
      301 gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt ccatcaacat
      361 aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta ggaatatcat
      421 tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct attttgaatg
      481 ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac ccttataaaa
      541 taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg gcgggagtcg
      601 aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac tcatttaagg
      661 attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga tgttctcttt
      721 ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc ttactcgagc
      781 acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa gcgtaagagt
      841 tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg taacctacga
      901 aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta gctaacaagg
      961 gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat tcaataaaaa
     1021 agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa aaataagcga
     1081 ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa aatacttcaa
     1141 tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa gttcttttaa
     1201 tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca aggcaaaatc
     1261 attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt ctttttgcag
     1321 acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg agtaaggacc
     1381 attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac ctgattgaga
     1441 taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa tttccggaac
     1501 atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg tacaccagat
     1561 tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat catacatttt
     1621 ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta ctcgaatgaa
     1681 agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag agtggatttc
     1741 tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta atgcgttcat
     1801 agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc catcaagatc
     1861 aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat tggaataagt
     1921 ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct gaacatattg
     1981 gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa agaatgattt
     2041 ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt ctgccctcaa
     2101 aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca gacgctcatt
     2161 ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta ctgatggcat
     2221 tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt gaaatgcttc
     2281 tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta cagaaatatg
     2341 acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa tcaatctgtt
     2401 tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt caagtatgat
     2461 ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat gattacacta
     2521 gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg caaagtttca
     2581 agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca ggtgatcggc
     2641 ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac gcttattgct
     2701 cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga agcagtagtt
     2761 tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa aacacgagaa
     2821 gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga tctattgttt
     2881 actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga attagtctgg
     2941 gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa attacgacaa
     3001 aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg ctacaaattc
     3061 gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct ttctgcggaa
     3121 tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca aaacaaacga
     3181 ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa aagacaacgc
     3241 aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa aatgcattgc
     3301 tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc gataaaaacg
     3361 aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca gctgatcaat
     3421 ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat attttaggac
     3481 aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa ttctatggag
     3541 cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca attacactga
     3601 acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc tatgcttttg
     3661 cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa gtgacgaatg
     3721 gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag ttagcagatg
     3781 atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa gaacaagaaa
     3841 atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct cttacgacaa
     3901 tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag cgagaaaacg
     3961 cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat gaaaacttgg
     4021 attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc aatggaacag
     4081 aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca ggagatacat
     4141 tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga tttgtccaaa
     4201 tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa atcacgatta
     4261 ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga attggaatgt
     4321 ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct tcaagaaaaa
     4381 atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt gtacgcttgc
     4441 ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc gctgttcgtt
     4501 tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat catgaatcga
     4561 tcttctatac cgcttgtttt tactataaaa tgattgattt agcccgcggg ctctaaacga
     4621 tcaatataag gactttgagc aatagtagca tcatgagtaa agagtaagat accttgattt
     4681 tcttcatgag ctaaataaga aagcaagtgg agtagctctt ggatctccaa tacactaagt
     4741 tcatcaaatg tatcttcaat aacaatattg tttcgcttgg aaagcaaggc atgaagtaat
     4801 tgcagcttta ctttttcaaa agacgtcaac tcagctgccg cacaattaag caaggaagga
     4861 gagatatcaa gtttttcttg ataggccaaa cgatcttgtt tgatttcttt ttccggtaca
     4921 tccaacagga catggtcttt aagattcaga taaggaaaca tcggccaatt tttttggatg
     4981 atcgcaaaat tatccagttt tgcaacaaga acctcttttt tcaagtgctc caatatttta
     5041 tgctgttttt cgagaggaca caaaataacg gtcaagtgtt cagcatgaat caaatattca
     5101 ggtttcatta cgtccaggcc tcctactttg caaatgatgg ataaaaaaag tgaaagggaa
     5161 tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa atcccttaag
     5221 tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct gttgatccat
     5281 cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca gttcagtagt
     5341 ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact ggaagcttac
     5401 gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct gccagaaaat
     5461 aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca attcctgctt
     5521 gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata gaaaaaataa
     5581 actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa gaagctctgt
     5641 agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta aagccgattg
     5701 caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg aaaataaaca
     5761 atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc ggattatttt
     5821 catacttttc acctctataa aaagtataga aaatgaatat gaataaagta tgaaattcct
     5881 ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag gttgtgaaga
     5941 agctgcccta ttctattacc gtagatgcaa gctttt
//
LOCUS       DACCQI010000142         5871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCQI010000142 DACCQI010000000
VERSION     DACCQI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.149"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(327..1445)
                     /locus_tag="IVO76_002529"
     CDS             complement(327..1445)
                     /locus_tag="IVO76_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430464.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1916..3223
                     /locus_tag="IVO76_002530"
     CDS             1916..3223
                     /locus_tag="IVO76_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7430465.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3342..4541
                     /locus_tag="IVO76_002531"
     CDS             3342..4541
                     /locus_tag="IVO76_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7430466.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4568..5836)
                     /locus_tag="IVO76_002532"
     CDS             complement(4568..5836)
                     /locus_tag="IVO76_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7430467.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
BASE COUNT         1804 a         1246 c         1049 g         1772 t
ORIGIN      
        1 ccgaaatttc acaaaaattt gagaaataat ttttgtcaat ctcttcttat ttcttgaagc
       61 tgaccacttc tgtcacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa
      121 taagcccacc aaacaaaaaa tatgaagagc tatttttgct tgctgttctt attacttgat
      181 gcagaacagc tttttcacaa cctctcatac acggtgttta atcagctgac cttcggtatt
      241 agctcaaaaa acggaaaaat atgtgaaact atttttccgt ttgtttctct aatactcagg
      301 tcaaaacact gatttcacaa cctcttttaa taacgaagaa tcgctgtcag actcttttca
      361 tctggcgcat ctttttgatc caaaataaac actttcccgc cattaatgat cacatcatct
      421 gctaatgcat tcaacacttg acgccgatca tattctgttt caggatcttc accgaaacca
      481 tcagctaagt ttgacgtagc gataaataaa tgtgacactt ttccttcttt cgcagctgga
      541 acgatatcaa ctaactgatc gatgaatttc ttatctaaga gtttattgta ttcttttgat
      601 tcttgagctg cgagttcact tgacagcttc ttcgcccctt cttcgatatc tctaaaggat
      661 agctgcgcag gagaagcaga aatcgcaacg tctgcacaat aatagggatt tttagcaatt
      721 tttttgaaca tcgtctgatt ttctggtaat gcaaataaat aaatcggcca ttgttcagga
      781 ttttcgaaag aatccttcaa gtacgtatca acagcttggt aatagttgac ccaatcgatc
      841 tctacttctt catcttttgg atttacgcca tggaaagtta ctccatctcg ggctccgcct
      901 cctccattag aagagaagtt caaaccgcca cctgtcagtt cagatcccaa tgcagtaacg
      961 acatcaaccg gtgccccttc tggtagagat acttctttta cttgtttgtt gctgacctca
     1021 tacaatttca tggagtcgcg gttcaatccc aataaataat aagaatagtt gaactgccca
     1081 tttttgataa tagccagtaa ataaggaaga tcacttacat agtattgatc gtctaccggg
     1141 atactcaaac gatggatata ggtctggtca gcagttataa tgaccgctac actcgtagtt
     1201 gcactacgcc aaaacgaagg atctcctaac aaattttcaa ttttttcttg gaacggggac
     1261 cagtcatgtt cggaatattt tttctcaaaa cgtttctttg cttctttcgc aaaatttttg
     1321 aatttgatct gatctttttc cacatcttga tgggcgacat gcgtattcag catgatcgtg
     1381 acgaatggtc ctttagcgcc ttctgaagtc aattgtgtca ataattcttt atcctttttt
     1441 gccataaacg tacccctcct aaaaaaatca ttcctttgtt tctatctaaa gtctaccaca
     1501 acactatttt tccatacaat aaaacgtatc taaaaaaata aaacggtttc tacggtttta
     1561 aatttagcta tttttttaag ttatcttcat aatttcctca attatttagc cccagtcttc
     1621 aactattctt catgaaatgc tgataaacta agaaaaagaa taaaagaggt ggatcttatg
     1681 catttgatta tgattttcag tttattaagt attatcagtt tatttgcttt cagcgcttcc
     1741 cgcttattag atgcggaaac acagcctatt cctgtaaaag tacgtaaaaa cgaccgctaa
     1801 acttccataa cgcgcgttct tatcctaaag ttttttcaga aacattgaaa ctctctcccc
     1861 tctctgataa aattttaatt tgtagcgaaa caaattaatt acaaggagat cggatatgtc
     1921 cataaaaaaa acacgtctag tcctattatt ggctagtctc attctttgcc ttgcttcttt
     1981 tctaccagct gttgctgtgc aagctgaaga tacgttcaaa gtaaatgcaa aagcagcttt
     2041 tgctgtagat gcgcaaagcg gtaagattct ttatgatcaa gatggagaaa aaaccatggg
     2101 gatcgcttca atcacaaaaa tcatcggtct ttacatcgtc cttgatcaag taaaagaagg
     2161 aaaactgtca tggggtgata aagtttcgat ttcagattat gcagaaaatt tgagtatcac
     2221 cccagattta tcgaatgttc ctttgcataa agaaaacacc tacactgtca aagaattatt
     2281 cgactctgca atcatccagt cagccaatgc ttcaatggtg gccttggcag aaaaaatctc
     2341 tggcagcgaa gctaaattca ctgaacgaat gaaagaacaa ttgaaagatt ggggaatcaa
     2401 agatgcaaca atcgttaatg catcaggatt gaacaactct tatcttggcg aaaaccgacc
     2461 agaaggcact ggagaaaacg acgaaaatca aatgtctgct caagatgtgg ccatcgttgc
     2521 ccgccatttg atcttggatt tcccagaaat actggatgta tcaagtacta cgacccaaat
     2581 gtttggcgaa aatacgcaat caccagtgga aatggtcaac tggaactgga tgctcccagg
     2641 attcgttaat tacaaggaag gcgtggacgg attaaaaact ggtacgactg agcttgcagg
     2701 tgcttgtttc gttggtacga tcactaaaaa cgatcaacgg atcatcactg tcgtattgaa
     2761 tgcggacggc catgcggaaa acccgagtgc ccgcttcaat gaaacagacc gtttgatgga
     2821 ctactgctat gacaattggt cagaaaaaga gctcggaaaa gctaacgcaa gtattcctag
     2881 tctaaaaacc attgatgtaa aagacggaaa agaatcaaca gttcctgttg tgcttaaaag
     2941 ccctgtaaaa gtatgggtac gaaacgatat ggatactggc aaattaacga tcacgccaac
     3001 acttgacaag aagcaagtca ctgacaatga gatccaagca cctatggata aaggtacaaa
     3061 aatcggtacc gctacgatca ccttggcaga cgataaatta ggctatttag aagacaacaa
     3121 tgcaccatca acagagatca ttacggataa aacagtggaa aaagcgaata tattcgtgat
     3181 tggctggcga cgcgtcgcag aatttttcgg caacttgttt taattgaaca ataagaaaaa
     3241 catctttcca tcggaaagat gtttttttgc tttttctagt ttaaataaca gcttttttca
     3301 tctattttcg ttatactgta taagaaaaag gagttgaatt tatgaatcta accagagatc
     3361 tgatcttttt agttctctat cttattgggg tattctctat cgtttattgg cgaaaacaga
     3421 agaaggatcc ttccttgttc ttttacggca gtacgtggac atttacggta atcttaactt
     3481 attttgtcgt gttggaaatt ttttattcga ccttttattt tttgattcca ttgttgttct
     3541 ttagtatctt tgctttttct tatttcacag aaaaacgaag gctactgaat ggtcttttat
     3601 tcaatgtttt actcatcagt ttcggcatct acttgtttgt cttgctgtat gaaacgcaaa
     3661 atatttttct tggcggactg atcgccttga tcacgattcc attgctactt gtccttttat
     3721 ttggtattta tggattgatt gtttttcttt tttggaacgg ggtcacagtt ttacgtagag
     3781 aatcgcattc attagctaat cttttaacgt tgatactagc catttttctt acactgtttc
     3841 ttgtttttga ctttttcttg ttaaaatatt tgccgcaatg gataaatgct ttgtttttct
     3901 gcgttcccct gatcttgatc tatctattta tcgtattcta caactttttg actgtctcat
     3961 tcttgtatca gttcaaccgg cctcgataca atcaagactt catcgttgtg ttaggcgctg
     4021 gattgatcaa tggagaaaca gtttctcctc ttctagccaa gcgaatcaat aaggcaattg
     4081 ctttttatcg ggctcaaagc cgtgctacat taaatccgcc catcttattg atgtctggcg
     4141 ggcaaggagc agacgaaaaa gtaccagagg ccattgcgat gaaacaatat gccatggaac
     4201 aaggaatccc tgaaagagac attcttgtcg aaaccaattc gacaaccact ttggaaaaca
     4261 tgctctattc aaaggaaatc atggatcagc aaatgaaagg gccataccgc gcgatcttta
     4321 gttccaacaa ttaccacatt tttagagccg gtctttatgc gcggcaagcc aaattgaaag
     4381 caaatggtat tggtgcaaaa acagctttct actacttgcc taatgcgttt ttaagagaat
     4441 acctcgccat cttgatgatg aacaaaaaaa gacatatgat catttgcgga ttgcttttcg
     4501 caggctccgc attcttatcg ctgattactt tacttttcta aaaaaacaag gtgtgacaaa
     4561 acatatgtca caccttgttt ttttctttca ttctcttaac tattggcaaa aagagtatcc
     4621 cttttttgac cgctacttgc tgagcagaat aaataccgct gttgcctgaa cacataaagc
     4681 tgatcaagca gatcataaag aagtatactg ccgcttgtga accgaacaat tcgatcccca
     4741 tcacaaagca ggcaatcggt gtatttgtcg caccagaaaa tactccgata aagcccaatc
     4801 ctgcaagaaa aggaacagaa acatggagca aaggcgctag tgctgcacca agcgttgcac
     4861 caatttcaaa caatggtgtc acttctcccc cttgatatcc cgcgcctaaa gacaaaactg
     4921 tgaagaataa cttcccaata aaatcataga catgtgcctg tccattaaat gattcttgaa
     4981 gtaacggaag actcaatccg agataacgtt gtgtttgcaa aatcaatgct gctgccacaa
     5041 ctacagctcc acctatcgta tttcgacaaa caggatgagg aatccattgt gcatatcgcg
     5101 cttttaaaaa gacgatcgaa cggctgaata cccaaccaat caaaccaaaa gcaatcccag
     5161 caacaaaaac tttgaagaac agcaacatac tccattcagg aatcacaccc atttggtaat
     5221 gtagatgaga aacaccatag ctttctgtca tgaaatttgc aaacagtgca gcaaagaagc
     5281 tagggaagat cgcttctgtt cggactttcc cgattgccaa cacttctaat ccaaataatg
     5341 ttccagctaa aggtgtgcca aatacagaac taaaacccgc actgattcca ctgataatca
     5401 agatttcccg ttctgattta tccaatcgga acaaacgtgc cgtattatct gctaacgctc
     5461 cccccatttg gactgctgtt ccttcacggc caacagatcc cccaaataaa tgggtagtta
     5521 tggttccaaa cagtgtaagt ggaataagcc gcaacgggat tttttcttct ccaccatttc
     5581 cttgatcgat cactaagttg tttccacgac tagaaagtcc gccgtgatac gcatagagat
     5641 aagcaaaaag cgcaccacta actggtagta gacaaaggag ccaaggatgt gatagtcgga
     5701 tatctgtcac aactgtcagg ctatttaaga aaaaagcaga caagctcccc atgaatatac
     5761 cgagaatcgc tgtgatcacc agccacttca gcatatacac taaaatgcgt actggactcg
     5821 gtaattgttt ttccataaaa aattcctcct caataaaaaa acgcctacta a
//
LOCUS       DACCQI010000143         5580 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCQI010000143 DACCQI010000000
VERSION     DACCQI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.150"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(1..761)
                     /locus_tag="IVO76_002533"
                     /pseudo
     CDS             complement(1..761)
                     /locus_tag="IVO76_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016609371.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(782..1159)
                     /locus_tag="IVO76_002534"
     CDS             complement(782..1159)
                     /locus_tag="IVO76_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7430468.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            1383..2576
                     /locus_tag="IVO76_002535"
     CDS             1383..2576
                     /locus_tag="IVO76_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7430469.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(2742..3164)
                     /locus_tag="IVO76_002536"
     CDS             complement(2742..3164)
                     /locus_tag="IVO76_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7430470.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            3754..4209
                     /locus_tag="IVO76_002537"
     CDS             3754..4209
                     /locus_tag="IVO76_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430471.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(4371..5543)
                     /locus_tag="IVO76_002538"
     CDS             complement(4371..5543)
                     /locus_tag="IVO76_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7430472.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
BASE COUNT         1609 a         1063 c         1006 g         1902 t
ORIGIN      
        1 tcactcgctt tgaaagatga cttacagcac cttctatgac taaagcagca cctacctgat
       61 tctcaattag cgcaaataca ttgaaaatca aattgtacgt tcctgtgatc agcaactgct
      121 ccttttttat gcctaaccag tcagcaaaca ttcgctgtat ttctggacgc tttgagatca
      181 aaagaggtac attcgcaaag tcttcttttg ttatcacgtc tttttttgct aaaacagaat
      241 tttctaaaat gagcaatccc catttttcct ttcttggtaa caaaatcgtt tcgtatttct
      301 ctgtatctac tggatctagc aaaatcgcta aatctaacag gcttttttct agtcactccg
      361 tgatatcatc acttgttccg ctgtggatat gaaattgaac gctgggataa tcacggatca
      421 acttttctag taatagtgcc aatgtattcg aattatcagc ttccacacag ccgatcgtaa
      481 atcggccgct gaataattgt ttttttctgt tctcgaattc ttgagtggtt tgattcgtta
      541 aatctaaaat ttcttctgct cggctttttt agaaaataac ctgcttctgt caagatcatt
      601 ttgcggtttt cgcgtacaaa cagttctgtt cctagttcct cttctaactc cttcagttgt
      661 cggcttaagg ttggttgtgt aagatgtaga acttctgcgg cttgagagat tgttccttct
      721 tctgcaattg tccaaaaata ttttagtaag cgtaactcca tagcgatgct ccttgatttt
      781 tttacttgat acattccatt ttatttctag ataacacacg atcatatttc ttgatttttc
      841 cttgtaaacg ttccaaacat tcttgcattt cagcaatttt tttcgtcaaa agttcacttt
      901 cttcaatcaa aagcttacgc cgctcaaata cagttgcttc tccttcgcta gataggcgaa
      961 tatattcaat caaagactcg atcgataatc cggcatttcg catacagatc gtaaagtcga
     1021 cccattttag gtcttcttct gtataatcac ggattccgcc ttttgtccga ttaactggtg
     1081 gaatcaaccc gattcgttca taatagcgca atgtatcggc cgaaatgccc ttttcttcac
     1141 tgacttgttt gatattcatc tgatcactct ttctcttaat ttcttgccac tttacttatg
     1201 tattttattt attctacaac tttaagtgta ctccaagtca atactcattt ctattaaaac
     1261 taagctggat tatctatatc ggttgtttgt gtattatttc aaataaaaaa gtcttgtttt
     1321 tttattcttt tttggatata cttttaacaa tttagggaaa ttttaggaaa aggggtataa
     1381 aaatgacaac gtatcagcga aagttattat tatcgacatc ggcgggaatt gctttagaga
     1441 atatggatat tatgttttta gctttttcat tatcttcaat gattacttct tttcatatta
     1501 gtggtacgca agcgggtttc atttcaacga tcactaattt ggggatgctc attggaggta
     1561 ttttttttgg catcatagct gataaatatg gacgtgtgaa agtattttct caaacggtcc
     1621 ttctgttttc gattgcttct ttattgatgt attttgcttc aaacatctac ttggtttatc
     1681 tctttcgctt tatcgcaggt gtaggagcgg gaggagaata cggggcgtgt atgtccttga
     1741 tttccgaaag tttttcaaaa aaacaaattg gacgagcttc ttcggttgct ggtattggtg
     1801 cgcaggtcgg tgcggctttg gctgcgattt tagctgctgt gatcattcca acgtttggtt
     1861 ggaagatgtt gtatgtcgta ggtgtactgc cggtgttgat ggtcttgttc attcgtcgcg
     1921 gcttgactga accaaaggaa ttcaaatctg tacaatctgc cgaaaagaaa accaaactta
     1981 gcagactgtt tgaatcaaaa catcttgctt ggcaaacatg tggccttagt atcatggtaa
     2041 ctgtccaaat tgccggctat tttgggttaa tgaattggct gccttcaatt atgcaaaatc
     2101 gattagggct ttcggtttct gggtcttcat tgtggatggt cagtactatt ttaggcatgt
     2161 ctttggggat gatgactttt ggcataatca tggataaatt ggggcctaaa ctagcctttt
     2221 ctttgttttt aatttgttca tccttatctg tctatctact tgtgttagcc catagccagt
     2281 ggagtttggt agcagccgct gtgatagtcg gctactttat caatggcatg tatggtggat
     2341 atggcgcagt gattagtagt ttgtatccaa cagaaatccg cgcgacggcg aataatttca
     2401 ttatgaacct tggacgagct gttggtggtt tttcttctat cgtcatcggt ttcttgatgg
     2461 atcattatag tttaactgcc gtaatcttgt ttttgagcgg tatttaccta gtcagtctgc
     2521 tggtcttgac aactcttact ggtgttacag cattaaggga aagcttgcag cattgatagt
     2581 tgagtctgta aatgacaaga aagaggtgtg gcaaaagctt gcatctgcgg taataagacc
     2641 agccaaatca aaaacatgag acactgtttc cgtttggctg gtcgtattac ttgatgcagg
     2701 gcagcttttt caactgctaa tttccttcta tctggcggct actattcttt gacgtccgtc
     2761 atttcaaagt actttactaa taacttagca taacgaaaaa ctgtggatac actcgtatat
     2821 tgagcgtctg cataatcttc caaatttttc tcaggatgtt gatacatgaa ttcaatcaaa
     2881 tgataattat atgattttct gtagaaatga agataaataa tgtagattgg gaagttctcc
     2941 tgcttttcca tagaaaagcg ccgattattc tcattcttca aaatgacctt atcaggatat
     3001 ttttggaaca attcttctaa atctgttaga taattcaaga tagacttact tgataccttc
     3061 aattcgtttt gtagttcttc tattgaacac caacgttctg tttcaagcag taaatgaatc
     3121 attttaattt tcttttgaga aacttttgtt aaaaaagtct gcatattttc tcctcctgta
     3181 tctactttac tatactacat acaagaaaaa aaacggatac aaatatcata ttgtcacacc
     3241 gtcaatagtg cataaacctg ttattatttc tctttttttc ctaagtattc tattgatatg
     3301 aatcatttaa tcgtttatta tataaatgtc aataatctaa taataaaata acgtacatat
     3361 tttattattg catacttaat aacagaacta tagcaaaaaa tcccgtgtat caaaacggga
     3421 ttagattaag tttcctcaat cactattcat aacacaatta ttataacaag taagtaaaca
     3481 attgaaaagg acatttattg aaaacggttg ttttttactt aaggaggaaa tttagtgaaa
     3541 aaattagttg cattgttttt ggtcgcagga agtttgttga gtgggtgtgg tgctactacg
     3601 tctactactc aaagcacaag ttcagctcag gcttccagca gcagtaccac gatctccgaa
     3661 agtacggcag attcatcgaa agaacaagaa caaattgaaa agaaaaaaga agaagagaaa
     3721 caaaaagcgg aaaaagctgc actagaaaaa aaagtgacag aagcggaatc tgctttgtca
     3781 caagctgaag caaatcctac aaaagaaaac tatgcaactg cttcagcagc tatccaagcg
     3841 ctacctgaaa acaaacaaga gttgacaagc cgtcttgctg caattgattc cacaatcaaa
     3901 gcaaaagaag cagaagaaga acggaaaaag caagaagaaa ttgcacgaca acaagcagaa
     3961 caagcgcaac aagaagcaat cgcacagcaa caagcagaac aggcgcgaca gcaagcagca
     4021 gctgaacagg cctctcagca ggcacaagcc gcagctcctg agcaaaataa tgaacaaact
     4081 gtctatgtca caccgaccgg ttcaaaatac catacacata aatgtggaaa tggtacgtat
     4141 acccctgcca ctttaagcga agctcaagct cgtggtttaa ccccttgctc caaatgttat
     4201 ggaaactaat tcaaaagtta tactataata ataaaattct tctgaaacat aaaaaaatag
     4261 aagctggcgt tccagatagt aaaccaaaaa tagcagttta cacgtccatt tcgtcagctt
     4321 ctctcttttt tcatttagtc tgttccctct gcttttttgg acatcttcat tcattctttc
     4381 tcaatcaaat agtacacctc tttttctttt cgcagtttat atcttccttt tttcaatttc
     4441 attgaaaact gtttcgaatg tttatgtttt acatggattt taagcaggag ctcctcatgt
     4501 tgcaagcgat agttcctgcc ttttaaatca gaaaatctca ctagtccgta ttcatcagaa
     4561 gctttcacaa atggaacttt ttctctagta atatagtgtt tgccgttgag cgtttttaca
     4621 gaaaagacca ttccttcgat tggtcgtccg tttgtgttct ttacataaaa agataaagga
     4681 tagtttcgct tagcgatggc caaagaatct aaataagcga tgagtagcca aactaagccg
     4741 attgatccaa gtagtatacc gataaaccaa ataaaaaatt tccatttttt ccattgatca
     4801 attccttgga tcgctttttt tgcctctttt gcttgataag gaatacgatg ccccgtcaca
     4861 agcagtcgat gtgtattgac catataagga gtacaggtaa gcaaagtcac gagatctctg
     4921 cctttttgga tacccaaaga atctacttca tcaggtaaga ccacttggat tttttctact
     4981 tgatacgcca atgtctttcc attgatctgg atgaaaaact ggtctccttt tttcaactta
     5041 ggcaaatctg taaataattt tgcttctggg agtccacgat gcccagaaag aacggcatgg
     5101 gtgtttttcc cgccagtcgg ataagaactt tcttccaata atgacgttcc tttttgcaaa
     5161 aatatttccg ttgtctgatc gaatataggt aagctgacat tgatttttgg gatttcgatg
     5221 actccaagca tatgttccat aaaaaaggca ttactaggta aatcttttaa tgcttttgca
     5281 tcaactgctt ggttgaaact gcctatccct ggtgatacgt tttgctctgc caactgttgg
     5341 tttttctttg tcattttttc ctgctgtttt tttatttcct ctgaattctt attcgatgct
     5401 tgcttttgat aatggatgat catttgctgg gcaagaaaat cattcagtga atcttctaca
     5461 aaaggataag aaaagatccc gatcccaacg actaaaatga ttgccatgaa gatgtccagt
     5521 attcttattc tgcttttatt catcatgtcc acctattttc taatatgtat atgaagaggt
//
LOCUS       DACCQI010000144         5573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCQI010000144 DACCQI010000000
VERSION     DACCQI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.151"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(118..432)
                     /locus_tag="IVO76_002539"
     CDS             complement(118..432)
                     /locus_tag="IVO76_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7430473.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(446..706)
                     /locus_tag="IVO76_002540"
     CDS             complement(446..706)
                     /locus_tag="IVO76_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7430474.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            876..1190
                     /locus_tag="IVO76_002541"
     CDS             876..1190
                     /locus_tag="IVO76_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7430475.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1192..2847
                     /locus_tag="IVO76_002542"
     CDS             1192..2847
                     /locus_tag="IVO76_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7430476.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2930..5065
                     /locus_tag="IVO76_002543"
     CDS             2930..5065
                     /locus_tag="IVO76_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7430477.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5240..>5573
                     /locus_tag="IVO76_002544"
     CDS             5240..>5573
                     /locus_tag="IVO76_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430478.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
BASE COUNT         1890 a          963 c         1133 g         1587 t
ORIGIN      
        1 acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga agtagcttct
       61 tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct ctactgacta
      121 aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga cacctgctgg
      181 tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac gcatagcatt
      241 ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg cagagtctag
      301 ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg gagaagcttt
      361 tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat caatatggaa
      421 aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc gcttgttcta
      481 gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt ttcagattat
      541 ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt aattgagtga
      601 tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga atttgtccct
      661 ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca cattctcctc
      721 tatttttata attagataat accccttatc ggtattttat gcaaatataa aaagtagtga
      781 aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa tataccccat
      841 gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt caatcaataa
      901 aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg ttacttgatg
      961 ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat gttccattac
     1021 aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag
     1081 ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat gtagtgaatg
     1141 tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa gatgaaaatc
     1201 gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg ccgattaatg
     1261 gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc
     1321 ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca
     1381 gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt gacagccatc
     1441 tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat tgaacagtac
     1501 gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc agggataaca
     1561 gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt gatgcatgca
     1621 ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg attggaaatg
     1681 gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc accacatatt
     1741 cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc tcatcaagga
     1801 gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa acgattgcga
     1861 ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg tgttcgtcct
     1921 gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg tatcctagta
     1981 gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc tatcgttgta
     2041 aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc caatcgtcaa
     2101 ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg cggtattgga
     2161 acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag tgaaagaaca
     2221 gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga tcatgcaagc
     2281 tattatccag aagcaacaga tattttactg aagttgatct tccatccaga gactggcgag
     2341 atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga tattttagca
     2401 acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt gacgtatgca
     2461 ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc aatgaacatt
     2521 gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact agcaaaagga
     2581 aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt caaggaagcc
     2641 aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag ccaacaagaa
     2701 tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat cttgaagcag
     2761 tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac tgtccgacct
     2821 gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc gactcttaaa
     2881 agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta tggtcaacac
     2941 acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa ttgataaaaa
     3001 aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga
     3061 aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg atactttttc
     3121 gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga aaaacgcaga
     3181 aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa aagtcgttgt
     3241 agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa atttcacaga
     3301 tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac ttttttacaa
     3361 taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata cggttattaa
     3421 agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg atacgtctgt
     3481 agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca atgaaatgaa
     3541 ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg tgaaaagtca
     3601 aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca tgcagcgtat
     3661 tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc tgattctatc
     3721 acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag gtgaaagaaa
     3781 tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg aaatagaagg
     3841 tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga cagaacgttt
     3901 gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt tagatcatcc
     3961 gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga
     4021 aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc aagcaacgaa
     4081 aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt
     4141 gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg gtaaattgat
     4201 ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac aaaaaaaata
     4261 tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc agttgtttga
     4321 aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac gtactgttga
     4381 tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc
     4441 atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat taggtctatt
     4501 gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc aggcaatctt
     4561 taattcttcc actacttttg ctgatattat gcgttgtttc gatgactacc atctgccgat
     4621 ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag agcttttcaa
     4681 aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc aaggaaaaga
     4741 acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag ctgagatttt
     4801 ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg aaacccaaac
     4861 gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa tcattccttt
     4921 gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg aaaacgatgg
     4981 agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa tgaaaagcat
     5041 tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac aactgataaa
     5101 aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa
     5161 tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac gtcgtaacaa
     5221 aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat caattttggt
     5281 gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa cgttagtggc
     5341 tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc cattaacgag
     5401 accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga aaatgctgta
     5461 tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct catttgtatt
     5521 gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg aat
//
LOCUS       DACCQI010000145         5466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.152, whole genome shotgun sequence.
ACCESSION   DACCQI010000145 DACCQI010000000
VERSION     DACCQI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.152"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            133..1107
                     /locus_tag="IVO76_002545"
     CDS             133..1107
                     /locus_tag="IVO76_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7430479.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="IVO76_002546"
     CDS             1089..1550
                     /locus_tag="IVO76_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7430480.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="IVO76_002547"
     CDS             1600..2064
                     /locus_tag="IVO76_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7430481.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="IVO76_002548"
     CDS             2078..2827
                     /locus_tag="IVO76_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7430482.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="IVO76_002549"
     CDS             2827..3657
                     /locus_tag="IVO76_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7430483.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="IVO76_002550"
     CDS             3657..4304
                     /locus_tag="IVO76_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7430484.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="IVO76_002551"
     CDS             complement(4402..5178)
                     /locus_tag="IVO76_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7430485.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="IVO76_002552"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="IVO76_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          739 c          973 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DACCQI010000146         5399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCQI010000146 DACCQI010000000
VERSION     DACCQI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.153"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            204..1478
                     /locus_tag="IVO76_002553"
     CDS             204..1478
                     /locus_tag="IVO76_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7430486.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1532..2959
                     /locus_tag="IVO76_002554"
     CDS             1532..2959
                     /locus_tag="IVO76_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7430487.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2972..3844
                     /locus_tag="IVO76_002555"
     CDS             2972..3844
                     /locus_tag="IVO76_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7430488.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3939..4934
                     /locus_tag="IVO76_002556"
     CDS             3939..4934
                     /locus_tag="IVO76_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7430489.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
BASE COUNT         1856 a          749 c         1048 g         1746 t
ORIGIN      
        1 taaaaaatag tgccattaaa aaagaaaagc gacacaccaa taaaagtcat atcctgtgac
       61 attatatggc attatccaaa tagaataacc ggttgacatg taagcggatt tcttttataa
      121 ttttaggagt acaagtcctt ttctaaaaat gttaaccggt tatcatgaaa ggggatacaa
      181 atatatcgtt ggaggataaa aatatgtcaa aagttaacgt atttaaaaat caatcttatt
      241 tacaaagttc agctacatta ttactatttt ttgcttcttg gggtgtttgg tggtcatttt
      301 ttcaactttg gctaacatct gaatcaaatg gtttagggtt atctggcagt gctgtaggaa
      361 cagtattctc ggcaaattcg ttagttacct taattttgat gtttatttat ggaacattac
      421 aagataaatt gtatattaaa cgaaatttat taatttttgc ttctgtatta gcgacacttg
      481 ttggaccatt ttttatatgg atatatgggc cattgctaga taacaatttt aatttaggca
      541 ttattatggg agcgctattt ttgtcagctg gatatttagc ttctgtagga gtttttgaag
      601 ctgtgtcaga aaggtttagt cgtttatttg gctttgaata tggacaagca agggcgtggg
      661 gatcatttgg ttatgccttg gtagcgcttt tggcaggatt tttatttgta aaaaatcctc
      721 atttaaactt ttgggcggga tctttctttg gttctttact attgttaaat ttattatttt
      781 ggaaccctaa agttgaacgg gaagcaaatc aaaattttaa tcaagaacaa gctgaatcaa
      841 atagtattcc ttctttaaaa gaaatgtttg atctaatgaa actgcctcaa ttatggacga
      901 taatcatctt tattgttttt acatggacat tttatacggt attcgatcaa caaatgtttc
      961 cgggatttta tactggtttg ttttcaacat cagctaatgg tgaaaaaata tatgggacat
     1021 tgaatgctat tcaagtattt tgtgaagcgt taatgatggg aattgttcca atcattatga
     1081 gaaaattagg ggttcgaaat actttgttat taggtgtaac cattatgtgt gtacgaattg
     1141 gattgtgcgg gtttgcctcg acaccattat ctgtttcatg cataaaaatg ttgcatgctt
     1201 tagaagtacc attatttaca ttaccaatgt ttcgctattt tacacttcat tttgatacaa
     1261 agctatcagc aaccctctat atgataggat ttcagatagc tgctcaaatt gggcaagtga
     1321 ttttatcaac accattggga atattaagag acaacgttgg ctatcaacca acatttaaaa
     1381 ttatttctct tattgtatta ctagcaggca tatatgcatt ctttattctt aaacaagatg
     1441 atagagatgt tcaaggggat ccatttattc gaggataatg gaaaccaaca gaataaaaaa
     1501 caattttatc tagaaagtga gcgaatatat tatgacaaaa agagctatag aattaacaaa
     1561 tacacgttat cgtttgggat atcatgtgtc tgcaccatca ggatggatta atgatccaaa
     1621 tggattttgc tattttgatg ggtattatca tgtgttttat caacatcatc catatagtgc
     1681 tgaatgggga ccaatgcact gggcacatgc tagaagtaag gatttagttc attgggaatc
     1741 tttaccactt gctttgactc ctggagatca agaagatgaa ggtggttgtt tttcaggaag
     1801 cgctattgaa aaaaatggtg tgttgtatct gttctataca ggacatcact attatggaga
     1861 caatgatcca gatcattttt ggcaaaatca aaacatggcg tatagtacgg atggcattca
     1921 ctttactaaa tatgaaaaaa atcctgtaat agcaaaagca ccagaagata acacacatca
     1981 tttcagagat ccaaaagtat ggaaacacga tgaaaaatat tacatggttt tgggtagtca
     2041 agaaaaagat ggattaggtc gtgctattgt ttatcagtcg ttagatttat taaattggga
     2101 ttacattgga ccaatttcta aagcgaagga agtacttaca gaaggattta tgtgggaatg
     2161 cccagacttt tttgaattaa atggaaaatc tattctttta ttatcccctc agggaattga
     2221 agcaaatgga aaagattatt taaatctata tgaaacaggc tactttatcg gtgattatga
     2281 ttatgagaca gcaaaattca ttagaggaaa tttttatgaa ttagataagg gacacgattt
     2341 ctatgcaaca caaacaactt tatctccaga tggtagacgt attgttatgg cctggatgga
     2401 tatgtgggaa agccctatgc ctgaaaaagt agacggatgg gcaggagcat taacgcttcc
     2461 tcgagaatta gagttaattg gagatcgttt gtatatgaaa cctgtgagag aattagaaca
     2521 acttcgagtt ggcagcggcg ttgaaacgac agttaatttg gcaggagaaa taataatcgc
     2581 tcaagatgct tcattcaaag aagctctaat tgatgtacta ttgactaaat caaaaaatca
     2641 agaagtcaaa ttttcattaa aaacaaaaga tgaagagtta atcaatattt tttattctaa
     2701 ggataaaaat gaatgtacct taactcgtaa cggcaaaaat gatccaaggt atggaaccat
     2761 tcagatgtgt gatagattat cacttcgagt gtttattgat aaaagttcgg cagagatttt
     2821 tattaatgat ggcgagcttg tttttacaga gcgttattat acagaaaatc aaccaagtat
     2881 gcatttgagt gttagtgaag aagcttttgt ctcgagtaca atttatcatc taaaaaaaga
     2941 tgcaatttct tacgagtaag gaggttttat gatgttaatt ggtagcatag aagcaggtgg
     3001 gactaaattt gtatgtgccg tgggagacga ggattaccga gtacaagacc aaataatttg
     3061 tccaacgacc acccccaggg aaacattgaa aaaaactgtt gactatttta aacagtttga
     3121 tataaatgca ttaggtattg catcatttgg tccaatagaa attcgaaaaa attcaccaaa
     3181 atcaggatat attacgtcta caccaaaggt gggatggcga aatgttaatg tgttaggtta
     3241 tttacaagat gagctgaatg ttccaatgtc ttggacaacg gatgtgaacg gatcagctta
     3301 tggagagtac atcatgtcca ctttatcgaa tgaaaaaata aattcattag tttattacac
     3361 cgttggtact ggcgttggtg gtggagctgt tgttgacgga aaactattag gatcgtttgg
     3421 tcattcagaa atgggccata cgtttgtaaa acgccatcct gatgatattg attttaaagg
     3481 aatttgtcct tatcatggtg attgtttaga aggcttagtt gcgggaccaa cgtttgaagc
     3541 acgttttgga aaaaagggtg aagacgtttc tttaacggat cctatttggg atgtaatggc
     3601 ctattatcta gcacaagctg tgattcaaac aacattgatt atacgaccgg ataaaatagt
     3661 atttggtgga agcgtagtaa atgaaaaatt gttagaaaaa attcgtgtta atgttaataa
     3721 attattggat gattacgtgg aaataccacc gttaagtaaa tatattacgc gtcctttaat
     3781 taaaaataat ggatcggcaa cactaggaaa ctttgctttg ggcttgaatc aattacaaga
     3841 ataaaacata gaaaaaatat cgattatcag gtatagtagt tctatagagt tctttttttc
     3901 gatagttaat tattgcatta tttaggagag acaagagaat gcgcccaaaa ttagaagatg
     3961 ttgccaaaag agcaaatgta tcaaaaacaa ctgtttcacg tgtattaaac aatcgtggct
     4021 atttaagtca aaaaacgatt gataatgttt ataaggctat agaagaactc aattatcaac
     4081 cgaacgttgt agcgcgtcaa ttatttcaaa agaaaactaa cattgtcggg ttactatttc
     4141 ctacagtagc caatcctttt tttagtgaac tcgttgaggc attggaaaaa aagttatatg
     4201 aaattggtta taaggtatta ataggcaatt cgatgaataa taaagaaaag gaaacaaatt
     4261 atttaaatca gttattgtct gatcaagtag atggtttaat tgttggtaca cataaccaag
     4321 gaattcagga atataaatat cagaatttac cgattgttgc aattgatcga gtgatgaatg
     4381 aagatattcc agttgttgaa tccgataact acaatggtgg aaaacttgca accaagttat
     4441 taattgcaca aggtgcaaaa aatattattc atacaaacgg tccaattgac ttacagactc
     4501 cggctaatcg tcggagacta gcctatgaag atactatgaa agcatatcag ttaattccac
     4561 gaactgtgac attagatttc aatatttctt atgtaaagaa aaaacagatt tttttccaaa
     4621 tgtttgagga ttatcctaaa atagatggta tttttgcttc aaatgacatc gatgcggccc
     4681 taatattaca agtagcaaaa gaaaaggggt taaatgtacc agctgattta ttagtagtcg
     4741 gatatgatgg tactttaatg actcggagta tattaccaga tctgacaaca gtaatacaac
     4801 caattaatga tatagcagac acagctgttg ctattttgat gaaacgaata aataaagaag
     4861 aaacaaaaaa ggaatatatt ttacctgtca cattatggat ggggaagaca tcagtaaaga
     4921 aaaagaacaa ttaaatttat aaagactatc aatgataatt tcattattaa ttcgtgatat
     4981 aaaaaattgt taaatctctt ataatagact atgatacaaa atcatgcttt taatctgtgt
     5041 agatgttgcc aaatatttga aagacataga acttgtcaga aaaaccttaa caattcctca
     5101 atgggcggat aagctaggca aaagggctgg cataaatttt tcagtccttc taacagaatc
     5161 aattgcagat aaagcagata acattcttcg ttctggaaga aataattaga actcaaaagg
     5221 gtcagaaact taaaagtttt tgatcccttt tttattcctc atttcatagc ttttttagta
     5281 gctcaattaa ttaggtcaga aagttacttt tttaaaagga caccagagta taccctgttg
     5341 ggctcttggt gttctattga atgtaaatga gtgtcatttt tatatttaat tggcacagt
//
LOCUS       DACCQI010000147         5334 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCQI010000147 DACCQI010000000
VERSION     DACCQI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.154"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1592
                     /gene="hflB"
                     /locus_tag="IVO76_002557"
     CDS             <1..1592
                     /gene="hflB"
                     /locus_tag="IVO76_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7430490.1"
                     /translation="AKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            1809..2696
                     /gene="hslO"
                     /locus_tag="IVO76_002558"
     CDS             1809..2696
                     /gene="hslO"
                     /locus_tag="IVO76_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7430491.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            2697..3701
                     /gene="dusB"
                     /locus_tag="IVO76_002559"
     CDS             2697..3701
                     /gene="dusB"
                     /locus_tag="IVO76_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7430492.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            3812..5308
                     /gene="lysS"
                     /locus_tag="IVO76_002560"
     CDS             3812..5308
                     /gene="lysS"
                     /locus_tag="IVO76_002560"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7430493.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
BASE COUNT         1841 a          946 c         1238 g         1309 t
ORIGIN      
        1 aagccaaaga agcagacaaa aaagctaatc gcgtacgttt ttctgatgta gcgggtgcgg
       61 aagaagaaaa acaagaacta gttgaagttg ttgaatttct gaaagatcct cgccgatttg
      121 ttgaattagg tgcccgtatc ccggcaggtg tacttttaga aggacctcca ggaacaggta
      181 aaaccttatt agcaaaagct gttgccggag aagctggagt accgttttac tcgatctcgg
      241 gttctgattt cgtagaaatg tttgtcggtg ttggagcaag tcgtgttcgt gatttgtttg
      301 aaacagcgaa gaaaaacgca ccagctatca ttttcattga tgaaattgat gcggtaggtc
      361 gtcaacgtgg tgccggtatg ggcggcggac atgatgaacg cgaacaaaca ttgaaccaat
      421 tgcttgtaga aatggatggc ttcgacggaa atgaaggagt aatcgtcatt gctgcaacca
      481 accgttctga tgtactagac ccagcgttac tacgtccagg ccgttttgac cgccagattt
      541 tagttggacg tccagatgta aaaggtcgtg aagcaatctt gcgagtccat gcacggaata
      601 aaccgatggc agatgacgtt gacttgaaag ttgtggctca acagacacca ggttttgccg
      661 gagcagattt agaaaatgtc ttgaatgaag cagcgttagt agctgctaga agaaataaaa
      721 agaaaatcga tgcttctgat atcgatgaag cggaagatcg cgtgattgca ggtccagcta
      781 aaaaagatcg cgtaatcagc aaaaaagaac gcgaaatggt tgcttatcac gaagctggtc
      841 atacgattgt cggattagta ttgagtcgcg ctcgggtcgt ccacaaagta acgatcatcc
      901 ctcgcggacg cgccggcgga tatatgatcg ctttaccaaa agaagatcag ttcttgatga
      961 cgaaagaaga tatgtttgaa caaatcgtag gcttacttgg tggacgtaca gctgaagaaa
     1021 tcatcttcaa tgtccaatca actggtgctt ccaatgactt tgagcaagcg acagctctag
     1081 cacgaagcat ggtaacagaa tatggaatga gtgatcgttt aggtcctgtt caatatgaag
     1141 gaaaccatca agtgtttgtt ggccgcgact atggtcaaac aaaagcttac tcggaacaag
     1201 tcgctttcga gattgatcaa gaagtacgaa aaatcttgat ggaagctcat gataaagctc
     1261 gagaaatcat cgaagctcat cgtgcacagc ataaactgat tgctgaaaaa cttttggaat
     1321 tcgaaacatt ggatgccaaa gcaatcaagt cattgtttga acacggtgtg atgcctgaag
     1381 gtactgagtc aaatgattat ccaagcgaaa aagaagcgaa aacttttgaa gaagcaaaac
     1441 gtgctttgga agaaaaagac gctgaaaaac aagcggaaga aaaacgtgat tttgaagaag
     1501 caaaacatga actgaatgag gaaaataaag cagaacatga ttcagattct caacaatcag
     1561 aagataatca ttcagacgat caatacaaat aaaaaaatat aggtcaattg atctgatatg
     1621 actcccgaaa accggaacaa aaaccggttt tcgggagatt tctttattta agttgtaaat
     1681 agggtgatgt cctgttttct agatgattct gaattatttt taaaagaagc attccaaagt
     1741 tgaaaaaagc agtagttgct agtatgattc actatgagtt taaaaacgga aaaaggagta
     1801 cataacaaat gaaagattat ttggttaagg cacttgctca cgacggattc gtccgagcat
     1861 acgccgttca agcaacaaat accgtagcgg aggcacaaag acgtcatgat acttggcaca
     1921 cctcctcagc tgctttggga cggacattgg tcggtagtct gctacttggt gcgacgctca
     1981 aaggagaaga caaactaact gttcgaatcc aaggcgatgg tccagcaaca ggaattgtag
     2041 tagatgcaaa tggaaaggga gatgtgaaag gttacatcaa aaatccgcat ctcagtcttc
     2101 ctctaaatag tgaaggtaaa atcgatgtgc ggggagcagt tggaacacaa ggaatgttta
     2161 ctgtcacaaa agacttagga ttaaaagaac ctttttctgg gcaaacgccg attgtttctg
     2221 gagaaatcgc tgaagatttt acttactatc ttgctatctc agaacaaatc ccttcagctg
     2281 ttggactgag tgtattggtc aatactgacg acagcatcaa gacggcggga ggattcatga
     2341 tccaaatcat gccaggtgcc gatgaagaaa caatcagcaa gatcgaagaa caattaaaaa
     2401 cgacaccaag aatctctacc ctcttagatg aaggacagac accagaagaa atattgaaag
     2461 gattgcttgg tacagaagaa ttgaacattt tggaaaaaat gcctgttcaa ttcaaatgtg
     2521 actgttcaaa agaaaaattt gcagaagcaa ttatcgcgtt aggtgcagaa gaaatccaag
     2581 caatgataga cgaagatcac ggtgcagaag cggtttgttc tttctgtaat aacaaataca
     2641 agtatagcga agaagaatta gatggattga agaaagaagc cttaggaggg atttaagtgg
     2701 aacacacatg gaaaattggc aatgtggaaa tccctaatcg tgtcgttgtt gcaccgatgg
     2761 ctggaataac aaattcagca tttcgagtca ctgtcaaaga atttggtgcg ggcttagtcg
     2821 tatgtgaaat gatcagtgac caaggtattc atttcagaaa caaaaaaact ttagagatgc
     2881 tccacattga agaaacagaa catccattaa gtttacagat atttggcgga aataaagata
     2941 gtctagttga agctgctcaa tttgtcgaag aaaacacagc tgccgatata atagatatca
     3001 atatgggctg tccagtaaat aaagtaatca aagcagaagc aggagcaaaa tggcttttag
     3061 atcctgagaa agtatacgaa atggtccaag ctgtttcttc agcagtaaat atcccagtaa
     3121 caatcaagat gcgtattggt tgggatgaag agcacgtctt tgctgtagaa aatgcattag
     3181 cagcacaatc agctggagca agtgccatcg ctatgcatgg acgtacacgg gttcagatgt
     3241 atgaaggaaa agcggactgg gaagtattga aagaagtgaa aaaacatttg acgatcccat
     3301 ttatggggaa cggggatgtc aaaacgccag aagatgcaaa atggatgctt gactatgtcg
     3361 gtgcagacgg tgtgatgatt ggtcgtgcag cgctgggcaa tccatggatg attcaccgca
     3421 cacagcatta tctcgaaaca ggagaactga ttcctgaacc aacaccagcc gaaaaaattg
     3481 ctacagctaa acttcattta caacgcttag cagatctcaa aggagaaaaa gtagcttgta
     3541 gagaattcag aacacatgcg gcttattatt taaaagggat tcctcgagca gcgaaagtga
     3601 aagtagcaat caatcagaca gaaaaacagc aagaaatcaa tgacctgctg gatcaatttg
     3661 tagaaaaagc ggaaaaagta ctattgaaac aaacgttgtg atttctaagt ttttcgtgaa
     3721 ttttcaagcc gaaattccaa gttcggcttg ctttttttgt gtttcattgg cattatagga
     3781 aagagattaa catgagaatg gaggaacttt tgtgacacag gaacaacaac atcaagtaga
     3841 agatttaaat gatcaaatgc ttgtacgtcg tcaaaaaatg gaacaattgc gtgaagatgg
     3901 aatcgatcca tttggaaaac gatttgaacg cacgcacaac tcagaagaat tgcatgaact
     3961 atttgatcca agaacaaaag aagaactagc agaaatggga ttgaccgcta gtgtagctgg
     4021 tcgtatgatg actcgccgag gaaaagggaa aactggattt gcgcatctac aagatcgtga
     4081 aggccaaatt caaatttatg taagaaaaga ccaagtaggt gatgaggcct atgaagtatt
     4141 caaacatgca gaccttggag atttctttgg tgtaactgga caaatcatga aaacagacac
     4201 aggcgaagtt tctatcaaag cttcagaaat cacgattttg tcaaaagcac ttcgtccact
     4261 gccagataaa taccatggct taacgaatat tgaacaacgc tacagacaac gctacttgga
     4321 tttaatcagt aatcgcgaaa gctttgaccg atttatgaaa cgaagccaaa ttatcagcga
     4381 aatccgtcgc tatttagatg gcaatggcta tattgaagta gaaacaccag tattgcacaa
     4441 tgaggcaggt ggagctgccg cacgcccatt tatcacgcat cataatgcac tggacattga
     4501 tttatatttg cgtattgcgc tagaacttca tttaaaacga ctaattgttg gcggaatgga
     4561 aaaagtttat gaaatcggcc gcgttttccg taatgaagga attgatacta cacataatcc
     4621 agaatttacg atgttagaag catacacagc atatactgat ttcaatgatg taatgaatct
     4681 gacagaaggt attatccgta acgcagctga aaaagtacta ggaacagcaa aaatcacata
     4741 tgatggacaa gctgtcgact tagaaagtga ctttaaacgc atccatatgg tagatgcaat
     4801 caaagaacaa actggtgtag acttctggca agaaatgaca ttggaagaag cattagcttt
     4861 agccgaaaaa cacaatgtag aagtaaccga agctatgggt gtcggacatg ttatcaatga
     4921 attctttgaa acatttgttg aagaaacatt gacacaaccg acgtttgtat acggacatcc
     4981 agtcgaagtt tcgcctttag cgaagaaaaa tccagaagat cctcgattca cagatcgatt
     5041 cgaactattc atagtggggc gcgaatttgc taatgccttt acagaactga acgatccaat
     5101 tgatcaaaga gaaagattcg aagcgcaaga aaaagaacgt gaactaggaa atgacgaagc
     5161 tcacggtgta gacgaagact tcttggaagc cttagaatac ggtatgccgc caactggtgg
     5221 tctaggcatc gggatcgaca gattagtaat gttgctaact gatgcacaat caattcggga
     5281 tgttttgcta ttcccaacaa tgagataatc gaataaaagc tgtctaagga attc
//
LOCUS       DACCQI010000148         5281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCQI010000148 DACCQI010000000
VERSION     DACCQI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.155"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(42..1190)
                     /gene="nagA"
                     /locus_tag="IVO76_002561"
     CDS             complement(42..1190)
                     /gene="nagA"
                     /locus_tag="IVO76_002561"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7430494.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1266..2102)
                     /locus_tag="IVO76_002562"
     CDS             complement(1266..2102)
                     /locus_tag="IVO76_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430495.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2286..3503)
                     /locus_tag="IVO76_002563"
     CDS             complement(2286..3503)
                     /locus_tag="IVO76_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7430496.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3886..3969
                     /locus_tag="IVO76_002564"
     tRNA            3886..3969
                     /locus_tag="IVO76_002564"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3920..3922,aa:Leu,seq:gag)
     gene            complement(4209..5207)
                     /locus_tag="IVO76_002565"
     CDS             complement(4209..5207)
                     /locus_tag="IVO76_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430497.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1637 a         1076 c          837 g         1731 t
ORIGIN      
        1 caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc
       61 acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca
      121 tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc
      181 agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta
      241 attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt
      301 ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg
      361 gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt
      421 cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct
      481 aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg
      541 aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta
      601 gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct
      661 tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct
      721 tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct
      781 ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt
      841 gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac
      901 gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact
      961 ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta
     1021 tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca
     1081 ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt
     1141 ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct
     1201 ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac
     1261 accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct
     1321 gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg
     1381 aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac
     1441 gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa
     1501 aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa
     1561 tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc
     1621 agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct
     1681 tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg
     1741 agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa
     1801 gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt
     1861 tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc
     1921 gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta
     1981 ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc
     2041 attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc
     2101 ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt
     2161 cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc
     2221 cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt
     2281 taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc
     2341 aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat
     2401 ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca
     2461 tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt
     2521 ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt
     2581 tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg
     2641 gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc
     2701 agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact
     2761 cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca
     2821 ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt
     2881 tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat
     2941 tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc
     3001 cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg
     3061 caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag
     3121 tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg
     3181 atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt
     3241 ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag
     3301 tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca
     3361 aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc
     3421 atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg
     3481 ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt
     3541 tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac
     3601 ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc
     3661 atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag
     3721 cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc
     3781 cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca tttttgagct
     3841 tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa
     3901 ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca
     3961 tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt
     4021 tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta
     4081 aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg
     4141 ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt
     4201 tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat
     4261 ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc
     4321 attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc
     4381 catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata
     4441 actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat
     4501 agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt
     4561 ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat cattgccctt
     4621 ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg
     4681 tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat
     4741 gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac
     4801 ggctttccaa atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat
     4861 tcaggaaacc atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa
     4921 gagaaaatta attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt
     4981 ccattgacaa tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact
     5041 tgttcttgat tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt
     5101 atttcttctc ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact
     5161 ggccactgat agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt
     5221 attaaacagt atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca
     5281 t
//
LOCUS       DACCQI010000149         5216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCQI010000149 DACCQI010000000
VERSION     DACCQI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.156"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..314)
                     /locus_tag="IVO76_002566"
     CDS             complement(<1..314)
                     /locus_tag="IVO76_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7430498.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ET"
     gene            complement(678..3344)
                     /locus_tag="IVO76_002567"
     CDS             complement(678..3344)
                     /locus_tag="IVO76_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7430499.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3685..5136
                     /gene="cls"
                     /locus_tag="IVO76_002568"
     CDS             3685..5136
                     /gene="cls"
                     /locus_tag="IVO76_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7430500.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         1514 a         1105 c          919 g         1678 t
ORIGIN      
        1 tatgtctcat ttgattggat aaattcttct aacgactgtt tgataaagga aacatttggc
       61 aactcatttt tttctgcttc tttttttgca ttttctagca ttcctgaaga aaaatcgatc
      121 aaatcatagc gattaatttc ttttgctaag gaaagaaatc tgccagatcc tccagcaacg
      181 tctgctgctg tttcagcagg caggactttc tccgttttca gataattttt tacatcttca
      241 gcaatagtta cttgcgattc ttgctggatt tcataatatt cttcagcaaa ttcatcccat
      301 tctttttcat tcatttgatt tcctctaatt ttttgttata tgttttcact tttcggaata
      361 aatcattctc ctcttttatc tattctatct cttttgatga ataataaaat gattggcttt
      421 ccgtctatca taaataaaaa aagggactgt gaccaaagtc gtgtcaaagt ccctctttcc
      481 gaataaacag tgtccaatca gatgaccctc agtattagct caaaatgctg attttacaac
      541 ttcttcctta aacggtgttc tcctgccaac tcttcggtca caagtcacaa tattcaacaa
      601 acatgagaag ctgtttgttg aaattgcacc ttgtgactca gaattaaacg gcaggctcac
      661 aacctcgttg attcgtttta tttatttcct tgtactcgta caatgatcaa tttagtgatt
      721 tccatcaaaa caattgtcac aagtgcgatt cccatacatg taaagacttc tgtcaatcca
      781 aatgctgatg ggatagagaa gacttctttc agtcctggga tcattgttaa gcagtaaagc
      841 gcgctacata caactacagc tagtaagaca cttctgttag agaagaaccc tgcttggata
      901 gctgtttgtg tgtttgaacg tgctgggaat gtttgcaatg tccgacacca aatcaatgtt
      961 gaaaatgaca tggctacacc gaattcaggt gaatgctgca tgccaagcca ttgagcaaag
     1021 ataacagcaa tcccaatcag cacaccacga tacgttacag aaatcaatgt tttgtccgta
     1081 aagattcctg aatttggatc tcttggcggg cggttcatcg tagcaggttc agctttttct
     1141 gtacctaatg caattgctgg cagtgaatca ttgactaaat tgatgaacaa cagttgtaat
     1201 gctgtaaatg gattggacca gtcagcaact aaggcaaaga tgatcgcaat gatcgcacct
     1261 aaatttcctg caaatagata agaaatagct ttcttgatat tatcaaacac attacgtcct
     1321 acttctactg cgctgacaat agaagcaaag ttatcatccg tcaataccat cgctgctgca
     1381 tcttttgcca catctgtacc cgttcccata gcaattccga tgtctgcttg tttcaatgct
     1441 ggtgcgtcat ttacaccatc acctgtcata gctgagatat gacctttatt ttgccaagca
     1501 cgaacgatac gaattttatt ctcaggtgaa acacgtgcat aaacagtgac tttttctaaa
     1561 acttcgttta gttgatcttc tgtcaattca tctagttccg tcccggtcaa agccaggttt
     1621 ccttcttcaa aaatcccgat ttgttttgcg atagcaacag ctgttgtttt gtggtcacca
     1681 gtaatcatga ttgttttgat tcctgcactt cttgcttctg ttaccgcctg agtcacttca
     1741 gcacgtggag gatcgatcat agcaagcaaa ccaacaagga tcaaatcgtc ttcatcttca
     1801 aatgataaat caaaattatc aagtggacgg taagcaaaag ctagtacacg taatgcgcgt
     1861 gttgaaaaag cttcattttg ctcttggaat tttttcatca attcatccgt catcggaacg
     1921 acttcaccat ttagcaatac ttttttactg cgttggaaga cgatatctgg tccgccttta
     1981 acaaacaagt aatttttgtt atcaattgtg tgtccagtag acattaattt tcgatctgag
     2041 tcaaaaggaa gttctgcttg tcttgggtag ttgtttcgga tttcctgata aggctggttt
     2101 aatttttcac taaagtcgat aaacgctact tccgtagggt cgcccaattt agtaccgtct
     2161 tcactgatcg atgcatcatt cgctaaaaca ctgatttcga tcaagcgacg ttcgtcagct
     2221 gtccattttg aagggtcatt tgtgaaatcc ccagtatcgc cattacctaa aaagtagtca
     2281 acaactgtca ttttgttttg tgtcaatgta ccagtcttat ctgtacagat gatacttgtc
     2341 gaaccaagtg tttcaactgc tggaagcttc cgaataatgg catgacgttt tgccatttta
     2401 ttcgttccca aagataacac gattgttacg atagactgta atgcttctgg aatagcagct
     2461 acggctacgg ctacggcaaa catcactgcg ctgacgatat ctgcagtcat atctcctgta
     2521 ccttctccaa gataaatacg aaacagttgg atacctaaaa tcaataaaga caatgccaaa
     2581 atagccaaac ttagtttttt actgaattta tccagacctc gttgtaacgg agtgacttgc
     2641 tcagttgttg attccaaaag gccagcaact tgtccgatct ccgtattatt cccagttgaa
     2701 gtcactaaga atttaccgcg tccgtaagtt acgatcgttc cactgaatac catatttgta
     2761 cgatctccga ttggtaccgt acttgtgata tccgttactt ctttttctgc agaagtcgac
     2821 tctcctgtca gcataccttc atctactttt aatgatcccg catctagtaa acgtccatca
     2881 gcaggtacat aatcgcctgc gtctaacaac acgatatctc ctaccacaag ttcgtttgcc
     2941 ggaatcgttt tttccttacc atcacgaatc actcgagcat cgggcgctga aaggtttttc
     3001 aacgcttcta aagaaccttc tgcttttttt gtttggacaa cactgattat agaattcaac
     3061 atcaatactg caaaaataac taagaattcg atgtagtcac ccatgaccat ctgaatcagt
     3121 gcgacaatca gcaatacgat aaccattgca tctttgaaag tttctaagaa caatttccaa
     3181 gttggcttct tcggcttttc ttttagctgg ttcaagccat ctctttctaa tttttccttc
     3241 gcttgcgtgg tgctcaatcc atcggttgag ctctgcgttt gctgcatcaa cgagtcgatc
     3301 gtttctttat agaccgcttg ttgtgcttgt tccttgtttt ccaaatttca tcccccattt
     3361 tttgatcttg gccctagtcc tgtctgaaaa aaaagagacc taagacaaat acgcatttgt
     3421 cttaggtctc accatttcat acaataccag aaaaccattt ggttaactta ctgttgatat
     3481 tatcgcaaca aacgttgccg gttactcccc taaggagata ttcaactgta ctaatatcct
     3541 accttattaa cctacaaaaa gcaactactt tagcccgatt tacctacatt ttcatgaaag
     3601 gagtgaaaag tatattcacc ttggtaggct tctttcttct atcatgctat actaatgtta
     3661 ttgtatgtaa ggaggaacat ttttgtggta tctagtatta taaccgccct ttatctatta
     3721 aatgcactta ttgctttggt ggctattttg atcaaacccc gagatgtagc agccatttgg
     3781 gcatggctct tagtattgtt tgcccttcct ggggtgggct ttgtattata tttatttttc
     3841 ggacgtggat taacggataa gaaaaaattc tatctccgac aaagtgactt gaaagaatta
     3901 gaaaactttc agtcttttag aggagatacc attgaacatt acgatcctga catgggcgat
     3961 aaagacaaac agcaatttgt tgacttcttc tcttcattaa atcgtatgcc gctgacaaga
     4021 atgaattctg tcactcttct cacagacgga caagagaaat tggattcact gcttcaagat
     4081 ctaaaaaaag ccaaacattc gatccatatc gaatattacg catttgtgac agataatatc
     4141 ggccagcaag tcttacatgt tttagaagaa aaagccgcag aaggcgtgga agttcgaata
     4201 ttatatgatg catttggctc tcatggcaca aaagcaaaag atttcaatcg tctaatcaaa
     4261 aatggtggac atgtccatac atttgttacc tcacaaaggg cattacttcg tttccgattg
     4321 aattaccatg atcaccgaaa aatcgttgtg atcgatggaa agattagtta taccggtggt
     4381 ttcaatattg ccaatcaata tgtaaataca acaaaaaaat tcggctattg gcgcgatacg
     4441 catatacgga ttttcggtgc cgcttcttct ttgctccagc ttcgcttctt aacagactgg
     4501 aacgtctcgg tacctgaaga aaaaaaggtc ggctatcatt tgaattattt ctttaaaaaa
     4561 gcagatcgag atgaatctaa gcttgctgat acatccatcc agcttgtttc aagcggaccg
     4621 aataacgaaa gggaacaaat caagctttca tttatcaaat tgattacttc tgctaaaaaa
     4681 cgtgtttgga tacagacacc ttaccttgtt cctgatgaaa gtgtcattgc tgctttaaaa
     4741 atcgcaactg cctctggtgt agatgtgaaa atcatgattc ccaacaaacc ggatcatcct
     4801 tttatttatc gagcaacaca atattatgct cggcagctga tcaaggaaaa tgtacaaatc
     4861 cttgtctatg agaacggctt cctccatgca aaaacattga taatggatga tgaaatctgc
     4921 atggtaggtt cagcaaatca agatattcgt agctaccgat tgaattttga aacaagtgct
     4981 gtcatttacg atcctgagtt tttagaagaa cttgctactc agttcaaaga agatgagaca
     5041 cattgttcat ccatgacaac tgaaacagtc aaggaaatgt ctaactggct attattcaag
     5101 caacaaattt ctcgattatt ttctccaatc ctataatttt tttattcaag tagagaaaaa
     5161 tatactaaga ttaaagggac tgtgacaaaa atcttgtcac agtcccttct tccgaa
//
LOCUS       DACCQI010000150         5031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCQI010000150 DACCQI010000000
VERSION     DACCQI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.157"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVO76_002569"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IVO76_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7430501.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVO76_002570"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IVO76_002570"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7430502.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IVO76_002571"
     CDS             complement(2169..2906)
                     /locus_tag="IVO76_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7430503.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IVO76_002572"
     CDS             complement(2997..3524)
                     /locus_tag="IVO76_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7430504.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IVO76_002573"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IVO76_002573"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7430505.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IVO76_002574"
     CDS             complement(4401..4886)
                     /locus_tag="IVO76_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7430506.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1337 a         1123 c          862 g         1709 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt a
//
LOCUS       DACCQI010000151         4852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCQI010000151 DACCQI010000000
VERSION     DACCQI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.158"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            22..990
                     /locus_tag="IVO76_002575"
     CDS             22..990
                     /locus_tag="IVO76_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7430507.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            complement(1145..1882)
                     /locus_tag="IVO76_002576"
     CDS             complement(1145..1882)
                     /locus_tag="IVO76_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7430508.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1898..2731)
                     /locus_tag="IVO76_002577"
     CDS             complement(1898..2731)
                     /locus_tag="IVO76_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7430509.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            complement(2933..4261)
                     /locus_tag="IVO76_002578"
     CDS             complement(2933..4261)
                     /locus_tag="IVO76_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7430510.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT         1473 a          987 c          820 g         1572 t
ORIGIN      
        1 attcgtaagg aagtctgtaa aatgaaaaaa atacttgtac tacacaccgg cggaacgatc
       61 gccatgaaag aaaacatgct cacgggcggt gttagtcctg atgtggcaaa cccattatta
      121 gatgcagaaa tcacaatccc caagcatgta gaattattag ttgaagatat cttcaatctt
      181 ccctcaccac acatcatgcc ttctcacatg ttggtattaa aagaacgtat cttgtccgct
      241 tatgcatcag gtatatccgg agtggtgatc acacacggta cagatacatt agaagaaact
      301 gctttttttc ttgatacaac gattggcaac aaattaccaa ttgttttgac tggggcaatg
      361 cgttcaagta atgaacttgg aagtgacggc ctttataatt ttgaaagtgc gattcgtgtc
      421 gcaagttgtg aagaagcgct agataagggg gttttggtgg tcatgaacga tgagatccat
      481 tctgcacgct acgtgacaaa gacccatacc acaaatgttg ctactttccg aacacctaca
      541 ttgggtccca tcggcttagt aactaagaat cgaattttat tcttgcagga acttttagaa
      601 acaaagcgac tggacatttc cgctgtagat gggactatcc cgatcgttaa agcatatgct
      661 gggatgcaag gggacttatt ggaagccatc gcccatacga aagtagacgg tttggtaatc
      721 gaagctttag gtgcggggaa tctccctcca caagccttag ctgctttgga aaagcttctt
      781 gtccaaaaaa tccctgtagt acttgtttct cgttgtttca atggaatcgc agagcctgtt
      841 tatgattatg aaggcggggg aaaagaacta gaaaaaatgg ggattatttt ctgtaacagt
      901 attaatagtc aaaaagcacg tttaaaacta ttgattgctg taaactatgg tttgtcgggt
      961 gaagaattga tttcgtttat ccaaaactaa acatcattat ctaatagcta aacttaaaaa
     1021 gagaggctgt gacaaaaata gcttcttcga aaactaagtc gaaaatttac aaaaatttga
     1081 gaaacaattt tcgtaaattt cttcttattt cttgaagttg cccccttctg tcacaacctc
     1141 ttttttattt atttttccat ataatatttg cttcacaata cagctgttct tgcattcgga
     1201 tttcatgttt tgttttgaca ccattttcga tagggatttg ttcaaaatgg ctttcgatct
     1261 cattcccgta ctctacttct ttatcaaatc gaataattac tttttctgga acatgcgtag
     1321 tcaaaaagtc atatcctaat acgtcaatga tccaattaaa atacatcgca ttattaacat
     1381 gctgattact atcgatatca taaaaacgaa cacgatatgg caacatgaag cccgaatcaa
     1441 tcttttcaat tttttcctga cgcttgattt tcttgatctt ctcactctca aaaggagcaa
     1501 tgatctcttc attaacactg ctcatttttc gattcacaaa atccatcaat acgaaaacag
     1561 attctatttt gactaattct ttcccatctt catcatggat ccagaagttc cgataacaga
     1621 aaaacttatt gtactccatg gcttgagtcg ttactgagat tacttcaccg actctaggga
     1681 gacgattaat ctccatggaa taattcgtga tgacccaagt aagtccaaag ctattgacga
     1741 attcactgcc tcttcccaac tggtcgcttt gatcttcaga cgttttgata actactccta
     1801 acatcgaagg aaatgtcatc gtttggttga tgtcacactc ataatatgaa acttcatgct
     1861 ttttggtata tttttttgcc aaaaacacgc cctcctctta aaaattttct agtaattgat
     1921 ctacttggga accgtaactt tctccaaaca tattcaaatg tgccaaaaga taatataact
     1981 ggtaaatcgg cagtcggtct ttccatccct tttctaatgg aaatatagtt tgataagcat
     2041 ctaaaaattc cggtcggaaa ccaccgaata actggctcat tgcaatatct tgttcccgat
     2101 tcccataaga aacagctggg tcgacaaaaa ctggatgccc ttgctggtca aagaacacat
     2161 taccactcca caggtctccg tgcaagaggc gtggagtgat cttttttggt tcaaccgatt
     2221 tcaatacacg ttctttaaat cgcagatact tttcttgacg ctgtacattc catcgattcg
     2281 tttcttctgc caaagaaatc tgggattcaa gccgatcttt aaagaaaaaa gtccaccaat
     2341 cttcttcaaa agaatttttc tgaacaagtg tacccaaata gttatcttta cgaaaaccaa
     2401 attgtggcgc tgtttgctga tggagattag ctaaagctgc agcaagatct cgttgatccc
     2461 cttttcccgg ctcgatccat tccattaaca aataagcgcc ttcacttgtt tcacctagca
     2521 tatacgtatc cggtacacgt acgaatgctg acagttcttt taatccgtct acttctgctt
     2581 caaaaaatcc tttttttaca ttcggatgga tttttaaaaa ataagcacga tgttcggttt
     2641 ttatacgata ggtctgatta acatcgccac caacaactgg aatcacttta ccatttaatt
     2701 tcagatcaga taaaacagtt tggatatcca tgactttccc ccctactccc aattttattc
     2761 tacttcgtca tcatattttt aatgataaaa ggattatttc caatccttat cctttattta
     2821 gaattatagc acagggacca taaaaaacag ggaatggact ttaaagataa aaagagctgg
     2881 cttaagccag ctctttttat ctttactaca tactcttcat tgaaatactc gtctatgcta
     2941 aagcacccat tggatcccac ggagatagaa caattggttc ttcattgaac gcatttaatt
     3001 gctctttcgt caataaatct tttcttacaa cgatttggta cgtatattcg tccatccagg
     3061 catcactcat aacaaagaag ccattctttc caactttttc gccccagcta ttttcgactt
     3121 tccattttgt tgattgcccg tctactaagt caacaccggt caataccatc gcatgggtca
     3181 tcagactttc tccaaaatct aaacgttcag ctttagtcat tgtaaaatcc gtatcaaata
     3241 gatcatccat tgaataagca tctaaggcca tgatgccact agttctagta gaagattgac
     3301 ctacatcaca accaaaccaa acagattctc cttcttcaag ctgagcaatc gctaattttt
     3361 tgaaagtttc catatcaaca ttcagatatt tgacttcttt cccgccaacg acatttccta
     3421 gcatctctac tgtataagaa cgattgaatg gtttgtcttt tgttggtgca ttgatcacgc
     3481 taacataatc atctaatttt acaccgatgt atttatcata aaaaatttgt ggtgtcagtc
     3541 ctcggtcaag atgatagttc ttttcttcat cgcggtactc aaaatcgaat tcttttggag
     3601 gtgttcctaa tgagatagct aagaagttat aaatgctttc tagcatttct tctttctttt
     3661 cttcaatagc gtctaatgtt tctccttgag caaccatttt ccgtaacact actgcatctt
     3721 tacgcagtaa tttgttcaag tagttattca aatctcgaga atttgaacta ttcgcacttt
     3781 ccggcatcac cgatttaggc acaacgccat atttttggaa taatgaaacg atcatatccc
     3841 attggccgcc atcttgttga ggagtagcga gtaagaaagc tactttccgg ctatcgattg
     3901 cctcttttcc tgttgcaatg atgttttcat agaaataatt tgccttttca tatttatccc
     3961 agaaaaaagt gtaattttgt gataattcaa actcttttaa atggaattga ttcagcatct
     4021 tatgacggaa tgtgttcagt gcagcaaaca tccagcaacg ccccgattgc atttgattag
     4081 ccactttccc cgtagccaaa tctatagaaa ataccggtgt attctccaca tgtgctgaaa
     4141 cattttcagc agaagcagta attccgttct tcacaactgc ccgctgcaat gcctgctgtt
     4201 tgatatttgc ttgaaactgt ttgttaaatt ttgttgtcat atcaggcgta atagctttca
     4261 tccaatcatc cttccaaatc caagttatac ttcgtatttt actcttaaga atttagaaat
     4321 acaatctctt ctatgatgaa attttcattt gaatgagaat atacttacaa cttcatgaag
     4381 aaaaatgtca tacggtatac aatagttttc agataaaaag gaatatttct atgagataac
     4441 gaaaatggcc tgtataataa agaagaggcc tagaagaaca tcatacaata agatagcttt
     4501 caaaatactc attgaaagaa ggaaatacta taacgtaaga aaggagatga ataaaatgaa
     4561 aaacctgatt gctggcggct ttttgtttgt gggtggtggc atacttatga atacagataa
     4621 ctttgggaat gtcatccctt ttcttggtgc agcttgtgct ttgattggtg taggaatgct
     4681 gatttatttc gtatttagcg atgatgggaa gtatggttga ttatgagtag ccaactctac
     4741 tctttcgtaa agattacaac atacgtgaca aaaacagaaa gcttgattca tcataataat
     4801 aaaagaaaaa gaaacccaag tgattggatt tctttttctt tgcataagct tt
//
LOCUS       DACCQI010000152         4843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCQI010000152 DACCQI010000000
VERSION     DACCQI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.159"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..290)
                     /locus_tag="IVO76_002579"
     CDS             complement(<1..290)
                     /locus_tag="IVO76_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430511.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLL"
     gene            644..1492
                     /locus_tag="IVO76_002580"
     CDS             644..1492
                     /locus_tag="IVO76_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7430512.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1637..2578
                     /locus_tag="IVO76_002581"
     CDS             1637..2578
                     /locus_tag="IVO76_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP7430513.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2568..4739
                     /locus_tag="IVO76_002582"
                     /pseudo
     CDS             2568..4739
                     /locus_tag="IVO76_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         1518 a          945 c          878 g         1502 t
ORIGIN      
        1 aatagcagat ttttcagtaa acaaaacggg ggtgattgga tcagaaaaac aaacaagaga
       61 taaggttcgc tatatccttg atcataaact aactgaacgg ttttcttatg aagaatcagc
      121 tgaatgggac agtcgctttc cgccaaacgt ttcagatgcc gatccactgt tttaacatcg
      181 attttcagtt cttcagctaa atcagctttt cgccagatca aatctttcat tagattgatt
      241 agtgaaaata aattctgtgc attttttgat aatagttttt gccatctcat cgatcatttc
      301 tcacctgatt tctaaacata ttgtttgatc aaaatgggtt cactcctaaa aagataaaaa
      361 actccctttc tatttattta aatacgcaaa tcttcctcat tattttttat tttactcaat
      421 ttaatcgtat tctcatttca aaacgaatgt actttaaaaa aacataataa gatcactttg
      481 cttgtaggat gtattccctt ccattacaat gaaataaata gcgattttat gagaaggtga
      541 tgtgaaaatg gaagaaaaaa tagaagaatc caaacaaaaa atcgatcagt tgatataaga
      601 attagaaaaa gcggtcatcg gcaaacgttc aactatccaa ttgatgatcg tggccctatt
      661 agcaggtggt catgtcttat tcgaagatat tcccggtgta ggtaaaacgt tactggtcaa
      721 agcactagca aaagctgtac agggaacctt cagcagagtc caatgtactc ctgatctgct
      781 gcctagtgat attcttggtt tttccgtata caattcacaa tcaaaagagt ttgaatttcg
      841 tccgggaccg atctttacca cgatcttgtt agcagatgag atcaatcgta cgactcctcg
      901 tactcagtca gcacttttag aagcaatggc cgagaatcat gcaacgatcg acaacaatac
      961 ataccctcta gacaatcatt tttttgtctt agctacgcaa aatccaatcg agtacgaagg
     1021 aacatatcct ttgccggaag cacagctcga tcgtttttta ttccgactac aaatcggtta
     1081 tccttctttt gatgacgaac ttttgctact tttggataaa ttcgaaaaag tgttggataa
     1141 cttaaatgaa gtattgaaca gttatgaaat agaagaaata aaaacaaatg tggcgaatgt
     1201 ctttgtcagt cccgaagtgg catcttatgc cttgcagtta gtgtcagcaa caagaagtca
     1261 tgctgccatc cagcttggca tcagtccacg aggaagcctt tcctttattc aggcagcaaa
     1321 agcttatgct ttaatccatg ggagaaatta cgtcacgccc aaagatttgc aggatttagt
     1381 tccttacgtg ttttcccatc gcctgatttt ttacgatcgt tcattgaagg aagacgaaaa
     1441 gaaacaaatc attcatactc ttgttgcaca aattcctatt cctgtgaggt agattgaggt
     1501 agacaatgga aaaaatcaaa gattatttca ttaattttgt gttatttctg gtatatttca
     1561 tcattgtgct ttttgctctt gtattcaaca attctttagg ctggttcttg ttggcctttt
     1621 ttactcttta tgtatcatgg agcttgattt cattaagcag ctttctaaag aaactttcgg
     1681 ttacttgtac attgcagcca ctcgtttatc gcaaaaaaag tatgcaggtt attttttcca
     1741 tagcaaaaaa gagaactttt tctttacctc tccctcgttt acaaatcggc ttcccagcaa
     1801 tctttgcaga aaaaaacaag agattttgat tcttacaaat aaaccgcaag aagtccaatt
     1861 gctttggcag ccaaaagaaa gaggatgcca tgaaacatta actgtcattt ttacagccta
     1921 tgatagtcta ggacttttca gaaaaagtgc aaaaagagag atctcttttc ctgtgactgt
     1981 tctgcctgct ttttgtaaga ttcaagcaga aaaattacag agagttctgg aaaaaaaaca
     2041 gcagctgaac gcgtaccaaa gaggtcttga ttttcgggaa catcgcgctt atcgtccagg
     2101 tgattctttc aaccgaatcg attggaaatt gtctgctcat actcaagagt ggttataccg
     2161 tgaatacgat tatcaagaag aagaatgttc tcctcttttt tgcttttggg gttcgcccag
     2221 tccaaaattt gaacctctgc tagacttgta tttttcttta tggcatttga gaaaagaaaa
     2281 gcagccggaa cttctgatat taggcaaccg cgcctttcat ggcaaagacc ccggtcatac
     2341 ctattttgct tctttggaag ctggcgatga gaaaactttt ttcgcacact tgaaaaaata
     2401 tgcaaaaaaa caaatcgtcc tctttgttcc tgagaattct cctgaagtct cagaagctat
     2461 tgaaacattg tcaaaagatc gaacggttta tcttgtttat ttcgtagaaa aacaactcat
     2521 ggtccaagaa aaagacaaga tatgttcatt gccaggaggt gaatggattg acgattaaac
     2581 gggaaaaatg gctgcgtggc gtttactcct ttgtgatact aagtgtaggt gctccgatat
     2641 ttgtagtagc taatcaattg caaaacagct accttcttat cctaactatt ggtgtcctat
     2701 gccttattgt ctgctgtatt cctaaaaaat gggtacgttt gctgctttct ttgccagtaa
     2761 tgataggttg tttgttttta tattttcctt cgaagacatt ttccatatta tggatcttac
     2821 agtttttttc gtcagttaca gaggaattct tccatctatc ccaaggtaaa ctttattatt
     2881 tgccagaaaa gacagctttt tttctcataa tgttgctgat ctatttattt attacattga
     2941 ccgtggatta cgactattgg tacgcaccct ttctttcttt gatggcttac ttcatgatgc
     3001 ttaccgtttt tcggaaaaaa gatttactgt acgaaatgat tgcggtagta actgttttgt
     3061 tcgtgtatct ggcagcaagg caatttttct ccatccggtt gttgtcgaga aacactcgat
     3121 tccagtcatt gactacagtt ctgcttattt gtttcttaac ttttagcgct atcttgcctt
     3181 tatatcttcc gaaagtacac caatctctcg aggaaacgag tgaaccgatc cgagaatatc
     3241 ttaatgatga agggctttat gacacactcc atgagtatca attaaccgga agtgcaagaa
     3301 caggcttcag tgagaatgat gaacagcttg gtggaccttt atatgataat ggcgaagttg
     3361 tttttacagc gaaccaaaaa ggaaatggct attggaagat cgaaaataaa aatcattaca
     3421 ccggttctgg atgaatagaa gatcaggacg cacccatcca accaatccaa gaattccctg
     3481 ttgacatcac gagctaccaa accgttatag aagacaaaga aaacattaca ttgaccctaa
     3541 aaaaacaagc tttcttacct tatccttatg gaaatactac cttgactgat ttttcattag
     3601 ctagtggaca aacgctttac tatttgcctg aaagccagcg attcattcct gtcccgaatg
     3661 aatcgacacg ttctgcaact ttacaaacag aaaggttagt ttttacgaga gaagaattaa
     3721 aaagcagtca gttaaaagct gatgatttag aaaatcagca gacaaatctt caattgccaa
     3781 aaagtttgcc taaacgtatt gaggacttgg cggtcgaatt gaccaaaaat gaagcaactt
     3841 taattcgtaa agtcgaagct gtctaacagt atttaaaaac aagcggaaat ttccgctatt
     3901 ccaaaacgga taccgggcat actccaaaag aacacgatta tgtggattat ttcctctttg
     3961 attcaaaagt cggctattgt gacaactttt caacttctat ggtcgtcctt cttcgaacac
     4021 ttggtatccc agctcgctgg acaaaaggtt ttacaaatgg gctcagaaca ggcacagata
     4081 atcaagggaa tgaggaatac agcattctca atagtcatgc tcattcttgg ccagaggtat
     4141 actttcctgg atttggctgg cttccatttg aaccaacccc ttcatttgcc aatccaatgg
     4201 gttcacaacc agttgctgaa acaaccgaaa ccgtccagcc tgctatcgaa caaccaaatg
     4261 aaagcgaaaa tatggcagaa actatttcca gtgagagatc tgaggtcatt ccatcagaag
     4321 aaacaacact cacaccagct tataaaatcg tcttattcag ccttggagtg tgtttatccg
     4381 tcttgttgtt ctttctattg ttattcagaa aatctctgcg ctggacaagt tatcttatct
     4441 atcttcattt ttttgcttct actaatacga ttgcatatca aaaacttctt aaccctttgg
     4501 aaacattggt accgcgcaaa cccgcagaat ctttgcagga atatagcctg cgaatagaac
     4561 aagaattcaa gctagatctg cgtttcattc gattaactac tcaatatgaa gcagaaatat
     4621 atggacgaca agcagatccg gtaaaattcg ataaaaaaac ctatttagaa ataacgcata
     4681 taataagaaa aacggtatac aaaaaaattc tgacaaaagg gagccgcttc aaaaaataag
     4741 ataaaatttc tggagaatct acttctcgaa tgccgagaac cagctgattg agtatcgatt
     4801 actcgaaaaa aaggttgtga cacgtttttt gtcacaacct ttt
//
LOCUS       DACCQI010000153         4784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCQI010000153 DACCQI010000000
VERSION     DACCQI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.160"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            560..1327
                     /locus_tag="IVO76_002583"
     CDS             560..1327
                     /locus_tag="IVO76_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7430514.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            1353..1898
                     /locus_tag="IVO76_002584"
     CDS             1353..1898
                     /locus_tag="IVO76_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430515.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFDGQQIGLKKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            1899..2465
                     /locus_tag="IVO76_002585"
     CDS             1899..2465
                     /locus_tag="IVO76_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430516.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            2480..2728
                     /locus_tag="IVO76_002586"
     CDS             2480..2728
                     /locus_tag="IVO76_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430517.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            2748..2987
                     /locus_tag="IVO76_002587"
     CDS             2748..2987
                     /locus_tag="IVO76_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430518.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEQLLRKAQQKGYPDKMLKQIGYYT
                     PEERIEKESCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            3004..3588
                     /locus_tag="IVO76_002588"
     CDS             3004..3588
                     /locus_tag="IVO76_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430519.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEQELILERQSILCSEQDSKRLYGLYDLQLNGLKAQYV
                     ADSFDDMVGQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSIVES"
     gene            3695..3976
                     /locus_tag="IVO76_002589"
     CDS             3695..3976
                     /locus_tag="IVO76_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP7430520.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFNEA"
     gene            3966..4307
                     /locus_tag="IVO76_002590"
     CDS             3966..4307
                     /locus_tag="IVO76_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7430521.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWVDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIERLQRKV"
BASE COUNT         1808 a          580 c         1041 g         1355 t
ORIGIN      
        1 ccgtcctagc catcgcctag tccgattttg cttgatcggt tccatttctg tttatttagt
       61 gggtcaacac cgtaatctta aatagccatc gcctatttaa gattactagg tctgctttga
      121 aggtaaaaag tctttcattc ttcaagactt ttaggctttc aagctacgcc tgaaagcatt
      181 gaaattcatc gctctttatt tggttttgcc atttataatt ccttacgccc attatctttt
      241 tcgcctgtca taccttgtca gaagtgttta gagcttatct catctttgga ttaatacaag
      301 tggcgtaaat ttttttctaa acgcaagcgt tacgaaaata atttacttaa acacttgtat
      361 taatccggca gataagaacg cattctaatc actcccgaca aggcaagaca ggcgaaaaag
      421 ataaataaat gaacgaaagg aagaaacata aaatgcaaaa ccaaatagcg atgaagaaaa
      481 aaccacctcc ctctaaaaca atccaaacaa aaaagaattt tgaaaaacca aaaatcaaaa
      541 acagaaagaa gcgttcaaca tgagagatag aattttagaa gttaatcaag aaatggtaaa
      601 caagttaaca aatatcagtg ggaactatga aaagtacgat ggtactttac ttgataacta
      661 tatttttcac gatacacaaa atatcagaat tggcagaagt aaaccgagag cgttcattct
      721 catcaaagag aaatatttga atgaatggaa tagcacctat gaaatggtta tgactgataa
      781 ctttgaaaag gtattggatt tcagaaatgc tttgttgggt tctgatatgg gaaatagcag
      841 tcttgaatct attgaagttg aaaacaagac cgagaatgaa ggtgaaggat tgattaagaa
      901 tgtcttgaaa atcgaacaaa aatatatcag tgaagataga agtttttata gagtcacaag
      961 ctcggttggt ctagcaaact ttgctactaa cttaattgga aacaatgcta gtgaatcttt
     1021 gctagtgatg tgtctgaata caaagaatga ggtcattgct tatagcgaag ttttcaaagg
     1081 tggaattaat caatcagtag cgataccaag agagattttt caactagctt tgctaaacaa
     1141 ttcagcaaaa atcgcagtat cacataatca tccgagcgga tatgaatttc caagtgaaaa
     1201 tgacaaaagt tttactacga atttgaaaaa cgcaggaaaa acaattggta ttgaattgtt
     1261 agaccatatc atcgtaacaa gtaaaaacac aagctattac tcatttcaag aaaatggttc
     1321 tttataaaaa ttagagaaaa gaggaaaaca agatgggtga aagaacacaa gcgttaatta
     1381 cagtcaaata tggtaacagc aaaacgaaag caagcgtgtc aattcattat cagtggggat
     1441 atggtcgata catgttgatg gacgttttga atcaaggaat atatttaatc aataaagcat
     1501 atgacttacg agatgaatca agagaagaat tagcaaaagg aatctttgat agaagtacag
     1561 gagttatcaa ttttgtcacc tatgaatctg gagtgagtgc ttatgatgaa acagggtttg
     1621 gtgggcgcta tgaaccttat caagaaacga ttgaagatgt gatgaagttt tcagataaca
     1681 atgatggcta tgctcattta gagatggtgt tcgatgaaaa tggatttagt ctagatgata
     1741 aaaccagatt gcgattgttt gattttgatg gtcaacagat aggactaaaa aagttttgtg
     1801 aaagcggaaa agagtttgct acgaaagatt ttattaaggg atacagatta ttgctagaaa
     1861 attatggaat aaacctagaa gaagagagcg tgaaataagt gtttgaaaaa agaaaagacc
     1921 agttccctaa tttagaagcg aaaagtcaaa aagtgattga tggtgaaatg attgaattat
     1981 tccaaaaaag ttatggaacc atagaaacta ccgcaagttg taaatactac tttcaaggaa
     2041 agagaaacac gtttaaaggc tttgccgatt ctgatgaaga attggcaaag aaaatcattg
     2101 attggcttgt cagcagtgaa cacgtagcaa gagaagatac cgttagaaca acgattaatg
     2161 gggaagtcct aacaggtact agatgtgttt gttgtaatca tgaatttgta gaagatgaag
     2221 gttgctaccg aatcaatgaa tatggtgatt ttatttgcca gagttgccac gagatagaat
     2281 ttccaaccga aagaagttgg tttgaataca tgctacaaga ttatgagatg gaaaaagaaa
     2341 atggtgaagt agttttttgt accaaagagg aattgagtaa gagaagcgat gatgaaattg
     2401 aagattggtg tgtgtggtta gtaggggcag atgattgcgt tgcatattac acaacagcag
     2461 aataggagtg tgaatcagca tggaaggaat ggattttcta gatcatgaag atttggttga
     2521 ttttggttat acatggaaag gaatggtagg tattagcaga agtcttgcta atgcctttta
     2581 cgaaagaaat tatgcagtgt atgttcttta tgatgacgat acagaaagcc ttgttgacga
     2641 ggaatataaa ttggatttag agaatgtttt atatggaata gaaaaggaag atttagcaaa
     2701 atatattttt tcatggttgg gtcaataagt gaaaggaaga tagaaacatg acaaaattta
     2761 aaagtaaggg tatgcaatgg tgttcttatt attatgttgg gaatgatgaa cagcttttga
     2821 gaaaagccca acaaaaaggt tatccagata agatgttaaa acagattggc tattacacgc
     2881 cagaagagag gatagaaaaa gaatcttgtg aggtactttt tgatagtgaa cagctattag
     2941 ttttgttaac tcaaaataaa ttaatttgtc aatttgagaa taattaatat ggggtgaata
     3001 accatgaatg agaataagat ggtattaagt gaatacgcaa gattgagaac cattaattgg
     3061 ttgaatggta tgcaaagtag tggcgctttt gatggatttg atgaagagaa tatggacttt
     3121 gaaagtttat cgaatgagga tattagatat tattttatcg aatggtattt tacagaacca
     3181 gatgaagagg tggaagatga acaagaatta attttagaac gtcaaagtat tttatgcagt
     3241 gaacaagata gtaagaggtt gtatggttta tatgacttgc aactgaatgg attaaaagcg
     3301 cagtacgttg ctgatagttt cgatgatatg gtaggacagg gggcagatag ggttgtcact
     3361 tattcagaag atgatgaaga ggaatgggaa aactattcag atgatgagat atgctttaat
     3421 gatgggtata tgcctattga aatcaccgaa gaagaatatg ataaagtgtc cgatagtgat
     3481 gagattggta tgtgtacggt tgtaagtgga ttggatatag atgtccaaag agtagaggtt
     3541 gcataccaga agatgaaaga acgtataaaa agtatagtag aatcttgatt taattggatt
     3601 gtttgcaaag tatctaatta agagtataat ataatcataa atgtagttaa tggatacaat
     3661 aatgagtata ttatacttaa aagaggtgaa cgaaatgaag gtaaaaaatc caactgaaaa
     3721 aagcagaatc caagtcggaa tagacaagaa tttaaaagag aacgcagaaa tgatattgga
     3781 agagctaggt ttaaatccta caacagcaat cacaatcctt tataagcaag tggtcgctag
     3841 aggtgagttt ccagtagaga taaagctaag tgaagaggaa aagcaaggaa tcagattgca
     3901 acagctaact aaggatatgc cagttgatgt tctagataca gatgaaaagt tagaagagtg
     3961 gttcaatgaa gcctaatgag atagtaacgg tctatgttgc atttacagat aaaaatggtg
     4021 gtaaaagaag acctattttg gtggttagtg ataaagaaga tagagtagaa ttttttggaa
     4081 tcacaagtca atatgagaag aaatcagacc aaataaagag agtgtatttt cctattgcag
     4141 aatgggaaaa agcaggactt aaaaagcaat catgggttga tgtagggtct ttaaaggcta
     4201 ttcctaaaag ccgagagaac atatcattta aaaaggttgg tatgctatca gctagtgata
     4261 cgaaaaagct aaataggttt atcgagagat tacaaagaaa agtctaacaa ggcttttctt
     4321 tttttgatta tatgtatatt aagatatacg tttgtataaa tgtatttatg tatataaaaa
     4381 taaacgtaaa tatatacgtg attataaata ttaagaaaac attaagaatt gtttaatagt
     4441 aataattagt aatatacgaa ttataagatg taatttatat tgaatgtatg taaataaata
     4501 aaatggctga cggatattga atagcaataa ataacaaaaa gccagacgga tacagtgagt
     4561 gaagtagctt gctacgaacg aacagacaga atgaaacggc agtttcatta gagcaagtcg
     4621 cttgcgacgt agcgttaccg ctgatgtaac tggtaagtta catagagaga gtttgcttgc
     4681 aaacgaacgt tacctacttg aatagaaata aaaaaataac atattagtga attttgaaca
     4741 ggagccacaa ctcacccaac cgcttgcggt gggtgagtcc cacg
//
LOCUS       DACCQI010000154         4542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCQI010000154 DACCQI010000000
VERSION     DACCQI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.161"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..280
                     /locus_tag="IVO76_002591"
     CDS             <1..280
                     /locus_tag="IVO76_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7430522.1"
                     /translation="RQFHNEKDLAISISLEASELLELFQWKTPEEVKETQLERIKEEL
                     ADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVRKKYTEL"
     gene            348..1061
                     /locus_tag="IVO76_002592"
     CDS             348..1061
                     /locus_tag="IVO76_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7430523.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            1364..2215
                     /locus_tag="IVO76_002593"
     CDS             1364..2215
                     /locus_tag="IVO76_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7430524.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            2336..4201
                     /locus_tag="IVO76_002594"
     CDS             2336..4201
                     /locus_tag="IVO76_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7430525.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            4215..4542
                     /locus_tag="IVO76_002595"
                     /pseudo
     CDS             4215..4542
                     /locus_tag="IVO76_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877189.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
BASE COUNT         1490 a          736 c         1034 g         1282 t
ORIGIN      
        1 gcgacaattt cataacgaaa aagatttggc tatctctatt tctttggaag catctgaatt
       61 gttagaactt tttcaatgga aaacgccgga agaagtaaaa gaaacacaat tagaacgaat
      121 caaagaagaa ctggcagatg tattgatcta ttcatacatg atggcagaca accttcacct
      181 ggatctagat gagatcattg aagaaaaatt gatcaaaaat aatttgaaat atcctgtaga
      241 aaaaagcgca ggtgtacgaa aaaaatacac agagttgtag aaatgtgtca ttcggctgtt
      301 ggaaagagca aaagaaaaac agtaaaatga gtctggaggg agagaaaatg aaaaaaaaag
      361 gaaaagcaaa aagatggcta gtcaatatcc tcttgttcct cttgttatta gtaggactag
      421 ctttgatttt caatgaacag atcaaggatt attttgtcag agagacagga gataaatatg
      481 ctatagcgaa tgtaacaaaa gaagatctga aaaagaataa cgataaagat gtcagttttg
      541 actttgatgc ggtagaacca atgacgacag aaggggtcat gaggtcgcaa atgagaggaa
      601 cagatctacc agtgattgct agtatcgcag tcccttcagt ttcaatcaat ttaccggttt
      661 tcaaaggctt ggataatacg tcactacttt acggagcagg aacattatct ccagatcaag
      721 aaatgggaaa aggaaattat gccttagcaa gccatcgtgc aacgaaccct gaattgctgt
      781 ttacaccgct agaaaattta gagatgggtg ccaagatcta tttgacagat ttagaaaatg
      841 tgtatacata caaaacattt tttaaagaaa aagttgctcc tacagatacc caattactga
      901 atgaagtcga gggaaaagaa attgtcacac taattacttg tggcgacatg gacgccgtca
      961 cgcgactagt tgtacaagga gaactggaaa gtgttacatc gataaaagat gcgacagatg
     1021 atatgagatc tgctttcaat ctagagacaa aaacatttta ggatactggg acataagtcg
     1081 ttacagatta aaaacagcca aaggatatga atcgataagg aaaaaccaat ttgctgaaaa
     1141 aaagagaaac gcaaagaggt gcatctctga tttggttgtt ttctagatag acttgtgttc
     1201 cagttttttt atatgaaaag gcttgcataa ataaaaattc gatggtatga tgattttgaa
     1261 taagaaatgg attgttactg tacggcaggc aaaaccagaa ttcagctaag aaacagtagg
     1321 cgaattctag gtttttttat ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat
     1381 tttgaataac aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg
     1441 aaaaggacta gcttttcaaa aaaagattgg ggacgagatt tcgccagaag cggtccaaaa
     1501 agaatttgtt ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat
     1561 tccggcagag gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa
     1621 agttgaattt tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac
     1681 acgagcaaaa gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta
     1741 tccgaaagaa tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt
     1801 ggaactttct gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg
     1861 aaacgaagac atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat
     1921 catcagtaaa ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat
     1981 tactcatctg cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga
     2041 tgaatttctc tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg
     2101 gatcaatgag tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta
     2161 tttaacgatt catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt
     2221 gttactgtat ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg
     2281 tttttttcaa tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga
     2341 taatcaagca gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag
     2401 tttagtacat tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac
     2461 gcaaaagtta aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca
     2521 agtagtgatt ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct
     2581 tgcagtttcg gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat
     2641 tatctctagt atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg
     2701 attcttatca ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat
     2761 attgtttgct gcagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc
     2821 agctaaaaag ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta
     2881 tccagctatc acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt
     2941 gattctttca ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt
     3001 tcaaagtaaa ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt
     3061 agttccgctt gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat
     3121 cgggacggtt gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc
     3181 aatcgtagca ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca
     3241 ttggggattc gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat
     3301 gccaatgctt cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg
     3361 aacaaaagat acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg
     3421 aatcactgaa ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc
     3481 ttgtatttct ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc
     3541 cagcagtttg gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga
     3601 gtcaaatgtg acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg
     3661 tacattgatt ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc
     3721 gaatgcagga gaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa
     3781 agtactgcca ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg
     3841 tatcgctatt gatccagaag taggagaact agttgcacct gcagacggag agatcacgac
     3901 tatttttcca actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat
     3961 tcatatcggg atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca
     4021 aggggatctc gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc
     4081 agctggctat agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat
     4141 acttgaatta gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata
     4201 aggaggaaat caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg
     4261 ctgccaaatc aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat
     4321 gctatgcctg gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg
     4381 attgatcaag aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc
     4441 aaagaagatc ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc
     4501 tggacacgaa tttttccaaa tggagacgaa ggtactccta ac
//
LOCUS       DACCQI010000155         4516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCQI010000155 DACCQI010000000
VERSION     DACCQI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.162"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..95)
                     /locus_tag="IVO76_002596"
     CDS             complement(<1..95)
                     /locus_tag="IVO76_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7430526.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIY"
     gene            complement(140..904)
                     /locus_tag="IVO76_002597"
     CDS             complement(140..904)
                     /locus_tag="IVO76_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7430527.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(932..1630)
                     /locus_tag="IVO76_002598"
     CDS             complement(932..1630)
                     /locus_tag="IVO76_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7430528.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAR
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILDEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLNKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(1642..2421)
                     /locus_tag="IVO76_002599"
     CDS             complement(1642..2421)
                     /locus_tag="IVO76_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7430529.1"
                     /translation="MEETINLEEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDALGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(2437..3381)
                     /locus_tag="IVO76_002600"
     CDS             complement(2437..3381)
                     /locus_tag="IVO76_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7430530.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVAIVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(3427..4206)
                     /locus_tag="IVO76_002601"
     CDS             complement(3427..4206)
                     /locus_tag="IVO76_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430531.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSYVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1176 a          933 c          801 g         1606 t
ORIGIN      
        1 aaatagatat atcgtttttc aactttttct ttatccaacc aaacttgatt aggatgtact
       61 tcactaatac gagataataa atcttcactt tccatacttt ccctctctcc tcatttcctt
      121 tttatctacg aataaagaac taaaataaac caaacttctt cttttttacc tcttcataga
      181 ctggataact gatttcatcc ccgttgatca gatcctttgc gacttgtttc atctgtacta
      241 ctttttcctt accaaagtcc ttagcaatct gctcatagca ctcttttaag taaaaggtac
      301 gcttagagac cccatgggca tcagaagcag ccatctgcac gagattatgt ttgaccattg
      361 ttttggctgt tttctgaatg tctttgccaa atacaccaac ataacttggc gcagtaattt
      421 gggccaaaca tcccatatct aggaacggaa gcaaatgatt tgggtcttct cgaaaatgcg
      481 cgttgcgttc tggatggacg atcactggtg tctttcctag ttctaacaac tcaaaaaaaa
      541 gccattctgc atataacggc acttccagtg tcggaaattc gatcagaaga tatgtatcat
      601 ctaaatctgt aaataaaatt tcatctcggt ggatgtcttc gatgagcgtt ccggtaatac
      661 gtacttcttg tccttccaat aacgtcaaag gcaactgtct tttctctaat tcggcttgta
      721 acgaagcgac tagagaaatg acttgtgatt tttcattgct gtattttcca ttattgtggt
      781 gtggtgtgca taaaatatgc gtgatgcctt gctgaatcgc tttttcagcc attgcaatac
      841 ttgcttctaa gttttctgcc ccgtcatcga ttcctggtag gatatggcaa tgtagatcga
      901 tcatttctct tgtctcctct cttcttattc cttaatttcc gtagtaataa ccagcatctt
      961 tgctatgttc tgctccgttg tatacaacac ctaaaactcg tgcttgtacc atctctagca
     1021 gttcttttgc tttattcaat gattctttac gtgatgtatt ttcacgtacg accaataaag
     1081 tgccatccgt ttttgaagac atgatctgtg catctgttac ggctactact ggtggcatat
     1141 cgaatatcac cacgtcaaat aactgtcttg cttcgtctag gatctgatcc attcttggcg
     1201 aacttaacaa ttctgatggg ttgggtggct taggtccact aggcaaaatc gacaagttct
     1261 cgactggcgt tcgttggatt acatctgcta cacttcctga tgaactaagt gctgtactta
     1321 atcccctggc attgttcagc tggaatgtct tatagacgac gggtttacgt aaatctgcat
     1381 cgactagtag aactcgttga ccggatttag caaaaacgac tgcaatgttt gctgcagtgg
     1441 tggattttcc ttctccagga cctgatgaag tcacgacaat cgttttgatt tgctgaccag
     1501 ctgcggaggc aaactggata ttcgtccgga tcgtacgata ttgttcggcg attggtgaag
     1561 atgggtttgt taatgtgatc aaactgactg catgagtttg tgctgctttt tgtttctgtg
     1621 ttcgtgccat ttttcttcct cctagactct tgagcggctt ctgcgtgatg gggctgtttt
     1681 atcgtttgta gcttttggtc gtgcaggagc tgttcctttt ttacggatac cagcgttcaa
     1741 ttctttcgct gacatttctg gaactgttcc taagattggg aatcctaatg catccgtcac
     1801 atatttttca tctttcactg tccgatcaag taattcgaat aagaatgcca gacctacgcc
     1861 taccatcatt cctaatacta agccaatcac tagattcaat ttattatttg gcgaaacagg
     1921 tgtcgtattg gcaactgcac ctgaaataat cgagatttta tcgacattca tcacgtcttt
     1981 tgcgttctct tggaaaactt gtgaagttgt gttagcgatc tgttgagcag tcactgcatt
     2041 cgtgcttatt gcctggatcg agaacatttg tgaattttgt gactgattga cggagatcgc
     2101 atctttgatt tgtccagcag tcattttcac attgtcttct gtttctaaac gatctttgac
     2161 ttcatttagt actaaatcac tgacgatcat atctttatac gtattgatca tttgcaggtt
     2221 cgtattgaca tcattgacat ttgctgtgtc tgattgtggc agtgtcacga tcaattgtgc
     2281 ttgtgaactg tattttggtg tcaagatgaa aaatgttacg attcctgaga tggccaagcc
     2341 agcaaacatt gagatgatga ttgttaccaa gtgtttgcgt aatgtttcaa aaatttcttc
     2401 caagttgatt gtctcttcca ttttttctcc actttcctat ttattctcta gattcatttg
     2461 atcttttaat tcttttttta cacgatcaag ttcttgatca ctgacttttt gataggagat
     2521 attatcttgc ataaagcctt ctccttgcag ctgatcttgc ttgatcgtac caaatgcctc
     2581 ccgataatct aaagcgattt ttttcatttc atcaaagctc atgtctgttt tcatattcga
     2641 ctctagagca ttcaaaacgg attgatagtt gctgacaccg tttaaactca atactttttt
     2701 tgctacacct tctacgatct ttctttgacg ctcttgtcta ccatagtcac catttggatc
     2761 ttcataacgc atacgagaat aagataatgc ctgatccccg ttcaatgtga ttttaccgat
     2821 tgtaaagtca tagccatctt gtgagaaagt gaggtcatta ttgacttcga taccaccaac
     2881 tgcatcaaca agttccttta gccctttcat attgatagag acatagtgat cgattgggat
     2941 gtctacatat ttttgtacgg tatccatcgc catggcagct ccgccgaatg cgtaagcgtg
     3001 gttgatcttg tcttttgtgt cacgacctac gatgtcgaca tacgtatcac gaggaatgct
     3061 gacgatcgtt gtctgtttgt cttcaggact gactatggca accatcatcg tatctgatct
     3121 tccttgatcg acccgaccta aatctccagt atcaatcccc atcaatagaa cagagaaaga
     3181 ttcttgtttg tccagatcaa ctggtttcgt tcgttctcct tgggttcgtt ccactgattc
     3241 gtaagttttc tcaatagaat gtgagagatc cagatataat tttgctccta ctcctacaat
     3301 tgctaatact aatacgataa gaactgctaa aataatcaga aatatttttt tccctcttga
     3361 ttttcttctt cgtcttgcca tagtttcctc cgtttgctaa ccttgcttga atcaacgttt
     3421 taactgtcaa taacttatat tgctatagcc catttgctga gcaacagttt gtgcttttgt
     3481 tgcgtatgct gcgcttattt tttcattccg gatctgctct atttgactga gcgccgtcaa
     3541 atattgatcg taagtagcgg tggcagtcac ttgaccatcc ttcaacatcg tatcgaataa
     3601 ctgatctagt tgcgtgactt gcgcgatctg agtatcagcg aatcctaaat attgcataac
     3661 aaccgttttc caaggactat ctttaaaaaa gctcatattt tcacgtacac gagcgacatc
     3721 tgcacttgtc accttttctt tgatgatgac atcgtctaca gcttgctgcc agtttgatac
     3781 atttttagta aacaaatcgt tgacagctgt ctggatcttc agttttgatt ctgcactatc
     3841 taactgtgtc acgattgctt tcttctcatc ttggattgca acagcttctt taggcaattc
     3901 tgattctttg atgtcgtaat catctgctga aagtttgaca taggatagct tatttgacaa
     3961 acttgtgatt gtttcttctg ttatttcagg gtttagaaaa attttttctt ttgtactata
     4021 cagcttgtct acttcttttg ataaagaagt caaggtgtct ttttgattgg tgatggcaga
     4081 ttctgcataa ttgatccgtt gctgatgttg ccgctgatta ttgatatggt aaccaaacaa
     4141 acagatcgca atagcacctg cacctaccgc cacagcagtc aatgacttga cgaatggctt
     4201 ttccatagat tcactcctaa gtattgatga tagataaatt aagatataat tcacttacat
     4261 aaaaatgaca gctttttatt ttaaaaaaca taattaaact aatatattca cagaaatatt
     4321 tgtaaacaag ttttccctat ataatcatac cacaaactgt tttcttgact agggcaattt
     4381 atgatttaaa agtaaataaa acattaataa gataacaatt aatcgatttc atttttattt
     4441 ccttaatttt cataggtgtt tttaaaagtt acacaaaaaa taaaataaca aaaaaattaa
     4501 cattgaatta ataata
//
LOCUS       DACCQI010000156         4496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCQI010000156 DACCQI010000000
VERSION     DACCQI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.163"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            191..421
                     /locus_tag="IVO76_002602"
     CDS             191..421
                     /locus_tag="IVO76_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7430532.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            518..1819
                     /gene="murA"
                     /locus_tag="IVO76_002603"
     CDS             518..1819
                     /gene="murA"
                     /locus_tag="IVO76_002603"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7430533.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1832..2008
                     /locus_tag="IVO76_002604"
     CDS             1832..2008
                     /locus_tag="IVO76_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7430534.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2143..2439
                     /gene="yidD"
                     /locus_tag="IVO76_002605"
     CDS             2143..2439
                     /gene="yidD"
                     /locus_tag="IVO76_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7430535.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2624..3091
                     /locus_tag="IVO76_002606"
     CDS             2624..3091
                     /locus_tag="IVO76_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7430536.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3176..4060
                     /locus_tag="IVO76_002607"
     CDS             3176..4060
                     /locus_tag="IVO76_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7430537.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4177..4410
                     /locus_tag="IVO76_002608"
     CDS             4177..4410
                     /locus_tag="IVO76_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7430538.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
BASE COUNT         1536 a          683 c          978 g         1299 t
ORIGIN      
        1 ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt tgcatatttt
       61 tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat acttgttttt
      121 ccttttatac attttatggt attatcattt tattagttat tataaaacaa tcgttagaaa
      181 ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga gtcatttggc
      241 gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt ttttcagacc
      301 aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt tagggtacac
      361 tgcaagtaat tttttcttag aagttatcgc tttatgccga aatcttttcg ttagcctgta
      421 aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa gattttgagt
      481 aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca tcgtaagagg
      541 tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg ttttgcctat
      601 tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg ttccgattct
      661 ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg tagattttga
      721 tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg aagcgcctta
      781 tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac ttgccagaaa
      841 tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac caatcgattt
      901 gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg gctatatcga
      961 agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta gtgtaggtgc
     1021 tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca ttgaaaacgt
     1081 agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg gcgctcaagt
     1141 ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac atgcagtcaa
     1201 ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg ctgcaatgac
     1261 acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat tgatttctaa
     1321 attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg tcatcggacc
     1381 aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc ctacagatat
     1441 gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtag tgactgaaac
     1501 agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg ctcatgtcaa
     1561 aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg cagaagtata
     1621 tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag caaatggaat
     1681 cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc atatcaaact
     1741 acagcaatta ggtgcagacg ttgaacgtgt agacatggat caaacttctg ctgaaaaaac
     1801 agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata tcttattaac
     1861 tttgataaag attttaatgg tgatcttatt gcttatcctt ctttttgtgg ccggaacgat
     1921 gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtatttcagc caagtttgtg
     1981 gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc tagtcgatat
     2041 gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt gacgttagtc
     2101 gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt
     2161 tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat
     2221 tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggcgc gctaaaaggg
     2281 acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat
     2341 tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga accagattat
     2401 aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaacaattt ggaaaatagc
     2461 ttcttatatt ttgcaaaatt gtgagttatt gccgaagtgt tggattttga acaccgtcaa
     2521 aaaaaaggtt gtgaaaaata cggctagcac tgagcgataa agaaaaagag gaaatttatt
     2581 gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg aagaactaaa
     2641 caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag aaaaagttca
     2701 agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa caataggtgt
     2761 actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag taaccccaac
     2821 gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga agctttcaca
     2881 actcgcttcc agtcaaactc agatcatcat acgaaccggc agtactagca gtgcggcgtt
     2941 acatctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact attttatcca
     3001 tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag tgattttagc
     3061 aattcactta cctaaaaata gctttaacta ataaaatttc aattcaagaa aatgctttca
     3121 aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata tttttatggg
     3181 aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg atggcaaaga
     3241 gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga aagcgcaatt
     3301 atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg gaatcagctc
     3361 tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga ttccttatat
     3421 tcatggattt aatattttcc gagatttgga aactctgttt caacttccag taacgattga
     3481 aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc gcgattatca
     3541 agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca cgaaaggaca
     3601 aatcaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct taaatggcga
     3661 taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt gtgaacgcaa
     3721 aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag aaactggaga
     3781 tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg gattattcag
     3841 tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt ctgcaaaaga
     3901 agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat tcgatctgca
     3961 tgatttcgaa ccgcagattt tattatgtga atacagaaac gatgctaatc ttgtaggtgc
     4021 agcagcaaac tatctcgctg tacgtcaagg ggagaattaa gagctttaga gaaggtgcag
     4081 cttgcataaa agaagtgaat gaaatgaata ggagattttt acctaaggaa tgttttattt
     4141 gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa taaagatatt
     4201 gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga agtcactcag
     4261 ttatttatag ggaaaaaaat gattggtgag atcttagcct atggaccaaa agaatatgaa
     4321 attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc gatagaaaca
     4381 gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt caataaaggg
     4441 ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa accttt
//
LOCUS       DACCQI010000157         4331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCQI010000157 DACCQI010000000
VERSION     DACCQI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.164"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..552)
                     /gene="sgrA"
                     /locus_tag="IVO76_002609"
     CDS             complement(<1..552)
                     /gene="sgrA"
                     /locus_tag="IVO76_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7430539.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSE"
     gene            complement(748..1587)
                     /locus_tag="IVO76_002610"
     CDS             complement(748..1587)
                     /locus_tag="IVO76_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7430540.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1772..2659
                     /locus_tag="IVO76_002611"
     CDS             1772..2659
                     /locus_tag="IVO76_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7430541.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3253..3948)
                     /locus_tag="IVO76_002612"
     CDS             complement(3253..3948)
                     /locus_tag="IVO76_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP7430542.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(3932..4330)
                     /locus_tag="IVO76_002613"
     CDS             complement(3932..4330)
                     /locus_tag="IVO76_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7430543.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1430 a          857 c          644 g         1400 t
ORIGIN      
        1 ttcgctgctc gtgttttctg tgctactttc actgctcgta ctctctgtac tgctttcacc
       61 gctcgtgttt tctgtactgc tttcactact tgtgctatcc gtactgcttg gcggtccaac
      121 aactgtccaa ctgcttggtt tcaaattctc cgtgatcgtc atctgtccat cataaaaaac
      181 aatcaaatca actttatctt tactcttgat ctcatcaatc gatagttctg cagaaatcat
      241 tattgtatac tcaataccag cggtccattg tacgtctttt aaataaactt ccccaataac
      301 tttttctgaa cttccattac catcccaacc atatgttaga acagcatgta catcactagc
      361 attcacgctt ttgtttggtt tgactggaat agtaatgact tgttgtgtca tgtcataaac
      421 aatccgatca gttttcacag taaatgaaag attctcattt gcccgttcat ttgcagaagt
      481 actgattcca atcattgctg caattatact cagcccaaga ataacaaata atacggtcgc
      541 tgttttcttc atttctatca ctctccatat aattaccttc tatttgataa aggaattata
      601 taaaatgaaa atgatgagaa cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg
      661 tattcgcttg aatttgttta ctttttcgtt taatcaatga cagacttaaa aaagcaagac
      721 cgatatcagc cttgctttct tcatctttta ttgataaaca gcagtgtatt tcttcatccg
      781 ctttgtttta tatgcataga gtaacaaacc aacaatcaag actaacaaga ataatgtagt
      841 tgatatagat gcttcagccc caaagtttcc cccagtaatt attgtatttt gcgattggat
      901 cagagtggag aaaaaacctt gttcttgcgt tccgctgact ggtataccca aaatagctcc
      961 ttgtgtccaa ttccaagtaa tatgagcacc aatagccgta aataaacttc ctgtactgta
     1021 gataagtgcg ctgaaaaagc aacctgctaa aaagatattg aaaactgcca gaaatgtcat
     1081 gttaggattc aatccgtgca aaagagcaaa taatgcagca gatccaatca ttgctattaa
     1141 cggaccaaat ctaagcatct gatgctgaaa aaagccacgg aataaaagct cttctgatgc
     1201 ccccactgca ataaataaaa taaaactcgt tatcagcggc acaatgtttg cgtttcctga
     1261 atctaaccga tacgcccccg tcaaatataa tggactagta ataagcagaa acactccaat
     1321 tccaacaacg agacctcccg ctaaataccc ccagtctttt cctcgaacag aaacaccata
     1381 atcttctatt ctttttcctt cgaccttact agcaagaaat aaagctagta aaaaaacagc
     1441 taaatgtccg accgtaccgg caattgctcc aatccattca ctatttccaa taaacatatc
     1501 cgcaagtgca ttattgacaa gaattcctaa aacaaaacca actaagacta ttaaaacaga
     1561 tccaatcaac cgaataatcc tgatcatctt tcatccctcc tttatattaa gtttaacaac
     1621 ccttttcttt cttgtctcgt gaaaactatg taaatgttct ccatcataga gagaatagat
     1681 ttttacgaac taagaatatc tacagcaacc ttacgaaaat gaaagactgt ctccctaatt
     1741 cttctgttag actgttttta ataaggaaag gatgggaaat tttttgaaaa aacaactact
     1801 aattatgtgc ctttcagcat gtgcaatttt actattctca ggttgtagca agtccaatga
     1861 aacgattgca acgtttaaag gtggaacact tacagttcag gatttttatg ataacccaaa
     1921 agttaaatca aataatcaaa cactattaaa aaaaatgatt gtcttcaaag catttgatga
     1981 tgtatacgga aaagagattt cttcaaagga agtaacaaaa gaatataatg aacaaatcaa
     2041 aaaactcggt ccaaactaca aggaacaatt aaaagcagtt ggccaaacag aagagacata
     2101 taagaaactc tttaaacaga tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt
     2161 aacagataaa gatctagaca ctgcttggaa agaattctat ccagaagtta gtacacagat
     2221 cattctattt tcgactaaag aagaagcaga taaagcaaaa aaagaagcaa atgaaggaga
     2281 aaatttctcc aagcttgttc aagcttatgg aaaaaataaa acattaaagg aaactgatgg
     2341 aaaaatgaat tttgattcaa ccaatccaga gattcctact gaagtaaaaa aagcagcatt
     2401 taaactaaaa aacggggaag tttctgatat cattcctgta acagatccaa cgacttatca
     2461 gcaaagctat tatctagtaa aaatggtaaa aaaacaggat aaaggttcta ataaagacaa
     2521 atacaaatct gagctagaaa aaattgctac cgaagcaaaa actcttgata cggaattcat
     2581 ggataaaaca atccgtaaag tgatgaaaaa agacaatgta actattaaag atccttatgt
     2641 aaaaaatata ttcaaataaa caaatttgac ccaattgggg atgtggcaaa atttgagaag
     2701 tgatttttgg aaattccttc ttatttttga ggcaaaccac ttttgccaca acttcatcgg
     2761 ggcctttttt tatttgtacc aaaacaaatc actggatttg ttacaatcat gaggatataa
     2821 tttaaaaaag ttttttattt ctatcacatg aatcaatgcg ccattaagta gattctttca
     2881 tgcttcccta ttttttcact tgccgtatcg cttcaaaaac aactatcata aactcaaact
     2941 cgactgaaaa gttattttaa aaaagactaa aaatatccct tctggacttg ttcttcttat
     3001 tctgttatac tagagaaaca cgaaagatat ttttatgcga cacttttcat ttgtttttat
     3061 gaaatttatt caaaactggc gtaacttcag agttacaagg atgtattaga ggttagggaa
     3121 tacatcgttt aaatgagaaa taagtaatag ttagtaatag tcacccaaac tttaaattaa
     3181 tgaatattga atcgtcagat agcctcctcg agttttcgag aaggctatcg tttattttat
     3241 ggagaaattg attcagaagt aatcagcgac cttttttagt tcaccaatcg tttctgcttg
     3301 gaaagtttgc acccaatctt cttggaagcc cctcactgct ttatcaatcg agggcatctc
     3361 taatacttga tctagtagct ctttacctaa cgttatcgca tgagctccag atcgaatggc
     3421 tgtagtgact tgttcacaat ttttgaaact agctgctaat atttttgttg gtctttgcgt
     3481 acgttcgatc tcagtccgca acgaatcaat cactttcata gcatcaatgc cttgattttg
     3541 catcctgtta taataaggtg caatataatc tgctccatta ttaatagcta aatacccttg
     3601 taattctgtg taaatagctg taccggtaat caaataacct tcttttttca actttttgat
     3661 cgcttgaagg cctgcttgat tcaccggcac cttgataaac gtatcttttc ccagttcttc
     3721 tatgataact cgagcatcct ctatcatttc ttcttccgtt cttcctattg tctgaatatg
     3781 gataggaatc tgtccgatta tttttttgat ttctttcata tgcgagaaga agtttgacgc
     3841 tccttctttt ttcagaattg taggatttgt tgttacgcct gttaaattca aaaactgttg
     3901 acaagaataa atactttttg tatttgctgt atcaagcatg atttccatat agctttcccc
     3961 cattctgatc ctcttgatcg attttaatga ttgtcccctt tcttaaatgc ggcatttcct
     4021 tatcttctac atacattgtt cctggaagtt ccggctcatg agatccatca aattttatcg
     4081 tgatatgtcc taattcattc aaattagttt ggacaacatc tcctactgct gtgattacat
     4141 acatttgttt atcaaaacag agcttcatat cacaagttat ttcttttctt acctgattca
     4201 attcaatctt ataacaataa tctgttaatg tttccggtgc ttcatctcca aataaaatca
     4261 ttattttttc tttcctgaat acttctgctt ctataccaac atctgttaca accgtttcaa
     4321 agatcgtcat c
//
LOCUS       DACCQI010000158         4113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCQI010000158 DACCQI010000000
VERSION     DACCQI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.167"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            158..313
                     /locus_tag="IVO76_002614"
                     /pseudo
     CDS             158..313
                     /locus_tag="IVO76_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            332..1609
                     /locus_tag="IVO76_002615"
     CDS             332..1609
                     /locus_tag="IVO76_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7430544.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1619..2275
                     /locus_tag="IVO76_002616"
     CDS             1619..2275
                     /locus_tag="IVO76_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7430545.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2275..3651
                     /locus_tag="IVO76_002617"
     CDS             2275..3651
                     /locus_tag="IVO76_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7430546.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3781..>4113
                     /locus_tag="IVO76_002618"
     CDS             3781..>4113
                     /locus_tag="IVO76_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7430547.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1628 a          453 c          726 g         1306 t
ORIGIN      
        1 cgcgaggctt accagcgaga tagagatcga cgatttgttg tttgaattct tttgaatagg
       61 ttcttcgttg acgtcttggc atgaaaaatc ctccagtgtg ttttttatta ttctacacac
      121 cttatttttt ctgtctagtc tagtgtagcc gattcaggat aaggataagt gtattaattg
      181 tggaaaatgt aaaaaagttt gccaaatgaa taaagattca gttgagaatt gcaatcatct
      241 ggagtgtatc agatgtggac gatgtaaaaa tgcttgtcca gtggatgcaa ttagttatgg
      301 agtaagaaaa tagagaggag tagttaggaa agtgataaaa aatgctatag catatattac
      361 aagaaagaaa aatagaactt taataatttt tatcatatta actatagttc tttcttgctt
      421 gtattcttgt ttaacaataa tgaaatcaag caatgaaata gaaaaggctt tatatgaaag
      481 ttctaattct tcaatatcaa taacaaaaaa agatggtaaa tattttaatg ttaatcaatt
      541 taaagatatt gaaaaattaa aagaaattga agaaaaaata attcaatatg atggattagc
      601 aaaactaaaa gatgctaaag tagttagcgg agaacaaaga ataaatagag aagatttatc
      661 tgacgaattt aagaatgttg tttcactcga agctacaaat aatactaaaa gaaatatttt
      721 atttagtagt ggagtattta caattaaaga aggtaaaaat atagaagaaa atgataagaa
      781 ttcaattatt gttcatgaag aatttgctaa acaaaacaat ctaaaattag gtgatgaagt
      841 tgatcttgaa ttactagata ttgaaaaaag tggaaaaata aaaagtcata aatttaaaat
      901 tatagggatc ttttctggta aaaaacagga aacatataca ggattatctt ctgattttag
      961 tgaaaatatg gtgtttgtag attattcaac aagccaagag atattaaata attcagaaaa
     1021 taatgaaatt gcaaataaaa ttttaatgta ttctggtagt gcagaatcta cagacttagc
     1081 cttaaacaaa ttgaaagagc ttaaaattga tgagtcaaag tattttgttg aaaaagattc
     1141 taatgcgttt gaagagtctt tagagtcagt gagtggaata aaatatataa taaaagtaat
     1201 gacttattct attatgttag gtgggatggt tgttctttca ttaatcttga ttctatggtt
     1261 aagggaaaga atttatgaaa taggtatatt cttatctatt ggaacatcta agatacaaat
     1321 tataatgcaa tttatattcg agttaatatt catatcgata ccaagtataa tatcttcctt
     1381 atttttagga aatgtattac taaaagtaat tgtagatgga ttcattaact cagaggattc
     1441 aatgatttcc ggtggaagtt taataaataa tagtagtttt atgtcaaata taacaacact
     1501 tggacaaagt tatttaatat taataagtat tattgtttta tcagttgtat ttgcttcttc
     1561 attaatgtta atcaagaagc ctaaagaaat cttatcaaaa ataagttagg agaaaataat
     1621 ggatatatta gaaataaaga atgtagcata tagttatgca aattctaaag aaaaagtttt
     1681 gtcaggagta aatcaaaaat ttgaacttgg gaagttttat gcgatagtag gaaagtcagg
     1741 aacaggaaaa tctacacttc tttccttact tgctggactt gataaacctc aaacaggaaa
     1801 aatattgttt aagaatgaag atatacaaaa taaaggatat agtaatcata gaaaaaataa
     1861 tatatcttta gtatttcaaa actataattt aatagattat ttatcaccaa ttgaaaatat
     1921 tagattagta aataaatcag cagatgaaag tatcttgttt gaactaggtt tagataaaaa
     1981 acaaataaaa agaaatgtta tgaaattatc tggtggacag caacaaagag tagctattgc
     2041 tagagcattg gtatcagatg ctccaataat actagctgat gagcctactg gtaatctaga
     2101 cagtgttact gctggagaaa taattaatat attaaagaca ttagctaaag atagaaataa
     2161 atgtgtaata gttgtaacac atagtaagga agtagcagat tctgcggata tcattttaga
     2221 actaagtggt aaaaagttga aaaaagtaaa taaaatgaat ttggaggttg aataatgata
     2281 aaaaatgcat ttgcttatgt aactagaaaa agtttaaaat cattaattat catattagtt
     2341 attttatcta tgtcaacttt aagtctcatt agtttatcta ttaaagatgc tacggacaga
     2401 gcttcaaaag aaacatttgc taatataaca aatagttttt ctatggagat aaatagacaa
     2461 gtaaatccag gaacacctag aggtggggga aatgtaaaag gtgaagatat taaaaagata
     2521 tctcaaacag atagtataga ctcctatgta aaaagaataa acagtgttgc agatttagtt
     2581 gattatgata ttattgaaac tcaagagact cttgcgaatc aatcacctga aagagcaaag
     2641 aattttaaaa gaacagttat gttaactggg gttaacgact caacaaaaga aacaaaattt
     2701 gtatcagaag catataaatt agtagaagga aagcatttag aaaatgaaga taaaaataaa
     2761 atcttaatgc ataaagattt agctgaaaaa aataacctta aagttggaga taagataaaa
     2821 ataaaatcta atttatttga tgctgataat gagaaaggtg cagatgaaac agtagaagta
     2881 gaaattaaag gtctattcga tgggcataat agcggtgtag taagtgcagc acaagaacta
     2941 tacgaaaata cattaatcac tgatattcat acagctgcta aagtttatgg taataccgaa
     3001 gatacagctg tataccaaga tgcaacattc tttgtaaaag gtgataagaa tcttgatagt
     3061 gtaataaaag atcttgggaa attagatata aattggagag aatataattt gattaaaagt
     3121 tcatcaaatt accctgcatt acaacaatct atatcaggta tttactcaat ttcaaataaa
     3181 ttattttttg gctcattaat atttgcagga gttgtagttt cattattatt attcttatgg
     3241 atgaatgcta gaaagaaaga aatagcagta ttactatcat taggtatatc aaaattagaa
     3301 atttttggtc aattcctaat tgagatggta tttatatcaa tccccgcatt cgttggttcc
     3361 tatttcttag ctcaatatac agctgacaag cttgggaata atatattgaa taaggttact
     3421 ggtgatatag ctaaacaaat agctaaacaa tctgcatcta gccaattagg tggtggggca
     3481 gaagctgaag gatttaacaa gacattatca agtttagata tcaatgtatt acctaaattc
     3541 atgatttatg tagttatatt tatgagttta gtacttcttg tatcattgat tatttcttca
     3601 ttcaatatat taagaaggaa tccaaaagaa ttattaattg acaataatta aaattttcgg
     3661 tgctttttag caccaaaaat gagagtgtaa tataaaccgt gtaagtaacc atcaatccta
     3721 ggcgggtggt tgcttcacgg tttttatttt tctaaaataa gatcaagagg tgattacacg
     3781 atggctagaa agaaaagaaa tcctgatgct gagaagttag ctgaatccat tttgaatact
     3841 tatcaacccg aatctgtaga ggatatgcaa gatgttttaa aagatgtgtt tggtccctta
     3901 tttgaaaaaa tgcttcaagg agaattgaat aaccatttag ggtatgacgc ccattctaaa
     3961 gagccaaaga aacatgacaa tcgacgaaac ggttatggca ataagacgct taaaacgagc
     4021 tttggtaaag taaacattga tgttcccaga gaccgggagg cttcttttga acctgagttg
     4081 attcctaata gaaaacgaga tgtctccgac atc
//
LOCUS       DACCQI010000159         4083 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.168, whole genome shotgun sequence.
ACCESSION   DACCQI010000159 DACCQI010000000
VERSION     DACCQI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.168"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IVO76_002619"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IVO76_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IVO76_002620"
     CDS             complement(545..739)
                     /locus_tag="IVO76_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430548.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2091..3131
                     /locus_tag="IVO76_002621"
     CDS             2091..3131
                     /locus_tag="IVO76_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7430549.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3955..>4083
                     /locus_tag="IVO76_002622"
     CDS             3955..>4083
                     /locus_tag="IVO76_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7430550.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKY"
BASE COUNT         1495 a          567 c          614 g         1407 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtgatat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg caagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgtttt gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tat
//
LOCUS       DACCQI010000160         4073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.169, whole genome shotgun sequence.
ACCESSION   DACCQI010000160 DACCQI010000000
VERSION     DACCQI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.169"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IVO76_002623"
     CDS             591..1280
                     /locus_tag="IVO76_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7430551.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IVO76_002624"
     CDS             1267..2475
                     /locus_tag="IVO76_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7430552.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IVO76_002625"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IVO76_002625"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7430553.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1320 a          703 c          812 g         1238 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gta
//
LOCUS       DACCQI010000161         4069 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCQI010000161 DACCQI010000000
VERSION     DACCQI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.170"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..131)
                     /locus_tag="IVO76_002626"
     CDS             complement(<1..131)
                     /locus_tag="IVO76_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7430554.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKL"
     gene            complement(284..610)
                     /locus_tag="IVO76_002627"
     CDS             complement(284..610)
                     /locus_tag="IVO76_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430555.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(622..822)
                     /locus_tag="IVO76_002628"
     CDS             complement(622..822)
                     /locus_tag="IVO76_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7430556.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            897..1547
                     /locus_tag="IVO76_002629"
     CDS             897..1547
                     /locus_tag="IVO76_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430557.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            1602..2768
                     /locus_tag="IVO76_002630"
     CDS             1602..2768
                     /locus_tag="IVO76_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7430558.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(2857..3249)
                     /gene="rpsI"
                     /locus_tag="IVO76_002631"
     CDS             complement(2857..3249)
                     /gene="rpsI"
                     /locus_tag="IVO76_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7430559.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(3265..3708)
                     /gene="rplM"
                     /locus_tag="IVO76_002632"
     CDS             complement(3265..3708)
                     /gene="rplM"
                     /locus_tag="IVO76_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7430560.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         1334 a          730 c          646 g         1359 t
ORIGIN      
        1 cttagtttca ttttcttata tttttcattt acgtttaaaa aaataggata ttgatagtat
       61 aattctgcat atatatcaga attagaatag ccttgctttt caactaagct ttcaacttct
      121 ttttcattca ttgtcacacc tgcttctgat tttatttgct ataatagatt caaataaagt
      181 tttctcagaa ctttattatt gagtgttccc ccgatctgca aactgacggg aacactcttt
      241 ttaatttttt ctacttcatc aaatttaaaa aacgaggtgt tttttaaatt tgatgactca
      301 atatccattt tttgcattct tcttttgaaa acaactccat tttttctaat aaaataactg
      361 gcaatcccat tcttttaaat ttcaaaaaag ttgtatatga tacattcaaa tatttagcca
      421 tctcttttcc ttttagaaat ggtctttcta atccggtatc aattcttgct tgatcaatag
      481 cctcttttgt catttcaaaa acataatttt tcaattgatt cgtttgttgt ttatctaaca
      541 ttactgtaat tcctgcatcc attatttttc tcctctcatt ttctatttct tcaatcagcc
      601 aactcgccaa gttgtattta tttagatttt caaatcatta agtaaccaat ttaccaactt
      661 ttgataagta gatgctctaa tactttcttt gctcccattt tcgattttga catatgtttt
      721 ccgatttata cctatcaatt ttgctgcatc gtttttagta agtataagat cggctctatg
      781 tcttcttaag cttctaatga attgatcatt aagagttatc attcagcgcc tcctttcaaa
      841 ttattcactt tgtggttttt atcttaataa gcacatttta aaccacaaaa cgagtaatat
      901 caattaaaat attactcaat ttgtggtttt catgctataa tagtttggaa tttactatag
      961 ggggcgttac tttgaataga ataaaatttt atattgattc cagtaaaaag accattaaac
     1021 aaattagtac agaaactggt attaactata caactcttac gaactattac cgtggtgagc
     1081 gaaatcctag aaatactgaa acatggaata tccttgctga ttatttccat gttcctgtaa
     1141 gttatttgat gggattagac gaaacaaatt ccatagaaca acctaatctt gatagaataa
     1201 aaactgctcg tttagaaaaa aaggacaaaa tagtcaagaa tattgccagt cttgactcag
     1261 attattttac tgaattatct aatctatact tttccatgac ttctttactt gaagtcaaac
     1321 ttcaagaaga tgactccgac tttctttttt taaaaagtat tctagaggac tcctttgaat
     1381 acacgattct atttgccaaa caaaagaagt ttattaataa agctttaaac ttaaatatcg
     1441 atgaaagaaa agagttagaa tctgagttat ttggcaactt aacccaagta caggttaaca
     1501 atctaaaaag agagataaca gctcgattaa tatcaaaact tcaataaaac taattattca
     1561 atcagccaac tcgccaagtt atcagattgg agagattagt tatgtcaata caaaagtaca
     1621 caacaaaaag cggtgcacga tatagtgtcc gcttgtatgt tcgtaaagat gaaaatggaa
     1681 agcaagaata ctatgtaaaa aaagggttta aaactgaaaa agaagccaag ttacacgaag
     1741 caagaaaaaa ggtcgagatt gaagataccg gctatcataa gccttctaat gacttgttta
     1801 taacagtgta tgaagaatgg ttgccttctt ataggaatcg tgtacaagaa acgacatatc
     1861 aacgtacaaa agaccttttc cgcttgcata tactaccaaa attcgggaat aagtccattt
     1921 ctaagattac tcctgttcac tgtcagaatg cagtcaatga atgggcttta aaattcaaga
     1981 atatcaaaca gcttaagtct tacagctctc aaatatttga gtatgccatt tttgcagagc
     2041 tattacaccg aaatcctatg gcaaatacaa atctaccaaa gcgggaaaag aaagaaaatg
     2101 aaaattactt ttcattagaa gaactaaaag ctttttggga tattctcaat aaagaagagc
     2161 ctttaaaaca tattttaatc ttccagcttt tgattactac tggtatacgt aaaggagagc
     2221 tagctgctct tcattggtct gacatttatt ttgaagaaca gctaatgtat gttagaaaga
     2281 gctatgcaac tattcgaaat gaagagaaga atgcaagaag aaaaactgtc aggattcaaa
     2341 aaaatacaaa aaatatatct tccgaacgaa tattaccaat tgatgcacaa actattgaaa
     2401 tgttaaaaat ctggaaaaaa gaacaagctc gagagctttt gcagtttggc ataaatacaa
     2461 atacaaaaaa tcaacttatc tttacctatg taaatgcaga cggtgacatt aatcaacctt
     2521 tgcatgcgga ctattcaaat aatatattga aacgtttaga gaagaaatat aaatttaagc
     2581 acgttactat tcatggattc cggcacactc acgctactct tctactagaa ggtggagcaa
     2641 gtatcaaaga aacacaagat cgattgggtc ataaaaatgc tgaaactacc cttaacactt
     2701 attctcatgt tactgaaaaa gctcaacgaa atgcgttaga aaaattcatt gcttacactg
     2761 gtttataata ttgtcattac ctttctcatt accagaacga aaaaaagaca ccttctcgca
     2821 atgagaaagt gtctgaaatc gttggtataa caacgattaa cgttttgaaa actgtgaagc
     2881 tttacgtgct ttcttaagac ctggtttttt acgttcgacc atacgtgcgt cacgagtaag
     2941 taagccagca cgtttaagag ctaaacggaa gtcaggatca acttctagca atgcacgagc
     3001 gataccatga cggatagctc ctgattgtcc agcgtatcct ccgccgttta cgttaacaaa
     3061 tacgtcatat gcgccttttg tttcagtaac agcgaaaggt tgattgatta cttcacgcaa
     3121 gtcagcatgt ggaatgtatt cttcaacatc ttttttattt acagtaattt tgccagttcc
     3181 tggtactaga cgtacgcgag caactgcgtt tttacgacgg cctgtgccga tatattgaac
     3241 ttgtgccaat gaaattccct cctattagat taagtttgtg atgtctaaaa cttctggttg
     3301 ttgtgctgca tgtggatgtt ctgctccacc gtatacgttc aattttgtaa attgtttgcg
     3361 gcctaaagtg tttttaggaa gcataccttt tacagaagtt tcgatcaaac gacgagaatt
     3421 tttagcacgc aattcgccag cagtgattga tttcaatcct cctgggtagt tgctgtgacg
     3481 gtaataaatt ttgtcagtcg cttttttacc tgttaatttc actttgtctg cattgatgac
     3541 gatgacgaaa tcgccagtat ccaaatgagg tgtgaaagtt ggtttatttt taccacgtag
     3601 tacagatgca acaactgttg aaagacgtcc caatggaaca tcagttgcgt ctactacata
     3661 ccatttacgt tctacttcgc ctggtttggc catatatgtt gtacgcacgt tattttcctc
     3721 caatttaagt tctgatgttt gacatacact tcgagtttcc ggggctcttt gtggggcaaa
     3781 caataccatt taacatatta cccgctaatc tagtcaatgt caacgttttt ctgcttgatt
     3841 gaaaaattta tatgaaactt tacttctttt ctttctttta tataaaaggg tcgtttcctg
     3901 tcaaaatgaa aaacaaaaag aggctgtgac aaaagtcttg tcacaacccc ttattttgaa
     3961 taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccata aaacataaga
     4021 agctgttttt taaaattgtt tcttgtgact cggagctgaa cggcaggct
//
LOCUS       DACCQI010000162         4044 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCQI010000162 DACCQI010000000
VERSION     DACCQI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.171"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(168..410)
                     /locus_tag="IVO76_002633"
     CDS             complement(168..410)
                     /locus_tag="IVO76_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7430561.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(442..999)
                     /locus_tag="IVO76_002634"
     CDS             complement(442..999)
                     /locus_tag="IVO76_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7430562.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(1012..1200)
                     /locus_tag="IVO76_002635"
     CDS             complement(1012..1200)
                     /locus_tag="IVO76_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7430563.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(1213..1779)
                     /gene="amaP"
                     /locus_tag="IVO76_002636"
     CDS             complement(1213..1779)
                     /gene="amaP"
                     /locus_tag="IVO76_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7430564.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3009..3482
                     /locus_tag="IVO76_002637"
     CDS             3009..3482
                     /locus_tag="IVO76_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7430565.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            3491..3718
                     /locus_tag="IVO76_002638"
     CDS             3491..3718
                     /locus_tag="IVO76_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430566.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         1222 a          727 c          610 g         1485 t
ORIGIN      
        1 ctttttatag aaaaccgttt attttcgtct ttttttattt ttaaagatat tttttcataa
       61 ggaataaaga atacttcata taaaatatat aaaaaagaac cgcagcgagt gtcgctacgg
      121 ttctcaaatt tgttcttcat ttcaacataa gtttttcttc aatttgctta taatttacgt
      181 gcaaagaatg aaacgactaa tactaaaatg attgcaccga tcaatgatgg aatgatagcc
      241 attcctgcaa ttgttggtcc ccaagttcct agtaaagctt gtccaattgc tgaacctact
      301 aaaccagcaa tgatgtttgc gatgatcccc attgatgatc ctttattcgt gattgctcct
      361 gcaattgctc caatgatacc gccgacgatt aatgcccaaa taaatcccat tttaaattcc
      421 tcctaactga cttcttactt cttattgtac acgtggttcg ttattttctt tgactttttg
      481 tgcacctttg cctacagcag attttgcttt atctgtttgg cgtgaagcaa attgtcctgt
      541 ggattttgct gcttctgtta ctttgtcttg tactgtttct tgatcttttt ggtattcttc
      601 ttgtgttttg atgtctgcaa cattcacatt cacttcgata acatctaaat gtgtcatatt
      661 gtttacttca gtactgatca attctttgat ttggttgtaa atatcttcga tgtctttacc
      721 gtattctacg atcacatcta gatccacagc tacttgtttc ttacctactt ctgtattgat
      781 acctgctgtt acgttatctg tgttgactaa tttttcagcc atatttgaga aaaatccgcc
      841 atctaatgtc aatagaccat ctacatcttt caatgcataa ccgatgattt tttggatgac
      901 tttgtcttca taagtcaatt caccattcat catgcggttt tctccttttt catttgccaa
      961 aactgatcca tttgttgtat tcatgttttt gttttccatc tttgtttcct cctatgattt
     1021 aaaaaattga tcaactattc ctgactgttt caaataaaaa ccgattcctg cgccaattgc
     1081 tgtcatgatt atgatcaaca atgtcttgaa aaatccaatc gttaaccata aaacagctag
     1141 cagtagtcca agacccccaa agataatagg gagcttgtat gctgaaaata gttctttcat
     1201 tatttggccc tcctattcca ctcgagagtg cttgtagtct cttgtatctt cttgttcttc
     1261 ataaccgctg tatgtgactg ccactttgac cgtttctttt gttccgagta ctttagttac
     1321 ttcttcttcg atatcttgca tcaatacacc tgttttccct actaatgaag aagttcgttt
     1381 caagtctccg ttgacatgga tatggattcg atttttcgtt gaacgtatcc gtactttagg
     1441 tgaatgaatg aactcttctt cgtgtaaatg ggaacgtacc aacccttcga tcgctttttt
     1501 gtcgattgtt agtttgccat cttcccgttt cataacgaaa gttcctcttg actttggata
     1561 gaaaagaacg actagcaaga tgataatagc aagaacggca aatactacag ctgcccagaa
     1621 aatatattgt tgcatgtagt aattcgttaa agtaaagaag cgaaatcgtt ccaattgttc
     1681 aggcataata tacggcgcat tgatcaacgc aatcgctaga aatactgaaa gtagcagtaa
     1741 actcatgata acaccgatta tttttaaccc cttattcatt ttccacctcc tataaactta
     1801 tttttaccct gtaaataact ctaccgacaa tcacagtata gatagcgaac agttagcata
     1861 gcatcagtaa ataaagcgct accaaacatt gctggatcat aacggcgttt tattgccagt
     1921 ctgatactga aataacaatt gcttacttat tctctattta cttttgattg ctggctatac
     1981 ttaaaggata ttattcaaga ttaaaaattg caaagattat gctcagttat tattacaaga
     2041 ttctatttat ttattaaatt ttttcggaga attaaaaaat acaataaata actttagtta
     2101 ctttaatgcg ttcttacttt atttttatat tatcattttt cttattcact tctttggttt
     2161 atactattct tataaataaa aatgaggtat gtcctatgat gaaaagattg tactattcat
     2221 tgatcataac gattggctat ttgattgtat caaatctagg aaatatggta tttggtatca
     2281 gtaaagaatt cagttggact actacattat gggaatctct attctttttc atcttcgtat
     2341 ttttgttaca aaattatcga aaaaaataaa catgtgccat ctatctgttg gcacatgttt
     2401 taggatttat ggtacttttt attcggcaat ccattttatt tttctacttc ttcttaatac
     2461 ctctaacact tttgatttat aaactaatcc aatgcatcta cttgcgctta ttaagctatt
     2521 tcagctagat agtaccaccc catgcgtcaa tgcttgattt tccttttgta taacattgta
     2581 taagcaataa aggacaaata agtggtgatt acaccaaacc attgaataag aaaaattctg
     2641 atacgtaaat aaacatgaac ttgaaaaaat taatgaatcg gttagctact acgtagttgt
     2701 cttttttctc atacagttct gttaacttac acttctcttc gctagataat aaaaaatcct
     2761 ccaactgtat tgtccaatat aatcccaggc atctaaacta gcattataga agagacacac
     2821 atagaatcta aaataactgg ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc
     2881 actccacacc ttcatactct atcatttttt ataaacgata tatttttatt gtttttcgat
     2941 aaaaaatagt tgttttacaa taattattct gctatatttt agctatcaaa gataaaagag
     3001 ggataaaaat gaaatttaaa acattgctat taaaatttgt cgttttaggt ataagtgccg
     3061 tatttatttt gttcggcgta cttttttata ctcagatcgc tacaagcgaa aaaggattca
     3121 attttgattg gcagaccaca ttatttgttt ctgtcattta ctttgccctt ctcctaggga
     3181 tcgttattgc ttatttctta ttgaaactat tgaaaaaaat cgaagataac caagcttttt
     3241 ccactcaaag cttaaacata ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg
     3301 ccttttttgg cttacttcct aaatttatga aagtagcgga tttagacgat gctccaggtt
     3361 tgatgttggt aggaatcgct gtcgtattca ttccttttgc agtatacaca ttaacaagcg
     3421 tgctggaaaa actgcttgaa catgcgattt tacttaagac agatcatgat ttgacagtgt
     3481 gaggtgcgac atggcaatta tcgttaattt agatgtagta ttagctcaaa aaaaaatgag
     3541 tgtgactgaa ttgagtgaga aagtgggcat tacgatggca aacatctcta ttctaaaaaa
     3601 tggaaaagca aaagccatcc gtttttccac tctcgaaaaa atctgccaag ttttggagtg
     3661 tcaaccaggt gatttgttga cctataaaaa tgactcgaat actataccag aagaataaaa
     3721 taaagtagaa caaacagtaa ataaaaaata atctgaatga tagatgtgtg agacttttag
     3781 aagttattta cgtctattat tcagattttt tatttcgatt gaataccgtt ttatctataa
     3841 ataagatatg tactgactca tacaaagggg gccttttctt ctccatgtac tatcactttt
     3901 cctgatacaa ctggtttcct gttacgttgt agttggatct gcttttagta taatacttta
     3961 ttcattccac cagactgata gcagtttgag cagtctaaat aatcatgtag tattacaggc
     4021 cattctgttt cataccttga agtg
//
LOCUS       DACCQI010000163         3966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCQI010000163 DACCQI010000000
VERSION     DACCQI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.172"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IVO76_002639"
     CDS             5..1150
                     /locus_tag="IVO76_002639"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP7430567.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLATSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IVO76_002640"
     CDS             1321..1779
                     /locus_tag="IVO76_002640"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7430568.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IVO76_002641"
     CDS             1938..2408
                     /locus_tag="IVO76_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7430569.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            2541..>3966
                     /locus_tag="IVO76_002642"
     CDS             2541..>3966
                     /locus_tag="IVO76_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HAP7430570.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
BASE COUNT         1335 a          721 c          729 g         1181 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagcgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct cagactgtag
     2461 acaaactcct aaattgggag tgttgtttgc agtttttttg ttttatactc attgtataaa
     2521 ctattctggg agtgattcta atgatgagaa aacaatcgat tgaaggtaga aatcaatttg
     2581 ctatgttaac aattgatgat cttgtaccta aagatcactt agttcgaaag attgatgctg
     2641 cgattcagtt tgatttcatt tatcccattg tagaatctac gtattcgacc catggtcgtc
     2701 ccagtattga tccagtggtc ttaattaaat tagtatttat ccaatattta tttggtatcc
     2761 gttcgatgag acagactatt aaagacgttg aaacaaatgt tgcttatcgt tggtttttag
     2821 gttattcatt cgaggatcct atcccgcatt tctctacttt tggtaaaaac tatgttcgaa
     2881 gattcagaga aacaacttta tttgaagaca ttttctctca tattctagaa caagctgtaa
     2941 aagctggctt cgttacggaa gacaaccttt atattgattc tactcatatt aaggcaaatg
     3001 ccaataaaca taagttcaca aaagaaatga cctacggtca agctaaagct tatcaagatg
     3061 aattagaaga tgaaataaat aaagaacgta ttgctgtagg aaaacgtcct tttacatggg
     3121 acacagaaag tgagttgagt cttcaaaaaa taagtcattc agatccagaa agtggttact
     3181 atgttaaagg tgaacgagaa aaacaatttg cttattccgc gcatacttcc tgtgaagata
     3241 atggttttat cttatctaca ttaatcacac caggaaatgt acatgacagc caagtcgcaa
     3301 tcgatctggt caaacagtcg aaaagagctt ttccaaatat aaagcgtgtt gtcgctgatg
     3361 caggatataa gacgcctaaa tttgttcatt ttctgtcaca cttgaaactc tacccgattc
     3421 ttccatatac tgtacctcat ggagtaaaag gaatgtttcg taaaaaagac tttgtttatg
     3481 atgcctattt tgactgctat ttatgtccca ataatcaacc gttgttattt tcaactatca
     3541 ctcgtgatgg ttatcgaacc tataaatcaa atccaaaatt ctgtaggagt tgtccattac
     3601 ttaaaaactg tactttgagt acaaaacatc aaaaaatagt aactcgacat gtttgggcgg
     3661 atcttatgga tgaacttgaa caccaacgcc atacggcgtt gaatagagaa atttataaaa
     3721 agagaaaaca aaccattgaa cgaatatttg cagatgcaaa agaaaagcat ggtatgcgtt
     3781 ggacgaaata tcgaggtctc gaaaaagtcg ccacgcacac gatgcttacg tttgctgcca
     3841 tgaatttaaa aaaactagcc acatggctat ggaaagcaaa aaagcctatg gctttttatt
     3901 taaaatttct acaaagacta acaaaaagga gttggcaatt aagccactcc tttttgtcta
     3961 cagtct
//
LOCUS       DACCQI010000164         3958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCQI010000164 DACCQI010000000
VERSION     DACCQI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.173"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(152..427)
                     /locus_tag="IVO76_002643"
     CDS             complement(152..427)
                     /locus_tag="IVO76_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7430571.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(399..1352)
                     /locus_tag="IVO76_002644"
     CDS             complement(399..1352)
                     /locus_tag="IVO76_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7430572.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1964..3457
                     /locus_tag="IVO76_002645"
     CDS             1964..3457
                     /locus_tag="IVO76_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7430573.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3592..3888
                     /locus_tag="IVO76_002646"
     CDS             3592..3888
                     /locus_tag="IVO76_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7430574.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
BASE COUNT         1324 a          645 c          712 g         1277 t
ORIGIN      
        1 atttagtata gtaaaaacta gaaacaataa aatttgtgaa tgttgtatac ccccaaacaa
       61 tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt atactaaata
      121 tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta ttatatataa
      181 tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg actatttcat
      241 acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg ggaggatcta
      301 cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg acattaatat
      361 catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc gttggtaata
      421 atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat tgagtcagca
      481 tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta ttcaattctt
      541 tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc tacataatca
      601 tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt aacaatgctt
      661 ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc gtgttgagag
      721 gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg agactcttca
      781 aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc ttccattgcg
      841 taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa tgcacgaacc
      901 acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt tctcaattct
      961 tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt ttcggacaag
     1021 tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg tggacttact
     1081 tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga tcgatcaaag
     1141 ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg gtcataatca
     1201 attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa atcattgtct
     1261 gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc tagtttatct
     1321 gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcct atactaaata
     1381 tatttagtat acttcatata cttaatatat tcgatatatc taatatactc caaattgata
     1441 agaattcaga ctttataagg aatatagtgg atatactaaa tatatttgct atattcagta
     1501 tatcagatat attcaaaatg tcaaaatata tttgtgcgtt ttgcgttgac aatttagagt
     1561 gaaaagaggt aaacttaatt ctatagaaac cacagataat tcactattta caaagtagca
     1621 aaagcagtgg tactctttaa acagaaaaat tacataaaaa aaagagacgg aaataattga
     1681 ttttagcaga atcaattatt tctgaatcac atggttagga catgtgttca cgctcgtctc
     1741 aagaatatgt aagtagctac caactactta tattaaaagt tgatcggata ccttttccga
     1801 ttgcctttat tctaccaaaa aaaggcgagc aactcaacag aagaaagttt atttagtgta
     1861 cttaaattaa atattcttaa acactgaaga gagaacacat actatggctg agattaaagc
     1921 taaactgtgt tctctttttg tttgagaaag gacgttacaa tcgatgttga gcgaaattta
     1981 ttcagctatt cttaaaaacg gcttacgtac atacaaatat cgaaacagca ccatgaaacc
     2041 tgcaggtcat attgattcca gtaggagtgg tgctatcttt gcgtaccgta gcaagcaatt
     2101 aatgaatgtt gggcgtggta tggtcattac ttcggaagaa gctattttag agaacgaaaa
     2161 gaaactaacg cattggacac ctaataccta tcgctttggt acttatgcgg atgattatcg
     2221 aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa atcaacacat ttgtagttga
     2281 tatcgattca aaagagaacc atcaaggcga aattatttta gcctgtttag atcaagtggg
     2341 ctacatgcca acgctaattt tagaatcaga tcatggttat caagtttatt ttgtactaaa
     2401 gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg atcgatgtag caaaaaaaat
     2461 ttcaaaaaca attcgtctac agttggctga aaaattgtcc ggagtggatc taggatgtaa
     2521 ccacttcgga attgctcgat tcccacaaga aaataatatt gtttttaaag aaatgaacta
     2581 tcagtattct tttgctgact ggattcaatg gtcgatgaag cttgaatcag atcaaaaaac
     2641 aaaagaagat cgagaacgaa aactaattgt ctttcctgag aaaaaagaaa ttcgtcaagt
     2701 agatgaaccg tggttcgata tgttacttca taaagcaaac atcgtcggcg gagaaggacg
     2761 tcttggtcga aataatgtga ttttcacgct ttctttagct tattactctt caggttatgc
     2821 attggatact tgcgaataca atatgttcac attcaacgaa cggttacagg aaccgctatc
     2881 tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct ggtaattatc aagcagcaag
     2941 cagagaattc attattgaat tgtgccagca atgggtttca ccaaccttaa aaaataatga
     3001 tctgttcatt caacgacgtg gctggtggaa atttaaaaag ccacgagatc aaagaaaata
     3061 tagtcataaa aatgaatgga aagaagatct attggcttat ctaagcgaaa aaagtcttgc
     3121 tagtgagcct tatttgaacc tgtcaaaaaa agaattggct tcacaactaa cgatgcccaa
     3181 gcgaacactt gataagatct taaatgagtt acgagaggag aataaagtct tctacaaggt
     3241 aacacgagga agaaatggcg gaatgatcat tgcttcagtc aaaacgttgt tcgcgagggt
     3301 tattcaacta agtaaagaaa agagactaga ttattcaaca aggatcacag aggtatttgg
     3361 actcaacgag agcttagtag aagagacatt tagacaatta tcaaccccct tgaaccctct
     3421 taaagaagtg aatctttttg aagtagatac aggttgaaaa ataaaaggtg caaattgccc
     3481 tatacaatat atctatgata cttctgtagg tgttgctccc tttgcctagc tacgtgtagg
     3541 tggtatactt ataactgcgt atgcagtctt atgtatcatg gaggaagttg tatgtcgtta
     3601 aaaaattatg tctattcaca tcctgttaat gtgtttatca ttggtcgttt gggattatct
     3661 gtggaagtgt tctgtgagct ttatggtttt aagcagggga ctttgtcttc ttgggttacc
     3721 agggaaaaaa cagttgcttc tttgccgatt gagtttcttc atgggttgtc cttagcttct
     3781 ggggagacta tggatcaggt atatgattgt ttgtgtgtgt tagagcagga gtatattgag
     3841 tataaaatag ctaacgagtt aagaaaaagg aagaagtata tccagtagta gtttttattt
     3901 acgccgtttg tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaat
//
LOCUS       DACCQI010000165         3909 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCQI010000165 DACCQI010000000
VERSION     DACCQI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.174"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            108..3149
                     /locus_tag="IVO76_002647"
     CDS             108..3149
                     /locus_tag="IVO76_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7430575.1"
                     /translation="MIHLVENKEIRFEQDIETSLTTYGKWKKETFNESNFDPIIGLDC
                     QKLLGFVQKTQPKQWARHQRNYNNDGEVKFLQRFNQEVETHGMLHVLRNGIKDRGVRF
                     KVAYFKPASELNDETMEKYEANSFTCTRQFAYSPNNHKTIDMVLSLNGIPLVALELKN
                     QYTGQTVENAKLQFQKDRDPKELVFRFNRRFLVYFAVDHTDVYMTTKLDGKKTYFLPF
                     NQGSNGPGRVGGAGNPSNKDGYPTAYLWEKVLTRDNLMDIIQRFMNLEIKKEKQGDRE
                     VTKKTLIFPRYHQLDVVRKLVADVKSHGSGENYLIQHSAGSGKSNSIAWLAYHLASIH
                     DQLNYPIFSSIIIVTDRTVLDRQLQNTIMNFDHQTGLVETIDDRKSSQDLKEAINDGR
                     KIIITTLQKFPVIYQEVDQVEGRNFAVIVDEAHSSQTGNSAQKLKAALSDKSKSLEEY
                     YELEGEVDDVLPDAEDELVQQLVAQGKHQNLSFFAFTATPKEKTLEMFGTKQPDGHFE
                     PFHVYSMRQAIEEGFILDVLKNYMTYSTSYKIAREIEDNPELPKSEATRAIVRFQSLH
                     PWAVTQKTAVMVEQFRAVTRTAIGGQAKAMVVTASRLHAVRYMKEFKKYIANKGYNDL
                     DVLVAFSGSVVDGETEYTETKMNVTKSGENVRENQLKEAFHTDNFNILIVAEKYQTGF
                     DEPLLHTMFVDKKLKGVKAVQTLSRLNRTMPGKKDTFILDFVNDVEDIEAAFQPFYEQ
                     TNLREEINVNLIYDTQSKLRKFNVYNQGDIERVMKLVKQAQKQQNERLLGRISSMFKP
                     IMDRYEEMNKDNQYQFRVTLRNFGKWYDYISQLDRLFDMELLEERIFTGYLLKFLPKE
                     TRENIDISDKVKLEYYKLRQDFSGEIHLVNKEGELVNPKSIDAGIKPPDERDSLEEII
                     HKINERFPNDFDEGDRVLVEILYRSFADNPDLKVVSMAKNNDAQMFEKSLFPDVFKDK
                     VMEQYEKNNAAFEKMFGHDDKYFDLVYTLVARDLYKLLRTK"
     gene            3339..>3909
                     /locus_tag="IVO76_002648"
     CDS             3339..>3909
                     /locus_tag="IVO76_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HAP7430576.1"
                     /translation="MSTTIEVNKQSVKQFLETGKIKKFVIPEYQRPYAWTDEQNQVLF
                     DDLAEYTANNNESTYFLGSIVAYENDHNEQEIIDGQQRITTLFLFLRAIYAKLENSCE
                     KEALFLKSQIEPALWEQDDLTGEVKPDKILIMSRVMGDEGNEEFASILVSGEADVKSK
                     SNYSKNYILIQHLLNEYATNEPLSFYRFIS"
BASE COUNT         1343 a          622 c          827 g         1117 t
ORIGIN      
        1 taataaatca aatgattgat aataagatag ctgaattgat ggaacttgag agttataaaa
       61 aagctctcat ctacgaatac gtaaccggta aaaaagaagc ctaggaggtg attcatttgg
      121 tagagaacaa agaaattcgt tttgagcagg atattgagac ttcactgact acgtatggca
      181 aatggaagaa agaaacattc aatgaatcaa actttgatcc cataattggt ttggattgtc
      241 aaaaattgct aggctttgtc cagaagactc aaccaaaaca gtgggcacgt caccaacgaa
      301 actataacaa tgatggagaa gtcaagttcc ttcaacgctt taatcaagag gtagaaaccc
      361 acggcatgtt acatgtattg cgcaatggga tcaaagaccg cggtgtgcgc tttaaagtag
      421 cttacttcaa acctgcttca gagctgaatg atgaaacgat ggaaaagtat gaagcaaata
      481 gttttacctg tactcgccag tttgcttaca gtcctaataa ccataaaacc attgatatgg
      541 ttttgtcgtt aaatggtatt ccgctcgttg ctttagaatt gaaaaaccag tacactggtc
      601 aaacagtaga aaatgccaaa ctccagtttc aaaaagatcg tgatccaaaa gaattggtat
      661 ttcgttttaa tcgccgcttc ttagtctatt ttgcggtgga ccatacagat gtttatatga
      721 cgacaaagtt ggacggtaaa aaaacgtact tcttgccatt caatcaaggt tccaatgggc
      781 cgggaagagt tggtggagca ggcaatccgt ccaataaaga tggctaccca actgcctact
      841 tgtgggaaaa ggtgcttact agagacaatt tgatggatat tatccaacgt tttatgaatc
      901 tagagattaa aaaagaaaag caaggcgaca gagaagtcac aaagaaaacg ttgatttttc
      961 ctcgttatca tcagttagat gtggttcgaa aattggttgc agatgtaaaa agccatggta
     1021 gtggagaaaa ttatttgatc cagcatagtg ctggttctgg aaaatctaat agtattgcat
     1081 ggctggcata ccatttagcc tctattcatg atcaactgaa ctacccaatt ttttcatcaa
     1141 ttattattgt gactgatcga acagtgttgg atcgccagtt gcaaaatact atcatgaact
     1201 ttgatcatca gacaggtttg gtagaaacga ttgacgatcg caaatcctct caagatttaa
     1261 aagaagccat taatgatggg cgtaagatca ttattaccac tttgcaaaag ttcccagtga
     1321 tctatcagga agtagatcaa gtggaaggtc gtaattttgc tgtgattgtg gatgaggcac
     1381 attcttcaca aactgggaac agtgcccaga agctaaaagc cgctctttcg gacaaatcta
     1441 aatccttgga agagtattat gaactggaag gcgaagttga cgatgtatta ccagatgctg
     1501 aagatgaact agttcaacaa ttggtagcac aaggtaaaca tcaaaattta agtttctttg
     1561 cttttaccgc tacaccgaaa gaaaagacat tggaaatgtt tggtacgaaa caaccagatg
     1621 ggcatttcga accctttcat gtttactcga tgcgccaggc gattgaggaa ggctttatct
     1681 tagatgtgct gaagaactat atgacctata gtacttccta caaaattgcc cgtgaaattg
     1741 aagataaccc agaattacca aagagtgaag caacccgagc gattgttcgt ttccaatcat
     1801 tgcatccttg ggcagttact caaaagacgg ctgtgatggt tgagcaattc cgagcggtta
     1861 ctcgtacagc tattggtggg caggcaaaag caatggtcgt taccgcatca cgcttacacg
     1921 ctgttcgcta tatgaaagaa tttaaaaaat atatagcgaa caaaggttat aatgacttgg
     1981 atgtgttggt tgctttttct ggttcggtag tagatggaga gactgaatat acagaaacga
     2041 agatgaatgt cacaaaatct ggtgagaatg ttcgggagaa tcaattaaag gaagctttcc
     2101 atacagataa ttttaatatc ttgattgttg ctgaaaaata tcaaacgggt tttgatgaac
     2161 cgttgctgca taccatgttt gtcgataaga agttaaaggg tgttaaagct gttcagactt
     2221 tatcacgact caatcgaaca atgcctggga agaaggatac ctttattctt gattttgtaa
     2281 atgatgttga agatattgaa gctgctttcc aaccctttta tgaacaaaca aatttgagag
     2341 aagaaatcaa tgttaacttg atttatgata ctcaaagcaa gctacgaaaa tttaatgttt
     2401 acaatcaagg tgatattgaa cgtgtcatga aattggtgaa acaagcccaa aagcaacaga
     2461 atgagcgctt gcttggacga atttctagca tgtttaagcc gattatggat cgctatgagg
     2521 aaatgaacaa ggataatcag taccagtttc gggtgactct acgtaacttt ggcaagtggt
     2581 atgattatat ttcacaactt gatcggttat tcgatatgga actattggag gaacgaattt
     2641 ttactggata cttgctgaag tttctaccta aagaaacacg agaaaatatt gatatttctg
     2701 acaaagtaaa gttggaatat tataagttac gtcaggactt ttccggtgaa atccatctag
     2761 tgaataaaga aggcgagcta gtaaatccaa agtcaattga tgcaggaatc aaaccgccgg
     2821 atgaacgaga ttcactagaa gaaatcattc ataaaatcaa tgaacgattt ccaaatgatt
     2881 ttgacgaagg agaccgggtg ttagtagaga ttttgtatcg gtcatttgcc gataacccag
     2941 atttgaaggt tgtcagcatg gctaagaata acgatgctca gatgtttgaa aaatcgctgt
     3001 tccctgatgt atttaaagat aaggtgatgg agcagtacga gaagaacaat gcagcttttg
     3061 agaaaatgtt tgggcatgat gataagtact ttgatctagt atatacatta gttgctagag
     3121 atttatacaa gttattacgg acgaaataaa atttataaat aaggattaga caatacaatt
     3181 tctctcaaaa ataaaggaga agttgtattg tttgctttta agaaagccct gaaaaatttg
     3241 ttacgtacta aattgtctga actctgtcat atcagctatg tcagaataaa ggaaatagat
     3301 ttatttttat aaacagtgtc gtctcggaga tttaaactat gtcaacgaca attgaagtga
     3361 acaaacaaag cgtgaagcag tttcttgaaa cagggaaaat caaaaaattc gttattccag
     3421 aatatcaacg tccttatgcc tggactgacg agcaaaacca agtactattt gatgatttag
     3481 cggagtacac agcaaacaat aacgagagta cttacttttt aggaagcatt gtggcttatg
     3541 aaaatgatca taacgagcaa gaaatcattg atggacagca aaggataact acgctatttt
     3601 tatttttgag agctatctat gctaaattag aaaatagttg tgaaaaggaa gctttattct
     3661 tgaaaagcca aattgaaccc gcgttatggg aacaagatga tttaacaggt gaggttaaac
     3721 cagataaaat tctgattatg tctcgagtaa tgggggatga ggggaatgaa gaatttgcta
     3781 gtattctagt aagtggtgaa gcagatgtta aatcaaaaag taattattcg aagaactata
     3841 ttttaattca acatctatta aatgagtatg caacaaacga accactatca ttttatcgtt
     3901 ttatcagta
//
LOCUS       DACCQI010000166         3895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCQI010000166 DACCQI010000000
VERSION     DACCQI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.175"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..110)
                     /locus_tag="IVO76_002649"
     CDS             complement(<1..110)
                     /locus_tag="IVO76_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7430577.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVT"
     gene            273..1103
                     /locus_tag="IVO76_002650"
     CDS             273..1103
                     /locus_tag="IVO76_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7430578.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            1087..2454
                     /locus_tag="IVO76_002651"
     CDS             1087..2454
                     /locus_tag="IVO76_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7430579.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHKVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            2473..3282
                     /gene="yidA"
                     /locus_tag="IVO76_002652"
     CDS             2473..3282
                     /gene="yidA"
                     /locus_tag="IVO76_002652"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7430580.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(3325..3768)
                     /locus_tag="IVO76_002653"
     CDS             complement(3325..3768)
                     /locus_tag="IVO76_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7430581.1"
                     /translation="MDKPIFGEKKQSANYQTRYAAYIVIERDKRIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
BASE COUNT         1217 a          791 c          724 g         1163 t
ORIGIN      
        1 attgtcacca tttgcccttt caaatcaaaa taaaaatctt gcacttcccc ttgctgtttt
       61 acttttgtac gcaatttgat tgaaatcggt gttccttgtg ataattccat tattcattct
      121 cctttacttc tcatagctac attgttccta gtttcttttg ttttgtcaac caacttgtcc
      181 atttttacag aaaacctttt ttccaaaaca actctttgat tttagagtat aatattgtag
      241 aaaatatgaa tttatacaaa ggagccggtt gaatgaaccc tgcgattcga ctagatcagc
      301 acatttatca aaaaaacgac ttttcccctt ttgcattaac agatatatta acggaatatt
      361 ctaaagaaca ccagcaaatc tttttacatt tctggcaata tgactgcaca gtcatcttag
      421 gtatgaagga tgttcgaact ccttttctaa gtgacgggat ccaaacactg aaagatgcag
      481 gttacgctcc agtgatcaga aattcaggtg gattagggat cgtatcagat gaaggaattt
      541 taaatatttc tttgattttc cctcaaagtg acacaaaaaa aataaccata gatgacgctt
      601 acgagcaaat gctcctattg actaaatacg cttttccaaa ggcaccgata caagcattcg
      661 aaatcaccca ttcttattgt cctggaactt atgatttaag catccatggc aaaaaatttg
      721 ccggtatcgc tcagcgtcgg atcaaaaatg ggatttctgt tatgatgtac ttaagtgtca
      781 acggtaatca ggaaaaacgc gggcaagtca tgaagcgatt ttatcaatct gctttaaaag
      841 aagactttgg tacaaatggc tatcctgcag tggatcctgc tacaatggca acacttgaag
      901 aactgcttga ccaacccctt tttgtaaaag aagtcaaaga agcatttgca aaagcttttg
      961 aaaagctgta ttctgcaagt gatttacagg attctaccga ctggctaaaa aaagaaatcc
     1021 agctggagca atggaacaac caaatccaga gaatggaaca acgtaataac ataaaggagc
     1081 tacattatga caacccctta taaatatcaa atgctgccta tggaaaaagt ttttcgtgat
     1141 ccagtccata actatatcca tgtccagcat aaagtaatct tagatttgat caattcgaaa
     1201 gaagtccaac gtttacgacg cattaaacaa ttaggcactt cttctttcac ttttcacgga
     1261 gcagaacata gccgttttac ccattcatta ggcgtttatg agatttcaag acgaatctgc
     1321 gatatctttt ctcgaaattt ttcaaaagaa aaaataggca acggaggctg ggatgatcag
     1381 gaacggctgg tgactttatg tgcggcactg ttgcatgatg tcggtcatgg tccttactct
     1441 catacatttg aacatatctt ccatacgaat catgaagcca tcactgtaga gatcatcact
     1501 tctccagaaa cagaagtgta tcagattctt aaccgagtag aagaaggttt tcctgaaaag
     1561 gtagctagtg tgatccaaaa aacttacccc aacccacaag ttgtccaaat gatttctagt
     1621 cagatcgatg cagatcgaat ggactatttg cttcgggatg cttatttcac tggaacagaa
     1681 tatggaacat ttgatcttac ccgaatatta cgtgtgattc gtccttatga aggcgggatc
     1741 gctttttcca tgagtggcat gcatgcagtg gaagattata tcgttagccg atatcaaatg
     1801 tatgtgcaag tgtacttcca tcctgtttcc cgtggtatgg aagtgatttt ggagcatctg
     1861 cttcatcgcg cacacgagtt gtatcaagaa gatgccagca gttttgaact ccattctcaa
     1921 ttacttctgc catttcttga cggaagcttt actttagaag aatacctcaa acttgatgat
     1981 ggggtcttgg gcacttactt tactcaatgg tctgaatctg ctgatcctat tttaaatgat
     2041 ttagctaaac gttttttgaa tcgtaaacca ctgaaatctg ctactttttc tggcaaccgg
     2101 gattcaaaat tagtccaaga actcactttg ttagtagaaa aggttggcta taaccctgtt
     2161 tattacactg ctgtgaattc tagctacgat ctgccttatg atttttatcg cccagaacaa
     2221 ggcagacatc gcacacaaat cgagattttg cgaaatgacg gaactttgat agaattatca
     2281 caggttagtc agcttgttgc tgcattagcg gggcaagaac aaggagatga gcgatttttc
     2341 tttccaaaag aaatggttga tccctccttg cgtgatcatt atgatttatt tgacgaaact
     2401 tatcaagaat ttgccagcca tatccgtaat ggcgcattga ttgaaataaa ttaaaatata
     2461 ttggaggaaa ttatgtctat caaactagtc gctatcgaca tcgatggtac tttattaaat
     2521 gagaaaagag aagtaacaaa agaagtaaaa gaagcgattg ccgctgctgt tgcaaaaggt
     2581 gtttcgatcg ttttatgcac tggccgtccc ttaccaggtg tccaggaaca gttaaacgag
     2641 ttgaaccttt ttcaagataa tgattacgtt attacttata acggggcact cgtacaacaa
     2701 acgaaatcag gtaaaatcat cgcccgtcac ggactgactc atgaagattt cctagagatc
     2761 gaagtgatgg cacgccgtgt cggcagtcat ctccattcga tcgatgacaa agcgatctac
     2821 acgcctaatc gagatatcag cgcttatacg atccacgaag caagtttagt caagatgccg
     2881 ctaaaatatc gaacacctga agaaatgaca ccagacatga acatcgtcaa aatgatgatg
     2941 atcgacgaac cagaaatttt agatgcagca attgcgcgtt taccgcaaac tttccgtgat
     3001 aaatacacaa cggtcaaaag tgcgccctat tacttcgaag tactaaataa agaagcaagt
     3061 aaaggtgcgg cagttgcgaa tctcgcacag caccttggta ttgatcaaga tgaaatcatg
     3121 gctatcggag ataacgagaa cgacttatcc atgattgaat atgctggtct aggagtagca
     3181 atgggaaatg ccgtttctct tgtcaaagaa gcagcaaatg tcatcacttc atcaaacgac
     3241 gagcacggtg tagctgaagc aatcaaaaaa tatgtactgt aataaataaa aaaactgaga
     3301 gaaagctgat ctactcgctt tctttcagtt tttatttttt tcgatccatc tctcgactgc
     3361 ccatcggtgg ctaccacgtt tcaatttcac aagcgcttct tgagggctca cccaccaaat
     3421 tttattggtc ttttcagttg gttctcctat acgcttccaa gaatcagcaa caaagaaata
     3481 tcctggatta taataataag ttgcacgata attcgagtaa aaatactcat cagcttctcc
     3541 cagataagaa gaaatttctg ccacaattcc catttcttct atcagctcac ggacaatcgc
     3601 ttcttctttc gtctcttctc cttcgatttc cccaccagga aggaaaaaag caccatttgg
     3661 agcctcgatc accgcaatcc gcttgtctct ttctatgaca atatatgctg cataacgggt
     3721 ttgataatta gcagattgct ttttttcacc aaaaattggt ttatccatga attgtttcca
     3781 ccttaacagt attattttct atccatttta catgagaaat ttctcatgaa caagcacaaa
     3841 tcaactattt tcttacttct ttttgttatg attgagtaaa aggaaaagga gagat
//
LOCUS       DACCQI010000167         3875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCQI010000167 DACCQI010000000
VERSION     DACCQI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.176"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            393..2486
                     /locus_tag="IVO76_002654"
     CDS             393..2486
                     /locus_tag="IVO76_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7430582.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            2646..3512
                     /locus_tag="IVO76_002655"
     CDS             2646..3512
                     /locus_tag="IVO76_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7430583.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(3534..>3875)
                     /locus_tag="IVO76_002656"
     CDS             complement(3534..>3875)
                     /locus_tag="IVO76_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7430584.1"
                     /translation="AEMKFETMTPELAKEHYQHLTERSFFDELIDYMTSGPVVYLVLV
                     GEEVIDIVRKMVGATKAADAVPGTIRGDYALPGTENIIHASDSRDAAVKEIARFFPPF
                     DINKPSEKAAK"
BASE COUNT         1289 a          665 c          781 g         1140 t
ORIGIN      
        1 gaacaatttc aaaaaacagc ttctcatgtt ttataaatat tgtgacttgt ggccgaggag
       61 ttggcaggag aacaccgttt aaggaagaag ttgtgaaaaa gctgtcctgc atcaagaaat
      121 aaggagctgc gacaagaatt ttgtcacagc ctcttttttt gtacgaatac cgagaaactg
      181 gcttttgaat accgtggagt gaagcggtca ccacaagact tttatctctt cattagttta
      241 atcgcttaca tcgcagcggt tttatgctgt gaaaagtgga cggaataatc tttagaaaga
      301 ataaaaaaat cttaagataa caactttttt aggaaatttt catttttcag gagcaaaata
      361 agaaaaaatg tgaatagaga gaagtgatat acgtgacaca acttaaaaaa ttgtgccaaa
      421 atcgtttagc aatcgttttg acagctgttt tctttttctg gctgaagacg atcattgcct
      481 actatgcaga tttttcatta ggtgtggaag gtacgatcca gtactttatc ttgtggatca
      541 acccaattgc gactacctta ttgttttttg gcctttcttt gtatattaaa aagccaaagc
      601 cagcgttagc tattcttttg atcattgata ttttaaatac gcttttgttg tacttgaata
      661 ttatttttta tcgggaattc acagatttca ttacagtcaa atctgtatta ggcttttcga
      721 aagtgtcgca aggattgtca ggaagctcct tttctttgat gaagccccat gatgtcatct
      781 actggctgga tatcgctgtg ttcattgggc tactggtttg gttgaaggtc aagaaaatac
      841 ctatcaaaag caaccctgtc ggcaaaccga tggctatcgc tgttacctgt ctttcagggc
      901 ttattttttc aggaaatcta gcgctgagcg aagccaaccg tccacaattg ctgcagcgaa
      961 catttgaccg ttcatatatt gtcaaatatc taggaatcga tgcgtatact atttatgacg
     1021 gaatcaaaac aggaatgact agcagtgttc gcgcgcatgc aagcagtaat gggattgatg
     1081 aagttttaga ttatacaaag aaacattatg cagagccaaa tcccgaaaca tttgggatcg
     1141 ctaaagggaa aaatgtgatc gttctccatt tagaaagttt ccagcagttt ttgattaata
     1201 tgaaggtaga cggacaagaa gtcacaccgt ttttaaatag tattttccag aatcaagcaa
     1261 cgatcagctt tgacaatttc tttcatgaag taggacaggg aaagacaagt gatgctgaaa
     1321 atatgttgga aactggcaca tttggtttac ctcaaggctc attattcaca gaacttgggt
     1381 cagacaatgt ctttcaagcc gcaccagcga tccttggaca aaagcaaggc tatacaagcg
     1441 ctgttttcca tgggaatgta gctagttttt ggaatagaga tcatgtatat aaaaacttag
     1501 gttatgacaa tttctttgat cgatcttatt ttgatgaatc cgacgaaacg ttaggttatg
     1561 ggatattaga taaagattta ttcagagaat ctgcccaata tcttgagcat ctacaacagc
     1621 cgttttacac gaaatttctt tccgtaacca accacacgcc ttattatacg gatgacaaga
     1681 atttcgactt tccatctttg aatactggca atagcacagt agataattat gttcgaacgg
     1741 cacattatct ggatcaatca ttggaacaat tttttacgta tttgaaaaaa tctggtattt
     1801 accaaaattc aatctttgtg atttacgggg atcattttgg gatctcgaat acagataata
     1861 aagatttagc aagcgctctt ggtaaagatc cagatacatg ggatgaattt gacaacgcac
     1921 agatgcaacg agtgccattg atgatccata tgcctggtta tactaaaggg acagtcaatc
     1981 atgaatacgg tggagaaatc gatgttttgc cgacattact acatctattg ggaatcgatg
     2041 ataaagatta tcttcatttt ggtacagact tattatcttc tcagcatgat caagtagttg
     2101 cttttcgaaa tgggaacttt gtgacaccca aatacaccgt cctaggagga aaagcttaca
     2161 ataatcagac aggagaaatc atcgatacga aagcagcagg aatcgatgag gaaatctcca
     2221 aagatcagga aaaagtcaag actgcgttaa gtctttcaga taagctgaat caggaaaatc
     2281 tcttgcggtt ttatgtgcca aacggatttg agacgattga tccgaaaaaa tatgactata
     2341 aaaatgaagc ggaacgtaat gaagcaatcg aaaagcaaaa aggcgaacat tcgacaagtg
     2401 tcttttcaaa aaatggtaat aaaacaacaa ccaacttata tccagtaaca agaacagacg
     2461 ttgaacagca aaaaaataat caataaacaa tcaaaccaac cttcagtagt tagacaacat
     2521 tctaatgctt gaaggttggt attttttatg aaaaaaacat ttttttattt ttttcgtgta
     2581 tttttccttt ttaacgtatc tatttattga atgtggaaaa atttaaatta aaaaggagaa
     2641 ctattatgta tgatggtgaa ttgatcaaga aacttcgagt caacaagaag ttgacacagt
     2701 cccaattagc ggaaggaatc tgttttaaga cttctctagt cgggattgag acaaattcag
     2761 tgaaaaaaat gtcatttgta acattgagat catttttaga gcgaatgaac atgacacttg
     2821 cagaatatga atggatgcga aaccagctgg atgaaccaat aaagttaaag aaaaaaagac
     2881 gaatgctaga tcgggtacaa gaagacagct ttgatcctta taaagagatc agcaataacc
     2941 gtaggcattt taagaaaaca gaagatctgt attatttggt tttgaatctt cacatggtcg
     3001 aaaaaacaga agtatctgag acagaatttg aatttttacg ttatgaatgc cgaatcattg
     3061 aagaatactt gatgcagata ccagactatg gtcaatttga actatctatt ctagctgatt
     3121 ttccttggat attcagtgat cggttcattg agaacaatta tttgaaaatc aaaaaaagaa
     3181 tgcgacgatt agcacagttg aacaatcatg agcagtatct ctttactttt ttaatgaacc
     3241 tgacttcgtt ttatatagaa aatggtcggt tgaaaaaggc acgtagtgtg aatgaagata
     3301 tgaaacggag cttagcacat aaagaaagaa gtacagtgat ctatgaaaca ttgatggcag
     3361 attattatca aagattgatt tctgctgctt taggggaaga aaagacgaaa gactaccaag
     3421 aattcttcac agtgctcgaa tatatgttag gaaagaatca acggaggtcc ttagagaaaa
     3481 aactgctggc cattgctgaa cccagcaact agaccagcag tttagaattt gtatcacttc
     3541 gccgcttttt cagacggttt gtttatatcg aatggcggga aaaagcgggc gatctctttt
     3601 accgcagcat cacgggaatc agaagcgtga atgatgtttt cagtccccgg cagcgcgtag
     3661 tctcctcgga tcgtacctgg aacagcatcg gcagcttttg ttgctccaac catcttacgg
     3721 acgatatcga tcacttcctc gccgaccagc acaagataaa cgacaggacc ggaagtcatg
     3781 taatcgatca gttcatcaaa aaaactgcgc tcagtcaagt gctgataatg ttcttttgct
     3841 aattcaggag tcatagtttc aaacttcatt tcagc
//
LOCUS       DACCQI010000168         3867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCQI010000168 DACCQI010000000
VERSION     DACCQI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.177"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(279..1148)
                     /locus_tag="IVO76_002657"
     CDS             complement(279..1148)
                     /locus_tag="IVO76_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7430585.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(1440..3050)
                     /locus_tag="IVO76_002658"
     CDS             complement(1440..3050)
                     /locus_tag="IVO76_002658"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7430586.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
BASE COUNT         1140 a          803 c          636 g         1288 t
ORIGIN      
        1 tggggaatca gctaagattg cagctggaat caatgcgact gcactatttt ttgcaccgct
       61 gatcgttact tcaccagcta aaggtcggtt tcctttgatt acgatttttt tcatctgttt
      121 ccaccttgtt tattaatctt ttttcatgga tatgcttgta ttttatcata gataaaaaaa
      181 agaaacgagc atggtctgcc gttttaagta aaaaagaatg gagagcgact tcaaaagtcg
      241 ttctccattc ctatatatcg tatctgttat gtggatgctt acgctttgtt agctgaaccg
      301 aaccagtcga tatgagattt agcatcttca acgattgctg ctttacctgg agctaataat
      361 ttacgagggt cgaatccttt accttcttta tctttaccag cttcgatata tttacgagta
      421 gctgcagcaa atgataattg gaattcagtg ttaacattta cttttgagat acccattgag
      481 atagcttttt cgatttgatc ttgaggaata cctgatccgc catgtaatac taaaggcatg
      541 tctggaccta cagcatcagc aattgcttgt aagtggtcaa aagctaatcc tttccagttt
      601 tctggatatt gaccatggat gttaccgata ccgcaagcta agtagtcgat acctgtagca
      661 accatttgtt tgcattcttc aatatcagct aattcgccgt taccgatgat tccgtcttct
      721 tctccgccga ttgaaccaac ttcacattca actgagatac cttttgcatg tgctttttca
      781 acaacgtctt tagctaattt caagttttct tcaaatggta gatgtgaacc atcgaacatt
      841 actgaagtat agcctaattc gatacattcc aaagcagctt cgtaatcacc atggtctaag
      901 tggatagcaa ctggaacagt gatgttcatt gcttcaacta aatcagtgat taaatcttta
      961 cagattttgt aaccacccat gtatttagct gcacccattg atgtttggat aagtactggt
     1021 gcttgttttg cttcagccgc ttcaaggatt gcttgagtcc attccaagtt gtttgtgttg
     1081 aatccgccga cagcgtaacc gcctttacga gcagctttta aaaattcagc tcctgatact
     1141 actggcataa taaaattcct cctaagtttt gtaaaactaa atttgttaag acaattctta
     1201 accaacgacc ctattttacc aaacatttcg gtagttttct actaaaatca atgaaaatga
     1261 aaattttcaa gaagtttttt gcaaaaaata aaatatccaa tagcgcttta atcgaaaaaa
     1321 gtcttttgct ttctatcttt tttcctacaa ataataacaa atcagaaaaa gagaaaagtg
     1381 acaagaaaat tgcttatccc cttctcttgc tacttttctc tttctatcaa gttcaaaaat
     1441 tattttgttt cgtgatctgc taatgatgct ccgacaaatg ctttgattaa tttttgcggg
     1501 cgatttggac gagaaatcaa ttctgggtgg aattggcatc ccacaaagaa ttttttctct
     1561 ggcagctcta cgatttctac taaacggtta tctggtgaga cacctgaaaa gactagccct
     1621 tgttcttcaa acaattgacg atatttgtta ttgaactcat agcggtgacg gtgacgttct
     1681 tgcacaacat cggctccatc atatgcttca gccgtcttcg ttcctttttt gattttacat
     1741 gggtataatc caaggcgtaa agttccaccc aaattctcga tgttttcctg atcagccatt
     1801 aaatcaataa tattgtttgc tgtatctgga ttcgtttcag ctgatgacgc atcttctaat
     1861 ccaacgacgt tacgtgcaaa ttcgacacaa gccatctgca taccaagaca aatacctaaa
     1921 aacggaacgt cattttcgcg agcataacga atcgcttcga ttttcccttc tactccgcga
     1981 tcaccaaatc caccaggaac caagattcca tctgcgtctt tcaaacgttc ttctacattt
     2041 tctctcgtta attcttgtgc tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa
     2101 ccagaatgtt tcaatgcttc aactactgag atatatgcat ctggtaactc gacatattta
     2161 ccgacaagcg caatccgtac ttttttcttc aagttcaaga cacgttcttc taattctttc
     2221 cattctgtca tgtctgcttc tggtgtatct agttttaaat ggtcgcaaac aatttgatcc
     2281 attccttgtg cttgtaaaac taatgggatg gagtaaagtg tatctacatc tggcgactcg
     2341 ataactgctt ctggttcgac atcacaaaat tgtgccaatt tgtttttcac gctttgagaa
     2401 acaggttgct ctgtacgtac caccaaaata ttcggttgga tacctaaacc acgtagctct
     2461 tttacactgt gctgcgttgg tttagttttc atttctccag cagctttcaa gtaaggaatc
     2521 aatgttgtat ggatatacat aacattatct gtaccgacat ctgctttcat ttgacgaagt
     2581 gcttcaagga atggcaaaga ttctatatcc cctactgttc cccctacttc tgtaatgatc
     2641 acatctgaat cagtcatttt agctgctcgc atgatttttt ctttgatctc atttgtaata
     2701 tgaggaataa cttgaacagt tgctcccaag tattcgccct tacgttcttt acgcaaaact
     2761 tcagaataga tttttcctgt cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa
     2821 cgttcgtagt ggcccagatc caaatctgtt tcagcaccat catctgttac gaatacttct
     2881 ccgtgctgat atgggctcat cgtccccgga tccacgttga tgtatggatc aaatttttgg
     2941 atagttactt ttaagccgcg gtttttcaat aagcgtccta aagaagctgc aacaatccct
     3001 tttccgattg atgaaacgac accgccggtc acaaaaatat attttgtcat aagttcaaaa
     3061 actccttttt aatttatgtg caggaaaagt ctcagacaat aaacaaagct ccctattctt
     3121 caataagaat agggagccga aatttctgta aacttctgac cctctaaaag ggtgcccaag
     3181 gtgtataata caagctgact aagggaaagt caagtactca aatgaatact atccgcttac
     3241 aattttttct ttttcgatat cctttgagaa attatttctt aagtccttta tgagtgcaaa
     3301 aaaaatttaa cccacataat aagcttgata taaacatagc taacgttaaa cctaagcaaa
     3361 tcagaaaatc gttactgaaa aattttaagt gaaataatag ataaaaaaat aaaacaaaca
     3421 ttatgataga taatatccca cctaacaaag aactactttt tcctacttgc agcaacagtt
     3481 tatatagctt atcgtagaaa caatcttttt ctttcaccct tatttacctc ctttacgtaa
     3541 aaaacgtagt ccacagttct cggatttttg gcatatcttt tctattaaat aatatcaaaa
     3601 acaaaacaat tccactaatt ttttcaaaga tgaccttttt ttcaacaaaa gtgccttgta
     3661 gggattcatt gaaggtaacg aatcacataa gtaaaaaata aggagatgcg acaaaagtcg
     3721 tgcctcctta tttctaaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     3781 tatccatcaa acatgagaag ctgtttttga aattatttct tgtgattcgg agttaaatgg
     3841 taggctcaca acctctggat aaacggt
//
LOCUS       DACCQI010000169         3864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCQI010000169 DACCQI010000000
VERSION     DACCQI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.178"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(248..469)
                     /locus_tag="IVO76_002659"
     CDS             complement(248..469)
                     /locus_tag="IVO76_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430587.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="IVO76_002660"
     CDS             complement(567..812)
                     /locus_tag="IVO76_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430588.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="IVO76_002661"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="IVO76_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..3037)
                     /locus_tag="IVO76_002662"
     CDS             complement(2036..3037)
                     /locus_tag="IVO76_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP7430589.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3196..3462)
                     /locus_tag="IVO76_002663"
     CDS             complement(3196..3462)
                     /locus_tag="IVO76_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430590.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3452..3808)
                     /locus_tag="IVO76_002664"
     CDS             complement(3452..3808)
                     /locus_tag="IVO76_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7430591.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1432 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca gttccggtaa
     2461 cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca aagcgttttg
     2521 attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg agaaaccttc
     2581 tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag tccctgtact
     2641 tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa
     2701 cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc
     2761 tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat
     2821 tttctacaat ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa
     2881 tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta
     2941 tattccatct atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg
     3001 atgctataaa tacttttatt agatttatgg ttttcattta ttgtaccttt ctctttatgt
     3061 ctctttaaag taatttttgt ataaaattta ttgactttta cactatagga caacttttat
     3121 ctatttagta caaaaaggcc cactaaacta attgattagt taggagccta ttacaggtat
     3181 attttcaact cgcttttaag caaattctac taccagcttt ttgcctaatg ccctagcgat
     3241 atcatcaagt ttatcgaaag atacgttatc ccctctctca attcgagcaa tagtagattg
     3301 agttgtatgt gctttttgtg caagctccgc ttgagttagg cctgcttcct cccgtgcatg
     3361 ataaagagca accgcagttt cgagttttct tccttcacgg tcatatgctt ctgcaaaatc
     3421 tttgtctttt tgcttttctt caatcaattt tttaatcatc atttgtgaac cactcctctc
     3481 taagttcttt tgcatgttta agttcactta ttggtgtttt ctccgttttt ttagtaaatc
     3541 catgggtaat gacatacctt ccattgacca catggaaata gatagctctt tggatgtttg
     3601 atcctacctt acttcttaat tcgtacaaat tagtgtctaa ttttttcacc catttcaacc
     3661 gttgagctac tagcatccca ttcttttctg tttcttctat tgtacgtaaa agcttcgcac
     3721 tatctttttt tggtaatgtt tccaaccatt ctaaaaattc atcatgacca ttaggtcttt
     3781 taaatgtttc aaaagatatt ttttccatac ccaattatat catatattga ttttatatta
     3841 tagtatataa cataaaaatt cgaa
//
LOCUS       DACCQI010000170         3859 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCQI010000170 DACCQI010000000
VERSION     DACCQI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.179"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            291..659
                     /locus_tag="IVO76_002665"
     CDS             291..659
                     /locus_tag="IVO76_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7430592.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            839..1780
                     /locus_tag="IVO76_002666"
     CDS             839..1780
                     /locus_tag="IVO76_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7430593.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1849..2580
                     /locus_tag="IVO76_002667"
     CDS             1849..2580
                     /locus_tag="IVO76_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7430594.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2724..>3859
                     /locus_tag="IVO76_002668"
     CDS             2724..>3859
                     /locus_tag="IVO76_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7430595.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTELIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQV"
BASE COUNT         1290 a          650 c          851 g         1068 t
ORIGIN      
        1 tgttgaatat tgtgtcttgt gaccgaggag ttgtcaggag aacaccgttt accagaggtt
       61 gtgacttgtt tttagtcacg acctcttttt ttatttattc catataaata cacattattt
      121 attccgaaaa gtgaaagcgc aaataacaaa aacaaccaac ttatttatta ttaaagaaaa
      181 gcgtatttaa gtaggacgat tctgttgaga gctttcgtag attttcccat tttcctatgg
      241 taaactgaca aagttgagaa tgttagaaca gatcgtttag gaggaaacag atgtttacga
      301 atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg ttttggagag
      361 cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata attgaagtag
      421 gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt gagtcccatt
      481 cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat atctttggac
      541 tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag cttggagaag
      601 aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca ggtaaataat
      661 agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt taattcaaat
      721 aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca aataaatcgt
      781 caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga agaaatacat
      841 ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag aggaatttgc
      901 caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc tagtagctga
      961 agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct atatgaaagc
     1021 aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa gcgaaggaca
     1081 tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa agctagaaga
     1141 ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat ttaaagaagc
     1201 tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat atgacttggg
     1261 acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc cagactggat
     1321 ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta ctggtggcat
     1381 tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg tcggacagct
     1441 gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat tatgggacgg
     1501 caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag ataaacaaat
     1561 cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt gtagcaatcc
     1621 tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt taggagcatg
     1681 tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg gaatctctcc
     1741 agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag ttttgattgt
     1801 cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat gaaatcattg
     1861 atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact tattcactat
     1921 tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg aggtcctcgt
     1981 acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc catttacctc
     2041 cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga ctggtatgcg
     2101 ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc aggggcatcg
     2161 gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc agaactgatt
     2221 aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt tgtccatgcc
     2281 ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt tatttggata
     2341 agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt tggacatacg
     2401 attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca ggatcataaa
     2461 atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt ttttgacaag
     2521 gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca tggaatctga
     2581 ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt ttatagagaa
     2641 tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa agaagcaatt
     2701 tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc attcagcttg
     2761 tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt aatttgttaa
     2821 atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact ggaagtcttg
     2881 atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac ttagaaaaac
     2941 gtaaaactga attgattcgg ctaatcgatg aacaagggaa actgactccc gagttagaag
     3001 cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt ccatacaaac
     3061 aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca ctcgcgaagt
     3121 ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat attgacaaag
     3181 aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt attgctgaac
     3241 aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat aacaaaggta
     3301 tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat gaaatgtatt
     3361 atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct actaatcgtg
     3421 gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca attttagctt
     3481 acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc gtcagagaag
     3541 cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa ctgcgcaatg
     3601 aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac ctccgtaatc
     3661 ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg gcttatcgta
     3721 ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc gaagttattt
     3781 atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct tttatccaat
     3841 taattaataa atatcaagt
//
LOCUS       DACCQI010000171         3800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCQI010000171 DACCQI010000000
VERSION     DACCQI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.180"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            304..1629
                     /locus_tag="IVO76_002669"
     CDS             304..1629
                     /locus_tag="IVO76_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7430596.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            1622..1969
                     /locus_tag="IVO76_002670"
     CDS             1622..1969
                     /locus_tag="IVO76_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430597.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            2320..3141
                     /locus_tag="IVO76_002671"
     CDS             2320..3141
                     /locus_tag="IVO76_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7430598.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            3161..3721
                     /locus_tag="IVO76_002672"
     CDS             3161..3721
                     /locus_tag="IVO76_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP7430599.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT         1449 a          495 c          704 g         1152 t
ORIGIN      
        1 gaccagacgg aaggacgaat gttgttgagg ttttagaacc atataaaaca gcatagttct
       61 caaattgttt aaaatgattg tcaagttaaa aaaatctggg atcatataaa atagtatagt
      121 tctcaaatat gattagaaac cctattaaag agcaatatta agccaactta cttaaatcgt
      181 ttaattaaga acaataatct cttttagtca gacttttagt ctgacttttt tgtatttcca
      241 aaatttattc tttacaaaaa cgaacatacg tttgtacaat tacttgtgaa tgggggaata
      301 aaaatgtttg attacagtag agaaccggtg aatgactatt ttttaatcga catgaagtct
      361 ttctatgcta gcgtggaatg tattgaacga aatttagacc caatgactac accgttaatt
      421 gtcatgagta gatcggacaa cactggttca ggtctgactc tagcttcttc acctactgca
      481 aagaaaaaat atcatattac taatgttagt agagctactg atttacctta tccattacca
      541 aaagaacttc atattgttcc accacgaatg aacttatata taaaaaaaaa tatggaagtt
      601 aatcagattt ttagaaagta tgtcgcagat gaagatttat tgatttattc gattgatgaa
      661 tcaattttaa aagtgacaaa atccttaaat ttatttacta gttcaggaac aagaagcgag
      721 agaagaagag aattagctaa aataatcaaa gatgagattc agaaagaaac aggtctaata
      781 gcaacagtcg gaataggaga taacccatta ttagctaaac ttgcactgga caatgaagcg
      841 aagagtagta aagaatttat agctgagtgg acttatgaga ctgtacctga aaaaatatgg
      901 aaaataaacg agatgactga cttttggggc attggatcac gcatgaaaaa aagattgaat
      961 catatgggaa ttatgagtat agaagattta gcaaattgga atccctacac aattaaaagt
     1021 cagttaggtg taatcggact ccagttatat ttccatacca atggaattga tcgtacggat
     1081 attgctattc caccagaacc aaccaaagaa aagtcttatg ggaattcaca agttctacca
     1141 aaagattata cgaggagaga agaagtagaa ctagtagtca aggagatggc agagcaagta
     1201 gccattcgaa ttagacaaca caattgcaaa actggttgtg tacgcttagc aattggtacg
     1261 tcaattttgg aagaaaaatc aggtttctcg caccagatga aaataccaat tacagataat
     1321 acgaaagaat tacaaaatta ttgtcttttt ttattcaata agttttatga ggggcaggaa
     1381 gtacgacata taggagtaac ttatagcaga ttagtataca cagattccct acaattagat
     1441 ttatttagtg atccaagaaa aaagattgct gaagaaaatt tggataggat tattgataag
     1501 attagacaaa aatatggatt tacagctatt atacatgctt catctatgtt agaaggtgca
     1561 agaagtatta cgagaagcac tttaatagga ggacacgcag gaggattagg aggtttgcat
     1621 aatgattgaa tataacgata gaggacgttt aaaatggtcg ggattttatt tgtctgatca
     1681 tactgaaaga atcaatgcag acaatgtaca acgtgctaat cataatttgg caaaagaaca
     1741 aatgacgact gaagagattg cgaaaatact gagaactgct gtcataaaaa atagacccgt
     1801 ctgcattcaa aaagaggaac gaaacgctga aggttactat ccagatgatg tagtaggaaa
     1861 aattattgga tatgacgagc ttggtgtatt tgtggaagaa gaaaaagttc actatgatga
     1921 aattaggaat gtttctttct cggaattatt aaaatggagt cagatatgaa cattattaaa
     1981 aaatacgaat tcggttatat tacgtatgaa cagctaatag aggaattatg gggatatggt
     2041 caacaattaa ttaatgaagt ggggattgat tgcttttgtt tctatattga atccggagca
     2101 ggataccata agaacagata ctatatattt ccatatccac caaaatagtc tcttagatat
     2161 ttttaaattt tctccaaata ctttcaatta ttgtgaaata tttcccttta attaatcaaa
     2221 ttttttataa aagtaatata taaaataaat tgcttgaagt attatattta gatatcataa
     2281 tttatggaag aaaaattaat taaaaaggat gagattataa tggggttact ggttccgaga
     2341 gctaatactt atactatacc tttaacgaaa cgccaattaa ttgctaaaag atatcaaagg
     2401 attactagag ccatcaatag agagttttgg aattctgaaa gtgatactgc tcacagttta
     2461 tacgtaggtt cttatgggag aggaacggca attagtacta gtgatataga tataatagtt
     2521 gaattaccga tggcagaatt tgacagattc aaaaattatt tatcaaatgg cccgtctaag
     2581 ttattacaag ttataaaaaa cgcatttcag gaaatactac caaatagtga tattagagct
     2641 gatggacaag ttgttaaaat aaatttccat gatggaatca aatttgaaat agttccagct
     2701 tttaatgaaa aagattattg gggtgagtca aaagggttta tttatccaga ttcaaacatg
     2761 ggagggaact ggaaagctac aaatcccaaa aaagagcaag aagccatgaa attaaagaat
     2821 acaaaaagta ataatttatt atatgctacg tgtaaacatt ttcgacatgt acgagataca
     2881 gaatttacga gttatcatct ttcaggaata gtaattgaca gtttcgtata tgaggctatg
     2941 ggaaattgga aattcgttga aaataacagt gggggtcaaa acattagttc tgtatcttat
     3001 gagacagctt tattagaata ctataactca cataaagtta tgggcggttt aaatttatac
     3061 tctcctggaa gcaatcaatt tgttaattct gatagtagta tcatatgttt agaaaaagta
     3121 ttaaaaaaaa tagctttata aagttttatg gaggaacaac atgggagacg aaaaaaagcg
     3181 cttggaagtt caattacgtg aagcatatgg acgagtaacg tatacttaca ccactcattt
     3241 aaagcaagcc gataatatta tttctgacaa taaaatttta aggtgtcttc aaattggttt
     3301 gtcagccctt actactggag gggtacttag taccattatt actgataata gtgagttaac
     3361 ctttgttgct ggattatgtt caacaatttc tcttggatta aacttatact ttaagaattt
     3421 tgatcttgct gaagaagcta aaaatcatca aattgttgcc gataaattat ggttgatcag
     3481 agaagaatac gtaagcttac taacagattt aaatcgttta agtgttgaag atattgtgaa
     3541 aaaaagagat caattaatga acgaaactca tgaagcatat aaaaatgccc ctaaaacaaa
     3601 tagtaaaagc tatgaatcag ctcagaaagc attaaaatct gaagaagagc aattttttac
     3661 agaagaggaa ttggataaga tgttaccaga gcatcttaga aaaaagagtt ctaaatattg
     3721 aactcttttt ttatgcttaa aattctcttg taatttttct attcatctat atttaagaag
     3781 aaatatgatg atagaaacat
//
LOCUS       DACCQI010000172         3791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCQI010000172 DACCQI010000000
VERSION     DACCQI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.181"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IVO76_002673"
     CDS             23..646
                     /locus_tag="IVO76_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430600.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IVO76_002674"
     CDS             1096..2145
                     /locus_tag="IVO76_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7430601.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IVO76_002675"
     CDS             2142..3023
                     /locus_tag="IVO76_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7430602.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..>3791
                     /locus_tag="IVO76_002676"
     CDS             3026..>3791
                     /locus_tag="IVO76_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7430603.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVV"
BASE COUNT         1265 a          658 c          826 g         1042 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg g
//
LOCUS       DACCQI010000173         3763 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCQI010000173 DACCQI010000000
VERSION     DACCQI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.182"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(7..963)
                     /locus_tag="IVO76_002677"
     CDS             complement(7..963)
                     /locus_tag="IVO76_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7430604.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1137..1697
                     /locus_tag="IVO76_002678"
     CDS             1137..1697
                     /locus_tag="IVO76_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430605.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1690..2514
                     /locus_tag="IVO76_002679"
     CDS             1690..2514
                     /locus_tag="IVO76_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7430606.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            2524..3141
                     /locus_tag="IVO76_002680"
     CDS             2524..3141
                     /locus_tag="IVO76_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7430607.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3159..>3763
                     /locus_tag="IVO76_002681"
     CDS             3159..>3763
                     /locus_tag="IVO76_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7430608.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAIT"
BASE COUNT         1228 a          692 c          822 g         1021 t
ORIGIN      
        1 ttaaaattac atatttttcc atagaacttg catttgatcc aagatttcga tgacatcttt
       61 tgtcaagaag agagagcgat tgggcaagtc accttcaatc atgtgtacca tattctttat
      121 ttcgtaattc attgcttcag atgtattccc gctttcgatg aattcctttg taccatctgt
      181 aaataagatc tccgcacgat cagcacgcgg gtaatcagca atcgtcacat aaccgttctc
      241 aaaggcaacg ataccgactt ttggcatttt tgcctgaaaa gtcaagctga cagcagcaag
      301 ttcattttct ttgttacgca agatagtgac ggattgttcg tccacgcctg tttcaaaagg
      361 aaccattgta gaggcaacta cttcaggttg agagctcaaa aagaaacgag cgaaagatac
      421 agcatacgta ccgatatcca acagagctcc tccagctaat tctggattga agaaccggtt
      481 tttagggtct ggttctttgt aactgccgaa aggtgcttgg atcattttta aagcccccag
      541 ttttcctgta tccattattg agcgtaactg ctgataaagt ggcatgttga agattgtcat
      601 cgcttctgct aagatcaaat tcttttcttc tgctaagcgc atagcgtcag ccagttcttt
      661 tttattcatc gtgatggctt tttcacatag aacgtgtttg cctgcttcca atgctgccaa
      721 gatatggtcg atatgctgtc tatttggtac agcaatgtac acgatgtcga tttcagcatc
      781 gtctaacatt tcttgatagc ttccgtaagc ttttggaata ttgtaacggt gagcgaagtt
      841 ttccgctttt tctgaagttc tggaagcaac tgctgttagt tcgctcgtct cttgattgaa
      901 gctttcggca aactcatgtg cgatggtacc aagaccaacg attccccatt gatatttttt
      961 catctgaaaa cgctccttca tagttggatt gagtttcttg tctgtctatt attataacaa
     1021 aaatcacgaa gaataaagag gagaggactt gaaacattaa aaaaaatgaa agcagcgatt
     1081 taagaggtgc tttttgatca aaacttaagt agaatagtta aaaggagggg ataaacgtgc
     1141 atcaagtata tattgatctt attatgagtg tattattaga acagtttgaa actgaaaaaa
     1201 attttttaga tgaatacttg ctgatcagtc cagaacaatg gcaaaaatgg aaagatggcg
     1261 agaaaaatct gccatctgaa gcgatgcaga aagtaaaaaa tcttttcact gattatgaat
     1321 ggatgctaac gcaaaagatt ttgcgtcaaa cgattatttt tcccgaaaaa cgaaacgttg
     1381 ccgttgcaga gtacaagcaa atcaaaacac gcatcgcgca aaaatggctt agcagcaaag
     1441 cagcaaaagt cgagctgatc cctttgaaaa ataatcagca ggctggtaga taccttgact
     1501 tgaaagtaag tattcaatat gatgaatggg gctatgatga tattttgaat tttcgtctgc
     1561 cagcagatgt tcaaaaacag atcgaaggag aaaaaatcga acttctcgat tgggtgaatg
     1621 aaaatctgga agaaacttac gttcatgagg cgaatgggga acgtgaagaa caacgaaagg
     1681 atttttcaag tgaataaatt tttaggattt ggagcagttg tgattatgat cgcagcaatg
     1741 ggctatgtta tttttagcga tgatgattcg catgttcaag ccggcaatca agcagtcgaa
     1801 cgaacaagca ccaaaacaac tgctagtaaa gaaaagaaca gtcaaacaac cagcagcaag
     1861 gtaaaggaca aagaaacaaa agatcgtacg tccaaacaat tgccagatgt taaaacagac
     1921 gattggaagc tagttttggt tggtccagat cacaaattag aaaaagaaat tgatgagtct
     1981 actcaattgg catcattaag taacggatac ttgatcgaca aacgcattgc tccagaatat
     2041 gaagcatttg ccaaggctgc tgaagcagct ggatttccat tagtgatggt ctcagcttac
     2101 cggtctgtat catcccaaca acaagttttc gaacaaaatg tccaagatgt gatgagtcgt
     2161 caaggtgtat cagaagagga ggcgacaaag attactaaag aaacgattac ggagccagga
     2221 tacagcgaac accacacagg tctggcagtc gatgtcgtcg atcaaaactg gtacaacagt
     2281 tatccttcta ctgtattgga tgcaagttac ggagatcagc ctggagctaa atggatcgca
     2341 gataatgctg cgcagtatgg atttatcgtt cgttatccaa aaggacacga agatataact
     2401 aagatcacat atgaaccttg gcacttacgt tatgtcggta aagaaaacgc tgagtatatc
     2461 accaaacacc aattgacgct agaagaatat ctgaatgaat taaatgaaaa gtaggaattg
     2521 atcatggcga aaaaaaagtg gaaaaagcaa aagcgcaaga atatccgttg gccagcagtg
     2581 gttgccggtg tagcagtgat actcttagcc tttgtttttt ccttgaagaa tctgtcttct
     2641 ccttttactg ataatcagca agaaaatgaa gcgcaatccc atcagcaatt tattgaacgg
     2701 cttgttccgc atgcccaaga actacaagat ggttatggag tgcttcctag tatcatttta
     2761 ggacaagcta tattagaatc aaattgggga aaaagtacct tggctagcaa atacaataat
     2821 cttttcggga tcaaggctta tggggatcag aagaaagtaa gcctggaaac aaaggaattt
     2881 gttaacgaag aatggataac tatccaagga gacttcaaag tgtatgattc atgggaacag
     2941 tccatggatg accatacaca actgttcgtc caaggagtag attggaatcc tgcgctgtat
     3001 gaaaaagtga ttacggccac caactatcag gaagccgcac aagcattgca agatgcaggt
     3061 tatgcgacag accccggtta tgcgcaaaaa atcatccagg tcatcgagac gtatcaattg
     3121 aatcagtatg accggagata gcaaaggaga agtggctgat ggcagaacaa tttacgccag
     3181 aaatcatgac ggaattgaga aaagatatcg taagagtacc ggaaatcatt ttagaagcaa
     3241 gcgggatcaa gatatttgga aaattgatcc gttcgatcat ctttactacg gatattgcga
     3301 tcatacgtaa tacaaatgcg aatgcagtaa ttgctgtgta tccttttaca cctcatccag
     3361 cgattactaa aagcattatt gaggcagctg acattccagt tttctccggt gtaggtggtg
     3421 gtttgacaca aggtgctcgt tcttcctaca tgagcatgtt cgcagaagcg caaggttcgt
     3481 taggggtcgt tttgaacggt cctacgcctt tagagacagt aaaagaagtt tgtcgagtag
     3541 ttgatattcc agttatcagt acggtaacgt cgaaatatac agaaatagat gaaaagttag
     3601 cagctggtat ttcgattatc aatatcagtg caggtaaaga aactgcagaa gcagtacgcc
     3661 attttcgtaa aaaatatcct gaacttccta tcattgcgac aggtggtcca acggaagaaa
     3721 gtattgctga gaccattcaa gcaggagcaa atgccatcac ata
//
LOCUS       DACCQI010000174         3720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCQI010000174 DACCQI010000000
VERSION     DACCQI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.183"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            484..780
                     /locus_tag="IVO76_002682"
     CDS             484..780
                     /locus_tag="IVO76_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAP7430609.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            781..1197
                     /locus_tag="IVO76_002683"
     CDS             781..1197
                     /locus_tag="IVO76_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAP7430610.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            1217..2761
                     /locus_tag="IVO76_002684"
     CDS             1217..2761
                     /locus_tag="IVO76_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7430611.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            2904..3128
                     /locus_tag="IVO76_002685"
     CDS             2904..3128
                     /locus_tag="IVO76_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430612.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            3143..>3720
                     /locus_tag="IVO76_002686"
     CDS             3143..>3720
                     /locus_tag="IVO76_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7430613.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDK"
BASE COUNT         1318 a          620 c          807 g          975 t
ORIGIN      
        1 ctttcctaac attgatttaa tagtctttaa agtattctgc ttcatcaccc attaggtgat
       61 gaaaaaagaa cccctttctg atttagcgga caaatacttt gacctaatct cagaagatat
      121 aaaagaaaaa ataataaaat atccgattta tactttgtat aaagaactaa agtagttagc
      181 aaatcattta cagcaaatca ctatgttcca cataccattt tacatactta ccttatccac
      241 gacacatgtt gagatagtat cgaagataaa gaaagttgct ccatgttgtt tttgcgagat
      301 ggggaaatca aagccacatt gacaaccatg atgaataagc ttgctttcag tcataagcta
      361 atcctagaac cacttttcaa gtcagttagc caaatcgatg aagaaagcga ccgtgaaaga
      421 atggacgcta ttgataagct aatggagcaa ctattggaag aacgcaatac ccttattgca
      481 ctgatgtcca aaggtttcct agaacctgct ctttttaatc aggaacgaaa tgttttagat
      541 agtgagatta aaaatcttac taccgaaaaa acaaacctgg taacgaactc tgcgagtggg
      601 gttttgcgag caaacgagat aaaggacctc attaattatg tgtcagcaga taattttaat
      661 ggtgactaca cggaagaact atttgaagaa tttgtagtga acatcattgt aaattccagg
      721 gatgagctga cattcaattt gaaatgcggt ctttccctga aagaaaaggt ggtgagataa
      781 atggcatata ttccatatgg atacaaaatt caagatggag tggttactgt cgatgaaaag
      841 gcagcaggtc aagtaaaggt attctttgag aaatacatat caggactatc ccttacagtg
      901 gctggcgaac aggcaggtat tgaaaagaca cattctgtga tggggcgcat tttgaaaaac
      961 gtcaactacc ttggaaatga tacgtatcca gcaattattg ataaagagat atttgataaa
     1021 gctgaagaag ttagagataa acgtgcaaag gatttaggac gagtggtaga gcttgccgct
     1081 ttcacctctc cccctcccaa agaacgattc aaaatgaaaa aggcagataa taagatgcca
     1141 gtggatcctt ttgaacgagc agaatactta tacagtctga tagaaagcga ggaataaagt
     1201 gacagagaaa aatataatgg ttattcctgc tcgtaaaaga gtaggaagta cagccgcaaa
     1261 agaaaagata aagaaacttc gtgttgctgc ctattgccgt gtttctacag aaacagaaga
     1321 acaaaattct agctatgagg ttcaggtctc acactataca gagtttataa agaaaaataa
     1381 tgaatgggag tttgctggca tctttgcaga tgatggtatc tctggtacga acactaaaaa
     1441 gcgtgacgaa tttaatcgca tgattgcaga gtgtatggac ggtaacatcg acatggttat
     1501 tacaaaatcc atcagccgat ttgcgcgtaa caccctagac tgccttcaat acattagaca
     1561 gctcaaggat aagaacatat cagtttattt tgagaaagag aacatcaaca ccacggatgc
     1621 caaaggtgag gttttgctga ctattatggc atctttggca cagcaggaaa gccagagcct
     1681 ttcacaaaac gttaagcttg ggctacagta ccgataccaa caaggaaagg tgcaggtcaa
     1741 ccataagcga tttatgggct acacgaaaga tgaagatgga aatttaacta ttgtccccga
     1801 agaagctgag attatcaaac gcatctaccg agaatacctt gagggtcaga gtctagtggg
     1861 cattggtcga gcccttgaaa aggatggtat tttaacagca gcgggaaaac caagatggcg
     1921 accagaatca gttaagaaga tacttcaaaa cgaaaaatac atcggagatg cccttctgca
     1981 aaagactgtc acagtagatt ttctgaccaa gaaacgagtg aaaaatgaag ggcatgttcc
     2041 ccaatattat gttgaaaata gccatgaagc gattattcct aaagagttat ttttgcaggc
     2101 gcaagaagaa attcatcgga gaagcaatat ctacacagga gaaggcaaga acaaaagaat
     2161 ttacagtagc aagtacgctt taagtgccat caccttctgt ggagattgtg gcgatattta
     2221 tagacgggtc tattggaata tccacggaag aaaagaattt gtctggcgat gcgtgactag
     2281 aatcgagcaa ggtcctgaag tttgtaagaa ccgaaccgta aaagaagatg aactttatgg
     2341 tgcagtaatg actgcgatta ataagctact tgcaggtggg aacaatatga taaagacact
     2401 ggaagagaat attcatgctg tgattggcga aaccacagaa taccaaattt cagagattaa
     2461 caactcacta gaggaaaaac aaaaagaact aatcaagctg gcgaacaagg gtcaagacta
     2521 tgaccactta gcagatgaga ttgatgagct gagagacaag cgacagatcc ttttagtaga
     2581 agatgcctcc ctcagtggcg agaacgagcg aatcaatgag ctgattgaat ttatccgcaa
     2641 gaacaaattc cgcaccttag aatatgatga taagcttgta aggaagataa tccagagcgt
     2701 tacagtctat gaaggccact tcgtcatagc cttcaaatct ggcatcgaaa tggaaatatg
     2761 aaaaccagaa gtaaatagcc catgactcta cagtagagtt gtgggttttc ttttgcttgc
     2821 tgattataat aatattttaa taactattgt gtttatcaac caaatggttt ataatattct
     2881 tagcgtaaac ttggaggtgc tgtatgacta cggcagaaat gattaaagaa ctgtgtgagc
     2941 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
     3001 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
     3061 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
     3121 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
     3181 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
     3241 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
     3301 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
     3361 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
     3421 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
     3481 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
     3541 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
     3601 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
     3661 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
//
LOCUS       DACCQI010000175         3458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCQI010000175 DACCQI010000000
VERSION     DACCQI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.184"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..375)
                     /locus_tag="IVO76_002687"
     CDS             complement(<1..375)
                     /locus_tag="IVO76_002687"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7430614.1"
                     /translation="MKNISLELKNIEVNFGNKELVAIKYLTVYENDRIGIVGRNGQGK
                     TTLLNLIQGKLSPNSGEVNRFVEFNYFKQMEETNNLYEFDDLNAELISRFTIPRNTIE
                     VLSGGEENKLRLARVLSNYKMGL"
     gene            complement(984..1703)
                     /gene="catA"
                     /locus_tag="IVO76_002688"
                     /pseudo
     CDS             complement(984..1703)
                     /gene="catA"
                     /locus_tag="IVO76_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
     gene            1912..2871
                     /locus_tag="IVO76_002689"
     CDS             1912..2871
                     /locus_tag="IVO76_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7430615.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKVAKIAEFLKRSRTPIYNVIHF
                     LKEGHTALDYYQQYKENKKRCGRQKIILPKEQQTYIKEKVNQGWTPDVIIGRKEMIIS
                     CSMRTLYRQFKEKVFDEATLPMKGKRKPNGHQERRGKQAFKRNIVERKTDYPDFEEEF
                     GHIEGDTIVGAHHKSAIITLVERLSKVIITLKPDGRRASDIEATMDHWFNAIPRHLFK
                     SITFDCGKEFSNWKALSNQHDVAIYFADPGTPSQRGLNEHSNGLLRKDGLPKAMDFNL
                     VDQSFISSVADKRNNIPRKILNYQTPLEVFMSYMNKDILSSLI"
     gene            3189..>3458
                     /locus_tag="IVO76_002690"
     CDS             3189..>3458
                     /locus_tag="IVO76_002690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430616.1"
                     /translation="MKNKLVSYAIMTVNNRYNRDYYSLFIPFVAETIRLKEFKYITPQ
                     IIKKEIQEKYDLEIPTAVINQIFNKLDKEKLIERKYYADQKKYLYH"
BASE COUNT         1241 a          570 c          536 g         1111 t
ORIGIN      
        1 taatcccatc ttataatttg atagcactct agctaaacgt aatttatttt cttcacctcc
       61 gcttagtact tcgatcgtat ttctcggaat agtgaatcga ctaataagtt cagcgttaag
      121 atcatcaaat tcgtataaat tattggtttc ttccatctgt ttaaagtaat taaactccac
      181 aaatcggtta acttcccctg aatttggtga taactttcct tggataagat tcagtagcgt
      241 tgttttccct tgtccgtttc ttccgacaat gccaattcga tcattctcat atacagttaa
      301 atatttaatg gctactagtt ctttgtttcc aaaatttact tctatatttt ttaattctaa
      361 agatatattt ttcattattt tgcactcctt aaattaggcc gtgcaatcaa cagatttttg
      421 ggtacacaaa aaagagatag atttctacct cttttgacaa tttccatatc tatattttct
      481 tagataaata gaatttgaaa aatagacaga ttgctcccct gtttctgcca atataacagg
      541 accttttgaa taatagtatt taattattat ttcaaaaggt taaaccacaa tcttatagat
      601 gctgtcattt tccaaattct cctttgtaca tcctatttgt atactagata ctgctcaaat
      661 gaacacgtat ctttctacca acgtctcata aagcttaaaa catttttttc aaatagtcaa
      721 gttatgctca cctattatag gtcaagataa cttataatag gtgatatgaa aaaaaatact
      781 cttgctcctt tttcgccatt caaggtaact tcaatataac atgaaattca catggcgttt
      841 tttagttagc ggtggctaac ttcattatat aatccaccgt aaaaaatatt attagttaca
      901 tttgtcatta tagtcttaac ttaagagttg tggcattgct tattacatat gtaataagca
      961 atgcgttttg tgaagtagta cacttaaatt aacgggcagg ttagtgacat tagaaaactg
     1021 actgtaaaaa gtacagtcgg cattgtctca tattataaaa gccagtcatt aggcctatct
     1081 gacaattcct gaatagagtt cataaacaat cctgcatgat aaccatcaca aacagaatga
     1141 tgtacctgta aagatagcgg taaatatatt gaattacctt tattaatgaa ttttcctgct
     1201 gtaataatgg gtagaaggta attactatta ttattgacat ttaagttaaa cccagtaaat
     1261 gaagtccatg gaataataga aagagaaaaa gcattttcag gtataggtgt tttgggaaac
     1321 aatttccccg aaccattata tttctctaca tcagaaaggt ataaatcata aaactctttg
     1381 aagtcattct ttacaggagt ccaaatacca gagaatgttt tagatacact atcaaaaatt
     1441 gtataaagtg gctctaactt atcccaataa cctaactctc cgtcgctatt gtaaccagtt
     1501 ctaaaagctg tatttgagtt tatcaccctt gtcactaaga aaataaatgc agggtaaaat
     1561 ttatatcctt cttgttttat gtttcggtat aaaacactaa tatcaatttc tgtggttata
     1621 ctaaaagtcg tttgttggtt caaataatga ttaaatatct cttttctctt ccaattgtct
     1681 aaatcaattt tattaaagtt catttgatat gcctcctaaa tttttatcta aagtgaattt
     1741 aggaggctga cttgtctgct ttcttcatta gaatcaatcc ttttttaaaa gtcagtatta
     1801 ttgtaacata aatatacgcc gattgtaaaa ttaagctaga caaataaaaa agccattcgt
     1861 ggtacactca aaatgtattt ccgaccaaag aaaacatagg agtgatcacg aatgacctac
     1921 acccatctta ccacggatga attagtgatg atagagtctt attatcacca aaatattaaa
     1981 gtcgctaaaa ttgctgagtt cttgaaacga tctcgaacac ccatttataa tgtcattcat
     2041 ttcttgaaag aaggccatac ggccctcgat tactaccaac aatataaaga aaataagaaa
     2101 cggtgtggca gacagaaaat catcttacca aaagaacaac agacctatat caaggaaaaa
     2161 gtaaatcaag gctggactcc tgatgtcatt attggacgaa aagaaatgat catcagctgt
     2221 tctatgcgga ctctttaccg ccagtttaaa gaaaaagtct tcgatgaagc tacacttcct
     2281 atgaaaggga aaagaaaacc caatggtcat caagaacgca gaggaaaaca agccttcaaa
     2341 cggaatattg ttgaaagaaa gaccgattat cctgacttcg aagaagagtt cggtcatatc
     2401 gaaggcgaca ctattgtggg ggctcatcac aagagcgcca tcattacctt ggtagaacgt
     2461 ctttctaagg ttattattac tttgaagccc gatgggcgga gagccagcga tattgaagct
     2521 accatggatc attggtttaa tgccattcca aggcatttat ttaaatcgat cacttttgac
     2581 tgcggaaaag aattctctaa ctggaaagct ttaagtaacc aacacgacgt tgccatctat
     2641 tttgctgacc caggaacacc ttcgcaacgt ggtctgaatg aacattccaa cggtctcttg
     2701 cgaaaagacg gattgcctaa ggcgatggat ttcaatctgg tggaccaaag tttcatttct
     2761 tctgttgcag acaaacgaaa caacatccct agaaaaatat tgaattacca aacgcccttg
     2821 gaagtattta tgagttacat gaataaagat attttgtcta gcttaatttg acaaatcaaa
     2881 aaattaaaaa agggataaaa aatatgaaga tcgaatgact tatcatgtaa tcatgaataa
     2941 tttgaagtaa gaggagctaa tcaagaagat atctgaagaa aaagatgtgg caagagatac
     3001 tgtgtataag attaagaaat aatttgagat aaaggaataa aaagaaatta ttacttttat
     3061 tgtaaccaag gaataaatta aataattaaa tttataatat taaaataact atcgtataga
     3121 attcataagg aataatcact ataatatgag taagagtatt ataatcattt tatggaggga
     3181 ttgtcttttt gaagaataaa ttggtttcat atgcaataat gactgtaaat aatcgatata
     3241 acagagatta ttattctctt tttataccat ttgttgcaga aacaattaga ttaaaagaat
     3301 ttaaatacat aacaccgcag ataataaaaa aagaaattca agaaaaatat gacttagaaa
     3361 ttccaactgc tgtaataaat caaatattta ataaactcga caaagaaaaa cttattgaaa
     3421 gaaaatatta tgcggatcag aagaaatact tatatcat
//
LOCUS       DACCQI010000176         3453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCQI010000176 DACCQI010000000
VERSION     DACCQI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.185"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..804
                     /locus_tag="IVO76_002691"
     CDS             <1..804
                     /locus_tag="IVO76_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP7430617.1"
                     /translation="YYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQ
                     LSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLT
                     YVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRG
                     KEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRL
                     ELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
     gene            complement(934..1383)
                     /locus_tag="IVO76_002692"
                     /pseudo
     CDS             complement(934..1383)
                     /locus_tag="IVO76_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1727..2134
                     /locus_tag="IVO76_002693"
     CDS             1727..2134
                     /locus_tag="IVO76_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430618.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            2148..2549
                     /locus_tag="IVO76_002694"
     CDS             2148..2549
                     /locus_tag="IVO76_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430619.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            2551..2922
                     /locus_tag="IVO76_002695"
     CDS             2551..2922
                     /locus_tag="IVO76_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430620.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(2958..3260)
                     /locus_tag="IVO76_002696"
     CDS             complement(2958..3260)
                     /locus_tag="IVO76_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430621.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         1191 a          548 c          617 g         1097 t
ORIGIN      
        1 tactattatc aagcgaaacc gatcgaaaat gagtccgact tagaagagat cgttcaggaa
       61 gagtttattc gaaaccgaaa ggcttacggt acccgaaaat tgaagaagtg tttagcaaag
      121 cgtgggcttc aactcagtcg gcgccgaatc ggtcgaatca tgaaacgccg cggattgaca
      181 tctacctata cgatcgctca ttttaaaggg caacgaacag cttgtaatga agcgaaaaca
      241 gcgaatgtat tagatcggac ctttacacaa gaacagccat tggaagccat cgttacggat
      301 cttacttatg ttcgcgtggg gaaaaagtgg cattatatct gcttaatact tgatttgttt
      361 aatcgagaaa ttattggtta ttcctgtggt gagaagaaag atgcctcatt ggtaaaagaa
      421 gcctttggac ggataccgta ttctttaaca gacgtcaagc tttttcatac agaccgggga
      481 aaggaatttg ataaccaaac cattcatgag attctgaatg gttttggaat tactcgttca
      541 ttgagtagga agggttgtcc gtatgataat gccgttgtgg aatcaaccta taaatctgtc
      601 aaagtagaat tcgtgcatca ataccaattt gagacactgg cacagctacg tctagaattg
      661 tttgattatg tgcattggtg gaactatcta cgcttacatg gcacgttggc gtatgaaaca
      721 ccgatccaaa ttcgacaaca gagattggcg aagcgaatcc ttgataatga gcgcggatct
      781 gatacctctg gagaggcagc gtaattgaat gattgtgctt ctgccggaga aaatcagatc
      841 cgaggatgct cattgtcaag ggcaatcgta gccataacac cgcagcattc acaacacctt
      901 ataatttttg tcaaaaaaag tgttgccatt ccactttttt aaatatgcgt tctctgctct
      961 taaacgaata acttcttctt caagagactc atctttaagt tttttaggaa tgtttagctt
     1021 ggaattcata ctaatttttc tgcccctttt ttggctctca agtgaagaag caccgccttc
     1081 ttcatattgc tctatccatt tacttagagt tctatttgaa ccgatattaa attttttagc
     1141 agtttcttgg atagaaagac catttgtttc catatattct ataacatcaa gtttaaattt
     1201 tgtagtgtag cttttgccac ctccaaccaa gccttcccaa ccatgatagt tataaatcct
     1261 tacccaatgt ctaaccagtg tacgatttat ttgatattta tgtgcaagat atttgtagcc
     1321 gccttcgtta tttaaatagt ctgaaactac ttttttctta aaaacaaatg tatatttccg
     1381 caaaaaaagc acccctttta attagatttc tagtctaact tttggggtgc acatcatttt
     1441 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
     1501 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
     1561 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
     1621 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
     1681 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
     1741 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
     1801 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
     1861 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
     1921 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
     1981 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
     2041 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
     2101 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
     2161 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
     2221 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
     2281 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
     2341 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
     2401 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     2461 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     2521 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     2581 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     2641 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     2701 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     2761 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     2821 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     2881 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     2941 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     3001 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     3061 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     3121 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     3181 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     3241 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     3301 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     3361 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     3421 tttcctgcaa taacaaacac aacagaagac gtg
//
LOCUS       DACCQI010000177         3415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCQI010000177 DACCQI010000000
VERSION     DACCQI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.186"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            238..1629
                     /locus_tag="IVO76_002697"
     CDS             238..1629
                     /locus_tag="IVO76_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7430622.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1667..2497
                     /locus_tag="IVO76_002698"
     CDS             1667..2497
                     /locus_tag="IVO76_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7430623.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2469..>3415
                     /locus_tag="IVO76_002699"
     CDS             2469..>3415
                     /locus_tag="IVO76_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7430624.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINP"
BASE COUNT         1214 a          473 c          650 g         1078 t
ORIGIN      
        1 tatatgaata ttgtaacttg tgaataagga attagcagga gaatactagt tcagcataaa
       61 ggaagtgatg ggacttctga gacaactttc tgttatgcag aaatacacat ataaacagac
      121 aataaacgct tttccccgtt tcttttaatt tcctttacaa tttggttatt cactaggttt
      181 ttaaactttg ctatggtaag atgatagagg cattatttta tggttaggag tgtatatatg
      241 aataaaaatg gggagtggaa tgcggcacgc agaatattca ttattcttat ggatgtactt
      301 gtattaaatg tatcgatcta tcttagtttt ttgctgaaat tccagggaga agtaccagtg
      361 agaaacttag aaacctttca gcattcagca atttttattt ccatcatctt tattgcgttg
      421 aatattttgt taggagctta tgtattctat aaccgaatga tcagtgatat tgtcttcatt
      481 actgttattg gacaagtact aatagcattg gggatcatgg tagttacttt tgctggccga
      541 tggtttgcat ttccgcgtgg cgtcattttg atctcgttta tcctaggtac gatattactt
      601 agtgtttacc ggatagtcgt ttacaaactg tacttaagag tcagtcgtga taagaaagtc
      661 cttatcgtgg gtttggagaa agatgtcgca ccagctatcc aaaattttca atctaagaaa
      721 aacaataaac ataaggttga agcagtggca atcgataatt attacgaaaa tacaaaaaaa
      781 ttgatcgatg atatggatat cgtctatttg gcttctaaaa tcccagaaga tgaaaaaatc
      841 aacatttatc aattagtgac taaaaaagag aagaaattat ttttaaatac gacttttgag
      901 aatctgacaa tgataaatcc gaatattatg aattttgaag atgaaagcat cattgaagct
      961 tctgggttca gaatccaacc agaatacgaa ctattcaagc gtctctttga tatcatcgtt
     1021 tcgcttattt tactgatcct cgcctcacct tttatgctaa tcacagcgat tttggtaaaa
     1081 acaacttcac ctggaccaat catctataaa caagtacgta ttacgaaaaa tcaaaaggaa
     1141 ttttcaatct ataaattccg ttccatgagt gcgacagctg aagcaaaatc aggaccagtc
     1201 ttggctaaaa gtaacgatgc acgtgtgaca ccagtaggta aatttatccg tgctgtacgt
     1261 tttgacgaat tacctcaaat ctttaatgta ttgaaaggcg atatgtcgat tgtcggtcca
     1321 aggccagaaa gaccattttt cgttgatcag ttcaatgaag aaaatcctta ttattactta
     1381 agacacaatg tacgtgcggg aattaccgga tatgcacaag tatacggaaa atatgtatca
     1441 gattataaca gtaaactacg ttttgatttg ttgtatatca aaaagtattc cttactatta
     1501 gatttgaaga ttttacttca aacaatcaaa atcttatttg ataagatgtc ctcacaagga
     1561 ctagatgaag atgaaaatat cagttcatta gcagaaagtg catttccaaa agaaaaaacg
     1621 tatcgttaga ttggaaaaat tttgagcaaa gaagtaggag agaacattgg aaaataaaaa
     1681 tataagtata ctgctttctt tgtatgataa agaaaagccg gaatatttaa aggaagcatt
     1741 agctagtatc tttgagcaaa cggtgatgcc ggcagaaatc gtattggttt atgatggccc
     1801 tataaatgag aagcttcagg cagttgtcac ttatttccag caaaaagttc ctcacttgtt
     1861 tacagttgta aaattagaaa aaaatcaagg attggggatt gctttagcaa ctggattaga
     1921 acatactaaa aatcgcttag tggccagaat ggatacagat gacatcatgg aaccacacag
     1981 attggaaaaa caattagaga tcttccaaga atatccagaa atagcgattg ttgggagtaa
     2041 tattgaagaa tttactggca cacttagcaa tgttatcgga agaaggattg tacctgagta
     2101 taatgatgaa atccgtgtgt tttcgaaaag aagaaaccct tttaatcata tgacagtcat
     2161 gtttgataaa aatgctattt tagaagtggg aaactatcgc ccattaattg gatttgaaga
     2221 ttattatttg tgggtaagac tattgaaggc gggatataaa ggatataata tccaagagat
     2281 tctagtacat gctagagcag gtgctgacat gtatgctaga cgtggaggaa gtaaatatct
     2341 tttaccagga attaaaggcc gatatcgcgt atggaaagat ggtctaggat cactaaaaga
     2401 ttttctgata gttatttgcg gacatcttgt aattagttta ttaccgaata aaattagggg
     2461 aaaagtatat gaatcaaaac ttagaaaaac aaactaaact ttctataatc ataccttttt
     2521 acaatgcaaa gaatacttta agaagagctg tagacagcgt cttaacgcaa ggttatccca
     2581 atattgaaat cttgttaatt gatgatggat ctacagatgg tagtagtgac attggtgacg
     2641 aaattgctaa tctagagagt aaagtagcat gttatcactt ggaaaacgct ggtccatcag
     2701 ttgctaggaa ctatgggata aaagaagcaa ctggaaattt ggtaggattt ttagatagtg
     2761 acgattattt tttacctgat tgttttaata atgttttgaa agactaccat aggaatcacc
     2821 ccgatatcat tgcttttgga ttaagtaaag gaaaaaatat agaaaatgca aatttatttg
     2881 ttcccgaaaa gaagattggt tgttcaagtg aagaagcaat tgagcaaatg tttagaagta
     2941 aggcagtgga cttttacgta tggaataaat tttttaaaag ggaattattc aatcatatag
     3001 tttttccaga aaataaatta tatgaggata tggttcctat gtatgaagct tttagggcgg
     3061 ctagtaaaat tgatattttg ccattttcag gtatttttta ttatcaaaat aacgatagca
     3121 tcgtttatca gtcttttaat tcaaaacagt atgataatat tttgcagaga aaaatattag
     3181 ttgatgaagt aaagaaagat tatcctcgac ttattaattt ggccaatgct cggttagtag
     3241 acggatatct ttcaacaggg tttaaaatta ctggcaaaaa tcaaaatcag aaagaaaaac
     3301 aaaaatatta taatcagtct aaaagagaaa taaaaagtat atattcaaaa ataagaagta
     3361 acaatgagat atcaattgct aaaaaattag ctttattttt atacttaatt aatcc
//
LOCUS       DACCQI010000178         3376 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCQI010000178 DACCQI010000000
VERSION     DACCQI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.187"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..864
                     /locus_tag="IVO76_002700"
     CDS             <1..864
                     /locus_tag="IVO76_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7430625.1"
                     /translation="PMSQMMDFLSIFTDGMVAGSRILKIMDTEEYAPQQNPGADQMIT
                     SGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGSGKSSIINVLMRFYEFYE
                     GQILIDDHDIREYPMEELRKKMGMVLQDAFLFYGDIAGNIRMMNPAITDEQIRHAAEF
                     VQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVTDPKILVLDEATANIDTE
                     TEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGHIVEHGNHETLLAQNGLY
                     ADMYKLQSSGI"
     gene            1190..2041
                     /locus_tag="IVO76_002701"
     CDS             1190..2041
                     /locus_tag="IVO76_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7430626.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSIHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFSCIEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            2138..>3376
                     /locus_tag="IVO76_002702"
     CDS             2138..>3376
                     /locus_tag="IVO76_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7430627.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVPFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTETAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASG"
BASE COUNT         1064 a          580 c          769 g          963 t
ORIGIN      
        1 ccaatgtctc aaatgatgga ctttttgagc atcttcacag atgggatggt tgctggtagt
       61 cgtatcttga agattatgga tacagaagaa tatgcaccac aacaaaaccc tggagcagat
      121 caaatgatta cttcaggtaa gattgagttt cgcaatgtaa gcttttctta tgatggagag
      181 cacaatgtat taaacaatat ctcttttgtt gctaatccgg gagagaccgt tgcattagtg
      241 ggacacaccg gaagtgggaa aagctcgatc atcaatgtgt tgatgcggtt ttacgaattt
      301 tatgaaggtc agatattgat tgatgatcat gacattcgag aatatccaat ggaagagtta
      361 cgtaagaaaa tggggatggt cttgcaagat gctttcttgt tttatgggga tatcgcagga
      421 aatattcgga tgatgaatcc agctatcaca gacgaacaga tacgccatgc ggctgagttt
      481 gttcaagcaa acaaattcat cgatgaatta ccaaaaggat atcactcaaa agtgatcgaa
      541 catggtgcca gctattctag tggacaacga cagctgatca cttttgcgcg aacaatcgtg
      601 actgatccta aaattctagt attggatgaa gcaacagcaa atatcgatac tgaaacagaa
      661 ggattgatcc aagaaggatt ggcgaaaatg cgacaaggac ggacaacgat tgcgattgcc
      721 catcggctgt caacgattcg agatgcagaa ttgattttag ttttagacaa aggtcatata
      781 gtagaacacg ggaatcatga aacacttcta gcgcaaaatg gtttgtatgc agatatgtac
      841 aaactgcaaa gcagtggtat ataaaagatg ttgtgacagt agcattttga cctaagcact
      901 agagtgaaaa taaaacatag cttcccatat ttttcatctg tgagagttag tgtcgtagtt
      961 accagattta aaaaagacga cgtcagctag tcagaagtgc ctaatagccc ctaagaaagc
     1021 gttgacaaaa tcgtttgctt tcgataatac aaagaaaacg atacaacttg gatagtgatt
     1081 gcctaatgca agctaaacct gatgagtctc ccatggtttc atggacggct ttttgggttt
     1141 ttctttttgt tttggataca gccctatata tcatggaaag gagcaaaaca tgctgattga
     1201 gaaagtactg aataacaacg tcgtaataac cagagatgaa agaaatcagg aaatgatcgt
     1261 gatgggaaaa ggtttggcat tcaaaaagaa agtgggtgac ccgatttttg actcatgcat
     1321 cgacaaaaaa tttcagttga caaatgacgg actttcacaa cagttccaag aattggcaca
     1381 agcgattcct cttgagtact tagaaatttc ttgtgaaatc attgaatata caaaagagga
     1441 tctacaagtc catttgaatg attctgtgta tattgcattg acggatcatt tgtatacagc
     1501 tatcgaacgt caaaaaatgg ggatctttgt gccaaatgta ttgctgtatg atatcaaaca
     1561 actgtttcct aaagagtatg ccattgggaa aaaaacagtg aagaaaatct cggataaata
     1621 ccacatcaag ttatcagaag acgaagccgg atttatcgca ttgcatttag tcaatgcaca
     1681 aggcattcaa agccagcaag acaataccta cctgtttacc acaacaatac aggatatttt
     1741 aaatatcgtc cgctattact ataagattac atttgatgaa gattcaatcc atttttttcg
     1801 attcgtcaca cacctacgtt tttttctgac acgcgtccta gatggagaga aatcagaagt
     1861 gggagaagtt tctgaggaat tgttccaggt tattcaaaaa aaatataagg acacgtttag
     1921 ttgcattgag aaaatagcag actttttaac cggtgctttc gactaccaga tgtccacgga
     1981 tgaaaaaatg tatttaacga tccatactgc gcgtttagtg catgagaatc gttgtaaata
     2041 aataaaaaag gaggacaaat taacaagaac gtatgaaaat aagggtagtg acattcagtt
     2101 ggctaaacct tccactaatt aattggaggg aatagaaatg ggaaaatacc aggaattagc
     2161 agaaaacatc gtaaagaata tcggaggaaa agaaaatatc cgtagcttga cacactgtgt
     2221 gacgcgactt cgtttcaaac taaaggagga aaataaagca aaggatgatg ttttgaaaaa
     2281 gatggaaggg atagtcacca tcatgaaaag cggcgggcaa tatcaagttg taatcgggaa
     2341 tcatgtgcca gatgtctatg cagaagtact gacagtagcc gggatttccg atgaagcaga
     2401 agaagaggtt tcttcaagta gtaatctgtt caatcgatta atcgacatat taagtggctg
     2461 tttccagcct tttttaggtg ctttggctgc agcgggaatg gtcaaaggat taaatgcttt
     2521 gttcttgttt ctcaaatttt atactgaagg atcgggtacg tacatcatgc tgaatgggat
     2581 aggggatgca attttttatt tcatgccagt gatcttaggg tacacagcag cgaaaaaatt
     2641 tcatctgaat ccgatggtgg ggctcgtgat tggtgcagca ctttgttatc cgactgtaca
     2701 aggagcagca ctacaaagtg gtttccctga tggcagtcct gcgccttata gcttgctggg
     2761 tcttccggct tatgaaacat ttttgggtat cccatgggtg ggcagcagtt ataccagtag
     2821 cgtcgtacct gttatcttca ttatcgcatt tgccggacaa gtccaaaaaa ttgccaagaa
     2881 atggattcct gcagtcgtcc agaccttttt ggttcctttt tctgtgttat taatcgcact
     2941 tcctgctggc tttttgatta tcggtccgat tgtcagtata ctgacagatc tattaagcac
     3001 gggattccaa gcattgatgg acttttctcc agctgtgtat ggtgcagttc tagggtttct
     3061 ttggcaagta ctggtgattt tcggacttca ttggagtatt atccctcttg cgattatgca
     3121 gctagcgcaa aatgggtgga gtcagatgtt agtaggcgtt tatggcgcga gttttgcaca
     3181 gacagcagtg gtattggcga tgtatttcaa gttcaaggat caaaaaatga aggcgctttg
     3241 catgccagct gtgatttcag gaatcttcgg cgtaacagaa acggctattt atggaatcac
     3301 cttaccaaag aagaagccat ttatttactc gatgattggt ggtgctgttt caggaatcta
     3361 tttgatggca tctgga
//
LOCUS       DACCQI010000179         3316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCQI010000179 DACCQI010000000
VERSION     DACCQI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.188"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1155)
                     /locus_tag="IVO76_002703"
     CDS             complement(<1..1155)
                     /locus_tag="IVO76_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7430628.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..>3316)
                     /locus_tag="IVO76_002704"
     CDS             complement(1158..>3316)
                     /locus_tag="IVO76_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7430629.1"
                     /translation="SEDGKLLHLYYGENLPDNDYSHLIEMHHRPMTTYRNENDLLYSL
                     EHLKQEFPEYGTTDFRHPAISLLQKNGSRLSDFVYQGYEVEEGKPKLEGLPATYVDNN
                     DESKTLKVYILDSVTNVQVELLYTIYRDYPVITRSARVMNKGKDIQIIENISSLSLDL
                     PDANYEWQQLSGAWGRERTIKSRTLQQGIQSIESTRGISSHQHNPFVTLKRKNTDENQ
                     GEAIGAALVYSGNFLIQAEVDTWDVTRLQVGINPFGFNWKLNPGEIFTAPEAILVYSS
                     KGLNAMSQTFHQLFRKRLARGKWREKDRPVLINNWEATYFDFDEEKLVNIAKKAFDVG
                     IELFVLDDGWFGERENDCAGLGDWHVNPKRLPRGIGSLSEKIRSFGMKFGLWFEPEMV
                     NKDSELYRTHPNWIISTPNRKPNHGRNQYVLNFGLDEVVENIFDQMCKIIDESNLDYI
                     KWDMNRPLTDVFDSHLSADQQGEVFHRYVLGVYRLYEKLITKYPNILFESCSSGGGRF
                     DPGMLHYAPQAWASDDSDAIERLKIQYGTSMLYPLSSIGAHVSIVPNHQTNRITPIKT
                     RGNVAFFGAFGYELDLNELSEGDLRIVKEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQ
                     TAWMVVSHDKKTAIVAHYKTLNEVNAPYRRLKLKGLDNDGLYNVGQNSLRSGMELMRA
                     GLVCSDASSGQINDQNRRPETFDFDSKIWVITQEEK"
BASE COUNT         1075 a          624 c          482 g         1135 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgata
//
LOCUS       DACCQI010000180         3314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCQI010000180 DACCQI010000000
VERSION     DACCQI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.189"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            488..1474
                     /locus_tag="IVO76_002705"
     CDS             488..1474
                     /locus_tag="IVO76_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP7430630.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            complement(1599..2033)
                     /locus_tag="IVO76_002706"
     CDS             complement(1599..2033)
                     /locus_tag="IVO76_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7430631.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            2148..3122
                     /locus_tag="IVO76_002707"
     CDS             2148..3122
                     /locus_tag="IVO76_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7430632.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
BASE COUNT         1090 a          599 c          677 g          948 t
ORIGIN      
        1 gccttctgct atttgactgc ggcatgaatt gcccgtacat aagaaataga tttttttcat
       61 ttgatttcct ccgttatgga ttagtaaatg tcactgttta tctaaagtaa actctatgtg
      121 atggagcgaa aaaacaatgg gtttcttagt ttatatattt ctttttgttc agcgtataaa
      181 tcgatgtgta caaagaacta atcttgtctt ttttaccata aatacatgtt agaaacaatc
      241 attttgagtc aaaaatcaac gatgtaacag actgttaaaa gagattaaga cacggcagat
      301 gatgaagaaa gtaggggtgt agtaagccga tacatttctt gattgtgagg cgagaacaat
      361 tattcgtctt ttaagaaaaa aagtagtaga atcagttcat attaaaaata aaaaaccttc
      421 catttttgta ggtttaaagg aggtcattac cttgaaaaaa atcggctttt taagctttgg
      481 acattggatg gagcaaggat cgcttgtgaa atcagctcaa gatgcttatt tgcaatcgat
      541 tgatttagcg gtggaagcag aaaaaatcgg agtggacgga gcgtttttcc gcgtacatca
      601 ttttgcccct cagattgggt cgccgtttcc tttgctagcg gcaatcggcg caaagacaag
      661 taagattgaa atcggtacag gacttattga tatgcgatat gaaaaccctt attatatggc
      721 agaaaatgcc ggattgacag atatcatcac gcaaggaaga gtgcaactag gagtggggcg
      781 tggttcacct gaacaagttc tagatggctg gaaatacttt ggttatgatt acagtgaaga
      841 agaaatcaaa gaggtaacac gggaacgcac ccttgaattc ttggaattac ttgaaggaaa
      901 accatttgaa gaaccaaatc ctcaaccaat gttcccacaa ggatcaggaa aattacggat
      961 agaaccttat tcagaaggat tacgacaaag aatctggtgg ggagctggtt cgcagcaaac
     1021 ggcaatctgg gcagcacaac atggaatgaa tctgcaatca tctacattag tcaattacga
     1081 atcccacgag ccattccata tccaacaagc aaaccaatta cgtgcgtata aagaagcgtg
     1141 gaagcaagca ggtcatgatt ttgaaccaag aacattagtt actcgctcga ttcagccaat
     1201 caccactgat ttggatcgac gcttatttgg acaagacggt agtccgcaac aagatgctgt
     1261 cggctatctt gcttatcacc gaaacccaac gatttttgga aaaacttttg ctggtgaacc
     1321 tgatcaatta atcaaggagc tagctgaaga tgaagcaatc aaagaagcag acacagtact
     1381 tgtcacgatt cctaatactt tgggggtaga atataacatg cacttgcttg aaacaatcgt
     1441 aaaagatatc gctccagaat taggatggcg gtaaaaagga tatttttgta agtgcaggat
     1501 acttgagtga aaaaataaac acttctaaca atcagttaga ggtgtttatt catgttttca
     1561 tgaagttcat agagaaggag agtgagttat ccgtttgttc aatgagaatg tacataagct
     1621 tcttttacat gttggggtaa atcatcagga atcattgtca atacggcttc ttcattgccg
     1681 gcttttattg cttcttcata gtaccagctt ttgtagttta gcatagcgag tactctttct
     1741 aattttgcta tttctttttc tactacttct ttttgttttt caaatagttc tttacgaacc
     1801 tcaaatgtct tactgccttc tttgctccat tccatgaatt gcttaatgtc ttttatttct
     1861 aaaccagatt ttttcaaaca gtcaatgact cttaaagcct ctaactcatt ctctgaaaat
     1921 tttctaatac cagaaacacg ctggatatca ggaaataatc cctctttgtc gtaataacgt
     1981 aaagtagaaa caggcaatcc aaacatttca gacacttgtc caatggaata cataaaaaaa
     2041 tcctcctaaa ataaaatttt taccttgacc taaagtcaag tttaagtttt atcataataa
     2101 tacattcaaa aacaaatgtc aattttgatc aaaggagagt ctagaatatg aataaagaat
     2161 taaacatcac acaagaatgg gacaaagtat ttccaaaaag caatcaagtc aatcatcgaa
     2221 aggtgagctt cccgaatcgt tttggtatca cattagtcgc tgatttatat gaaccaaaag
     2281 aagtaagtga aaagaaattg ccggcaatcg ctgtgagcgg gccatttggt gcagtgaagg
     2341 aacaatcgtc tggtctatat gcgcagacga tggctgaaag aggcttttta acgattgcgt
     2401 ttgatccatc gtttacagga gaaagcggag gagtacctcg atatgttgct tcgcctgata
     2461 tcaatacgga agatttccaa gcggcagtag actttttatc tgtacaaaaa aatgtaaacc
     2521 ctgagagaat cggaatcatt ggtatttgtg gatggggagg tatggcattg aatgcagcag
     2581 ctatcgatac gcgggtcaaa gcaacagttg tttccacaat gtatgacatg actcgagtaa
     2641 cagcaaatgg ctacttcgat gttatggatg aaaatgcccg atatgaaatg cgtgaagaat
     2701 taaatgcaca acgcatcatt gatttcaaac aaggggacta cgcacgtgct ggaggtgtgg
     2761 ttgatccgtt gcctgaagat gctcctcaat ttgtcaaaga ctatcatgcg tattataaaa
     2821 cagaccgcgg atatcatgtc cgttctttaa actccaatga tggttggaat aggacatcag
     2881 ggttgtcact tatgaatgca aaactattgc attatattcc agaaatccgt catgcagtct
     2941 tagtcattca cggggaaaaa gctcattcac gttactttgg ggaggacgct ttcaaagaac
     3001 taaaaggtga caacaaggaa ctcatgatca ttccaggagc atcccataca gatctatacg
     3061 accagacaga catcattcca tttgaaaaaa tggcaacatt ctttgaagag aaccttgtgt
     3121 aaccaaaagg atttaatcta aaagagatta tgaaaaaact gccctgcatc aagtaataag
     3181 gacagccaaa aaagctcctc atttttgact tggttggact cattagcgta gatgcaagct
     3241 tttgaacacc gtttatacca agaggtcgtg agcctgccgt ttaactctga gtcacaagga
     3301 gcaatttcaa aaaa
//
LOCUS       DACCQI010000181         3282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCQI010000181 DACCQI010000000
VERSION     DACCQI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.190"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            647..964
                     /locus_tag="IVO76_002708"
     CDS             647..964
                     /locus_tag="IVO76_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7430633.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(1055..1420)
                     /locus_tag="IVO76_002709"
     CDS             complement(1055..1420)
                     /locus_tag="IVO76_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430634.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1628..2110
                     /locus_tag="IVO76_002710"
     CDS             1628..2110
                     /locus_tag="IVO76_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430635.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            2307..3197
                     /gene="pstS"
                     /locus_tag="IVO76_002711"
     CDS             2307..3197
                     /gene="pstS"
                     /locus_tag="IVO76_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7430636.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1137 a          499 c          598 g         1048 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatctt aaaattatct tcttactgtg aataagataa
      421 taaaatttgt atttgtcctt tttaggtaac ttattttaat ctatatacct ttctggatca
      481 acgaaagtat actttatata gtcataacgc cgatggtaac tatgcactgt aacgataatg
      541 ctgctaaaat atctttttac gaaaaacatc acaaggaaaa acttaactat ggtattatct
      601 aaacataatt ttttcggtat ttaatattta attaggaggt aatattttgc ttttttatag
      661 taattttatc ttaatagtag cgatattatt acttctgaat atatggattt ttgataggag
      721 tagaaattca agtataggtt ttagaactaa acgaagtttg tcttctaaaa aaaattgggt
      781 atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt cttcaacact
      841 ttattctttg aatattatag atgtttccac ctcaaattca atatctatta ttggaatcat
      901 tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa gaagtaaaaa
      961 atagaaaaac tagtgatagt tttggtgata taagaaaaac atatatgtat aaaaagaaga
     1021 aagtagcttc tctttgtaca tatatggttt gtttttatgg atgccgagct tttttataag
     1081 ataaggctac cgcccataac gacccaagga tttgtggaac taagtaaata cctgcaagaa
     1141 gcaagatcag ctgggtcggt tttaaaggat gatagaagta acgccattca gtaaaattgg
     1201 cgcaaaataa ataggaagtg acagtagata taatattggc aatgaataaa gatataatac
     1261 gcctgttcct ttgaaaataa tatccagcaa aaagagataa aaatatcaat gtgatgaacc
     1321 cgcttacgga atgataacga aaatcaagca gtaaggcgaa atgcacataa ggaacaaaag
     1381 ataaaagcaa cggaaggttt ttccagcaag tttttatcat ctttcatcac cacctaacaa
     1441 gtgttattat tttgttagtt ttattgtaaa ttgaatcctg caataatcaa ttactttttc
     1501 attatttcta gattaaatga ataaactctc atgccattat ctctaaaaaa aaagtttttc
     1561 cccctaattt ttttagatag aaaattgtat agtatgatga gagaaaaagt agtttggggg
     1621 aacgttaatg agaagaaaag cagtaatcga tcggctttat ttattttttt ggttttttcc
     1681 tatcgttttg ctacctttta catgggtaaa tgctgttgta caaggtgcgg agatattagg
     1741 cctgatccaa ggaagcggat ttgtggtttc taggaatttt ccagctttga ttgttttcta
     1801 tttagtgata agtgtaggtc tcttattacc aaaatctctg atcaatcact acaattggca
     1861 gctgctttac ttttcttgct tattagtatt tacatctatg ttttctttgc tgcctcgctt
     1921 gattatcggc cctaatggtt tgagcgagag tggattgaag gatttagctt attcctccat
     1981 gtatttcttg acgatcaaac catggttcta tttagtaatt gcctcattta ttcttgcaac
     2041 atttttttac ttctggacag aaatcaggat aaaaagtgaa cgatctgtat ctataaaaaa
     2101 taactattga tggtggatca tcagtaaaaa aacatatgag ctgcgtaaaa tgcatgtttc
     2161 tcatatgctt tttttattat tatgtaaaga atgcgaaaaa cgtttacatg atttttacat
     2221 aacttaacta tatttttaca gcaaccatac gagggattta cagtcgactg ataaacttta
     2281 gttgtaaata agaaaggagc aataaaatga aaaaagtatt gtttactcta ggtatgatgg
     2341 gaattctttt agccggatgc ggctcaggat caggaaatac agatggctct gccacacaaa
     2401 ataattcaag tgactcaaat gaacaagtaa aaatcgttgc ggtaggttcg acagcattac
     2461 agccgttagt cgatgcagca caagaaagct ttgtacaaga aaatccaaat tatcaaatct
     2521 ctgtacaagg aggaggaagc ggaactggtt tgagccaagt cgaagctgga gcagttacta
     2581 tcggaaactc tgatgtattt gcagaagaaa gagacggagt tgatgcttca aaattagtgg
     2641 atcataaagt agcagtggtc gggatggcac caatcgtcaa taaagataca gatgtcaaag
     2701 atatcaccaa acaggaattg attgatatct ttacaggaaa aatcactaac tggaaggaag
     2761 ttggcgggaa agatcaaaaa atcaacgtcg tgaaccgtgc aaacggcagc ggtacccgcg
     2821 cgacattcga aaaatgggga ctggatggtg ctacccctgt gcagtcccaa gaacaagatt
     2881 catcaggaac agttcggcag ctggtaagtc aaacaccagg agcaatcagc tatttagctt
     2941 tctcatatct cgatgattcc acacaagcac taagtatcga cggtgtagaa ccaaaagaag
     3001 agaatgtagc agataacagc tggggaatct ggtcatatga acatatgtac acgaacggaa
     3061 aaccttcccc tgaagtccaa aaattcttag actacatgat gacagaagaa atccaagaag
     3121 gacctgtcaa agaattgggc tatttaccaa tcacaatgat ggaagtagaa cgtgatcacg
     3181 aaggaaatat caaataacta gcagataaac atgccacggt agtcgttact attaaggaaa
     3241 aatcaactat gtttccccac atatacattt ttcctttaat tg
//
LOCUS       DACCQI010000182         3020 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCQI010000182 DACCQI010000000
VERSION     DACCQI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.192"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(106..1053)
                     /locus_tag="IVO76_002712"
     CDS             complement(106..1053)
                     /locus_tag="IVO76_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7430637.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IVO76_002713"
     CDS             1299..1763
                     /locus_tag="IVO76_002713"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7430638.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IVO76_002714"
     CDS             complement(1825..2868)
                     /locus_tag="IVO76_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7430639.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          997 a          599 c          579 g          845 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacgg
//
LOCUS       DACCQI010000183         3007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCQI010000183 DACCQI010000000
VERSION     DACCQI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.193"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(276..692)
                     /locus_tag="IVO76_002715"
     CDS             complement(276..692)
                     /locus_tag="IVO76_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430640.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(695..979)
                     /locus_tag="IVO76_002716"
     CDS             complement(695..979)
                     /locus_tag="IVO76_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430641.1"
                     /translation="MDILVRNIDPKFVATIDKLCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            complement(1679..1774)
                     /locus_tag="IVO76_002717"
     CDS             complement(1679..1774)
                     /locus_tag="IVO76_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7430642.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            2427..>3007
                     /locus_tag="IVO76_002718"
     CDS             2427..>3007
                     /locus_tag="IVO76_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7430643.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALREL"
BASE COUNT          958 a          477 c          565 g         1007 t
ORIGIN      
        1 aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg gcaataaaat
       61 cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga tttctctgcg
      121 taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag caagtagaca
      181 agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg gggcttctaa
      241 ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa atttgttggg
      301 tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt tggtcgattt
      361 cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata tactttagaa
      421 tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc tgcgatgatt
      481 tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt gctggtagtg
      541 gtcgtgttgg tatcgggttc ataactcata cataaaaagc gaatggcttc cgagtagtta
      601 ttgatatctg gatttttttc gatgatggaa tctaaaacgt caacatgact agggtacaaa
      661 gtaacatgtt tttggacaga agtttttctc atggttatat cattcctccg ataagcattt
      721 caatgatttg attatttgtg tagacatact cgcttaatat ttgagtattt tctgtttgga
      781 tagcaataaa tttatcgagc aataaatcga attggtcttt tttgtaagtc gtaagttgac
      841 ggtcaaaatc actttcgatt aaaactttta gatatttatt tcgactccat ttttctcctg
      901 ttttattcgt gagttcttgg caaagtttgt ctattgttgc aacaaacttt ggatcaatat
      961 tccgaactaa aatgtccata aaaaatctcc ttttgcaatt atgtataatt tatgtatacc
     1021 atacaaatac tatatttgta tagctgtttt ttgccaaaat gctatacata agctacccac
     1081 gatggcgaaa aagtgatatc aaattttttt ctgagaaacg tgataggcac agcctatcta
     1141 cccacttttt ttagaaagtg ggtacacgtt tccttatgct ctttaaaatt cgggggggag
     1201 tgctgcacgt gtaatcgata accaaattta acagacgaaa aattgttgga atataaaaat
     1261 atgacacccg aaagaaaacg atcgttggaa aaattaatgc aactagtgaa cgaagacggc
     1321 ggaatttcag acgcagcttt tgacgcatgg agcgaatttc atggagaata taattaagtg
     1381 ggtttaaatg gctctaaaat cgcttctaag agttttttag cttggaccaa tgaaatacac
     1441 caaaaactaa ataaaacgtc ttaaatcgaa aaatacgagc tctcagagcg actttatttg
     1501 taaatgttgc atataagaaa taattacgga ttttagactt ttgtaggata tttttgaaat
     1561 aaatatttat ttctgttata atttgattgc aaataagtga acacgaaaca cacgaaatcc
     1621 ccagagaggt cggattctct ggggcttttt ttgtgggctg agtcgcctta attaagtttt
     1681 acttcttgtt tcggttattc aaccagtaac tgaacaaagc gcttaaaagc ccaacaagaa
     1741 gggagcaaat aaagttagtg aacacgaaac acacctcctt ccagtcgcaa gactgtcgga
     1801 taggcgacaa tcaattatac aataaaaaag caaaaataag cctaagtttt ttattgcctt
     1861 ggggttgtta gggcttgccc tgaccgcctg taaggcgctt tagaacgaag gacatagttt
     1921 ttatcgaatc ttgtcttaga acggcttaga cgagctctca ggacgccaca aaggagtccc
     1981 taatgaatta tttttggcga aggagcctaa aggcgactgc tgcaagtaaa atgtgagagc
     2041 gtagcgatca ctccttctta cagcgaccat agggagctag attggcagtt taatatcatg
     2101 cgcgaacctg tttattttaa aaagtgggtg attttttggc gggaaatttt gctcgcgcgc
     2161 gcgcgtttat tttatttatt ttttaaagat tttttagaac cctttttagg gggcagttga
     2221 gccttactct cccaacggat acagaagggt actaacaact ttttatctga gtactaacaa
     2281 ctttttatct gagtactaac aactttttat ctgagtacta acaacttttt atctgagtac
     2341 taatatcctt ttgtataata acaaccaacc tatttacgag ttggttgtta aaggttatta
     2401 tttatttgta aggggggaag aaaggaatgg atgaaaaaac ggaattaaca gtcaaatatc
     2461 aaaatgaatt gaatttagta ccgctgaaaa atttcaatgc taagcagatg gatttatttt
     2521 ttgcattatg cgctcgtatg aaagatcgtg gtttgaaaaa agttagattt tctttcgagg
     2581 aattaaaaga gttaagtgat tataagatga ctgctacaaa agcctttgtc ggtgatttag
     2641 aaaagctcta taaagatatg cttaatctta cttaccgtac tgaagatgag gattctattc
     2701 gctactttgt tttgtttact ggttttgaaa tcaataaaac aaaaaagttt gttgaagtca
     2761 gtgttaatcc ggacctagaa tatattatta atggtttaac agctgaattt agtagatttg
     2821 aattatctgc tttcacgtcg ttgcgttcta cgtatgcaaa aactctattc cgactgctta
     2881 tgcagtaccg ttctactggt tattatgtag ttcgtattga agagtttagg agcttaatgg
     2941 atatcccaga atactatcaa atgggtaaca ttgatcaaaa agtattaaat cctgcattac
     3001 gagaact
//
LOCUS       DACCQI010000184         2964 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCQI010000184 DACCQI010000000
VERSION     DACCQI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.194"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(12..287)
                     /locus_tag="IVO76_002719"
     CDS             complement(12..287)
                     /locus_tag="IVO76_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7430644.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            395..1159
                     /locus_tag="IVO76_002720"
     CDS             395..1159
                     /locus_tag="IVO76_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7430645.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLADVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            1282..1785
                     /locus_tag="IVO76_002721"
     CDS             1282..1785
                     /locus_tag="IVO76_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7430646.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            2193..2840
                     /locus_tag="IVO76_002722"
     CDS             2193..2840
                     /locus_tag="IVO76_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP7430647.1"
                     /translation="MEQTIKPYQYFFIIREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
BASE COUNT          993 a          520 c          625 g          826 t
ORIGIN      
        1 ttttcacaac cttatcccac cacatcaaat ttctttcgtt cctcaatcat cggatcttct
       61 tggacatcca catactgcac tcgtccactt gggctcggag acttcttgac ttcttcgata
      121 aatttttgga tgatgtcgtc atttccgatt gcttcgattg tgactgagcc gtcgtcttcg
      181 ttttttaccg ttccggtcac gcctaactga tcagcaacca tcttggtcat atagcgaaat
      241 cccacgccct ggacacgtcc ttggacattc attttaactt tgcgcatgtt cgtcaccttt
      301 ctttcctgtt ttataaatca atgataccga tttttttagg atgaaacaag ctcaagcacc
      361 tattccatga tataatcagg tgaggtgaag gaatgtgaaa gagatccaat cagcaaaaaa
      421 tccaagaatc aaagaattaa aaaaactaca taaaaagaaa tacagagaag aaaaggcaga
      481 atatcttttg gaagggttcc atctgatcga agaagctgcg tcgtctgatg cagaaataaa
      541 agaacttttt gtgacacaga gaggtatgaa agaatgggga gactggctgg ctgtaaacaa
      601 agagatttct acttttctag tttcagatga ggtcatgacc gctatgtcag atctacctac
      661 accgcaaggg atcttagcag tgatgacgtt aacatctgaa gatacgccag atttttcagg
      721 aggctggctt ttactagata atgtacagga tcctggaaat gtgggaacga tgatccgtac
      781 ggcagatgcg tttggtatgg ctggtgtttt attaggtaaa ggaacggcag atatttatag
      841 tacaaaggtg ctgcgaagca tgcagggaag caattatcat ttgcctgtgt tacaaaaaga
      901 tttggcagat gtaatccctg taatgaaagg caaagggttt acggtgtacg gtacagaatt
      961 aaatgaagaa gcacatgcat tagacaaagt agaaaaaaca gaggatttcg ctataatcat
     1021 gggaaacgaa ggtcagggag tcagtcagga aatcttgtct ttgactgatc agaacgtata
     1081 tatcccaatg aaaggaaatg cggaatcatt gaatgtcgga gtcgcagcag ggatcgttct
     1141 ttatcatttt tctttatgat taaggaacta ttaagcttta gctattttat ctagagcgac
     1201 tagaataatc cgaacactgg tggtatactt agatcaattt cagaagaaaa tgaaattcaa
     1261 aaaggtggca gatcgataat tatgacagaa aaatggcgtg aagacgaaga atatatgtct
     1321 tatgtacaag atcttttaga aacagaagca gtgaaaaaat tagcgaatta cacacagcat
     1381 gttcattcca ctcgtttgga gcattccatc agtgtttctt actatagtta tctgctcgca
     1441 aaaaaatggg gtggcaatgc taaagcaaca gcgcgtgctg gacttttgca tgatctgttc
     1501 tactatgatt ggcgtacgac gaaatttgac gaggggacac atgcgtatat ccatccaaga
     1561 attgctgtaa agaatgcaga aaaaatcacc gatttgtcgg atcttgagcg agatatcatt
     1621 ttgaagcaca tgtggggtgc aacgatcgca ccgcctaagt ataaagaagg atatattgtg
     1681 acattcgtgg acaaatactg cgcagtcaaa gaagcggctc agcccatgtc tgcaagtatg
     1741 cgtaagagat ggcaacgtta ctttgggaaa gaatcctcga tttaacaggt aaaaccaata
     1801 atagaccata ttgaggttgt gacagaagtg gtcagcttca agaaataaga aggaatttac
     1861 gaaaattact tctcaaattt ttgtgaagtt tcggcttatt actgcagatg caagcttttg
     1921 aacaccattc attcgaaaca agggactttg acacgacttt tgtcacagtc cttttttctg
     1981 tttgcagttg actatctgtt caagatacgg tttactttta tttatcgaat gaatcatgta
     2041 catagaataa taagcattaa agggggtgat gataagatgc aaaacgtatc ttatttaaaa
     2101 gagtccagga tcttcaggta acacatactt tgcagaagat cacttttttc tacaaagtat
     2161 gcactttggt tagaagaaag gactttaaaa tcatggaaca aacaataaaa ccataccaat
     2221 atttttttat aataagagaa gtagaacagt tattaaatgc gtactcatct gtcaatgatc
     2281 caaaaacggt acaaacagtt caggcactag cagctgaaaa gattcgagac atattagatc
     2341 acgaactgcc agaaatcgat acatttttaa cgacagtttt agacgtaaaa ctgacaaaag
     2401 cgcaagcaga aagagcattg gaggagctga agcaaatcgt tcagccgttt atgcagccta
     2461 gcaaaccaca aatcgagaaa ttattccgaa aagtgaaaaa actcaaacaa ccagcttgga
     2521 atgaaatcga cttgcaagaa catacgtaca tcggctggaa tgatccaggt acgcagaaaa
     2581 aattcatggt ctattacgaa gatgaaaaat taaaagggat tagcggtact ttatcaccaa
     2641 cgataaataa gggaatctgt gcaatctgtc agaaaacatc gaatgtttcg ctttttcttt
     2701 ctacaacgaa agcaggttct gatggcacgt atacgaaaaa ggggaattat atttgtcatg
     2761 atagtgacca atgtaatcaa cagttaacga aattggctcc tttctatgaa tttgctgaac
     2821 gtgtaaagaa agaacgataa ttttattgtt ttttacagaa aaatatgcta tcttttaatg
     2881 gaagtgaata ggtgatggtg ttcgccttta aatgctaacg aaagatagct aatgacgcct
     2941 actaaagtct ggggacagcc cctt
//
LOCUS       DACCQI010000185         2892 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCQI010000185 DACCQI010000000
VERSION     DACCQI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.195"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            433..600
                     /locus_tag="IVO76_002723"
     CDS             433..600
                     /locus_tag="IVO76_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7430648.1"
                     /translation="MVYLNKKQTCEFLGIDNNTLDKWIRIYNFPVIKIDGIIRFDVED
                     IKGFMDTFKKI"
     gene            674..1900
                     /locus_tag="IVO76_002724"
     CDS             674..1900
                     /locus_tag="IVO76_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7430649.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(2058..2675)
                     /locus_tag="IVO76_002725"
     CDS             complement(2058..2675)
                     /locus_tag="IVO76_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7430650.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
BASE COUNT         1001 a          476 c          435 g          980 t
ORIGIN      
        1 attttaaatt tttacatacc tgtttatgaa aaactaaata atctgtcaaa aaaatatgat
       61 tgttcatttc taaatatgtt ttccaatttt aaaatctttt ttagcatttt tgtttccttt
      121 tcatatggtt tttcgttgtt aatagtgact aggatgatat tcctattttg accattgaca
      181 attaaatata atttttgaat aacgttccct gctattcgag ctaagaattc aagtacgcca
      241 tgacctattt agcgataact acttagagat ttctttttgt tttgggaaga caaaatgcca
      301 tgacaatagc agtagataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga
      361 aattcctatg tgcttaccca agtcaattca aaaattttag ttcatttata aggggatgat
      421 tttattaaat caatagtcta tctaaacaaa aaacaaacct gtgagttttt aggaattgat
      481 aacaatactt tagataaatg gattcgtata tataattttc ccgtaataaa aatagacggt
      541 ataattcgct ttgatgtaga agatatcaaa ggctttatgg atacgttcaa aaagatttga
      601 attttacttt tgactgcata tactaaaagt agcgaaatat actgccttac ttttttatta
      661 aggagtgagg aagttgccta cgaagctttc aaatggaaaa tataaaacta atttgagata
      721 tccaaaaaaa tttaaagaaa ttacaggtat cagttcagaa aaattccaaa aaacatttcc
      781 aactaggcaa ttggctatta aagccgaaaa tgaaatgaaa aagaaaatcg caaaagtctt
      841 aagagaggag aatgcaaatt cattagagct caaaggtcaa ataaaattca aggagtttta
      901 taaatctaaa tggcttccta gatatgagtt agggcaaact actagatccc ataaagctcc
      961 aagcgatata accatcatta atacaaagaa tttatttcga ttgcatattc tgcctatatt
     1021 tggtgagtat gccatgagct atcttaatgc aaataccgaa ataatatcag atgaactcac
     1081 aaaaaagtcg aaggaatatg caaacataaa aatcattaaa tgctatgttc gttccatgtt
     1141 tgatattgca gaggttctaa attatattga atttaatcgt acaacaaaaa taattcaaag
     1201 tattactgca cctaagaaag ttgctctgga agaaaaaaga atacgagaag gaaaacaagc
     1261 tttatcttcg aaagaactca ctgattggtt agacgctgta agagatgatt tcaatgatca
     1321 attactttcc tttcacgatt acactctttt tatgcttact ttatatcttg gggatagaaa
     1381 gagtgagacc tatgctttgc aatggaaata cattgatttt gaaaaccaaa cagtccgtct
     1441 taaatatgcc ttggataaac acacaaaaaa gaaatttaca aaaggtcgaa aagatactat
     1501 tatccaagtt ccggaaattg taatgacact attgcaagaa tggaaatcag tgcaagctga
     1561 acaactattg aagttgaaaa ttaaacagac acctgaccaa tatcttttta gttactccaa
     1621 accttctggt gaagtaaatt gtccagtcca tactgattac ttaaattatc gaattaattc
     1681 tataaaaaaa agacatcccc atttggttca tttaactcct cataaacttc gacatactta
     1741 cgcaacttta gctagacaag gtggggctga tatgaatcaa atttctaacg ctttgacaca
     1801 ttcaggtatt tcaactacaa aaatttatgt gaatacacct gatttagttg ataaagctgt
     1861 gttcgaagcg tttcagcgag gattacaaaa atctaattaa tgtgttctag aatgtgttct
     1921 aaatataaaa aaaagacact ttccaaataa ttggaaagcg tcttgaacgt tgatatataa
     1981 aggatattaa cgttttgaga attgagatgc tttacgagct ttcttaagac ctggtttctt
     2041 gcaaaattat attagcctca actgaaagat ttattttttt gctccctaac ttcattagta
     2101 ataccatgtt tcattcgata tcttctaaac gttctacgat taatccctgt tagctcttca
     2161 acttctttat ctgtagatcc agtcaaaaac aaattaaatg ctaattgaag tcgcggatca
     2221 ttctcacgat attttagcgt acctcccttg tatttccctt ttgctttagc gagtgcaatt
     2281 ccttgttgtt gcgtttcgcg aatataccga cgttcttctt cggcctggta cttgtatagt
     2341 tcgataatta agttattaag taatcgacgt aaattatcat cagtgatacc tgttaacgct
     2401 ggcaaattca aaacttccag tgtagctttc ttcagctgaa ttaaattcat cgtttctgtt
     2461 agctctttat tatttcgtcc aagtcgtttt aattctgtca caacgacaat atcaccttca
     2521 cgtatgaatt tcatcatttc ttgaaatcct tctcgattgg tatctttccc acttgcttta
     2581 tcactaaaaa ttttatcaca ctctgataat aattctaatt gtcggtctaa attttgatct
     2641 aagctgctta ctcgagcata cccaattttc gccataataa aactccttta aaattctgaa
     2701 atccggatca tttttaaatt ctgttgccca actctgttgt catttttcta gataaatggc
     2761 ataaattttc agcgctaagt gacaaaagca actaattttt catgatagat ttttaaatat
     2821 ctcaatctat ctccaaaatt agttgctttt gtagctatag ttgactatga atatgacaaa
     2881 taattagaaa aa
//
LOCUS       DACCQI010000186         2798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCQI010000186 DACCQI010000000
VERSION     DACCQI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.196"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..464
                     /locus_tag="IVO76_002726"
     CDS             <1..464
                     /locus_tag="IVO76_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430651.1"
                     /translation="ELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQID
                     DENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIHRF
                     IRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            523..963
                     /locus_tag="IVO76_002727"
     CDS             523..963
                     /locus_tag="IVO76_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430652.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            975..1643
                     /locus_tag="IVO76_002728"
     CDS             975..1643
                     /locus_tag="IVO76_002728"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7430653.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1707..2330
                     /locus_tag="IVO76_002729"
     CDS             1707..2330
                     /locus_tag="IVO76_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7430654.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2386..2772
                     /locus_tag="IVO76_002730"
     CDS             2386..2772
                     /locus_tag="IVO76_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7430655.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
BASE COUNT         1006 a          462 c          582 g          748 t
ORIGIN      
        1 tcgagctttc tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa
       61 tgatcgatac aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc
      121 gttatcaaat agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa
      181 aaagcttttt aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga
      241 aaaaatatcc aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc
      301 atcgttttat acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca
      361 catttcgagt agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa
      421 atcggaatgt ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg
      481 atttttttgt cataataaat attagatgat ggaggagata atatgatgca aacatggtta
      541 gaaaggctaa tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa
      601 tttgatcagt tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt
      661 tggttgccct atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt
      721 cgtttcctat caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat
      781 cttgatagag tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat
      841 cgaaaaagtt atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa
      901 ggaaaaggac gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt
      961 taagggagaa aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg
     1021 aaaattactg gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag
     1081 cagttgccgg tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg
     1141 ttttgaaacg tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc
     1201 ttcctataat aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa
     1261 acaaagataa gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat
     1321 atacggaagt tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg
     1381 atccgcattt gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct
     1441 tatggcaaga tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc
     1501 atggtaatag catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg
     1561 atacgatcga tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa
     1621 ttctggacaa aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag
     1681 aagcaaagga atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat
     1741 tgtttgatac gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta
     1801 tcagtatgga caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga
     1861 tcaatgaatc aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag
     1921 aaagtacgga agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag
     1981 acaacggcgg tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt
     2041 taaaagaaca tcgattggca catctgattg aggaagtagt agggatcgat caggactttc
     2101 ctcgtaaacc tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa
     2161 cagacacgat gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag
     2221 tcgctacttg tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg
     2281 taggaaatct gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt
     2341 caaaggagat atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga
     2401 caaaaagaag ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa
     2461 acagatttga aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa
     2521 catagcatcg taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct
     2581 ggaagtgtga gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc
     2641 acacaagaag aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct
     2701 tatgaggtga cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa
     2761 aaacaagaat agacagtaaa aacatacctg cgatagat
//
LOCUS       DACCQI010000187         2745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.197, whole genome shotgun sequence.
ACCESSION   DACCQI010000187 DACCQI010000000
VERSION     DACCQI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.197"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(58..1857)
                     /locus_tag="IVO76_002731"
     CDS             complement(58..1857)
                     /locus_tag="IVO76_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7430656.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(1981..2577)
                     /locus_tag="IVO76_002732"
     CDS             complement(1981..2577)
                     /locus_tag="IVO76_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7430657.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          970 a          518 c          477 g          780 t
ORIGIN      
        1 ggtggcaggt tatgcgtttt ttgttgtata aagacttttt tcattaacga tttttgatta
       61 gccgactccc aaaacaaaat ttagcagttt atctgaataa gtgatttctt catctgtttg
      121 tttcacactt tcatttagta gtcccatctc atcggttatg ataccatcca caccataaaa
      181 catcatgcga ctcatagcat ctgaatcgtt gattgtccat acatatactt cttttccgtc
      241 ttggtgggca gaattaatga agttacggtt gatcgtacta tattccattg tcagaaaatc
      301 agcaggcgtc acagggggcc caacaatatt gaaaggtaag atgtagccca cataaaagtt
      361 tggttctgct tcttttaaat ctgaaacggt ttggtacgtc aaactttgca aaatatgatg
      421 gttctctaag ataactttgc ggtatttatg gacaaatcgt tcgacaaggt catccgaatc
      481 ttttttagag gtcttgatct caataagtaa tttttggttt agcttatgtg ctttttccaa
      541 ataagaatca aaagaacaaa caggggcttg cactccgttt tcagtaactg taagatttgt
      601 caattctttc aatgtaagat cctgtggtgt tccatttacc cctgttaagt gacgaagatt
      661 tgcatcatgc atcactacga aatgaccatc ttttgtttcc tgaacatcca tctctacata
      721 atcaggatga tagtctctgc ttgttttttc aagtgcttct aaagtatttt gcgccccatt
      781 ttgggtactc acacctctgt gagagacagt cactggtttt gtctgaacag ctgaagtcag
      841 ataatccata ttgtaaagac agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc
      901 agtgtttttc agcgcagaga aacgcaagtt gggggcttgt gggataaacc acttagggat
      961 ctcaggcaag aagccttcgt catccatgaa atcaacaatg atataaaaaa tccctacggt
     1021 agacatcacg atattgaata ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct
     1081 gatcaatgca tagtctggga aaaattgttc aaccatactt tgtgccaaaa taacaacgat
     1141 atatcctaaa gaagaaagga gtaaaatcgt accgccaatc acaataaatt ggcccgtgat
     1201 ggccaaaaga cgcgatttgg tcaatgacca gctttcccga atggctgctc gaaaaggacg
     1261 atcccgtaga atcatttccg gtaaagcaaa aatcagtcga atcccaatat agccaaggaa
     1321 aatataaacg agtaaaaagg aactgacgat gatccaacga ttagcaaaaa taaaatccat
     1381 aatgaaagcg gggattttta tttttgacag tagatcagaa ttaaaactta atccgctgat
     1441 cggtagaatc aataaaaaat aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt
     1501 cagctgaagt atcgtcaagt gtaagagttg cttcaaagag attggttctt tctttttaat
     1561 aaaaaaaaca ctcatcaaaa gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat
     1621 caaaactacg attaacgaga gtagaacccc aggatgttga gaaaaaattg aaggaatatt
     1681 gtcataagac aaatagtcaa ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa
     1741 taagggtaaa caaataaata aaataaagcc atgcatcagc aagacatcac ggaaataagc
     1801 ttgtgttcct tttaaaaagt cccaagcatt acctaagcta cgtttcaaat aattcatcct
     1861 atcacctgct cttcacatta tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt
     1921 ttgcagaccc tttcccagat ttaacattgt caatcaatga ctaattttac aaaataaata
     1981 ttatccaaga agaccactta gatattgacc tccataaatc aaggccatac tatataagaa
     2041 aatcgacagt ggtttagcaa tcaaaatgat tagtgtatat cgtttcactg acatgtttgt
     2101 caatccagcc atcaaacata acgcatcgtc aggagcaact ggaagaaaaa tagctagagc
     2161 aaacagcttc tcaaaacggt tttgattatc cagccagcca atatatttgt tatatgtctt
     2221 atcgctaatc atggaaagta tgaatggctt tccataacga cgccctagca gaaaaatgat
     2281 aatcgaacca atgcagattc caatatagtt atagataaat ccagcataag ggccaaatat
     2341 caggacacca gctgcagtac tgatccctcc aggaatgatt ggaataacta cttgcaggat
     2401 ttggatgaag acaaaaataa tcggtcctaa aatgatggaa tcacctacta atcctcttaa
     2461 cgcattcaaa tctttgaaaa cacctaaatt aataaaataa atcgtcagcg cgattgatag
     2521 gcctaatccg ataatggata tgaaatttat cagttttcga gaggcagcaa tattcattat
     2581 tcatcaactc cttagaggtt ctttgttaac tatactttac tagaaaagca actaactgcg
     2641 ccattttttg atatgacaga aaacaaacaa ttttgtaagt tcatcataag tataaaagat
     2701 tgatctcaaa aaaacatcca actaaagact tatttaataa cttgt
//
LOCUS       DACCQI010000188         2718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCQI010000188 DACCQI010000000
VERSION     DACCQI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.198"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            359..1288
                     /gene="yidC"
                     /locus_tag="IVO76_002733"
     CDS             359..1288
                     /gene="yidC"
                     /locus_tag="IVO76_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7430658.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADNKQPKNSTNNKGRNAGKQQKRK"
     gene            1391..2718
                     /locus_tag="IVO76_002734"
                     /pseudo
     CDS             1391..2718
                     /locus_tag="IVO76_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT          934 a          481 c          518 g          785 t
ORIGIN      
        1 cttaatacca agaaactgac ttttgaacac cgtcgataag gttgtttttg aggcgctttg
       61 ccccaagtat taaagacagg tagtaaaaaa gctgatctat cacttgcagt gagttcattt
      121 gactcctcta tttcgggcag aataaaaaaa gtttttagtt ttttaaataa gaaagaaaca
      181 cttggaatca ctcttttctt tccaagtatt tctttcttga ttttttgtct tttttcaaaa
      241 tttttcgcta cgaaaaagaa agaatattta tatgaaattt ctatgaagtt gattgaattc
      301 catgcgcttt tttagtatag ttaagtttgt gtaaaaatta tagaagagga agtattctat
      361 gaataaatgg aaaaactggt tattgggatc cggacttgtc agtatcctgc tctttttatc
      421 tggctgtgtg cggatggatt caaacggaaa ccctgacact tcaggaatcg tttatcgagt
      481 actcgtccat cctatgggcc aagcgattac atatctagtt gagaatttca attggtctta
      541 cggctgggca gttattgcaa tgactgtgat cgtccgtatc atcatcttgc cactaggtat
      601 cagtcaatca aaaaaaacaa tgatccaatc tgaaaaaatg caagcgttaa aaccacaagt
      661 tgaagcagca caacaaaaac taaaagctgc aacaacacgt gaagaacaaa tggcagcaca
      721 agcagaaatg caacaagtat atcgcgaaaa cggattgagt atgaccggcg gaatcggctg
      781 tctgcctcta ttgatccaaa tgccgatctt ctctgccttg tattttacgg ctcgctacac
      841 agaaggtatc cgtgaatcat ctttttacgg catcgatcta ggctcaccaa gtatggtgct
      901 tgttgccatt gcaggggtcg cttatctgct tcaaggatat atttcaacaa tcggtattcc
      961 tgaagaacag aaaaagacga tgcgtacgat gctgatcgtc tctcctgcta tgatcgtttt
     1021 catgtcgatc agtgcgccag ccggtgttac actttactgg gtagtgggtg gtgtgttcag
     1081 ttgtctgcaa acattcatca caaacgttat gatgaaacca cgtttgaaag cacaagtggc
     1141 agaagagttg aaacaaaatc cgccaaaaca agtcgtcact ccaagaaaag acgtgacaga
     1201 atcggcaaag ccagctgaca ataaacaacc aaaaaacagt acaaacaaca aaggccgcaa
     1261 cgcaggaaag cagcaaaagc ggaaataaaa atatatgaca aaaaactgta gacaaataaa
     1321 aaaatttctt tgttctacag tttttttgtt acaatcaaag aaaacgtcgc ttttcgagga
     1381 ggaatgtata atgatgtcca aataatcaat ggatagtcga atggaagaag ccaaaatttc
     1441 gctagatgca cttgttccta aaaatcattt ggtccataaa attgacaaaa tattgttatt
     1501 tgattttatt tatccaattg ttgaatcaac ttactcgacc attggtcgtc ctagcgttga
     1561 tcctgtcatc ttagtaaaat tagtattcat tcaatattta tttaggattc gttcgatgcg
     1621 tcagtccatt aaagaagctg aaacaaacct agcttatcgt tggtttcttg ggcttagttt
     1681 aatcgataaa attcctcatt tttcaacgtt tggtaaaaac tatgtacggc atttcagaga
     1741 aaccgattgt ttgaacagat atttatcatt atccttgaac aagcagttga agctagattt
     1801 attcatgaag aaattttgta tatggattca acctatatta aagcgaatgc gaaaaaacat
     1861 atttttacaa gagaaatggt acataaataa gccattttat accaaaaaca attgaaagaa
     1921 aaaattaatg aagcaagaat tgcagaaggc aaacgtcctt ttggttcaat cattcatcag
     1981 gaggtggtag aagggaaaat tagtgtcgca gatccagaaa gtggttatta tgtaaaaaca
     2041 gaacaagaaa aacagtttgc ttattctgct cataccgttt gtgatgaaaa tggttttgta
     2101 ttagatgtca tgattacgcc tggaaatcta catgacagcc gaatgctcgt gccacaaatc
     2161 gaacgtgtta aatcatgttg tggtgtagct gctgatgctg cgtacaaaac accttggaac
     2221 gccaagtatt taatcgatcg aaaactacga cccatttttc cttatactcg acccaaaaga
     2281 agcaaagaac gttttaaaaa gaaagatttt tattatgatc cctattataa ctggtaccta
     2341 tgcccaaatg atcaaacact tcagtttcgc acaactactc gagatggtta taaaaaatat
     2401 gtatctaaat cttttatttg tgaaagttgt ctattattga aaaattatac agaaagccag
     2461 aaacaccaga aagaaataca tcgacatatt taggaagact acttagatga agcagaacat
     2521 ttacgccata cggtgtacaa tcgcgaaaaa tataaaaaaa agaaaagaaa cgattgaacg
     2581 cgtttttaca gacgcaaagg aaaagcatgg aatgcgctac accaaatatc gaggcttgga
     2641 aaaaaccagg tgcatacgat gcttgttttt gccgcaatga atctgcaaaa attagccatt
     2701 tggcagtggg ggcatatg
//
LOCUS       DACCQI010000189         2715 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCQI010000189 DACCQI010000000
VERSION     DACCQI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.199"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1027)
                     /locus_tag="IVO76_002735"
     CDS             complement(<1..1027)
                     /locus_tag="IVO76_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7430659.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVL"
     gene            1826..2158
                     /locus_tag="IVO76_002736"
     CDS             1826..2158
                     /locus_tag="IVO76_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7430660.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT          853 a          477 c          510 g          875 t
ORIGIN      
        1 ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac aaaatttgaa
       61 tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt gaatcacaac
      121 catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca gaggctttaa
      181 agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc ttatcaaaaa
      241 tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag ttcttgcgaa
      301 cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg aacaaacctc
      361 gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg tcttgaacat
      421 caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata tatacacctt
      481 ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac atgaaaaatg
      541 gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga tccaactgtt
      601 tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc agttcaactg
      661 ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg gcagcgatca
      721 aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta ccgtctctgg
      781 ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga tcgtagtaac
      841 catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga atatattgat
      901 ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt gcggtatcat
      961 tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta aaatgtactt
     1021 gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg tactcctaac
     1081 tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg taatcgtttt
     1141 aaattcataa tcgttagata ttttagttat ttttcactca aaaaaatagg gaactagtgt
     1201 ttgcatatgt gcttttttga gttataataa ttcatgaaga gaggtaattc acttatttcc
     1261 ccaagctgaa aagcctgata ttacttcttt tcaggcagtg ctgctaatat tcaattacac
     1321 ttttttgtat atttttagtt gttttaaatt taattgtctg ctatgcttgt gatgaattga
     1381 ttttcgttct gcacttttgg acgataaatt tggacaattg aaaatacaaa aaaaagaaag
     1441 gccctgtgag actcggaagg ctggtaacct tcgtcgacgt ccacaggtgg cattaagatc
     1501 actcccttaa tgcgtttacc tttcagtgtt acggtattac ccgtaacgga tttagtataa
     1561 cagatattca aaagtgatac cacttttttt ggctgtgtta aggttaaaaa tacgtgatat
     1621 tacgatgaat ctatgcctaa gcataatgtt ataaaaatgt ccgtgtggct ctttgccgta
     1681 tgacgaacgc tgaaattaga gggaacgcat gtttggatgc gttccctctt ttttgtttgt
     1741 catgaacgaa aatcaattct ctatttttga aaaataacaa gaaaaacttg ttaaaaatat
     1801 ttaaagtaag tgggtggatt attttatgtc atatacagga attttgagtc tcgaagatat
     1861 ttgtcactac ggtaaacggt gtaccgctac agaaaagatc acgaagaaac tatcaactgg
     1921 tcaaagtaaa accgttgtgc agtgtaagaa atatataatc cagaaagaca aagtctcaga
     1981 agaaatgatt tattacattg ggaaacaaaa acaaataatt ttaaaagatc ctatcccttt
     2041 aaaagagcta tatccaacaa tcaaacatgt ttatgaccaa aacggtgtac tgattggtcg
     2101 aagaaaaaat ggagtcttgc gttgtaccgc aaaagggatg gggcgtttaa tcagctgaat
     2161 tgatcattct cataacttgt attttatcgt ttttcgcttg cttgaaaata ttgtcgagac
     2221 ttctaaagga acttagaatc actagaaaaa taaggagtag acttgatcta tcaacaattc
     2281 ctttcggggg gtgacggggt aggtttttac gattggtaac gtaaaaaatt ggtcaatcga
     2341 gtaaatgccc ttgtttggat agtggtgaaa tgaatttgtg taaataatat gcacagagta
     2401 aacaagaggg aatttagaag ttagacaagt tagaatagcg cctagattaa tcatgagcag
     2461 ctactaaggg atctaggctt tgacgcaaac tcatggaata aggagtaggt cttctcgtaa
     2521 tggggaggac gcacaagaaa ttgtgtcgtg tgctgcagta catgtagata cttgcgaaga
     2581 cgttgatctt tggatcaatc gagcatttac cgttttctac tagggtttct cgtttcggac
     2641 gccataaacg agtcgattta ttttatatat ttttgagtga acgaaaaacg ataaaaatat
     2701 tcgttatgaa gtaaa
//
LOCUS       DACCQI010000190         2636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCQI010000190 DACCQI010000000
VERSION     DACCQI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.200"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            474..1295
                     /locus_tag="IVO76_002737"
     CDS             474..1295
                     /locus_tag="IVO76_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7430661.1"
                     /translation="MYQFLKTGATILNYYQQYKENKKRCGRRSIALPEEQKEYVQKRV
                     VQGWTPDVIVGRSEMKLDCSVRTLYRMFQTGAFNVRDLPMKGKRKPNGHQEKRGKQAF
                     RRSIHERIKEHPEFENEFGHLEGDTIVGRKHKSAVITLVERLSKVIITLKPAGRQAMD
                     IENRINHWFQTVPKHLFKSITFDCGKEFSNWKAMGNQNDIDIYFADPGTPSQRALNEH
                     SNGLLRRDGLVKAMDFNEVSESFIEYRKHFLYSILSSFKFSIDYIYCEFCRGSET"
     gene            1382..1987
                     /locus_tag="IVO76_002738"
     CDS             1382..1987
                     /locus_tag="IVO76_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7430662.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            2003..2575
                     /locus_tag="IVO76_002739"
     CDS             2003..2575
                     /locus_tag="IVO76_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7430663.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLNDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
BASE COUNT          944 a          416 c          526 g          750 t
ORIGIN      
        1 gaataacaac tatgaaatat agagtatgta aatagcatgt ctataaaaaa taaagtagta
       61 aattaatcat aatcttttca acaacaggaa tagtcaagtt agactgttcc tgttgtttct
      121 ttattttcct tatcatcttc aactttttca aatactgcaa atacaaaata tcaatataac
      181 ttattcgttc tatcaatcag ttcctaatat ctactcatca aaatgaaatt tcaaactaat
      241 ctcatcatag tattcttttc gtcgattgta aaattaagct agacaactaa aaagatcatt
      301 tgtgctacac tcaaaatagt tccgaccaaa gaattattaa ggagtgagta caaatgacct
      361 acacccatct tacaacagac gaactggtaa tcatagagtc ttatttcaaa aataatcaat
      421 ccgttgccaa aactgcgcaa tgcttaggac gtgcgtgaca aacgattcat aacgtgtatc
      481 aatttttgaa aactggtgct acgatactga actactatca acaatacaaa gaaaacaaaa
      541 aacgctgcgg tagacgctcg attgctttac cagaggaaca aaaagaatat gttcaaaaga
      601 gggttgttca agggtggaca ccggatgtga ttgtcggccg ctcggaaatg aaacttgatt
      661 gttcagtccg tacactgtat cgaatgtttc aaactggcgc atttaatgtg cgtgatttgc
      721 ctatgaaagg caaacgaaaa ccaaatggac accaagaaaa acgtgggaaa caagcttttc
      781 ggcgcagtat acatgagcgc atcaaggagc atccagaatt tgaaaacgag tttggccatc
      841 ttgaaggaga cactattgtc ggtcgaaaac acaaaagtgc tgttattaca ttggtggaac
      901 gtttgtcaaa agtgattatt acattgaagc cagccggtag acaggcaatg gacattgaaa
      961 atcgaattaa tcattggttt caaacggttc ctaaacatct gtttaaatca attacgtttg
     1021 actgtgggaa agaattttca aattggaaag ctatgggaaa tcaaaatgat attgatattt
     1081 attttgcgga tccaggcacc ccgtcacaac gcgctctaaa tgaacattca aatggattat
     1141 tacgtcgtga tggattagtt aaagccatgg acttcaatga agtatcagaa tcatttatag
     1201 agtacagaaa acattttctg tactctattt tgtcttcttt taaattttca atagattata
     1261 tctattgtga attttgtaga gggtcagaaa cttaatagtt tttgacacat aaacaaaatc
     1321 gcgatataat ggggttgaga gtgtttcata aacttatgtc ataaagttaa ggagatttcg
     1381 catgatttta gagaataaat tgggattaac caatcaagtt gagttggcta aggttgaaga
     1441 aaaactcagt aaacaaaaag cgaaagaatt gtacgattgt ggaaaaataa atgaaatcga
     1501 agttggtaca tttaaaggtt tatctgaaat tcatgaattt ctattttcgg atatttacga
     1561 ttttgccgga aaaatccgat cggtcaatat tgcaaaagga aattttcgat ttgcaccagt
     1621 tatgtattta gaacattcgt tacagcatat tgatcagatg ccacaaactt cttttgaaga
     1681 gattatcaag aaatatgtgg agatgaatat cgctcaccca tttcgagaag gaaatggccg
     1741 aagtactaga atttggctgg atctcatttt aaaggaagaa ttgcaaaaag ttgtagattg
     1801 gaacttaatc gataaagcag actatctttc tgcaatggag cgaagtccca taaacgattt
     1861 ggaaatacgc tatctaattt ccaatgctct gactgataaa attgacgatc gcgagttata
     1921 tatgaaagga atcgatgtca gctatttcta tgaagggtat tctgagtata cgatagatga
     1981 tttgtaaaag taggtggacc aaatgacgaa atatggatat gcacgtgtct caaccgcttc
     2041 tcaaagtaca aaagaacaaa ttcaacagtt gcttgagaac ggcgtagaga agaagaatat
     2101 tttttcagaa aaatttacgg gtacaaaacg ggatcggcca aaattcaatc agctgttggc
     2161 tggtctgaag cctggagatg aaatagttgt tacgaagctt gatcgatttg ctcgtaacac
     2221 gagagaagca ttagagatca ttgaaccttt actcaatgat aacgtaacga tcaaggtttt
     2281 gaatctagga acgattgaaa atacgtccat ggggcgaatg gtgacacgga cgttgttgag
     2341 tgtagcagaa atggaacgtg atatgattgt ggaacgtacc caagaaggaa aaatgtttgc
     2401 aagaaagaat aatcctaatt tcagagaagg acgtccgaaa gcaacaatca caccgaagaa
     2461 gaggcacgca tatgaactgc ttacttcagg taaatcgtat aaggaagttg aagctataac
     2521 cggtttcagt cgcagtacgc tttttagaat aaagaagaaa attgaagaaa gttaaaaaag
     2581 aagtctcagc gaaccagaca ggtaaatact taatgcgttc tagatatgag tctatt
//
LOCUS       DACCQI010000191         2546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.201, whole genome shotgun sequence.
ACCESSION   DACCQI010000191 DACCQI010000000
VERSION     DACCQI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.201"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(82..1596)
                     /locus_tag="IVO76_002740"
     CDS             complement(82..1596)
                     /locus_tag="IVO76_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7430664.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            1734..2540
                     /locus_tag="IVO76_002741"
     CDS             1734..2540
                     /locus_tag="IVO76_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430665.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT          684 a          546 c          421 g          895 t
ORIGIN      
        1 actaaacagt gttcaatcag ctgaccctcg gtattagctc aaaacgctga tttcacaacc
       61 tctttttatt tacgttctat ttcattcatt ttccgaagca aatcgagctg cttgtatgcc
      121 cgcttgtcgc ccaaaaataa tgatctccgc aactgaattt ccaccgatcc ggttgtttcc
      181 atgtaagcca cctgtgactt caccagcagc atataacccc ctaattggct ggttttcttt
      241 atctaatact tcagtgttcg tattgatttt cacaccgccc atcgtgtaat ggattcctgg
      301 tgcgattttg atcgcataat aattcggaga cgataagtca tgttccatgg ctgtggcacg
      361 gttgaattga gcatcttttt tctccgcaac atccgtattc catccatcaa ctgtttttgc
      421 tagtgtttct tttggtaaat cgattttttc tgctaattct tccattgtct ttccagttgt
      481 gacatatcct ttttggtcat aaaattcaat agctgtggca cgatcacgca cgccttggtc
      541 aaaaatcaaa taagctgatt tttcaggcaa tgctgtaatt gctgcagata ctttatccct
      601 tgtatccatt tcattgacaa atcttttccc ctcttgatcg accaagattg ctccttctcc
      661 gcgaacaacc tcaccaatca agaccccctc atcctgctgc acagttggat gaatttgtat
      721 tttatccata tcaacggttt gtccacctaa tttttcaatc atggtgatac catctccagt
      781 tgatccttct tgatttgtcg taatataatc agctaaatcg ggacgatatt tttcaataaa
      841 ttctttgctg gcaccaaaac cgcctgtggt aactacgatt gcttttccgg tgacctcttt
      901 tggttcttct ccttgaacat gtacagtcac ttcattgacc gtttccccat ttttcttaat
      961 gtctgtcaca tctgcgttga caaaaatagg gatttcccgc tcatggacgt tattcaataa
     1021 cccctccact aagtatccac caatagccga gccatcagac ggacgatgtg tcctcttttc
     1081 actcatccct ccagtgatcg ttaagttgtc cagtttgata ccgttttgat ctagccaatc
     1141 gaccgcatca gcagagtgat cgacaaagta tcgaagcatt tctttatcat tcgttcctcc
     1201 accacctttt agcgtttctt catagaaggc atcattcgag tctttgattc cttcttcttt
     1261 ttgaaatttt gtttctgaag cattcatacc gcttgatgat ttagatgtgt ttcctccggc
     1321 aacaggcatc ttttccaaaa ttactggatt caacccagca tcttttgctt cgatagctgc
     1381 tgccatccct gcaccaccag ctccaataat cacgacatca taagattctt tcatttcttt
     1441 tggatctgta tatacttgtt cagaagcgcc tgatgatact tcggcagatt ccttcgtact
     1501 agaacttgtc tgcgctgtat ttttctgatc tgcgccgcat gcgctcatag caagaagtgc
     1561 aacggctgtt aataaaccag caactaattt tttcatttgt atcccccttt tctccttata
     1621 ctatattaag tgattttttt cacaatatca actctgtttt tctttaagtc atatagcttt
     1681 ggtaaacaca cgcttcttag tataattaag gatatacatg aaggagttat tttatgaatc
     1741 atttgaaaac cttttttacg aacagtacaa aaactgcagg tagaatcaca aacggaatct
     1801 atattttatt acgtatcatc gtcgttatta tgctagtgga tcaggtctgg aaccaacact
     1861 ggggaaatac aatgctttta ttactgacat taattttatt tgaaatccct tattttctgg
     1921 aaaagatatt aaagatacag attccaaata tgctagaaat cattttgtta tcttttattt
     1981 ttagttcaac agttttagga gagttaagtg atttttatgg ctatttttca atgtgggata
     2041 cttttctaca tgcattcagc ggatttttag ctgggggtgt cggcttttcc cttgtttatt
     2101 tgctcaataa aaatacaaaa accatgaact tgactccttt attactagca gttgtttcct
     2161 tctgtttttc gatgacagtt ggtgttatgt gggagtttgt ggaattttca gcagataaat
     2221 tactgcaatt cgatatgcaa aaagatacga tcatccagca aatcaatagt gtggagttaa
     2281 gtaaagacgg aaacactgtc tatcatgttc cagatatcac tcggacaatc attgaaagtc
     2341 aaaatgcaga tggtaaaaca gataaaacaa tcatccaaaa tggttactta gacgtgggaa
     2401 tcatggatac tatgaaggat ttatttgtta attttattgg agcagttgtt ttcagtgtat
     2461 ttggttacct ctatagtcga aacgattacc aaaaatttac gttcatcaag aattttattc
     2521 ctaaaaaaat caaacgataa aacatg
//
LOCUS       DACCQI010000192         2524 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCQI010000192 DACCQI010000000
VERSION     DACCQI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.202"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..451)
                     /locus_tag="IVO76_002742"
     CDS             complement(<1..451)
                     /locus_tag="IVO76_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7430666.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            675..1466
                     /locus_tag="IVO76_002743"
     CDS             675..1466
                     /locus_tag="IVO76_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7430667.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1432..2385
                     /locus_tag="IVO76_002744"
     CDS             1432..2385
                     /locus_tag="IVO76_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP7430668.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
BASE COUNT          887 a          418 c          402 g          817 t
ORIGIN      
        1 aatttgtaat aagttcgtat atgttttgcg gtgtttcttg tttcttgtaa ttttccacga
       61 atgtttcatc catgaataaa ttcataaacg cttgaagtaa tcctacttgt tcttttggtt
      121 ctttgattcc taaaaaaaat agattttcta cagtcatttc ctgattatcc cccatttgat
      181 aaaaataaat tgggttcttc agctgcataa caaaaacaaa tggcttttcg acatattcac
      241 attccgaatg tggcagcgcg atatttaaat acttagtaat caatccagta ggaaattctc
      301 tttctcttgc aatcagtcca ttcaaataat tctcattgac gtaccccatt ttttctaatt
      361 gttgtgcagc gattaaaaaa gcttcttctt tattttttac ttctaccgct aattgaatca
      421 aactaggaac gaacatctct ttatatttca ttccaacctc ctcctttttg attaagtttg
      481 ttaatttatt gttctactaa gttcatcttt gttttacaag aacattatat ttctgttagt
      541 ttgttttgtc aacgctttca aaagaaattt attttgttaa atttgatcta cttgaaaata
      601 aaatcgttta tctatataat taagaagaac aaagtcgaac aaaaattgac ttttatgtta
      661 aaggagtaat tcatatgctg aaaaaagaaa gacaagaaca aatcattaca ttattagaga
      721 aagattttta tttaactaat tcagaaatcg ctaacaaatt aggcgtatca gaaatgacca
      781 tacgccgaga tgtcactgaa ttagcaaaaa aaaataaagt aaaaaagcta tatggtggcg
      841 ttgaacgaaa ggaaccttat aatcgagaac tatcgactca agaaaagatt gatacgaatg
      901 tagccgaaaa aaaatatatt ggtcaagtca tgaacagtat gattcccgat aattctacta
      961 tttatgtcgg tgctggaaca acgatcctat atgcacttcc tttcattcaa aagaaaaatc
     1021 tatttgtaat tacaaacagt ttgattgctt tcaactattt aaagaatcat acaagttatc
     1081 ggattttact tactggtggc gaattttctc ctattacaga agagttcatt ggggaagttg
     1141 cagaaagatc ttttgaaagt ttgaatgtcg atatctcttt cgctgcaaca aacggcatat
     1201 ttgataacaa tgtgacaacc gctcaattta ttgagggaaa tattcagaac gcagcgctca
     1261 atgcagcaaa aatcaaatgc attgttgcag attattcaaa attaaataaa tcagacgtct
     1321 atacttttag aaaattaacc gattttgatt atctaataac cgataatcat ttatcagaac
     1381 aggatttcaa tcattattca aaatacttaa caatattaaa ggagcccaaa aatgatactg
     1441 acaatcacga tgaacccatc cattgattcc atttatttta caaaacactt tgaattaggt
     1501 acgatgacac gctttgattc tccggtacgc tatgtaggcg gcaagggaat caattgcgga
     1561 cgagcttctg ctctactagg ttcatcagtt attttaacag ggtttttggg cggttatttt
     1621 ggcagtatga ttcaagatat gctgaaaaat gaaaatttgt ttgatttgca ttttttacct
     1681 attgcagaag aatcaagagg tgccgtcacg atcatgcatg atgatgaaac gcagacagag
     1741 ctagtggaag ctggtcctga aatagcagat actactatca aaaaattaat gaatgatatc
     1801 aaactaatca gtaaaaataa ttacctttca gtcattacaa taaatggttc tgttaacaat
     1861 ccagatccct ttttgtatgc taaattatta caatatatca aagaacaaat agatccttcc
     1921 acctatatat taatggatat atctggacaa catctccgtg aagtcttagc aagtaagtat
     1981 aaaccgatgt tcatcaaacc taatatccat gagttttcag atatcatcca tcaaaaaatt
     2041 actcaaaaaa gcgacataaa acaccatcta aaaacaaatc cactatttaa agatatcgat
     2101 gttatcatga tttcttgcgg ctcgtatggt gcagtagtca aagtaaaaga agaattttgg
     2161 aatgttgaga tccctaagat tcctatcatt aatacaacag gctctgggga tgccactgtt
     2221 ggaggttttg ctcattcatt agaacagaat tatcctttaa aagaatgtgt aaggtacgca
     2281 atggcttgcg gcatgtcgaa tgcacagcaa tcagaagtcg ggaaagttat tccagctgat
     2341 gtaaaaaatt tagcaaaaag cattaaaatc acagaaatga aataaatagt acaaagtaaa
     2401 taattgttta tatgaaatga gacacaaaga gactgtgaca aaaaacgtgt gtaccgaccc
     2461 ccaaaagtta gattttttgg tctaactttt gggagtcgat acattgtcac agtccttttt
     2521 tcga
//
LOCUS       DACCQI010000193         2509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCQI010000193 DACCQI010000000
VERSION     DACCQI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.203"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(26..1573)
                     /locus_tag="IVO76_002745"
     CDS             complement(26..1573)
                     /locus_tag="IVO76_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7430669.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1675..2028)
                     /gene="tnpB"
                     /locus_tag="IVO76_002746"
     CDS             complement(1675..2028)
                     /gene="tnpB"
                     /locus_tag="IVO76_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7430670.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2018..2212)
                     /locus_tag="IVO76_002747"
     CDS             complement(2018..2212)
                     /locus_tag="IVO76_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430671.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          720 a          508 c          446 g          835 t
ORIGIN      
        1 atagaatcgg gaatgtttgg cgtttttagt tacgagaaaa atgttgttgt acatccttgt
       61 tccaaggcaa cagttgatct aaagtttctg ctgaaagtac gggaagattt ggtatcttat
      121 caaatagaaa ctctagatac tttacaggat tcaatccgtt aagttctgct gttttatata
      181 cactcagaat gattcctgaa gttcgtgctc cttcaagact tgtggaatgc aaccaatttt
      241 tccgtcctat caccagctct ttgatatttc gctccgcacg attatttgtg agttctaaac
      301 gaccatcttc aaaaataggt tcaaaaccac ttcgtagttt acacgcatat tgaacggctg
      361 catccaactt ggattttgca acaggatcta tcgttgccat ccactcataa aaagcaataa
      421 ataatggacg cagttcttgt tgccgacagc ttaatcgctt ctcaggagaa aagtctttcc
      481 atttcttttc taggacaaac catttatcca attgtttgac tgcttgagct gcgggaatat
      541 ccgtgttttt ctttcctttt ggaattgctt cgaaaaattt ccgccgaaca tgcgcgaaac
      601 aatagacaag tcgtacactt tccacggcat tatacccgct atagccgtca cagtgtaaat
      661 accctgtaaa ctccttgaga aaagttttcg gaacatctcc agcacgactt tcgtcgtggt
      721 gataataaac gatgggatgc tgactgtctt ttcctgtaca aaactgccag taataggtat
      781 ctgttttggc actttcaatc acacgataac ttgtttcatc cccgtgtaga atctcttctt
      841 gattaagata gactcttaat cgttctccta aagggtctaa cgcgttttgc actgctttaa
      901 tatgccaatt ggtgatatta tctctcgaaa tatccaaccc atgtgtttgt ttccaatagt
      961 tctcttgccg ataggcagga actttctgac caaatttcat ccggatcgtt tccgccacta
     1021 aactattgga acctaagcta ttagaaatca aaggtttagg aacgatcgct tttttaaaag
     1081 aagaaagtcc ttctttttcg cagcttttac aacaataggt atgttgataa tgggtgagac
     1141 aatccaacat agcctgatgg aaacgaattt cttctcgtac ttttgtccgt ccgatatctt
     1201 tcatctgtga accgcaatgt tcacaaaatt gttcttccaa aggaaggaga tgttctttct
     1261 ctgttatagg taagtgtttg attttgtctg ctttcgttcc tacaactcgt tgatgtcctt
     1321 ttactggaat aatggtggca gctgcttctg gaacacttac agaaatttct tcatcactaa
     1381 aaagattcaa ttgcccattc gtttcctcaa agagagtctc tttagagcgt ccaaataatt
     1441 tgttggttaa ataattgacc ttttcattca atagttggat ttcagttgta agattctgaa
     1501 tggtttgttg ttgtgcttga ttggttttat gggcttcttg aagcaactga agcaacgtat
     1561 ctgtagtttc caaaattttt caccaccttg aatcgaactg attttgttac agaataccac
     1621 aagaaacgca caagaaatag gggttcaaca cattttttgt gttaaccccc cagcttaaaa
     1681 aactcctttt tttcctggca aaatagctcg aggttgatcg atacttaatc cttctaacaa
     1741 ccatcgaacc tgttggttcg ttaaggttcg aacttcgtct ttcgtccgag gccattttaa
     1801 cttgccgttt tctatccgct tataacagag aagaaaacca tcaccgtccc aatagagaag
     1861 tttgaaacgg tcttgacggt tcccgcaaaa taaaaaaacg gcatcttcgt aaagctcgag
     1921 ttgatactct tgttgaataa gactggcaag accatcaatt ccttttcgca aatctgtttt
     1981 tccgcacaca atataaatat gtttggtttg aagtaaatca taaagcattg gacaacagtt
     2041 ctttcaaaat agcttgaaga atataattgt taataccgtt atagatcaaa acttcagaac
     2101 cattctctct tttgatgcgc atcgctaact tggcagccgc gggtttacct ttttctggtg
     2161 cactcttttc gtttcctaat tggacaggaa caatctcatt taatttatac atagaaaacc
     2221 tccagacttc ttgatagctc aagtatatct tgaggtgcat ttgttagtaa ggtactgttc
     2281 tatggggcgc ttacatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     2341 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     2401 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     2461 tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DACCQI010000194         2475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCQI010000194 DACCQI010000000
VERSION     DACCQI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.204"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            280..543
                     /locus_tag="IVO76_002748"
     CDS             280..543
                     /locus_tag="IVO76_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP7430672.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            537..887
                     /locus_tag="IVO76_002749"
     CDS             537..887
                     /locus_tag="IVO76_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7430673.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(911..1525)
                     /locus_tag="IVO76_002750"
     CDS             complement(911..1525)
                     /locus_tag="IVO76_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7430674.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            1839..2261
                     /locus_tag="IVO76_002751"
     CDS             1839..2261
                     /locus_tag="IVO76_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430675.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2271..2474
                     /locus_tag="IVO76_002752"
     CDS             2271..2474
                     /locus_tag="IVO76_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430676.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
BASE COUNT          872 a          364 c          419 g          820 t
ORIGIN      
        1 attttctatg tcctttttat tccattttgt atcaataact atgaaatctt taaagtatcc
       61 agaagagatt atttttagat caattttttt cttacctgat tctaaagatt ttgttttctt
      121 aaacatatta tgatttcctt tcgactattt tcatataaat gttaacatag tttatggatt
      181 catggaatgt taatctaatt taatagaaaa ggataattaa gtatatacgt tttttcttat
      241 gtatgttata ctatgtatat acagaaagag gtgtctacta tggaaatgtt acctataaaa
      301 aaatggggaa atagtaatgg ccttagattg ccaaagtata ttatggaata tcttggaatt
      361 catacagaag acaaagtgaa gataatacaa gaagaagtaa acggtcagaa gcggctaatt
      421 atcgaagcgg ctaatataga aaatgaatta acaattgaac agttatttga aaattacact
      481 gatgaaagaa atcatgtgac gattcaaaat ttaggtgaag cagtaggtaa tgaaaaatgg
      541 tgagaaaacc tagacaaggc gatattctat tattgaatac agcacctcga tctggacacg
      601 aacagacagg taaaagacct tatattgttt taagtcatga tattattgcg gattatagta
      661 acgttgttac agttgctcca atttctagta caactagaaa ctatccgcta tatgtaaata
      721 taaactcaga atatgaaatg aagacaacag gaaaagtatt attagatcaa ctaacgacaa
      781 ttgattatga agccagagag tgtatttttt tggaaaaagc gcatgatagt ttagttgagg
      841 agttattgat gaaagttaga acagtttttc aaaaagtaag caaataaaat agaaacagca
      901 gcatgatcaa ctattcgtga ttgaccatgc tgtctttttt tattctatat acggtttgtc
      961 tcgtaacatt gtagtctttt gcaatcttag cgatcgcaac accttttttt agatcctcaa
     1021 cgatgttttt ataaacaaga cgacgttggg gatcttttgc attggcactg tatagcttag
     1081 gacgtccttt ataaacaccg ttcgcttttg caatcttaat gccttgtgcc tgacgtcgtt
     1141 tagattcggt acgttcttgt tcagcgatca tggccaaaat ttgaatgatc aaatctttaa
     1201 tgaatttatc caggagagga ttgccaattg cttcggccat gatcggtaga cttgtgatga
     1261 tcagctgcac atttttcttc ttcaagtaat tgactgaagc gatgatctcg tcgtaatttc
     1321 ttccaagtcg atcaatagct tccacaataa aaatatcttg atctcggacg aaatccagtg
     1381 cttcttgaaa aacaggtcga tgagtaatcg ttgcgccaga ttgtttctca atgaaaattc
     1441 tctctgcacc gaattttttc atttcttcaa tctgccgttg ttcattttga tcgatggatg
     1501 agacacgtat gtatgcaact ctcaaaattt ttacctactt tctgcaacta tttaaaaaac
     1561 accctaatca tacacgagaa aaccagattt ttcaatgtcg cggtcgtgtc tagttagggt
     1621 ataccttata tagacaaaaa ctaatgatgg aggaaatagt atgtgtcact tttcttttta
     1681 aatggcaata ttttttgtaa ataaatgtca atcaagaaaa tttaaacgat tatgacatta
     1741 aaaatatatt ttatgaattt tctagtatat aacttgattt atctgttaag atttattaag
     1801 aaaacgtttt cttatttttg atttcgaggt gatagacaat gaaaaataag tttattttat
     1861 ctttaggtat tttaactatt ggaattagtg taactggaac tacaattcct acatttgctg
     1921 atagtacaaa tgaaccaact actgtagtag ataataacca agaattaact cctttagata
     1981 ggaacggaat agccattggt aacccaacta acgacttaac tgttactgaa aattatttaa
     2041 tagtttctcc attacttgct cgagcagctt ctaaaacgat acgagtgaca ggaacgatga
     2101 tttttgataa agttggttgg ccttctagac ctcctcaatc acggaattgg aaagaaaaaa
     2161 gaaatggtta ttggtactat ggaaaattgt atttacaaag ttataaatca gctggaaaca
     2221 attggattgc gacttattca ggaaccttaa ctagaaagta gggatttttt atgccagaat
     2281 ttgtgaacac accaaatttt tatcaagata gcgatattct aagtaaaatt aatttaccga
     2341 aagaagaatc agctttacaa gttcagcgaa ctttacagga gacacaaaaa attcaaaaat
     2401 caatttctaa tctagaagat tctgaagaaa aaagaaaaaa aagagaccaa aaaacatatc
     2461 aaaaagaaaa ataat
//
LOCUS       DACCQI010000195         2426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCQI010000195 DACCQI010000000
VERSION     DACCQI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.205"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            337..1917
                     /locus_tag="IVO76_002753"
     CDS             337..1917
                     /locus_tag="IVO76_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7430677.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            2115..2339
                     /locus_tag="IVO76_002754"
     CDS             2115..2339
                     /locus_tag="IVO76_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7430678.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
BASE COUNT          877 a          388 c          510 g          651 t
ORIGIN      
        1 cagctgacaa aaaaatattt gactcttatg gtttggtggc tttttcattc gatgaatcca
       61 tgctatactg attcagttgt agtagttgac aaaggttgtg aaaaagctat cctgcattaa
      121 gtaataagag cagccaagca aaaacagctt ttcgtatttt tggcttgggt gggcttattg
      181 ctgataggct gtctctagaa cgccgtttac tcggaaaaag agactgctac acggcttttg
      241 tcacagccct atatgaaatt gtctttcttt aagagatcgg acaatctgtg cctagagaaa
      301 atataaataa ttgaatggaa gaaatggagt taggttatga ataatccaga attaaagcaa
      361 caagtggaca atcgcagaac ttttgcgatc atttcccatc cggatgctgg gaaaacaacg
      421 attacagaac aattactatt gtttggtggg gctatccgtc aagccgggac cgtaaaaggc
      481 aaaaaaacag gtaattttgc caaatcagac tggatggaaa tcgagaaaca acgtgggatt
      541 tctgttacta gttctgttat gcagtttgat tatcaaggga aacgagtaaa tatcttggat
      601 acaccaggac atgaagattt ttctgaagat acttatcgta ctttgatggc cgtggatagt
      661 gcggtcatgg taatcgatag tgcaaaaggg atcgaagcac agacaaagaa attattccaa
      721 gttgtaaaaa aacgtggcat cccgatattt acttttatca acaaactaga tcgtgatggt
      781 cgtgaaccat tggaattgtt agaagaatta gaagagctat tagatatcga atcttatccg
      841 atgaattggc caatcggtat gggtaaaggg ctagaaggtc tttatgatat ctaccataat
      901 cgtgtagagt tttatcgtcc ggaaaattat caggatgaac gattagtaga attggatgaa
      961 gatggactgc ttcccgaaaa tcatcctcta actgaaaatt ccttatatga acaagtctta
     1021 gaagaagtag aactaatcaa agaggctgga gatgcattta atcaagaaaa aattgctcgt
     1081 ggcgatcaga caccagtatt ttttggttct gcattgacta acttcggtgt acagactttt
     1141 ttagagactt tcgttgaatt ggcaccagct ccttatggac acaaaacaca agatgatcag
     1201 atggtaagtc cttatgagga agaattttca ggtttcgtat tcaagatcca agcaaatatg
     1261 aatccagccc atcgtgaccg aatcgctttt gtccgaatct gttctggaac attcgaacga
     1321 ggaatggatg tctggttgga acgtacaaat aaaaaactga aattaagcaa tgtcacacaa
     1381 tttatggcag attcgagaga aaacgtcgaa aaagcagtag caggcgatat catcggtgta
     1441 tatgatacag gcaattatca gattggggat acattgttcg aaggaaaact aaaagttgct
     1501 tatgaagaac tcccttcatt tactccagag ctgtttatga aagtgactgc aaaaaatgtc
     1561 atgaaacaaa aatccttcca caaaggaatc tatcaattag ttcaagaagg agcgattcag
     1621 ctttataaaa catatctaac ggaagaatat atcattggag ctgttggtca gctacaattc
     1681 gaagtattcc aatatcgtat gttaaatgag tataacgccg aagtaatcat gtctccaatg
     1741 ggacacaaaa tcgctcgctg gatcgatcca gaagatttgg atgagaagat gagttcaagt
     1801 cgcaatatct tggctcgtga ccgtttcgac caaccattgt tcttattcga gaaccaattt
     1861 gcagaacgct ggtttgcgga taaatatcct aatgtaaaat taaaaagttt gatgtaaaga
     1921 agtcttgaca caagctgccc tgcatcaagt aataagaata gccaaacaaa aacagcctct
     1981 catattttgt ttagagccgt gacggatttt ccgtgacggc tctttttact atattaaaat
     2041 catagaaatg tctaatcaaa aaaatataaa aattattttg cgtacatatg attaaagaaa
     2101 ggagagtgtg acacatggaa acagaatggc aaatacgtga aagaaaaaaa cagaaaaaag
     2161 aagagatgtt acgattatgt cgattattag atgtaaaaga ctgggacaaa aacgagcaaa
     2221 ttgcacaaaa agtacgatca atcatccata aagaggaaaa agttaaaaat aaagacacgc
     2281 gtagaactga aaatgaaaag agaaaagtac aaaagaaaag agaaaaatca aaaagctaga
     2341 aagaaaaaga ggctgagaca gaagcgttta actccaagaa ataagctgga attcacaaaa
     2401 atttgaaaag caattttcgt gaattt
//
LOCUS       DACCQI010000196         2399 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCQI010000196 DACCQI010000000
VERSION     DACCQI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.206"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IVO76_002755"
     CDS             207..2069
                     /locus_tag="IVO76_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7430679.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2399
                     /locus_tag="IVO76_002756"
     CDS             2069..>2399
                     /locus_tag="IVO76_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7430680.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatt
//
LOCUS       DACCQI010000197         2370 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCQI010000197 DACCQI010000000
VERSION     DACCQI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.207"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            54..656
                     /locus_tag="IVO76_002757"
     CDS             54..656
                     /locus_tag="IVO76_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430681.1"
                     /translation="MLKLISYAKKANSLSLVDNLDNLLPDKLIYDENEDNSQKQIIIF
                     ELYLKWLSSLSVSPESNDDDKYFANKLFQFIFPDYSSMPFFTLINFMLYVKEHLGYDD
                     LVEMIKAYDKEPYPFKGINTGSDMAMISGETVEEILANYKEQNIKTLELIKKNIKYYD
                     PLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKELF"
     gene            complement(653..1276)
                     /locus_tag="IVO76_002758"
     CDS             complement(653..1276)
                     /locus_tag="IVO76_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7430682.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            1594..1932
                     /locus_tag="IVO76_002759"
     CDS             1594..1932
                     /locus_tag="IVO76_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7430683.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            1932..2370
                     /locus_tag="IVO76_002760"
                     /pseudo
     CDS             1932..2370
                     /locus_tag="IVO76_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT          860 a          348 c          399 g          763 t
ORIGIN      
        1 aatttttaga agaattatct ggagaagaat atagattatt cctaaataat aatattttaa
       61 aattaatatc ttatgccaag aaagctaata gcctatcttt agtagataac ttagataatc
      121 ttttacccga taaacttatt tatgatgaga atgaagataa ttcacaaaaa caaataatta
      181 tatttgaatt gtatttaaag tggcttagca gcctgtcagt gtcaccagaa agcaatgatg
      241 atgataaata ttttgcgaac aaactgtttc aatttatatt tccagactat tcttcaatgc
      301 cattttttac attaataaat tttatgctgt atgttaaaga acacttagga tatgatgatt
      361 tagtagaaat gataaaggca tatgataaag aaccttatcc attcaaagga atcaatactg
      421 gttcagatat ggctatgatt tcaggcgaaa ctgtagaaga aattctagct aattacaaag
      481 aacaaaatat aaaaactctg gaattaatta aaaagaatat aaaatactat gatcctttac
      541 tagcgaataa agaaaatatt gataagttat taaatgcaat tgctgattct gatgttagat
      601 ctcctagaat tgatgagtta aaagaagaaa ttttaatgta taaagaactt ttttaattca
      661 tttgattact gttatactca atatcattct taattctgta aatagtttgt ctagtaatat
      721 tgtagttttt agcaattttt gaaatagcta tgccctcatt taaatcctta acgatgtttt
      781 cataaactaa acgtcgttga ggattttttg tatctgcact atagagtttt ggacgacctt
      841 tatagacgcc atttgctttt gcaattttaa tgacttgcgc ctggcgtcgt ttagattcgg
      901 tacgctcttg ctctgcaatc atggccaaaa tttggatgat taaatcttta atgaatttat
      961 ccaaatgagg attgccaatt gcttcagcca tgatcggtag actggtgatg atcagctgca
     1021 cgtttttctt ctttaggtaa ttgactgaag caatgatttc gtcgtaattt cttccaagtc
     1081 ggtcgattgc ttcgacaata aaaatatctt ggtctcggac aaactctaaa gcttcctgga
     1141 aaatcggtcg atgagtaatc gttgctccag attgtttctc gatgaaaatt ttttctgcgc
     1201 cgaacttttt catttcttca atttggcgtt gctcattttg atcaatcgac gaaactcgaa
     1261 tataagcaat tttcaaaatt tttacctact tcctcaaact atttaaaata cactcaaaat
     1321 caacactaat ttttttatac actataaagc tgtcattttt ctagataaat ggcataaatt
     1381 ttcagcgcta agtgacaaaa gcaactaatt tttcatgata gatttttaaa tatctcaatc
     1441 tatctccaaa attagttgct tttgtagcta tagttgacta tgaatatgac aaataattag
     1501 aaaaaactgt gccaattaaa tatcaaaatg acaaattgat gaatcaaatt caaattaagg
     1561 ggggcaaccc tggaagtctt aaatttaccc tcaatgaatg gtattgaaga tgaaaattta
     1621 aggcgtttga ttaatagcct tgtcattgaa ttgtacaagt atcaagcaga atcagaacga
     1681 aaaaaaatta aggaacgtca ggcacaagga atcgaaattg ctaagaaaaa aggcaaattc
     1741 aaaggtcggc aactgaaatt taaaaaaaat gatccacgtt taaagcatgc tttcgatttg
     1801 tttttgaatg gtttatccga taaagaagtt gaagaacaaa ctgggatcaa tcgccgaacg
     1861 tttagaaggt atcgagcaag atataacgtg acagtagatc aaagaaaaaa caatgaaaag
     1921 agggatagtt aatgagtacg gttattttag ctgaaaaaca agccaggcat tagcctacgc
     1981 aagtgcttta aaacaaagca ccaaaaaaga cggttatttt gagatcaaag acccactatt
     2041 tacagatgaa acgtttatca cctttgattt tgggcattta gtggaattag cagaaccagg
     2101 tcattatgac gaaaagtggc aaaattggaa acttgaatct ttgccgattt ttcctgatcg
     2161 atacgatttt gaagttgcaa aagataaggg aaagcagttt aaaattgttg cagaacttct
     2221 caaaaaggca aatacaatta ttgttgcaac agatagcgac agagaaggtg aaaatatcgc
     2281 ctggtcgatt atccataaag caaatgcctt ttcaaaagat aaaacattta aaagactatg
     2341 gatcaatagc ttagaaaaag atgtgatccg
//
LOCUS       DACCQI010000198         2285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCQI010000198 DACCQI010000000
VERSION     DACCQI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.208"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(641..1255)
                     /locus_tag="IVO76_002761"
     CDS             complement(641..1255)
                     /locus_tag="IVO76_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7430684.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1861..2238)
                     /locus_tag="IVO76_002762"
     CDS             complement(1861..2238)
                     /locus_tag="IVO76_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430685.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          752 a          432 c          331 g          770 t
ORIGIN      
        1 ttctcctatg tatgattatt tttttctctt tccatttata ttaagtattt tatttctctg
       61 tttttcctta aaattctttt tttattttta gattagagaa aatctcgtcc tgtttttttc
      121 attttacaat cattgaaaaa ttgaatttca ttgcaaaaaa aataataaac acttataaat
      181 atccattatc tatccttcaa tttttgtgta ttttatttct attagaaaaa ggtatcctgt
      241 tggacaattt tgttatatca ggtaaacttc attgcaataa tgataacaaa tgtactataa
      301 ttactttgta aagagttttg gggaagactc tttcatcaac gtgtattaag agacaagaaa
      361 gaatccatat ggattctttc ttgtctcttt ttccactact ttttattttt ctgacactac
      421 atattcttat aactcatttc tcataactgt gaaacaactt ataaaaataa caaactttaa
      481 acaaaacaag cttaaaatca aagatgatcc atcttaaaat aactaaaaca aaaaaagtag
      541 tcgttgttaa tcctcattaa taatcgtttt ctttttttca aacgctccat ttttatgtgg
      601 aacattattt ttttaaggaa ctttgtcaac atgtggataa ctattttcca cttgtgtcct
      661 ggactagctg ttttgctgta aaatacaaaa cttgctgcag caaaaagatc gttgcaagag
      721 aggctgttcc gcagatcgct ggatctagat ttggcaaagt caagactgca tcatgtaaat
      781 tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa tcgcaaacca ctgctatgtt
      841 gcgatttgat acttcgtgac aaatcaaatc cagtaacaat gattaatgga tgaccatttt
      901 ctttgatcca ctgagctagt tcgatgattg ccgggtttct tccttcattc gaaagaagaa
      961 gaaaaatgtc ttctgatttg acagaaagct gatcaataat tgcctgtcct gcaccttcta
     1021 gcgtttcata gacacctccg gctggatttt caatcaatat acttggtaca agatccgggg
     1081 tttgatccca aaattcaaat gctacaccac ttagtgtgcg gctagcatat atctgcaatc
     1141 taccaccttg attaatttgt ttcgcgatcc aagtgccagc ttgaagcatc agcggctctt
     1201 ctatccttaa tttctcttcc aattggtgat aatagtcaaa ataacataaa ctcatttatc
     1261 tctcccgcct agctatactt gacatgaata gaaacagctg cctaatctaa ggaagtacat
     1321 aaaaaaacag tagttagaca gtaacacatg tggtctttgc tgtttcaaac acaaaaaagg
     1381 cattgaacga atacgcccaa taccttgaca tagcaaataa aacaacgtgc agaccatgga
     1441 aaatatctac ataggggtag aattttcacc ctagattagg tacaataaat taggtcgatg
     1501 cactgcattg actatggcaa aatgatcggt cagggtgtat gtgtgtgact ggtcattccc
     1561 tgattcttag cgccaactaa gggtcaggga gccttttttt attcagctgt ttctatctga
     1621 atttattgta agtaatctct tatccaagtg caagcaagaa aatacaaaaa aacattggta
     1681 tgatttatat atatataaat aacataaaaa aaggaatggc ttatcccgat acaacaagca
     1741 ttcctttaaa aaagattctt ttatataagt gaaaaaagct tttttccacc aattaaataa
     1801 atctctcatc tcaatagttt atccattatt ttatcttttt ttgcgtatac ctactaaata
     1861 ttaccgtctt cttctcgttc ttctactttg ttcgagagaa tctgtactag aaaaagtagt
     1921 tgtatgtcta ccttttctgc ctatccgaga ctttcttttc atatatggga tataaatgat
     1981 tagatacagt acagcaaaaa ataaagcaaa tacgcaagct tcccaactac ctaacgccaa
     2041 ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac cagataaaaa tcagaaacgt
     2101 ttcttttttt ctgaccatgc gaaacatgat aaatagacta ataagcaata gtggcaacac
     2161 aaatatccaa ttgcttcctt ccatcagcca cttgagtata tcaagcagcg actgtgcaac
     2221 agcgactaat tttcccatag tgaacacaca ccttttttta taattaaaaa attcgccttt
     2281 ttatt
//
LOCUS       DACCQI010000199         2275 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCQI010000199 DACCQI010000000
VERSION     DACCQI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.209"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(69..515)
                     /locus_tag="IVO76_002763"
     CDS             complement(69..515)
                     /locus_tag="IVO76_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7430686.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(542..718)
                     /locus_tag="IVO76_002764"
     CDS             complement(542..718)
                     /locus_tag="IVO76_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7430687.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(880..1308)
                     /locus_tag="IVO76_002765"
     CDS             complement(880..1308)
                     /locus_tag="IVO76_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7430688.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1400..2260
                     /locus_tag="IVO76_002766"
     CDS             1400..2260
                     /locus_tag="IVO76_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7430689.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLKQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT          673 a          454 c          369 g          779 t
ORIGIN      
        1 tttttagttc tttttttacg aacacaaaaa actgcggaca atcactgtcc gcagtttaaa
       61 tgataaaatt aagattgtaa tagttctttc acaatcgcat tcacttggtt tccatctgct
      121 tttcctttga ctttaggcat aattgcaccc ataactttgc caaattcttt cattgatgaa
      181 gcaccagttt gagtgattgc ttcttgaaca agttgtcgaa tttcttcatc ggaaagttgt
      241 tccggcatat atttttcgac tattacaatt tcacttttta ctttttcagc taaatcgtca
      301 cgtcctgctt cttcaaactc atgcaaagaa tcacgtcgct gtttcatttc gcgagacaac
      361 accgttagtt cttcttcttc tgtcaagtcg tgaccttctt taatctgctc gttttgtata
      421 gatgacttga tcattcggat gacttgtaaa gattccttat ctttcgcttt catcgctgct
      481 ttcatgtcat cgtttaatct acttagtagt gacacactac caactccaat tcatcacaga
      541 actagaattt tttgcgtttt ctagctgctt cagatttttt cttgcgtctt acacttggtt
      601 tttcgtagaa ttcacgttta cgagattctt gtaaagtacc cgcttttgac acggaacgtt
      661 tgaagcgacg aagagcatcg tcaagtgatt cgtttttgcg aacgactgtt tttgacatgt
      721 taattccctc cctccgagct taaaaagtaa ccgtaattgt tattgaaata tatctcaaaa
      781 aacaagactg tcctgtaaat attatatcta atgctgtttt gaccgtcaag gaaatcttgc
      841 ttttttttaa aattatcaaa tgttatttta tttaaaccct tattgacaat tttcacatct
      901 tccgaaaatc tcgaatctat gcccttcgat cgtgcatcca cctagctgtt cttcgaaata
      961 attcatcgga cacatatgga tttctttcgt tgttccgcac actgtacaga taaaatgatg
     1021 gtgatggccg atttccccct gacagcaatg aaaacgaaac ttcatttctc catttaaatc
     1081 ggtttcttcc agtaaatcga tctctgcaaa atcatggaga ttccgataaa tcgtatcata
     1141 acttacccct ttgaattcct gattcatata gtcaaaaact tctttagcag atacgtagcg
     1201 gttacgcttc actaaataag ttaacaatgc ttctcttttt ttagtatatt taaaaccgtt
     1261 atttttcaat gtttctaatg attgttcgat caatgtctgt tttttcatga cttattcctc
     1321 caaatcaaaa tgattacgaa gtacgcattc tagagtatgg tataataaac ataaaaaaaa
     1381 tgcaagtgag gttttatcca tgtcttccga ttctacagaa atcgttactt tttttgtcat
     1441 ctccgactct gctggagaaa ctgcgacaaa actagcgcaa gctactatgg ctcagtatcc
     1501 taccgttgag ttcaacctat tccgcagaac gttcgtgaca gatgaaaaaa cattaaagca
     1561 ggcattgcaa gatgcattag aagaaaaagc acttgtcctg catacattaa tcaatcaaga
     1621 actgatcgag ataacacgtt ctttttgtga agaacatcaa ttatttagtt ttgatgtgat
     1681 gacgccaccg atatcagaaa ttgaacgttt aaccggtatc aagccaattc ggcaacctgg
     1741 tgccctccat ttattgaatg agaactattt caaacggatc aaagcaatgg aatttgctgt
     1801 aaaatacgat gatgggaaag atcctcgtgg ttttttagaa gcagatgtcg ttttgctagg
     1861 cgtatcccga acttccaaaa cgcctttgag cttgttttta gctaacaaaa atttaaaagt
     1921 tgccaattta cctttgatcc cacaagcaca tattcccaaa cagctttggg agatcgatcc
     1981 taaaaaaatc gtgggactaa cgaataaccc agatatttta aataatatcc gtaaagaacg
     2041 gatgcgctct tacggattaa accctgatac tgcttattcc gatatcgaaa aaattagagc
     2101 tgaactcgat tttgcgaatg atctttatga aaaactagga tgtgtagtga tcaatgtggc
     2161 atctttatct attgaagaaa cggcttcgct tattttgaat gctttagacc tagaagatca
     2221 tagttattat ggaactgaaa caacagaaaa aagcgattaa tcatttttgt ctttt
//
LOCUS       DACCQI010000200         2271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCQI010000200 DACCQI010000000
VERSION     DACCQI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.210"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            192..446
                     /locus_tag="IVO76_002767"
     CDS             192..446
                     /locus_tag="IVO76_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7430690.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            447..764
                     /locus_tag="IVO76_002768"
     CDS             447..764
                     /locus_tag="IVO76_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7430691.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1323..2258
                     /locus_tag="IVO76_002769"
     CDS             1323..2258
                     /locus_tag="IVO76_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430692.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          430 g          711 t
ORIGIN      
        1 ggatgttaac ggaagtgtat ggtgagtagt tcactcgtat tttgatatcg tttgtatatg
       61 ttttcagcgt tctaagtggc ttattttgtc caataataat atggagtgga atcccttaag
      121 aaacaccgtt ggcaattgtt gttataacat gttataattt attcatcaaa aactaaaagg
      181 agtgtgataa catgggaaaa gaactaaagg ttagaaagat cggcaatagc gtgggagtga
      241 tccttccaag ctctcttgga ttaaaaagcg gagacattat ccaagccaaa caagaaggca
      301 acttattcat attagatact actcaaatag caaaagaaca cgatcgcaaa ttgattgaag
      361 aaagtttcca agactttgag aaaggtttaa cagtttcaga aattgaaatg gtaaaagctt
      421 tcggaaaata cggctggagt gaatagatga aagagtacac gctagtttat tctaaaagtt
      481 ttcagaccag ccttgaaacg ataatagaag aatgggaaaa tgagcttgct ctttctccag
      541 ccagcataaa aagatttgta ggtgcgatcc agaaggcttt agaactaacc aaaaattttc
      601 caactatgca tgaggaagtt tccgaaatct atggaatgga tatcccaaca tatcggatcg
      661 tgatcggcaa aagctatgcc ttattttatc ggatcgacca tgaaaaaaaa gagatacttg
      721 taggacgtat tttccaatct aaacaaatga aactaggctt ttaacaataa ttaggaaaaa
      781 gtcgctcatc ctctacaggt gggtgattaa tgccgctcga tatggttgtt aaccgtcggt
      841 tactcgaaga ttgatatata agacttagta cctttttatt ttgctccatg ttatggccga
      901 aggtctaaag atacaatcat tagtctagag aaaaattaag aaccgtagga actacgggga
      961 tcgcttggtg aataagagac actgctatta gtgaaaaaat aagatatcaa gtacgctcta
     1021 ttcccaaaaa tttcgtgact ttactcatga gaggttcaag tagtatattt ttaatcaaaa
     1081 ttgacgtaac ttttaataaa tactacctaa ataaggtagt atttattttt gtgttttaaa
     1141 agaggaagtc caatatatct atgggaaact tcttaaatat tatgttggta catgaaaata
     1201 tacatccctg ctagactatt tacaacattt tactttgtac gtattcttgt tttaaaagga
     1261 atgggataaa atataggtac aataactatt tgatgctaaa tataattaat ggaggagaga
     1321 tagtgaacat acttaatgaa aaaagatatg tatggtttgc tggtaaaaaa tatattacta
     1381 ctttcaaaag attatccgag ttaatgcagc agactgttaa aagtgctatg cctgaaggat
     1441 acaagtttaa ttttgttcaa tcttcagtat ctactgaaag tatctatttt tcaattcttt
     1501 ctgagagtag gctttttcat tttagaataa gttctcatgt aaagaaaact gatgttgata
     1561 tatatacgtt ccgtacaaat aattataaaa atttaaatga tttgagtaga agtatcagtg
     1621 aatatctcaa ggggaatagc tatctcaaga ttcgattttc acatatatta attttactac
     1681 catatttgaa tgagaatttc accctaattc aagtagataa gtctttcatt gcctcaggta
     1741 atcaaggaaa tatgaatatt acaagtaaca aaacgtcacg tgtgctattg gcgttgatgg
     1801 gttacggaat tctttataga gatgaccaaa atggccagat tctaataaat cgggctgggc
     1861 gttcattatt aatgaaatat agaaaattta taccggaaga ttttcaaaac ttacagttag
     1921 aaaacatgcg tttagaagat ttatttagat tagcagaaga tgataagtat agatttgaag
     1981 ttttgaaaat tcaaaagaat gaactattga ataagttata tcctgagaat gcgataaaag
     2041 cacattttgg tgatgctaat catatgtata gttggttcat tcctaaaaga tttagaggga
     2101 aaatcaatgc tggagatcaa gtgaaagtac tcaatcgaaa tagtacagat gtcgtaatga
     2161 taacagatat ctatgtagaa gatgaagata taatcaaata tatgaagcct gtgataaaac
     2221 ttgttaataa aaataaagga acccgtggtg ctagttaatt ttttcctaga g
//
LOCUS       DACCQI010000201         2152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCQI010000201 DACCQI010000000
VERSION     DACCQI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.211"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(682..972)
                     /locus_tag="IVO76_002770"
     CDS             complement(682..972)
                     /locus_tag="IVO76_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430693.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1180..1872)
                     /locus_tag="IVO76_002771"
     CDS             complement(1180..1872)
                     /locus_tag="IVO76_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7430694.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          619 a          397 c          313 g          823 t
ORIGIN      
        1 tataacatag ctttaagcat tacttaaaga taacttaaaa gttatctttt aaatacttaa
       61 ttgttaaata ccttatttga tgcaaaagaa atctacgata gtcaacaaaa aaattttcat
      121 tatatccaga aaaatttttc ttagttaagg acctatggat tgcagtaatg cagaattcgt
      181 cgaataaact gccaaagaca tacaatcata ttgcttttaa aattgacgaa caagatatag
      241 attcgtttgt ctcaaaaatc caaatgttag gtttaactgt cgaaccagga cgttctcgtg
      301 tgaaaggcga ggcttcttca atttactttt acgactatga taatcacctc ttcgagttac
      361 ataccggtac tctccaggaa cgtttaaaat cctacaattc cactacctga gactagtaat
      421 gactagtctc tttttatggc ctctgatccc cccattgagc gaatactaat agggcttcga
      481 attgatctgt tcaatcttta agcaatatcc ctcaaatgag tttggaaagt ttagtgattc
      541 atcacaaata cttaaataca tgtagttata tatagattga ggttcttgtg agcgtatgct
      601 cgtgtgggtt caaatcccat cgtctgcatc catctttcca aaacaagcgg ctaggacatt
      661 tttgtctcag ccgcttgttt tctagtttat tctttcatct tgtcggacct tttttcttgg
      721 gtatagactt ttctcatatt ctaccacaat tttgggtttc cctaacacat gataggatct
      781 cacaacaaat tgctttctat tattataata acctgtaacg actaacttca tcccttcgtc
      841 tacttcagct aggaagttca aactatggga agcaatcaaa caattgaccc catttaaact
      901 gaaacggatc aacggtctgg cactaacttg caaagtacgg atcttatcta ctgttccata
      961 ttttttctcc ataaccttca cctcaatacc attatacgaa catacattcc ctttgtaaag
     1021 aagaaactct gttttatctt tttgactttt tgtctattac ctttcttaaa atcatattgg
     1081 cacttcctgc gaagatgaag gacatgtact aaaaaaaaga ggcagcagta tagattactg
     1141 ttgcttcttt actaatttag gtttacttgt taagaaagat tatttacgca aatgttcttc
     1201 aacaatttgc tgagatttat cagctgtatc ttccacttca tcttttgctt tttctaactt
     1261 ttgaatatat tgtttcaagc ttgagttttc agcagttgct tcgtctaatt tcttttgtgt
     1321 atctgttaac tgagtttcta catttgtttt ttcttgtgtc agtgttgcga tttgttgatt
     1381 caaaccattt acagtatttt gtaattctga aagttttgct tgtgcttctt gtagtcttgc
     1441 tgtccaatct gcattcagat gattgatttc atcatttttt tggttgatct catctagttt
     1501 gacattgatt tcattttgtt tgctttgtag ttgtgcatcc ttgctgacta attgactttc
     1561 taagttttgt ttttctgctg ccgattgatt tttttggttt tctaattcat ttacttttgt
     1621 ttggtattct tggacatctg cctttgaatc ttgcaatgtc tgttcttttt gtgctagtcg
     1681 agcggtaaga tctgtaatcg cttgctcttt atctgtcaat gtatgatcaa gctgtgtcag
     1741 attctcattg attgtatcaa tatctgtctc tccaccccac aaaataattt catttgcgta
     1801 ccgcgtgcct gtgtagccaa ttagacatag aaatgcaacg acaatcccac cagtaataaa
     1861 tttctttttc atttgcatag ctccttaatc caatactttt acatgctaaa agtaagtgat
     1921 ctcaaacaca atgtcaagcg tttttaaatt acatatgtaa ttttaatata aatactatca
     1981 aatactcatt taatcctcct tttttatcta tttaaaaaat cttttttata gagaaaaatg
     2041 caggttaata ctttcttaaa taaatattta tggtgaacaa aaacaaaaaa gcaagtttta
     2101 ttaaacgtaa cccttttcat ttcatagatt actgtattta ttacaaaaaa cg
//
LOCUS       DACCQI010000202         2144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCQI010000202 DACCQI010000000
VERSION     DACCQI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.212"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(118..708)
                     /locus_tag="IVO76_002772"
     CDS             complement(118..708)
                     /locus_tag="IVO76_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7430695.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="IVO76_002773"
     CDS             1231..1392
                     /locus_tag="IVO76_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430696.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="IVO76_002774"
     CDS             1411..1905
                     /locus_tag="IVO76_002774"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin"
                     /protein_id="HAP7430697.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
BASE COUNT          773 a          332 c          364 g          675 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaagttgag ttttttattt
     2101 taatgaatta agactattaa atagataatt attgtaactt aaaa
//
LOCUS       DACCQI010000203         2081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCQI010000203 DACCQI010000000
VERSION     DACCQI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.213"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1875)
                     /locus_tag="IVO76_002775"
     CDS             complement(<1..1875)
                     /locus_tag="IVO76_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAP7430698.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     A"
BASE COUNT          542 a          451 c          395 g          693 t
ORIGIN      
        1 tgcagcagca atgaccaatt caggttcctc ctcttttgct gtcgaagccc aatcaatgat
       61 tttcaagcct tcattcacta agacggctgc ttcttctgct gagtaaaatt gagcacgcgg
      121 atgtttggaa gagatgatca aattgatttt ttctgacgag cggaatgctt tatccatgac
      181 tgcgagcaat gtatttgcat ccgcaggcag atattcacgg atgaactccg ccttcttttc
      241 tgccaaatgt gtcaatattc ctggatcttg gtggctatat ccattgtgat cttgctggaa
      301 gactgttgag cttgcaatga gattcagcga aggataatct tttcgccaag aaaggtcatg
      361 tgatttccgt atccatttga agtgctgagt caacatagag tcaaccactc gcaagaatga
      421 ttcgtaactt gcaaaaaaac catgccgtcc agtcagaacg tacccttcta agaacccttc
      481 atcttggtgt tccgataatt gactgtcgat gacgcgacca gatggcgaaa gccattcatc
      541 attttctggt tcgatttttt ccatccattg gcgatttgtc gttttgaaaa cttgattcaa
      601 tcggtttgat tttgtttcat ctggtccaaa tatacggaaa ttttctggat ttttctcaat
      661 gatctcgcga acgaatttac caagttcggt catatcttct gctttaacgg agcctggttt
      721 ttcaaattgc aaagtatatt ttttccaatc tggcataata agcggtcttg gatcgattcc
      781 accattggtg ataggatttt ttgccattct ttggtcacca gtaggcgcaa tttctctgat
      841 ttcttctaaa atacgtcctt gtgcatcaaa taatttttct ggttcatagg atttcagcca
      901 acggagtaaa gcatctgcat gatccatatg ttcttgatct acaggaatcg gtacttggtg
      961 tgctcggaag gttccttcaa ttggttcccc gtcccattct tttggaccgg tccatccttt
     1021 tggtgtgcgt acgatcaata ctggccattt tggcatcacg acatctgttg ctgtcttcag
     1081 gcgagcttct ttttggatgg atttgatttt ctcgattgct tgatccattt tttcagccat
     1141 aacaggattt aatttttctg gatcatttcc ttcaataaag aaaggctccc agccaagtcc
     1201 gttaaagtaa tttgctaatt ctccatctga tttacgtgcc aagatcgttg ggttggctat
     1261 ttttgcacca tttaaatgaa ggatcggtaa aacagctcca tctatcactg gattgataaa
     1321 gacatttgag aaccagcttc cagctaaagg accggtttct gcttcaccat caccaatcac
     1381 gacagcagag attacttcag gattgtctaa tacagctccg actgcatgtg aaagagagta
     1441 tccaagctct ccgccttcat ggatagaacc tggtgtttct ggtgctgcat gagaagcaat
     1501 gcctccagga aatgaaaatc gcttgaataa tttttgcatt cctgtctcat cttccgttac
     1561 ttctggatag atctctgtgt aacttccatc taaataagca ttcgatacca tgacttgtcc
     1621 gccgtgtcct ggcccttcga tataaaacat gttcaaatca tatttattga tgactcggtt
     1681 caaatggaca taaataaaat tttggccagg aatcgttccc caatgaccga ttggatgttt
     1741 tttgacatct tctggtttca gtgtacgtct cagtaagggg ttatctttaa gataaagttg
     1801 cccaacagaa agataattag cagcacgcca ccaagccgtc attttatcaa agtattcttt
     1861 actagaataa tccatacata acactccttt tcattattac tcttattcta gcgcagatac
     1921 tcccaattga aacctaataa gcacttctgt tcatttgttt ggattttata tctagccaaa
     1981 tgaacaggta taaaaaacca cgaaattttt cctttcgctg acgtttttta cattgatcca
     2041 aaaaacttgt cgttatcaca atggctgttc cgcagttcat t
//
LOCUS       DACCQI010000204         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCQI010000204 DACCQI010000000
VERSION     DACCQI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.214"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IVO76_002776"
     CDS             complement(63..1403)
                     /gene="glnA"
                     /locus_tag="IVO76_002776"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7430699.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1431..1823)
                     /locus_tag="IVO76_002777"
     CDS             complement(1431..1823)
                     /locus_tag="IVO76_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7430700.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          578 a          452 c          342 g          662 t
ORIGIN      
        1 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
       61 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      121 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      181 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      241 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
      301 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
      361 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
      421 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
      481 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
      541 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
      601 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
      661 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
      721 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
      781 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
      841 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
      901 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
      961 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1021 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1081 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1141 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1201 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1261 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     1321 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     1381 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     1441 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     1501 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     1561 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     1621 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     1681 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     1741 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     1801 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     1861 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     1921 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgcac
     1981 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACCQI010000205         2029 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCQI010000205 DACCQI010000000
VERSION     DACCQI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.215"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(193..378)
                     /locus_tag="IVO76_002778"
     CDS             complement(193..378)
                     /locus_tag="IVO76_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430701.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(1228..1569)
                     /locus_tag="IVO76_002779"
     CDS             complement(1228..1569)
                     /locus_tag="IVO76_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7430702.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1569..1799)
                     /locus_tag="IVO76_002780"
     CDS             complement(1569..1799)
                     /locus_tag="IVO76_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7430703.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          685 a          343 c          275 g          726 t
ORIGIN      
        1 ataacagctg aaaagatagg acttactctt agtaaagtat ctgaactatt aatttttaaa
       61 ctatcatttt tatcctcaac tacatacata tctgttgcaa cttcactttc tgtctcatcc
      121 agataaatga ttaaggaatc gggaattgca atatcttcag ctgacgctgg aatagttgag
      181 ccaaattagt catcatatct ttattataaa ataaccaaca gcaaaaaata caatgaaaaa
      241 caaacttgga actaatgtat cattaataaa tgttgaaaaa ttcagcaact cattactgta
      301 tgattttagc agcccaacca acatataccc tataaaagct aataagatgg ttaggaatcc
      361 tttagaattt ttactcattc ataaacactt ccttaaatag cttttattat agaaggtatt
      421 gtaaataata atattgacaa aaagtgactc aaaataaaaa aaaccttaca ttatactgga
      481 atgaactgta ttgatagtgt acctaaaagc tctactgaca taagatcgtg taatcggtaa
      541 agtaatttta ttaattatac gggatgtaat caattagaga ataaactaat cataaaaggt
      601 acttttacat tcataattgt cgctttgaga ttctactttg gttcactatc tgacccaatt
      661 gcacattttc gagcattaca agtattggtg tcgttagaac aactaaatag aaactagcgg
      721 tagatttttc agaatctatg ctaatttctg tacggatgtt ccagtgatgg caaatttctc
      781 ttttcattgt ttttcttgtc gcatgatatg cctttttgtg ttattcagtt aaactataga
      841 aattctttcc tagacagatc ttgatcccaa attttacgtt tttgagcatc gatatagttt
      901 aaaatgacat tactcatatc taaagagttt agatttttta ggactaaaac tgtcagttgt
      961 tctttttctt ctttctgtta aagtattgtc gcgacttttc gtatacgtta agatatgtcg
     1021 tgaaaaaaca agttttgtga cacaaaaaaa cgaatgactt gaggaaatca cctcaatcat
     1081 tcgtttaaaa tgctatatat tctttagttt aagatacgga aattaatccc atcgaccaca
     1141 ctagtagaat gaacgacatt tacttggcct ttttttaatt tcgttatgtt tagcatataa
     1201 aaatattata gagtttactt acaatgctta aaacatatac tgtataatct gatcaatttt
     1261 agccatgtct tgcaatggta agttttctat ttttttgagt tttcgttcct taaaatcaag
     1321 agattttaat tgtgtaataa ttacttgtcc ttttatatct agctcttcag gtaatgagta
     1381 acgtgtcggt aaattcttta tagtggaagt aattggacat atgaccgaaa acattgtttt
     1441 acggttaaat tcataacgag agacgactag tcctggtcgt cttttttgaa tttcttttcc
     1501 tactgaggga tcaaaatcaa tccatacaat atcacctttt ttaggaatat aaattctttc
     1561 ttctgacact ataaaatttc tctcccttca ggagttaaat cttcccattc gtctttctca
     1621 taatactcgg cctcttcccc accactaaat ggatcttcaa tttttggaac taacgtaatt
     1681 gttccatcat cagaatacac aacgatatat tcttggtttt ctgaaggttt ctttccgtta
     1741 tcagaaggta aagttaccac tacagaactt ccttgtaatc tagattttgt aacaagcatt
     1801 atcatcactc ccttaataaa agtatactat ataacactaa gtaacgcaaa attccccttt
     1861 gtaaactatc tagaaaattg ttttgtttaa agtatatctt attggatata aatagaaata
     1921 aattgtagcc agttcatttt ttctttaaat aaaaaaataa tccaagaaac attgaaccta
     1981 tggatccaaa agctacccaa atactatagc taactccccg ctaccttca
//
LOCUS       DACCQI010000206         2018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCQI010000206 DACCQI010000000
VERSION     DACCQI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.216"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            57..914
                     /locus_tag="IVO76_002781"
     CDS             57..914
                     /locus_tag="IVO76_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7430704.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            915..1463
                     /locus_tag="IVO76_002782"
     CDS             915..1463
                     /locus_tag="IVO76_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430705.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1723..2018
                     /locus_tag="IVO76_002783"
                     /pseudo
     CDS             1723..2018
                     /locus_tag="IVO76_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          775 a          265 c          428 g          550 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaaatc cgcgatgtac attgcggact
      541 gggcaatcgt cgtcttacag acccaagtaa aatcgatgag aaatgcgatg caatatcttg
      601 agtatatgga cttttttgtg aaagaatttg atacaaattt atatgttgct ggtgttattc
      661 ctttcatgtt ggaaagcggt gacgcagtag atcaggaaat gtataaacaa gcccaagaga
      721 tttatgggga acatttgatt aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg
      781 acggatcagg tattacgatg gagaagacaa agaaaggtct cttaaaacaa tgggatcgca
      841 aaagccacga attatttatc agtattttga aagaattgga agaacacgag aattggtatg
      901 cggaggtgga gtaattggct gacatcaaag gacttgatat ttttgggaac aacaaaggac
      961 tcgccaaaat aaaaaaagaa gcagaagcaa agaaaaagca gcaagcaatt acaccatctg
     1021 aaaataagca attagaagtg caccaagttc aagaaataaa aacttcctcc aatgttgaaa
     1081 gacagagtag aggaagaaca aaaaaagttg gcgcaccaat cagaaagttt gataaggttt
     1141 attccaccaa gcatccttta aaactgtcga ctttgttaaa tgcgacttcc agagttttag
     1201 tagaaaagta tgagactaat atgacaagag atgaattgct tagaaaagct ttagatgagt
     1261 atattaaaca gaatcttacg caagaagata agcaagattt gtttaatgac gtggtgaaag
     1321 aactagactt gttcagagaa aaatatccaa ttgttccaga attagatgat cgtggagaaa
     1381 taataagaac taccagtcag ataaaacaag ataccgaaga ctcactgcgc aaagggtggg
     1441 gaatgaataa taaagttaga taataatcaa agaagaacta agtaactacc tttaaacggt
     1501 agttacttag ttcttctttg atttgcatga aatagagggg atttattttt ctggttacgt
     1561 gattaaaaaa tccaaaaaat gagcatagga acggttatga ttttagggtg tgttttaatg
     1621 agatattgtg atttcattaa caagtgtatg ataatctgtt gttgagttag taaataagta
     1681 atagagctaa ctctaaaata aaactattag gaggagatgt aggtgaaaaa gtttaaaaag
     1741 aaaatatatt taggattggt tgttggggtg agtatgtttg ggttcatggc tcatactaaa
     1801 caagtaactg cagatactaa ccaaaaagaa aggattatga gccaattaga tgactctcaa
     1861 ataagtatgt acccaatgag ctaattgtca gtttaaaggc tacacaaaga atttcaacag
     1921 agaaaaataa accgcaaaat ttattgggtg taaaagttat ggtggaaaaa gatttgactc
     1981 ttaggacaaa tgaaagcgat tgtaataagc gagatcaa
//
LOCUS       DACCQI010000207         1981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCQI010000207 DACCQI010000000
VERSION     DACCQI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.218"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            266..1750
                     /gene="guaB"
                     /locus_tag="IVO76_002784"
     CDS             266..1750
                     /gene="guaB"
                     /locus_tag="IVO76_002784"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7430706.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          674 a          357 c          437 g          513 t
ORIGIN      
        1 cctctccact aaacggtgtt caaaagtttg catctgcggt aataaaccca accaattcaa
       61 aaacatgaga agctgttttt gattggctgt tcttattact tgatgcagga cagctttttt
      121 acaatctctt ttaatataca cagatgtaaa cgctttatat tggtattcta tgagaaagct
      181 aacttgattc caagtagatc attgacttaa atgaaaaaat caattatttt aatagataaa
      241 ctaaattaga ggagtggtac taaaaatgtc caactgggaa acaaagttca cgaaaaaagg
      301 atttactttt gatgatgtct tattgatacc agcagaaagt cacgtgttac caaacgaagt
      361 ggatatgagc gtacaattag ctaaaaatat taagttgaat attccaatca tcagtgcaag
      421 tatggatact gttaccgaca gcaagatggc cattgctatg gcaagacaag gcggactagg
      481 tgtgatccat aaaaacatga cgatcagcca gcaagcggat gaagtaagaa aagtcaaacg
      541 ttctgaaagt ggcgtaatca ttgatccgtt cttcttaacg ccacagcatc ttgtagcaga
      601 cgcagaagaa ttgatgagca aatatcgtat cagtggtgtg ccaatcgttg aaacacttga
      661 aaacagaaaa ttggtaggga taattacaaa ccgcgacatg cgttttgtaa cagattatca
      721 catgccgatt gctgatgtga tgacaaaaga caacttagtg actgccccag taggtacctc
      781 tctaaaagat gcagaaaaga ttttgcaaaa acataaaatc gaaaaattac caatcgtaga
      841 taacgaaggt cgtttaagcg gattgatcac gatcaaagac atcgagaaag tgatcgaatt
      901 tccaaatgca gcaaaagatg aacatggtcg attgcttgta gcagcagccg ttggtgtaac
      961 aagcgataca tttgagcgtg cgaatgcttt attagaagca ggggcagatg caatcatcat
     1021 cgatacagct catggacata gtgcaggtgt gatccgaaaa attcaagaaa tccgttcaac
     1081 ttttgcagat gcaacattga ttgcaggaaa cgttgcgacg gctgaagcga caaaagcttt
     1141 atatgatgca ggtgtagatg tcgtaaaagt tggtatcgga ccaggatcga tttgtacgac
     1201 acgtgtggta gccggtgtag gtgtacctca gctcacagct atttatgatg cagcatcagt
     1261 tgctcgtcaa tatggcaaag cgattattgc agacggaggg atcaaatatt ctggtgacat
     1321 cgtcaaagca ttagctgccg gcggacatgc agtgatgcta ggaagtatgt tggcagggac
     1381 agatgaatca ccaggtgaat tcgaaatctt ccaaggacgt cgattcaaaa cataccgagg
     1441 tatgggctca ttaggcgcga tggaaaaagg ttcaaaagac cgttacttcc aaggtagtgt
     1501 caatgaagca aataaattag taccagaagg aatcgaaggt cgggttgctt ataaaggaag
     1561 tgttgcagat attattttcc aaatgatcgg cggtttgcgt tcaggaatgg gttatgttgg
     1621 ggcagccaat ctacagcaat tacgtgatga agcgcaattt atccaaatga gcggcagtgg
     1681 attaaaagaa tcacaccctc atgatgtaca aatcacaaaa gaagcaccaa actattcagt
     1741 tgaatcttaa aaacattaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
     1801 ctaaacacgt ataaacggcg gaagtagaag caactccttc ggaaataaga agaaattcac
     1861 aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg
     1921 tcccagcctc tttttgaata aacagtgttc ccttgccacc tccgaatcac aagtacaaca
     1981 t
//
LOCUS       DACCQI010000208         1977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCQI010000208 DACCQI010000000
VERSION     DACCQI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.219"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            128..1852
                     /locus_tag="IVO76_002785"
     CDS             128..1852
                     /locus_tag="IVO76_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7430707.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          773 a          241 c          347 g          616 t
ORIGIN      
        1 agtttgtaac aaacatatat tttttatatt acaatatttc aacaaattaa aaaaaaatta
       61 tatcttctta aacaatgata atattaaaat gcaagcgctt gtaaaaaata aggagaagag
      121 gtaaaaaatg aagaaaaaac tattaacttt atttcttgtg atgtctttct caatcgtttt
      181 gagtgtgtac tacaatacta ttgttttttc agaacaaata aactacacag aagaacagta
      241 taatatagaa aaagaaaaag ctttaaaagt tggttatagt gaagaacagt ttaagcagat
      301 tatggaaata cctacaaatt tatcaaatga aaatagtgaa acaagaatag taaattatac
      361 tatgacttct aatcaaacca aagtcattaa taaagcaatg gagcaaattg gaaagccata
      421 cgaatgggga gcatcgggac caaattcttt tgactgtggt ggtttagtga aatatgttta
      481 caaacaagct gtaaatattg aactgcctat gggaaccacc aatcaagagc aatacggtac
      541 agaggtaagt ttaaattcac ttaagcctgg tgatttatta ttttacggca atcgaggtgc
      601 aacttatcat gtaggtatat ataaaggtaa cggtgtaatg attcatgcac cgcaacctgg
      661 agaaactgta aaagaggtta acatacaata tttttatcct aactttgcaa aaagaatttt
      721 gcctgatgaa cctgactacc cttatattga ctataataaa atggtaactg ttacaaaagc
      781 ttggtcaata tggaatgatt tacaatttag tcatgaaatt aagaaagcta ttattggaga
      841 taattacaag atagggaagg tttatactaa tccagaaaat aataataaat atggagaaat
      901 attagtaagt aataaagtat atggatacat taatttttat gctgttaaag agttgacctc
      961 tgttcaaata aacagatatt taacttctaa agatagtggt caaccaactt ggggaaatct
     1021 agagtgtact ataagtaaag gacaaactac aaaagataaa atatattttg ttaaaggagc
     1081 atataattta ggggatggaa agtatctata ttctatttat aaagatcagg attcctctga
     1141 atggttaggg tatttaaaag cacatgttag tttagcctat actcctattg aagaaattaa
     1201 taaaaacgta actgttacta aaaattggtc gatttggaat aatctacaat gggaaaagga
     1261 aattgaaaag cctcagattg gttctgtatt ttcagctaga ttaaagttaa ccaatgtatc
     1321 gaataatgct gtctattaca agctttataa atctggaaaa ttttatggtt atatcaatgc
     1381 tgaggctgtc aaagacctta caacaacaaa gcttaataag tatgttacat ttagtgttaa
     1441 taatgaagat ttttggtctt ctttagatgt aaattattca aaaggaaaaa ctgaaagagg
     1501 ccgagtattt tacattagta tttcatataa taccgcagat aaccaaccga tttattcagt
     1561 atatacagat gaaacatgta cggaatggcg agggtattat aagggaaaca actttgaaga
     1621 cactcaaatt actatgcttg agaataaaag cgtgaaagta acaaaaagtg ggtatacagt
     1681 atggggagac ttgaattttt ggactaagtc aggaatatcg aacacaggag atatatacac
     1741 cactgaccga aaattttata attttaccaa caatgcttac tattatgaat tgaaaaaaga
     1801 tgggagggtc tatggctata taaatagtga agcagctgta gagatgaact aaaatattgc
     1861 tgtcccttaa tcttatgggg cagcaatatt tttatattat taatcatttt tctgaagcga
     1921 gggtagaaga attaatcaat agtttttctt gttttgtaat ataatagttt aattacc
//
LOCUS       DACCQI010000209         1936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.220, whole genome shotgun sequence.
ACCESSION   DACCQI010000209 DACCQI010000000
VERSION     DACCQI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.220"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IVO76_002786"
     CDS             complement(14..706)
                     /locus_tag="IVO76_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7430708.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IVO76_002787"
                     /pseudo
     CDS             791..967
                     /locus_tag="IVO76_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IVO76_002788"
     CDS             complement(1143..1712)
                     /locus_tag="IVO76_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7430709.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..>1936)
                     /locus_tag="IVO76_002789"
     CDS             complement(1786..>1936)
                     /locus_tag="IVO76_002789"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430710.1"
                     /translation="KPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
BASE COUNT          521 a          384 c          344 g          687 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acctatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aacgtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtgtgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggctta
//
LOCUS       DACCQI010000210         1897 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCQI010000210 DACCQI010000000
VERSION     DACCQI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.221"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            12..>1897
                     /locus_tag="IVO76_002790"
     CDS             12..>1897
                     /locus_tag="IVO76_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7430711.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPD"
BASE COUNT          644 a          338 c          421 g          494 t
ORIGIN      
        1 agggaagaag aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgattc
       61 gtgtcctcgg ggaaggacat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
      121 atctttggag caatttacgt caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ctcctttgac cgataaagag tttgaaacga tcaaaaccga attattgcta cggacgaaaa
      241 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
      301 aacgggaaaa tccgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
      361 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcgcggga tcgacgtttt gacgtcacct tattgatcaa tggcttaccg attatccaaa
      481 tcgagctaaa acaagtaact gcaaaagatg gtttttatca ggcgtttaat caaatcaaaa
      541 aatatgctga agaaggcatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaacgact cggtattttg caaatgcctt accgaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccagatgccc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagtg catgcgattc
      841 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
      901 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
      961 atcgaacgat catgttagtg gaccgcaaag atttagataa tcagaccacc acagagttta
     1021 cgaaatttgc ttcggagttt aatactgggg tttcttctgg taatgccaaa gcgaatagct
     1081 tgatcgtggg aacaggaagt gccaaagagt taagtgaaac tctgttggca gatgccaatg
     1141 cgaatgttgt gatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     1201 aacaggaaga gaaaaaagga accaatcgct tccaaaaatt aatggggcaa catatcgtgt
     1261 ttgtagttga tgagtgtcat cgagcgttaa gtgctgaaaa tatggaagaa atcaaaaaaa
     1321 tgtttccgaa atccacttgg tttggcttta caggtacacc gattttccct gagaatcgga
     1381 aacaagccaa agggcaattg gcacgtacga cccatgatca gtatggagag gtcttgcaca
     1441 cctataccat caagaatgca ttagaagatg gctcggtttt aggatttcaa gtagagcatg
     1501 aaaacaccgt ggagccgact tcattagaga ataaaatcta tcggaaatta aaagaagtag
     1561 aaacatatgc agagtattca ccggaacaga tcaatcgtat gatcgatcaa atggagtctg
     1621 tcaaaaaaga aagttatttg gaccctactg tttttgaagc agaggaacat atccaaaaag
     1681 tcattcataa aatgttccgt ccagacaatg cctatacaaa atttgatttt cgaaatggcc
     1741 gtccaacgaa gtctgcgatc ttaaccacga gttccattga tatggccaaa aagtattatc
     1801 gggccatcaa agagatgacg aaagaatctg attggttaat aaaagagttt cccaatcagc
     1861 ctatccgtga aggacgaacg atggaggatc ctgattt
//
LOCUS       DACCQI010000211         1857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCQI010000211 DACCQI010000000
VERSION     DACCQI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.222"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..435)
                     /locus_tag="IVO76_002791"
     CDS             complement(<1..435)
                     /locus_tag="IVO76_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7430712.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFI"
     gene            complement(456..729)
                     /locus_tag="IVO76_002792"
                     /pseudo
     CDS             complement(456..729)
                     /locus_tag="IVO76_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            complement(752..>1857)
                     /gene="lacG"
                     /locus_tag="IVO76_002793"
     CDS             complement(752..>1857)
                     /gene="lacG"
                     /locus_tag="IVO76_002793"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7430713.1"
                     /translation="ECHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFC
                     FEEFPEVNYWTTFNEIGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLY
                     KDKGYKGEIGVVHALPTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTME
                     GVNHILAENGGELDLRDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKG
                     EKGSSKYQIKGVGRRIAPDYVPRTDWDWIIYPEGFYDQIMRVKNDYPNYKKIYITENG
                     LGYKDEFVDNTVYDDGRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEK
                     RYGLFYVDFDTQERYPKKSAHWYKKVAETQVIE"
BASE COUNT          559 a          383 c          308 g          607 t
ORIGIN      
        1 aataaagtaa ggcattgttt tttccatttc caagttcgtt acctgaaatt cggttctaat
       61 tgatttacca ttcaaggtat aaacaactct cagttcaaac tgatataggt aattatcaag
      121 catctcagca tttggtttaa tactaaaggt cacgctatta tcattgactt cttccagaat
      181 aaattcttct tttcgaacaa aaccatgtct acgaataaga cctgtaaaaa aaggtctttc
      241 ttgaggtcta taaatagccc aatcatttcg taaactacca cagataggaa atagaatagg
      301 cgcctgtcca ttccaataat ttggatcagc ttgccagaga tactctatcc catccttgtc
      361 tttaatcgaa gtcagttgac caccaagtgt tttaaactga acagtaagat actcgttttt
      421 taattcgata gtcacaacta attcctttct ttttattatt ggaaaggcaa ataatttacc
      481 tcgaagtgaa tagtctcacg tatttctaca tcgagtgatt gtaaaaattc ccattcaaat
      541 gctgataact ctaaatcaga atggcaaatc cactcttttc cagttaaaac ataccattta
      601 ccacatttta gcaacggggg tagcttcaag ctcaggaatc ataggacaat cttcgaaact
      661 aacaatatat accttttcaa agcctggtct aaataagtgt tgtacttttt ctaaagtatc
      721 ggataacaat tttttacatt ttcaagtaat attactctat cacttgagtt tctgctactt
      781 tcttatacca atgagctgat ttcttaggat agcgttcttg cgtgtcaaag tctacataaa
      841 atagtccata acgtttttcg tatccgtttg accatgagaa gacatccata agtgaccaga
      901 tgaagtaacc tttaacattt gcaccatccg caattgcgtc tgataaaact tccaagtgtt
      961 tcttcacgta atcaatacga ccatcatcat aaacggtatt atccacaaac tcatctttgt
     1021 atccaagacc gttttcagtg atgtaaatct tcttgtagtt tggataatca tttttcactc
     1081 gcatgatttg gtcataaaag ccttcaggat aaataatcca gtcccaatcc gttctaggca
     1141 cataatctgg tgcgattcgg cgaccaacac ctttaatctg gtacttagaa cttccttttt
     1201 cacccttacc attgtgaatg atttcagtct caccatcaaa ggcttgcatc caatcactca
     1261 tgtagtagtt gataccaagg aaatcgttca aatctttagc tgcttcaagt gcttggaagt
     1321 cttcatcacg aagatcaagt tctccaccat tttcagctag gatgtggttg accccttcca
     1381 tcgttttatc tgaatagtgt ccaaggtagg ttgcatccaa gataaatttg ttatggatga
     1441 tatcttctaa ctcagcggca cgaacatctg ctggattttc tggatcataa gggtatttgg
     1501 ttggcaacgc atgtactaca ccaatttcgc ctttataacc tttatcttta tacaatttca
     1561 cagcacgtgc atgagaaacc atcatattgt ggtgtgattg gaagactttg gcaagatcat
     1621 attgaatgcc tggagggaat ttaccaacca agtattgacc atctccaatt gggccaattt
     1681 cattgaaagt tgtccagtag tttacttctg ggaactcttc aaaacagaaa gcggcataat
     1741 ctacaaagtg ttctatattt tcgcggttta agaaatctcc atctgagtga agaacttctg
     1801 gtgtgtcaaa gtggtgcaaa gtcacgaatg gctcaacatg acgtttgtgg cactcgg
//
LOCUS       DACCQI010000212         1708 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCQI010000212 DACCQI010000000
VERSION     DACCQI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.223"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(127..1518)
                     /locus_tag="IVO76_002794"
     CDS             complement(127..1518)
                     /locus_tag="IVO76_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7430714.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT          548 a          377 c          291 g          492 t
ORIGIN      
        1 ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa aaatttgaga
       61 aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat cacaacctct
      121 ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat cgcgatctaa
      181 atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga cgtttactgc
      241 tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga ttgctactgg
      301 caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac cgactccagc
      361 tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt ccaaactaaa
      421 gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat aaacgccagc
      481 acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg ttgcatcatc
      541 tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag cacttgaacg
      601 tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga ttggattgac
      661 ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg ctacgtaaga
      721 agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg ccaaaacgtt
      781 tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca cgatccgcat
      841 aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt ttggcgcttt
      901 tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga ctccaatcgt
      961 acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa aggataaaat
     1021 ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt cagagattcg
     1081 tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca gcattgttga
     1141 accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca atacagtcgc
     1201 actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa tgatcgaaat
     1261 aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac ctacaatccc
     1321 gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca attgaagcag
     1381 accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa attttgtatg
     1441 gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa ccactaaaac
     1501 tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc catcgaagga
     1561 agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaatttt tttcagtgac
     1621 ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa ttagtaataa
     1681 aaaaacatac ctgtgatagc tttaatct
//
LOCUS       DACCQI010000213         1685 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCQI010000213 DACCQI010000000
VERSION     DACCQI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.224"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(141..1433)
                     /locus_tag="IVO76_002795"
     CDS             complement(141..1433)
                     /locus_tag="IVO76_002795"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7430715.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          483 a          351 c          295 g          556 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa tcctgaacca ttgtatcaat aatagataca tatttcaaat
     1501 aaaatcgaat gatttttatt tttattttat aaaaacgctt aaaaaacgaa ctttatcttt
     1561 ttagaatggt tattaaaaaa gaggttgtaa cagaagtggt cagcttcaag aaataagaag
     1621 gaatgtacga aaattgcttc tcaaattttt gtgaaatttc gacttatttt tcgaagaagc
     1681 tactt
//
LOCUS       DACCQI010000214         1675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCQI010000214 DACCQI010000000
VERSION     DACCQI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.225"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            102..1397
                     /locus_tag="IVO76_002796"
     CDS             102..1397
                     /locus_tag="IVO76_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7430716.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
     gene            complement(1577..1661)
                     /locus_tag="IVO76_002797"
     tRNA            complement(1577..1661)
                     /locus_tag="IVO76_002797"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1625..1627),aa:Leu,seq:caa)
BASE COUNT          535 a          334 c          313 g          493 t
ORIGIN      
        1 ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc
       61 tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat actcagctta
      121 tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta
      181 aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag
      241 aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa
      301 ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac
      361 aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa
      421 aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata
      481 cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca
      541 tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct
      601 ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg
      661 gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct
      721 atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt
      781 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
      841 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      901 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      961 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
     1021 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
     1081 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
     1141 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
     1201 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
     1261 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg
     1321 cgtacggctt tcgcaacttt tacaatttca aactacgaat tatgattcgt catggcaaaa
     1381 catttttaac caaataaaca gaacagaaag aggttttctt ctttctgttc tgtccaaatt
     1441 attctaagat ttctctttac caacatcatt tgacaaagag ccgataatac ttgtatcgaa
     1501 ttgatttgga ggtaattaca taaaaacaaa aaaaatatag caactctcta taagaaagtt
     1561 gctatattga taattatacc ggcggccggg gtcgaaccgg cacgtcctca acggacactg
     1621 gattttgagt ccagcgcgtc tgccaattcc gccacgccgg caaatttaaa aataa
//
LOCUS       DACCQI010000215         1672 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCQI010000215 DACCQI010000000
VERSION     DACCQI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.226"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(156..1297)
                     /locus_tag="IVO76_002798"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="IVO76_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7430717.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          444 a          323 c          354 g          551 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc ttttcttagt
     1321 agttagattc taccaaatcg tgtccatgaa atcatcctaa catcacattg aagagtccat
     1381 ttcactcaca gagtgttagt tgttaagtga ataaaagaag taaaagagtc ctgaacgtga
     1441 ttgtccagga ctcttttttt caatgaattg ttttccgcca actcattgag tggtgcgatt
     1501 tgcgactaca ttatatcaaa agaaagagaa gggaggaagc agaaatggtt cattcaacga
     1561 ttggagttgt tgttgaaaaa agcagagaca atctcgtttt tgtgacagaa attcaaacag
     1621 gcagatcttt tgtcgtcaca gacaaagcgg caaaagctta tcaaagtagc ga
//
LOCUS       DACCQI010000216         1665 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCQI010000216 DACCQI010000000
VERSION     DACCQI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.227"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            132..506
                     /locus_tag="IVO76_002799"
     CDS             132..506
                     /locus_tag="IVO76_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7430718.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="IVO76_002800"
     CDS             complement(620..1249)
                     /locus_tag="IVO76_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7430719.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="IVO76_002801"
     tRNA            complement(1383..1455)
                     /locus_tag="IVO76_002801"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          558 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tactt
//
LOCUS       DACCQI010000217         1638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCQI010000217 DACCQI010000000
VERSION     DACCQI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.228"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            107..1009
                     /locus_tag="IVO76_002802"
     CDS             107..1009
                     /locus_tag="IVO76_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7430720.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            complement(1049..>1638)
                     /locus_tag="IVO76_002803"
     CDS             complement(1049..>1638)
                     /locus_tag="IVO76_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit EIIBCA"
                     /protein_id="HAP7430721.1"
                     /translation="TETDASGMFQAFIGIAIASVIGFSLTFFFWKDEAGETDDIQKKS
                     GKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKH
                     AIGMVSETGLELLIHVGLDTVQLEGKYFESLVQQGERVKQGDLLLRFDKKKIEKEGYS
                     LETPVIVTNYTDYLDILENQGETVGPDDALITALT"
BASE COUNT          489 a          348 c          290 g          511 t
ORIGIN      
        1 atcttgtcaa aaaaagtcta ttttaagtta tatagtataa atccgtttac aaaacatttt
       61 caaaaaagtt ataatttacg taaaaaaata agaagaaggt aactacatgt tattacgtga
      121 aaaaatcaaa gaaatctcct tttctccttc agaaaatgaa gtgatccagt ttctcttaag
      181 cgattctcca gcacttggcg aaatgaccat ccaagagatc gctgagcgat gctatgtcca
      241 cccgtctaca ctcatccgtg tagcaaaaaa aatgggtttt gatggctggg tcagttttcg
      301 taatgctttt ttagctgaaa cagcctatct tgaagggaat tttcaagacg tagatgcgaa
      361 tcttcctttt tccaccacag acggtattat gacgatcgca aataaaatgg cttctttaga
      421 acagatgacg attcatgata cactttctct cattcatcat gaccaactgc aaaaagccaa
      481 acaactaatg cttcaagcaa aaaaaatcgt gattttcgcc agtaactcga atactttgat
      541 ttctcaagat ttcatgctga aaatgaagcg catcaaaatg gatgtcacga ttgtcaccac
      601 ttttggtgaa tcgttctatg aagcgtacaa ttgcacgagt gatatgtgcg ggattctgat
      661 ttcttatacg ggtgaaaaca atgtgatcct tcgtaccgca gaaatattga aacaaaatga
      721 tgttccaatt ttagctttga ccagtatcgg tgattcaacg ctatctgcaa tgagtacagc
      781 cgctttgcga cttacgacac gcgagcgact gtattcaaaa attggtaatt ttacgatcaa
      841 tacatcaatt tgttatcttc tagacgtgtt atatagttgt gtttttgcag aaaattatca
      901 acagaattta gtccatctaa tttctgtcgg tcaaaaattc gataaacgcc ctatcagctc
      961 agccatcatg aaagagcctt tcaattttga aaataaagaa gtggaataaa aagaatagtt
     1021 aagcctagtc cattcttcac ctaaccgttc aggtcagtgc ggtgataagt gcatcgtctg
     1081 gcccgaccgt ctccccttgg ttttccaaaa tatccaaata atccgtgtaa ttggtgacga
     1141 tcacaggtgt ttctaaactg tatccttcct tttcgatttt ctttttgtcg aagcgtagca
     1201 acagatcgcc ttgtttcact ctttctcctt gctgcactaa gctttcaaaa tatttccctt
     1261 ccaactgcac agtatccaat ccgacatgaa tcagcaattc cagccctgtt tcagaaacca
     1321 ttccaattgc atgtttcgtc gggaacaggg tcatgactgt tccgtcaaaa ggggcacgta
     1381 cttctccttt ttcagggtag atcaagattc ctcgacctag tgttccttgt gcaaaagctg
     1441 catcctttgc tgcatttagt ggagcgattt gtccttgtat cggtgttttt acaatttctt
     1501 tacctgattt tttctgtata tcatctgttt cacctgcttc atccttccaa aagaaaaaag
     1561 tcagcgaaaa cccgattacc gaagcaatag caatgccgat aaacgcttga aacataccac
     1621 tggcgtctgt ttccgtga
//
LOCUS       DACCQI010000218         1626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCQI010000218 DACCQI010000000
VERSION     DACCQI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.229"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..450
                     /locus_tag="IVO76_002804"
     CDS             <1..450
                     /locus_tag="IVO76_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430722.1"
                     /translation="AELLRLETEKYIMNVYLERSELVENFVSYTICRKHYYSESEYYS
                     IFESMELMEELNSKDATVDDYYLVFTKFLTCGTESIVLMGMDYELAVPEVFLREYVEE
                     VDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRKRMPYQRFERKKGQ"
     gene            831..1010
                     /locus_tag="IVO76_002805"
                     /pseudo
     CDS             831..1010
                     /locus_tag="IVO76_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1069..1422
                     /locus_tag="IVO76_002806"
                     /pseudo
     CDS             1069..1422
                     /locus_tag="IVO76_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          593 a          239 c          318 g          476 t
ORIGIN      
        1 gcggagcttc tacgactaga aacagaaaaa tatatcatga atgtttacct agaaaggagt
       61 gaactggtcg aaaactttgt cagctacacg atttgcagga aacactatta ctcagaatca
      121 gaatattatt caatttttga gagcatggaa ttaatggaag agctaaacag caaagatgcg
      181 acggtagacg attattacct tgtatttact aaatttttga cgtgtgggac ggaatctatc
      241 gtgttaatgg gaatggatta tgaactcgct gtacccgaag tatttttgag ggaatatgta
      301 gaagaagtag ataacccagg cgacgactgg tacacgactt taactgattt tttagatacc
      361 tacacttacg acgatacaga agaaatccaa aatgaacttg accgtaaacg aatgccatat
      421 caacgatttg aaaggaaaaa aggtcaataa ccatttatac cgaaaagtga gaatgtgtga
      481 aataagaaca aaaaaccttg aaattaaggg aatggttctt atttcgcacc aatcgctcca
      541 accgcccccg ccgcttaggt ggtgcgaaat aagcgaacaa agcccgaaaa cctaagagaa
      601 agaaggagat ttatatgaac aaaattattc ccttattatt agtcctattt gttggagaaa
      661 tacttatgcg acttagtgca gctgtacatt taaacctcgc agtatttttg gtaatactaa
      721 tgttttgttt gatcttttgg cgatttgtgt tagcccttgt gagtctaata ttcaagttga
      781 ttgcagctat atttttcata gggttatttc gatgaaagga gagagtacca atgccaattg
      841 caataggaaa taaacggtta cccgtgacgt tggatgaaaa gagacaaaaa gaattgcagt
      901 aaccaaagca gaagtacggc aaaagtgaat ccaggattat gtgtattgcg ttagatctat
      961 tgatcgcgca agaaaaagca ggatttgatg taccagcact aaaaaagtga cgtcactttt
     1021 taacttgatc cgtataagtg atatcacttt tagcaaggag ggaaataaat gttaaaaact
     1081 aaaaagaatt cgtacaaatt aaagcttagt atactatcta ttatttgtct tgtagatatg
     1141 ataattagtg gtcatttgta tagagtggca tatacgcagt ttattgagta caaggttcac
     1201 tatttaggat tagctgggaa tcgatacgtt gaatatgtcc attattcatt aaaatgttct
     1261 tcgtatattc ttctttggct tattttttca ttgtttgaat tgctaaattt aatagcaata
     1321 tcttcatatc acttagataa gctaaacacc aaatctagcc gttataacga agtgttcaag
     1381 gaaaatatga agttagaaag tgacttaaac aagctaaaaa aaataaaaag aagaaaacat
     1441 aagttaacta aaattatttt attaagaatg atggaatata aggataaggc tgagagtgta
     1501 aaatattttg tgtaaataga aaaaaggaag tcccttctgt agaatagagt taccacaaca
     1561 cattcacaga aaagaggact tccatatgaa cgattttact acagaaattc taaagactct
     1621 agcgaa
//
LOCUS       DACCQI010000219         1569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCQI010000219 DACCQI010000000
VERSION     DACCQI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.230"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            142..1005
                     /locus_tag="IVO76_002807"
     CDS             142..1005
                     /locus_tag="IVO76_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7430723.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            1110..>1569
                     /locus_tag="IVO76_002808"
     CDS             1110..>1569
                     /locus_tag="IVO76_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7430724.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKR"
BASE COUNT          563 a          245 c          316 g          445 t
ORIGIN      
        1 aaaggggagt atgacacaac tgttgtcata ctcctttttt cacctttcca aaccaaggct
       61 cagctattca agtagataac attatgttat gatagcaaag aaatgtatta agaaaacctt
      121 tcactataga gggagaaaaa aatgaacgat ttattagcaa ctatatttac aggtattatt
      181 attgatgaaa atgaaaaaaa ctactttgtc caaaaaaacg gacaaacctt tggcttaagc
      241 aaatcagaag gtgaacacaa aatcggtgaa gcagttgaag gattcggtta ccaaaatcaa
      301 aaacatgaaa atcggatcac tacacaaatt cctaaaagcc gtatcggtca ttacgctttt
      361 ggaatcgtta ctgctacacg aaaagattta ggtgcgtttg tcgatatcgg tcttccagat
      421 aaagactttg tcgtatctct tgatgaattg ccgacaatga cagagctatg gcctaaaaaa
      481 ggggatcgct tgatgatttc ccttagagtt gatgcaaaag accgtatttg gggcagttta
      541 gcagatgaaa aaatattcaa atctttaagc aaatatggaa cagaagaaat gaaaaacaaa
      601 gatgtgatag gaacagcata ccgcttgaaa ttaacaggta cgtacgtttt aacggaagat
      661 ttctatcttg ggtttatcca tccttctgaa cgatataatg aacctcgtct aggcgagcag
      721 ataaaaggtc gagtgattgg tgtccgacca gacggtgtac tgaatctttc attgaagcca
      781 agagcatatg aagctatatc agatgatgca caaatgattt taaccatttt agaacgttct
      841 gcagatcagc aaatcaactt tacagataaa tcggatccag aagaaatcat gaaagctttt
      901 ggtatcagca aaggagcatt taagcgcgca ttaggtaatc ttttgaaaca aggattgatt
      961 acacaagaaa acggtgtgac aaaactagtc aaaaaatcta attgagaatc ccttgcatgt
     1021 ttttcttaac tagcttataa taattttaat taacatagtg ttatatcttt ttttatttgt
     1081 aattattata aattgttggg gttgagtaaa tggtcatgaa tgctatttcg gcgatgaaaa
     1141 aaacaaaaca gcagctacat gaagcggggt ttaagctgac accgcaaaga gaagctactc
     1201 tgcttgtgct tttggaaaat gagaaagagc atttatctgc agaagaaatc ttctttttag
     1261 tgaaaaaaaa gagtccggaa attggacttg ctactgttta tcgaaccctt gaaatcttga
     1321 cagaattgaa aattgtcgat aaagtaagtt ttaatgatgg attagcccgt tatgatttga
     1381 gaaaagaagg cgccaaacat ttccatcatc atttactatg cttgaactgc ggcacgatcg
     1441 aggaagtaga agaggatctt ttacttgaag tggagcaagt agtagaagaa cgttatcatt
     1501 ttttagtaaa agatcatcgg ctaacgttcc atggaatctg tcaggaatgc tatgaaaaag
     1561 aaaaaagaa
//
LOCUS       DACCQI010000220         1514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCQI010000220 DACCQI010000000
VERSION     DACCQI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.231"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..238
                     /locus_tag="IVO76_002809"
     CDS             <1..238
                     /locus_tag="IVO76_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAP7430725.1"
                     /translation="QILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEEQVAKLRE
                     LFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            258..1478
                     /locus_tag="IVO76_002810"
     CDS             258..1478
                     /locus_tag="IVO76_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7430726.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
BASE COUNT          525 a          273 c          305 g          411 t
ORIGIN      
        1 ccaaatattg gattcaccag agttaaaggc acgaattatg ccaagctcag aatttttatt
       61 gggtcattca ctccaagcct ttgatgagca aggacgactc attgatgaag aacaagtcgc
      121 aaaattacgt gaactattca aagatttctt actctttgtc acaatcacta gtcaattgca
      181 acatgcgcac caagcaaata aacgtgaagc cgaaagcttt tcatgggaaa cgatttaatc
      241 gaggggagca aacaaagatg aaattaatag gtatcgtcgg atcagcagct gaactttcat
      301 ataaccgtaa attattaaca tttatccaaa aacaatatca tgaactattt aatttagaaa
      361 tcattgaaat caaagattta cctctgttca atcaagatga tgatcaaaca aatagtcctg
      421 ctattcaaca gctaaatcgc aaaatcttaa tggctgatgg ggtaattatt gctacgccag
      481 aacacaatca tacggttcca gcaggattga aaagtgcact tgaatggcta tcatttaaaa
      541 tccatccact tgaaaataaa cctgtaatgg ttgtgggtgc ttcttactac gatcaaggaa
      601 cttcacgcgc gcaattacac ttgcgccaaa ttttggacgc accaggcgtg aatgcattag
      661 tgatgcccgg aaatgaattt ttattgggaa aagcaaaaga agcatttgac caagaagatc
      721 gcttgattga tccacgaact gttgactttt taggcagtac attagcaagt tttgtaaaat
      781 tcattaaagt cgtgtctacc ttagcagggc ctaaaaagct tccaccagaa gatttgcatg
      841 cgacaggaac aattgctacg acaatacccg atgttgatat gtcagatgat cattggttgg
      901 aaaaagcttc tgaagcagtc ggtgccgttt ctggaaatac ttacgtcaaa ttaaatcgtg
      961 ggttattgac agtggatcaa atcaattact ttttagattc gatgccgatg gaattaactt
     1021 ttgcagataa taataaccaa tttttatatt ataatttgcg ccaagaagcg aaagagatgt
     1081 tagctgctcg tgtgcccggt caggtaggga atccattagc caactgtcac ccagaaaaat
     1141 cgtataaaaa tgtagagtgg gtaattcaac aactgcgaag cggagcaaca gatgcggtac
     1201 gtgttcatgt accaaaacaa gtgccagaaa aatatattgt gcataattac caagcaatgc
     1261 atgatgaaaa tggagagtat gctggaatca atgaatacat tcttgatttc aaaccaatca
     1321 ttgagtggta tttacaacaa acgggtcaac aacttgttgg aaatgaagat gcggtttcaa
     1381 gtgcatctgt tcatggtgga aaaacagatg cagtaaccag cgccagcaca cacgatgaaa
     1441 ctgcagtaga tgcagtttcg agtgcttctg taaaataaaa aggttctgtt gcaaagtttt
     1501 aaataaagaa taaa
//
LOCUS       DACCQI010000221         1503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCQI010000221 DACCQI010000000
VERSION     DACCQI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.232"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            8..1201
                     /locus_tag="IVO76_002811"
     CDS             8..1201
                     /locus_tag="IVO76_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7430727.1"
                     /translation="MRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLTSSQQKGIV
                     LQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGIISYFYPVF
                     TLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEEEQQKIGTF
                     FKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGELG
                     KTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPIKD
                     VPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPRYS
                     QVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKSFL
                     QKMFV"
     gene            1214..>1503
                     /locus_tag="IVO76_002812"
     CDS             1214..>1503
                     /locus_tag="IVO76_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7430728.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITI"
BASE COUNT          532 a          235 c          310 g          426 t
ORIGIN      
        1 accagaaatc cgtttcccag gatttacaga tgattgggaa cagaggaagt tgggggactt
       61 cgttgtagat tatgttgaaa agacaagtgt acaaaatcaa tttcctatgc taacttcatc
      121 tcaacaaaaa gggatagttt tgcaagaaga ctattttgca aatcggcaag taactacaga
      181 aaataatatt ggttactttg tactacctag aggatatttc acgttccgta gtcgaagtga
      241 taatgatgtg tttgtgttta accgtaatga cattattgat agagggatta taagctattt
      301 ttatcctgta tttactttaa aatctgctga ttccgacttc ttcttaagac gtattaacaa
      361 tggaatccaa cgacaattat ctattcaagc agagggaaca ggtcaacatg ttctgtctct
      421 aaaaaagttt aaaaatatag tagcaatgtt tcctagtgag gaagaacaac aaaaaatcgg
      481 caccttcttc aaacaacttg acgacacgat cgctcttcat cagcgtaagt tagatttgct
      541 gaaggaaacg aaaaaaggat tcttacaaaa aatgttccct gaaaacggag caaaagtgcc
      601 agaaatccgg tttcctggtt ttacggaaga ttgggaagag cgtaagttgg gagaattagg
      661 taaaactcaa tcgggaattg gatttcctga tgccgagcaa ggtggttctg aaggtactcc
      721 attttttaaa gtttccgaca tgaataacat tggtaatgag tatgaaatga gaaatgcaaa
      781 tcattatgta tctaatgagc aaattgagcg taaaaaatgg aagccaataa aagatgtgcc
      841 agcgattata tttgctaaag ttggcgcagc aataatgctt aatagaaaac gtttggtcac
      901 aagccctttc ttaatagata acaataccat ggcttattta tttaataata cttgggatat
      961 ctattttggt aaaatattgt ttgaaactat aaatttgcca agatactccc aagtaggggc
     1021 attgccttct tataacagtt ctgatattga gaatatcagt gttaaggttc ctgtaaagga
     1081 tgaacaacaa aaaatcggta ctttcttcaa acagctagat gatacgatcg ctcttcatca
     1141 acgtaagtta gatttactaa aagaaacgaa aaaaagcttc ttacaaaaga tgtttgtcta
     1201 ggagggaaga agaatgaaaa ttagtcaccg tgaggaagca gaagtcgaag aacagttgat
     1261 tcgtgtactt ggagaagggc ataatcagtg gacgtatcgt ccggatctaa aatcagaaga
     1321 agacctttgg gtcaatttac gccaaaaaat tatctcaaat aatcaagcag aactgaatga
     1381 ttttcctttg accgataaag aatttgaaac gatcaaaacg gaattactgc tgcgaacgaa
     1441 aaccccattt gatgctgcga aatggttaaa aggtgaaaat gggatggctc ggatcacgat
     1501 tga
//
LOCUS       DACCQI010000222         1472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCQI010000222 DACCQI010000000
VERSION     DACCQI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.233"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>1472
                     /locus_tag="IVO76_002813"
     CDS             <1..>1472
                     /locus_tag="IVO76_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7430729.1"
                     /translation="RDRRFDVTLLINGLPIVQIELKQVTAKDGVYQAFNQIKKYAEEG
                     MFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKFLFSWRTKDNEKVENLYEFCKQV
                     LNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAIQALFIAANKHQSGYVWHATGSG
                     KTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTTEFTKFASEFNTGISSGNAKANS
                     LIVGTGSAKELSETLLADANANVVIITTRQKLDAALKYAKKQEEKKGTNRFQKLMGQH
                     IVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQYG
                     EVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQINRM
                     IDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTTSS
                     IDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPD"
BASE COUNT          512 a          267 c          307 g          386 t
ORIGIN      
        1 cgggatcgac gttttgacgt caccttatta atcaatggat taccgattgt ccaaatcgag
       61 ctgaaacaag taactgcaaa agatggtgtt tatcaggcat ttaaccaaat caaaaaatat
      121 gcagaagaag gtatgtttcg gaacaatatc ttttcgacct tacagttgtt tgtggtttcc
      181 aatgaacaaa ccactcggta ttttgcaaat gccttaccga aagacttgca ccctaagttt
      241 ttatttagtt ggcgaacgaa agacaatgaa aaagtagaaa acttatatga gttttgtaaa
      301 caagtgttaa atatcccgga tgctcatcgt ttgattgcgg attatacaat cgtcagtgaa
      361 gatcaagaca ataaaaccct catggtgtta catccttatc aagtacatgc gattcaagcc
      421 ttgtttattg ccgcaaataa acatcaatca gggtatgtgt ggcatgcgac tggttcagga
      481 aagacgttga ctagttttgt atctactaag cttttagcac gaaaatcggg gatcgatcga
      541 acgatcatgt tagtggaccg caaagattta gataatcaaa ccaccacaga gtttacaaaa
      601 tttgcttcgg aatttaatac tgggatttct tccgggaacg ccaaagcgaa tagcttgatc
      661 gttggaacgg gaagtgccaa agaattaagt gaaaccctgt tggcagatgc caatgcaaat
      721 gttgtaatca ttacaacccg tcaaaaacta gatgcggcgt tgaaatatgc aaagaaacaa
      781 gaagagaaaa aaggaaccaa tcgcttccaa aaattaatgg gccaacatat cgtgtttgta
      841 gtagatgagt gtcatcgagc gttaagtgct gaaaatatgg aagaaatcaa aaaaatgttt
      901 ccgaaatcca cttggtttgg ctttacaggt acaccgattt ttcctgagaa tcggaaacaa
      961 gccaaagggc aattggcacg tacaacccat gatcagtatg gagaggtctt gcatacctat
     1021 accattaaga atgcgctaga agatggctct gtcttaggat ttcaagtaga gcatgaaaac
     1081 accgtggagc cgacttcact agagaataaa atctatcgga aattaaaaga agtagaaaca
     1141 tatgcagagt attcatcaga acagatcaat cgtatgattg atcaaatgga acctgtcaaa
     1201 aaagaaagtt atttggaccc agctgttttt gaagcagatg aacatatcca aaaagtcatt
     1261 cataaaatgt tccgtccaga taatgcctat acaaaatttg attttcgaaa tggcaggcca
     1321 acgaaatctg caatcttaac cacgagttcc attgacatgg ccaaaaagta ttatcgggcc
     1381 atcaaagaga tgacgaaaga gcctgattgg ttaacaaaag agtttcccaa tcagctcatt
     1441 cgtgaaggac gaacgatgga ggatcctgat tt
//
LOCUS       DACCQI010000223         1452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCQI010000223 DACCQI010000000
VERSION     DACCQI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.234"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            162..1163
                     /gene="ccpA"
                     /locus_tag="IVO76_002814"
     CDS             162..1163
                     /gene="ccpA"
                     /locus_tag="IVO76_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7430730.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT          520 a          239 c          297 g          396 t
ORIGIN      
        1 tgatttatta aaagaccaac gttctaaaaa ctgaaaaaag taaaaatttt catgaaaaca
       61 gagataaaac ggttgcaatt cttgagatta tttgatatcc tattttagaa atgtaagacg
      121 tattttcaga aaacatataa aatatgaata ggagcacaca aatggaaaag caaacaatca
      181 ctatctatga tgtagcgaga gaggcaaatg tttcgatggc tactgtttca cgtgtcgtaa
      241 atggcaatcc aaacgttaaa ccagcaacaa gaaaaaaagt cttggaagtt atcgatcgct
      301 tggattatcg tccaaacgca gttgcgcgtg ggttggcaag taagaaaaca acaacagttg
      361 gggtaatcat ccctgatgtc agcaatatgt tctttgcttc tttagcacgt ggtatcgatg
      421 atgtggcaac aatgtacaaa tataatatta tcttagcaaa ttcagatggc aacgaccaaa
      481 aagaagtcaa tgtattgaat aacttactgg ctaagcaagt ggatggcgtg atcttcatgg
      541 gtcaccatat cacagacgaa atccgtggag aattttcacg ttcaaaaact cctgttgtat
      601 tagctggctc tattgatcca gatgaacaag taggtagcgt aaacattgat tatgtatccg
      661 caacaaaaga tgcagtaaac aaattggcaa aaagcggcaa taaaaaaatc gcttttgtca
      721 gtggtgcgtt gatcgatccc atcaatggac aaaatcgtct gaaaggctat aaagaagcat
      781 taagcgaaaa taacctacca tatagcgaag gattgatctt tgaagcacaa tacaacttca
      841 aagatggtct atctctagtc gatcggatcc ataatagcgg tgcaacagct gtctatgttt
      901 cagatgatga attagccatc ggtattttgg atggtttatt agaccgtggc gtgaaagtac
      961 cggaagaatt cgaaatcatc acaagtaaca attcactact gacagaagta gctcgtcctc
     1021 gtttaacaag tatcacacaa ccattatacg atttaggggc ggtatctatg cgcctattga
     1081 cgaaaatgat gaataaagaa gaagtcgaag aaaaaacgat tatcttgcct tatggaatcg
     1141 aagaaaaagg atctacaaaa taataaaagg acaaccattc agaggttgtg tgcctgccgt
     1201 ttaactctgc gttacaagga gcaatttcaa aaaacagcat ttcatgtttg atgaatattg
     1261 tgactcgtga ttcggagttg gcaggagaac accgtttatt cgggaaaagg ggttatgaca
     1321 aaattttgtt ataatccttt tcttgtgtct taaaaaagag agaaagtggt tgaaattaca
     1381 tttatcgtat agaaaacaat ccgtgtatat atgctgtatt ttgttcacca ccatcaaaaa
     1441 aataagggaa tg
//
LOCUS       DACCQI010000224         1448 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCQI010000224 DACCQI010000000
VERSION     DACCQI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.235"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            72..482
                     /locus_tag="IVO76_002815"
     CDS             72..482
                     /locus_tag="IVO76_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7430731.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            496..852
                     /locus_tag="IVO76_002816"
     CDS             496..852
                     /locus_tag="IVO76_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7430732.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          533 a          208 c          269 g          438 t
ORIGIN      
        1 tattagctaa taattggtat tttaaaagaa attactgtag aattggatta taattaaaaa
       61 ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa
      121 gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt
      181 taacagaaag tgagatcaaa aagatcttag cagcgactga aggaggagtt caagatatta
      241 tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa
      301 agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg
      361 atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg
      421 aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat
      481 agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg
      541 aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa
      601 cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa
      661 cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta
      721 ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg
      781 attattatcg atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc
      841 ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac
      901 agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta
      961 tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa
     1021 aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt aagtagcaaa
     1081 gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta gaaatgacat
     1141 gataagataa ctgagttgtt aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa
     1201 acttcaaaaa aagttcttga caaataacaa atgataagtt atgataaatg agttgctgta
     1261 agatttcgaa aaacgaaatc aaaaaaactt ttaaaaaaga gttgacaatt acttgtctct
     1321 ctgatatgat ataaaagttg ctacggcagc caaaaacgaa tcggggaaca ctcctgatga
     1381 agtagacctt tgaaaactga acaaagtaag acaaaccaaa tttgtagggc gtcttgattc
     1441 aattcaag
//
LOCUS       DACCQI010000225         1445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCQI010000225 DACCQI010000000
VERSION     DACCQI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.236"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..226
                     /locus_tag="IVO76_002817"
     CDS             <1..226
                     /locus_tag="IVO76_002817"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAP7430733.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IVO76_002818"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IVO76_002818"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IVO76_002819"
     CDS             533..1336
                     /locus_tag="IVO76_002819"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7430734.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          462 a          259 c          344 g          380 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggaca
//
LOCUS       DACCQI010000226         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCQI010000226 DACCQI010000000
VERSION     DACCQI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.237"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1315
                     /locus_tag="IVO76_002820"
     CDS             <1..1315
                     /locus_tag="IVO76_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP7430735.1"
                     /translation="LESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDY
                     HYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRA
                     IRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKV
                     DSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNI
                     EYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDIS
                     ECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFS
                     CNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDI
                     EKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          599 a          103 c          234 g          457 t
ORIGIN      
        1 attagaatca ttaaaaaaac attatacaga gccttgggaa gatgaagttt ttagagtaat
       61 tattgaatat aacaatgttc ctattggata tggacaaata tataaaatgt atgatgagtt
      121 atatactgat tatcattatc caaaaactga tgagatagtc tatggtatgg atcaatttat
      181 aggagagcca aattattgga gtaaaggaat tggtacaaga tatattaaat tgatttttga
      241 atttttgaaa aaagaaagaa atgctaatgc agttatttta gaccctcata aaaataatcc
      301 aagagcaata agggcatacc aaaaatctgg ttttagaatt attgaagatt tgccagaaca
      361 tgaattacac gagggcaaaa aagaagattg ttatttaatg gaatatagat atgatgataa
      421 tgccacaaat gttaaggcaa tgaaatattt aattgagcat tactttgata atttcaaagt
      481 agatagtatt gaaataatcg gtagtggtta tgatagtgtg gcatatttag ttaataatga
      541 atacattttt aaaacaaaat ttagtactaa taagaaaaaa ggttatgcaa aagaaaaagc
      601 aatatataat tttttaaata caaatttaga aactaatgta aaaattccta atattgaata
      661 ttcgtatatt agtgatgaat tatctatact aggttataaa gaaattaaag gaactttttt
      721 aacaccagaa atttattcta ctatgtcaga agaagaacaa aatttgttaa aacgagatat
      781 tgccagtttt ttaagacaaa tgcacggttt agattataca gatattagtg aatgtactat
      841 tgataataaa caaaatgtat tagaagagta tatattgttg cgtgaaacta tttataatga
      901 tttaactgat atagaaaaag attatataga aagttttatg gaaagactaa atgcaacaac
      961 agtttttgag ggtaaaaagt gtttatgcca taatgatttt agttgtaatc atctattgtt
     1021 agatggcaat aatagattaa ctggaataat tgattttgga gattctggaa ttatagatga
     1081 atattgtgat tttatatact tacttgaaga tagtgaagaa gaaataggaa caaattttgg
     1141 agaagatata ttaagaatgt atggaaatat agatattgag aaagcaaaag aatatcaaga
     1201 tatagttgaa gaatattatc ctattgaaac tattgtttat ggaattaaaa atattaaaca
     1261 ggaatttatc gaaaatggta gaaaagaaat ttataaaagg acttataaag attgattata
     1321 taatatatga aaagctatta taaaagacat tagtattaaa tagtttaaaa aaatgaaaaa
     1381 taataaagga agt
//
LOCUS       DACCQI010000227         1387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCQI010000227 DACCQI010000000
VERSION     DACCQI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.238"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(30..1049)
                     /locus_tag="IVO76_002821"
     CDS             complement(30..1049)
                     /locus_tag="IVO76_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7430736.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1060..1266)
                     /locus_tag="IVO76_002822"
     CDS             complement(1060..1266)
                     /locus_tag="IVO76_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7430737.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          381 a          298 c          232 g          476 t
ORIGIN      
        1 atgtaatgct cctcaacgag gagctatttt tatcgtttag gaatatttaa ataccaacgt
       61 ttgtcatgga aatcttgcgc accgccttta gtgtttccct ctggatcatt cgttgcccgc
      121 atcatgacat agactttctt attagggaag ttacgcatgt taaaagatac atgatagcca
      181 acatttccag aagtattata agcttgattt acatctgatc tataaattcc atcagctctt
      241 actcgagcta attctttccc agtattgtaa tccataatga agatatactc gtatttatag
      301 ttagcaatgt gccatccagc tacatgcaaa tttgcatttt cgatttctcc aaactgatca
      361 atgtgggcgt aattcgttcc atctgtcagt gtaggattag ctgcaccagc tcgtgttgga
      421 tcaatgacag gcttgttttc agaagttgtt ggattttcat cggtaaatcc atgagctaaa
      481 tcatatgcta atttttcttt acttacgccc atttcagaaa gataaccgta aggatctgta
      541 tgatcgcccc agatgttttg tgttacccat aaatgcgatt tgattcccgg ttggttataa
      601 ggagtgtcca acgttaatgg aataccatat ttcattgctg aatctctagc caattcaacg
      661 tatgccttgt agtttttctc aaacgttgct ttatcatgtg tgtgttgtaa ctcaatctgc
      721 acaggactgt tggcattagc atacgaacca gcaccgtact gtacataacc aggttgaccg
      781 acttgataaa caattccgcc gtctcccaca atataagcag tataagcgct agtccatgaa
      841 cgttgcatat actgcgcttc attgcgtcct gttgctgttt cattagccgt ttcatgcagt
      901 aaaatatact gattatttgc tacttgtgag ctaccttcat ttgcgcccaa attaaattca
      961 ttgttgatag tataggcaaa tccgttaatt ggcaataaaa aaagagccgt taacaggctc
     1021 atcgcagtaa tagtaatttt ctttttcata tctatcctcc tatttatctg atgaaatttt
     1081 cttatacgtt cgatttgata cacctaaaat actccctaga aatgctccaa aacctgtaat
     1141 aatcatcacc gttatatctg tgtaccgcca gttaacacct tttccgacaa gaccgacaaa
     1201 agtcgctaaa gcggggataa ctactatagc gatccatttc aaaatatcaa aggttctatt
     1261 gttcatacta tccacctccc ttcattggtt gggctacttt ctttttcaat cgcttgtttt
     1321 cttctatcaa tatttctctt tctgaaagca aggcatcaat ttgcccgtcc agttgttcaa
     1381 ttttatc
//
LOCUS       DACCQI010000228         1337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCQI010000228 DACCQI010000000
VERSION     DACCQI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.239"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>1337
                     /locus_tag="IVO76_002823"
     rRNA            <1..>1337
                     /locus_tag="IVO76_002823"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACCQI010000229         1336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCQI010000229 DACCQI010000000
VERSION     DACCQI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.240"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(146..1129)
                     /locus_tag="IVO76_002824"
     CDS             complement(146..1129)
                     /locus_tag="IVO76_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7430738.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT          377 a          285 c          254 g          420 t
ORIGIN      
        1 atataaacgg tgttcaatca actgaccctc ggtattagct caaaaaacga caaaatatga
       61 agagctattt ttgtttggct gttcttatta cttgatacag gacagctttt tcacgacctc
      121 taattcacgg tgttcaagca agaaatcatt gttcactgaa aagcgttgga tgttcttttt
      181 caaaggtctc taagatctct cgtacttcat cagtagattt cgtattcatc aactgaactc
      241 gcaagtcatt tgctcctgga aatcctttga tatagatttt aaagaaacga tgtagtcctg
      301 tgatggaacg cgggatgatt tctgaataat gatcttgcag atctagttgc agacgaagta
      361 gtccgatcag ttctttttct gtatgttccc tcgggtcttt ttcaaatgca taaggattct
      421 tgaaaatccc tcggccaatc atgatgccat caacgccata tttctcggcc aattcttctc
      481 ctgtctggcg atcggggata tctccattga tcgtgattag tgtttgtgga gcgatttggt
      541 cacgtaaagc aatgatttta ggaatgactt cccaatgtgc atctacttta ctcatctctt
      601 tacgcgtacg caaatggaca gaaagattgg ctatatcttg atgaagtagg tgactgatcc
      661 attcttccat ctcagacatt tccgtatagc cgatacgcgt cttgacgctg acaggcaaac
      721 cgccagcttt agctgcttca atcaattgtg ccgctacttc tggacgaagg atcaagccac
      781 tgccttttcc acgttctgct acattaggga ctgggcagcc catgttgata tcgattcctt
      841 ggaagcccat ttctgccata gcaatgctca tttctctaaa aaattctggc ttgtctcccc
      901 aaatatgggc aaccatcggc tgttcatctt ctgtaaatgt taatcttcct cgtacactgt
      961 cttttccatc tggatgacag aaactgtctg agtttgtaaa ctcagtaaag aagacatccg
     1021 gtgctgctgc atgctttacg acatggcgaa atacaacatc tgttacatct tccattggtg
     1081 ctaaaacaaa aaaaggcttt ggcagctctt tccaaaaatt agtagtcatt tatttgttac
     1141 tctccattct atcaagcagt gaagtcgcaa tctttctcaa aaacattgtc attatactag
     1201 gaaacaaaaa aagaagcaaa gtgaacagct taaaagatgt ttctgattga gcaaaaaaaa
     1261 gactgtgaca aaaatcttat cacagtccat tttccggaat aaatggtgtt caaaagcttg
     1321 catttgcggt aataag
//
LOCUS       DACCQI010000230         1289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCQI010000230 DACCQI010000000
VERSION     DACCQI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.241"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1161
                     /locus_tag="IVO76_002825"
     CDS             <1..1161
                     /locus_tag="IVO76_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7430739.1"
                     /translation="KLDDSSSYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQPRMNFLPTVLCMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYK
                     LTQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQL
                     RVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVD
                     ELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQS
                     GISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT          396 a          277 c          248 g          368 t
ORIGIN      
        1 aaattggatg actcttcttc ctattgtgaa cactgtcgat cggttcgtat tatccgcaac
       61 ggttcctata cgacacgcac tcagatgttg aaagtcaaag aaaagttaac tattttagaa
      121 ttaaaacgta cccgttttct ctgccatgat tgtggaagga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct catggaactg
      241 tacgaaaatc aatcacgtaa actaatcgct aagaagtact tcgtctcgga tgggacggtg
      301 acacggatct tgcgtgaagc aacgaagcat taccagccac gtatgaactt ccttccgacg
      361 gtgttgtgta tggatgagtt caaatcaatg aattctgttt ctggttccat gagttttatt
      421 tgtgttgatg ggacaaccaa tcagctattt acgatccttg aagatcgcag actttacaaa
      481 ctaactcaat acttcctgcg ctttcctaga aaggcccgtt taaaggtcaa gtacctagtg
      541 atggacatga atgccagcta ttgtcagttg cttaaaacag tctttccaaa tgcggagatc
      601 gtaactgacc gctttcatat tgtccaacac atcaatcgca gcttcaatca attacgtgtc
      661 cagatcatga atcgttttcg aacctcacac tctgaaaatc agaagaagta ccgtcggctc
      721 aagcgatatt ggaaactctt actcaaagat tccacgacat gggagccact caaacggcat
      781 tatcatcgac tgtttaaacg accaattagt caaacagaaa tcgttgatga gctgttaagc
      841 tacaacgaag aattgcgaac tgcgtatcat ttttgtcaat tattacgcta ttatttcgtt
      901 aaacgaggta cggaaggctt tcaagaaacc cttaaaacaa tttcatctac gttacctcag
      961 tcgtttaaaa agaaattcac gatcttccat tgctaccaat cgggtatttc aaatgccttt
     1021 aaggtgtccc actcaaacgg cgtcactgag gggctgaata acaagatcaa gctgattaaa
     1081 cgaattgcgt ttggttatcg caacttttat aactttcggg cacggatcta tttacaacaa
     1141 ggtctgatct ttgaaaacta aaaaaatatg cggcgcacag aacgcgccgc atatcggact
     1201 aagttttatt caaatttgtc ttcaccaaca cgatttgacg aagagccaaa aaaagttacg
     1261 acagttctat gtcgtagctc tttttttcg
//
LOCUS       DACCQI010000231         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCQI010000231 DACCQI010000000
VERSION     DACCQI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.243"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            223..1008
                     /locus_tag="IVO76_002826"
     CDS             223..1008
                     /locus_tag="IVO76_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP7430740.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          476 a          197 c          191 g          382 t
ORIGIN      
        1 atcagtccca ataaataatt tttatattct gacgcatcca ttttactacg gagattatct
       61 gccgcactaa ataatttttg atttaattct gcacccaaaa agatacactc ctatctctta
      121 tttaaagttg ctatataccc tcttatttta gcatactccc ttttagaaaa agcaaaaaaa
      181 aaagaaaaaa tcttattctt gataacaagc tacgtgacgt atatgcctaa caaaaataga
      241 acttttgaat caatcttaaa tgaaggttta accacagaac cggcatccat tatttttgat
      301 gaagttatcg atgacgttga agatagtaag tatcataaaa atttaaaaga acttttttat
      361 tttgcactca acaatggacc aagtttgttc actggtatat cagtacctga tagtgataat
      421 ccattaatta tggcaaaaat ttacttaaca aaatggtgta ataaatacat cagggatagg
      481 aataacccag cattaaaaaa acctcttaaa acatttggcg aaaaagatgc tgctttaact
      541 actagagtag cagcaaatgc taatattgat gatcaaaaag tattgaatga ctacttgcgt
      601 ggacattttt tatatatgag cgctgagaac atgaacggaa gtattttaga ggaatattta
      661 gccgaagtgt tagaacctga aggttggatt tggtgcgccg gctctgtata tcgagctgtt
      721 gatttctgtt atttaggaac ctcaccaatt cttttacaag tgaaaaataa atacaataca
      781 gaaagcagtt cgagttcagc tataagagta ggaacaacaa ttagaaaatg gaatcgttta
      841 aataagtcga caaaaatttc tggtttggat agccctatcc ccaattggaa agcattaata
      901 gaaatgactg aagctagtaa ggaactggca gctaagttaa ctgaaaatag ttatttagct
      961 tatattaatg aaaaatccac acgagaatta tggacactcg atgactaaca atatccttat
     1021 tagtatctat ccacgataca tggaaaaaat tataaacaaa caaaaaaact ttgaatttag
     1081 aaatttctat cttacctcag atccttataa tatcttttgg atttacgaaa caatccccac
     1141 caaagcttta aagtataaat tgattgtaaa aaatccaatt actaaactta agaaaaatca
     1201 acactacttt ttaggagacg atgagtttga acaattagtt taaaag
//
LOCUS       DACCQI010000232         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCQI010000232 DACCQI010000000
VERSION     DACCQI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.244"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IVO76_002827"
     CDS             200..742
                     /locus_tag="IVO76_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430741.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IVO76_002828"
     CDS             832..>1246
                     /locus_tag="IVO76_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP7430742.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACCQI010000233         1239 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.245, whole genome shotgun sequence.
ACCESSION   DACCQI010000233 DACCQI010000000
VERSION     DACCQI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.245"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            852..1064
                     /locus_tag="IVO76_002829"
     CDS             852..1064
                     /locus_tag="IVO76_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430743.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          460 a          183 c          205 g          391 t
ORIGIN      
        1 tattaaatat tatacagata ataacggaat attatcatcg aaacagctaa caaaattgtc
       61 ataaaaaacg agatggttga tattagaact tgtgtttttg tttaggtcta gagataaaga
      121 gagacaattt ccaaatatat gtaatgaata caaaaagacg tttgtcgcgg attaccgtta
      181 caaacgtctt ttattgattt tccgagatct catcagtcgc catttggaat ggttcactcc
      241 ttatctagat tcgtttaatg ctcggactgc ttgttcgatt gtaataccaa acgcaacgtt
      301 attttcgttt tgtgcatcaa tcgccataaa acggttagaa gttgtatcaa aagctacgcg
      361 gtattgcata tttgtttttg taaacgtcat aaaattcatc tcctttgtaa tgtaatttag
      421 ttcttgaaag tttcctttaa actttcgaac ttataccaag tttagcatcg tacgtttttt
      481 gatgcaatta aaaagcattt atcattttcc ttatttattt tttcttaaat ggtttagcta
      541 ttttctttta atccgataag tagcccaatc atcaaggtta caaatactat tgcttgaata
      601 taaatcatat gctcacctct ttataaataa cttagcaata taaagaaaaa aacgcacgaa
      661 caacatagga atataaacaa acgtttttgt cacaacgtct aagatcgtgt taaaaaatag
      721 ttatctaaaa agcagaaaga tactcaaaat aaaaaataaa aaaatggata aaaacgtttt
      781 caaatacaag gtaatttgtt attataaaat aaagaaaaaa caaaataaaa ataaagaaaa
      841 gagggaatga aatggtaaaa gcagaagtag gaagatctta tatatgcaaa ccgatcggag
      901 tatctcatga actaatcggc tgtattgaac gtacatatat caacacggct gtcgtgtatg
      961 ttgaaagtta tgaccaacga gactatcaac ttatcaatga aagcaagtat cgcatgctcg
     1021 taaaattctg tgatattagc gcacctgctg aattggtttc ttaaacagta tttttgagaa
     1081 ttcgcaagaa ctcccttata atataaataa agacagaagg gagcgagaag atatgccaaa
     1141 aagaaaaagt tcagggaaaa tgggaattat tcttgcagtg atctctgcaa ttcctgctat
     1201 tttagctatt gttcgtgcga tgaaggaatc aagagaaag
//
LOCUS       DACCQI010000234         1234 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.246, whole genome shotgun sequence.
ACCESSION   DACCQI010000234 DACCQI010000000
VERSION     DACCQI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.246"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            34..531
                     /locus_tag="IVO76_002830"
     CDS             34..531
                     /locus_tag="IVO76_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7430744.1"
                     /translation="MNNQIALAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(568..1185)
                     /locus_tag="IVO76_002831"
     CDS             complement(568..1185)
                     /locus_tag="IVO76_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430745.1"
                     /translation="MYSQTIDSLNKRFGNRVLKGIEIGYIASEKDRIIDYLADKDYDL
                     KLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGL
                     SVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFS
                     VGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKRKRIMVPLPV"
BASE COUNT          430 a          208 c          216 g          380 t
ORIGIN      
        1 tttatagtat actatgtcaa aaggaggagt tagatgaaca atcaaatcgc actagcgtat
       61 ttagattatg atgatctaga tgctttacaa gttgtaagtg ttgtcagttt ttatgaatcg
      121 tttatcgatg gcgcagatcc acttgatatg caaggatatt tacagacaca attgacaact
      181 gaaatattgg cagtagaatt ggcacagtca accagtaaat ttattggtat taaggatcat
      241 caaatactca ttggttatat gaaagtcaat gatgaaaaag atgctataga gattcaacgg
      301 ctctatctgc tcaaagatta tcaaaacaaa gggttagggc aacggttact cgatgaagcg
      361 aatcgttatg ctaaaacgaa acaaaaacgt tatttacgtt tgactgttta tgaaaaaaat
      421 catgcaggta ttcgttttta tgaacgtcat ggatttaaaa aaatcgggat caaacatttt
      481 cctttaggaa aacaagatcg tatttgtccg attttagaaa aagaaatcta aaaaaacgtc
      541 ctcacttttg atgctgagga cgttttttta gactggtaat ggaaccataa ttcgctttct
      601 tttttgataa atcgctaatt ccgtcacacc ttttgctttt aacagagcaa aaagatcatc
      661 gaaatggaaa cggaaatgtt caaggtaatg cccatctgat ccaacactaa acaaggtacc
      721 accacgtgaa agatataaat cgatgacata ttcatataac gctaaattgt catacaagta
      781 agcgctctta gcatttaatt cgaatgccaa tttatttttg attacttgat ctagaatcgg
      841 taaaaactgc gcttcatatt gtttaaattc agcaacggat agaccaaata cccgaaaacc
      901 atagtcaaaa tgagcaaaga catctgcttc tataacaact agtgcttctg aaagttgtgc
      961 aaaatactga ggaatcacaa tagcaggatc catatctttg acttcgtcat ctaaataatc
     1021 aaattgacca ttatgatgca cactgagtaa ttttaaatcg taatctttat cggctaaata
     1081 gtcgatgatc cgatcctttt cagaagcgat gtaaccgatt tctatgcctt ttaaaacacg
     1141 attgccaaaa cgcttattta agctatcgat tgtttgactg tacatttcat aatcggggat
     1201 atcatcgact ttagtaacag gattggctaa gtca
//
LOCUS       DACCQI010000235         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCQI010000235 DACCQI010000000
VERSION     DACCQI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.247"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IVO76_002832"
     CDS             414..947
                     /locus_tag="IVO76_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7430746.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACCQI010000236         1204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCQI010000236 DACCQI010000000
VERSION     DACCQI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.248"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..1037)
                     /locus_tag="IVO76_002833"
     CDS             complement(<1..1037)
                     /locus_tag="IVO76_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7430747.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIK"
BASE COUNT          322 a          266 c          209 g          407 t
ORIGIN      
        1 ttctttatat ttattttgta ttccgttaca aactcgcgta cttggttagg ttccagagtg
       61 acatctccaa aactttcaag tcgctccgct cctggcagtt cttgagtctc aaatgtgata
      121 ccgccatgat ttactaattt attcccgcgc atttccggtc catcttctcc aaaatttgct
      181 gtaaaaatca cgattcctga agcatctgtt tttacttgga tcgatacttt ttcatctggg
      241 cttgtcaatt ccgcagcatt tagtaaacca gattgttttt tcaacaaaaa tggatgatcg
      301 ataccgccca ctatcattga ttggcgaaaa tcactggcaa agacctcacc taatttttta
      361 ggacgttgga agtcaaatgc tgtgccactt actgaaacta cttctcctgt aggcgttgta
      421 tctttattga gcgtagcaaa taatcggcta ttcaaccaaa gtatatgttc gtcgattgac
      481 tgtgctggat caccggttaa attgaaatag acatgatttg taggattgaa tactgttttg
      541 cggtcactga tcccttggac cgtcactcgc catgtatcat cgttcgtcag cgtataccgt
      601 acttctacat ctaaagttcc aggaaatccg ttttccatgt ctgggctagt ctgatagaaa
      661 atgaccgatg cttcgttttc accattgatg actgtataat gccactgctt ggcttcaaag
      721 ctaggtgcac caccatgtaa acaatgacca gtgtccgggt ctgtttttac ttcgaacgtt
      781 tcatcatcta atttgaatgt gccattcgta atccgtccag ccatacgtcc gattgaagca
      841 ccgatataag ggtctttttc tagatattct tttgcttcat caaaacctaa gatcaactct
      901 cgtttttcat tatcgattat tgtttgaaaa tgaacaaccc gtgccccaag atcactgaca
      961 gacaaagatg tcccattttg attcgtgatc gtaatcaagt gatagttttc gccaaaattt
     1021 tgttcattga tttccatgtt ccttgcaccc tcttttcttt catttgcact gattccagta
     1081 tacatggaaa ccgttttctt aacagcttat tccgtgagta acgagaaaaa atttactata
     1141 ttctaataaa agaccgtgaa aaaaaggttg tgacaaaaat attgtcacaa cctctttttc
     1201 cgaa
//
LOCUS       DACCQI010000237         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCQI010000237 DACCQI010000000
VERSION     DACCQI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.249"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1194)
                     /locus_tag="IVO76_002834"
     CDS             complement(<1..>1194)
                     /locus_tag="IVO76_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7430748.1"
                     /translation="PEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSGIRFLRA
                     RDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLITDDNPI
                     YFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGKKTPISL
                     PTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEVRFPGFT
                     GDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLELEEDKYA
                     NKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDKEKIKES
                     TNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLLKETKKG
                     FLQKM"
BASE COUNT          344 a          244 c          183 g          423 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggt
//
LOCUS       DACCQI010000238         1190 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCQI010000238 DACCQI010000000
VERSION     DACCQI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.250"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IVO76_002835"
     CDS             141..398
                     /locus_tag="IVO76_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430749.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IVO76_002836"
     CDS             complement(630..1043)
                     /locus_tag="IVO76_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP7430750.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          454 a          151 c          148 g          437 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta
//
LOCUS       DACCQI010000239         1183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCQI010000239 DACCQI010000000
VERSION     DACCQI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.251"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            98..1072
                     /locus_tag="IVO76_002837"
     CDS             98..1072
                     /locus_tag="IVO76_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7430751.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          421 a          143 c          201 g          418 t
ORIGIN      
        1 ttccgttagg aatgtgttat tcaataaatt ttataatcta gaaacatttt gaataaaaac
       61 tattagacaa atgatatttt acgaggagga ataaactatg tgtacgtcta ttacttatgt
      121 aacaagtgat cattattttg gaaggaattt tgattatgaa atatcttaca atgaagtagt
      181 tactgttact ccaagaaatt ataagttgaa ttttcgaaag gtaaatgatt tggatactca
      241 ttatgcaatg attggtattg ccgctggtat agctgactac cctctttatt acgatgcgac
      301 aaatgaaaaa ggattgagta tggctgggct aaatttttct gggtatgctg attataaaga
      361 aatacaagaa gggaaagaca atgtatctcc ttttgaattt attccttgga ttttaggaca
      421 atgctcaaca gtaggagaaa ctaaaaaatt gttaaaaaat atcaatttag caaatataaa
      481 ttatagtgat gaacttcctt tatccccttt acattggcta ttagctgata aagaaaaatc
      541 aattgtcatt gaaagtatga aagatggact tcatatatat gataaccctg tgggcgttct
      601 taccaataat ccttcatttg actatcaatt atttaattta aacaattatc gtgtcttatc
      661 gagtgaaact cctaaaaata atttttcaaa tcaaataagt ttgaatgcct atagccgcgg
      721 tatgggaggg ataggcttgc ctggagattt atcctcagta tctcgttttg ttaaagcgac
      781 ttttacgaag ctgaattctg tatctggaga ttcagagtca gaaagtatta gtcaattttt
      841 ccatatctta ggttcagtag aacaacaaaa aggtttgtgt gatgttggtg atggaaaata
      901 tgaatataca atttattctt cttgttgcaa tgttgacaaa ggaatctatt attatcgaac
      961 atatgaagac agtcaaatta ctgcaattga tatgaataaa gaagacttag atagtcataa
     1021 gttaattagt tatccaatta tagaaaaaca acaaattaaa tatataaatt agttaatgtg
     1081 ttgtgattga ttatttaata tagatataat acaaaaaggc aaagatattt tcaaaacatt
     1141 ttttcttgat gtgatatcct tgtctttttt gtaatgtttt taa
//
LOCUS       DACCQI010000240         1160 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCQI010000240 DACCQI010000000
VERSION     DACCQI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.252"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            56..>1160
                     /locus_tag="IVO76_002838"
     CDS             56..>1160
                     /locus_tag="IVO76_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7430752.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFR"
BASE COUNT          380 a          227 c          228 g          325 t
ORIGIN      
        1 tctctagtat gattaaatca ccacaaaata aacaactgag aggacgatac gctatatgaa
       61 tgattctatc aaaaaaatgc tgagaataac agagaaagat ttgatgatta cagaggtctc
      121 ttacgagacc cttcagaaga aaaagacgtt ggtcgtcgat gctgttctct cgcctactcc
      181 tcgtgcttgt agaagttgtg gttctactgt ggtagatgga aacgggaaag caattatagt
      241 gaaaaatgga aaaaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt
      301 tatgcgccta aaaaagcaac gctatacctg taaaaactgt cgcacccatt ggacggctca
      361 aagttatttt gtccaaccca gacattcaat cgcaaatcat gttagatata aaattgcttc
      421 tttactgact gaaaaagtat ctttatcttt tattgcgaaa agctgtcagg tatctttgac
      481 caccgttatt cgtacattga aagagtttaa aagctattta ccaaagcaat ctaagaagat
      541 cctccccaga gtattgatgg ttgacgaatt tcgttcgcat gcttccatag aagataagat
      601 gagctttatt tgcgcggatg gcgaaacagg aaaattaata gatgttttgc ctacgcgtaa
      661 attacctcga ttaacaagct atttcttagg ttgtaccaat ccagaagaag tagaattctt
      721 ggtgacagac atgaacgccg cctacttcca gctcaccaaa cgtgttctgc caaatgcgaa
      781 agtcgtgatt gatcggtttc atattgtcaa acacatgaat caagcgttca atgagttgcg
      841 tatccgtgaa atgaatgaac ttcgtaaagc cggacagaaa agccaggcag aaaaactgaa
      901 aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc aaccattatg aatacaaaac
      961 atggaaaagt ttccgagcac caaaataccc atttcttact gaagcaatga tgattgatcg
     1021 attgcttgag ttttctccgc ccctaaagga ggcgtatccc ttttttcatg aattagttga
     1081 agcctttcga gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac
     1141 gttagatgac agctttcggg
//
LOCUS       DACCQI010000241         1156 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCQI010000241 DACCQI010000000
VERSION     DACCQI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.253"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..139
                     /locus_tag="IVO76_002839"
     CDS             <1..139
                     /locus_tag="IVO76_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7430753.1"
                     /translation="RRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNN
                     I"
     gene            387..1121
                     /gene="erm"
                     /locus_tag="IVO76_002840"
     CDS             387..1121
                     /gene="erm"
                     /locus_tag="IVO76_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HAP7430754.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYTKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          449 a          173 c          179 g          355 t
ORIGIN      
        1 ccggagagaa cgagtgattc ggatttttcc aaatatcaat tccatcactc ggataattgg
       61 aacacttttg atggaaaaag acactgagtg gctggcttct ccacgaaaat atttagaatt
      121 taattcgaat aacatttagc accatgaacc tgtgcgagtc tcttctttct gaggagagac
      181 atcaagagtc gctgcgctac tgctcctgac ctctctcctc agaaagaaag gcatggcagt
      241 taggttcaca acgcaaaaca ttgtgctttt gatgaggttg aacttttaca caagattgtg
      301 gacttgactc aaccaaacaa aaaataagtg gttataatgg attgttaata taaacattca
      361 ttataacctc ataggagtgg gttataatga acaaaaaaaa tataaaagat agtcaaaact
      421 ttattacttc aaagcatcat ataaatgaaa ttttgagaaa tgtacattta aatacaaatg
      481 ataatattat tgagattggt tcagggaaag gtcatttcac gtttgaatta gctaaaaggt
      541 gtaattatgt aaccgccatt gaaatagatc ctaaattatg taggataact aaaaacaaac
      601 ttattgaata tgagaacttc caggttatca ataaagatat tttacaattt aagtttccta
      661 aaaataagtc atataagata tatggaaata taccctacaa tataagtaca gatataattc
      721 gaaaaattgt ttttgaaagc acagctacag aaagttattt aatagtggaa tatggatttg
      781 ctaaaaggtt gctaaataca aatcgttcac tagcactatt tttaatgaca gaagttgata
      841 tatccatatt aagtaaaatc cctagagaat actttcatcc aaaacctaga gttaatagct
      901 cgttaattgt attaaaaaga cacccttcaa aaatatcact caaagataga aaacaatatg
      961 aaaattttgt tatgaaatgg gttaacaaag aatacacaaa actattttcc aaaaaccaat
     1021 tttatcaagc cttaaaatat gcaagaattg acgatttaaa caatattagc tttgaacaat
     1081 tcttgtctct tttcaatagc tataaattat ttaatagata agttaagggg tgcataaact
     1141 gcatccctta acttat
//
LOCUS       DACCQI010000242         1134 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCQI010000242 DACCQI010000000
VERSION     DACCQI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.254"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(251..1132)
                     /gene="galU"
                     /locus_tag="IVO76_002841"
     CDS             complement(251..1132)
                     /gene="galU"
                     /locus_tag="IVO76_002841"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7430755.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          287 a          255 c          179 g          413 t
ORIGIN      
        1 actatgaatc ttctggcttg gctttcattt ctcttatgta atcattttct tttatacaac
       61 ttaaaaaaaa attaaaccat tagcataata ttttctgaat ctattcacga aagtcttttt
      121 ctatctttcg ttttccacga ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc
      181 gcaaatgacg cacaagctgc cgctttaata acaaatagcc tttcattctc agcaggccac
      241 ttatttccag ttaactttct gttcttagct ttttactcaa ctggataata tatgcactta
      301 atgaagtccc aatttccgaa tgttgaagtc catattcaat atttgtcttc atatatccta
      361 acttatcgcc tacgtcgtat ctttttcctt taaactctct cgcatagacc gcctctgtct
      421 ggttaagagt ctgaagtgca tccgttagct gtatctcgtt tcctgctcct ggttcttgag
      481 tttctaatat ctcaaatatt ttaggagtca gtagataacg tccgataatg gctaaattac
      541 ttggtgcttt tcccatttca ggtttttcta caaacccttt tactctgcaa cgaccctttt
      601 cgctatctgt tttaggatca ataattccgt atttagatgt ttcattttct ggtacacgca
      661 tgacggcaac agcggaagct tgtgtttctt cataatccat catcaattgt tgagtcaatg
      721 ggatatcatc tttcatcaaa tcatccccca gcatgacgac aaacggttca tttcctataa
      781 atgcttttgc ttgaagcacg gcatccccca gtcctcttgg atgagattga cggatgaagt
      841 gaagattgac ttgagtcgtt tcttcaacta aagcgagtaa ttctgttttt tccttttccc
      901 gcaaattgtt ttccaattcg atatttgcat caaagtgatc ttcaatgggt cttttttctt
      961 ttcccgttac gatcaagatg tcttcgattc ccgaatccaa tgcttcttcc acaataaact
     1021 ggatcgttgg tttatccacg attggaagca tttcttttgc catggctttt gttgccggca
     1081 aaaaacgtgt tcctagccca gcggcaggaa tgactgcttt tttgaccttc actt
//
LOCUS       DACCQI010000243         1130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCQI010000243 DACCQI010000000
VERSION     DACCQI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.255"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..1091
                     /locus_tag="IVO76_002842"
     CDS             <1..1091
                     /locus_tag="IVO76_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7430756.1"
                     /translation="NELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVLALFIPL
                     EQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNVGIEKNA
                     LTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAFEIGFKA
                     VKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISPNELVLF
                     DLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGELDQIAR
                     NVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYIPEKVGV
                     RIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT          342 a          247 c          248 g          293 t
ORIGIN      
        1 tcaatgaact acgtcaatgg atggcacaac accaagtcga attggcttat atcagcgacc
       61 ctggccacat cgcttacttt tccggatacg aaagtgatcc tcatgagcgt gtactagctc
      121 tttttatccc tcttgaacaa gatcctttcc ttttcactcc tgctttggaa gttgaagatg
      181 ctcaaaacag tagttgggcg tatgatgtcc gtggctattt agacagcgaa aacccttggg
      241 agatcatcgg aaaagaactt cgtgctcgct atggtcagcc tggcaatgta gggattgaaa
      301 agaacgcctt gactgtcgag cgcttcgatg ctatctctga tatcttgacg ggaacggatt
      361 cttacactga tctaacttcc atcatccaac gtctacaact gatcaaaact ctagaggaaa
      421 aagacaaact gatcgaagct ggtaaatggg ccgatgttgc atttgagatt ggctttaaag
      481 cagtgaaaga aggcgtgact gaacaagcga tcattgcaga aattgaatac caattgaaaa
      541 aacaaggtgt ttcacaaatg tctttcgaca cattagtcct tgcaggagca aatgctgcaa
      601 gccctcatgg tacaccagga aatacgaaga tttcaccgaa tgaacttgtg ttattcgatc
      661 ttggcgttgt ttggaatggc tattgcagtg atgctactcg tacaattgct tatcaaaaac
      721 caaccgactt ccaagaaaaa atctacaaca tcactcttga agcacagctt gccgcacaag
      781 aagccgtccg tccaggtgtg acagcaggtg aacttgatca aatcgcacga aacgtgatca
      841 acagctatgg ctacggcgaa tatttcaatc accgtttagg tcacgggatc ggaacaaccg
      901 tccacgaatt cccttccctt gtagaaggaa atgacttagt aatcgaagaa gggatgtgct
      961 tctctcttga accaggaatc tatattcctg aaaaagtagg ggtacgtatc gaagactgtg
     1021 tatatgtgac aagcgatggc tgtgtgccat tcacgactac accaaaagaa ttacttgttt
     1081 tagaaaaata agagtaaaaa aggtgagagc tagtccgctc ccaccttttt
//
LOCUS       DACCQI010000244         1117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCQI010000244 DACCQI010000000
VERSION     DACCQI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.256"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            1..384
                     /locus_tag="IVO76_002843"
                     /pseudo
     CDS             1..384
                     /locus_tag="IVO76_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          402 a          192 c          155 g          368 t
ORIGIN      
        1 cgaaaagaaa tctgtgatga ttttaaggct gtttatcaag ctagctcaaa ggaagaggca
       61 aatacctttt tagggagtat gattgagaag tagcaaaaaa cttatcctaa agtgacgcag
      121 tcactgataa aaaatcaaga tttattgact ttttatgagt ttccgcctgg tatccgccga
      181 agcatttact caactaattt aatcgaatct ttcaagaaac aaatcaagaa atacagccgc
      241 agaaaagagc agtttcaaaa tgaagaatca ctagagcgtt tcctagtatc catctttgat
      301 acatacaatc aaaaattctt aaatagaagc cataaaggct tccaacaagt gacggataca
      361 ttagcttcaa tgtttactga gtaacccatt attttgcaga aggacgagtt atttacacaa
      421 aattattgac gctccctcca aattacttca tcaaaacatt gatataattt ttattatatc
      481 agtatttatt cttgatacat aaatgagaaa ataaatactt taaatctatt gactacaagt
      541 agtcattaag tcttcataag atgtacaata tgacattaaa aaaataaaag cttacgacca
      601 tccgttaaat ctgacactaa ataatggact gctttctttt gtattagcag gttacgttga
      661 ctatgaatat tcaccagatt atttggagaa acctcttcaa cacgagaaca atgatattaa
      721 ttttagattt gtctacgagt caaaaagtaa aacatggtta gttgatcaat taagcacgat
      781 aaacacaagc ttccatgaat tacctaaacc tgaaaatgaa gttgagatgg tcattaaaat
      841 cttctaaaaa agaagcgttg aaggggaaat attcctttca acgctctttt ttatatgctt
      901 agaaacataa ataaattgta tactttcttt ttagccccta ttacttttca cgaaaaatat
      961 ctttttttcg acgctagcgt ttctctactt ctaatactgt acacagtttt tgtattattg
     1021 aaaaattact cattatttag cattaatcca catcgtttta tcaatcgacc ttcatactaa
     1081 actactttaa agtcatcttc cttctcgttt ttcatta
//
LOCUS       DACCQI010000245         1090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCQI010000245 DACCQI010000000
VERSION     DACCQI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.257"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            58..912
                     /locus_tag="IVO76_002844"
     CDS             58..912
                     /locus_tag="IVO76_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7430757.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
BASE COUNT          418 a          143 c          180 g          349 t
ORIGIN      
        1 taataatata aacataataa aacaaaaatt atccaaatct aacataggag ggtggatatg
       61 gaaaattcta tatatttagt aacagaacct aacactcagc tgaaagattg ttactttaat
      121 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
      181 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      241 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      301 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      361 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      421 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      481 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      541 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      601 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      661 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      721 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      781 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      841 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      901 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      961 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
     1021 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
     1081 ataagttaca
//
LOCUS       DACCQI010000246         1085 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.258, whole genome shotgun sequence.
ACCESSION   DACCQI010000246 DACCQI010000000
VERSION     DACCQI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.258"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>1085
                     /locus_tag="IVO76_002845"
     rRNA            <1..>1085
                     /locus_tag="IVO76_002845"
                     /product="23S ribosomal RNA"
BASE COUNT          332 a          215 c          318 g          220 t
ORIGIN      
        1 ataagggcgc acggtggatg ccttggcact aggagccgat gaaggacggg actaacaccg
       61 atatgctttg gggagctgta cgtaagctat gatccagaga tttccgaatg ggggaaccca
      121 acatctttaa taggatgtta cgattgtgtg aatacatagc acattcgagg tagacgcaga
      181 gaactgaaac atctaagtac ctgcaggaag agaaagaaaa ttcgattccc tgagtagcgg
      241 cgagcgaaac gggaaaagcc caaaccagca agcttgcttg ttggggttgt aggactccaa
      301 tatggtagtt ctttcagata gtcgaatgac ttggaaaagt cagtcaaaga gggtaaaaac
      361 cccgtagacg aaatgtggaa gacacctagg aggatcctga gtacggcgga acacgagaaa
      421 ttccgtcgga atccgggagg accatctccc aaggctaaat actccctagt gaccgatagt
      481 gaaccagtac cgtgagggaa aggtgaaaag caccccggaa ggggagtgaa atagaacctg
      541 aaaccgtgtg cctacaacaa gtcaaagccc gttaatgggt gatggcgtgc cttttgtaga
      601 atgaaccggc gagttacgat tgcatgcgag gttaagttga agagacggag ccgcagcgaa
      661 agcgagtctg aatagggcgt ttgagtatgt agtcgtagac ccgaaaccat gtgatctacc
      721 catgtccagg ttgaaggtgc ggtaaaacgc actggaggac cgaacccacg tacgttgaaa
      781 agtgcgggga tgaggtgtgg gtagcggaga aattccaaac gaacttggag atagctggtt
      841 ctctccgaaa tagctttagg gctagcctcg gaattgagaa tgatggaggt agagcactgt
      901 ttggactagg ggcccatctc gggttaccga attcagataa actccgaatg ccattcattc
      961 atatccggga gtcagactgt gagtgataag atccatagtc gaaagggaaa cagcccagac
     1021 caccagctaa ggtcccaaaa tatatgttaa gtggaaaagg atgtggggtt gcacagacaa
     1081 ctagg
//
LOCUS       DACCQI010000247         1074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCQI010000247 DACCQI010000000
VERSION     DACCQI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.259"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            116..379
                     /locus_tag="IVO76_002846"
     CDS             116..379
                     /locus_tag="IVO76_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7430758.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            379..651
                     /locus_tag="IVO76_002847"
     CDS             379..651
                     /locus_tag="IVO76_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7430759.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            728..1057
                     /locus_tag="IVO76_002848"
     CDS             728..1057
                     /locus_tag="IVO76_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7430760.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          407 a          134 c          212 g          321 t
ORIGIN      
        1 aaacggataa cactttacaa tgctgttttg gtactccaat ttaaacaaaa caatcgaatt
       61 tacgtactta attaagtacg ttagaatgga gatataaatt agagggggag tagatatggc
      121 aaaaacagtc ttaaatccta gctcagcacg gaaaaatttt tataagttgt tgaaagatgt
      181 aaatgagaat catacggaaa ttgaaatcat tagtgagcgc agtgaaaata atgcggtgct
      241 tattggtttg gatgattgga aagcaatcaa agaaacgcta ttacttgagc aaataggcac
      301 tttgggtgtt gttcgtaagc gagaaaaaga tgatagtgga tttactaatg ttgatgaaat
      361 tgattgggat aacttataat gaataactat tctgtgatga ttaaaaactc ggctaaagct
      421 gatttaaaga aaatcaaaca gtctaatttg aaacctcaat ttgagaaggt tattcagact
      481 ttaaaggaag atccctatct ccctacccaa tcatttgaga agttaaaacc tacacatgaa
      541 ggtaggtatt ctagaagatt gaatagacaa caccgggttg tttataaggt agatgaagat
      601 gaacacgtgg ttgaaattta ttcagcaagg acgcattacg agtcacagta attacttgta
      661 aatacattat ctcgtggtat aatgtattta ctaagtaatt acatactgtt agaaatgagg
      721 gatttaaatg attacaaaaa caagaaaaca aggcaattca atcatgttga cagtacctaa
      781 agattttaat gttcctaatg gagtcgaagt tgaagcaaag ttggtagaga atggtattct
      841 ttatgaattt gttgaaccac aaaaagaatt ttatgatttt agcgaagata ttttgtctga
      901 tataattgca gaaggctacg ataaagatga gattcttgta gaatttaaga atagaaaaaa
      961 caaaatgcat tcttccttta gagatattgc tgaagataca cttactaatt ctaaagtaat
     1021 gactaaagag gagttggcaa cagaaattgg cttatgaaat cgttccttca aaac
//
LOCUS       DACCQI010000248         1073 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCQI010000248 DACCQI010000000
VERSION     DACCQI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.260"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..884
                     /gene="rlmD"
                     /locus_tag="IVO76_002849"
     CDS             <1..884
                     /gene="rlmD"
                     /locus_tag="IVO76_002849"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7430761.1"
                     /translation="YIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGH
                     YSHEMMVVLVTRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYG
                     RDYIEDQLLGKTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIG
                     LSLADRVDKVYGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSV
                     IVVDPPRKGLDHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDL
                     FPQTHHIELVASFVKGE"
     gene            887..1015
                     /locus_tag="IVO76_002850"
     CDS             887..1015
                     /locus_tag="IVO76_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7430762.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          410 a          170 c          211 g          282 t
ORIGIN      
        1 tttatatcca agatcctgtc atcgatcaag caatcaaagt agtcagagat atcttgcaac
       61 ggttccaagt acgaggatat aatgaagaaa aaaatgaagg acagcttcgt cacatcatta
      121 tccgtcgtgg tcattattcc catgaaatga tggtcgtttt agttactcgt aaagaaaaat
      181 tctttaaatc caaggaaatt gctgaagcta ttcatgaaga attgccagaa gtggtttcgg
      241 tcatccaaaa tatcaatgaa gaaaaaacaa atgtgatttt aggagacaaa gaaaaagttt
      301 tatatggcag agactatatc gaagatcagc tattaggaaa aacctatcga atctcagcaa
      361 agtcatttta tcaagtaaat actcaacaag cagagtatct gtaccaaaca gccatcgagt
      421 ttgccgagtt agataaaaaa gacattgtga tcgacgctta ttcaggaata ggtacgattg
      481 gcttgtcttt agcagacaga gtggataagg tgtatgggat ggaagttatt ccagaagcaa
      541 ttgaagatgc acagttcaat gctttaaata ataaaatcga aaatgttcat tatgaagtcg
      601 gaaaagcaga aaaagttatg aaaaaatggc aagaaaaagg gatcaaacca tctgtgatcg
      661 tagttgaccc tccacgaaaa ggattagatc atcattttat tgaatcagca atcgaaatgt
      721 caccagaacg aatcgtctat atctcatgta atccggcaac ttttgcaaga gatgctaagt
      781 tatttgcaga agcaggttat gccgtgaaga aagttcagcc tgtagatctt ttcccgcaaa
      841 cccatcatat cgaattagtc gcttcattcg ttaaaggaga ataaaaatgg accaaaaaga
      901 aaaaatgaaa gaattgatca aaaagaaaca aggtggaggc cgatccaaac aaatggaaac
      961 acctaagaat gatcgtaaaa acatgagaaa aggccctaaa atctttaata aatagacgtg
     1021 ctaaagaaag actaagaagt gttaactttt gttattgttt cttagtcttt tta
//
LOCUS       DACCQI010000249         1058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCQI010000249 DACCQI010000000
VERSION     DACCQI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.261"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IVO76_002851"
     CDS             complement(36..992)
                     /locus_tag="IVO76_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430763.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACCQI010000250         1038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCQI010000250 DACCQI010000000
VERSION     DACCQI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.264"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>1038
                     /locus_tag="IVO76_002852"
     CDS             <1..>1038
                     /locus_tag="IVO76_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7430764.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKH"
BASE COUNT          361 a          204 c          187 g          286 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacacc
//
LOCUS       DACCQI010000251         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCQI010000251 DACCQI010000000
VERSION     DACCQI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.265"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1035)
                     /locus_tag="IVO76_002853"
     rRNA            complement(<1..>1035)
                     /locus_tag="IVO76_002853"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACCQI010000252         1022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCQI010000252 DACCQI010000000
VERSION     DACCQI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.266"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(175..>1022)
                     /locus_tag="IVO76_002854"
     CDS             complement(175..>1022)
                     /locus_tag="IVO76_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7430765.1"
                     /translation="DDPNWRMFFGILNNLYPTHPLHIDIAGTVESIDKITAQDLYTCY
                     RTFYQPSNMVLFVVGKMEPEKLMKLIRENQEAKNFPPKQEIVRYFPENTKEIIKQSAL
                     EAAITRDKFVLGIKGLDTLPQEGTELLRYKTAINLLFQMILGNTSRNYLAMYNQGIID
                     DSFGFEFSLDREFHFADFSGDTDEPEKAAEKVKEIILGFADDPEVSETNLDLLKKKML
                     GQYFQSLNSIEYIANQFTQSLFGDRTLFDLPEIIDSIQMKDVLAAGEAFISEEAFSEF
                     YMRPK"
BASE COUNT          290 a          195 c          177 g          360 t
ORIGIN      
        1 attacttcgt ttgattttat tttaatataa gttgattaaa agagagcatg atttgtttta
       61 ttttctcact aatttttcca acacaactaa gaataccata aaagctgatt ttggaggagg
      121 ttcctactac aagtctaaaa cgaaatttaa gacttttcct cataaaatac tggtttattt
      181 tggtctcatg taaaactcgc tgaaggcctc ttcacttata aatgcttccc cagctgccag
      241 tacgtctttc atttggatcg aatcgatgat ttcaggaaga tcaaataaag ttcgatcgcc
      301 aaataaactt tgcgtaaatt gattggcaat atattcgatc gaatttaatg attggaaata
      361 ttgtccaagc attttcttct tcagcaaatc gagatttgtt tctgacactt ccggatcatc
      421 agcgaatcca agaatgattt ctttgacttt ttctgctgct ttctctggtt cgtccgtatc
      481 tccgctgaag tccgcaaagt gaaattcacg atccaagctg aattcaaaac caaagctgtc
      541 atcgataatt ccttgattgt acatcgctag atagtttcga gaagtattgc ctaaaatcat
      601 ttggaacaac agattgattg ctgttttata gcgtaacagt tctgttcctt cttgcggcaa
      661 tgtgtctaat cctttaatac caagtacaaa tttatctcgt gtaatggctg cttccaatgc
      721 actttgtttg atgatttctt ttgtgttttc agggaaataa cgtacaattt cttgctttgg
      781 agggaaattt tttgcttctt ggttttcccg aataagtttc atcaatttct caggctccat
      841 cttaccaact acaaataaaa ccatattgct tggctgataa aacgtccgat agcacgtata
      901 caagtcttga gctgtgattt tgtcgatact ttcaaccgtg cccgcaatat cgatatgcaa
      961 tgggtgtgta ggatacaagt tatttagtat gccaaaaaac atccgccagt tagggtcgtc
     1021 tt
//
LOCUS       DACCQI010000253          981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCQI010000253 DACCQI010000000
VERSION     DACCQI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.267"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            199..383
                     /locus_tag="IVO76_002855"
                     /pseudo
     CDS             199..383
                     /locus_tag="IVO76_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
     gene            417..848
                     /locus_tag="IVO76_002856"
     CDS             417..848
                     /locus_tag="IVO76_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP7430766.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT          337 a          150 c          206 g          288 t
ORIGIN      
        1 atgaaaagag acaaaaagaa ttgcagtaac caaagcagaa gtacggcaaa agtgaatcca
       61 ggattatgtg tattgcgtta gatttattga ttgcgcaaga aaaagcagga tttgacatac
      121 cagcactcaa aaagtgatcg cactttgcgc acgtgttaat acgtgttata attattttgt
      181 aaggaggtat agtgcacaat gccacttacg ggaaaagaaa tgcttaagtt attgaaaaaa
      241 atggctgggt tgagcgaagg caagagggat ttcatcatca tctttataag gatggtgtta
      301 gaatcactgt tcctgttcat gccaatcaag atcttggccg aggacttgag cgaaaaattt
      361 taaaggatgc gggattgaaa taatctcgca ttcgattaaa gaaaggattg actgaaatgt
      421 taagtgagcc tttagcaaaa acctatcctg ctatttttag cccagaagaa ggtggtgggt
      481 actttattga atttcctgat gttcaaggtg cttacacagg aattaacgaa gacgatatat
      541 cttatggaat tgctatggca caagaggtgt taggtatggt tttagcagat tatattgagc
      601 atgaagatct acttccagaa ccaacaccaa tcaataagat ttctgttgaa gatgattctt
      661 ttactacttt aattcgtgta gatgttgcca aatatttgaa agacacagaa cttgtcaaaa
      721 aaaccttaac aattcctcaa tgggcggata agctaggcaa aagggctggc ataaattttt
      781 cagtccttct aacagaatca attgcagata aagcagataa cattcttcgt tctggaagaa
      841 ataattagaa ctcaaaaggg tcagaaactt aaaaatcttt ggcttctttt tttactacaa
      901 agatggcttc tttaaattat ttgcctgatt gatttaaaaa aattgcgcaa agactacgag
      961 ctaattaagt tgctcgtggt c
//
LOCUS       DACCQI010000254          955 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCQI010000254 DACCQI010000000
VERSION     DACCQI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.268"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>955
                     /locus_tag="IVO76_002857"
     CDS             <1..>955
                     /locus_tag="IVO76_002857"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7430767.1"
                     /translation="TIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQLASTY
                     NQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEFLISQ
                     FASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTH
                     PDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVM
                     NPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGV
                     LFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIV"
BASE COUNT          299 a          181 c          209 g          266 t
ORIGIN      
        1 ccacaatcgt tgatattttg ggatatgcaa ttgcaccaga gtatcttttc aacgtcttag
       61 cggatcaagc aaaacaagca acgttccaat tgaacgatct aaacaaagcc tttgtgcaat
      121 tggcatctac ctacaatcaa tttaatgggt tatttgatga tgtggatctc caatcaaaaa
      181 aactaggaac agatgagcaa caacgaaatg tgacgattac cgaagtcatc aagaaattga
      241 atgatgtaga tgttttagaa cacgatgggg acgtgatcgg ggatgcgtat gagtttctga
      301 tcagtcaatt tgcttcagaa gcagggaaga aagccggaga attttacaca cctcatatgg
      361 tttctgatat gatggcgcaa atcgtaacgc tcgatcaaaa ggaacgacga ttctttagtg
      421 tttttgatcc aacgatgggt tcaggttcat tgatgttaaa cgtacgtaac tacttaacac
      481 acccagataa cgttaaatat catgggcaag aactaaatac aacgacatac aatctagcaa
      541 aaatgaactt gattttacat ggtgtcgacg ccgaagaaat gaatctccgc aatggggata
      601 cgttgaataa ggactggcca acagatgaac cttacacatt tgatgcagta gtaatgaacc
      661 caccttattc ggctaattgg tcagcagata cgactttctt agatgattct cggtttaatc
      721 gttatgggaa attagcgcca aaatccaaag cagattttgc gtttcttctt catgggttct
      781 atcatttgaa ggaaacaggt accatggcca tcgtcttacc acacggagtt ttattccgtg
      841 gcgctgcaga aggggtcatc cgtcaaaaat tactagaaga tggcagtatt tatgctgtga
      901 tcggtatgcc tgcgaacttg ttctttggga catcgattcc aacgacggtg attgt
//
LOCUS       DACCQI010000255          948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCQI010000255 DACCQI010000000
VERSION     DACCQI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.269"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(134..424)
                     /locus_tag="IVO76_002858"
     CDS             complement(134..424)
                     /locus_tag="IVO76_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430768.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(542..802)
                     /locus_tag="IVO76_002859"
     CDS             complement(542..802)
                     /locus_tag="IVO76_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430769.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
BASE COUNT          393 a          153 c          132 g          270 t
ORIGIN      
        1 ttactatgaa taacataata agaaagtaaa gtggattgat ttgagatctt tttatacttc
       61 aaaaaagata acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat
      121 acattttaat acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag
      181 agatcataag ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc
      241 atatttcttc aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc
      301 agcaccattt ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa
      361 gtaaaaacaa agctttactc ataactaaca aaataccagc tccttttatt tttttctccc
      421 ccacattcaa agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat
      481 gaataacagc tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag
      541 tttatttttt tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc
      601 cgatttcgta gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat
      661 ggataaaata gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata
      721 agtaaaagaa agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga
      781 caatcaaatg atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa
      841 aaacaaaata tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat
      901 ataaaaatcg cccctcattc atcgagaggc gataagttct aatttaac
//
LOCUS       DACCQI010000256          900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCQI010000256 DACCQI010000000
VERSION     DACCQI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.272"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            466..807
                     /locus_tag="IVO76_002860"
     CDS             466..807
                     /locus_tag="IVO76_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7430770.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          325 a          104 c          211 g          260 t
ORIGIN      
        1 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
       61 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
      121 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
      181 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
      241 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
      301 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
      361 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattaat
      421 ataaagcata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
      481 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
      541 ggatttgtga cgtttagaag gggcggaaat tgtaaaggat tagatgtttt agcattagac
      601 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
      661 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
      721 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
      781 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
      841 tagaaggagt cacgacgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
//
LOCUS       DACCQI010000257          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCQI010000257 DACCQI010000000
VERSION     DACCQI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.273"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IVO76_002861"
     CDS             <1..888
                     /locus_tag="IVO76_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP7430771.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACCQI010000258          826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCQI010000258 DACCQI010000000
VERSION     DACCQI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.274"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..409
                     /locus_tag="IVO76_002862"
     CDS             <1..409
                     /locus_tag="IVO76_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7430772.1"
                     /translation="EVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPM
                     DRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDE
                     IEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            481..>826
                     /locus_tag="IVO76_002863"
     CDS             481..>826
                     /locus_tag="IVO76_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430773.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          350 a           98 c          137 g          241 t
ORIGIN      
        1 agaagttata cgtgaaaatg tattacaaag agaattaaat actagagccg gttatgtgta
       61 tgtaattagt aatattgggt cttttggtga agacatatat aaaattggta tgactagaag
      121 attagaacct atggatagaa taaaagaatt gagtagtgca tcggttccat ttgaatttga
      181 cgttcatgcg atgatttttt cagaggacgc tccgtctttg gaaactaaat tgcataatca
      241 ttttagaaaa caagaagtta ataaaattaa tcaaagaaaa gaatttttca aagtttcact
      301 agacgaaata gagaaagttg ttttagaaaa ttataatgga acagtaacat ttacaaaatt
      361 agctaaagct gaacaatata gacgatcttt agaattgtca aaagattaaa tatataaaaa
      421 gccttcgggc tttttctttt aacaattaat aggaacgtat attcgaaagg aatagaaata
      481 atgtggttag aaaaaataaa tgacaaaaaa tttaagtaca ccgaacgtta tacggatccc
      541 ttaactgaaa aaaagagaaa agtgtccgtt actttatcta gcaattcacg ccaagcttgg
      601 aatcaagcta acttactttt gaatgaaaaa atcgcagaaa aaataaaacg aaatgaagaa
      661 ttaccactaa cttttggaga attaaaaact aaatgggatg aaaaatacaa gccaactgta
      721 aaagaaagtt cctatcgaac aactcaggtt tatctatcat taatttcaaa atatataaaa
      781 gatgatgtac ttgtaaaaaa cgttaatagt aatcttatac aagata
//
LOCUS       DACCQI010000259          812 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCQI010000259 DACCQI010000000
VERSION     DACCQI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.275"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..>812)
                     /locus_tag="IVO76_002864"
     CDS             complement(<1..>812)
                     /locus_tag="IVO76_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7430774.1"
                     /translation="KISKADPESGYYVKGEREKQFAYSVHTSCDDNGFILSTIITPRN
                     IHDSQVAFQLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKE
                     LLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNCPLLNQCTISKN
                     HQRVITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKHGMRWTKYRGL
                     EKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSL"
BASE COUNT          222 a          167 c          151 g          272 t
ORIGIN      
        1 ccaaactcgt aactcgagct tggaacagct ttttatctac ttcgtttcga atttttgaac
       61 aaaaaaataa aggctccttg cctttccata gccaggtggc taatttcttt aaattcattg
      121 cagcaaacac tagcatcgta tgcgtcgcga ctttttccaa gcctcgatac ttggtccaac
      181 gcatcccatg cttttctttt gcatccgcaa atattcgttc aatcgtttgt ttccgttttt
      241 tgtaaattga tttattcaga tctgttaggc gaagatgttc cacttcatcc atcagatctc
      301 cccaaacatg tctcgtgatg actcgttgat gatttttact tatggtacat tggtttagca
      361 gaggacagtt cacacattct tttggattgg atttgtattc tcggtaccct tctcgagtaa
      421 ccgttgaaaa agcgagcatt tgatcttgcg gacaaatata acaatcaaaa tactcgtcat
      481 agagaaactc attttttgat aatagctcct ttttcccttt gggccttgaa taaggcaaac
      541 aaggtcgaag gttcaaatga gtcaaaaagt gaacaaactt aggtgtctta tacccagcat
      601 cagcgacaac acaattgatt tcaggaaaaa gtctcttcga ttgtttgacg agctgaaagg
      661 cgacttgact gtcatgaata tttctaggag tgatgattgt gctaagaata aatccattgt
      721 cgtcgcatga agtatggaca gaataagcaa attgtttttc tcgttcgcct ttgacgtagt
      781 agccgctttc cggatcagct ttacttattt tt
//
LOCUS       DACCQI010000260          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCQI010000260 DACCQI010000000
VERSION     DACCQI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.276"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IVO76_002865"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IVO76_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7430775.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACCQI010000261          804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCQI010000261 DACCQI010000000
VERSION     DACCQI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.277"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
BASE COUNT          266 a          152 c          111 g          275 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc acttgactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttctcaagtg gaatcaactc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atgg
//
LOCUS       DACCQI010000262          788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCQI010000262 DACCQI010000000
VERSION     DACCQI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.278"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..780
                     /locus_tag="IVO76_002866"
     CDS             <1..780
                     /locus_tag="IVO76_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7430776.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          338 a          107 c          102 g          241 t
ORIGIN      
        1 gatatgctag attactacta ttttgaaaaa aatttatcat ataattatgt aatacattta
       61 aaaactttta caggaatgat ttttaaattc gccaaccgtc gatatggatt aaaatataat
      121 cccgtaaatg gggtttcttt agcaagaaaa cctaagacaa acgaagaaat aaaaaaagaa
      181 aaagatggtt atttatcaaa agaagaagtt caaatgattg ctagtaaaca aaaaagttca
      241 catcaacagt cacgttattc tttaattact aaatttttgt ttttaactgg actacgttat
      301 ggtgaattga tatctttgac tgaatctgat tatgatggta ataaaattac ggtttctggt
      361 acttatgatt acgagttaaa aattaaaaca acaactaaaa atacaggttc ttatagaacc
      421 attgaattat caagtaatgc taaagaaatc atagatcaat taattgaaga aaacaaatta
      481 attaagaata ataaagataa atacattttc atctctaaaa atggtaaccc gatatctatt
      541 caagcatata atcaatcttt aagagcagta tccaaagaat taaacataga taaaaaagtt
      601 tcttctcaca tgttaaggca ttcccacatt tctctactta cagaattagg aatcccatta
      661 aaagccatta tggatcgtgt aggacatgaa gattcaaaaa caacattaaa gatttatact
      721 catacaacta aaaatatgca aaatcaacta gtagaaaaac taggaaaaat agaaatttaa
      781 attgcccc
//
LOCUS       DACCQI010000263          748 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCQI010000263 DACCQI010000000
VERSION     DACCQI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.279"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(275..>748)
                     /locus_tag="IVO76_002867"
     CDS             complement(275..>748)
                     /locus_tag="IVO76_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430777.1"
                     /translation="RKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDG
                     EQSFSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILR
                     LSCNQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          212 a          162 c          128 g          246 t
ORIGIN      
        1 tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa aagacgattt
       61 tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc agtaaaagca
      121 tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga aattgatata
      181 gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga acttcttcta
      241 tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt acttttgtca
      301 gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa tcaagctttt
      361 cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc caccgctttt
      421 cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct tttattggca
      481 aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt gttcgactgt
      541 ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg atgaacatct
      601 cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc aaaaagcaaa
      661 acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta taagaaaaag
      721 aaaggtggta ctcgccaaac cactttct
//
LOCUS       DACCQI010000264          624 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCQI010000264 DACCQI010000000
VERSION     DACCQI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.280"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            76..519
                     /locus_tag="IVO76_002868"
     CDS             76..519
                     /locus_tag="IVO76_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7430778.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
BASE COUNT          214 a           93 c          139 g          178 t
ORIGIN      
        1 gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg aatgaaaaaa
       61 aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg tgtttcaacg
      121 gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat cttcttcagt
      181 cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat catgctgttg
      241 ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc cgctagtctt
      301 cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa cgacacagct
      361 tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa acgagctgat
      421 ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca agaagaagag
      481 atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt ccaaataaaa
      541 aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac ttctggaacg
      601 ccgtgaataa ggggttgtga aaaa
//
LOCUS       DACCQI010000265          579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCQI010000265 DACCQI010000000
VERSION     DACCQI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.281"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            108..>579
                     /locus_tag="IVO76_002869"
     CDS             108..>579
                     /locus_tag="IVO76_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP7430779.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVE"
BASE COUNT          261 a           68 c           83 g          167 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaa
//
LOCUS       DACCQI010000266          544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.282, whole genome shotgun sequence.
ACCESSION   DACCQI010000266 DACCQI010000000
VERSION     DACCQI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.282"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(21..106)
                     /locus_tag="IVO76_002870"
     tRNA            complement(21..106)
                     /locus_tag="IVO76_002870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70..72),aa:Leu,seq:taa)
     gene            complement(123..194)
                     /locus_tag="IVO76_002871"
     tRNA            complement(123..194)
                     /locus_tag="IVO76_002871"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Gly,seq:gcc)
     gene            complement(206..278)
                     /locus_tag="IVO76_002872"
     tRNA            complement(206..278)
                     /locus_tag="IVO76_002872"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Thr,seq:tgt)
     gene            complement(296..377)
                     /locus_tag="IVO76_002873"
     tRNA            complement(296..377)
                     /locus_tag="IVO76_002873"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Leu,seq:tag)
     gene            complement(385..457)
                     /locus_tag="IVO76_002874"
     tRNA            complement(385..457)
                     /locus_tag="IVO76_002874"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(422..424),aa:Lys,seq:ttt)
     gene            complement(461..533)
                     /locus_tag="IVO76_002875"
     tRNA            complement(461..533)
                     /locus_tag="IVO76_002875"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(498..500),aa:Val,seq:tac)
BASE COUNT          138 a          155 c          132 g          119 t
ORIGIN      
        1 ctatgggcgc atatttgaaa tgccgaggac cggaatcgaa ccggtacggt gatcactcac
       61 cgcaggattt taagtcctgt gcgtctgcca gttccgccac cccggcgcga ttgctttggc
      121 aaagcggaaa acggggttcg aacccgcgac ccccaccttg gcaaggtggt gctctaccac
      181 tgagctattt ccgcgtgttt aaagatgccg gctaaaggac ttgaaccctc gaccctctga
      241 ttacaaatca gatgctctac caactgagct aagccggcat gtaaaactta aattatgcgg
      301 gtgaagggac ttgaaccccc acgccgtaag gcgctagatc ctaaatctag tgcgtctgcc
      361 aattccgcca cacccgcaaa aatatgagcc gtacagggct cgaacctgtg accctctgat
      421 taaaagtcag atgctctacc aactgagcta acggctcata tggaggttaa cgggatcgaa
      481 ccgctgaccc tctgcttgta aggcagatgc tctcccagct gagctaaacc tccatggata
      541 actt
//
LOCUS       DACCQI010000267          531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.283, whole genome shotgun sequence.
ACCESSION   DACCQI010000267 DACCQI010000000
VERSION     DACCQI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.283"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(66..287)
                     /locus_tag="IVO76_002876"
     CDS             complement(66..287)
                     /locus_tag="IVO76_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7430780.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          183 a           88 c          101 g          159 t
ORIGIN      
        1 tttttttgtt ttcattaaga cgtattacct aagagttcca tgactttagt ctggtagcgt
       61 gtcagctaac caaaaaagag aaaaagaaaa gtaaaaaatt cgactttctt ttttctatag
      121 tatataaatt tttgcatata gataaagatc aagataccga taacggaagc tagattgtaa
      181 tctattttag tgacagtcag gtatagccaa gtaactagaa gatcagtgga acctgcacca
      241 taaaagtttg agtttgtcgg tccgttgaca gttaaaagaa aaatcacgtt gaagttattg
      301 atatttccga ttacgccgct tcgatttggt cacgatccaa attttggata atccctgtag
      361 aaattagcat agtagctggg ataccaattc acatattgac aaagataaca gagactttta
      421 cataaaacgg atcaatcagg aaaggaattg ctcgatccag ccccagtttt ccaacaatgt
      481 gttaactggc ctagcgtcat tcagcaaatt atgcatgatg aggagcgaaa t
//
LOCUS       DACCQI010000268          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.284, whole genome shotgun sequence.
ACCESSION   DACCQI010000268 DACCQI010000000
VERSION     DACCQI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.284"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            29..>517
                     /locus_tag="IVO76_002877"
     CDS             29..>517
                     /locus_tag="IVO76_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP7430781.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT          190 a           95 c           85 g          147 t
ORIGIN      
        1 accaattcac agaaaagagg acttccctat gaatgatttt actacagaaa ttgtgcaaac
       61 tctagtcact aaaggcgatt taaatgaatt attccgttcg cacttagaaa aagcgataaa
      121 cacactccta cggactgaat taacggcttt tttagattac gaaaaatatg atcgcactgg
      181 ttttaattca ggtaattcga gaaacggttc ttactttcga tcaatcaaaa ccgaatatgg
      241 tgaattaaca ttggaaatac ctagagatcg taatggtgag tttaaacaac aaactttacc
      301 agcctacaaa agaacaaacg atacattgga aaccactatt atccatttat tcgaaaaagg
      361 tgttacgatg tctgaaattg ctgatttgat cgaaaaaatg tacggtcatc actatactcc
      421 acaaaccatg tccaacatga ctaaagttct gactgaagaa gtaaatgcct ttaaatccag
      481 agccttaaat gataagtatg tcgctatttt tatggac
//
LOCUS       DACCQI010000269          498 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCQI010000269 DACCQI010000000
VERSION     DACCQI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.285"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(54..>498)
                     /locus_tag="IVO76_002878"
     CDS             complement(54..>498)
                     /locus_tag="IVO76_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HAP7430782.1"
                     /translation="LEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKDKP
                     ILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDAAL
                     KIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT          144 a           91 c           80 g          183 t
ORIGIN      
        1 tgacaaataa tacaatgtaa aaaattctgt tttgcgggat cgattttatc attttattgg
       61 tcaagatttt tccatttcca atccaatact tctggcagat caataccatt ttcacgaata
      121 tagttttgat gttcttgtaa tttctgatcc attttttccg caaaatcttc tgcttgactt
      181 cctttgattt ttaaagctgc gtcttttgcc aaatgataac gatcaagttc atttactaca
      241 cgcatgtcaa atggtgtcgt gatatctcca ttttctttgt agccatgaat cattaaatga
      301 tggttatgac gatcaaagaa aatcgtgcgg accaatgttt cataaccgtg gaaagcaaat
      361 aagatcggtt tgtcttttgt aaaatattga tcaaattctt catcgctcaa cccacgcgga
      421 tcttgacttg gatgacgtaa tttcaataaa tctacgacat tgatgaaccg aatttttagt
      481 tttgggaatt cttccagc
//
LOCUS       DACCQI010000270          491 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.286, whole genome shotgun sequence.
ACCESSION   DACCQI010000270 DACCQI010000000
VERSION     DACCQI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.286"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            6..398
                     /locus_tag="IVO76_002879"
     CDS             6..398
                     /locus_tag="IVO76_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7430783.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..>491
                     /locus_tag="IVO76_002880"
     CDS             399..>491
                     /locus_tag="IVO76_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HAP7430784.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVL"
BASE COUNT          193 a           34 c           85 g          179 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt a
//
LOCUS       DACCQI010000271          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.287, whole genome shotgun sequence.
ACCESSION   DACCQI010000271 DACCQI010000000
VERSION     DACCQI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.287"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
BASE COUNT          179 a           72 c           87 g          146 t
ORIGIN      
        1 atctatattt tgatatacaa attacagctt cagatactaa gttgcataaa ccttttccgg
       61 accccataca aaaggcaatt gatgaattgg gcataaaaaa taagaaacga tttatattgg
      121 tgattcatta tatgacatgc aaagtgcgaa agcttgtggg atagcatttg gtttagctaa
      181 atggggagcc aaaaatatag aaaattttca aagtgtagat ataattattg attctcctca
      241 agaaatactt gatttgttag aaaaatagac aaagagcgtg agataaggat agggtctcac
      301 gctctttgtt tttcgccatt taaagattga aatttttgcc atcgccgatt gtaaaattaa
      361 gctagacaac taaaaaagtc atttgtgcta cactcaaaat agttccgacc aaagaattat
      421 aaggagtgag tacaaatgac ctatacccat cttacaacgg atgaacttgt aataatagag
      481 tctt
//
LOCUS       DACCQI010000272          473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.denovo.288, whole genome shotgun sequence.
ACCESSION   DACCQI010000272 DACCQI010000000
VERSION     DACCQI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.denovo.288"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..>473
                     /locus_tag="IVO76_002881"
     CDS             <1..>473
                     /locus_tag="IVO76_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7430785.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVST"
BASE COUNT          161 a           92 c           95 g          125 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct act
//
LOCUS       DACCQI010000273        20862 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.051, whole genome shotgun sequence.
ACCESSION   DACCQI010000273 DACCQI010000000
VERSION     DACCQI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.051"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            267..1304
                     /locus_tag="IVO76_002882"
     CDS             267..1304
                     /locus_tag="IVO76_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7430786.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IVO76_002883"
     CDS             complement(1393..1716)
                     /locus_tag="IVO76_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7430787.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IVO76_002884"
     CDS             complement(1703..2410)
                     /locus_tag="IVO76_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7430788.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IVO76_002885"
     CDS             2496..2837
                     /locus_tag="IVO76_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP7430789.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IVO76_002886"
     CDS             complement(3008..3856)
                     /locus_tag="IVO76_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430790.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IVO76_002887"
     CDS             4454..5317
                     /locus_tag="IVO76_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7430791.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IVO76_002888"
     CDS             5417..5899
                     /locus_tag="IVO76_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7430792.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQEVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IVO76_002889"
     CDS             complement(6162..6629)
                     /locus_tag="IVO76_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7430793.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IVO76_002890"
     CDS             6752..7603
                     /locus_tag="IVO76_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7430794.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IVO76_002891"
     CDS             complement(7854..9812)
                     /locus_tag="IVO76_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7430795.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IVO76_002892"
     CDS             complement(9809..10681)
                     /locus_tag="IVO76_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7430796.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IVO76_002893"
     CDS             complement(11023..11895)
                     /locus_tag="IVO76_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7430797.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IVO76_002894"
     CDS             12023..12592
                     /locus_tag="IVO76_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7430798.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IVO76_002895"
     CDS             12659..13396
                     /locus_tag="IVO76_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7430799.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IVO76_002896"
     CDS             13627..14895
                     /locus_tag="IVO76_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7430800.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IVO76_002897"
     CDS             15382..15846
                     /locus_tag="IVO76_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7430801.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IVO76_002898"
     CDS             15830..16459
                     /locus_tag="IVO76_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7430802.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IVO76_002899"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IVO76_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7430803.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IVO76_002900"
     CDS             18338..18784
                     /locus_tag="IVO76_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7430804.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IVO76_002901"
     CDS             complement(18983..19498)
                     /locus_tag="IVO76_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7430805.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="IVO76_002902"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="IVO76_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6631 a         3703 c         4316 g         6212 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt atggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg aggttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtgta ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DACCQI010000274        12362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.087, whole genome shotgun sequence.
ACCESSION   DACCQI010000274 DACCQI010000000
VERSION     DACCQI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.087"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..711)
                     /locus_tag="IVO76_002903"
     CDS             complement(<1..711)
                     /locus_tag="IVO76_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7430806.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKE"
     gene            complement(672..1001)
                     /locus_tag="IVO76_002904"
     CDS             complement(672..1001)
                     /locus_tag="IVO76_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7430807.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(1259..1630)
                     /locus_tag="IVO76_002905"
     CDS             complement(1259..1630)
                     /locus_tag="IVO76_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430808.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            1775..1960
                     /locus_tag="IVO76_002906"
     CDS             1775..1960
                     /locus_tag="IVO76_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7430809.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            2903..3562
                     /locus_tag="IVO76_002907"
     CDS             2903..3562
                     /locus_tag="IVO76_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7430810.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            3565..4908
                     /gene="vanS"
                     /locus_tag="IVO76_002908"
     CDS             3565..4908
                     /gene="vanS"
                     /locus_tag="IVO76_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7430811.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RSDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            5079..5888
                     /gene="vanY"
                     /locus_tag="IVO76_002909"
     CDS             5079..5888
                     /gene="vanY"
                     /locus_tag="IVO76_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP7430812.1"
                     /translation="MEKKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGW
                     RLASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIP
                     AQYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKI
                     AEYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSY
                     RFGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            5906..6733
                     /gene="vanW"
                     /locus_tag="IVO76_002910"
     CDS             5906..6733
                     /gene="vanW"
                     /locus_tag="IVO76_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7430813.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            6730..7701
                     /locus_tag="IVO76_002911"
     CDS             6730..7701
                     /locus_tag="IVO76_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7430814.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            7694..8722
                     /gene="vanB"
                     /locus_tag="IVO76_002912"
     CDS             7694..8722
                     /gene="vanB"
                     /locus_tag="IVO76_002912"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7430815.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAGPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            8728..9336
                     /locus_tag="IVO76_002913"
     CDS             8728..9336
                     /locus_tag="IVO76_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7430816.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            9924..10355
                     /locus_tag="IVO76_002914"
     CDS             9924..10355
                     /locus_tag="IVO76_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7430817.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            10362..10595
                     /locus_tag="IVO76_002915"
     CDS             10362..10595
                     /locus_tag="IVO76_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7430818.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(10517..10759)
                     /locus_tag="IVO76_002916"
     CDS             complement(10517..10759)
                     /locus_tag="IVO76_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430819.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            11016..11219
                     /locus_tag="IVO76_002917"
     CDS             11016..11219
                     /locus_tag="IVO76_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7430820.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            11303..>12362
                     /locus_tag="IVO76_002918"
     CDS             11303..>12362
                     /locus_tag="IVO76_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7430821.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTR"
BASE COUNT         3370 a         2734 c         3229 g         3029 t
ORIGIN      
        1 ctctttgatg gcttcctcgg tgtaggcttc ccccaatgtc ttgcagcggg taaagcgttc
       61 ctgtccggga gcgcggaaag aaataaactt tcccggcttg atctcatagc cctgcgcctg
      121 catgagccgc aggaaatcat caaaatcttt tgcctgcggg atagcggcgt caatggctgc
      181 tttgagcttc gctttccagc tcgtcccttt ccgctgggcg tcccactcgg catagctctt
      241 gcccttactc tggccgggct tcaccacgga caatccgttt tccttgcaca gcctgtcgct
      301 ggtgcgccgg atataggcat agctctgttt gttggaaatg tacttgcgct gatttaccat
      361 atccacggca cagaaaataa tgtgattgtg gacgtggccc ttatcaatat gggtagtcag
      421 cacataggga tatttccctt gcagcacttc ctcggcaagc tgcttcccga tctcatgggc
      481 ctgttccggc gtggcttcgc cgggggcaaa ggattgtatc aggtggtggc ccagattatt
      541 gcccttttgg tatgcctgcg ccagcagcat ttcaaattca atgtccgctg tttcataaga
      601 gcaggcaaag gaggccacca gcagcttgcc gtcagttttg tccgggttct cgatgtagtc
      661 aagggctttt ttcagcgttg acttgatagg atgtatcttt gtaactgcca tatctgcgcc
      721 agcgccccct ttatatcctc gatgtcctgt gcgtacaccg gccccatacc gttcagcctc
      781 tttgcaatct gattgacgtt gacgccgatc ttctgtatct cggcggtctg cgcccggatg
      841 tcgctggtgt ccatgttgac gatatagccg tcaatgagca tcttccgggc gtaggctgaa
      901 aagttcttcg tatgcagcag cgccattttc tgttcaatca tagcccgttc ctccggcgtc
      961 acagggacgc gcagcacgat ttttcgcttt ctgtttgcca tgcttcaccg tcctttccat
     1021 tcaggggttt ggggatttcc ccaacaagca aaacggacgc ggcccggcag ggacggggca
     1081 ggccgttttt ccgggagtgg ctacactccc tatgcttgct acgttactgt ttctccacta
     1141 acaagacgtt tccaaagccc caaactaccc gcaaaatagc cctctggaaa gaaaaaagtg
     1201 caaaaaaata gaagctacgc tgtgcagctc ccgctaaaag taaaacagag gatgccagtt
     1261 acacatcctc cgtttcattc ttcttcggct tttcaattag tatggcgctg tcaatcgcgc
     1321 cctcgatgat cggcaggtat tcttcggggc agagtttcag cttgtggctg accctttgcc
     1381 gcagttcgct ttcctcccgc atgatctccg ggttaaagta gcgttccaca ggaagcccgc
     1441 aggctttaat aagctgtatg acaaccggca gactgggaat ggccccgtcg ttttcgatgt
     1501 tggcaagata gcgccagtca atatttattt tctctgccat tgctttgcgc gaaacgcgct
     1561 ttgcttgccg ggccgctttc acatccgcgc cgaaagtttc aaagccggga caatcttcaa
     1621 tattcgccat atcgcacctc ccacttacat tgtatatttc atactctgcc cgtggaatgt
     1681 tggtatatgc acctttctta aagtttatat ttcacatttt gtttctttta ttcttcgagc
     1741 aaaagaatta taatgtaccc tgtggaggtg tactatggac tacatgacgt tgaaagaggc
     1801 aggcgagaaa tggggtgtgc ccccacgatg gataaactac tattgtgctg cgggacgcat
     1861 ccccggcgct gtaaagatgg cgacgatctg gctcattccc aaaactgcgg aaaagccgat
     1921 tgacaggcgt acaaagaaag gaaaggcggt tactgaatag tataatgaaa ccggcgcatt
     1981 ttggatatgg ttttgaagag agagaaagca tggtattttg tcacggaaaa actataagat
     2041 gatagatggc aggctgctcc agaccgataa aaagtattct catctgaaac agaaagaaaa
     2101 gatttacaca tggatgtatg aagaagcgaa actttatcat gacagaaatg gaaaatgccc
     2161 gaaacaaaga cgaggaagct gctgctgtat cagccgtata tggtagaatt gaaaaggccg
     2221 gcatctggat tctttatggt gaagtctcca gacattggat aactgctggt ctaatgcgcc
     2281 tttgagtatg gggtgattga ttttaatacc ggggaggtac ggctttttga ggaaaatatt
     2341 ctgggattta catttggcga ttattacatt tcctgtaact gcaaggacac ggatgatgaa
     2401 gatatgagga atatagactt gctgattttc tactgtcttg aacggtatta ggactgaatc
     2461 tgctgcgcgg agtgcggcaa gagcgaccgg agggagtaca cacaggccct gcctgtgtga
     2521 aaagccatac cgcaaggtgc attcgcaaaa aagaggccgc gcattttgct gaggcttggg
     2581 gtgtccccaa caagcaaaac gagaacgccg caggcgggca agtggtggat cagaatatcc
     2641 gtaaaggata ttctgatcca ttgcttgcca gtattatcgg gatgttttga caaccgctgt
     2701 aatcgaaaca tcccaaacat taagaaggtg ttaattatta ttatcttgat ttaattcgtt
     2761 taaaccaata atatagatac atacaccatg aaaagaagcg agtatcccaa tgacggatga
     2821 ttaaaatccg aaaagaaatc gctttgaaac tacagggaaa ctacagactg ttatgttatc
     2881 ttcttaaaat ggagggattt ttatgtcgat acgaattcta cttgtcgagg atgatgatca
     2941 tatctgtaat acagtaaggg cgtttctggc tgaggcagga tatcaggtgg atgcctgcac
     3001 agatggaaat gaggcataca ccaagtttta cgaaaacact tatcaactgg ttattcttga
     3061 tattatgctg cccggtatga acgggcatga acttttgcgt gaatttcgtg cgaaaaatga
     3121 tactcccatt ctgatgatga cagccctgtc ggatgacgaa aaccaaatcc gggcgtttga
     3181 tgcagaggca gacgactatg taacaaagcc attcaagatg cagattttac taaagcgggt
     3241 ggaagccctg ctgcggcgca gcggtgcgct ggcaaaggaa atccgtgtcg gcaggctgac
     3301 acttctgccg gaggatttca cggtactttg tgacggtaca gagctgcccc tgacacgaaa
     3361 agaatttgaa atccttttgc tgctggtgca gaacaaaggc agaaccttaa cgcatgaaat
     3421 cattttgtca cgtatatggg gatatgactt tgagggtgat ggcagcacag tccacactca
     3481 tatcaaaaat ttgcgggcca agctgccgga aaatatcatc aaaaccattc gcggtgtagg
     3541 ataccgattg gaggaatcat aataatggaa agaaaaggga ttttcattaa ggttttttcc
     3601 tatacgatca ttgtcctgct actgcttgtc ggtgtaacag caacactgtt tgcacagcaa
     3661 tttgtgtctt atttcagagt gatggaatta cagcaaacag taaaatccta tcagccattg
     3721 gtggaactga ttcagaatag cgataggctt gatattcaag aggtggcagg gctgtttcac
     3781 tacaataacc aatcctttga gttttatatt gaagataaag agggaagcgt actctatgcc
     3841 acaccaaatg ccaatacatc aaatagtttt agatccgact ttctttatgt ggtacataga
     3901 gatgataata tttcgattgt tgctcaaagc aaggcaggtg tgggattgct ttatcaaggg
     3961 ctgacaattc ggggaattgt tatgattgcg ataatggttg tattcagcct tttatgcgcg
     4021 tatatctttg cgcggcaaat gacaacgccg atcaaagcct tagcggacag tgcgaataaa
     4081 atggcaaacc tgaaagatgt accgccgccg ctggagcgaa aggatgagct tggcgcactg
     4141 gctcatgata tgcattccat gtatgtcagg ctgaaagaaa ccatcgcaag gctggaggat
     4201 gaaatcgcaa gggaacatga gttggaggaa acacagcgat atttctttgc ggcagcttct
     4261 catgagctaa aaacgcccat cgcggctaca agcgttctgt tggagggaat gcttgaaaat
     4321 atcggtgact acaaagatca ttctaagtat ctgcgcgaat gcatcaaaat gatggatagg
     4381 cagggcaaaa tcatttccga aatactggag cttgtcagcc tgaatgatgg gagaatcgta
     4441 cccatagctg aaccgttgga catagggcgc acggttgccg agttgctgcc cgattttcaa
     4501 accttggcag aggcaaacaa ccagcggttc gtcacagata ttccagccgg gcaaattgtc
     4561 ctgtccgatc cgaggctgct ccaaaaggca ctatccaatg tcatattgaa tgcagttcag
     4621 aacacgccgc agggaggcga ggtacggata tggagtgagc ctggtgctga aaaatgccgc
     4681 ctttttgttt tgaacatggg cgttcacatt gatgatactg cgcttccaag gctgttcacc
     4741 ccattctatc gcattgatca ggcgcgaagc agaaaaagtg ggcgaagcgg tttaggactt
     4801 gccatcgtac aaaaaacgct ggatgccatg agcctccagt atgcgctgga aaacacctcg
     4861 gatggcgttt tgttctggct ggatttaccg ctcacatcaa cattgtaaat atttaaaact
     4921 taaatgattt tgaccgacag gtataaccct gccggtcttt ttttgctgct acaggaaaac
     4981 tacagactgg ctacagggaa agtacagatg cgcttgccat aataacaatc gtaccagcca
     5041 caaatcgtag ttttattgca aaggaggcat tcgatcaaat ggaaaaaaaa agcaactatc
     5101 attccaatgc ggatcatcac aaacggcata tgaaacaatc cgtggaaaag cgggcttttc
     5161 tatgtgcgtt cattatttcg ttcacagtct gcacgctgtt tttggggtgg agactggctt
     5221 ccgtattgga ggcaacacag ataccgccca tccctgcaac tcatacaggc agcagcactg
     5281 acgtagtgga gaatttggag gaaaacgctc ttgccaccgc caaagaacag ggagatgaac
     5341 aggaatggag cctgatttta gtgaacaggc agaaccccat ccccgcacag tacgatgtgg
     5401 agcttgagca actatcaaat ggtgagcgga tagatattcg gatttctccc tatcttcaag
     5461 atttgtttga tgccgcaaga actgatggag tttacccgat tgtcgcatcc ggataccgaa
     5521 caacagaaaa acagcaagaa attatggatg aaaaaattgc cgaatataag gcgaaaggct
     5581 acacctctgc acaggctaaa gcggaagcag aaacttgggt ggccgtgccg ggaacgagcg
     5641 agcatcagct tggtcttgct gtggatatca atgcggacgg aattcattca acaggcaacg
     5701 aggtttatag atggctggat gaaaacagct atcgttttgg ttttattcgt cgctacccac
     5761 cagacaagac agagataacc ggtgtgagca acgagccgtg gcattaccga tatgttggca
     5821 tcgaagctgc cacagagatg tacaaccaag gggtttgcct tgaggaatat ttaaaaccag
     5881 aaaaatgaga aaaggatgtt atgttatgga cagaaaaaga ttgacacagc gtttcccgtt
     5941 cctgcttcca atgagacggg cgcagagaaa aatgtgcttt tatgcgggaa tgagatttga
     6001 cggctgtcgc tatgcgcaga cgatagggga aaaatcgctt tcccatttgc tctttgaaac
     6061 ggattgcgca ttatacaacc acaataccgg atttgacatg atataccagg aaaacaaggt
     6121 gttcaactta aagctggcag caaagacctt aaacggctta ttgataagac cgggggaaac
     6181 cttttctttc tggtggctgg tacgccatgc ggacaaagat accccctata aagacggcct
     6241 tacggtgacc aatggtaaac tcaccaccat gtcgggcggc ggtatgtgcc agatgagcaa
     6301 tttactattt tggatgttcc tgcatacgcc attgacaatt atccagcgca gaggtcacga
     6361 agtaaaggag tttccagagc caaacagtga cgagattaaa ggggtggatg caaccatttc
     6421 agagggctgg attgatttaa aagtgcgaaa cgataccgac tgcacctacc aaatatgggt
     6481 gaccttagat gatgagaaaa tcatcggtca ggtgtccgcc gacaaagagc cccaagcatt
     6541 atacaaaatt acaaatggca gtatccagta tgtccgtgaa agtggcggga tttatgaata
     6601 tgcccaggtt aaacggatgc aagttgcctt aggtaccggg gaaataatag attgcaagct
     6661 gctttataca aacaaatgca aaatctgtta tcccctcccg gaaagtgtgg atattcagga
     6721 ggagaaccaa tgagaaaaag tgtgggcatt actgtttttg gatgcgaaca ggatgaggca
     6781 aatgctttcc gcgctttatc gccggatttt catattatcc ctacgctgat tagcgatgcg
     6841 atatcggcag acaacgcaaa attggccgct ggcaatcaat gcgttagcgt aggccataag
     6901 tccgaggttt ccgaggcgac aattcttgcg ctgagaaagg tcggggtaaa atacatttct
     6961 acccgcagca tcggctgcga tcacattgat acgactgccg ccgagagaat gggaatctcg
     7021 gttggcacgg ttgcgtattc gccggacagc gttgcggatt atgctttgat gctgatgctg
     7081 atggccatac ggggtgcaaa acccaccatg cacgccgtgg cgcaacaaga tttcagattg
     7141 gatcgtatcc gggggaaaga actgggggat atgactgtgg gagttattgg aaccggccat
     7201 atcgggcaag cggtcgtcaa aaggctgcgg ggatttggat gccatgtgct ggcctatgat
     7261 aacagccgaa aaatggatgc agattatgtc cagcttgatg agcttctaaa aaacagcgat
     7321 attgttacgc tccatgtgcc gctttgtgcg gatacccgcc atctgatcgg tcagaagcaa
     7381 attggagaga tgaagcaagg cgcattttta atcaacaccg ggcgcggagc gcttgtcgat
     7441 accggggcgc tggtggaggc gttggaaagc ggaaagctgg gtggtgcggc actggatgtg
     7501 ttggagggcg aggatcagtt tgtttatacc gattgctcgc agaaagtgct tgaccatccc
     7561 tttttgtcgc agcttttaag gatgccgaat gtgatcatca caccccatac ggcgtactac
     7621 actgagcgtg tgctgcagga tactacagaa aaaacaatca ggaattgtct caattttgaa
     7681 aggagtttac agcatgaata gaataaaagt cgcaatcatc ttcggcggtt gctcggagga
     7741 acatgatgtg tcggtaaaat ccgcaataga aattgctgcg aacattgata cggaaaaatt
     7801 cgatccgcac tacatcggaa ttacaaaaaa cggtgtatgg aagctatgca agaagccatg
     7861 tacggaatgg gaagccgaca gtctccccgc catactctcc ccggatagga aaacgcatgg
     7921 gctgcttgtc atgaaagaaa gcgaatacga aacacggcgt attgatgtgg ctttcccggt
     7981 tttgcatggc aaatgcgggg aggatggtgc gatacagggg ctgtttgtat tgtctggtat
     8041 cccctatgtg ggctgtgata ttcaaagctc cgcagcttgc atggacaaat cactggccta
     8101 cattcttaca aaaaatgcgg gcatcgccgg tcccgaattt caaatgattg ataaaggtga
     8161 caagccggag gcgggtgcgc ttacctaccc tgtctttgtg aagccggcac ggtcaggttc
     8221 gtcctttggc gtaaccaaag taaacggtac ggaagaactt aacgctgcga tagaagcggc
     8281 aggacaatat gatggaaaaa tcttaattga gcaagcgatt tcgggctgtg aggtcgggtg
     8341 tgcggtcatg gggaacgagg atgatttgat tgtcggcgaa gtggatcaaa tccggctgag
     8401 ccacggtatc ttccgcatcc atcaggaaaa cgagccggaa aaaggctcag aaaatgcgat
     8461 gattacagtt cccgcagaca ttccggtcga ggaacgaaat cgggtgcagg aaacggcaaa
     8521 gaaagtatat cgggtgcttg gatgcagagg gcttgcccgt gttgatcttt ttttgcagga
     8581 ggatggcggc atcgttctaa atgaggtcaa taccatgccc ggttttacat cgtacagccg
     8641 ctacccacgt atggtggccg ccgcaggaat cacgcttcct gcactgattg acagcctgat
     8701 tacattggcg ttaaagaggt gataagtatg gaaaatggtt ttttgttttt agatgaaatg
     8761 ttgcatggcg tccgttggga tgccaagtac gccacatggg ataacttcac aggaaaaccg
     8821 gtggatgggt atgaggtgaa tcgcatcatc ggcacaaagg ccgtggcgtt tgctctgcgc
     8881 gaggcacaaa tccatgcggc tgcgcttggc tatggcttgc ttttatggga tggatatcgg
     8941 ccaagaactg cggtggactg cttcctgcgt tgggcagcgc aaccggagga caagctcaca
     9001 aaagaaaaat tttaccccaa tatagagcga gccgagttga ttacaaaggg ttatgtggcc
     9061 tcacaatcca gccatagccg tggaagcgca attgatctta cgctctacca cctggataca
     9121 ggggaacttg tttcaatggg aagtaacttc gattttatgg acgaacggtc gcatcataca
     9181 gcaaaaggga taggggatgc agaggcacaa aatcgaagat gcttgcgtaa aatcatggaa
     9241 agcagcggat ttcagtctta tcgctttgaa tggtggcact ataagttgat tgatgagcca
     9301 taccccgata cctattttaa ttttgccgtt tcataatgaa agtattggtt ttgtatcaga
     9361 ttttggattt gataaaatct atgctggtca tggacaatct tttactggaa agtggttaaa
     9421 gcatcttttg taatacattg caaattatag tattcaatct gcccagcggc agaaaagaaa
     9481 aaaaccgctg ttgggcagac ctttcccacg cccattttgt attttgctgg cggcggtttt
     9541 gagaaatcaa ggccgccgtt cttttctggc agcaaaaggt gtggcggccc ataggaggat
     9601 gccgctatgc tgttttgcta ttgccatgct ctaatccact tccaccactc aaaaaaagcc
     9661 ttgcccctcc acagggctat tccggttatg agtatgtact ataccatgta aatcagcagc
     9721 atagcaaagc attttctcgc ccggtaggtt tgcgacctgc cgggcgattt ttgtcgtctt
     9781 ttgcggcccg gctgtcccca aaaaaatttt ttgagaaatt ttcaaaaagg aggtatttag
     9841 cgggtagctg gcggccttgt caaagtcttg ttagcggaaa gggagagaac cacctaagtc
     9901 ccccacagaa agggggtgag aagatggatg ctatcccccg cgactatgaa gcgcggtgca
     9961 tgttcgatgc tttttgcaag acggtactgc gtaacgaggc caaaagctac ttggctgaaa
    10021 tgaagcgccg ccgtgaccgt gaagtgtcac taagctcttt gtcacaggca gatttggaca
    10081 agctctgtac agtagatcac taccccagcg acagctttac gttttcgtcg catggctatg
    10141 acctacatat caacaatgag cttgttgccg aagcctttgc cgcgctgccc agcatggagc
    10201 agagtatttt aatcctgcac tgtgttctgg atatggcaga tggtgagatc ggcggccttg
    10261 tgggaatgtc ccggagtgcc gttcagcggc acaggacaaa taccttatcg gagctgcgga
    10321 aacagctaaa ggcgttgatg ccgaagggag gttagacgcg gatgaagaac ccgaactaca
    10381 agggcattga gcttttgccc ctttcggtga tcgaggccgc ccgcgctggg gatgccggag
    10441 ccgtagagcg cgttttgcag tattacagcg gttacatgaa caagctgtgt acccggacgc
    10501 tctatgacga aaacggctat ccgcatgtgt gtctggacga ggccatgaag cgccgtctgg
    10561 aaatcaagct catccacgct atcgtcacga actgacgaac cggccccggc agcatgaaac
    10621 ggcttaccct ttccccgttt cggtgttttc ccggtggcct gatcgcacct tgataaatat
    10681 gcccgcaaga taacggcggc gcatcatagg gcatacggac acattctgtc tgtgccgagc
    10741 cggacggctg gtgcgccatg atacttttcc ctacgggaaa agcggagcga ggaacaccac
    10801 gccgcaatgt gagcgtggcg gcttgcgggc gacgacgtac aggcaggact atgatactcc
    10861 cttatagcca cagtccgagc gttaaaagcg tcgcaggcaa tgggtagggc cgcgtgagaa
    10921 ccacgcgggg gtgaaagtcc cgtggagctg tgccagcagc cgtccgtcta tgcccctaaa
    10981 tcacacaaac ctttgaattt gtgaaagggg gctgcattat gaaccatgat gtgcctattt
    11041 gggaaaaata cacgctgacg attgaggaag cgtctaaata tttccgtatc ggagaaaaca
    11101 aattgcgtaa gctggccgag gaaaatccgg cctctggctg ggtcattatg aatggcaacc
    11161 gcatccagat caaacgcaag cagtttgaga aaataattga cactttggac acaatctaat
    11221 agtgaaaaag ggccgcagat atgttataat aatttataag catatatgcg gctctttccg
    11281 tcatggaaag gagcatgacg agatgtcaga aaaaagacga gacagcaaaa atcgcgtttt
    11341 gcggtctgga gagagccaga gaaaagacgg acgatatgct tacaaatatg ttgatacctt
    11401 tggaaaaccg cagtttgttt actcatggaa gttagtgcct acggataaga ccccggcagg
    11461 taaacgggag gatatttcac tccgggaaaa ggagaaagaa atccagaaag accttgacga
    11521 cgggatagac accatcggca agaaaatgac cgtctgccag ctctacgcca agcagatcag
    11581 gcaccggggc aatgtgaagc acaacaccaa actgggccgg gaacggctga tgcgtattct
    11641 ggaacaagat cggctgggga gctgcccgat tgacagtgtg aagctgtccg acgcaaagga
    11701 atgggcgctt cggatgaaag aaaagggcct ttcttataag accatcaata acgacaagcg
    11761 ttccctgaaa gcggcttttt acaccgccat acaggacgat tgcatccgaa aaaacccgtt
    11821 tgacttccag cttagtgacg ttctggacga cgatacggaa ccaaaggtgc ctcttacacc
    11881 agcacaggaa gaaagttttc tgtcctttat tcaaggtgat aaggtttacc aaaagcatta
    11941 tgacgcaatc gttattctgc tggggacagg gcttcgcatt tcggaactgt gcggactgac
    12001 ggacaaagat ttggattttg aaaaccgggt aatcatcgtg agccaccagc ttttgagaaa
    12061 taccggcgtc ggttactata ttgacgaacc caagacccaa agcggcgtca gaaaaatccc
    12121 catgaatgag gaagtgtatc aggcgttcca gcgagtgata aaaaaccgca aaggtgcaaa
    12181 gccgttcatt attgacgggt atgccaattt cctgtttctc aaacagaacg gctatcccat
    12241 gacggcggta gattatggcg ggatgtttgg caggcttgtg aagaagtaca acaagagcca
    12301 tgaagaagcg ttgccgaaaa cgacgacgcc ccatgctatg cgtcatacat tctgcacccg
    12361 gt
//
LOCUS       DACCQI010000275        12362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.088, whole genome shotgun sequence.
ACCESSION   DACCQI010000275 DACCQI010000000
VERSION     DACCQI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.088"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..711)
                     /locus_tag="IVO76_002919"
     CDS             complement(<1..711)
                     /locus_tag="IVO76_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7430822.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKE"
     gene            complement(672..1001)
                     /locus_tag="IVO76_002920"
     CDS             complement(672..1001)
                     /locus_tag="IVO76_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7430823.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(1259..1630)
                     /locus_tag="IVO76_002921"
     CDS             complement(1259..1630)
                     /locus_tag="IVO76_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430824.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            1775..1960
                     /locus_tag="IVO76_002922"
     CDS             1775..1960
                     /locus_tag="IVO76_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7430825.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            2903..3562
                     /locus_tag="IVO76_002923"
     CDS             2903..3562
                     /locus_tag="IVO76_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7430826.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            3565..4908
                     /gene="vanS"
                     /locus_tag="IVO76_002924"
     CDS             3565..4908
                     /gene="vanS"
                     /locus_tag="IVO76_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7430827.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RSDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            5079..5888
                     /gene="vanY"
                     /locus_tag="IVO76_002925"
     CDS             5079..5888
                     /gene="vanY"
                     /locus_tag="IVO76_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP7430828.1"
                     /translation="MEKKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGW
                     RLASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIP
                     AQYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKI
                     AEYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSY
                     RFGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            5906..6733
                     /gene="vanW"
                     /locus_tag="IVO76_002926"
     CDS             5906..6733
                     /gene="vanW"
                     /locus_tag="IVO76_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
                     /protein_id="HAP7430829.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWWLVRHA
                     DKDTPYKDGLTVTNGKLTTMSGGGMCQMSNLLFWMFLHTPLTIIQRRGHEVKEFPEPN
                     SDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKIIGQVSADKEPQALYKITNG
                     SIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKCKICYPLPESVDIQEENQ"
     gene            6730..7701
                     /locus_tag="IVO76_002927"
     CDS             6730..7701
                     /locus_tag="IVO76_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7430830.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            7694..8722
                     /gene="vanB"
                     /locus_tag="IVO76_002928"
     CDS             7694..8722
                     /gene="vanB"
                     /locus_tag="IVO76_002928"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7430831.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            8728..9336
                     /locus_tag="IVO76_002929"
     CDS             8728..9336
                     /locus_tag="IVO76_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7430832.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDKLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            9924..10355
                     /locus_tag="IVO76_002930"
     CDS             9924..10355
                     /locus_tag="IVO76_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7430833.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            10362..10595
                     /locus_tag="IVO76_002931"
     CDS             10362..10595
                     /locus_tag="IVO76_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7430834.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(10517..10759)
                     /locus_tag="IVO76_002932"
     CDS             complement(10517..10759)
                     /locus_tag="IVO76_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430835.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            11016..11219
                     /locus_tag="IVO76_002933"
     CDS             11016..11219
                     /locus_tag="IVO76_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7430836.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            11303..>12362
                     /locus_tag="IVO76_002934"
     CDS             11303..>12362
                     /locus_tag="IVO76_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7430837.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTR"
BASE COUNT         3370 a         2734 c         3228 g         3030 t
ORIGIN      
        1 ctctttgatg gcttcctcgg tgtaggcttc ccccaatgtc ttgcagcggg taaagcgttc
       61 ctgtccggga gcgcggaaag aaataaactt tcccggcttg atctcatagc cctgcgcctg
      121 catgagccgc aggaaatcat caaaatcttt tgcctgcggg atagcggcgt caatggctgc
      181 tttgagcttc gctttccagc tcgtcccttt ccgctgggcg tcccactcgg catagctctt
      241 gcccttactc tggccgggct tcaccacgga caatccgttt tccttgcaca gcctgtcgct
      301 ggtgcgccgg atataggcat agctctgttt gttggaaatg tacttgcgct gatttaccat
      361 atccacggca cagaaaataa tgtgattgtg gacgtggccc ttatcaatat gggtagtcag
      421 cacataggga tatttccctt gcagcacttc ctcggcaagc tgcttcccga tctcatgggc
      481 ctgttccggc gtggcttcgc cgggggcaaa ggattgtatc aggtggtggc ccagattatt
      541 gcccttttgg tatgcctgcg ccagcagcat ttcaaattca atgtccgctg tttcataaga
      601 gcaggcaaag gaggccacca gcagcttgcc gtcagttttg tccgggttct cgatgtagtc
      661 aagggctttt ttcagcgttg acttgatagg atgtatcttt gtaactgcca tatctgcgcc
      721 agcgccccct ttatatcctc gatgtcctgt gcgtacaccg gccccatacc gttcagcctc
      781 tttgcaatct gattgacgtt gacgccgatc ttctgtatct cggcggtctg cgcccggatg
      841 tcgctggtgt ccatgttgac gatatagccg tcaatgagca tcttccgggc gtaggctgaa
      901 aagttcttcg tatgcagcag cgccattttc tgttcaatca tagcccgttc ctccggcgtc
      961 acagggacgc gcagcacgat ttttcgcttt ctgtttgcca tgcttcaccg tcctttccat
     1021 tcaggggttt ggggatttcc ccaacaagca aaacggacgc ggcccggcag ggacggggca
     1081 ggccgttttt ccgggagtgg ctacactccc tatgcttgct acgttactgt ttctccacta
     1141 acaagacgtt tccaaagccc caaactaccc gcaaaatagc cctctggaaa gaaaaaagtg
     1201 caaaaaaata gaagctacgc tgtgcagctc ccgctaaaag taaaacagag gatgccagtt
     1261 acacatcctc cgtttcattc ttcttcggct tttcaattag tatggcgctg tcaatcgcgc
     1321 cctcgatgat cggcaggtat tcttcggggc agagtttcag cttgtggctg accctttgcc
     1381 gcagttcgct ttcctcccgc atgatctccg ggttaaagta gcgttccaca ggaagcccgc
     1441 aggctttaat aagctgtatg acaaccggca gactgggaat ggccccgtcg ttttcgatgt
     1501 tggcaagata gcgccagtca atatttattt tctctgccat tgctttgcgc gaaacgcgct
     1561 ttgcttgccg ggccgctttc acatccgcgc cgaaagtttc aaagccggga caatcttcaa
     1621 tattcgccat atcgcacctc ccacttacat tgtatatttc atactctgcc cgtggaatgt
     1681 tggtatatgc acctttctta aagtttatat ttcacatttt gtttctttta ttcttcgagc
     1741 aaaagaatta taatgtaccc tgtggaggtg tactatggac tacatgacgt tgaaagaggc
     1801 aggcgagaaa tggggtgtgc ccccacgatg gataaactac tattgtgctg cgggacgcat
     1861 ccccggcgct gtaaagatgg cgacgatctg gctcattccc aaaactgcgg aaaagccgat
     1921 tgacaggcgt acaaagaaag gaaaggcggt tactgaatag tataatgaaa ccggcgcatt
     1981 ttggatatgg ttttgaagag agagaaagca tggtattttg tcacggaaaa actataagat
     2041 gatagatggc aggctgctcc agaccgataa aaagtattct catctgaaac agaaagaaaa
     2101 gatttacaca tggatgtatg aagaagcgaa actttatcat gacagaaatg gaaaatgccc
     2161 gaaacaaaga cgaggaagct gctgctgtat cagccgtata tggtagaatt gaaaaggccg
     2221 gcatctggat tctttatggt gaagtctcca gacattggat aactgctggt ctaatgcgcc
     2281 tttgagtatg gggtgattga ttttaatacc ggggaggtac ggctttttga ggaaaatatt
     2341 ctgggattta catttggcga ttattacatt tcctgtaact gcaaggacac ggatgatgaa
     2401 gatatgagga atatagactt gctgattttc tactgtcttg aacggtatta ggactgaatc
     2461 tgctgcgcgg agtgcggcaa gagcgaccgg agggagtaca cacaggccct gcctgtgtga
     2521 aaagccatac cgcaaggtgc attcgcaaaa aagaggccgc gcattttgct gaggcttggg
     2581 gtgtccccaa caagcaaaac gagaacgccg caggcgggca agtggtggat cagaatatcc
     2641 gtaaaggata ttctgatcca ttgcttgcca gtattatcgg gatgttttga caaccgctgt
     2701 aatcgaaaca tcccaaacat taagaaggtg ttaattatta ttatcttgat ttaattcgtt
     2761 taaaccaata atatagatac atacaccatg aaaagaagcg agtatcccaa tgacggatga
     2821 ttaaaatccg aaaagaaatc gctttgaaac tacagggaaa ctacagactg ttatgttatc
     2881 ttcttaaaat ggagggattt ttatgtcgat acgaattcta cttgtcgagg atgatgatca
     2941 tatctgtaat acagtaaggg cgtttctggc tgaggcagga tatcaggtgg atgcctgcac
     3001 agatggaaat gaggcataca ccaagtttta cgaaaacact tatcaactgg ttattcttga
     3061 tattatgctg cccggtatga acgggcatga acttttgcgt gaatttcgtg cgaaaaatga
     3121 tactcccatt ctgatgatga cagccctgtc ggatgacgaa aaccaaatcc gggcgtttga
     3181 tgcagaggca gacgactatg taacaaagcc attcaagatg cagattttac taaagcgggt
     3241 ggaagccctg ctgcggcgca gcggtgcgct ggcaaaggaa atccgtgtcg gcaggctgac
     3301 acttctgccg gaggatttca cggtactttg tgacggtaca gagctgcccc tgacacgaaa
     3361 agaatttgaa atccttttgc tgctggtgca gaacaaaggc agaaccttaa cgcatgaaat
     3421 cattttgtca cgtatatggg gatatgactt tgagggtgat ggcagcacag tccacactca
     3481 tatcaaaaat ttgcgggcca agctgccgga aaatatcatc aaaaccattc gcggtgtagg
     3541 ataccgattg gaggaatcat aataatggaa agaaaaggga ttttcattaa ggttttttcc
     3601 tatacgatca ttgtcctgct actgcttgtc ggtgtaacag caacactgtt tgcacagcaa
     3661 tttgtgtctt atttcagagt gatggaatta cagcaaacag taaaatccta tcagccattg
     3721 gtggaactga ttcagaatag cgataggctt gatattcaag aggtggcagg gctgtttcac
     3781 tacaataacc aatcctttga gttttatatt gaagataaag agggaagcgt actctatgcc
     3841 acaccaaatg ccaatacatc aaatagtttt agatccgact ttctttatgt ggtacataga
     3901 gatgataata tttcgattgt tgctcaaagc aaggcaggtg tgggattgct ttatcaaggg
     3961 ctgacaattc ggggaattgt tatgattgcg ataatggttg tattcagcct tttatgcgcg
     4021 tatatctttg cgcggcaaat gacaacgccg atcaaagcct tagcggacag tgcgaataaa
     4081 atggcaaacc tgaaagatgt accgccgccg ctggagcgaa aggatgagct tggcgcactg
     4141 gctcatgata tgcattccat gtatgtcagg ctgaaagaaa ccatcgcaag gctggaggat
     4201 gaaatcgcaa gggaacatga gttggaggaa acacagcgat atttctttgc ggcagcttct
     4261 catgagctaa aaacgcccat cgcggctaca agcgttctgt tggagggaat gcttgaaaat
     4321 atcggtgact acaaagatca ttctaagtat ctgcgcgaat gcatcaaaat gatggatagg
     4381 cagggcaaaa tcatttccga aatactggag cttgtcagcc tgaatgatgg gagaatcgta
     4441 cccatagctg aaccgttgga catagggcgc acggttgccg agttgctgcc cgattttcaa
     4501 accttggcag aggcaaacaa ccagcggttc gtcacagata ttccagccgg gcaaattgtc
     4561 ctgtccgatc cgaggctgct ccaaaaggca ctatccaatg tcatattgaa tgcagttcag
     4621 aacacgccgc agggaggcga ggtacggata tggagtgagc ctggtgctga aaaatgccgc
     4681 ctttttgttt tgaacatggg cgttcacatt gatgatactg cgcttccaag gctgttcacc
     4741 ccattctatc gcattgatca ggcgcgaagc agaaaaagtg ggcgaagcgg tttaggactt
     4801 gccatcgtac aaaaaacgct ggatgccatg agcctccagt atgcgctgga aaacacctcg
     4861 gatggcgttt tgttctggct ggatttaccg ctcacatcaa cattgtaaat atttaaaact
     4921 taaatgattt tgaccgacag gtataaccct gccggtcttt ttttgctgct acaggaaaac
     4981 tacagactgg ctacagggaa agtacagatg cgcttgccat aataacaatc gtaccagcca
     5041 caaatcgtag ttttattgca aaggaggcat tcgatcaaat ggaaaaaaaa agcaactatc
     5101 attccaatgc ggatcatcac aaacggcata tgaaacaatc cgtggaaaag cgggcttttc
     5161 tatgtgcgtt cattatttcg ttcacagtct gcacgctgtt tttggggtgg agactggctt
     5221 ccgtattgga ggcaacacag ataccgccca tccctgcaac tcatacaggc agcagcactg
     5281 acgtagtgga gaatttggag gaaaacgctc ttgccaccgc caaagaacag ggagatgaac
     5341 aggaatggag cctgatttta gtgaacaggc agaaccccat ccccgcacag tacgatgtgg
     5401 agcttgagca actatcaaat ggtgagcgga tagatattcg gatttctccc tatcttcaag
     5461 atttgtttga tgccgcaaga actgatggag tttacccgat tgtcgcatcc ggataccgaa
     5521 caacagaaaa acagcaagaa attatggatg aaaaaattgc cgaatataag gcgaaaggct
     5581 acacctctgc acaggctaaa gcggaagcag aaacttgggt ggccgtgccg ggaacgagcg
     5641 agcatcagct tggtcttgct gtggatatca atgcggacgg aattcattca acaggcaacg
     5701 aggtttatag atggctggat gaaaacagct atcgttttgg ttttattcgt cgctacccac
     5761 cagacaagac agagataacc ggtgtgagca acgagccgtg gcattaccga tatgttggca
     5821 tcgaagctgc cacagagatg tacaaccaag gggtttgcct tgaggaatat ttaaaaccag
     5881 aaaaatgaga aaaggatgtt atgttatgga cagaaaaaga ttgacacagc gtttcccgtt
     5941 cctgcttcca atgagacggg cgcagagaaa aatgtgcttt tatgcgggaa tgagatttga
     6001 cggctgtcgc tatgcgcaga cgatagggga aaaatcgctt tcccatttgc tctttgaaac
     6061 ggattgcgca ttatacaacc acaataccgg atttgacatg atataccagg aaaacaaggt
     6121 gttcaactta aagctggcag caaagacctt aaacggctta ttgataagac cgggggaaac
     6181 cttttctttc tggtggctgg tacgccatgc ggacaaagat accccctata aagacggcct
     6241 tacggtgacc aatggtaaac tcaccaccat gtcgggcggc ggtatgtgcc agatgagcaa
     6301 tttactattt tggatgttcc tgcatacgcc attgacaatt atccagcgca gaggtcacga
     6361 agtaaaggag tttccagagc caaacagtga cgagattaaa ggggtggatg caaccatttc
     6421 agagggctgg attgatttaa aagtgcgaaa cgataccgac tgcacctacc aaatatgggt
     6481 gaccttagat gatgagaaaa tcatcggtca ggtgtccgcc gacaaagagc cccaagcatt
     6541 atacaaaatt acaaatggca gtatccagta tgtccgtgaa agtggcggga tttatgaata
     6601 tgcccaggtt aaacggatgc aagttgcctt aggtaccggg gaaataatag attgcaagct
     6661 gctttataca aacaaatgca aaatctgtta tcccctcccg gaaagtgtgg atattcagga
     6721 ggagaaccaa tgagaaaaag tgtgggcatt actgtttttg gatgcgaaca ggatgaggca
     6781 aatgctttcc gcgctttatc gccggatttt catattatcc ctacgctgat tagcgatgcg
     6841 atatcggcag acaacgcaaa attggccgct ggcaatcaat gcgttagcgt aggccataag
     6901 tccgaggttt ccgaggcgac aattcttgcg ctgagaaagg tcggggtaaa atacatttct
     6961 acccgcagca tcggctgcga tcacattgat acgactgccg ccgagagaat gggaatctcg
     7021 gttggcacgg ttgcgtattc gccggacagc gttgcggatt atgctttgat gctgatgctg
     7081 atggccatac ggggtgcaaa acccaccatg cacgccgtgg cgcaacaaga tttcagattg
     7141 gatcgtatcc gggggaaaga actgggggat atgactgtgg gagttattgg aaccggccat
     7201 atcgggcaag cggtcgtcaa aaggctgcgg ggatttggat gccatgtgct ggcctatgat
     7261 aacagccgaa aaatggatgc agattatgtc cagcttgatg agcttctaaa aaacagcgat
     7321 attgttacgc tccatgtgcc gctttgtgcg gatacccgcc atctgatcgg tcagaagcaa
     7381 attggagaga tgaagcaagg cgcattttta atcaacaccg ggcgcggagc gcttgtcgat
     7441 accggggcgc tggtggaggc gttggaaagc ggaaagctgg gtggtgcggc actggatgtg
     7501 ttggagggcg aggatcagtt tgtttatacc gattgctcgc agaaagtgct tgaccatccc
     7561 tttttgtcgc agcttttaag gatgccgaat gtgatcatca caccccatac ggcgtactac
     7621 actgagcgtg tgctgcagga tactacagaa aaaacaatca ggaattgtct caattttgaa
     7681 aggagtttac agcatgaata gaataaaagt cgcaatcatc ttcggcggtt gctcggagga
     7741 acatgatgtg tcggtaaaat ccgcaataga aattgctgcg aacattgata cggaaaaatt
     7801 cgatccgcac tacatcggaa ttacaaaaaa cggtgtatgg aagctatgca agaagccatg
     7861 tacggaatgg gaagccgaca gtctccccgc catactctcc ccggatagga aaacgcatgg
     7921 gctgcttgtc atgaaagaaa gcgaatacga aacacggcgt attgatgtgg ctttcccggt
     7981 tttgcatggc aaatgcgggg aggatggtgc gatacagggg ctgtttgtat tgtctggtat
     8041 cccctatgtg ggctgtgata ttcaaagctc cgcagcttgc atggacaaat cactggccta
     8101 cattcttaca aaaaatgcgg gcatcgccgt tcccgaattt caaatgattg ataaaggtga
     8161 caagccggag gcgggtgcgc ttacctaccc tgtctttgtg aagccggcac ggtcaggttc
     8221 gtcctttggc gtaaccaaag taaacggtac ggaagaactt aacgctgcga tagaagcggc
     8281 aggacaatat gatggaaaaa tcttaattga gcaagcgatt tcgggctgtg aggtcgggtg
     8341 tgcggtcatg gggaacgagg atgatttgat tgtcggcgaa gtggatcaaa tccggctgag
     8401 ccacggtatc ttccgcatcc atcaggaaaa cgagccggaa aaaggctcag aaaatgcgat
     8461 gattacagtt cccgcagaca ttccggtcga ggaacgaaat cgggtgcagg aaacggcaaa
     8521 gaaagtatat cgggtgcttg gatgcagagg gcttgcccgt gttgatcttt ttttgcagga
     8581 ggatggcggc atcgttctaa atgaggtcaa taccatgccc ggttttacat cgtacagccg
     8641 ctacccacgt atggtggccg ccgcaggaat cacgcttcct gcactgattg acagcctgat
     8701 tacattggcg ttaaagaggt gataagtatg gaaaatggtt ttttgttttt agatgaaatg
     8761 ttgcatggcg tccgttggga tgccaagtac gccacatggg ataacttcac aggaaaaccg
     8821 gtggatgggt atgaggtgaa tcgcatcatc ggcacaaagg ccgtggcgtt tgctctgcgc
     8881 gaggcacaaa tccatgcggc tgcgcttggc tatggcttgc ttttatggga tggatatcgg
     8941 ccaagaactg cggtggactg cttcctgcgt tgggcagcgc aaccggagga caagctcaca
     9001 aaagaaaaat tttaccccaa tatagagcga gccgagttga ttacaaaggg ttatgtggcc
     9061 tcacaatcca gccatagccg tggaagcgca attgatctta cgctctacca cctggataca
     9121 ggggaacttg tttcaatggg aagtaacttc gattttatgg acgaacggtc gcatcataca
     9181 gcaaaaggga taggggatgc agaggcacaa aatcgaagat gcttgcgtaa aatcatggaa
     9241 agcagcggat ttcagtctta tcgctttgaa tggtggcact ataagttgat tgatgagcca
     9301 taccccgata cctattttaa ttttgccgtt tcataatgaa agtattggtt ttgtatcaga
     9361 ttttggattt gataaaatct atgctggtca tggacaatct tttactggaa agtggttaaa
     9421 gcatcttttg taatacattg caaattatag tattcaatct gcccagcggc agaaaagaaa
     9481 aaaaccgctg ttgggcagac ctttcccacg cccattttgt attttgctgg cggcggtttt
     9541 gagaaatcaa ggccgccgtt cttttctggc agcaaaaggt gtggcggccc ataggaggat
     9601 gccgctatgc tgttttgcta ttgccatgct ctaatccact tccaccactc aaaaaaagcc
     9661 ttgcccctcc acagggctat tccggttatg agtatgtact ataccatgta aatcagcagc
     9721 atagcaaagc attttctcgc ccggtaggtt tgcgacctgc cgggcgattt ttgtcgtctt
     9781 ttgcggcccg gctgtcccca aaaaaatttt ttgagaaatt ttcaaaaagg aggtatttag
     9841 cgggtagctg gcggccttgt caaagtcttg ttagcggaaa gggagagaac cacctaagtc
     9901 ccccacagaa agggggtgag aagatggatg ctatcccccg cgactatgaa gcgcggtgca
     9961 tgttcgatgc tttttgcaag acggtactgc gtaacgaggc caaaagctac ttggctgaaa
    10021 tgaagcgccg ccgtgaccgt gaagtgtcac taagctcttt gtcacaggca gatttggaca
    10081 agctctgtac agtagatcac taccccagcg acagctttac gttttcgtcg catggctatg
    10141 acctacatat caacaatgag cttgttgccg aagcctttgc cgcgctgccc agcatggagc
    10201 agagtatttt aatcctgcac tgtgttctgg atatggcaga tggtgagatc ggcggccttg
    10261 tgggaatgtc ccggagtgcc gttcagcggc acaggacaaa taccttatcg gagctgcgga
    10321 aacagctaaa ggcgttgatg ccgaagggag gttagacgcg gatgaagaac ccgaactaca
    10381 agggcattga gcttttgccc ctttcggtga tcgaggccgc ccgcgctggg gatgccggag
    10441 ccgtagagcg cgttttgcag tattacagcg gttacatgaa caagctgtgt acccggacgc
    10501 tctatgacga aaacggctat ccgcatgtgt gtctggacga ggccatgaag cgccgtctgg
    10561 aaatcaagct catccacgct atcgtcacga actgacgaac cggccccggc agcatgaaac
    10621 ggcttaccct ttccccgttt cggtgttttc ccggtggcct gatcgcacct tgataaatat
    10681 gcccgcaaga taacggcggc gcatcatagg gcatacggac acattctgtc tgtgccgagc
    10741 cggacggctg gtgcgccatg atacttttcc ctacgggaaa agcggagcga ggaacaccac
    10801 gccgcaatgt gagcgtggcg gcttgcgggc gacgacgtac aggcaggact atgatactcc
    10861 cttatagcca cagtccgagc gttaaaagcg tcgcaggcaa tgggtagggc cgcgtgagaa
    10921 ccacgcgggg gtgaaagtcc cgtggagctg tgccagcagc cgtccgtcta tgcccctaaa
    10981 tcacacaaac ctttgaattt gtgaaagggg gctgcattat gaaccatgat gtgcctattt
    11041 gggaaaaata cacgctgacg attgaggaag cgtctaaata tttccgtatc ggagaaaaca
    11101 aattgcgtaa gctggccgag gaaaatccgg cctctggctg ggtcattatg aatggcaacc
    11161 gcatccagat caaacgcaag cagtttgaga aaataattga cactttggac acaatctaat
    11221 agtgaaaaag ggccgcagat atgttataat aatttataag catatatgcg gctctttccg
    11281 tcatggaaag gagcatgacg agatgtcaga aaaaagacga gacagcaaaa atcgcgtttt
    11341 gcggtctgga gagagccaga gaaaagacgg acgatatgct tacaaatatg ttgatacctt
    11401 tggaaaaccg cagtttgttt actcatggaa gttagtgcct acggataaga ccccggcagg
    11461 taaacgggag gatatttcac tccgggaaaa ggagaaagaa atccagaaag accttgacga
    11521 cgggatagac accatcggca agaaaatgac cgtctgccag ctctacgcca agcagatcag
    11581 gcaccggggc aatgtgaagc acaacaccaa actgggccgg gaacggctga tgcgtattct
    11641 ggaacaagat cggctgggga gctgcccgat tgacagtgtg aagctgtccg acgcaaagga
    11701 atgggcgctt cggatgaaag aaaagggcct ttcttataag accatcaata acgacaagcg
    11761 ttccctgaaa gcggcttttt acaccgccat acaggacgat tgcatccgaa aaaacccgtt
    11821 tgacttccag cttagtgacg ttctggacga cgatacggaa ccaaaggtgc ctcttacacc
    11881 agcacaggaa gaaagttttc tgtcctttat tcaaggtgat aaggtttacc aaaagcatta
    11941 tgacgcaatc gttattctgc tggggacagg gcttcgcatt tcggaactgt gcggactgac
    12001 ggacaaagat ttggattttg aaaaccgggt aatcatcgtg agccaccagc ttttgagaaa
    12061 taccggcgtc ggttactata ttgacgaacc caagacccaa agcggcgtca gaaaaatccc
    12121 catgaatgag gaagtgtatc aggcgttcca gcgagtgata aaaaaccgca aaggtgcaaa
    12181 gccgttcatt attgacgggt atgccaattt cctgtttctc aaacagaacg gctatcccat
    12241 gacggcggta gattatggcg ggatgtttgg caggcttgtg aagaagtaca acaagagcca
    12301 tgaagaagcg ttgccgaaaa cgacgacgcc ccatgctatg cgtcatacat tctgcacccg
    12361 gt
//
LOCUS       DACCQI010000276        11678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.093, whole genome shotgun sequence.
ACCESSION   DACCQI010000276 DACCQI010000000
VERSION     DACCQI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.093"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..222
                     /locus_tag="IVO76_002935"
     CDS             <1..222
                     /locus_tag="IVO76_002935"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="catalyzes the addition and repair of the
                     3'-terminal CCA sequence in tRNA; these proteins belong to
                     the CCA-adding enzyme subfamily 2 which does not have
                     phosphohydrolase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7430838.1"
                     /translation="PQMDAVGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEAL
                     QLAEKAVVEQRIKNDKKQVLDFLSKEKLI"
     gene            376..831
                     /locus_tag="IVO76_002936"
     CDS             376..831
                     /locus_tag="IVO76_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430839.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            1193..1468
                     /locus_tag="IVO76_002937"
     CDS             1193..1468
                     /locus_tag="IVO76_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430840.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            1481..3559
                     /locus_tag="IVO76_002938"
     CDS             1481..3559
                     /locus_tag="IVO76_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7430841.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            3719..5605
                     /locus_tag="IVO76_002939"
     CDS             3719..5605
                     /locus_tag="IVO76_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7430842.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            5617..6564
                     /locus_tag="IVO76_002940"
     CDS             5617..6564
                     /locus_tag="IVO76_002940"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7430843.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            6584..7111
                     /locus_tag="IVO76_002941"
     CDS             6584..7111
                     /locus_tag="IVO76_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7430844.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(7174..7827)
                     /locus_tag="IVO76_002942"
     CDS             complement(7174..7827)
                     /locus_tag="IVO76_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7430845.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7959..8801
                     /locus_tag="IVO76_002943"
     CDS             7959..8801
                     /locus_tag="IVO76_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7430846.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8959..9855
                     /locus_tag="IVO76_002944"
     CDS             8959..9855
                     /locus_tag="IVO76_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7430847.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            9858..10571
                     /locus_tag="IVO76_002945"
     CDS             9858..10571
                     /locus_tag="IVO76_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7430848.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            10591..11109
                     /gene="msrA"
                     /locus_tag="IVO76_002946"
     CDS             10591..11109
                     /gene="msrA"
                     /locus_tag="IVO76_002946"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7430849.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            11106..11333
                     /locus_tag="IVO76_002947"
     CDS             11106..11333
                     /locus_tag="IVO76_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7430850.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            11485..>11678
                     /locus_tag="IVO76_002948"
     CDS             11485..>11678
                     /locus_tag="IVO76_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HAP7430851.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRF"
BASE COUNT         4060 a         1966 c         2418 g         3234 t
ORIGIN      
        1 cctcagatgg atgccgtagg agaatcctat gatcgtttac ctatcaagtc attaagcgaa
       61 ttagcagtag atggtaaaat cttgctagaa gaaactggaa aaaaacctgg taagtgggtt
      121 agtgaagctt tgcaattagc tgaaaaagcc gtcgtagaac aaagaataaa aaatgataaa
      181 aaacaggttt tggatttttt atcgaaagaa aaattgattt aaaaaacact agactgtatc
      241 tgctcagctt tgttggctac ttaaagtggg cagatacgtc attagaagaa aaaacataag
      301 atgtgaccac ttacataaat ttgtgctaaa ctgaggtagt acaagaataa attacatcat
      361 aaaggagtct ttttaatgag taaagaaatg acagcgctaa aattttattt tagaaatgga
      421 gaaacctgga cgatcaaccg ccgtcatatc ggcgatcttt ggatcaaaca aatcacaaca
      481 agttttggac gaatcaatgg cagtgaattt gttgaaatcc atccgtgtgc tggatttaaa
      541 atcgaaatct tccatgaagg ggacgcagtt gcgacacatg atatcaacct aggcggactg
      601 gaaatgggca tgtttaatcg cgcattgaaa tacgaagata tcgagcgaat ggaaatcctt
      661 taccgaaatg gcacacctga tcttgtgtac ttcccatatc tagataaagg aacagaagga
      721 ttagacaatc aatatcaatc aacaaaaatc agcgaaaaaa caggtaactt gtatatcgtg
      781 atcaatccag atcaacgtgt agaagatgta tacggggaat ttttcgaata aatcgatttt
      841 tacgcaaaaa tgaggctgtg aaagagatgt gtcttattac tactgatagg ctgtctcttc
      901 cacaccgttt attcagaata agggactgtg acaagatttt tgtcgcagcc ccattttttt
      961 gttgtgataa aagaacaaga aaggcaggaa tgggaaagta aacatccttt tgtcagaaac
     1021 ttttatcttt cttaagtttt acttgatatc tgatactatt tggaatagaa tgaggcgttg
     1081 aaaaatttat ggaaatttct aactagcaat aaacgatgaa acaatgaaat atttttcatc
     1141 caaaaagaac tttgctataa tgatggaagt ttgtgtatag gagatctgga aaatgagaga
     1201 gagttttgaa caacaaaaaa aactgttgca tgatcgctat ggcgcccttt cgatggatga
     1261 ccgccgtcaa atactttgta agttgcgtaa aagaaatata ttgatgtatc gtcagttgga
     1321 acgtctgaaa catgatctgc ttcgtttgga gtcaaaacgt gtacagtgtg aattagaggg
     1381 gaaccaaaca caggttgaag tagtcgaaac caaaatttta aagaaaaaag aacaattttt
     1441 aaaaatgctt acacaaaata aaaaataaag gggatggacg atggaatttg ctgagttagt
     1501 tattgtattt gcagtaacga tcaccttatc gaacatagct agccgcattt tgccaatgat
     1561 tccagctcca ctgatccaaa ttttccttgg agtggtatta ggtctgacag agtggggaca
     1621 atcaatcgat tttgaacctg aattgttctt agttatgatt attgcaccat tgttatttag
     1681 agaaggagaa aaagcagata tttcttcgat tctcaaaaat ttcgggacaa ttttgttctt
     1741 ggcatttgga ggagtcattt tgacccttgt cggggtaggt gcgacgttat cattcctgtt
     1801 gccaagtgtc ccacttgctg cctgctttgc ttttggagca gcgttaggtc cgacagatgc
     1861 ggtagcagtc agttctcttt ctggacgagt gaatatccct aaaaaggcga tgcatatttt
     1921 ggaaggagaa ggtcttttga atgatgcatc tggtgttact gccttccagt ttgctcttgg
     1981 tgcgctgatc accggatctt tttcagcagt aaacgcgggg atgagtctag tagtttctag
     2041 tattggggga gctttgatcg gctttttgct tgtctggttc aaacaaaaaa taattcattt
     2101 gatcgaaaaa gcatcagcac aagatgtcac agggtatctg ctgatcgaac ttttgcttcc
     2161 ttttctagcc tatgtattgg cagaggcatt tgatgtatct ggaatcattg ctgcagtggc
     2221 agcaggtatt ctccaagctt ctggttttag gaaaatctct gtgtttgatg cagaattgtc
     2281 gagcttgtcc cacagtacat ggacaactat tgcttttaca ctgaatgcat tagtatttat
     2341 atttttagga atcgaattga cgcaagtgtt ctcgccagta tggggagacg gtttatatcc
     2401 aaacggtctt ttattagcga ttatcgtatt aatctctgtc atgctttttg tgatcagatt
     2461 catttctatc agtctttttt atgtattcaa agatggcagt aaaaagttca aaaaacagtt
     2521 gaacgagatc ttgatcttaa catttggcgg tgtaaaggga acagtcagtt tggctacgat
     2581 ttttattttg ccattttcaa tcaataatat gatgttttac cagcggtctt tgcttttgtt
     2641 tctgacggcg ggtgtcatct tagtgacttt ggtcataggg atcattgtct tgcctatgct
     2701 gacggaaaca gaggaagcga aaagcacgga tttgaatgct ttgatgattt tagaagaggt
     2761 cgttgaagtt ttgcgaaaag aaataaagga aattgatcgt aatacaaaag aatttttagc
     2821 cactgaagca gtgatagaaa attatcaaga acggatacgt gatctgtact tggaagattt
     2881 aaccgatgat gaaaagcaag aagtacaaga aatacaagca ctgattttgt cgatcgaacg
     2941 ggatggattg gatgaaagtt atcgttcggg caagctttct tctaatggtt atcgttttta
     3001 ctcccgtttt ctttctcgat tcgaacattc tatcacaagc cagattttgt cttttatcgg
     3061 cttttggttc attgttgtcc gtaggttaat gcgaatcgtt ctccatccga aaatgttttg
     3121 gcaaagaaga tatgctgatc gtcagacatt tattagtgat gaagatatcc aagaaatcag
     3181 agagacatat caaaaaaata ctcagttgat tattgaaagt ttagataact tgactgatat
     3241 ttatgatgaa acgatggttc aattttttat ccagcaacgc aaacttgaag gaataaaaat
     3301 gaactcgggg aatttgattt cttcgtggat gatccagcaa gatagtctgt ttacgaaaaa
     3361 aatgctaaga ggttattatc ttgaaagaaa aatcattgat gaatacgaag ttgctgaaaa
     3421 aatcacgacg ttttctgcga acagctatcg caggaatatc aatttgttag aatcttatac
     3481 gatgaacaaa ccatctgata acttttcttt ccgatttgcg tttcgagcaa agtcaaaaaa
     3541 aacgagtcca ataaaataaa aaggtaaaaa ttttcaagtt ttataaaaaa caaaacatat
     3601 cagaacagga tcgaatcggt cctgttcttt ttttgagaac acaaaaagaa caaataccaa
     3661 aaaaattttc ctgaagttca ctatttttat gatacaatat ccaagataag gagggaacat
     3721 gttgaaagaa ttaaaagtaa ctgacctcaa aaaaacttat ggtgaaaaag atttgtttga
     3781 ccagatttct tttttggtcc acgaaaaaga ccgtatcggg ttgattggga cgaacgggac
     3841 gggtaaaaca agtctcttaa atattattgc cggtatcgat agcagcgacg gtgatcggca
     3901 aacagtgttt tatccaactg attaccgtat tggttatctt tctcaagcag ctgaattttc
     3961 cgatgaatta actgttctcc aagcagtctt tcaaggaaac agccccttga tccaaactgt
     4021 tcgagcatat gaggaagcat tgatcgaact aggggaaaac ggagaagacc ctgatgtgca
     4081 aaaacgttat gctaaagcag aagaacaaat gaataaagaa gatgcttgga ctacagatac
     4141 caacgcaaag attattttgc aaaaattagg aatcagtgaa ttagataaaa aaatcagtac
     4201 tttatcaggt ggtcagaaaa aacgagttag tctggcgcaa gtgttgattg atgaacccga
     4261 tttactatta ctagacgaac caacaaacca tttagattat gaagcgatcg agtggttgga
     4321 aaactatttg aaacaatacc gtggcgcact attgatggtt acccacgatc gttattttct
     4381 agatagagta gcaaaccgaa tcttcgaact ttcatttgga aaattatacg agtataaagg
     4441 aaattatgaa gcttacgtct tagaaaaagc agaacgtgat cgtgtagaag tagaacaaga
     4501 agaaaagcgc aagcggcttt ataaacagga attagcttgg atgagggctg gcgtacaggc
     4561 acgtggcacc aagcagcaag cacggatcaa ccgtttcgaa gatctaaagg agaacttgta
     4621 ccaagtaaac caagaagatg atttagagct gaacctagcg acacaacgtt tagggaaaaa
     4681 agtactggaa atcaaagatg gcagttaccg tataaatgac caaacactgc tagaacattt
     4741 agatttattg attcaatctc gggaaagact aggtattact ggtaaaaatg gtgcgggaaa
     4801 atcgacattg cttaatattc tagctggcag aattcccctg gatagcggta caatgtctat
     4861 cggtgaaacg gttcatcttg cgtattatac acaagaaaat gaagaaatgg caccagataa
     4921 acggatgatt gcttacttac aagaagctgc tgaagaagca aaaacagcag atggcagtca
     4981 aatcggagta gcagaattac ttgaacgatt tttatttcca agatttatgc atgggacact
     5041 gataggcaaa ctttcaggcg gagaaaaacg ccggttattt ttgttgaaac tgctgattca
     5101 gcaacctaat gttttattat tggatgaacc gaccaatgat ttggacatag caacattgac
     5161 gattttggaa gattatttta gaagttttcc aggtgctgtg atcactgttt cccatgaccg
     5221 ttatttcttg gacaaggtag cagataagtt attagtattc caaggaaacg gaaaacaaga
     5281 actttattac ggaaacatgt ccagttattt actgaagcaa aaagagaccc agcagcctgc
     5341 tgaaaaagcg aaaccaaaaa tacagagcaa ggaacctgct gaaaagaaga aattatctta
     5401 catggaacaa aaagaatggg aaacgattga agatgagatt gcagaacttg aagaaaaaat
     5461 ctcattgctc caagaagaga tgaatcatca aggagataat ttcactcgat tacaagaatt
     5521 gcaaaacgac gtatctgaaa cagaagcaca acttgaagaa aaaatggcac ggtgggaata
     5581 cttgagtgaa tgggtagaag attaggagga aaaaggatgg aacaagcgta tcttgactta
     5641 ggaagaaaat tgttggaaga agggcatcgt aaagaagaca gaacaggcac aggcacatta
     5701 agcttattcg gataccaaat gcggtttgat ctgcaaaaag ggtttccttt gttaacaacg
     5761 aaacgagtac cttttggatt gatcaaaagc gaattgcttt ggtttttaaa aggagatacg
     5821 aatatccgct atctattaca aaataaaaac catatttggg atgaatgggc atttgaacgc
     5881 tacatcaaaa gtccagacta taatggacca gatatgactg attttggcca tagatgtttg
     5941 acagatcctg aattcaatga agaatataaa aagcagagca agcttttttg cgagaagatc
     6001 ctaaccgatg attcctttgc tgaaacatac ggcgatcttg gacacatcta cggctatcaa
     6061 tggcgtcact gggaaacaaa agacggtggc tttatcgatc aaatcaaaca agtgatcgaa
     6121 gcaatcaaaa agactcctga ttctagaaga ttgatcgttt ctgcatggaa tccggaagat
     6181 gttcctagca tggctttgcc tccttgtcat acgatgtttc aattttatgt tcaagaggga
     6241 cggctaagct gccaattgta tcaacgcagt ggggatgtgt ttctaggcgt tccgttcaac
     6301 attgcaagtt atgcgttgct gactcaccta attgcacatg aaacaggttt agcggttggc
     6361 gaatttgttc acacattagg agatgctcat ctttatatca atcatatcga acaaatgaaa
     6421 gaacaattga gccgagaaat acgcgcattt ccgactttag ttttgaatca ggaaaagcag
     6481 tcagtatttg attttgagat ggaagatatc caattagaag gatatgaccc acatccggcg
     6541 atcaaagctc ctatcgctgt ttagaatcag aaaggatgag aaaatgttag ctgcaatttg
     6601 ggcacaggat gaaaacggat tgattggaaa agaagaccgt cttccttggc gtttgccaaa
     6661 tgatctgaaa ttcttcaaac aaatgacaga agcaaataca ctagtgatgg gacgaaaaac
     6721 attcgaaggg atgggtagcc gtccgttgcc gaatcgtcag acgattgttt tgacgagaga
     6781 ttcttcctat aaagcagaag gcgtgcatgt catgcatagt gtggaagacg tgcttgctct
     6841 ggaaaaagaa acagacggta tctttttcat cgctggaggg tcagctgttt atcaagaatt
     6901 tctgccattt tgtacgatat tataccgtac agtcatccat catgtattcg atggagatgc
     6961 ttattttcca cctgttgact ggtctgattg gtcactgatc aatactagtc aaggagaaat
     7021 ggatgaaaaa aatcgttatc cgcatcaatt tgaaacgtat cagcgtaatg agaatcctgt
     7081 aaaaagcggg acaggcgaat tgacgatata aaaaagctat ggatagacta gctgttattt
     7141 agagctgtcc catccatagc tttatttttt ttattaagta aagaataaaa cagagaagta
     7201 cataaaacct gcacctaaca taacaaacaa atgccaaaca acatgcatat agcggataga
     7261 attcaagcta taaaagaatg cgcctatcgt gtaagaaagc ccgcctgcta ccatcaaaat
     7321 aacaccagtc attccaagag agtgataaag aggaacagca gccacgacac agagccaccc
     7381 cataaaaata tagatgatcg tagatacttt tgatactctc tcccgtttat gcaacgtcaa
     7441 cgctttatat ataatgccaa gaatcgcaaa caaccagatt aatacaaata aagtccaacc
     7501 tagccatcct cggatactca gcaaacaaaa aggagtataa cttcctgcaa tcaaaagaaa
     7561 aatcgaacta tgatcgaata cttggaacac cttttttgct tttgtaaaaa tcaatgaatg
     7621 aaacaaagta gacgttaaaa ataaaagaat catcatcgat ccatagatag cataagagac
     7681 aatatggagg gaggaatgca agtgggcacc ttttactaaa agaaccacta gtcccgcgat
     7741 actcaatcca gtcccaattc catgggtgac tgcattaaac acttcattta atatttcata
     7801 cgttcgatta aatttcgttt tttccatgaa aacggctcct ttactaggtg taattaatga
     7861 aaaatcatta tttgtctgtt atactaaaaa tagaaattgt cttacatgta gtttaacatg
     7921 aataaggcat cttgttaaag aaagagagag agaaaataat gacaaatgta aaaatcgtaa
     7981 cagattcttc atgtacgatg gaaaaaaatg tacgcgatgc tttaggcatt cgtgtcatgc
     8041 cgctatccat catgatcgac ggaacagtat atcccgatga tgaccaacta gacggcgaaa
     8101 gatttatgga aatgatggcg aaagcaaaaa atctgcccaa aacaagccaa ccaccaattg
     8161 gagaattcat ctcgatgtat gatgagctag gagaagacgg cagtgaagtg atttcgatcc
     8221 atatgacaaa aggcttgagt ggaacagtag aagcagcaag acaagccagt aatctaacaa
     8281 aaacgaaagt aacggttatc gacagtgata ccactgacca aggattatct tttcaggtaa
     8341 ttcgagcagc tgaattagca aaagaaggta aaacagtaga gaaaatcctt gaagaagtca
     8401 aaaaagtaca agacaataca aaattataca tcggaatatc cacactagat aacttggtaa
     8461 aaggtggaag aatcagccga acaactggtt tgctgtctag tgttttgaac atacgcgtgg
     8521 tgatgaattt cgcccattct gaattaatcc ctgtaactaa ggggcgagga aagaaaacgt
     8581 tcgataaatg gtttaataat ttgaaaaatg aactgaaaga attgccaaat gtgcgacaaa
     8641 tcggtatttc acatgcggat gcaaaagaat tggcgcaaga attcaaagag ggtttgcaag
     8701 aattattccc ggacatgcat atccctgtac tgcatacgaa tccggttatc gctacgcata
     8761 ctggtaaaga cgcgtttgca atcatgtatt atgtagatta gtcgaaaaga ggataagccc
     8821 tcagcctgaa aggcttgcag cttattctct ttttctttat aaaattagga aaataattgt
     8881 tttctttttt cataaagact acctttttga taaaattttt gataacatta gaaccaatcg
     8941 aatacaagag gtgaaaaaat gaaattaacg aaacgacatt tgatcctcgt tttgttccca
     9001 ttattaattg cgatcatcat ctatgcaggt ttagctgctt ctataccgaa agctggccca
     9061 ctaatgaaac cagtaactac tgtgagagaa gatccaaata aaaaagaaaa acttcactac
     9121 atagctatcg gagattcgct gactgaagga gtaggagatc aaaccaagca aggaggattc
     9181 gttccccttg tcgcaaatga tctaaaagac cgttataatt tgaccgcggt tgaaacagaa
     9241 aactatggtg taaatggtga acgaagtgac cagatcttaa agcgagtaaa aacaaaagaa
     9301 gaaattcgaa acaatatcaa aacagctgat tttatcacac tgactgttgg cggtaatgat
     9361 ttgatgaaag ttatccaaaa taatttattc gggttaacgg tcaattcctt taaaaagcca
     9421 ctgaaaaact atcaggaaaa tgtcacagaa ctattggaag aatttcgatc cctcaataaa
     9481 accgcaccta tttacatttt aggcatctac aatccatttt atttgaactt tcctgagatc
     9541 actgacatgc agacaattgt taacaattgg aacaaaggaa cagaaacggt gacaaaaaaa
     9601 atgccgaact gctactttat cccaatcaat gatttgcttt atcaaggatt gaatcaggaa
     9661 gtaggtgtca cagaagaagg aacagatcaa acaactagca cgacagaaaa tctaaatggc
     9721 atcaaaaata atgtgctcta tgaagaagat catttccatc caaataattt aggttatcaa
     9781 ttgatggcaa atgcagtgag agatcagctg atcgagacac aaaaccaatg gttagagaag
     9841 gaggatgatc aataaacatg aaaacaagat ctgacgtatc ttcaaacaaa acaaagaaaa
     9901 caaattactg gaaattcgcc ttccttatcc ttcttggggt agtacttggt tcgaccgtat
     9961 ttttaggcag ccgtattttt gctaatcggg aaccagaact cccagaaata ccagctctta
    10021 cggaaagaca aggagatcct gtcttaacga tcaattcaaa taaagaaaaa gtcaatcaaa
    10081 tcatcagctt tttccttagt gagtatcaaa aagatagtga tattgaatat aaattttatt
    10141 tggaaaatga agcactactc aatggtactt tcgaagtact aggttttcct atcgatttct
    10201 atttatattt tgatccgtat gtgatggaaa acggaaatgt ccaactaaaa gctagaagtc
    10261 tttctatcgg aacattgagt ttgccaatca aagatgtgat gaatatgatc aagagaaact
    10321 ataaattacc agattggatc gaagtcaata cagatgatct gactatcatg cttaggttgg
    10381 atcaattccg aatgcaaaac gggatgtata ttaaagcaga taaaatcgac ttagtaaatg
    10441 atgacattcg gtttagtcta tatttgccaa aagatacctc tgatgcttca aacaactcaa
    10501 ataccaacaa aactagcacc agcagtactg aaagcaagac aaaagaaaat aaaaaatcat
    10561 cggatgagta gtattagagg gggaatcata atggaacgag caatatttgc aggaggctgc
    10621 ttctggtgta tgatccagcc tttcgacacg cttcctggta tccatacagt gatgtcagga
    10681 tatactggag ggcatgtacc agatccgact tatgaacaag taaaaagcca aacgactgga
    10741 catacagaag cagtggagat tatctatgat ccaacattga tttcatatga acaattagta
    10801 gaactttact ggcagcaaac tgatccaact gacgctttcg gacaattcga ggatcgcggt
    10861 gacaattatc gcccggtgat cttttatttg acagaagaac agcggatcat tgctgaaaaa
    10921 agtaagaaac agttagctga aagtggacgt ttcgaacagc caatcgttac aaaaatcgaa
    10981 ccagcccaac cattttatct agcagaagaa gaccatcaag agtattataa aaagcatcct
    11041 gaaaatttta aaagaaacca tgcacgccgg gctgccttta tcgaagaaaa ttggaggaac
    11101 gaagggtgaa tcgaagcttt tttcattatt taatgacact gcgaggcggt ggcacacatg
    11161 atgctttatc tgttttcgct acagaagccg gtaaagacat ccaatttcct aagcacagct
    11221 ctagctacga agaaatttct aattatctgg aactgaatgt cgactatttg ccgtccatgg
    11281 atatctttga cgaagcatgg gaaaaatatt tagaaaataa tcatgacata taagagatag
    11341 ttttgtactt tatgatcaaa aaagtttttt gtccaatagc ctaattgaaa gaattatttt
    11401 tatttcagct atctagtcgc atttttttga tatttttctt ataataaaga aagaatgaaa
    11461 aaaagaggga tggattgata gagattgaca aaaaaacaac gctatattaa tcgcttctgg
    11521 cttgtattca aatacttact tgtttctgta ttccttgcgt tcatgttgcg gggattttta
    11581 ttcatcccgg taccagtgga aggcaattca atggaaaacg tgttgaagca aggtgatatg
    11641 gttgtgatgg aaaaattttc ggagatcaga cgatttga
//
LOCUS       DACCQI010000277         7438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.127, whole genome shotgun sequence.
ACCESSION   DACCQI010000277 DACCQI010000000
VERSION     DACCQI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.127"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(80..1420)
                     /locus_tag="IVO76_002949"
     CDS             complement(80..1420)
                     /locus_tag="IVO76_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7430852.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1689..2585
                     /locus_tag="IVO76_002950"
     CDS             1689..2585
                     /locus_tag="IVO76_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7430853.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(2735..3676)
                     /locus_tag="IVO76_002951"
     CDS             complement(2735..3676)
                     /locus_tag="IVO76_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7430854.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            3802..4923
                     /locus_tag="IVO76_002952"
     CDS             3802..4923
                     /locus_tag="IVO76_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7430855.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5181..6683
                     /locus_tag="IVO76_002953"
     CDS             5181..6683
                     /locus_tag="IVO76_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP7430856.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            6750..7277
                     /locus_tag="IVO76_002954"
     CDS             6750..7277
                     /locus_tag="IVO76_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7430857.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2505 a         1359 c         1486 g         2088 t
ORIGIN      
        1 caacaagact tttgtcacaa ctcctttctt caatagctcc atctattcca cataaataaa
       61 ataaaatttt ctatcgattt cattcattcg tcaatacgaa cgcatcattc tttttttgat
      121 aactgaaata aatgaacagg taaagcaggc cagtcaatac agttacccca atggagttcg
      181 tgaacaggat accaaggaaa atcaatccta agacagctaa tgcaaaaaag ctgattcctt
      241 tatcccataa tgttcctttt cttagtgcca gtacaaagga tgccgcactg ataatgatcc
      301 agatcaataa tactgtatac gaaagggaac ctgccagata gccaaataat ttgtcaccga
      361 caaaataaga aagagctaca ccaagataaa gaacactggt acaaaataaa acagcacgtt
      421 gcggtacttg atgcttgttt aaaacagcta gttttctaga ggaacctttt cgatccttca
      481 aacggaagaa caaaattctt gaagatgcat acacacccga attgatagat gagaacaaag
      541 caaggacaat cacaaaattg acgatatccg ctgcaaatgg gatattcatt ttttcaaata
      601 ccatcacaaa aggactgaca ttcgtgcctg ctaaagtatt ccaaggaaaa agaatcaaaa
      661 gcaaaaacaa aggaatgatg taaaaggaaa tgattcttcc catgacacct ttgattgctt
      721 taggaatcgc acgtttagga tcatctgctt cgcttaccgt gatagcaatc agttcagaac
      781 cgccgtaaga atagataact acaagcaaag aactgatgat tcccttcata ccatgaggag
      841 taaacccacc atggtcagtc aattgctgaa gggtagggaa aacgcctgtg cccaacattt
      901 gctgaccgac aagataagta gcaaaaatga tcagtaagat aatgacactg attttagcaa
      961 aagccagcca atattctgtt tctgcgaata accgtaccga ataaagattg atgatcgtag
     1021 ttaaaacagc caacataaaa acaaataccc aggctggaat gtgcgggaac cacaactgca
     1081 aaaaggaagc agctgcaaca gcttcagcga tgatattgat aatccacata gaccagtaga
     1141 cccaatcagt aaaatcagca gaatgagcac ctagataagg gcgtatcaaa ccagataatc
     1201 cgtgatgcgt gtcgccaccg cttaaaacga tttttcccag acctttcatc acgatatata
     1261 aaatcagccc accaatcaaa aaagcaatca atacagaagg gccggcgatc ccaatcgctt
     1321 cgccacttcc tttaaataga cctgcaccga ttgctccacc taaagctaac atggtgatat
     1381 gtctggagct aagttgtttt ttcaattttt tatcttccat aaatgacctc tttcttttaa
     1441 attcacaatc aaaatttata ttaagaataa atagcaatat ttaacaatga ctaatgataa
     1501 caaattttca gacaattaca actattcaga aaacttttag gcgaatttta atagaagaat
     1561 gatttatttc atcatatttt catacaaatg ttaaattttc atacaaatat aggaaacgtt
     1621 ttactggctt tttactattt aaacaggcta tcataggaaa aagtgacaga gaaagtgagg
     1681 cggaaaagat gaacaaaaat gacgtactgc tagttgcaaa aaataaatca ctttattttt
     1741 tgccttccat ccgctcttta gatgtttggt ccggaaaaga tttactacag tcggaattca
     1801 ttaaatcagc tagacaacag ttggcaaatt atcagctgat cgttttttta gattatggat
     1861 ttgatatgga gatggctcga cgcgtgcgcc catttaccaa tgcaaaaatt gtgttatttt
     1921 tttggaatca cttcaaagca gaacacgagg cgatgctgaa gcaggcacaa gatgaaccgg
     1981 caatcgatga gatttatcat tttgattttt tggaagcaaa agaacttggg ctgaaacata
     2041 atagttcatt ttattcgaaa catatgaatt tttcttctag cgaaccaaag acggatctat
     2101 tttttggtgc aacgaataat gggagaaaag aacgagcaga aagctataag caggaattta
     2161 taaaaagaga tgttacgacc aactatttca ttcttccaca tcgagggaat gaacaacctg
     2221 gatatcttac ttatgaagag tacctgaaaa aaacagggga atcgcgcggg attctggaac
     2281 tgttgcggga gggtcagcaa ggagtgactt tgcggacatt tgaatccatg tattttcaaa
     2341 agaaactagt gacggataat caagcgattg ctcattatca tttttatcgt cctgaaaata
     2401 tttttttgct tcgagaacgt gatttagatg aattaccgga atttctgcat acaccattta
     2461 agccaatcct atcagagcta ttggaatttt atgatgcaga aaattgggca aaacgattta
     2521 tcactaacga ttccactatt tatgagcaat acgaatatga cctctcctct gttgaaaaca
     2581 aataaaaaac ctacattttg aagagattat gtactgaaac aaacaagcac aattcttatg
     2641 gcatcatgag agttgtgttt tctttttgat cttttattgg gcttaaaaaa agagaaccag
     2701 ctgctgattc agctgattct catcagagag ccttttattc tgcgccaagg attccttctt
     2761 ttgcatggac taattgggtc aaaaaggcac aatatggtgt agcaatatca tatttttgtc
     2821 cttttcgcca aatggcacca ttgatgtagt cgatttcagt caaacgatgg tttttgatca
     2881 ggtcttggta catagatggg taatgcaacc cgatgttgtc aggatcataa gtagaagcaa
     2941 tatgttggta cacttcttct tgatctagta tgattccttc ttttgctgcg atagaagcaa
     3001 attcagatac gattgttcga accatgctct ctgccgcttt ttgcgcgcct agttcagcaa
     3061 tattacaatc caaaatcgtg cataaaccat tcaatgttcc atttacacaa gcttttcgcc
     3121 aaatggaata acgaacgtta tggctataga tcggatttaa atttgcttct tgaaagactt
     3181 ctacaacttt ttttgtaaat gcttcgcctt ctggttccaa gttctctagt tcgatttctc
     3241 catcacctaa caatgtgact tttccgggac cagctagacc agctgtccac atcgtgatcc
     3301 caaataagat atttttcttt ggcacatatt tttccagtac atcttcatga cctaatccat
     3361 ttagtaaaca caaaacataa gtattgtcag taataatgga ttggatcgaa caaaacatat
     3421 catctaattg attggctttt gttaatgcta cgatcaagtc cacgtgttcg ttgctttcga
     3481 tgatttcttc tggtgaatag attggcagtt ttgcgaccac ttctttaccg ttaaagtcag
     3541 caatcaaccc atttgtacga atggcttcaa tgtgagcagg ccaacgatcg atcaagagga
     3601 cctcatttcc gctttgatgt agcatcaatc cgattcggct gcccattgct ccggccccag
     3661 ctattgcgat tttcatgttt tttctctcct ttattcaatg agttcccttt tagtatatag
     3721 aaaaatggaa gaaaatagca ttgttttgtt tgaaaaaaat gaaaaaaagt gtcttactta
     3781 agaagaaagt gagggataca gatggatatt ttggcggcaa ttgattcatt taaaggaagt
     3841 gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct gggaagatcg taaaggaaga
     3901 aaaatcaatg tgccgattgc agatggcgga gaaaatactg cagaagcaat ctattacgcg
     3961 ctcaggggaa catggcgtac cattgatggg atggatctct tattccgtca gcgaaaaatc
     4021 agctatttat tgacaacttg gcaaggaaaa aacaccgcag tcatcgaatc tgcacaagca
     4081 ttggggttag atctacttga taaaccaacg gatcaaacta tccgacttgc ttccagttat
     4141 gccttaggag aacttattaa agatgcactg cttcaagaag tagaacaaat tattgtcact
     4201 ttaggaggaa gcgggtgtag cgatggtggt ctaggattac tccaatcctt gggtgcttct
     4261 ttagaagggt acacagaagg aaatccttta ttatctacta aaaaacttca ttttgatcga
     4321 agaaacgttc ttgtttctaa agtaaaattg attatcgctg cagatgttac caatccatat
     4381 acaggtcaac aaggagctgc ttggcaattt ggaagacaaa aaggaggaat agatacgacg
     4441 ctagctttct tagataaaca agcaggaaag attgctcagc aaataaaaga tttctgggag
     4501 gtggatttag atgatattcc aggaagtgga gcagcaggcg gtattggtgg agcccttttt
     4561 ttgttgggag cgaagatggt ttctggtttc gatttagtca gccaaatcac tggattggaa
     4621 aaaaagatcg aacaagcaga tgtcatcatt acaggagaag ggcggatcga tcagcaaacg
     4681 atccacggaa aagttccata cggtgtagca gtgcttgcaa aaaaatatcg aaaaccaata
     4741 atcgctattt gcggaagtag agaccaagag ctagaaggac tttctgattt tctcccaatt
     4801 gtcttaagta ttcatttggg tccaataggt ttagaagaag caatggaaca caggcaaaca
     4861 ttagaaaaaa caaaaatctt aggacagaca cttagcagat ttttcaccat cctcccgaag
     4921 tagttgacaa aatcattttc tttatgctat cgtaatttca aatataggac aaagtgaggg
     4981 atccgtgcat tgaaaaatta agtcgattga tgaaagctgc attcagactg taccttgtgg
     5041 agacaaggaa gcagtgtgca gattttcggg attggcttgg ttttttcgat gtatgggtct
     5101 ttttgtgtgt atccaaccct tgcatcctct ttagccagtt ctgaaaatag gactggtctt
     5161 tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa aaatctgaca ttcggctacg
     5221 acagccaagg cacattatta tttgaacaag caaatctaaa ttttgacaca caatggaaac
     5281 taggacttat cggacgaaac ggtcgaggaa agacaacttt actgaatatt ctacaaaaca
     5341 aactacctta tcaagggcaa gtaatccatc agcaagaatt tgcctatttc ccgcaacaga
     5401 caaaagataa agaacgttta acctattacg tgttaaatga tattacggat tttgagatat
     5461 gggaaatcga aagagagctc caattgatgc aaacagatcc tgaaatctta tggagagaat
     5521 tcagcacact atcgggggga gagaagacaa aagtcctact ggcactttta tttgtggatg
     5581 acactcattt cccgttaatc gatgaaccaa cgaatcattt ggatatctct ggtagaaaac
     5641 aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat cgtggtcagc catgaccggg
     5701 gatttatcga tgaagtagtg gaccatgttt tagcaatcga aaaaagtcaa ctggaacttt
     5761 atcaagggaa tttctctatc tatgaagaac agaaaaaact tcgtgatgaa tttgaaatgg
     5821 ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa gaaaacagca gctgaaaaag
     5881 ccgaatggtc tcgttcccga gaaggagata aaacaaagaa acaagtcgga ttcatcgata
     5941 ctgaatctag acgagtgaat aaaggagcag tgggtgctga tgctgcacgg acgatgaaac
     6001 gatccaaagc aatcgtgaat cggatggaga cccagatcag cgagaaagaa aaactattaa
     6061 aagatatcga atatatcgat tctttgacga tgaatagcca agcgtctcac cataagcgac
     6121 ttttaagcgt agaagatctt caattagggt atgaaaatct gttattcgag ccaattcatt
     6181 ttacaatcga gcctcatcag cgggtggcga tttcaggtcc taacggtgca ggaaagtcat
     6241 ccattatcca ttatcttctg ggggcattca acggcaaggt tataggagaa aaataccagc
     6301 caaaacatct gagcattagt tatgcaagcc aaaattatga agacaatcga ggaacgttgg
     6361 cggaatttgc agagaaaaac caagtagact accaagcatt tttgaacaac ctccgaaagc
     6421 ttgggatgga aagagatgtt tttcataaca agatcgagca gatgagtatg ggccaacgga
     6481 aaaaagtgga attggctaaa tctttatcac agccagctga actatataca tgggatgaac
     6541 cattgaatta tttggatgtc ttcaatcaag aacaattaga acaactgatc ttgaacgtga
     6601 aacctgccat gttactagtg gaacatgatc aaaccttcct ggataaagta tctactgaga
     6661 ttatttctct tgagagaatc taacccttgt aatctcaaga acaacaaggt atggttaagg
     6721 ggaactttgc caacgaagga gagaatcaca tgctttatgt aacgagacac ggagaaacaa
     6781 cttggaacgc acaaggatta gtttgcggac gggcagatgt gccgctgact gaaaaaggac
     6841 agatgcaggc acagaaacta gcagaaaagg tagtagattt gcctgttcca atcacgaaaa
     6901 tcatccattc ccctcttcaa cgagcaagag atacagcaca agcagttgct gatcgtctca
     6961 gcttgccgct gaccgttgat gaacgattgg tagaaatgga ttttggggat tatgatggac
     7021 tgccaagcaa agatgagaac tttcaaaaag cacgtttagc gttcgctgta cgattcccaa
     7081 atggggagtc agtcttagat gtgtatgcac ggatcgttcc tttattgaaa gaatgcatcg
     7141 aagacgaaga aaatgtctat ctgttagttt gccataatgc cttgataaga gtgatcaatg
     7201 cttattttca tccgatgcct aatgaggggt tctttacatt catggtagat aatacagaat
     7261 tggtttctta tgagtaatta tgtttttgtg ttatttaaat aaaatatttc aaggcactgc
     7321 ttttgcttag cagtgccttt tttaggttat gacaattctt ttgataatat aaactcaacc
     7381 tttagaaatg aaatttaatt attttaataa aattattgaa attttgtttc tttagata
//
LOCUS       DACCQI010000278         6777 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.136, whole genome shotgun sequence.
ACCESSION   DACCQI010000278 DACCQI010000000
VERSION     DACCQI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.136"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IVO76_002955"
     CDS             <1..382
                     /locus_tag="IVO76_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7430858.1"
                     /translation="EIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQIHDD
                     YRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDETQEG
                     SLERYKKDSYYWYQKLIEENGENL"
     gene            571..3018
                     /locus_tag="IVO76_002956"
     CDS             571..3018
                     /locus_tag="IVO76_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7430859.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            3248..4315
                     /gene="rlmN"
                     /locus_tag="IVO76_002957"
     CDS             3248..4315
                     /gene="rlmN"
                     /locus_tag="IVO76_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7430860.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(4508..5611)
                     /locus_tag="IVO76_002958"
     CDS             complement(4508..5611)
                     /locus_tag="IVO76_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7430861.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            6164..6712
                     /gene="aac(6')"
                     /locus_tag="IVO76_002959"
     CDS             6164..6712
                     /gene="aac(6')"
                     /locus_tag="IVO76_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7430862.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2276 a         1164 c         1460 g         1877 t
ORIGIN      
        1 ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc gttaccgcat
       61 gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag aagataaaca
      121 aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca tcaaagaagc
      181 aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag atttgcttag
      241 ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag acgaaacaca
      301 agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa aactgattga
      361 agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg ctggatatgg
      421 ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa taagctgaaa
      481 aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt ttttttgtat
      541 aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc tactaaaaaa
      601 aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat gctgacgatt
      661 ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt aggtttttta
      721 ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca aatcctctgt
      781 cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt ttcaattggt
      841 aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt attgttacat
      901 gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac gacatgggat
      961 ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg tgggatgatt
     1021 ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag ttatttagca
     1081 gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg ctatcagctg
     1141 ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg tgatcctgcc
     1201 aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc agaagcgaaa
     1261 gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag aagctgcaga atatgagaag
     1321 aaaaaagcgg tgagccagca aagggaaaat actgctgatg aatggcagga accagaaaat
     1381 acagagccgg agcagctaag ttttgttcca atcgacagtt ttcaagaaaa tatccatcct
     1441 gcgaatctag agaaaccagt accagataca ccaaaacaga ccaatacagc agaaggtttt
     1501 tcagatgagt tgccagagga tgacggtacc tcattagaat ttgagattga agcagaacag
     1561 gaaaatcaag actatgaatt accgacagtt gatttgcttg attctatacc aactgtggat
     1621 caaagtgatg agtataaaaa aattgaaaaa aatatcggcg tattagaaca aactttccaa
     1681 agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag gaccatccgt cacaaaattc
     1741 gaagtccagc cggctgtagg ggtaaaagta agtaaaattg tgaacttaac agatgatatc
     1801 gcgcttgctt tagcggcgaa agatgtccgg atggaagcac cgattccagg taaatcactg
     1861 attggtattg aagtaccaaa tggaaagatc agtatggttt cctttagaga aatcatcgaa
     1921 gcacaaccaa accacccaga taaattacta gaagtacctt taggacgtga tgtttctgga
     1981 cgggtacaaa cggctgatct ttcgaagatg cctcacttac tcgtggctgg ttcgactggt
     2041 agtgggaagt cagtagcaat taatgggatt atcacaagta tattaatgcg tgctaagccc
     2101 catgaagtaa aactgatgat gatcgatccg aaaatggttg aattgaatat gtacaacggc
     2161 atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa aagcagcgca agccttacaa
     2221 aaagtagtac aagaaatgga agaacgatat gaaaaattcg cggcaaccgg agtaagaaat
     2281 atttctggtt acaatgaatt tgttcaacag aaaaacttgg aaaacggaac gaaacatcca
     2341 accttaccat tcatcgtagt gatcgtagat gaattagctg atttgatgat ggtagccagc
     2401 aatgaagttg aagatgcgat tattcgttta gcgcaaatgg ctcgggcagc ggggattcat
     2461 atgatcctag ctactcaacg tccaagtgtg gatgttatca caggaatcat caaagcaaat
     2521 gtaccttcca gaatggcatt tgcggtatca agcggtacag attctcggac gatcattgat
     2581 tctaatgggg cagaaaagct cttagggcga ggagatatgc tattcttgcc aatgggtgag
     2641 aataaaccga tccgtgtgca aggagcattc atttctgacc acgaagtaga acgggtcgtt
     2701 caatttgtta cagatcagca agaagctcat tatgaagaga aaatgatgcc gacagatgaa
     2761 gttgaaacga caggtgctcc agaacagcca caagatgagc tgtttgaaga agcaaaagca
     2821 cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc aaagaagatt tcggatcggt
     2881 tacaaccgag cagcacgttt agtagatgaa ctggaagctc atggagttgt tggcccgtca
     2941 gaaggaagca agcctcggaa agtattcatt gagcaggaag aaacatcaga aacgttgccg
     3001 gacgataatg acatgtaaaa cttgtgtgat gaagggttga aaaacgaata accaataaaa
     3061 agacaaggca tgatgatacc aaaagaaaaa atggtttttt tccatctttt ctaatacggt
     3121 atgatgtctt gtctttttag tttttgtgag attaaagttc tattcgattt atttgtgaag
     3181 tattgcatta aggaaatgaa tcatgtatag tatgtagcga aactttgaga aaaagaggga
     3241 aaatactttg gaaaaacagt ccatttatgg cttgacgaat gaagaattaa tcaattggtt
     3301 tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt tgggaatggt tgtatcaaaa
     3361 acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa caattgatcg aaaaattatc
     3421 ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc caagaagctt cggatggtac
     3481 agtaaaatat ttgtttgaac tccctgataa aaacatgatt gagactgtac tgatgagaca
     3541 agaatatggc ttgtcagttt gtgtgacgac acaagtagga tgtaatatcg gctgtacgtt
     3601 ttgtgcaagc gggctgctca aaaagaaccg tgatctgact gctggagaaa ttgttgcaca
     3661 gatcatgatg gtccaacatt attttgatga gcgcaatctt ggcgaacgcg tttcacatgt
     3721 cgttgtaatg gggatcggtg agccatttga taattacgac aatgtaatgg actttttgca
     3781 tatcatcaat gatgccaaag gattggcaat tggtgctcgg cacattacag tttccactag
     3841 tggattagca cacaaaatca aggaattcgc taataacggc ttgcaagtca atctggctat
     3901 ttccttgcac gcgccgaata atgaggtgcg aacatcgatc atgcggatca accgaagttt
     3961 cccaattgag aaattgatgg aagctgtgga tgagtattta gagaaaacaa accgtcgaat
     4021 cacgtttgaa tatatcatgt tgaatcaagt aaatgataga ccagagcatg cgcaacaatt
     4081 agcagatcta ttaaaagata agaaaaaatt agcatatgtc aacttgattc catataatcc
     4141 agtcagtgaa catgatcaat attctcgcag tccaaaagct gatgtattga gattttatga
     4201 tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa gaacatggta cagatattga
     4261 tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa acagttgcca aataatcgta
     4321 tcctactcgt tttttcaatt accataaaag gagcagtttt cttaattaag gaactgttca
     4381 acttaaataa gagaaaagat tctatagcga aagctataaa gctttttttg acaagaaaaa
     4441 ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc ctgcttgatg ggggttttag
     4501 caattattta actgattttc ttatatcctt gctggctttc ttgtgtagca tgataagttt
     4561 ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat ttcataaggc acatgtttaa
     4621 tatcatttgt tgccatggct ttatctaaga ttgcttctaa atcggtcgtg acattgagat
     4681 tcatgtcggt taacgatact ggaagttggt tctttttcat aaacacatag attttatcaa
     4741 acaaatcgaa ctgctgatcc atcatcaata aggtcagtac accataactg acagatgctc
     4801 cgtgaaggta tccctccatt ttttctagaa cagtcgtacc gtaatagaat gagtgtgcca
     4861 tagcagaatt atagttattg ttaactaaaa cagaaaccaa tccggtagta acaatgatat
     4921 ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc ggcactatct ttcaatgctt
     4981 gcgtaccata agtcaagaga cgatcaatgc acatttttcc gatttggatt cctaatctat
     5041 ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga ttcgacttct ttggataaag
     5101 catcgccaat acctgcccat aagtatttat caggtgcttc ggcaataatt gctgtatcaa
     5161 tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc gtccattacg ccttcttcgt
     5221 tatagatgac acataccgca gtgacgggcg aacagttcga accaatagtg ggaaaggtga
     5281 agactggttt agctagtttt tcaccaatga cttttacagt atcgcaagca cggccaccac
     5341 cgactgcaaa caggacgtct gctttttgga cactttcatg ctggatcaac tgttcaatat
     5401 ttccataagt tgaattaccc ccgtaccaga gactggcgac aacctctatt tcctgtgtca
     5461 aagctacttt tattttgttt tctgcctttg aaagtgcagt cttccctcca atcagcacta
     5521 cttttgtccc atattctttg acgattccat ttgctttttc atacgcatct gttccaatgg
     5581 tataatttgg taagaaaaca ctttgactca tattcatttc cttctttctt cgttgaaacg
     5641 ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt agatggagta gaggtgaacc
     5701 taagacagga taagtgatga ataaggtggc atgaacacct tgaaatcatc aataagtatc
     5761 ctaaaaacga acaagtacaa aaacattgaa aacgacaata atgaattttt gttcctctta
     5821 gtatttctac cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat
     5881 tattatatga aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga
     5941 taatctatat catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct
     6001 ttcctatttt gaataaagtg tttctgtata atttttttat tttataagta agataaataa
     6061 tgaaagaata gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt
     6121 tcaaaaggac tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt
     6181 tgaccgtaat aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc
     6241 ggaagaatat ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat
     6301 cgcggtagca gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata
     6361 cggtatcaca ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca
     6421 aataggtact cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac
     6481 gatttattta ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta
     6541 tgagcataca tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt
     6601 ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa
     6661 accggatatt tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga
     6721 ggttgtgaca gaagtaatca gcttcaagaa ataagaagga atttacgaaa attactt
//
LOCUS       DACCQI010000279         6391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.141, whole genome shotgun sequence.
ACCESSION   DACCQI010000279 DACCQI010000000
VERSION     DACCQI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.141"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            74..190
                     /locus_tag="IVO76_002960"
     CDS             74..190
                     /locus_tag="IVO76_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7430863.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            206..2125
                     /gene="tet(M)"
                     /locus_tag="IVO76_002961"
     CDS             206..2125
                     /gene="tet(M)"
                     /locus_tag="IVO76_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP7430864.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVTCALLQEKYHVEVKIKKPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYDDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRNDLTFFTNGRSVCLTELKGYQVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2240..2290
                     /locus_tag="IVO76_002962"
                     /pseudo
     CDS             2240..2290
                     /locus_tag="IVO76_002962"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2324..3700
                     /gene="tet(L)"
                     /locus_tag="IVO76_002963"
     CDS             2324..3700
                     /gene="tet(L)"
                     /locus_tag="IVO76_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAP7430865.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTALYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FPILILARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTAAFFLEVTPWLLTIILVFVFGGLSFTKTVISTIVSSSLKQKEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSVRLLNQKLLPMDLDQSTYLYSNMLFLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            4264..5526
                     /locus_tag="IVO76_002964"
     CDS             4264..5526
                     /locus_tag="IVO76_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAP7430866.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5771..6310
                     /locus_tag="IVO76_002965"
     CDS             5771..6310
                     /locus_tag="IVO76_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAP7430867.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHS"
BASE COUNT         2172 a          937 c         1307 g         1975 t
ORIGIN      
        1 ggaacaaata attagatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
       61 atacctttat catgtgattc taaagtatcc agagaatatc tgtatgcttt gtatacctat
      121 ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt
      181 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
      241 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
      301 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
      361 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
      421 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
      481 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
      541 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
      601 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctatggaaat
      661 tataatcaaa cagaaagtag agctgcaccc taatatgtgt gtgatgagct gtacggaacc
      721 tgagcaatgg gatgtggtaa tagaaggaaa tgatgacctt ttagagaaat atatgtccgg
      781 taaatcatta gaagcattag aactcgaaca agaggaaatc agaagatttc agaattgctc
      841 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
      901 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
      961 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     1021 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     1081 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     1141 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     1201 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     1261 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     1321 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     1381 gaaagtacaa atggaagtga cttgtgctct attgcaagaa aagtatcatg tggaggtaaa
     1441 aataaaaaag cctacagtca tttatatgga aagaccgtta aaaaaagcag agtataccat
     1501 tcacatcgaa gtgccaccga atcccttctg ggcttccatt ggtttatctg tatcaccgct
     1561 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     1621 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     1681 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     1741 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagttttaa aaaaagctgg
     1801 aacagaattg ttagagccat atcttagttt taaaatttat gcaccacaag aatatctttc
     1861 acgagcatat gacgatgctc ctaaatattg tgcgaacatc gtagacactc aactgaaaaa
     1921 taatgaggtc attcttagtg gagaaattcc tgctcggtgt attcaagaat atcgtaatga
     1981 tttaactttc tttacaaatg gacgtagcgt ttgtttaaca gagttaaaag gatatcaggt
     2041 taccactggc gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     2101 atatatgttc aataaaataa cttagcacat tttatattgt tatataaatg tggcttcttg
     2161 ttaaacgaaa cgagatgaaa tattttttaa taaagatttg aattaaagtg taaaggagga
     2221 attaattatg aagtaaaatg aatgtaacag ggttcaatta aaagagggaa gcgtatcatt
     2281 aaccctataa actacgtctg ccctcattat tggagggtga aatgtgaata catcctattc
     2341 acaatcgaat ttacgacaca accaaatttt aatttggctt tgcattttat ctttttttag
     2401 cgtattaaat gaaatggttt tgaacgtctc attacctgat attgcaaatg attttaataa
     2461 accaccggcg agtacaaact gggtgaacac agcctttatg ttaacctttt ctattggaac
     2521 agctctatat ggaaagctat ctgatcagct aggcattaaa aggttactcc tatttgggat
     2581 tataataaat tgcttcggat cggtaattgg gtttgttgga cattctttct ttcccatact
     2641 tattctggct cgatttattc aaggagctgg tgcagctgca tttccagcac tcgtgatggt
     2701 tgtagttgcg cgctatattc caaaggaaaa taggggtaaa gcatttggtc ttattggatc
     2761 gatagtagcc atgggagaag gagtcggtcc agcgattggt ggaatgatag cccattatat
     2821 tcattggtcc tatcttctac tcattcctat gataacaatt atcactgttc cgtttcttat
     2881 gaaattatta aagaaagaag taaggataaa aggtcatttt gatatcaaag gaattatact
     2941 aatgtctgta ggcattgtat tttttatgtt gtttacaaca tcatatagca tttcttttct
     3001 tatcgttagc gtgctgtcat tcctgatatt tgtaaaacat atcaggaaag taacagatcc
     3061 ttttgttgat cccggattag ggaaaaatat accttttatg attggagttc tttgtggggg
     3121 aattatattt ggaacagtag cagggtttgt ctctatggtt ccttatatga tgaaagatgt
     3181 tcaccagcta agtactgccg aaatcggaag tgtaattatt ttccctggaa caatgagtgt
     3241 cattattttc ggctacattg gtgggatact tgttgataga agaggtcctt tatacgtgtt
     3301 aaacatcgga gttacatttc tttctgttag ctttttaact gctgcctttt ttttagaagt
     3361 aacaccatgg ttactgacaa ttatattagt ttttgttttt ggtgggctgt cattcaccaa
     3421 aacagttata tctacaattg tttcaagtag tttgaaacaa aaggaagctg gtgccggaat
     3481 gagtttgctt aattttacca gctttttatc agagggaaca ggtattgcaa ttgttggtgg
     3541 tttattatct gtacgcttgc tgaatcagaa gttgctacct atggaccttg atcaatccac
     3601 ttacttgtat agtaatatgc tattcctttt ttcaggaatc attgtcatta gttggctggt
     3661 taccttgaat gtatataaac attctcaaag ggatttctaa atcgttaagg gatcaacttt
     3721 gggagagagt tcaaaattga tccttttttt ataacaggaa ttcaaatctt tttgttccat
     3781 taaagggcgc gattgctgaa taaaagatac gagagacctc tcttgtatct tttttatttt
     3841 gagtggtttt gtccgttaca ctagaaaacc gaaagacaat aaaaatttta ttcttgctga
     3901 gtctggcttt cggtaagcta gacaaaacgg acaaaataaa aattggcaag ggtttaaagg
     3961 tggagatttt ttgagtgatc ttctcaaaaa atactacctg tcccttgctg atttttaaac
     4021 gagcacgaga gcaaaacccc cctttgctga ggtggcagag ggcaggtttt tttgtttctt
     4081 ttttctcgta aaaaaaagaa aggtcttaaa ggttttatgg ttttggtcgg cactgccgac
     4141 agcctcgcag agcacacact ttatgaatat aaagtatagt gtgttatact ttacttggaa
     4201 gtggttgccg gaaagagcga aaatgcctca catttgtgcc acctaaaaag gagcgattta
     4261 catatgagtt atgcagtttg tagaatgcaa aaagtgaaat cagctggact aaaaggcatg
     4321 caatttcata atcaaagaga gcgaaaaagt agaacgaatg atgatattga ccatgagcga
     4381 acacgtgaaa attatgattt gaaaaatgat aaaaatattg attacaacga acgtgtcaaa
     4441 gaaattattg aatcacaaaa aacaggtaca agaaaaacga ggaaagatgc tgttcttgta
     4501 aatgagttgc tagtaacatc tgaccgagat ttttttgagc aactggatcc aggagaacaa
     4561 aaacgatttt ttgaggaaag ttataaatta ttttccgaac gatatggcaa gcaaaatatt
     4621 gcttatgcaa cagttcataa tgatgagcaa acccctcaca tgcatttagg tgttgtgcct
     4681 atgcgtgatg gaaaactgca aggaaaaaat gtgtttaatc gtcaagaact gttatggcta
     4741 caagataaat tccccgagca tatgaaaaaa cagggttttg agttgaagcg tggtgaacgt
     4801 ggctctgacc gtaaacatat tgagacagct aaatttaaaa aacaaacttt ggaaaaagag
     4861 attgattttc tagaaaaaaa tttagcagtt aaaaaagatg aatggactgc ttatagcgat
     4921 aaagttaaat cagatttaga agtaccagcg aaacgacaca tgaaaagtgt tgaagtgcca
     4981 acgggtgaaa agtccatgtt tggtttggga aaagaaataa tgaaaacaga aaagaaacca
     5041 accaaaaatg ttgttatatc ggagcgtgat tataaaaact tagtgactgc tgcgagagat
     5101 aacgataggt taaaacagca tgttagaaat ctcatgagta ctgatatggc gagagaatat
     5161 aaaaaattaa gtaaagaaca tgggcaagtt aaagaaaaat atagtggtct tgtagagcga
     5221 tttaatgaaa atgtaaatga ttataatgag ttgcttgaag aaaacaagtc tttaaagtct
     5281 aaaataagcg atttaaagcg tgatgtgagt ttaatctatg aaagcactaa ggaattcctt
     5341 aaggaacgta cagacggctt aaaagccttt aaaaacgttt ttaaggggtt tgtagacaag
     5401 gtaaaggata aaacagcaca attccaagaa aaacacgatt tagaacctaa aaagaacgaa
     5461 tttgaactaa ctcataaccg agaggtaaaa aaagaacgaa gtcgagatca gggaatgagt
     5521 ttataaaata aaaaaagcac ctgaaaaggt gtcttttttt gatggttttg aacttgttct
     5581 ttcttatctt gatacatata gaaataacgt catttttatt ttagttgctg aaaggtgcgt
     5641 tgaagtgttg gtatgtatgt gttttaaagt attgaaaacc cttaaaattg gttgcacaga
     5701 aaaaccccat ctgttaaagt tataagtgac caaacaaata actaaataga tgggggtttc
     5761 ttttaatatt atgtgtccta atagtagcat ttattcagat gaaaaatcaa gggttttagt
     5821 ggacaagaca aaaagtggaa aagtgagacc atggagagaa aagaaaatcg ctaatgttga
     5881 ttactttgaa cttctgcata ttcttgaatt taaaaaggct gaaagagtaa aagattgtgc
     5941 tgaaatatta gagtataaac aaaatcgtga aacaggcgaa agaaagttgt atcgagtgtg
     6001 gttttgtaaa tccaggcttt gtccaatgtg caactggagg agagcaatga aacatggcat
     6061 tcagtcacaa aaggttgttg ctgaagttat taaacaaaag ccaacagttc gttggttgtt
     6121 tctcacatta acagttaaaa atgtttatga tggcgaagaa ttaaataaga gtttgtcaga
     6181 tatggctcaa ggatttcgcc gaatgatgca atataaaaaa attaataaaa atcttgttgg
     6241 ttttatgcgt gcaacggaag tgacaataaa taataaagat aattcttata atcagcacat
     6301 gcattcttaa tcacttggat atattctttt tgttttaacg cttcaataat acttgctctt
     6361 gtcgcttctg ttccgatccc ctcgacttct t
//
LOCUS       DACCQI010000280         4311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.165, whole genome shotgun sequence.
ACCESSION   DACCQI010000280 DACCQI010000000
VERSION     DACCQI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.165"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..338
                     /locus_tag="IVO76_002966"
     CDS             <1..338
                     /locus_tag="IVO76_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7430868.1"
                     /translation="WPFYFYTIIRTLSKYYIAGHVFRIISCLNQVLFACNNAYCINEK
                     KAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNE
                     ESSDERKQI"
     gene            319..1053
                     /locus_tag="IVO76_002967"
     CDS             319..1053
                     /locus_tag="IVO76_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7430869.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1086..1949
                     /locus_tag="IVO76_002968"
     CDS             1086..1949
                     /locus_tag="IVO76_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003109014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7430870.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHLNQILRFELLRMMSWKVGIKTEFSLSVGKNYKYINKYID
                     EDLWNRLLSTYRMDSYENIWKSLFICHQLFREVSKEVAELLGFDYPEYGKNITRYTED
                     MYKKYVENDYF"
     gene            1993..2520
                     /locus_tag="IVO76_002969"
     CDS             1993..2520
                     /locus_tag="IVO76_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7430871.1"
                     /translation="MESTDVINEIRGILPRNSIGKYDLLTIFTHKTVFDKIVKVLSLD
                     FQNKVDYVAAPEAIGWILGTAIAKELGVGFIGVRKGDKLPYAKEEIISTHFTDYSGQD
                     KSFEISKSSIVRNKRVLIVDDWIETGSQMKALIALLEKLDCSIIGLATIGIDINEVTQ
                     KWIDSSFVAYIGSNI"
     gene            2748..4067
                     /locus_tag="IVO76_002970"
     CDS             2748..4067
                     /locus_tag="IVO76_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSsu5 family transposase"
                     /protein_id="HAP7430872.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGRLSSDSGLVLVKELMNTFGFSH
                     LAKQWLHITDHRAYFTHDNLAILEQLIMQLIAGYSADSSANLLRRDPVFQAVLGKKQL
                     ASQSSISRFLDRFTEENIDQLQALNQSLIDKARLIRNDTELIIDVDSTHSDTFGRQEQ
                     TDYNAHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKAFINPLLHHYKSTLPH
                     TEILVRGDSGFATPEVYESCEATESQYVIRLKSNRRLSQLAEHSVLYGDNQEWEEREV
                     QYFSLPYQAQSWSKPRRVCIRSVREGGELLFHHAFIVTNLSDNVSPEVIFSLYGKRGT
                     MENFIKEAKGGFYFDKTDSPRFLENHVRMMISVLAYNLVNFLKSIGFDKVNQGMTIHS
                     IRLILLKVAGKLVQTGRQVYLKLSSYHVYQTEFYKVFERLRRSKQWI"
BASE COUNT         1459 a          681 c          871 g         1300 t
ORIGIN      
        1 tatggccttt ttacttttac acaattatac ggactttatc taaatattat attgcaggcc
       61 atgtttttcg tattatttca tgcttaaatc aagtactatt tgcatgtaat aatgcttatt
      121 gcattaacga aaagaaagct ataaaactgc ttgaaacttt tgaatataaa cctgaaaaat
      181 atgccgaaag agttaatcat atttttgaag tactcggtct ttctcttttt gaatgctacg
      241 atatgaccga gaaactttat aaagaagtga atgaaattgt atcggagata aataactttt
      301 taaacgagga gagttcagat gaaagaaaac aaatatgatg ataatatatt ttttcaaaaa
      361 tacagtcaaa tgagtcgctc gcagaaagga ctggctggtg cgggagaatg ggagactttg
      421 aaaaagatgc tacctgattt taagggtaag cgtgtgcttg atttaggatg cggctatgga
      481 tggcactgta tatatgcgat ggaaaacggt gcttcctctg tagtaggtgt tgatatttct
      541 cataaaatgc tcgaagtagc aaaaggaaaa acccattttc cacagattga atatgaatgc
      601 tgtgccatag aagatgtgga tttcccagag gagagctttg atgtaatact aagttcgctt
      661 gcgtttcatt atgtagcaga ctatgagaat ttaataaaaa agatatatag gatgctgaag
      721 gctggtggca atttagtttt tacagttgaa catcctgttt ttactgctca tggaacacaa
      781 gactggtatt ataacgaaaa aggagaaata ctgcatttcc cggtggacaa ttattattat
      841 gagggcaaac ggacagctat gtttttggaa gaaaaggtta caaaatatca tagaacactg
      901 accacatatc taaatacact gctttcaaat agttttataa taaatcagat tgtggagcca
      961 cagccgccag agaacatgat ggatattccg gggatggcgg atgaaatgcg acgcccaatg
     1021 atgctgattg tatcggcaaa aaagaagatg taataatata gaaaaaataa acgaggagta
     1081 tgtaaatgag atcagaaaaa gaaatgatgg atttagtact ttctttagca gaacaggatg
     1141 aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa tattaatata cctaaagatg
     1201 aatttcagga ttatgatatt acatattttg taagtgatat agaaccgttt atatctaatg
     1261 atgactggct taatcaattt gggaatataa taatgatgca aaagccggag gatatggaat
     1321 tattcccacc tgaagaaaag ggattttcct atcttatgct atttgatgat tacaataaaa
     1381 ttgatcttac cttattgccc ttggaagagt tagataatta cctaaagggc gataaattaa
     1441 taaaggttct aattgataaa gattgtagaa ttaaaaggga catagttccg actgatatag
     1501 attatcatgt aagaaagcca agcgcaaggg agtatgatga ttgctgcaat gaattttgga
     1561 atgtaacacc ttatgttatt aaaggattgt gccgcaaaga gatactgttt gcaatcgatc
     1621 atctgaacca gattctacgg tttgaactac ttaggatgat gtcgtggaag gttgggataa
     1681 agacagaatt ttcattaagt gttgggaaaa attataagta tattaacaaa tacattgatg
     1741 aagatctatg gaatagatta ttatctacat atcgcatgga ttcctatgaa aatatttgga
     1801 agtcattatt tatatgccac caattgttca gggaagtgtc caaagaggta gcagaactac
     1861 tggggtttga ttatccagag tatggtaaga acataacaag atataccgag gacatgtata
     1921 aaaaatatgt tgaaaatgac tatttttaaa gattagcaaa gattattaac cataagcaag
     1981 gggtgtaaat gtatggaaag tactgatgtt ataaatgaaa ttagaggtat attacctcgt
     2041 aactcaattg gaaaatatga tttactaact atttttacac ataaaaccgt ttttgataaa
     2101 attgtaaaag tactatcatt ggattttcaa aataaagtag attatgtggc tgccccagaa
     2161 gcaattggat ggatactagg cactgccata gccaaagaac ttggagtggg gtttattgga
     2221 gtaagaaaag gtgataagct gccatacgca aaagaagaga ttatttcgac acattttacc
     2281 gactattcag gtcaagataa aagctttgaa atatctaaga gttctattgt gaggaacaaa
     2341 agagttttga ttgtagatga ttggatagag acaggttctc aaatgaaggc tttaatagct
     2401 ttgttagaga aattggattg cagcattatt gggttggcta ctattgggat tgatataaat
     2461 gaggttactc aaaaatggat agacagcagt tttgtagctt atattggctc aaacatataa
     2521 taaaccgaaa gaaaatcttg aatcaaaatt ttggatggcg ttaatttaca atacaacgaa
     2581 agatgtttac cctgaataat tcatactttt taattgaaac cgtctgaaac tgcctaacgg
     2641 cagtctgata attacttttt tagtttcacc ccttaagtga tgaaaaaaga accccattct
     2701 gctatggtaa aggtgacgaa accaaccata agaaaggagt tcttctcatg actagcttac
     2761 acaaaaatca agttaaattc aattcaaata tcactatttc acataccggt ggtcgtctat
     2821 caagtgattc ggggttagtc ctggttaaag aattaatgaa taccttcggc ttttcgcact
     2881 tagctaaaca atggctccat attacagatc atcgggctta ttttacccat gataacttag
     2941 cgatattgga acagcttatt atgcaattaa ttgctggtta ctcggcagac tcatcagcta
     3001 atctattaag acgggatcct gtctttcaag ctgtactcgg taaaaaacag ttggcatcac
     3061 aatcctcaat ctcacggttt ctggatcgtt tcaccgagga gaacatcgat caactccaag
     3121 cattaaatca gtcattgatt gataaagcac gtttaattcg caatgacacc gagttaatca
     3181 ttgatgtcga ttccacacat tcggatacct ttggacgcca agaacaaaca gattataatg
     3241 cccattatca aacctacggc tatcacccat tagttgcctt tgatggactg accggagatt
     3301 tcttaaaagc tgaactgcgt tcaggcaatc agtacacatc taaaggggta aaagccttta
     3361 tcaacccgct tttacaccac tacaagagca cgttacctca taccgagata ttggttcggg
     3421 gtgacagcgg cttcgccaca ccagaggtgt atgagtcttg tgaagcaact gaaagccagt
     3481 acgttatccg attaaagagc aatcggaggt taagtcagtt agctgaacat tctgttcttt
     3541 acggggacaa tcaagaatgg gaagagcgag aagtgcagta tttttcacta ccttatcaag
     3601 cacaatcctg gtcgaaacct cgtcgtgtct gtatacgctc cgtccgtgaa ggaggagaat
     3661 tgctatttca tcatgcgttt attgtgacta atctatccga caatgtctcg cctgaagtca
     3721 tattttctct ttacggcaag cgaggaacaa tggaaaattt catcaaagaa gcgaaaggtg
     3781 gcttttactt cgataaaaca gatagcccgc gtttcttaga aaatcatgtc agaatgatga
     3841 tcagtgtcct tgcatacaac ctcgttaatt ttttaaaaag cattggtttt gataaagtga
     3901 accagggcat gacgattcat tccatccggc tgatattgct taaagtagcc ggtaaacttg
     3961 ttcagactgg gagacaggtc tatctcaaac tgtcaagtta tcatgtgtat caaactgaat
     4021 tttataaggt ttttgaacgc ctgcggcgat ccaaacaatg gatttagact agttatcaca
     4081 caaatttttt aaccgattct ttttcaaggg attcgtctgc ccttaaatag acaaataatt
     4141 tttattatga tcttcttccg taggcatcct gttcgaaaac acattatttt ggaagaaagt
     4201 atctgcctaa tttaaaaatt ggcaatttgt tcaaaaaaac aggttaaatt tagagctatg
     4261 aattattcag gttttttcga ggtggaagct agtataaaag taaaccaagg g
//
LOCUS       DACCQI010000281         4176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.166, whole genome shotgun sequence.
ACCESSION   DACCQI010000281 DACCQI010000000
VERSION     DACCQI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.166"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            127..1167
                     /locus_tag="IVO76_002971"
     CDS             127..1167
                     /locus_tag="IVO76_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7430873.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1265..1453
                     /locus_tag="IVO76_002972"
     CDS             1265..1453
                     /locus_tag="IVO76_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7430874.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1511..1864)
                     /locus_tag="IVO76_002973"
     CDS             complement(1511..1864)
                     /locus_tag="IVO76_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430875.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2395..2817
                     /locus_tag="IVO76_002974"
     CDS             2395..2817
                     /locus_tag="IVO76_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7430876.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2814..3044
                     /locus_tag="IVO76_002975"
     CDS             2814..3044
                     /locus_tag="IVO76_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7430877.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3542..3742
                     /locus_tag="IVO76_002976"
     CDS             3542..3742
                     /locus_tag="IVO76_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7430878.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3769..>4176
                     /locus_tag="IVO76_002977"
     CDS             3769..>4176
                     /locus_tag="IVO76_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7430879.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGL"
BASE COUNT         1415 a          671 c          776 g         1314 t
ORIGIN      
        1 tacattttgt cattttgtac tccaaaactg aactacttac ttgcatatat acgcctttat
       61 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
      121 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
      181 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
      241 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
      301 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
      361 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
      421 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      481 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      541 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      601 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
      661 tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt
      721 tggaatgtga cggattgtaa aatctgtttt aagtacggtt tatactatag ccctgttagt
      781 actccagcag attttcggat gcttactcct attgtactgg agcaagcctt tagaaaagct
      841 ggaacagaat tgttagagcc atatcttagt tttaaagttt atgcaccaca ggaatatctt
      901 tcacgggcat ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac tcaactgaaa
      961 aataatgagg tcattattat tggagaaatt cctgctcgat gtattcaaga ttatcgcaat
     1021 gatttaactt tttttacaaa tgggcttagt gtttgtttag cagagctaaa aggatatcag
     1081 gttaccactg gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat agataaagta
     1141 agatatatgt tcaataaaat aacttagtgc gttttatgtt gttatataaa tatggtttct
     1201 tattaaataa gatgaaatat tctttaatat agatttgaat taaagtggaa aggaggagat
     1261 tgttattata aactacaagt ggatattgtg tcctatttgt ggaaataaaa caagactacg
     1321 aatacgagtg gatactatac ttaaaaattt ccctttatac tgccccaaat gtaagaacga
     1381 aactttaatt aatgttcaaa aaatgaatat aataacaatc aaagagccag acgccaagac
     1441 gcagagccga taatttgaga aatgaaactc tcatcttatc ggctcttttt gtttatctga
     1501 attttactga ctagccttca atatttcttg cttcattgat accttttgct agtgcttcca
     1561 ttaaggatag ttctttgtct gtaaagctat ccatgtattt ttctacctgt aaccttcgag
     1621 tgcttttaat caaattatct gtaggtaaga aaaattcatc aatagataca tggagtaatg
     1681 atacaagatc atacagcact tgtgtacttg ggtgttgtcc tttgttttct atatttgtta
     1741 aataacgtgg gtcaatttca atcattgttc ccacttgttc acgagtgaga ccccgtttca
     1801 ttcgagcctc tttaatcgct aaaccaacgg ctctaaaatc gtatttatct tcttttttac
     1861 gcatactaaa tcacctctat acattttact gttcctatat aaccataaac aggtatagaa
     1921 aaacatgtta tatagtttat gagttcctat agaacactgt ggaatttatt aatgcgatga
     1981 atccaatttc aaatagaaag gtcgctgatt ttatttacct atacaacttt ataaaattta
     2041 gtcatactcc tagaaagtag ataaaattgg cgacttaatg gagggaaaag tcgccatgag
     2101 aaagataatc tgtcatctag ttctcttaat taagttctta tcaaaaaaac ttatccacct
     2161 gtactggata gatatgacca tcaatgtggt tttgcagatt acatagatag aaaaatcaaa
     2221 gcatatgaac agagaaaaca atctgtttat gtgctttttt ggtcacttaa taaatatctt
     2281 ttaggatttc tcttcattta tgcaacaaat cccccgttcg tgtcggggtt tagtatgaag
     2341 ggagataaag aagcacatgc cactcccttt cacgacagaa agggggtgaa aagcatgaaa
     2401 ccatcttctt ttcaagaaac catagaaaat cagtttgact atatctgcaa acaagttatt
     2461 gaagatgaac gaaaagatta cttcaaacat ctagacagac tatcagcaaa agaagtctcg
     2521 ttttctgata tagataacta ccttgtcaac tctttttcta ctgttgatac gtacgtcact
     2581 gatttccaac tatttaaatt atttggatta agaatcaatg ttgaaagtga tttattaagt
     2641 gaagccctgc gtcatctttc agagaaaaaa cggaacatta ttttacttca ttactttatg
     2701 gatatgagtg atgtggagat tgcggagtta ctaaatttga atcgttcgac tgtttatcgt
     2761 catcgaataa gtggactagc catgattaaa gaattcatga aggagtgtaa agaatgaaaa
     2821 cagcttatcc aagggttccc ttccccctca ttgtaaaggc gacagatggc gacgtggaag
     2881 caattaatca gattgttaaa cattatcgag ggtatacctc aaaacgttca ttacgtcgca
     2941 tgacagacga atatggaaat tctcatatgg ttattgatga aaccctacgt ggcagaatgg
     3001 aaacgaaact catctctaag attttatcgt tcgaaattaa atagtcccat tttttcgtgg
     3061 aagcgtgtcg aatcgtatcc acgcttcccg atttgggagg tttgttattc catcaaagca
     3121 tattgtgtgt tcagtaggtt ttgataggct aacaagccaa acgttctttg acaaccaaat
     3181 aaaggcaatc gaatacgttt cggtaagaaa agagctgatg aactaacacg ccatgaccta
     3241 caaaattagt gagcgattca tgttagtaat tcgaaagtaa tctttagcag gattgctgcc
     3301 atgactttct tatcgtgata atgatactcc cgtatagcca tagtccgagc gttagaagcg
     3361 tcacaggcaa tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc tgtgagcttt
     3421 gctagagctg ttcgattgct aagatattct tctcttgtca gtgtattatt ataaaactat
     3481 ttgatataat tataggtgaa tattggagtt ttcctagcct ataattggga aaggagtaat
     3541 aatgataacg gtcacaaaaa aggatttagt tgagctagga tatggaactt ctttttcagc
     3601 agacatcata aaaaaagcaa aagaattgat gattacaaaa gggcatatct actatcaatc
     3661 tcgaaagcta gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa ttcggttttc
     3721 aaatgaaaac aggtctgagt agtttttgca cagtaaggag taaaaactat ggttaaagac
     3781 ccaataaaaa aagcaaagaa tggaacctat tattttcgtg caaatctagg ttatgattct
     3841 aaagggaaaa aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga agcaagagaa
     3901 gaatattcga aactgctatt gatgaaacca gaagaattaa gtgagaataa ggataaaatg
     3961 acgtttaaac attatatctt tgaaattttc cttccatggt ataagacaca ggtaaagctc
     4021 agaacatatg aaaatcgctt accaactatt aagaagcatt tctcttattt tgataaaatg
     4081 gctgtttccg atattgagcc tattgatgtg caaaattggc aactaaaatt atcaaagaag
     4141 tgtaagtctt cttatgtaag agctgttcaa ggacta
//
LOCUS       DACCQI010000282         3277 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.191, whole genome shotgun sequence.
ACCESSION   DACCQI010000282 DACCQI010000000
VERSION     DACCQI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.191"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            276..773
                     /gene="dfrG"
                     /locus_tag="IVO76_002978"
     CDS             276..773
                     /gene="dfrG"
                     /locus_tag="IVO76_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7430880.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(845..2797)
                     /locus_tag="IVO76_002979"
     CDS             complement(845..2797)
                     /locus_tag="IVO76_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7430881.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2804..3100)
                     /locus_tag="IVO76_002980"
     CDS             complement(2804..3100)
                     /locus_tag="IVO76_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7430882.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1015 a          566 c          539 g         1157 t
ORIGIN      
        1 gtaagtagtt cagttttgga gtacaaaatg acaaaatgta cttcaaaatg gactactttt
       61 ttcattttaa cttacatacc aaattacata caaaatgatt tataaaatgc aattgaaaat
      121 gataattgaa tgaaaatttg tgattttgtg gtattatgat aatataactt atagaaaaaa
      181 ttgcttattc aactaacggg caggataatg agaaaaggtt ttttgataga aataagaaat
      241 tagatggtaa attagttaaa aaggctgtgt tataaatgaa agtttctttg attgctgcga
      301 tggataagaa tagagtgatt ggcaaagaga atgacattcc ttggaggatt cccaaggact
      361 gggaatatgt taaaaatact acaaagggac atccgataat attaggtagg aagaaccttg
      421 aatcaatcgg aagagcctta cctgacagaa gaaatattat tctgacgaga gataaggggt
      481 ttacctttaa tggttgtgaa attgttcatt caatagaaga tgtttttgag ttatgtaaaa
      541 acgaagaaga aatttttatt ttcggaggag aacagattta taatttgttt ttcccttatg
      601 ttgagaaaat gtacatcaca aaaatacatc atgaattcga aggagatact ttttttccag
      661 aagtgaatta tgaggaatgg aatgaggtat ttgcccaaaa agggataaag aatgataaaa
      721 atccgtataa ctactatttt catgtatatg aaagaaaaaa cttattgagt taacgggtgc
      781 gttaattgaa gaagaacagt aaagataaag cacaagagct tgggattact ccaagctctt
      841 tttgtcattt gaaataaatt atatctttcc aatcaaattg tttatctgta atccctaatc
      901 tttgagcagg tgtcaaaacc tttttatccg ctgatttaaa agggcgacaa aagttgtagt
      961 aagttcttag aattgtaatc gcaaattgag catacttcgg attgaagtta gcgtaaatat
     1021 agctcttctt atcaccacga gctgttgtta atggtctttc aagagatgat atataacgtc
     1081 ttatctgttg aataaagcta ttggtcgaat ggtcattaac atttaatagc atctttgcga
     1141 tttcgtttgg ttccagagca gataaatccg ttctacaatc aactgaataa aatcctttat
     1201 ccttagtggc taatggatgt ttgatagggt tatcggcata tttgcgatat ctttctccat
     1261 ctttactaat agcctcttcg tggaattgat gacttgttag taattcagtt agataatgag
     1321 atgccaaagt ccgtagaaac tttgttttga atcctctgat atctccccaa tcgagcagtt
     1381 ctgcttttgc ttgtccgaat tcttttaagc attgttttct gcttttcgtc ttgttaactt
     1441 ggcaaataaa atgatgtgca tcgctcaacc ttaattcttt cgtgaatact ctataaaagg
     1501 cgtttctaat agagaagtca tcatcactaa tcattcgcca ttcgtcggca ttaaccattt
     1561 gtttaattaa ccaatagtga gcagttgtag tataaggagc atcaacattt aaaccatcga
     1621 caaatctact cctattagta attttcccta attcgtgaag ataagcattc ctattttcgc
     1681 tatcgttagc ggatggttct tgtggataat aagaccaatc taacctatca tttttacgac
     1741 taaaagtatt gagatggtct tctttgaatt ttcttgtatc ttcatttaat tcatccattg
     1801 aaatattcca atcataggca acatcagaac ggaacacata tcttgataag acctcagcag
     1861 atacaactat atacgtttgc atattaaggt cttcaaaccc cgtatatttc tttgaacctt
     1921 gtcctttctt acggacgtta tttaggtagt agtgcatctt gtccgtatta agccacatct
     1981 cgttaaattt ctttgtttga aggggttgtg tctcatatcg ttctaaaaac tccaaacaac
     2041 gcctatacag ccactccagc ttatgatagt aggttccaac tccaattcct aaaatatcac
     2101 aagtacgact gactgaaacc cttccaacaa ccatcttagc gaacattgga agaatggtgt
     2161 tcttttgttg gtgataagta gtggtttctt ctctttttgg tagaacatta gtgaacttct
     2221 tacacgcctt acattgatag cgttgagatt tgcccctact tttccccctt ttgtaaaatt
     2281 gcttcggctc attgaatggg gttgattctt cttccgagca gccttcttta tgaaattgat
     2341 aatctggctc aacatcttgg atgctattga tttctataag gcgtttaatt tcttcaacaa
     2401 cagaccaatt agataaaggg gttactgtac agttttgcga tacacctctg ccaataggat
     2461 tgtcattgca gtacatacct ttatgacctt tatctttgga actaccagtc attttatatc
     2521 gacttggttt cccttttaca tctttgaatt tgtgctgttc tttcccaaaa ttacaacaaa
     2581 acggattgat gcagtaatta tattgaatgg gatattcaga accgttccat ttaaaagacg
     2641 ttggcaagaa caacattttg ttataccttg ttgcaaatcg ttttggagtt aataaatcat
     2701 attgtttagc cctatctttt aattcttcgt ttgaaatcgg aattttaaca tctacaattt
     2761 catcttcctt cgtggctaat cttttaagct tggtcattgt ctatcaaccc actctatctt
     2821 tgatacccat tttttcaaca atccgcttat tacttcgctt gttggcaatc cattcaataa
     2881 acttcttatc gtctaaaatg ttcaataaga attcatccac tgcttcgctt tcagtacgct
     2941 ctgcatattc agcgtattgc ttaactatca cccttacttg ctctgatatt tcccaatcca
     3001 cagacttagc gttcttattt tttggctcaa gaaattgcat aggaaaatgc accccttttt
     3061 gcttagtaaa ttgtaaccat tttaacacac caatttgcat tcctacaata attatttcac
     3121 gttcgcattt ttatatacat tttaacttac attttgtggt gtaaattaac aacaaacgtt
     3181 gatttaacaa taaaaaacga gtatcaaatt cagttgcaat ttgatactca tttaggttta
     3241 cattttcact ttttgtactc caaaactgaa ctactta
//
LOCUS       DACCQI010000283         1996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.217, whole genome shotgun sequence.
ACCESSION   DACCQI010000283 DACCQI010000000
VERSION     DACCQI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.217"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            273..384
                     /locus_tag="IVO76_002981"
                     /pseudo
     CDS             273..384
                     /locus_tag="IVO76_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            405..488
                     /locus_tag="IVO76_002982"
     CDS             405..488
                     /locus_tag="IVO76_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP7430883.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYVDK"
     gene            613..1350
                     /gene="erm(B)"
                     /locus_tag="IVO76_002983"
     CDS             613..1350
                     /gene="erm(B)"
                     /locus_tag="IVO76_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7430884.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1822..>1996
                     /locus_tag="IVO76_002984"
     CDS             1822..>1996
                     /locus_tag="IVO76_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7430885.1"
                     /translation="MELAFRSRLRQMRGNKTRKQFAEEIGMKESNYLKIELGKSNPTV
                     KTLERIAKLTNSTL"
BASE COUNT          733 a          300 c          343 g          620 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa atttcgctgt tattttgtac
       61 atttaacttg acggtgacat ctctctattg tgagttatta gtggtacagt tttcaaccgt
      121 tttaattata aaaaagtggt gcatttttaa attggcacaa acaggtaacg gttattgcag
      181 gtgtatttct tatctatggg tttaacatgg attttatcat taaaatcatg agtattgtcc
      241 gagagtgatt ggtcttgcgt atggttaacc ctaaagttat ggaaataaga cttagaagca
      301 aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg aattgaagtt
      361 aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg gtattccaaa
      421 tgcgtaatgt agataaaaca tctactgttt tgaaacagac taaaaacagt gattacgtag
      481 ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt tttaaacaga
      541 taactaaaat tacaaacaaa tcgtttaact tctgtattta tttatagatg taatcacttc
      601 aggagtgatt acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa
      661 gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt
      721 ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct
      781 attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact gaacattcgt
      841 gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa
      901 attgttggga atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa
      961 agccatgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat
     1021 attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag
     1081 ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc
     1141 cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa
     1201 tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa
     1261 cacgccaaag taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat
     1321 agttatctat tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa
     1381 gttacacgtt actaaaggga atgtagataa attattaggt atactactga cagcttccaa
     1441 ggagctaaag aggtccctag cgcctacggg gaatttgtat cgataaggaa tagatttaaa
     1501 aatttcgctg ttattttgta caataaggat aaatttgaat ggtaccataa acgaccgttt
     1561 atggtaccat ttcattttcc tgctttttct aaatggggtc ccgagcgctt agaatcgctt
     1621 taggaaacac gatccagtcc aataatcgtc gataaaaact tttgaaaaag gttggtgaaa
     1681 ttacctactt ttggaataat cacaaatcac aagtgattaa tcacaaatca caagtgatta
     1741 atcacttgtt tattaagata ttaaaagcta taatttaaaa taaagcgtga attttattac
     1801 acaaaaagag gggggagaaa cttggagcta gcattcagaa gtcgtttaag acaaatgaga
     1861 ggaaataaaa cacgtaaaca gtttgctgaa gaaattggaa tgaaagaatc taattactta
     1921 aaaattgagt taggaaaatc aaacccgact gtaaaaacac tggaacggat tgcaaaactt
     1981 acaaattcaa ccctgg
//
LOCUS       DACCQI010000284         1286 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.242, whole genome shotgun sequence.
ACCESSION   DACCQI010000284 DACCQI010000000
VERSION     DACCQI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.242"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            complement(<1..272)
                     /locus_tag="IVO76_002985"
     CDS             complement(<1..272)
                     /locus_tag="IVO76_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7430886.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKVAKIAEFLKRSRTPIYNVIHF
                     LKEGHTALDYYQQYKENKKRCGRQKIILPKEQQTYIKEKVNQGWTP"
     gene            481..1200
                     /gene="catA"
                     /locus_tag="IVO76_002986"
                     /pseudo
     CDS             481..1200
                     /gene="catA"
                     /locus_tag="IVO76_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type A chloramphenicol O-acetyltransferase"
BASE COUNT          408 a          200 c          216 g          462 t
ORIGIN      
        1 tcaggagtcc agccttgatt tactttttcc ttgatatagg tctgttgttc ttttggtaag
       61 atgattttct gtctgccaca ccgtttctta ttttctttat attgttggta gtaatcgagg
      121 gccgtatggc cttctttcaa gaaatgaatg acattataaa tgggtgttcg agatcgtttc
      181 aagaactcag caattttagc gactttaata ttttggtgat aataagactc tatcatcact
      241 aattcatccg tggtaagatg ggtgtaggtc attcgtgatc actcctatgt tttctttggt
      301 cggaaataca ttttgagtgt accacgaatg gcttttttat ttgtctagct taattttaca
      361 atcggcgtat atttatgtta caataatact gacttttaaa aaaggattga ttctaatgaa
      421 gaaagcagac aagtcagcct cctaaattca ctttagataa aaatttagga ggcatatcaa
      481 atgaacttta ataaaattga tttagacaat tggaagagaa aagagatatt taatcattat
      541 ttgaaccaac aaacgacttt tagtataacc acagaaattg atattagtgt tttataccga
      601 aacataaaac aagaaggata taaattttac cctgcattta ttttcttagt gacaagggtg
      661 ataaactcaa atacagcttt tagaactggt tacaatagcg acggagagtt aggttattgg
      721 gataagttag agccacttta tacaattttt gatagtgtat ctaaaacatt ctctggtatt
      781 tggactcctg taaagaatga cttcaaagag ttttatgatt tatacctttc tgatgtagag
      841 aaatataatg gttcggggaa attgtttccc aaaacaccta tacctgaaaa tgctttttct
      901 ctttctatta ttccatggac ttcatttact gggtttaact taaatgtcaa taataatagt
      961 aattaccttc tacccattat tacagcagga aaattcatta ataaaggtaa ttcaatatat
     1021 ttaccgctat ctttacaggt acatcattct gtttgtgatg gttatcatgc aggattgttt
     1081 atgaactcta ttcaggaatt gtcagatagg cctaatgact ggcttttata atatgagaca
     1141 atgccgactg tactttttac agtcagtttt ctaatgtcac taacctgccc gttaatttaa
     1201 gtgtactact tcacaaaacg cattgcttat tacatatgta ataagcaatg ccacaactct
     1261 taagttaaga ctataatgac aaatgt
//
LOCUS       DACCQI010000285         1041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.262, whole genome shotgun sequence.
ACCESSION   DACCQI010000285 DACCQI010000000
VERSION     DACCQI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.262"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            13..378
                     /locus_tag="IVO76_002987"
     CDS             13..378
                     /locus_tag="IVO76_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7430887.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            379..>1041
                     /gene="aac(6')-Ie"
                     /locus_tag="IVO76_002988"
     CDS             379..>1041
                     /gene="aac(6')-Ie"
                     /locus_tag="IVO76_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP7430888.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNN"
BASE COUNT          419 a           82 c          185 g          355 t
ORIGIN      
        1 attgatgata atatggtttt tgatattgat aatttaaaag gatttcttaa tgataccagt
       61 tcatttgggt ttatagctaa agaaaataat aaaattatag gatttgcata ttgctataca
      121 cttttaagac ctgatggaaa aacaatgttt tatttacact caataggaat gttacctaac
      181 tatcaagaca aaggttatgg ttcaaaatta ttatctttta ttaaggaata ttctaaagag
      241 attggttgtt ctgaaatgtt tttaataact gataaaggta atcctagagc ttgccatgta
      301 tatgaaaaat taggtggtaa aaatgattat aaagatgaaa tagtatatgt atatgattat
      361 gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa tatgtataag aactttaata
      421 gatgatgatt ttcctttgat gttaaaatgg ttaactgatg aaagagtatt agaattttat
      481 gatggtagag ataaaaaata tacattagaa tcattaaaaa aacattatac agagccttgg
      541 gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg atatggacaa
      601 atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac tgatgagata
      661 gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg aattggtaca
      721 agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa tgcagttatt
      781 ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc tggttttaga
      841 attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga ttgttattta
      901 atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata tttaattgag
      961 cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg ttatgatagt
     1021 gtggcatatt tagttaataa t
//
LOCUS       DACCQI010000286         1041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.263, whole genome shotgun sequence.
ACCESSION   DACCQI010000286 DACCQI010000000
VERSION     DACCQI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.263"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            13..378
                     /locus_tag="IVO76_002989"
     CDS             13..378
                     /locus_tag="IVO76_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7430889.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            379..>1041
                     /gene="aac(6')-Ie"
                     /locus_tag="IVO76_002990"
     CDS             379..>1041
                     /gene="aac(6')-Ie"
                     /locus_tag="IVO76_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HAP7430890.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNN"
BASE COUNT          418 a           82 c          186 g          355 t
ORIGIN      
        1 attgatgata atatggtttt tgatattgat aatttaaaag gatttcttaa tgataccagt
       61 tcatttgggt ttatagctaa agaaaataat aaaattatag gatttgcata ttgctataca
      121 cttttaagac ctgatggaaa aacaatgttt tatttacact caataggaat gttacctaac
      181 tatcaagaca aaggttatgg ttcaaaatta ttatctttta ttaaggaata ttctaaagag
      241 attggttgtt ctgaaatgtt tttaataact gataaaggta atcctagagc ttgccatgta
      301 tatgaaaaat taggtggtaa aaatgattat aaagatgaaa tagtatatgt atatgattat
      361 gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa tatgtataag aactttaata
      421 gatgatgatt ttcctttgat gttaaaatgg ttaactgatg aaagagtatt agaattttat
      481 ggtggtagag ataaaaaata tacattagaa tcattaaaaa aacattatac agagccttgg
      541 gaagatgaag tttttagagt aattattgaa tataacaatg ttcctattgg atatggacaa
      601 atatataaaa tgtatgatga gttatatact gattatcatt atccaaaaac tgatgagata
      661 gtctatggta tggatcaatt tataggagag ccaaattatt ggagtaaagg aattggtaca
      721 agatatatta aattgatttt tgaatttttg aaaaaagaaa gaaatgctaa tgcagttatt
      781 ttagaccctc ataaaaataa tccaagagca ataagggcat accaaaaatc tggttttaga
      841 attattgaag atttgccaga acatgaatta cacgagggca aaaaagaaga ttgttattta
      901 atggaatata gatatgatga taatgccaca aatgttaagg caatgaaata tttaattgag
      961 cattactttg ataatttcaa agtagatagt attgaaataa tcggtagtgg ttatgatagt
     1021 gtggcatatt tagttaataa t
//
LOCUS       DACCQI010000287          927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.270, whole genome shotgun sequence.
ACCESSION   DACCQI010000287 DACCQI010000000
VERSION     DACCQI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.270"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            117..851
                     /gene="erm"
                     /locus_tag="IVO76_002991"
     CDS             117..851
                     /gene="erm"
                     /locus_tag="IVO76_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HAP7430891.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYTKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          382 a          120 c          130 g          295 t
ORIGIN      
        1 gatgaggttg aacttttaca caagattgtg gacttgactc aaccaaacaa aaaataagtg
       61 gttataatgg attgttaata taaacattca ttataacctc ataggagtgg gttataatga
      121 acaaaaaaaa tataaaagat agtcaaaact ttattacttc aaagcatcat ataaatgaaa
      181 ttttgagaaa tgtacattta aatacaaatg ataatattat tgagattggt tcagggaaag
      241 gtcatttcac gtttgaatta gctaaaaggt gtaattatgt aaccgccatt gaaatagatc
      301 ctaaattatg taggataact aaaaacaaac ttattgaata tgagaacttc caggttatca
      361 ataaagatat tttacaattt aagtttccta aaaataagtc atataagata tatggaaata
      421 taccctacaa tataagtaca gatataattc gaaaaattgt ttttgaaagc acagctacag
      481 aaagttattt aatagtggaa tatggatttg ctaaaaggtt gctaaataca aatcgttcac
      541 tagcactatt tttaatgaca gaagttgata tatccatatt aagtaaaatc cctagagaat
      601 actttcatcc aaaacctaga gttaatagct cgttaattgt attaaaaaga cacccttcaa
      661 aaatatcact caaagataga aaacaatatg aaaattttgt tatgaaatgg gttaacaaag
      721 aatacacaaa actattttcc aaaaaccaat tttatcaagc cttaaaatat gcaagaattg
      781 acgatttaaa caatattagc tttgaacaat tcttgtctct tttcaatagc tataaattat
      841 ttaatagata agttaagggg tgcataaact gcatccctta acttatctgg ttctgttgca
      901 aagttttaaa tctactatca aataagg
//
LOCUS       DACCQI010000288          902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_080
            SAMN12916537-rid10366843.guided.271, whole genome shotgun sequence.
ACCESSION   DACCQI010000288 DACCQI010000000
VERSION     DACCQI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916537
            Sequence Read Archive: SRR10233602
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820157.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820157.1
            Genome Coverage       :: 28x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:37:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,992
            CDSs (total)                      :: 2,966
            Genes (coding)                    :: 2,893
            CDSs (with protein)               :: 2,893
            Genes (RNA)                       :: 26
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 18
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 25 of 73
            Pseudo Genes (internal stop)      :: 21 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916537-rid10366843.guided.271"
                     /isolate="RPH_EFM_080"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2011"
                     /collected_by="Murdoch University"
     gene            <1..817
                     /locus_tag="IVO76_002992"
                     /note="aph(2'')-Ia"
     CDS             <1..817
                     /locus_tag="IVO76_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP7430892.1"
                     /translation="GSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLE
                     TNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMH
                     GLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKK
                     CLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDIL
                     RMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          395 a           59 c          148 g          300 t
ORIGIN      
        1 cggtagtggt tatgatagtg tggcatattt agttaataat gaatacattt ttaaaacaaa
       61 atttagtact aataagaaaa aaggttatgc aaaagaaaaa gcaatatata attttttaaa
      121 tacaaattta gaaactaatg taaaaattcc taatattgaa tattcgtata ttagtgatga
      181 attatctata ctaggttata aagaaattaa aggaactttt ttaacaccag aaatttattc
      241 tactatgtca gaagaagaac aaaatttgtt aaaacgagat attgccagtt ttttaagaca
      301 aatgcacggt ttagattata cagatattag tgaatgtact attgataata aacaaaatgt
      361 attagaagag tatatattgt tgcgtgaaac tatttataat gatttaactg atatagaaaa
      421 agattatata gaaagtttta tggaaagact aaatgcaaca acagtttttg agggtaaaaa
      481 gtgtttatgc cataatgatt ttagttgtaa tcatctattg ttagatggca ataatagatt
      541 aactggaata attgattttg gagattctgg aattatagat gaatattgtg attttatata
      601 cttacttgaa gatagtgaag aagaaatagg aacaaatttt ggagaagata tattaagaat
      661 gtatggaaat atagatattg agaaagcaaa agaatatcaa gatatagttg aagaatatta
      721 tcctattgaa actattgttt atggaattaa aaatattaaa caggaattta tcgaaaatgg
      781 tagaaaagaa atttataaaa ggacttataa agattgatta tataatatat gaaaagctat
      841 tataaaagac attagtatta aatagtttaa aaaaatgaaa aataataaag gaagtgagtc
      901 aa
//
